Ambystoma mexicanum: A salamander found in Mexican mountain lakes and accounting for about 30 percent of the urodeles used in research. The axolotl remains in larval form throughout its life, a phenomenon known as neoteny.Ambystoma: A genus of the Ambystomatidae family. The best known species are the axolotl AMBYSTOMA MEXICANUM and the closely related tiger salamander Ambystoma tigrinum. They may retain gills and remain aquatic without developing all of the adult characteristics. However, under proper changes in the environment they metamorphose.Urodela: An order of the Amphibia class which includes salamanders and newts. They are characterized by usually having slim bodies and tails, four limbs of about equal size (except in Sirenidae), and a reduction in skull bones.Pleurodeles: A genus of aquatic newts belonging to the family Salamandridae and sometimes referred to as "spiny" tritons. There are two species P. waltlii and P. poireti. P. waltlii is commonly used in the laboratory. Since this genus adapts to aquarium living, it is easy to maintain in laboratories.Regeneration: The physiological renewal, repair, or replacement of tissue.Parabiosis: The experimental joining of two individuals for the purpose of studying the effects of one on the other.Extremities: The farthest or outermost projections of the body, such as the HAND and FOOT.Forelimb: A front limb of a quadruped. (The Random House College Dictionary, 1980)Larva: Wormlike or grublike stage, following the egg in the life cycle of insects, worms, and other metamorphosing animals.Amphibians: VERTEBRATES belonging to the class amphibia such as frogs, toads, newts and salamanders that live in a semiaquatic environment.Triturus: A genus of aquatic newts in the Salamandridae family. During breeding season many Triturus males have a dorsal crest which also serves as an accessory respiratory organ. One of the common Triturus species is Triturus cristatus (crested newt).Indigo Carmine: Indolesulfonic acid used as a dye in renal function testing for the detection of nitrates and chlorates, and in the testing of milk.BooksRanunculus: A plant genus of the family RANUNCULACEAE that contains protoanemonin, anemonin, and ranunculin.Endoderm: The inner of the three germ layers of an embryo.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Homozygote: An individual in which both alleles at a given locus are identical.Catastrophic Illness: An acute or prolonged illness usually considered to be life-threatening or with the threat of serious residual disability. Treatment may be radical and is frequently costly.Dental Staff, Hospital: Dental personnel practicing in hospitals.Encyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)Temperature: The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.Tigers: The species Panthera tigris, a large feline inhabiting Asia. Several subspecies exist including the Siberian tiger and Sumatran tiger.Iguanas: Large herbivorous tropical American lizards.Oxytricha: A genus of ciliate protozoa having a unique cursorial type of locomotion.Macronucleus: The larger of two types of nuclei in ciliate protozoans. It is the transcriptionally active nucleus of the vegetative cells as distinguished from the smaller transcriptionally inert GERMLINE MICRONUCLEUS.Saint Vincent and the Grenadines: A self-governing state of the Windward Islands in the West Indies, comprising Saint Vincent and the northern islets of the Grenadines. Its capital is Kingstown. It is one of the original homes of the Carib Indians supposed to have been sighted by Columbus in 1498. It was in English hands from 1627 till held by the French 1779-83. Saint Vincent subsequently became a British possession and, with other nearby British territories, was administered by the Governor of the Windward Islands till 1959. It attained a measure of independence in 1969 but achieved full independence as Saint Vincent and the Grenadines in 1979. Saint Vincent was the 4th century Spanish martyr on whose feast day Columbus discovered the island. Grenadines is derived from the Spanish kingdom of Granada. (From Webster's New Geographical Dictionary, 1988, p1054 & The Europa World Year Book 1993, p2441)Famous PersonsMicronucleus, Germline: The smaller, reproductive, transcriptionally inert nucleus in the cells of ciliate protozoans, as distinguished from the larger, vegetative, transcriptionally active MACRONUCLEUS. Micronuclei participate in MEIOSIS and autogamy during GENETIC CONJUGATION.Genome, Protozoan: The complete genetic complement contained in a set of CHROMOSOMES in a protozoan.Fishes: A group of cold-blooded, aquatic vertebrates having gills, fins, a cartilaginous or bony endoskeleton, and elongated bodies covered with scales.Ischium: One of three bones that make up the coxal bone of the pelvic girdle. In tetrapods, it is the part of the pelvis that projects backward on the ventral side, and in primates, it bears the weight of the sitting animal.Ilium: The largest of three bones that make up each half of the pelvic girdle.Spheniscidae: The sole family in the order Sphenisciformes, comprised of 17 species of penguins in six genera. They are flightless seabirds of the Southern Hemisphere, highly adapted for marine life.Reproduction: The total process by which organisms produce offspring. (Stedman, 25th ed)Longevity: The normal length of time of an organism's life.Buffers: A chemical system that functions to control the levels of specific ions in solution. When the level of hydrogen ion in solution is controlled the system is called a pH buffer.Fetus: The unborn young of a viviparous mammal, in the postembryonic period, after the major structures have been outlined. In humans, the unborn young from the end of the eighth week after CONCEPTION until BIRTH, as distinguished from the earlier EMBRYO, MAMMALIAN.Energy Metabolism: The chemical reactions involved in the production and utilization of various forms of energy in cells.Baseball: A competitive nine-member team sport including softball.Reproduction, Asexual: Reproduction without fusion of two types of cells, mostly found in ALGAE; FUNGI; and PLANTS. Asexual reproduction occurs in several ways, such as budding, fission, or splitting from "parent" cells. Only few groups of ANIMALS reproduce asexually or unisexually (PARTHENOGENESIS).Genetic Linkage: The co-inheritance of two or more non-allelic GENES due to their being located more or less closely on the same CHROMOSOME.Chromosome Mapping: Any method used for determining the location of and relative distances between genes on a chromosome.Genetic Markers: A phenotypically recognizable genetic trait which can be used to identify a genetic locus, a linkage group, or a recombination event.Veterinary Medicine: The medical science concerned with the prevention, diagnosis, and treatment of diseases in animals.Netherlands: Country located in EUROPE. It is bordered by the NORTH SEA, BELGIUM, and GERMANY. Constituent areas are Aruba, Curacao, Sint Maarten, formerly included in the NETHERLANDS ANTILLES.Education, Veterinary: Use for general articles concerning veterinary medical education.NevadaSchools, Veterinary: Educational institutions for individuals specializing in the field of veterinary medicine.Animal Testing Alternatives: Procedures, such as TISSUE CULTURE TECHNIQUES; mathematical models; etc., when used or advocated for use in place of the use of animals in research or diagnostic laboratories.Coniferophyta: A plant division of GYMNOSPERMS consisting of cone-bearing trees and shrubs.Petromyzon: A genus of primitive fish in the family Petromyzontidae. The sole species is Petromyzon marinus, known as the sea lamprey. The adult form feeds parasitically on other fish species.Dromaiidae: A family of flightless, running BIRDS, in the order Casuariiformes. The emu is the only surviving member of the family. They naturally inhabit forests, open plains, and grasslands in Australia.Lampreys: Common name for the only family (Petromyzontidae) of eellike fish in the order Petromyzontiformes. They are jawless but have a sucking mouth with horny teeth.Angiotensin I: A decapeptide that is cleaved from precursor angiotensinogen by RENIN. Angiotensin I has limited biological activity. It is converted to angiotensin II, a potent vasoconstrictor, after the removal of two amino acids at the C-terminal by ANGIOTENSIN CONVERTING ENZYME.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.

Modulation of gelatinase activity correlates with the dedifferentiation profile of regenerating salamander limbs. (1/189)

Remodeling of extracellular matrix (ECM) is one of the key events in many developmental processes. In the present study, a temporal profile of gelatinase activities in regenerating salamander limbs was examined zymographically. In addition, the effect of retinoic acid (RA) on these enzyme activities was examined to relate the pattern-duplicating effect of RA in limb regenerates with gelatinase activities. During regeneration, various types of gelatinase activities were detected, and these activities were at their maximum levels at the dedifferentiation stage. Upon treatment with chelating agents EDTA and 1,10-phenanthroline, the enzyme activities were inhibited indicating that those enzymes are likely matrix metalloproteinases (MMPs). Considering the molecular sizes and the decrease of molecular sizes by treatment with p-aminophenylmercuric acetate, an artificial activator of proMMP, some of the gelatinases expressed during limb regeneration are presumed to be MMP-2 and MMP-9. In RA-treated regenerates, overall gelatinase activities increased, especially the MMP-2-like gelatinase activity which increased markedly. These results suggest that MMP-2-like and MMP-9-like gelatinases play a role in ECM remodeling during regeneration, and that gelatinases are involved in the excessive dedifferentiation after RA treatment.  (+info)

The cardiac neural crest in Ambystoma mexicanum. (2/189)

To establish whether a region of the cranial neural crest contributes cells to the developing heart of Ambystoma mexicanum (axolotl), as it does in many other vertebrates, we constructed a fate map for the neural crest in late neurula stage (stage 19-20) embryos. The fluorescent vital dye, Dil, was used as the lineage label. The various regions of the cranial neural folds were identified in relation to such landmarks as the developing forebrain, midbrain and hindbrain, and the appearance and extent of emerging somites. Labelled cells originating in the rhombencephalic region were found in the aortic arches and in the truncus arteriosus, and occasionally in the walls of the conus arteriosus. Cells were also found in the third and fourth branchial arches. Labelled neural crest from the adjacent anterior trunk region appeared neither in the heart nor the visceral skeleton, whereas those from the mesencephalic region contributed to the first hypobranchial cartilage and to the first three branchial arches, but not to the heart. No labelled cells from any of the regions were seen in the ventricle or auricle.  (+info)

Analysis of cranial neural crest migratory pathways in axolotl using cell markers and transplantation. (3/189)

We have examined the ability of normal and heterotopically transplanted neural crest cells to migrate along cranial neural crest pathways in the axolotl using focal DiI injections and in situ hybridization with the neural crest marker, AP-2. DiI labeling demonstrates that cranial neural crest cells migrate as distinct streams along prescribed pathways to populate the maxillary and mandibular processes of the first branchial arch, the hyoid arch and gill arches 1-4, following migratory pathways similar to those observed in other vertebrates. Another neural crest marker, the transcription factor AP-2, is expressed by premigratory neural crest cells within the neural folds and migrating neural crest cells en route to and within the branchial arches. Rotations of the cranial neural folds suggest that premigratory neural crest cells are not committed to a specific branchial arch fate, but can compensate when displaced short distances from their targets by migrating to a new target arch. In contrast, when cells are displaced far from their original location, they appear unable to respond appropriately to their new milieu such that they fail to migrate or appear to migrate randomly. When trunk neural folds are grafted heterotopically into the head, trunk neural crest cells migrate in a highly disorganized fashion and fail to follow normal cranial neural crest pathways. Importantly, we find incorporation of some trunk cells into branchial arch cartilage despite the random nature of their migration. This is the first demonstration that trunk neural crest cells can form cartilage when transplanted to the head. Our results indicate that, although cranial and trunk neural crest cells have inherent differences in ability to recognize migratory pathways, trunk neural crest can differentiate into cranial cartilage when given proper instructive cues.  (+info)

Cloning of cDNAs encoding retinoic acid receptors RAR gamma 1, RAR gamma 2, and a new splicing variant, RAR gamma 3, from Aambystoma mexicanum and characterization of their expression during early development. (4/189)

To analyze retinoic acid (RA) receptor (RAR) expression during early development in the urodele embryo, we have isolated cDNAs for four members of the axolotl (Ambystoma mexicanum) RAR family, namely RAR alpha (NR1B1), aRAR gamma 1 (NR1B3a), aRAR gamma 2 (NR1B3b), and a new splicing variant of aRAR gamma 2, aRAR gamma 3 (NR1B3c), which contains an insertion of five hydrophobic amino acids in the C-terminal region of the DNA binding domain. The temporal expression pattern of the RAR gamma isoforms was established by RT-PCR using total RNA from embryos of different stages. The expression of aRAR gamma 2 coincides with neurulation and is enhanced in the extremities of the embryo's anteroposterior axis. The aRAR gamma 3 is specifically expressed during gastrulation and early neurulation, whereas aRAR gamma 1 is expressed later during organogenesis. Global aRAR gamma 2 mRNA levels, as well as their spatio-temporal expression pattern in the neurula, were not affected by treatment with RA. These results show that several RARs are expressed in the axolotl embryo during early development, and reveal the existence of a new RAR gamma variant.  (+info)

GDNF and GFRalpha-1 are components of the axolotl pronephric duct guidance system. (5/189)

In mammals, secretion of GDNF by the metanephrogenic mesenchyme is essential for branching morphogenesis of the ureteric bud and, thus, metanephric development. However, the expression pattern of GDNF and its receptor complex-the GPI-linked ligand-binding protein, GFRalpha-1, and the Ret tyrosine kinase signaling protein-indicates that it could operate at early steps in kidney development as well. Furthermore, the developing nephric systems of fish and amphibian embryos express components of the GDNF signaling system even though they do not make a metanephros. We provide evidence that GDNF signaling through GFRalpha-1 is sufficient to direct pathfinding of migrating pronephric duct cells in axolotl embryos by: (1) demonstrating that application of soluble GFRalpha-1 to an embryo lacking all GPI-linked proteins rescues PND migration in a dose-dependent fashion, (2) showing that application of excess soluble GFRalpha-1 to a normal embryo inhibits migration and that inhibition is dependent upon GDNF-binding activity, and (3) showing that the PND will migrate toward a GDNF-soaked bead in vivo, but will fail to migrate when GDNF is applied uniformly to the flank. These data suggest that PND pathfinding is accomplished by migration up a gradient of GDNF.  (+info)

Expression and characterization of fibroblast growth factor 8 from Mexican axolotl, Ambystoma mexicanum. (6/189)

Fibroblast growth factor (FGF) has been known to regulate the proliferation and differentiation of a variety of cell types via interaction with a specific FGF receptor on the cell surface. In the present study, Fgf8 cDNA of Mexican axolotl, Ambystoma mexicanum, was expressed in Escherichia coli as an MBP-FGF8 fusion protein. The cell proliferation activity of the recombinant FGF8 (rFGF8) was measured by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazoliumbromide (MTT) assay. The addition of rFGF8 to the culture medium enhanced proliferation of BALB/c 3T3 and BHK21 cells about 1.4-1.5 fold. To analyze the binding activity of rFGF8 to the cell surface, cell surface enzyme linked immunosorbent assay was developed. Comparison of the structure of basic FGF with the computer-simulated structure of FGF8 suggested that Tyr-58, Glu-132, Tyr-139, and Leu-179 might be the potential receptor binding sites. Amino acid substitution muteins of FGF8 were constructed by PCR-derived directed mutagenesis and the muteins were overexpressed in E. coli. The rFGF8 muteins were purified and their binding activities were analyzed. Substitution of Tyr-58 or Glu-132 or Leu-179 of the FGF8 with alanine reduced the binding affinity, while substitution of Tyr-139 with alanine did not alter the binding affinity. These results imply that Tyr-58, Glu-132, and Leu-179 of FGF8 might be involved in its binding to the cell surface.  (+info)

Different regulation of T-box genes Tbx4 and Tbx5 during limb development and limb regeneration. (7/189)

The T-domain transcription factors Tbx4 and Tbx5 have been implicated, by virtue of their limb-type specific expression, in controlling the identity of vertebrate legs and arms, respectively. To study the roles of these genes in developing and regenerating limbs, we cloned Tbx4 and Tbx5 cDNAs from the newt, and generated antisera that recognize Tbx4 or Tbx5 proteins. We show here that, in two urodele amphibians, newts and axolotls, the regulation of Tbx4 and Tbx5 differs from higher vertebrates. At the mRNA and protein level, both Tbx4 and Tbx5 are expressed in developing hindlimbs as well as in developing forelimbs. The coexpression of these genes argues that additional factors are involved in the control of limb type-specific patterns. In addition, newt and axolotl Tbx4 and Tbx5 expression is regulated differently during embryogenesis and regenerative morphogenesis. During regeneration, Tbx5 is exclusively upregulated in the forelimbs, whereas Tbx4 is exclusively upregulated in the hindlimbs. This indicates that, on a molecular level, different regulatory mechanisms control the shaping of identical limb structures and that regeneration is not simply a reiteration of developmental gene programs.  (+info)

Extending the table of stages of normal development of the axolotl: limb development. (8/189)

The existing table of stages of the normal development of the axolotl (Ambystoma mexicanum) ends just after hatching. At this time, the forelimbs are small buds. In this study, we extend the staging series through completion of development of the forelimbs and hindlimbs.  (+info)

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Background: The fish-tetrapod transition was one of the major events in vertebrate evolution and was enabled by many morphological changes. Although the transformation of paired fish fins into tetrapod limbs has been a major topic of study in recent years, both from paleontological and comparative developmental perspectives, the interest has focused almost exclusively on the distal part of the appendage and in particular the origin of digits. Relatively little attention has been paid to the transformation of the pelvic girdle from a small unipartite structure to a large tripartite weight-bearing structure, allowing tetrapods to rely mostly on their hindlimbs for locomotion. In order to understand how the ischium and the ilium evolved and how the acetabulum was reoriented during this transition, growth series of the Australian lungfish Neoceratodus forsteri and the Mexican axolotl Ambystoma mexicanum were cleared and stained for cartilage and bone and immunostained for skeletal muscles. In order ...
The fish-tetrapod transition was one of the major events in vertebrate evolution and was enabled by many morphological changes. Although the transformation of paired fish fins into tetrapod limbs has been a major topic of study in recent years, both from paleontological and comparative developmental perspectives, the interest has focused almost exclusively on the distal part of the appendage and in particular the origin of digits. Relatively little attention has been paid to the transformation of the pelvic girdle from a small unipartite structure to a large tripartite weight-bearing structure, allowing tetrapods to rely mostly on their hindlimbs for locomotion. In order to understand how the ischium and the ilium evolved and how the acetabulum was reoriented during this transition, growth series of the Australian lungfish Neoceratodus forsteri and the Mexican axolotl Ambystoma mexicanum were cleared and stained for cartilage and bone and immunostained for skeletal muscles. In order to understand the
Dinoflagellates collected during red tide events in Bahia Mazatlan, Mexico during the early spring of 1999 and 2000 appeared under LM to belong to Prorocentrum mexicanum Osorio-Tafall. Observations with SEM of those populations showed marked differences in shape and microornamentation from the related species, Prorocentrum rhathymum Loeblich III, Sherley and Schmidt. In P. mexicanum, the presence and dimensions of poroids, the uneven distribution of trichocyst pores not located in depressions, and the general architecture of the periflagellar region are more closely related to Prorocentrum caribbaeum Faust. Also, P. mexicanum has a three-horned (sometimes two-horned) spine and is deeper in the anterior than the posterior region, whereas P. rhathymum has a simple small spine and its sagittal view is oval. Furthermore, the number and distribution of trichocyst pores in the periflagellar area is different between the two species, being located on both valves in P. mexicanum and only on the right ...
Molecular Analysis of Tropomyosin Deficiency in Mutant Axolotl Hearts. The long term goal of our laboratory is to elucidate the cellular and molecular mechanisms that regulate embryonic and post-embryonic cardiac development. The cardiac lethal mutation in the Mexican axolotl (Ambystoma mexicanum) has proven a useful tool for studying heart development in vertebrates. The naturally occurring mutation was discovered in 1968 by R. R. Humphrey in a dark stock of axolotls brought to the United States from Mexico. The cardiac non- function phenotype is a simple homozygous recessive mutation that results in failure of the affected hearts to beat. Mutant axolotl embryos are obtained from matings of heterozygous (+/c x +/c) parents, with twenty-five percent of the progeny expressing the mutant gene.. Electron microscopic analysis has demonstrated that the mutant axolotl hearts do not beat because they lack organized sarcomeres. In other words, the mutant hearts lack the contractile apparatus necessary ...
It was a pleasant suprise when i first saw my leucistic axolotls spawning a few weeks ago (especially since i got them as two females), and after eggs being laid, and 18-22 days of incubation, I have hatchlings ...
The premature death (p) mutation is a recessive lethal, which, in the homozygous condition, gives rise to a complex of abnormalities. The mutant embryos develop only to stage 37, at which time disintegration of superficial tissue begins. Many of the abnormalities observed in sections of the stage-37 mutant embryo are related to its failure to establish a functioning circulatory system, or to the resulting edema and/or ascites that distend the abdomen and flanks. There are, however, abnormalities of heart, liver, gill and muscle development which cannot be attributed to lack of circulation and edema. All of these abnormalities can be indirectly related to the endoderm, particularly the anterior and dorsal endoderm. The findings, therefore, suggest that the mutation leads to a fairly general defect of the endoderm.. ...
So, I recently lost my entire tank (save for my pleco) to a catastrophic event of unknown cause. Ive gotten over it now and am ready to start anew. This time
BACKGROUND: The Mexican axolotl (Ambystoma mexicanum) is considered a hopeful monster because it exhibits an adaptive and derived mode of development - paedomorphosis - that has evolved rapidly and independently among tiger salamanders. Unlike related tiger salamanders that undergo metamorphosis, axolotls retain larval morphological traits into adulthood and thus present an adult body plan that differs dramatically from the ancestral (metamorphic) form. The basis of paedomorphic development was investigated by comparing temporal patterns of gene transcription between axolotl and tiger salamander larvae (Ambystoma tigrinum tigrinum) that typically undergo a metamorphosis. RESULTS: Transcript abundances from whole brain and pituitary were estimated via microarray analysis on four different days post hatching (42, 56, 70, 84 dph) and regression modeling was used to independently identify genes that were differentially expressed as a function of time in both species. Collectively, more differentially
Cover: Image of an Ambystoma mexicanum limb that has been stained with alizarin red (bone) and alcian blue (cartilage). An ectopic limb (right side) was induced to grow from a wound on the limb proper by deviating a severed nerve and grafting tissue from the opposite limb axis into the wound site. This assay, known as the accessory limb assay, makes it possible to test each of the critical components of limb regeneration: (1) the wound, (2) neurotrophic factors and (3) positional information. Understanding the role of each of these components during limb regeneration in the adult A. mexicanum will bring us closer to harnessing the regenerative capacity in humans. Image by Catherine McCusker from the Gardiner/Bryant research group. See article by McCusker and Gardiner on page 593. ...
Amputation of the axolotl forelimb results in the formation of a blastema, a transient tissue where progenitor cells accumulate prior to limb regeneration. However, the molecular understanding of blastema formation had previously been hampered by the inability to identify and isolate blastema precur …
The adult human body is composed of many different types of cells arranged according to specific instructions to form tissues and organs that interconnect harmoniously and function as a complete biological unit. This is a result of events that occur during embryogenesis, which coordinate the patterning and differentiation of cells and gradually build up the complexity of the structures throughout our bodies until we reach adulthood. This developmental process is also recapitulated during regeneration in organisms that can regenerate complicated body structures, such as limbs. However, one key difference is that the cells of the regenerating system arise from and are connected to adult tissues that have already completed patterning and differentiation. Thus, regeneration requires not only the development of new pattern and the differentiation of cells to fit into this pattern, but also for the new pattern to align perfectly with the existing pattern, and for the new cells to differentiate and ...
Alone among vertebrates, urodele amphibians are able to regenerate lost body parts as adults. The key to this ability is that limb cells are triggered to dedifferentiate and reinitiate growth and pattern formation. Our strategy is to use axolotls (Ambystoma mexicanum) to discover the signals that trigger the regeneration response, in the belief that these signals have enormous potential and consequences for human health. Our long term goals are to identify the regeneration-enabling signals in limbs, in order to support progress towards the eventual application of these molecules to the improvement of human repair mechanisms. In current research, we are using assays derived from the extensive experimental history of regenerating limbs, to test the roles of several signaling molecules known to be essential for limb development. These assays are designed to examine the signals that initiate regeneration, those that are needed to establish a blastema, as well as those that are required for ...
Neoteny has been observed in many other species. It is important to note the difference between partial and full neoteny when looking at other species in order to distinguish between juvenile traits that are only advantageous in the short term and traits that provide a benefit throughout the organisms life; this might then provide some insight into the cause of neoteny in those species. Partial neoteny is the retention of the larval form beyond the usual age of maturation with the possibility of the development of sexual organs progenesis, but eventually the organism still matures into the adult form; this can be seen in Lithobates clamitans. Full neoteny is seen in Ambystoma mexicanum and some populations of Ambystoma tigrinum, which remain in their larval form for the duration of their life.[30][31] Lithobates clamitans is partially neotenous: it delays its maturation through the winter season, because it is not advantageous for it to metamorphose into the adult form until there are more ...
Neoteny (/niːˈɒtɪni/ /niːˈɒtni/ or /niːˈɒtəni/, (also called juvenilization) is the delaying or slowing of the physiological (or somatic) development of an organism, typically an animal. Neoteny is found in modern humans. In progenesis (also called paedogenesis), sexual development is accelerated. Both neoteny and progenesis result in paedomorphism (or paedomorphosis), a type of heterochrony. Some authors define paedomorphism as the retention of larval traits, as seen in salamanders. Both neoteny and progenesis cause the retention in adults of traits previously seen only in the young. Such retention is important in evolutionary biology, domestication, and evolutionary developmental biology. The origins of the concept of neoteny have been traced to the Bible (as argued by Ashley Montagu) and to the poet William Wordsworths "The Child is the father of the Man" (as argued by Barry Bogin). The term itself was invented in 1885 by Julius Kollmann as he described the axolotls maturation ...
The combined map distance of our 14 largest linkage groups (5251 cM) is consistent with previous studies that indicated a large genetic map for Ambystoma. By comparison, the combined map length of LG1 and LG2 is greater than the total map length of the mouse genome! Marker-based estimates of genome size vary greatly for Ambystoma (e.g., 2600-6276 cM in Armstrong 1984 and 7291 cM in Voss et al. 2001). This variation may be attributed to the large genome size of Ambystoma and the nonrobust nature of estimators; genotyping errors and missing data cause upward bias in size estimates and nonrandom distribution of markers with respect to recombinational distances causes downward bias (Chakravarti et al. 1991). We estimated genome size using linkage data from this study and the method of Hulbert et al. (1988). Analyses were performed separately for protein coding and anonymous marker classes and for the combined data set using several linkage thresholds (10, 15, 20, 25, and 30 cM; data not shown). ...
Im trying to locate any axolotl cDNA or genomic libraries (preferably Ambystoma mexicanum) and I would be very grateful for any help from members of the urodeles newsgroup. For the cDNA libraries I am particularly interested in embryonic stages (up to tailbud) and in adult skeletal or cardiac muscle. Yours sincerely, Tim Mohun ****************************************************** T. J. Mohun National Institute for Medical Research, Mill Hill, London NW7 1AA, UK Tel: 0181-959 3666 x2522 Fax: 0181-906 4477 Internet: tmohun at nimr.mrc.ac.uk ...
Were saddened to learn that the alien-looking Axolotl salamander (Ambystoma mexicanum), aka Mexican walking fish or Mexican water monster, is seriously
The exchange of isotopic water, 2H2O and H2(18)O has been studied in amphibian eggs. The experiments were carried out with ovarian and body cavity eggs of Rana temporaria and unfertilized eggs of Ambystoma mexicanum. The cytoplasmic diffusion coefficient for H2(18)O was found to be 4.6 × 10(−6) cm2/s, somewhat higher than that for 2H2O, 3.4 × 10(−6) cm2/s. The total change in reduced weight, delta RW, during the isotope experiments was compared with the total amount of water in the egg cell, m. The ratio delta RW/m was significantly higher than would be expected from calculations using ordinary water density values. The results are discussed in terms of different phases of structured cell water.. ...
Following amputation, urodele salamander limbs reprogram somatic cells to form a blastema that self-organizes into the missing limb parts to restore the structure and function of the limb. To help understand the molecular basis of blastema formation, we used quantitative label-free liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS)-based methods to analyze changes in the proteome that occurred 1, 4 and 7 days post amputation (dpa) through the mid-tibia/fibula of axolotl hind limbs. We identified 309 unique proteins with significant fold change relative to controls (0 dpa), representing 10 biological process categories: (1) signaling, (2) Ca2+ binding and translocation, (3) transcription, (4) translation, (5) cytoskeleton, (6) extracellular matrix (ECM), (7) metabolism, (8) cell protection, (9) degradation, and (10) cell cycle. In all, 43 proteins exhibited exceptionally high fold changes. Of these, the ecotropic viral integrative factor 5 (EVI5), a cell cycle-related oncoprotein that
The objective of this experiment is to determine whether or not axolotls can acquire spatial learning through the use of visual cues. Since salamanders, a very similar species to axolotls, have shown the ability to use landmarks as visual cues when finding food, axolotls should also be able to do so, since they are very closely related. The axolotls will be given a certain amount of time to find an escape hole, based on standardized visual cues. and with each trial, the time will be recorded. It is hypothesized that the amount of time required for the axolotl to escape the shallow water will change based on the placement of the visual cues and the amount of trials done. The independent variable will be the placement of the standardized cues, and the dependent variable will be the time it takes for the axolotl to escape. The amount of time for the experiment and data collection will be about 2 weeks. In those two weeks, using four axolotls, 140 trials will be conducted with each axolotl. Data ...
Salsite, ambystoma, axolotls in research, regeneration, limb regeneration, brain, metamorphosis, paedomorphosis, andersoni, mexicanum, tigrinum, stock center
Salsite, ambystoma, axolotls in research, regeneration, limb regeneration, brain, metamorphosis, paedomorphosis, andersoni, mexicanum, tigrinum, stock center
Salsite, ambystoma, axolotls in research, regeneration, limb regeneration, brain, metamorphosis, paedomorphosis, andersoni, mexicanum, tigrinum, stock center
I joined the Westminster Department of Biology in the fall of 2001. I teach Molecular Genetics & Heredity and Recombinant DNA as well as a number of introductory courses for the major and the Westminster liberal arts curriculum. My research interests focus on inhibition of microbial pathogens using oligonucleotides, and expression of Hox genes during salamander limb regeneration. Areas of teaching interest include scientifc literacy and enhanced dissemination and understanding of the Human Genome Project (HGP). I am also co-coordinator of the molecular biology major and currently serve on the Medical Professions Advisory Committee (MedPAC ...
A. mexicanums life cycle is rather special, since this species exhibits neoteny, which means that it retains its larval form into adulthood and becomes...
axolotl precioso, originally uploaded by girl next door ... In the canals beneath Mexico City and in the lake that surrounds it lives the Axolotl or Water Monster. It is an ancient race of up to 2 ft long salamander that Aztecs say are the direct descendents of Xototl, the dog headed god of Death.…
axolotl precioso, originally uploaded by girl next door ... In the canals beneath Mexico City and in the lake that surrounds it lives the Axolotl or Water Monster. It is an ancient race of up to 2 ft long salamander that Aztecs say are the direct descendents of Xototl, the dog headed god of Death.…
Teplits N.A., 1974: Labeled amino acid incorporation into proteins of muscle cell organelles during restoration of the x ray suppressed regenerative ability of axolotl limb
The axolotl (pronounced AHK-so-LA-tuhl) or walking fish is a rare Mexican salamander that forever looks like a teenager. It looks forever young.
Native of Mexico, of the two lakes Xochimilco and Chalco, the Axolotl is a kind of Salamander which seems to remain all its life in larval mode even after it
Hi all, My name is Hayley, Im 21 from Northamptonshire, England. I have recently purchased my first Axolotl, a three month old albino called Oscar.
Free Online Library: Axolotl Appoints Robert Scarbrough as Director of Information Technology Operations. by Business Wire; Business, international Computer services industry Executives Appointments, resignations and dismissals Information technology services industry Vice presidents (Organizations)
My 5 year old leucistic female axolotl has become slow, stiff and not at all interested in eating. I noticed her change in behaviour because shes
The paper: Murthy, A. et al. (2014) A Crohns disease variant in Atg16l1 enhances its degradation by caspase 3. Nature 506:546-562. doi:10.1038/nature13044 Subject.... ...
Buy the Paperback Book The Development of the Larval Pigment Patterns in Triturus alpestris and Ambystoma mexicanum by Hans-Henning Epperlein at Indigo.ca, Canadas largest bookstore. + Get Free Shipping on books over $25!
The ability to form a regeneration blastema, which leads to the epimorphic regeneration of complex body structures, is restricted to some amphibians and fish among vertebrates (Poss et al., 2003). A conundrum of regenerative biology is why mammals, with a few exceptions, do not form a blastema or a blastema-like structure despite the fact that they can functionally repair some tissues, such as skeletal muscle (Charge and Rudnicki, 2004) and liver (Fausto and Campbell, 2003). Of particular interest is whether the generation of progenitor cells during epimorphic regeneration in salamander and during mammalian tissue repair proceeds by the activation of different or overlapping mechanisms. A unique feature of blastema formation in salamanders is the process of dedifferentiation of stump tissues that follows appendage removal. The possibility to induce blastema formation and regeneration in mammals through the activation of a comparable dedifferentiation program has been proposed (Hughes, 2001; ...
Salamandra talpoidea Holbrook, 1838, N. Am. Herpetol., 3: 117, pl. 29. Type(s): Specimen figured on pl. 29 of the original; not known to still exist. Type locality: sea islands on the borders of South Carolina, USA.. Ambystoma ? talpoideum - Gray, 1850, Cat. Spec. Amph. Coll. Brit. Mus., Batr. Grad.: 36.. Amblystoma talpoideum - Cope, 1868 1867, Proc. Acad. Nat. Sci. Philadelphia, 19: 172.. Ambystoma talpoideum - Hay, 1892, Annu. Rep. Dept. Geol. Nat. Res. Indiana for 1891: 582.. Ambystoma (Ambystoma) talpoideum - Tihen, 1958, Bull. Florida State Mus., Biol. Sci., 3: 3, 38; Dubois and Raffaëlli, 2012, Alytes, 28: 77-161. See comment under Ambystoma regarding subgenera. ...
In order to delineate further the molecular evolution of the renin-angiotensin system in vertebrates, angiotensin I (ANG I) has been isolated after incubation of plasma and kidney extracts of emu (Dromiceus novaehollandiae), axolotl (Ambystoma mexicanum), and sea lamprey (Petromyzon marinus). The identified sequences were [Asp1, Val5, Asn9] ANG I in emu, [Asp1, Val5, His9] ANG I in axolotl, and [Asn1, Val5, Thr9] ANG I in sea lamprey. These results confirmed the previous findings that tetrapods have Asp and fishes including cyclostomes have Asn at the N-terminus, and that the amino acid residue at position 9 of ANG I was highly variable but, those at other positions were well conserved among different species. Since Asp and Asn are convertible during incubation, angiotensinogen sequences were searched in the genome and/or EST database to determine the N-terminal amino acid residue from the gene. The screening detected 12 tetrapod (10 mammalian, one avian, and one amphibian) and seven teleostean ...
An assay that detects position-related differences in affinity of axolotl regeneration blastema cells in vivo was used to test whether retinoic acid, which proximalizes regenerate pattern, simultaneously proximalizes blastema cell affinity. The assay involved autografting or homografting late bud forelimb blastomas derived from the wrist, elbow or midupper arm levels to the dorsal surface of the blastema-stump junction of an ipsilateral, medium-bud-stage hindlimb regenerating from the midthigh level. The grafted blastemas consistently displaced to their corresponding levels on the proximodistal axis of the host regenerate, indicating the existence of level-specific differences in blastema cell affinity. Retinoic acid proximalized the pattern of donor forelimb regenerates to the level of the girdle and abolished their displacement behaviour on untreated host hindlimbs. Conversely, untreated forelimb donor blastemas displaced distally to their corresponding levels on host ankle regenerates, that ...
Aryl hydrocarbon hydroxylase (AHH) was induced 15-fold in Ambystoma tigrinum by intraperitoneal injection of 3-methylcholanthrene in corn oil, or 10-fold by addition of aromatic polycyclic hydrocarbons to the aqueous environment of the neotene animal. The cytochrome P-450-associated microsomal enzyme is similar to the inducible, one-gene, autosomal-dominant system typical in the laboratory mouse and man. Differences in optimal temperature for enzyme induction and activity were noted in organ culture of human and Ambystoma tissues, and ratios of benzpyrene metabolites differed between Ambystoma and Mus. The half life of enzyme activity induced in vivo was related to the excretion of hydrocarbon metabolites.
The axolotl is a popular exotic pet like its relative, the tiger salamander (Ambystoma tigerinum). As for all poikilothermic organisms, lower temperatures result in slower metabolism and a very unhealthily reduced appetite. Temperatures at approximately 16 °C (61 °F) to 18 °C (64 °F) are suggested for captive axolotls to ensure sufficient food intake; stress resulting from more than a days exposure to lower temperatures may quickly lead to disease and death, and temperatures higher than 24 °C (75 °F) may lead to metabolic rate increase, also causing stress and eventually death.[26][27] Chlorine, commonly added to tapwater, is harmful to axolotls. A single axolotl typically requires a 40-litre (11-US-gallon) tank with a water depth of at least 15 cm (6 in). Axolotls spend the majority of the time at the bottom of the tank.[citation needed]. Salts, such as Holtfreters solution, are usually added to the water to prevent infection.[28]. In captivity, axolotls eat a variety of readily ...
As salamanders (member of the amphibian order Caudata), axolotls lack scales and claws, have the presence of a tail in all larvae, juveniles, and adults, and have forelimbs and hindlimbs that are typically about the same size and set at right angles to the body (Larson et al. 2006). They have the characteristically slender bodies, short legs, long tails, and moist, smooth skin of salamanders. Salamanders generally have a biphasic life cycle, typified by an aquatic larval stage with external gills and a terrestrial adult form that utilizes lungs or breathes through moist skin (Larson et al. 2006). However, axolotls typically remain aquatic throughout life, not undergoing metamorphosis to a terrestrial adult but retaining their juvenile features. They can undergo transformation to the adult form under certain experimental and environmental conditions. Axolotls are members of the Ambystoma tigrinum (Tiger salamander)-complex, along with all other Mexican species of Ambystoma. Ambystoma, the mole ...
Stochastic specification of primordial germ cells from mesoderm precursors in axolotl embryos[7] "A common feature of development in most vertebrate models is the early segregation of the germ line from the soma. For example, in Xenopus and zebrafish embryos primordial germ cells (PGCs) are specified by germ plasm that is inherited from the egg; in mice, Blimp1 expression in the epiblast mediates the commitment of cells to the germ line. How these disparate mechanisms of PGC specification evolved is unknown. Here, in order to identify the ancestral mechanism of PGC specification in vertebrates, we studied PGC specification in embryos from the axolotl (Mexican salamander), a model for the tetrapod ancestor. In the axolotl, PGCs develop within mesoderm, and classic studies have reported their induction from primitive ectoderm (animal cap). We used an axolotl animal cap system to demonstrate that signalling through FGF and BMP4 induces PGCs. The role of FGF was then confirmed in vivo. We also ...
Addresses: Olsson L, UPPSALA UNIV, DEPT ENVIRONM & DEV BIOL, NORBYVAGEN 18A, S-75236 UPPSALA, SWEDEN. REFERENCE CTR ONCOL, EXPTL DIV 2, I-33084 AVIANO, ITALY. CASE WESTERN RESERVE UNIV, DEPT BIOL, CLEVELAND, OH 44106.Available from: 2008-10-17 Created: 2008-10-17 Last updated: 2011-01-15 ...
Limb regeneration is observed in certain members of the animal phyla. Some animals keep this ability during their entire life while others lose it at some time during development. How do animals regenerate limbs? Is it possible to find unifying, conserved mechanisms of limb regeneration or have diff …
Digital images of male genitalia. A-CCoarazuphium whiteheadi, new species D-FZuphioides mexicanum (Chaudoir). A, D left lateral aspect B, E dorsal aspect C,
Before the zebrafishs fins regenerate, the wound is closed with multiple layers of tissue. The cells beneath the stump then lose their identity and form what is called blastema.. Researchers found that the fish uses a special genetic trick that allows the retinoic acid to control the formation of blastema, which means the animal is able to produce a store of cells that can rebuild the fin. ...
Remember when Mom said she had eyes in the back of her head? She might not be speaking metaphorically. Scientists at Tufts University have found a way to develop cells into eyes. In a discovery reminiscent of Frankenstein (the movie, … Continue reading →. ...
listen); plural axolotls or rarely axolomeh[3]), Ambystoma mexicanum,[2] also known as the Mexican walking fish, is a neotenic ... Frost, Darrel R. (2018). "Ambystoma mexicanum (Shaw and Nodder, 1798)". Amphibian Species of the World: an Online Reference. ... "Mexican Walking Fish, Axolotls Ambystoma mexicanum" (PDF). Archived from the original (PDF) on 15 Mar 2018.. ... "Caudata Culture Species Entry - Ambystoma mexicanum - Axolotl". www.caudata.org. Retrieved 2016-03-14.. ...
Ambystoma mexicanum) 28 [49] Bed bug. (Cimex lectularius) 29-47 26 autosomes and varying number of the sex chromosomes from ... "Initial characterization of the large genome of the salamander Ambystoma mexicanum using shotgun and laser capture chromosome ...
Progenesis (or paedogenesis) can be observed in the axolotl (Ambystoma mexicanum). Axolotls reach full sexual maturity while ... For example, some individuals of the salamander species Ambystoma talpoideum delay the metamorphosis of the skull. Reilley et ... CS1 maint: Uses authors parameter (link) Semlitsch, Raymond D. (1987). "Paedomorphosis in Ambystoma talpoideum: effects of ... Reilly, Stephen M. (February 1987). "Ontogeny of the hyobranchial apparatus in the salamanders Ambystoma talpoideum ( ...
Ambystoma mexicanum, an aquatic salamander, is a species protected under the Mexican UMA (Unit for Management and conservation ... Figiel, Chester (2013). "Cryopreservation of Sperm from the Axolotl AmbystomA MexicAnum: ImpliCations for Conservation" (PDF). ... Ambystoma mexicanum), the tissues are seemingly unresponsive to the hormones. In other species, the changes may not be ... and Ambystoma mexicanum (32 Gb) have been sequenced. Legends have developed around the salamander over the centuries, many ...
"Axolotl, Ajolote Ambystoma mexicanum" (in Spanish). Xochimilco, Mexico City: Borough of Xochimilco. Retrieved February 25, 2011 ... Ambystoma mexicanum). This amphibian was used as a medicine, food and ceremonial object during the Aztec empire. It was ...
He is remembered for his research involving limb regeneration and parabiosis of the Mexican axolotl (Ambystoma mexicanum). He ... Julius Schaxel and the end of the Evo-Devo agenda in Jena Ambystoma.org at the Wayback Machine (archived December 5, 2008) ...
Ambystoma mexicanum) as a model genetic organism. This progress has been facilitated by advances in genomics, bioinformatics, ... Ambystoma mexicanum)". Dev Biol. 366: 374-381. doi:10.1016/j.ydbio.2012.03.022. PMID 22537500. Christensen, RN; Tassava, RA ( ... The Ambystoma Genetic Stock Center (AGSC) is a self-sustaining, breeding colony of the axolotl supported by the National ... An NIH-funded NCRR grant has led to the establishment of the Ambystoma EST database, the Salamander Genome Project (SGP) that ...
Amandava formosa Amblyrhynchus cristatus Ambystoma dumerilii Ambystoma mexicanum Ameerega spp. Ammotragus lervia Amyda ...
The axolotl (Ambystoma mexicanum) retains its larval form with gills into adulthood ... Shaffer, H. Bradley (2005). "Ambystoma gracile". AmphibiaWeb. Retrieved November 21, 2012.. *^ Kiyonaga, Robin R. " ... Some members of the genera Ambystoma and Dicamptodon have larvae that never fully develop into the adult form, but this varies ... The tiger salamander (Ambystoma tigrinum) is typical of the frogs and salamanders that hide under cover ready to ambush unwary ...
The axolotl (Ambystoma mexicanum) retains its larval form with gills into adulthood ...
It should not be confused with the axolotl, a salamander (Ambystoma mexicanum), which is usually called ajolote in Spanish. It ...
For studies of regeneration urodele amphibians such as the axolotl Ambystoma mexicanum are used, and also planarian worms such ...
Ambystoma mexicanum). The contraction wave's trajectory was more complex than predicted in the original model however it did ... ectoderm cells differentiating to neural plate and epidermis during gastrulation in embryos of the axolotl Ambystoma mexicanum ...
Ambystoma mexicanum)". Developmental Biology. 366 (2): 374-81. doi:10.1016/j.ydbio.2012.03.022. PMID 22537500.. ... Ambystoma mexicanum) as a model genetic organism. This progress has been facilitated by advances in genomics, bioinformatics, ... "Ambystoma.org.. *^ a b Liversage RA, Anderson M, Korneluk RG (February 2005). "Regenerative response of amputated forelimbs of ... An NIH-funded NCRR grant has led to the establishment of the Ambystoma EST database, the Salamander Genome Project (SGP) that ...
Full neoteny is seen in Ambystoma mexicanum and some populations of Ambystoma tigrinum, which remain in their larval form for ... Ambystoma tigrinum retains its neotenous features for a similar reason, however the retention is permanent due to the lack of ... Snyder, R (1956). "Comparative Features of the Life Histories of Ambystoma gracile (Baird) from Populations at Low and High ... doi:10.1016/0020-7322(87)90022-5. Eagleson, G.; McKeown, B. (1978). "Changes in thyroid activity of Ambystoma gracile (Baird) ...
Ambystoma mexicanum). The principal natural resource, aside from minerals, are the forests, being present on 68% of the ...
Ambystoma mexicanum) African clawed frog (Xenopus laevis) Indian python (Python molurus) Rock monitor (Varanus albigularis) ...
... ambystoma MeSH B01.150.900.090.608.080.068.525 --- ambystoma mexicanum MeSH B01.150.900.090.608.630 --- proteidae MeSH B01.150. ... leishmania mexicana MeSH B01.500.841.750.443.950.450.868.488.680 --- leishmania tropica MeSH B01.500.841.750.443.950.450.868. ...
Ambystoma mexicanum, axolotl (2018) Xenopus tropicalis, western clawed frog (2010) Nanorana parkeri, High Himalaya frog (2015) ...
... such as Ambystoma mexicanum Amphibious fish Aquatic animal Aquatic ecology Aquatic mammal Diving bird Freshwater biology Marine ...
2010) have written that the Mexican axolotl (Ambystoma mexicanum) could be classified as a hopeful monster as it exhibits an ...
Ambystoma mexicanum) Leucistic red-tailed hawk (Buteo jamaicensis) Leucistic western jackdaw (Corvus monedula) in Naantali, ...
Ambystoma mexicanum (diff). *Monarquía absoluta (diff). *EX-118 (diff). *Partido Socialista (Argentina) (diff) ...
Ambystoma granulosum (CR) Ambystoma leorae (CR) Ambystoma lermaense (CR) Ambystoma mexicanum (CR) Ambystoma ordinarium (EN) ... Ambystoma rosaceum (LC) Ambystoma silvense (DD) Ambystoma taylori (CR) Ambystoma tigrinum (LC) Ambystoma velasci (LC) Order: ... Ambystoma amblycephalum (CR) Ambystoma andersoni (CR) Ambystoma bombypellum (CR) Ambystoma dumerilii (CR) Ambystoma ... Family: Dermophiidae Dermophis mexicanus (VU) Dermophis oaxacae (DD) Order: Caudata. Family: Ambystomatidae Ambystoma ...
Full neoteny is seen in Ambystoma mexicanum and some populations of Ambystoma tigrinum, which remain in their larval form for ... Ambystoma tigrinum retains its neotenous features for a similar reason, however the retention is permanent due to the lack of ... Snyder, R (1956). "Comparative Features of the Life Histories of Ambystoma gracile (Baird) from Populations at Low and High ... In other species, environmental conditions cause neoteny, as in the northwestern salamander (Ambystoma gracile), where higher ...
Gymnopis syntrema · Dermophis gracilior · Dermophis mexicanus · Dermophis costaricensis · Dermophis glandulosus · Dermophis ... Axolotls, from the genus Ambystoma (or mole salamanders), are neotenic amphibians. This means they get to sexual maturity and ...
Buy the Paperback Book The Development of the Larval Pigment Patterns in Triturus alpestris and Ambystoma mexicanum by Hans- ... The Development of the Larval Pigment Patterns in Triturus alpestris and Ambystoma mexicanum. byHans-Henning Epperlein, Jan ... Title:The Development of the Larval Pigment Patterns in Triturus alpestris and Ambystoma mexicanumFormat:PaperbackDimensions: ... Customer Reviews of The Development of the Larval Pigment Patterns in Triturus alpestris and Ambystoma mexicanum. ...
Glycosaminoglycans compositional analysis of Urodele axolotl (Ambystoma mexicanum) and Porcine Retina. *So Young Kim1. , ... Kim, S.Y., Kundu, J., Williams, A. et al. Glycosaminoglycans compositional analysis of Urodele axolotl (Ambystoma mexicanum) ... Voss, S.R., Epperlein, H.H., Tanaka, E.M.: Ambystoma mexicanum, the axolotl: a versatile amphibian model for regeneration, ... Humans cannot regenerate retina, however, axolotl (Ambystoma mexicanum), a laboratory-bred salamander, can regenerate retinal ...
Ambystoma mexicanum) is a critically endangered salamander species and a model organism for regenerative and developmental ... Page, R. B. & Voss, S. R. Induction of metamorphosis in axolotls (Ambystoma mexicanum). Cold Spring Harb Protoc 2009, ... Axolotl (Ambystoma mexicanum) is a critically endangered salamander species and a model organism for regenerative and ... Axolotl (Ambystoma mexicanum), a salamander species of amphibians, possess experimentally validated features, such as high ...
Threads in Forum : Axolotls (Ambystoma mexicanum). Forum Tools Search this Forum. Views: 21,341 Announcement: Axolotls are ... Axolotls (Ambystoma mexicanum) A dedicated topic for those seeking help with Axolotls, showing off your photos, or just to talk ... Caudata.org , Newt & Salamander Forum , Beginner Newt, Salamander, Axolotl & Help Topics , Axolotls (Ambystoma mexicanum) ...
The Mexican axolotl, Ambystoma mexicanum, is a strictly aquatic species that persists currently in two highly threatened and ... The Case of Ambystoma mexicanum (Caudata, Ambystomatidae)," Annales Zoologici Fennici, 47(4), 223-238, (1 August 2010) Include: ... mexicanum samples. Our data revealed higher haplotypic diversity in A. mexicanum populations than previously recorded, due to ... The Case of Ambystoma mexicanum (Caudata, Ambystomatidae)," Annales Zoologici Fennici 47(4), 223-238, (1 August 2010). https:// ...
This publication sets the stage for the use of the axolotl (Ambystoma mexicanum) as an excellent model to study regeneration ... Rôle des Smads lors du processus de régénération chez Ambystoma mexicanum. Thesis or Dissertation ... Aussi, cette publication positionne laxolotl (Ambystoma mexicanum) comme un excellent modèle pour étudier le processus de ...
In spite of numerous investigations of regenerating salamander limbs, little attention has been paid to the details of how joints are reformed. An understanding of the process and mechanisms of joint regeneration in this model system for tetrapod limb regeneration would provide insights into developing novel therapies for inducing joint regeneration in humans. To this end, we have used the axolotl (Mexican Salamander) model of limb regeneration to describe the morphology and the expression patterns of marker genes during joint regeneration in response to limb amputation. These data are consistent with the hypothesis that the mechanisms of joint formation whether it be development or regeneration are conserved. We also have determined that defects in the epiphyseal region of both forelimbs and hind limbs in the axolotl are regenerated only when the defect is small. As is the case with defects in the diaphysis, there is a critical size above which the endogenous regenerative response is not sufficient to
Experimental studies on a mutant gene (p) causing premature death of Ambystoma mexicanum embryos ... Experimental studies on a mutant gene (p) causing premature death of Ambystoma mexicanum embryos ... Experimental studies on a mutant gene (p) causing premature death of Ambystoma mexicanum embryos ... Experimental studies on a mutant gene (p) causing premature death of Ambystoma mexicanum embryos ...
Re: Ambystoma mexicanum breeding report---leucistic axolotl. by Phran » Wed Jul 10, 2013 5:41 pm ... Re: Ambystoma mexicanum breeding report---leucistic axolotl. by Lillypod » Wed Jul 10, 2013 5:52 pm ... Re: Ambystoma mexicanum breeding report---leucistic axolotl. by cascade » Thu Jul 11, 2013 9:04 pm ... Re: Ambystoma mexicanum breeding report---leucistic axolotl. by wadekilian » Thu Jul 11, 2013 9:38 pm ...
The clear differences in the concentration between enkephalins through a submammalian brain species as Ambystoma genus and the ... diencephalon and hypophysis of Ambystoma mexicanum brain by radioimmunoassay procedure. The met-enkephalin was the predominant ... Ambystoma mexicanum).. @article{Asai1988IRMetAI, title={IR-Met and IR-Leu enkephalin content in the axolotl brain (Ambystoma ... diencephalon and hypophysis of Ambystoma mexicanum brain by radioimmunoassay procedure. The met-enkephalin was the predominant ...
Subject: Re: Ambystoma mexicanum (the ) Wed Mar 06, 2013 11:35 am. ... Subject: Re: Ambystoma mexicanum (the ) Thu Mar 21, 2013 7:36 pm. ...
Ambystoma mexicanum, commonly known as the axolotl, possesses extraordinary regenerative abilities and is capable of ... The Ambystoma mexicanum has a large genome composed of repetitive DNA components and functional elements (Smith et al., 2009). ... The Ambystoma mexicanum, commonly known as the axolotl, possesses extraordinary regenerative abilities and is capable of ... 2004). An Ambystoma mexicanum EST sequencing project: analysis of 17,352 expressed sequence tags from embryonic and ...
Axolotl - Ambystoma mexicanum. Victoria Heuer. Oct 28, 2017. Russian Tortoise - Agrionemys horsfieldii. ...
Application and optimization of CRISPR-Cas9-mediated genome engineering in axolotl (Ambystoma mexicanum) *Ji-Feng Fei ... Publisher Correction: Application and optimization of CRISPR-Cas9-mediated genome engineering in axolotl (Ambystoma mexicanum) ... Ambystoma mexicanum,/i,) . Opens in a new window. ... Ambystoma mexicanum,/i,) . Opens in a new window. ...
listen); plural axolotls or rarely axolomeh[3]), Ambystoma mexicanum,[2] also known as the Mexican walking fish, is a neotenic ... Frost, Darrel R. (2018). "Ambystoma mexicanum (Shaw and Nodder, 1798)". Amphibian Species of the World: an Online Reference. ... "Mexican Walking Fish, Axolotls Ambystoma mexicanum" (PDF). Archived from the original (PDF) on 15 Mar 2018.. ... "Caudata Culture Species Entry - Ambystoma mexicanum - Axolotl". www.caudata.org. Retrieved 2016-03-14.. ...
Ambystoma mexicanum) 28 [49] Bed bug. (Cimex lectularius) 29-47 26 autosomes and varying number of the sex chromosomes from ... "Initial characterization of the large genome of the salamander Ambystoma mexicanum using shotgun and laser capture chromosome ...
Latina: Ambystoma mexicanum. English: axolotl. Deutsch: Axolotl. Reptilians / Reptilien[edit]. English: species ...
Axolotls (Ambystoma mexicanum) A dedicated topic for those seeking help with Axolotls, showing off your photos, or just to talk ...
Ambystoma Mexicanum), Real-time Digital Imaging of Leukocyte-endothelial Interaction in Ischemia-reperfusion Injury (IRI) of ... 2D and 3D Echocardiography in the Axolotl (Ambystoma Mexicanum). Anita Dittrich1, Mathias Møller Thygesen1, Henrik Lauridsen1 ...
Ajolote (Ambystoma mexicanum).jpg 960 × 719; 388 KB. *. Alejando Piscitelli en el Tec de Monterrey 1.jpg 6,016 × 4,000; 2.82 MB ...
Comparative pelvic development of the axolotl (Ambystoma mexicanum) and the Australian lungfish (Neoceratodus forsteri): ... Comparative pelvic development of the axolotl (Ambystoma mexicanum) and the Australian lungfish (Neoceratodus forsteri): ... growth series of the Australian lungfish Neoceratodus forsteri and the Mexican axolotl Ambystoma mexicanum were cleared and ... Development of the pelvic endoskeleton and musculature is very similar in Neoceratodus and Ambystoma. If the acetabulum is seen ...
Ambystoma Mexicanum), Experimental Methods to Study Human Postural Control, Transthoracic Echocardiographic Examination in ... 2D and 3D Echocardiography in the Axolotl (Ambystoma Mexicanum). Anita Dittrich1, Mathias Møller Thygesen1, Henrik Lauridsen1 ...
Axolotl (Ambystoma mexicanum) - CR. Salamander, Chinese giant (Andrias davidianus) - CR. Reptiles. Iguana, Fiji Island banded ( ...
Ambystoma mexicanum. Axolotl Dicamptodon tenebrosus. Coastal giant salamander Lissotriton vulgaris. Smooth newt ...
The axolotl (Ambystoma mexicanum) is capable of regenerating complex biological structures throughout adulthood. One of the ... Here we have established highly efficient gene knockin approaches in the axolotl (Ambystoma mexicanum) based on the CRISPR/Cas9 ... 1975) Normal stages of development of the axolotl, Ambystoma mexicanum. Dev Biol 42:391-400. ... In this study, we have established efficient CRISPR/Cas9 mediated gene knockin approaches in the axolotl (Ambystoma mexicanum ...
  • Phylogenetic and haplotype network analyses of mitochondrial DNA sequences confirmed low genetic differentiation and a recurrent lack of monophyly in many of the taxa belonging to the Ambystoma tigrinum species group, including A. mexicanum , but clustered the Chapultepec samples with other A. mexicanum samples. (bioone.org)
  • THE tiger salamander ( Ambystoma tigrinum ) species complex consists of several closely related and phenotypically diverse taxa that range from central Mexico to southern Canada ( S haffer and M c K night 1996 ). (genetics.org)
  • estimated map size from a linkage analysis in which 347 molecular genotypes were obtained for offspring from backcrosses between A. mexicanum and A. mexicanum/A. t. tigrinum hybrids. (genetics.org)
  • To demonstrate the utility of this resource, we mapped the position of two historically significant A. mexicanum mutants, white and melanoid , and also met , a quantitative trait locus (QTL) that contributes to variation in metamorphic timing. (genetics.org)
  • Using a more direct method, C allan (1966) reported chiasmata counts from A. mexicanum oocyte nuclei ranging from 101 to 126, averaging 113 chiasmata per nucleus. (genetics.org)
  • The experiments were carried out with ovarian and body cavity eggs of Rana temporaria and unfertilized eggs of Ambystoma mexicanum. (biologists.org)
  • The peptides met- and leu-enkephalin were identified in the telencephalon, rombencephalon, diencephalon and hypophysis of Ambystoma mexicanum brain by radioimmunoassay procedure. (semanticscholar.org)
  • Herein, we describe various clinical test results, clinical outcomes, and the pathological findings of an experimental pneumonectomy procedure in 3 A. mexicanum exhibiting abnormal buoyancy. (readbyqxmd.com)
  • Homologous muscles develop in the same order with the hypaxial musculature developing first, followed by the deep, then the superficial pelvic musculature.Conclusions: Development of the pelvic endoskeleton and musculature is very similar in Neoceratodus and Ambystoma. (edu.au)
  • Aussi, cette publication positionne l'axolotl (Ambystoma mexicanum) comme un excellent modèle pour étudier le processus de régénération épimorphique ainsi que l'importance de la signalisation TGF-β. (umontreal.ca)
  • Animals in this colony are purchased from the Ambystoma Genetic Center Stock (Lexington, KY, USA) and measure between 2.0 cm to 8.5 cm from the snout to tip of the tail, at arrival. (hindawi.com)
  • As far as we are aware, there are no previous reports on the pathologic conditions of buoyancy disorders in Ambystoma mexicanum. (readbyqxmd.com)