AmidohydrolasesUreohydrolasesAllantoin: A urea hydantoin that is found in URINE and PLANTS and is used in dermatological preparations.Databases, Protein: Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.Internet: A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.User-Computer Interface: The portion of an interactive computer program that issues messages to and receives commands from a user.Software: Sequential operating programs and data which instruct the functioning of a digital computer.Proteins: Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.Polyunsaturated Alkamides: Amides composed of unsaturated aliphatic FATTY ACIDS linked with AMINES by an amide bond. They are most prominent in ASTERACEAE; PIPERACEAE; and RUTACEAE; and also found in ARISTOLOCHIACEAE; BRASSICACEAE; CONVOLVULACEAE; EUPHORBIACEAE; MENISPERMACEAE; POACEAE; and SOLANACEAE. They are recognized by their pungent taste and for causing numbing and salivation.Endocannabinoids: Fatty acid derivatives that have specificity for CANNABINOID RECEPTORS. They are structurally distinct from CANNABINOIDS and were originally discovered as a group of endogenous CANNABINOID RECEPTOR AGONISTS.Receptors, Cannabinoid: A class of G-protein-coupled receptors that are specific for CANNABINOIDS such as those derived from CANNABIS. They also bind a structurally distinct class of endogenous factors referred to as ENDOCANNABINOIDS. The receptor class may play a role in modulating the release of signaling molecules such as NEUROTRANSMITTERS and CYTOKINES.Arachidonic AcidsPhenylmethylsulfonyl Fluoride: An enzyme inhibitor that inactivates IRC-50 arvin, subtilisin, and the fatty acid synthetase complex.Cannabinoids: Compounds having the cannabinoid structure. They were originally extracted from Cannabis sativa L. The most pharmacologically active constituents are TETRAHYDROCANNABINOL; CANNABINOL; and CANNABIDIOL.Diuron: A pre-emergent herbicide.Herbicides: Pesticides used to destroy unwanted vegetation, especially various types of weeds, grasses (POACEAE), and woody plants. Some plants develop HERBICIDE RESISTANCE.Linuron: A selective pre- and post-emergence herbicide. (From Merck Index, 11th ed)Phenylurea Compounds: Compounds that include the amino-N-phenylamide structure.Comamonadaceae: A family of gram-negative aerobic bacteria in the class BETA PROTEOBACTERIA, encompassing the acidovorans rRNA complex. Some species are pathogenic for PLANTS.Methylurea Compounds: Urea compounds which are substituted with one or more methyl groups.Glutamine: A non-essential amino acid present abundantly throughout the body and is involved in many metabolic processes. It is synthesized from GLUTAMIC ACID and AMMONIA. It is the principal carrier of NITROGEN in the body and is an important energy source for many cells.Methionyl Aminopeptidases: Aminopeptidases that remove METHIONINE from the amino-terminus of a peptide chain, such as the initiator METHIONINE found on nascent peptide chains.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Proline: A non-essential amino acid that is synthesized from GLUTAMIC ACID. It is an essential component of COLLAGEN and is important for proper functioning of joints and tendons.Aminoacyltransferases: Enzymes that catalyze the transfer of an aminoacyl group from donor to acceptor resulting in the formation of an ester or amide linkage. EC 2.3.2.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Glutamate-Ammonia Ligase: An enzyme that catalyzes the conversion of ATP, L-glutamate, and NH3 to ADP, orthophosphate, and L-glutamine. It also acts more slowly on 4-methylene-L-glutamate. (From Enzyme Nomenclature, 1992) EC 6.3.1.2.GTP Cyclohydrolase: (GTP cyclohydrolase I) or GTP 7,8-8,9-dihydrolase (pyrophosphate-forming) (GTP cyclohydrolase II). An enzyme group that hydrolyzes the imidazole ring of GTP, releasing carbon-8 as formate. Two C-N bonds are hydrolyzed and the pentase unit is isomerized. This is the first step in the synthesis of folic acid from GTP. EC 3.5.4.16 (GTP cyclohydrolase I) and EC 3.5.4.25 (GTP cyclohydrolase II).Riboflavin: Nutritional factor found in milk, eggs, malted barley, liver, kidney, heart, and leafy vegetables. The richest natural source is yeast. It occurs in the free form only in the retina of the eye, in whey, and in urine; its principal forms in tissues and cells are as FLAVIN MONONUCLEOTIDE and FLAVIN-ADENINE DINUCLEOTIDE.Encyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)AmidinesBiopterin: A natural product that has been considered as a growth factor for some insects.Pterins: Compounds based on 2-amino-4-hydroxypteridine.AminohydrolasesPimelic Acids: A group of compounds that are derivatives of heptanedioic acid with the general formula R-C7H11O4.Diaminopimelic AcidMedlinePlus: NATIONAL LIBRARY OF MEDICINE service for health professionals and consumers. It links extensive information from the National Institutes of Health and other reviewed sources of information on specific diseases and conditions.Lysine: An essential amino acid. It is often added to animal feed.Carbon: A nonmetallic element with atomic symbol C, atomic number 6, and atomic weight [12.0096; 12.0116]. It may occur as several different allotropes including DIAMOND; CHARCOAL; and GRAPHITE; and as SOOT from incompletely burned fuel.Sarcina: A genus of gram-positive, anaerobic bacteria whose organisms divide in three perpendicular planes and occur in packets of eight or more cells. It has been isolated from soil, grains, and clinical specimens.Penicillins: A group of antibiotics that contain 6-aminopenicillanic acid with a side chain attached to the 6-amino group. The penicillin nucleus is the chief structural requirement for biological activity. The side-chain structure determines many of the antibacterial and pharmacological characteristics. (Goodman and Gilman's The Pharmacological Basis of Therapeutics, 8th ed, p1065)Pseudomonas aeruginosa: A species of gram-negative, aerobic, rod-shaped bacteria commonly isolated from clinical specimens (wound, burn, and urinary tract infections). It is also found widely distributed in soil and water. P. aeruginosa is a major agent of nosocomial infection.Penicillin G: A penicillin derivative commonly used in the form of its sodium or potassium salts in the treatment of a variety of infections. It is effective against most gram-positive bacteria and against gram-negative cocci. It has also been used as an experimental convulsant because of its actions on GAMMA-AMINOBUTYRIC ACID mediated synaptic transmission.Bacillus megaterium: A species of bacteria whose spores vary from round to elongate. It is a common soil saprophyte.HydrocarbonsDictionaries, ChemicalAgrochemicals: Chemicals used in agriculture. These include pesticides, fumigants, fertilizers, plant hormones, steroids, antibiotics, mycotoxins, etc.Dictionaries, MedicalDictionaries as Topic: Lists of words, usually in alphabetical order, giving information about form, pronunciation, etymology, grammar, and meaning.Biopharmaceutics: The study of the physical and chemical properties of a drug and its dosage form as related to the onset, duration, and intensity of its action.Pharmaceutical Preparations: Drugs intended for human or veterinary use, presented in their finished dosage form. Included here are materials used in the preparation and/or formulation of the finished dosage form.Biological Ontologies: Structured vocabularies describing concepts from the fields of biology and relationships between concepts.

Reduced pyrazinamidase activity and the natural resistance of Mycobacterium kansasii to the antituberculosis drug pyrazinamide. (1/2620)

Pyrazinamide (PZA), an analog of nicotinamide, is a prodrug that requires conversion to the bactericidal compound pyrazinoic acid (POA) by the bacterial pyrazinamidase (PZase) activity of nicotinamidase to show activity against Mycobacterium tuberculosis. Mutations leading to a loss of PZase activity cause PZA resistance in M. tuberculosis. M. kansasii is naturally resistant to PZA and has reduced PZase activity along with an apparently detectable nicotinamidase activity. The role of the reduction in PZase activity in the natural PZA resistance of M. kansasii is unknown. The MICs of PZA and POA for M. kansasii were determined to be 500 and 125 micrograms/ml, respectively. Using [14C]PZA and [14C]nicotinamide, we found that M. kansasii had about 5-fold-less PZase activity and about 25-fold-less nicotinamidase activity than M. tuberculosis. The M. kansasii pncA gene was cloned on a 1.8-kb BamHI DNA fragment, using M. avium pncA probe. Sequence analysis showed that the M. kansasii pncA gene encoded a protein with homology to its counterparts from M. tuberculosis (69.9%), M. avium (65.6%), and Escherichia coli (28.5%). Transformation of naturally PZA-resistant M. bovis BCG with M. kansasii pncA conferred partial PZA susceptibility. Transformation of M. kansasii with M. avium pncA caused functional expression of PZase and high-level susceptibility to PZA, indicating that the natural PZA resistance in M. kansasii results from a reduced PZase activity. Like M. tuberculosis, M. kansasii accumulated POA in the cells at an acidic pH; however, due to its highly active POA efflux pump, the naturally PZA-resistant species M. smegmatis did not. These findings suggest the existence of a weak POA efflux mechanism in M. kansasii.  (+info)

Thermostability reinforcement through a combination of thermostability-related mutations of N-carbamyl-D-amino acid amidohydrolase. (2/2620)

For the improvement of N-carbamyl-D-amino acid amidohydrolase (DCase), which can be used for the industrial production of D-amino acids, the stability of DCase from Agrobacterium sp. KNK712 was improved through various combinations of thermostability-related mutations. The thermostable temperature (defined as the temperature on heat treatment for 10 min that caused a decrease in the DCase activity of 50%) of the enzyme which had three amino acids, H57Y, P203E, and V236A, replaced was increased by about 19 degrees C. The mutant DCase, designated as 455M, was purified and its enzymatic properties were studied. The enzyme had highly increased stability against not only temperature but also pH, the optimal temperature of the enzyme being about 75 degrees C. The substrate specificity of the enzyme for various N-carbamyl-D-amino acids was changed little in comparison with that of the native enzyme. Enzymochemical parameters were also measured.  (+info)

Insertion analysis of putative functional elements in the promoter region of the Aspergillus oryzae Taka-amylase A gene (amyB) using a heterologous Aspergillus nidulans amdS-lacZ fusion gene system. (3/2620)

Expression of the Taka-amylase A gene (amyB) of Aspergillus oryzae is induced by starch or maltose. The A. oryzae amyB gene promoter contains three highly conserved sequences, designated Regions I, II, and III, compared with promoter regions of the A. oryzae glaA encoding glucoamylase and the agdA encoding alpha-glucosidase. To identify the function of these sequences within the amyB promoter, various fragments containing conserved sequences in the amyB promoter were introduced into the upstream region of the heterologous A. nidulans amdS gene (encoding acetamidase) fused to the Escherichia coli lacZ gene as a reporter. Introduction of the sequence between -290 to -233 (the number indicates the distance in base pairs from the translation initiation point (+1)) containing Region III significantly increased the expression of the lacZ reporter gene in the presence of maltose. The sequence between -377 to -290 containing Region I also increased the lacZ activity, but its maltose inducibility was less than that of Region III. The sequence between -233 to -181 containing Region II had no effect on the expression. These results indicated that Region III is most likely involved in the maltose induction of the amyB gene expression.  (+info)

Human biotinidase isn't just for recycling biotin. (4/2620)

For years, the major role of biotin has been as the coenzyme for four carboxylases in humans. Although there has been evidence that biotin might have other functions, none has been firmly established. The discovery that human serum biotinidase has biotinyl-transferase activity, in addition to biotinidase hydrolase activity, presents new possibilities for the role of biotinidase in biotin metabolism. Specific transfer of biotin to histones by biotinidase provides a possible explanation for why biotin is found in the nucleus and the nature of its role in the regulation of protein transcription. Future studies will help to determine the functions of biotinidase in biotin metabolism and in disease states.  (+info)

IAR3 encodes an auxin conjugate hydrolase from Arabidopsis. (5/2620)

Amide-linked conjugates of indole-3-acetic acid (IAA) are putative storage or inactivation forms of the growth hormone auxin. Here, we describe the Arabidopsis iar3 mutant that displays reduced sensitivity to IAA-Ala. IAR3 is a member of a family of Arabidopsis genes related to the previously isolated ILR1 gene, which encodes an IAA-amino acid hydrolase selective for IAA-Leu and IAA-Phe. IAR3 and the very similar ILL5 gene are closely linked on chromosome 1 and comprise a subfamily of the six Arabidopsis IAA-conjugate hydrolases. The purified IAR3 enzyme hydrolyzes IAA-Ala in vitro. iar 3 ilr1 double mutants are more resistant than either single mutant to IAA-amino acid conjugates, and plants overexpressing IAR3 or ILR1 are more sensitive than is the wild type to certain IAA-amino acid conjugates, reflecting the overlapping substrate specificities of the corresponding enzymes. The IAR3 gene is expressed most strongly in roots, stems, and flowers, suggesting roles for IAA-conjugate hydrolysis in those tissues.  (+info)

Characterization of a novel rat brain glycosylphosphatidylinositol-anchored protein (Kilon), a member of the IgLON cell adhesion molecule family. (6/2620)

In the central nervous system, many cell adhesion molecules are known to participate in the establishment and remodeling of the neural circuit. Some of the cell adhesion molecules are known to be anchored to the membrane by the glycosylphosphatidylinositol (GPI) inserted to their C termini, and many GPI-anchored proteins are known to be localized in a Triton-insoluble membrane fraction of low density or so-called "raft." In this study, we surveyed the GPI-anchored proteins in the Triton-insoluble low density fraction from 2-week-old rat brain by solubilization with phosphatidylinositol-specific phospholipase C. By Western blotting and partial peptide sequencing after the deglycosylation with peptide N-glycosidase F, the presence of Thy-1, F3/contactin, and T-cadherin was shown. In addition, one of the major proteins, having an apparent molecular mass of 36 kDa after the peptide N-glycosidase F digestion, was found to be a novel protein. The result of cDNA cloning showed that the protein is an immunoglobulin superfamily member with three C2 domains and has six putative glycosylation sites. Since this protein shows high sequence similarity to IgLON family members including LAMP, OBCAM, neurotrimin, CEPU-1, AvGP50, and GP55, we termed this protein Kilon (a kindred of IgLON). Kilon-specific monoclonal antibodies were produced, and Western blotting analysis showed that expression of Kilon is restricted to brain, and Kilon has an apparent molecular mass of 46 kDa in SDS-polyacrylamide gel electrophoresis in its expressed form. In brain, the expression of Kilon is already detected in E16 stage, and its level gradually increases during development. Kilon immunostaining was observed in the cerebral cortex and hippocampus, in which the strongly stained puncta were observed on dendrites and soma of pyramidal neurons.  (+info)

Evidence for the existence of an unfolding intermediate state for aminoacylase during denaturation in guanidine solutions. (7/2620)

The equilibrium unfolding of pig kidney aminoacylase in guanidinium chloride (GdmCl) solutions was studied by following the fluorescence and circular dichroism (CD). At low concentrations of GdmCl, less than 1.0 M, the fluorescence intensity decreased with a slight red shift of the emission maximum (from 335 to 340 nm). An unfolding intermediate was observed in low concentrations of denaturant (between 1.2 and 1.6 M GdmCl). This intermediate was characterized by a decreased fluorescence emission intensity, a red-shifted emission maximum, and increased binding of the fluorescence probe 1-anilino-8-naphthalenesulfonate. No significant changes of the secondary structure were indicated by CD measurement. This conformation state is similar to a molten globule state which may exist in the pathway of protein folding. Further changes in the fluorescence properties occurred at higher concentrations of GdmCl, more than 1.6 M, with a decrease in emission intensity and a significant red shift of the emission maximum from 340 to 354 nm. In this stage, the secondary structure was completely broken. A study of apo-enzyme (Zn2+-free enzyme) produced similar results. However, comparison of the changes of the fluorescence emission spectra of native (Holo-) enzyme with Zn2+-free (Apo-) enzyme at low GdmCl concentrations showed that the structure of the Holo-enzyme was more stable than that of the Apo-enzyme.  (+info)

Processing of the fibrillin-1 carboxyl-terminal domain. (8/2620)

To investigate the processing and general properties of the fibrillin-1 carboxyl-terminal domain, three protein expression constructs have been developed as follows: one without the domain, one with the domain, and one with a mutation near the putative proteolytic processing site. The constructs have been expressed in two eukaryotic model systems, baculoviral and CHO-K1. Post-translational modifications that normally occur in fibrillin-1, including glycosylation, signal peptide cleavage, and carboxyl-terminal processing, occur in the three constructs in both cell systems. Amino-terminal sequencing of secreted protein revealed leader sequence processing at two sites, a primary site between Gly-24/Ala-25 and a secondary site of Ala-27/Asn-28. Processing of the carboxyl-terminal domain could be observed by migration differences in SDS-polyacrylamide gel electrophoresis and was evident in both mammalian and insect cells. Immunological identification by Western blotting confirmed the loss of the expected region. The failure of both cell systems to process the mutant construct shows that the multi-basic sequence is the site of proteolytic processing. Cleavage of the fibrillin-1 carboxyl-terminal domain occurred intracellularly in CHO-K1 cells in an early secretory pathway compartment as demonstrated by studies with secretion blocking agents. This finding, taken with the multi-basic nature of the cleavage site and observed calcium sensitivity of cleavage, suggests that the processing enzyme is a secretory pathway resident furin-like protease.  (+info)

cansSAR 3D Structure of 6DY2_B | GUINEA PIG N-ACYLETHANOLAMINE-HYDROLYZING ACID AMIDASE (NAAA) COVALENTLY BOUND TO BETA-LACTAM INHIBITOR ARN726 | 6DY2
Goat Polyclonal Anti-ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody. Validated: WB, IP. Tested Reactivity: Mouse. 100% Guaranteed.
Hu X, Xu X, Zhu G, Atzler D, Kimoto M, Chen J, Schwedhelm E, Lüneburg N, Böger RH, Zhang P, et al. Vascular endothelial-specific dimethylarginine dimethylaminohydrolase-1- deficient mice reveal that vascular endothelium plays an important role in removing asymmetric dimethylarginine. Circulation [Internet]. 2009;(22):2222-2229.
MALDI-TOF MS analysis of the human NAAA zymogen (47.7 kDa) treated with peptide-N-glycosidase F (PNGase F) identified 4 glycosylation sites, and acid cleavage of the zymogen into alpha- and beta-subunits (14.6 and 33.3 kDa) activated the enzyme ...
Sphingolipid ceramide N-deacylase (SCDase) is derived from Pseudomonas and hydrolyzes the N-acyl linkage between fatty acids and sphingosine bases in ceramides of various sphingolipids. The enzyme also catalyzes the reverse reaction and possesses transacylation activity. SCDase acts on various acidic and neutral glycosphingolipids and sphingomyelin; however, it exhibits low activity with ceramides.. ...
Sphingolipid ceramide N-deacylase (SCDase) is derived from Pseudomonas and hydrolyzes the N-acyl linkage between fatty acids and sphingosine bases in ceramides of various sphingolipids. The enzyme also catalyzes the reverse reaction and possesses transacylation activity. SCDase acts on various acidic and neutral glycosphingolipids and sphingomyelin; however, it exhibits low activity with ceramides.. ...
TY - JOUR. T1 - Dimethylarginine dimethylaminohydrolase and endothelial dysfunction in failing hearts. AU - Chen, Yingjie. AU - Li, Yunfang. AU - Zhang, Ping. AU - Traverse, Jay H.. AU - Hou, Mingxiao. AU - Xu, Xin. AU - Kimoto, Masumi. AU - Bache, Robert J. PY - 2005/11/1. Y1 - 2005/11/1. N2 - Congestive heart failure (CHF) is associated with impaired endothelium-dependent nitric oxide (NO)-mediated vasodilation (endothelial dysfunction). We hypothesized that coronary endothelial dysfunction in CHF may be due in part to decreased dimethylarginine dimethylaminohydrolase (DDAH), the enzyme that degrades endogenous inhibitors of NO synthase (NOS), including asymmetric dimethylarginine. Coronary blood flow and the endothelium-dependent vasodilator response to acetylcholine were studied in dogs in which CHF was produced by rapid ventricular pacing for 4 wk. Coronary flow and myocardial O2 consumption at rest and during treadmill exercise were decreased after development of CHF, and the vasodilator ...
Fatty acid amide hydrolase (FAAH) is responsible for hydrolysis of endocannabinoid, anandamide (AEA), and N-acyl ethanolamines such as palmitoylethanolamine (PEA) and N-oleoylethanolamide (OEA). Genetic deletion or pharmacological inactivation of FAAH shows site-specific elevation of AEA that plays a role in the modulation of pain and other neurodegenerative disorders. The review elaborates recent progress and current status of diverse structural classes of reversible and irreversible FAAH inhibitors. The discussion also addresses ligand-enzyme active site interactions and mechanism of enzyme inactivation, emerging approaches to novel FAAH inhibitors, and ongoing efforts to address gaps in therapeutic utility of FAAH inhibitors.
Several factors contribute to the deterioration in synaptic plasticity which accompanies age and one of these is neuroinflammation. This is characterized by increased microglial activation associated with increased production of proinflammatory cytokines like interleukin-1β (IL-1β). In aged rats these neuroinflammatory changes are associated with a decreased ability of animals to sustain long-term potentiation (LTP) in the dentate gyrus. Importantly, treatment of aged rats with agents which possess anti-inflammatory properties to decrease microglial activation, improves LTP. It is known that endocannabinoids, such as anandamide (AEA), have anti-inflammatory properties and therefore have the potential to decrease the age-related microglial activation. However, endocannabinoids are extremely labile and are hydrolyzed quickly after production. Here we investigated the possibility that inhibiting the degradation of endocannabinoids with the fatty acid amide hydrolase (FAAH) inhibitor, URB597, could
As a member of the transient receptor potential (TRP) ion channel superfamily, the ligand-gated ion channel TRPA1 has been implicated in nociceptive function and pain states. The endogenous ligands that activate TRPA1 remain unknown. However, various agonists have been identified, including environmental irritants (e.g., acrolein) and ingredients of pungent natural products [e.g., allyl isothiocyanate (ITC), cinnamaldehyde, allicin, and gingerol]. In general, these agents are either highly reactive, nonselective, or not potent or efficacious, significantly limiting their utilities in the study of TRPA1 channel properties and biological functions. In a search for novel TRPA1 agonists, we identified 3 -carbamoylbiphenyl-3-yl cyclohexylcarbamate (URB597), a potent and systemically active inhibitor of fatty acid amide hydrolase (FAAH). This enzyme is responsible for anandamide degradation and therefore has been pursued as an antinociceptive and antiepileptic drug target. Using Ca influx assays and patch
Background Recent data have indicated that there may be a dysregulation of endocannabinoid metabolism in cancer. Here we have investigated the expression of the endocannabinoid metabolising enzyme fatty acid amide hydrolase (FAAH) in a well characterised tissue microarray from patients diagnosed with prostate cancer at transurethral resection for voiding problems. Methodology/Principal Findings FAAH immunoreactivity (FAAH-IR) was assessed in formalin-fixed paraffin-embedded non-malignant and tumour cores from 412 patients with prostate cancer. CB1 receptor immunoreactivity (CB1IR) scores were available for this dataset. FAAH-IR was seen in epithelial cells and blood vessel walls but not in the stroma. Tumour epithelial FAAH-IR was positively correlated with the disease severity at diagnosis (Gleason score, tumour stage, % of the specimen that contained tumour) for cases with mid-range CB1IR scores, but not for those with high CB1IR scores. For the 281 cases who only received palliative therapy at the
Lanopepden, also known as GSK-1322322 or GSK-322, is a potent and selective peptide deformylase inhibitor with good in vitro activity against bacteria associated with community-acquired pneumonia and skin infections. GSK1322322 had bactericidal activity against S. pneumoniae, H. influenzae, S. pyogenes, and S. aureus, demonstrating a ≥ 3-log(10) decrease in the number of CFU/ml at 4× MIC within 24 h in 29 of the 33 strains tested. GSK1322322 represents a valuable alternative therapy for the treatment of infectious diseases caused by drug-resistant pathogens.
FAAH (fatty acid amide hydrolase) is an integral membrane enzyme that degrades the fatty acid amide family of signaling lipids, including the endocannabinoid anandamide. Genetic or pharmacological inactivation of FAAH leads to analgesic, anti-inflammatory, anxiolytic, and antidepressant phenotypes in rodents without showing the undesirable side effects observed with direct cannabinoid receptor agonists, indicating that FAAH may represent an attractive therapeutic target for treatment of pain, inflammation, and other central nervous system disorders.
This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. [provided by RefSeq, Jul 2008 ...
Fatty acid amide hydrolase (FAAH) knockout mice are prone to excess energy storage and adiposity, whereas mutations in FAAH are associated with obesity in humans. However, the molecular mechanism by which FAAH affects energy expenditure (EE) remains unknown. Here we show that reduced energy expenditure in FAAH(-/-) mice could be attributed to decreased circulating triiodothyronine and thyroxine concentrations secondary to reduced mRNA expression of both pituitary thyroid-stimulating hormone and hypothalamic thyrotropin-releasing hormone. These reductions in the hypothalamic-pituitary-thyroid axis were associated with activation of hypothalamic peroxisome proliferating-activated receptor γ (PPARγ), and increased hypothalamic deiodinase 2 expression. Infusion of NAEs (anandamide and palmitoylethanolamide) recapitulated increases in PPARγ-mediated decreases in EE. FAAH(-/-) mice were also prone to diet-induced hepatic insulin resistance, which could be attributed to increased hepatic ...
Hu X, Atzler D, Xu X, Zhang P, Guo H, Lu Z, Fassett J, Schwedhelm E, Böger RH, Bache RJ, et al. Dimethylarginine dimethylaminohydrolase-1 is the critical enzyme for degrading the cardiovascular risk factor asymmetrical dimethylarginine. Arteriosclerosis, Thrombosis, and Vascular Biology [Internet]. 2011;(7):1540-1546. 访问链接 SCI被引用次数:52 ...
Protein synthesis proceeds after formylation of methionine by methionyl-tRNA formyl transferase (FMT) and transfer of the charged initiator f-met tRNA to the ribosome. In eubacteria and eukaryotic organelles the product of this gene, peptide deformylase (PDF), removes the formyl group from the initiating methionine of nascent peptides. In eubacteria, deformylation of nascent peptides is required for subsequent cleavage of initiating methionines by methionine aminopeptidase. The discovery that a natural inhibitor of PDF, actinonin, acts as an antimicrobial agent in some bacteria has spurred intensive research into the design of bacterial-specific PDF inhibitors. In human cells, only mitochondrial proteins have N-formylation of initiating methionines. Protein inhibitors of PDF or siRNAs of PDF block the growth of cancer cell lines but have no effect on normal cell growth. In humans, PDF function may therefore be restricted to rapidly growing cells. [provided by RefSeq, Nov 2008 ...
Amides/chemistry/pharmacology, Amidohydrolases/antagonists & inhibitors/chemistry/metabolism, Amines/chemistry/pharmacology, Animals, Arachidonic Acids/metabolism, Binding Sites, Drug Design, Endocannabinoids/*chemistry/*metabolism/pharmacology, Esters/chemistry/pharmacology, Ethers/chemistry/pharmacology, Glycerides/metabolism, Humans, Ligands, Monoacylglycerol Lipases/metabolism, Polyunsaturated Alkamides, Receptors; Cannabinoid/chemistry/drug effects ...
Title:Recent Process in the Inhibitors of UDP-3-O-(R-3-hydroxyacyl)-Nacetylglucosamine Deacetylase (LpxC) Against Gram-Negative Bacteria. VOLUME: 18 ISSUE: 4. Author(s):Fang Liu and Shutao Ma*. Affiliation:Department of Medicinal Chemistry, Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Shandong University, 44 West Culture Road, Jinan 250012, Department of Medicinal Chemistry, Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Shandong University, 44 West Culture Road, Jinan 250012. Keywords:Anti-bacterial agent, biosynthesis, UDP-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine deacetylase (LpxC), LpxC inhibitors, Gram-negative bacterial, lipid A biosynthesis.. Abstract:Infections caused by pathogenic bacteria are a major health concern throughout the world. There is a great need to develop novel antibacterial agents with new mechanisms of action. Lipopolysaccharides (LPS) are the main component of the ...
Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
UCL Discovery is UCLs open access repository, showcasing and providing access to UCL research outputs from all UCL disciplines.
Ureases, functionally, belong to the superfamily of amidohydrolases and phosphotriesterases. It is an enzyme that catalyzes the hydrolysis of urea into carbon dioxide and
Creatininase catalyzes the conversion of creatinine (a biosensor for kidney function) to creatine via a two-step mechanism: water addition followed by ring opening. Water addition is common to other known cyclic amidohydrolases, but the precise mechanism for ring opening is still under debate. The proton donor in this step is either His178 or a water molecule bound to one of the metal ions, and th ...
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MicroRNA (miRNA) disorder is associated with a variety of human being illnesses, including malignancy. miR-671-5p lead in a change from epithelial-to-mesenchymal changeover (EMT) to mesenchymal-to-epithelial changeover (MET) phenotypes in MDA-MB-231 breasts malignancy cells and caused S-phase police arrest. Furthermore, miR-671-5p sensitive breasts malignancy cells to cisplatin, 5-fluorouracil (5-FU) and epirubicin publicity. Host cell reactivation (HCR) assays demonstrated that miR-671-5p decreases DNA restoration ability in post-drug revealed breasts malignancy cells. cDNA microarray data exposed that differentially indicated genetics when miR-671-5p was transfected are linked with cell growth, breach, cell routine, and EMT. These data suggest that miR-671-5p features as a growth suppressor miRNA in breasts cancer tumor by straight concentrating on FOXM1. Therefore, miR-671-5p might serve as a new therapeutic focus on for breasts cancer tumor administration. (DCIS), and culminates in the ...
MicroRNA (miRNA) disorder is associated with a variety of human being illnesses, including malignancy. miR-671-5p lead in a change from epithelial-to-mesenchymal changeover (EMT) to mesenchymal-to-epithelial changeover (MET) phenotypes in MDA-MB-231 breasts malignancy cells and caused S-phase police arrest. Furthermore, miR-671-5p sensitive breasts malignancy cells to cisplatin, 5-fluorouracil (5-FU) and epirubicin publicity. Host cell reactivation (HCR) assays demonstrated that miR-671-5p decreases DNA restoration ability in post-drug revealed breasts malignancy cells. cDNA microarray data exposed that differentially indicated genetics when miR-671-5p was transfected are linked with cell growth, breach, cell routine, and EMT. These data suggest that miR-671-5p features as a growth suppressor miRNA in breasts cancer tumor by straight concentrating on FOXM1. Therefore, miR-671-5p might serve as a new therapeutic focus on for breasts cancer tumor administration. (DCIS), and culminates in the ...
Fatty acid amide hydrolase (FAAH) terminates the endocannabinoid signaling pathway that regulates numerous neurobehavioral processes in animals by hydrolyzing a class of lipid mediators, N-acylethanolamines (NAEs). Recent identification of an Arabidopsis FAAH homologue (AtFAAH) and several studies, especially those using AtFAAH overexpressing and knock-out lines suggest that a FAAH-mediated pathway exists in plants for the metabolism of endogenous NAEs. Here, I provide evidence to support this concept by identifying candidate FAAH cDNA sequences in diverse plant species. NAE amidohydrolase assays confirmed that several of the proteins encoded by these cDNAs indeed catalyzed the hydrolysis of NAEs in vitro. Kinetic parameters, inhibition properties, and substrate specificities of the plant FAAH enzymes were very similar to those of mammalian FAAH. Five amino acid residues determined to be important for catalysis by rat FAAH were absolutely conserved within the plant FAAH sequences. Site-directed mutation
In the DDAH transgenic animals, we observed a 10% increase in basal heart rate. The increase in heart rate may reflect a heightened sympathetic tone, activated in response to the reduction in vascular resistance. The increase in heart rate is balanced by a 10% reduction in stroke volume, so that cardiac output does not change. The reduction in stroke volume may be attributable to the shortened time for diastolic filling of the heart. Increased compliance of the venous bed, secondary to increased venous NOS activity, may also contribute to the reduction in diastolic filling.. Alternatively, the increase in heart rate may be compensating for a reduction in ventricular contractility. NO at submillimolar levels is known to reduce myocardial contractility.36-38 In patients with heart failure, activation of inducible NOS can have significant effects on left ventricular systolic and diastolic function.39,40 However, the effect of the constitutive forms of NOS on ventricular function are much more ...
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Amidases catalyse the hydrolysis of amides to the corresponding carboxylic acid and ammonia. They exist in all kingdoms of the living world but have been most extensively characterised amongst the bacteria.. A number of studies of amidase classification (10.1016/S0167-4838(96)00145-8)(10.1046/j.1365-2672.2001.01378.x)(10.1186/gb-2001-2-1-reviews0001) have revealed that the bacterial aliphatic amidases (broadly classed as acylamide amidohydrolase, EC 3.5.1.4) are made up of two types.. The first group, the nitrilase-related family, includes the aliphatic amidases, hydrolysing only short-chain aliphatic amides (10.1046/j.1365-2672.2001.01378.x). The enzymes are typically homohexamers of approximately 230kDa, and contain a Cys166 residue (Pseudomonas aeruginosa amidase numbering), conserved across both nitrilase and amidase. This residue is believed to act as the catalytic nucleophile. The amidases from P. aeruginosa (10.1016/0014-5793(87)80163-1)(10.1016/j.ijbiomac.2003.08.002[c/ite], Rhodococcus ...
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Angiotensin-converting enzyme inhibitors (ACEIs) are the main reason behind angioedema (AE) induced by medications. painful and asymmetrical. There is certainly neither pruritus nor urticaria. A localized love from the intestines can 1159824-67-5 manufacture be done but it generally affects the facial skin the tongue and all of those other 1159824-67-5 manufacture ear nasal area and …Read More. ...
Fatty acid amide hydrolase (FAAH) is an integral membrane serine hydrolase that degrades the fatty acid amide family of signaling lipids, including the endocannabinoid anandamide. Genetic or pharmacological inactivation of FAAH leads to analgesic and anti-inflammatory phenotypes in rodents without s …
This invention relates to the crystal structure of a plant peptide deformylase polypeptide and methods of using the structure to design compounds that modulate the activity of the polypeptide.
Definition of N-succinylglutamate desuccinylase with photos and pictures, translations, sample usage, and additional links for more information.
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RefSeq Summary (NM_013974): This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. [provided by RefSeq, Jul 2008]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF070667.1, AF087894.1 [ECO:0000332] RNAseq introns :: single sample supports all introns ERS025083 [ECO:0000348] ##Evidence-Data-END# ...
Complete information for FAAH2 gene (Protein Coding), Fatty Acid Amide Hydrolase 2, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
1H70: Structural Insights Into the Hydrolysis of Cellular Nitric Oxide Synthase Inhibitors by Dimethylarginine Dimethylaminohydrolase
1IX1: Crystal structure of peptide deformylase from Staphylococcus aureus in complex with actinonin, a naturally occurring antibacterial agent
Sigma-Aldrich offers abstracts and full-text articles by [Yuanheng Cai, Peter Trodler, Shimin Jiang, Weiwen Zhang, Yan Wu, Yinhua Lu, Sheng Yang, Weihong Jiang].
Sep 04, 2019 (The Expresswire) -- Global Fatty Acid Amide (FAA) Market Report, History and Forecast 2014-2025, Breakdown Data by Manufacturers, Key Regions,...
Heme oxygenase-1 (HO-1) is a tension response antioxidant enzyme which catalyzes the degradation of heme released during irritation. activity or considerably restrict bacterial development in liquid lifestyle. Together, the above mentioned results reveal mammalian HO-1 being a potential focus on for host-directed monotherapy and adjunctive therapy of tuberculosis and recognize the immune system 911417-87-3 response as a crucial regulator of the function. IMPORTANCE There is absolutely no dependable vaccine against tuberculosis (TB), and regular antibiotic therapy can be implemented at least 6?a few months. This extended treatment period can result in noncompliance leading to relapsed disease aswell as the introduction of multidrug level of resistance. Thus, there can be an urgent dependence on improved healing regimens that may quicker and effectively control in contaminated patients. Right here, we explain a potential technique for dealing with TB predicated on pharmacological inhibition from ...
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FAAH antibody (fatty acid amide hydrolase) for IHC-P, WB. Anti-FAAH pAb (GTX55611) is tested in Human, Mouse, Rat samples. 100% Ab-Assurance.
Human mitochondrial peptide deformylase (PDF) has been proposed as a novel cancer therapeutic target. However, very little is known about its expression and regulation in human tissues. The purpose of this study was to characterize the expression pattern of PDF in cancerous tissues and to identify mechanisms that regulate its expression. The mRNA expression levels of PDF and methionine aminopeptidase 1D (MAP1D), an enzyme involved in a related pathway with PDF, were determined using tissue panels containing cDNA from patients with various types of cancer (breast, colon, kidney, liver, lung, ovarian, prostate, or thyroid) and human cell lines. Protein levels of PDF were also determined in 2 colon cancer patients via western blotting. Colon cancer cells were treated with inhibitors of ERK, Akt, and mTOR signaling pathways and the resulting effects on PDF and MAP1D mRNA levels were determined by qPCR for colon and lung cancer cell lines. Finally, the effects of a PDF inhibitor, actinonin, on the
Although the study of Hu et al26 complements previous studies using the endothelial-specific DDAH1 knockout and heterozygous DDAH1-deficient mice,28,29 it also raises some interesting questions. First, there is a discrepancy between this study and a previous one that suggested that the global DDAH1 knockout was lethal.29 It is possible that in the previous study (in which exon 1 of DDAH was targeted), the deletion might have adversely affected another genomic region necessary for embryogenesis.. If DDAH2 does not compensate for the loss of DDAH1, what may be its function? The literature is mixed regarding the importance of DDAH2 in the metabolism of ADMA.6,30-32 Overexpression of DDAH2 improves endothelium-dependent vasorelaxation and increases NO synthesis, whereas small interfering RNA knockdown of DDAH2 reduces NO synthesis.31-33 However, the story becomes more interesting with recent evidence that both DDAH1 and DDAH2 manifest protein-protein interactions that may affect the NOS pathway ...
A new paper out of a group in Ireland, led by Drs. Susan Joyce and Cormac Gahan, has found a link between the bacterial enzyme bile salt hydrolase (BSH) and host lipid metabolism. Prior to this study, the authors had outlined the role of BSH, an enzyme limited to species of intestinal microbiota, in gut flora survival - this enzyme is an essential reaction in the metabolism of bile acids, allowing the microbes to tolerate bile, which is crucial to the digestion of fats. BSH activity is often associated with the bacteria used as probiotics for humans and animals, and is considered to be the contributing factor for their survival in the intestines. The authors took their characterization of BSH one step further by using mice to test the effect of BSH on microbe-mediated host lipid metabolism ...
Thus, the two substrates of this enzyme are GTP and H2O, whereas its 3 products are formate, 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine, and diphosphate.. This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in cyclic amidines. The systematic name of this enzyme class is GTP 7,8-8,9-dihydrolase (diphosphate-forming). Other names in common use include guanosine triphosphate cyclohydrolase II, and GTP-8-formylhydrolase. This enzyme participates in riboflavin metabolism.. ...
The systematic name of this enzyme class is N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase. This enzyme is also called N ...
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3.[7] Furthermore, HDAC4 is required for TGFbeta1-induced myofibroblastic differentiation.[8] ...
The systematic name of this enzyme class is N-(long-chain-acyl)ethanolamine amidohydrolase. Other names in common use include N ... Schmid PC, Zuzarte-Augustin ML, Schmid HH (1985). "Properties of rat liver N-acylethanolamine amidohydrolase". J. Biol. Chem. ...
The domain is named after the acronym cysteine, histidine-dependent amidohydrolases/peptidases. Many of these proteins are ... L-glutamate-specific amidohydrolases". Trends Biochem. Sci. 28 (5): 230-4. doi:10.1016/s0968-0004(03)00062-8. PMID 12765833. ...
Ogawa J, Shimizu S, Yamada H (1993). "N-carbamoyl-D-amino acid amidohydrolase from Comamonas sp. E222c purification and ...
Yamamoto K, Oka M, Kikuchi T, Emi S (1995). "Cloning of the creatinine amidohydrolase gene from Pseudomonas sp. PS-7". Biosci. ... Creatininase is a member of the urease-related amidohydrolases, the family of hydrolases, those acting on carbon-nitrogen bonds ... The systematic name of this enzyme class is creatinine amidohydrolase. This enzyme is also called creatinine hydrolase. This ... PDB: 3NO4​; Joint Center for Structural Genomics (2010). "Crystal structure of a creatinine amidohydrolase (Npun_F1913) from ...
L-asparagine amidohydrolase) is an enzyme with systematic name N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine amidohydrolase. ... Mahadevan, S.; Tappel, A.L. (1967). "β-Aspartylglucosylamine amido hydrolase of rat liver and kidney". J. Biol. Chem. 242 (20 ... Tarentino, A.L.; Maley, F. (1969). "The purification and properties of a β-aspartyl N-acetylglucosylamine amidohydrolase from ... "Purification and properties of 4-L-aspartylglycosylamine amidohydrolase from hog kidney". Biochim. Biophys. Acta. 258 (2): 600- ...
This enzyme is also called glutathionylspermidine amidohydrolase (spermidine-forming). This enzyme participates in glutathione ...
The systematic name of this enzyme class is N-formyl-L-glutamate amidohydrolase. Other names in common use include beta-citryl- ... Hu L, Mulfinger LM, Phillips AT (1987). "Purification and properties of formylglutamate amidohydrolase from Pseudomonas putida ... beta-citryl-L-glutamate amidohydrolase, beta-citryl-L-glutamate amidase, beta-citrylglutamate amidase, and beta-citryl-L- ...
The systematic name of this enzyme class is benzoylagmatine amidohydrolase. Other names in common use include acylagmatine ... Hydrolysis of bleomycin B2 by a Fusarium acylagmatine amidohydrolase". J. Antibiot. 26 (2): 117-119. doi:10.7164/antibiotics. ... amidohydrolase, and acylagmatine deacylase. Takita T; Takahashi, Y; Fujii, A; Saino, T; Shirai, T (1974). "Preparation of ...
"Entrez Gene: ASAH1 N-acylsphingosine amidohydrolase (acid ceramidase) 1". Zhou, J.; Tawk, M.; Tiziano, F. D.; Veillet, J.; ...
... (EC 3.5.2.1) is a zinc containing amidohydrolase. Its systemic name is barbiturate amidohydrolase (3-oxo-3- ... Although grouped into the naturally existing amidohydrolases, it demonstrates more homology with cyanuric acid amidohydrolase. ... Unlike other zinc containing amidohydrolases, the zinc binding motif of barbiturase is found on the carboxylic acid terminus, ... Soong, C.L.; Ogawa, J.; Sakuradani, E.; Shimizu, S. (2002). "Barbiturase, a novel zinc-containing amidohydrolase involved in ...
"Entrez Gene: ASAHL N-acylsphingosine amidohydrolase (acid ceramidase)-like". Human NAAA genome location and NAAA gene details ...
... s (EC 3.5.1.5), functionally, belong to the superfamily of amidohydrolases and phosphotriesterases. It is an enzyme that ... unification of a broad set of amidohydrolases related to urease". Proteins. 28 (1): 72-82. doi:10.1002/(SICI)1097-0134(199705) ...
The systematic name of this enzyme class is D-glutamine amidohydrolase. This enzyme participates in d-glutamine and d-glutamate ... Domnas A; Catimo EC (1965). "The behavior of amidohydrolases and L-glutamate in synchronized populations of Blastocladiella ...
"Entrez Gene: ASAH2 N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2". Human ASAH2 genome location and ASAH2 gene ...
The systematic name of this enzyme class is pentanamide amidohydrolase. This enzyme is also called valeramidase. Friedich CG; ...
The systematic name of this enzyme class is acylamide amidohydrolase. Other names in common use include acylamidase, acylase, ... In enzymology, an amidase (EC 3.5.1.4, acylamidase, acylase (misleading), amidohydrolase (ambiguous), deaminase (ambiguous), ... amidohydrolase, deaminase, fatty acylamidase, and N-acetylaminohydrolase. This enzyme participates in 6 metabolic pathways: ...
The systematic name of this enzyme class is nicotinamide-D-ribonucleotide amidohydrolase. Other names in common use include NMN ... Imai T (January 1973). "Purification and properties of nicotinamide mononucleotide amidohydrolase from Azotobacter vinelandii ... deamidase, nicotinamide mononucleotide deamidase, and nicotinamide mononucleotide amidohydrolase. This enzyme participates in ...
The systematic name of this enzyme class is chenodeoxycholoyltaurine amidohydrolase. This enzyme participates in bile acid ...
The systematic name of this enzyme class is N-carbamoylputrescine amidohydrolase. Other names in common use include ... Yanagisawa H; Suzuki Y (1982). "Preparation and properties of N-carbamylputrescine amidohydrolase from maize shoots". ...
The systematic name of this enzyme class is penicillin amidohydrolase. Other names in common use include penicillin acylase, ...
The systematic name of this enzyme class is mimosine amidohydrolase. Tangendjaja B, Lowry JB & Wills RH (1986). "Isolation of a ...
The systematic name of this enzyme class is formamide amidohydrolase. This enzyme participates in glyoxylate and dicarboxylate ...
Amidohydrolases (or amidases) are a type of hydrolase that acts upon amide bonds. They are categorized under EC number EC 3.5.1 ... Aminohydrolases Amidohydrolases at the US National Library of Medicine Medical Subject Headings (MeSH) CRISP Thesaurus 00000306 ... dead link] Amidohydrolase Overview from the EFI[permanent dead link] Molecular and Cellular Biology portal. ... and 3.5.2. Examples include: Beta-lactamase Histone deacetylase Urease The amidohydrolase superfamily is a large protein family ...
Maleamate amidohydrolase (EC 3.5.1.107, NicF) is an enzyme with systematic name maleamate amidohydrolase. This enzyme catalyses ... CS1 maint: Multiple names: authors list (link) Maleamate amidohydrolase at the US National Library of Medicine Medical Subject ...
... plural amidohydrolases) 1. Any of a class of hydrolases that act upon amide bonds. ... ... amidohydrolase. Noun (countable and uncountable, plural amidohydrolases). *Any of a class of hydrolases that act upon amide ... How would you define amidohydrolase? Add your definition here.. Please enable JavaScript to view the comments powered by Disqus ...
creatinine amidohydrolase (creatininase) protein [Rhizobium etli CFN 42]. * Record removed. The sequence YP_471106 is 100% ...
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
Amidohydrolase. A. 357. Staphylococcus aureus. Mutation(s): 0 Gene Names: SAOG_01574, SAV2580. EC: 3.5.1. ... Crystal structure of a putative amidohydrolase from Staphylococcus aureus. Qiu, W., Lam, R., Romanov, V., Lam, K., Soloveychik ... Crystal structure of a putative amidohydrolase from Staphylococcus aureus. *DOI: 10.2210/pdb3NUR/pdb ...
... is hydrolyzed by an amidohydrolase and its biological activity is lost. Previously, we partially purified the enzyme from ... Distribution of anandamide amidohydrolase in rat tissues with special reference to small intestine Biochim Biophys Acta. 1997 ... Anandamide (arachidonylethanolamide), an endogenous ligand for cannabinoid receptors, is hydrolyzed by an amidohydrolase and ...
CbiZ, an amidohydrolase enzyme required for salvaging the coenzyme B12 precursor cobinamide in archaea.. Woodson JD1, Escalante ... CbiZ, an amidohydrolase enzyme required for salvaging the coenzyme B12 precursor cobinamide in archaea ... CbiZ, an amidohydrolase enzyme required for salvaging the coenzyme B12 precursor cobinamide in archaea ... CbiZ, an amidohydrolase enzyme required for salvaging the coenzyme B12 precursor cobinamide in archaea ...
Protein N-terminal glutamine amidohydrolase (EC:3.5.1.122By similarity. Manual assertion inferred from sequence similarity toi ... "Crystal structure of human protein N-terminal glutamine amidohydrolase, an initial component of the N-end rule pathway.". Park ... sp,Q96HA8,NTAQ1_HUMAN Protein N-terminal glutamine amidohydrolase OS=Homo sapiens OX=9606 GN=WDYHV1 PE=1 SV=2 ...
Purification and properties of formylglutamate amidohydrolase from Pseudomonas putida.. L Hu, L M Mulfinger, A T Phillips ... Purification and properties of formylglutamate amidohydrolase from Pseudomonas putida. Message Subject (Your Name) has ...
Characterization of Four Bifunctional Plant IAM/PAM-Amidohydrolases Capable of Contributing to Auxin Biosynthesis by Beatriz ... "Characterization of Four Bifunctional Plant IAM/PAM-Amidohydrolases Capable of Contributing to Auxin Biosynthesis." Plants 3, ... Characterization of Four Bifunctional Plant IAM/PAM-Amidohydrolases Capable of Contributing to Auxin Biosynthesis. Plants 2014 ... Characterization of Four Bifunctional Plant IAM/PAM-Amidohydrolases Capable of Contributing to Auxin Biosynthesis. Plants. 2014 ...
... we use the amidohydrolase and enolase superfamilies as model systems; members of these superfamilies share a common TIM barrel ... Target Selection and Annotation for the Structural Genomics of the Amidohydrolase and Enolase Superfamilies J Struct Funct ... To study the substrate specificity of enzymes, we use the amidohydrolase and enolase superfamilies as model systems; members of ... To date, 20 unique amidohydrolase and 41 unique enolase structures have been determined, increasing the fraction of sequences ...
amidohydrolase domain containing 1. Synonyms: 1300019J08Rik. Gene nomenclature, locus information, and GO, OMIM, and PMID ...
In the final reaction, N-carbamoylputrescine is hydrolyzed to putrescine by N-carbamoylputrescine amidohydrolase (CPA, EC 3.5. ... In the final reaction, N-carbamoylputrescine is hydrolyzed to putrescine by N-carbamoylputrescine amidohydrolase (CPA, EC 3.5. ... 2000). Crystal structure of N-carbamyl-D-amino acid amidohydrolase with a novel catalytic framework common to amidohydrolases. ... N-carbamoylputrescine amidohydrolase enzymes belong to nitrilases, and more precisely, to C-N hydrolases breaking non-peptide ...
Characterization of the phenylurea hydrolases A and B: founding members of a novel amidohydrolase subgroup. Jeevan L. Khurana, ... Sequence analysis showed that these enzymes belong to the metal-dependent amidohydrolase superfamily, although they contain a ... Characterization of the phenylurea hydrolases A and B: founding members of a novel amidohydrolase subgroup ... Characterization of the phenylurea hydrolases A and B: founding members of a novel amidohydrolase subgroup ...
By fusing GPB to the CHAP (cysteine, histidine-dependent amidohydrolase/peptidase) catalytic domain of the PlyC lysin, we ... ClyJ Is a Novel Pneumococcal Chimeric Lysin with a Cysteine- and Histidine-Dependent Amidohydrolase/Peptidase Catalytic Domain ... ClyJ Is a Novel Pneumococcal Chimeric Lysin with a Cysteine- and Histidine-Dependent Amidohydrolase/Peptidase Catalytic Domain ... CHAP, cysteine, histidine-dependent amidohydrolases/peptidases; PlyCA CHAP, the CHAP domain from PlyCA (amino acids 314 to 465 ...
Recombinant Protein and Allantoate amidohydrolase Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody ... Allantoate amidohydrolase. Allantoate amidohydrolase ELISA Kit. Allantoate amidohydrolase Recombinant. Allantoate ... Below are the list of possible Allantoate amidohydrolase products. If you cannot find the target and/or product is not ... Also known as Allantoate amidohydrolase (Allantoate deiminase).. Escherichia coli is able to utilize allantoin as a sole ...
Crystal structure of human protein N-terminal glutamine amidohydrolase, an initial component of the N-end rule pathway.. ...
... Krämer, Andreas; Herzer, Jan ... all three putative acetylpolyamine amidohydrolases (APAHs) from P. aeruginosa have been expressed in enzymatic active form. The ...
Cyanuric acid amidohydrolase - Also known as CAH_MOOTA, Moth_2120. Responsible for the hydrolysis of cyanuric acid, an ...
Thus, the two substrates of this enzyme are GTP and H2O, whereas its 3 products are formate, 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine, and diphosphate.. This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in cyclic amidines. The systematic name of this enzyme class is GTP 7,8-8,9-dihydrolase (diphosphate-forming). Other names in common use include guanosine triphosphate cyclohydrolase II, and GTP-8-formylhydrolase. This enzyme participates in riboflavin metabolism.. ...
The systematic name of this enzyme class is N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase. This enzyme is also called N ...
Penicillin amidohydrolase productivity of four locally isolated bacterial species is described. Organisms were identified ... Penicillin amidohydrolase productivity of locally isolated bacterial species. Author. Z. A. Mahmood, D. Shaikh, S. M. S. Zoha. ... Penicillin amidohydrolase productivity of four locally isolated bacterial species is described. Organisms were identified ...
... all three putative acetylpolyamine amidohydrolases (APAHs) from P. aeruginosa have been expressed in enzymatic active form. The ... A new amidohydrolase from Bordetella or Alcaligenes strain FB188 with similarities to histone deacetylases. J Bacteriol. 2004; ... a Multiple Sequence Alignment of PA0321, PA1409 and PA3774 from P. aeruginosa, acetylpolyamine amidohydrolase APAH from M. ... 1a). Based on their sequence, PA0321 and PA1409 form a cluster with the verified functional acetylpolyamine amidohydrolase APAH ...
N-acyl-L-amino-acid amidohydrolase,Pig,Sus scrofa \ E2259p for more molecular products just contact us ... Product name : ELISA kit ACY1,ACY-1,Aminoacylase-1,N-acyl-L-amino-acid amidohydrolase,Pig,Sus scrofa ... E2259p ELISA kit ACY1,ACY-1,Aminoacylase-1,N-acyl-L-amino-acid amidohydrolase,Pig,Sus scrofa Ask technical file . ... We have also other products like : ELISA kit ACY1,ACY-1,Aminoacylase-1,N-acyl-L-amino-acid amidohydrolase,Pig,Sus scrofa. ...
  • On the basis of the similar catalytic function and existence of the rigidly conserved sequence, we propose a close evolutionary relationship among the functionally related amidohydrolases, including D-hydantoinase, dihydropyrimidinase, allantoinase and dihydroorotase. (kaist.ac.kr)
  • Sánchez-Parra B, Frerigmann H, Alonso M-MP, Loba VC, Jost R, Hentrich M, Pollmann S. Characterization of Four Bifunctional Plant IAM/PAM-Amidohydrolases Capable of Contributing to Auxin Biosynthesis. (mdpi.com)
  • The functionally related amidohydrolases, including D-hydantoinases, dihydropyrimidinases, allantoinases and dihydro-orotases, share a similar catalytic function of acting on the cyclic amide ring. (kaist.ac.kr)
  • As a particular sequence, one aspartic acid and four histidine residues are found to be rigidly conserved in the functionally related amidohydrolases. (kaist.ac.kr)
  • We aligned 16 amidohydrolases by taking account of the conservative substitution and found a number of highly conserved regions and invariant amino acid residues. (kaist.ac.kr)
  • Amidohydrolases (or amidases) are a type of hydrolase that acts upon amide bonds. (wikipedia.org)
  • Article on N-acylethanolamines metabolizing by lipoxygenase and amidohydrolase in competing pathways during cottonseed inbibition. (unt.edu)