Tacrine: A cholinesterase inhibitor that crosses the blood-brain barrier. Tacrine has been used to counter the effects of muscle relaxants, as a respiratory stimulant, and in the treatment of Alzheimer's disease and other central nervous system disorders.Central Nervous System Diseases: Diseases of any component of the brain (including the cerebral hemispheres, diencephalon, brain stem, and cerebellum) or the spinal cord.Demeclocycline: A TETRACYCLINE analog having a 7-chloro and a 6-methyl. Because it is excreted more slowly than TETRACYCLINE, it maintains effective blood levels for longer periods of time.Doxapram: A central respiratory stimulant with a brief duration of action. (From Martindale, The Extra Pharmocopoeia, 30th ed, p1225)Cholinesterase Inhibitors: Drugs that inhibit cholinesterases. The neurotransmitter ACETYLCHOLINE is rapidly hydrolyzed, and thereby inactivated, by cholinesterases. When cholinesterases are inhibited, the action of endogenously released acetylcholine at cholinergic synapses is potentiated. Cholinesterase inhibitors are widely used clinically for their potentiation of cholinergic inputs to the gastrointestinal tract and urinary bladder, the eye, and skeletal muscles; they are also used for their effects on the heart and the central nervous system.Respiratory System Agents: Drugs used for their effects on the respiratory system.CholinesterasesBrain: The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.Blood-Brain Barrier: Specialized non-fenestrated tightly-joined ENDOTHELIAL CELLS with TIGHT JUNCTIONS that form a transport barrier for certain substances between the cerebral capillaries and the BRAIN tissue.Nervous System Diseases: Diseases of the central and peripheral nervous system. This includes disorders of the brain, spinal cord, cranial nerves, peripheral nerves, nerve roots, autonomic nervous system, neuromuscular junction, and muscle.Acridine Orange: A cationic cytochemical stain specific for cell nuclei, especially DNA. It is used as a supravital stain and in fluorescence cytochemistry. It may cause mutations in microorganisms.Patents as Topic: Exclusive legal rights or privileges applied to inventions, plants, etc.Fluorescence Polarization: Measurement of the polarization of fluorescent light from solutions or microscopic specimens. It is used to provide information concerning molecular size, shape, and conformation, molecular anisotropy, electronic energy transfer, molecular interaction, including dye and coenzyme binding, and the antigen-antibody reaction.AcridinesFluorescence Polarization Immunoassay: Fluoroimmunoassay where detection of the hapten-antibody reaction is based on measurement of the increased polarization of fluorescence-labeled hapten when it is combined with antibody. The assay is very useful for the measurement of small haptenic antigens such as drugs at low concentrations.Intercalating Agents: Agents that are capable of inserting themselves between the successive bases in DNA, thus kinking, uncoiling or otherwise deforming it and therefore preventing its proper functioning. They are used in the study of DNA.RNA: A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)Foramen Ovale, Patent: A condition in which the FORAMEN OVALE in the ATRIAL SEPTUM fails to close shortly after birth. This results in abnormal communications between the two upper chambers of the heart. An isolated patent ovale foramen without other structural heart defects is usually of no hemodynamic significance.RNA, Viral: Ribonucleic acid that makes up the genetic material of viruses.Spectrometry, Fluorescence: Measurement of the intensity and quality of fluorescence.Nucleic Acids: High molecular weight polymers containing a mixture of purine and pyrimidine nucleotides chained together by ribose or deoxyribose linkages.Bromus: A plant genus of the family POACEAE. The name is similar to Broom or Scotch Broom (CYTISUS) or Butcher's Broom (RUSCUS) or Desert Broom (BACCHARIS) or Spanish Broom (SPARTIUM).DEAE-Cellulose: Cellulose derivative used in chromatography, as ion-exchange material, and for various industrial applications.Polydeoxyribonucleotides: A group of 13 or more deoxyribonucleotides in which the phosphate residues of each deoxyribonucleotide act as bridges in forming diester linkages between the deoxyribose moieties.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.DNA: A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Poly dA-dT: Polydeoxyribonucleotides made up of deoxyadenine nucleotides and thymine nucleotides. Present in DNA preparations isolated from crab species. Synthetic preparations have been used extensively in the study of DNA.Proteins: Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.Models, Molecular: Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.DNA Topoisomerases, Type II: DNA TOPOISOMERASES that catalyze ATP-dependent breakage of both strands of DNA, passage of the unbroken strands through the breaks, and rejoining of the broken strands. These enzymes bring about relaxation of the supercoiled DNA and resolution of a knotted circular DNA duplex.Antibiotics, Antineoplastic: Chemical substances, produced by microorganisms, inhibiting or preventing the proliferation of neoplasms.Anthracyclines: Organic compounds that have a tetrahydronaphthacenedione ring structure attached by a glycosidic linkage to the amino sugar daunosamine.Doxorubicin: Antineoplastic antibiotic obtained from Streptomyces peucetius. It is a hydroxy derivative of DAUNORUBICIN.Daunorubicin: A very toxic anthracycline aminoglycoside antineoplastic isolated from Streptomyces peucetius and others, used in treatment of LEUKEMIA and other NEOPLASMS.Topoisomerase II Inhibitors: Compounds that inhibit the activity of DNA TOPOISOMERASE II. Included in this category are a variety of ANTINEOPLASTIC AGENTS which target the eukaryotic form of topoisomerase II and ANTIBACTERIAL AGENTS which target the prokaryotic form of topoisomerase II.DNA Topoisomerases, Type I: DNA TOPOISOMERASES that catalyze ATP-independent breakage of one of the two strands of DNA, passage of the unbroken strand through the break, and rejoining of the broken strand. DNA Topoisomerases, Type I enzymes reduce the topological stress in the DNA structure by relaxing the superhelical turns and knotted rings in the DNA helix.Cross-Linking Reagents: Reagents with two reactive groups, usually at opposite ends of the molecule, that are capable of reacting with and thereby forming bridges between side chains of amino acids in proteins; the locations of naturally reactive areas within proteins can thereby be identified; may also be used for other macromolecules, like glycoproteins, nucleic acids, or other.Chromates: Salts of chromic acid containing the CrO(2-)4 radical.Quinacrine: An acridine derivative formerly widely used as an antimalarial but superseded by chloroquine in recent years. It has also been used as an anthelmintic and in the treatment of giardiasis and malignant effusions. It is used in cell biological experiments as an inhibitor of phospholipase A2.Quinacrine Mustard: Nitrogen mustard analog of quinacrine used primarily as a stain in the studies of chromosomes and chromatin. Fluoresces by reaction with nucleic acids in chromosomes.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Adsorption: The adhesion of gases, liquids, or dissolved solids onto a surface. It includes adsorptive phenomena of bacteria and viruses onto surfaces as well. ABSORPTION into the substance may follow but not necessarily.Kinetics: The rate dynamics in chemical or physical systems.Spectrophotometry: The art or process of comparing photometrically the relative intensities of the light in different parts of the spectrum.Spectrophotometry, Ultraviolet: Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Hydrogen-Ion Concentration: The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)LatviaIntegrative Medicine: The discipline concerned with using the combination of conventional ALLOPATHIC MEDICINE and ALTERNATIVE MEDICINE to address the biological, psychological, social, and spiritual aspects of health and illness.New HampshireNational Academy of Sciences (U.S.): A United States organization of distinguished scientists and engineers established for the purpose of investigating and reporting upon any subject of art or science as requested by any department of government. The National Research Council organized by NAS serves as the principal operating agency to stimulate and support research.USSRCongresses as Topic: Conferences, conventions or formal meetings usually attended by delegates representing a special field of interest.Ergot Alkaloids: Alkaloids originally isolated from the ergot fungus Claviceps purpurea (Hypocreaceae). They include compounds that are structurally related to ergoline (ERGOLINES) and ergotamine (ERGOTAMINES). Many of the ergot alkaloids act as alpha-adrenergic antagonists.MassachusettsIndole Alkaloids: Group of alkaloids containing a benzylpyrrole group (derived from TRYPTOPHAN)Claviceps: A genus of ascomycetous fungi, family Clavicipitaceae, order Hypocreales, parasitic on various grasses (POACEAE). The sclerotia contain several toxic alkaloids. Claviceps purpurea on rye causes ergotism.Nucleic Acid Probes: Nucleic acid which complements a specific mRNA or DNA molecule, or fragment thereof; used for hybridization studies in order to identify microorganisms and for genetic studies.Peptide Nucleic Acids: DNA analogs containing neutral amide backbone linkages composed of aminoethyl glycine units instead of the usual phosphodiester linkage of deoxyribose groups. Peptide nucleic acids have high biological stability and higher affinity for complementary DNA or RNA sequences than analogous DNA oligomers.Nucleic Acid Hybridization: Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.DNA Probes: Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.In Situ Hybridization, Fluorescence: A type of IN SITU HYBRIDIZATION in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. This staining is sufficiently distinct that the hybridization signal can be seen both in metaphase spreads and in interphase nuclei.Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.Microfluidic Analytical Techniques: Methods utilizing the principles of MICROFLUIDICS for sample handling, reagent mixing, and separation and detection of specific components in fluids.Microfluidics: The study of fluid channels and chambers of tiny dimensions of tens to hundreds of micrometers and volumes of nanoliters or picoliters. This is of interest in biological MICROCIRCULATION and used in MICROCHEMISTRY and INVESTIGATIVE TECHNIQUES.Dimethylpolysiloxanes: Silicone polymers which consist of silicon atoms substituted with methyl groups and linked by oxygen atoms. They comprise a series of biocompatible materials used as liquids, gels or solids; as film for artificial membranes, gels for implants, and liquids for drug vehicles; and as antifoaming agents.Lab-On-A-Chip Devices: Microdevices that combine microfluidics technology with electrical and/or mechanical functions for analyzing very small fluid volumes. They consist of microchannels etched into substrates made of silicon, glass, or polymer using processes similar to photolithography. The test fluids in the channels can then interact with different elements such as electrodes, photodetectors, chemical sensors, pumps, and valves.Microchip Analytical Procedures: The preparation and analysis of samples on miniaturized devices.PrintingEquipment Design: Methods of creating machines and devices.Miniaturization: The design or construction of objects greatly reduced in scale.Microchemistry: The development and use of techniques and equipment to study or perform chemical reactions, with small quantities of materials, frequently less than a milligram or a milliliter.Thioguanine: An antineoplastic compound which also has antimetabolite action. The drug is used in the therapy of acute leukemia.Purines: A series of heterocyclic compounds that are variously substituted in nature and are known also as purine bases. They include ADENINE and GUANINE, constituents of nucleic acids, as well as many alkaloids such as CAFFEINE and THEOPHYLLINE. Uric acid is the metabolic end product of purine metabolism.Heterocyclic Compounds with 4 or More Rings: A class of organic compounds containing four or more ring structures, one of which is made up of more than one kind of atom, usually carbon plus another atom. The heterocycle may be either aromatic or nonaromatic.Cladribine: An antineoplastic agent used in the treatment of lymphoproliferative diseases including hairy-cell leukemia.Azathioprine: An immunosuppressive agent used in combination with cyclophosphamide and hydroxychloroquine in the treatment of rheumatoid arthritis. According to the Fourth Annual Report on Carcinogens (NTP 85-002, 1985), this substance has been listed as a known carcinogen. (Merck Index, 11th ed)Acyclovir: A GUANOSINE analog that acts as an antimetabolite. Viruses are especially susceptible. Used especially against herpes.Amsacrine: An aminoacridine derivative that intercalates into DNA and is used as an antineoplastic agent.Anniversaries and Special Events: Occasions to commemorate an event or occasions designated for a specific purpose.G-Quadruplexes: Higher-order DNA and RNA structures formed from guanine-rich sequences. They are formed around a core of at least 2 stacked tetrads of hydrogen-bonded GUANINE bases. They can be formed from one two or four separate strands of DNA (or RNA) and can display a wide variety of topologies, which are a consequence of various combinations of strand direction, length, and sequence. (From Nucleic Acids Res. 2006;34(19):5402-15)History, 20th Century: Time period from 1901 through 2000 of the common era.History, 21st Century: Time period from 2001 through 2100 of the common era.Time Management: Planning and control of time to improve efficiency and effectiveness.History, 19th Century: Time period from 1801 through 1900 of the common era.History, 16th Century: Time period from 1501 through 1600 of the common era.History, 17th Century: Time period from 1601 through 1700 of the common era.History of DentistrySocieties, Scientific: Societies whose membership is limited to scientists.Aminacrine: A highly fluorescent anti-infective dye used clinically as a topical antiseptic and experimentally as a mutagen, due to its interaction with DNA. It is also used as an intracellular pH indicator.Aminoacridines: Acridines which are substituted in any position by one or more amino groups or substituted amino groups.Cardiotoxins: Agents that have a damaging effect on the HEART. Such damage can occur from ALKYLATING AGENTS; FREE RADICALS; or metabolites from OXIDATIVE STRESS and in some cases is countered by CARDIOTONIC AGENTS. Induction of LONG QT SYNDROME or TORSADES DE POINTES has been the reason for viewing some drugs as cardiotoxins.Antineoplastic Agents: Substances that inhibit or prevent the proliferation of NEOPLASMS.Databases, Chemical: Databases devoted to knowledge about specific chemicals.Antineoplastic Combined Chemotherapy Protocols: The use of two or more chemicals simultaneously or sequentially in the drug therapy of neoplasms. The drugs need not be in the same dosage form.Lymphoma: A general term for various neoplastic diseases of the lymphoid tissue.Databases, Pharmaceutical: Databases devoted to knowledge about PHARMACEUTICAL PRODUCTS.Giardiasis: An infection of the SMALL INTESTINE caused by the flagellated protozoan GIARDIA LAMBLIA. It is spread via contaminated food and water and by direct person-to-person contact.Pleural Effusion, Malignant: Presence of fluid in the PLEURAL CAVITY as a complication of malignant disease. Malignant pleural effusions often contain actual malignant cells.Pleural Effusion: Presence of fluid in the pleural cavity resulting from excessive transudation or exudation from the pleural surfaces. It is a sign of disease and not a diagnosis in itself.Chloroquine: The prototypical antimalarial agent with a mechanism that is not well understood. It has also been used to treat rheumatoid arthritis, systemic lupus erythematosus, and in the systemic therapy of amebic liver abscesses.Anthelmintics: Agents destructive to parasitic worms. They are used therapeutically in the treatment of HELMINTHIASIS in man and animal.Patient Care Bundles: Small sets of evidence-based interventions for a defined patient population and care setting.Oligonucleotides, Antisense: Short fragments of DNA or RNA that are used to alter the function of target RNAs or DNAs to which they hybridize.Oligonucleotide Array Sequence Analysis: Hybridization of a nucleic acid sample to a very large set of OLIGONUCLEOTIDE PROBES, which have been attached individually in columns and rows to a solid support, to determine a BASE SEQUENCE, or to detect variations in a gene sequence, GENE EXPRESSION, or for GENE MAPPING.Biotechnology: Body of knowledge related to the use of organisms, cells or cell-derived constituents for the purpose of developing products which are technically, scientifically and clinically useful. Alteration of biologic function at the molecular level (i.e., GENETIC ENGINEERING) is a central focus; laboratory methods used include TRANSFECTION and CLONING technologies, sequence and structure analysis algorithms, computer databases, and gene and protein structure function analysis and prediction.Oligonucleotide Probes: Synthetic or natural oligonucleotides used in hybridization studies in order to identify and study specific nucleic acid fragments, e.g., DNA segments near or within a specific gene locus or gene. The probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the probe include the radioisotope labels 32P and 125I and the chemical label biotin.

Uptake of acridinecarboxamide derivatives by L1210 cells. (1/161)

The uptake of six 9-aminoacridinecarboxamide derivatives by L1210 cells in relation to their lipophilicity and cytotoxic activity was studied. The amount of acridines taken up by cells was estimated by fluorimetric measurements. It was found that the uptake efficiency of this class of compounds by cells depends on the size of carboxamide residue as well as on position of the substituent. The increase of size of carboxamide chain resulted in the loss of capability of acridines to penetrate cell membrane. Cytotoxic effects of acridines were well correlated with the level of drugs accumulated by cells, whereas no clear correlation between uptake and lipophilicity was observed. It is concluded that uptake of 9-aminoacridinecarboxamides is the most important factor determining their antiproliferative activity.  (+info)

F0 complex of the Escherichia coli ATP synthase. Not all monomers of the subunit c oligomer are involved in F1 interaction. (2/161)

The antigenic determinants of mAbs against subunit c of the Escherichia coli ATP synthase were mapped by ELISA using overlapping synthetic heptapeptides. All epitopes recognized are located in the hydrophilic loop region and are as follows: 31-LGGKFLE-37, 35-FLEGAAR-41, 36-LEGAAR-41 and 36-LEGAARQ-42. Binding studies with membrane vesicles of different orientation revealed that all mAbs bind to everted membrane vesicles independent of the presence or absence of the F1 part. Although the hydrophilic region of subunit c and particularly the highly conserved residues A40, R41, Q42 and P43 are known to interact with subunits gamma and epsilon of the F1 part, the mAb molecules have no effect on the function of F0. Furthermore, it could be demonstrated that the F1 part and the mAb molecule(s) are bound simultaneously to the F0 complex suggesting that not all c subunits are involved in F1 interaction. From the results obtained, it can be concluded that this interaction is fixed, which means that subunits gamma and epsilon do not switch between the c subunits during catalysis and furthermore, a complete rotation of the subunit c oligomer modified with mAb(s) along the stator of the F1F0 complex, proposed to be composed of at least subunits b and delta, seems to be unlikely.  (+info)

Cellular uptake, cytotoxicity and DNA-binding studies of the novel imidazoacridinone antineoplastic agent C1311. (3/161)

C1311 is a novel therapeutic agent with potent activity against experimental colorectal cancer that has been selected for entry into clinical trial. The compound has previously been shown to have DNA-binding properties and to inhibit the catalytic activity of topoisomerase II. In this study, cellular uptake and mechanisms by which C1311 interacts with DNA and exerts cytotoxic effects in intact colon carcinoma cells were investigated. The HT29 colon cancer cell line was chosen to follow cellular distribution of C1311 over a time course of 24 h at drug concentrations that just inhibited cell proliferation by 50% or 100%. Nuclear uptake of C1311 and co-localization with lysosomal or mitochondrial dyes was examined by fluorescence microscopy and effects on these cellular compartments were determined by measurement of acid phosphatase levels, rhodamine 123 release or DNA-binding behaviour. The strength and mode of DNA binding was established by thermal melting stabilization, direct titration and viscometric studies of host duplex length. The onset of apoptosis was followed using a TUNEL assay and DNA-fragmentation to determine a causal relationship of cell death. Growth inhibition of HT29 cells by C1311 was concomitant with rapid drug accumulation in nuclei and in this context we showed that the compound binds to duplex DNA by intercalation, with likely A/T sequence-preferential binding. Drug uptake was also seen in lysosomes, leading to lysosomal rupture and a marked increase of acid phosphatase activity 8 h after exposure to C1311 concentrations that effect total growth inhibition. Moreover, at these concentrations lysosomal swelling and breakdown preceded apoptosis, which was not evident up to 24 h after exposure to drug. Thus, the lysosomotropic effect of C1311 appears to be a novel feature of this anticancer agent. As it is unlikely that C1311-induced DNA damage alone would be sufficient for cytotoxic activity, lysosomal rupture may be a critical component for therapeutic efficacy.  (+info)

Catalytic activities of mitochondrial ATP synthase in patients with mitochondrial DNA T8993G mutation in the ATPase 6 gene encoding subunit a. (4/161)

We investigated the biochemical phenotype of the mtDNA T8993G point mutation in the ATPase 6 gene, associated with neurogenic muscle weakness, ataxia, and retinitis pigmentosa (NARP), in three patients from two unrelated families. All three carried >80% mutant genome in platelets and were manifesting clinically various degrees of the NARP phenotype. Coupled submitochondrial particles prepared from platelets capable of succinate-sustained ATP synthesis were studied using very sensitive and rapid luminometric and fluorescence methods. A sharp decrease (>95%) in the succinate-sustained ATP synthesis rate of the particles was found, but both the ATP hydrolysis rate and ATP-driven proton translocation (when the protons flow from the matrix to the cytosol) were minimally affected. The T8993G mutation changes the highly conserved residue Leu(156) to Arg in the ATPase 6 subunit (subunit a). This subunit, together with subunit c, is thought to cooperatively catalyze proton translocation and rotate, one with respect to the other, during the catalytic cycle of the F(1)F(0) complex. Our results suggest that the T8993G mutation induces a structural defect in human F(1)F(0)-ATPase that causes a severe impairment of ATP synthesis. This is possibly due to a defect in either the vectorial proton transport from the cytosol to the mitochondrial matrix or the coupling of proton flow through F(0) to ATP synthesis in F(1). Whatever mechanism is involved, this leads to impaired ATP synthesis. On the other hand, ATP hydrolysis that involves proton flow from the matrix to the cytosol is essentially unaffected.  (+info)

Three-dimensional structures of Drosophila melanogaster acetylcholinesterase and of its complexes with two potent inhibitors. (5/161)

We have crystallized Drosophila melanogaster acetylcholinesterase and solved the structure of the native enzyme and of its complexes with two potent reversible inhibitors, 1,2,3,4-tetrahydro-N-(phenylmethyl)-9-acridinamine and 1,2,3,4-tetrahydro-N-(3-iodophenyl-methyl)-9-acridinamine--all three at 2.7 A resolution. The refined structure of D. melanogaster acetylcholinesterase is similar to that of vertebrate acetylcholinesterases, for example, human, mouse, and fish, in its overall fold, charge distribution, and deep active-site gorge, but some of the surface loops deviate by up to 8 A from their position in the vertebrate structures, and the C-terminal helix is shifted substantially. The active-site gorge of the insect enzyme is significantly narrower than that of Torpedo californica AChE, and its trajectory is shifted several angstroms. The volume of the lower part of the gorge of the insect enzyme is approximately 50% of that of the vertebrate enzyme. Upon binding of either of the two inhibitors, nine aromatic side chains within the active-site gorge change their conformation so as to interact with the inhibitors. Some differences in activity and specificity between the insect and vertebrate enzymes can be explained by comparison of their three-dimensional structures.  (+info)

A novel form of intercalation involving four DNA duplexes in an acridine-4-carboxamide complex of d(CGTACG)(2). (6/161)

The structures of the complexes formed between 9-amino-[N:-(2-dimethyl-amino)butyl]acridine-4-carboxamide and d(CG(5Br)UACG)(2) and d(CGTACG)(2) have been solved by X-ray crystallography using MAD phasing methodology and refined to a resolution of 1.6 A. The complexes crystallised in space group C222. An asymmetric unit in the brominated complex comprises two strands of DNA, one disordered drug molecule, two cobalt (II) ions and 19 water molecules (31 in the native complex). Asymmetric units in the native complex also contain a sodium ion. The structures exhibit novel features not previously observed in crystals of DNA/drug complexes. The DNA helices stack in continuous columns with their central 4 bp adopting a B-like motif. However, despite being a palindromic sequence, the terminal GC base pairs engage in quite different interactions. At one end of the duplex there is a CpG dinucleotide overlap modified by ligand intercalation and terminal cytosine exchange between symmetry-related duplexes. A novel intercalation complex is formed involving four DNA duplexes, four ligand molecules and two pairs of base tetrads. The other end of the DNA is frayed with the terminal guanine lying in the minor groove of the next duplex in the column. The structure is stabilised by guanine N7/cobalt (II) coordination. We discuss our findings with respect to the effects of packing forces on DNA crystal structure, and the potential effects of intercalating agents on biochemical processes involving DNA quadruplexes and strand exchanges. NDB accession numbers: DD0032 (brominated) and DD0033 (native).  (+info)

Acridine-a neglected antibacterial chromophore. (7/161)

The use of acridines as antimicrobial agents was first proposed by Ehrlich and Benda in 1912, and the first clinical use of these agents occurred in 1917. Many compounds containing the acridine chromophore were synthesized and tested, and the aminoacridines found wide use, both as antibacterial agents and as antimalarials, during World War II. The emergence of the penicillins eclipsed the acridines in antisepsis due to the greater therapeutic efficacies of the former. However, with the current massive increases in drug-resistant bacterial infection, new acridine derivatives may be of use. In addition, the topical utilization of aminoacridines in conjunction with directed low-power light offers bactericidal action at much lower doses.  (+info)

Protein-free parallel triple-stranded DNA complex formation. (8/161)

A 14 nt DNA sequence 5'-AGAATGTGGCAAAG-3' from the zinc finger repeat of the human KRAB zinc finger protein gene ZNF91 bearing the intercalator 2-methoxy,6-chloro,9-amino acridine (Acr) attached to the sugar-phosphate backbone in various positions has been shown to form a specific triple helix (triplex) with a 16 bp hairpin (intramolecular) or a two-stranded (intermolecular) duplex having the identical sequence in the same (parallel) orientation. Intramolecular targets with the identical sequence in the antiparallel orientation and a non-specific target sequence were tested as controls. Apparent binding constants for formation of the triplex were determined by quantitating electrophoretic band shifts. Binding of the single-stranded oligonucleotide probe sequence to the target led to an increase in the fluorescence anisotropy of acridine. The parallel orientation of the two identical sequence segments was confirmed by measurement of fluorescence resonance energy transfer between the acridine on the 5'-end of the probe strand as donor and BODIPY-Texas Red on the 3'-amino group of either strand of the target duplex as acceptor. There was full protection from OsO(4)-bipyridine modification of thymines in the probe strand of the triplex, in accordance with the presumed triplex formation, which excluded displacement of the homologous duplex strand by the probe-intercalator conjugate. The implications of these results for the existence of protein-independent parallel triplexes are discussed.  (+info)

*C13H10N2

The molecular formula C13H10N2 may refer to: Aminoacridines 2-Aminoacridine 3-Aminoacridine 4-Aminoacridine 9-Aminoacridine ...

*List of MeSH codes (D03)

... aminoacridines MeSH D03.494.046.250.150 --- acridine orange MeSH D03.494.046.250.177 --- acriflavine MeSH D03.494.046.250.200 ...
The free radical scavengers, dimethyl sulfoxide (Me2SO) and thiourea, were used to assess the role of free radicals in the production of intercalator-induced DNA breaks and cytotoxicity in mouse leukemia L1210 cells. Both agents decreased X-ray break production, and this decrease was comparable in magnitude to the degree of inhibition of X-ray-induced cell killing. By contrast, Me2SO increased the DNA breaks produced by the intercalators, Adriamycin, 5-iminodaunorubicin, and 4′-(9-acridinylamino)methanesulfon-m-anisidide. This was not due to an enhancement of Adriamycin or 4′-(9-acridinylamino)methanesulfon-m-anisidide uptake by Me2SO. Strand break production by intercalators was decreased by thiourea. This was not due to an inactivation of the intercalators or to a decrease of Adriamycin or 4′-(9-acridinylamino)methanesulfon-m-anisidide uptake by thiourea. Experiments using nucleoid sedimentation to assess the DNA linking number and domain size from cells treated with Me2SO and thiourea ...
Its also supposedly about conflicts of interest. Says the USA Today article: "Behind the modest rebellion is the belief that taking gifts from drug companies creates a conflict of interest for doctors. The argument: To accept handouts is to feel indebted, and doctors indebted to drug firms may not be prescribing medicines based solely on whats best for their patients." If thats the argument, why does the AMSA accept support from the anti-science based American Holistic Medical Association? The AMSA document "Successful Ways Past Retreats Found Moola" (which can be downloaded from the AMSA web sites section on Humanistic Medicine) says the American Holistic Medical Association gives out grants for humanistic medicine "retreats." Moreover, according to the document, in return for the grant "You have to contact the student rep and agree to give out AHMA (American Holistic Medical Association) materials at the retreat ...
Information about Amsa, Italy products database; lists of Amsa, Italy pharmaceutical drugs and health care production from Drugs-about.com
JBT has joined over 45 other companies and institutions as a Sustaining Partner of the AMSA. For more than 50 years, the AMSA has relied on support from partners to maintain its ability to provide community and professional development programs as well as opportunities for individuals in the field of meat science. AMSAs Sustaining Partner program recognizes the organizations and institutions that provide significant financial and logistical contributions for the products and services offered by the organization. read more ,, ...
The compound 4-(9-[4-[N-methyl-carboxamido]-5-methyl]acridinylamino)methanesulphon-m-a nisidide and acid addition salts thereof have antitumor properties.
A method for treating central nervous system or peripheral nervous system cholinergic deficit states such as Alzheimers disease in a mammal, said method comprising administering to said mammal an amount of a monoamine acridine derivative effective in the treatment of a cholinergic deficit state and for a time sufficient to achieve a suitable blood level to treat said cholinergic deficit state. The preferred monoamine acridine derivative is 1,2,3,4-tetrahydro-5-aminoacridine. A unit dosage pharmaceutical composition of matter comprising an effective amount of said monoamine acridine derivative sufficient to treat said cholinergic deficit state and a pharmaceutically acceptable inert carrier therefor is also described.
The DNA intercalator, 4′-(9-acridinylamino)methanesulfon-m-anisidide (m-AMSA) and the nonintercalator, etoposide (VP-16) produce topoisomerase II-mediated protein-linked DNA strand breaks. This function of topoisomerase II was investigated in relation to cell proliferation and cell cycle. Mouse fibroblasts NIH 3T3 and mouse leukemia L1210 cells stop proliferation when they reach a certain density. Nuclei were isolated from proliferative or quiescent cells and then treated with drug for 30 min. DNA modifications were assayed by alkaline elution. We found that the frequencies of m-AMSA- or VP-16-induced DNA-protein links were higher in nuclei from exponentially growing than in those from quiescent cells in both the 3T3 and the L1210 lines. Drug-induced protein-associated DNA breaks were also studied as a function of the cell cycle in 3T3 cells that had been arrested by contact inhibition in medium containing 1% calf serum and then stimulated to proliferate by replating at a lower cell density in ...
Sigma-Aldrich offers Aldrich-A38401, 9-Aminoacridine hydrochloride monohydrate for your research needs. Find product specific information including CAS, MSDS, protocols and references.
chemBlink provides information about CAS # 141946-28-3, 1,7-Bis(9-acridinyl)heptane, 1,7-Di(9-acridine)heptane, molecular formula: C33H30N2.
Nitracrine N-oxide | C18H20N4O3 | CID 159882 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity information, supplier lists, and more.
A faintly yellow solution with strong bluish violet fluorescence; used as a topical antiseptic and as a fluorescent stain in histology. SYN: 5 aminoacridine hydrochloride, 9 aminoacridine hydrochloride
The lacI gene of E. coli has been a highly useful target for studies of mutagenesis, particularly for analysis of the specificity (spectrum) of mutations generated under a variety of conditions and in various genetic backgrounds. The gene encodes the repressor of the lac operon, and lacI-defective mutants displaying constitutive expression of the operon are readily selected. DNA sequencing of the lacI mutants has often been confined to the N-terminal region of the protein, as it presents a conveniently short target with a high density of detectably mutable sites ...
Interview with Birmingham Fellow, Pola Goldberg Oppenheimer: Pola leads a research group focusing on Advanced Materials, Structures and Applications (AMSA) in the School of Chemical Engineering. This summer, she will address listeners at the 2014 Soapbox Science event at Londons Southbank having been selected as a leading UK female scientist.
The synthesis of a new bifunctional compound in which two aminoacridine chromophores are linked by the bicyclic depsipeptidic backbone of des-N-tetramethylTriostin A is described. The molecule, bis-[(9-acridinyl)-D-seryl-L-alanyl-L-cysteinyl-L-valine] dilactone disulphide, structurally analogous to the antibiotic anti-tumour drug Triostin A, is shown to possess a high affinity to DNA and to act as a bis-intercalator on the basis of spectroscopic, viscosimetric and thermal-denaturation studies. This model constitutes the first attempt of a synergic association between a peptidic moiety that mimics a naturally occurring drug and aminoacridine, the two parts themselves each exhibiting a high affinity for the DNA target. ...
article{dd800d7b-fd6e-4ee6-9ef4-ae39c88634e1, abstract = {Biochemical and histochemical studies have demonstrated a widespread deficit in the activity of acetylcholinesterase (AChE) in the brains of patients with Alzheimers disease (DAT). Multiple disturbances in several transmitter systems have been found. The most consistent neurochemical changes in DAT are reductions in the cholinergic system. The major pharmacological approach today in DAT is based on the cholinergic theory assuming that acetylcholine has a major cortical impact on cognitive processes. Tetrahydroaminoacridine (THA, tacrine) is a centrally active reversible acetylcholinesterase inhibitor. A large number of trials have been performed in patients with DAT. This article was to evaluate whether THA treatment induced neuropeptide alteration in DAT before and after 1 year on oral THA treatment.}, author = {Minthon, Lennart and Edvinsson, Lars and Gustafson, Lars}, issn = {1741-2684}, language = {eng}, number = {6}, pages = ...
Alzheimers disease affects about 4 million people in the United States and is characterized by a degradation of cholinergic nerves in the cerebral cortex and hippocampus leading to a decrease in cholinergic transmission (Owens, 1993). Drug therapy to increase cholinergic transmission has been one strategy used to combat the symptoms of Alzheimers disease (Starr, 1992; Volger, 1991). Tetrahydroaminoacridine (also known as THA, tacrine, and Cognex) is the first agent approved by the Food and Drug Administration for treatment of Alzheimers disease. Tacrine acts as an acetylcholinesterase inhibitor to block the degradation of acetylcholine in the neurons of cerebral cortex thereby increasing cholinergic transmission (Wu and Yang, 1989; Hunter et al., 1989). Preclinical trials showed that many Alzheimers patients had a significant improvement in symptoms when tacrine (2-3 mg/kg/day) was administered on a daily basis (Farlow et al., 1992; Gamzu et al., 1990;Summers et al., 1981). However, ...
Since the properties of small molecules can change upon conjugation to proteins, we investigated the binding of CB[8] to TMV-6 following the confirmation of the TMV-6 conjugate. Since the solutions of TMV-6 at concentrations required for ITC characterization were subject to viscosity and adhesion issues, a fluorescence titration was used instead to determine a dissociation constant. In this case, neither TMV-6 nor CB[8] contain fluorophores, so a competitive binding strategy incorporating a fluorophore was implemented. Acridine-3,6-diamine, or proflavin (PF), is an acridine derivative that is fluorescent as a free molecule in aqueous solutions, but its fluorescence becomes quenched68,69 upon binding inside the cavity of CB[8]. A fluorescence titration of increasing concentrations of TMV-6 (0-20 μM relative to TEMPO) titrated into solutions with a constant concentration of the CB[8]⊃PF (0.2 μM) complex was performed. Upon the addition of increasing concentrations of TMV-6, the observed ...
Aminoacridine derivative that is a potent intercalating antineoplastic agent. It is effective in the treatment of acute leukemias and malignant lymphomas, but has poor activity in the treatment of solid tumors. It is frequently used in combination with other antineoplastic agents in chemotherapy protocols. It produces consistent but acceptable myelosuppression and cardiotoxic effects. [PubChem]
Abdul Mamsa, MD is a Neurologist at 820 W Oak St, Kissimmee, FL 34741. Wellness.com provides reviews, contact information, driving directions and the phone number for Abdul Mamsa, MD in Kissimmee, FL.
Figure 1 Quiz of the Week A 35-year-old male presents to his family physician with progressive heel pain. He denies any recent trauma and mentions the
9-Fluoro-7-methylbenzo[c]acridine/ACM482417 can be provided in Alfa Chemistry. We are dedicated to provide our customers the best products and services.
0145] A wide variety of fluorescent molecules can be utilized in the present invention including small molecules, fluorescent proteins and quantum dots. Useful fluorescent molecules (fluorophores) include, but are not limited to: 1,5 IAEDANS; 1,8-ANS; 4-Methylumbelliferone; 5-carboxy-2,7-dichlorofluorescein; 5-Carboxyfluorescein (5-FAM); 5-Carboxynapthofluorescein; 5-Carboxytetramethylrhodamine (5-TAMRA); 5-FAM (5-Carboxyfluorescein); 5-HAT (Hydroxy Tryptamine); 5-Hydroxy Tryptamine (HAT); 5-ROX (carboxy-X-rhodamine); 5-TAMRA (5-Carboxytetramethylrhodamine); 6-Carboxyrhodamine 6G; 6-CR 6G; 6-JOE; 7-Amino-4-methylcoumarin; 7-Aminoactinomycin D (7-AAD); 7-Hydroxy-4-methylcoumarin; 9-Amino-6-chloro-2-methoxyacridine; ABQ; Acid Fuchsin; ACMA (9-Amino-6-chloro-2-methoxyacridine); Acridine Orange; Acridine Red; Acridine Yellow; Acriflavin; Acriflavin Feulgen SITSA; Aequorin (Photoprotein); AFPs--AutoFluorescent Protein--(Quantum Biotechnologies); Alexa Fluor 350®; Alexa Fluor 430®; Alexa Fluor ...
In 1986, a lead article (1) in New England Journal of Medicine reported the results of a small crossover trial of oral tetrahydroaminoacridine (THA) in patients with Alzheimer disease aged 40 years and older. The accompanying editorial explained how the improvement seen in most patients validated the hypothesis that Alzheimer disease is characterized by a cholinergic deficit. The paper and editorial helped create a general expectation and, for the public, hope that cholinergic enhancement therapy with cholinesterase inhibitors would help reverse the cognitive and behavioral impairments of Alzheimer disease, just as levodopa had revolutionized treatment of another neurodegenerative disease, Parkinson disease. The 1986 trial provided what was probably the most optimistic setting in which to show the efficacy of THA (now called tacrine). The systematic review by López Arrieta and Rodriguez Artalejo confirms what many clinicians have known for years. Only a few patients benefit from tacrine, and ...
A human breast cancer cell line (MCF7/WT) was selected for resistance to etoposide (VP-16) by stepwise exposure to 2-fold increasing concentrations of this agent. The resulting cell line (MCF7/VP) was 28-, 21-, and 9-fold resistant to VP-16, VM-26, and doxorubicin, respectively. MCF7/VP cells also exhibited low-level cross-resistance to 4′-(9-acridinylamino)-methanesulfon-m-anisidide, mitoxantrone, and vincristine and no cross-resistance to genistein and camptothecin. Furthermore, these cells were collaterally sensitive to the alkylating agents melphalan and chlorambucil. DNA topoisomerase II levels were similar in both wild-type MCF7/WT and drug-resistant MCF7/VP cells. In contrast, topoisomerase II from MCF7/VP cells appeared to be 7-fold less sensitive to drug-induced cleavable complex formation in whole cells and 3-fold less sensitive in nuclear extracts than topoisomerase II from MCF7/WT cells. Although this suggested that the resistant cells may contain a qualitatively altered ...
A novel method to determine of azaarenes in refined and cold-pressed vegetable oils and animal fats is reported. The method may be used to determine eight most important acridine derivatives (benz[a]acridine, dibenz[a,i]acridine, benz[c]acridine, dibenz[a,j]acridine, 7,9-dimethylbenz[c]acridine, dibenz[a,h]acridine, dibenz[a,c]acridine, dibenz[c,h]acridine) at a high sensitivity (LOQ in the 2-25 ng kg(-1) range), high ...
An acridine derivative formerly widely used as an antimalarial but superseded by chloroquine in recent years. It has also been used as an anthelmintic and in the treatment of giardiasis and malignant effusions. It is used in cell biological experiments as an inhibitor of phospholipase A2.
The genome of the food-borne pathogen Campylobacter jejuni contains multiple highly mutable sites, or contingency loci. It has been suggested that standing variation at these loci is a mechanism for rapid adaptation to a ...
The effect of three accelerators of hemolysis-acetylphenylhydrazine, 9-aminoacridine, and phenothiazone-upon protein and lipid monolayers has been examined. These compounds, in low concentration, cause marked expansion of monolayers of plasma albumin, but have no apparent effect upon cholesterol films. It is suggested that these accelerators may affect the protein component of the erythrocyte membrane, thus enhancing the action of hemolytic agents.. ...
10-Bromonaphth[2,3-c]acridine-5,8,14(13H)-trione/ACM52636596 can be provided in Alfa Chemistry. We are dedicated to provide our customers the best products and services.
Congratulations! You have found the Acridine Forum on Forum Jar. This forum is a place where people who are interested in Acridine come together and discuss about Acridine. Please use the message board below to post anything related to Acridine . If you are interested in other similar forums, please check out the Related Forums section on the right. If you like this forum, please dont forget to tell your friends about Forum Jar ...
Hey AMSA! Veritas Preps team of medical school admissions consultants answer tons of your admission questions including topics such as how to pay for medical school and what a day in the life of a first year med student is … Continue reading →. ...
Figure 1 The structure of the title complex, with displacement ellipsoids drawn at the 50% probability level for non-H atoms. Unlabelled atoms are related to the reference atoms by the (1 − x, 1 − y, −z) symmetry transformation ...
It has been clear for much time that cigarette smoking is harmful to the health of smokers and non-smokers alike. Listed below is an incomplete list of the harmful effects of both second-hand and mainstream smoke. Also below is a link to an informative and helpful website from the The New York City Department of Health and Men. Although most of us know that smoking increases ones risk for a variety of cancers, most notably lung cancer, many are unaware of the other harmful effects of smoking. As we go through our studies, we will identify those not-so-obvious effects of smoking. This list is under construction and is by no means exhaustive!. Specific references for some of the conditions can be found below. Otherwise, the information can be readily found in Robbins Pathologic Basis of Disease, 7th Ed.. ...
The American Association of Meat Processors (AAMP) and the American Meat Science Association (AMSA) are excited to announce that the University of Missouri will be hosting a PORK 101 course January 13-15, 2016 in Columbia, Missouri. PORK 101 is hosted by AMSA in cooperation with the National Pork Board and is sponsored by Elanco Animal ...
Purpose: To inspire a community of future physicians through education an advocacy. Faculty Advisor: Steve Karr Email Address: [email protected] Student President: Catherine Duke Email Address: [email protected] ...
Hey AMSA! Just passing along a message from our Kaplan representative, Molly Russell: Because you are a valued partner, we would like to take this opportunity to give you a sneak peek at our upcoming promotion, which will begin on … Continue reading →. ...
acridine 260-94-6 MSDS report, acridine MSDS safety technical specifications search, acridine safety information specifications ect.
Acridine Orange: A cationic cytochemical stain specific for cell nuclei, especially DNA. It is used as a supravital stain and in fluorescence cytochemistry. It may cause mutations in microorganisms.
DNA intercalating dye. A grade of acridine orange of exceptionally high purity, suitable for quantitative work. Free of inorganic salts.
Define acridine: a colorless crystalline compound C13H9N occurring in coal tar and important as the parent compound of dyes and pharmaceuticals
... is a prescription Alzheimers disease medication. This page on the eMedTV site describes how tacrine works to slow the worsening of Alzheimers symptoms, explains when and how to take the drug, and lists possible side effects that may occur.
If you dont have a website, then now is the time to get one. Even if you dont need traffic from Google, you want potential customers to be able to look you up. For example, if I need the services of a landscaper then I am going to Google landscapers in my area. You better believe that I am more apt to call one that has his or her own website than one that doesnt. Yes, I can go pick up the Yellow Pages and do a search, but Im too lazy. I imagine many others are also turning to the Internet instead of the Yellow Pages ...
We, China 3,4-Diaminopyridine Manufacturers, China 3,4-Diaminopyridine Suppliers, provide quality 3,4-Diaminopyridine product and the products related with China 3,4-Diaminopyridine - nwchemical
88797-46-0 - RGPWZNMVNPBMGP-UHFFFAOYSA-N - Benz(c)oxireno(a)acridine-11-methanol, 1a,11b-dihydro- - Similar structures search, synonyms, formulas, resource links, and other chemical information.
Methods are disclosed for inhibiting the infectivity of HIV-1 in human cells. The methods comprise contacting human cells infected with HIV-1, with certain quinolinyl and acridinyl derivatives, including amodiaquin, chloroquine, hydroxychloroquine, primoquine, quinacrine and compounds having the formula: ##STR1## wherein R1 and R2 are each hydrogen, or join to form a cyclic structure of the formula: ##STR2## and R3 and R4, same or different, are hydrogen, C1 -C8 lower alkyl or hydroxy substituted C1 -C8 lower alkyl, and the pharmaceutically acceptable salts thereof.
Looking for online definition of acridine in the Medical Dictionary? acridine explanation free. What is acridine? Meaning of acridine medical term. What does acridine mean?
Acridine orange is a dye that intercalates or binds with the nucleic acid ( either DNA or RNA) present in organisms and fluoresce to emit various colors that help in differentiation of cellular organells. This binding is the result the electrostatic interactions of acridine molecule between the nucleic acid base pairs. Acridine Orange (AO), due to its metachromatic properties, is commonly used in fluorescence microscopy and flow cytometry analysis of cellular physiology and cell cycle status, including the fluorescent microscopic examination of microorganisms. ...
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Sigma-Aldrich offers Sigma-A9231, Acridine Orange solution for your research needs. Find product specific information including CAS, MSDS, protocols and references.
Potential side effects of tacrine include insomnia, loss of appetite, and fatigue. This section of the eMedTV library lists other tacrine side effects, including common, rare, and potentially serious side effects that have been reported with the drug.
Looking for online definition of 3,4-diaminopyridine in the Medical Dictionary? 3,4-diaminopyridine explanation free. What is 3,4-diaminopyridine? Meaning of 3,4-diaminopyridine medical term. What does 3,4-diaminopyridine mean?
Quinacrine dihydrochloride is the dihydrochloride salt of the 9-aminoacridine derivative quinacrine with potential antineoplastic and antiparasitic activities. Quinacrine may inhibit the transcription and activity of both basal and inducible nuclear factor-kappaB (NF-kappaB), which may result in the induction of tumor suppressor p53 transcription, the restoration of p53-dependent apoptotic pathways, and tumor cell apoptosis. Continuous NF-kappaB signaling, present in many tumors and in chronic inflammatory processes, promotes the expression of antiapoptotic proteins and cytokines while downregulating the expression of proapoptotic proteins, such as p53. Check for active clinical trials or closed clinical trials using this agent. (NCI Thesaurus) .
Upcoming Medical School Visits: We have decided that instead of attempting to squeeze another medical school trip into this semester, we will be going on *three * next semester. If you want to go on any of these, you MUST attend a stadium clean-up before the end of the semester. We have two left--this upcoming Sunday (after the Alabama game) and the Sunday after the Ole Miss game. Both clean-ups will begin at 6am. (Remember: one stadium clean-up equals one medschool visit). Congrats to our new chapter development officer, Sam Sullivan!. Next Meeting: November 19th! See you at 7pm in the Taylor Auditorium of McCool Hall.... Dont forget about making some money off those old MCAT materials. Contact Brooke Harris at [email protected] if you are interested. Registration for the January offerings of the MCAT is open. Visit www.aamc.org/mcat. ...
The amount of muscle and the thickness of the skin and ligaments depend upon a persons constitution. People with a vata prakruti have minimal amounts of muscle and thin skin and ligaments even when healthy and balanced. Those with a pitta nature have moderate muscular formation along with a moderate thickness of the skin and ligaments. Individuals with a kapha nature have larger muscle mass with thicker skin and ligaments. Regardless of the dosha, the tissues are healthy if they are consistent in formation with the doshic balance of the individual and are tone and supple.. Vitiation of kapha dosha in the mamsavaha srota (channel that carries posaka rakta dhatu) results in low mamsagni. This results in excessive mamsa dhatu formation but the tissue formed is hard and inflexible. In addition, the upadhatus (secondary tissues) are similarly affected. Thus, the skin and ligaments of the body become thicker, harder, and tighter. Psychologically, self-confidence is quiet and strong but the motivation ...
Acridine-9-carboxylic acid hydrate 5336-90-3 Precursor and Downstream products, Acridine-9-carboxylic acid hydrate Precursor products, Acridine-9-carboxylic acid hydrate Downstream products ect.
The term [Unfavorable Study] as used is exemplified by Molloy et al.(28) They concluded, "THA has no clinically important benefits in Alzheimers disease and is associated with appreciable toxic effects." The flaws listed in Table 1 show how this erroneous conclusion was reached. The dose of THA (tacrine hydrochloride) was beyond not adequate. The dose [Adequate Tacrine] was wrong. The authors used apparently a single daily dose of 50, 75, or 100 mg for two weeks. In our studies the maximum dose was 50 mg q 6 hours (200 mg/day). Tacrine hydrochloride is not a once a day drug and single doses above 50 mg per day would result in higher toxicity with poorer response to endpoints desired. Many of the subjects "tested" in this study were no doubt toxic with tacrine at the time of testing. In the category of [Personalized Dose], Molloy et al. went to great lengths to find optimal doses for subject. They then disregarded their own findings. They randomly assigned the patients to "eight possible orders ...
Up to 15 patients over the age of 18 years with a diagnosis of LEMS are eligible to enroll if they are medically stable. They may receive 3,4 diaminopyridine in addition to other treatments and standard of care investigations for LEMS under supervision of the primary investigator. Safety laboratory studies and EKGs will be obtained.. The study has been approved by the University of Pittsburgh IRB. There is a local Data-Safety Monitoring Board.. The investigator has a hold on enrolling new subjects. ...
In deciding to use a medicine, the risks of taking the medicine must be weighed against the good it will do. This is a decision you and your doctor will make. For this medicine, the following should be considered: Allergies Tell your doctor if you have ever had any unusual or allergic reaction to this medicine or any other medicines. Also tell your health care professional if you have any other types of allergies, such as to foods, dyes, preservatives, or animals. For non-prescription products, read the label or package ingredients carefully. Children Studies on this medicine have been done only in adult patients, and there is no specific information comparing use of amsacrine in children with use in other age groups. Older adults Many medicines have not been studied specifically in older people. Therefore, it may not be known whether they work exactly the same way they do in younger adults or if they cause different side effects or problems in older people. There is no specific information ...
This thesis describes the synthesis and radiohalogenation of compounds of potential use for tumor targeting.. The first section describes the synthesis and radioiodination of DNA intercalating compounds. The compounds are derivatives of 9-aminoacridine, and the anthracyclins daunorubicin and doxorubicin. The precursor compounds were labeled with 125I (T1/2 = 60 days), which is an Auger emitting nuclide. 125I decaying in the close vicinity of DNA is known to have a much higher cell killing effect than 125I decaying in the cytoplasm and some of the labeled compounds prepared in this thesis are currently being tested for use in targeted radionuclide therapy for cancer.. The second section describes the radiobromination of closo-carboranes by subjecting the corresponding iodinated compounds to palladium-catalyzed halogen exchange using [76Br]bromide. The 76Br isotope (T1/2 = 16.2 h) is a positron emitting nuclide that is suitable for PET studies. Via the halogen exchange reaction good to excellent ...
http://twitter.com/AMSA_News/status/420664900914925568 It seems my forecast worked out well. From RT news: A Russian-built ship stranded in the Antarctic ice has started moving away from the ice fields after a change of wind cleared its path. A Chinese icebreaker, which was caught herself on the way to rescue the vessel, has already reached clear waters. ...…
BioAssay record AID 693243 submitted by ChEMBL: Induction of apoptosis in human MGC803 cells assessed as pyknosis at 5 uM after 12 to 48 hrs by acridine orange/ethidium bromide staining.
36762-16-0 - XQLYYXFSXAIGJZ-UHFFFAOYSA-N - Acridine, 9-(2-chlorophenyl)- - Similar structures search, synonyms, formulas, resource links, and other chemical information.
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BACKGROUND Quantitative analysis of ventricular fibrillation (VF), such as amplitude spectral area (AMSA), predicts shock outcomes. However, there is no uniform definition of shock/cardiopulmonary resuscitation (CPR) success in out-of-hospital cardiac arrest (OHCA). The objective of this study is to investigate post-shock rhythm variations and the impact of shock/CPR success definition on the predictability of AMSA. METHODS A total of 554 shocks from 257 OHCA patients with VF as initial rhythm were analyzed. Post-shock rhythms were analyzed every 5s up to 120s and annotated as VF, asystole (AS) and organized rhythm (OR) at serial time intervals. Three shock/CPR success definitions were used to evaluate the predictability of AMSA: (1) termination of VF (ToVF); (2) return of organized electrical activity (ROEA); (3) return of potentially perfusing rhythm (RPPR). RESULTS Rhythm changes occurred after 54.5% (N=302) of shocks and 85.8% (N=259) of them occurred within 60s after shock delivery. The
To gain more detailed insight into the nature and mechanisms of spontaneous mutations, we undertook a DNA sequence analysis of a large collection of spontaneous mutations in the N-terminal region of the Escherichia coli lacI gene. This region of circa 210 base pairs is the target for dominant lacI mutations (i-d) and is suitable for studies of mutational specificity since it contains a relatively high density of detectable mutable sites. Among 414 independent i-d mutants, 70.8% were base substitutions, 17.2% deletions, 7.7% additions and 4.3% single-base frameshifts. The base substitutions were both transitions (60%) and transversions (40%), the largest single group being G.C----A.T (47% of base substitutions). All four transversions were observed. Among the 71 deletions, a hotspot (37 mutants) was present: an 87-bp deletion presumably directed by an 8-bp repeated sequence at its endpoints. The remaining 34 deletions were distributed among 29 different mutations, either flanked (13/34) or not ...
We do routinely use acridine orange using FACScan. A 20 min rinse with 10 times water-diluted Chlorox bleach is adequate to clean the instrument. Zbigniew Darzynkiewicz -----Original Message----- From: Karen Creswell [mailto:creswelk at georgetown.edu] Sent: Friday, June 04, 2004 1:17 PM To: cyto-inbox Subject: Acridine Orange Does anyone routinely run acridine orange through their FACScan/FACSort? I would like suggestions for cleaning the instrument afterwards. Thanks for your help, Karen -- Karen Creswell, Ph.D. Director, Flow Cytometry/Cell Sorting Shared Resource Lombardi Cancer Center Georgetown University Medical Center Research Bldg W304 Box 571469 Washington, DC 20057-1469 202-687-2233 creswelk at georgetown.edu ...
Synonyms for acridine dye in Free Thesaurus. Antonyms for acridine dye. 30 synonyms for dye: colour, stain, tint, shade, tinge, pigment, tincture, colour-wash, colouring, colour, pigment, wash, stain, tint, tinge, colorant, color. What are synonyms for acridine dye?
Police inspector Amsa gives a radio interview about domestic violence. After Bibi Yaji was beaten to death by her husband, the villagers of Lambu organize a rally to protest again domestic violence.
... ,Application: Acridine orange has been used as a fluorescent stain for nucleic acids in agarose and polyacrylamide gels. Acridine orange at a concentration of 120 μM will detect purified DNA in gels with a sensitivity of 25-50 ng per band. It has also been used extensively for cell staining of,biological,biology supply,biology supplies,biology product

Hypoxia-selective antitumor agents. 4. Relationships between structure, physicochemical properties, and hypoxia-selective...Hypoxia-selective antitumor agents. 4. Relationships between structure, physicochemical properties, and hypoxia-selective...

Aminoacridines. *Animals. *Antineoplastic Agents (chemical synthesis, pharmacology) *Cell Hypoxia (drug effects) *Cell Line ...
more infohttp://www.curehunter.com/public/pubmed2329552.do

Advanced Search Results - Public Health Image Library(PHIL)Advanced Search Results - Public Health Image Library(PHIL)

Categories: Aminoacridines Image Types: Photo, Illustrations, Video, Color, Black&White, PublicDomain, CopyrightRestricted 1 ...
more infohttps://phil.cdc.gov/AdvancedSearchResults.aspx?Search=Aminoacridines&parentid=1846&catid=2590

Tacrine
      - Cognex
     Summary Report | CureHunterTacrine - Cognex Summary Report | CureHunter

Aminoacridines: 3*Tacrine: 566*velnacrine: 22. *1,7-N-heptylene-bis-9,9-amino-1,2,3,4-tetrahydroacridine: 16 ...
more infohttp://www.curehunter.com/public/keywordSummaryD013619-Tacrine-Cognex.do

3,6-diaminoacridine (CHEBI:8452)3,6-diaminoacridine (CHEBI:8452)

... is a aminoacridines (CHEBI:51803) 3,6-diaminoacridine (CHEBI:8452) is conjugate base of 3,6- ...
more infohttps://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI:45272

Patent US4257774 - Intercalation inhibition assay for compounds that interact with DNA or RNA - Google PatentsPatent US4257774 - Intercalation inhibition assay for compounds that interact with DNA or RNA - Google Patents

Ellerton, et al., "The Interaction of DNA with Aminoacridines and Aminobenzacridines", Chem. Absts., vol. 89, No. 13 (1978), p ...
more infohttp://www.google.com.au/patents/US4257774

Molecules | Free Full-Text | Synthesis, Molecular Structure and Reactivity of 5-Methylidene-1,2,3,5-tetrahydroimidazo[2,1-b...Molecules | Free Full-Text | Synthesis, Molecular Structure and Reactivity of 5-Methylidene-1,2,3,5-tetrahydroimidazo[2,1-b...

Structure-Activity Relationships for the 9-(Pyridin-2-yl)- aminoacridines. Previous Article in Journal / Special Issue. ...
more infohttp://www.mdpi.com/1420-3049/9/3/86

C13H10N2 - WikipediaC13H10N2 - Wikipedia

The molecular formula C13H10N2 may refer to: Aminoacridines 2-Aminoacridine 3-Aminoacridine 4-Aminoacridine 9-Aminoacridine ...
more infohttps://en.wikipedia.org/wiki/C13H10N2

US2841587A - Separation of mixtures of deserpidine  - Google PatentsUS2841587A - Separation of mixtures of deserpidine - Google Patents

Basic nitroderivatives of 9-aminoacridines and process for preparing the same Dudley et al. 1927. The constitution and ...
more infohttps://patents.google.com/patent/US2841587A/en

Welcome to the DeRisi LabWelcome to the DeRisi Lab

Parallel synthesis of 9-aminoacridines and their evaluation against chloroquine-resistant Plasmodium falciparum.. Anderson MO, ... Parallel synthesis of 9-aminoacridines and evaluation against chloroquine-resistant falciparum. Anderson MO, Sherrill J, Madrid ...
more infohttp://derisilab.ucsf.edu/index.php?page=publications

Chemical Relaxation Kinetic Stu dies of E. coli RN A Polymerase Bin ding to Poly [ d( A- T)] Using Ethi dium Bromi de as a...Chemical Relaxation Kinetic Stu dies of E. coli RN A Polymerase Bin ding to Poly [ d( A- T)] Using Ethi dium Bromi de as a...

BLAKE, A., PEACOCKE, A.R.: The interaction of aminoacridines with nucleic acids. Biopolymers 6, 1225-1253 (1968)PubMedCrossRef ...
more infohttps://rd.springer.com/chapter/10.1007/978-3-642-81117-3_7

Cytologie des Magens | Springer for Research & DevelopmentCytologie des Magens | Springer for Research & Development

Ackerman, N. B., Haldorsen, D. K., Tendick, F. H., Elslager, E. F.: Preparation and screening of aminoacridines for induction ...
more infohttps://rd.springer.com/chapter/10.1007%2F978-3-642-65881-5_8

The Effect of a New Compound (Ravinol Salts) and Yemeni Honey on ... | Publish your masters thesis, bachelors thesis, essay...The Effect of a New Compound (Ravinol Salts) and Yemeni Honey on ... | Publish your master's thesis, bachelor's thesis, essay...

In addition, the topical utilization of aminoacridines in conjunction with directed low-power light offers microbicidal action ... and the aminoacridines found wide use, both as antibacterial agents and as antimalarials, during World War II. The emergence of ...
more infohttps://www.grin.com/document/450259

RCSB PDB - PRL Ligand Summary PageRCSB PDB - PRL Ligand Summary Page

Aminoacridines. *Anti-Infective Agents. *Anti-Infective Agents, Local. *Chemical Actions and Uses ...
more infohttps://www3.rcsb.org/ligand/PRL

List of MeSH codes (D03) - WikipediaList of MeSH codes (D03) - Wikipedia

... aminoacridines MeSH D03.494.046.250.150 --- acridine orange MeSH D03.494.046.250.177 --- acriflavine MeSH D03.494.046.250.200 ...
more infohttps://en.wikipedia.org/wiki/List_of_MeSH_codes_(D03)

In s S. Albuquerque - Cita  es do Google Acad micoIn s S. Albuquerque - Cita es do Google Acad mico

In vitro efficiency of 9-(N-cinnamoylbutyl) aminoacridines against blood-and liver-stage malaria parasites. B P rez, C Teixeira ...
more infohttps://scholar.google.pt/citations?user=vgqKsaQAAAAJ&hl=pt-PT&oe=ASCII

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Induction of DNA-protein crosslinks by antitumor 1-nitro-9-aminoacridines in L1210 leukemia cells. Biochem Pharmacol 1989;38: ...
more infohttp://mct.aacrjournals.org/content/3/11/1403

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Quinacrine displaced 97.2% of DNA-bound methyl green at rates higher than those caused by aminoacridines which did not possess ...
more infohttp://molpharm.aspetjournals.org/content/10/4/686

previouspres.htmlpreviouspres.html

"Synthesis and Antitumor Activity of Novel Bis-9-Aminoacridines," G.D. Jaycox, G.W. Gribble, and M.P. Hacker, Poster presented ... "Conformationally Restricted Bis(9-aminoacridines): DNA Binding and Antitumor Properties," G.D. Jaycox, M.D. Mosher, and G.W. ... "Conformationally Restricted Bis(9-aminoacridines): DNA Binding and Antitumor Properties," G.D. Jaycox, G.W. Gribble, M. Cory, T ...
more infohttp://www.dartmouth.edu/~gwgchem/previouspres.html

Patent US6689563 - System and methods for sequencing by hybridization - Google PatentsPatent US6689563 - System and methods for sequencing by hybridization - Google Patents

9-aminoacridines, p,p′-diaminobenzophenone imines, anthracenes, oxacarbocyanine, merocyanine, 3-aminoequilenin, perylene, bis- ...
more infohttp://www.google.com/patents/US6689563?dq=5311516

Patent US7179423 - Microfluidic system including a virtual wall fluid interface port for ... - Google PatentsPatent US7179423 - Microfluidic system including a virtual wall fluid interface port for ... - Google Patents

9-aminoacridines, p,p′-diaminobenzophenone imines, anthracenes, oxacarbocyanine, merocyanine, 3-aminoequilenin, perylene, bis- ...
more infohttp://www.google.com.au/patents/US7179423

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... the aminoacridines (exemplified by amsacrine), and the anthracyclines (exemplified by doxorubicin, daunorubicin, and idarubicin ...
more infohttp://cancerres.aacrjournals.org/content/65/16/7470

9-aminoacridine Inhibition of HIV-1 Tat Dependent Transcription | Virology Journal | Full Text9-aminoacridine Inhibition of HIV-1 Tat Dependent Transcription | Virology Journal | Full Text

Sebestik J, Hlavacek J, Stibor I: A role of the 9-aminoacridines and their conjugates in a life science. Curr Protein Pept Sci ... The inhibition demonstrated by 9AA is specific to this class of compounds, as other amino acridines did not display activity to ...
more infohttps://virologyj.biomedcentral.com/articles/10.1186/1743-422X-6-114

US6753160B2 - Method of using metaloporphyrins for treatment of arteriosclerotic lesions 
        - Google PatentsUS6753160B2 - Method of using metaloporphyrins for treatment of arteriosclerotic lesions - Google Patents

Abstract, Schwartz, G., et al., "Selected Amino Acridines as Fluorescent Probes in Cytochemistry in General and in the ...
more infohttps://patents.google.com/patent/US6753160B2/en

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