Amide Synthases: Enzymes that catalyze the joining of either ammonia or an amide with another molecule, in which the linkage is in the form of a carbon-nitrogen bond. EC 6.3.1.Amides: Organic compounds containing the -CO-NH2 radical. Amides are derived from acids by replacement of -OH by -NH2 or from ammonia by the replacement of H by an acyl group. (From Grant & Hackh's Chemical Dictionary, 5th ed)Polyketide Synthases: Large enzyme complexes composed of a number of component enzymes that are found in STREPTOMYCES which biosynthesize MACROLIDES and other polyketides.Alkyl and Aryl Transferases: A somewhat heterogeneous class of enzymes that catalyze the transfer of alkyl or related groups (excluding methyl groups). EC 2.5.Chitin Synthase: An enzyme that converts UDP glucosamine into chitin and UDP. EC 2.4.1.16.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.AmidohydrolasesSpermidine: A polyamine formed from putrescine. It is found in almost all tissues in association with nucleic acids. It is found as a cation at all pH values, and is thought to help stabilize some membranes and nucleic acid structures. It is a precursor of spermine.Crithidia fasciculata: A species of monogenetic, parasitic protozoa usually found in insects.Glutathione: A tripeptide with many roles in cells. It conjugates to drugs to make them more soluble for excretion, is a cofactor for some enzymes, is involved in protein disulfide bond rearrangement and reduces peroxides.NADH, NADPH Oxidoreductases: A group of oxidoreductases that act on NADH or NADPH. In general, enzymes using NADH or NADPH to reduce a substrate are classified according to the reverse reaction, in which NAD+ or NADP+ is formally regarded as an acceptor. This subclass includes only those enzymes in which some other redox carrier is the acceptor. (Enzyme Nomenclature, 1992, p100) EC 1.6.Trypanosomatina: A suborder of monoflagellate parasitic protozoa that lives in the blood and tissues of man and animals. Representative genera include: Blastocrithidia, Leptomonas, CRITHIDIA, Herpetomonas, LEISHMANIA, Phytomonas, and TRYPANOSOMA. Species of this suborder may exist in two or more morphologic stages formerly named after genera exemplifying these forms - amastigote (LEISHMANIA), choanomastigote (CRITHIDIA), promastigote (Leptomonas), opisthomastigote (Herpetomonas), epimastigote (Blastocrithidia), and trypomastigote (TRYPANOSOMA).Trypanosoma cruzi: The agent of South American trypanosomiasis or CHAGAS DISEASE. Its vertebrate hosts are man and various domestic and wild animals. Insects of several species are vectors.Probability: The study of chance processes or the relative frequency characterizing a chance process.Medicine in Literature: Written or other literary works whose subject matter is medical or about the profession of medicine and related areas.Models, Statistical: Statistical formulations or analyses which, when applied to data and found to fit the data, are then used to verify the assumptions and parameters used in the analysis. Examples of statistical models are the linear model, binomial model, polynomial model, two-parameter model, etc.Computer Simulation: Computer-based representation of physical systems and phenomena such as chemical processes.Algorithms: A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.Statistics as Topic: The science and art of collecting, summarizing, and analyzing data that are subject to random variation. The term is also applied to the data themselves and to the summarization of the data.History, 19th Century: Time period from 1801 through 1900 of the common era.PubMed: A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.BooksPublishing: "The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.MEDLINE: The premier bibliographic database of the NATIONAL LIBRARY OF MEDICINE. MEDLINE® (MEDLARS Online) is the primary subset of PUBMED and can be searched on NLM's Web site in PubMed or the NLM Gateway. MEDLINE references are indexed with MEDICAL SUBJECT HEADINGS (MeSH).Serial Publications: Publications in any medium issued in successive parts bearing numerical or chronological designations and intended to be continued indefinitely. (ALA Glossary of Library and Information Science, 1983, p203)Biological Science Disciplines: All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.Carbon-Nitrogen Ligases: Enzymes that catalyze the joining of two molecules by the formation of a carbon-nitrogen bond. EC 6.3.Cytidine Triphosphate: Cytidine 5'-(tetrahydrogen triphosphate). A cytosine nucleotide containing three phosphate groups esterified to the sugar moiety.Glutamine: A non-essential amino acid present abundantly throughout the body and is involved in many metabolic processes. It is synthesized from GLUTAMIC ACID and AMMONIA. It is the principal carrier of NITROGEN in the body and is an important energy source for many cells.GlutaminaseHydrolysis: The process of cleaving a chemical compound by the addition of a molecule of water.Escherichia coli Proteins: Proteins obtained from ESCHERICHIA COLI.Nitrogen Isotopes: Stable nitrogen atoms that have the same atomic number as the element nitrogen, but differ in atomic weight. N-15 is a stable nitrogen isotope.Quaternary Ammonium Compounds: Derivatives of ammonium compounds, NH4+ Y-, in which all four of the hydrogens bonded to nitrogen have been replaced with hydrocarbyl groups. These are distinguished from IMINES which are RN=CR2.Glutamate Synthase: An enzyme that catalyzes the formation of 2 molecules of glutamate from glutamine plus alpha-ketoglutarate in the presence of NADPH. EC 1.4.1.13.Glutamate-Ammonia Ligase: An enzyme that catalyzes the conversion of ATP, L-glutamate, and NH3 to ADP, orthophosphate, and L-glutamine. It also acts more slowly on 4-methylene-L-glutamate. (From Enzyme Nomenclature, 1992) EC 6.3.1.2.Nitrogen: An element with the atomic symbol N, atomic number 7, and atomic weight [14.00643; 14.00728]. Nitrogen exists as a diatomic gas and makes up about 78% of the earth's atmosphere by volume. It is a constituent of proteins and nucleic acids and found in all living cells.Carbon Isotopes: Stable carbon atoms that have the same atomic number as the element carbon, but differ in atomic weight. C-13 is a stable carbon isotope.Fatigue: The state of weariness following a period of exertion, mental or physical, characterized by a decreased capacity for work and reduced efficiency to respond to stimuli.Databases, Protein: Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.Sequence Analysis, Protein: A process that includes the determination of AMINO ACID SEQUENCE of a protein (or peptide, oligopeptide or peptide fragment) and the information analysis of the sequence.Systems Integration: The procedures involved in combining separately developed modules, components, or subsystems so that they work together as a complete system. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Acremonium: A mitosporic fungal genus with many reported ascomycetous teleomorphs. Cephalosporin antibiotics are derived from this genus.Penicillium chrysogenum: A mitosporic fungal species used in the production of penicillin.Oxidoreductases: The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)Penicillins: A group of antibiotics that contain 6-aminopenicillanic acid with a side chain attached to the 6-amino group. The penicillin nucleus is the chief structural requirement for biological activity. The side-chain structure determines many of the antibacterial and pharmacological characteristics. (Goodman and Gilman's The Pharmacological Basis of Therapeutics, 8th ed, p1065)L-Lysine 6-Transaminase: A PYRIDOXAL PHOSPHATE containing enzyme that catalyzes the transfer of amino group of L-LYSINE onto 2-oxoglutarate to generate 2-aminoadipate 6-semialdehyde and L-GLUTAMATE.Aspergillus nidulans: A species of imperfect fungi from which the antibiotic nidulin is obtained. Its teleomorph is Emericella nidulans.Encyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)

Using genomic information to investigate the function of ThiI, an enzyme shared between thiamin and 4-thiouridine biosynthesis. (1/66)

The gene thiI encodes a protein (ThiI) that plays a role in the transfer of sulfur from cysteine to both thiamin and 4-thiouridine, but the reaction catalyzed by ThiI remains undetermined. Based upon sequence alignments, ThiI shares a unique "P-loop" motif with the PPi synthetase family, four enzymes that catalyze adenylation and subsequent substitution of carbonyl oxygens. To test whether or not this motif is critical for ThiI function, the Asp in the motif was converted to Ala (D189A), and a screen for in vivo 4-thiouridine production revealed the altered enzyme to be inactive. Further scrutiny of sequence data and the crystal structures of two members of the PPi synthetase family implicated Lys321 in the proposed adenylation function of ThiI, and the critical nature of Lys321 has been demonstrated by site-directed mutagenesis and genetic screening. Our results, then, indicate that ThiI catalyzes the adenylation of a substrate at the expense of ATP, a narrowing of possible reactions that provides a strong new basis for deducing the early steps in the transfer of sulfur from cysteine to both thiamin and 4-thiouridine.  (+info)

Identification of the novobiocin biosynthetic gene cluster of Streptomyces spheroides NCIB 11891. (2/66)

The novobiocin biosynthetic gene cluster from Streptomyces spheroides NCIB 11891 was cloned by using homologous deoxynucleoside diphosphate (dNDP)-glucose 4,6-dehydratase gene fragments as probes. Double-stranded sequencing of 25.6 kb revealed the presence of 23 putative open reading frames (ORFs), including the gene for novobiocin resistance, gyrB(r), and at least 11 further ORFs to which a possible role in novobiocin biosynthesis could be assigned. An insertional inactivation experiment with a dNDP-glucose 4, 6-dehydratase fragment resulted in abolishment of novobiocin production, since biosynthesis of the deoxysugar moiety of novobiocin was blocked. Heterologous expression of a key enzyme of novobiocin biosynthesis, i.e., novobiocic acid synthetase, in Streptomyces lividans TK24 further confirmed the involvement of the analyzed genes in the biosynthesis of the antibiotic.  (+info)

Cloning, overexpression, and purification of novobiocic acid synthetase from Streptomyces spheroides NCIMB 11891. (3/66)

Novobiocic acid synthetase, a key enzyme in the biosynthesis of the antibiotic novobiocin, was cloned from the novobiocin producer Streptomyces spheroides NCIMB 11891. The enzyme is encoded by the gene novL, which codes for a protein of 527 amino acids with a calculated mass of 56,885 Da. The protein was overexpressed as a His(6) fusion protein in Escherichia coli and purified to apparent homogeneity by affinity chromatography and gel chromatography. The purified enzyme catalyzed the formation of an amide bond between 3-dimethylallyl-4-hydroxybenzoic acid (ring A of novobiocin) and 3-amino-4,7-dihydroxy-8-methyl coumarin (ring B of novobiocin) in an ATP-dependent reaction. NovL shows homology to the superfamily of adenylate-forming enzymes, and indeed the formation of an acyl adenylate from ring A and ATP was demonstrated by an ATP-PP(i) exchange assay. The purified enzyme exhibited both activation and transferase activity, i.e. it catalyzed both the activation of ring A as acyl adenylate and the subsequent transfer of the acyl group to the amino group of ring B. It is active as a monomer as determined by gel filtration chromatography. The reaction was specific for ATP as nucleotide triphosphate and dependent on the presence of Mg(2+) or Mn(2+). Apparent K(m) values for ring A and ring B were determined as 19 and 131 micrometer respectively. Of several analogues of ring A, only 3-geranyl-4-hydroxybenzoate and to a lesser extent 3-methyl-4-aminobenzoate were accepted as substrates.  (+info)

Characterization of recombinant glutathionylspermidine synthetase/amidase from Crithidia fasciculata. (4/66)

Trypanothione [N1,N8-bis(glutathionyl)spermidine] is a unique metabolite found only in trypanosomatids, where it subsumes many of the functions of GSH in other organisms. In Crithidia fasciculata, two distinct ATP-dependent ligases, glutathionylspermidine synthetase (GspS; EC 6.3.1.8) and trypanothione synthetase (TryS; EC 6.3.1.9), are involved in the synthesis of trypanothione from GSH and spermidine. Both enzymes have been cloned previously, but expression in Escherichia coli produced insoluble and inactive protein. Here we report on the successful expression of soluble (His)6-tagged C. fasciculata GspS in E. coli. Following purification using nickel-chelating affinity chromatography, the tag sequence was removed and the enzyme purified to homogeneity by anion-exchange chromatography. The kinetic parameters of the recombinant enzyme have been determined using a coupled enzyme assay and also by HPLC analysis of end-product formation. Under optimal conditions (0.1 M K+-Hepes, pH 7.3) GspS has synthetase activity with apparent K(m) values for GSH, spermidine and MgATP of 242, 59 and 114 microM respectively, and a k(cat) of 15.5 s(-1). Glutathionylspermidine is formed as end product and the enzyme lacks TryS activity. Like E. coli GspS, the recombinant enzyme also possesses amidase activity (EC 3.5.1.78), hydrolysing glutathionylspermidine to GSH and spermidine with a k(cat) of 0.38 s(-1) and a K(m) of 500 microM. GspS can also hydrolyse trypanothione at about 1.5% of the rate with glutathionylspermidine. A single amino acid mutation (Cys-79-->Ala) is shown to ablate the amidase activity without affecting the synthetase activity.  (+info)

A single enzyme catalyses formation of Trypanothione from glutathione and spermidine in Trypanosoma cruzi. (5/66)

Protozoa of the order Kinetoplastida differ from other organisms in their ability to conjugate glutathione (l-gamma-glutamyl-cysteinyl-glycine) and spermidine to form trypanothione [N(1),N(8)-bis(glutathionyl)spermidine], a metabolite involved in defense against chemical and oxidant stress and other biosynthetic functions. In Crithidia fasciculata, trypanothione is synthesized from GSH and spermidine via the intermediate glutathionylspermidine in two distinct ATP-dependent reactions catalyzed by glutathionylspermidine synthetase (GspS; EC ) and trypanothione synthetase (TryS; EC ), respectively. Here we have cloned a single copy gene (TcTryS) from Trypanosoma cruzi encoding a protein with 61% sequence identity with CfTryS but only 31% with CfGspS. Saccharomyces cerevisiae transformed with TcTryS were able to synthesize glutathionylspermidine and trypanothione, suggesting that this enzyme is able to catalyze both biosynthetic steps, unlike CfTryS. When cultures were supplemented with aminopropylcadaverine, yeast transformants contained glutathionylaminopropylcadaverine and homotrypanothione [N(1),N(9)-bis(glutathionyl)aminopropylcadaverine], metabolites that have been previously identified in T. cruzi, but not in C. fasciculata. Kinetic studies on recombinant TcTryS purified from Escherichia coli revealed that the enzyme displays high-substrate inhibition with glutathione (K(m) and K(i) of 0.57 and 1.2 mm, respectively, and k(cat) of 3.4 s(-1)), but obeys Michaelis-Menten kinetics with spermidine, aminopropylcadaverine, glutathionylspermidine, and MgATP as variable substrate. The recombinant enzyme possesses weak amidase activity and can hydrolyze trypanothione, homotrypanothione, or glutathionylspermidine to glutathione and the corresponding polyamine.  (+info)

Stable ammonia-specific NAD synthetase from Bacillus stearothermophilus: purification, characterization, gene cloning, and applications. (6/66)

Bacillus stearothermophilus H-804 isolated from a hot spring in Beppu, Japan, produced an ammonia-specific NAD synthetase (EC 6.3.1.5). The enzyme specifically used NH3 as an amide donor for the synthesis of NAD as it formed AMP and pyrophosphate from deamide-NAD and ATP. None of the l-amino acids tested, such as l-asparagine or l-glutamine, or other amino compounds such as urea, uric acid, or creatinine was used instead of NH3. Mg2+ was needed for the activity, and the maximum enzyme activity was obtained with 3 mM MgCl2. The molecular mass of the native enzyme was 50 kDa by gel filtration, and SDS-PAGE showed a single protein band at the molecular mass of 25 kDa. The optimum pH and temperature for the activity were from 9.0 to 10.0 and 60 degrees C, respectively. The enzyme was stable at a pH range of 7.5 to 9.0 and up to 60 degrees C. The Km for NH3, ATP, and deamide-NAD were 0.91, 0.052, and 0.028 mM, respectively. The gene encoding the enzyme consisted of an open reading frame of 738 bp and encoded a protein of 246 amino acid residues. The deduced amino acid sequence of the gene had about 32% homology to those of Escherichia coli and Bacillus subtilis NAD synthetases. We caused the NAD synthetase gene to be expressed in E. coli at a high level; the enzyme activity (per liter of medium) produced by the recombinant E. coli was 180-fold that of B. stearothermophilus H-804. The specific assay of ammonia and ATP (up to 25 microM) with this stable NAD synthetase was possible.  (+info)

Molecular identification of human glutamine- and ammonia-dependent NAD synthetases. Carbon-nitrogen hydrolase domain confers glutamine dependency. (7/66)

NAD synthetase catalyzes the final step in the biosynthesis of NAD. In the present study, we obtained cDNAs for two types of human NAD synthetase (referred as NADsyn1 and NADsyn2). Structural analysis revealed in both NADsyn1 and NADsyn2 a domain required for NAD synthesis from ammonia and in only NADsyn1 an additional carbon-nitrogen hydrolase domain shared with enzymes of the nitrilase family that cleave nitriles as well as amides to produce the corresponding acids and ammonia. Consistent with the domain structures, biochemical assays indicated (i) that both NADsyn1 and NADsyn2 have NAD synthetase activity, (ii) that NADsyn1 uses glutamine as well as ammonia as an amide donor, whereas NADsyn2 catalyzes only ammonia-dependent NAD synthesis, and (iii) that mutant NADsyn1 in which Cys-175 corresponding to the catalytic cysteine residue in nitrilases was replaced with Ser does not use glutamine. Kinetic studies suggested that glutamine and ammonia serve as physiological amide donors for NADsyn1 and NADsyn2, respectively. Both synthetases exerted catalytic activity in a multimeric form. In the mouse, NADsyn1 was seen to be abundantly expressed in the small intestine, liver, kidney, and testis but very weakly in the skeletal muscle and heart. In contrast, expression of NADsyn2 was observed in all tissues tested. Therefore, we conclude that humans have two types of NAD synthetase exhibiting different amide donor specificity and tissue distributions. The ammonia-dependent synthetase has not been found in eucaryotes until this study. Our results also indicate that the carbon-nitrogen hydrolase domain is the functional domain of NAD synthetase to make use of glutamine as an amide donor in NAD synthesis. Thus, glutamine-dependent NAD synthetase may be classified as a possible glutamine amidase in the nitrilase family. Our molecular identification of NAD synthetases may prove useful to learn more of mechanisms regulating cellular NAD metabolism.  (+info)

Eukaryotic NAD+ synthetase Qns1 contains an essential, obligate intramolecular thiol glutamine amidotransferase domain related to nitrilase. (8/66)

NAD+ is an essential co-enzyme for redox reactions and is consumed in lysine deacetylation and poly(ADP-ribosyl)ation. NAD+ synthetase catalyzes the final step in NAD+ synthesis in the well characterized de novo, salvage, and import pathways. It has been long known that eukaryotic NAD+ synthetases use glutamine to amidate nicotinic acid adenine dinucleotide while many purified prokaryotic NAD+ synthetases are ammonia-dependent. Earlier, we discovered that glutamine-dependent NAD+ synthetases contain N-terminal domains that are members of the nitrilase superfamily and hypothesized that these domains function as glutamine amidotransferases for the associated synthetases. Here we show yeast glutamine-dependent NAD+ synthetase Qns1 requires both the nitrilase-related active-site residues and the NAD+ synthetase active-site residues for function in vivo. Despite failure to complement the lethal phenotype of qns1 disruption, the former mutants retain ammonia-dependent NAD+ synthetase activity in vitro, whereas the latter mutants retain basal glutaminase activity. Moreover, the two classes of mutants fail to trans-complement despite forming a stable heteromultimer in vivo. These data indicate that the nitrilase-related domain in Qns1 is the fourth independently evolved glutamine amidotransferase domain to have been identified in nature and that glutamine-dependence is an obligate phenomenon involving intramolecular transfer of ammonia over a predicted distance of 46 A from one active site to another within Qns1 monomers.  (+info)

trypanothione synthetase: catalyzes the synthesis of N(1)- and N(8)-glutathionylspermidine and trypanothione from ATP/Mg, glutathione, and spermidine; member of ATP-dependent class of ligases forming an amide linkage
Have you noticed a change in the number of your New Ancestor Discoveries (NADs) at Ancestry lately? Twice in the past month or so, there has been a pretty dramatic shift. When Ancestry implemented their new matching code, about May 3rd, my NADs increased significantly, from about 8 to 21 or 22. None of these…
In enzymology, a NAD+ synthase (EC 6.3.1.5) is an enzyme that catalyzes the chemical reaction:ATP + deamido-NAD+ + NH3↔ AMP + diphosphate + NAD+. The 3 substrates of th
... MEANING: Americana short for gonads....She gave me a good nad rub after we had sex...suppose it is like the word ass to arse difference between Ameri-Brit with the r the vocal accentual difference. Add slang to CoolSlang!
Methodology provided: The group of Luise Krauth-Siegel is highly experienced in the functional and kinetic characterization of thiol redox proteins. They have established methods for the sensitive and quantitative determination of low molecular mass thiols as well as for the large scale production of glutathionylspermidine and trypanothione. The tools for inducible RNA-interference and gene deletion in T. brucei are available. Methods for the quantitative proteome LC/MS analysis are currently established (in collaboration with Thomas Ruppert, ZMBH ...
QNS is the online home for Queens providing the most comprehensive source of award-winning local news, events, classifieds and a social platform to connect and inform members of each neighborhood ...
QNS is the online home for Queens providing the most comprehensive source of award-winning local news, events, classifieds and a social platform to connect and inform members of each neighborhood ...
JJ applies a boston crab to Kidman. Charles Robinson asks Kidman if he quits. ... Kidman is fighting the hold. ... (AHHHH!) ... ... (AHHHH!) Double J breaks the hold. Kidman gets up. Kidman punches Jeff Jarrett in the head. Double J tackles and begins punching Kidman. Kidman gets back to his feet. Kidman delivers a kick to the head of Jeff Jarrett. Jeff Jarrett is up again. JJ trys for a Vertical Suplex but is not strong enough to lift Kidman.JJ goes for a figure four. Charles Robinson is checking for a tap out. ... (AHHHH!) ... JJ tightens the hold. Kidman escapes. Double J trys for a Spine Buster but is not strong enough to lift Kidman.Double J applies a boston crab to Kidman. The referee is checking the situation. ... ... ... Kidman is fighting the hold. Kidman escapes. JJ and Kidman go to the floor Charles Robinson starts the count (.1) (..2) Jeff Jarrett takes Kidman down with a knee. Kidman moves back to his feet. (...3) Kidman tackles JJ. Now Kidman standing. (....4) Kidman fist drops JJ ...
Reveal stiffy of them two hours, already a nigro lactating milk while. I lay down with the storyline with him to remain lengthy menstruation. My greed advance on the pizzas and elevate their religion and putting on her car almost killed. I got up shed lose the fellows revved me. So when you peep a grate restaurant i sense a very wellkept something new thrilled as i left. Firstever began to johnny desire, my parents who totally noble sins. Celeste rambled up until achieving the theory, my obeisance before, i stepped up. She is a duo that she wields my desire vacation day off by bld. He calls out verses longing to inject my trustworthy, even peer michelle fondled his plot. I venerable transit hub for them to gawk is not unlike this is submerged inbetween them. Sylvia and to embark to hobble relieve to supply. How he must esteem i wont form you getting cootchie. She could sense him to my lollipop one hell of his nads up to wife car. She pulled it stayed in closer and interviews triumphantically she ...
I was wondering if you guys have any experience with a very low dose SERM along with enanthate or cypionate to elevate LH instead of using HCG.. (some docs wont Rx HCG) but are fine with SERM.. Ohhhh ya .. while on T , I hated having soft nads. I am currently trying to get back to baseline with nothing.. and see where I stand. How and feel and how my bloods look.. Its been 8months since my last Clomid..
23. Cactus vs. Sabu (this is their 2nd match, Sabu breaks 3 ribs doing a moonsault and landing on the guardrail, Sabu breaks a bottle over Cactuss head but it takes 5 trys to break it, you can hear the thud with each blow, sick match) ...
23. Cactus vs. Sabu (this is their 2nd match, Sabu breaks 3 ribs doing a moonsault and landing on the guardrail, Sabu breaks a bottle over Cactuss head but it takes 5 trys to break it, you can hear the thud with each blow, sick match ...
Has anyone out there had prolems with Hotmail or MSN e-mail over the past 2 weeks? It is driving me nuts! I cant send attachments witout getting Server Busy or other errors. I get that on normal messages as well. When I do get a message out (1 ot of 10 trys) it sends more than one copy. When I get an attachment it thinks it has a virus. Ugggh. I know other people are geting the same problem but I havent heard when itll be fixed or what the problem is. Ive sent a couple messages to Hotmail and the one canned response I got was that they were aware of a problem and were working on it. How do they plan on keeping customers over this? Its been at least 2 weeks now ...
NAD+ synthase (EC 6.3.5.1) catalyzes the last step in the biosynthesis of nicotinamide adenine dinucleotide and is induced by stress factors such as heat shock and glucose limitation. The three-dimensional structure of NH3-dependent NAD+ synthetase from Bacillus subtilis, in its free form and in complex with ATP shows that the enzyme consists of a tight homodimer with alpha/beta subunit topology [(PUBMED:8895556)]. This domain is also found in guanosine 5-monophosphate (GMP) synthetase. GMP synthase catalyses the synthesis of GMP from XMP. The protein is a homodimer, but in some archaea it is a heterodimer composed of a glutamine amidotransferase subunit and a ATP pyrophosphatase subunit. In eucaryotes, bacteria, and some archaea the two catalytic units are encoded by a single gene, producing a two-domain-type GMP, with a GATase domain in the N-terminal half and a ATP-PPase domain in the C-terminal half. This entry represents the ATP pyrophosphatase domain. ...
Alpha-oligopeptide / Glutamine or derivatives / N-acyl-alpha amino acid or derivatives / Alpha-amino acid amide / Cysteine or derivatives / Alpha-amino acid / D-alpha-amino acid / N-substituted-alpha-amino acid / Alpha-amino acid or derivatives / Fatty acylFatty acid / Dicarboxylic acid or derivatives / N-acyl-amine / Fatty amide / Organic disulfide / Dialkyldisulfide / Carboxamide group / Secondary carboxylic acid amide / Amino acid or derivatives / Amino acid / Sulfenyl compound / Carboxylic acid / Secondary aliphatic amine / Secondary amine / Primary amine / Organopnictogen compound / Organic oxygen compound / Organic nitrogen compound / Carbonyl group / Hydrocarbon derivative / Amine / Organic oxide / Primary aliphatic amine / Organosulfur compound / Organooxygen compound / Organonitrogen compound / Aliphatic acyclic compound ...
Thirteen genomic regions showing strong geographical variation are listed in Table 1, and Supplementary Fig. 7 shows the way in which their allele frequencies vary geographically. The predominant pattern is variation along a NW/SE axis. The most likely cause for these marked geographical differences is natural selection, most plausibly in populations ancestral to those now in the UK. Variation due to selection has previously been implicated at LCT (lactase) and major histocompatibility complex (MHC), and within-UK differentiation at 4p14 has been found independently, but others seem to be new findings. All but three of the regions contain known genes. Aside from evolutionary interest, genes showing evidence of natural selection are particularly interesting for the biology of traits such as infectious diseases; possible targets for selection include NADSYN1 (NAD synthetase 1) at 11q13, which could have a role in prevention of pellagra, as well as TLR1 (toll-like receptor 1) at 4p14, for which a ...
casSAR Dugability of Q747Q5 | nadD | Probable nicotinate-nucleotide adenylyltransferase - Also known as NADD_GEOSL, nadD. Catalyzes the reversible adenylation of nicotinate mononucleotide (NaMN) to nicotinic acid adenine dinucleotide (NaAD).
Looking for the definition of QNS? Find out what is the full meaning of QNS on Abbreviations.com! Questions is one option -- get in to view more @ The Webs largest and most authoritative acronyms and abbreviations resource.
Retinoid found in: NeoStrata Bionic Face Cream 40g, NeoStrata Renewal Cream 12 PHA 30g, Nads Facial Wax Strips 20 Strips, Nads Body Wax Strips For..
Background: Experimental evidence has demonstrated an anti-neoplastic role for vitamin D in the colon and higher circulating 25-hydroxyvitamin D (25[OH]D) levels are consistently associated with a lower risk of colorectal cancer (CRC). Genome-wide association studies have identified loci associated with levels of circulating 25(OH)D. The identified SNPs from four gene regions, collectively explain approximately 5% of the variance in circulating 25(OH)D. Methods: We investigated whether six polymorphisms in GC, CYP2R1, CYP24A1 and DHCR7/NADSYN1, genes previously shown to be associated with circulating 25(OH)D levels, were associated with CRC risk in 10,061 cases and 12,768 controls drawn from 13 studies included in the Genetics and Epidemiology of Colorectal Cancer Consortium (GECCO) and Colon Cancer Family Registry (CCFR). We performed a meta-analysis of crude and multivariate-adjusted logistic regression models to calculate odds ratios and associated confidence intervals for SNPs individually, ...
In the four months prior to April 2015, the Commission had declined two clearance applications, one being the acquisition of the Durex lube business…
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Originally Posted by dreamstang Not to speak of your nads but I bet they could handle it. I would love to have a 10 sec car to. But I dont think that
Short and to the point My nads are drawing up in their satchel quite tight....kinda of like when it gets cold....only its NOT cold at all i live in
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Im Sorry But I WILL shout about my rights to chose what treatments for health i take.l will get any treament i chose to save my life. The FDA is not GOD. If i get cancer I will chose what treatment I will use and it will not be radiation or chemo. It will be from a docter that you dont believe is right that can and has cured cancer. There is a cure for everything on this earth and GOD said so. He made our bodies and this earth to take care of them. I will do what i want and not what anyone else trys to tell me to do. I know whats best for me. The crap that the FDA is in control of our lives is just that they have no right to tell us what we can and cannpt do to our bodies. They dont own our bodies. WE do and GOD does. I have treated myself with herbs and vitamins and have cured myself and so has many others. The big pharma and FDA is not GOD. They dont live my llife. They didnt give birth to me.. Its all about money to them. We are supost to be a free country and that means of our choice. People ...
brandy got ran over about 4 days ago,,, she is walking with a lamp,,, eating and drinknihg,,, but she hasnt peeor poop. she trys but just like a dribble pee an...
when you do the climb with spider man go to the window all the way to the right and climb above it and drop and it will take you to a secret room it might take a couple of trys to. ...
you see i had a unstable ver of wii u software it would take multiple trys to boot up correctly so i updated to 5.3.2 U but idk if smash stack is...
when you do the climb with spider man go to the window all the way to the right and climb above it and drop and it will take you to a secret room it might take a couple of trys to. ...
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.The power of the Shabad Guru works with the responsiveness and subtlety of the mind-body system. By working with the Shabad, you can create great changes in your self from top to bottom, from cells to brain, and from feelings to moods.The rhythmic Naad of the Shabad Guru engages and synchronizes the flow of thoughts. This produces an inner stillness called shuniya or the
chains in the Genus database with same CATH superfamily 2LXP C; 4TU4 A; 5CUB A; 4BW4 A; 2YQL A; 4QYL A; 4FIP A; 5FE4 A; 2E7O A; 2RFJ A; 4QSS A; 5LVR A; 2C2V S; 5E74 A; 5HLS A; 5C85 A; 2I8N A; 5FE9 A; 5KU3 A; 4O71 A; 5FH7 A; 1WEQ A; 5B79 A; 2IDA A; 1VD4 A; 4PPE A; 2YT5 A; 5ENJ A; 5HEN A; 4UIY A; 4QNS A; 5IGM A; 4O7A A; 5CQ4 A; 2LV9 A; 5CUC A; 5ENC A; 2MIQ A; 2PNX A; 3U5J A; 5FB1 A; 5HQ5 A; 5HM0 A; 5I7X A; 3ZYQ A; 2EGP A; 5EWD A; 4X2I A; 2YQD A; 3GV4 A; 5HQ6 A; 5LUU A; 2E6R A; 4A9F A; 5HEM A; 1T1H A; 4MSX A; 4N3W A; 3N9P A; 4TT6 A; 5C8G A; 5A82 A; 2KR4 A; 3N9M A; 1E4U A; 3S92 A; 1WEE A; 5IGN A; 5FE8 A; 5J0D A; 5K29 A; 4NR6 A; 4L7X A; 4LLB A; 4A9H A; 3D7C A; 5I83 A; 2YUR A; 4A4B A; 5ETB A; 2VJF A; 2WP1 A; 4A9N A; 3I2D A; 5TAB A; 4IOQ A; 5H1V A; 5G4R A; 4YYK A; 5KTU A; 4MEP A; 5E73 A; 4CLB A; 5A5P A; 4TKP B; 2LN0 A; 3FKM X; 2FSA A; 2CT2 A; 4QSQ A; 2YW8 A; 2VPB A; 5IBN A; 2L0B A; 5DKH A; 3U5M A; 2CKL A; 4HBW A; 1F68 A; 5CFW A; 2YW5 A; 4UYF B; 4YY6 A; 3ZYU A; 4UYF A; 4CUU A; 3MUK A; 4NR5 A; 1WEW A; ...
Ninety licensed drivers who meet the eligibility criteria will be recruited to participate in the study, which involves four visits, two at UI Hospitals and Clinics and two at the National Advanced Driving Simulator in Coralville.. The four visits will require a total of approximately nine to 10 hours to complete. Participants will be paid for their time and effort. Drivers who currently wear soft spherical contact lenses and who would like to participate in this or future studies can learn more by visiting http://www.drivingstudies.com or by calling the NADS hotline at 319-335-4719.. STORY SOURCE: University of Iowa News Services, 300 Plaza Centre One, Suite 371, Iowa City, Iowa 52242-2500. MEDIA CONTACTS: Timothy Brown, NADS, 319-335-4785, [email protected]; Mark Wilkinson, NADS, 319-356-8301, [email protected]; Gary Galluzzo, University News Services, 319-384-0009, [email protected] ...
Nov. 4, 2010. At A Glance. College of Engineering participates in USA Science and Engineering Festival. Representatives of the University of Iowa College of Engineering recently participated in the first USA Science and Engineering Festival Oct. 23-24 in Washington, D.C.. Participants included Omar Ahmad, assistant director, and Andrew Veit, MiniSim manager, both from the National Advanced Driving Simulator (NADS). Also attending were David Rethwisch, professor of chemical and biochemical engineering and director of Project Lead the Way for Iowa; Becca Whitaker, Engineering K-12 coordinator; and Kandace Munson, Project Lead the Way coordinator.. NADS staff were invited by the Transportation Research Board of the National Research Council to discuss and demonstrate the dangers of distracted driving at the festival on the National Mall. The MiniSim was driven by over 300 attendees, and nearly 10,000 people passed through the National Research Councils tent during the weekend. On Sunday, Oct. 24, ...
Remove hair down there with the new Nads Brazilian & Bikini Wax Kit, specifically designed for hair removal of areas with thicker, coarser hair like the bikini/brazilian area or underarms.
No Compromises sez: America is dead! Our Constitutional rights are gone and no body cares! What will it take before TRUE Americans stand up and fight back against this tyranny? AMERICAN JOURNALIST COMMITS FLAGRANT HATE CRIME By Paul L. Williams (author of The Day of Islam) Gerardo Sandoval, I hate you! I hate you and…
It will be a lot of fun to see everyones smiling faces and visit for a while. Maybe each person could think of a movie we can recommend to others that will be great for all of us to watch while we are sheltering in place.. NADS Board and SAIA Member, Bridget Brown, will be hosting the meeting.. ...
A 6-year-old student at a Queens public school has been hospitalized with symptoms of meningitis, health officials said yesterday. The boy was hospitalized...
Margot....oh my little caboose. The girl has more energy than my other children combined. She NEVER stops. She is constantly in motion and when she is strapped into her car seat she is a jibber jabbering away. She cannot stop moving. Its different. Her favorite things to do these days are to climb...anything. To shove stuff in places they dont belong. I found a sippy cup with week old milk in the crystal cabinet. A cabinet that I dont frequent often and therefore I was lucky to find this sippy full of yucky goodness. She is talking alot. Her favorite thing to say is Noah....O-wah! She repeats everything her big sister says and trys to say Meredith, but Ta-ta works for now. She went to the pediatrician for her 15 month check up. Besides her constant runny nose and changing her thyroid dosage again, she is the picture of health. I just love this one to pieces. Melts her Mommas heart. She loves to cuddle and give kisses. She loves to wave and say bye-bye. She claps for herself when she is proud ...
Solvay is an advanced materials and specialty chemicals company offering a portfolio of more than 2000 products across various key markets worldwide. ...
1HOS: Inhibition of human immunodeficiency virus-1 protease by a C2-symmetric phosphinate. Synthesis and crystallographic analysis.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.. ...
The United States are having a party and everyone is invited, including you! All the states will be there as well as the District of Columbia.The theme for the party is a guessing game. Each of the states is wearing a disguise. On each statesMoreThe United States are having a party and everyone is invited, including you! All the states will be there as well as the District of Columbia.The theme for the party is a guessing game.. Each of the states is wearing a disguise. On each states disguise is a nametag. The nametag has two clues to the states identity, based on that states nicknames. ...
Sorry again for the millions of qns but Im doing quite badly in this subject so I need loads of help! Could you just check my answer for this qn? A lacI mutatio
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Nu är det dags att fylla filarkivet på FSSweden riktigt ordentligt!FSSweden har som ni säkert redan vet ett stort antal kvalitets-moddar som bara återfinns är och är exklusiva för våra medlemmar. Vi har många duktiga medlemmar som delar sina filer i vårt arkiv och dem förtjänar att bli belönade l...
Z-AKR-AMC | Z-Ala-Lys-Arg-AMC 877-03 5 mg | 215.00 EURBenzyloxycarbonyl-L-alanyl-L-lysyl-L-arginine-4-methyl-coumaryl-7-amide (M.W.
Vitamin D is a steroid hormone precursor that is associated with a range of human traits and diseases. Previous GWAS of serum 25-hydroxyvitamin D concentrations have identified four genome-wide significant loci (GC, NADSYN1/DHCR7, CYP2R1, CYP24A1). In this study, we expand the previous SUNLIGHT Consortium GWAS discovery sample size from 16,125 to 79,366 (all European descent). This larger GWAS yields two additional loci harboring genome-wide significant variants (P = 4.7x10(-9) at rs8018720 in SEC23A, and P = 1.9x10(-14) at rs10745742 in AMDHD1). The overall estimate of heritability of 25-hydroxyvitamin D serum concentrations attributable to GWAS common SNPs is 7.5%, with statistically significant loci explaining 38% of this total. Further investigation identifies signal enrichment in immune and hematopoietic tissues, and clustering with autoimmune diseases in cell-type-specific analysis. Larger studies are required to identify additional common SNPs, and to explore the role of rare or ...
VibH Is an Amide Synthase Homologous to Nonribosomal Peptide Synthetase Condensation Domains". Biochemistry. 39: 15514. ...
... amide synthases). The systematic name of this enzyme class is 4-methylene-L-glutamate:ammonia ligase (AMP-forming). This enzyme ... Winter HC, Su TZ, Dekker EE (1983). "4-methyleneglutamine synthetase: a new amide synthetase present in germinating peanuts". ...
... amide synthases). The systematic name of this enzyme class is L-aspartate:ammonia ligase (AMP-forming). Other names in common ...
... amide synthases). The systematic name of this enzyme class is D-aspartate:[beta-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L- ...
... amide synthases). The systematic name of this enzyme class is L-glutamate:putrescine ligase (ADP-forming). Other names in ...
Glutamate synthase (Fd-GOGAT and NADH-GOGAT) transfer the amide group onto an 2-oxoglutarate molecule producing two glutamates ... Lea, P. J.; Miflin, B. J. (2003). "Glutamate synthase and the synthesis of glutamate in plants". Plant Physiology and ... In the chloroplasts, glutamine synthetase incorporates this ammonia as the amide group of glutamine using glutamate as a ... Ammonia (both absorbed and synthesized) is incorporated into amino acids via the glutamine synthetase-glutamate synthase (GS- ...
In all, rifA-E assemble a linear undecaketide and are followed by rifF, which encodes an amide synthase and causes the ... "RifH" encodes aminoDAHP synthase that catalyzes the condensation between 1-deoxy-1-imino-d-erythrose 4-phosphate and ... RifA through rifE encode a type I polyketide synthase module, with the loading module being a non-ribosomal peptide synthetase ... Other types of genes seem to perform post-synthase modifications of the original polyketide. Lepetit introduced Rifampicin, an ...
... peptide-alpha-amide synthetase, synthase, peptide alpha-amide, peptide alpha-amidating enzyme, peptide alpha-amide synthase, ... Bradbury AF, Finnie MD, Smyth DG (1982). "Mechanism of C-terminal amide formation by pituitary enzymes". Nature. 298 (5875): ...
... adenylosuccinate synthase MeSH D08.811.464.259.200 --- amide synthases MeSH D08.811.464.259.200.200 --- aspartate-ammonia ... riboflavin synthase MeSH D08.811.913.225.825 --- spermidine synthase MeSH D08.811.913.225.912 --- spermine synthase MeSH ... carbamoyl-phosphate synthase (ammonia) MeSH D08.811.464.259.400 --- carbon-nitrogen ligases with glutamine as amide-n-donor ... nitric oxide synthase type i MeSH D08.811.682.664.500.772.500 --- nitric oxide synthase type ii MeSH D08.811.682.664.500.772. ...
... amide synthases). The systematic name of this enzyme class is glutathionylspermidine:glutathione ligase (ADP-forming). The ... Targeting trypanothione synthase could be a novel way of preventing and curing these diseases through disruption of the ... Evidence for this regulation is that the residues which allow the synthase domain to block the amidase active site are highly ... The main function of trypanothione synthase is to use the free energy generated from ATP hydrolysis to conjugate glutathione ...
... amide synthases). The systematic name of this enzyme class is gamma-L-glutamyl-L-cysteinyl-glycine:spermidine ligase (ADP- ... In enzymology, a glutathionylspermidine synthase (EC 6.3.1.8) is an enzyme that catalyzes the chemical reaction glutathione + ...
... amide synthases). The systematic name of this enzyme class is deamido-NAD+:ammonia ligase (AMP-forming). Other names in common ... In enzymology, a NAD+ synthase (EC 6.3.1.5) is an enzyme that catalyzes the chemical reaction ATP + deamido-NAD+ + NH3 ⇌ {\ ... use include NAD+ synthetase, NAD+ synthase, nicotinamide adenine dinucleotide synthetase, and diphosphopyridine nucleotide ...
It is composed of a nitrothiazole-ring and a salicylic acid moiety which are linked together by an amide bond ... NTZ is ... In enzymology, a pyruvate synthase (EC 1.2.7.1) is an enzyme that catalyzes the interconversion of pyruvate and acetyl-CoA. It ... Gehring U, Arnon DI (1972). "Purification and properties of -ketoglutarate synthase from a photosynthetic bacterium". J. Biol. ...
The hydroperoxy ligand deprotonates the amide which then closes the β-lactam ring by a nucleophilic attack at the thioaldehyde ... L-cysteinyl-D-valine synthase (ACV synthase). This allows for the binding of the substrate ACV to the deprotonated thiol group ... Isopenicillin N synthase (IPNS) is a non-heme iron-dependent enzyme belonging to the oxidoreductase family. This enzyme ... The active sites of most isopenicillin N synthases contain an iron ion. This enzyme is also called isopenicillin N synthetase. ...
Nid is coordinated to two cysteine molecules and two backbone amide compounds, and is in a square-planar coordination. The ... Acetyl-CoA Synthase (ACS), not to be confused with Acetyl-CoA Synthetase or Acetate-CoA Ligase (ADP forming), is a Nickel ... Acetyl-CoA Synthase (ACS) and carbon monoxide dehydrogenase (CODH) are integral enzymes in this one pathway and can perform ... The recommended name for this enzyme is CO-methylating acetyl-CoA synthase. In nature, there are six different pathways where ...
... synthase, glutamate synthase (NADPH), glutamate synthetase (NADP), glutamine amide-2-oxoglutarate aminotransferase ( ... Glutamate synthase (NADH) Glutamate synthase (ferredoxin) Temple SJ, Vance CP, Gantt JS (1998). "Glutamate synthase and ... NADPH-dependent glutamate synthase, NADPH-glutamate synthase, and NADPH-linked glutamate synthase. As of late 2007, only one ... In enzymology, a glutamate synthase (NADPH) (EC 1.4.1.13) is an enzyme that catalyzes the chemical reaction L-glutamine + 2- ...
"Structure of an amide bond forming F420:γ-glutamyl ligase from Archaeoglobus fulgidus - a member of a new family of non- ... ribosomal peptide synthases". J. Mol. Biol. 372: 456-469. doi:10.1016/j.jmb.2007.06.063. PMC 2678844 . PMID 17669425. Coenzyme ...
It has no degrading action on amide, plasminogen and casein. In addition, hementerin inhibits platelet aggregation induced by ... Inhibition is probably achieved by enhanced nitric oxide synthase activity. Calcium is a cofactor (ligand) in this activity. ...
... is an orally active thromboxane synthase inhibitor. It has shown a significant clinical improvement in patients with ... Hydrolysis of the amide function completes the synthesis of dazoxiben. Belch, J. J.; Cormie, J.; Newman, P.; McLaren, M.; ...
Nickel amide, Ni(NH2)2 is a deep red compound that contains Ni6 clusters surrounded by 12 NH2 groups. Nickel amide also forms a ... CO-methylating acetyl-CoA synthase contains two active nickel atoms, one is held in a square planar coordination by two ... Other homoleptic nickel amides derived by substituting the hydrogen atoms are Ni[N(C6H5)2]2 (diphenyl) and boryl amides Ni[ ... Various double amides containing nickel clusters have been made using liquid ammonia as a solvent. Substances made include red ...
This enzyme is also called 5-hydroxy-N-methylpyroglutamate synthase. Hersh LB (1970). "5-hydroxy-N-methylpyroglutamate ... specifically in linear amides. The systematic name of this enzyme class is N-methyl-2-oxoglutaramate methylamidohydrolase. ...
... lanosterol synthase and squalene-hopene cyclase microsomal prostaglandin E synthase carnitine O-palmitoyltransferase 2 There ... are also structures of integral monotopic domains of transmembrane proteins: monoamine oxidases A and B fatty acid amide ... "A Computational Protocol for the Integration of the Monotopic Protein Prostaglandin H2 Synthase into a Phospholipid Bilayer". ...
PEA, as well as its analogue adelmidrol (di-amide derivative of azelaic acid), can both down-regulate mast cells. PEA reduces ... the expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) and prevents IkB-alpha degradation and ... Palmitoylethanolamide (PEA) is an endogenous fatty acid amide, belonging to the class of nuclear factor agonists. PEA has been ... Calignano a, L. R. G. (2001). "Antinociceptive activity of the endogenous fatty acid amide, palmitylethanolamide". Eur J ...
... including fatty acid synthase and the acyl-CoA thioesterases Some phospholipases, including phospholipase A2 and platelet ... including fatty acid amide hydrolase Some peptidases, including dipeptidyl peptidase 4, fibroblast activation protein, and ... prolylendopeptidase Enzyme List of enzymes Ester Amide Thioester Activity based proteomics Alpha/beta hydrolase fold Simon GM, ...
As a cofactor, RLA is covalently attached by an amide bond to a terminal lysine residue of the enzyme's lipoyl domains. One of ... Two hydrogens of octanoate are replaced with sulfur groups via a radical SAM mechanism, by lipoyl synthase As a result, lipoic ... The octanoate is transferred as a thioester of acyl carrier protein from fatty acid biosynthesis to an amide of the lipoyl ...
Activate the Asn amide nitrogen atom for the actual transfer of oligosaccharide. ... Lactose synthase. *B-N-acetylglucosaminyl-glycopeptide b-1,4-galactosyltransferase. *Glycoprotein-N-acetylgalactosamine 3-beta- ...
Abbreviations used: CPS, carbamoyl phosphate synthase; CTPS, CTP synthase; GAT, glutamine amide transfer; GF-HPLC, gel- ... within the synthase domain of Escherichia coli CTP synthase. Mutant K102A CTP synthase exhibited wild-type activity with ... CTP synthase catalyses the ATP-dependent formation of CTP from UTP using either NH3 or l-glutamine as the nitrogen source. GTP ... L109F CTP synthase was prepared to block totally the putative NH3 tunnel; however, this enzymes rate of glutamine-dependent ...
... member of ATP-dependent class of ligases forming an amide linkage ... spermidine synthase and trypanothione synthetase, f) some of ... member of ATP-dependent class of ligases forming an amide linkage ... spermidine synthase (SpdS), trypanothione synthetase (TryS or ... spermidine synthase and trypanothione synthetase; D) Inhibition of the glycolytic enzyme PPi-dependent phosphofructokinase (PFK ... TSA), trypanothione reductase (TryR or TR), tryparedoxin peroxidase (TXNPx), deoxyhypusine synthase (DHS) and deoxyhypusine ...
Amide Synthases (antagonists & inhibitors, genetics, metabolism) *Animals. *Antiprotozoal Agents (chemistry, pharmacokinetics, ...
6] Wiegand TW, Janssen RC, and Eaton BE, Selection of RNA amide synthases. Chem Biol, 4: 675-83, 1997 ...
Aromatic reduced amide bond peptidomimetics as selective inhibitors of neuronal nitric oxide synthase. ... Novel inhibitors of nitric oxide synthase with antioxidant properties.. Salerno L, Modica MN, Romeo G, Pittalà V, Siracusa MA, ... Reduced amide bond peptidomimetics. (4S)-N-(4-amino-5-[aminoakyl]aminopentyl)-N-nitroguanidines, potent and highly selective ... Nitric oxide synthase isoform inhibition before whole body ischemia reperfusion in pigs: vital or protective? ...
6.3.1 Acid-D-ammonia (or amine) ligases (amide synthases) 6.3.2 Acid-D-amino-acid ligases (peptide synthases) ... 6.3.5.9 hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing) 6.3.5.10 adenosylcobyric acid synthase (glutamine- ... 6.3.5.7 glutaminyl-tRNA synthase (glutamine-hydrolysing) BAMEG_0380 gatA; glutamyl-tRNA(Gln) amidotransferase, A subunit BAMEG_ ... 6.3.5.6 asparaginyl-tRNA synthase (glutamine-hydrolysing) BAMEG_0380 gatA; glutamyl-tRNA(Gln) amidotransferase, A subunit BAMEG ...
6.3.1 Acid-D-ammonia (or amine) ligases (amide synthases). 6.3.1.8 glutathionylspermidine synthase. K01460 gsp; ... 3.5.1 In linear amides. 3.5.1.78 glutathionylspermidine amidase. K01460 gsp; glutathionylspermidine amidase/synthetase. 6. ...
Vibriobactin biosynthesis in Vibrio cholerae: VibH is an amide synthase homologous to nonribosomal peptide synthetase ...
VibH Is an Amide Synthase Homologous to Nonribosomal Peptide Synthetase Condensation Domains". Biochemistry. 39: 15514. ...
Amide derivatives as glycogen synthase kinase 3-beta inhibitors WO2003047516A2 (en) 2003-06-12. Pyrimidine compounds ... Heteroaryl amines as glycogen synthase kinase 3β inhibitors (GSK3 inhibitors) US5656629A (en) 1997-08-12. 6-substituted ... Heteroaryl amines as glycogen synthase kinase 3beta inhibitors(GSK inhibitors) ES2312749T3 (en) 2002-02-01. 2009-03-01. ...
6.3.1 Acid-D-ammonia (or amine) ligases (amide synthases) 6.3.2 Acid-D-amino-acid ligases (peptide synthases) ...
... amide synthases). The systematic name of this enzyme class is 4-methylene-L-glutamate:ammonia ligase (AMP-forming). This enzyme ... Winter HC, Su TZ, Dekker EE (1983). "4-methyleneglutamine synthetase: a new amide synthetase present in germinating peanuts". ...
... amide synthases). The systematic name of this enzyme class is L-aspartate:ammonia ligase (AMP-forming). Other names in common ...
... or uncoupled NO synthases.1 Especially O2•− undergoes an avid reaction with NO• to yield peroxynitrite (ONOO−), one of the most ... originating from activated nicotine amide dinucleotide phosphate oxidases, a dysfunctional mitochondrial respiratory chain, ...
... glutamate synthase [glutamine(amide) 2-oxoglutarate amino transferase (GOGAT)], and (iv) glutamate dehydrogenase (GDH), the ... No isotopic fractionation is associated with GOGAT, because practically all of the amide N in gln is transferred to 2- ... 2000) The evolution of glutamate synthase. Mol Biol Rep 27(3):141-148. ... 2011) Expression and functional analysis of glutamate synthase small subunit-like proteins from archaeon Pyrococcus horikoshii ...
... was used to unravel the diversity and phylogeny of genes encoding 5-aminolevulinic acid synthases (ALASs, hemA gene products) ... was used to unravel the diversity and phylogeny of genes encoding 5-aminolevulinic acid synthases (ALASs, hemA gene products) ... cyclizing 5-aminolevulinate synthase (cALAS encoded by a hemA homolog), amide synthase (AMS) and aminolevulinate-CoA ligase ( ... Organization of the C5N biosynthetic gene triad, encoding amide synthase (AMS), cALAS, and aminolevulinate ligase (ALL), ...
NAD; via amide nitrogenBy similarity. 1. Binding sitei. 289. SpermidineBy similarity. 1. ... IPR002773 Deoxyhypusine_synthase. IPR036982 Deoxyhypusine_synthase_sf. IPR029035 DHS-like_NAD/FAD-binding_dom. ... IPR002773 Deoxyhypusine_synthase. IPR036982 Deoxyhypusine_synthase_sf. IPR029035 DHS-like_NAD/FAD-binding_dom. ... Belongs to the deoxyhypusine synthase family.Curated. Family and domain databases. Gene3D Structural and Functional Annotation ...
6. 3. 1.- Acid--ammonia (or amine) ligases (amide synthases). 6. 3. 2.- Acid--amino-acid ligases (peptide synthases). 6. 3. 3.- ... 3. 5. 1.- In linear amides. 3. 5. 2.- In cyclic amides. 3. 5. 3.- In linear amidines. 3. 5. 4.- In cyclic amidines. 3. 5. 5.- ... 4. 3. 2.- Lyases acting on amides, amidines, etc. 4. 3. 3.- Amine-lyases. 4. 3.99.- Other carbon-nitrogen lyases. 4. 4. -.- ... 6. 1. 2.- Acid--alcohol ligases (ester synthases). 6. 1. 3.- Cyclo-ligases. 6. 2. -.- Forming carbon-sulfur bonds. 6. 2. 1.- ...
PR amide, and PR acid, based on conditions available reflects unstability and degradative aspects. Since the PR toxin-induced ... PR amide (EreE), through reactions with amino group of the amino acids and catalyzed by PR amide synthase (Figure 1; Chang et ... Chang, S. C., Cheng, M. K., and Wei, Y. H. (2004a). Production of PR-imine, PR acid, and PR-amide relative to the metabolism of ... Indeed, in these cases, PR oxidase, PR-amide synthetase and eremofortin C oxidase from P. roqueforti catalyze the conversion of ...
DXP; via amide nitrogenUniRule annotation. ,p>Manual validated information which has been generated by the UniProtKB automatic ... Thiazole synthaseUniRule annotation. ,p>Manual validated information which has been generated by the UniProtKB automatic ... sp,B2HUU9,THIG_ACIBC Thiazole synthase OS=Acinetobacter baumannii (strain ACICU) OX=405416 GN=thiG PE=3 SV=1 ...
Keating TA, Marshall CG, Walsh CT (2000) Vibriobactin biosynthesis in Vibrio cholerae VibH is an amide synthase homologous to ... Brachmann AO, Joyce SA, Jenke-Kodama H, Schwär G, Clarke DJ, Bode HB (2007) A type II polyketide synthase is responsible for ...
In a continuing effort to unravel the structural basis for isoform-selective inhibition of nitric oxide synthase (NOS) by ... Rat neuronal NOS heme domain with D-lysine-D-nitroarginine amide bound. *DOI: 10.2210/pdb1RS6/pdb ... In a continuing effort to unravel the structural basis for isoform-selective inhibition of nitric oxide synthase (NOS) by ... The novel binding mode of N-alkyl-N-hydroxyguanidine to neuronal nitric oxide synthase provides mechanistic insights into NO ...
Nitric oxide synthase synonyms, Nitric oxide synthase pronunciation, Nitric oxide synthase translation, English dictionary ... definition of Nitric oxide synthase. n. See synthetase. n an enzyme that catalyses a process of synthesis ... Structural basis for dipeptide amide isoform-selective inhibition of neuronal nitric oxide synthase.. Role of the nitrergic ... synthase. (redirected from Nitric oxide synthase). Also found in: Medical, Acronyms, Encyclopedia, Wikipedia.. Related to ...
Structural role for a conserved region in the CTP synthetase glutamine amide transfer domain.. J. Bacteriol. 169 3023-8 1987 ... CTP synthase GATase domain (IPR033828). Short name: GATase1_CTP_Synthase Domain relationships *Glutamine amidotransferase ( ...
The hydroperoxy ligand deprotonates the amide which then closes the β-lactam ring by a nucleophilic attack at the thioaldehyde ... L-cysteinyl-D-valine synthase (ACV synthase). This allows for the binding of the substrate ACV to the deprotonated thiol group ... Isopenicillin N synthase (IPNS) is a non-heme iron-dependent enzyme belonging to the oxidoreductase family. This enzyme ... The active sites of most isopenicillin N synthases contain an iron ion. This enzyme is also called isopenicillin N synthetase. ...
  • A combined approach, comprising PCR screening and genome mining, was used to unravel the diversity and phylogeny of genes encoding 5-aminolevulinic acid synthases (ALASs, hemA gene products) in streptomycetes-related strains. (frontiersin.org)
  • A common variant in exon 7 of the endothelial constitutive nitric oxide synthase gene: Identification by single strand conformation polymorphism analysis. (thefreedictionary.com)
  • Disruption of the endothelial nitric oxide synthase gene affects ovulation, fertilization and early embryo survival in a knockout mouse model," Reproduction, vol. (thefreedictionary.com)
  • the sequence of the product of this gene, ainS , shows no similarity with members of the LuxI family of acyl-HSL synthases. (asm.org)
  • 4. The sorghum hybrid of claim 1, wherein said sorghum hybrid germplasm that confers resistance to inhibition by one or more acetolactate synthase herbicides comprises an acetolactate synthase gene, wherein said gene comprises one or more amino acid substitutions from the group consisting of Val 531 Ile and Trp 545 Leu. (freepatentsonline.com)
  • 8. The sorghum hybrid of claim 1, wherein said sorghum hybrid germplasm that confers resistance to inhibition by one or more acetolactate synthase herbicides comprises mutations in the acetolactate synthase gene as found in ATCC No. PTA-7999. (freepatentsonline.com)
  • 9. The sorghum hybrid of claim 8, wherein said acetolactate synthase gene as found in ATCC No. PTA-7999 is introduced into said sorghum hybrid by introgression. (freepatentsonline.com)
  • The system is a modified strain of E. coli engineered to express a biosynthetic pathway composed of four components: 1) biosynthetic genes from one species encoding enzymes for the assembly of a nonribosomal molecule, 2) biosynthetic genes from a second species encoding enzymes for the assembly of a different nonribosomal molecule, 3) a gene encoding an amide synthase, and 4) exogenously supplied building blocks. (mit.edu)
  • PZA is a prodrug of the pharmacologically active agent pyrazinoic acid (POA), which is formed upon hydrolysis of the amide by a mycobacterial pyrazinamidase encoded by the pncA gene ( 21 ). (asm.org)
  • The present invention relates to recombinant Gram-positive host cells containing a Gram-negative glycosaminoglycan synthase gene, and methods of producing glycosaminoglycans using such recombinant host cells. (freepatentsonline.com)
  • The putative aminotransferase (pAMT) and Pungent gene 1 (Pun1) proteins are believed to catalyze the second to last and the last steps in the pathway, respectively, making the Pun1 protein the putative capsaicin synthase. (biomedcentral.com)
  • In particular, the present invention provides for sorghum plants, plant tissues and plant seeds that contain altered acetolactate synthase (ALS) genes and proteins that are resistant to inhibition by herbicides that normally inhibit the activity of the ALS protein. (freepatentsonline.com)
  • 1. A sorghum hybrid wherein said sorghum hybrid germplasm confers resistance to inhibition by one or more acetolactate synthase herbicides at levels of said one or more herbicides that would normally inhibit the growth of a sorghum hybrid. (freepatentsonline.com)
  • 3. The sorghum hybrid of claim 1, wherein said resistance to inhibition by one or more acetolactate synthase herbicides is introduced into said sorghum hybrid germplasm by introgression. (freepatentsonline.com)
  • The results demonstrated significant inhibition of leukotriene-C 4 -synthase, leukotriene-A 4 -hydrolase and cyclooxygenase-2 (COX-2) activities by the EtOAc fraction of B. monnieri, suggesting a plausible mechanism of action for the management of asthma. (eurekaselect.com)
  • originating from activated nicotine amide dinucleotide phosphate oxidases, a dysfunctional mitochondrial respiratory chain, or uncoupled NO synthases. (ahajournals.org)
  • Recently, it has been shown that formation of the C 5 N unit and its attachment to core structures of antibiotics is directed by three enzymes: cyclizing 5-aminolevulinate synthase (cALAS encoded by a hemA homolog), amide synthase (AMS) and aminolevulinate-CoA ligase (ALL). (frontiersin.org)
  • In the presence of GTP, the k cat values for glutamine hydrolysis by the D107A and L109A enzymes were identical with that of wild-type CTP synthase. (biochemj.org)
  • BH4 is a cofactor for creating nitric oxide synthase (NOS) enzymes necessary for creating nitric oxide (Alp, 2004). (thefreedictionary.com)
  • We conclude that AinS is an acylhomoserine lactone synthase and that it represents a second family of such enzymes. (asm.org)
  • The LuxI family of acyl-HSL synthases constitutes a group of enzymes from different species. (asm.org)
  • The individual FAS component enzymes are ac(et)yltransferase (AC), malonyl/acetyl- or malonyl/palmitoyl-transacylase (AT, MPT), ketoacyl synthase (KS) ketoacyl reductase (KR), dehydratase (DH), enoyl reductase (ER), acyl carrier protein (ACP), and thioesterase (TE). (asm.org)
  • A comprehensive analysis has been made of the manner in which l-alanosine [l-2-amino-3-(N-nitroso, N-hydroxy) aminopropionic acid] interacts with the enzymes responsible for the metabolism of the dicarboxylic amino acids and their amides. (elsevier.com)
  • Discovery of N-(3-(1-methyl-1,2,3,6-tetrahydropyridin-4-yl)-1H-indol-6-yl) thiophene-2-carboximidamide as a selective inhibitor of human neuronal nitric oxide synthase (nNOS) for the treatment of pain. (nih.gov)
  • Discovery of cis-N-(1-(4-(methylamino)cyclohexyl)indolin-6-yl)thiophene-2-carboximidamide: a 1,6-disubstituted indoline derivative as a highly selective inhibitor of human neuronal nitric oxide synthase (nNOS) without any cardiovascular liabilities. (nih.gov)
  • Oligomycin has been recognized as a potent inhibitor of the mitochondrial ATP synthase since 1958 when it was reported by Henry Lardy et al. (pnas.org)
  • As a result, we identified a novel and highly selective Hs GSK3 inhibitor containing a 2-chloroaniline-substituted squaric acid amide pharmacophore that confers low nanomolar (IC 50 = 2.8 nM) and sub-micromolar potency against purified and cellular Hs GSK3. (rsc.org)
  • Abstract The metabolism, pharmacokinetics, tissue distribution, and excretion of L-N6-(1-iminoethyl)lysine-5-tetrazole-amide (L-NIL-TA), a selective inducible NO synthase (iNOS) inhibitor, were investigated in rats. (academicconcepts.net)
  • Rat liver showed the highest specific activities (4.4 +/- 0.3 and 4.5 +/- 0.5 nmol/min/mg protein) for the hydrolase and synthase, respectively. (nih.gov)
  • However, small intestine showed a relatively high synthase/hydrolase ratio of about 5.0 (1.0 +/- 0.1 and 0.2 +/- 0.1 nmol/min/mg protein). (nih.gov)
  • To gain insights about whether AinS and LuxM represent a second family of acylhomoserine lactone synthases, we have purified AinS as a maltose-binding protein (MBP) fusion protein. (asm.org)
  • Subunit-c of the ATP synthase is an integral membrane protein consisting of two helices, 1 and 2, which span the inner mitochondrial membrane ( Fig. 1 ). (pnas.org)
  • DNA-sequence analysis of LIP5 revealed that it encodes a protein homologous to lipoic acid synthase of E. coli . (plantphysiol.org)
  • This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag. (cusabio.com)
  • Additionally we are shipping Fatty Acid Synthase Kits (72) and Fatty Acid Synthase Proteins (14) and many more products for this protein. (antibodies-online.com)
  • Capsaicin and its analogs are called capsaicinoids, and the fundamental structure of most capsaicinoids is a branched-chain fatty acid amide of vanillylamine. (biomedcentral.com)
  • Ii produces feel- amide derivative of kanamydn inactivation by renal inducible no-synthase plays a critical role in terms of product quality even the acceleration following generation. (wisc.edu)
  • Amylin blocks the activation of glycogen synthase by insulin. (bioquote.com)
  • Glycogen synthase kinase 3 (GSK-3) can negatively regulate several aspects of insulin signaling, and elevated levels of GSK-3 have been reported in skeletal muscle from diabetic rodents and humans. (diabetesjournals.org)
  • These low molecular weight compounds activated glycogen synthase at ∼100 nmol/l in cultured CHO cells transfected with the insulin receptor and in primary hepatocytes isolated from Sprague-Dawley rats, and at 500 nmol/l in isolated type 1 skeletal muscle of both lean Zucker and ZDF rats. (diabetesjournals.org)
  • Glycogen synthase kinase 3 has evolutionarily conserved roles in cell signaling and metabolism and is a recognized drug target in neurological pathologies, most prominently bipolar disorder. (rsc.org)
  • We report the high-resolution (1.9 Å) crystal structure of oligomycin bound to the subunit c 10 ring of the yeast mitochondrial ATP synthase. (pnas.org)
  • Here we report the oligomycin-binding site on subunit-c of the F o portion of the ATP synthase. (pnas.org)
  • Subunit-c assembles as a homomeric ring consisting of 10 subunits in the yeast ATP synthase and eight subunits in the bovine ATP synthase ( 5 , 6 ). (pnas.org)
  • Sucrose phosphate synthase (SPS) catalyzes the transfer of a glycosyl group from an activated donor sugar, such as uridine diphosphate glucose (UDP-Glc), to a saccharide acceptor d -fructose 6-phosphate (F6P), resulting in the formation of UDP and d -sucrose-6′-phosphate (S6P). (plantcell.org)
  • Lipoic acid is covalently bound to these proteins via an amide linkage to the ε-amino group of specific Lys residues ( Reed and Hackert, 1966 ). (plantphysiol.org)
  • The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. (nih.gov)
  • 1 ). Studies in the 1960s from the laboratory of Efraim Racker demonstrated that the mitochondrial ATP synthase can be separated into two parts, coupling factor 1, or F 1 , which contains the catalytic site for ATP synthesis, and coupling factor o, or F o , which is able to confer sensitivity to oligomycin ( 2 ⇓ - 4 ). (pnas.org)
  • Despite more than 50 y of studies on mitochondrial F 1 F o ATP synthase, the binding site of oligomycin on F o has been elusive. (pnas.org)
  • ET-1 increases superoxide production in human coronary artery bypass grafts via a receptor-driven mechanism involving, the largest contributor of superoxide in the vascular wall, nicotine amide dinucleotide phosphate (NADPH) oxidase. (avhandlingar.se)
  • The rapid conversion of PR toxin to the other derivatives such as PR imine, PR amide, and PR acid, based on conditions available reflects their unstability and degradative aspects. (frontiersin.org)
  • Folic acid prevents nitroglycerin-induced nitric oxide synthase dysfunction and nitrate tolerance: a human in vivo study. (thefreedictionary.com)
  • An analog of pyrazinamide (PZA), 5-chloropyrazinamide (5-Cl-PZA), has previously been shown to inhibit mycobacterial fatty acid synthase I (FASI). (asm.org)
  • The observations that 5-Cl-PZA inhibits fatty acid synthase I (FASI) in M. smegmatis and that both 5-Cl PZA and PZA inhibit M. tuberculosis FASI (albeit with the proviso that PZA requires acidic conditions) ( 32 ) suggest that FASI is a potential antituberculosis drug target. (asm.org)
  • 2. The recombinant host cell of claim 1, wherein the purified nucleic acid segment encodes the Pasteurella multocida chondroitin synthase of SEQ ID NO:2. (freepatentsonline.com)
  • 9. The method of claim 8 wherein, in the step of providing a purified nucleic acid segment encoding an enzymatically active Pasteurella chondroitin synthase, the purified nucleic acid segment encodes the Pasteurella multocida chondroitin synthase of SEQ ID NO:2. (freepatentsonline.com)
  • We report here the isolation and characterization of LIP1 cDNA for lipoic acid synthase of Arabidopsis. (plantphysiol.org)
  • This cDNA was shown to code for Arabidopsis lipoic acid synthase by its ability to complement a lipA mutant of E. coli defective in lipoic acid synthase. (plantphysiol.org)
  • lipA encodes a lipoic acid synthase that is required for an insertion of the first sulfur atom into the octanoate backbone ( Reed and Cronan, 1993 ). (plantphysiol.org)
  • The present review focuses on microbial type I fatty acid synthases (FASs), demonstrating their structural and functional diversity. (asm.org)
  • According to the textbook reaction mechanism, fatty acid synthase is an "iterative" multienzyme, performing several successive cycles of a distinct reaction sequence in combination with a specific initiation and termination reaction at the beginning and end of the overall process, respectively. (asm.org)
  • On www.antibodies-online.com are 215 Fatty Acid Synthase (FASN) Antibodies from 27 different suppliers available. (antibodies-online.com)
  • A total of 309 Fatty Acid Synthase products are currently listed. (antibodies-online.com)
  • Oliveras-Ferraros, Vazquez-Martin, Fernández-Real, Menendez: AMPK-sensed cellular energy state regulates the release of extracellular Fatty Acid Synthase. (antibodies-online.com)
  • Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate. (nih.gov)
  • The extracts and fractions were tested for their effects on leukotriene C 4 synthase, leukotriene A4hydrolase and cyclooxygenase-2 in HL-60 cells. (eurekaselect.com)
  • Results: Bacopa monnieri extracts and partitions inhibited leukotriene-C 4 -synthase, leukotriene-A 4 -hydrolase and/or cyclooxygenase- 2 (COX-2) activities in cultured HL-60 cells. (eurekaselect.com)
  • The two molecule intermediates and one building block molecule are condensed via the aforementioned amide synthase to generate one hybrid nonribosomal molecule. (mit.edu)
  • Some α-Proteobacteria, fungi, and animals form ALA through condensation of glycine and succinyl-CoA by hemA -encoded aminolevulinate synthase in so called C4 or Shemin pathway. (frontiersin.org)
  • In the MEP pathway, 4-diphosphocytidyl-2- C -methyl- d -erythritol is formed from 2- C -methyl- d -erythritol 4-phosphate (MEP) and CTP in a reaction catalyzed by a 4-diphosphocytidyl-2- C -methyl- d -erythritol synthase (IspD). (asm.org)
  • First-in-class, dual-action, 3,5-disubstituted indole derivatives having human nitric oxide synthase (nNOS) and norepinephrine reuptake inhibitory (NERI) activity for the treatment of neuropathic pain. (nih.gov)
  • The anandamide, or arachidonoiletanolammide (AEA), is part of a new class of lipid mediators, acting predomi-nantly olocrina and paracrine, collectively known as en-docannabinoids. (alliedacademies.org)