A major dental enamel-forming protein found in mammals. In humans the protein is encoded by GENES found on both the X CHROMOSOME and the Y CHROMOSOME.
The proteins that are part of the dental enamel matrix.
A secreted matrix metalloproteinase that is the predominant proteolytic activity in the enamel matrix. The enzyme has a high specificity for dental enamel matrix protein AMELOGENIN.
The elaboration of dental enamel by ameloblasts, beginning with its participation in the formation of the dentino-enamel junction to the production of the matrix for the enamel prisms and interprismatic substance. (Jablonski, Dictionary of Dentistry, 1992).
A hard thin translucent layer of calcified substance which envelops and protects the dentin of the crown of the tooth. It is the hardest substance in the body and is almost entirely composed of calcium salts. Under the microscope, it is composed of thin rods (enamel prisms) held together by cementing substance, and surrounded by an enamel sheath. (From Jablonski, Dictionary of Dentistry, 1992, p286)
Cylindrical epithelial cells in the innermost layer of the ENAMEL ORGAN. Their functions include contribution to the development of the dentinoenamel junction by the deposition of a layer of the matrix, thus producing the foundation for the prisms (the structural units of the DENTAL ENAMEL), and production of the matrix for the enamel prisms and interprismatic substance. (From Jablonski's Dictionary of Dentistry, 1992)
An acquired or hereditary condition due to deficiency in the formation of tooth enamel (AMELOGENESIS). It is usually characterized by defective, thin, or malformed DENTAL ENAMEL. Risk factors for enamel hypoplasia include gene mutations, nutritional deficiencies, diseases, and environmental factors.
Spherical particles of nanometer dimensions.
A clinically and genetically heterogeneous group of hereditary conditions characterized by malformed DENTAL ENAMEL, usually involving DENTAL ENAMEL HYPOPLASIA and/or TOOTH HYPOMINERALIZATION.
The collective tissues from which an entire tooth is formed, including the DENTAL SAC; ENAMEL ORGAN; and DENTAL PAPILLA. (From Jablonski, Dictionary of Dentistry, 1992)
The process whereby calcium salts are deposited in the dental enamel. The process is normal in the development of bones and teeth. (Boucher's Clinical Dental Terminology, 4th ed, p43)
One of a set of bone-like structures in the mouth used for biting and chewing.
A group of phosphate minerals that includes ten mineral species and has the general formula X5(YO4)3Z, where X is usually calcium or lead, Y is phosphorus or arsenic, and Z is chlorine, fluorine, or OH-. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Epithelial cells surrounding the dental papilla and differentiated into three layers: the inner enamel epithelium, consisting of ameloblasts which eventually form the enamel, and the enamel pulp and external enamel epithelium, both of which atrophy and disappear before and upon eruption of the tooth, respectively.
Calcium salts of phosphoric acid. These compounds are frequently used as calcium supplements.
Large herbivorous tropical American lizards.
The most posterior teeth on either side of the jaw, totaling eight in the deciduous dentition (2 on each side, upper and lower), and usually 12 in the permanent dentition (three on each side, upper and lower). They are grinding teeth, having large crowns and broad chewing surfaces. (Jablonski, Dictionary of Dentistry, 1992, p821)
Validation of the SEX of an individual by inspection of the GONADS and/or by genetic tests.
Proteins obtained from species of REPTILES.
Any of the eight frontal teeth (four maxillary and four mandibular) having a sharp incisal edge for cutting food and a single root, which occurs in man both as a deciduous and a permanent tooth. (Jablonski, Dictionary of Dentistry, 1992, p820)
The mineral component of bones and teeth; it has been used therapeutically as a prosthetic aid and in the prevention and treatment of osteoporosis.
Nanometer-sized tubes composed of various substances including carbon (CARBON NANOTUBES), boron nitride, or nickel vanadate.
The process of TOOTH formation. It is divided into several stages including: the dental lamina stage, the bud stage, the cap stage, and the bell stage. Odontogenesis includes the production of tooth enamel (AMELOGENESIS), dentin (DENTINOGENESIS), and dental cementum (CEMENTOGENESIS).
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
Tissue, organ, or gamete donation intended for a designated recipient.
The mechanisms by which the SEX of an individual's GONADS are fixed.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
A spectroscopic technique in which a range of wavelengths is presented simultaneously with an interferometer and the spectrum is mathematically derived from the pattern thus obtained.
The physiological renewal, repair, or replacement of tissue.
A dental specialty concerned with the histology, physiology, and pathology of the tissues that support, attach, and surround the teeth, and of the treatment and prevention of disease affecting these tissues.
Techniques for enhancing and directing cell growth to repopulate specific parts of the PERIODONTIUM that have been damaged by PERIODONTAL DISEASES; TOOTH DISEASES; or TRAUMA, or to correct TOOTH ABNORMALITIES. Repopulation and repair is achieved by guiding the progenitor cells to reproduce in the desired location by blocking contact with surrounding tissue by use of membranes composed of synthetic or natural material that may include growth inducing factors as well.
The fibrous CONNECTIVE TISSUE surrounding the TOOTH ROOT, separating it from and attaching it to the alveolar bone (ALVEOLAR PROCESS).
Critical and exhaustive investigation or experimentation, having for its aim the discovery of new facts and their correct interpretation, the revision of accepted conclusions, theories, or laws in the light of newly discovered facts, or the practical application of such new or revised conclusions, theories, or laws. (Webster, 3d ed)
The formation of DENTAL CEMENTUM, a bone-like material that covers the root of the tooth.

Analysis of ancient DNA from a prehistoric Amerindian cemetery. (1/231)

The Norris Farms No. 36 cemetery in central Illinois has been the subject of considerable archaeological and genetic research. Both mitochondrial DNA (mtDNA) and nuclear DNA have been examined in this 700-year-old population. DNA preservation at the site was good, with about 70% of the samples producing mtDNA results and approximately 15% yielding nuclear DNA data. All four of the major Amerindian mtDNA haplogroups were found, in addition to a fifth haplogroup. Sequences of the first hypervariable region of the mtDNA control region revealed a high level of diversity in the Norris Farms population and confirmed that the fifth haplogroup associates with Mongolian sequences and hence is probably authentic. Other than a possible reduction in the number of rare mtDNA lineages in many populations, it does not appear as if European contact significantly altered patterns of Amerindian mtDNA variation, despite the large decrease in population size that occurred. For nuclear DNA analysis, a novel method for DNA-based sex identification that uses nucleotide differences between the X and Y copies of the amelogenin gene was developed and applied successfully in approximately 20 individuals. Despite the well-known problems of poor DNA preservation and the ever-present possibility of contamination with modern DNA, genetic analysis of the Norris Farms No. 36 population demonstrates that ancient DNA can be a fruitful source of new insights into prehistoric populations.  (+info)

Rapid quantification of mixed chimerism using multiplex amplification of short tandem repeat markers and fluorescence detection. (2/231)

Monitoring the engraftment of donor cells after allogeneic blood stem cell transplantation (BSCT) may be important for the early diagnosis of graft failure or relapse of disease. Several techniques have been reported for this purpose. PCR-based assays analyzing polymorphic short tandem repeat (STR) markers are attractive because they are sensitive and can be performed rapidly. The intent of the present study was to test a novel approach for the quantification of mixed chimerism using a commercial multiplex STR assay with fluorescence-based detection for forensic purposes. The feasibility of this assay and the accuracy of quantitative results was tested using serial cell mixtures of unrelated individuals. Sample preparation was optimized to obtain information from minute amounts of starting material, eg from patients with aplasia or from sorted cell populations. Using the STR-PCR, discrimination between donor and recipient was possible in all patients analyzed (n = 25). Cell dilution experiments showed a linear correlation between the cell numbers added and the proportions found, with the limit of detection for a minor cell population being 5%. Comparison of values obtained with standard FISH analysis in patients transplanted from sex-mismatched donors showed an excellent correlation with the STR-PCR results. Taken together, this procedure allows the rapid, versatile and accurate quantification of mixed chimerism, even with minuscule numbers of cells.  (+info)

An immunocytochemical study of amelogenin proteins in the developing tooth enamel of the gar-pike, Lepisosteus oculatus (Holostei, Actinopterygii). (3/231)

Previous studies have demonstrated the morphological similarity of the enamel-like layer found in the teeth of the coelacanth, lungfish and gar-pike to the enamel of tetrapods. In order to clarify the phylogenetic continuity between both structures, tooth germs of the gar-pike were immunocytochemically studied using an anti-bovine amelogenin polyclonal antibody. Intense immunoreaction was shown over the enamel-like matrix layer. Certain cell organelles associated with the secretory pathway of the ameloblasts were recognized as immunoreactive. These results indicate that the enamel-like layer of the gar-pike is a tissue homologous with the mammalian enamel because both possess a common, amelogenin-like substance.  (+info)

Histological analysis and ancient DNA amplification of human bone remains found in caius iulius polybius house in pompeii. (4/231)

Thirteen skeletons found in the Caius Iulius Polybius house, which has been the object of intensive study since its discovery in Pompeii 250 years ago, have provided an opportunity to study either bone diagenesis by histological investigation or ancient DNA by polymerase chain reaction analysis. DNA analysis was done by amplifying both X- and Y-chromosomes amelogenin loci and Y-specific alphoid repeat locus. The von Willebrand factor (vWF) microsatellite locus on chromosome 12 was also analyzed for personal identification in two individuals showing alleles with 10/11 and 12/12 TCTA repeats, respectively. Technical problems were the scarcity of DNA content from osteocytes, DNA molecule fragmentation, microbial contamination which change bone structure, contaminating human DNA which results from mishandling, and frequent presence of Taq DNA polymerase inhibiting molecules like polyphenols and heavy metals. The results suggest that the remains contain endogenous human DNA that can be amplified and analyzed. The amplifiability of DNA corresponds to the bone preservation and dynamics of the burial conditions subsequent to the 79 A.D. eruption.  (+info)

Proteinases in developing dental enamel. (5/231)

For almost three decades, proteinases have been known to reside within developing dental enamel. However, identification and characterization of these proteinases have been slow and difficult, because they are present in very small quantities and they are difficult to purify directly from the mineralizing enamel. Enamel matrix proteins such as amelogenin, ameloblastin, and enamelin are cleaved by proteinases soon after they are secreted, and their cleavage products accumulate in the deeper, more mature enamel layers, while the full-length proteins are observed only at the surface. These results suggest that proteinases are necessary for "activating" enamel proteins so the parent proteins and their cleavage products may perform different functions. A novel matrix metalloproteinase named enamelysin (MMP-20) was recently cloned from tooth tissues and was later shown to localize primarily within the most recently formed enamel. Furthermore, recombinant porcine enamelysin was demonstrated to cleave recombinant porcine amelogenin at virtually all of the sites that have previously been described in vivo. Therefore, enamelysin is at least one enzyme that may be important during early enamel development. As enamel development progresses to the later stages, a profound decrease in the enamel protein content is observed. Proteinases have traditionally been assumed to degrade the organic matrix prior to its removal from the enamel. Recently, a novel serine proteinase named enamel matrix serine proteinase-1 (EMSP1) was cloned from enamel organ epithelia. EMSP1 localizes primarily to the early maturation stage enamel and may, therefore, be involved in the degradation of proteins prior to their removal from the maturing enamel. Other, as yet unidentified, proteinases and proteinase inhibitors are almost certainly present within the forming enamel and await discovery.  (+info)

Identification of CCAAT/enhancer-binding protein alpha as a transactivator of the mouse amelogenin gene. (6/231)

Amelogenin expression is ameloblast-specific and developmentally regulated at the temporal and spatial levels. In a previous transgenic mouse analysis, the expression pattern of the endogenous amelogenin gene was recapitulated by a reporter gene driven by a 2. 2-kilobase mouse amelogenin proximal promoter. To understand the molecular mechanisms underlying the spatiotemporal expression of the amelogenin gene during odontogenesis, the mouse amelogenin promoter was systematically analyzed in mouse ameloblast-like LS8 cells. Deletion analysis identified a minimal promoter (-70/+52) containing a CCAAT/enhancer-binding protein (C/EBP)-binding site upstream of the TATA box. In transient transfection assays, C/EBPalpha up-regulated the promoter activity in a dose-dependent manner. The C/EBP-binding site was necessary for both C/EBPalpha-mediated transactivation and basal promoter activity. Electrophoresis mobility shift assays demonstrated that C/EBPalpha bound to its cognate site in the amelogenin promoter and that the binding was specific. Endogenous C/EBPalpha was detected in LS8 cells, and overexpression of exogenous C/EBPalpha in LS8 cells was able to increase the expression level of the endogenous amelogenin protein. The activity of the amelogenin promoter in rat parotid Pa-4 cells and Madin-Darby canine kidney cells was minimal, ranging from 20 to 30% of the activity in ameloblast-like cells. Transient transfection experiments showed that C/EBPalpha transactivated the mouse amelogenin reporter gene in Pa-4 cells, but not in Madin-Darby canine kidney cells. Taken together, these data indicate that C/EBPalpha is a bona fide transcriptional activator of the mouse amelogenin gene in a cell type-specific manner.  (+info)

Functional antagonism between Msx2 and CCAAT/enhancer-binding protein alpha in regulating the mouse amelogenin gene expression is mediated by protein-protein interaction. (7/231)

Ameloblast-specific amelogenin gene expression is spatiotemporally regulated during tooth development. In a previous study, the CCAAT/enhancer-binding protein alpha (C/EBPalpha) was identified as a transcriptional activator of the mouse amelogenin gene in a cell type-specific manner. Here, Msx2 is shown to repress the promoter activity of amelogenin-promoter reporter constructs independent of its intrinsic DNA binding activity. In transient cotransfection assays, Msx2 and C/EBPalpha antagonize each other in regulating the expression of the mouse amelogenin gene. Electrophoresis mobility shift assays demonstrate that Msx2 interferes with the binding of C/EBPalpha to its cognate site in the mouse amelogenin minimal promoter, although Msx2 itself does not bind to the same promoter fragment. Protein-protein interaction between Msx2 and C/EBPalpha is identified with co-immunoprecipitation analyses. Functional antagonism between Msx2 and C/EBPalpha is also observed on the stably transfected 2.2-kilobase mouse amelogenin promoter in ameloblast-like LS8 cells. Furthermore, the carboxyl-terminal residues 183-267 of Msx2 are required for protein-protein interaction, whereas the amino-terminal residues 2-97 of Msx2 play a less critical role. Among three family members tested (C/EBPalpha, -beta, and -gamma), Msx2 preferentially interacts with C/EBPalpha. Taken together, these data indicate that protein-protein interaction rather than competition for overlapping binding sites results in the functional antagonism between Msx2 and C/EBPalpha in regulating the mouse amelogenin gene expression.  (+info)

Time-related changes of developing enamel crystals after exposure to the tissue fluid in vivo: analysis of a subcutaneously implanted rat incisor. (8/231)

To investigate the effects of tissue fluid on the growth of enamel crystals, upper and lower incisors extracted from 3-week-old Wistar rats were removed of the enamel organ, implanted subcutaneously in the dorsal portion of animals from the same litter, and harvested at 72 h or 1 week after implantation. The grafts were chemically fixed with surrounding tissues and prepared for light and electron microscopy, X-ray microanalysis, or for the immunohistochemistry of amelogenin. Mineralization of implanted enamel layers was examined by contact X-ray microradiography. The immunoreactivities for 25 kD amelogenin in immature enamel decreased sequentially, starting from the surface to the deeper layers; by 1 week after implantation, no positive reactivities remained in the entire enamel layers at the stages of matrix formation and early maturation. In accordance with the loss of enamel proteins, immature enamel gained mineral density until it attained higher radio opacity than that of the adjacent dentin by 1 week. In contrast, the radio opacity of the full thickness of the enamel at early maturation remained low except for a superficial thin layer. Electron microscopy revealed no sign of growth of original enamel crystals, but showed heavy precipitation of electron-dense fine granules of calcium phosphate in all layers of the secretory enamel and the superficial layer of enamel at early maturation, which showed high radio opacity. The Ca/P ratio and electron diffraction patterns of the granular materials precipitated between intrinsic enamel crystals indicated the property of hydroxy apatite or octacalcium phosphate though a characteristic ribbon-like profile of enamel crystals was lacking. These data indicate that the enamel organ blocks exogenous mineral precipitates in growing enamel during the stage of matrix formation and plays an essential regulatory role for fine enamel crystallites to grow into large hexagonal crystals.  (+info)

Previously, we have shown that serine-16 phosphorylation in native full-length porcine amelogenin (P173) and the Leucine-Rich Amelogenin Peptide (LRAP(+P)), an alternative amelogenin splice product, affects protein assembly and mineralization in vitro. Notably, P173 and LRAP(+P) stabilize amorphous calcium phosphate (ACP) and inhibit hydroxyapatite (HA) formation, while non-phosphorylated counterparts (rP172, LRAP(−P)) guide the growth of ordered bundles of HA crystals. Based on these findings, we hypothesize that the phosphorylation of full-length amelogenin and LRAP induces conformational changes that critically affect its capacity to interact with forming calcium phosphate mineral phases. To test this hypothesis, we have utilized Fourier transform infrared spectroscopy (FTIR) to determine the secondary structure of LRAP(−P) and LRAP(+P) in the absence/presence of calcium and selected mineral phases relevant to amelogenesis; i.e., hydroxyapatite (HA: an enamel crystal prototype) and (ACP: an
Amelogenin gene expression is spatiotemporally regulated during enamel biomineralization. Studies show that C/EBP alpha is a transactivator of the mouse amelogenin gene acting at the C/EBP alpha cis-element located in the -70/+52 minimal promoter that also contains a reversed CCAAT box (-58/-54). Similar to the C/EBP alpha binding site, this CCAAT box is required for the basal promoter activity. Electrophoretic mobility shift assays demonstrate that NF-Y is directly bound to this reversed CCAAT box. Co-transfection of C/EBP alpha and NF-Y synergistically increases the promoter activity. Protein-protein interactions between C/EBP alpha with NF-Y are identified by a co-immunoprecipitation analysis.; A protein/DNA array technique is utilized to identify a transcriptional factor named YY1. YY1 represses both the basal amelogenin promoter activity and C/EBP alpha-mediated transactivation. Furthermore, YY1 repression is independent of its DNA binding capacity.; C/EBP alpha contains four highly ...
Differences between the X chromosome and Y chromosome versions of the amelogenin gene (AMELX and AMELY respectively) enable it to be used in sex determination of unknown human samples. AMELXs intron 1 contains a 6 bp deletion relative to intron 1 of AMELY. This can be detected at low cost using Polymerase Chain Reaction (PCR) of intron 1, followed by gel electrophoresis. Two bands of DNA, at 106bps and 112bps, are resolved if both the AMELX and AMELY versions of the gene are present (i.e. the sample is from a male) or one band of DNA, at 106bps, if the AMELX version only is present (i.e. the sample is from a female).[3]. However because of AMELY variation among individuals and populations, this method of sex determination is not 100% accurate. Mutation in regions of AMELY intron 1 commonly used as primer annealing sites may disable PCR amplification. A 6bp insertion to AMELY intron 1 results in an amplicon identical in length to that of AMELX. In some males AMELY may be deleted entirely. In any ...
An unresolved problem in tooth enamel studies has been to analyze simultaneously and with sufficient spatial resolution both mineral and organic phases in their three dimensional (3D) organization in a given specimen. This study aims to address this need using high-resolution imaging to analyze the 3D structural organization of the enamel matrix, especially amelogenin, in relation to forming enamel crystals. Chemically fixed hemi-mandibles from wild type mice were embedded in LR White acrylic resin, polished and briefly etched to expose the organic matrix in developing tooth enamel. Full-length amelogenin was labeled with specific antibodies and 10 nm immuno-gold. This allowed us to use and compare two different high-resolution imaging techniques for the analysis of uncoated samples. Helium ion microscopy (HIM) was applied to study the spatial organization of organic and mineral structures, while field emission scanning electron microscopy (FE-SEM) in various modes, including backscattered electron
Recent work has disclosed the critical role played by enamel peptides in sex classification of old skeletal remains. In particular, protein AMELY (amelogenin isoform Y) is present in the enamel dental tissue of male individuals only, while AMELX (isoform X) can be found in both sexes. AMELY can be easily detected by LC-MS/MS in the ion extracted chromatograms of the SM(ox)IRPPY peptide (monoisotopic [M + 2 H]+2 mass = 440.2233 m/z). In this paper, we exploited the dimorphic features of the amelogenin protein to determine the sex of the so-called Lovers of Modena, two Late Antique individuals whose skeletons were intentionally buried hand-in-hand. Upon discovery, mass media had immediately assumed they were a male-female couple, even if bad preservation of the bones did not allow an effective sex classification. We were able to extract proteins from the dental enamel of both individuals (~1600 years old) and to confidently classify them as males. Results were compared to 14 modern and archaeological
This volume is the 1st in a series of Ebooks that bridges the gap between advances in science and clinical practice in odontology. Recent advances in biology, materials science and tissue engineering are increasingly viewed as being of enormous clinical potential. Stem cell research has opened up the possibility of reconstructing teeth from the association of epithelial and mesanchymal embryonic or adult cells, as an exciting alternative to metal implants. This Ebook will examine the multifunctional nature of a group of proteins known as the amelogenins. Latest studies indicate that this protein regulates the initiation and growth of hydroxyapatite crystals during the mineralization of enamel. In addition, amelogenins organize enamel rods during tooth development, and also aid in the development of cementum by directing cells that form the cementum to the root surface of the teeth. The aim of this book is to serve as a bridge between basic biology and biomaterial sciences, and to inform ...
Sure, there could be autosomal marker that influences male/female outcome but there is a more likely explanation: All SNPs have paralogue sequence stretches on the Y chromosome that are co-amplified during PCR. From the initial genotyping results it is most likely that only the Y chromosomal stretch is being mutated in SNP 4, 13 and 15.2. These SNPs are perfect sex marker, as they include an autosomal control allele (in comparison to pure Y markers like SNPs in SRY). They are always unambiguous (in contrast to pure X marker where only heterocygotes are informative).. They even offer advantage to commercial STR kits of the Amelogenin/Amely gene situated (in the Y parautosomal region) as they would not be affected by excess homologous X chromosomal material as often found in forensic situations. In addition, they might overcome some other weakness of the Amelogenin test where a second assay is usually recommended. If you will ever see a case-control study that is highlighting any of these SNPs, ...
For direct STR multiplex amplification of the CODIS and ESS loci, SE33, DYS391 and Amelogenin, with an innovative Quality Sensor ...
For direct STR multiplex amplification of the CODIS and ESS loci, plus SE33, DYS391 and Amelogenin, with an innovative Quality Sensor
Lyrics to Make Me Whole by Amel Larrieux: Darlin I want you to listen, / I stayed up all night, / So I can get this thing right. / And I
TY - JOUR. T1 - The influence of Leucine-rich amelogenin peptide on MSC fate by inducing Wnt10b expression. AU - Wen, Xin. AU - Cawthorn, William P.. AU - MacDougald, Ormond A.. AU - Stupp, Samuel I.. AU - Snead, Malcolm L.. AU - Zhou, Yan. PY - 2011/9/1. Y1 - 2011/9/1. N2 - Amelogenin is the most abundant protein of the enamel organic matrix and is a structural protein indispensable for enamel formation. One of the amelogenin splicing isoforms, Leucine-rich Amelogenin Peptide (LRAP) induces osteogenesis in various cell types. Previously, we demonstrated that LRAP activates the canonical Wnt signaling pathway to induce osteogenic differentiation of mouse ES cells through the concerted regulation of Wnt agonists and antagonists. There is a reciprocal relationship between osteogenic and adipogenic differentiation in bone marrow mesenchymal stem cells (BMMSCs). Wnt10b-mediated activation of canonical Wnt signaling has been shown to regulate mesenchymal stem cell fate. Using the bipotential bone ...
leucine-rich amelogenin peptide: translation product of an alternatively spliced transcript; amino acid sequence has been determined
Masica, David L., and Rachel Karchin. Collections of simultaneously altered genes as biomarkers of cancer cell drug response. Cancer research 73, no. 6 (2013): 1699-1708.. Masica, David L., Patrick R. Sosnay, Garry R. Cutting, and Rachel Karchin. Phenotype-optimized sequence ensembles substantially improve prediction of disease-causing mutation in cystic fibrosis. Human Mutation (2012).. Masica, David L., and Rachel Karchin. Correlation of somatic mutation and expression identifies genes important in human glioblastoma progression and survival. Cancer research 71, no. 13 (2011): 4550-4561.. Masica, David L., Jeffrey J. Gray, and Wendy J. Shaw. Partial high-resolution structure of phosphorylated and non-phosphorylated leucine-rich amelogenin protein adsorbed to hydroxyapatite. The Journal of Physical Chemistry C 115, no. 28 (2011): 13775-13785.. Masica, David L., Sarah B. Schrier, Elizabeth A. Specht, and Jeffrey J. Gray. De Novo Design of Peptide-Calcite Biomineralization Systems. ...
As a quality control measure the amelogenin sex gene is tested as part of the paternity test. The gene confirms the sex declared by the applicant and can help deter fraud.
Principal Investigator:Toyoda Kyosuke, Project Period (FY):2015-08-28 - 2017-03-31, Research Category:Grant-in-Aid for Research Activity start-up, Research Field:Periodontology
The bone matrix and tooth enamel are the hardest substances in the body, and both are formed through the mineralization of proteins secreted by osteoblasts and ameloblasts, respectively. One of the primary components of the bone matrix is collagen, which provides strength, as well as acts as a substrate for mineralization. Amelogenin is the main extracellular matrix protein secreted by ameloblasts and is important for both the inclusion of minerals and the proper structure of tooth enamel. Defects in the secretion or structure of these extracellular matrices compromise the strength and durability of the tissues. Two studies reveal distinct mechanisms of secretory dysregulation that affect proper mineralization of these tissues. Zhao et al. found that the trimeric intracellular cation channel Tric-b, which localizes to the endoplasmic reticulum (ER), was important to secretion of collagen by osteoblasts in mice. Mutations in TMEM38b, the human homolog of Tric-b, are associated with osteogenesis ...
Effectss of EMDs on the epithelial tissue. Two surveies by Gestrelius et Al ( 1997b ) and Kawase et Al. ( 2000 ) examined the proliferation of unwritten epithelial cells utilizing in vitro rat lingua epithelial cells and in vivo trials severally. Both concluded that EMDs did non heighten proliferation of epithelial cells ; instead they arrested the growing of the cells, by maintaining them locked on the G1 stage of the mitotic rhythm. There has been grounds that the TGF-i?? may be the factor that causes this suppression. ( Bosshardt 2008 ). However, it has been witnessed by Rincon et Al. ( 2005 ) , that EMD significantly increases DNA synthesis from a specialized epithelial cell type called the Remainders of Malassez. These are the remnant cells of what originally used to be portion of the HERS during development that have since migrated into the periodontic infinite. Their map remains mostly unknown, nevertheless it has been proposed that their map may be. linked to regeneration, as they react ...
Aichmayer, B.; Margolis, H. C.; Sigel, R.; Yamakoshi, Y.; Simmer, J. P.; Fratzl, P.: The onset of amelogenin nanosphere aggregation studied by small-angle X-ray scattering and dynamic light scattering. Journal of Structural Biology 151 (3), S. 239 - 249 (2005 ...
FUNCTION: This gene encodes a member of the kallikrein subfamily of serine proteases that are involved in diverse physiological functions such as skin desquamation, tooth enamel formation, seminal liquefaction, synaptic neural plasticity and brain function. The encoded preproprotein undergoes proteolytic cleavage of the activation peptide to generate the functional enzyme. This gene is located in a cluster of several related kallikrein genes on chromosome 7. [provided by RefSeq, Feb 2016 ...
Article Multi-component supramolecular assembly structures studied by scanning tunnelling microscopy. In this review, recent progress on the multi-component self-assembled structure and assembling mechanism are discussed. The effects of various inter...
Page contains details about porphyrin supramolecular assemblies . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
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Dental enamel is the hardest mineralized tissue in the body and initially forms from a protein matrix. During enamel formation, ameloblasts secrete unique proteins into the extracellular space to direct mineralization of enamel crystallites. This specialized organic matrix contains the proteins amelogenin, ameloblastin, and enamelin. Amelogenin is the major enamel matrix protein secreted, comprising ~95% of the total matrix, yet after maturation and completion of amelogenesis, only ~1% of the initial organic matrix remains in mineralized enamel. Ameloblasts, being the only cells in close proximity to the organic matrix, most likely are responsible for the removal of proteinaceous matrix debris for proper enamel maturation. The rapid removal of enamel matrix proteins is poorly understood in the literature, principally because clathrin (the prototypical pathway for endocytosis) is expressed at very low levels in ameloblasts. Most of the historical record of endocytosis is focused on clathrin ...
Amelotin (AMTN) is an ameloblast-secreted protein that belongs to the secretory calcium-binding phosphoprotein (SCPP) family, which originated in early vertebrates. In rodents, AMTN is expressed during the maturation stage of amelogenesis only. This expression pattern strongly differs from the spatiotemporal expression of other ameloblast-secreted SCPPs, such as the enamel matrix proteins (EMPs). Furthermore, AMTN was characterized in rodents only. In this study, we applied various approaches, including in silico screening of databases, PCRs and transcriptome sequencing to characterize AMTN sequences in sauropsids and amphibians, and compared them to available mammalian and coelacanth sequences. We showed that (i) AMTN is tooth (enamel) specific and underwent pseudogenization in toothless turtles and birds, and (ii) the AMTN structure changed during tetrapod evolution. To infer AMTN function, we studied spatiotemporal expression of AMTN during amelogenesis in a salamander and a lizard, and compared the
TY - JOUR. T1 - Identification of the chondrogenic-inducing activity from bovine dentin (bCIA) as a low-molecular-mass amelogenin polypeptide. AU - Nebgen, D. R.. AU - Inoue, H.. AU - Sabsay, B.. AU - Wei, K.. AU - Ho, C. S.. AU - Veis, A.. PY - 1999. Y1 - 1999. N2 - Dentin extracellular matrix has been shown to contain components capable of inducing chondrogenesis and osteogenesis at ectopic sites when implanted in vivo, and chondrogenesis in cultures of embryonic muscle-derived fibroblasts (EMF) in vitro. The polypeptide responsible, called the chondrogenic-inducing agent (CIA), has been isolated from a 4.0-M guanidinium hydrochloride extract of demineralized bovine dentin matrix. Following Sephacryl S-100 chromatography, CIA activity was identified in fractions by assay for uptake of [35S]-SO4 into proteoglycan by the EMF after 24 hrs in culture. The active fraction induced the EMF to produce type II collagen mRNA and decrease production of type I collagen mRNA after 5 days in culture. The ...
The conversion of bioactive glass to a calcium phosphate material, typically hydroxyapatite (HA), by solution-precipitation reactions in aqueous phosphate solution, has been commonly reported. This paper describes the structural and compositional characteristics of the calcium phosphate material formed during the early-stage conversion (o5 h) of a borosilicate glass (designated H12) in aqueous phosphate solution. Disks of H12 glass were reacted with 0.25M K2HPO4 solution with a starting pH57.0 at 371C. The structure and composition of the product layer were characterized using thin film X-ray diffraction, scanning electron microscopy, and Fourier transform infrared reflectance spectroscopy. For reaction times o5 h, the results indicated the formation of brushite (CaHPO4 .2H2O) with coarse, plate-like crystals. In comparison, the formation of HA with small needle-like crystals was found at later times. This early-stage formation of brushite has not been reported in previous studies of converting
The flexible structure of polymers has enabled them to be useful in a wide variety of medical applications due to the possibility to tailor their properties to suit desired applications. For a long ti
In Turner syndrome (TS) one X-chromosome is missing or defective. The amelogenin gene, located on the X-chromosome, plays a key role during the formation of dental enamel. The aim of this study was to find support for the hypothesis that impaired expression of the X-chromosome influences mineral incorporation during amelogenesis and, indirectly, during dentinogenesis. Primary tooth enamel and dentin from girls with TS were analysed and compared with the enamel and dentin of primary teeth from healthy girls. Qualitative and quantitative changes in the composition of TS enamel were found, in addition to morphological differences. Higher frequencies of subsurface lesions and rod-free zones were seen in TS enamel using polarized light microscopy. Similarly, scanning electron microscopy showed that the enamel rods from TS teeth were of atypical sizes and directions. Using X-ray microanalysis, high levels of calcium and phosphorus, and low levels of carbon, were found in both TS enamel and dentin. ...
In the framework of the project Immigration and mobility in mediaeval and post-mediaeval Norway molecular genetic analyses were performed on 97 pre-modern human remains including genetic sexing and Y-chromosomal DNA typing. All samples were subjected to molecular genetic analyses of the sex using Genderplex consisting of two diff erent regions of the amelogenin gene, SRY and four X-STR loci. From 90% of the extracted remains (n=87) sex assignment was possible. Of these, 49 (56.3%) brought a genetically male result. All of these DNA extracts were subjected to Y-STR analysis using Yfiler Plus PCR Amplification Kit (Thermo Fisher Scientifi c) and/or PowerPlex Y23 System (Promega). At least partial Y-STR profiles were obtained from all samples. A detailed comparison between mediaeval/post-mediaeval and contemporary Y-chromosomes was performed by searching the obtained haplotypes (HTs) in the Y Chromosome Haplotype Reference Database (YHRD: https://yhrd.org) comprising 154,329 haplotypes from 991 ...
A supramolecular assembly or supermolecule is a well defined complex of molecules held together by noncovalent bonds. While a supramolecular assembly can be simply composed of two molecules (e.g., a DNA double helix or an inclusion compound), it is more often used to denote larger complexes of molecules that form sphere-, rod-, or sheet-like species. Micelles, liposomes and biological membranes are examples of supramolecular assemblies. The dimensions of supramolecular assemblies can range from nanometers to micrometers. Thus they allow access to nanoscale objects using a bottom-up approach in far fewer steps than a single molecule of similar dimensions. The process by which a supramolecular assembly forms is called molecular self-assembly. Some try to distinguish self-assembly as the process by which individual molecules form the defined aggregate. Self-organization, then, is the process by which those aggregates create higher-order structures. This can become useful when talking about liquid ...
The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format. By default, clicking on the export buttons will result in a download of the allowed maximum amount of items. To select a subset of the search results, click Selective Export button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export. After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format. ...
Loratadine Amel is a medicine available in a number of countries worldwide. A list of US medications equivalent to Loratadine Amel is available on the Drugs.com website.
Supporting: 4, Mentioning: 2 - Influence of molecular structures of europium bisphthalocyanines on organization of supramolecular assemblies formed at the air/water interface - Hong-Guo Liu, Xu-Sheng Feng, Jianzhuang Jiang, Li-Juan Zhang, Wen-Zhong Lan, Yong-Ill Lee, Kiwan Jang, Dong-Jin Qian, Kong-Zhang Yang
akkor elker lhetetlenek a tov bbi bonyodalmak. Mi rt? A negat v el jel B m trixok, amelyek az tl val p rhuzamos k vetkez diagon lisban llnak, a k vetkez v sz ks ges r ford t sai. M sk ppen nem volna el gs ges kapacit s a sz ks ges tev kenys gek elv gz s re. Ezekkel szemben llnak a pozit v el jel B m trixok a f tl ban. A pozit v el jel azt jelenti, hogy ezek az adott vek kibocs t sai. A k tszeres szerepeltet s elm letileg nem kifog solhat . Neumann J nos tal lta ki, hogy gy lehet br zolni azt az ll t k t, amely megmarad a termel si folyamatban, t bb id szakaszra terjed lettartama alatt. Sraffa s az gynevezett Economic Activity Analysis m vel i is kihaszn lt k a matematikai t rgyal snak ezt az elm leti lehet s g t a t ke korrekt br zol s ra. Ez a matematikai le r s a t k t saj tos ikerterm knek tekinti, amely csak abban k l nb zik az egy b term kekt l, hogy eredeti hely n maradva ker l vissza a termel si folyamatba. Ez esetben, mint egy egy v term k t, ssze szabad vonni az adott v foly r ford t ...
A diffúz nagy B-sejtes lymphoma (DLBCL) a leggyakoribb malignus lymphomatípus, amely a non-Hodgkin-lymphomák (NHL) 31%-át teszi ki. A standard kemoterápiás protokollok jelentős toxicitásuk miatt nem vagy nehezen alkalmazhatóak az idősebb betegpopulációban, különösen a relabált esetekben, ahol kuratív lehetőséget leginkább a nagy dózisú kezelés és a vér-őssejtátültetés jelenthetne. Egyre több adat lát napvilágot a thalidomid és az újabb IMiD-ek (lenalidomid, pomalidomid) kedvező hatásairól az NHL kezelésében, amelyek részei a standard kezelésnek myeloma multiplexben és myelodysplasiás szindrómában. Az IMiD-ek kiterjedt anti-angiogenetikus, immunmoduláns és gyulladásellenes hatásuknak köszönhetik az egyre széleskörűbb alkalmazást. 2010-ben azonosították az E3-ubiquitin-ligáz-komplex egyik komponensét, a cereblont, amely a thalidomid molekuláris támadási pontja. Az irodalomban közölt eredmények alapján thalidomidkezelést ...
Szeretném, ha ezen a lapon összegyűlnének annak a kultúrának az emlékei és élő tanúi, amely 250 éven át virágzott itt a Budai-hegyvidék szívében. Képek, hagyományok, videók, hírek, leírások, viseletek, táncok, személyes történetek, diplomamunkák és minden olyan elektronikus anyagot szívesen várunk, amely emléket állít és hagyományt ápol vagy csak egy személyes, kedves élmény Zsámbékról, zsámbékiakról.
Az ókori görög szövegekben találunk néhány olyan példát, amelyeket mai szóhasználattal élve „állatvédő törvényeknek nevezhetünk. Ezek a törvények vagy tiltják az állatok (illetve bizonyos állatok) megölését, vagy pedig szigorúan büntetik azokat, akik kárt vagy fájdalmat okoztak az állatoknak. Bár a példák meglehetősen elszórtan jelennek meg a görög és a latin irodalom egészében, mégis figyelemre méltóak. Egyesek közülük anekdotikus jelleggel bírnak, ez azonban nem lehet ok arra, hogy ne vegyük komolyan őket, amikor megkíséreljük felkutatni az állatokkal szembeni igazságos és kíméletes bánásmód nyomait az ókori görög felfogásban. Határozottan megtalálhatók egy olyan ősi kultikus törvényhozás nyomai, amely igazságos bánásmódot követelt meg a szelíd háziállatokkal szemben, amelyeknek az ember kulturált és civilizált életét köszönheti. Ez a törvényhozás két kultúrhérós, az eleusisi Triptolemos és az ...
A Pécsi Riport tartalmaz beszámolókat a város kulturális, közéleti, sport és minden olyan történéséről, amely Pécs város polgárait érdekelheti. Napilapunk megbízható forrásokból szerzi információit, melyet egy-egy esemény után szinte azonnal olvashat, képekkel és videóval.
A Pécsi Riport tartalmaz beszámolókat a város kulturális, közéleti, sport és minden olyan történéséről, amely Pécs város polgárait érdekelheti. Napilapunk megbízható forrásokból szerzi információit, melyet egy-egy esemény után szinte azonnal olvashat, képekkel és videóval.
Ny jtott felsz v d s filmtabletta, gy folyamatosan l tja el a szervezetet tablett nk nt 1200 mg C-vitaminnal, amely hozz j rul az immunrendszer s az idegrendszer norm l m k d s hez. Antioxid nsk nt t mogatja a sejtek oxidat v stresszel szembeni v delm t, a f radts g s kif rad s cs kkent s t, fokozza a vas felsz v d s t. T mogatja a kollag nk pz d st, a csontozat, a porcok, a b r, az erek, a fogak s a fog ny norm l llapot nak fenntart s t ...
Page contains details about ITO/VB-FNPD/mixed nanospheres/TPBi/LiF/Al . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
Suranjith Luke Seneviratne, Rainer Doffinger, John Macfarlane, L Ceron-Gutierrez, M R Amel Kashipaz, A Robbins, T Patel, P T Powell, D S Kumararatne, Richard J Powell ...
Fedail, Moneira Abd Allah Mansour; Supervisor,- Ahmed Ibrahim Shomo;Co-supervisor,- Amel Omer Bakhiet;Co-supervisor;- Magdi Salih Mansour (Sudan University of Science and Technology, 2010-01-01) ...
Tunisijas un Francijas valstspiederība, dzimis Le Petit Quevilly (76)1971. gada 6. aprīlī (vai 16. aprīlī saskaņā ar viņa Tunisijas identitātes karti); 1932. gada 9. februārī dzimušā Tijani BEN ALI un 1936. gada 23. februārī dzimušās Paulette HAZET (vai HAZAT) dēls, precējies ar Amel SAIED (vai SAID), uzņēmējsabiedrības vadītājs, adrese: Chouket El Arressa, Hammam - Sousse saskaņā ar viņa Tunisijas NIK Nr. 00297112, un adrese: 14, esplanade des Guinandiers à Bailly Romainvilliers (77) saskaņā ar viņa Francijas NIK Nr. 111277501841.. ...
Learn more about In His Own Words: Living With Coronary Artery Disease at Reston Hospital Center Bill, 72, never suspected he had a heart problem, until he suffered...
Amelogenin • American Academy of Cosmetic Dentistry • American Academy of Periodontology • American Association of Endodontists ...
... is located on the Y chromosome and encodes a form of amelogenin. Amelogenin is an extracellular matrix protein involved ... "Entrez Gene: amelogenin". Human AMELY genome location and AMELY gene details page in the UCSC Genome Browser. Nakahori Y, ... 1992). "The human enamel protein gene amelogenin is expressed from both the X and the Y chromosomes". Am. J. Hum. Genet. 50 (2 ... Amelogenin, Y isoform is a protein that in humans is encoded by the AMELY gene. ...
... s are cells which secrete the enamel proteins enamelin and amelogenin which will later mineralize to form enamel, the ... "Localization and quantitative co-localization of enamelin with amelogenin". Journal of Structural Biology. 183 (2): 239-49. doi ...
Markers commonly used include amelogenin, SRY and ZFX/ZFY. Various techniques have been developed using simple polymerase chain ...
It is part of the non-amelogenins, which comprise 10% of the total enamel matrix proteins. It is one of the key proteins ... All other EMPs are derived from enamelin, such as amelogenin. EMPs belong to a larger family of proteins termed 'secretory ... Ameloblastin Amelogenin Amelogenesis Amelogenesis imperfecta GRCh38: Ensembl release 89: ENSG00000132464 - Ensembl, May 2017 ... such as amelogenin, to template calcium phosphate nucleation. It is best thought to understand the overarching function of ...
SGM Plus also uses the amelogenin (amelo) sex-indicating test. SGM Plus differs from SGM in that SGM does not use the markers ...
His other recent work is on self-assembly of amelogenin bio-macromolecules. Amelogenin is involved in the mineral deposition ... "Kinetics of Nanochain Formation in a Simplified Model of Amelogenin Biomacromolecules". Biophysical Journal. Elsevier BV. 101 ( ...
A commercially prepared and purified extract of enamel matrix proteins, EMD is composed primarily of amelogenin and has been ... Biochemistry and molecular biology of amelogenin proteins of developing dental enamel. Arch Oral Bio 1995;40:1-14 Boyan, BD, et ...
Amelogenin is used for a rapid test of a donor's sex. Scotland has used 21 STR loci, two Y-DNA markers and the gender ...
Enamel defects in coeliac disease occur due to an interference in tooth formation by amelogenin. Amelogenin is a proline-rich ... However, due to the structural similarities between amelogenin and gliadin, AGA may interfere with amelogenin which lead to ... Moradian-Oldak, Janet (2001-09-01). "Amelogenins: assembly, processing and control of crystal morphology". Matrix Biology. 20 ( ...
Other significant proteins in enamel are amelogenins, enamelins, and ameloblastins. The human encoding gene for tuftelin (TUFT1 ... There are two kinds of enamel proteins: Amelogenins & Nonamelogenins. Tuftelin falls under nonamelogenins. This protein is ...
... tuftelin and amelogenin) as a result of abnormal enamel formation via amelogenesis. People afflicted with amelogenesis ... "A deletion in the amelogenin gene (AMG) causes X-linked amelogenesis imperfecta (AIH1)". Genomics. 10 (4): 971-5. doi:10.1016/ ...
Other significant proteins in enamel are amelogenins, enamelins, and tuftelins. This gene encodes the nonamelogenin enamel ...
During this process, amelogenins and ameloblastins are removed after use, leaving enamelins and tuftelin in the enamel. By the ... Researchers at the University of Southern California have developed an amelogenin-chitosan peptide hydrogel which entraps ... The noteworthy proteins involved are amelogenins, ameloblastins, enamelins, and tuftelins. How these proteins are secreted into ... amelogenins and enamelins. While the role of these proteins is not fully understood, it is believed that they aid in the ...
The important proteins involved are amelogenins, ameloblastins, enamelins, and tuftelins. By the end of this stage, the enamel ...
Salido EC, Yen PH, Koprivnikar K, Yu LC, Shapiro LJ (February 1992). "The human enamel protein gene amelogenin is expressed ...
Proteins of note in the development of enamel are ameloblastins, amelogenins, enamelins and tuftelins. It is believed that they ...
... indeed contains amelogenin-like proteins and has a mineral content similar to that of tetrapod tooth enamel. Zylberberg ... L.; Sire, J. -Y.; Nanci, A. (1997). "Immunodetection of amelogenin-like proteins in the ganoine of experimentally regenerating ...
In enamel, the matrix for HA is formed by amelogenins and enamelins instead of collagen. Hydroxyapatite deposits in tendons ...
Zylberberg, L.; Sire, J. -Y.; Nanci, A. (1997). "Immunodetection of amelogenin-like proteins in the ganoine of experimentally ...
The DNA profiles in the NFDD are generated by analysing 15 different STR loci plus Amelogenin for sex determination. Different ... plus Amelogenin for sex determination. There were approximately 80,000 DNA profiles on the DCID in 2007, with the number ...
"Forensic application of a rapid and quantitative DNA sex test by amplification of the X-Y homologous gene amelogenin". ...
As the prisms in the enamel grow in thickness but not length, proteins (amelogenins and most non-amelogenins) are removed from ... As enamel is secreted, some mineralisation occurs by Ca2+ deposition between nanospheres of amelogenins forming crystallites. ... importantly amelogenins, ameloblastins, enamelins, and tuftelins. The Ca2+ mainly comes from the enamel organ, and not the ...
One is using fragments known to support natural mineralization proteins, such as Amelogenin, Collagen, or Dentin Phosphophoryn ... Proteins of the developing enamel extracellular matrix (such as Amelogenin) control initial mineral deposition (nucleation) and ...
Also contains primers for the Amelogenin sex indicating test. The primers are tagged with the following fluorescent dyes for ...
... interacts with amelogenin and proteins involved in exocytosis and vesicular trafficking, suggesting that these proteins ... and colocalize in these cells with amelogenin, the main component of enamel, encoded by the AMELX gene, which has been already ...
... and amelogenin (AMEL), had not yet taken place in Aetiocetus. For the most part, Aetiocetus retains a primitive tooth count of ...
... amelogenin) are enzymatically broken down and the rate at which the subsequent breakdown products are removed. Fluoride may ...
Although the precise role of amelogenin(s) in regulating the mineralization process is unknown, it is known that amelogenins ... "deleted-amelogenin males" (DAMs). In response the authors suggested that while the amelogenin sex test may be accurate in ... The amelogenin gene has been most widely studied in humans, where it is a single copy gene, located on the X and Y chromosomes ... Amelogenin is the name for a series of closely related proteins involved in amelogenesis, the development of enamel.[1] They ...
amelogenin, X isoform. Names. amelogenin (X chromosome, amelogenesis imperfecta 1). amelogenin (amelogenesis imperfecta 1, X- ... Full-length amelogenin may have a negative mitogenic impact on human dental pulp stem cells. Title: Full-length amelogenin ... Amelogenin; Amelogenins, cell adhesion proteins, play a role in the biomineralisation of teeth. ... Amelogenin; Amelogenins, cell adhesion proteins, play a role in the biomineralisation of teeth. ...
Compare amelogenin, X-linked ELISA Kits from leading suppliers on Biocompare. View specifications, prices, citations, reviews, ... amelogenin, X-linked ELISA Kits. The ELISA (enzyme-linked immunosorbent assay) is a widely used application for detecting and ... Your search returned 43 amelogenin, X-linked ELISA ELISA Kit across 10 suppliers. ...
Considering the importance of amelogenin protein in dental enamel formation, its ability to control apatite mineralization in ... Amelogenin and enamel biomimetics Q. Ruan and J. Moradian-Oldak, J. Mater. Chem. B, 2015, 3, 3112 DOI: 10.1039/C5TB00163C ... amelogenin and enamel biomimetics. We review the most recent findings on amelogenin secondary and tertiary structural ... Considering the importance of amelogenin protein in dental enamel formation, its ability to control apatite mineralization in ...
Amelogenin dosage compensation in carcinoma of colon, lung, liver and kidney, is not a marker of clonality in males. Zvejnieks ... Amelogenin p.M1T and p.W4S mutations underlying hypoplastic X-linked amelogenesis imperfecta. Kim, J.W., Simmer, J.P., Hu, Y.Y ... The human enamel protein gene amelogenin is expressed from both the X and the Y chromosomes. Salido, E.C., Yen, P.H., ... Amelogenin cross-amplification in the family Bovidae and its application for sex determination. Weikard, R., Pitra, C., Kühn, C ...
Pig Amelogenin Gene Expresses a Unique Exon 4 HU C.-C. , RYU O. H. , YAMAKOSHI Y. , ZHANG C. H. , CAO X. , QIAN Q. , SIMMER J. ... Porcine Amelogenin is Expressed from the X and Y Chromosomes IKAWA T. , KAKEGAWA A. , NAGANO T. , ANDO H. , YAMAKOSHI Y. , ... Micelle Structure of Amelogenin in Porcine Secretory Enamel FUKAE M. , YAMAMOTO R. , KARAKIDA T. , SHIMODA S. , TANABE T. ... Porcine Amelogenin : Alternative Splicing, Proteolytic Processing, Protein-Protein Interactions, and Possible Functions * * ...
Amelogenin (AMELX) with its splice variants is the most abundant enamel matrix protein, with only one known phosphorylation ... While AMELX regulates mineral transitions over space and time, it is unknown whether and when un-phosphorylated amelogenin ... While AMELX regulates mineral transitions over space and time, it is unknown whether and when un-phosphorylated amelogenin ... the shorter leucine-rich amelogenin protein (LRAP), and the exon 4-containing P190 in forming enamel, all within the context of ...
leucine-rich amelogenin peptide: translation product of an alternatively spliced transcript; amino acid sequence has been ... leucine-rich amelogenin peptide (LRAP). Subscribe to New Research on leucine-rich amelogenin peptide ...
Alternative names for Amelogenin Y antibody. AMELY, AMGL, AMGY, Amelogenin Y isoform ... Recombinant protein of human amelogenin, Y-linked (AMELY). Human. > 80 % Preparation: Recombint protein was captured through ... Background of Amelogenin Y antibody. Kit Component:. - KN212029G1, AMELY gRNA vector 1 in pCas-Guide vector. - KN212029G2, ...
... and the Leucine-Rich Amelogenin Peptide (LRAP(+P)), an alternative amelogenin splice product, affects protein assembly and ... and the Leucine-Rich Amelogenin Peptide (LRAP(+P)), an alternative amelogenin splice product, affects protein assembly and ... Based on these findings, we hypothesize that the phosphorylation of full-length amelogenin and LRAP induces conformational ... Based on these findings, we hypothesize that the phosphorylation of full-length amelogenin and LRAP induces conformational ...
Using amelogenin as a case study and a bioinformatics scoring matrix, we identified regions within amelogenin that are shared ... One 22-amino acid long peptide regions referred to as amelogenin-derived peptide 5 (ADP5) was shown to facilitate cell-free ... "Cementomimetics-constructing a Cementum-like Biomineralized Microlayer via Amelogenin-derived Peptides." International Journal ...
Vega: OTTMUSG19595 (Amelx, amelogenin X chromosome)*CCDS: 28475.1, 41213, 41214*OMIM: AMELOGENIN; AMELX, AMELOGENESIS ... amelogenin, X-linked. Synonyms: Amel, Amg, M100888, Rgsc888. Gene nomenclature, locus information, and GO, OMIM, and PMID ...
Background Recombinant amelogenin protein (RAP) is reported to induce complete root apex formation in dog model when used as ... Recombinant amelogenin RAP R-amelogenin Regeneration Apexification Root canal therapy Non-vital teeth Open apex Immature teeth ... A total of 240 root canals were divided into two groups: a recombinant amelogenin protein (180 amino acid mouse amelogenin) ( ... Amelogenin splicing isoforms, leucine-rich amelogenin peptide (LRAP), induces osteogenesis in various cell types by activating ...
The effect of amelogenins (Xelma^,TM,) on hard-to-heal venous leg ulcers * * VOWDEN Peter ...
While it has long been known that amelogenin is essential for the proper development of enamel, its role has generally been ... In this article we report the successful purification of a recombinant mouse amelogenin protein and demonstrate that it has ... Using the TOPGAL transgenic mouse we showed that amelogenin expression in vivo is localized mainly around the root, the ... To determine the regulatory function of canonical Wnt signaling by amelogenin, we used TOPGAL transgenic mice. These mice ...
Dans cet article, on décrit un protocole de fabrication dun hydrogel de chitosane-amélogénine à la reconstruction de lémail...
Presentation] Amelogenin Modulates Macrophage Phenotype During Inflammatory Responses.2016. *. Author(s). Kensuke Yamamichi, ... Presentation] Grp78 is crucial for cell migration induced by amelogenin in a human periodontal ligament cells.2016. *. Author(s ... Presentation] Grp78 Is Critical For Amelogenin-induced Cell Migration In PDLSCs2015. *. Author(s). Takao Fukuda, Kyosuke Toyoda ... Presentation] Molecular basis of amelogenin-induced periodontal tissue regeneration2016. *. Author(s). Takao Fukuda, Terukazu ...
Amelogenin in cranio-facial development: the tooth as a model to study the role of amelogenin during embryogenesis ZORA Wartung ... Download PDF Amelogenin in cranio-facial development: the tooth as a model to study the role of amelogenin during ... Amelogenin in cranio-facial development: the tooth as a model to study the role of amelogenin during embryogenesis. Journal of ... Recently, some amelogenin isoforms were found to bind to the cell surface receptors; LAMP-1, LAMP-2 and CD63, and subsequently ...
Background Amelogenin is an extracellular matrix protein well known for its. Background Amelogenin is an extracellular matrix ... A10, 10?ng/mL; A100, 100?ng/mL; A1000, 1000?ng/mL amelogenin; or without amelogenin dietary supplement (control) (means?? ... ng/mL amelogenin (d). 100?m From the full total outcomes we obtained regarding proliferation, the full-length amelogenin will ... the highest concentration of amelogenin as compared to the unstimulated control. hDPSCs treated with low concentrations present ...
MMP-20 hydrolysis of amelogenin in solution (AMG-S) and bound on HAP (AMG-HAP) were digested and quantified by HPLC. (A) HPLC ... B) Quantified UV areas of 25 kDa amelogenin substrate after digestion. (C) Quantified UV areas of 23 kDa amelogenin derivative ... B) Quantified UV areas of 25 kDa amelogenin substrate after digestion. (C) Quantified UV areas of 23 kDa amelogenin derivative ... The results show that as compared with amelogenin in solution, the HAP-bound amelogenin was hydrolyzed by both MMP20 and KLK4 ...
Amelogenins are a unique group of alternatively spliced proteins. While the full-length amelogenin is known to assemble into ... Synthetic LRAP and recombinant full-length amelogenin (rH175) were used at different concentrations and either immobilized onto ... It was concluded that the ability of amelogenins to self-assemble into nanospheres and to bind to apatite in vitro is not ... nanospheres and alter apatite crystal growth and alignment, the function of the leucine-rich amelogenin peptide (LRAP) in ...
Use Of Full-length Amelogenin For Promoting Nerve Growth Or Regeneration. * Published: Sep 27, 2018 ...
Expression of amelogenin in mouse tooth. Amelx; Algn; Amg; Amgl. Species: mouse Location in mouse genome: chromosome X, ... Mutations in amelogenin gene cause X-linked amelogenesis imperfecta.. Source references: Snead,M.L., Luo,W., Lau,E.C., and, ...
As expected, the secretory function of Satb1−/− SABs was compromised as amelogenins were largely retained in cells. We found ... In line with the deformed apical architecture, amelogenin transport to the apical secretory front and secretion into enamel ... largely amelogenins. EMPs are unidirectionally secreted into the enamel space through their apical cytoplasmic protrusions, or ... space were impeded in Satb1−/− SABs resulting in a massive cytoplasmic accumulation of amelogenins and a thin layer of ...
Human Amelogenin, X-Linked (AMELX) ELISA Kit, Cat#EKU02303. Write a Review Write a Review. × ... The concentration of Amelogenin, X-Linked (AMELX) in the samples is then determined by comparing the O.D. of the samples to the ... Human Amelogenin, X-Linked (AMELX) ELISA Kit, Cat#EKU02303. Rating Required Select Rating. 1 star (worst). 2 stars. 3 stars ( ... The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amelogenin, X-Linked (AMELX). ...
keywords = "Amelogenin, BMP-like activity, Chondrogenic factors, Dentin, Osteogenic factors",. author = "Nebgen, {D. R.} and H ... Identification of the chondrogenic-inducing activity from bovine dentin (bCIA) as a low-molecular-mass amelogenin polypeptide. ... Identification of the chondrogenic-inducing activity from bovine dentin (bCIA) as a low-molecular-mass amelogenin polypeptide. ... These data showed unequivocally that the CIA peptides were derived from bovine amelogenin. The peptides contain the amino- ...
Amelogenin is one of the enamel matrices secreted by ameloblasts. A mutation of the amelogenin gene can cause hereditary dental ... Immunohistochemical study of amelogenin binding proteins in an amelogenin point mutation mouse / Estudio inmunohistoquímico de ... Immunohistochemical study of amelogenin binding proteins in an amelogenin point mutation m ... Full text: Available Index: LILACS (Americas) Main subject: Lysosome-Associated Membrane Glycoproteins / Amelogenin / ...
Amelogenin genes are located on both X and Y sex chromosomes in humans and are a major focus of DNA-based sex estimation ... Amelogenin proteins, AMELX_HUMAN and AMELY_HUMAN, are expressed in the tooth organ and play a major role in mineralization of ... Parker, G.J., et al Sex estimation using sexually dimorphic amelogenin protein fragments in human enamel. Journal of ... Sex estimation using sexually dimorphic amelogenin protein fragments in human enamel. 00 ...
Amelogenin. Amelogenin superfamily. Home. This webpage was best viewed in Mozilla Firefox 3.05 ...
  • [2] The amelogenin gene's location on sex chromosomes has implications for variability both between the X chromosome form ( AMELX ) and the Y chromosome form ( AMELY ), and between alleles of AMELY among different populations. (wikipedia.org)
  • Other sources of amelogenin variation arise from the various isoforms of AMELX obtained from alternative splicing of mRNA transcripts. (wikipedia.org)
  • Differences between the X chromosome and Y chromosome versions of the amelogenin gene (AMELX and AMELY respectively) enable it to be used in sex determination of unknown human samples. (wikipedia.org)
  • In one study in Spain, the amelogenin sex determination test using AMELX (977bps) and AMELY (790bps) bands was performed for 1224 individuals of known gender with a 99.84% (1222/1224) accuracy rate. (wikipedia.org)
  • Amelogenins are a group of protein isoforms produced by alternative splicing or proteolysis from the AMELX gene, on the X chromosome, and also the AMELY gene in males, on the Y chromosome. (wikipedia.org)
  • Amelogenin (AMELX) with its splice variants is the most abundant enamel matrix protein, with only one known phosphorylation site at serine 16 shown in vitro to be critical for regulating mineralization. (frontiersin.org)
  • While AMELX regulates mineral transitions over space and time, it is unknown whether and when un-phosphorylated amelogenin occurs during enamel mineralization. (frontiersin.org)
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Amelogenin, X-Linked (AMELX) were tested 20 times on one plate, respectively. (biomatik.com)
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Amelogenin, X-Linked (AMELX) were tested on 3 different plates, 8 replicates in each plate. (biomatik.com)
  • This assay has high sensitivity and excellent specificity for detection of Amelogenin, X-Linked (AMELX). (biomatik.com)
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amelogenin, X-Linked (AMELX). (biomatik.com)
  • Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Amelogenin, X-Linked (AMELX). (biomatik.com)
  • After TMB substrate solution is added, only those wells that contain Amelogenin, X-Linked (AMELX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. (biomatik.com)
  • The concentration of Amelogenin, X-Linked (AMELX) in the samples is then determined by comparing the O.D. of the samples to the standard curve. (biomatik.com)
  • These results suggest that Amelx* may cause the abnormal distribution of amelogenin binding proteins in the cytoplasm of ameloblasts . (bvsalud.org)
  • Los incisivos de los ratones Amelx* presentaron un color blanco opaco y se observó en microscopio electrónico de barrido que la superficie del diente era áspera. (bvsalud.org)
  • La diferenciación celular secuencial y la forma de los ameloblastos en la etapa de transición en los ratones Amelx* fue irregular en comparación con los ratones silvestres (RS). (bvsalud.org)
  • Además, en ratones Amelx*, el citoplasma de los ameloblastos en etapa de transición fue inmunopositivo para LAMP-1 y LAMP-3. (bvsalud.org)
  • Estos resultados sugieren que Amelx* puede causar distribución anormal de proteínas de unión a amelogenina en el citoplasma de los ameloblastos . (bvsalud.org)
  • Amelogenin proteins, AMELX_HUMAN and AMELY_HUMAN, are expressed in the tooth organ and play a major role in mineralization of enamel, the most taphonomically resistant, archaeologically persistent human tissue. (bioinfor.com)
  • Amplification failure of the amelogenin gene (AMELX) caused by a primer binding site mutation. (univr.it)
  • In particular, protein AMELY (amelogenin isoform Y) is present in the enamel dental tissue of male individuals only, while AMELX (isoform X) can be found in both sexes. (nature.com)
  • One of these proteins is amelogenin, whose gene is translated into two isoforms linked to sexual chromosomes 8 , namely AMELX (present in both sexes) and AMELY (restricted to the male sex only), which constitute 90% of the entire enamel proteome 9 . (nature.com)
  • The aim of this study was to describe the expression of genes, including ameloblastin (Ambn), amelogenin X chromosome (Amelx), and enamelin (Enam) during early (pre-secretory) tooth development. (semanticscholar.org)
  • This method interrogates the sex-typing loci AMELX [amelogenin (amelogenesis imperfecta 1, X-linked)] and AMELY (amelogenin, Y-linked), 2 pentanucleotide-repeat loci (Penta D and Penta E), and the Federal Bureau of Investigation's 13 Combined DNA Index System (CODIS) STR loci. (thefreedictionary.com)
  • This study aims to reveal the spatiotemporal distribution of the cleavage products of the most abundant AMLEX splice variants including the full length P173, the shorter leucine-rich amelogenin protein (LRAP), and the exon 4-containing P190 in forming enamel, all within the context of the changing enamel matrix proteome during mineralization. (frontiersin.org)
  • Previously, we have shown that serine-16 phosphorylation in native full-length porcine amelogenin (P173) and the Leucine-Rich Amelogenin Peptide (LRAP(+P)), an alternative amelogenin splice product, affects protein assembly and mineralization in vitro . (frontiersin.org)
  • Self-assembly and effect on crystal growth of the leucine-rich amelogenin peptide. (escholarship.org)
  • While the full-length amelogenin is known to assemble into nanospheres and alter apatite crystal growth and alignment, the function of the leucine-rich amelogenin peptide (LRAP) in biomineralization is not understood. (escholarship.org)
  • One of the amelogenin splicing isoforms, Leucine-rich Amelogenin Peptide (LRAP) induces osteogenesis in various cell types. (elsevier.com)
  • One 22-amino acid long peptide regions referred to as amelogenin-derived peptide 5 (ADP5) was shown to facilitate cell-free formation of a cementum-like hydroxyapatite mineral layer on demineralized human root dentin that, in turn, supported attachment of periodontal ligament cells in vitro. (ku.edu)
  • This result indicates that MMP20 rapidly cleaves off amelogenin C-terminus, causing the accumulation of the truncated hydrophobic peptide in solution. (nih.gov)
  • This study demonstrates that small peptide domains derived from native protein amelogenin can be utilized to construct a mineral layer on damaged human enamel in vitro. (peereffects.com)
  • Amelogenin Peptide Extract Increases Differentiation and Angiogenic an" by Rene Olivares-Navarrete, Sharon L. Hyzy et al. (vcu.edu)
  • Human MG63 osteoblast-like cells or normal human osteoblasts were treated with recombinant human amelogenin or a 5 kDa tyrosine-rich amelogenin peptide (TRAP) isolated from EMD and the effect on osteogenesis, local factor production, and apoptosis assessed. (vcu.edu)
  • The data suggest that the 5 kDa TRAP-rich sequence is an active amelogenin peptide that regulates osteoblast differentiation and local factor production and prevents osteoblast apoptosis. (vcu.edu)
  • We designed a synthetic amelogenin peptide (SP) derived from EMD, and have previously investigated the biological function of SP. (preprints.org)
  • The amelogenin gene has been most widely studied in humans, where it is a single copy gene, located on the X and Y chromosomes at Xp22.1-Xp22.3 and Yp 11.2 [5]. (wikipedia.org)
  • Is the amelogenin gene reliable for gender identification in forensic casework and prenatal diagnosis? (wikipedia.org)
  • This gene encodes a member of the amelogenin family of extracellular matrix proteins. (nih.gov)
  • To investigate in greater detail the molecular mechanisms involved in the β-catenin signaling pathway, isolated osteoblasts and periodontal ligament cells were exposed to full-length recombinant mouse amelogenin and were evaluated for phenotypic changes and β-catenin signaling using a TOPFLASH construct and the LacZ reporter gene. (ovid.com)
  • A mutation of the amelogenin gene can cause hereditary dental enamel defects known as amelogenesis imperfecta (AI). (bvsalud.org)
  • While the signal of each gene product can vary by more than an order of magnitude, we show close agreement between osteological and amelogenin-based sex estimation and thus demonstrate that the protein-based signal can be obtained reliably from open-air archaeological contexts dating to at least 7300 years ago. (bioinfor.com)
  • Why is The Amelogenin Sex Gene Tested in a Paternity Test? (easydnathailand.com)
  • The amelogenin sex gene, is as suggested by the name, the gene which determines the sex of a person. (easydnathailand.com)
  • The amelogenin sex gene is just one of the sex determining genes and is carried on both the X and Y chromosome. (easydnathailand.com)
  • Labs test the amelogenin sex gene so that they can find out if such an error has happened. (easydnathailand.com)
  • If the amelogenin sex gene is present on both the male and female chromosomes, then how do scientists know that one sample has male DNA and another sample has female DNA? (easydnathailand.com)
  • The amelogenin sex gene is the standard control measure for paternity testing and it makes your test all the more reliable. (easydnathailand.com)
  • In Quantitative Fluorescent Polymerase Chain Reaction (QF-PCR), genetic identification of sex and detection of chromosome X and Y major numerical disorders is routinely performed analyzing PCR products generated from the amplification of the amelogenin gene, in conjunction with other sex-typing markers and short tandem repeat (STR) loci (Cirigliano et al. (univr.it)
  • 2007). In contrast, PCR drop-out of the X-homologue of the amelogenin gene, remaining undetected in females carrying a heterozygous genotype and thus not affecting DNA-based sex tests, have rarely been reported in the literature, and always as a consequence of point mutations in the PCR primers binding sites (Shadrach et al. (univr.it)
  • To determine Sex by analysis of the Amelogenin gene using Polymerase Chain Reaction ( PCR ) method on Deoxyribose nucleic acid ( DNA ) isolated from dental pulp , which was exposed to various environmental conditions created artificially to mimic a forensic scenario. (bvsalud.org)
  • DNA was extracted from dental pulp tissue and sex determination was achieved by amplification of the amelogenin gene through AMEL gene based primers in PCR . (bvsalud.org)
  • Assay of the Amelogenin Y gene (AMELY) on Yp is widely used in DNA-based sex testing, and sometimes reveals males who have interstitial deletions. (edu.au)
  • A simple and efficient non-invasive method was elaborated to detect the genotypic sex from hair and faecal samples using a pair of primers to amplify the X- and Y-linked alleles of the amelogenin gene. (akjournals.com)
  • The amelogenin gene, which is located on human X and Y chromosomes, has been a central system to differentiate males from females in forensic casework and prenatal diagnosis. (ncjrs.gov)
  • In this study, the sex profile of this collection was analysed using molecular sex identification through the amelogenin gene. (scielo.org.za)
  • Although the precise role of amelogenin(s) in regulating the mineralization process is unknown, it is known that amelogenins are abundant during amelogenesis. (wikipedia.org)
  • To better understand the role of amelogenin degradation in enamel biomineralization, the present study was conducted to investigate how the adsorption of amelogenin to hydroxyapatite (HAP) crystals affects its degradation by enamel proteinases, MMP20 and KLK4. (nih.gov)
  • However, the role of amelogenin or amelogenin peptides in mediating osteoblast response to EMD is not clear. (vcu.edu)
  • Full-length amelogenin influences the differentiation of human dental pulp stem cells. (nih.gov)
  • Based on these findings, we hypothesize that the phosphorylation of full-length amelogenin and LRAP induces conformational changes that critically affect its capacity to interact with forming calcium phosphate mineral phases. (frontiersin.org)
  • Conclusions These getting suggest that the part of full-length amelogenin is not restricted to participation in tooth structure. (cylch.org)
  • hDPSCs after 21?times of cultivation using a dietary supplement of 10?ng/mL (b), 100?ng/mL (c) and 1000?ng/mL amelogenin (d). 100?m From the full total outcomes we obtained regarding proliferation, the full-length amelogenin will not appear to significantly have an Decitabine reversible enzyme inhibition effect on the proliferation price of this Decitabine reversible enzyme inhibition teeth pulp cell series ( 0.05) (Fig.?2). (cylch.org)
  • Significant distinctions, 0.05 The exposure of cells to 10?ng/mL individual full-length amelogenin led to hook increase from the growth price (10? (cylch.org)
  • As shown in Figure 3B, the full-length amelogenin in solution was hydrolyzed significantly slower than the protein attached to the crystal surface. (nih.gov)
  • Synthetic LRAP and recombinant full-length amelogenin (rH175) were used at different concentrations and either immobilized onto fluoroapatite substrates (FAP) or immersed into saturated calcium-phosphate solutions. (escholarship.org)
  • These collective findings indicate that phosphorylation induces unique secondary structural changes that may enhance the functional capacity of native phosphorylated amelogenins like LRAP to stabilize an ACP precursor phase during early stages of enamel mineral formation. (frontiersin.org)
  • Peptides specific to the X-chromosome isoform of amelogenin were detected in all samples. (bioinfor.com)
  • Considering the importance of amelogenin protein in dental enamel formation, its ability to control apatite mineralization in vitro , and its potential to be applied in fabrication of future bio-inspired dental material this review focuses on two major subjects: amelogenin and enamel biomimetics. (rsc.org)
  • In these in vitro models, we showed that amelogenin can activate β-catenin signaling. (ovid.com)
  • Preferential and selective degradation and removal of amelogenin adsorbed on hydroxyapatites by MMP20 and KLK4 in vitro. (nih.gov)
  • These results suggest that the adsorption of amelogenin to HAP results in their preferential and selective degradation and removal from HAP by MMP20 and KLK4 in vitro. (nih.gov)
  • It was concluded that the ability of amelogenins to self-assemble into nanospheres and to bind to apatite in vitro is not inevitably an indication for the ability to control apatite crystal growth. (escholarship.org)
  • These observations indicate that amelogenin may be present in cartilage matrix produced in vivo and in vitro and amelogenin may involve cartilage formation through the LAMP-1 signaling pathway. (elsevier.com)
  • In response the authors suggested that while the amelogenin sex test may be accurate in general, other Y chromosome markers such as SRY , STR, or 50f2 can be used for less ambiguous gender identification. (wikipedia.org)
  • [4] Another study in India, however, found 5 of its 270 men studied (1.85%) possessed an AMELY deletion, terming them "deleted-amelogenin males" (DAMs). (wikipedia.org)
  • However, the reliability of the amelogenin test in prenatal diagnosis may sometimes be challenged, mainly because of occasional amplification failure of AMELY in normal males, possibly leading to false gender identification, because of different Yp deletions encompassing the amelogenin locus. (univr.it)
  • AMELY, amelogenin, Y-linked. (thefreedictionary.com)
  • teeth were examined for amelogenin and ameloblastin expression. (umich.edu)
  • immunostaining for amelogenin and ameloblastin was decreased. (umich.edu)
  • Amelogenin-Ameloblastin Spatial Interaction around Maturing Enamel Rods. (abcam.com)
  • Using amelogenin as a case study and a bioinformatics scoring matrix, we identified regions within amelogenin that are shared with a set of hydroxyapatite-binding peptides (HABPs) previously selected by phage display. (ku.edu)
  • These data showed unequivocally that the CIA peptides were derived from bovine amelogenin. (northwestern.edu)
  • The peptides contain the amino-terminal portion of the bovine amelogenin. (northwestern.edu)
  • Amelogenin, EMD's principal component, spontaneously assembles into nanospheres in vivo, forming an ECM complex that releases proteolytically cleaved peptides. (vcu.edu)
  • Both amelogenin and 5 kDa peptides protected MG63s from chelerythrine-induced apoptosis. (vcu.edu)
  • Amelogenins are involved in biomineralization during tooth enamel development. (nih.gov)
  • The amelogenins comprise 90% of the developing extracellular enamel matrix proteins and play a major role in the biomineralization and structural organization of enamel. (uzh.ch)
  • Recombinant amelogenin protein (RAP) is reported to induce complete root apex formation in dog model when used as apexification therapy. (springer.com)
  • The results of the multiplex analysis of the D1S80 and amelogenin loci were comparable to those obtained when each locus was analyzed individually. (unthsc.edu)
  • The preliminary data from the integrin assay show that amelogenin at 0.1 mg/ml also displays a broad up-regulation of several integrins/subunits. (chalmers.se)
  • Amelogenins are type of extracellular matrix protein, which, together with ameloblastins, enamelins and tuftelins, direct the mineralization of enamel to form a highly organized matrix of rods, interrod crystal and proteins. (wikipedia.org)
  • Background Amelogenin is an extracellular matrix protein well known for its part in the organization and mineralization of enamel. (cylch.org)
  • Amelogenin is an extracellular matrix protein that has been reported to promote formation of granulation tissue and repair of chronic venous leg ulcers and elevate the pro-angiogenic vascular endothelial growth factor in dermal fibroblasts.1-3 This study investigated the effect of amelogenin on angiogenesis in an ex vivo sprouting assay and related the findings to the cell surface integrin expression. (chalmers.se)
  • The present results indicate that amelogenin has an important role in many tissues of the cranio-facial complex during mouse embryonic development and differentiation, and might be a multifunctional protein. (uzh.ch)
  • A method has been developed that enables multiplex amplification and simultaneous typing of the loci D1S80 and amelogenin using discontinuous polyacrylamide gel electrophoresis and silver staining. (unthsc.edu)
  • The Investigator 24plex QS Kit is for human identification, allowing multiplex amplification of the CODIS (Combined DNA Index System) core loci, the ESS (European Standard Set) markers, SE33, DYS391, D2S1338, D19S433 and Amelogenin. (qiagen.com)
  • The Investigator 24plex QS Kit uses QIAGEN's fast-cycling PCR technology for the rapid, simultaneous amplification of the CODIS and ESS markers, SE33, DYS391, D2S1338, D19S433 and Amelogenin. (qiagen.com)
  • Integrin assay: Human dermal microvascular endothelial cells (Promocell) were seeded in complete cell growth medium alone or supplemented with 0.1 mg/ml, 1 mg/ml amelogenin or 20 µg/ml fibronectin. (chalmers.se)
  • Conclusions: Amelogenin stimulated micro-vessel outgrowth in the chick aortic arch assay possibly through up-regulation of several integrins and subunits important for cell interaction with the ECM. (chalmers.se)
  • The hardest tooth enamel tissue develops from a soft layer of protein-rich matrix, predominated by amelogenin that is secreted by epithelial ameloblasts in the secretory stage of tooth enamel development. (nih.gov)
  • Amelogenin is one of the enamel matrices secreted by ameloblasts . (bvsalud.org)
  • Since lysosome -associated membrane protein -1 (LAMP-1), -3 (LAMP-3), and 78kDa glucose -related protein (Grp78) were identified as binding proteins of amelogenin , several studies have suggested the involvement of these binding proteins with the cell kinetics of ameloblasts in normal or abnormal conditions. (bvsalud.org)
  • Amelogenin is one of the enamel matrix proteins secreted by ameloblasts during enamel formation in tooth development. (elsevier.com)
  • Bcl9, Bcl9l, and Pygo2 were present in the cytoplasm of ameloblasts, the cells that secrete enamel proteins, and colocalized in these cells with amelogenin, the main component of enamel. (sciencemag.org)
  • Using the TOPGAL transgenic mouse we showed that amelogenin expression in vivo is localized mainly around the root, the periodontal ligament and the alveolar bone. (ovid.com)
  • Even for populations with Amelogenin Null mutations, this means no more failures with the essential gender typing, even with degraded or inhibited samples. (qiagen.com)
  • Amelogenin protein expression was detected in the tooth germ (dental lamina) already at E13.5, much earlier than previously reported (E19). (uzh.ch)
  • Amelogenin is the predominant protein in developing dental enamel. (abcam.com)
  • Amelogenin is the most abundant protein of the enamel organic matrix and is a structural protein indispensable for enamel formation. (elsevier.com)
  • Outcomes Phenotypic appearance Cell morphology and Decitabine reversible enzyme inhibition proliferation The monitoring of morphological adjustments in response to different amelogenin concentrations uncovered no substantial distinctions between your control as well as the activated groupings. (cylch.org)
  • The positive immunoreaction of amelogenin was observed both in proliferation and hypertrophic zone cartilage of growth plate after enzymatic pretreatment in immunostaining. (elsevier.com)
  • A commercially prepared and purified extract of enamel matrix proteins, EMD is composed primarily of amelogenin and has been shown to promote PDL fibroblast proliferation and growth. (wikipedia.org)
  • Amelogenins are a unique group of alternatively spliced proteins. (escholarship.org)
  • Amelogenin genes are located on both X and Y sex chromosomes in humans and are a major focus of DNA-based sex estimation methods. (bioinfor.com)
  • A mistyped amelogenin test has significant consequences in the forensic field. (ncjrs.gov)
  • In this article we report the successful purification of a recombinant mouse amelogenin protein and demonstrate that it has signaling activity in isolated mouse calvarial cells and human periodontal ligament cells. (ovid.com)
  • Amelogenin is the name for a series of closely related proteins involved in amelogenesis , the development of enamel . (wikipedia.org)
  • Bcl9 interacted with amelogenin and proteins involved in exocytosis and vesicular trafficking, suggesting that these proteins function in the trafficking or secretion of enamel proteins. (sciencemag.org)
  • Cloning and Expression Analysis of Human Amelogenin in Nicotiana benthamiana Plants by Means of a Transient Expression System. (nih.gov)
  • Developing human enamel contains about 70% protein, 90% of which are amelogenins. (wikipedia.org)
  • we demonstrate the cellular responses induced by the biological interaction between amelogenin and Grp78 in the human undifferentiated PDL cell line 1-17, which possesses the most typical characteristics of PDLSCs. (nii.ac.jp)
  • Journal Article] Grp78 is Critical for Amelogenin-Induced Cell Migration in a Multipotent Clonal Human Periodontal Ligament Cell Line. (nii.ac.jp)
  • Presentation] Grp78 is crucial for cell migration induced by amelogenin in a human periodontal ligament cells. (nii.ac.jp)