Amelogenin: A major dental enamel-forming protein found in mammals. In humans the protein is encoded by GENES found on both the X CHROMOSOME and the Y CHROMOSOME.Dental Enamel Proteins: The proteins that are part of the dental enamel matrix.Matrix Metalloproteinase 20: A secreted matrix metalloproteinase that is the predominant proteolytic activity in the enamel matrix. The enzyme has a high specificity for dental enamel matrix protein AMELOGENIN.Amelogenesis: The elaboration of dental enamel by ameloblasts, beginning with its participation in the formation of the dentino-enamel junction to the production of the matrix for the enamel prisms and interprismatic substance. (Jablonski, Dictionary of Dentistry, 1992).Dental Enamel: A hard thin translucent layer of calcified substance which envelops and protects the dentin of the crown of the tooth. It is the hardest substance in the body and is almost entirely composed of calcium salts. Under the microscope, it is composed of thin rods (enamel prisms) held together by cementing substance, and surrounded by an enamel sheath. (From Jablonski, Dictionary of Dentistry, 1992, p286)Ameloblasts: Cylindrical epithelial cells in the innermost layer of the ENAMEL ORGAN. Their functions include contribution to the development of the dentinoenamel junction by the deposition of a layer of the matrix, thus producing the foundation for the prisms (the structural units of the DENTAL ENAMEL), and production of the matrix for the enamel prisms and interprismatic substance. (From Jablonski's Dictionary of Dentistry, 1992)Dental Enamel Hypoplasia: An acquired or hereditary condition due to deficiency in the formation of tooth enamel (AMELOGENESIS). It is usually characterized by defective, thin, or malformed DENTAL ENAMEL. Risk factors for enamel hypoplasia include gene mutations, nutritional deficiencies, diseases, and environmental factors.Nanospheres: Spherical particles of nanometer dimensions.Amelogenesis Imperfecta: A clinically and genetically heterogeneous group of hereditary conditions characterized by malformed DENTAL ENAMEL, usually involving DENTAL ENAMEL HYPOPLASIA and/or TOOTH HYPOMINERALIZATION.Tooth Germ: The collective tissues from which an entire tooth is formed, including the DENTAL SAC; ENAMEL ORGAN; and DENTAL PAPILLA. (From Jablonski, Dictionary of Dentistry, 1992)Tooth Calcification: The process whereby calcium salts are deposited in the dental enamel. The process is normal in the development of bones and teeth. (Boucher's Clinical Dental Terminology, 4th ed, p43)Tooth: One of a set of bone-like structures in the mouth used for biting and chewing.Apatites: A group of phosphate minerals that includes ten mineral species and has the general formula X5(YO4)3Z, where X is usually calcium or lead, Y is phosphorus or arsenic, and Z is chlorine, fluorine, or OH-. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Enamel Organ: Epithelial cells surrounding the dental papilla and differentiated into three layers: the inner enamel epithelium, consisting of ameloblasts which eventually form the enamel, and the enamel pulp and external enamel epithelium, both of which atrophy and disappear before and upon eruption of the tooth, respectively.Calcium Phosphates: Calcium salts of phosphoric acid. These compounds are frequently used as calcium supplements.Iguanas: Large herbivorous tropical American lizards.Molar: The most posterior teeth on either side of the jaw, totaling eight in the deciduous dentition (2 on each side, upper and lower), and usually 12 in the permanent dentition (three on each side, upper and lower). They are grinding teeth, having large crowns and broad chewing surfaces. (Jablonski, Dictionary of Dentistry, 1992, p821)Sex Determination Analysis: Validation of the SEX of an individual by inspection of the GONADS and/or by genetic tests.Reptilian Proteins: Proteins obtained from species of REPTILES.Incisor: Any of the eight frontal teeth (four maxillary and four mandibular) having a sharp incisal edge for cutting food and a single root, which occurs in man both as a deciduous and a permanent tooth. (Jablonski, Dictionary of Dentistry, 1992, p820)Durapatite: The mineral component of bones and teeth; it has been used therapeutically as a prosthetic aid and in the prevention and treatment of osteoporosis.Nanotubes: Nanometer-sized tubes composed of various substances including carbon (CARBON NANOTUBES), boron nitride, or nickel vanadate.Odontogenesis: The process of TOOTH formation. It is divided into several stages including: the dental lamina stage, the bud stage, the cap stage, and the bell stage. Odontogenesis includes the production of tooth enamel (AMELOGENESIS), dentin (DENTINOGENESIS), and dental cementum (CEMENTOGENESIS).

Analysis of ancient DNA from a prehistoric Amerindian cemetery. (1/231)

The Norris Farms No. 36 cemetery in central Illinois has been the subject of considerable archaeological and genetic research. Both mitochondrial DNA (mtDNA) and nuclear DNA have been examined in this 700-year-old population. DNA preservation at the site was good, with about 70% of the samples producing mtDNA results and approximately 15% yielding nuclear DNA data. All four of the major Amerindian mtDNA haplogroups were found, in addition to a fifth haplogroup. Sequences of the first hypervariable region of the mtDNA control region revealed a high level of diversity in the Norris Farms population and confirmed that the fifth haplogroup associates with Mongolian sequences and hence is probably authentic. Other than a possible reduction in the number of rare mtDNA lineages in many populations, it does not appear as if European contact significantly altered patterns of Amerindian mtDNA variation, despite the large decrease in population size that occurred. For nuclear DNA analysis, a novel method for DNA-based sex identification that uses nucleotide differences between the X and Y copies of the amelogenin gene was developed and applied successfully in approximately 20 individuals. Despite the well-known problems of poor DNA preservation and the ever-present possibility of contamination with modern DNA, genetic analysis of the Norris Farms No. 36 population demonstrates that ancient DNA can be a fruitful source of new insights into prehistoric populations.  (+info)

Rapid quantification of mixed chimerism using multiplex amplification of short tandem repeat markers and fluorescence detection. (2/231)

Monitoring the engraftment of donor cells after allogeneic blood stem cell transplantation (BSCT) may be important for the early diagnosis of graft failure or relapse of disease. Several techniques have been reported for this purpose. PCR-based assays analyzing polymorphic short tandem repeat (STR) markers are attractive because they are sensitive and can be performed rapidly. The intent of the present study was to test a novel approach for the quantification of mixed chimerism using a commercial multiplex STR assay with fluorescence-based detection for forensic purposes. The feasibility of this assay and the accuracy of quantitative results was tested using serial cell mixtures of unrelated individuals. Sample preparation was optimized to obtain information from minute amounts of starting material, eg from patients with aplasia or from sorted cell populations. Using the STR-PCR, discrimination between donor and recipient was possible in all patients analyzed (n = 25). Cell dilution experiments showed a linear correlation between the cell numbers added and the proportions found, with the limit of detection for a minor cell population being 5%. Comparison of values obtained with standard FISH analysis in patients transplanted from sex-mismatched donors showed an excellent correlation with the STR-PCR results. Taken together, this procedure allows the rapid, versatile and accurate quantification of mixed chimerism, even with minuscule numbers of cells.  (+info)

An immunocytochemical study of amelogenin proteins in the developing tooth enamel of the gar-pike, Lepisosteus oculatus (Holostei, Actinopterygii). (3/231)

Previous studies have demonstrated the morphological similarity of the enamel-like layer found in the teeth of the coelacanth, lungfish and gar-pike to the enamel of tetrapods. In order to clarify the phylogenetic continuity between both structures, tooth germs of the gar-pike were immunocytochemically studied using an anti-bovine amelogenin polyclonal antibody. Intense immunoreaction was shown over the enamel-like matrix layer. Certain cell organelles associated with the secretory pathway of the ameloblasts were recognized as immunoreactive. These results indicate that the enamel-like layer of the gar-pike is a tissue homologous with the mammalian enamel because both possess a common, amelogenin-like substance.  (+info)

Histological analysis and ancient DNA amplification of human bone remains found in caius iulius polybius house in pompeii. (4/231)

Thirteen skeletons found in the Caius Iulius Polybius house, which has been the object of intensive study since its discovery in Pompeii 250 years ago, have provided an opportunity to study either bone diagenesis by histological investigation or ancient DNA by polymerase chain reaction analysis. DNA analysis was done by amplifying both X- and Y-chromosomes amelogenin loci and Y-specific alphoid repeat locus. The von Willebrand factor (vWF) microsatellite locus on chromosome 12 was also analyzed for personal identification in two individuals showing alleles with 10/11 and 12/12 TCTA repeats, respectively. Technical problems were the scarcity of DNA content from osteocytes, DNA molecule fragmentation, microbial contamination which change bone structure, contaminating human DNA which results from mishandling, and frequent presence of Taq DNA polymerase inhibiting molecules like polyphenols and heavy metals. The results suggest that the remains contain endogenous human DNA that can be amplified and analyzed. The amplifiability of DNA corresponds to the bone preservation and dynamics of the burial conditions subsequent to the 79 A.D. eruption.  (+info)

Proteinases in developing dental enamel. (5/231)

For almost three decades, proteinases have been known to reside within developing dental enamel. However, identification and characterization of these proteinases have been slow and difficult, because they are present in very small quantities and they are difficult to purify directly from the mineralizing enamel. Enamel matrix proteins such as amelogenin, ameloblastin, and enamelin are cleaved by proteinases soon after they are secreted, and their cleavage products accumulate in the deeper, more mature enamel layers, while the full-length proteins are observed only at the surface. These results suggest that proteinases are necessary for "activating" enamel proteins so the parent proteins and their cleavage products may perform different functions. A novel matrix metalloproteinase named enamelysin (MMP-20) was recently cloned from tooth tissues and was later shown to localize primarily within the most recently formed enamel. Furthermore, recombinant porcine enamelysin was demonstrated to cleave recombinant porcine amelogenin at virtually all of the sites that have previously been described in vivo. Therefore, enamelysin is at least one enzyme that may be important during early enamel development. As enamel development progresses to the later stages, a profound decrease in the enamel protein content is observed. Proteinases have traditionally been assumed to degrade the organic matrix prior to its removal from the enamel. Recently, a novel serine proteinase named enamel matrix serine proteinase-1 (EMSP1) was cloned from enamel organ epithelia. EMSP1 localizes primarily to the early maturation stage enamel and may, therefore, be involved in the degradation of proteins prior to their removal from the maturing enamel. Other, as yet unidentified, proteinases and proteinase inhibitors are almost certainly present within the forming enamel and await discovery.  (+info)

Identification of CCAAT/enhancer-binding protein alpha as a transactivator of the mouse amelogenin gene. (6/231)

Amelogenin expression is ameloblast-specific and developmentally regulated at the temporal and spatial levels. In a previous transgenic mouse analysis, the expression pattern of the endogenous amelogenin gene was recapitulated by a reporter gene driven by a 2. 2-kilobase mouse amelogenin proximal promoter. To understand the molecular mechanisms underlying the spatiotemporal expression of the amelogenin gene during odontogenesis, the mouse amelogenin promoter was systematically analyzed in mouse ameloblast-like LS8 cells. Deletion analysis identified a minimal promoter (-70/+52) containing a CCAAT/enhancer-binding protein (C/EBP)-binding site upstream of the TATA box. In transient transfection assays, C/EBPalpha up-regulated the promoter activity in a dose-dependent manner. The C/EBP-binding site was necessary for both C/EBPalpha-mediated transactivation and basal promoter activity. Electrophoresis mobility shift assays demonstrated that C/EBPalpha bound to its cognate site in the amelogenin promoter and that the binding was specific. Endogenous C/EBPalpha was detected in LS8 cells, and overexpression of exogenous C/EBPalpha in LS8 cells was able to increase the expression level of the endogenous amelogenin protein. The activity of the amelogenin promoter in rat parotid Pa-4 cells and Madin-Darby canine kidney cells was minimal, ranging from 20 to 30% of the activity in ameloblast-like cells. Transient transfection experiments showed that C/EBPalpha transactivated the mouse amelogenin reporter gene in Pa-4 cells, but not in Madin-Darby canine kidney cells. Taken together, these data indicate that C/EBPalpha is a bona fide transcriptional activator of the mouse amelogenin gene in a cell type-specific manner.  (+info)

Functional antagonism between Msx2 and CCAAT/enhancer-binding protein alpha in regulating the mouse amelogenin gene expression is mediated by protein-protein interaction. (7/231)

Ameloblast-specific amelogenin gene expression is spatiotemporally regulated during tooth development. In a previous study, the CCAAT/enhancer-binding protein alpha (C/EBPalpha) was identified as a transcriptional activator of the mouse amelogenin gene in a cell type-specific manner. Here, Msx2 is shown to repress the promoter activity of amelogenin-promoter reporter constructs independent of its intrinsic DNA binding activity. In transient cotransfection assays, Msx2 and C/EBPalpha antagonize each other in regulating the expression of the mouse amelogenin gene. Electrophoresis mobility shift assays demonstrate that Msx2 interferes with the binding of C/EBPalpha to its cognate site in the mouse amelogenin minimal promoter, although Msx2 itself does not bind to the same promoter fragment. Protein-protein interaction between Msx2 and C/EBPalpha is identified with co-immunoprecipitation analyses. Functional antagonism between Msx2 and C/EBPalpha is also observed on the stably transfected 2.2-kilobase mouse amelogenin promoter in ameloblast-like LS8 cells. Furthermore, the carboxyl-terminal residues 183-267 of Msx2 are required for protein-protein interaction, whereas the amino-terminal residues 2-97 of Msx2 play a less critical role. Among three family members tested (C/EBPalpha, -beta, and -gamma), Msx2 preferentially interacts with C/EBPalpha. Taken together, these data indicate that protein-protein interaction rather than competition for overlapping binding sites results in the functional antagonism between Msx2 and C/EBPalpha in regulating the mouse amelogenin gene expression.  (+info)

Time-related changes of developing enamel crystals after exposure to the tissue fluid in vivo: analysis of a subcutaneously implanted rat incisor. (8/231)

To investigate the effects of tissue fluid on the growth of enamel crystals, upper and lower incisors extracted from 3-week-old Wistar rats were removed of the enamel organ, implanted subcutaneously in the dorsal portion of animals from the same litter, and harvested at 72 h or 1 week after implantation. The grafts were chemically fixed with surrounding tissues and prepared for light and electron microscopy, X-ray microanalysis, or for the immunohistochemistry of amelogenin. Mineralization of implanted enamel layers was examined by contact X-ray microradiography. The immunoreactivities for 25 kD amelogenin in immature enamel decreased sequentially, starting from the surface to the deeper layers; by 1 week after implantation, no positive reactivities remained in the entire enamel layers at the stages of matrix formation and early maturation. In accordance with the loss of enamel proteins, immature enamel gained mineral density until it attained higher radio opacity than that of the adjacent dentin by 1 week. In contrast, the radio opacity of the full thickness of the enamel at early maturation remained low except for a superficial thin layer. Electron microscopy revealed no sign of growth of original enamel crystals, but showed heavy precipitation of electron-dense fine granules of calcium phosphate in all layers of the secretory enamel and the superficial layer of enamel at early maturation, which showed high radio opacity. The Ca/P ratio and electron diffraction patterns of the granular materials precipitated between intrinsic enamel crystals indicated the property of hydroxy apatite or octacalcium phosphate though a characteristic ribbon-like profile of enamel crystals was lacking. These data indicate that the enamel organ blocks exogenous mineral precipitates in growing enamel during the stage of matrix formation and plays an essential regulatory role for fine enamel crystallites to grow into large hexagonal crystals.  (+info)

*Amelogenin

Although the precise role of amelogenin(s) in regulating the mineralization process is unknown, it is known that amelogenins ... Developing human enamel contains about 70% protein, 90% of which are amelogenins. Amelogenins are believed to be involved in ... "deleted-amelogenin males" (DAMs). In response the authors suggested that while the amelogenin sex test may be accurate in ... The amelogenin gene has been most widely studied in humans, where it is a single copy gene, located on the X and Y chromosomes ...

*Index of oral health and dental articles

Amelogenin • American Academy of Cosmetic Dentistry • American Academy of Periodontology • American Association of Endodontists ...

*AMELY

... amelogenin, Y-linked) gene. AMELY is a gene which encodes a form of amelogenin found on the Y chromosome. Amelogenin is a ... "amelogenin"". Genomics. 9 (2): 264-9. doi:10.1016/0888-7543(91)90251-9. PMID 2004775. "Entrez Gene: amelogenin". Human AMELY ... 1992). "The human enamel protein gene amelogenin is expressed from both the X and the Y chromosomes". Am. J. Hum. Genet. 50 (2 ... Amelogenin Y chromosome AMELX Amely (band) GRCh38: Ensembl release 89: ENSG00000099721 - Ensembl, May 2017 GRCm38: Ensembl ...

*AMELX

The protein Amelogenin, X isoform is an isoform of amelogenin that comes from the X chromosome. The protein Amelogenin is a ... Amelogenin, X isoform is a protein that in humans is encoded by the AMELX (amelogenin, X isoform) gene. ... Amelogenin X is a member of the amelogenin family of extracellular matrix proteins. When alternative splicing occurs, it ... Kim JW, Simmer JP, Hu YY, Lin BP, Boyd C, Wright JT, Yamada CJ, Rayes SK, Feigal RJ, Hu JC (May 2004). "Amelogenin p.M1T and p. ...

*Sexing

Markers commonly used include amelogenin, SRY and ZFX/ZFY. Various techniques have been developed using simple polymerase chain ...

*Second Generation Multiplex Plus

SGM Plus also uses the Amelogenin (Amelo) sex-indicating test. SGM Plus differs from SGM in that SGM does not use the markers ...

*Ram Samudrala

Cementomimetics-constructing a cementum-like biomineralized microlayer via amelogenin-derived peptides. Gungormus M, Oren EE, ...

*Enamel matrix derivative

A commercially prepared and purified extract of enamel matrix proteins, EMD is composed primarily of amelogenin and has been ... Biochemistry and molecular biology of amelogenin proteins of developing dental enamel. Arch Oral Bio 1995;40:1-14 Boyan, BD, et ...

*United Kingdom National DNA Database

Amelogenin is used for a rapid test of a donor's sex. However, individuals' skin or blood samples are also kept permanently ...

*Amit Chakrabarti

His other recent work is on self-assembly of amelogenin bio-macromolecules. Amelogenin is involved in the mineral deposition ... 134, 024902 (2011). Kinetics of Nanochain Formation in a Simplified Model of Amelogenin Biomacromolecules, Wei Li et al., ...

*Amelogenesis imperfecta

... tuftelin and amelogenin) as a result of abnormal enamel formation via amelogenesis. People afflicted with amelogenesis ... "A deletion in the amelogenin gene (AMG) causes X-linked amelogenesis imperfecta (AIH1)". Genomics. 10 (4): 971-975. doi:10.1016 ...

*AMBN

Other significant proteins in enamel are amelogenins, enamelins, and tuftelins. This gene encodes the nonamelogenin enamel ...

*Tuftelin

Other significant proteins in enamel are amelogenins, enamelins, and ameloblastins. The human encoding gene for tuftelin (TUFT1 ...

*HDHD1A

"The human enamel protein gene amelogenin is expressed from both the X and the Y chromosomes". Am J Hum Genet. 50 (2): 303-16. ...

*Human tooth

Proteins of note in the development of enamel are ameloblastins, amelogenins, enamelins and tuftelins. It is believed that they ...

*Ganoine

... indeed contains amelogenin-like proteins and has a mineral content similar to that of tetrapod tooth enamel. Zylberberg ... L.; Sire, J. -Y.; Nanci, A. (1997). "Immunodetection of amelogenin-like proteins in the ganoine of experimentally regenerating ...

*Ameloblast

Gallon V, Chen L, Yang X, Moradian-Oldak J. Localization and quantitative co-localization of enamelin with amelogenin. J Struct ... Ameloblasts are cells which secrete the enamel proteins enamelin and amelogenin which will later mineralize to form enamel, the ...

*National Forensic DNA Database of South Africa

The DNA profiles in the NFDD are generated by analysing 15 different STR loci plus Amelogenin for sex determination. Different ... plus Amelogenin for sex determination. There were approximately 80,000 DNA profiles on the DCID in 2007, with the number ...

*Amplicon

"Forensic application of a rapid and quantitative DNA sex test by amplification of the X-Y homologous gene amelogenin". ...

*Amelogenesis

As the prisms in the enamel grow in thickness but not length, proteins (amelogenins and most non-amelogenins) are removed from ... As enamel is secreted, some mineralisation occurs by Ca2+ deposition between nanospheres of amelogenins forming crystallites. ... importantly amelogenins, ameloblastins, enamelins, and tuftelins. The Ca2+ mainly comes from the enamel organ, and not the ...

*Biomimetic material

One is using fragments known to support natural mineralization proteins, such as Amelogenin, Collagen, or Dentin Phosphophoryn ... Proteins of the developing enamel extracellular matrix (such as Amelogenin) control initial mineral deposition (nucleation) and ...

*Second Generation Multiplex

Also contains primers for the Amelogenin sex indicating test. The primers are tagged with the following fluorescent dyes for ...

*BCL9

... interacts with amelogenin and proteins involved in exocytosis and vesicular trafficking, suggesting that these proteins ... and colocalize in these cells with amelogenin, the main component of enamel, encoded by the AMELX gene, which has been already ...

*Aetiocetus

... and amelogenin (AMEL), had not yet taken place in Aetiocetus. For the most part, Aetiocetus retains a primitive tooth count of ...

*ENAM

... amelogenin (AMELX; MIM 300391), ameloblastin (AMBN; MIM 601259), tuftelin (TUFT1; MIM 600087), dentine sialophosphoprotein ( ...
Previously, we have shown that serine-16 phosphorylation in native full-length porcine amelogenin (P173) and the Leucine-Rich Amelogenin Peptide (LRAP(+P)), an alternative amelogenin splice product, affects protein assembly and mineralization in vitro. Notably, P173 and LRAP(+P) stabilize amorphous calcium phosphate (ACP) and inhibit hydroxyapatite (HA) formation, while non-phosphorylated counterparts (rP172, LRAP(−P)) guide the growth of ordered bundles of HA crystals. Based on these findings, we hypothesize that the phosphorylation of full-length amelogenin and LRAP induces conformational changes that critically affect its capacity to interact with forming calcium phosphate mineral phases. To test this hypothesis, we have utilized Fourier transform infrared spectroscopy (FTIR) to determine the secondary structure of LRAP(−P) and LRAP(+P) in the absence/presence of calcium and selected mineral phases relevant to amelogenesis; i.e., hydroxyapatite (HA: an enamel crystal prototype) and (ACP: an
Amelogenin gene expression is spatiotemporally regulated during enamel biomineralization. Studies show that C/EBP alpha is a transactivator of the mouse amelogenin gene acting at the C/EBP alpha cis-element located in the -70/+52 minimal promoter that also contains a reversed CCAAT box (-58/-54). Similar to the C/EBP alpha binding site, this CCAAT box is required for the basal promoter activity. Electrophoretic mobility shift assays demonstrate that NF-Y is directly bound to this reversed CCAAT box. Co-transfection of C/EBP alpha and NF-Y synergistically increases the promoter activity. Protein-protein interactions between C/EBP alpha with NF-Y are identified by a co-immunoprecipitation analysis.; A protein/DNA array technique is utilized to identify a transcriptional factor named YY1. YY1 represses both the basal amelogenin promoter activity and C/EBP alpha-mediated transactivation. Furthermore, YY1 repression is independent of its DNA binding capacity.; C/EBP alpha contains four highly ...
Amelogenin gene expression is spatiotemporally regulated during enamel biomineralization. Studies show that C/EBP alpha is a transactivator of the mouse amelogenin gene acting at the C/EBP alpha cis-element located in the -70/+52 minimal promoter that also contains a reversed CCAAT box (-58/-54). Similar to the C/EBP alpha binding site, this CCAAT box is required for the basal promoter activity. Electrophoretic mobility shift assays demonstrate that NF-Y is directly bound to this reversed CCAAT box. Co-transfection of C/EBP alpha and NF-Y synergistically increases the promoter activity. Protein-protein interactions between C/EBP alpha with NF-Y are identified by a co-immunoprecipitation analysis.; A protein/DNA array technique is utilized to identify a transcriptional factor named YY1. YY1 represses both the basal amelogenin promoter activity and C/EBP alpha-mediated transactivation. Furthermore, YY1 repression is independent of its DNA binding capacity.; C/EBP alpha contains four highly ...
The identification that amelogenin binds to CD63 [11] and LAMP1 [16] led us to hypothesize that amelogenin may be rapidly taken up by the ameloblast cell through receptor-mediated endocytosis [19]. The detailed characterization of the binding regions in amelogenin, CD63 and LAMP1 by the Y2H assay reveals that the region (P155LSPILPELPLEAW168) of amelogenin M180 is responsible for the interaction with CD63 through the domain specified by amino acids 165-205, LAMP1 through the domain specified by amino acids 226-251 and LAMP2 through the domain specified by amino acids 227-259. The binding region (residues 155-168) of amelogenin is hydrophobic, and is predicted to be largely disordered and accessible to the external environment. The region defined by amino acids 165-205 of CD63 is likely to be in a 7 shape within the mushroom-like structure of CD63 EC2.. All of the data presented in Table 2 were derived from the Y2H assays. No significant ambiguity clouded our interpretation, as smaller regions ...
Differences between the X chromosome and Y chromosome versions of the amelogenin gene (AMELX and AMELY respectively) enable it to be used in sex determination of unknown human samples. AMELXs intron 1 contains a 6 bp deletion relative to intron 1 of AMELY. This can be detected at low cost using Polymerase Chain Reaction (PCR) of intron 1, followed by gel electrophoresis. Two bands of DNA, at 106bps and 112bps, are resolved if both the AMELX and AMELY versions of the gene are present (i.e. the sample is from a male) or one band of DNA, at 106bps, if the AMELX version only is present (i.e. the sample is from a female).[3]. However because of AMELY variation among individuals and populations, this method of sex determination is not 100% accurate. Mutation in regions of AMELY intron 1 commonly used as primer annealing sites may disable PCR amplification. A 6bp insertion to AMELY intron 1 results in an amplicon identical in length to that of AMELX. In some males AMELY may be deleted entirely. In any ...
AMELX兔多克隆抗体(ab54507)可与重组片段样本反应并经WB, ELISA实验严格验证,被1篇文献引用。所有产品均提供质保服务,中国75%以上现货。
If you look closely, youll notice that three of the same loci were duplicated. These are internal controls to make sure things are copasetic. There are thirteen loci tested (plus the amelogenin - for sex determination) between the cofiler and profiler plus systems. This is the standard that law enforcement uses ...
For direct STR multiplex amplification of the CODIS and ESS loci, plus SE33, DYS391 and Amelogenin, with an innovative Quality Sensor
For direct STR multiplex amplification of the CODIS and ESS loci, SE33, DYS391 and Amelogenin, with an innovative Quality Sensor ...
Lyrics to Make Me Whole by Amel Larrieux: Darlin I want you to listen, / I stayed up all night, / So I can get this thing right. / And I
leucine-rich amelogenin peptide: translation product of an alternatively spliced transcript; amino acid sequence has been determined
Mice carrying a Y64H amelogenin mutation phenotypically mimic human amelogenesis imperfecta. Affected ameloblasts are characterised by the presence of abnormal cytoplasmic vesicles of retained amelogenin. Protein-protein binding studies using recombinant wild type and Y64H amelogenin revealed that the mutation increased amelogenin-amelogenin binding. This may drive intracellular aggregation of Y64H amelogenin, explaining the abnormal retention. Intracellular protein aggregation causes ER stress which triggers the UPR. The UPR attempts to restore proteostasis but as a last resort triggers apoptosis. SEM of affected enamel showed initially secreted enamel is normal; coincident with UPR in pro-survival mode. The final outer enamel is abnormal; indicative of UPR induced ameloblast apoptosis. Q-RT-PCR was used to measure ER stress related gene expression in affected ameloblasts. Expression levels of ER stress genes increased but not significantly (significance was reached in later studies by others ...
Masica, David L., and Rachel Karchin. "Collections of simultaneously altered genes as biomarkers of cancer cell drug response." Cancer research 73, no. 6 (2013): 1699-1708.. Masica, David L., Patrick R. Sosnay, Garry R. Cutting, and Rachel Karchin. "Phenotype-optimized sequence ensembles substantially improve prediction of disease-causing mutation in cystic fibrosis." Human Mutation (2012).. Masica, David L., and Rachel Karchin. "Correlation of somatic mutation and expression identifies genes important in human glioblastoma progression and survival." Cancer research 71, no. 13 (2011): 4550-4561.. Masica, David L., Jeffrey J. Gray, and Wendy J. Shaw. "Partial high-resolution structure of phosphorylated and non-phosphorylated leucine-rich amelogenin protein adsorbed to hydroxyapatite." The Journal of Physical Chemistry C 115, no. 28 (2011): 13775-13785.. Masica, David L., Sarah B. Schrier, Elizabeth A. Specht, and Jeffrey J. Gray. "De Novo Design of Peptide-Calcite Biomineralization Systems." ...
Principal Investigator:Toyoda Kyosuke, Project Period (FY):2015-08-28 - 2017-03-31, Research Category:Grant-in-Aid for Research Activity start-up, Research Field:Periodontology
The bone matrix and tooth enamel are the hardest substances in the body, and both are formed through the mineralization of proteins secreted by osteoblasts and ameloblasts, respectively. One of the primary components of the bone matrix is collagen, which provides strength, as well as acts as a substrate for mineralization. Amelogenin is the main extracellular matrix protein secreted by ameloblasts and is important for both the inclusion of minerals and the proper structure of tooth enamel. Defects in the secretion or structure of these extracellular matrices compromise the strength and durability of the tissues. Two studies reveal distinct mechanisms of secretory dysregulation that affect proper mineralization of these tissues. Zhao et al. found that the trimeric intracellular cation channel Tric-b, which localizes to the endoplasmic reticulum (ER), was important to secretion of collagen by osteoblasts in mice. Mutations in TMEM38b, the human homolog of Tric-b, are associated with osteogenesis ...
AMBN - AMBN (untagged)-Human ameloblastin (enamel matrix protein) (AMBN) available for purchase from OriGene - Your Gene Company.
Sodium Risedronate Amel is a medicine available in a number of countries worldwide. A list of US medications equivalent to Sodium Risedronate Amel is available on the Drugs.com website.
Apatite crystals and specimens hand selected and individually pictured to allow you to select the right piece for your metaphysical needs.
Article Multi-component supramolecular assembly structures studied by scanning tunnelling microscopy. In this review, recent progress on the multi-component self-assembled structure and assembling mechanism are discussed. The effects of various inter...
Page contains details about porphyrin supramolecular assemblies . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
Az RSS a Really Simple Syndication egy olyan form tum amely lehet v teszi, hogy webhely nk aktualit sait megosszuk nnel s m s weboldalak szerkeszt ivel. A form tumnak t bb verzi ja l tezik, mi a 0.91-es v ltozatot t mogatjuk, csatorn nk ISO 8859-2 karakterk dol st haszn l. ...
A radioaktív bomlás során az instabil atommag szerkezete változik meg A radioaktív bomlás során az instabil atommag szerkezete változik meg. BOMLÁS = új atommag keletkezik, amely részecskesugárzás kibocsátása révén stabilizálódik, tehát a radioaktivitás és a sugárzás összetartoznak. Radioaktív nuklidok keletkezése: magreakciók által, melyekben „célmagok
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Az aldoszteron a mell kvese-k regben termel d szteroid hormon, amely a szervezet s - s v zh ztart s rt felel s. Termel d s vel fokoz dik a szerve
The flexible structure of polymers has enabled them to be useful in a wide variety of medical applications due to the possibility to tailor their properties to suit desired applications. For a long ti
Specimen A1008 produced odd results that suggested a possible female: an X chromosome in the first system and a mix of polymorphic regions corresponding to both the X and Y chromosomes in the second system (Figure 3). Even though the amplification of the Y chromosome was lower, the presence of the Y chromosome indicated this individual was a male and was diagnosed as such. DISCUSSION AND CONCLUSION. Using both systems of molecular sex identification, 13 (41.93%) specimens in the sample produced results, all of which indicated that the specimens were male (Table 1). Based on this data, seven (53.84%) specimens in the sample produced the same result in both systems and two specimens produced different results in each system. Results were generated for four specimens using only one method: one using the first system and three using the second system of limited molecular preservation of the DNA molecules in the area of interest on the amelogenin gene. Both systems produced results for the same ...
In the framework of the project "Immigration and mobility in mediaeval and post-mediaeval Norway" molecular genetic analyses were performed on 97 pre-modern human remains including genetic sexing and Y-chromosomal DNA typing. All samples were subjected to molecular genetic analyses of the sex using "Genderplex" consisting of two diff erent regions of the amelogenin gene, SRY and four X-STR loci. From 90% of the extracted remains (n=87) sex assignment was possible. Of these, 49 (56.3%) brought a genetically male result. All of these DNA extracts were subjected to Y-STR analysis using Yfiler Plus PCR Amplification Kit (Thermo Fisher Scientifi c) and/or PowerPlex Y23 System (Promega). At least partial Y-STR profiles were obtained from all samples. A detailed comparison between mediaeval/post-mediaeval and contemporary Y-chromosomes was performed by searching the obtained haplotypes (HTs) in the Y Chromosome Haplotype Reference Database (YHRD: https://yhrd.org) comprising 154,329 haplotypes from 991 ...
A supramolecular assembly or "supermolecule" is a well defined complex of molecules held together by noncovalent bonds. While a supramolecular assembly can be simply composed of two molecules (e.g., a DNA double helix or an inclusion compound), it is more often used to denote larger complexes of molecules that form sphere-, rod-, or sheet-like species. Micelles, liposomes and biological membranes are examples of supramolecular assemblies. The dimensions of supramolecular assemblies can range from nanometers to micrometers. Thus they allow access to nanoscale objects using a bottom-up approach in far fewer steps than a single molecule of similar dimensions. The process by which a supramolecular assembly forms is called molecular self-assembly. Some try to distinguish self-assembly as the process by which individual molecules form the defined aggregate. Self-organization, then, is the process by which those aggregates create higher-order structures. This can become useful when talking about liquid ...
Fluvoxamine Maleate Amel is a medicine available in a number of countries worldwide. A list of US medications equivalent to Fluvoxamine Maleate Amel is available on the Drugs.com website.
A Pécsi Riport tartalmaz beszámolókat a város kulturális, közéleti, sport és minden olyan történéséről, amely Pécs város polgárait érdekelheti. Napilapunk megbízható forrásokból szerzi információit, melyet egy-egy esemény után szinte azonnal olvashat, képekkel és videóval.
A Pécsi Riport tartalmaz beszámolókat a város kulturális, közéleti, sport és minden olyan történéséről, amely Pécs város polgárait érdekelheti. Napilapunk megbízható forrásokból szerzi információit, melyet egy-egy esemény után szinte azonnal olvashat, képekkel és videóval.
A Szentágothai János Kutatóközpont a PTE korszerű, nemzetközi tudományszervezési és menedzsment normák szerint kialakított új intézménye, amely az élettudományi, élettelen természettudományi, valamint környezettudományi oktatás.... Tovább. ...
Ny jtott felsz v d s filmtabletta, gy folyamatosan l tja el a szervezetet tablett nk nt 1200 mg C-vitaminnal, amely hozz j rul az immunrendszer s az idegrendszer norm l m k d s hez. Antioxid nsk nt t mogatja a sejtek oxidat v stresszel szembeni v delm t, a f radts g s kif rad s cs kkent s t, fokozza a vas felsz v d s t. T mogatja a kollag nk pz d st, a csontozat, a porcok, a b r, az erek, a fogak s a fog ny norm l llapot nak fenntart s t ...
Page contains details about ITO/VB-FNPD/mixed nanospheres/TPBi/LiF/Al . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
Learn more about In His Own Words: Living With Coronary Artery Disease at Reston Hospital Center Bill, 72, never suspected he had a heart problem, until he suffered...
Tooth enamel formation. Coloured scanning electron micrograph (SEM) of a freeze-fractured section through a tooth, showing the enamel-forming cell layer (green). This epithelium comprises a single layer of columnar cells called ameloblasts. The fracture plane passes up into the tooth from the enamel surface (orange, bottom left). The ameloblast layer has detached from the enamel in which it is normally embedded. Enamel is a hard ceramic layer that covers and protects the teeth. The other end of the ameloblasts originate in the internal tooth tissue (brown, across top). Magnification unknown. - Stock Image P486/0080
Accurate identification of the biological sex of ancient remains is vital for critically testing hypotheses about social structure in prehistoric societies. However, morphological methods are imprecise for juvenile individuals and fragmentary remains, and molecular methods that rely on particular sex-specific marker loci such as the amelogenin gene suffer from allelic dropout and sensitivity to modern contamination. Analyzing shotgun sequencing data from 14 present-day humans of known biological sex and 16 ancient individuals from a time span of 100 to ~70,000 years ago, we show that even relatively sparse shotgun sequencing (about 100,000 human sequences) can be used to reliably identify chromosomal sex simply by considering the ratio of sequences aligning to the X and Y chromosomes, and highlight two examples where the genetic assignments indicate morphological misassignment. Furthermore, we show that accurate sex identification of highly degraded remains can be performed in the presence of ...
The deposition of enamel is marked by the formation of growth lines, which reflect incremental growth. Although periodic markings have been observed in enamel of non-mammalian vertebrates, the cross-striation interval and the pattern of enamel deposition have not been formally investigated. Here a structural study was made of the enamel in four non-mammalian vertebrates, with emphasis on periodic markings. Teeth from Rana catesbeiana, Tropidurus torquatus, Caiman crocodilus and a Canadian carnosaur were analysed. Enamel of T. torquatus and R. castebeiana was aprismatic; that of C. crocodilus and the carnosaur was formed by large, prism-like structures. Conspicuous incremental lines were observed in the enamel of the three living species, which presented a cross-striation repeat smaller than the prism cross-striations of mammalian enamel. Incremental lines of carnosaur enamel had a mean repeat interval similar to that of mammalian prism cross-striations. As metabolic activity in ectotherms is ...
Bioactive glass (BG) can directly bond to living bone without fibrous tissue encapsulation. Key mechanistic steps of BGs activity are attributed to calcium phosphate formation, surface hydroxylation and fibronectin (FN) adsorption. In the present study, self-assembled monolayers (SAMs) of alkanesilanes with different surface chemistry (OH, NH2 and COOH) were used as a model system to mimic BGs surface activity. Calcium phosphate (Ca-P) was formed on SAMs by immersion in a solution that simulates the electrolyte content of physiological fluids. FN adsorption kinetics and monolayer coverage was determined on SAMs with or without Ca-P coating. The surface roughness was also examined on these substrates before and after FN adsorption. The effects of FN-adsorbed, Ca-P-coated SAMs on the function of MC3T3-E1 were evaluated by cell growth, expression of alkaline phosphatase activity and actin cytoskeleton formation. We demonstrate that, although the FN monolayer coverage and the root mean square (rms)
Tooth enamel is the hardest substance of our body, composed entirely of the mineral apatite deposited on a substrate of three unique enamel matrix proteins.
Promega announces the launch of GenePrint®10 System for human cell line authentication and sample identification. GenePrint 10 includes the eight loci recommended by the ANSI Standard (ASN-0002) Authentication of Human Cell Lines: Standardization of STR Profiling, 1 plus Amelogenin for gender identification and the highly polymorphic D21S11 locus for additional power of discrimination. Short Tandem Repeat (STR) analysis begins with amplification, and the GenePrint 10 System contains all<
The ForenSeq™ DNA Signature Prep Kit (ForenSeq Kit) is designed to detect more than 200 forensically relevant markers in a single reaction on the MiSeq FGx™ Forensic Genomics System (MiSeq FGx System), including Amelogenin, 27 autosomal short tandem repeats (A-STRs), 7 X chromosomal STRs (X-STRs), 24 Y chromosomal STRs (Y-STRs) and 94 identity-informative single nucleotide polymorphisms (iSNPs) with the option to contain 22 phenotypic-informative SNPs (pSNPs) and 56 ancestry-informative SNPs (aSNPs). In this study, we evaluated the MiSeq FGx System on three major parts: methodological optimization (DNA extraction, sample quantification, library normalization, diluted libraries concentration, and sample-to-cell arrangement), massively parallel sequencing (MPS) performance (depth of coverage, sequence coverage ratio, and allele coverage ratio), and ForenSeq Kit characteristics (repeatability and concordance, sensitivity, mixture, stability and case-type samples ...
NEW YORK (GenomeWeb News) - Nanosphere today said that its second-quarter revenues jumped 160 percent year over year, but the firm fell short of Wall Streets estimate for the top line.
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Have you ever wondered about tooth enamel? What is it? How important is it? How can you protect it? Here are the answers to all… Read more at Colgate.com
A NATO-kódnév első betűje utal az eszköz jellegére, rendeltetésére. A vadászrepülőgépeknél az első betű F (az angol fighter szó első betűjéből), a bombázóknál B (=bomber), a szállító repülőgépeknél C (=cargo), a helikoptereknél H, a föld-föld rakétáknál S, a légvédelmi rakétáknál G, az előző kategóriákba be nem sorolható repülőgépek esetében M. A merevszárnyú gépeknél a légcsavaros repülőgépek esetében a NATO-kódnév egyszótagú, sugárhajtású gépeknél kétszótagú. Például a sugárhajtású MiG-29 vadászrepülőgép NATO-kódneve Fulcrum, amely F-fel kezdődik és kétszótagú. A Tu-95 légcsavaros gázturbinás bombázó repülőgép NATO-kódja Bear, amely B-vel kezdődik és egyszótagú. Ugyanazon típus különféle alváltozatainál a NATO-kódnevet az ABC egy-egy betűjével egészítették ki. Például a Tu-95MSZ változat NATO-kódja Bear-H.. Néhány haditechnikai eszközre az Egyesült Államok Védelmi ...
An underlying theme of this meeting is the search for potential biophysical relationships between the extracellular matrix and the cytoskeleton. We found that the fruit fly, Drosophila melanoqaster....
cdna chromosome:GRCm38:5:88456011:88468531:1 gene:ENSMUSG00000029288 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Ambn description:ameloblastin [Source:MGI Symbol;Acc:MGI:104655 ...
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A Ne zavarj mód egy olyan beállítás, amely minden hangot és rezgést elnémít, és különösen olyankor hasznos, ha színházban vagy olyan környezetben viseli az órát, ahol a megszokott, de néma üzemelés a kívánatos.. A Ne zavarj mód megakadályozza azt is, hogy az óra egy inaktivitási időszak elteltével hibernálás módba kapcsoljon. Ezt a beállítást a napi pulzusértékkel együtt engedélyezheti (lásd Napi pulzusmérés), hogy napi 24 órában, a hét minden napján nyomon követhesse pulzusát.. A Ne zavarj mód engedélyezéséhez: ...
A hat konyabb ter pia s a hosszabb t l l s rdek ben a daganatos beteg kezel se sor n az egy nre szabott, a szervezet t nyleges sz ks glet nek megfelel t pl lts gi llapot fenntart s ra kell t rekedni. Amennyiben a beteg ember m r nem k pes naponta hagyom nyos m don elegend mennyis g telt mag hoz venni, t pl lts gi llapot nak (tov bbi) roml s t megel zve speci lis eszk z k s gy gyt pszerek alkalmaz s n alapul n. mesters ges t pl l st kell v gezni.. Sokan s sokszor pr b lt k a mesters ges t pl l s fogalm t betegk zelibb s rthet bb tenni. A mesters ges jelz miatt idegenked st s bizalmatlans got kiv lt kifejez s a mindennapi orvosi sz haszn latban gyakran el fordul.. Szervezet nk m k d s hez t panyagokra s az ebb l sz rmaz energi ra van sz ks g nk. Energi t ig nyel az is, ha csup n feksz nk, l legz nk, dobog a sz v nk, fenntartjuk testh m rs klet nket, gondolkodunk, em szt nk, m k dik az immunrendszer nk. Az ehhez sz ks ges energi t nevezz k alapanyagcser nek, amely a teljes testi s lelki ...
Korszer , kett s metaliz lt f lia csomagol sban a hat anyag stabilit s nak v delme rdek ben. Speci lis technol gi val el ll tott, ny jtott felsz v d s filmtabletta, gy folyamatosan l tja el a szervezetet a C-vitaminnal. Magas hat anyag tartalm , tablett nk nt 1500 mg C-vitaminnal, amely hozz j rul az immunrendszer s az idegrendszer norm l m k d s hez, a megfelel pszichol giai funkci fenntart s hoz. Antioxid nsk nt t mogatja a sejtek oxidat v stresszel szembeni v delm t, a f radts g s kif rad s cs kkent s t, fokozza a vas felsz v d s t. T mogatja a kollag nk pz d st, a csontozat, a porcok, a b r, az erek, a fogak s a fog ny norm l llapot nak fenntart s t ...
Magas fedok pess gu, foly kony korrektor, amely t k letesen fedi a kapill ris erek s r l s bol eredo s t t karik kat, duzzanatokat a szem k r l s amellett a speci lis sszetevoknek,k sz nhetoen kezeli is azokat ak r 16 r n t. ,Klinikailag bizony tott,
A Project Torque (level-r) izgalmas online aut verseny-szimul tor. Amely a l tv nyos grafikai megjelen t sek s lethû hangeffektusok mellett egyar nt alkalmas az on- s offroad versenyekre: sz mtalan v rosban s extr m terepen. T bbf le j t km d, 8-32 j t kos futamok, chat, tuning lehetõs g, bajnoks g jellem...
A dukt lis papill ma j indulat h m eredet daganat, amely hajlamos rosszindulat elfajul sra. Legjellemz bb t nete a v rz eml bimb . Semmik pp ne hanyagolja el, jelentkezz k m t tre s adjon h l t a...
A helyzetkijelzés az a mód, ahogyan a GPS-vevő helyzete megjelenik az órán. Minden kijelzési mód ugyanarra a helyre hivatkozik, csak a megjelenítésben térnek el.. A kijelzési módot módosíthatja az óra beállításai között a Navigation (Navigálás) » Position format (Helyzetformátum) menüpontban.. A leggyakrabban használt fokhálózat a szélesség/hosszúság, amely háromféle formátumban megjeleníthető:. ...
Mutations in human and/or mouse homologs are associated with this disease. Synonyms: AIH1; amelogenesis imperfecta hypomaturationtype with snow-capped teeth; amelogenesis imperfecta type IE; X-linked amelogenesis imperfecta 1; X-linked amelogenesis imperfecta hypoplastic/hypomaturation 1; X-linked enamel hypoplasia
Mutations in the AMELX, ENAM, MMP20, KLK-4, FAM83H, WDR72, C4orf26, SLC24A4 LAMB3 and ITGB6 genes have been found to cause amelogenesis imperfecta (non-syndromic form). AMELX and ENAM encode extracellular matrix proteins of the developing tooth enamel and KLK-4 and MMP20 encode proteases that help degrade organic matter from the enamel matrix during the maturation stage of amelogenesis. SLC24A4 encodes a calcium transporter that mediates calcium transport to developing enamel during tooth development. Less is known about the function of other genes implicated in amelogenesis imperfecta.. Researchers expect that mutations in further genes are likely to be identified as causes of amelogenesis imperfecta.. Types include:. Amelogenesis imperfecta can have different inheritance patterns depending on the gene that is altered. Mutations in the ENAM gene are the most frequent known cause and are most commonly inherited in an autosomal dominant pattern. This type of inheritance means one copy of the ...
Looking for online definition of Enamelysin in the Medical Dictionary? Enamelysin explanation free. What is Enamelysin? Meaning of Enamelysin medical term. What does Enamelysin mean?
Stable carbon isotope analysis is now an established tool for investigating the diets of fossil taxa, but carbon isotopes provide us with limited information about an animals ecology. Recent research suggests that mammalian oxygen isotope compositions might also prove profitable sources of ecological information. If we are to exploit this resource, however, we must improve our nascent understanding of oxygen isotope compositions within modern foodwebs. To this end, we have analyzed the oxygen and carbon isotope compositions of nine ecologically diverse, sympatric taxa from Morea Estate, Mpumalanga Province, South Africa. These data show that the Morea Estate faunivores are depleted in 18O compared to herbivores, and among the herbivores, frequent drinkers are relatively depleted in 18O. While more research is needed to address the mechanisms for and universality of these patterns, these results show oxygen isotope analysis to be a promising avenue of paleoecological research. ...
Detail of the period when the root sheath cells (blue) secrete enamel matrix proteins. Following formation of the protein matrix on the surface of the mineralizing dentin, cementoblasts (Red) start producing cementum (Light blue) which anchors collagen fibers.. The Next Generation. In the mid 1980s, a revolutionary breakthrough in the basic biology of tooth development took place at the Karolinska Institutes department of Oral Pathology in Stockholm. The discovery revealed a native complex of enamel matrix proteins and the key if not crucial , role they play in the development of tooth-supporting tissues. These enamel related proteins mediate the formation of acellular cementum on the root of the developing tooth, providing a foundation for all of the necessary tissues associated with a functional periodontal attachment.. This groundbreaking discovery has fueled the development of a new generation in periodontal therapy, and makes the recreation of lost tooth support an attainable goal in the ...
The pseudoautosomal (PA) region of the mammalian genome is the region of the X and Y chromosomes that shares extensive DNA sequence homology and is of special interest because it may play an essential role during male meiosis. We have identified three telomere-related restriction fragments from the PA region of the mouse genome, using an oligonucleotide probe composed of the mammalian telomere consensus sequence TTAGGG. PA assignment of two C57BL/6J-derived fragments was initially suggested by analysis of DNAs from progeny sired by C57BL/6J males carrying the rearranged Y chromosome, Y*: the hybridization intensity of both fragments was concordant with the sex-chromosome complement of the offspring. Further analysis indicated that both fragments were present in female and male F1, mice regardless of the sex of their C57BL/6J parent--a criterion for autosomal or PA linkage. Both fragments were closely linked to each other and located on the X chromosome distal to amelogenin (Amg)--in
KODAK X-SIGHT Western Kits are built on X-SIGHT Nanospheres, which are available in four distinct wavelengths including three in the near-IR range. The kits are offered in several different wavelength combinations that include up to three X-SIGHT Nanosphere Conjugates, making them ideal for multiplex applications. Each kit is optimized for 10 blots and includes best-in-class membranes and buffers to achieve high signal-to-noise ratios. A separate Accessory Kit is also available for use with any of the X-SIGHT Nanosphere Conjugates or any other commercially available fluorescently-labeled secondary antibody conjugate (nanoparticle or dye ...
It is believed by some that when a butterfly lands on you it brings good luck. Add a little symbolic good luck to your growing collection of animal jewelry with the purchase of our newest silvertone butterfly brooch
How to Strengthen Tooth Enamel. Weak tooth enamel, which can be caused by diet, dry mouth, gastrointestinal issues, and stress, leaves you susceptible to tooth problems like cavities, chipping, and sensitivity. Demineralization, or...
Normally developed enamel will have a smooth, white appearance. However, when conditions in the environment interfere with the development of tooth enamel, teeth can take on a discolored, pitted or otherwise unusual appearance.
Different foods and drinks can affect your teeth. Foods and drinks that contain high amounts of sugars and carbohydrates help create bacteria in your mouth that hurt your tooth enamel soon after you enjoy a snack or meal. That is why it is very important that you brush your teeth after you eat, so that Read more » ...
Abnormal environmental or physical conditions can interfere with the development of tooth enamel, causing it to take on a discolored, pitted or otherwise unusual appearance.
A Magyarorsz gon ad illet s ggel rendelkez mag nszem lyekt l levont kamatad m rt ke 15%. A Magyarorsz gon ad illet s ggel nem rendelkez mag nszem lyek mentes lhetnek a magyar kamatad al l, vagy a magyarn l kedvez bb kulccsal ad zhatnak nemzetk zi ad egyezm ny alapj n. Az a k lf ldi mag nszem ly mentes lhet a magyar kamatad al l, vagy ad zhat a magyarn l kedvez bb kulccsal, aki olyan llamban rendelkezik illet s ggel, amely llam Magyarorsz ggal ad egyezm nyt k t tt, s az egyezm ny alapj n lehet s g van a magyar kamatad helyett az egyezm ny szerinti kedvez bb ad kulcs alkalmaz s ra. Az illet s g igazol sa t rt nhet az n. CRS nyilatkozattal vagy illet s gigazol s bemutat s val. A CRS a p nz gyi sz ml kkal kapcsolatos inform ci k automatikus cser j r l sz l nemzetk zi meg llapod s, amely alapj n a Banknak meg kell llap tania gyfelei ad illet s g t. Ezen illet s g vizsg lata sor n kell nnek nyilatkoznia ad illet s g r l ( n. CRS nyilatkozat). A CRS nyilatkozatot b rmely bankfi kunkban megteheti. CRS ...
Waking - Ralph awoke and puled his baseball cap off the nightstand, but he couldnt help but notice the long pink nails on the ends of his fingers. They made it dif... ...
Building your knitting needle collection? Do it stylishly and affordably with Susan Bates Crystallite Knitting Needles! Feather-light for comfort, each colorful package comes with a set of four useful sizes, one for worsted weight yarns: US 8 (5 mm), US 9 (5.5 mm), US 10 (6.0 mm), and US 10.5 (6.5 mm); and one for chunky weight yarns: US 11 (8 mm), US 9 (5.5 mm), and US 15 (10.0 mm).
Enamel, the first layer of the tooth that is visible in the mouth, is a porous, calcified substance. Learn about the important roles it plays.
Scientists have uncovered evidence that the enamel that protects our teeth originated in the scales of fish that lived 400 million years ago.
Mivel az eml s llatok sok id t s energi t fektetnek ut daik felnevel s be, kialakult egy rzelmi reakci k szlet, amely arra serkenti a sz l t, hogy siessen bajbajutott vagy szenved ut d nak megseg t s re. J l ismert, milyen ellen llhatatlan h v jel az llat vagy ember kicsiny nek s r sa. A fejlett eml s kn l, s k l n sen az embern l ez az ut dra ir nyul seg t si-gondoskod si k sztet s kiterjedt a csoport minden tagj ra, gy m sok szenved se nk ntelen r szv tet s seg teni akar st v lt ki. A szenved ssel s kegyetlens ggel szembeni ellen rz sekb l alakult ki a szenved st, f jdalmat elh r t szem lyek nagyra rt kel se. Minden t rsadalomban tisztelik az nfel ldoz st, vagy a gy gy t szem lyeket. Ezeket az em ci kat az rtalommal s gondoskod ssal kapcsolatos mor lis elvek alapj nak tekintj k. A szoci lis letet l eml s kn l s az embern l a csoporttagok k zti sz vets gk t s s kooper ci nagy t l l si el nyt biztos tott, ez rt kialakultak azok az rzelmek, amelyek a k lcs n s nzetlens get s egy ttm k d si k szs ...
Biol gia fogalomt r. 5-3 ir ny: a DNS sz lainak 5 v ge a nukleotidot fel p t rib z t dik sz natomj ra, 3 v ge a l nc v g n l v nukleotida rib z nak harmadik sz natomj ra utal. Az j sz l keletkez se mindig az 5 v gt l halad a 3 v g fel .. A, Acetil-csoport: a pirosz l savb l vezethet le sz n-dioxid kihasad ssal.. Acetilez s: a KoA-molekul k megk tik az acetil-csoportokat. Helye a mitokondrium k ls membr nja.. Adekv t inger: az az inger, amely az adott rz kszervet a legk nnyebben ingerli, amellyel szemben a legkisebb az rz kszerv ingerk sz be.. Adenin: k tgy r s, nitrog ntartalm szerves b zis.. ADP: adenozin-difoszf t, mely foszf tcsoport bek t s vel ATP-v tud talakulni. Ez az talakul s energiatermel folyamatok eset ben val sul meg.. Aerob k r lm ny: oxig nben gazdag llapot.. Agyvel : a k zponti idegrendszer agykopony ban l v r sze.. Akci s potenci l: a sejt inger letbe ker l s nek eredm nye. A beindul ioneloszl sbeli v ltoz s a membr n k t oldala k z tt potenci lv ltoz st alak t ki.. Aktin: ...
Lan GJ,Zhang XM,Zhang XM,et al. Yolk-shell nanospheres with soluble aminopolystyrene as a reservoir for Pd NPs[J]. RSC Advances,2016,5(0):35730 ...
Amelogenesis imperfecta is a genetic disorder that causes defective enamel development in both the primary and permanent dentitions. Significant tooth structure damage often results in various pulpal symptoms, occlusal disharmony, impaired function, and esthetic disfigurement. These problems pose great challenges to the clinician when rehabilitating patients with amelogenesis imperfecta. This case report describes an uncomplicated and logical way to reorganize, temporize, and completely restore an extensively damaged dentition caused by amelogenesis imperfecta ...
Looking for information on Amelogenesis imperfecta? Medigest has all you need to know about Amelogenesis imperfecta - Symptoms and Signs, Causes, Treatments and definition
Amelogenesis imperfecta is an inherited defect of dental enamel formation that shows both clinical and genetic heterogeneity. In the hypoplastic type of AI, the enamel is of normal hardness but does not develop to normal thickness. The thinness of the enamel makes the teeth appear small. Radiographically, enamel contrasts normally from dentin. The surface of the enamel can vary, showing smooth, rough, pitted, or local forms (Witkop, 1988).
In a group of families in northern Sweden, a mutation in the ENAM gene (predicted to produce a highly truncated protein) results in the local hypoplastic form of autosomal dominant amelogenesis imperfecta. In this study, sections of deciduous teeth from members of 3 of these families .... ...
Insulin-dependent type-1 diabetes mellitus (DM) and oral diseases are closely interrelated. Poor metabolic control in diabetics is associated with a high risk of gingivitis, periodontitis and tooth loss. Salivary flow declines in diabetics and patients suffer from xerostomia. Reduced saliva predisposes to enamel hypomineralization and caries formation; however, the mechanisms that initiate and lead to progression of tooth decay and periodontitis in type-1 DM have not been explored. To address this issue, we analyzed tooth morphology in Akita-/- mice that harbor a point mutation in the Ins2 insulin gene which leads to insulin deficiency and hyperglycemia. Objective: To characterize the tooth phenotype in Akita-/- mice and determine whether hyperglycemia and hyposalivation contribute to dental defects. Methods: Mandibles from Akita-/- and wild type littermates were analyzed by microCT, scanning EM and histology; teeth were examined for amelogenin and ameloblastin expression. Mice were injected ...
Solution-state NMR spectroscopy remains the primary method for characterising synthetic supramolecular assemblies. Yet, in their NMR spectra, relaxation interference effects can significantly alter peak intensities hindering interpretation. Here, we present a simple experiment for synthetic chemists to analy
Nanosphere, Inc. Statements of Operations (Unaudited) Three Month Periods Ended Nine Month Periods Ended September 30, September 30, ------------------------------------------------------ 2008 2007 2008 2007 ------------ ------------ ------------ ------------ (As Adjusted) (As Adjusted) REVENUE: Grant and contract revenue $ 73,459 $ 192,880 $ 346,071 $ 919,383 Product sales 209,772 6,125 707,891 59,795 ------------ ------------ ------------ ------------ Total revenue 283,231 199,005 1,053,962 979,178 COSTS AND EXPENSES: Cost of product sales 246,128 2,844 821,733 21,211 Research and development 5,683,346 5,778,946 17,631,003 16,287,056 Sales, general, and admini- strative 3,376,807 4,429,214 10,563,212 9,685,797 ------------ ------------ ------------ ------------ Total costs and expenses 9,306,281 10,211,004 29,015,948 25,994,064 ------------ ------------ ------------ ------------ Loss from operations (9,023,050) (10,011,999) (27,961,986) (25,014,886) OTHER INCOME (EXPENSE): Change in fair value ...
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The mineralized portions of teeth, the dentin and emel, are formed by mesenchyme-derived odontoblasts and epithelium-derived ameloblasts,…
Survival of the fittest: Self-assemblies made of dynamic block copolymers (dynablocks) can self-replicate by catalyzing the formation of their own building blocks. Moreover, in competition experiments, the differential thermodynamic stabilities and autocatalytic efficiencies of these self-assemblies lead to sigmoid growth of the most efficient self-replicator and to depletion of its competitors. ...
The construction of Cu(ii)-containing supramolecular chains is achieved by combining suitable anionic ligands (for controlling the coordination geometry and for creating a neutral building block) with four new bifunctional ligands containing a metal-coordinating pyridyl site and a self-complementary hydrogen
This week, .US has selected Nanosphere as the "Site of the Week." A great example of American-based scientific innovation at its core, Nanosphere is a molecular diagnostic platform that helps hospitals around the cou ... ...
Milyen meglep hajl konys g! Mert a l tsz lagos rendetlens g az rzelmi hull mz s bels l ktet s t k veti. A csal di f nyk pek, a k peken t l, sz leivel, testv reivel val hajdani viszony t id zi meg; a t jak, a v zpartok, a filmforgat sok eml keit. Egyszer azt nyilatkozta, hogy m r els filmje, a La Pointe Courte (1954) idej n, rz kenyen figyelt fel a dolgok ellent tes term szet re: „az egyik oldalon a f rfiak, a m sikon az asszonyok, a fekete s a feh r, a fa s a vas, a val s g vil ga, mely a megfigyelt emberek let b l sz lt, s a k pzelet vil ga, melyben a kalandoz szellem struktur kat s form kat tal l" - ez ragadta meg azonnal. Csakugyan, munk j ban nincs igazi k l nbs g re lis s fikcion lis, dokumentarista s meseszer k z tt. Gondoljunk a Sem fed l, sem t rv nyre, amely teljes eg sz ben „kital lt", megrendezett t rt net, bele rtve az interj kat, m gis text r j ban a dokumentumfilm vitathatatlan benyom s t kelti. Varda szereti keverni a k tf le m dszert, annyira, hogy sokszor t nyleg neh z ...
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A hegz r d st k vetoen vagy horzsol sokra- tod sekre hat kony h mregener ci t t mogat , hidrat l bioakt v komplex, amely az rz keny bor t pl l s ra szolg l s a bor megfelelo higi n s llapot nak kialak t s t seg ti.,Alkalmaz sa: V kony r tegben permete
Investigating the Properties of pH The manufactures claim is that Sensodyne toothpaste micro hardens tooth enamel to help protect against the effects of
I. Jolanda M. de Vries, W. Joost Lesterhuis, Nicole M. Scharenborg, Linda P. H. Engelen, Dirk J. Ruiter, Marie-Jeanne P. Gerritsen, Sandra Croockewit, Cedrik M. Britten, Ruurd Torensma, Gosse J. Adema, Carl G. Figdor and Cornelis J. A. Punt ...
Amelogenesis imperfecta (AI) is a group of inherited defects of dental enamel formation that show both clinical and genetic heterogeneity [1]. In its mildest form, AI causes discoloration, while in the most severe presentation the enamel is hypocalcified causing it to be abraded from the teeth shortly after their emergence into the mouth [2]. Both the primary and permanent dentitions may be affected. Enamel findings in AI are highly variable, ranging from deficient enamel formation to defects in the mineral and protein content [3]. Four main types of AI have been described: hypoplastic, hypocalcified, hypomaturation and hypomaturation-hypoplastic with taurodontism [4].. The AI phenotypes vary widely depending on the specific gene involved, the location and type of mutation, and the corresponding putative change at the protein level [5]. Different inheritance patterns such as X-linked, autosomal dominant and autosomal recessive types have been reported and 14 subtypes of AI are recognized ...
Amelogenesis imperfecta, type IV (AI4; MIM 104510) and trichodentoosseus syndrome (TDO; MIM 190320) are related disorders characterized by enamel hypoplasia and taurodontism. Tooth abnormalities are the only clinical features seen in individuals with AI4, while individuals with TDO also have hair, nail, and bone abnormalities. The additional findings seen in TDO may include dolichocephaly, kinky or curly hair, brittle nails, and a mild to moderate increase in bone density of the skull, spine and long bones. AI4 and TDO are autosomal dominant disorders caused by mutations in the DLX3 gene. DLX3 encodes the distal-less homeobox 3 protein, a transcription factor involved in regulation of gene expression during the development of various tissues.. Read less ...
im young female with 24 years old. i have an amelogenesis imperfecta symptoms, all of my teeth are small in size, yellow or brownish in color, and i have done 2 orthognathic surgeries and my third surgery was for tongue reduction. my surgries were difficult and so painful, but in the same time i like them because they help me to be better in my apperence ( prepare my jaws location). now im work on my denture ...
Our results suggest that some coding exons present in the coelacanth AMTN and in most of the tetrapods analyzed here derive from those of an ancestral SCPP, from which AMTN and EMPs originated after duplication. Indeed, AMTN exons 2a and 2b are homologous to exons 2 and 3 (exon 4 and 5 in ENAM) of all SCPPs, respectively. In addition, AMTN shares several other characteristics with all EMPs, i.e. the signal peptide encoded by exon 2a, the conserved Ser-X-Glu motif (putative phosphorylation site) encoded by exon 2b, and the presence of a variable region encoded by a large exon, as previously observed by Kawasaki and Weiss [6].. The presence of AMTN exon 2b in salamander cDNA and its absence in clawed frog, for which gDNA and cDNA sequences are available, indicates that this exon has been lost either early in the anuran lineage (no AMTN sequences available in other frog lineages), or later in the lineage leading to Pipidae (Figure 7). Exon 2b also lacks in mammalian AMTN, a finding indicating that ...
A range of regenerative treatments have been used for the treatment of periodontal infrabony defects. Guided tissue regeneration (GTR) and enamel matrix derivatives (EMD) are two popular treatment and they have both been used in combination with other treatment. This network meta-analysis was conducted to assess the potential effects of the various combinations. Unlike a [read the full story…]. ...
Build: Wed Jun 21 18:33:50 EDT 2017 (commit: 4a3b2dc). National Center for Advancing Translational Sciences (NCATS), 6701 Democracy Boulevard, Bethesda MD 20892-4874 • 301-435-0888. ...
that are essential for normal tooth development. These proteins are involved in the formation of enamel, which is the hard, calcium-rich material that forms the protective outer layer of each tooth. Mutations in any of these genes alter the structure of these proteins or prevent the genes from making any protein at all. As a result, tooth enamel is abnormally thin or soft and may have a yellow or brown color. Teeth with defective enamel are weak and easily damaged.[1] In some cases, the genetic cause of amelogenesis imperfecta can not been identified. Researchers are working to find mutations in other genes that are responsible for this disorder.[1]. Click on each gene name to learn more about the role it plays in the development of tooth enamel. ...
TY - JOUR. T1 - Evaluation of human recession defects treated with coronally advanced flaps and either enamel matrix derivative or connective tissue. Part 1. T2 - Comparison of clinical parameters. AU - McGuire, Michael K.. AU - Nunn, Martha E.. PY - 2003/8/1. Y1 - 2003/8/1. N2 - Background: Recession defects around teeth have been treated with a variety of surgical techniques. Most of the literature suggests that the subepithelial connective tissue graft has the highest percentage of mean root coverage with the least variability. Previous studies have demonstrated that enamel matrix derivative (EMD) has the ability to improve clinical parameters. The purpose of this study was to compare the clinical efficacy of enamel matrix derivative placed under a coronally advanced flap to subepithelial connective tissue placed under a coronally advanced flap in patients with recession type defects. Methods: Twenty patients with incisors or premolars presenting with a facial recession of ≥4 mm in ...
During the last few years several studies have been published dealing with the immunhistological properties of AOT. Immunohistochemically, the classical AOT phenotype is characterized by a cytokeratin (CK) profile similar to follicular cyst and/or oral or gingival epithelium based on positive staining with CK5, CK17 and CK19 [18]. On the other hand the classical AOT is negative for CK4, 10, 13 and 18. Recently, Crivelini et al. [19] detected the expression of cytokeratin 14 in AOT and concluded that this probably indicate its origin in the reduced dental epithelium which is also positive for staining with cytokeratin 14 antibodies. Positive reactions for amelogenin in limited areas in AOT are also reported as well as in ameloblasts and in the immature enamel matrix [20].. Interestingly, Takahashi et al. [21] observed a positive staining for iron-binding proteins (transferring, ferritin) and proteinase inhibitor (alpha-one-antitrypsin) in various cells of AOT indicating their role to the ...

leucine-rich amelogenin peptide
      - LRAP
     Summary Report | CureHunterleucine-rich amelogenin peptide - LRAP Summary Report | CureHunter

leucine-rich amelogenin peptide: translation product of an alternatively spliced transcript; amino acid sequence has been ... leucine-rich amelogenin peptide (LRAP). Subscribe to New Research on leucine-rich amelogenin peptide ...
more infohttp://www.curehunter.com/public/keywordSummaryC067432-leucine-rich-amelogenin-peptide-LRAP.do

Frontiers | Protein Phosphorylation and Mineral Binding Affect the Secondary Structure of the Leucine-Rich Amelogenin Peptide |...Frontiers | Protein Phosphorylation and Mineral Binding Affect the Secondary Structure of the Leucine-Rich Amelogenin Peptide |...

... and the Leucine-Rich Amelogenin Peptide (LRAP(+P)), an alternative amelogenin splice product, affects protein assembly and ... and the Leucine-Rich Amelogenin Peptide (LRAP(+P)), an alternative amelogenin splice product, affects protein assembly and ... Based on these findings, we hypothesize that the phosphorylation of full-length amelogenin and LRAP induces conformational ... Based on these findings, we hypothesize that the phosphorylation of full-length amelogenin and LRAP induces conformational ...
more infohttps://www.frontiersin.org/articles/10.3389/fphys.2017.00450/full

amelogenesis imperfecta type 1E Disease Ontology Browser - DOID:0110058amelogenesis imperfecta type 1E Disease Ontology Browser - DOID:0110058

Definition: An amelogenesis imperfecta that has material basis in mutation in the gene encoding amelogenin (AMELX). ...
more infohttp://www.informatics.jax.org/disease/301200

Exclusion of known gene for enamel development in two Brazilian families with amelogenesis imperfecta | Head & Face Medicine |...Exclusion of known gene for enamel development in two Brazilian families with amelogenesis imperfecta | Head & Face Medicine |...

Amelogenin, the protein product of the AMELX Xp22.3-p22.1 and AMELY Yp11 genes, is considered to be critical for normal enamel ... Additionally sequencing of amelogenin X gene did not reveal any mutations in this gene that could be associated with enamel ... Aldred MJ, Crawford PJ, Roberts E, Thomas NS: Identification of a nonsense mutation in the amelogenin gene (AMELX) in a family ... Collier PM, Sauk JJ, Rosenbloom SJ, Yuan ZA, Gibson CW: An amelogenin gene defect associated with human X-linked amelogenesis ...
more infohttps://head-face-med.biomedcentral.com/articles/10.1186/1746-160X-3-8

Amelogenin - WikipediaAmelogenin - Wikipedia

Although the precise role of amelogenin(s) in regulating the mineralization process is unknown, it is known that amelogenins ... "deleted-amelogenin males" (DAMs). In response the authors suggested that while the amelogenin sex test may be accurate in ... The amelogenin gene has been most widely studied in humans, where it is a single copy gene, located on the X and Y chromosomes ... Amelogenin is the name for a series of closely related proteins involved in amelogenesis, the development of enamel.[1] They ...
more infohttps://en.wikipedia.org/wiki/Amelogenin

WikiGenes - AMELX - amelogenin, X-linkedWikiGenes - AMELX - amelogenin, X-linked

Amelogenin dosage compensation in carcinoma of colon, lung, liver and kidney, is not a marker of clonality in males. Zvejnieks ... Amelogenin p.M1T and p.W4S mutations underlying hypoplastic X-linked amelogenesis imperfecta. Kim, J.W., Simmer, J.P., Hu, Y.Y ... The human enamel protein gene amelogenin is expressed from both the X and the Y chromosomes. Salido, E.C., Yen, P.H., ... Amelogenin cross-amplification in the family Bovidae and its application for sex determination. Weikard, R., Pitra, C., Kühn, C ...
more infohttps://www.wikigenes.org/e/gene/e/265.html

Amelogenin and enamel biomimetics - Journal of Materials Chemistry B (RSC Publishing)Amelogenin and enamel biomimetics - Journal of Materials Chemistry B (RSC Publishing)

Considering the importance of amelogenin protein in dental enamel formation, its ability to control apatite mineralization in ... Amelogenin and enamel biomimetics Q. Ruan and J. Moradian-Oldak, J. Mater. Chem. B, 2015, 3, 3112 DOI: 10.1039/C5TB00163C ... amelogenin and enamel biomimetics. We review the most recent findings on amelogenin secondary and tertiary structural ... Considering the importance of amelogenin protein in dental enamel formation, its ability to control apatite mineralization in ...
more infohttps://pubs.rsc.org/en/content/articlelanding/2015/tb/c5tb00163c/unauth

Amelogenin Y antibody | acris-antibodies.comAmelogenin Y antibody | acris-antibodies.com

Alternative names for Amelogenin Y antibody. AMELY, AMGL, AMGY, Amelogenin Y isoform ... Recombinant protein of human amelogenin, Y-linked (AMELY). Human. > 80 % Preparation: Recombint protein was captured through ... Background of Amelogenin Y antibody. Kit Component:. - KN212029G1, AMELY gRNA vector 1 in pCas-Guide vector. - KN212029G2, ...
more infohttps://www.acris-antibodies.com/target/amelogenin-y-antibody.htm

AMELX (amelogenin, X-linked) - KOMP (Knockout Mouse Project)AMELX (amelogenin, X-linked) - KOMP (Knockout Mouse Project)

Vega: OTTMUSG19595 (Amelx, amelogenin X chromosome)*CCDS: 28475.1, 41213, 41214*OMIM: AMELOGENIN; AMELX, AMELOGENESIS ... amelogenin, X-linked. Synonyms: Amel, Amg, M100888, Rgsc888. Gene nomenclature, locus information, and GO, OMIM, and PMID ...
more infohttps://www.komp.org/geneinfo.php?geneid=23206

Cementomimetics-constructing a cementum-like biomineralized microlayer via amelogenin-derived peptidesCementomimetics-constructing a cementum-like biomineralized microlayer via amelogenin-derived peptides

Using amelogenin as a case study and a bioinformatics scoring matrix, we identified regions within amelogenin that are shared ... One 22-amino acid long peptide regions referred to as amelogenin-derived peptide 5 (ADP5) was shown to facilitate cell-free ... "Cementomimetics-constructing a Cementum-like Biomineralized Microlayer via Amelogenin-derived Peptides." International Journal ...
more infohttps://kuscholarworks.ku.edu/handle/1808/18873

Frontiers | Mapping the Tooth Enamel Proteome and Amelogenin Phosphorylation Onto Mineralizing Porcine Tooth Crowns | PhysiologyFrontiers | Mapping the Tooth Enamel Proteome and Amelogenin Phosphorylation Onto Mineralizing Porcine Tooth Crowns | Physiology

Amelogenin (AMELX) with its splice variants is the most abundant enamel matrix protein, with only one known phosphorylation ... While AMELX regulates mineral transitions over space and time, it is unknown whether and when un-phosphorylated amelogenin ... While AMELX regulates mineral transitions over space and time, it is unknown whether and when un-phosphorylated amelogenin ... the shorter leucine-rich amelogenin protein (LRAP), and the exon 4-containing P190 in forming enamel, all within the context of ...
more infohttps://www.frontiersin.org/articles/10.3389/fphys.2019.00925/full

Characterization of the apical bridge barrier formed following amelogenin apexification | Springer for Research & DevelopmentCharacterization of the apical bridge barrier formed following amelogenin apexification | Springer for Research & Development

Background Recombinant amelogenin protein (RAP) is reported to induce complete root apex formation in dog model when used as ... Recombinant amelogenin RAP R-amelogenin Regeneration Apexification Root canal therapy Non-vital teeth Open apex Immature teeth ... A total of 240 root canals were divided into two groups: a recombinant amelogenin protein (180 amino acid mouse amelogenin) ( ... Amelogenin splicing isoforms, leucine-rich amelogenin peptide (LRAP), induces osteogenesis in various cell types by activating ...
more infohttps://rd.springer.com/article/10.1186%2Fs12903-018-0641-0

Putative signaling action of amelogenin utilizes the Wnt/β-catenin pathwayPutative signaling action of amelogenin utilizes the Wnt/β-catenin pathway

While it has long been known that amelogenin is essential for the proper development of enamel, its role has generally been ... In this article we report the successful purification of a recombinant mouse amelogenin protein and demonstrate that it has ... Using the TOPGAL transgenic mouse we showed that amelogenin expression in vivo is localized mainly around the root, the ... To determine the regulatory function of canonical Wnt signaling by amelogenin, we used TOPGAL transgenic mice. These mice ...
more infohttps://insights.ovid.com/jpre/200906000/00005201-200906000-00002

CiNii 論文 - 
 		
 		
 			
 		 	
 		 		
 		 			The effect of amelogenins (Xelma^|TM|) on hard-to-heal venous leg ulcers
 		 		
 	...CiNii 論文 - The effect of amelogenins (Xelma^|TM|) on hard-to-heal venous leg ulcers ...

The effect of amelogenins (Xelma^,TM,) on hard-to-heal venous leg ulcers * * VOWDEN Peter ...
more infohttps://ci.nii.ac.jp/naid/10018196758

KAKEN - Research Projects | The periodontal regeneration therapy with novel amelogenin binding protein Grp78 (KAKENHI-PROJECT...KAKEN - Research Projects | The periodontal regeneration therapy with novel amelogenin binding protein Grp78 (KAKENHI-PROJECT...

Presentation] Amelogenin Modulates Macrophage Phenotype During Inflammatory Responses.2016. *. Author(s). Kensuke Yamamichi, ... Presentation] Grp78 is crucial for cell migration induced by amelogenin in a human periodontal ligament cells.2016. *. Author(s ... Presentation] Grp78 Is Critical For Amelogenin-induced Cell Migration In PDLSCs2015. *. Author(s). Takao Fukuda, Kyosuke Toyoda ... Presentation] Molecular basis of amelogenin-induced periodontal tissue regeneration2016. *. Author(s). Takao Fukuda, Terukazu ...
more infohttps://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15H06492/

MMP-20 hydrolysis of amelogenin in solution (AMG-S) and | Open-iMMP-20 hydrolysis of amelogenin in solution (AMG-S) and | Open-i

MMP-20 hydrolysis of amelogenin in solution (AMG-S) and bound on HAP (AMG-HAP) were digested and quantified by HPLC. (A) HPLC ... B) Quantified UV areas of 25 kDa amelogenin substrate after digestion. (C) Quantified UV areas of 23 kDa amelogenin derivative ... B) Quantified UV areas of 25 kDa amelogenin substrate after digestion. (C) Quantified UV areas of 23 kDa amelogenin derivative ... The results show that as compared with amelogenin in solution, the HAP-bound amelogenin was hydrolyzed by both MMP20 and KLK4 ...
more infohttps://openi.nlm.nih.gov/detailedresult.php?img=PMC4109566_fphys-05-00268-g0003&req=4

Amelogenin in cranio-facial development: the tooth as a model to study the role of amelogenin during embryogenesis  - Zurich...Amelogenin in cranio-facial development: the tooth as a model to study the role of amelogenin during embryogenesis - Zurich...

Amelogenin in cranio-facial development: the tooth as a model to study the role of amelogenin during embryogenesis ... Download PDF Amelogenin in cranio-facial development: the tooth as a model to study the role of amelogenin during ... Amelogenin in cranio-facial development: the tooth as a model to study the role of amelogenin during embryogenesis. Journal of ... Recently, some amelogenin isoforms were found to bind to the cell surface receptors; LAMP-1, LAMP-2 and CD63, and subsequently ...
more infohttps://www.zora.uzh.ch/id/eprint/9207/

Gene expression in tooth: amelogeninGene expression in tooth: amelogenin

Expression of amelogenin in mouse tooth. Amelx; Algn; Amg; Amgl. Species: mouse Location in mouse genome: chromosome X, ... Mutations in amelogenin gene cause X-linked amelogenesis imperfecta.. Source references: Snead,M.L., Luo,W., Lau,E.C., and, ...
more infohttp://bite-it.helsinki.fi/AGENIN.HTM

Comment on Sex estimation using sexually dimorphic amelogenin protein fragments in human enamelComment on Sex estimation using sexually dimorphic amelogenin protein fragments in human enamel

Amelogenin genes are located on both X and Y sex chromosomes in humans and are a major focus of DNA-based sex estimation ... Amelogenin proteins, AMELX_HUMAN and AMELY_HUMAN, are expressed in the tooth organ and play a major role in mineralization of ... Parker, G.J., et al Sex estimation using sexually dimorphic amelogenin protein fragments in human enamel. Journal of ... Sex estimation using sexually dimorphic amelogenin protein fragments in human enamel. 00 ...
more infohttp://www.bioinfor.com/sex-estimation-using-sexually-dimorphic-amelogenin-protein-fragments-human-enamel/

Ex Vivo Study of the Angiogenic Effect of the Extracellular Matrix Protein AmelogeninEx Vivo Study of the Angiogenic Effect of the Extracellular Matrix Protein Amelogenin

Amelogenin was added (0.01 mg/ml, 0.1 mg/ml and 1 mg/ml) in serum-free endothelial basal growth medium. Porcine serum albumin ... Amelogenin is an extracellular matrix protein that has been reported to promote formation of granulation tissue and repair of ... Results and Discussion: Amelogenin at 0.1 mg/ml significantly (p = 0.001) increased micro-vessel outgrowth by 76 % from the ... The preliminary data from the integrin assay show that amelogenin at 0.1 mg/ml also displays a broad up-regulation of several ...
more infohttps://research.chalmers.se/publication/81481

Biomimetic Tooth Repair: Amelogenin-Derived Peptide Enables in Vitro Remineralization of Human Enamel - Peer Effects Inc.Biomimetic Tooth Repair: Amelogenin-Derived Peptide Enables in Vitro Remineralization of Human Enamel - Peer Effects Inc.

Biomimetic Tooth Repair: Amelogenin-Derived Peptide Enables in Vitro Remineralization of Human Enamel. Home/Biomimetic Tooth ... Biomimetic Tooth Repair: Amelogenin-Derived Peptide Enables in Vitro Remineralization of Human Enamel. Posted by PeerEffects ... This study demonstrates that small peptide domains derived from native protein amelogenin can be utilized to construct a ...
more infohttps://peereffects.com/blog/dentist/biomimetic-tooth-repair-amelogenin-derived-peptide-enables-in-vitro-remineralization-of-human-enamel/

Structural variation on the short arm of the human Y chromosome: recurrent multigene deletions encompassing Amelogenin YStructural variation on the short arm of the human Y chromosome: recurrent multigene deletions encompassing Amelogenin Y

Assay of the Amelogenin Y gene (AMELY) on Yp is widely used in DNA-based sex testing, and sometimes reveals males who have ... Structural variation on the short arm of the human Y chromosome: recurrent multigene deletions encompassing Amelogenin Y ...
more infohttps://scholars.latrobe.edu.au/display/publication25868

Amelogenin Peptide Extract Increases Differentiation and Angiogenic an by Rene Olivares-Navarrete, Sharon L. Hyzy et al."Amelogenin Peptide Extract Increases Differentiation and Angiogenic an" by Rene Olivares-Navarrete, Sharon L. Hyzy et al.

However, the role of amelogenin or amelogenin peptides in mediating osteoblast response to EMD is not clear. Human MG63 ... Both amelogenin and 5 kDa peptides protected MG63s from chelerythrine-induced apoptosis. The data suggest that the 5 kDa TRAP- ... Amelogenin, EMDs principal component, spontaneously assembles into nanospheres in vivo, forming an ECM complex that releases ... cells or normal human osteoblasts were treated with recombinant human amelogenin or a 5 kDa tyrosine-rich amelogenin peptide ( ...
more infohttps://scholarscompass.vcu.edu/egrb_pubs/14/

q99218-2 - purathamq99218-2 - puratham

Amelogenin. Amelogenin superfamily. Home. This webpage was best viewed in Mozilla Firefox 3.05 ...
more infohttps://sites.google.com/site/puratham/q99218-2
  • Based on these findings, we hypothesize that the phosphorylation of full-length amelogenin and LRAP induces conformational changes that critically affect its capacity to interact with forming calcium phosphate mineral phases. (frontiersin.org)
  • These collective findings indicate that phosphorylation induces unique secondary structural changes that may enhance the functional capacity of native phosphorylated amelogenins like LRAP to stabilize an ACP precursor phase during early stages of enamel mineral formation. (frontiersin.org)
  • Developing human enamel contains about 70% protein, 90% of which are amelogenins. (wikipedia.org)
  • Considering the importance of amelogenin protein in dental enamel formation, its ability to control apatite mineralization in vitro , and its potential to be applied in fabrication of future bio-inspired dental material this review focuses on two major subjects: amelogenin and enamel biomimetics. (rsc.org)
  • This study aims to reveal the spatiotemporal distribution of the cleavage products of the most abundant AMLEX splice variants including the full length P173, the shorter leucine-rich amelogenin protein (LRAP), and the exon 4-containing P190 in forming enamel, all within the context of the changing enamel matrix proteome during mineralization. (frontiersin.org)
  • Recombinant amelogenin protein (RAP) is reported to induce complete root apex formation in dog model when used as apexification therapy. (springer.com)
  • In this article we report the successful purification of a recombinant mouse amelogenin protein and demonstrate that it has signaling activity in isolated mouse calvarial cells and human periodontal ligament cells. (ovid.com)
  • Our recent proteomic study identified glucose-regulated protein 78 (Grp78) as a new amelogenin-binding protein. (nii.ac.jp)
  • The hardest tooth enamel tissue develops from a soft layer of protein-rich matrix, predominated by amelogenin that is secreted by epithelial ameloblasts in the secretory stage of tooth enamel development. (nih.gov)
  • Equal quantities of amelogenins confirmed by protein assays before digestions, either adsorbed to HAP or in solution, were incubated with MMP20 or KLK4. (nih.gov)
  • As shown in Figure 3B, the full-length amelogenin in solution was hydrolyzed significantly slower than the protein attached to the crystal surface. (nih.gov)
  • Amelogenin protein expression was detected in the tooth germ (dental lamina) already at E13.5, much earlier than previously reported (E19). (uzh.ch)
  • The present results indicate that amelogenin has an important role in many tissues of the cranio-facial complex during mouse embryonic development and differentiation, and might be a multifunctional protein. (uzh.ch)
  • While the signal of each gene product can vary by more than an order of magnitude, we show close agreement between osteological and amelogenin-based sex estimation and thus demonstrate that the protein-based signal can be obtained reliably from open-air archaeological contexts dating to at least 7300 years ago. (bioinfor.com)
  • Amelogenin is an extracellular matrix protein that has been reported to promote formation of granulation tissue and repair of chronic venous leg ulcers and elevate the pro-angiogenic vascular endothelial growth factor in dermal fibroblasts.1-3 This study investigated the effect of amelogenin on angiogenesis in an ex vivo sprouting assay and related the findings to the cell surface integrin expression. (chalmers.se)
  • This study demonstrates that small peptide domains derived from native protein amelogenin can be utilized to construct a mineral layer on damaged human enamel in vitro. (peereffects.com)
  • Another study in India, however, found 5 of its 270 men studied (1.85%) possessed an AMELY deletion, terming them "deleted-amelogenin males" (DAMs). (wikipedia.org)
  • Assay of the Amelogenin Y gene (AMELY) on Yp is widely used in DNA-based sex testing, and sometimes reveals males who have interstitial deletions. (edu.au)
  • In these in vitro models, we showed that amelogenin can activate β-catenin signaling. (ovid.com)
  • Preferential and selective degradation and removal of amelogenin adsorbed on hydroxyapatites by MMP20 and KLK4 in vitro. (nih.gov)
  • These results suggest that the adsorption of amelogenin to HAP results in their preferential and selective degradation and removal from HAP by MMP20 and KLK4 in vitro. (nih.gov)
  • The amelogenin gene has been most widely studied in humans, where it is a single copy gene, located on the X and Y chromosomes at Xp22.1-Xp22.3 and Yp 11.2 . (wikipedia.org)
  • Is the amelogenin gene reliable for gender identification in forensic casework and prenatal diagnosis? (wikipedia.org)
  • To investigate in greater detail the molecular mechanisms involved in the β-catenin signaling pathway, isolated osteoblasts and periodontal ligament cells were exposed to full-length recombinant mouse amelogenin and were evaluated for phenotypic changes and β-catenin signaling using a TOPFLASH construct and the LacZ reporter gene. (ovid.com)
  • Using amelogenin as a case study and a bioinformatics scoring matrix, we identified regions within amelogenin that are shared with a set of hydroxyapatite-binding peptides (HABPs) previously selected by phage display. (ku.edu)
  • Using the TOPGAL transgenic mouse we showed that amelogenin expression in vivo is localized mainly around the root, the periodontal ligament and the alveolar bone. (ovid.com)
  • Journal Article] Grp78 is Critical for Amelogenin-Induced Cell Migration in a Multipotent Clonal Human Periodontal Ligament Cell Line. (nii.ac.jp)
  • Presentation] Grp78 is crucial for cell migration induced by amelogenin in a human periodontal ligament cells. (nii.ac.jp)
  • Integrin assay: Human dermal microvascular endothelial cells (Promocell) were seeded in complete cell growth medium alone or supplemented with 0.1 mg/ml, 1 mg/ml amelogenin or 20 µg/ml fibronectin. (chalmers.se)
  • The preliminary data from the integrin assay show that amelogenin at 0.1 mg/ml also displays a broad up-regulation of several integrins/subunits. (chalmers.se)
  • Conclusions: Amelogenin stimulated micro-vessel outgrowth in the chick aortic arch assay possibly through up-regulation of several integrins and subunits important for cell interaction with the ECM. (chalmers.se)
  • Application of amelogenin (rHAM(+)) beads together with DiI, on E13.5 and E14.5 embryonic mandibular mesenchyme and on embryonic tooth germ, revealed recruitment of mesenchymal cells. (uzh.ch)
  • Interestingly, we note that the 23 kDa amelogenin fragment rapidly increases in the first hour of the digestion. (nih.gov)
  • we demonstrate the cellular responses induced by the biological interaction between amelogenin and Grp78 in the human undifferentiated PDL cell line 1-17, which possesses the most typical characteristics of PDLSCs. (nii.ac.jp)
  • In addition, amelogenins appear to aid in the development of cementum by directing cementoblasts to the tooth's root surface. (wikipedia.org)
  • Using LC-MALDI MS/MS, more accessible cleavage sites and hydrolytic fragments from MMP20/KLK4 digestion were identified for the amelogenin adsorbed on HAP crystals as compared to the amelogenin in solution. (nih.gov)
  • The results show that as compared with amelogenin in solution, the HAP-bound amelogenin was hydrolyzed by both MMP20 and KLK4 at significantly higher rates. (nih.gov)
  • Results and Discussion: Amelogenin at 0.1 mg/ml significantly (p = 0.001) increased micro-vessel outgrowth by 76 % from the explants compared with control explants after 48 h of incubation. (chalmers.se)
  • Amelogenin genes are located on both X and Y sex chromosomes in humans and are a major focus of DNA-based sex estimation methods. (bioinfor.com)
  • Taken together, the present observations suggest that the angiogenic effects might be explained by the cell binding properties of amelogenin. (chalmers.se)
  • These results suggest that Grp78 is essential for enhancing amelogenin-induced migration in 1-17 cells. (nii.ac.jp)
  • The results of HPLC demonstrated that the digestion of amelogenin on HAP or in solution immediately produced one product peak (labeled "23 kDa" in Figure 3A). (nih.gov)