Using genomic information to investigate the function of ThiI, an enzyme shared between thiamin and 4-thiouridine biosynthesis. (1/66)

The gene thiI encodes a protein (ThiI) that plays a role in the transfer of sulfur from cysteine to both thiamin and 4-thiouridine, but the reaction catalyzed by ThiI remains undetermined. Based upon sequence alignments, ThiI shares a unique "P-loop" motif with the PPi synthetase family, four enzymes that catalyze adenylation and subsequent substitution of carbonyl oxygens. To test whether or not this motif is critical for ThiI function, the Asp in the motif was converted to Ala (D189A), and a screen for in vivo 4-thiouridine production revealed the altered enzyme to be inactive. Further scrutiny of sequence data and the crystal structures of two members of the PPi synthetase family implicated Lys321 in the proposed adenylation function of ThiI, and the critical nature of Lys321 has been demonstrated by site-directed mutagenesis and genetic screening. Our results, then, indicate that ThiI catalyzes the adenylation of a substrate at the expense of ATP, a narrowing of possible reactions that provides a strong new basis for deducing the early steps in the transfer of sulfur from cysteine to both thiamin and 4-thiouridine.  (+info)

Identification of the novobiocin biosynthetic gene cluster of Streptomyces spheroides NCIB 11891. (2/66)

The novobiocin biosynthetic gene cluster from Streptomyces spheroides NCIB 11891 was cloned by using homologous deoxynucleoside diphosphate (dNDP)-glucose 4,6-dehydratase gene fragments as probes. Double-stranded sequencing of 25.6 kb revealed the presence of 23 putative open reading frames (ORFs), including the gene for novobiocin resistance, gyrB(r), and at least 11 further ORFs to which a possible role in novobiocin biosynthesis could be assigned. An insertional inactivation experiment with a dNDP-glucose 4, 6-dehydratase fragment resulted in abolishment of novobiocin production, since biosynthesis of the deoxysugar moiety of novobiocin was blocked. Heterologous expression of a key enzyme of novobiocin biosynthesis, i.e., novobiocic acid synthetase, in Streptomyces lividans TK24 further confirmed the involvement of the analyzed genes in the biosynthesis of the antibiotic.  (+info)

Cloning, overexpression, and purification of novobiocic acid synthetase from Streptomyces spheroides NCIMB 11891. (3/66)

Novobiocic acid synthetase, a key enzyme in the biosynthesis of the antibiotic novobiocin, was cloned from the novobiocin producer Streptomyces spheroides NCIMB 11891. The enzyme is encoded by the gene novL, which codes for a protein of 527 amino acids with a calculated mass of 56,885 Da. The protein was overexpressed as a His(6) fusion protein in Escherichia coli and purified to apparent homogeneity by affinity chromatography and gel chromatography. The purified enzyme catalyzed the formation of an amide bond between 3-dimethylallyl-4-hydroxybenzoic acid (ring A of novobiocin) and 3-amino-4,7-dihydroxy-8-methyl coumarin (ring B of novobiocin) in an ATP-dependent reaction. NovL shows homology to the superfamily of adenylate-forming enzymes, and indeed the formation of an acyl adenylate from ring A and ATP was demonstrated by an ATP-PP(i) exchange assay. The purified enzyme exhibited both activation and transferase activity, i.e. it catalyzed both the activation of ring A as acyl adenylate and the subsequent transfer of the acyl group to the amino group of ring B. It is active as a monomer as determined by gel filtration chromatography. The reaction was specific for ATP as nucleotide triphosphate and dependent on the presence of Mg(2+) or Mn(2+). Apparent K(m) values for ring A and ring B were determined as 19 and 131 micrometer respectively. Of several analogues of ring A, only 3-geranyl-4-hydroxybenzoate and to a lesser extent 3-methyl-4-aminobenzoate were accepted as substrates.  (+info)

Characterization of recombinant glutathionylspermidine synthetase/amidase from Crithidia fasciculata. (4/66)

Trypanothione [N1,N8-bis(glutathionyl)spermidine] is a unique metabolite found only in trypanosomatids, where it subsumes many of the functions of GSH in other organisms. In Crithidia fasciculata, two distinct ATP-dependent ligases, glutathionylspermidine synthetase (GspS; EC 6.3.1.8) and trypanothione synthetase (TryS; EC 6.3.1.9), are involved in the synthesis of trypanothione from GSH and spermidine. Both enzymes have been cloned previously, but expression in Escherichia coli produced insoluble and inactive protein. Here we report on the successful expression of soluble (His)6-tagged C. fasciculata GspS in E. coli. Following purification using nickel-chelating affinity chromatography, the tag sequence was removed and the enzyme purified to homogeneity by anion-exchange chromatography. The kinetic parameters of the recombinant enzyme have been determined using a coupled enzyme assay and also by HPLC analysis of end-product formation. Under optimal conditions (0.1 M K+-Hepes, pH 7.3) GspS has synthetase activity with apparent K(m) values for GSH, spermidine and MgATP of 242, 59 and 114 microM respectively, and a k(cat) of 15.5 s(-1). Glutathionylspermidine is formed as end product and the enzyme lacks TryS activity. Like E. coli GspS, the recombinant enzyme also possesses amidase activity (EC 3.5.1.78), hydrolysing glutathionylspermidine to GSH and spermidine with a k(cat) of 0.38 s(-1) and a K(m) of 500 microM. GspS can also hydrolyse trypanothione at about 1.5% of the rate with glutathionylspermidine. A single amino acid mutation (Cys-79-->Ala) is shown to ablate the amidase activity without affecting the synthetase activity.  (+info)

A single enzyme catalyses formation of Trypanothione from glutathione and spermidine in Trypanosoma cruzi. (5/66)

Protozoa of the order Kinetoplastida differ from other organisms in their ability to conjugate glutathione (l-gamma-glutamyl-cysteinyl-glycine) and spermidine to form trypanothione [N(1),N(8)-bis(glutathionyl)spermidine], a metabolite involved in defense against chemical and oxidant stress and other biosynthetic functions. In Crithidia fasciculata, trypanothione is synthesized from GSH and spermidine via the intermediate glutathionylspermidine in two distinct ATP-dependent reactions catalyzed by glutathionylspermidine synthetase (GspS; EC ) and trypanothione synthetase (TryS; EC ), respectively. Here we have cloned a single copy gene (TcTryS) from Trypanosoma cruzi encoding a protein with 61% sequence identity with CfTryS but only 31% with CfGspS. Saccharomyces cerevisiae transformed with TcTryS were able to synthesize glutathionylspermidine and trypanothione, suggesting that this enzyme is able to catalyze both biosynthetic steps, unlike CfTryS. When cultures were supplemented with aminopropylcadaverine, yeast transformants contained glutathionylaminopropylcadaverine and homotrypanothione [N(1),N(9)-bis(glutathionyl)aminopropylcadaverine], metabolites that have been previously identified in T. cruzi, but not in C. fasciculata. Kinetic studies on recombinant TcTryS purified from Escherichia coli revealed that the enzyme displays high-substrate inhibition with glutathione (K(m) and K(i) of 0.57 and 1.2 mm, respectively, and k(cat) of 3.4 s(-1)), but obeys Michaelis-Menten kinetics with spermidine, aminopropylcadaverine, glutathionylspermidine, and MgATP as variable substrate. The recombinant enzyme possesses weak amidase activity and can hydrolyze trypanothione, homotrypanothione, or glutathionylspermidine to glutathione and the corresponding polyamine.  (+info)

Stable ammonia-specific NAD synthetase from Bacillus stearothermophilus: purification, characterization, gene cloning, and applications. (6/66)

Bacillus stearothermophilus H-804 isolated from a hot spring in Beppu, Japan, produced an ammonia-specific NAD synthetase (EC 6.3.1.5). The enzyme specifically used NH3 as an amide donor for the synthesis of NAD as it formed AMP and pyrophosphate from deamide-NAD and ATP. None of the l-amino acids tested, such as l-asparagine or l-glutamine, or other amino compounds such as urea, uric acid, or creatinine was used instead of NH3. Mg2+ was needed for the activity, and the maximum enzyme activity was obtained with 3 mM MgCl2. The molecular mass of the native enzyme was 50 kDa by gel filtration, and SDS-PAGE showed a single protein band at the molecular mass of 25 kDa. The optimum pH and temperature for the activity were from 9.0 to 10.0 and 60 degrees C, respectively. The enzyme was stable at a pH range of 7.5 to 9.0 and up to 60 degrees C. The Km for NH3, ATP, and deamide-NAD were 0.91, 0.052, and 0.028 mM, respectively. The gene encoding the enzyme consisted of an open reading frame of 738 bp and encoded a protein of 246 amino acid residues. The deduced amino acid sequence of the gene had about 32% homology to those of Escherichia coli and Bacillus subtilis NAD synthetases. We caused the NAD synthetase gene to be expressed in E. coli at a high level; the enzyme activity (per liter of medium) produced by the recombinant E. coli was 180-fold that of B. stearothermophilus H-804. The specific assay of ammonia and ATP (up to 25 microM) with this stable NAD synthetase was possible.  (+info)

Molecular identification of human glutamine- and ammonia-dependent NAD synthetases. Carbon-nitrogen hydrolase domain confers glutamine dependency. (7/66)

NAD synthetase catalyzes the final step in the biosynthesis of NAD. In the present study, we obtained cDNAs for two types of human NAD synthetase (referred as NADsyn1 and NADsyn2). Structural analysis revealed in both NADsyn1 and NADsyn2 a domain required for NAD synthesis from ammonia and in only NADsyn1 an additional carbon-nitrogen hydrolase domain shared with enzymes of the nitrilase family that cleave nitriles as well as amides to produce the corresponding acids and ammonia. Consistent with the domain structures, biochemical assays indicated (i) that both NADsyn1 and NADsyn2 have NAD synthetase activity, (ii) that NADsyn1 uses glutamine as well as ammonia as an amide donor, whereas NADsyn2 catalyzes only ammonia-dependent NAD synthesis, and (iii) that mutant NADsyn1 in which Cys-175 corresponding to the catalytic cysteine residue in nitrilases was replaced with Ser does not use glutamine. Kinetic studies suggested that glutamine and ammonia serve as physiological amide donors for NADsyn1 and NADsyn2, respectively. Both synthetases exerted catalytic activity in a multimeric form. In the mouse, NADsyn1 was seen to be abundantly expressed in the small intestine, liver, kidney, and testis but very weakly in the skeletal muscle and heart. In contrast, expression of NADsyn2 was observed in all tissues tested. Therefore, we conclude that humans have two types of NAD synthetase exhibiting different amide donor specificity and tissue distributions. The ammonia-dependent synthetase has not been found in eucaryotes until this study. Our results also indicate that the carbon-nitrogen hydrolase domain is the functional domain of NAD synthetase to make use of glutamine as an amide donor in NAD synthesis. Thus, glutamine-dependent NAD synthetase may be classified as a possible glutamine amidase in the nitrilase family. Our molecular identification of NAD synthetases may prove useful to learn more of mechanisms regulating cellular NAD metabolism.  (+info)

Eukaryotic NAD+ synthetase Qns1 contains an essential, obligate intramolecular thiol glutamine amidotransferase domain related to nitrilase. (8/66)

NAD+ is an essential co-enzyme for redox reactions and is consumed in lysine deacetylation and poly(ADP-ribosyl)ation. NAD+ synthetase catalyzes the final step in NAD+ synthesis in the well characterized de novo, salvage, and import pathways. It has been long known that eukaryotic NAD+ synthetases use glutamine to amidate nicotinic acid adenine dinucleotide while many purified prokaryotic NAD+ synthetases are ammonia-dependent. Earlier, we discovered that glutamine-dependent NAD+ synthetases contain N-terminal domains that are members of the nitrilase superfamily and hypothesized that these domains function as glutamine amidotransferases for the associated synthetases. Here we show yeast glutamine-dependent NAD+ synthetase Qns1 requires both the nitrilase-related active-site residues and the NAD+ synthetase active-site residues for function in vivo. Despite failure to complement the lethal phenotype of qns1 disruption, the former mutants retain ammonia-dependent NAD+ synthetase activity in vitro, whereas the latter mutants retain basal glutaminase activity. Moreover, the two classes of mutants fail to trans-complement despite forming a stable heteromultimer in vivo. These data indicate that the nitrilase-related domain in Qns1 is the fourth independently evolved glutamine amidotransferase domain to have been identified in nature and that glutamine-dependence is an obligate phenomenon involving intramolecular transfer of ammonia over a predicted distance of 46 A from one active site to another within Qns1 monomers.  (+info)

*Vibriobactin

VibH Is an Amide Synthase Homologous to Nonribosomal Peptide Synthetase Condensation Domains". Biochemistry. 39: 15514. ...

*4-methyleneglutamate-ammonia ligase

... amide synthases). The systematic name of this enzyme class is 4-methylene-L-glutamate:ammonia ligase (AMP-forming). This enzyme ... Winter HC, Su TZ, Dekker EE (1983). "4-methyleneglutamine synthetase: a new amide synthetase present in germinating peanuts". ...

*Aspartate-ammonia ligase

... amide synthases). The systematic name of this enzyme class is L-aspartate:ammonia ligase (AMP-forming). Other names in common ...

*Trypanothione synthase

... amide synthases). The systematic name of this enzyme class is glutathionylspermidine:glutathione ligase (ADP-forming). The ... Targeting trypanothione synthase could be a novel way of preventing and curing these diseases through disruption of the ... Evidence for this regulation is that the residues which allow the synthase domain to block the amidase active site are highly ... The main function of trypanothione synthase is to use the free energy generated from ATP hydrolysis to conjugate glutathione ...

*Glutathionylspermidine synthase

... amide synthases). The systematic name of this enzyme class is gamma-L-glutamyl-L-cysteinyl-glycine:spermidine ligase (ADP- ... In enzymology, a glutathionylspermidine synthase (EC 6.3.1.8) is an enzyme that catalyzes the chemical reaction glutathione + ...

*D-aspartate ligase

... amide synthases). The systematic name of this enzyme class is D-aspartate:[beta-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L- ...

*Glutamate-putrescine ligase

... amide synthases). The systematic name of this enzyme class is L-glutamate:putrescine ligase (ADP-forming). Other names in ...

*NAD+ synthase

... amide synthases). The systematic name of this enzyme class is deamido-NAD+:ammonia ligase (AMP-forming). Other names in common ... In enzymology, a NAD+ synthase (EC 6.3.1.5) is an enzyme that catalyzes the chemical reaction ATP + deamido-NAD+ + NH3 ⇌ {\ ... use include NAD+ synthetase, NAD+ synthase, nicotinamide adenine dinucleotide synthetase, and diphosphopyridine nucleotide ...

*Rifamycin

In all, rifA-E assemble a linear undecaketide and are followed by rifF, which encodes an amide synthase and causes the ... "RifH" encodes aminoDAHP synthase that catalyzes the condensation between 1-deoxy-1-imino-d-erythrose 4-phosphate and ... RifA through rifE encode a type I polyketide synthase module, with the loading module being a non-ribosomal peptide synthetase ... Other types of genes seem to perform post-synthase modifications of the original polyketide. Lepetit introduced Rifampicin, an ...

*Peptidylglycine monooxygenase

... peptide-alpha-amide synthetase, synthase, peptide alpha-amide, peptide alpha-amidating enzyme, peptide alpha-amide synthase, ... Bradbury AF, Finnie MD, Smyth DG (1982). "Mechanism of C-terminal amide formation by pituitary enzymes". Nature. 298 (5875): ...

*List of MeSH codes (D08)

... adenylosuccinate synthase MeSH D08.811.464.259.200 --- amide synthases MeSH D08.811.464.259.200.200 --- aspartate-ammonia ... riboflavin synthase MeSH D08.811.913.225.825 --- spermidine synthase MeSH D08.811.913.225.912 --- spermine synthase MeSH ... carbamoyl-phosphate synthase (ammonia) MeSH D08.811.464.259.400 --- carbon-nitrogen ligases with glutamine as amide-n-donor ... nitric oxide synthase type i MeSH D08.811.682.664.500.772.500 --- nitric oxide synthase type ii MeSH D08.811.682.664.500.772. ...

*Nitrogen assimilation

Glutamate synthase (Fd-GOGAT and NADH-GOGAT) transfer the amide group onto an 2-oxoglutarate molecule producing two glutamates ... Lea, P. J.; Miflin, B. J. (2003). "Glutamate synthase and the synthesis of glutamate in plants". Plant Physiology and ... In the chloroplasts, glutamine synthetase incorporates this ammonia as the amide group of glutamine using glutamate as a ... Ammonia (both absorbed and synthesized) is incorporated into amino acids via the glutamine synthetase-glutamate synthase (GS- ...

*Pyruvate synthase

It is composed of a nitrothiazole-ring and a salicylic acid moiety which are linked together by an amide bond ... NTZ is ... In enzymology, a pyruvate synthase (EC 1.2.7.1) is an enzyme that catalyzes the interconversion of pyruvate and acetyl-CoA. It ... Gehring U, Arnon DI (1972). "Purification and properties of -ketoglutarate synthase from a photosynthetic bacterium". J. Biol. ...

*CO-methylating acetyl-CoA synthase

Nid is coordinated to two cysteine molecules and two backbone amide compounds, and is in a square-planar coordination. The ... Acetyl-CoA Synthase (ACS), not to be confused with Acetyl-CoA Synthetase or Acetate-CoA Ligase (ADP forming), is a Nickel ... Acetyl-CoA Synthase (ACS) and carbon monoxide dehydrogenase (CODH) are integral enzymes in this one pathway and can perform ... The recommended name for this enzyme is CO-methylating acetyl-CoA synthase. In nature, there are six different pathways where ...

*Isopenicillin N synthase

The hydroperoxy ligand deprotonates the amide which then closes the β-lactam ring by a nucleophilic attack at the thioaldehyde ... L-cysteinyl-D-valine synthase (ACV synthase). This allows for the binding of the substrate ACV to the deprotonated thiol group ... Isopenicillin N synthase (IPNS) is a non-heme iron-dependent enzyme belonging to the oxidoreductase family. This enzyme ... The active sites of most isopenicillin N synthases contain an iron ion. This enzyme is also called isopenicillin N synthetase. ...

*Glutamate synthase (NADPH)

... synthase, glutamate synthase (NADPH), glutamate synthetase (NADP), glutamine amide-2-oxoglutarate aminotransferase ( ... Glutamate synthase (NADH) Glutamate synthase (ferredoxin) Temple SJ, Vance CP, Gantt JS (1998). "Glutamate synthase and ... NADPH-dependent glutamate synthase, NADPH-glutamate synthase, and NADPH-linked glutamate synthase. As of late 2007, only one ... In enzymology, a glutamate synthase (NADPH) (EC 1.4.1.13) is an enzyme that catalyzes the chemical reaction L-glutamine + 2- ...

*Coenzyme F420-0:L-glutamate ligase

"Structure of an amide bond forming F420:γ-glutamyl ligase from Archaeoglobus fulgidus - a member of a new family of non- ... ribosomal peptide synthases". J. Mol. Biol. 372: 456-469. doi:10.1016/j.jmb.2007.06.063. PMC 2678844 . PMID 17669425. Coenzyme ...

*Hementerin

It has no degrading action on amide, plasminogen and casein. In addition, hementerin inhibits platelet aggregation induced by ... Inhibition is probably achieved by enhanced nitric oxide synthase activity. Calcium is a cofactor (ligand) in this activity. ...

*Dazoxiben

... is an orally active thromboxane synthase inhibitor. It has shown a significant clinical improvement in patients with ... Hydrolysis of the amide function completes the synthesis of dazoxiben. Belch, J. J.; Cormie, J.; Newman, P.; McLaren, M.; ...

*Nickel compounds

Nickel amide, Ni(NH2)2 is a deep red compound that contains Ni6 clusters surrounded by 12 NH2 groups. Nickel amide also forms a ... CO-methylating acetyl-CoA synthase contains two active nickel atoms, one is held in a square planar coordination by two ... Other homoleptic nickel amides derived by substituting the hydrogen atoms are Ni[N(C6H5)2]2 (diphenyl) and boryl amides Ni[ ... Various double amides containing nickel clusters have been made using liquid ammonia as a solvent. Substances made include red ...

*N-methyl-2-oxoglutaramate hydrolase

This enzyme is also called 5-hydroxy-N-methylpyroglutamate synthase. Hersh LB (1970). "5-hydroxy-N-methylpyroglutamate ... specifically in linear amides. The systematic name of this enzyme class is N-methyl-2-oxoglutaramate methylamidohydrolase. ...

*Integral monotopic protein

... lanosterol synthase and squalene-hopene cyclase microsomal prostaglandin E synthase carnitine O-palmitoyltransferase 2 There ... are also structures of integral monotopic domains of transmembrane proteins: monoamine oxidases A and B fatty acid amide ... "A Computational Protocol for the Integration of the Monotopic Protein Prostaglandin H2 Synthase into a Phospholipid Bilayer". ...

*Palmitoylethanolamide

PEA, as well as its analogue adelmidrol (di-amide derivative of azelaic acid), can both down-regulate mast cells. PEA reduces ... the expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) and prevents IkB-alpha degradation and ... Palmitoylethanolamide (PEA) is an endogenous fatty acid amide, belonging to the class of nuclear factor agonists. PEA has been ... Calignano a, L. R. G. (2001). "Antinociceptive activity of the endogenous fatty acid amide, palmitylethanolamide". Eur J ...

*Serine hydrolase

... including fatty acid synthase and the acyl-CoA thioesterases Some phospholipases, including phospholipase A2 and platelet ... including fatty acid amide hydrolase Some peptidases, including dipeptidyl peptidase 4, fibroblast activation protein, and ... prolylendopeptidase Enzyme List of enzymes Ester Amide Thioester Activity based proteomics Alpha/beta hydrolase fold Simon GM, ...

*Lipoic acid

As a cofactor, RLA is covalently attached by an amide bond to a terminal lysine residue of the enzyme's lipoyl domains. One of ... Two hydrogens of octanoate are replaced with sulfur groups via a radical SAM mechanism, by lipoyl synthase As a result, lipoic ... The octanoate is transferred as a thioester of acyl carrier protein from fatty acid biosynthesis to an amide of the lipoyl ...

*Index of biochemistry articles

... amide - amine - amino - amino acid - amino acid receptor - amino acid sequence - amino acid sequence homology - aminobutyric ... ATP synthase - ATPase - atrial natriuretic factor - atrial natriuretic factor receptor - Avogadro's number - axon - B cell - ...
La glutationilspermidina sintasi è un enzima numero EC 6.3.1.8 appartenente alla classe delle ligasi, che catalizza la seguente reazione: glutatione + spermidina + ATP ⇄ glutationilspermidina + ADP + fosfato ^ (EN) 6.3.1.8, in ExplorEnz - The Enzyme Database, IUBMB. (EN) Smith K, Nadeau K, Bradley M, Walsh C, Fairlamb AH, Purification of glutathionylspermidine and trypanothione synthetases from Crithidia fasciculata, in Protein. Sci., vol. 1, nº 7, 1992, pp. 874-83, DOI:10.1002/pro.5560010705, PMC 2142158, PMID 1304372. (EN) Bollinger JM Jr, Kwon DS, Huisman GW, Kolter R, Walsh CT, Glutathionylspermidine metabolism in Escherichia coli. Purification, cloning, overproduction, and characterization of a bifunctional glutathionylspermidine synthetase/amidase, in J. Biol. Chem., vol. 270, nº 23, 1995, pp. 14031-41, DOI:10.1074/jbc.270.23.14031, PMID 7775463. Glutationilspermidina ...
trypanothione synthetase: catalyzes the synthesis of N(1)- and N(8)-glutathionylspermidine and trypanothione from ATP/Mg, glutathione, and spermidine; member of ATP-dependent class of ligases forming an amide linkage
In enzymology, a NAD+ synthase (EC 6.3.1.5) is an enzyme that catalyzes the chemical reaction:ATP + deamido-NAD+ + NH3↔ AMP + diphosphate + NAD+. The 3 substrates of th
... MEANING: Americana short for gonads....She gave me a good nad rub after we had sex...suppose it is like the word ass to arse difference between Ameri-Brit with the r the vocal accentual difference. Add slang to CoolSlang!
Methodology provided: The group of Luise Krauth-Siegel is highly experienced in the functional and kinetic characterization of thiol redox proteins. They have established methods for the sensitive and quantitative determination of low molecular mass thiols as well as for the large scale production of glutathionylspermidine and trypanothione. The tools for inducible RNA-interference and gene deletion in T. brucei are available. Methods for the quantitative proteome LC/MS analysis are currently established (in collaboration with Thomas Ruppert, ZMBH ...
QNS is the online home for Queens providing the most comprehensive source of award-winning local news, events, classifieds and a social platform to connect and inform members of each neighborhood ...
QNS is the online home for Queens providing the most comprehensive source of award-winning local news, events, classifieds and a social platform to connect and inform members of each neighborhood ...
JJ applies a boston crab to Kidman. Charles Robinson asks Kidman if he quits. ... Kidman is fighting the hold. ... (AHHHH!) ... ... (AHHHH!) Double J breaks the hold. Kidman gets up. Kidman punches Jeff Jarrett in the head. Double J tackles and begins punching Kidman. Kidman gets back to his feet. Kidman delivers a kick to the head of Jeff Jarrett. Jeff Jarrett is up again. JJ trys for a Vertical Suplex but is not strong enough to lift Kidman.JJ goes for a figure four. Charles Robinson is checking for a tap out. ... (AHHHH!) ... JJ tightens the hold. Kidman escapes. Double J trys for a Spine Buster but is not strong enough to lift Kidman.Double J applies a boston crab to Kidman. The referee is checking the situation. ... ... ... Kidman is fighting the hold. Kidman escapes. JJ and Kidman go to the floor Charles Robinson starts the count (.1) (..2) Jeff Jarrett takes Kidman down with a knee. Kidman moves back to his feet. (...3) Kidman tackles JJ. Now Kidman standing. (....4) Kidman fist drops JJ ...
6.3.1 Acid-D-ammonia (or amine) ligases (amide synthases ...
I was wondering if you guys have any experience with a very low dose SERM along with enanthate or cypionate to elevate LH instead of using HCG.. (some docs wont Rx HCG) but are fine with SERM.. Ohhhh ya .. while on T , I hated having soft nads. I am currently trying to get back to baseline with nothing.. and see where I stand. How and feel and how my bloods look.. Its been 8months since my last Clomid..
23. Cactus vs. Sabu (this is their 2nd match, Sabu breaks 3 ribs doing a moonsault and landing on the guardrail, Sabu breaks a bottle over Cactuss head but it takes 5 trys to break it, you can hear the thud with each blow, sick match) ...
Has anyone out there had prolems with Hotmail or MSN e-mail over the past 2 weeks? It is driving me nuts! I cant send attachments witout getting Server Busy or other errors. I get that on normal messages as well. When I do get a message out (1 ot of 10 trys) it sends more than one copy. When I get an attachment it thinks it has a virus. Ugggh. I know other people are geting the same problem but I havent heard when itll be fixed or what the problem is. Ive sent a couple messages to Hotmail and the one canned response I got was that they were aware of a problem and were working on it. How do they plan on keeping customers over this? Its been at least 2 weeks now ...
23. Cactus vs. Sabu (this is their 2nd match, Sabu breaks 3 ribs doing a moonsault and landing on the guardrail, Sabu breaks a bottle over Cactuss head but it takes 5 trys to break it, you can hear the thud with each blow, sick match ...
Alpha-oligopeptide / Glutamine or derivatives / N-acyl-alpha amino acid or derivatives / Alpha-amino acid amide / Cysteine or derivatives / Alpha-amino acid / D-alpha-amino acid / N-substituted-alpha-amino acid / Alpha-amino acid or derivatives / Fatty acylFatty acid / Dicarboxylic acid or derivatives / N-acyl-amine / Fatty amide / Organic disulfide / Dialkyldisulfide / Carboxamide group / Secondary carboxylic acid amide / Amino acid or derivatives / Amino acid / Sulfenyl compound / Carboxylic acid / Secondary aliphatic amine / Secondary amine / Primary amine / Organopnictogen compound / Organic oxygen compound / Organic nitrogen compound / Carbonyl group / Hydrocarbon derivative / Amine / Organic oxide / Primary aliphatic amine / Organosulfur compound / Organooxygen compound / Organonitrogen compound / Aliphatic acyclic compound ...
Thirteen genomic regions showing strong geographical variation are listed in Table 1, and Supplementary Fig. 7 shows the way in which their allele frequencies vary geographically. The predominant pattern is variation along a NW/SE axis. The most likely cause for these marked geographical differences is natural selection, most plausibly in populations ancestral to those now in the UK. Variation due to selection has previously been implicated at LCT (lactase) and major histocompatibility complex (MHC), and within-UK differentiation at 4p14 has been found independently, but others seem to be new findings. All but three of the regions contain known genes. Aside from evolutionary interest, genes showing evidence of natural selection are particularly interesting for the biology of traits such as infectious diseases; possible targets for selection include NADSYN1 (NAD synthetase 1) at 11q13, which could have a role in prevention of pellagra, as well as TLR1 (toll-like receptor 1) at 4p14, for which a ...
Looking for the definition of QNS? Find out what is the full meaning of QNS on Abbreviations.com! Questions is one option -- get in to view more @ The Webs largest and most authoritative acronyms and abbreviations resource.
Retinoid found in: NeoStrata Bionic Face Cream 40g, NeoStrata Renewal Cream 12 PHA 30g, Nads Facial Wax Strips 20 Strips, Nads Body Wax Strips For..
Background: Experimental evidence has demonstrated an anti-neoplastic role for vitamin D in the colon and higher circulating 25-hydroxyvitamin D (25[OH]D) levels are consistently associated with a lower risk of colorectal cancer (CRC). Genome-wide association studies have identified loci associated with levels of circulating 25(OH)D. The identified SNPs from four gene regions, collectively explain approximately 5% of the variance in circulating 25(OH)D. Methods: We investigated whether six polymorphisms in GC, CYP2R1, CYP24A1 and DHCR7/NADSYN1, genes previously shown to be associated with circulating 25(OH)D levels, were associated with CRC risk in 10,061 cases and 12,768 controls drawn from 13 studies included in the Genetics and Epidemiology of Colorectal Cancer Consortium (GECCO) and Colon Cancer Family Registry (CCFR). We performed a meta-analysis of crude and multivariate-adjusted logistic regression models to calculate odds ratios and associated confidence intervals for SNPs individually, ...
In the four months prior to April 2015, the Commission had declined two clearance applications, one being the acquisition of the Durex lube business…
Originally Posted by dreamstang Not to speak of your nads but I bet they could handle it. I would love to have a 10 sec car to. But I dont think that
Short and to the point My nads are drawing up in their satchel quite tight....kinda of like when it gets cold....only its NOT cold at all i live in
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when you do the climb with spider man go to the window all the way to the right and climb above it and drop and it will take you to a secret room it might take a couple of trys to. ...
brandy got ran over about 4 days ago,,, she is walking with a lamp,,, eating and drinknihg,,, but she hasnt peeor poop. she trys but just like a dribble pee an...
when you do the climb with spider man go to the window all the way to the right and climb above it and drop and it will take you to a secret room it might take a couple of trys to. ...
you see i had a unstable ver of wii u software it would take multiple trys to boot up correctly so i updated to 5.3.2 U but idk if smash stack is...
Quality and Characteristic: White crystal with no clumps, be able to absorb moisture and be soluted in water - Manufacturer - Producer - PSL176311YH
.The power of the Shabad Guru works with the responsiveness and subtlety of the mind-body system. By working with the Shabad, you can create great changes in your self from top to bottom, from cells to brain, and from feelings to moods.The rhythmic Naad of the Shabad Guru engages and synchronizes the flow of thoughts. This produces an inner stillness called shuniya or the
Ninety licensed drivers who meet the eligibility criteria will be recruited to participate in the study, which involves four visits, two at UI Hospitals and Clinics and two at the National Advanced Driving Simulator in Coralville.. The four visits will require a total of approximately nine to 10 hours to complete. Participants will be paid for their time and effort. Drivers who currently wear soft spherical contact lenses and who would like to participate in this or future studies can learn more by visiting http://www.drivingstudies.com or by calling the NADS hotline at 319-335-4719.. STORY SOURCE: University of Iowa News Services, 300 Plaza Centre One, Suite 371, Iowa City, Iowa 52242-2500. MEDIA CONTACTS: Timothy Brown, NADS, 319-335-4785, [email protected]; Mark Wilkinson, NADS, 319-356-8301, [email protected]; Gary Galluzzo, University News Services, 319-384-0009, [email protected] ...
Nov. 4, 2010. At A Glance. College of Engineering participates in USA Science and Engineering Festival. Representatives of the University of Iowa College of Engineering recently participated in the first USA Science and Engineering Festival Oct. 23-24 in Washington, D.C.. Participants included Omar Ahmad, assistant director, and Andrew Veit, MiniSim manager, both from the National Advanced Driving Simulator (NADS). Also attending were David Rethwisch, professor of chemical and biochemical engineering and director of Project Lead the Way for Iowa; Becca Whitaker, Engineering K-12 coordinator; and Kandace Munson, Project Lead the Way coordinator.. NADS staff were invited by the Transportation Research Board of the National Research Council to discuss and demonstrate the dangers of distracted driving at the festival on the National Mall. The MiniSim was driven by over 300 attendees, and nearly 10,000 people passed through the National Research Councils tent during the weekend. On Sunday, Oct. 24, ...
No Compromises sez: America is dead! Our Constitutional rights are gone and no body cares! What will it take before TRUE Americans stand up and fight back against this tyranny? AMERICAN JOURNALIST COMMITS FLAGRANT HATE CRIME By Paul L. Williams (author of The Day of Islam) Gerardo Sandoval, I hate you! I hate you and…
Esalah, J. O., Weber, M. E. and Vera, J. H. (2000), Removal of lead, cadmium and zinc from aqueous solutions by precipitation with sodium Di-(n-octyl) phosphinate. Can. J. Chem. Eng., 78: 948-954. doi: 10.1002/cjce.5450780512 ...
The transcription factor CtBP associates with transcriptional repressors for the regulation of genes involved in development, cell cycle regulation, and transformation. Zhang et al. show that the corepressor activity of CtBP can be regulated by the redox state of the cell. Mammalian CtBP is regulated by physiological concentrations of nuclear nicotinamide adenine dinucleotides (NADs). When the nuclear concentration of free NAD and NADH increases, CtBP increases its affinity for viral and cellular repressors and represses transcription. This regulatory mechanism suggests how protein interactions might respond to metabolic balances for the regulation of transcription.. Q. Zhang, D. W. Piston, R. H. Goodman, Regulation of corepressor function by nuclear NADH. Science 295, 1895-1897 (2002). [Abstract] [Full Text]. ...
A 6-year-old student at a Queens public school has been hospitalized with symptoms of meningitis, health officials said yesterday. The boy was hospitalized...
Margot....oh my little caboose. The girl has more energy than my other children combined. She NEVER stops. She is constantly in motion and when she is strapped into her car seat she is a jibber jabbering away. She cannot stop moving. Its different. Her favorite things to do these days are to climb...anything. To shove stuff in places they dont belong. I found a sippy cup with week old milk in the crystal cabinet. A cabinet that I dont frequent often and therefore I was lucky to find this sippy full of yucky goodness. She is talking alot. Her favorite thing to say is Noah....O-wah! She repeats everything her big sister says and trys to say Meredith, but Ta-ta works for now. She went to the pediatrician for her 15 month check up. Besides her constant runny nose and changing her thyroid dosage again, she is the picture of health. I just love this one to pieces. Melts her Mommas heart. She loves to cuddle and give kisses. She loves to wave and say bye-bye. She claps for herself when she is proud ...
Solvay is an advanced materials and specialty chemicals company offering a portfolio of more than 2000 products across various key markets worldwide. ...
1HOS: Inhibition of human immunodeficiency virus-1 protease by a C2-symmetric phosphinate. Synthesis and crystallographic analysis.
Sorry again for the millions of qns but Im doing quite badly in this subject so I need loads of help! Could you just check my answer for this qn? A lacI mutatio
Weather History for Oakey (Military), QNS - Get Weather History data by the day, week, month, or year with details like temperature, humidity, wind, etc. on Weather Underground.
Weather History for Hamilton Island, QNS - Get Weather History data by the day, week, month, or year with details like temperature, humidity, wind, etc. on Weather Underground.
Z-AKR-AMC | Z-Ala-Lys-Arg-AMC 877-03 5 mg | 215.00 EURBenzyloxycarbonyl-L-alanyl-L-lysyl-L-arginine-4-methyl-coumaryl-7-amide (M.W.
Vitamin D is a steroid hormone precursor that is associated with a range of human traits and diseases. Previous GWAS of serum 25-hydroxyvitamin D concentrations have identified four genome-wide significant loci (GC, NADSYN1/DHCR7, CYP2R1, CYP24A1). In this study, we expand the previous SUNLIGHT Consortium GWAS discovery sample size from 16,125 to 79,366 (all European descent). This larger GWAS yields two additional loci harboring genome-wide significant variants (P = 4.7x10(-9) at rs8018720 in SEC23A, and P = 1.9x10(-14) at rs10745742 in AMDHD1). The overall estimate of heritability of 25-hydroxyvitamin D serum concentrations attributable to GWAS common SNPs is 7.5%, with statistically significant loci explaining 38% of this total. Further investigation identifies signal enrichment in immune and hematopoietic tissues, and clustering with autoimmune diseases in cell-type-specific analysis. Larger studies are required to identify additional common SNPs, and to explore the role of rare or ...
EC 6. Ligases. Ligases are enzymes that catalyse the joining of two molecules with concomitant hydrolysis of the diphosphate bond in ATP or a similar triphosphate. Ligase is commonly used for the common name, but, in a few cases, synthase or carboxylase is used. Synthetase may be used in place of synthase for enzymes in this class.. EC 6.1 Forming Carbon-Oxygen Bonds. To this subclass belong the enzymes acylating a transfer RNA with the corresponding amino acid (amino-acid-tRNA ligases) (EC 6.1.1).. EC 6.2 Forming Carbon-Sulfur Bonds. To this subclass belong the enzymes synthesizing acyl-CoA derivatives.. EC 6.3 Forming Carbon-Nitrogen Bonds. To this subclass belong the amide synthases (EC 6.3.1), the peptide synthases (EC 6.3.2), enzymes forming heterocyclic rings (EC 6.3.3), enzymes using glutamine as amido-N-donor (EC 6.3.5) and a few others (EC 6.3.4).. EC 6.4 Forming Carbon-Carbon Bonds. These are the carboxylating enzymes, mostly biotinyl-proteins.. EC 6.5 Forming Phosphoric ...
Shanehttp://picasaweb.google.com/LandDCsurvey/DropBox?authkey=Gv1sRgCLL1npCy9uCKZQ#5459284767935499922Yes…the portrayal as nasty racist lunatics is completely uncalled for! Im fairly certain Elie Wiesel would be ecstatic beyond words if the Teabaggers description of the holocaust was accurate. Frankly as far as Im concerned goat fucking would be a step up for them. At least they wouldnt be raping corpses anymore. Fucking vulturous pricks. I cant stand watching these bastards claw through the ashes to drag up victims of actual true blue fascism and popularist mania to protest FUCKING HEALTH CARE REFORM. This shit overblows HCR while belittling the actual holocaust. They might as well just go around and kick every Jew and Poll right in the nads. My grandfather and great uncle did NOT join the military and go back to Europe to fight HEALTH CARE REFORM. My relatives in Poland were not killed by HEALTH CARE REFORM. Sorry for flying off the handle but I feel strongly about this. These people ...
Since heterotrophic prokaryotes play an important biogeochemical role in aquatic ecosystems and have a high capacity to survive in extreme environments, easy-to-perform protocols that probe their physiological states and the effects of environmental variables on those states are highly desired. Some methodologies combine a general nucleic acid stain with a membrane integrity probe. We calibrated one of these, the nucleic acid double-staining (NADS) protocol (G. Gre ́gori, S. Citterio, A. Ghiani, M. Labra, S. Sgorbati, S. Brown, and M. Denis, Appl. Environ. Microbiol. 67:4662-4670, 2001), determining the optimal stain concentrations in seawater and the response to conditions that generate prokaryote death (such as heat) and to conditions that are known to produce death in plankton, such as nutrient limitation or flagellate grazing. The protocol was validated by comparison to two methods used to detect viability: active respiration by 5-cyano 2,3-ditolyl tetrazolium chloride (CTC) and ...
... The removal of Ni(II) by foam fractionation is brought about by the electrostatic mechanism. From removal and zeta potential measurements, it clearly demonstrates that Ni**2** plus and NiOH** plus ions become associated with the dodecylsulfonate coated (negative charge) N//2 gas bubbles. The optimal Ni(II) to NaDS (molar ratio) is 0. 2. The Ni**2** plus species forms moderately strong complexes with dodecylsulfonate ions.
Based in Brussels, Belgium, EUD is a not-for-profit European non-Governmental organisation (ENGO) whose members comprise of National Associations of the Deaf (NADs).
Behind schedule and costing about $60 million so far, the government-owned National Advanced Driving Simulator (NADS) operated by the University of Iowa is about to come online. Its sponsors may be gearing up to celebrate, but others still are asking the question that has plagued the project from the start: Why is so much money being spent on something with no proven value for highway safety research?. A longtime skeptic is Institute president Brian ONeill (see "NHTSA moves ahead with $49 million driving simulator," April 20, 1996). "Not only was this simulator expensive to build, it will cost a lot to operate," he points out. "No matter how sophisticated it is, the subjects in it will know theyre being studied, so researchers will never know if theyre observing normal behavior or, whats more likely, special behavior. Simulators violate a basic principle of behavioral research - people often behave differently when they know theyre being observed.". Leonard Evans, recently retired senior ...
Wisconsin German Shorthaired Pointer Rescue WGSPR Inc. - GSPs & Addisons Disease - The mission of WGSPR is: To provide the general public with knowledge of our breed, offer fully vetted, rescued, purebred GSPs for adoption, with a life-time follow-up and return policy for all of our adopted GSPs.
In Silico Analyses of Epicoccamides on Selected Leishmania Trypanothione Reductase Enzyme-based Target, Nighat Fatima, Amara Mumtaz1, Rahat Shamim2, M. I. Qadir3 and S. A. Muhammad
Resolving the important cell biology question of which agonist directly activates TRPM2 is the goal of an elegant new report in the May 2015 issue of The Journal of General Physiology by Tóth, Iordanov, and Csanády (Tóth et al., 2015). Previous work by this group determined that enzymatically purified AMP and cADPR added to inside-out excised membrane patches did not, in fact, activate TRPM2 (Tóth et al., 2014). Using available commercial tools, such as a linear nucleotide-specific pyrophosphatase, the authors showed that apparent TRPM2 activation by cADPR was actually caused by the presence of contaminant ADPR (Tóth and Csanády, 2010).. To resolve the question of whether pyridine dinucleotides directly activate TRPM2, Tóth, Iordanov, and Csanády tested the effects of pyridine dinucleotides to inside-out membrane patches expressing TRMP2 channels. Their experiments showed that increasing concentrations of NAD, NAAD, NAADP, and ADPR all activated TRPM2 channels, albeit with different ...
I came across your blog when I was searching for maternity patterns and clothes to make and I have been looking in ever since (I am 33 weeks pregnant with baby girl #2). Anyway I have a question for you and I hope you can answer me as I have asked qns on other peoples blogs and recieved no answer. I was wondering how you transfer you patterns, Im guessing you dont cut your original pattern pieces and Im not sure how to transfer the pattern onto another source (paper?calico?tracing paper?) Well I hope you can help me as you seem like such a pro ...
It all started in the 1950s when the basketball team called itself the Nads. The hockey team also adopted the same name in the 70s. In 2000 the basketball named changed to the Balls. Still going with the same obvious theme. We figured Scrotie was a natural compliment that worked for both teams.. When was Scrotie introduced? ...
Background: Alteration of cellular metabolism is a hallmark of cancer, which underlies exciting opportunities to develop effective, anti-cancer therapeutics thr
Young Barre Phillips plays lika "a pioneer of free improvisation by wood-base" here. He trys various aspects of wood-base as "sound maker", as well as musical imagination ...
Much of Harpers obsession with pleasing Israel and punishing anyone who criticizes it as anti-semetic is rooted in his Evangelical christian beliefs. He trys keeping his religion under wraps, but he in fact is a strong believer. This is not just about getting the jewish vote. The extent to which hes supporting Israel is way beyond necessity and it feels more like a crusade then a political decision. Get this guy out before he turns us into a theocracy. He\s also a complete buffoon. Internationally they think him a hick. Now he is trying through alternate roots to shove his religion down our collective throats. This inept bozo has no respect for Canadians. I for one and getting really weary of him!. ReplyDelete ...
If you have used this database, please ensure that you acknowledge this most recent Pseudomonas Genome Database publication rather than just the website URL. Thank you!. Winsor GL, Griffiths EJ, Lo R, Dhillon BK, Shay JA, Brinkman FS (2016 ...
NMNAT2小鼠单克隆抗体(ab56980)可与小鼠, 人样本反应并经WB, IHC, Flow Cyt, ICC/IF实验严格验证,被2篇文献引用并得到1个独立的用户反馈。
Dr. Jan Ole Kriegs (left) guided us through the exhibition, the entrance was made up in a really interesting way, starting to explain what bionic is in a way that attract children as well as adults. I am so glad they didnt build such an awful oldschool exhibition. ^^ The bear trys to sell a rake as his invention by sticking some claws at it, but the secretary (bird) doesnt buy it ...
I am not sure why everyone is trying to take this as a call to rush into the streets blasting. I just wonder when everone is going to realize that they simply dont care what we have to say. That our demands may be heard, but will never be met unless they are forced. Everyone trys to crypticly talk about "they are ready" "were all just hunkered down waiting" "our numbers are growing" "more are waking up everyday" blah blah blah. Look around, what are you people waiting for? What is breaking point? When the 20,000 troops deploy here? Martial law? What? Not one gunfight broke out over weapons confinscation in New Orleans. Sure there may have been gunfire and shootings, but who resisted the gun round up.....? I predict the same situations should it happen nation/state wide. No uprising, no resistance, just ok here is my weapon...take me to shelter. Most of you cant even maintain eye contact with a cop for more than five seconds, let alone tell a uniformed/armed soilder that is at your door you ...
Tubulin heterogeneity in the trypanosome Crithidia fasciculata.: The interphase cell of Crithidia fasciculata has three discrete tubulin populations: the subpel
The Angry Gorilla is when a man shaves his pubic hairs and saves them in a bag next to his bed. the next time he has a woman over, after sex when he ejaculates on her chest, he then proceeds to take the saved pubic hairs and throw them at her chest. She then trys to pull them off looking similar to a gorilla pounding on his chest.
Specific specimen requirements for each test are listed in the Test Center. Specimen requirements include information such as specimen volume, collection and transport containers as well as transport temperature. If additional information is needed for the interpretation of the test results or there are specific instructions for patient preparation, they are listed along with specimen requirements. It is critical that an adequate specimen volume is submitted for analysis. The volume requested in this directory is enough for initial analysis as well as for any confirmatory tests that must be performed. If an inadequate specimen is submitted, we may not be able to perform the initial test or required confirmatory procedures. If repeat or confirmatory tests cannot be performed, the report will indicate that the specimen quantity submitted was "QNS" (Quantity Not Sufficient) for additional testing. When serum or plasma is to be submitted for analysis, it is good practice to collect a volume of blood ...
Wiese, J., Schulz, D., Stöhr, R., Nagel, K., Labes, A., Schneemann, I., Heindl, H., Jansen, N., Silber, J., Kramer, A., Goecke, F. R., Schmaljohann, R., Staufenberger, T., Gärtner, A., Baumann, H. I., Kleinschmidt, K. and Imhoff, J. F. (2011) Biologically active compounds from microorganisms derived from marine macroorganisms and marine sediments. [Poster] In: NatPharma: Nature Aided Drug Discovery, NADD. , 05.-09.06.2011, Napoli, Italy ...
Modr k >> Asi nejsme v rozporu. V , v literatu e sice nade i grafy, kter maj demonstrovat spektr ln citlivost ryb ho oka a zn m je. Ty grafy vych zej ze slo en sv tlocivn ch bun k ryb s tnice. Ale najde i n zor, e ryba je prakticky barvoslep , e v praxi rozli uje jen odst ny ed . I o b c ch se tvrd , e je p iv d v amok erven fangle, ale i e jsou barvoslep . Psi barevn m vid n m dajn tak nedispoznuj (m m 3 a zept m se jich na to).U nap klad generalizovat o vid n ryb je asi absurdn . Zcela jist se to u r zn ch druh li . raloci jsou sice paryby a v vojov velmi sta a je to o n em jin m, ale ned vno jsem vid l film o kladivounech, kte vid o ima houbeles a sv t "vid " sn m n m elektrick ho pole (senzory jsou pr bv po stran ch kladivov hlavy) a orientuj se i v oce nu podle miziv ch rozd lu elektrick ho pole (vliv na to m slo en zemsk k ry, tektonick poruchy atd.). A jest to pou vaj p i migraci v oce nu jako my GPS. ...
Phosphinates or hypophosphites are a class of phosphorus compounds conceptually based on the structure of hypophosphorous acid. IUPAC prefers the term phosphinate in all cases, however in practice hypophosphite is usually used to describe inorganic species (e.g. sodium hypophosphite), while phosphinate typically refers to organophosphorus species. The hypophosphite ion is H 2PO− 2. The salts are prepared by heating white phosphorus in warm aqueous alkali e.g. Ca(OH)2: P4 + 2 Ca(OH)2 + 4 H2O → 2 Ca(H2PO2)2 + 2 H2 Hypophosphites are reducing agents: H 2PO− 2 + 3OH− → HPO2− 3 + 2H2O + 2e− Hypophosphites are used in electroless nickel plating as the reducing agent to deposit for example Ni metal from Ni salts. The hypophosphite ion is thermodynamically unstable, and disproportionates on heating to phosphine gas and phosphate salts: 2 H 2PO− 2 → PH3 + HPO2− 4 Organophosphinates are organophosphorus compounds with the formula OP(OR)R2. They can be synthetized by the ...
In this study, the association of GC, CYP2R1, and DHCR7/NADSYN1 polymorphisms with vitamin D deficient rickets in Chinese subjects was reported for the first time. In this population-based sample, we analyzed the association of 12 loci of three vitamin D-related genes with rickets in Han children from Northeast China. These results suggest that GC and CYP2R1 variants are important contributors to susceptibility to rickets in Chinese children.. GC, also known as vitamin D-binding protein (DBP), and it is a member of the albumin family [23], encodes DBP synthesized in the liver and transports vitamin D and its metabolites. Recently, studies investigated the association between GC polymorphisms and 25(OH)D status [24-28]. Because certain mutations in the GC gene are known to cause defects in GC function, we hypothesized that GC polymorphisms can predict susceptibility to developing rickets. In the present study, the strongest association for developing rickets was found to be with a variant at ...
Axonal degeneration is an active program of self-destruction that is observed in many physiological and pathological settings. In Wallerian degeneration slow (wlds) mice, Wallerian degeneration in response to axonal injury is delayed because of a mutation that results in overexpression of a chimeric protein (Wlds) composed of the ubiquitin assembly protein Ufd2a and the nicotinamide adenine dinucleotide (NAD) biosynthetic enzyme Nmnat1. We demonstrate that increased Nmnat activity is responsible for the axon-sparing activity of the Wlds protein. Furthermore, we demonstrate that SIRT1, a mammalian ortholog of Sir2, is the downstream effector of increased Nmnat activity that leads to axonal protection. These findings suggest that novel therapeutic strategies directed at increasing the supply of NAD and/or Sir2 activation may be effective for treatment of diseases characterized by axonopathy and neurodegeneration. ...
In the present study we established that NAMPT is the molecular target of the GMX1778 small-molecule cancer therapeutic candidate. A global metabolic profiling study was instrumental in the discovery that NAD+ levels were rapidly depleted in GMX1778-treated cells. NAD+ depletion was followed by ATP depletion and ultimately resulted in cell death. Previous results suggested that the mechanism of action of GMX1778 involved the inhibition of IKK leading to decreased NF-κB activity (24). However, our results show that the inhibition of IKK and the subsequent inhibition of NF-κB activity in cells following GMX1778 treatment are secondary to the initial NAD+ depletion, since it can be recovered by restoration of NAD+ levels. Furthermore, NAD+ repletion completely rescues cells from the cytotoxicity of GMX1778. There appears to be a threshold level of NAD+ required to protect cells from GMX1778 cytotoxicity, given that NMN supplementation only partially restores the cellular NAD+ levels while ...
Exolit OP 1240 is a flame retardant based on an organic aluminium phosphinate which is a thermally stable white solid without any hazard classification or labeling. It can be easily compounded into polyesters and subsequently processed again to manufacture flame retarded parts used in consumer or industrial products. For more details see our Innovation Spotlight video.
Wiese, J., Schulz, D., Stöhr, R., Nagel, K., Labes, A., Schneemann, I., Heindl, H., Jansen, N., Silber, J., Kramer, A., Goecke, F. R., Schmaljohann, R., Staufenberger, T., Gärtner, A., Baumann, H. I., Kleinschmidt, K. and Imhoff, J. F. (2011) Biologically active compounds from microorganisms derived from marine macroorganisms and marine sediments. [Poster] In: NatPharma: Nature Aided Drug Discovery, NADD. , 05.-09.06.2011, Napoli, Italy ...
Looking for amidase? Find out information about amidase. Any enzyme that catalyzes the hydrolysis of nonpeptide C=N linkages Explanation of amidase
Natural products containing carbon-phosphorous bonds, so-called C-P compounds, are derivatives of phosphonate and phosphinate with substitution of alkyl group for hydrogen of phosphorus-hydrogen bonds. C-P compounds have been found in many organisms, but only protists and bacteria, mostly Actinobacteria, have biosynthetic capacity. A common reaction in the biosynthetic pathway is C-P bond forming reaction from phosphoenolpyruvate (PEP) to phosphonopyruvate (PnPy) catalyzed by PEP phosphomutase. 2-Aminoethylphosphonate (AEP) is the most abundant C-P compound in the natural world. AEP derivatives include phosphonoprotein, phosphonoglycan, and phosphonolipid. Other known C-P compounds are bioactive substances used in medicine (antibiotics) and agriculture (herbicide) such as fosfomycin, FR-33289, rhizocticin, and bialaphos ...
I just found this while looking for others going through similar situations and my heart is breaking for each of you- for ALL of us. I too have a daughter who recently turned 18. She has become obsessed with the celebrity Eugenia Cooney who I believe is a fashion model and trys to emulate her in every way, from her pattern of speech, clothing, and appearance in general. I noticed my daughters weight fluctuating dramatically during her sophomore and junior year of high school, and she was spending a tremendous amount of time on her phone. To make a long story short, I was able to have a heart to heart with her after years of denial where she thank god finally told me the truth. She began following this Eugenia models career and spent her days watching videos of her, and said she became transfixed with her. When me and her father were not home she would obsessively print out photographs of her, and we discovered a binder just filled to the brim with photos which she kept in hidden in her school ...
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
Pearson, Spearman, and Kendall partial correlation.. PROC CORR also computes Cronbachs coefficient alpha for estimating reliability. The default correlation analysis includes descriptive statistics, Pearson correlation statistics, and probabilities for each analysis variable. You can save the correlation statistics in a SAS data set for use with other statistical and reporting procedures. Simple Correlation Analysis for a Fitness Study Using PROC CORR is the simplest form of PROC CORR output. Pearson correlation statistics are computed for all numeric variables from a study investigating the effect of exercise on physical fitness. The statements that produce the output follow ...
function nag_correg_robustm_corr_user_example indm = int64(1); x = [3.4, 6.9, 12.2; 6.4, 2.5, 15.1; 4.9, 5.5, 14.2; 7.3, 1.9, 18.2; 8.8, 3.6, 11.7; 8.4, 1.3, 17.9; 5.3, 3.1, 15; 2.7, 8.1, 7.7; 6.1, 3, 21.9; 5.3, 2.2, 13.9]; a = [1; 0; 1; 0; 0; 1]; theta = [0; 0; 0]; user = [4, 2]; [user, covar, aOut, wt, thetaOut, nit, ifail] = ... nag_correg_robustm_corr_user(@ucv, indm, x, a, theta, user, user) function [userp, u, w] = ucv(t, userp) cu = userp(1); u = 1.0; if (t ~= 0) t2 = t*t; if (t2 , cu) u = cu/t2; end end % w function and derivative cw = userp(2); if (t , cw) w = cw/t; else w = 1.0; end ...
MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersThiaminethiazole biosynthesis adenylyltransferase ThiF (TIGR02356; EC 2.7.7.73; HMM-score: 12.4) ...
MetabolismCentral intermediary metabolismSulfur metabolismsulfate adenylyltransferase, large subunit (TIGR02034; EC 2.7.7.4; HMM-score: 17) ...
Unprecedented Result from NAFEMS World Congress 2015 Call for Papers Over 400 Abstracts Received for the International Congress on Simulation Technology, Incorporating the 2nd International Conference on Simulation Process & Data Management. GLASGOW, UK, 18th December 2014 - NAFEMS, the International Association for the Engineering Analysis Community, has announced that over 400 abstracts have been submitted for its 2015 World Congress in San Diego, USA - the highest response level y
Origin of bacteria in the clean room and their growth requirements. Trypanothione (TSH) is over- produced in Leishmania by the higher activity of the rate-limiting enzymes О-glutamyl cysteine synthetase (ОGCS) and ornithine decar- boxylase (ODC).
Evaluation of selected antitumor agents as subversive substrate and potential inhibitor of trypanothione reductase: an alternative approach for chemotherapy of ...
Fuck you for making me do that. As for GSP ducking Diaz, lets talk to Kos eye socket about that. Or, lets talk to Fitch or BJ Penn about the way GSP beat them to within an inch of their lives. These guys even got rematches, and nobody has ever beaten GSP in a rematch. Quite the opposite: a rematch with GSP has equalled certain failure for every opponent who ever got one ...
Carol Binnion examines the Family Courts disposal of care proceedings in what was originally thought to be a case of non-accidental injury.. The case of Re Effie Stillwell (A Child) (sub nom. Re F (A Child); F v Buckinghamshire CC) (Family Court) Her Honour Judge Venables 26.4.2017 highlights how parents can be believed to have inflicted serious physical injuries on their child when, in fact, there is an underlying rare genetic condition causative of the injuries in the course of normal handling.. E was presented at hospital in August 2016 aged approximately six months by parents who reported a collapse. E was unresponsive and had difficulty breathing. On examination she was found to have subdural and retinal haemorrhages and encephalopathy-the well-known triad of injuries associated with shaken baby syndrome.. However, there were no external injuries of note and none of the injuries frequently found in children who have been shaken or suffered abusive head trauma, such as:. a. Bruising to the ...
Learn about stem cell transplant for acute lymphocytic leukemia (ALL), which replaces blood-forming stem cells when they or the bone marrow have been damaged.

trypanothione synthetase
     Summary Report | CureHuntertrypanothione synthetase Summary Report | CureHunter

... member of ATP-dependent class of ligases forming an amide linkage ... spermidine synthase and trypanothione synthetase, f) some of ... member of ATP-dependent class of ligases forming an amide linkage ... spermidine synthase (SpdS), trypanothione synthetase (TryS or ... spermidine synthase and trypanothione synthetase; D) Inhibition of the glycolytic enzyme PPi-dependent phosphofructokinase (PFK ... TSA), trypanothione reductase (TryR or TR), tryparedoxin peroxidase (TXNPx), deoxyhypusine synthase (DHS) and deoxyhypusine ...
more infohttp://www.curehunter.com/public/keywordSummaryC064407-trypanothione-synthetase.do

NAD Synthetase from B. subtilis, Recombinant - Creative EnzymesNAD Synthetase from B. subtilis, Recombinant - Creative Enzymes

... a NAD+ synthase (EC 6.3.1.5) is an enzyme that catalyzes the chemical reaction:ATP + deamido-NAD+ + NH3↔ AMP + diphosphate + ... amide synthases). This enzyme participates in nicotinate and nicotinamide metabolism and nitrogen metabolism. ... In enzymology, a NAD+ synthase (EC 6.3.1.5) is an enzyme that catalyzes the chemical reaction:ATP + deamido-NAD+ + NH3↔ AMP + ... EC 6.3.1.5; 9032-69-3; NAD+ synthetase; NAD+ synthase; nicotinamide adenine dinucleotide synthetase; diphosphopyridine ...
more infohttps://www.creative-enzymes.com/product/NAD-Synthetase-From-B-Subtilis-Recombinant_3437.html

پروتئین - ویکی‌پدیا، دانشنامهٔ آزادپروتئین - ویکی‌پدیا، دانشنامهٔ آزاد

Glycolysis → Pyruvate decarboxylation → Citric acid cycle → Oxidative phosphorylation (electron transport chain + ATP synthase) ... which forces the CO-NH amide moiety into a fixed conformation.[1] The side chains of the standard amino acids, detailed in the ...
more infohttps://fa.wikipedia.org/wiki/%D9%BE%D8%B1%D9%88%D8%AA%D8%A6%DB%8C%D9%86?match=en

Chemical validation of trypanothione synthetase: a potential drug target for human trypanosomiasis. | CureHunterChemical validation of trypanothione synthetase: a potential drug target for human trypanosomiasis. | CureHunter

Amide Synthases (antagonists & inhibitors, genetics, metabolism) *Animals. *Antiprotozoal Agents (chemistry, pharmacokinetics, ...
more infohttp://www.curehunter.com/public/pubmed19828449.do

KEGG BRITE: Enzymes - Bacillus anthracis CDC 684KEGG BRITE: Enzymes - Bacillus anthracis CDC 684

6.3.1 Acid-D-ammonia (or amine) ligases (amide synthases) 6.3.2 Acid-D-amino-acid ligases (peptide synthases) ... 6.3.5.9 hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing) 6.3.5.10 adenosylcobyric acid synthase (glutamine- ... 6.3.5.7 glutaminyl-tRNA synthase (glutamine-hydrolysing) BAMEG_0380 gatA; glutamyl-tRNA(Gln) amidotransferase, A subunit BAMEG_ ... 6.3.5.6 asparaginyl-tRNA synthase (glutamine-hydrolysing) BAMEG_0380 gatA; glutamyl-tRNA(Gln) amidotransferase, A subunit BAMEG ...
more infohttps://www.genome.jp/kegg-bin/get_htext?bah01000+BAMEG_0379

Lies, Damned Lies, Statistics, and Probability of
Abiogenesis CalculationsLies, Damned Lies, Statistics, and Probability of Abiogenesis Calculations

6] Wiegand TW, Janssen RC, and Eaton BE, Selection of RNA amide synthases. Chem Biol, 4: 675-83, 1997 ...
more infohttp://www.talkorigins.org/faqs/abioprob/abioprob.html

Keating TA[au] - PubMed - NCBIKeating TA[au] - PubMed - NCBI

Vibriobactin biosynthesis in Vibrio cholerae: VibH is an amide synthase homologous to nonribosomal peptide synthetase ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed?cmd=search&term=Keating+TA%5Bau%5D&dispmax=50

ENZYME search by enzyme classENZYME search by enzyme class

6. 3. 1.- Acid--ammonia (or amine) ligases (amide synthases). 6. 3. 2.- Acid--amino-acid ligases (peptide synthases). 6. 3. 3.- ... 3. 5. 1.- In linear amides. 3. 5. 2.- In cyclic amides. 3. 5. 3.- In linear amidines. 3. 5. 4.- In cyclic amidines. 3. 5. 5.- ... 4. 3. 2.- Lyases acting on amides, amidines, etc. 4. 3. 3.- Amine-lyases. 4. 3.99.- Other carbon-nitrogen lyases. 4. 4. -.- ... 6. 1. 2.- Acid--alcohol ligases (ester synthases). 6. 1. 3.- Cyclo-ligases. 6. 2. -.- Forming carbon-sulfur bonds. 6. 2. 1.- ...
more infohttps://enzyme.expasy.org/enzyme-byclass.html

Fabclavine biosynthesis in X. szentirmaii: shortened derivatives and characterization of the thioester reductase FclG and the...Fabclavine biosynthesis in X. szentirmaii: shortened derivatives and characterization of the thioester reductase FclG and the...

Keating TA, Marshall CG, Walsh CT (2000) Vibriobactin biosynthesis in Vibrio cholerae VibH is an amide synthase homologous to ... Brachmann AO, Joyce SA, Jenke-Kodama H, Schwär G, Clarke DJ, Bode HB (2007) A type II polyketide synthase is responsible for ...
more infohttps://rd.springer.com/article/10.1007%2Fs10295-018-02124-8

The Kirschning Group - PublicationsThe Kirschning Group - Publications

Broad substrate specificity of the amide synthase in S. hygroscopicus- new 20-membered macrolactones derived from geldanamycin ...
more infohttp://kirschning-group.com/publications.html

The Universal Plausibility Metric (UPM) & Principle (UPP) | Theoretical Biology and Medical Modelling | Full TextThe Universal Plausibility Metric (UPM) & Principle (UPP) | Theoretical Biology and Medical Modelling | Full Text

Wiegand TW, Janssen RC, Eaton BE: Selection of RNA amide synthases. Chem Biol. 1997, 4: 675-683. 10.1016/S1074-5521(97)90223-4. ...
more infohttps://tbiomed.biomedcentral.com/articles/10.1186/1742-4682-6-27

Vibriobactin - WikipediaVibriobactin - Wikipedia

VibH Is an Amide Synthase Homologous to Nonribosomal Peptide Synthetase Condensation Domains". Biochemistry. 39: 15514. ...
more infohttps://en.wikipedia.org/wiki/Vibriobactin

Frontiers | PR Toxin - Biosynthesis, Genetic Regulation, Toxicological Potential, Prevention and Control Measures: Overview and...Frontiers | PR Toxin - Biosynthesis, Genetic Regulation, Toxicological Potential, Prevention and Control Measures: Overview and...

PR amide, and PR acid, based on conditions available reflects unstability and degradative aspects. Since the PR toxin-induced ... PR amide (EreE), through reactions with amino group of the amino acids and catalyzed by PR amide synthase (Figure 1; Chang et ... Chang, S. C., Cheng, M. K., and Wei, Y. H. (2004a). Production of PR-imine, PR acid, and PR-amide relative to the metabolism of ... Indeed, in these cases, PR oxidase, PR-amide synthetase and eremofortin C oxidase from P. roqueforti catalyze the conversion of ...
more infohttps://www.frontiersin.org/articles/10.3389/fphar.2018.00288/full

Unique core genomes of the bacterial family vibrionaceae: Insights into niche adaptation and speciationUnique core genomes of the bacterial family vibrionaceae: Insights into niche adaptation and speciation

Vibriobactin biosynthesis in Vibrio cholerae: VibH is an amide synthase homologous to nonribosomal peptide synthetase ...
more infohttps://pub.uni-bielefeld.de/publication/2500959

4-methyleneglutamate-ammonia ligase - Wikipedia4-methyleneglutamate-ammonia ligase - Wikipedia

... amide synthases). The systematic name of this enzyme class is 4-methylene-L-glutamate:ammonia ligase (AMP-forming). This enzyme ... Winter HC, Su TZ, Dekker EE (1983). "4-methyleneglutamine synthetase: a new amide synthetase present in germinating peanuts". ...
more infohttps://en.wikipedia.org/wiki/4-methyleneglutamate%E2%80%94ammonia_ligase

Aspartate-ammonia ligase - WikipediaAspartate-ammonia ligase - Wikipedia

... amide synthases). The systematic name of this enzyme class is L-aspartate:ammonia ligase (AMP-forming). Other names in common ...
more infohttps://en.wikipedia.org/wiki/Aspartate%E2%80%94ammonia_ligase

US5705502A - Pharmacologically active pyrimidineamine derivatives and processes for the preparation thereof 
        - Google...US5705502A - Pharmacologically active pyrimidineamine derivatives and processes for the preparation thereof - Google...

Amide derivatives as glycogen synthase kinase 3-beta inhibitors WO2003047516A2 (en) 2003-06-12. Pyrimidine compounds ... Heteroaryl amines as glycogen synthase kinase 3β inhibitors (GSK3 inhibitors) US5656629A (en) 1997-08-12. 6-substituted ... Heteroaryl amines as glycogen synthase kinase 3beta inhibitors(GSK inhibitors) ES2312749T3 (en) 2002-02-01. 2009-03-01. ...
more infohttps://patents.google.com/patent/US5705502?oq=5%2C815%2C488

SEQUENCES FOR FK228 BIOSYNTHESIS AND METHODS OF SYNTHESIZING FK228 AND FK228 ANALOGS - Patent applicationSEQUENCES FOR FK228 BIOSYNTHESIS AND METHODS OF SYNTHESIZING FK228 AND FK228 ANALOGS - Patent application

... synthase rather than an amide synthase) in cereulide and valinomycin biosynthesis in actinomycetes (Magarvey, Ehling-Schulz et ... KS, β-ketoacyl synthase; E, epimerase. cNN, not named. dNA, not available. TABLE-US-00003 TABLE 3 Deduced functions of open ... Abbreviations are as follows: AL, acyl coenzyme A ligase; KS, β-ketoacyl synthase; E, epimerase. [0025]FIG. 3 depicts the ... 150c CV_3386 AAQ61050 87/93 C. violaceum ATCC 16S rRNA pseudouridine synthase 12472 orf2 163 CV_3385 AAQ61049 66/76 C. ...
more infohttp://www.patentsencyclopedia.com/app/20100261878

Draft genome sequence of Streptomyces hyaluromycini MB-PO13T, a hyaluromycin producer | Standards in Genomic Sciences | Full...Draft genome sequence of Streptomyces hyaluromycini MB-PO13T, a hyaluromycin producer | Standards in Genomic Sciences | Full...

... the cluster also encodes amide synthase (Orf1-763), 5-aminolevulinate synthase (Orf1-762) and AMP-dependent synthase (Orf1-761 ... 5-Aminolevulinate synthase (Orf1-762), 5-aminolevulinate CoA ligase (Orf1-761) and amide synthase (Orf1-763) are involved in ... BiosynthesisC5NPolyketide synthaseRubromycin Streptomyces Introduction. Hyaluromycin is a hyaluronidase inhibitor isolated from ... Evolution of cyclizing 5-aminolevulinate synthases in the biosynthesis of actinomycete secondary metabolites: outcomes for ...
more infohttps://standardsingenomics.biomedcentral.com/articles/10.1186/s40793-017-0286-7

Similar articles for PubMed (Select 22318159) - PubMed - NCBISimilar articles for PubMed (Select 22318159) - PubMed - NCBI

Aromatic reduced amide bond peptidomimetics as selective inhibitors of neuronal nitric oxide synthase. ... Novel inhibitors of nitric oxide synthase with antioxidant properties.. Salerno L, Modica MN, Romeo G, Pittalà V, Siracusa MA, ... Reduced amide bond peptidomimetics. (4S)-N-(4-amino-5-[aminoakyl]aminopentyl)-N-nitroguanidines, potent and highly selective ... Nitric oxide synthase isoform inhibition before whole body ischemia reperfusion in pigs: vital or protective? ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed?LinkName=pubmed_pubmed&from_uid=22318159

Mechanism for nitrogen isotope fractionation during ammonium assimilation by Escherichia coli K12 | PNASMechanism for nitrogen isotope fractionation during ammonium assimilation by Escherichia coli K12 | PNAS

... glutamate synthase [glutamine(amide) 2-oxoglutarate amino transferase (GOGAT)], and (iv) glutamate dehydrogenase (GDH), the ... No isotopic fractionation is associated with GOGAT, because practically all of the amide N in gln is transferred to 2- ... 2000) The evolution of glutamate synthase. Mol Biol Rep 27(3):141-148. ... 2011) Expression and functional analysis of glutamate synthase small subunit-like proteins from archaeon Pyrococcus horikoshii ...
more infohttps://www.pnas.org/content/110/21/8696?ijkey=b3d7c48250d7ab8f96ed8118cdcbddda4c0da54e&keytype2=tf_ipsecsha

Aspartate-107 and leucine-109 facilitate efficient coupling of glutamine hydrolysis to CTP synthesis by Escherichia coli CTP...Aspartate-107 and leucine-109 facilitate efficient coupling of glutamine hydrolysis to CTP synthesis by Escherichia coli CTP...

Abbreviations used: CPS, carbamoyl phosphate synthase; CTPS, CTP synthase; GAT, glutamine amide transfer; GF-HPLC, gel- ... within the synthase domain of Escherichia coli CTP synthase. Mutant K102A CTP synthase exhibited wild-type activity with ... CTP synthase catalyses the ATP-dependent formation of CTP from UTP using either NH3 or l-glutamine as the nitrogen source. GTP ... L109F CTP synthase was prepared to block totally the putative NH3 tunnel; however, this enzymes rate of glutamine-dependent ...
more infohttp://www.biochemj.org/content/369/3/497

Organic Nitrates in Heart Failure Revisited | HypertensionOrganic Nitrates in Heart Failure Revisited | Hypertension

... or uncoupled NO synthases.1 Especially O2•− undergoes an avid reaction with NO• to yield peroxynitrite (ONOO−), one of the most ... originating from activated nicotine amide dinucleotide phosphate oxidases, a dysfunctional mitochondrial respiratory chain, ...
more infohttp://hyper.ahajournals.org/content/66/5/933

cysG - Siroheme synthase - Pseudomonas mendocina (strain ymp) - cysG gene & proteincysG - Siroheme synthase - Pseudomonas mendocina (strain ymp) - cysG gene & protein

S-adenosyl-L-methionine; via amide nitrogenUniRule annotation. ,p>Manual validated information which has been generated by the ... IPR028281. Sirohaem_synthase_central. IPR019478. Sirohaem_synthase_dimer_dom. IPR006367. Sirohaem_synthase_N. IPR003043. Uropor ... IPR028281. Sirohaem_synthase_central. IPR019478. Sirohaem_synthase_dimer_dom. IPR006367. Sirohaem_synthase_N. IPR003043. Uropor ... Siroheme synthaseAdd BLAST. 462. Amino acid modifications. Feature key. Position(s). DescriptionActions. Graphical view. Length ...
more infohttp://www.uniprot.org/uniprot/A4XUX3

DHS - Deoxyhypusine synthase - Brassica napus (Rape) - DHS gene & proteinDHS - Deoxyhypusine synthase - Brassica napus (Rape) - DHS gene & protein

NAD; via amide nitrogenBy similarity. 1. Binding sitei. 289. SpermidineBy similarity. 1. ... IPR002773 Deoxyhypusine_synthase. IPR036982 Deoxyhypusine_synthase_sf. IPR029035 DHS-like_NAD/FAD-binding_dom. ... IPR002773 Deoxyhypusine_synthase. IPR036982 Deoxyhypusine_synthase_sf. IPR029035 DHS-like_NAD/FAD-binding_dom. ... Belongs to the deoxyhypusine synthase family.Curated. Family and domain databases. Gene3D Structural and Functional Annotation ...
more infohttps://www.uniprot.org/uniprot/Q6RJS2
  • 3,5-Disubstituted indole derivatives as selective human neuronal nitric oxide synthase (nNOS) inhibitors. (nih.gov)
  • Discovery of N-(3-(1-methyl-1,2,3,6-tetrahydropyridin-4-yl)-1H-indol-6-yl) thiophene-2-carboximidamide as a selective inhibitor of human neuronal nitric oxide synthase (nNOS) for the treatment of pain. (nih.gov)
  • Novel, druglike 1,7-disubstituted 2,3,4,5-tetrahydro-1H-benzo[b]azepine-based selective inhibitors of human neuronal nitric oxide synthase (nNOS). (nih.gov)
  • Discovery of cis-N-(1-(4-(methylamino)cyclohexyl)indolin-6-yl)thiophene-2-carboximidamide: a 1,6-disubstituted indoline derivative as a highly selective inhibitor of human neuronal nitric oxide synthase (nNOS) without any cardiovascular liabilities. (nih.gov)
  • Design, synthesis, and biological evaluation of 3,4-dihydroquinolin-2(1H)-one and 1,2,3,4-tetrahydroquinoline-based selective human neuronal nitric oxide synthase (nNOS) inhibitors. (nih.gov)
  • The rapid conversion of PR toxin to the other derivatives such as PR imine, PR amide, and PR acid, based on conditions available reflects their unstability and degradative aspects. (frontiersin.org)
  • The present study showed that vitamin D levels were deficient in schizophrenics and had an inverse correlation between vitamin D levels, thyroid antibodies, inflammatory markers and homocysteine levels while positive correlation between nitric oxide, nitric oxide synthase and glutathione. (thefreedictionary.com)
  • The structure consists of a γ-rubromycin core possessing a C 5 N unit as an amide substituent of the carboxyl functionality. (biomedcentral.com)