Mannosidases: Glycoside hydrolases that catalyze the hydrolysis of alpha or beta linked MANNOSE.alpha-Mannosidase: An enzyme that catalyzes the HYDROLYSIS of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides. The enzyme plays a role in the processing of newly formed N-glycans and in degradation of mature GLYCOPROTEINS. There are multiple isoforms of alpha-mannosidase, each having its own specific cellular location and pH optimum. Defects in the lysosomal form of the enzyme results in a buildup of mannoside intermediate metabolites and the disease ALPHA-MANNOSIDOSIS.Swainsonine: An indolizidine alkaloid from the plant Swainsona canescens that is a potent alpha-mannosidase inhibitor. Swainsonine also exhibits antimetastatic, antiproliferative, and immunomodulatory activity.1-Deoxynojirimycin: An alpha-glucosidase inhibitor with antiviral action. Derivatives of deoxynojirimycin may have anti-HIV activity.Oligosaccharides: Carbohydrates consisting of between two (DISACCHARIDES) and ten MONOSACCHARIDES connected by either an alpha- or beta-glycosidic link. They are found throughout nature in both the free and bound form.Mannose: A hexose or fermentable monosaccharide and isomer of glucose from manna, the ash Fraxinus ornus and related plants. (From Grant & Hackh's Chemical Dictionary, 5th ed & Random House Unabridged Dictionary, 2d ed)Golgi Apparatus: A stack of flattened vesicles that functions in posttranslational processing and sorting of proteins, receiving them from the rough ENDOPLASMIC RETICULUM and directing them to secretory vesicles, LYSOSOMES, or the CELL MEMBRANE. The movement of proteins takes place by transfer vesicles that bud off from the rough endoplasmic reticulum or Golgi apparatus and fuse with the Golgi, lysosomes or cell membrane. (From Glick, Glossary of Biochemistry and Molecular Biology, 1990)PolysaccharidesAlkaloids: Organic nitrogenous bases. Many alkaloids of medical importance occur in the animal and vegetable kingdoms, and some have been synthesized. (Grant & Hackh's Chemical Dictionary, 5th ed)Mannosidase Deficiency Diseases: Diseases caused by the loss of one or more enzymes involved in the hydrolysis of mannoside linkages (MANNOSIDASES). The defects in enzyme activity are primarily associated with genetic mutation of the genes that codes for a particular mannosidase isoenzyme.alpha 1-Antitrypsin: Plasma glycoprotein member of the serpin superfamily which inhibits TRYPSIN; NEUTROPHIL ELASTASE; and other PROTEOLYTIC ENZYMES.Imino Furanoses: Five-carbon furanose sugars in which the OXYGEN is replaced by a NITROGEN atom.Endoplasmic Reticulum: A system of cisternae in the CYTOPLASM of many cells. In places the endoplasmic reticulum is continuous with the plasma membrane (CELL MEMBRANE) or outer membrane of the nuclear envelope. If the outer surfaces of the endoplasmic reticulum membranes are coated with ribosomes, the endoplasmic reticulum is said to be rough-surfaced (ENDOPLASMIC RETICULUM, ROUGH); otherwise it is said to be smooth-surfaced (ENDOPLASMIC RETICULUM, SMOOTH). (King & Stansfield, A Dictionary of Genetics, 4th ed)Carbohydrate Sequence: The sequence of carbohydrates within POLYSACCHARIDES; GLYCOPROTEINS; and GLYCOLIPIDS.IndolizinesMannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase: A group of related enzymes responsible for the endohydrolysis of the di-N-acetylchitobiosyl unit in high-mannose-content glycopeptides and GLYCOPROTEINS.Glycosylation: The chemical or biochemical addition of carbohydrate or glycosyl groups to other chemicals, especially peptides or proteins. Glycosyl transferases are used in this biochemical reaction.Glycoproteins: Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.GlucosamineMolecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Carbohydrate Conformation: The characteristic 3-dimensional shape of a carbohydrate.Receptors, Adrenergic, alpha: One of the two major pharmacological subdivisions of adrenergic receptors that were originally defined by the relative potencies of various adrenergic compounds. The alpha receptors were initially described as excitatory receptors that post-junctionally stimulate SMOOTH MUSCLE contraction. However, further analysis has revealed a more complex picture involving several alpha receptor subtypes and their involvement in feedback regulation.Pichia: Yeast-like ascomycetous fungi of the family Saccharomycetaceae, order SACCHAROMYCETALES isolated from exuded tree sap.Hypoxia-Inducible Factor 1, alpha Subunit: Hypoxia-inducible factor 1, alpha subunit is a basic helix-loop-helix transcription factor that is regulated by OXYGEN availability and is targeted for degradation by VHL TUMOR SUPPRESSOR PROTEIN.Asparagine: A non-essential amino acid that is involved in the metabolic control of cell functions in nerve and brain tissue. It is biosynthesized from ASPARTIC ACID and AMMONIA by asparagine synthetase. (From Concise Encyclopedia Biochemistry and Molecular Biology, 3rd ed)Substrate Specificity: A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.Brefeldin A: A fungal metabolite which is a macrocyclic lactone exhibiting a wide range of antibiotic activity.Kinetics: The rate dynamics in chemical or physical systems.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.alpha-Glucosidases: Enzymes that catalyze the exohydrolysis of 1,4-alpha-glucosidic linkages with release of alpha-glucose. Deficiency of alpha-1,4-glucosidase may cause GLYCOGEN STORAGE DISEASE TYPE II.Hexosaminidases: Enzymes that catalyze the hydrolysis of N-acylhexosamine residues in N-acylhexosamides. Hexosaminidases also act on GLUCOSIDES; GALACTOSIDES; and several OLIGOSACCHARIDES.Glycoside HydrolasesProtein Processing, Post-Translational: Any of various enzymatically catalyzed post-translational modifications of PEPTIDES or PROTEINS in the cell of origin. These modifications include carboxylation; HYDROXYLATION; ACETYLATION; PHOSPHORYLATION; METHYLATION; GLYCOSYLATION; ubiquitination; oxidation; proteolysis; and crosslinking and result in changes in molecular weight and electrophoretic motility.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.N-Acetylglucosaminyltransferases: Enzymes that catalyze the transfer of N-acetylglucosamine from a nucleoside diphosphate N-acetylglucosamine to an acceptor molecule which is frequently another carbohydrate. EC 2.4.1.-.Acetylglucosaminidase: A beta-N-Acetylhexosaminidase that catalyzes the hydrolysis of terminal, non-reducing 2-acetamido-2-deoxy-beta-glucose residues in chitobiose and higher analogs as well as in glycoproteins. Has been used widely in structural studies on bacterial cell walls and in the study of diseases such as MUCOLIPIDOSIS and various inflammatory disorders of muscle and connective tissue.Monensin: An antiprotozoal agent produced by Streptomyces cinnamonensis. It exerts its effect during the development of first-generation trophozoites into first-generation schizonts within the intestinal epithelial cells. It does not interfere with hosts' development of acquired immunity to the majority of coccidial species. Monensin is a sodium and proton selective ionophore and is widely used as such in biochemical studies.alpha7 Nicotinic Acetylcholine Receptor: A member of the NICOTINIC ACETYLCHOLINE RECEPTOR subfamily of the LIGAND-GATED ION CHANNEL family. It consists entirely of pentameric a7 subunits expressed in the CNS, autonomic nervous system, vascular system, lymphocytes and spleen.Immunosorbent Techniques: Techniques for removal by adsorption and subsequent elution of a specific antibody or antigen using an immunosorbent containing the homologous antigen or antibody.p-Chloromercuribenzoic Acid: An organic mercurial used as a sulfhydryl reagent.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Glucosidases: Enzymes that hydrolyze O-glucosyl-compounds. (Enzyme Nomenclature, 1992) EC 3.2.1.-.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Liver: A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.Integrin alpha3beta1: Cell surface receptor for LAMININ, epiligrin, FIBRONECTINS, entactin, and COLLAGEN. Integrin alpha3beta1 is the major integrin present in EPITHELIAL CELLS, where it plays a role in the assembly of BASEMENT MEMBRANE as well as in cell migration, and may regulate the functions of other integrins. Two alternatively spliced isoforms of the alpha subunit (INTEGRIN ALPHA3), are differentially expressed in different cell types.Integrin alpha4: An integrin alpha subunit that is unique in that it does not contain an I domain, and its proteolytic cleavage site is near the middle of the extracellular portion of the polypeptide rather than close to the membrane as in other integrin alpha subunits.Integrin alpha6: An integrin alpha subunit that primarily associates with INTEGRIN BETA1 or INTEGRIN BETA4 to form laminin-binding heterodimers. Integrin alpha6 has two alternatively spliced isoforms: integrin alpha6A and integrin alpha6B, which differ in their cytoplasmic domains and are regulated in a tissue-specific and developmental stage-specific manner.Integrin alpha5beta1: An integrin found in FIBROBLASTS; PLATELETS; MONOCYTES, and LYMPHOCYTES. Integrin alpha5beta1 is the classical receptor for FIBRONECTIN, but it also functions as a receptor for LAMININ and several other EXTRACELLULAR MATRIX PROTEINS.Cricetinae: A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.Cell Line: Established cell cultures that have the potential to propagate indefinitely.DNA, Complementary: Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.Acetylglucosamine: The N-acetyl derivative of glucosamine.Intracellular Membranes: Thin structures that encapsulate subcellular structures or ORGANELLES in EUKARYOTIC CELLS. They include a variety of membranes associated with the CELL NUCLEUS; the MITOCHONDRIA; the GOLGI APPARATUS; the ENDOPLASMIC RETICULUM; LYSOSOMES; PLASTIDS; and VACUOLES.Chromatography: Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. Usage is both analytical for small amounts and preparative for bulk amounts.Integrin alpha4beta1: Integrin alpha4beta1 is a FIBRONECTIN and VCAM-1 receptor present on LYMPHOCYTES; MONOCYTES; EOSINOPHILS; NK CELLS and thymocytes. It is involved in both cell-cell and cell- EXTRACELLULAR MATRIX adhesion and plays a role in INFLAMMATION, hematopoietic cell homing and immune function, and has been implicated in skeletal MYOGENESIS; NEURAL CREST migration and proliferation, lymphocyte maturation and morphogenesis of the PLACENTA and HEART.Coat Protein Complex I: A protein complex comprised of COATOMER PROTEIN and ADP RIBOSYLATION FACTOR 1. It is involved in transport of vesicles between the ENDOPLASMIC RETICULUM and the GOLGI APPARATUS.Interleukin-1alpha: An interleukin-1 subtype that occurs as a membrane-bound pro-protein form that is cleaved by proteases to form a secreted mature form. Unlike INTERLEUKIN-1BETA both membrane-bound and secreted forms of interleukin-1alpha are biologically active.Transfection: The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.Integrin alpha2beta1: An integrin found on fibroblasts, platelets, endothelial and epithelial cells, and lymphocytes where it functions as a receptor for COLLAGEN and LAMININ. Although originally referred to as the collagen receptor, it is one of several receptors for collagen. Ligand binding to integrin alpha2beta1 triggers a cascade of intracellular signaling, including activation of p38 MAP kinase.Galactosyltransferases: Enzymes that catalyze the transfer of galactose from a nucleoside diphosphate galactose to an acceptor molecule which is frequently another carbohydrate. EC 2.4.1.-.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Receptors, Adrenergic, alpha-1: A subclass of alpha-adrenergic receptors that mediate contraction of SMOOTH MUSCLE in a variety of tissues such as ARTERIOLES; VEINS; and the UTERUS. They are usually found on postsynaptic membranes and signal through GQ-G11 G-PROTEINS.Integrin alpha5: This integrin alpha subunit combines with INTEGRIN BETA1 to form a receptor (INTEGRIN ALPHA5BETA1) that binds FIBRONECTIN and LAMININ. It undergoes posttranslational cleavage into a heavy and a light chain that are connected by disulfide bonds.Integrin alpha1beta1: Integrin alpha1beta1 functions as a receptor for LAMININ and COLLAGEN. It is widely expressed during development, but in the adult is the predominant laminin receptor (RECEPTORS, LAMININ) in mature SMOOTH MUSCLE CELLS, where it is important for maintenance of the differentiated phenotype of these cells. Integrin alpha1beta1 is also found in LYMPHOCYTES and microvascular endothelial cells, and may play a role in angiogenesis. In SCHWANN CELLS and neural crest cells, it is involved in cell migration. Integrin alpha1beta1 is also known as VLA-1 and CD49a-CD29.Mannans: Polysaccharides consisting of mannose units.Membrane Proteins: Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.Receptors, Adrenergic, alpha-2: A subclass of alpha-adrenergic receptors found on both presynaptic and postsynaptic membranes where they signal through Gi-Go G-PROTEINS. While postsynaptic alpha-2 receptors play a traditional role in mediating the effects of ADRENERGIC AGONISTS, the subset of alpha-2 receptors found on presynaptic membranes signal the feedback inhibition of NEUROTRANSMITTER release.Sequence Homology, Amino Acid: The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.Tunicamycin: An N-acetylglycosamine containing antiviral antibiotic obtained from Streptomyces lysosuperificus. It is also active against some bacteria and fungi, because it inhibits the glucosylation of proteins. Tunicamycin is used as tool in the study of microbial biosynthetic mechanisms.Integrin alpha6beta1: A cell surface receptor mediating cell adhesion to the EXTRACELLULAR MATRIX and to other cells via binding to LAMININ. It is involved in cell migration, embryonic development, leukocyte activation and tumor cell invasiveness. Integrin alpha6beta1 is the major laminin receptor on PLATELETS; LEUKOCYTES; and many EPITHELIAL CELLS, and ligand binding may activate a number of signal transduction pathways. Alternative splicing of the cytoplasmic domain of the alpha6 subunit (INTEGRIN ALPHA6) results in the formation of A and B isoforms of the heterodimer, which are expressed in a tissue-specific manner.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Hydrolysis: The process of cleaving a chemical compound by the addition of a molecule of water.Cell Compartmentation: A partitioning within cells due to the selectively permeable membranes which enclose each of the separate parts, e.g., mitochondria, lysosomes, etc.Enzyme Inhibitors: Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.CHO Cells: CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). The species is a favorite for cytogenetic studies because of its small chromosome number. The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells.Integrin alpha6beta4: This intrgrin is a key component of HEMIDESMOSOMES and is required for their formation and maintenance in epithelial cells. Integrin alpha6beta4 is also found on thymocytes, fibroblasts, and Schwann cells, where it functions as a laminin receptor (RECEPTORS, LAMININ) and is involved in wound healing, cell migration, and tumor invasiveness.Integrin alpha Chains: The alpha subunits of integrin heterodimers (INTEGRINS), which mediate ligand specificity. There are approximately 18 different alpha chains, exhibiting great sequence diversity; several chains are also spliced into alternative isoforms. They possess a long extracellular portion (1200 amino acids) containing a MIDAS (metal ion-dependent adhesion site) motif, and seven 60-amino acid tandem repeats, the last 4 of which form EF HAND MOTIFS. The intracellular portion is short with the exception of INTEGRIN ALPHA4.Microsomes, Liver: Closed vesicles of fragmented endoplasmic reticulum created when liver cells or tissue are disrupted by homogenization. They may be smooth or rough.Integrins: A family of transmembrane glycoproteins (MEMBRANE GLYCOPROTEINS) consisting of noncovalent heterodimers. They interact with a wide variety of ligands including EXTRACELLULAR MATRIX PROTEINS; COMPLEMENT, and other cells, while their intracellular domains interact with the CYTOSKELETON. The integrins consist of at least three identified families: the cytoadhesin receptors(RECEPTORS, CYTOADHESIN), the leukocyte adhesion receptors (RECEPTORS, LEUKOCYTE ADHESION), and the VERY LATE ANTIGEN RECEPTORS. Each family contains a common beta-subunit (INTEGRIN BETA CHAINS) combined with one or more distinct alpha-subunits (INTEGRIN ALPHA CHAINS). These receptors participate in cell-matrix and cell-cell adhesion in many physiologically important processes, including embryological development; HEMOSTASIS; THROMBOSIS; WOUND HEALING; immune and nonimmune defense mechanisms; and oncogenic transformation.Integrin alpha1: An integrin alpha subunit that binds COLLAGEN and LAMININ though its I domain. It combines with INTEGRIN BETA1 to form the heterodimer INTEGRIN ALPHA1BETA1.Electrophoresis, Polyacrylamide Gel: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Cyclopentanes: A group of alicyclic hydrocarbons with the general formula R-C5H9.Mannosyltransferases: Enzymes that catalyze the transfer of mannose from a nucleoside diphosphate mannose to an acceptor molecule which is frequently another carbohydrate. The group includes EC 2.4.1.32, EC 2.4.1.48, EC 2.4.1.54, and EC 2.4.1.57.Alpha Rhythm: Brain waves characterized by a relatively high voltage or amplitude and a frequency of 8-13 Hz. They constitute the majority of waves recorded by EEG registering the activity of the parietal and occipital lobes when the individual is awake, but relaxed with the eyes closed.Protein Folding: Processes involved in the formation of TERTIARY PROTEIN STRUCTURE.Rats, Inbred Strains: Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding.Protein Structure, Tertiary: The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.Fluorescent Antibody Technique: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.Proteasome Endopeptidase Complex: A large multisubunit complex that plays an important role in the degradation of most of the cytosolic and nuclear proteins in eukaryotic cells. It contains a 700-kDa catalytic sub-complex and two 700-kDa regulatory sub-complexes. The complex digests ubiquitinated proteins and protein activated via ornithine decarboxylase antizyme.Integrin alpha3: An integrin alpha subunit that occurs as alternatively spliced isoforms. The isoforms are differentially expressed in specific cell types and at specific developmental stages. Integrin alpha3 combines with INTEGRIN BETA1 to form INTEGRIN ALPHA3BETA1 which is a heterodimer found primarily in epithelial cells.alpha 1-Antitrypsin Deficiency: Deficiency of the protease inhibitor ALPHA 1-ANTITRYPSIN that manifests primarily as PULMONARY EMPHYSEMA and LIVER CIRRHOSIS.Octoxynol: Nonionic surfactant mixtures varying in the number of repeating ethoxy (oxy-1,2-ethanediyl) groups. They are used as detergents, emulsifiers, wetting agents, defoaming agents, etc. Octoxynol-9, the compound with 9 repeating ethoxy groups, is a spermatocide.Membrane Glycoproteins: Glycoproteins found on the membrane or surface of cells.Receptors, Nicotinic: One of the two major classes of cholinergic receptors. Nicotinic receptors were originally distinguished by their preference for NICOTINE over MUSCARINE. They are generally divided into muscle-type and neuronal-type (previously ganglionic) based on pharmacology, and subunit composition of the receptors.Adrenergic alpha-Agonists: Drugs that selectively bind to and activate alpha adrenergic receptors.Rabbits: The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.Cell Fractionation: Techniques to partition various components of the cell into SUBCELLULAR FRACTIONS.Recombinant Fusion Proteins: Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.Protein Transport: The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.Molecular Weight: The sum of the weight of all the atoms in a molecule.Cations, Divalent: Positively charged atoms, radicals or groups of atoms with a valence of plus 2, which travel to the cathode or negative pole during electrolysis.Lysosomes: A class of morphologically heterogeneous cytoplasmic particles in animal and plant tissues characterized by their content of hydrolytic enzymes and the structure-linked latency of these enzymes. The intracellular functions of lysosomes depend on their lytic potential. The single unit membrane of the lysosome acts as a barrier between the enzymes enclosed in the lysosome and the external substrate. The activity of the enzymes contained in lysosomes is limited or nil unless the vesicle in which they are enclosed is ruptured. Such rupture is supposed to be under metabolic (hormonal) control. (From Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)Gene Expression: The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.PPAR alpha: A nuclear transcription factor. Heterodimerization with RETINOID X RECEPTOR GAMMA is important to metabolism of LIPIDS. It is the target of FIBRATES to control HYPERLIPIDEMIAS.Dinoprost: A naturally occurring prostaglandin that has oxytocic, luteolytic, and abortifacient activities. Due to its vasocontractile properties, the compound has a variety of other biological actions.Cytosol: Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components.Tumor Cells, Cultured: Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.Cell Membrane: The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.Structure-Activity Relationship: The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.DNA Primers: Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.Adrenergic alpha-Antagonists: Drugs that bind to but do not activate alpha-adrenergic receptors thereby blocking the actions of endogenous or exogenous adrenergic agonists. Adrenergic alpha-antagonists are used in the treatment of hypertension, vasospasm, peripheral vascular disease, shock, and pheochromocytoma.Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Glycopeptides: Proteins which contain carbohydrate groups attached covalently to the polypeptide chain. The protein moiety is the predominant group with the carbohydrate making up only a small percentage of the total weight.Isoenzymes: Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Saccharomyces cerevisiae: A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.Kidney: Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.Swine: Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).Hepatocyte Nuclear Factor 1-alpha: Hepatocyte nuclear factor 1-alpha is a transcription factor found in the LIVER; PANCREAS; and KIDNEY that regulates HOMEOSTASIS of GLUCOSE.HeLa Cells: The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.Hydrogen-Ion Concentration: The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)alpha 1-Antichymotrypsin: Glycoprotein found in alpha(1)-globulin region in human serum. It inhibits chymotrypsin-like proteinases in vivo and has cytotoxic killer-cell activity in vitro. The protein also has a role as an acute-phase protein and is active in the control of immunologic and inflammatory processes, and as a tumor marker. It is a member of the serpin superfamily.Biological Transport: The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.Transforming Growth Factor alpha: An EPIDERMAL GROWTH FACTOR related protein that is found in a variety of tissues including EPITHELIUM, and maternal DECIDUA. It is synthesized as a transmembrane protein which can be cleaved to release a soluble active form which binds to the EGF RECEPTOR.Signal Transduction: The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.Gene Expression Regulation: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.Chromatography, High Pressure Liquid: Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.Peptide Fragments: Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.Transcription Factors: Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.Adrenergic alpha-1 Receptor Antagonists: Drugs that bind to and block the activation of ADRENERGIC ALPHA-1 RECEPTORS.alpha Karyopherins: Nucleocytoplasmic transport molecules that bind to the NUCLEAR LOCALIZATION SIGNALS of cytoplasmic molecules destined to be imported into the CELL NUCLEUS. Once attached to their cargo they bind to BETA KARYOPHERINS and are transported through the NUCLEAR PORE COMPLEX. Inside the CELL NUCLEUS alpha karyopherins dissociate from beta karypherins and their cargo. They then form a complex with CELLULAR APOPTOSIS SUSCEPTIBILITY PROTEIN and RAN GTP-BINDING PROTEIN which is exported to the CYTOPLASM.Multienzyme Complexes: Systems of enzymes which function sequentially by catalyzing consecutive reactions linked by common metabolic intermediates. They may involve simply a transfer of water molecules or hydrogen atoms and may be associated with large supramolecular structures such as MITOCHONDRIA or RIBOSOMES.Macromolecular Substances: Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.Tumor Necrosis Factor-alpha: Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.Fibroblasts: Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.Prazosin: A selective adrenergic alpha-1 antagonist used in the treatment of HEART FAILURE; HYPERTENSION; PHEOCHROMOCYTOMA; RAYNAUD DISEASE; PROSTATIC HYPERTROPHY; and URINARY RETENTION.Sequence Alignment: The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.Solubility: The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Spodoptera: A genus of owlet moths of the family Noctuidae. These insects are used in molecular biology studies during all stages of their life cycle.6-Ketoprostaglandin F1 alpha: The physiologically active and stable hydrolysis product of EPOPROSTENOL. Found in nearly all mammalian tissue.Protein Subunits: Single chains of amino acids that are the units of multimeric PROTEINS. Multimeric proteins can be composed of identical or non-identical subunits. One or more monomeric subunits may compose a protomer which itself is a subunit structure of a larger assembly.Disulfides: Chemical groups containing the covalent disulfide bonds -S-S-. The sulfur atoms can be bound to inorganic or organic moieties.Chromatography, Ion Exchange: Separation technique in which the stationary phase consists of ion exchange resins. The resins contain loosely held small ions that easily exchange places with other small ions of like charge present in solutions washed over the resins.Mice, Inbred C57BLDose-Response Relationship, Drug: The relationship between the dose of an administered drug and the response of the organism to the drug.Gene Library: A large collection of DNA fragments cloned (CLONING, MOLECULAR) from a given organism, tissue, organ, or cell type. It may contain complete genomic sequences (GENOMIC LIBRARY) or complementary DNA sequences, the latter being formed from messenger RNA and lacking intron sequences.Mice, Knockout: Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Microscopy, Electron: Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.GTP-Binding Proteins: Regulatory proteins that act as molecular switches. They control a wide range of biological processes including: receptor signaling, intracellular signal transduction pathways, and protein synthesis. Their activity is regulated by factors that control their ability to bind to and hydrolyze GTP to GDP. EC 3.6.1.-.Dogs: The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)Blotting, Western: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.Phosphorylation: The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.Clonidine: An imidazoline sympatholytic agent that stimulates ALPHA-2 ADRENERGIC RECEPTORS and central IMIDAZOLINE RECEPTORS. It is commonly used in the management of HYPERTENSION.Collagen: A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).Promoter Regions, Genetic: DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.Viral Envelope Proteins: Layers of protein which surround the capsid in animal viruses with tubular nucleocapsids. The envelope consists of an inner layer of lipids and virus specified proteins also called membrane or matrix proteins. The outer layer consists of one or more types of morphological subunits called peplomers which project from the viral envelope; this layer always consists of glycoproteins.Yohimbine: A plant alkaloid with alpha-2-adrenergic blocking activity. Yohimbine has been used as a mydriatic and in the treatment of ERECTILE DYSFUNCTION.Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization: A mass spectrometric technique that is used for the analysis of large biomolecules. Analyte molecules are embedded in an excess matrix of small organic molecules that show a high resonant absorption at the laser wavelength used. The matrix absorbs the laser energy, thus inducing a soft disintegration of the sample-matrix mixture into free (gas phase) matrix and analyte molecules and molecular ions. In general, only molecular ions of the analyte molecules are produced, and almost no fragmentation occurs. This makes the method well suited for molecular weight determinations and mixture analysis.Protein Conformation: The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).DNA-Binding Proteins: Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.Cysteine Endopeptidases: ENDOPEPTIDASES which have a cysteine involved in the catalytic process. This group of enzymes is inactivated by CYSTEINE PROTEINASE INHIBITORS such as CYSTATINS and SULFHYDRYL REAGENTS.Interleukin-1: A soluble factor produced by MONOCYTES; MACROPHAGES, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. Interleukin-1 is a general term refers to either of the two distinct proteins, INTERLEUKIN-1ALPHA and INTERLEUKIN-1BETA. The biological effects of IL-1 include the ability to replace macrophage requirements for T-cell activation.DNA: A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).Adrenergic alpha-2 Receptor Agonists: Compounds that bind to and activate ADRENERGIC ALPHA-2 RECEPTORS.Rats, Sprague-Dawley: A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.GTP-Binding Protein alpha Subunits, Gi-Go: A family of heterotrimeric GTP-binding protein alpha subunits that were originally identified by their ability to inhibit ADENYLYL CYCLASES. Members of this family can couple to beta and gamma G-protein subunits that activate POTASSIUM CHANNELS. The Gi-Go part of the name is also spelled Gi/Go.Reverse Transcriptase Polymerase Chain Reaction: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.Ligands: A molecule that binds to another molecule, used especially to refer to a small molecule that binds specifically to a larger molecule, e.g., an antigen binding to an antibody, a hormone or neurotransmitter binding to a receptor, or a substrate or allosteric effector binding to an enzyme. Ligands are also molecules that donate or accept a pair of electrons to form a coordinate covalent bond with the central metal atom of a coordination complex. (From Dorland, 27th ed)Protein Isoforms: Different forms of a protein that may be produced from different GENES, or from the same gene by ALTERNATIVE SPLICING.Adrenergic alpha-2 Receptor Antagonists: Drugs that bind to and block the activation of ADRENERGIC ALPHA-2 RECEPTORS.Time Factors: Elements of limited time intervals, contributing to particular results or situations.Peptide Elongation Factor 1: Peptide elongation factor 1 is a multisubunit protein that is responsible for the GTP-dependent binding of aminoacyl-tRNAs to eukaryotic ribosomes. The alpha subunit (EF-1alpha) binds aminoacyl-tRNA and transfers it to the ribosome in a process linked to GTP hydrolysis. The beta and delta subunits (EF-1beta, EF-1delta) are involved in exchanging GDP for GTP. The gamma subunit (EF-1gamma) is a structural component.Cell Line, Tumor: A cell line derived from cultured tumor cells.Hepatocyte Nuclear Factor 4: A subfamily of nuclear receptors that regulate GENETIC TRANSCRIPTION of a diverse group of GENES involved in the synthesis of BLOOD COAGULATION FACTORS; and in GLUCOSE; CHOLESTEROL; and FATTY ACIDS metabolism.Models, Molecular: Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.Receptors, GABA-A: Cell surface proteins which bind GAMMA-AMINOBUTYRIC ACID and contain an integral membrane chloride channel. Each receptor is assembled as a pentamer from a pool of at least 19 different possible subunits. The receptors belong to a superfamily that share a common CYSTEINE loop.Prostaglandins F: (9 alpha,11 alpha,13E,15S)-9,11,15-Trihydroxyprost-13-en-1-oic acid (PGF(1 alpha)); (5Z,9 alpha,11,alpha,13E,15S)-9,11,15-trihydroxyprosta-5,13-dien-1-oic acid (PGF(2 alpha)); (5Z,9 alpha,11 alpha,13E,15S,17Z)-9,11,15-trihydroxyprosta-5,13,17-trien-1-oic acid (PGF(3 alpha)). A family of prostaglandins that includes three of the six naturally occurring prostaglandins. All naturally occurring PGF have an alpha configuration at the 9-carbon position. They stimulate uterine and bronchial smooth muscle and are often used as oxytocics.Immunohistochemistry: Histochemical localization of immunoreactive substances using labeled antibodies as reagents.Enzyme Activation: Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.Mutagenesis, Site-Directed: Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion.Cell Hypoxia: A condition of decreased oxygen content at the cellular level.Nicotinic Antagonists: Drugs that bind to nicotinic cholinergic receptors (RECEPTORS, NICOTINIC) and block the actions of acetylcholine or cholinergic agonists. Nicotinic antagonists block synaptic transmission at autonomic ganglia, the skeletal neuromuscular junction, and at central nervous system nicotinic synapses.Antibodies, Monoclonal: Antibodies produced by a single clone of cells.Binding, Competitive: The interaction of two or more substrates or ligands with the same binding site. The displacement of one by the other is used in quantitative and selective affinity measurements.GTP-Binding Protein alpha Subunits, Gs: A family of heterotrimeric GTP-binding protein alpha subunits that activate ADENYLYL CYCLASES.Up-Regulation: A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.Nicotinic Agonists: Drugs that bind to and activate nicotinic cholinergic receptors (RECEPTORS, NICOTINIC). Nicotinic agonists act at postganglionic nicotinic receptors, at neuroeffector junctions in the peripheral nervous system, and at nicotinic receptors in the central nervous system. Agents that function as neuromuscular depolarizing blocking agents are included here because they activate nicotinic receptors, although they are used clinically to block nicotinic transmission.Macrophage Inflammatory Proteins: Heparin-binding proteins that exhibit a number of inflammatory and immunoregulatory activities. Originally identified as secretory products of MACROPHAGES, these chemokines are produced by a variety of cell types including NEUTROPHILS; FIBROBLASTS; and EPITHELIAL CELLS. They likely play a significant role in respiratory tract defenses.Receptors, Fibronectin: Specific cell surface receptors which bind to FIBRONECTINS. Studies have shown that these receptors function in certain types of adhesive contact as well as playing a major role in matrix assembly. These receptors include the traditional fibronectin receptor, also called INTEGRIN ALPHA5BETA1 and several other integrins.Mitogen-Activated Protein Kinase 14: A 38-kDa mitogen-activated protein kinase that is abundantly expressed in a broad variety of cell types. It is involved in the regulation of cellular stress responses as well as the control of proliferation and survival of many cell types. The kinase activity of the enzyme is inhibited by the pyridinyl-imidazole compound SB 203580.Fucosyltransferases: Enzymes catalyzing the transfer of fucose from a nucleoside diphosphate fucose to an acceptor molecule which is frequently another carbohydrate, a glycoprotein, or a glycolipid molecule. Elevated activity of some fucosyltransferases in human serum may serve as an indicator of malignancy. The class includes EC 2.4.1.65; EC 2.4.1.68; EC 2.4.1.69; EC 2.4.1.89.Sodium-Potassium-Exchanging ATPase: An enzyme that catalyzes the active transport system of sodium and potassium ions across the cell wall. Sodium and potassium ions are closely coupled with membrane ATPase which undergoes phosphorylation and dephosphorylation, thereby providing energy for transport of these ions against concentration gradients.GTP-Binding Protein alpha Subunits, Gq-G11: A family of heterotrimeric GTP-binding protein alpha subunits that activate TYPE C PHOSPHOLIPASES dependent signaling pathways. The Gq-G11 part of the name is also spelled Gq/G11.Alpha-Globulins: Serum proteins that have the most rapid migration during ELECTROPHORESIS. This subgroup of globulins is divided into faster and slower alpha(1)- and alpha(2)-globulins.Antigens, CD: Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.Mice, Transgenic: Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.GTP-Binding Protein alpha Subunits: The GTPase-containing subunits of heterotrimeric GTP-binding proteins. When dissociated from the heterotrimeric complex these subunits interact with a variety of second messenger systems. Hydrolysis of GTP by the inherent GTPase activity of the subunit causes it to revert to its inactive (heterotrimeric) form. The GTP-Binding protein alpha subunits are grouped into families according to the type of action they have on second messenger systems.Fibronectins: Glycoproteins found on the surfaces of cells, particularly in fibrillar structures. The proteins are lost or reduced when these cells undergo viral or chemical transformation. They are highly susceptible to proteolysis and are substrates for activated blood coagulation factor VIII. The forms present in plasma are called cold-insoluble globulins.

Endometrial lysosomal enzyme activity in normal cycling endometrium. (1/346)

The objective of this study was to evaluate the possible role of four lysosomal enzymes in endometrial function and remodelling during the normal menstrual cycle by fluorimetric measurement (acid phosphatase, N-acetyl-beta-D-glucosaminidase, alpha-L-fucosidase and alpha-D-mannosidase). A prospective study was conducted of 45 endometrial biopsies obtained from women with normal menstrual cycles. Activity of all four enzymes was identified in human endometrium. Activity of acid phosphatase and N-acetyl-beta-D-glucosaminidase was relatively high, whilst that of alpha-L-fucosidase and alpha-D-mannosidase was low. There was no significant change in the activity of any of the four enzymes from the proliferative to the secretory phase of the cycle. This study suggests that the activity of these enzymes remains constant throughout a major portion of the normal cycle.  (+info)

Mid2 is a putative sensor for cell integrity signaling in Saccharomyces cerevisiae. (2/346)

Hcs77 is a putative cell surface sensor for cell integrity signaling in Saccharomyces cerevisiae. Its loss of function results in cell lysis during growth at elevated temperatures (e.g., 39 degrees C) and impaired signaling to the Mpk1 mitogen-activated protein kinase in response to mild heat shock. We isolated the MID2 gene as a dosage suppressor of the cell lysis defect of an hcs77 null mutant. MID2 encodes a putative membrane protein whose function is required for survival of pheromone treatment. Mid2 possesses properties similar to those of Hcs77, including a single transmembrane domain and a long region that is rich in seryl and threonyl residues. We demonstrate that Mid2 is required for cell integrity signaling in response to pheromone. Additionally, we show that Mid2 and Hcs77 serve a redundant but essential function as cell surface sensors for cell integrity signaling during vegetative growth. Both proteins are uniformly distributed through the plasma membrane and are highly O-mannosylated on their extracellular domains. Finally, we identified a yeast homolog of MID2, designated MTL1, which provides a partially redundant function with MID2 for cell integrity signaling during vegetative growth at elevated temperature but not for survival of pheromone treatment. We conclude that Hcs77 is dedicated to signaling cell wall stress during vegetative growth and that Mid2 participates in this signaling, but its primary role is in signaling wall stress during pheromone-induced morphogenesis.  (+info)

Purification and biochemical characterization of two soluble alpha-mannosidases from Candida albicans. (3/346)

Two soluble alpha-mannosidases, E-I and E-II, were purified from C. albicans yeast cells by a three-step procedure consisting of size exclusion and ion exchange chromatographies in Sepharose CL6B and Mono Q columns, respectively, and preparative nondenaturing electrophoresis. E-I and E-II migrated as monomeric polypeptides of 54.3 and 93.3 kDa in SDS-PAGE, respectively. Some biochemical properties of purified enzymes were investigated by using 4-methylumbelliferyl-alpha-D-mannopyranoside and p-nitrophenyl-alpha-D-mannopyranoside as substrates. Hydrolysis of both substrates by either enzyme was optimum at pH 6.0 with 50 mM Mes-Tris buffer and at 42 degrees C. Apparent Kmvalues for hydrolysis of 4-methylumbelliferyl-alpha-D-mannopyranoside and p-nitrophenyl-alpha-D-mannopyranoside by E-I were 0.83 microM and 2. 4 mM, respectively. Corresponding values for E-II were 0.25 microM and 1.86 mM. Swansonine and deoxymannojirimicin strongly inhibited the hydrolysis of 4-methylumbelliferyl-alpha-D-mannopyranoside by both enzymes. On the contrary, hydrolysis of p-nitrophenyl-alpha-D-mannopyranoside by E-I and E-II was slightly stimulated or not affected, respectively, by both inhibitors. E-I and E-II did not depend on metal ions although activity of the latter was slightly stimulated by Mn2+and Ca2+in the range of 0.5-2 mM. At the same concentrations, Mg2+was slightly inhibitory of both enzymes. Substrate specificity experiments revealed that both E-I and E-II preferentially cleaved alpha-1,6 and alpha-1,3 linkages, respectively.  (+info)

Characterization of a soluble class I alpha-mannosidase in human serum. (4/346)

Class I alpha-mannosidases are thought to exist exclusively as integral membrane proteins that play intracellulary an essential role in the N-glycan biosynthesis. Using [3H]Man9GlcNAc2 as a substrate, we were able to identify a soluble alpha-mannosidase in human serum that trims the substrate Man9GlcNAc2 to Man(5-8)GlcNAc2 with Man6GlcNAc2 being the major product. This serum mannosidase is Ca2+-dependent, sensitive to 1-deoxymannojirimycin but insensitive to the class II inhibitor swainsonine and, hence, belongs to class I mannosidases. The enzymatic properties of the serum class I mannosidase are similar to that of the membrane bound class I mannosidases Golgi-mannosidase IA and IB and Man9-mannosidase.  (+info)

Androgen regulation of glycosidase secretion in epithelial cell cultures from human epididymis. (5/346)

The human epididymis and its secretions actively promote sperm fertilizing capacity and provide protection for spermatozoa against harmful influences. Among epididymal secretions, glycosidases have been recently studied and associated with molecular changes on the sperm surface. In the present work, we studied the influence of different concentrations of testosterone, dihydrotestosterone and cyproterone acetate on the secretion of alpha-glucosidase, N-acetyl-glucosaminidase, beta-glucuronidase and alpha-mannosidase by isolated and cultured epithelial cells from human caput, corpus and cauda epididymides. Cell cultures were obtained from aggregates of isolated tubule fragments plated on extracellular matrix-covered multi-well plates. Activities of the glycosidases were measured in conditioned culture media and were higher in the distal regions of the epididymis. Testosterone and dihydrotestosterone significantly increase the enzyme secretion in a concentration-dependent manner. This increase was higher in corpus and/or cauda than in caput epididymis. Cyproterone acetate caused a dose-dependent decrease in glycosidase secretion in cultures from all epididymal regions. It is concluded that the secretion of epididymal glycosidases is regulated by androgen, being stimulated by dihydrotestosterone and testosterone and inhibited by the androgen antagonist cyproterone acetate.  (+info)

Topological and functional characterization of the N-glycans of soybean (Glycine max) agglutinin. (6/346)

Soybean agglutinin (SBA), is a noncovalently bound tetramer comprised of four identical subunits having a single N-glycan chain, Man9GlcNAc2, that is known to be essential for regeneration of the functional tetrameric structure from unfolded subunits. In this study, SBA was found to have strong affinity for concanavalin A, indicating that the N-glycans are extensively solvent-exposed. The susceptibilities of the N-glycans to alpha-mannosidase and endo-beta-N-acetylglucosaminidase revealed that their distal areas have nonreducing ends embedded among the subunits, whereas their proximal regions are solvent-exposed. Endo-beta-N-acetylglucosaminidase-digested SBA was unable to retain its conformation and gradually unfolded. Periodate-oxidized SBA, whose N-glycans closely correspond to the invariant pentasaccharide core, tended to dissociate into the subunits, but permitted to stay as folded monomers. This SBA species was capable of refolding from unfolded subunits but unable to form the functional tetramer. It seems probable that the proximal regions of the N-glycans function in the formation and stabilization of the subunit conformation, whereas the branches outside the invariant cores stabilize the tetrameric structure.  (+info)

Targeted disruption of the lysosomal alpha-mannosidase gene results in mice resembling a mild form of human alpha-mannosidosis. (7/346)

Alpha-mannosidosis is a lysosomal storage disease with autosomal recessive inheritance caused by a deficiency of the lysosomal alpha-mannosidase, which is involved in the degradation of asparagine-linked carbohydrate cores of glycoproteins. An alpha-mannosidosis mouse model was generated by targeted disruption of the gene for lysosomal alpha-mannosidase. Homozygous mutant animals exhibit alpha-mannosidase enzyme deficiency and elevated urinary secretion of mannose-containing oligosaccharides. Thin-layer chromatography revealed an accumulation of oligosaccharides in liver, kidney, spleen, testis and brain. The cellular alterations were characterized by multiple membrane-limited cytoplasmic vacuoles as seen for instance in liver, exocrine pancreas, kidney, thyroid gland, smooth muscle cells, osteocytes and in various neurons of the central and peripheral nervous systems. The morphological lesions and their topographical distribution, as well as the biochemical alterations, closely resemble those reported for human alpha-mannosidosis. This mouse model will be a valuable tool for studying the pathogenesis of inherited alpha-mannosidosis and may help to evaluate therapeutic approaches for lysosomal storage diseases.  (+info)

Purification and properties of major alpha-D-mannosidase in the luminal fluid of porcine epididymis. (8/346)

A lysosomal type alpha-D-mannosidase was successfully purified by DEAE-Sephacel, Red-Amicon and Superdex 200 column chromatographies from porcine cauda epididymal fluid. The purified enzyme consisted of 63 and 51 kDa subunits at equimolar amounts. It cleaved alpha1-2 linked mannosyl residues and less but significantly cleaved alpha1-3 and alpha1-6 linked mannosyl residues in the high-mannose oligosaccharides. The optimal pH to hydrolyze oligosaccharide was in the acidic pH range (pH 3.5 approximately 4.0). Total alpha-D-mannosidase activities in the porcine epididymal fluid increased from proximal to distal caput epididymis, which maintained to cauda epididymis. At least two kinds of alpha-D-mannosidase (lysosomal type enzyme and 135 kDa alpha-D-mannosidase (MAN2B2)) were contained in the porcine epididymal fluid. The activity of the lysosomal type enzyme is much higher than MAN2B2 at the physiological pH. These results suggest that the lysosomal type alpha-D-mannosidase is the predominantly active enzyme in the luminal fluid of porcine epididymis and that it participates in the glycoprotein modification on the sperm surface during epididymal transit.  (+info)

Seeds of Triticale (hybrid of wheat and rye) contain an N-acetylglucosamine specific lectin that was affinity purified in our laboratory (Siva Kumar, N. and Padma, K. (1996) Affinity purification of N-acetyl glucosamine specific lectin. Purification and partial characterization of Triticale lectin. Biochem. Mol. Biol. Int. 38, 1059-1066). Seed extracts also exhibited alpha-mannosidase activity that was isolated by a combination of ion exchange, hydrophobic chromatography and gel filtration. The purified enzyme is a glycoprotein with 7% carbohydrate and exhibited a native molecular mass of 1,95,000 (+/-5000) on Biogel P-200 and dissociated into two major subunits under reducing conditions of molecular masses 58 and 40 kDa, respectively. Both subunits cross-reacted with an antibody to the well-characterized jack bean alpha-mannosidase, suggesting antigenic similarity between the legume and the cereal mannosidases. Purified enzyme binds to Con A-Sepharose gel, possibly through the sugar-binding site.
alpha-Mannosidase (EC 3.2.1.24, alpha-D-mannosidase, p-nitrophenyl-alpha-mannosidase, alpha-D-mannopyranosidase, 1,2-alpha-mannosidase, 1,2-alpha-D-mannosidase, exo-alpha-mannosidase) is an enzyme involved in the cleavage of the alpha form of mannose. Its systematic name is alpha-D-mannoside mannohydrolase.[3][4] ...
In eukaryotes, class I alpha-mannosidases are involved in early N-glycan processing reactions and in N-glycan-dependent quality control in the endoplasmic reticulum (ER). To investigate the role of these enzymes in plants, we identified the ER-type alpha-mannosidase I (MNS3) and the two Golgi-alpha-mannosidase I proteins (MNS1 and MNS2) from Arabidopsis thaliana. All three MNS proteins were found to localize in punctate mobile structures reminiscent of Golgi bodies. Recombinant forms of the MNS proteins were able to process oligomannosidic N-glycans. While MNS3 efficiently cleaved off one selected alpha 1,2-mannose residue from Man(9)GlcNAc(2), MNS1/2 readily removed three alpha 1,2-mannose residues from Man(8)GlcNAc(2). Mutation in the MNS genes resulted in the formation of aberrant N-glycans in the mns3 single mutant and Man(8)GlcNAc(2) accumulation in the mns1 mns2 double mutant. N-glycan analysis in the mns triple mutant revealed the almost exclusive presence of Man(9)GlcNAc(2), ...
Human cytomegalovirus (CMV) is a viral pathogen that infects both genders, who remain asymptomatic unless they receive immunosuppressive medications or acquire infections that trigger reactivation of latent pathogen. to six months. Neutralization titers created in immunized mice are equal to titers discovered clinically after organic infections. This viral vaccine, expressing gB produced from CMV stress […]. ...
Complete information for EDEM2 gene (Protein Coding), ER Degradation Enhancing Alpha-Mannosidase Like Protein 2, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
1PS3: Comparison of Kifunensine and 1-Deoxymannojirimycin Binding to Class I and II alpha-Mannosidases Demonstrates Different Saccharide Distortions in Inverting and Retaining Catalytic Mechanisms
Alpha Mannosidosis is a progressive disorder, and its presence should be suspected in patients with cognitive disabilities, skeletal changes (e.g. swollen joints, curved spine), hearing loss and recurrent infections. Although children with the condition are often born seemingly normal, their condition deteriorates with age. Alpha-mannosidosis can impact on a patients quality of life in many ways, including their ability to live independently, socialise or find employment.[2][7] Generally, phenotypes of alpha-mannosidosis patients are not clearly distinguishable, which makes a prediction of the clinical course for an individual patient challenging.[2] Patients may present to doctors, nurses or health visitors at different stages of progression, and with different ad hoc symptoms, making the link to suspect a diagnosis of alpha-mannosidosis difficult.[2] The main symptoms can also be shared with those of other lysosomal storage disorders, such as mucopolysaccharidosis.[2] Given the progressive ...
[39 Pages Report] Check for Discount on Alpha-Mannosidosis Global Clinical Trials Review, H2, 2016 report by GlobalData. GlobalDatas clinical trial report,
Cytosolic α-mannosidases are glycosyl hydrolases that participate in the catabolism of cytosolic free N-oligosaccharides. Two soluble α-mannosidases (E-I and E-II) belonging to glycosyl hydrolases family 47 have been described in Candida albicans. We demonstrate that addition of pepstatin A during the preparation of cell homogenates enriched α-mannosidase E-I at the expense of E-II, indicating that the latter is generated by proteolysis during cell disruption. E-I corresponded to a polypeptide of 52 kDa that was associated with mannosidase activity and was recognized by an anti-α1,2-mannosidase antibody. The N-mannan core trimming properties of the purified enzyme E-I were consistent with its classification as a family 47 α1,2-mannosidase. Differential density-gradient centrifugation of homogenates revealed that α1,2-mannosidase E-I was localized to the cytosolic fraction and Golgi-derived vesicles, and that a 65 kDa membrane-bound α1,2-mannosidase was present in endoplasmic reticulum and Golgi
The sequence variant c.1010G,A (dbSNP rs1133330) in exon 7 changes argnine in amino acid position 337 with glutamine (p.Arg337Gln). It has a minor allele frequency of 0.26-0.41 in different populations, and is considered a common, benign SNP. Amino acid residue 337 is located on the surface of the MAN2B1 protein, and studies in transfected cells show that p.Gln337 is active and processed correctly. ...
The sequence variant c.2802dupC in exon 22 changes the reading frame, and is, if expressed, expected to result in a non-functional protein (p.Val935ArgfsX120 ...
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ISSN: 2302-8254 Laman Web: http://jfa.ffarmasi.unand.ac.id/ Email: Alamat email ini dilindungi dari robot spam. Anda memerlukan Javascript yang aktif...
Hakcipta Terpelihara. Dilarang mengeluar ulang atau menggunakan mana-mana kandungan laman ini dalam apa jua bentuk dan apa jua cara sama ada elektronik, cetak, mekanik, fotokopi, rakaman atau pengstoran maklumat termasuk sistem dapat kembali tanpa mendapat kebenaran bertulis pemilik hakcipta. ...
Ini laman tidak formal untuk mendekatkan pelajar-pelajar MRSM Kuantan serta bekas-bekas pelajar dengan institusi pendidikan yang menjadi perkebunan ilmu mereka sepanjang pengajian mereka di sini. ...
These cells were scratch-wounded followed by fixation 12-hours post-wounding. Tubulin (Green) and alpha-mannosidase II (Red) were labeled to note cell polarization and Golgi orientation. Cells expressing paxillin lacking LD4 are unable to reorient the Golgi towards the wound edge. From the lab of Christopher Turner, PhD.. Check out our Research Gallery. ...
β-mannosidase (EC 3.2.1.25, MANB) dissects the non-reducing end of N-linked mannose moieties of glycoproteins in eukaryotic cells. The human β-mannosidase gene was amplified by RT-PCR, cloned and sequenced. The DNA sequence was compared with reported human β-mannosidase DNA sequence and sixteen nucleotide differences were found. The deduced amino-acid sequence showed that seven codons coded the same amino acids and nine codons coded different amino acids with reference to nucleotide substitution positions but did not affect recombinant MANB enzyme activity. No splice mutation was observed after comparison with reported MANB DNA sequences. A 75% homology of deduced amino-acid sequence was observed with mouse, goat and bovine β-mannosidase amino-acid sequences. The cloned β-mannosidase gene was subcloned into pET22b+ and pET28a+ expression vectors to transform the BL21-codon plus cells for expression of recombinant MAN22 and MAN28 enzymes, respectively. The optimized conditions for ...
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Diseases caused by the loss of one or more enzymes involved in the hydrolysis of mannoside linkages (MANNOSIDASES). The defects in enzyme activity are primarily associated with genetic mutation of the genes that codes for a particular mannosidase isoenzyme ...
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Produk yang disebut dalam blog/laman web ini tidak boleh digunakan untuk tujuan diagnosis, merawat, menyembuh atau mencegah sebarang penyakit.. Maklumat dalam blog/laman web ini tidak boleh digunakan sebagai pengganti rawatan perubatan dan konsultasi doktor atau pakar pemakanan mahupun dianggap sebagai preskipsi. Sesiapa yang percaya bahawa mereka mengalami sebarang jenis penyakit atau masalah kesihatan, sila berjumpa dengan doktor berkaitan untuk diagnosis dan rawatan susulan. ...
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In terms of performance, users should keep in mind that the warm-up time during a power failure, pull down time, uniformity a ...
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The animals involved in the study are born with a genetic disorder directly analogous to alpha-mannosidosis or AMD, an inherited disease in humans that causes severe mental retardation and skeletal abnormalities. Cats with AMD do not live more than six months. Children born with the worst form of the disease rarely survive into their teens. "Through gene therapy, we replace a broken gene responsible for alpha-mannosidase with the correct, functioning copy, to dramatic results," said John H. Wolfe, a professor of pathology and medical genetics at the Penn School of Veterinary Medicine and a neurology researcher at Children s Hospital. "The treated cats were markedly improved compared to diseased cats, with better balance and muscle control and fewer tremors ...
A recent study in Genetics in Medicine aiming at the identification by whole-exome sequencing of genes involved in infantile nystagmus found heterozygous missense mutations in the MANBA gene, which the encodes lysosomal beta-mannosidase. The mutations resulted in decrease of ?-mannosidase activities in the patients as well as in mutant-transfected HEK293T cells. MANBA is expressed in the pretectal nucleus of the developing midbrain, […]. ...
These cells were scratch-wounded followed by fixation 12-hours post-wounding. Tubulin (Green) and alpha-mannosidase II (Red) were labeled to note cell polarization and Golgi orientation. Cells expressing paxillin lacking LD4 are unable to reorient the Golgi towards the wound edge. From the lab of Christopher Turner, PhD.. Check out our Research Gallery. ...
These cells were scratch-wounded followed by fixation 12-hours post-wounding. Tubulin (Green) and alpha-mannosidase II (Red) were labeled to note cell polarization and Golgi orientation. Cells expressing paxillin lacking LD4 are unable to reorient the Golgi towards the wound edge. From the lab of Christopher Turner, PhD.. Check out our Research Gallery. ...
NORDIN, JH and Kunkel, JG, "SIZE HETEROGENEITY OF THE HIGH MANNOSE OLIGOSACCHARIDES OF INSECT VITELLINS" (1982). FEDERATION PROCEEDINGS. 316 ...
TY - JOUR. T1 - Cobalt ions cross an electrotonic synapse if cytoplasmic concentration is low. AU - Politoff, A.. AU - Pappas, G. D.. AU - Bennett, Michael V. L.. PY - 1974/8/16. Y1 - 1974/8/16. UR - http://www.scopus.com/inward/record.url?scp=0016138697&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0016138697&partnerID=8YFLogxK. U2 - 10.1016/0006-8993(74)90466-1. DO - 10.1016/0006-8993(74)90466-1. M3 - Article. C2 - 4844459. AN - SCOPUS:0016138697. VL - 76. SP - 343. EP - 346. JO - Brain Research. JF - Brain Research. SN - 0006-8993. IS - 2. ER - ...
Teringat dulu saya hampir terlupa tentang ini..bukan tidak tahu tapi jarang diamalkan oleh masyarakat Malaysia termasuklah saya..jadi sebelum makan ubat baca bismillah dan doa. Saya tersedar ketika menghadiri satu kuliah agama di mana ustaz tu mengingatkan kembali pergantungan kita kepada Allah. Ustaz kata bagusnya kalau pergi di mana sahaja semua mengingatkan kita kepada Allah, seperti kos farmasi ketika nak bagi ubat kat pesakit, perlu cakap Allah yang menyembuhkan,ubat ini sebagai usaha kita, kos kejuteraan contohnya kejuteraan mekanikal..mesin dicipta mendapat perhatian seluruh dunia bagi memudahkan pekerjaan manusia seharian tetapi pencipta mesin iaitu jurutera itu berkata pada wartawan atau pada seluruh ummat bahawa mesin yang dicipta tak dapat menandingi kehebatan Allah dan bidang kejuruteraan kimia membuktikan zarah-zarah di udara bergerak membentuk kalimah Allah, begitu juga air yang bila mana dibaca kalimah yang baik seperti kalimah Allah terhasillah rupa bentuk zarah air yang indah ...
PERHATIAN: Ini adalah laman web peribadi. Tiada kaitan dengan Syarikat MLM yang disertai. Tujuan laman web ini disediakan adalah bagi membantu ahli-ahli yang berdaftar di bawah kumpulan AHB Cordyceps4u untuk membuat promosi menaja ahli-ahli yang baru. Promosi boleh dilakukan secara online dan juga offline serta terpulang kepada kreativiti anda ...
Ini website bolasepak Indie dan ditulis dengan nada sinis dan humor. Kalau rasa sakit bontot team korang kena menganjing pasal dah 2 dekad tak menang liga sila ke SANDARKANPADAKENANGAN.COM. Kalau sakit bontot pasal team korang kena maki team kanak-kanak Taski Wenger sila la ke website lain juga. Kalau nak serius jugak sila angkat kaki, jalan terus ,belok kiri le lepas tu sila pergi mampos ...
Meletakkan hukum syariat yang berlaku bagi semua manusia dalam bidang ibadah, muamalah dan segenap urusan mereka, memutuskan persengketaan dan perseteruan di antara mereka adalah hak mutlak Allah Taala sebagai pencipta manusia. Allah Taala berfirman, "Ingatlah, menciptakan dan memerintah hanyalah hak Allah." (Al-Araf: 59).. Sebagai pencipta, secara otomatis Allah mengetahui ciptaanNya, Allah mengetahui manusia sebagai hambaNya, apa yang baik dan apa yang buruk bagi mereka, sehingga Dia meletakkan tatanan dan aturan sebagai konsekuensi dari rububiyahNya dan manusia patut menerima tatanan dan aturan tersebut sebagai tuntutan dari penghambaan dan demi kemaslahatan mereka sendiri.. "Tentang sesuatu apapun kamu berselisih, maka putusannya (terserah) kepada Allah. Itulah Allah Tuhanku, kepadaNya lah aku bertawakkal dan kepadaNyalah aku kembali." (Asy-Syura: 10).. Karena hak menetapkan syariat hanya di tangan Allah, maka tidak pantas bagi siapa pun untuk mengakui hak tersebut, baik dengan ...
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Perkataan Millat adalah bahasa Urdu yang biasa digunakan untuk merujuk kepada negara. Laman web itu sudah mempunyai 4,300 ahli selepas tiga hari dilancarkan, kebanyakannya warga Pakistan yang boleh berbahasa Inggeris dalam lingkungan usia 20-an ...
Perkataan Millat adalah bahasa Urdu yang biasa digunakan untuk merujuk kepada negara. Laman web itu sudah mempunyai 4,300 ahli selepas tiga hari dilancarkan, kebanyakannya warga Pakistan yang boleh berbahasa Inggeris dalam lingkungan usia 20-an ...
Blog ini adalah hak milik peribadi saya dan tidak ditaja oleh mana-mana warga persendirian, organisasi, mahupun pihak ketiga.. Penafian: Laman web ini tidak bertujuan untuk menggantikan nasihat profesional, diagnosis, atau rawatan oleh pakar perubatan berlesen. Produk Shaklee tidak bertujuan untuk membuat diagnosis, merawat, menyembuh atau mencegah sebarang penyakit. Jika anda memerlukan sebarang nasihat berkaitan perubatan, hubungi doktor anda dengan segera.. ...
Edit 31/10/2012 : Gara-gara post ni yang bertajuk Seriusss..rinduuu ada yang bertanya .. dengan siapa CF bercinta? hahaha gelak bes00 CF dibuatnyee.. bercinta dengan BLOG ni lah.. blog ni lah terapi buat CF.. suka duka bersama.. hari ni 800 post dah terhasil di laman blog ni .. semoga kita dapat berkongsi ilmu bersama ...
Pemilik blog ini tidak bertanggungjawab sekiranya berlaku kerosakan atau masalah pada system komputer pelawat semasa/sesudah melawat laman ini. Juga tidak akan bertanggungjawab terhadap kerosakan hartabenda pelawat sekiranya pelawat mencuba tutorial atau apa apa sahaja yang dipersembahkan dalam blog ini. Segala tulisan dan pandangan dalam blog ini adalah telus. Segala artikel2 atau bahan2 info adalah dipetik dari berbagai2 sumber dan disiarkan sebagai rujukan semata mata.Penulis terlebih dahulu memohon ampun dan maaf sekiranya ada antara artikel atau tulisan yang menyentuh/menganggu emosi para pelawat. Ucapan terima kasih kepada pihak sumber kerana segala rujukan adalah amat bermanafaat kepada kami , Peminat Pertanian ...
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Admin blog ini tidak akan bertanggungjawab ke atas sebarang kerugian, kehilangan atau kerosakan yang dialami dalam penggunaan sebarang maklumat yang dicapai daripada laman blog ini. Terima kasih. ...
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Metaglip Le Moins Cher. bien être, comme lamitriptyline, on peut en effet avoir limpression quil y a plusieurs noyaux alors quen réalité il y a plusieurs lobes, sont dans ce dossier. Avec, Metaglip Le Moins Cher fais 2 test de grossesse positif Metaglip Le Moins Cher une prise de sang et je suis bien enceinte, publié le 031016 Blog Cours particuliers Donner des Cours Particuliers Qui Peut Donner des Cours Particuliers, à comprimer et à soulever le membre, répondit lhomme-arbre. «Voyez grand. Possible de faire un article special cuisson du poulet. toutcomment! Les labernois choisis pour assister les humains sont des chiens possédant des qualités exceptionnelles, il est indispensable de garantir des conditions de concurrence égales entre les différents États membres.. Des ampoules et ] Aujourdhui, en raison que Mme Schiappa doive partir, ce qui évidente ( ne râle t-on pas, parce Metaglip Le Moins Cher avant un traitement thrombolytique par retéplase, Metaglip Le Moins Cher. ...
... alpha-mannosidase (EC 3.2.1.113). Alpha-mannosidase is involved in the maturation of Asn-linked oligosaccharides. The enzyme ... "Isolation and expression of murine and rabbit cDNAs encoding an alpha 1,2-mannosidase involved in the processing of asparagine- ... hydrolyses terminal 1,2-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide man(9)(glcnac)(2) in a calcium- ...
Glycoprotein endo-alpha-1,2-mannosidase is an enzyme that in humans is encoded by the MANEA gene. GRCh38: Ensembl release 89: ... Spiro MJ, Bhoyroo VD, Spiro RG (1997). "Molecular cloning and expression of rat liver endo-alpha-mannosidase, an N-linked ... MANEA mannosidase, endo-alpha". Land A, Braakman I (2001). "Folding of the human immunodeficiency virus type 1 envelope ...
... alpha-mannosidase (EC 3.2.1.24) (EC 3.2.1.114). Lysosomal alpha-mannosidase is necessary for the catabolism of N-linked ... non-reducing alpha-D-mannose residues in alpha-D-mannosides, and can cleave all known types of alpha-mannosidic linkages. ... The domain is predominantly found in the enzyme alpha-mannosidase. Henrissat B, Callebaut I, Mornon JP, Fabrega S, Lehn P, ... "The structure of bovine lysosomal alpha-mannosidase suggests a novel mechanism for low-pH activation". J. Mol. Biol. 327 (3): ...
Epididymis-specific alpha-mannosidase precursor Mannosidase alpha class 2B member core-specific lysosomal alpha-1,6-Mannosidase ... epididymis-specific alpha-mannosidase mannosidase, alpha, class 2B, member GRCh38: Ensembl release 89: ENSG00000013288 - ... "Entrez Gene: MAN2B2 mannosidase, alpha, class 2B, member 2". Land A, Braakman I (2001). "Folding of the human immunodeficiency ... Epididymis-specific alpha-mannosidase is an enzyme that in humans is encoded by the MAN2B2 gene. ...
"Entrez Gene: MAN1B1 mannosidase, alpha, class 1B, member 1". Land A, Braakman I (2001). "Folding of the human immunodeficiency ... Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase is an enzyme that in humans is encoded by the MAN1B1 gene ... Tremblay LO, Herscovics A (Nov 1999). "Cloning and expression of a specific human alpha 1,2-mannosidase that trims Man9GlcNAc2 ... 2-mannosidase gene involved in N-glycan maturation". Glycobiology. 8 (6): 585-95. doi:10.1093/glycob/8.6.585. PMID 9592125. ...
"Entrez Gene: MAN1A1 mannosidase, alpha, class 1A, member 1". Land A, Braakman I (2001). "Folding of the human immunodeficiency ... Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA is an enzyme that in humans is encoded by the MAN1A1 gene. This gene encodes ... 1997). "Man9-mannosidase from pig liver is a type-II membrane protein that resides in the endoplasmic reticulum. cDNA cloning ... Bieberich E, Bause E (1995). "Man9-mannosidase from human kidney is expressed in COS cells as a Golgi-resident type II ...
"Molecular cloning and expression of cDNAs encoding human alpha-mannosidase II and a previously unrecognized alpha-mannosidase ... Alpha-mannosidase 2 is an enzyme that in humans is encoded by the MAN2A1 gene. This gene encodes a protein which is a member of ... "Entrez Gene: MAN2A1 mannosidase, alpha, class 2A, member 1". Land A, Braakman I (Aug 2001). "Folding of the human ... Moremen KW, Touster O, Robbins PW (Sep 1991). "Novel purification of the catalytic domain of Golgi alpha-mannosidase II. ...
"Entrez Gene: MAN1A2 mannosidase, alpha, class 1A, member 2". Land A, Braakman I (2001). "Folding of the human immunodeficiency ... Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB is an enzyme that in humans is encoded by the MAN1A2 gene. GRCh38: Ensembl ... 2-mannosidase gene involved in N-glycan maturation". Glycobiology. 8 (6): 585-95. doi:10.1093/glycob/8.6.585. PMID 9592125. " ...
ER degradation-enhancing alpha-mannosidase-like 1 is an enzyme that in humans is encoded by the EDEM1 gene. GRCh38: Ensembl ... "Entrez Gene: EDEM1 ER degradation enhancer, mannosidase alpha-like 1". Nagase T, Seki N, Ishikawa K, et al. (1997). "Prediction ... 2001). "A novel ER alpha-mannosidase-like protein accelerates ER-associated degradation". EMBO Rep. 2 (5): 415-22. doi:10.1093/ ...
ER degradation-enhancing alpha-mannosidase-like 3 is an enzyme that in humans is encoded by the EDEM3 gene. GRCh38: Ensembl ... "Entrez Gene: EDEM3 ER degradation enhancer, mannosidase alpha-like 3". Maruyama K, Sugano S (1994). "Oligo-capping: a simple ...
ER degradation-enhancing alpha-mannosidase-like 2 is an enzyme that in humans is encoded by the EDEM2 gene. GRCh38: Ensembl ... "Entrez Gene: EDEM2 ER degradation enhancer, mannosidase alpha-like 2". Andersson B, Wentland MA, Ricafrente JY, et al. (1996 ...
EDEM2: ER degradation-enhancing alpha-mannosidase-like 2. *EDN3: endothelin 3. *ENTPD6: Ectonucleoside triphosphate ...
Disruption of Golgi alpha-mannosidase II with swainsonine induces hybrid-type glycans. These glycans have a Man5GlcNAc2 core ... It is a potent inhibitor of Golgi alpha-mannosidase II, an immunomodulator, and a potential chemotherapy drug. As a toxin in ...
"Alpha-mannosidase-II deficiency results in dyserythropoiesis and unveils an alternate pathway in oligosaccharide biosynthesis ...
... shows no inhibitory action against mannosidase II or the endoplasmic reticulum alpha-mannosidase, and it weakly ... Kifunensine does not inhibit mannosidase II or the endoplasmic reticulum alpha-mannosidase. It weakly inhibits arylmannosidase ... Kifunensine inhibits human endoplasmic reticulum α-1,2-mannosidase I and Golgi Class I mannosidases IA, IB and IC with Ki ... Kifunensine is a potent inhibitor of the mannosidase I enzyme. It is 50 to 100 times more potent than deoxymannojirimycin - an ...
MAN1A2: Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB. *MEAF6: MYST/ESA1 associated factor 6 ...
Champion MJ, Brown JA, Shows TB (1979). "Assignment of cytoplasmic alpha-mannosidase (MANA) and confirmation of mitochondrial ... a partially unraveled alpha helix in the semi open form, and an alpha helix in the active form. The mitochondrial form of IDH2 ... Its main function is to catalyze the oxidative decarboxylation of isocitrate into alpha-ketoglutarate. Human isocitrate ... NADP+-dependent isocitrate dehydrogenase enhances the sensitivity of HeLa cells toward tumor necrosis factor-alpha and ...
H is able to cleave each structure of this oligosaccharide as it is processed until the enzyme Golgi alpha-mannosidase II ...
This domain occurs within alpha-1,2-mannosidases, which remove alpha-1,2-linked mannose residues from Man(9)(GlcNAc)(2) by ... 6-mannosidase EC 3.2.1.-, α-mannosidase EC 3.2.1.24, α-1,2-mannosidase EC 3.2.1.-, α-1,3-mannosidase EC 3.2.1.- and α-1,4- ... Glycoside hydrolase family 92 includes enzymes with mannosyl-oligosaccharide α-1,2-mannosidase EC 3.2.1.113, mannosyl- ... mannosidase EC 3.2.1.- activities. It includes enzymes critical for the maturation of N-linked oligosaccharides and ER- ...
In addition, B. longum can uniquely ferment galactomannan-rich natural gum using glucosaminidases and alpha-mannosidases that ...
B. Acid alpha-mannosidase activity Diagnosis is confirmed by measuring residual alpha-mannosidase activity in leukocytes or ... a b c d e f g h i j k l m n o p q r s t u v w x y z aa ab Malm D, Nilssen O (2008). "Alpha-mannosidosis". Orphanet J Rare Dis. ... a b c d e f g h Borgwardt L, Lund AM, Dali CI (2014). Alpha-mannosidosis - a review of genetic, clinical findings and options ... "Missense and nonsense mutations in the lysosomal alpha-mannosidase gene (MANB) in severe and mild forms of alpha-mannosidosis" ...
alpha-Mannosidase na US National Library of Medicine Medical Subject Headings (MeSH) ... Liberation of D-mannose from various glycoproteins by α-mannosidase isolated from jack bean meal". J. Biol. Chem. 241 (4): 1010 ... Winchester, B. (1984). "Role of α-D-mannosidases in the biosynthesis and catabolism of glycoproteins". Biochem. Soc. Trans. 12 ... "Alpha-mannosidosis.". Orphanet J Rare Dis 3: 21. PMC 2515294. PMID 18651971. doi:10.1186/1750-1172-3-21. ...
Alpha-mannosidase 2 MASS1: monogenic, audiogenic seizure susceptibility 1 homolog (mouse) MCC: Colorectal mutant cancer protein ... Prolyl 4-hydroxylase subunit alpha-2 PCBD2: Pterin-4-alpha-carbinolamine dehydratase 2 PELO: Pelota homolog PHAX: ... alpha interacting protein (synphilin) SPEF2: Sperm flagellar protein 2 SPINK5: serine protease inhibitor Kazal-type 5 (LEKTI) ... alpha-induced protein 8 TTC37: Tetratricopeptide repeat domain 37 UPF0488: encodes G protein-coupled receptor protein signaling ...
... a nervous tissue staining technique Golgi alpha-mannosidase II, an enzyme Golgi cell, a type of interneuron found in the ...
Beta-mannosidase Alpha-mannosidosis "Mannosidosis, beta A, lysosomal , Genetic and Rare Diseases Information Center (GARD) - an ... Beta-mannosidosis, also called lysosomal beta-mannosidase deficiency, is a disorder of oligosaccharide metabolism caused by ... Beta-mannosidase function is consistent with: A diagnosis of beta-mannosidosis is suspected based on the persons clinical ... if one first looks at the normal function of beta-mannosidase such as its function of breaking down disaccharides[medical ...
mannosidase *alpha-Mannosidase. *beta-mannosidase. *Aspartylglucosaminidase. *Fucosidase. *NAGA. Transport. *SLC17A5. M6P ...
Purification and characterization of a rat liver Golgi alpha-mannosidase capable of processing asparagine-linked ...
Mannosidase , Diseases, Mannosidase Deficiency , Mannosidase Deficiency Disease , Mannosidase Deficiency Syndrome , Mannosidase ... alpha-Mannosidosis beta-Mannosidosis associated chemicals · associated genes · associated diseases · associated exposure ... Mannosidase Deficiency Diseases Synonyms Deficiency Disease, Mannosidase , Deficiency Diseases, Mannosidase , Deficiency ... Mannosidase Deficiency Diseases 3.. Diseases ← Nutritional and Metabolic Diseases ← Metabolic Diseases ← Metabolism, Inborn ...
... alpha-D-mannosidase, p-nitrophenyl-alpha-mannosidase, alpha-D-mannopyranosidase, 1,2-alpha-mannosidase, 1,2-alpha-D-mannosidase ... GeneReviews/NCBI/NIH/UW entry on Alpha-Mannosidosis OMIM entries on Alpha-Mannosidosis alpha-Mannosidase at the US National ... exo-alpha-mannosidase) is an enzyme involved in the cleavage of the alpha form of mannose. Its systematic name is alpha-D- ... Humans express the following three alpha-mannosidase isozymes: It can be utilized in experiments that determine the effects of ...
Mannan endo-1,6-alpha-mannosidase (EC 3.2.1.101, exo-1,6-beta-mannanase, endo-alpha-1-,6-D-mannanase, endo-1,6-beta-mannanase, ... mannan endo-1,6-beta-mannosidase, 1,6-alpha-D-mannan mannanohydrolase) is an enzyme with systematic name 6-alpha-D-mannan ... Mannan endo-1,6-alpha-mannosidase at the US National Library of Medicine Medical Subject Headings (MeSH) ... Random hydrolysis of (1-,6)-alpha-D-mannosidic linkages in unbranched (1-,6)-mannans. References[edit]. *^ Nakajima, T.; Maitra ...
van den Elsen, J.M., Kuntz, D.A., Rose, D.R., "Structure of Golgi alpha-mannosidase II: a target for inhibition of growth and ... Golgi α-mannosidase II is a key enzyme involved in N-linked Glycan processing. It is inhibited by small molecule swainsonine. ...
alpha-1,2-Mannosidase. Primary source. WormBase:WBGene00008411 Locus tag. CELE_D2030.1 Gene type. protein coding. RNA name ... alpha-1,2-Mannosidase. RefSeq status. REVIEWED. Organism. Caenorhabditis elegans (strain: Bristol N2) Lineage. Eukaryota; ... mans-1 alpha-1,2-Mannosidase [ Caenorhabditis elegans ] Gene ID: 172510, updated on 12-Aug-2018 ... mannosyl-oligosaccharide 1,2-alpha-mannosidase activity IBA Inferred from Biological aspect of Ancestor. more info ...
"Molecular cloning and expression of cDNAs encoding human alpha-mannosidase II and a previously unrecognized alpha-mannosidase ... "Molecular cloning and expression of cDNAs encoding human alpha-mannosidase II and a previously unrecognized alpha-mannosidase ... "Molecular cloning and expression of cDNAs encoding human alpha-mannosidase II and a previously unrecognized alpha-mannosidase ... Alpha-mannosidase 2x (Mannosidase, alpha, class 2A, member 2, isoform CRA_f) ...
alpha-1,2-MannosidaseSequence analysis. Automatic assertion according to sequence analysisi ... alpha-1,2-MannosidaseUniRule annotation. Automatic assertion according to rulesi ... tr,Q9P8T8,Q9P8T8_HYPJE alpha-1,2-Mannosidase OS=Hypocrea jecorina OX=51453 GN=MDS1 PE=1 SV=1 ...
... alpha-D-mannosidase, p-nitrophenyl-alpha-mannosidase, alpha-D-mannopyranosidase, 1,2-alpha-mannosidase, 1,2-alpha-D-mannosidase ... exo-alpha-mannosidase) is an enzyme involved in the cleavage of the alpha form of mannose. Its systematic name is alpha-D- ... File:Tonge redo alpha mannosidase.png Cartoon depiction of the protein Streptococcus pyogenes family GH38 α-Mannosidase created ... alpha-Mannosidase at the US National Library of Medicine Medical Subject Headings (MeSH) ...
2 Mannosidase IA antibody. Validated in WB, ICC/IF and tested in Mouse, Rat, Human. Cited in 1 publication(s). Independently ... Anti-alpha 1,2 Mannosidase IA antibody. See all alpha 1,2 Mannosidase IA primary antibodies. ... Anti-alpha 1,2 Mannosidase IA antibody (ab51262) at 1/1000 dilution + Human colon whole cell lysate at 40 µg. Secondary. HRP- ... Western blot - Anti-alpha 1,2 Mannosidase IA antibody (ab51262)This image is courtesy of an Abreview submitted by Praveen ...
View mouse Man1b1 Chr2:25332730-25352213 with: sequences, polymorphisms, proteins, references, function, expression
View and select products within our alpha-mannosidase Inhibitors category available for immediate purchase. ... ChemCruz™ Chemical alpha-mannosidase Substrates for functional analysis of cellular responses to alpha-mannosidase ... Phenyl 2,3,4,6-Tetra-O-acetyl-1-thio-alpha-D-mannopyranoside A mannose analog that acts as an inhibitor of a-mannosidase 108032 ... alpha-mannosidase Inhibitors. Autophagy is a membrane trafficking mechanism that delivers cytoplasmic cargo to the vacuole/ ...
2-mannosidase reveals the basis for differences in specificity of the endoplasmic reticulum and Golgi class I enzymes. ... All alpha proteins alpha/alpha toroid Seven-hairpin glycosidases Class I alpha-1;2-mannosidase, catalytic domain Class I alpha- ... All alpha proteins alpha/alpha toroid Seven-hairpin glycosidases Class I alpha-1;2-mannosidase, catalytic domain Class I alpha- ... Mannosyl-oligosaccharide alpha-1,2-mannosidase (1KRF:A,B) * Mannosyl Oligosaccharide 1 2 Alpha Mannosidase Activity ...
alpha-Mannosidase from E indica exhibits immunological identity with alpha-mannosidase from Canavalia ensiformis but not with ... alpha-mannosidase from Erythrina indica seeds is a Zn(2+) dependent glycoprotein with 8.6% carbohydrate. The enzyme has a ... Characterization of alpha-mannosidase from Erythrina indica seeds and influence of endogenous lectin on its activity.. Kestwal ... Incubation of E. indica seed lectin with alpha-mannosidase resulted in 35% increase in its activity, while no such activation ...
2-mannosidase, catalytic domain family. Additional information, provided for both this family and the superfamily it belongs to ... The SCOP classification for the Class I alpha-1; ... Class I alpha-1;2-mannosidase, catalytic domain family. SCOP ... Mannosyl-oligosaccharide 1,2-alpha-mannosidase. 0. 2.929. --. DIRECT. Enzyme Commission (EC). Hydrolases. 0. 0.4043. Least ... Mannosyl-oligosaccharide 1,2-alpha-mannosidase. 0. 2.741. --. DIRECT. Enzyme Commission (EC). Glycosidases, i.e. enzymes ...
HomeAlpha-mannosidase-II deficiency results in dyserythr. Alpha-mannosidase-II deficiency results in dyserythr. By admin ...
We have examined the expression of one of these genes, alpha-mannosidase. Using an alpha-mannosidase cDNA probe in Northern ( ... in alpha-mannosidase enzyme-specific activity during development is due to an increase in the levels of alpha-mannosidase mRNA ... However, cell density did play a major role in the expression of alpha-mannosidase. High cell density or the presence of buffer ... Finally, the alpha-mannosidase gene was not expressed in aggregation-negative mutant strain HMW 404. ...
The plasma alpha-mannosidase assay is a rapid and reliable screening test for the mannosidosis genotype and for detecting ... The plasma alpha-mannosidase assay is a rapid and reliable screening test for the mannosidosis genotype and for detecting ... The plasma alpha-mannosidase assay is a rapid and reliable screening test for the mannosidosis genotype and for detecting ... The plasma alpha-mannosidase assay is a rapid and reliable screening test for the mannosidosis genotype and for detecting ...
Definition of Aryl-alpha-mannosidase with photos and pictures, translations, sample usage, and additional links for more ... Act on p-nitrophenyl-alpha-d-mannopyranoside Registry number: EC 3.2.1.- Synonym: aryl-alpha-mannosidase a, aryl-alpha- ... Aryl-alpha-mannosidase Images Lexicographical Neighbors of Aryl-alpha-mannosidase. arvels. arvicole. arvicoles. arviculture. ... Aryl-alpha-mannosidase Pictures. Click the following link to bring up a new window with an automated collection of images ...
38, 1059-1066). Seed extracts also exhibited alpha-mannosidase activity that was isolated by a combination of ion exchange, ... Both subunits cross-reacted with an antibody to the well-characterized jack bean alpha-mannosidase, suggesting antigenic ... similarity between the legume and the cereal mannosidases. Purified enzyme binds to Con A-Sepharose gel, possibly through the ... Alpha-mannosidase from the seeds of Triticale.. *. A Subha Mahadevi, R Vegiraju Suryanarayana, Nadimpalli Siva Kumar ...
Cleaves alpha 1,2-, alpha 1,3-, and alpha 1,6-linked mannose residues from glycoproteins. Involved in the degradation of free ...
Molecular cloning and expression of cDNAs encoding human alpha-mannosidase II and a previously unrecognized alpha-mannosidase ... Oligosaccharide side-chains of HIV-1 gp160 are processed by glycosidase I and II, mannosidase I and II, acetylglucosaminyl ... Oligosaccharide side-chains of HIV-1 gp160 are processed by glycosidase I and II, mannosidase I and II, acetylglucosaminyl ... Oligosaccharide side-chains of HIV-1 gp160 are processed by glycosidase I and II, mannosidase I and II, acetylglucosaminyl ...
... Inhibition of match element 5 (C5) reduced myocardial infarction in pet November 22, 2018, admin Alpha- ... August 14, 2018, admin Alpha-Mannosidase, In developed countries, like the UK and america, between 15% and 20% of the populace ... January 9, 2018, admin Alpha-Mannosidase, Hepatitis C Pathogen (HCV) is a serious global wellness issue, infecting almost 3% of ... October 14, 2017, admin Alpha-Mannosidase, analysis of biomarkers is highly desirable in molecular pathology since it allows ...
Demonstration of deficient alpha-mannosidase enzyme activity is considered the gold standard to confirm a diagnosis of alpha- ... Alpha mannosidosis is an autosomal recessive lysosomal storage disorder caused by a deficiency of alpha-mannosidase enzyme ... This biochemical test is a quantitative measurement of alpha-mannosidase enzyme activity and can be used as a 1st tier test for ... patients with a clinical suspicion of alpha-mannosidosis. ... Alpha-mannosidosis : Alpha-mannosidase Enzyme Analysis. PRINT ...
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  • Using an alpha-mannosidase cDNA probe in Northern (RNA) blot analysis, we have shown that the previously observed increase in alpha-mannosidase enzyme-specific activity during development is due to an increase in the levels of alpha-mannosidase mRNA. (asm.org)
  • Northern (RNA) analysis of poly(A)+ mRNA from normal, G.C., and other unrelated HEMPAS cells all showed double bands at the 7.6-kilobase position, detected by an alpha-ManII cDNA probe, but expression of these bands in G.C. cells was substantially reduced (less than 10% of normal). (pnas.org)
  • In Southern analysis of G.C. and normal genomic DNA, the restriction fragment patterns detected by the alpha-ManII cDNA probe were indistinguishable. (pnas.org)
  • Fucosidosis is a rare genetic disorder characterized by deficiency of the enzyme alpha-L-fucosidase, which is required to break down (metabolize) certain complex compounds (e.g., fucose-containing glycolipids or fucose-containing glycoproteins). (rarediseases.org)
  • EfMan-I catalyzes the cleavage of alpha 1-2-mannose from not only oligomannoses but also high-mannose-type N-glycans on glycoproteins. (qdio.ac.cn)
  • A similar rate of hydrolysis of isomaltose by both isozymes was found indicating that the reduced catalytic activity of the variant isozyme toward glycogen is not the result of a reduced ability of this enzyme to cleave the alpha-1,6 linkages of glycogen. (worldwidescience.org)
  • Under physiological conditions, clusterin is modified in the endoplasmic reticulum (ER), and proteolytically cleaved in the Golgi to generate discrete alpha and beta chains prior to secretion. (nih.gov)
  • In humans, alpha-mannosidosis results in progressive facial- and skeletal abnormalities, motor impairment, hearing impairment, intellectual disability, recurrent infections and immune deficiency. (symptoma.com)
  • We have examined the expression of one of these genes, alpha-mannosidase. (asm.org)
  • High cell density or the presence of buffer conditioned by high-density cells was sufficient to induce expression of alpha-mannosidase, indicating that this is one of the prestarvation response genes. (asm.org)
  • Patients may present to doctors, nurses or health visitors at different stages of progression, and with different ad hoc symptoms, making the link to suspect a diagnosis of alpha-mannosidosis difficult. (wikipedia.org)
  • A deficiency can lead to alpha-mannosidosis. (wikipedia.org)
  • The plasma alpha-mannosidase assay is a rapid and reliable screening test for the mannosidosis genotype and for detecting carrier animals. (monash.edu)
  • Alpha-mannosidosis (AM) is a lysosomal storage disease , which may also be referred to as lysosomal acid hydrolase alpha-mannosidase or LAHAM. (symptoma.com)
  • Recently, a successful bone marrow transplantation (BMT) in an alpha-mannosidosis patient was reported. (symptoma.com)
  • Defects in this gene have been associated with lysosomal alpha-mannosidosis. (genecards.org)
  • Alpha-mannosidosis is a lysosomal storage disorder , first described by Swedish physician Okerman in 1967. (wikipedia.org)
  • Facial features in alpha-mannosidosis. (wikipedia.org)
  • Alpha-mannosidosis is a lifelong multi-systemic progressive disease, with neuromuscular and skeletal deterioration over decades. (wikipedia.org)
  • Depending on the severity of the disease, alpha-mannosidosis has been classified into three proposed subtypes, based on severity and age of onset. (wikipedia.org)
  • Alpha Mannosidosis is a progressive disorder, and its presence should be suspected in patients with cognitive disabilities, skeletal changes (e.g. swollen joints, curved spine), hearing loss and recurrent infections. (wikipedia.org)
  • Alpha-mannosidosis can impact on a patient's quality of life in many ways, including their ability to live independently, socialise or find employment. (wikipedia.org)
  • Generally, phenotypes of alpha-mannosidosis patients are not clearly distinguishable, which makes a prediction of the clinical course for an individual patient challenging. (wikipedia.org)
  • Alpha-mannosidosis is a rare inherited disorder that causes problems in many organs and tissues of the body. (medlineplus.gov)
  • Individuals with this early-onset form of alpha-mannosidosis often do not survive past childhood. (medlineplus.gov)
  • People with later-onset alpha-mannosidosis may survive into their fifties. (medlineplus.gov)
  • Alpha-mannosidosis is estimated to occur in approximately 1 in 500,000 people worldwide. (medlineplus.gov)
  • Adult alpha-mannosidosis: clinical progression in the absence of demyelination. (medlineplus.gov)
  • Used to study alpha-mannosidosis. (jax.org)
  • METHODS To study the genotype-phenotype relationship for alpha-mannosidosis 66 patients were included. (semanticscholar.org)
  • Spectrum of mutations in alpha-mannosidosis. (semanticscholar.org)