A dye used to stain proteins in electrophoretic techniques. It is used interchangeably with its acid form.
Solutions for rinsing the mouth, possessing cleansing, germicidal, or palliative properties. (From Boucher's Clinical Dental Terminology, 4th ed)
Macrocyclic polyethers with the repeating unit of (-CH2-CH2-O)n where n is greater than 2 and some oxygens may be replaced by nitrogen, sulfur or phosphorus. These compounds are useful for coordinating CATIONS. The nomenclature uses a prefix to indicate the size of the ring and a suffix for the number of heteroatoms.
The salts or esters of salicylic acids, or salicylate esters of an organic acid. Some of these have analgesic, antipyretic, and anti-inflammatory activities by inhibiting prostaglandin synthesis.
The placing of a body or a part thereof into a liquid.
Organic salts and esters of benzenesulfonic acid.
Procedure of producing an imprint or negative likeness of the teeth and/or edentulous areas. Impressions are made in plastic material which becomes hardened or set while in contact with the tissue. They are later filled with plaster of Paris or artificial stone to produce a facsimile of the oral structures present. Impressions may be made of a full complement of teeth, of areas where some teeth have been removed, or in a mouth from which all teeth have been extracted. (Illustrated Dictionary of Dentistry, 1982)
Coverings for the hands, usually with separations for the fingers, made of various materials, for protection against infections, toxic substances, extremes of hot and cold, radiations, water immersion, etc. The gloves may be worn by patients, care givers, housewives, laboratory and industrial workers, police, etc.
Clothing designed to protect the individual against possible exposure to known hazards.
Personal devices for protection of the eyes from impact, flying objects, glare, liquids, or injurious radiation.
Fabric or other material used to cover the body.
Lenses designed to be worn on the front surface of the eyeball. (UMDNS, 1999)
A game in which two parties of players provided with curved or hooked sticks seek to drive a ball or puck through opposite goals. This applies to either ice hockey or field hockey.
An indicator and reagent. It has been used for several purposes including the determination of serum albumin concentrations
An indicator and reagent. It has been used in serum albumin determinations and as a pH indicator.
A cationic cytochemical stain specific for cell nuclei, especially DNA. It is used as a supravital stain and in fluorescence cytochemistry. It may cause mutations in microorganisms.
A dye that has been used as an industrial dye, a laboratory indicator, and a biological stain.
Compounds that contain the triphenylmethane aniline structure found in rosaniline. Many of them have a characteristic magenta color and are used as COLORING AGENTS.
Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.
Exclusive legal rights or privileges applied to inventions, plants, etc.
A photoprotein isolated from the bioluminescent jellyfish Aequorea. It emits visible light by an intramolecular reaction when a trace amount of calcium ion is added. The light-emitting moiety in the bioluminescence reaction is believed to be 2-amino-3-benzyl-5-(p-hydroxyphenyl)pyrazine (AF-350).
Techniques used for determining the values of photometric parameters of light resulting from LUMINESCENCE.
Elements with partially filled d orbitals. They constitute groups 3-12 of the periodic table of elements.
A novel composition, device, or process, independently conceived de novo or derived from a pre-existing model.
Property, such as patents, trademarks, and copyright, that results from creative effort. The Patent and Copyright Clause (Art. 1, Sec. 8, cl. 8) of the United States Constitution provides for promoting the progress of science and useful arts by securing for limited times to authors and inventors, the exclusive right to their respective writings and discoveries. (From Black's Law Dictionary, 5th ed, p1014)

The use of total protein stains as loading controls: an alternative to high-abundance single-protein controls in semi-quantitative immunoblotting. (1/4)

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Development of an affordable dye-stained microalbuminuria screening test. (2/4)

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Tandem scanning reflected-light microscopy of cell-substratum adhesions and stress fibres in Swiss 3T3 cells. (3/4)

This paper describes two applications of the tandem scanning reflected-light microscope (TSM) for the observation of the structure of individual cells growing in tissue culture. First, the TSM is used as an alternative to interference reflection microscopy (IRM) or total internal reflection aqueous fluorescence microscopy (TIRAF) to observe cell-substratum adhesions in unstained living cells growing on a glass coverslip. Second, the TSM is used to produce improved images of cellular structures in 3T3 cells stained with various protein dyes including Napthol Blue Black (NBB) and Coomassie Brilliant Blue (CBB). More specifically, close contacts and focal contacts are resolved in living 3T3 cells, and features of the nucleus, the cytoskeleton and extracellular matrix are resolved in both NBB- and CBB-stained cells. The focal contacts and associated stress fibres are clearly imaged in NBB-stained cells. The TSM is an improvement over conventional incident light microscopy because of the confocal image excludes information from out-of-focus regions of the cytoplasm, and, unlike the laser-based confocal microscope, the actual colour of the specimen is viewed directly with TSM in almost real-time.  (+info)

Remazol Brilliant Blue as a pre-stain for the immediate visualization of human serum proteins on polyacrylamide gel disc electrophoresis. (4/4)

We describe the pre-staining of proteins in normal human serum with Remazol Brilliant Blue before separation by disc electrophoresis. Critical to reproducibility are: dye concentration of 0.16 mol/L in a Tris-glycine buffer (pH 8.3), use of equal volumes of serum and dye solution, a tagging period of 2 h at room temperature, and electrophoresis of 0.1 mL of the mixture at 2.5 mA/gel for about 2 h. Advantages include speed, quality of resolution, and low background. This method was compared with Amido Black post-electrophoresis staining in 35 sera. Of these, 16 showed identical results with respect to the number of bands. In the other 19, Amido Black showed more bands in the post-transferrin region and, sometimes, in the post-albumin region. The pre-stained gels showed slower electrophoretic mobilities of the components. Protein bands eluted from pre-stained gels retained immunological reactivity.  (+info)

TY - JOUR. T1 - Chemical enhancement of footwear impressions in blood on fabric - part 1: protein stains. AU - Farrugia, Kevin J.. AU - Savage, Kathleen A.. AU - Bandey, Helen. AU - Nic Daéid, Niamh. PY - 2011/9. Y1 - 2011/9. N2 - A range of protein stains were utilised for the enhancement of footwear impressions on a variety of fabric types of different colours with blood as a contaminant. A semi-automated stamping device was used to deliver test impressions at a set force to minimise the variability between impressions; multiple impressions were produced and enhanced by each reagent to determine the repeatability of the enhancement. Results indicated that while most protein stains used in this study successfully enhanced impressions in blood on light coloured fabrics, background staining caused interference on natural fabrics. Enhancement on dark coloured fabrics was only achieved using fluorescent protein stains, as non-fluorescent protein stains provided poor contrast. A further comparison ...
Emir wrote: , , Dot blot your protein, same with the standard , at different concentrations, wash blots and apply amido black solution , (something like 0.1 g/100ml in 5% acetic acid, if I am not mistaken). The , visual comparison of the density of color allows you to estimate , concentration of the unknown protein. I guess you could develop such an , assay yourself without buying anything. No, the kit specifies that the *width* of the dot is used to determine the concentration, not the colour. If it had been colour I would have assumed amido black too. It also claims to be an absolute scale, with no standards required - the kit comes with a scaler to size the dots on and get the concentration from. The protocol is dilute, spot, fix, develop. Big surprise, theres no composition given in the on-line documentation for the dilution, fixative or developing buffers. love Anna -- I see you standing there, far out along the way, I want to touch you but, the night becomes the day. I count the words ...
1.FIX BLOOD IMPRESSION: Immerse in Methanol - 1 hour - or - Immerse in 5-Sulfosalicylic acid. 2.WORKING SOLUTION APPLICATION Squeeze bottle application of dye for 2 minutes. - or - Tray immersion of item into dye for 2 minutes. 3.RINSE SOLUTIONS Squeegee bottle used to apply rinse solution for 1 minute. - or - Tray immersion containing rinse, rock tray gently for 1 minute ...
P261:¯Avoid breathing dust/fume/gas/mist/vapours/spray.,P280:¯Wear protective gloves/protective clothing/eye protection/face protection.,P302 + P352:¯IF ON SKIN: Wash with plenty of soap and water.,P305 + P351 + P338:¯IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.. ...
Make Western blot normalization more accurate and reliable with REVERT Total Protein Stain. Provides a linear signal over a broad range of concentrations.
Thermo Scientific Imperial Protein Stain is a ready-to-use colorimetric stain formulated with coomassie dye R-250 that delivers consistent nanogram-level detection of... Shop
Reliable assessment of changes in target protein levels by western blot requires measurement of both the target and loading control proteins in the linear dynamic range. Stain-free technology is a novel method introduced by Bio-Rad to visualize and quantify proteins in gels and blots. Download this application note to learn how stain-free total protein measurement serves as a more reliable loading control than housekeeping proteins.
Upon quantifying band densities in ImageJ, I compared the reproducibility of p-Smad2 linker after normalization to all possible combinations of loading controls: 25 = 32 combinations. For single loading control normalization, the effect on replicate-to-replicate reproducibility heavily depended on the choice of loading control. Normalizing to GAPDH decreased the p-Smad2 linker coefficient of variation by more than twofold (21 to 9%), but normalizing to tubulin had virtually no effect (Fig. 6C). This does not imply that GAPDH is always a good loading control or that tubulin is always a bad one; rather, it emphasizes the danger of relying on a single measured variable to estimate total protein content. As higher-order combinations of loading controls were tested as normalizers, I found that the coefficient of variation of p-Smad2 linker steadily improved toward 7 to 8%, consistent with values reported previously (10, 11). This reproducibility became less dependent on the specific combination of ...
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ProSci Inc can help you find the perfect loading control antibody that can be customized to fit your project. Buy various antibodies online today from ProSci.
Rabbit polyclonal alpha Tubulin antibody - Loading Control. Validated in WB, ICC/IF and tested in Mouse, Rat, Chicken, Cow, Human, Chinese hamster. Cited in 263 publication(s). Independently reviewed…
Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) has been cited in 525 publications. References for Human, Mouse, Rat, Hrs, Chk, Cow, Pig, Mk…
引用Abcams Anti-Actin抗体- Loading Control (ab1801)的参考文献列表。为您列举引用本产品的发表文章,并提供信息包括论文文献数据库中的检索编号以便您搜寻文章
Anti-GAPDH antibody - Loading Control (HRP) (ab9385) has been cited in 46 publications. References for Human, Mouse, Rat, Cow, Sc in WB
Anti-GAPDH antibody - Loading Control (ab9485) has been cited in 527 publications. References for Human, Mouse, Rat, Rabbit, Chk, Dog, Pig, RMk in ICC, ICC/IF…
TY - JOUR. T1 - Unsuitable of using ribosomal RNA as loading control fot northen blot analyses related to the imbalance between messenger and ribosomal RNA content in rat mammary tumors. AU - Solanas, M.. AU - Moral, R.. AU - Escrich, E.. PY - 2001/1/1. Y1 - 2001/1/1. M3 - Article. VL - 288. SP - 99. EP - 102. ER - ...
Orochem offers a wide variety of total protein stains for in-gel protein detection and visualization. A wide selection of stains developed at Orochem varies in sensitivity, staining time and reversibility.
The invention relates to the staining of poly(amino acids), including peptides, polypeptides and proteins in gels and on solid supports, using neutral or anionic complexes of transition metals.
TY - JOUR. T1 - Western blotting using in-gel protein labeling as a normalization control. T2 - Stain-free technology. AU - Gilda, Jennifer E.. AU - Gomes, Aldrin V. PY - 2014/12/4. Y1 - 2014/12/4. N2 - Western blotting is a commonly used laboratory technique for semi-quantifying protein amounts. It is important when quantifying protein expression to account for differences in the amount of total protein loaded onto the gel using a loading control. Common loading controls include housekeeping proteins, such as β-actin or GAPDH, quantified by Western blot, or total protein, quantified using a stain such as Coomassie Brilliant Blue or Ponceau S. A more recently developed method for total protein quantification utilizes stain-free technology, which has a linear dynamic detection range and allows for protein detection on both gels and membranes. Here, we describe the theory and use of stain-free gels for total protein quantification and normalization of Western blots.. AB - Western blotting is a ...
Fig. 4. LAMP-1 antibody-based studies. A, Western blot analysis of normal and stage-specific tissue lysates. LAMP-1 is detected at ∼110 kDa. 10 μg total protein lysates loaded. Protein levels were normalized to actin loading control. Aconitase 2 is detected at ∼80 kDa. Protein levels were normalized to tubulin loading control. B-K, immunohistochemical analysis of normal and cancerous ovarian tissue. LAMP-1 is not stained in normal ovarian tissue sections (B-E). Sections stained with mouse IgG as a negative control. LAMP-1 is expressed in serous epithelial ovarian adenocarcinoma tissue sections (G-J). LAMP-1 expression is specifically observed in the cytoplasm of epithelial cells. Absence of the LAMP-1 specific staining when probed with mouse immunoglobulin as a negative control (K). ...
Figure 4: Curcumin attenuates doxorubicin cardiotoxicity via regulation of autophagy and apoptosis through JNK phosphorylation. (A) Representative western blot analysis of pJNK, JNK, in post nuclear supernatant of neonatal rat cardiomyocyte. Upper number means loading time of each treatment. Data were normalized with Tubulin which serves as a loading control. Each bar represents mean ± SEM of four independent experiments. *p,0.01, #p,0.05. (B) Representative confocal photomicrograph of cardiomyocyte transfected with GFP-LC3 treated with starvation, DOX, Cur, DOX+Cur over 2 h respectively. Lower tier cells were treated by SP600125 (JNK inhibitor) 1 h prior to each treatment. (C) Representative western blot analysis of LC3 in neonatal rat cardiomyocyte with JNK inhibitor 1 h prior to each treatment over 2 h. β-actin serves as a loading control. Each bar represents mean ± SEM of four independent experiments. *p,0.01. (D) Representative western blot analysis to detect cleaved PARP, pJNK, JNK in ...
FIGURE 3. IFN-β reduces cell migration through IFIT2 expression. A. Enhanced expression of IFIT2 following IFN-β treatment. Logarithmically growing OC3 cells were treated with various concentrations of IFN-β for 24 h. RT-PCR (left) and Western blot (right) analysis of IFIT2 in OC3 cells treated with various doses of IFN-β (0-500 units/mL). At the end of treatment, the amount of IFIT2 induced by IFN-β was analyzed by RT-PCR (GAPDH was used as the loading control) and Western blotting (β-actin was used as the loading control). All experiments were done thrice, and one representative experiment is shown. B. Antiproliferation effect of IFN-β on OC3 cells. Cells were treated with various concentrations of IFN-β for 24 or 48 h. Cells were counted and examined for viability by trypan blue dye exclusion. Points, average of three independent experiments; bars, SD. C. Migration inhibition of IFN-β on OC3 cells. Cells were seeded in the upper chamber of a Transwell cell culture system, and after ...
Western blot, a method to separate proteins by size and analyse their relative expression levels, is a much maligned technique of molecular cell biology. The website PubPeer is flooded with evidence of manipulated Western blots, where gel lanes were inappropriately spliced, or where bands digitally duplicated or erased. Some even question the technology as such,…
Western blot, a method to separate proteins by size and analyse their relative expression levels, is a much maligned technique of molecular cell biology. The website PubPeer is flooded with evidence of manipulated Western blots, where gel lanes were inappropriately spliced, or where bands digitally duplicated or erased. Some even question the technology as such,…
When Western Blot or other experiments are performed, loading controls are required to ensure that (1) the same amount of protein sample is loaded into each lane, (2) protein is transferred from the gel to membrane with equal efficiency across the whole membrane, and (3) antibody incubation and detection is uniform throughout the experimental procedure. Loading controls must fulfill certain criteria before they are chosen as normalization factor to the protein changes in samples. Released July 2015.. Download. ...
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The two tables below display ChEMBL single-protein targets which are predicted to interact with CHEMBL25. A 1uM and 10 uM cut-off have been applied to ChEMBL bioactivity data used to generate the respective models and the green coloured rows correspond to genuine predictions, i.e. targets not included in the original training set for this compound ...
The two tables below display ChEMBL single-protein targets which are predicted to interact with CHEMBL25. A 1uM and 10 uM cut-off have been applied to ChEMBL bioactivity data used to generate the respective models and the green coloured rows correspond to genuine predictions, i.e. targets not included in the original training set for this compound ...
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How to Prepare Chicken and Rice for Dogs. Homemade chicken and rice is a bland food source often recommended by veterinarians to help dogs recover from diarrhea and/or vomiting. It is an easily digestible low-fat, single-protein, and...
Revert Destaining Solution is for use with the Revert 700 Total Protein Stain. It consists of 0.1 M sodium hydroxide, 30% (v/v) methanol, in water. This product requires additional fees for hazardous shipping costs.. This product is sufficient for 20 mini Western blots (10 mL/blot).. ...
O pinhão é a semente comestível da Araucaria angustifolia, sendo consumido no sul e sudeste do Brasil principalmente na sua forma in natura. A falta de diversidade em relação ao seu consumo acarreta a sobra da safra e a sua utilização como ração animal, o que diminui o valor de comercialização do produto, prejudicando os ganhos econômicos do produto. A semente do pinhão é composta de 34% de amido e um conteúdo muito baixo de proteínas e lipídios, o que a torna favorável para a obtenção de amido, aditivo amplamente utilizado pela indústria de alimentos. O objetivo deste trabalho foi caracterizar uma nova fonte de amido a partir de semente de Araucária angustifolia, através de um estudo detalhado da metodologia de extração do amido de pinhão, das características físico-químicas, funcionais e reológicas do grânulo e de sua modificação estrutural. Primeiramente foi determinado um protocolo para a extração do amido e realizado o estudo do efeito do tratamento ...
A cause of bad breath is protein stains on your teeth. Using Propolinse mouthwash, you can see the protein stains wash away in your mouth thanks to a special formula. Your mouth will feel refreshed and clean after use. The mouthwash protects against germs that cause bad breath. How to Use: Gargle half a cap-full for 20
Fig. 2C -STAT 3 loading control is actually the b-acting loading control from Fig. 4B of 20103603. (3 years apart, and hes using the same image for 2 totally different things. Also, theres evidence of material being erased from the top-right corner of the image on both cases - thats how we know they are the same!) ...
Fig. 2C -STAT 3 loading control is actually the b-acting loading control from Fig. 4B of 20103603. (3 years apart, and hes using the same image for 2 totally different things. Also, theres evidence of material being erased from the top-right corner of the image on both cases - thats how we know they are the same!) ...
5 Degree Spot (Optional) The Sekonic 5 Degree Spot Viewfinder for the LITEMASTER PRO L-478D Series of light meters is an attachment spot viewfinder that features a 5° metering area for more precise, reflected-light measurment.Built In PocketWizard PocketWizard triggering and Flash Power Setting w/ControlTL radios. Control Nikon, Canon and select studio flashes with a touch of a fingerFull Featured Meter For CinematographersDial in your exact shutter angle and frame rate to get exact exposures, and evaluate the lighting in your scene.Full Featured Meter For CinematographersDial in your exact shutter angle and frame rate to get exact exposures, and evaluate the lighting in your scene.Whats in the Box• Sekonic L-478DR-U LITEMASTER PRO for PocketWizard System Light Meter• Anti-Glare Sheet for LCD Screen• Lens Cap• Strap• Soft Case• Limited 1-Year Warranty
In detail, we demonstrated the capacity of the combined approach to target the CSCs compartment in mCRPC models and unveiled a novel molecular mechanism underlying this synergism based on the inhibition of the oncogene YAP activity. in 22Rv1 in cell adhesion condition, in 1st generation spheres and in 2nd generation spheres. -actin was used as housekeeping control gene to normalize RT-PCR reactions. C. Basal expression of NANOg evaluated by western blotting in 22Rv1 cells in cell adhesion condition, in 1st generation spheres and in 2nd generation spheres. tubulin was used as loading control. D. Surface marker expression (CD44 and CD113) was determined by circulation cytometry on 22Rv1cells at basal level in 22Rv1 in both cell adhesion condition and 1st generation spheres. E. CTGF mRNA expression evaluated by RT-PCR at basal level in Noscapine 22Rv1 in both cell adhesion condition and in 1st generation spheres. -actin was used as housekeeping control gene to normalize RT-PCR reactions ...
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a) pRB was induced by tetracycline in a stably transfected SAOS2 cell line (SAOS-tetRB) and protein samples for immunoblotting were harvested at various times after pRB induction. GAPDH, loading control. Uncropped images of the gels are shown in the Supplementary Information, Fig. S6e. (b) pRB-mediated G1 arrest of corresponding samples was monitored by FACS counting BrdU-incorporating S-phase cells after tetracycline treatment of parental SAOS2 cells that do not carry inducible pRB (parental, grey bars), and of pRB-inducible SAOS2 as in a (tet-pRB). (c) pRB-APCcdh1 association was monitored over time by immunoprecipitation using anti-pRB antibodies. The pRB antibody concentration was limiting with regard to extract to obtain equal quantities of immunoprecipitated pRB at the different times. Ig: heavy chain. Uncropped images of the gels are shown in the Supplementary Information, Fig. S6f. (d, e) As controls for the specificity of APCcdh1- and pRB-mediated Skp2 protein turnover, SAOS-tetRB cells ...
Fig. 6. WDR34 is required for ciliogenesis. Cells were transfected with siRNA duplexes to suppress WDR34. (A-C) Silencing of WDR34 (red) causes a defect in ciliogenesis (acetylated tubulin labelling, green). Boxed regions show enlargements. Note that in C, the lower inset illustrates that a robust loss of WDR34 correlates with a failure to form cilia. The upper box shows that those cells that are less effectively suppressed still retain the ability to form primary cilia and that these remaining cilia are longer than controls (compare with A). (D) Immunoblotting of WDR34-depleted cells and lamin-A/C-depleted control, α-tubulin is used as a loading control. (E) Quantification of the ciliogenesis defect on WDR34 suppression. *P,0.01 using ANOVA with Dunnetts post-hoc test. Error bars represent s.d. (F) As in C, remaining cilia in WDR34-depleted cells were significantly longer than in control cells. (G) The typical pericentrosomal accumulation of WDR34 seen in control cells (arrows) is lost on ...
Figure 1. Cdh1 phosphorylation at Cdk sites promotes Cdh1 stability. A, Schematic of nine conserved sites of potential Cdk phosphorylation in Cdh1 (S/T-P), numbered according to human Cdh1. B, Lysates of 293T cells transfected with GFP-Cdh1 WT, 9A, or 9D were immunoblotted using a polyclonal antibody to Cdh1 or Erk, the latter to serve as a loading control. Quantitation of Cdh1 levels normalized by Erk revealed that the levels of the 9D and 9A mutant proteins were, respectively, increased by 122% and reduced by 57% relative to wild-type Cdh1 in 293T cells (average of 4 experiments). C, Lysates of Neuro2A cells transfected with GFP-Cdh1 WT, 9A, or 9D and treated with MG132 (5 μm) or the vehicle control DMSO were immunoblotted for GFP or Erk. The levels of the 9D and 9A mutant proteins were, respectively, increased by 150% and reduced by 37% relative to wild-type Cdh1 in Neuro2A cells (average of 2 experiments). MG132 treatment, respectively, increased WT, 9A, and 9D levels by 80, 134, and 9%. D, ...
Fig. 3. Glucose is required for the PM localization of K-RasG12V and LactC2 in cells overexpressing ATP5G1(1-67). (A) Cell lysates of MDCK cells stably co-expressing mGFP-K-RasG12V or mGFP-H-RasG12V with RFP alone (−) or ATP5G1(1-67)-RFP (+) were immunoblotted for phospho-ERK, phospho-Akt (S473), and mGFP-RasG12V, and quantified (mean±s.e.m.) from three independent experiments. Significant differences between cells not expressing (−) or expressing (+) ATP5G1(1-67) were assessed using Students t-test (*P,0.05; ***P,0.001). Representative blots of are shown, with total ERK, total Akt and actin used as loading controls. (B) MDCK cells stably expressing mGFP-K-RasG12V in the presence (+) or absence (−) of ATP5G1(1-67)-RFP were maintained in complete growth medium (DMEM+10% FBS+2 mM L-glutamine). When cells reached full confluence, the growth medium was replaced with fresh complete growth medium and cells were further incubated for indicated time points. Cells were fixed with 4% PFA and ...
Figure 7. siRNA-mediated depletion of 4E-T affects mRNA turnover. HeLa cells were transfected with siRNA against 4E-T or a control (Ctrl) siRNA (4AIII inverted). Protein and RNA were harvested for use in Western (C and D) and Northern (A, B, and E) analysis, respectively. (A and B) HeLa cells were transfected with siRNA against 4E-T or with control siRNA and later cotransfected with pTet-β-globin or pTet-β-globin c-fos or GM-CSF ARE and pTet Off. Cells were treated with doxycycline (1 μg/ml) ∼72 h after transfection to block transcription. Total RNA was isolated at 0.5, 2, 3, and 4 h for β-globin/GM-CSF ARE assays (A) and 0.5, 1.5, 3, and 6 h for β-globin/c-fos ARE assays (B) and analyzed by Northern blot. GAPDH served as a loading control. mRNA half-lives were calculated from Northern blots and normalized against GAPDH levels. (C) Protein extracts were collected at 6 h after treatment with doxycycline (1 μg/ml) from cells that were transfected with the reporter plasmid and pTetOff and ...
Fig. 4B - this is a b-actin blot. It is IDENTICAL to a STAT3 loading control used in Figure 2C of PMID 17505005 (Clin Cancer Res. 2007 May 15;13(10):3024-32.). Something is erased from the top right coner in both images, so we know theyre the same. ...
Figure and Images: Expression of functional MMP-9 by GLV-1h255-infected tumor cells. (A) Expression cassettes of GLV-1h68 and GLV-1h255. In GLV-1h255 the insert in the Tk locus was replaced by the human mmp-9 gene under control of the PSE promoter. PSEL, synthetic early/late promoter; PSE, synthetic early promoter; P7.5, VACV p7.5 K early/late promoter; P11, VACV p11 late promoter; Tk, thymidine kinase locus, Ha, hemagglutinin locus. (B) Expression of virus-encoded MMP-9 (92 kDa) in GLV-1h255 infected PC-3 cells and supernatants in vitro, β-actin (42 kDa) was used as a loading control. (C) Activity of the MMP-9 protein was tested by gelatin zymography. Lysates and supernatants of infected A549 cells were isolated and separated by non-reducing SDS-PAGE. In zymography, cleavage of the substrate by MMP-9 resulted in a clear band. ...
Images: MPZ and PMP22 expression in the sciatic nerve of WT and Nox4−/− mice after SNI. A, Western blot analysis of the myelin-specific proteins MPZ and PMP22 in the day 14 SNI sciatic nerve (proximal nerve stump) and the uninjured control sciatic nerve. GAPDH was used as loading control. Note that MPZ and PMP22 protein expression is significantly decreased after SNI in WT mice but not in Nox4−/− mice. n = 3 mice per group. Data are presented as mean ± SEM (*p , 0.05). B, Immunostaining of the day 14 SNI sciatic nerve shows increased MPZ immunoreactivity in Nox4−/− mice compared with WT mice, whereas immunoreactivity of the neuronal marker NF200 is similar in both genotypes. Scale bar, 10 μm. ...
Neutral cobalt(II) complexes of the dianionic tetrapodal pentadentate ligand B2Pz4Py, in which borate linkers supply the anionic charges, are reported. Both the six-coordinate THF adduct 1-THF and the five-coordinate THF-free complex 1 are in a high-spin S = 3/2 configuration in the ground state and have been structurally characterized by X-ray crystallography. These two Co(II) starting materials react rapidly with aryl azides of moderate steric bulk. The thermodynamic products of these reactions are low-spin, diamagnetic, Co(III) amido complexes that are either monomeric, when an external hydrogen atom source such as 1,4-cyclohexadiene is present, or dimeric products formed via C-C coupling of the azide aryl group and internal transfer of H• to the nitrogen. These products are fully characterized and are rare examples of octahedral Co amido compounds; structural determinations reveal significant pyramidalization of the amido nitrogens due to π-π repulsion wherein the amido ligand is ...
N-[2-(Substituted amido)phenyl]-N,N-bis-carbonylguanidines bearing an optionally substituted phenoxy, phenylthio, phenylsulfinyl or phenylsulfonyl group in the 4- or 5-position of the 2-(substituted amido)phenyl group are anthelmintic agents. The compounds, of which N-(2-acetamido-4-phenylthiophenyl)-N,N-bis-carbomethoxyguanidine is a typical example, are prepared through the reaction of isothiourea-S-alkyl ether and an appropriately substituted 2-aminoanilide.
Bis[bis(trimethylsilyl)amido]manganese(II) Mn(C6H18NSi2)2 bulk & research qty manufacturer. Properties, SDS, Applications, Price. Free samples program. Term contracts & credit cards/PayPal accepted.
4HVG: 3-Amido Pyrrolopyrazine JAK Kinase Inhibitors: Development of a JAK3 vs JAK1 Selective Inhibitor and Evaluation in Cellular and in Vivo Models.
They are visualised using Napthal Black or Amido Black staining. Typical starch gel concentrations are 5% to 10%. Denaturing ...
Examples include: amaranth dye amido black armstrong's acid congo red evans blue suramin trypan blue Booth, Gerald (2005). " ...
Wilson CM (1979). "Studies and critique of Amido Black 10B, Coomassie Blue R, and Fast Green FCF as stains for proteins after ... Amido black 10B staining, Fast green FCF staining, fluorescent stains such as epicocconone stain and SYPRO orange stain, and ...
Ultraviolet-visible spectroscopy Biuret protein assay Lowry protein assay BCA protein assay Amido black protein assay Ninfa, ...
Amido black is a commonly used permanent post-antibody anionic stain that is more sensitive than Ponceau S. This stain is ...
... although they may result in the binding of anionic dyes such as Coomassie blue and Amido black. Polyvinylidene fluoride ...
... such as amido black, and indigo carmine. Copper is one of the most critically important of the trace minerals that are ... It was found in fumarolic deposits, and a weathering product of sulfides in subsea black smoker deposits. It was named for the ...
Other dyes used in connection with Van Gieson staining include: Alcian blue Amido black 10B Verhoeff's stain Jocelyn H. Bruce- ...
Amido Black, and Cy5. In order to avoid noise of signal, total protein staining should be performed before blocking of the ...
... commonly with Amido Black or Coomassie Brilliant Blue), and areas of digestion appear as clear bands against a darkly stained ...
... amido black MeSH D04.615.638.555.282 - anilino naphthalenesulfonates MeSH D04.615.638.555.300 - congo red MeSH D04.615.638.555. ...
It stains the proteins in blood a blue-black color. Amido Black can be either methanol or water based as it readily dissolves ... Amido black 10B is an amino acid staining azo dye used in biochemical research to stain for total protein on transferred ... Western blot normalization "AMIDO BLACK 10B". chemicalland21.com. Retrieved December 2, 2017. CS1 maint: discouraged parameter ...
Amido black 10B (naphthol blue black). *Bafilomycin A1. *Benzopurpurin 4B. *Bumetamide. *Chicago sky blue 6B ... were the lower-quality brands containing black tea, with a supermarket brand of black tea having the highest theanine content. ... Theanine is substantially present in black, green, and white teas from Camellia sinensis in quantities of about 1% of the dry ...
Amido black 10B (naphthol blue black). *Bafilomycin A1. *Benzopurpurin 4B. *Bumetamide. *Chicago sky blue 6B ...
Amido black 10B (naphthol blue black). *Bafilomycin A1. *Benzopurpurin 4B. *Bumetamide. *Chicago sky blue 6B ...
Amido black 10B (naphthol blue black). *Bafilomycin A1. *Benzopurpurin 4B. *Bumetamide. *Chicago sky blue 6B ...
Atwater, J.E., and Wheeler, R.R., Jr., Temperature Dependent Complex Permittivities of Graphitized Carbon Blacks at Microwave ... Atwater, J.E., and Akse, J.R., Cobalt - Poly(amido amine) Superparamagnetic Nanocomposites, Materials Letters 62, 3131-3134, ...
... is a dye of blue, bluish-red or black shades. Induline consists of a mixture of several intensely colored species, so ... The indulines are prepared as mentioned above from aminoazo compounds, or by condensing oxy- and amido-quinones with phenylated ...
Its internal systematics is confusing; the plumage is usually dull, with shades of black, brown, and gray; from their outward ... including amido nitrogen, monoamine nitrogen, non-amino nitrogen, arginine, humin, histidine, lysine and cysteine), 30.6 g ... Aerodramus sawtelli, the Atiu swiftlet, and Aerodramus maximus, the black-nest swiftlet are the only known species which emit ... and the black-nest swiftlet. Instead of twigs, feathers and straw, these swiftlets make their nest only from strands of their ...
Celtic won the match 1-0 thanks to a goal from Amido Balde. There were talks ongoing about a return of the 'Dublin Decider' in ... "Ireland 22-24 New Zealand: Last-gasp All Blacks break Irish hearts". Scrum. ESPN Sports Media. 24 November 2013. Retrieved 24 ...
On the other hand, the appearance of a black mirror deposit in the cool part of the equipment indicates the presence of ... Amido, Phosphido, and Arsenido Derivatives of aluminum and gallium. X-ray Structures of [tert-Bu2Ga(m-OH)]3 and [tert-Bu2Ga(m- ... With solid AgNO3, AsH3 reacts to produce yellow Ag4AsNO3, whereas AsH3 reacts with a solution of AgNO3 to give black Ag3As. The ...
... the Semnani people despise the color black because of its utilization for the black flags of the Abbasid Caliphate. The Abbasid ... Amid os-Saltaneh (1875-unknown) - physician and politician Mirza Javad Khan Ameri (1891-1980) - politician Maryam Amid Semnani ... It has also been reported that the people of Semnan have historically refused to wear black clothing for the mourning of the ... refusal appears to be rooted in the hatred of the people of Semnan towards the Abbasid Caliphate and its utilization of black ...
"Celtic striker Amido Baldé gets to show his skills for one season at the Freethiel" (in Dutch). waasland-beveren.be. 2014-09-01 ... "Young promising goalkeeper Thomas De Bie (KV Mechelen) strengthens green-black squad" (in Dutch). cerclebrugge.be. 2014-04-17 ...
It stains the proteins in blood a blue-black color. Amido Black can be either methanol or water based as it readily dissolves ... Amido black 10B is an amino acid staining azo dye used in biochemical research to stain for total protein on transferred ... Western blot normalization "AMIDO BLACK 10B". chemicalland21.com. Retrieved December 2, 2017. CS1 maint: discouraged parameter ...
Join researchers using high quality Naphthol Blue Black from Abcam and… ... Buy Naphthol Blue Black (CAS 1064-48-8), a water soluble histological and protein dye. ... Decolorization of naphthol blue black using the horseradish peroxidase.. Appl Biochem Biotechnol 163:433-43 (2011). Read more ( ...
CAUTION! MAY BE HARMFUL IF SWALLOWED OR INHALED. MAY CAUSE IRRITATION SKIN, EYES, AND RESPIRATORY TRACT. THE TOXICOLOGICAL PROPERTIES OF THIS SUBSTANCE HAVE NOT BEEN FULLY INVESTIGATED ...
You can purchase Amido Black powder (25 gram bottle) and mix it in Glacial Acetic Acid and Methanol.Shelf-life of Amido Black ... Lightning Powder 1-0046 25g Amido Black Amido Black is a protein dye sensitive to the properties in blood. It can be used to ... Home > Lightning Powder > Investigations > Fingerprints > 1-0046 25g Amido Black. Lightning Powder 1-0046 25g Amido Black. ... 1-0046 25g Amido Black. Amido Black is a protein dye sensitive to the properties in blood. It can be used to enhance or develop ...
Amido Black reacts with blood proteins to create a deep blue color change. This formulation includes a blood fixative in the ... Amido Black is an effective blood reagent when latent or difficult to see blood stains are present. ... Amido Black Water-Based Kit, 32 oz.. MODEL: SKU: PART: Amido Black is an effective blood reagent when latent or difficult to ... Amido Black is an effective blood reagent when latent or difficult to see blood stains are present. Amido Black reacts with ...
Naphthol Blue Black. Naphthalene 12B. Acid Black 1, 10A, 10B. Acid Black 12B, 10BA. Eriosin Blue Black B. Acidal Black 10B. ... Amido Black ***. ,. Forensic Light. ,. Physical Developer. AMIDO BLACK - Water Base. FORMULA:. 1st SOLUTION : (Citric Acid ... AMIDO BLACK - Water Base. Surface(s) Used On:. Blood stained non-porous surfaces, especially plastics.. Sensitive To:. Blood ... AMIDO BLACK - Water Base. [ Return to: Main Page ,, Non-Porous Surfaces ,, Glass Surfaces ,, Plastic Bag Surfaces ,, Blood ...
... in Methanol as in the procedure for the mixed solution of the Amido Black powder above.Shelf-life of Aqueous Amido Black: one ... 1-2741 Aqueous Amido Black Aqueous Amido Black is a pre-mixed Amido Black solution. It can be used to enhance or develop blood- ... 1-2740, 1-2741 Aqueous Amido Black. Aqueous Amido Black is a pre-mixed Amido Black solution. It can be used to enhance or ... 1-2741 Aqueous Amido Black. Lightning Powder 1-2740, 1-2741 Aqueous Amido Black. Option:. Choose Your Option 1 Liter. 1 Gallon ...
J66798 Amido Black 10B, Electrophoresis Reagent Amido Black 10B can be used to stain proteins on polyacrylamide gels, agarose ... The gel can then be stained with 0.1% Amido Black 10B in a 7% (v/v) solution of acetic acid for at least 2 hours. Then de-stain ... The detection sensitivity of Amido Black 10B is approximately 20% that of Coomassie Blue R. ...
Although it will not detect the typical chemical components of latent prints, Amido Black is very sensitive ... Amido Black is useful for enhancing latent prints contaminated with blood, but it will not successfully develop a normal ... Amido Black is very sensitive to the proteins found in blood and some other body fluids. It leaves a blue-black stain and is ... Amido Black is useful for enhancing latent prints contaminated with blood, but it will not successfully develop a normal ...
Amido Black Stain is a dye that stains the protein component of blood and cerebrospinal fluid blue black. It is also used to ...
P261:¯Avoid breathing dust/fume/gas/mist/vapours/spray.,P280:¯Wear protective gloves/protective clothing/eye protection/face protection.,P302 + P352:¯IF ON SKIN: Wash with plenty of soap and water.,P305 + P351 + P338:¯IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.. ...
... supplier and exporter of AMIDO BLACK 10B (For Molecular Biology) at reasonable price. ... AMIDO BLACK 10B (For Molecular Biology) AMIDO BLACK 10B (For Molecular Biology) ...
View our list of indicators and dyes used to determine pH or presence of metal ions. Purchase indicators and dyes at Alfa Aesar online.
Protein Quantification Using Amido Black Assay (X1). For quantification of protein concentration (Supplementary Data Sheet S1) ... 50 μL of the sample were precipitated with 300 μL amido black staining solution. Afterward, the sample was centrifuged (5 min, ... The samples BGP, human gut, soil, and compost, and WWTP as well as their averages (black line) are shown separately. For ...
Amido black bioWORLD. Stain for detection and location of proteins. Protocol: Wash blot in 0.#% Tween/PBS, with three changes ...
Known protein stains, such as Amido Black and CBB staining, are difficult to destain, prevent subsequent immunostaining, and ... Proteins appear as white bands on a gray to black background or as black bands on light gray to white background on the ... Proteins appear as white bands on a gray to black background or as black bands on light gray to white background on the ... The stained proteins in the gel are photographed using Polaroid 667 black and white print film using a Kodak #Wratten 9 gelatin ...
Amido Black is useful for enhancing latent prints contaminated with blood, however it will not successfully develop a normal ... Youre reviewing: Amido Black - Powder - 25 gm. How do you rate this product? *. 1 star. 2 stars. 3 stars. 4 stars. 5 stars. ... Amido black is an effective blood reagent when latent or difficult to see blood stains are present. AB will develop exceptional ... Amido black is an effective blood reagent when latent or difficult to see blood stains are present. AB will develop exceptional ...
Amido Black staining Is the Subject Area "Amido Black staining" applicable to this article? Yes. No. ...
... they did an Amido Black test. So Amido Black reacts to protein. Proteins found in blood. So as they sprayed the stuff on the ... And that pool is exactly where Annie laid, and she bled out," Agent Jim Grebas explained of the Amido Black test. NCIS Agent ...
Kinetics and mechanism of oxidation of amido black by sodium N-halo-p-toluenesulfonamides in acidic medium: Spectrophotometric ... Shubha, J., Vinutha, K., & Puttaswamy, P. (2013). Kinetics and mechanism of oxidation of amido black by sodium N-halo-p- ... Shubha, J.; Vinutha, K.; Puttaswamy, P. Kinetics and mechanism of oxidation of amido black by sodium N-halo-p- ... "Kinetics and mechanism of oxidation of amido black by sodium N-halo-p-toluenesulfonamides in acidic medium: Spectrophotometric ...
Naphthol blue black) by chloramine-T (CAT) and bromamine-T (BAT) in acidic medium has been investigated spectrophotometrically ... KEYWORDSThe kinetics of oxidative decolorization of amido black (AB, ... KEYWORDSThe kinetics of oxidative decolorization of amido black (AB, Naphthol blue black) by chloramine-T (CAT) and bromamine-T ... Kinetics and mechanism of oxidation of amido black by sodium N-halo-p-toluenesulfonamides in acidic medium: Spectrophotometric ...
Amido Black Spray 8 oz SKU: LV501L $10.51 Quick Look. Add to Cart. ...
Naphthol Blue Black C22H14N6O9S2Na2 bulk & research qty manufacturer. Properties, SDS, Applications, Price. Free samples ... Naphthol Blue Black Synonyms. Amido Black; Amido Black 10B; Acid Black 1; Buffalo Black NBR; Disodium 4-amino-5-hydroxy-3-[(4- ... About Naphthol Blue Black. Naphthol Blue Black is generally immediately available in most volumes. High purity, submicron and ... Naphthol Blue Black Properties (Theoretical). Compound Formula. C22H14N6O9S2Na2. ...
Samples were incubated in the gluten or mucin-agar plated for 14 h. Amido black was used to stain protein in the mucin bioassay ... Then, 1.5 × 104 cells were plated into each well of a 96-well black plate for 24 h. The cell monolayers were then washed three ...
FIGURE S1 , Amido black staining at 0, 14, and 42 DAG in the megagametophyte sections. Amido black stains total proteins. They ... To identify starch and proteins, sections were stained with Lugol solution (Biopack 151205, Argentina) and Amido Black (Anedra ... The black asterisk indicates plasmolysis, the double white asterisk indicates crushed cells, and the black arrow indicates ... Vacuolar proteins were stained with Amido black at 0 DAG. Storage proteins were relatively scarce and diluted in the water of ...
for use with Amido black for use with Colloidal gold for use with Coomassie brilliant blue ...
for use with Amido black for use with CPTS for use with Colloidal gold ...
ATF-LS-LP 8 Amido Black (253.2 KB) Amido Black or naphthalene black 10B is a protein indicator particularly sensitive to those ... ATF-LS-LP21 Sudan Black (261.27 KB) Sudan Black is a dye which stains fatty components of sebaceous sweat to produce a blue- ...
  • It stains the proteins in blood a blue-black color. (wikipedia.org)
  • Amido Black is an effective blood reagent when latent or difficult to see blood stains are present. (safariland.com)
  • Amido Black Stain is a dye that stains the protein component of blood and cerebrospinal fluid blue black. (prinsep.com)
  • Sudan Black is a dye which stains fatty components of sebaceous sweat to produce a blue-black image. (atf.gov)
  • The detection sensitivity of Amido Black 10B is approximately 20% that of Coomassie Blue R. (alfa.com)
  • The protein can be stained with Coomassie Blue R-250, Amido black, Ponceau S or Sypro Ruby. (edu.au)
  • The staining of the transferred proteins can easily be done with standard methods like Ponceau S, Coomassie Blue or Amido Black. (sartorius.com)
  • Amido black 10B is an amino acid staining azo dye used in biochemical research to stain for total protein on transferred membrane blots, such as the western blot. (wikipedia.org)
  • Amido Black 10B can be used to stain proteins on polyacrylamide gels, agarose gels and nitrocellulose membranes. (alfa.com)
  • It leaves a blue-black stain and is usable on both porous and nonporous surfaces. (sirchie.com)
  • Nonporous surfaces tend to yield the best results with Amido black and require little or no over stain rinsing. (arrowheadforensics.com)
  • The SAPs were also used to adsorb three other dyes, namely, Congo red, Amido black and Alizarin cyanine green. (ias.ac.in)
  • Amido Black is useful for enhancing latent prints contaminated with blood, but it will not successfully develop a normal perspiration-based print. (sirchie.com)
  • Although it will not detect the typical chemical components of latent prints, Amido Black is very sensitive to the proteins found in blood and some other body fluids. (sirchie.com)
  • Amido Black reacts with blood proteins to create a deep blue color change. (safariland.com)
  • Amido Black or naphthalene black 10B is a protein indicator particularly sensitive to those proteins present in blood. (atf.gov)
  • The membrane is stained with amido black for visualization of proteins, dried and packaged under anoxic conditions. (novusbio.com)
  • Amido Black can be either methanol or water based as it readily dissolves in both. (wikipedia.org)
  • Sigma-Aldrich MSDS] See "Amido Black. (nih.gov)
  • 1st SOLUTION : (Citric Acid Stock) Citric Acid 38 g Distilled water 2000 ml 2nd SOLUTION : (Developer Solution) Citric Acid Stock 1000 ml Amido black powder 2 g Kodak 'PhotoFlo 600' solution 2 ml 3rd SOLUTION : (1st. (cbdiai.org)
  • Lightning Powder 1-2740, 1-2741 Aqueous Amido Black FREE SHIPPING! (copsplus.com)
  • The gel can then be stained with 0.1% Amido Black 10B in a 7% (v/v) solution of acetic acid for at least 2 hours. (alfa.com)
  • Decolorization of naphthol blue black using the horseradish peroxidase. (abcam.com)
  • Naphthol Blue Black is generally immediately available in most volumes. (americanelements.com)
  • The membrane was stained with Amido Black to show loading. (g3journal.org)
  • SDS-Page: INSTA-Blot Multi-species Liver, Lung, Spleen Tissues [NBP2-30117] - Approximately 20 ug per lane of tissue lysates are resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. (novusbio.com)
  • Condition: Used, VIN: 000KP667, Mileage: 0, Engine: 1250 CC 4-Cylinder, Exterior Color: Black, Interior Color: Black, Seller Notes: 1952 MG TD Roadster For Sale in Marietta, Georgia 30067 Take a stroll down memory lane with this 1952 MG TD Roadster! (claz.org)