Amido Black: A dye used to stain proteins in electrophoretic techniques. It is used interchangeably with its acid form.Mouthwashes: Solutions for rinsing the mouth, possessing cleansing, germicidal, or palliative properties. (From Boucher's Clinical Dental Terminology, 4th ed)Crown Ethers: Macrocyclic polyethers with the repeating unit of (-CH2-CH2-O)n where n is greater than 2 and some oxygens may be replaced by nitrogen, sulfur or phosphorus. These compounds are useful for coordinating CATIONS. The nomenclature uses a prefix to indicate the size of the ring and a suffix for the number of heteroatoms.Salicylates: The salts or esters of salicylic acids, or salicylate esters of an organic acid. Some of these have analgesic, antipyretic, and anti-inflammatory activities by inhibiting prostaglandin synthesis.Immersion: The placing of a body or a part thereof into a liquid.Benzenesulfonates: Organic salts and esters of benzenesulfonic acid.Dental Impression Technique: Procedure of producing an imprint or negative likeness of the teeth and/or edentulous areas. Impressions are made in plastic material which becomes hardened or set while in contact with the tissue. They are later filled with plaster of Paris or artificial stone to produce a facsimile of the oral structures present. Impressions may be made of a full complement of teeth, of areas where some teeth have been removed, or in a mouth from which all teeth have been extracted. (Illustrated Dictionary of Dentistry, 1982)Methanol: A colorless, flammable liquid used in the manufacture of FORMALDEHYDE and ACETIC ACID, in chemical synthesis, antifreeze, and as a solvent. Ingestion of methanol is toxic and may cause blindness.Water: A clear, odorless, tasteless liquid that is essential for most animal and plant life and is an excellent solvent for many substances. The chemical formula is hydrogen oxide (H2O). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Dental Impression Materials: Substances used to create an impression, or negative reproduction, of the teeth and dental arches. These materials include dental plasters and cements, metallic oxide pastes, silicone base materials, or elastomeric materials.Gloves, Protective: Coverings for the hands, usually with separations for the fingers, made of various materials, for protection against infections, toxic substances, extremes of hot and cold, radiations, water immersion, etc. The gloves may be worn by patients, care givers, housewives, laboratory and industrial workers, police, etc.Protective Clothing: Clothing designed to protect the individual against possible exposure to known hazards.Eye Protective Devices: Personal devices for protection of the eyes from impact, flying objects, glare, liquids, or injurious radiation.Clothing: Fabric or other material used to cover the body.Contact Lenses: Lenses designed to be worn on the front surface of the eyeball. (UMDNS, 1999)Hockey: A game in which two parties of players provided with curved or hooked sticks seek to drive a ball or puck through opposite goals. This applies to either ice hockey or field hockey.Facial Injuries: General or unspecified injuries to the soft tissue or bony portions of the face.Gloves, Surgical: Gloves, usually rubber, worn by surgeons, examining physicians, dentists, and other health personnel for the mutual protection of personnel and patient.Eye Injuries: Damage or trauma inflicted to the eye by external means. The concept includes both surface injuries and intraocular injuries.Bromcresol Purple: An indicator and reagent. It has been used for several purposes including the determination of serum albumin concentrationsBromcresol Green: An indicator and reagent. It has been used in serum albumin determinations and as a pH indicator.Acridine Orange: A cationic cytochemical stain specific for cell nuclei, especially DNA. It is used as a supravital stain and in fluorescence cytochemistry. It may cause mutations in microorganisms.Bromphenol Blue: A dye that has been used as an industrial dye, a laboratory indicator, and a biological stain.AcridinesRosaniline Dyes: Compounds that contain the triphenylmethane aniline structure found in rosaniline. Many of them have a characteristic magenta color and are used as COLORING AGENTS.Fluorescent Dyes: Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.Staining and Labeling: The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.Azure Stains: PHENOTHIAZINES with an amino group at the 3-position that are green crystals or powder. They are used as biological stains.Coloring Agents: Chemicals and substances that impart color including soluble dyes and insoluble pigments. They are used in INKS; PAINTS; and as INDICATORS AND REAGENTS.Patents as Topic: Exclusive legal rights or privileges applied to inventions, plants, etc.Aequorin: A photoprotein isolated from the bioluminescent jellyfish Aequorea. It emits visible light by an intramolecular reaction when a trace amount of calcium ion is added. The light-emitting moiety in the bioluminescence reaction is believed to be 2-amino-3-benzyl-5-(p-hydroxyphenyl)pyrazine (AF-350).Luminescent Measurements: Techniques used for determining the values of photometric parameters of light resulting from LUMINESCENCE.Transition Elements: Elements with partially filled d orbitals. They constitute groups 3-12 of the periodic table of elements.Inventions: A novel composition, device, or process, independently conceived de novo or derived from a pre-existing model.Intellectual Property: Property, such as patents, trademarks, and copyright, that results from creative effort. The Patent and Copyright Clause (Art. 1, Sec. 8, cl. 8) of the United States Constitution provides for promoting the progress of science and useful arts by securing for limited times to authors and inventors, the exclusive right to their respective writings and discoveries. (From Black's Law Dictionary, 5th ed, p1014)Metals: Electropositive chemical elements characterized by ductility, malleability, luster, and conductance of heat and electricity. They can replace the hydrogen of an acid and form bases with hydroxyl radicals. (Grant & Hackh's Chemical Dictionary, 5th ed)Foramen Ovale, Patent: A condition in which the FORAMEN OVALE in the ATRIAL SEPTUM fails to close shortly after birth. This results in abnormal communications between the two upper chambers of the heart. An isolated patent ovale foramen without other structural heart defects is usually of no hemodynamic significance.Peptides: Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.H-Y Antigen: A sex-specific cell surface antigen produced by the sex-determining gene of the Y chromosome in mammals. It causes syngeneic grafts from males to females to be rejected and interacts with somatic elements of the embryologic undifferentiated gonad to produce testicular organogenesis.Sex Characteristics: Those characteristics that distinguish one SEX from the other. The primary sex characteristics are the OVARIES and TESTES and their related hormones. Secondary sex characteristics are those which are masculine or feminine but not directly related to reproduction.Semen: The thick, yellowish-white, viscid fluid secretion of male reproductive organs discharged upon ejaculation. In addition to reproductive organ secretions, it contains SPERMATOZOA and their nutrient plasma.Sex Chromosomes: The homologous chromosomes that are dissimilar in the heterogametic sex. There are the X CHROMOSOME, the Y CHROMOSOME, and the W, Z chromosomes (in animals in which the female is the heterogametic sex (the silkworm moth Bombyx mori, for example)). In such cases the W chromosome is the female-determining and the male is ZZ. (From King & Stansfield, A Dictionary of Genetics, 4th ed)Spermatozoa: Mature male germ cells derived from SPERMATIDS. As spermatids move toward the lumen of the SEMINIFEROUS TUBULES, they undergo extensive structural changes including the loss of cytoplasm, condensation of CHROMATIN into the SPERM HEAD, formation of the ACROSOME cap, the SPERM MIDPIECE and the SPERM TAIL that provides motility.Sperm Motility: Movement characteristics of SPERMATOZOA in a fresh specimen. It is measured as the percentage of sperms that are moving, and as the percentage of sperms with productive flagellar motion such as rapid, linear, and forward progression.Sex Factors: Maleness or femaleness as a constituent element or influence contributing to the production of a result. It may be applicable to the cause or effect of a circumstance. It is used with human or animal concepts but should be differentiated from SEX CHARACTERISTICS, anatomical or physiological manifestations of sex, and from SEX DISTRIBUTION, the number of males and females in given circumstances.Semen Preservation: The process by which semen is kept viable outside of the organism from which it was derived (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism).Probability: The study of chance processes or the relative frequency characterizing a chance process.Love: Affection; in psychiatry commonly refers to pleasure, particularly as it applies to gratifying experiences between individuals.Fixatives: Agents employed in the preparation of histologic or pathologic specimens for the purpose of maintaining the existing form and structure of all of the constituent elements. Great numbers of different agents are used; some are also decalcifying and hardening agents. They must quickly kill and coagulate living tissue.Color: The visually perceived property of objects created by absorption or reflection of specific wavelengths of light.Quantum Dots: Nanometer sized fragments of semiconductor crystalline material which emit PHOTONS. The wavelength is based on the quantum confinement size of the dot. They can be embedded in MICROBEADS for high throughput ANALYTICAL CHEMISTRY TECHNIQUES.Protein Array Analysis: Ligand-binding assays that measure protein-protein, protein-small molecule, or protein-nucleic acid interactions using a very large set of capturing molecules, i.e., those attached separately on a solid support, to measure the presence or interaction of target molecules in the sample.Immunoassay: A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance.Cadmium Compounds: Inorganic compounds that contain cadmium as an integral part of the molecule.Sensitivity and Specificity: Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)Acetic Acid: Product of the oxidation of ethanol and of the destructive distillation of wood. It is used locally, occasionally internally, as a counterirritant and also as a reagent. (Stedman, 26th ed)

The use of total protein stains as loading controls: an alternative to high-abundance single-protein controls in semi-quantitative immunoblotting. (1/4)

 (+info)

Development of an affordable dye-stained microalbuminuria screening test. (2/4)

 (+info)

Tandem scanning reflected-light microscopy of cell-substratum adhesions and stress fibres in Swiss 3T3 cells. (3/4)

This paper describes two applications of the tandem scanning reflected-light microscope (TSM) for the observation of the structure of individual cells growing in tissue culture. First, the TSM is used as an alternative to interference reflection microscopy (IRM) or total internal reflection aqueous fluorescence microscopy (TIRAF) to observe cell-substratum adhesions in unstained living cells growing on a glass coverslip. Second, the TSM is used to produce improved images of cellular structures in 3T3 cells stained with various protein dyes including Napthol Blue Black (NBB) and Coomassie Brilliant Blue (CBB). More specifically, close contacts and focal contacts are resolved in living 3T3 cells, and features of the nucleus, the cytoskeleton and extracellular matrix are resolved in both NBB- and CBB-stained cells. The focal contacts and associated stress fibres are clearly imaged in NBB-stained cells. The TSM is an improvement over conventional incident light microscopy because of the confocal image excludes information from out-of-focus regions of the cytoplasm, and, unlike the laser-based confocal microscope, the actual colour of the specimen is viewed directly with TSM in almost real-time.  (+info)

Remazol Brilliant Blue as a pre-stain for the immediate visualization of human serum proteins on polyacrylamide gel disc electrophoresis. (4/4)

We describe the pre-staining of proteins in normal human serum with Remazol Brilliant Blue before separation by disc electrophoresis. Critical to reproducibility are: dye concentration of 0.16 mol/L in a Tris-glycine buffer (pH 8.3), use of equal volumes of serum and dye solution, a tagging period of 2 h at room temperature, and electrophoresis of 0.1 mL of the mixture at 2.5 mA/gel for about 2 h. Advantages include speed, quality of resolution, and low background. This method was compared with Amido Black post-electrophoresis staining in 35 sera. Of these, 16 showed identical results with respect to the number of bands. In the other 19, Amido Black showed more bands in the post-transferrin region and, sometimes, in the post-albumin region. The pre-stained gels showed slower electrophoretic mobilities of the components. Protein bands eluted from pre-stained gels retained immunological reactivity.  (+info)

*Amido black 10B

It stains the proteins in blood a blue-black color. Amido Black can be either methanol or water based as it readily dissolves ... Amido black 10B is an amino acid staining azo dye used in biochemical research to stain for total protein on transferred ... Western blot normalization "AMIDO BLACK 10B". chemicalland21.com. Retrieved December 2, 2017. MSDS at Oxford University. ...

*Gel electrophoresis

They are visualised using Napthal Black or Amido Black staining. Typical starch gel concentrations are 5% to 10%. Denaturing ...

*Bradford protein assay

Ultraviolet-visible spectroscopy Biuret protein assay Lowry protein assay BCA protein assay Amido black protein assay Ninfa, ...

*Blotting matrix

... although they may result in the binding of anionic dyes such as Coomassie blue and Amido black. Polyvinylidene fluoride ...

*Naphthalenesulfonate

Examples include: amaranth dye amido black armstrong's acid congo red evans blue suramin trypan blue Naphthalenesulfonates at ...

*Dicopper chloride trihydroxide

... such as amido black, and indigo carmine. Copper is one of the most critically important of the trace minerals that are ... It was found in fumarolic deposits, and a weathering product of sulfides in subsea black smoker deposits. It was named for the ...

*Van Gieson's stain

Other dyes used in connection with van Gieson staining include: Alcian blue Amido black 10B Verhoeff's stain Jocelyn H. Bruce- ...

*List of MeSH codes (D02)

... amido black MeSH D02.886.645.600.080.050.650.050 --- anilino naphthalenesulfonates MeSH D02.886.645.600.080.050.650.250 --- ...

*List of MeSH codes (D04)

... amido black MeSH D04.615.638.555.282 --- anilino naphthalenesulfonates MeSH D04.615.638.555.300 --- congo red MeSH D04.615. ...

*Uranium(III) iodide

It is a black solid. Uranium triiodide can be obtained from the direct reaction of its constituent elements: 2 U + 3 I2 → 2 UI3 ... David L. Clark, Alfred P. Sattelberger (1997). "Lewis Base Adducts of Uranium Triiodide and Tris[Bis(Trimethylsilyl)Amido] ...

*James E. Atwater

Atwater, J.E., and Wheeler, R.R., Jr., Temperature Dependent Complex Permittivities of Graphitized Carbon Blacks at Microwave ... Atwater, J.E., and Akse, J.R., Cobalt - Poly(amido amine) Superparamagnetic Nanocomposites, Materials Letters 62, 3131-3134, ...

*Induline

... is a dye of blue, bluish-red or black shades. Induline consists of a mixture of several intensely colored species, so ... The indulines are prepared as mentioned above from aminoazo compounds, or by condensing oxy- and amido-quinones with phenylated ...

*Swiftlet

Its internal systematics is confusing; the plumage is usually dull, with shades of black, brown, and gray; from their outward ... including amido nitrogen, monoamine nitrogen, non-amino nitrogen, arginine, humin, histidine, lysine and cysteine), 30.6 g ... Aerodramus sawtelli, the Atiu swiftlet, and Aerodramus maximus, the black-nest swiftlet are the only known species which emit ... and the black-nest swiftlet. Instead of twigs, feathers and straw, these swiftlets make their nest only from strands of their ...

*Aviva Stadium

Celtic won the match 1-0 thanks to a goal from Amido Balde. There were talks ongoing about a return of the 'Dublin Decider' in ... "Ireland 22-24 New Zealand: Last-gasp All Blacks break Irish hearts". ESPN. Retrieved 24 November 2014. "Leinster v Northampton ...

*Arsine

On the other hand, the appearance of a black mirror deposit in the cool part of the equipment indicates the presence of ... Amido, Phosphido, and Arsenido Derivatives of aluminum and gallium. X-ray Structures of [tert-Bu2Ga(m-OH)]3 and [tert-Bu2Ga(m- ... With solid AgNO3, AsH3 reacts to produce yellow Ag4AsNO3, whereas AsH3 reacts with a solution of AgNO3 to give black Ag3As. The ...

*List of Belgian football transfers summer 2014

"Celtic striker Amido Baldé gets to show his skills for one season at the Freethiel" (in Dutch). waasland-beveren.be. 2014-09-01 ... "Young promising goalkeeper Thomas De Bie (KV Mechelen) strengthens green-black squad" (in Dutch). cerclebrugge.be. 2014-04-17 ...
P261:¯Avoid breathing dust/fume/gas/mist/vapours/spray.,P280:¯Wear protective gloves/protective clothing/eye protection/face protection.,P302 + P352:¯IF ON SKIN: Wash with plenty of soap and water.,P305 + P351 + P338:¯IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.. ...
1.FIX BLOOD IMPRESSION: Immerse in Methanol - 1 hour - or - Immerse in 5-Sulfosalicylic acid. 2.WORKING SOLUTION APPLICATION Squeeze bottle application of dye for 2 minutes. - or - Tray immersion of item into dye for 2 minutes. 3.RINSE SOLUTIONS Squeegee bottle used to apply rinse solution for 1 minute. - or - Tray immersion containing rinse, rock tray gently for 1 minute ...
Emir wrote: , , Dot blot your protein, same with the standard , at different concentrations, wash blots and apply amido black solution , (something like 0.1 g/100ml in 5% acetic acid, if I am not mistaken). The , visual comparison of the density of color allows you to estimate , concentration of the unknown protein. I guess you could develop such an , assay yourself without buying anything. No, the kit specifies that the *width* of the dot is used to determine the concentration, not the colour. If it had been colour I would have assumed amido black too. It also claims to be an absolute scale, with no standards required - the kit comes with a scaler to size the dots on and get the concentration from. The protocol is dilute, spot, fix, develop. Big surprise, theres no composition given in the on-line documentation for the dilution, fixative or developing buffers. love Anna -- I see you standing there, far out along the way, I want to touch you but, the night becomes the day. I count the words ...
article{bc5d1c6b-57f5-48f7-b597-b6f48d922d87, abstract = {In Western blotting, immunodetection of housekeeping proteins is routinely performed to detect differences in electrophoresis loading. The present work describes a much faster and simpler protein staining method, which is compatible with ordinary blocking conditions. In addition, the method can be used after immunodetection with superior linearity compared to ordinary staining methods. After immunoblotting and staining, protein bands can be further identified using peptide mass fingerprinting.}, author = {Welinder, Charlotte and Ekblad, Lars}, issn = {1535-3893}, keyword = {Western blot,loading control,Coomassie,MALDI-TOF,PVDF,GAPDH,peptide mass fingerprinting}, language = {eng}, number = {3}, pages = {1416--1419}, publisher = {The American Chemical Society}, series = {Journal of Proteome Research}, title = {Coomassie Staining as Loading Control in Western Blot Analysis}, url = {http://dx.doi.org/10.1021/pr1011476}, volume = {10}, year = ...
Reliable assessment of changes in target protein levels by western blot requires measurement of both the target and loading control proteins in the linear dynamic range. Stain-free technology is a novel method introduced by Bio-Rad to visualize and quantify proteins in gels and blots. Download this application note to learn how stain-free total protein measurement serves as a more reliable loading control than housekeeping proteins.
Loading control proteins are known to be constitutively and stably expressed at high levels in almost all tissues and cells. GAPDH, beta-tubulin and beta-actin, represent some of the popular loading control proteins.
Upon quantifying band densities in ImageJ, I compared the reproducibility of p-Smad2 linker after normalization to all possible combinations of loading controls: 25 = 32 combinations. For single loading control normalization, the effect on replicate-to-replicate reproducibility heavily depended on the choice of loading control. Normalizing to GAPDH decreased the p-Smad2 linker coefficient of variation by more than twofold (21 to 9%), but normalizing to tubulin had virtually no effect (Fig. 6C). This does not imply that GAPDH is always a good loading control or that tubulin is always a bad one; rather, it emphasizes the danger of relying on a single measured variable to estimate total protein content. As higher-order combinations of loading controls were tested as normalizers, I found that the coefficient of variation of p-Smad2 linker steadily improved toward 7 to 8%, consistent with values reported previously (10, 11). This reproducibility became less dependent on the specific combination of ...
Severn Biotech, Limited : Protein Stains - Electrophoresis Products PBS & Saline Solutions Hybridisation Reagents Biological Buffers & SDS Sodium Azide Solutions General Bio-chemical Products Fingerprinting Chemical Product Forensic Dyes & Chemicals HPLC Laboratory Solvents DNA / RNA Extraction Specialist Pure Water Products Protein Linker Conjugates Peptide Synthesis Reagents Alcohol Sprays Hand Sanitiser Products Customised Services Comet Assay Products Protein Antibody Reagents Scientific Plastics Catalogue Peptides Affinity Purification Materials Cytology Screening Antibodies Specials severn, biotech
GAPDH兔多克隆抗体(ab9385)可与小鼠, 大鼠, 牛, 人, 酿酒酵母, 非洲爪蟾样本反应并经WB实验严格验证,被30篇文献引用并得到10个独立的用户反馈。
I am currently using westerns to quantify a decrease in protein concentrations after knockdown using oligo-morpholinos. My protein of interest has a molecular weight of 36kDa. I was using GAPDH as a loading control, but because it has the same molecular weight as my protein of interest, I have to strip the membrane before looking at the control. That then leads to the problem of quantifying the decrease in concentration of my protein of interest ...
Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) has been cited in 525 publications. References for Human, Mouse, Rat, Hrs, Chk, Cow, Pig, Mk…
Anti-GAPDH antibody - Loading Control (ab9485) has been cited in 527 publications. References for Human, Mouse, Rat, Rabbit, Chk, Dog, Pig, RMk in ICC, ICC/IF…
ProSci Inc can help you find the perfect loading control antibody that can be customized to fit your project. Buy various antibodies online today from ProSci.
Anti-alpha Tubulin antibody - Loading Control (ab4074) has been cited in 135 publications. References for Human, Mouse, Rat, Shp, Hrs, Ce in EM, ICC, IHC, WB
Anti-GAPDH antibody - Loading Control (HRP) (ab9385) has been cited in 46 publications. References for Human, Mouse, Rat, Cow, Sc in WB
引用Abcams Anti-Actin抗体- Loading Control (ab1801)的参考文献列表。为您列举引用本产品的发表文章,并提供信息包括论文文献数据库中的检索编号以便您搜寻文章
Orochem offers a wide variety of total protein stains for in-gel protein detection and visualization. A wide selection of stains developed at Orochem varies in sensitivity, staining time and reversibility.
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The invention relates to the staining of poly(amino acids), including peptides, polypeptides and proteins in gels and on solid supports, using neutral or anionic complexes of transition metals.
Western blot, a method to separate proteins by size and analyse their relative expression levels, is a much maligned technique of molecular cell biology. The website PubPeer is flooded with evidence of manipulated Western blots, where gel lanes were inappropriately spliced, or where bands digitally duplicated or erased. Some even question the technology as such,…
Western blot, a method to separate proteins by size and analyse their relative expression levels, is a much maligned technique of molecular cell biology. The website PubPeer is flooded with evidence of manipulated Western blots, where gel lanes were inappropriately spliced, or where bands digitally duplicated or erased. Some even question the technology as such,…
Reagents, Tools and Custom Services for molecular biology, specializing in the fields of Nano-Antibody development (nAb), Cellular Reprogramming (iPSC), Genome Editing, Fluorescent Proteins, RNAi, Viral Packaging and Protein expression.
The two tables below display ChEMBL single-protein targets which are predicted to interact with CHEMBL25. A 1uM and 10 uM cut-off have been applied to ChEMBL bioactivity data used to generate the respective models and the green coloured rows correspond to genuine predictions, i.e. targets not included in the original training set for this compound ...
The two tables below display ChEMBL single-protein targets which are predicted to interact with CHEMBL25. A 1uM and 10 uM cut-off have been applied to ChEMBL bioactivity data used to generate the respective models and the green coloured rows correspond to genuine predictions, i.e. targets not included in the original training set for this compound ...
How to Prepare Chicken and Rice for Dogs. Homemade chicken and rice is a bland food source often recommended by veterinarians to help dogs recover from diarrhea and/or vomiting. It is an easily digestible low-fat, single-protein, and...
Ro52 is a member of the TRIM family of single-protein E3 ligases and is also a target for autoantibody production in systemic lupus erythematosus and Sjo¨ grens syndrome. We previously demonstrated a novel function of Ro52 in the ubiquitination and proteasomal degradation of IRF3 following TLR3/4 stimulation. We now present evidence that Ro52 has a similar role in regulating the stability and activity of IRF7. Endogenous immunoprecipitation of Ro52-bound proteins revealed that IRF7 associates with Ro52, an effect which increases following TLR7 and TLR9 stimulation, suggesting that Ro52 interacts with IRF7 post-pathogen recognition. Furthermore, we show that Ro52 ubiquitinates IRF7 in a dose-dependent manner, resulting in a decrease in total IRF7 expression and a subsequent decrease in IFN-a production. IRF7 stability was increased in bone marrow-derived macrophages from Ro52-deficient mice stimulated with imiquimod or CpG-B, consistent with a role for Ro52 in the negative regulation of IRF7 signalling
A blend of inorganic acids with a low foam detergent designed to rapidly attack scale and stone to leave metal surfaces sparkling and is especially formulated to passivate and re-passivate stainless steel.This acid blend will help remove the stubborn protein stain (blue rainbow) as well as food deposits like starch. ...
Video articles in JoVE about receptors erythropoietin include A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors, Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay, High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension, A High-content Imaging Workflow to Study Grb2 Signaling Complexes by Expression Cloning, Method of Direct Segmental Intra-hepatic Delivery Using a Rat Liver Hilar Clamp Model, Electrophysiology on Isolated Brainstem-spinal Cord Preparations from Newborn Rodents Allows Neural Respiratory Network Output Recording, Use of a Hanging Weight System for Coronary Artery Occlusion in Mice, Pressure Controlled Ventilation to Induce Acute Lung Injury in Mice, V3 Stain-free Workflow for a Practical, Convenient, and Reliable Total Protein Loading Control in Western Blotting, Remote Limb Ischemic Preconditioning: A Neuroprotective Technique in
Western Blot not showing protein - posted in Molecular Biology: Hi all, I have doing western blots for a while and am perplexed by some recent results. I quantified all my protein samples by Bradford and loaded them on a gel. When I blotted with tubulin (as loading control) I only saw a bands in 2 of my 5 samples. The others had no bands. I then decided to Ponceau stain my membrane and it showed equivalent loading of proteins in all 5 lanes! I then tried blotting with a second l...
Fig. 2. (A-J) Regulation and requirement of Xmxi1 during primary neurogenesis. Whole-mount in situ hybridization of stage 14 embryos injected with Sox3 (50 pg), X-ngnr-1 (50 pg), NeuroD (500 pg), Notch-ICD (50 pg), or DN-Su(H) (300 pg), as indicated in the upper right hand corner. The antisense probes used are indicated in the lower left hand corner. The injected side (β-gal, light blue) is on the right, and embryos are shown as a dorsal view, anterior down. Red arrowhead marks ectopic expression (A, C) or the increased density of the lateral stripe (I, J). (K) Xmxi1 is activated by X-ngnr-1 and SoxD, but not by the neural inducer noggin. RT-PCR analysis of animal caps isolated from embryos injected with noggin (50 pg), X-ngnr-1 (25 pg), and SoxD (200 pg), as indicated. Histone H4 was used as a loading control. CC, control caps; CE, control embryos. (L, M) N-tubulin expression of stage 14 embryos injected with X-ngnr-1-MO (15 ng) and X-ngnr-1 (25 pg), as indicated in the upper right hand ...
Coimmunoprecipitation of ATP11B with STX6 or VAMP4 in A2780-PAR and A2780-CP20 cell lysates. All coimmunoprecipitation lanes were run on the same gel but were noncontiguous. Input lanes (1:10 of original cell lysate) were run on a second gel simultaneously and detected with anti-ATP11B. Association of ATP11B with STX6 or VAMP4 was stronger in the presence of 0.5 μM cisplatin. Densitometry analysis of ATP11B protein was performed with ImageJ software. Data were normalized to a loading control (β-actin from original lysate) and are representative of 2 independent experiments ...
Figure 6. Immunoblots demonstrating localization of PSA3 to the stromal face of the thylakoid membrane. Proteins were fractionated by SDS-PAGE, transferred to nitrocellulose, and probed with the indicated antibodies. An excerpt of an image of each blot stained with Ponceau S is shown to illustrate the total population of proteins in each lane and to serve as a loading control. The large subunit of Rubisco (RbcL) is marked. A, Immunoblot analysis of total leaf proteins from maize, demonstrating specificity of the PSA3 antibody. The size of the major protein detected in the wild type (WT; ∼26 kD) corresponds to that predicted for mature Zm-PSA3. Several cross-reacting proteins accumulate to elevated levels in the mutants. The identities of these proteins are not known. B, Relative PSA3 concentration in extracts of leaf, purified mitochondria (Mito), and purified chloroplasts (CP). A blot that had been used in a previous publication (Kroeger et al., 2009) was reprobed to detect PSA3. D1 (the PsbA ...
Figure 4. Induction of the signaling pathway in the presence of interleukin 1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), and interferon gamma (IFN-γ). In ARPE-19 cells, cell lysates were analyzed with western blot for phosphorylated MAP kinase signaling protein (extracellular-signal-regulated kinases [ERK1/2], p38, and c-Jun N-terminal kinase [JNK]) and IκB and total mitogen activating protein (MAP) kinase signaling protein and IκB after IL-1β (10 ng/ml; A), TNF-α (10 ng/ml; B), or IFN-γ (10 ng/ml; C) administration. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a loading control. Immunoblot analyses of ERK1/2, p-ERK1/2, p38, p-p38, JNK, p-JNK, IκB, and p-IκB and densitometric quantification (bottom) with or without IL-1β, TNF-α, or IFN-γ, as indicated. Results are representative for three independent experiments. *p,0.05 versus vehicle ...
ProSci offers antibody beads, loading control antibodies, antibody detection sets, immunoblots, and more. Order various research antibodies from ProSci.
Figure: SETD7 is expressed at very low levels in pluripotent human cells and induced during differentiation. (A) Average rank of the top 700 most differentially expressed genes between pluripotent (iPSCs or ESCs) and fibroblasts, including those upregulated in pluripotent cells (left panel) and upregulated in fibroblasts (right panel). (B) SETD7 mRNA levels in human ESCs grown under self-renewal conditions (UndES[4]), in vitro differentiated human ESCs (DifES[4]), human fibroblasts (HFF), two lines of human keratinocytes (HEK1 and HEK2) and two lines of iPSCs generated from keratinocytes ([H]KiPS4F and KiPS4F1). Mean and standard deviation of three technical replicates is shown. Induction of SETD7 mRNA levels during ES[4] differentiation was confirmed in more than four independent differentiation experiments. (C) Western blot showing SETD7 protein levels in pluripotent and somatic cells. Loading control beta actin (ACTB) is also shown. (D) Western blot showing protein levels of SETD7, AFP, OCT4 ...
Total RNA was extracted with TRIzol® (Invitrogen, Carlsbad, CA) followed by DNAse treatment with Turbo Free DNAse® (Ambion), using the manufacturers recommended protocols. The samples underwent column purification using the RNeasy mini kit® (Qiagen, Valencia, CA). Total RNA underwent spectrophotometric analysis using a Nanodrop 1000™ and samples with A260/A280 ratio greater than 1.7 were used. Random samples underwent further analysis with an Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA) to assess RNA quality via confirmation of sharp peaks of 18S and 28S ribosomal RNA without "shoulders" or degradation. Reverse transcription of 500 ng of total RNA was carried out using First Strand Kit (SABiosciences, Frederick, MD), according to manufacturers directions. Complementary DNA was combined with a RT-PCR master mix containing SYBR® green (Life Technologies, Grand Island, NY) and ROX™ (Affymetrix, Santa Clara, CA) (loading control) and plated with primers for 84 ...
Mouse Monoclonal. Reactivity: Hu, Ms, Dog, Pig, Rat, Cat, Fsh, Rb. Application: Apuri, CC, Control, ELISA, ICC/IF, IP, Puri, WB. Specificity: This antibody can be used as a loading control antibody. GAPDH is a 146 kDa tetramer composed of four 30-40 kDa subunits. There is no cross-reaction with GAPDH from yeast.. Package: 100μg. ...
Locally designed siRNAs targeting ASIC1 (si-ASIC1a/1b, GCCAAGAAGUUCAACAAAUdtdt), ASIC2 (si-ASIC2a/2b, UGAUCAAAGAGAAGCUAUUdtdt) and ASIC2a (si-ASIC2a, AGGCCAACUUCAAACACUAdtdt) have been validated in vitro in COS-7 cells transfected with myc-ASIC1a, ASIC1b, myc-ASIC2a or myc-ASIC2b, and the relevant siRNA or a control siRNA (si-CTR, GCUCACACUACGCAGAGAUdtdt) with TransIT-LT1 and transIT-TKO (Mirus), respectively. Cells were lysed 48 h after transfection and processed for western blot analysis to assess the amount of ASIC1a protein with the anti-Myc A14 antibody (1:500; Santa Cruz Biotechnology) or the anti-ASIC1 antibody (N271/44; 1:300; NeuroMab) and a monoclonal antibody against actin (AC-40; 1:1,000; Sigma) as a loading control. siRNAs were intrathecally injected into mice (2 µg per mouse at a ratio of 1:4 (w/v) with i-Fect (Neuromics)) twice a day for 3 days. After 3 days of treatment, the paw-flick latency was measured and the residual effect of mambalgin-1 (intrathecal or intraplantar, 34 ...
Mouse Monoclonal. Reactivity: Hu, Ms, Dog, Pig, Rat, Cat, Fsh, Rb. Application: Apuri, CC, Control, ELISA, ICC/IF, IP, Puri, WB. Specificity: This antibody can be used as a loading control antibody. GAPDH is a 146 kDa tetramer composed of four 30-40 kDa subunits. There is no cross-reaction with GAPDH from yeast.. Package: 100μg. ...
TY - JOUR. T1 - Glutamine amido transferase in yeast. T2 - changes in activity during the cell cycle. AU - Duffus, J. H.. AU - Finbow, M. E.. AU - Slaughter, J. C.. PY - 1974. Y1 - 1974. M3 - Article. VL - 83. SP - 183. EP - 186. JO - Journal of General Microbiology. JF - Journal of General Microbiology. SN - 0022-1287. IS - 1. ER - ...
هدف این مطالعه جدا کردن اکتینومیست‌های نمک‌دوست و تحمل‌پذیر نمک با توانایی رنگبری از مواد رنگزای آزو است. به این منظور، اکتینومیست‌های نمک‌دوست و تحمل‌پذیر نمک جدا شده از پساب کارخانه نساجی مرینوس قم و دریافت شده از مرکز ذخایر ژنتیکی زیستی ایران، با توجه به توانایی رنگبری از ماده رنگزای آزوی Remazol Black B مورد غربالگری قرار گرفتند و سویه‌های M18 و M77 بیشترین رنگبری را نشان دادند. توانایی رنگبری از ماده رنگزای Remazol Black B توسط این دو سویه در حضور نمک سدیم‌کلراید (0تا 15%)، pH (11-5)، محدود دمایی (C 40-25) در شرایط ایستا مورد بررسی قرار گرفت. بهترین درصد رنگبری برای
What to do: Studies indicate even low levels of alcohol consumption increase prostate and many other cancers so no level of consumption is safe with it comes to cancer. While moderate alcohol consumption does appear to be mildly cardioprotective, the American Heart Association does not recommend alcohol intake because it increases the risk of "high blood pressure, obesity, stroke, accidents, suicide, and cancer". It recommends that if you drink alcohol, do so in moderation. This means an average of one to two drinks per day for men and one drink per day for women. A drink is defined as one 12 oz. beer, 4 oz. of wine, 1.5 oz. of 80-proof spirits, or 1 oz. of 100-proof spirits. Additionally, to help prevent prostate cancer, consume a healthy diet thats lower in meat and total fat and rich in a variety of fruits and vegetables (especially berries, tomatoes, and cruciferous vegetables such as cabbage and broccoli), beans and legumes, and omega-3 rich fish such as salmon. ...
O pinhão é a semente comestível da Araucaria angustifolia, sendo consumido no sul e sudeste do Brasil principalmente na sua forma in natura. A falta de diversidade em relação ao seu consumo acarreta a sobra da safra e a sua utilização como ração animal, o que diminui o valor de comercialização do produto, prejudicando os ganhos econômicos do produto. A semente do pinhão é composta de 34% de amido e um conteúdo muito baixo de proteínas e lipídios, o que a torna favorável para a obtenção de amido, aditivo amplamente utilizado pela indústria de alimentos. O objetivo deste trabalho foi caracterizar uma nova fonte de amido a partir de semente de Araucária angustifolia, através de um estudo detalhado da metodologia de extração do amido de pinhão, das características físico-químicas, funcionais e reológicas do grânulo e de sua modificação estrutural. Primeiramente foi determinado um protocolo para a extração do amido e realizado o estudo do efeito do tratamento ...
Fig. 2C -STAT 3 loading control is actually the b-acting loading control from Fig. 4B of 20103603. (3 years apart, and hes using the same image for 2 totally different things. Also, theres evidence of material being erased from the top-right corner of the image on both cases - thats how we know they are the same!) ...
Fig. 2C -STAT 3 loading control is actually the b-acting loading control from Fig. 4B of 20103603. (3 years apart, and hes using the same image for 2 totally different things. Also, theres evidence of material being erased from the top-right corner of the image on both cases - thats how we know they are the same!) ...
a) pRB was induced by tetracycline in a stably transfected SAOS2 cell line (SAOS-tetRB) and protein samples for immunoblotting were harvested at various times after pRB induction. GAPDH, loading control. Uncropped images of the gels are shown in the Supplementary Information, Fig. S6e. (b) pRB-mediated G1 arrest of corresponding samples was monitored by FACS counting BrdU-incorporating S-phase cells after tetracycline treatment of parental SAOS2 cells that do not carry inducible pRB (parental, grey bars), and of pRB-inducible SAOS2 as in a (tet-pRB). (c) pRB-APCcdh1 association was monitored over time by immunoprecipitation using anti-pRB antibodies. The pRB antibody concentration was limiting with regard to extract to obtain equal quantities of immunoprecipitated pRB at the different times. Ig: heavy chain. Uncropped images of the gels are shown in the Supplementary Information, Fig. S6f. (d, e) As controls for the specificity of APCcdh1- and pRB-mediated Skp2 protein turnover, SAOS-tetRB cells ...
Rockland Immunochemicals specializing in Akt Signaling Antibodies Antibody, Custom Assay Development, Custom Antibody Production, Loading Control Antibodies, DyLight Dye Conjugated Antibodies, Secondary Antibody Conjugates, Custom Monoclonal Antibody Production, Anti-GFP Green Fluorescent Protein, Multiplex Fluorescent Western Blotting Assay, Akt PI3 Kinase Signaling Antibodies, Western Blot Blocking Buffer, Streptavidin Peroxidase HRP ELISA, DyLight Dye Immuno fluorescence microscopy, NFkB p65 Antibody, High Content Screen Assay. Rockland Immunochemicals has supported the life science industry for over 40 years with a full range of the highest quality primary and secondary antibodies, fusion proteins, substrates, standards, and controls for basic research, assay development, and preclinical studies.
Figure 1. Cdh1 phosphorylation at Cdk sites promotes Cdh1 stability. A, Schematic of nine conserved sites of potential Cdk phosphorylation in Cdh1 (S/T-P), numbered according to human Cdh1. B, Lysates of 293T cells transfected with GFP-Cdh1 WT, 9A, or 9D were immunoblotted using a polyclonal antibody to Cdh1 or Erk, the latter to serve as a loading control. Quantitation of Cdh1 levels normalized by Erk revealed that the levels of the 9D and 9A mutant proteins were, respectively, increased by 122% and reduced by 57% relative to wild-type Cdh1 in 293T cells (average of 4 experiments). C, Lysates of Neuro2A cells transfected with GFP-Cdh1 WT, 9A, or 9D and treated with MG132 (5 μm) or the vehicle control DMSO were immunoblotted for GFP or Erk. The levels of the 9D and 9A mutant proteins were, respectively, increased by 150% and reduced by 37% relative to wild-type Cdh1 in Neuro2A cells (average of 2 experiments). MG132 treatment, respectively, increased WT, 9A, and 9D levels by 80, 134, and 9%. D, ...
Fig. 3. Glucose is required for the PM localization of K-RasG12V and LactC2 in cells overexpressing ATP5G1(1-67). (A) Cell lysates of MDCK cells stably co-expressing mGFP-K-RasG12V or mGFP-H-RasG12V with RFP alone (−) or ATP5G1(1-67)-RFP (+) were immunoblotted for phospho-ERK, phospho-Akt (S473), and mGFP-RasG12V, and quantified (mean±s.e.m.) from three independent experiments. Significant differences between cells not expressing (−) or expressing (+) ATP5G1(1-67) were assessed using Students t-test (*P,0.05; ***P,0.001). Representative blots of are shown, with total ERK, total Akt and actin used as loading controls. (B) MDCK cells stably expressing mGFP-K-RasG12V in the presence (+) or absence (−) of ATP5G1(1-67)-RFP were maintained in complete growth medium (DMEM+10% FBS+2 mM L-glutamine). When cells reached full confluence, the growth medium was replaced with fresh complete growth medium and cells were further incubated for indicated time points. Cells were fixed with 4% PFA and ...
Figure 7. siRNA-mediated depletion of 4E-T affects mRNA turnover. HeLa cells were transfected with siRNA against 4E-T or a control (Ctrl) siRNA (4AIII inverted). Protein and RNA were harvested for use in Western (C and D) and Northern (A, B, and E) analysis, respectively. (A and B) HeLa cells were transfected with siRNA against 4E-T or with control siRNA and later cotransfected with pTet-β-globin or pTet-β-globin c-fos or GM-CSF ARE and pTet Off. Cells were treated with doxycycline (1 μg/ml) ∼72 h after transfection to block transcription. Total RNA was isolated at 0.5, 2, 3, and 4 h for β-globin/GM-CSF ARE assays (A) and 0.5, 1.5, 3, and 6 h for β-globin/c-fos ARE assays (B) and analyzed by Northern blot. GAPDH served as a loading control. mRNA half-lives were calculated from Northern blots and normalized against GAPDH levels. (C) Protein extracts were collected at 6 h after treatment with doxycycline (1 μg/ml) from cells that were transfected with the reporter plasmid and pTetOff and ...
Analysis of gene expression data requires a stable reference or loading control. This reference is usually one or more reference genes. Suitable reference genes are those which are unaffected by differences in samples and experimental treatments and these must be determined for each experimental model. When performing a trial to select stable reference genes it is critical that the genes selected are from different biological pathways and that their expression is independently regulated. Ideally all reference gene candidates are tested on a selection of five test and five control samples.. ...
Fig. 4B - this is a b-actin blot. It is IDENTICAL to a STAT3 loading control used in Figure 2C of PMID 17505005 (Clin Cancer Res. 2007 May 15;13(10):3024-32.). Something is erased from the top right coner in both images, so we know theyre the same. ...
Figure and Images: Expression of functional MMP-9 by GLV-1h255-infected tumor cells. (A) Expression cassettes of GLV-1h68 and GLV-1h255. In GLV-1h255 the insert in the Tk locus was replaced by the human mmp-9 gene under control of the PSE promoter. PSEL, synthetic early/late promoter; PSE, synthetic early promoter; P7.5, VACV p7.5 K early/late promoter; P11, VACV p11 late promoter; Tk, thymidine kinase locus, Ha, hemagglutinin locus. (B) Expression of virus-encoded MMP-9 (92 kDa) in GLV-1h255 infected PC-3 cells and supernatants in vitro, β-actin (42 kDa) was used as a loading control. (C) Activity of the MMP-9 protein was tested by gelatin zymography. Lysates and supernatants of infected A549 cells were isolated and separated by non-reducing SDS-PAGE. In zymography, cleavage of the substrate by MMP-9 resulted in a clear band. ...
Neutral cobalt(II) complexes of the dianionic tetrapodal pentadentate ligand B2Pz4Py, in which borate linkers supply the anionic charges, are reported. Both the six-coordinate THF adduct 1-THF and the five-coordinate THF-free complex 1 are in a high-spin S = 3/2 configuration in the ground state and have been structurally characterized by X-ray crystallography. These two Co(II) starting materials react rapidly with aryl azides of moderate steric bulk. The thermodynamic products of these reactions are low-spin, diamagnetic, Co(III) amido complexes that are either monomeric, when an external hydrogen atom source such as 1,4-cyclohexadiene is present, or dimeric products formed via C-C coupling of the azide aryl group and internal transfer of H• to the nitrogen. These products are fully characterized and are rare examples of octahedral Co amido compounds; structural determinations reveal significant pyramidalization of the amido nitrogens due to π-π repulsion wherein the amido ligand is ...
N-[2-(Substituted amido)phenyl]-N,N-bis-carbonylguanidines bearing an optionally substituted phenoxy, phenylthio, phenylsulfinyl or phenylsulfonyl group in the 4- or 5-position of the 2-(substituted amido)phenyl group are anthelmintic agents. The compounds, of which N-(2-acetamido-4-phenylthiophenyl)-N,N-bis-carbomethoxyguanidine is a typical example, are prepared through the reaction of isothiourea-S-alkyl ether and an appropriately substituted 2-aminoanilide.
4HVG: 3-Amido Pyrrolopyrazine JAK Kinase Inhibitors: Development of a JAK3 vs JAK1 Selective Inhibitor and Evaluation in Cellular and in Vivo Models.
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EZBlue Coomassie Brilliant Blue G-250 colloidal protein stain improves protein electrophoresis results while significantly reducing staining time. Conveniently packaged, EZBlue requires no messy weigh-ups or additions of methanol or acid. As a colloidal stain, it reacts only with proteins, not the gel itself.
A quick glance past the purulent picture reveals such gems as "guitar-string needles," "computer ink-jet printer cartridge," and "park bench." Interesting also that the pustules were peripheral to the ink itself, perhaps indicative of lymphatic drainage of the tattoo site.. Taras comment thread was abuzz when she revealed herself as having not one, but two, tattoos. Speculation continues to abound as to the physical sites of her body ink, owing to her own statement, "Theyre not in oft-seen areas under normal attire, but neither are they anywhere "naughty.". This led me, of course, to wonder how exactly do tattoos work. Like anyone who watches CSI knows, the epidermis is constantly sloughing off dead skin cells as new skin rises to replaces it. This is why Coomassie Blue protein stain colors your fingers for a couple of days, until those skin cells fall off.. Being tattoo-naive, I was aware that needles are involved in the process, but I hadnt truly appreciated that the dermis underlying the ...
A range of chemical techniques were utilised for the enhancement of footwear impressions deposited on a variety of fabric types of different colours with urine as a contaminant. A semi-automated stamping device was used to deliver test impressions at a set force to minimise the variability between impressions; multiple impressions were produced and enhanced by each reagent to determine the repeatability of the enhancement. Urine samples from different donors were analysed using a spectrofluorophotometer revealing differences between individuals. Results indicated that the enhancement of footwear impressions in urine was possible using amino acid staining techniques whereas protein stains failed to achieve successful enhancement.. ...
Fig. 4 Enhanced phosphorylation of BLT1 by sequential exposure to increasing concentrations of LTB4.. (A) Intracellular [Ca2+] in HeLa cells expressing HA-BLT1/0N that were pretreated with 10 nM LTB4 or vehicle before being stimulated with 100 nM LTB4. The response to adenosine triphosphate (ATP) was included as a positive control. (B) HeLa cells expressing HA-BLT1/0N were stimulated with LTB4 as indicated. Membrane fractions were subjected to Phos-tag SDS-PAGE and immunoblotting for HA. WCLs were subjected to SDS-PAGE and immunoblotting for ERK1/2 and phosphorylated ERK1/2 (p-ERK1/2). β-Actin is an experimental and loading control. (C) RBL-2H3, CHO-K1, and Ba/F3 cells expressing HA-BLT1/0N were stimulated with LTB4 as indicated. Membrane fractions were separated by Phos-tag SDS-PAGE and immunoblotted for HA. (D) HeLa cells expressing HA-BLT1/0N were stimulated with LTB4 in the presence or absence of 100 nM CP105696 (CP) or ZK158252 (ZK). Membrane fractions were separated by Phos-tag SDS-PAGE ...
FIG 2 Production of FlaEF and their roles in motility and flagellation of V. vulnificus. (A and B) FlaE and FlaF. Cell lysates and the culture supernatants were prepared from the wild-type, ΔflaABCD, and ΔflaJ strains of V. vulnificus. Sixty micrograms of crude cell extracts (A) and 2 μg of the concentrated proteins in culture supernatants (B) were subjected to SDS-PAGE and then Western blot analysis using polyclonal antibodies reacting with all the flagellin-like proteins from FlaA to FlaF. As loading controls for cell lysate and culture supernatant, the antibodies specific to IIAGlc and OmpU were utilized, respectively. The ΔflaJ strain was included as a negative control in this assay. Each immunoreactive band detected by specific antibodies is indicated with an arrow. Numbers on the left sides of the blots are molecular weight markers, in kilodaltons. (C) Motility on a soft agar plate. Fresh cultures of ΔflaABCD, flaE::nptI, ΔflaF, and ΔflaEF strains were spotted onto a soft agar ...
FIG. 7. Reversibility of Ni-induced gene expression. Expression of target genes in strain CC125 at 3 × 106 cells/ml is shown in lane 1 (t = 0). The culture was treated with either 25 μM NiCl2 for 5 h (lane 2) or 50 μM EDTA for 5 or 19 h (lanes 7 and 8) The reversibility of the 5 h Ni2+ treatment was ascertained either by addition of excess EDTA (50 μM) to chelate the Ni2+ ions or by transfer to fresh medium and then sampled 5 or 19 h later to assess gene expression (lanes 5 and 6 and lanes 9 and 10, respectively). Ni-treated cultures were maintained in parallel for another 5 or 19 h as a control for the EDTA treatment (lanes 3 and 4, respectively), or in the case of the washed cells, NiCl2 was added back, and the culture sampled 5 or 19 h later (lanes 11 and 12). The total time of exposure to Ni2+ was, therefore, 10 or 24 h. Total RNA was isolated and analyzed by RNA blot hybridization for Cyc6 and Cpx1 expression. Cβlp expression was used as a loading control. The circular arrow indicates ...
Figure 7: ATM activation in HCT 116 wt cells treated with genistein (100 μM), camptothecin CPT (1 μM), and genistein derivatives substituted at C4′ at the concentration equivalent to 3x IC50 (Ram-2′ (3 μM), Ram-3′ (40 μM), Ram-5′ (20 μM), Gen-5′ (20 μM). HSC 70 served as a loading control ...
Fig. 1. HRC is upregulated in fibrotic liver. (A) Representative immunohistochemical staining shows the expression of HRC in human samples. S0 represents no inflammation and no fibrosis of liver, S1 represents inflammation in portal area and peri-sinusoid fibrosis, S2 represents mild inflammation and fibrous septum formation. Scale bars: 100 µm. (B) Representative H&E, Masson and Sirius Red staining (100×) of TAA-induced liver fibrosis in rats. W, weeks. 4W represents rats treated with TAA for 4 weeks, 8W represents rats treated with TAA for 8 weeks. (C) Box plot (25th percentile, mean, 75th percentile) of the mRNA levels of α-SMA and HRC in TAA-induced liver fibrosis in rats measured by RT-qPCR (n=6). *P,0.05, **P,0.01. (D) Representative image shows the protein levels of α-SMA and HRC in TAA-induced liver fibrosis in rats. GAPDH was used as a loading control. ...
Fig. 2A,B,C and 4B - same b-actin loading control used for dose response and time course experiments. First 10 lanes of b-actin in 4B are the same as all 10 lanes in 2B. ...
Squaric Acid Ester Amido Polyethylene Glycols are a novel class of reactive and chemoselective PEGylation reagents. The squaric acid PEG´s are chemoselective towards the amino groups of Lysin in protein PEGylation whereas the guanidine function of Arginine, the hydroxyl group of Serine and the imidazole function of Histidine are not affected. Literature: L 59 ...
Complex (I) was synthesized in the course of our studies on amido complexes of zirconium (Kempe, 2000). The compound was obtained in the reaction Zr(NEt)4 with iPr2C6H3NH2 (molar ratio 1:2). Complex (I) contains four amido ligands (two NEt2 and two iPr2C6H3NH) and one amino ligand (HNEt2). Analyzing bond lengths in title compound it is easily spotted that bond Zr-N3 is much longer than other bonds. It is caused by the fact, that ligand containing N1 is amine ligand. Difference between length of bonds Zr-N between NEt2 and HNEt2 is about 0.25 Å. Distances between N atoms of iPr2C6H3NH and Zr are both about 2.12 Å and very similar to related Zr(IV) amido complexes (Profilet et al., 1990; Blake et al., 1997). In case of two NEt2 ligands distances Zr-N differ by about 0.12 Å but both are in the range typical for zirconium complexes with diethylamido ligands (Porter et al., 2004; Ghesner et al., 2006). Comparing angles between N1-Zr1-N2, N2-Zr1-N5 and N5-Zr1-N1 [table 1] it can be seen that they ...
4HVG: 3-Amido Pyrrolopyrazine JAK Kinase Inhibitors: Development of a JAK3 vs JAK1 Selective Inhibitor and Evaluation in Cellular and in Vivo Models.
Dear Histonetters, I am looking for stains that will identify the basic contents of cultured, mammary alveoli (mammospheres) of Kangaroo. Broad lipid ( ANY FAT STAIN THAT CAN BE DONE ON PARAFFIN EMBEDDED TISSUE???POSSIBLE??), carbohydrate (PAS?? ANY OTHER SUGGESTIONS) and protein stains (???) are required to determine what - IF any, different hormone combinations have/ effect on milk production. Any suggestions would be wonderful......our Histologist has made several good suggestions for me to try & we are both interested to see WHAT POSSIBILITIES/suggestions the scientific minds in Histo-land come up with. Thankyou in advance & Cheers, Sonia -- BRUCE ABALOZ PH:61383446282 HISTOLOGIST FAX:61383447909 DEPT.of ZOOLOGY EMAIL: [email protected] THE UNIVERSITY Of MELBOURNE. VICTORIA. AUSTRALIA 3010 Nobody Can Make You Feel Inferior Without YOUR Permission - Eleanor Roosevelt DANCE LIKE NO-ONES WATCHING Q: Whats a specimen? A: An Italian astronaut. ...
In this study, we have used different proteomic techniques to compare and characterize multiple proteins simultaneously in Ig-treated monocytes. We identified a number of proteins that are differentially regulated by APS IgG compared with HC IgG.. Classical proteomic techniques measure the intensity of the 2D PAGE protein stain to determine individual protein quantity and confirm its identity by MS. Of the additional techniques we used, 2D DiGE overcomes limitations in traditional 2D electrophoresis due to intergel variation by allowing measurement of the abundance of a protein in each sample by direct comparison with other samples as well as an internal standard (in this case a mix of IgG from all 3 groups) on each gel.. Not all proteins, however, are amenable to 2D DiGE particularly hydrophobic membrane proteins. Therefore, to ensure that we had fully characterized the proteome, we studied tryptic digests of IgG-treated monocyte proteins using LC-MS/MS label-free quantitation on a Q-TOF mass ...
Squaric Acid Ester Amido Polyethylene Glycols are a novel class of reactive and chemoselective PEGylation reagents. The squaric acid PEG´s are chemoselective towards the amino groups of Lysin in protein PEGylation whereas the guanidine function of Arginine, the hydroxyl group of Serine and the imidazole function of Histidine are not affected. Literature: L 59 ...
The Cas9 expression vector, containing an N-terminal MBP tag and C-terminal mCherry, was kindly provided by Dr. Jennifer Doudna. The protein was expressed in BL21 Rosetta cells for three hours at 18 °C. Cells were pelleted and frozen. Upon thawing, cells from a 4 L culture preparation were resuspended in 50 mL of lysis buffer (50 mM sodium phosphate pH 6.5, 350 mM NaCl, 1 mM TCEP (tris(2-carboxyethyl)phosphine), 10 % glycerol) supplemented with 0.5 mM EDTA, 1 μM PMSF (phenylmethanesulfonyl), and a single Roche complete EDTA-free protease inhibitor tablet (Roche Diagnostics, Indianapolis, IN, USA) and passed through an HC-8000 homogenizer (Microfluidics, Westwood, MA, USA) five times. The lysate was clarified by centrifugation at 20,000 rpm for 45 min at 4 °C and then filtered through a 0.22 μm vacuum filtration unit. The filtered lysate was loaded onto three 5 mL HiTrap Heparin HP columns (GE Healthcare, Little Chalfont, UK) arranged in series on a GE AKTA Pure system. The columns were ...
For critical research, you need reliable protein detection, and Life Technologies provides a complete suite of labeling tools for any level of target abundance. Our fluorescent products portfolio is the most peer-referenced in life science research. Check out this simple guide and see which tool is right for your protein. • High-abundance target-primary conjugate, no amplification needed • Medium- to high-abundance target-secondary conjugate, modest amplification • Medium- to low-abundance target-streptavidin conjugate, elevate the signal • Low-abundance target-enzyme amplification, complex process for maximum signal enhancement
CD1d-restricted natural killer T cells (NKT cells) possess a wide range of effector and regulatory activities that are related to their ability to secrete both T helper 1 (Th1) cell- and Th2 cell-type cytokines. We analyzed presentation of NKT cell activating α galactosylceramide (αGalCer) analogs that give predominantly Th2 cell-type cytokine responses to determine how ligand structure controls the outcome of NKT cell activation. Using a monoclonal antibody specific for αGalCer-CD1d complexes to visualize and quantitate glycolipid presentation, we found that Th2 cell-type cytokine-biasing ligands were characterized by rapid and direct loading of cell-surface CD1d proteins. Complexes formed by association of these Th2 cell-type cytokine-biasing αGalCer analogs with CD1d showed a distinctive exclusion from ganglioside-enriched, detergent-resistant plasma membrane microdomains of antigen-presenting cells. These findings help to explain how subtle alterations in glycolipid ligand structure can ...
8-Chloroadenosine 3′:5′-monophosphate (8ClcAMP) inhibits the growth of human glioma cell lines at much lower concentrations than more commonly used cyclic AMP analogues, without inducing morphological differentiation. The mechanism by which 8ClcAMP exerts this effect is not fully understood. We examined whether the growth-inhibitory effect of this compound is due to an active metabolite, using a sulforhodamine protein stain assay to determine the proliferation rate of the WF human glioma cell line. 8-Chloroadenosine, one of the metabolites, inhibited the proliferation of WF human glioma cells more potently than 8ClcAMP. In the presence of adenosine deaminase, which converts 8-chloroadenosine into 8-chloroinosine, 8-chloroadenosine no longer inhibited human glioma cell growth. Addition of adenosine deaminase also largely reduced the growth-inhibitory effect of 8ClcAMP, but not of 8-(4-chlorophenylthio)cAMP. High performance liquid chromatography analysis revealed that at least part of the ...
FIG. 1. The SAHH gene is a Myc target gene. (A) P493-6 cells were incubated with doxycycline (Dox) to inhibit exogenous Myc expression or vehicle control (veh). Rat1A fibroblasts, primary MEFs, and IMECs were infected with retroviruses to express vector control, MycWT, or MycΔMBII, and pools of cells were drug selected. Primary murine T cells were incubated with IL-2, IL-7, and IL-15 for 24 h. Rat1A cells were incubated with a control siRNA (con si; cyclophilin B siRNA) or an siRNA directed against Myc (Myc si) for 24 h. Western blotting was performed to detect SAHH, Myc, β-tubulin, or POLR2A expression, as loading controls. (B) RNA was extracted from the cell lines indicated, and RT-PCR was performed to detect SAHH mRNA levels relative to GAPDH (or 18S rRNA for P493-6). Prim., primary. (C) In the IMEC lines indicated, SAHH mRNA was detected by RT-PCR (black bars) and m7G (cap-methylated) SAHH mRNA was detected by anti-m7G IP followed by RT-PCR (gray bars). (D) Rat fibroblasts expressing the ...
Figure 4: Plasmin activates Akt, MAPK, and NF-κB signaling in dendritic cells. (a) Time-dependent phosphorylation of Akt (Ser473) and MAP kinases in dendritic cells after stimulation with plasmin (0.143 CTA U/mL). Dendritic cells were stimulated with plasmin, and whole cell lysates were analyzed by western immunoblotting; actin served as loading control. Representative data of three experiments are shown. (b) Time-dependent phosphorylation of IκBα (Ser32/Ser36) in dendritic cells after stimulation with plasmin (0.143 CTA U/mL). Representative data of three experiments are shown. (c) Activation of p65 NF-κB as analyzed with the NF-κB TransAM ELISA. Nuclear extracts were obtained from dendritic cells stimulated with plasmin (0.143 CTA U/mL) or LPS (0.5 μg/mL) for 1 h. The results are mean ± SEM of 3 independent experiments, *P , 0.05, **P , 0.01 versus control ...
Background The dynamic regulation of cell-cell adhesions is crucial for developmental processes, including tissue formation, differentiation and motility. Adherens junctions are important components...
Freshwater is an increasingly scarce resource that is extensively used in textile wet‐processing. In seeking to identify alternative low freshwater‐usage coloration technology, this study examined the potential use of seawater (SEAW) as the dyeing medium for wool coloration using a range of reactive dyes. Initially, the dyeing behaviour of the wool fabric in simulated seawater (SSW) was compared with conventional dyeing from distilled water (DW) using α‐bromoacrylamide‐based Lanasol dyes and sulphatoethyl sulphone‐based Remazol dyes. These preliminary studies demonstrated that comparable coloration could be achieved in the SSW medium based on an assessment of the dye exhaustion, dye fixation, colour yield and levelness. Subsequent dyeing studies of wool using Mauritian seawater with both the Lanasol and Remazol reactive dyes confirmed that, based on the dye exhaustion, dye fixation, colour yield and levelness, comparable coloration could be achieved, highlighting the possibility of ...
Methods: Mitochondrial morphology and protein expression were investigated in post-keratoplasty FECD specimens and immortalized normal and FECD human corneal endothelial cell lines (HCECi and FECDi, respectively) with and without menadione (MN), an oxidant specifically relevant for study of FECD. HCECi cells were exposed to MN (0, 25, 50, 100 µM for 2 hours) and mitochondrial morphology was assessed by cytochrome c staining using confocal microscopy. Mitochondrial fractions were obtained by suspending the fresh cells in mitochondrial isolation buffer and passing them 15 times through 25G needle. Samples were centrifuged at 500 rpm, 5min, at 4℃ and 10,000 rpm, 30min, at 4℃. The protein levels of Parkin of whole cell lysates and mitochondrial and cytosolic fractions were compared by immunoblotting. VDAC-1 was used as a loading control of mitochondrial fractions.. ...
Constrained geometry catalysts (CGC) are known to be active in the polymerization and copolymerization of alkenes with a distinct control over polymer tacticity. The tethering of one ¿5-cyclopentadienyl moiety and one pendant donor gives these compounds an accessible metal center as well as ability to maintain their structure throughout the catalytic process. Complexes of this type typically feature one pendant amido donor. Replacement of the pendant amido donor with a nitrogen heterocycle such as an indolyl- or pyrrolyl-group should result in electrophilic metal centers due to reduced N ¿ M p donation, a consequence of electron delocalization of the nitrogen lone pair in the aromatic system. This dissertation reports the development of a new series of constrained geometry ligands that feature indolyl- and pyrrolyl- donor moieties. In chapter 2, the synthesis and characterization of a series of acetal precursors and their corresponding di(3-methylindolyl)ethane and dipyrrolylethane constrained ...
Abstract Biosorption capacities and rates of different kinds of dried yeasts (Saccharomyces cerevisiae, Schizosaccharomyces pombe, Kluyveromyces marxianus, Candida sp., C. tropical..
The purity of other key intermediate i.e. 4-[2-(3-Ethyl-4-methyl-2-carbonyl pyrrolidine amido) ethyl] benzene sulfonamide (IV) is also not well documented in the literature. US 4,379,785 (EP 031058) reports condensation of crude 4-[2-(3-Ethyl-4-methyl-2-carbonyl pyrrolidine amido) ethyl] benzene sulfonamide (IV) with trans-4-methyl cyclohexyl isocyanate (VIII) to obtain Glimeperide (I). However, using this crude sulfonamide there is always a possibility of getting undesired ortho and meta isomers in Glimepiride. The present invention relates to a purification process of 4-[2-(3-Ethyl-4-methyl-2-carbonyl pyrrolidine amido) ethyl] benzene sulfonamide of Formula (IV) with a mixture of hydrocarbon and alcohol. The hydrocarbon can be aliphatic, alicyclic or aromatic .The hydrocarbon is further selected from hexane, heptane ,cyclohexane, toluene or mixtures thereof preferably, toluene. The alcohol used for crystallization is an aliphatic one,wherein,the carbon chain may be preferably CI to C4. ...
This article discusses small molecule elimination from group IVB (Ti, Zr, Hf) amido complexes. An ab initio quantum chemical analysis of HX (X = H, CH₃, Cl, NH₂, SiH₃) elimination by group IVB (Ti, Zr, Hf) amidos (H₂(X)M - NH₂ → H₂M = NH + HX), of interest in the context of CVD precursor design, is reported ...
Description: This article discusses small molecule elimination from group IVB (Ti, Zr, Hf) amido complexes. An ab initio quantum chemical analysis of HX (X = H, CH₃, Cl, NH₂, SiH₃) elimination by group IVB (Ti, Zr, Hf) amidos (H₂(X)M - NH₂ → H₂M = NH + HX), of interest in the context of CVD precursor design, is reported. ...
Lanzhou Greenchem ILs (OR Center for Greenchemistry and Catalysis) LICP, CAS. Provide you with Ionic Liquids and related services. According to cation, common ionic liquids including: Imidazolium, Pyrilidinium, Ammonium, Phosphonium, Pyrrolidinium, Piperidinium. Functionized ionic liquids including: hydroxyl, carboxyl, ether, ester, amido, sulfoacide, vinyl, benzyl, nitrile and guanidine.
TY - JOUR. T1 - Patterns of early recurrence after sentinel lymph node biopsy for melanoma. AU - Chao, Celia. AU - Wong, Sandra L.. AU - Ross, Merrick I.. AU - Reintgen, Douglas S.. AU - Noyes, R. Dirk. AU - Cerrito, Patricia B.. AU - Edwards, Michael J.. AU - McMasters, Kelly M.. AU - Clifford, Edward. AU - Biffl, Walter. PY - 2002/12/1. Y1 - 2002/12/1. N2 - Background: Patterns of early recurrence after sentinel lymph node (SLN) biopsy for melanoma was determined from the Sunbelt Melanoma Trial, which includes patients with Breslow thickness ≥1.0 mm and nonpalpable regional lymph nodes. Methods: SLN were evaluated by routine histology and S-100 protein stain. Overall, there were 1,183 patients with a median follow-up of 16 months. Results: SLN were positive in 233 of 1,183 patients (20%). The recurrence rate was greater among patients with histologically positive SLN than those with negative SLN (15.5% versus 6.0%, respectively, P ,0.05). Patients with positive SLN were more likely to have ...
Cancer-associated blood biomarkers exist in exceedingly low concentrations within complex mixtures of high-abundance proteins such as albumin and immunoglobulins. Moreover, biomarkers in the blood may be subjected to degradation during transportation and storage. Such degradation is a significant source of bias for cancer biomarker measurement and discovery. We have created N-isopropylacrylamide porous sieving core shell smart nanoparticles containing an internal affinity bait to perform three independent functions within minutes, in one step, in solution (serum or plasma): a) molecular size sieving with complete separation from high abundance residence proteins such as albumin and immunoglobulin, b) affinity capture of all solution phase target molecules, and c) complete protection of harvested proteins from enzymatic degradation. The captured analytes can be readily electroeluted for analysis. In our preliminary studies we have manufactured large quantities of uniform porous nanoparticles ...
Brilliant Blue R, pure, ACROS Organics™ 500g; Glass bottle Brilliant Blue R, pure, ACROS Organics™ Blue Stains and Dyes and Indicators
Aqueous solutions containing alkylamido betaines and certain water-soluble inorganic or organic salts provide compositions of remarkably high viscosities. The inorganic salts suitable for this purpose are the sulfates of metals from Groups IA, IIA, IIB, and IIIA of the period table; sulfates of non-metallic ions; and alkali metal carbonates. Organic salts suitable in the present compositions include the citrates, tartrates, succinates, and carboxymethyloxysuccinates of metals from Groups IA, IIA, IIB and IIIA of the period table and the citrates, tartrates, succinates and carboxymethyloxysuccinates of ammonium ions. These salts have a synergistic viscosity-building effect on aqueous compositions containing alkylamido betaines in combination with micelle-forming anionic surfactants. Other salts increase the viscosity of aqueous solutions containing alkylamido betaines only in the presence of anionic surfactants. Suitable inorganic salts in this category include sulfates of metals from Group IB of the

Amido black 10B - WikipediaAmido black 10B - Wikipedia

It stains the proteins in blood a blue-black color. Amido Black can be either methanol or water based as it readily dissolves ... Amido black 10B is an amino acid staining azo dye used in biochemical research to stain for total protein on transferred ... Western blot normalization "AMIDO BLACK 10B". chemicalland21.com. Retrieved December 2, 2017. MSDS at Oxford University. ...
more infohttps://en.wikipedia.org/wiki/Amido_black_10B

Amido black 10B | AbcamAmido black 10B | Abcam

Join researchers using high quality Naphthol Blue Black from Abcam and… ... Buy Naphthol Blue Black (CAS 1064-48-8), a water soluble histological and protein dye. ... Decolorization of naphthol blue black using the horseradish peroxidase.. Appl Biochem Biotechnol 163:433-43 (2011). Read more ( ...
more infohttps://www.abcam.com/amido-black-10b-ab146311.html

Amido Black 10B Safety Overview | National DiagnosticsAmido Black 10B Safety Overview | National Diagnostics

CAUTION! MAY BE HARMFUL IF SWALLOWED OR INHALED. MAY CAUSE IRRITATION SKIN, EYES, AND RESPIRATORY TRACT. THE TOXICOLOGICAL PROPERTIES OF THIS SUBSTANCE HAVE NOT BEEN FULLY INVESTIGATED ...
more infohttps://www.nationaldiagnostics.com/histology/msds/amido-black-10b-safety-overview

Amido Black Water-Based Kit, 32 oz. - The Safariland GroupAmido Black Water-Based Kit, 32 oz. - The Safariland Group

Amido Black reacts with blood proteins to create a deep blue color change. This formulation includes a blood fixative in the ... Amido Black is an effective blood reagent when latent or difficult to see blood stains are present. ... Amido Black Water-Based Kit, 32 oz.. MODEL: SKU: PART: Amido Black is an effective blood reagent when latent or difficult to ... Amido Black is an effective blood reagent when latent or difficult to see blood stains are present. Amido Black reacts with ...
more infohttps://www.safariland.com/products/forensics/latent-print-processing/latent-print-reagents-and-chemicals/protein-stains/amido-black-water-based-kit-32-oz.-1163036.html

Amido Black Water Base - Latent print processing reagent.Amido Black Water Base - Latent print processing reagent.

Naphthol Blue Black. Naphthalene 12B. Acid Black 1, 10A, 10B. Acid Black 12B, 10BA. Eriosin Blue Black B. Acidal Black 10B. ... Amido Black ***. ,. Forensic Light. ,. Physical Developer. AMIDO BLACK - Water Base. FORMULA:. 1st SOLUTION : (Citric Acid ... AMIDO BLACK - Water Base. Surface(s) Used On:. Blood stained non-porous surfaces, especially plastics.. Sensitive To:. Blood ... AMIDO BLACK - Water Base. [ Return to: Main Page ,, Non-Porous Surfaces ,, Glass Surfaces ,, Plastic Bag Surfaces ,, Blood ...
more infohttp://www.cbdiai.org/Reagents/amidow.html

Lightning Powder 1-2740, 1-2741 Aqueous Amido Black FREE SHIPPING!Lightning Powder 1-2740, 1-2741 Aqueous Amido Black FREE SHIPPING!

... in Methanol as in the procedure for the mixed solution of the Amido Black powder above.Shelf-life of Aqueous Amido Black: one ... 1-2741 Aqueous Amido Black Aqueous Amido Black is a pre-mixed Amido Black solution. It can be used to enhance or develop blood- ... 1-2740, 1-2741 Aqueous Amido Black. Aqueous Amido Black is a pre-mixed Amido Black solution. It can be used to enhance or ... 1-2741 Aqueous Amido Black. Lightning Powder 1-2740, 1-2741 Aqueous Amido Black. Option:. Choose Your Option 1 Liter. 1 Gallon ...
more infohttps://www.copsplus.com/lightning-powder-aqueous-amido-black

1064-48-8 - Amido Black 10B, Electrophoresis Reagent - C.I. 20470 - Naphthalene Black 10B - J66798 - Alfa Aesar1064-48-8 - Amido Black 10B, Electrophoresis Reagent - C.I. 20470 - Naphthalene Black 10B - J66798 - Alfa Aesar

J66798 Amido Black 10B, Electrophoresis Reagent Amido Black 10B can be used to stain proteins on polyacrylamide gels, agarose ... The gel can then be stained with 0.1% Amido Black 10B in a 7% (v/v) solution of acetic acid for at least 2 hours. Then de-stain ... The detection sensitivity of Amido Black 10B is approximately 20% that of Coomassie Blue R. ...
more infohttps://www.alfa.com/en/catalog/J66798/

Amido Black 10B (C.I. 20470) | PhionAmido Black 10B (C.I. 20470) | Phion

P261:¯Avoid breathing dust/fume/gas/mist/vapours/spray.,P280:¯Wear protective gloves/protective clothing/eye protection/face protection.,P302 + P352:¯IF ON SKIN: Wash with plenty of soap and water.,P305 + P351 + P338:¯IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.. ...
more infohttp://phionchemicals.com/product/amido-black-10b-c-i-20470

AMIDO BLACK 10B (For Molecular Biology) Manufacturer,Supplier,ExporterAMIDO BLACK 10B (For Molecular Biology) Manufacturer,Supplier,Exporter

... supplier and exporter of AMIDO BLACK 10B (For Molecular Biology) at reasonable price. ... AMIDO BLACK 10B (For Molecular Biology) AMIDO BLACK 10B (For Molecular Biology) ...
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Kinetics and mechanism of oxidation of amido black by sodium  N-halo-p-toluenesulfonamides in acidic medium: Spectrophotometric...Kinetics and mechanism of oxidation of amido black by sodium N-halo-p-toluenesulfonamides in acidic medium: Spectrophotometric...

Kinetics and mechanism of oxidation of amido black by sodium N-halo-p-toluenesulfonamides in acidic medium: Spectrophotometric ... Shubha, J., Vinutha, K., & Puttaswamy, P. (2013). Kinetics and mechanism of oxidation of amido black by sodium N-halo-p- ... Shubha, J.; Vinutha, K.; Puttaswamy, P. Kinetics and mechanism of oxidation of amido black by sodium N-halo-p- ... "Kinetics and mechanism of oxidation of amido black by sodium N-halo-p-toluenesulfonamides in acidic medium: Spectrophotometric ...
more infohttp://eurjchem.com/index.php/eurjchem/article/view/806/PDF

Indicators and Dyes for Chemical Processing - Alfa AesarIndicators and Dyes for Chemical Processing - Alfa Aesar

View our list of indicators and dyes used to determine pH or presence of metal ions. Purchase indicators and dyes at Alfa Aesar online.
more infohttps://www.alfa.com/en/indicators-and-dyes/

Products in Stains & Dyes, bioWORLD on Thomas ScientificProducts in Stains & Dyes, bioWORLD on Thomas Scientific

Amido black bioWORLD. Stain for detection and location of proteins. Protocol: Wash blot in 0.#% Tween/PBS, with three changes ...
more infohttps://www.thomassci.com/nav/cat1/stainsdyes/manufacturer/bioworld/0

Frontiers | A Robust and Universal Metaproteomics Workflow for Research Studies and Routine Diagnostics Within 24 h Using...Frontiers | A Robust and Universal Metaproteomics Workflow for Research Studies and Routine Diagnostics Within 24 h Using...

Protein Quantification Using Amido Black Assay (X1). For quantification of protein concentration (Supplementary Data Sheet S1) ... 50 μL of the sample were precipitated with 300 μL amido black staining solution. Afterward, the sample was centrifuged (5 min, ... The samples BGP, human gut, soil, and compost, and WWTP as well as their averages (black line) are shown separately. For ...
more infohttps://www.frontiersin.org/articles/10.3389/fmicb.2019.01883/full

Patent US6316267 - Luminescent protein stains an their method of use - Google PatentsPatent US6316267 - Luminescent protein stains an their method of use - Google Patents

Known protein stains, such as Amido Black and CBB staining, are difficult to destain, prevent subsequent immunostaining, and ... Proteins appear as white bands on a gray to black background or as black bands on light gray to white background on the ... Proteins appear as white bands on a gray to black background or as black bands on light gray to white background on the ... The stained proteins in the gel are photographed using Polaroid 667 black and white print film using a Kodak #Wratten 9 gelatin ...
more infohttp://www.google.com/patents/US6316267?dq=5179747

48 Hours: NCIS: Body of Evidence - CBS News"48 Hours: NCIS": Body of Evidence - CBS News

... they did an Amido Black test. So Amido Black reacts to protein. Proteins found in blood. So as they sprayed the stuff on the ... And that pool is exactly where Annie laid, and she bled out," Agent Jim Grebas explained of the Amido Black test. NCIS Agent ...
more infohttps://www.cbsnews.com/news/48-hours-ncis-annie-tahan-disappearance-murder-body-of-evidence/

Preferred Spellings and Usage - DiabetologiaPreferred Spellings and Usage - Diabetologia

amido black. Amido Black. (amino acid chains). Gly-Lys-Ala. amphetamine (USAN). amfetamine (INN). ...
more infohttp://diabetologia-journal.org/about-the-journal/diabetologia-style-guide/preferred-spellings-and-usage/

Molecular Vision: Technical Brief: A novel strategy for
enrichment of trabecular meshwork protease proteomeMolecular Vision: Technical Brief: A novel strategy for enrichment of trabecular meshwork protease proteome

... and Amido Black). For this purpose, we have performed experiments with porcine TM tissue to reduce or limit the use of precious ...
more infohttp://www.molvis.org/molvis/v14/a104/

Haz-Map Category DetailsHaz-Map Category Details

May cause skin and respiratory sensitization; [Sigma-Aldrich MSDS] See "Amido Black." ... May cause skin and respiratory sensitization; [Sigma-Aldrich MSDS] See "Amido Black." ...
more infohttps://hazmap.nlm.nih.gov/category-details?id=21651&table=copytblagents

Preval Disposable Sprayer Kit - The Safariland GroupPreval Disposable Sprayer Kit - The Safariland Group

Amido black is an effective blood reagent when latent or difficult to see blood stains are present. Amido black reacts with ... Amido black is an effective blood reagent when latent or difficult to see blood stains are present. Amido black reacts with ... Amido Black is an effective blood reagent when latent or difficult to see blood stains are present. Amido Black reacts with ... Amido black is an effective blood reagent when latent or difficult to see blood stains are present. Amido black reacts with ...
more infohttp://www.safariland.com/products/forensics/latent-print-processing/latent-print-reagents-and-chemicals/chemical-sprayers/preval-disposable-sprayer-kit-1004109.html

Plus itPlus it

Black DL. (2014) The splicing regulator PTBP2 controls a program of embryonic splicing required for neuronal maturation. eLife ... Amidoblack staining shown beneath immunosignals; positions of relevant size marker bands are indicated. D, Coamplification of ... White, cds; hatched, 3′ UTR in SR30.2; gray, cds in SR30.1 and 3′ UTR in SR30.2; black, HA-tag. Arrowheads indicate binding ... Gray and black fills indicate coding sequence and 3′ UTR, respectively; AS event identifiers from previous study (Hartmann et ...
more infohttp://www.plantphysiol.org/content/176/4/2886

Gel electrophoresis - WikipediaGel electrophoresis - Wikipedia

They are visualised using Napthal Black or Amido Black staining. Typical starch gel concentrations are 5% to 10%. Denaturing ...
more infohttps://en.wikipedia.org/wiki/Gel_electrophoresis

Patent US5439362 - Sex-associated membrane proteins and methods for increasing the probability ... - Google PatentsPatent US5439362 - Sex-associated membrane proteins and methods for increasing the probability ... - Google Patents

Following the transfer to NC, we stained the bands with 0.5% amido black in 7% acetic acid. (The technique of using amido black ... Louis, Mo.) We prefer to use amido black stain because it does not interfere with the subsequent preparation of anti-sera or ... We then cut out the amido black stained bands on the NC sheets that correspond to SAM proteins using a razor blade. ... with the molecular weight standards on the amido black stained transblotted SDS/PAGE 1D profiles. The alignment of the ...
more infohttp://www.google.com/patents/US5439362?dq=7,446,777
  • Amido black 10B is an amino acid staining azo dye used in biochemical research to stain for total protein on transferred membrane blots, such as the western blot. (wikipedia.org)
  • Amido Black 10B can be used to stain proteins on polyacrylamide gels, agarose gels and nitrocellulose membranes. (alfa.com)
  • The detection sensitivity of Amido Black 10B is approximately 20% that of Coomassie Blue R. (alfa.com)