Macroglobulins: Serum globulins with high molecular weight. (Dorland, 28th ed)Serum Globulins: All blood proteins except albumin ( = SERUM ALBUMIN, which is not a globulin) and FIBRINOGEN (which is not in the serum). The serum globulins are subdivided into ALPHA-GLOBULINS; BETA-GLOBULINS; and GAMMA-GLOBULINS on the basis of their electrophoretic mobilities. (From Dorland, 28th ed)Fusobacteria: A phylum of anaerobic, gram-negative bacteria with a chemoorganotrophic heterotrophic metabolism. They are resident flora of the OROPHARYNX.Pregnancy-Associated alpha 2-Macroglobulins: Large (>160 kDa) glycoproteins with electrophoretic mobility of ALPHA-GLOBULINS, found in high concentration in the plasma of pregnant women but also in a number of species including mouse, rat, and others (ALPHA-MACROGLOBULINS). In humans, they are always present in the circulation and are nonspecific inhibitors of PROTEINASES, similar to COMPLEMENT PROTEINS (C3; C4; C5).alpha-Macroglobulins: Glycoproteins with a molecular weight of approximately 620,000 to 680,000. Precipitation by electrophoresis is in the alpha region. They include alpha 1-macroglobulins and alpha 2-macroglobulins. These proteins exhibit trypsin-, chymotrypsin-, thrombin-, and plasmin-binding activity and function as hormonal transporters.Waldenstrom Macroglobulinemia: A lymphoproliferative disorder characterized by pleomorphic B-LYMPHOCYTES including PLASMA CELLS, with increased levels of monoclonal serum IMMUNOGLOBULIN M. There is lymphoplasmacytic cells infiltration into bone marrow and often other tissues, also known as lymphoplasmacytic lymphoma. Clinical features include ANEMIA; HEMORRHAGES; and hyperviscosity.Neuraminic Acidsgamma-Globulins: Serum globulins that migrate to the gamma region (most positively charged) upon ELECTROPHORESIS. At one time, gamma-globulins came to be used as a synonym for immunoglobulins since most immunoglobulins are gamma globulins and conversely most gamma globulins are immunoglobulins. But since some immunoglobulins exhibit an alpha or beta electrophoretic mobility, that usage is in decline.Immunoelectrophoresis: A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.Immunoglobulin M: A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally being called a macroglobulin.

Structural details of proteinase entrapment by human alpha2-macroglobulin emerge from three-dimensional reconstructions of Fab labeled native, half-transformed, and transformed molecules. (1/1037)

Three-dimensional electron microscopy reconstructions of native, half-transformed, and transformed alpha2-macroglobulins (alpha2Ms) labeled with a monoclonal Fab Fab offer new insight into the mechanism of its proteinase entrapment. Each alpha2M binds four Fabs, two at either end of its dimeric protomers approximately 145 A apart. In the native structure, the epitopes are near the base of its two chisel-like features, laterally separated by 120 A, whereas in the methylamine-transformed alpha2M, the epitopes are at the base of its four arms, laterally separated by 160 A. Upon thiol ester cleavage, the chisels on the native alpha2M appear to split with a separation and rotation to give the four arm-like extensions on transformed alpha2M. Thus, the receptor binding domains previously enclosed within the chisels are exposed. The labeled structures further indicate that the two protomeric strands that constitute the native and transformed molecules are related and reside one on each side of the major axes of these structures. The half-transformed structure shows that the two Fabs at one end of the molecule have an arrangement similar to those on the native alpha2M, whereas on its transformed end, they have rotated. The rotation is associated with a partial untwisting of the strands and an enlargement of the openings to the cavity. We propose that the enlarged openings permit the entrance of the proteinase. Then cleavage of the remaining bait domains by a second proteinase occurs with its entrance into the cavity. This is followed by a retwisting of the strands to encapsulate the proteinases and expose the receptor binding domains associated with the transformed alpha2M.  (+info)

Heme and acute inflammation role in vivo of heme in the hepatic expression of positive acute-phase reactants in rats. (2/1037)

Acute-phase protein synthesis in the liver during inflammation is regulated via cytokines and glucocorticoids. Using quantitative reverse transcription (RT)-PCR analysis and immunoassay, we explored, in the rat, the response of the acute-phase protein, alpha-2 macroglobulin (A2M), after systemic inflammation induced by lipopolysaccharide (LPS) or localized inflammation induced by turpentine oil (TO). The results indicate that synthesis of A2M is higher following TO-induced inflammation than LPS-induced inflammation and is not correlated with interleukin (IL)-6 or glucocorticoid levels. We studied the putative role of heme in this differential A2M expression following localized vs. systemic inflammation; addition of heme during LPS-induced inflammation can boost the expression of A2M, whereas blocking heme synthesis (by succinyl acetone) or enhancing its consumption in parallel biosynthetic pathways (cytochrome P450 induction by phenobarbital) decreases A2M expression. This decrease was abolished by exogenous heme supplementation. Finally, we demonstrate that heme supplementation is also able to increase the A2M response in female rats to a level similar to that in male rats providing a new insight into the puzzling sexual dimorphism observed previously during localized inflammation. We propose that heme should be considered a new regulatory element in controlling liver A2M expression during inflammation.  (+info)

Pig kidney legumain: an asparaginyl endopeptidase with restricted specificity. (3/1037)

Legumain was recently discovered as a lysosomal endopeptidase in mammals [Chen, Dando, Rawlings, Brown, Young, Stevens, Hewitt, Watts and Barrett (1997) J. Biol. Chem. 272, 8090-8098], having been known previously only from plants and invertebrates. It has been shown to play a key role in processing of the C fragment of tetanus toxin for presentation by the MHC class-II system [Manoury, Hewitt, Morrice, Dando, Barrett and Watts (1998) Nature (London) 396, 695-699]. We examine here the specificity of the enzyme from pig kidney by use of protein, oligopeptide and synthetic arylamide substrates, all determinations being made at pH 5.8. In proteins, only about one in ten of the asparaginyl bonds were hydrolysed, and these were mostly predicted to be located at turns on the protein surface. Bonds that were not cleaved in tetanus toxin were cleaved when presented in oligopeptides, sometimes faster than an equivalent oligopeptide based on a bond that was cleaved in the protein. Legumain cleaved the bait region of rat alpha1-macroglobulin and was 'trapped' by the macroglobulin, as most other endopeptidases are, but did not interact with human alpha2-macroglobulin, which contains no asparagine residue in its bait region. Glycosylation of asparagine totally prevented hydrolysis by legumain. Specificity for arylamide substrates was evaluated with reference to benzyloxycarbonyl-Ala-Ala-Asn-aminomethylcoumarin, and the preference for the P3-position amino acid was Ala>Tyr(tertiary butyl)>Val>Pro>Phe=Tyr>Leu=Gly. There was no hydrolysis of substrate analogues containing mono- or di-N-methylasparagines, l-2-amino-3-ureidopropionic acid or citrulline in the P1 position. We conclude that mammalian legumain appears to be totally restricted to the hydrolysis of asparaginyl bonds in substrates of all kinds. There seem to be no strong preferences for particular amino acids in other subsites, and yet there are still unidentified factors that prevent hydrolysis of many asparaginyl bonds in proteins.  (+info)

Alpha2-macroglobulin and eosinophil cationic protein in the allergic airway mucosa in seasonal allergic rhinitis. (4/1037)

As previously demonstrated in seasonal allergic rhinitis, increased microvascular permeability and eosinophil activation are key features of allergic airway inflammation. In the present study, the hypothesis that exudation of alpha2-macroglobulin may cause the appearance of eosinophil cationic protein (ECP) in the airway lumen was explored. Nasal lavages were carried out using the nasal pool device before and during the pollen season both at baseline and after histamine challenge in 10 children with allergic rhinitis. Nasal lavage fluid levels of alpha2-macroglobulin and ECP were determined. All patients experienced nasal symptoms of allergic rhinitis during the pollen season (p<0.01-0.05). Baseline nasal lavage fluid levels of alpha2-macroglobulin and ECP were increased during the season (p<0.01-0.05) and were found to be well correlated (p<0.0001). Histamine produced concentration-dependent plasma exudation before and during the pollen season, but it was only during the pollen season that this caused an increase in the lavage fluid levels of ECP (p<0.05). These data suggest that exudation of plasma and increased tissue levels and output of eosinophil cationic protein characterize nasal mucosal inflammation in children with seasonal allergic rhinitis. The plasma exudation process in part may account for lumenal entry of eosinophil cationic protein molecules that have been released in mucosal tissue compartments. A combination of induced exudation and nasal lavage may improve the yield of important markers of inflammation in studies of nasal diseases.  (+info)

NMR solution structure of complement-like repeat CR8 from the low density lipoprotein receptor-related protein. (5/1037)

The low density lipoprotein receptor-related protein is a member of the low density lipoprotein receptor family and contains clusters of cysteine-rich complement-like repeats of about 42 residues that are present in all members of this family of receptors. These clusters are thought to be the principal binding sites for protein ligands. We have expressed one complement-like repeat, CR8, from the cluster in lipoprotein receptor-related protein that binds certain proteinase inhibitor-proteinase complexes and used three-dimensional NMR on the 13C/15N-labeled protein to determine the structure in solution of the calcium-bound form. The structure is very similar in overall fold to repeat 5 from the low density lipoprotein receptor (LB5), with backbone root mean square deviation of 1.5 A. The calcium-binding site also appears to be homologous, with four carboxyl and two backbone carbonyl ligands. However, differences in primary structure are such that equivalent surfaces that might represent the binding interfaces are very different from one another, indicating that different domains will have very different ligand specificities.  (+info)

Abnormality in urinary protein excretion in Japanese men with impaired glucose tolerance. (6/1037)

OBJECTIVE: To examine whether subjects with impaired glucose tolerance (IGT) for more than 2 years have any abnormality in the kidney. RESEARCH DESIGN AND METHODS: We measured urinary excretion rate and clearance of various plasma proteins with different molecular radii and different isoelectric points in 22 Japanese men with IGT (IGT group) and 37 age-matched healthy control subjects (control group). RESULTS: Clearance of ceruloplasmin (molecular radius approximately 45 A; isoelectric point 4.4), IgG4 (molecular radius 55 A; isoelectric point 5.4), and IgG (molecular radius 55 A; isoelectric point 7.4) was significantly higher in the IGT group than in the control group, whereas there were no significant differences in urinary excretion rate of albumin (molecular radius 36 A; isoelectric points 4.8-5.2) and clearance of alpha 2-macroglobulin (molecular radius 88 A; isoelectric point 5.4) between the two groups. CONCLUSIONS: In the present study, we found that clearance of neutral-charged IgG, negatively charged IgG4, and ceruloplasmin with molecular radii of approximately 45-55 A was selectively increased in IGT subjects. This finding does not seem to be explained by impairment of charge and pore-size selectivity in the glomerulus. Therefore, considering the present result together with our recent finding that enhanced glomerular filtration rate (GFR) after acute protein loading in healthy subjects induced a selective increase in clearance of IgG, IgG4, and ceruloplasmin, we suggest that increased intraglomerular hydraulic pressure, although enhanced GFR was not demonstrated, may be at work in these mildly hyperglycemic subjects.  (+info)

Protein GRAB of streptococcus pyogenes regulates proteolysis at the bacterial surface by binding alpha2-macroglobulin. (7/1037)

In the molecular interplay between pathogenic microorganisms and their host, proteolytic mechanisms are believed to play a crucial role. Here we find that the important human pathogen Streptococcus pyogenes (group A Streptococcus) expresses a surface protein with high affinity (Ka = 2.0 x 10(8) M-1) for alpha2-macroglobulin (alpha2M), the dominating proteinase inhibitor of human plasma. The immunoglobulin-binding protein G of group C and G streptococci also contains an alpha2M-binding domain and a gene encoding protein GRAB (protein G-related alpha2M-binding protein) was identified in the S. pyogenes Genome Sequencing data base. The grab gene is present in most S. pyogenes strains and is well conserved. Protein GRAB has typical features of a surface-attached protein of Gram-positive bacteria. It also contains a region homologous to parts of the alpha2M-binding domain of protein G and a variable number of a unique 28-amino acid-long repeat. Using Escherichia coli-produced protein GRAB and synthetic GRAB peptides, the alpha2M-binding region was mapped to the NH2-terminal part of protein GRAB, which is the region with homology to protein G. An isogenic S. pyogenes mutant lacking surface-associated protein GRAB showed no alpha2M binding activity and was attenuated in virulence when injected intraperitoneally in mice. Finally, alpha2M bound to the bacterial surface via protein GRAB was found to entrap and inhibit the activity of both S. pyogenes and host proteinases, thereby protecting important virulence determinants from proteolytic degradation. This regulation of proteolytic activity at the bacterial surface should affect the host-microbe relation during S. pyogenes infections.  (+info)

Destruction of articular cartilage by alpha 2 macroglobulin elastase complexes: role in rheumatoid arthritis. (8/1037)

OBJECTIVE: Neutrophil elastase accounts for the ability of some fresh rheumatoid synovial fluids to degrade cartilage matrix in vitro. The aim of this study was to determine if enzyme activity could result from depletion of synovial fluid inhibitors or protection of the enzyme from inhibition. METHODS: The ability of synovial fluids to inhibit porcine pancreatic elastase was investigated together with chemical pretreatments capable of inactivating alpha 1 protease inhibitor (alpha 1PI) or preventing formation of alpha 2 macroglobulin (alpha 2M) elastase complexes. Subsequently, complexes of human neutrophil elastase with alpha 2M were prepared and applied to frozen sections of cartilage. Proteoglycan loss was quantified by alcian blue staining and scanning and integrating microdensitometry. Parallel studies were carried out using a low molecular weight chromogenic elastase substrate. The effects of alpha 1PI and SF on these systems were investigated. Finally, synovial fluids were subjected to gel filtration and the fractions assayed for elastase activity. High molecular weight fractions were pooled, concentrated, and tested for their ability to degrade cartilage sections. RESULTS: All synovial fluids reduced the activity of porcine pancreatic elastase, the inhibition mainly being attributable to alpha 1PI, whereas remaining activity resulted from complexes of elastase with alpha 2M. Complexes of human neutrophil elastase with alpha 2M were shown to cause proteoglycan degradation in frozen sections of human articular cartilage. Alpha 1PI prevented alpha 2M elastase complexes from degrading cartilage but not the chromogenic substrate. The data suggested that alpha 1PI does not inhibit elastase bound to alpha 2M but sterically hinders the complex. However, only one of five synovial fluids was able to completely block the actions of alpha 2M elastase complexes against cartilage. Gel filtration of rheumatoid synovial fluids showed elastase and cartilage degrading activity to be associated with fractions that contained alpha 2M, and not with fractions expected to contain free enzyme. CONCLUSIONS: The data suggest that synovial fluid alpha 2M elastase complexes can degrade cartilage matrix in rheumatoid arthritis.  (+info)

*Alpha-2-Macroglobulin

The loss of alpha-2-macroglobulin into urine is prevented by its large size. The net result is that alpha-2-macroglobulin ... Overall, alpha-2-Macroglobulin is the largest major nonimmunoglobulin protein in human plasma. The alpha-macroglobulin (aM) ... 10-15% of copper in human plasma is chelated by alpha-2-macroglobulin. alpha-2-Macroglobulin levels are increased when the ... of alpha-2-macroglobulin leads to increased risk of Alzheimer's disease. alpha-2-Macroglobulin binds to and removes the active ...

*Pregnancy zone protein

1989). "A cluster of alpha 2-macroglobulin-related genes (alpha 2 M) on human chromosome 12p: cloning of the pregnancy-zone ... "Proteolytic hydrolysis and purification of the LRP/alfa-2-macroglobulin receptor domain from alpha-macroglobulins". Protein ... Comparison with human alpha 2-macroglobulin". J. Biol. Chem. 260 (29): 15723-35. PMID 2415522. Sottrup-Jensen L, Birkedal- ... Genomic structure of the bait domains of human pregnancy zone protein and alpha 2 macroglobulin". FEBS Lett. 262 (2): 349-52. ...

*A2ML1

Alpha-2-macroglobulin-like 1 abbreviated as α2ML1 is a protein that in humans is encoded by the A2ML1 gene. α2ML1 is a large, ... This gene encodes a member of the alpha-macroglobulin superfamily. The encoded protein acts as an inhibitor for several ... Alpha-2-macroglobulin-like 1". Galliano MF, Toulza E, Gallinaro H, Jonca N, Ishida-Yamamoto A, Serre G, Guerrin M (November ... "The protease inhibitor alpha-2-macroglobulin-like-1 is the p170 antigen recognized by paraneoplastic pemphigus autoantibodies ...

*Interstitial collagenase

Cleaves synthetic substrates and alpha-macroglobulins at bonds where P1' is a hydrophobic residue This enzyme belongs to the ... Localization of cleavage sites in the bait regions of five mammalian α-macroglobulins". J. Biol. Chem. 264: 393-401. PMID ... Sottrup-Jensen, L.; Birkedal-Hansen, H. (1989). "Human fibroblast collagenase-α-macroglobulin interactions. ...

*Fibrinolysis

Alpha 2-antiplasmin and alpha 2-macroglobulin inactivate plasmin. Plasmin activity is also reduced by thrombin-activatable ... Such results can be seen in peoples with liver disease, PAI-1 deficiency or alpha 2-antiplasmin deficiency. Similar results are ... Nevertheless, excess levels of PAI and alpha 2-antiplasmin have been implicated in the metabolic syndrome and various other ... alpha 2-antiplasmin, and plasminogen) from plasma and then observing the time required for clot dissolution. A shortened lysis ...

*LDL-receptor-related protein-associated protein

It is a glycoprotein that binds to the alpha-2-macroglobulin receptor, as well as to other members of the low density ... Jou YS, Goold RD, Myers RM (Nov 1994). "Localization of the alpha 2-macroglobulin receptor-associated protein 1 gene (LRPAP1) ... coding for the alpha 2-macroglobulin receptor-associated protein and structural comparison with the murine gene coding for the ... "Primary structure of alpha 2-macroglobulin receptor-associated protein. Human homologue of a Heymann nephritis antigen". The ...

*TGF beta 2

Comparison to the TGF-beta-alpha 2-macroglobulin interaction". Eur. J. Biochem. 221 (2): 687-93. doi:10.1111/j.1432-1033.1994. ...

*Acute-phase protein

Alpha 2-macroglobulin and coagulation factors affect coagulation, mainly stimulating it. This pro-coagulant effect may limit ... 1993). "Alpha-2-macroglobulin functions as an inhibitor of fibrinolytic, clotting, and neutrophilic proteinases in sepsis: ...

*Developmental Haemostasis

alpha-2-Macroglobulin (A2M): is also a major inhibitor of thrombin. It is present in 2-3 fold higher concentration during ... the functional role of alpha-2-macroglobulin and influence of Developmental Haemostasis. British Journal of Haematology, 138(3 ...

*Interleukin-6 receptor

"Gene expression of the high molecular weight proteinase inhibitor alpha 2-macroglobulin". Biol. Chem. Hoppe-Seyler. 373 (7): ... Kluck PM, Wiegant J, Jansen RP, Bolk MW, Raap AK, Willemze R, Landegent JE (1993). "The human interleukin-6 receptor alpha ... The interleukin-6 receptor can serve as an alpha-receptor for CTNF". J. Biol. Chem. 278 (11): 9528-35. doi:10.1074/jbc. ...

*List of MeSH codes (D12.776)

... alpha 1-antichymotrypsin MeSH D12.776.377.715.085.085 - alpha 1-antitrypsin MeSH D12.776.377.715.085.100 - alpha-macroglobulins ... alpha-macroglobulins MeSH D12.776.402.150.100.200 - chimerin 1 MeSH D12.776.402.150.500.460 - neurofibromin 1 MeSH D12.776. ... alpha-crystallin a chain MeSH D12.776.306.366.100.300 - alpha-crystallin b chain MeSH D12.776.306.366.300.100 - beta-crystallin ... steroid 12-alpha-hydroxylase MeSH D12.776.422.220.453.915.737 - steroid 16-alpha-hydroxylase MeSH D12.776.422.220.453.915.748 ...

*List of MeSH codes (D12.776.124)

... alpha-macroglobulins MeSH D12.776.124.790.720.100.500 -- pregnancy-associated alpha 2-macroglobulins. ... alpha 1-antitrypsin MeSH D12.776.124.790.106.100 -- alpha-macroglobulins MeSH D12.776.124.790.106.118 -- antiplasmin MeSH ... immunoglobulin alpha-chains MeSH D12.776.124.486.485.114.619.251 -- immunoglobulin d MeSH D12.776.124.486.485.114.619.251.500 ... immunoglobulin alpha-chains MeSH D12.776.124.486.485.705.500.360 -- immunoglobulin delta-chains MeSH D12.776.124.486.485.705. ...

*Placental alpha microglobulin-1 (PAMG-1)

Placental alpha macroglobulin-1 (PAMG-1) has been the subject of over 20 clinical investigations, the majority of which have ... Placental alpha microglobulin-1 (PAMG-1) is a human protein that was first isolated in 1975 from amniotic fluid. PAMG-1 is an ... PAMG-1 was originally referred to as specific alpha-1 globulin of placenta. PAMG-1 is present in blood and the amniotic fluid ... 2012 Sep;25(9):1690-8. Sukchaya K, Phupong V. A comparative study of positive rate of placental alpha-microglobulin-1 test in ...

*Low-density lipoprotein receptor-related protein 8

ApoER2 splice variants can act as a receptor for alpha-2-macroglobulin which can have a role in clearance of alpha-2- ... which is controlled by proteolytic inhibitors such as alpha-2-macroglobulin. Studies have shown that a high presence of alpha-2 ... Isolation of cDNA encoding proteins associated with Aβ was used to discover alpha-2-macroglobulin. These discoveries may link ... alpha-2-macroglobulin and its receptors, one of them being ApoER2, to Alzheimer's disease. ApoER2 interaction with reelin and ...

*Glucocorticoid receptor

... synergy with GR for full transcriptional increase of the alpha 2-macroglobulin gene". Genes Dev. 17 (20): 2564-77. doi:10.1101/ ... The role of alpha and beta isoforms of the glucocorticoid receptor]". Arch. Bronconeumol. 38 (9): 436-40. PMID 12237016. Bray ...

*STAT3

... synergy with GR for full transcriptional increase of the alpha 2-macroglobulin gene". Genes & Development. 17 (20): 2564-77. ... Kawasaki A, Matsumura I, Kataoka Y, Takigawa E, Nakajima K, Kanakura Y (May 2003). "Opposing effects of PML and PML/RAR alpha ... "Involvement of tyrosine phosphatase PTP1D in the inhibition of interleukin-6-induced Stat3 signaling by alpha-thrombin". ...

*LRP1

Low density lipoprotein receptor-related protein 1 (LRP1), also known as alpha-2-macroglobulin receptor (A2MR), apolipoprotein ... "The low density lipoprotein receptor-related protein/alpha 2-macroglobulin receptor binds and mediates catabolism of bovine ... The cytoplasmic domain of LRP1 is the alpha-chain, which comprises four ligand-binding domains (numbered I-IV) containing two, ... LRP1 has been shown to interact with: A2-Macroglobulin, β-amyloid precursor protein, APBB1, APOE, Aprotinin, C1S/C1q inhibitor ...

*Lipoprotein lipase

"The low density lipoprotein receptor-related protein/alpha 2-macroglobulin receptor binds and mediates catabolism of bovine ... domain of lipoprotein lipase binds to the low density lipoprotein receptor-related protein/alpha 2-macroglobulin receptor (LRP ...

*Withaferin A

Anti-inflammatory property of withaferin A has been attributed to its ability to suppress alpha-2 macroglobulin, NF-κB and AP1 ... It binds to intermediate filament protein, vimentin by covalently modifying its highly conserved cysteine residue in alpha- ...

*KLK7

... elafin and alpha-2-Macroglobulin-like 1. Both Zn2+ and Cu2+ ions are also able to inhibit KLK7. KLK7 is a chymotrypsin-like ... "A novel protease inhibitor of the alpha2-macroglobulin family expressed in the human epidermis". J Biol Chem. 281 (9): 5780- ...

*José Antonio López Guerrero

Interaction of alpha-2-Macroglobulin and HSV-1 during infection of neuronal cells». Journal of Neurovirology., 7: 556-63. Bello ... connection with the tumor necrosis factor alpha signalling pathway». Journal of Virology, 72: 8893-8903. López-Guerrero, J. A ...

*Nephrotic syndrome

... such as alpha-2 macroglobulin and lipoproteins. An increase in the latter can cause the hyperlipidemia associated with this ...

*Alpha-1-microglobulin/bikunin precursor

Identification of a complex with alpha 1-inhibitor-3, a rat alpha 2-macroglobulin homologue". J. Biol. Chem. 265 (27): 16150-7 ... Alpha-1-microglobulin/bikunin precursor has been shown to interact with CD79A. Inter-alpha-trypsin inhibitor Alpha-1- ... "The mRNA for a proteinase inhibitor related to the HI-30 domain of inter-alpha-trypsin inhibitor also encodes alpha-1- ... "Homologous chromosomal locations of the four genes for inter-alpha-inhibitor and pre-alpha-inhibitor family in human and mouse ...

*POTEB

... and alpha-2-macroglobulin. This data is based on affinity capture- mass spectrometry. POTEB expression is low or completely ... However, there is unpublished data suggesting an interaction between POTEB and alpha-1-B glycoproteins, APOBEC1 complementation ...

*Pancreatic elastase

Alpha 1-antitrypsin and alpha-2-macroglobulin are human serum protease inhibitors that completely inhibit the general ...

*Index of biochemistry articles

... alpha-beta T-cell antigen receptor - alpha-fetoprotein - alpha-globulin - alpha-macroglobulin - alpha-MSH - Ames test - amide ... alpha adrenergic receptor - Alpha helix - alpha-1 adrenergic receptor - alpha-2 adrenergic receptor - ... transforming growth factor alpha - transforming growth factor beta - transforming growth factor beta receptor - transient ... interferon-alpha - interferon-beta - interleukin receptor - interleukin-1 receptor - interleukin-2 receptor - interleukin-3 - ...
Rat alpha 1-macroglobulin (alpha 1M), rat alpha 2-macroglobulin (alpha 2M) migrated as single bands on non-denaturing gels when purified by the methods described. All three proteins demonstrated increased mobility after reaction with trypsin. A single saturable pathway rapidly cleared complexes of trypsin and the alpha-macroglobulins of mouse, rat and human from the circulation of mice. None of the native alpha-macroglobulins competed for clearance with the trypsin complexes. [14C]Methylamine incorporation was 4.1, 3.9, 2.6 and 3.2 mol/mol of proteinase inhibitor for human alpha 2M, rat alpha 1M, rat alpha 2M and mouse alpha 2M, respectively. Only rat alpha 2M, the acute-phase alpha-macroglobulin studied, showed no evidence of conformational change when subjected to electrophoresis after reaction with methylamine. The clearance of rat alpha 2M-methylamine was comparable with that of the native molecule. The other alpha-macroglobulin-methylamine complexes cleared faster than the inhibitors that ...
Is able to inhibit all four classes of proteinases by a unique trapping mechanism. This protein has a peptide stretch, called the bait region which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase ...
The binding of 125I-labelled transforming growth factor-beta 1 (TGF-beta 1) to human alpha 2-macroglobulin (alpha 2M) was studied by native PAGE and autoradiography. TGF-beta 1 bound preferentially to alpha 2M-methylamine and minimally, if at all, to native alpha 2M. Preparations of alpha 2M-proteinase complex were generated by incubating a standard concentration of alpha 2M (0.4 microM) with different concentrations of trypsin, chymotrypsin or neutrophil elastase (0.04-2.0 microM). The 125I-TGF-beta 1-binding activity depended on the initial ratio of active proteinase to alpha 2M, or r value, used to form the alpha 2M-proteinase complex. With all three proteinases, r values of 2 or greater yielded preparations with unchanged or decreased TGF-beta 1-binding activity relative to native alpha 2M. By contrast, r values near 1 yielded preparations with significantly increased TGF-beta 1-binding activity. The results of [3H]thymidine-incorporation studies performed in mouse keratinocytes were ...
How is Alpha 2 Macroglobulin Receptor abbreviated? A2MR stands for Alpha 2 Macroglobulin Receptor. A2MR is defined as Alpha 2 Macroglobulin Receptor somewhat frequently.
Extract: The present study using immunologic methodology confirms previous observations from this laboratory of an absence of a protease component with arginine esterase activity in plasma of patients with cystic fibrosis. In this study, the pooled plasma from control individuals was activated and partially purified after adsorption on columns of soybean trypsin inhibitor conjugated to Sepharose 4B followed by elution with benzamidine. The fraction was further purified by isoelectrofocusing on polyacrylamide gels. Proteins around the pI range of 5.5 were eluted and utilized to prepare an antiserum. Immunoelectrophoresis of activated plasma samples from control subjects and patients with cystic fibrosis was performed utilizing the antiserum. In controls, four precipitin arcs with residual esterase activity were observed, whereas only three were seen in plasma from patients with cystic fibrosis. Double gel diffusion experiments using specific antisera ruled out the presence of trypsin, chymotrypsin,
Recent reports have suggested that variability in the alpha2-macroglobulin gene is a genetic risk factor for Alzheimers disease. Here we have both tested a common polymorphism in the gene (I1000V) for association with the disease in a four-site case control study design, and tested the locus for linkage in a large series of sibpairs afflicted with late onset disease. Our results fail to show an association between this polymorphism and disease.
Recent reports have suggested that variability in the alpha2-macroglobulin gene is a genetic risk factor for Alzheimers disease. Here we have both tested a common polymorphism in the gene (I1000V) for association with the disease in a four-site case control study design, and tested the locus for linkage in a large series of sibpairs afflicted with late onset disease. Our results fail to show an association between this polymorphism and disease.
Is able to inhibit all four classes of proteinases by a unique trapping mechanism. This protein has a peptide stretch, called the bait region which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase (By similarity). Displays inhibitory activity against chymotrypsin, papain, thermolysin, subtilisin A and, to a lesser extent, elastase but not trypsin. May play an important role during desquamation by inhibiting extracellular proteases ...
Twenty-five monoclonal antibodies were generated by immunizing mice. Of these, the one named R10 had the strongest neutralizing activity against HAV [a 50% neutralizing concentration (neut50) value of ∼2 nM, ∼0.3 μg/mL]. The Fab fragment is also strongly neutralizing (neut50 = ∼3 nM, ∼0.45 μg/mL) (Fig. 1A). R10 does not recognize linear epitopes by immunoblot, but recognizes conformational epitopes by ELISA, and shows similar binding affinities to full and empty particles (Fig. 1B). Interestingly, a fluorescent assay revealed that whereas the R10 Fab portion destabilizes full HAV particles by 7 °C, the intact R10 stabilizes the particles by 2 °C (Fig. 1C and Fig. S1A). The same assay indicates that the bivalent intact antibody induces a two-stage transition in protein conformation to release the RNA genome (Fig. 1C and Fig. S1A). The first event, producing a slight exposure of RNA, occurs at a similar temperature to that seen with the Fab, whereas the second, which fully exposes the ...
This is a domain of the alpha-2-macroglobulin family.. The alpha-macroglobulin (aM) family of proteins includes protease inhibitors [(PUBMED:2473064)], typified by the human tetrameric a2-macroglobulin (a2M); they belong to the MEROPS proteinase inhibitor family I39, clan IL. These protease inhibitors share several defining properties, which include (i) the ability to inhibit proteases from all catalytic classes, (ii) the presence of a bait region and a thiol ester, (iii) a similar protease inhibitory mechanism and (iv) the inactivation of the inhibitory capacity by reaction of the thiol ester with small primary amines. aM protease inhibitors inhibit by steric hindrance [(PUBMED:2472396)]. The mechanism involves protease cleavage of the bait region, a segment of the aM that is particularly susceptible to proteolytic cleavage, which initiates a conformational change such that the aM collapses about the protease. In the resulting aM-protease complex, the active site of the protease is sterically ...
α2M was the most notable protein to appear in abundance shortly before the time of implantation. It is closely related to pregnancy zone protein (PZP) and three members of the complement system [23]. It is the largest non-immunoglobulin protein in circulation in mammalian blood, comprises an approximately 160 kDa polypeptide that is glycosylated, and usually occurs in circulation as a tetramer comprising a pair of non-covalently associated disulfide-linked dimers [23]. It is synthesized mainly in the liver, but also in the decidua and endometrium in mice and possibly also in humans, and is thought to be important for implantation [12,24-27].. α2M is a minor acute-phase inflammation reactant in several species [28]. It operates via two mechanisms as a broad-spectrum proteinase inhibitor [23]. In one mechanism, an active proteinase cleaves a bait peptide that causes α2M to fold around and encapsulate the target enzyme. In the other, the target proteinase is covalently bound via a thioester ...
pep:known chromosome:VEGA66:6:128483567:128526720:-1 gene:OTTMUSG00000022212 transcript:OTTMUST00000052790 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Pzp description:pregnancy zone protein ...
Looking for Alpha-2 macroglobulin? Find out information about Alpha-2 macroglobulin. Any gamma globulin with a sedimentation constant of 195 Explanation of Alpha-2 macroglobulin
The present invention relates to the use of alpha (2) macroglobulin complexes isolated from the serum of a mammal. The invention also relates to methods for making such complexes and compositions com
alpha 2 Macroglobulin antibody [N2N3] (alpha-2-macroglobulin) for WB. Anti-alpha 2 Macroglobulin pAb (GTX112856) is tested in Human samples. 100% Ab-Assurance.
Now you can calculate your Pregnancy Fertility by using the Fertility calculator and here are the steps to calculate fertility by using a calculator.
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Pregnant women beware - if you eat genetically modified organism or the GMO foods, you may be exposing your unborn baby to dangerous toxins.
BioAssay record AID 4853 submitted by ChEMBL: Compound was tested for its ability to displace [125I]GTI binding to 5-hydroxytryptamine 1D receptor recognition sites in pig caudate membranes.
Described in this unit are ligand‐binding assays for GABAA, GABAB, and the homomeric ρ GABAA (formerly GABAC) receptor recognition sites in brain tissue
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Buy our Natural Human alpha 2 Macroglobulin protein. Ab91104 is a full length protein produced in Nativesyntheticaly and has been validated in SDS-PAGE. Abcam…
Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. [provided by RefSeq, Jul 2008]
(= a 2 macroglobulin) Large (725 kD) plasma antiprotease with very broad spectrum of inhibitory activity against all classes of proteases. Apparently works by trapping the protease within a cage that closes when the protease sensitive bait…
ウサギ・ポリクローナル抗体 ab58703 交差種: Rat,Hu 適用: WB,ELISA,IHC-P,ICC…alpha 2 Macroglobulin抗体一覧…画像、プロトコール、文献などWeb上の情報が満載のアブカムの Antibody…
マウス・ポリクローナル抗体 ab52651 交差種: Ms 適用: WB…alpha 2 Macroglobulin抗体一覧…画像、プロトコール、文献などWeb上の情報が満載のアブカムの Antibody 製品。国内在庫と品質保証制度も充実。
Cytonics Corporation is developing therapeutics based on the protease inhibitor alpha-2 macroglobulin (A2M) for the treatment of post-traumatic osteoarthritis
Alpha 2 Macroglobulin is a large plasma protein found in the blood. It is produced by the liver, and is a major component of the alpha-2 band in protein
Provided are compounds of Formula I: Formula I or a pharmaceutically acceptable salt thereof, wherein R 2 , R 4 , R 5 , R 6 , R 7 , R 8 , R 9 , X, Z 1 , Z 2 , Z 3 , Z 4 and m are as defined herein. Also provided is a pharmaceutically acceptable composition comprising a compound of Formula I, or a pharmaceutically acceptable salt thereof. Also provided are methods of using a compound of Formula I, or a pharmaceutically acceptable salt thereof.
cdna chromosome:GRCm38:6:121636228:121679227:1 gene:ENSMUSG00000030111 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:A2m description:alpha-2-macroglobulin [Source:MGI Symbol;Acc:MGI:2449119 ...
Please optimize the BrU concentraiton, pulse-time, or chase-time. BrU concentrations of more than 500 μM may affect cell growth and/or other biological processes ...
GoPubMed lists recent and important papers and reviews for low density lipoprotein receptor-related protein 12(Suppression of tumorigenicity)
Hep I stimulates association of LRP with the Gαi2 subunit, and association is blocked by RAP and by peptides corresponding to the COOH terminus of Gαi2. (A) BAE cells were grown to near confluence and serum deprived overnight. Cells were then treated with 1 μM hep I for 0, 5, 10, 15, or 30 min and lysed. Cell lysates were immunoprecipitated with monoclonal anti-LRP antibody (8G1), separated by SDS-PAGE, and immunoblotted with mouse anti-Gαi2 antibodies. A representative immunoblot for LRP-associated Gαi2 is shown. Bands were analyzed using One-Dscan software (Scanalytics), and the fold change in LRP-Gαi2 association as compared with untreated conditions was determined. Equal loading of sample protein was assessed by protein staining with Ponceau S. *, P , 0.05; **, P , 0.01 (n = 3-5). (B) BAE cells were grown as in A, treated for 10 min with either DMEM, 100 nM hep I, 78 nM TSP, or 100 nM modified hep I and assayed for LRP-Gαi2 association as described in A. A representative immunoblot ...
Disease-causing bacteria in the lung evade the bodys immune system by pretending the lungs are pregnant, according to University of Dundee research presented today at the European Respiratory Society Congress in Madrid.. A new study, funded by the British Lung Foundation and the Scottish Governments Chief Scientist Office, has revealed that people with two common lung diseases - COPD and bronchiectasis - produce Pregnancy Zone Protein (PZP) in their lungs when they have a chest infection.. For the full article, please see here:. https://www.dundee.ac.uk/news/2019/lung-bacteria-feign-pregnancy-to-avoid-detection.php. ...
1D2L: NMR solution structure of complement-like repeat CR3 from the low density lipoprotein receptor-related protein. Evidence for specific binding to the receptor binding domain of human alpha(2)-macroglobulin.
phdthesis{e0687830-50ba-47d3-97d5-1bd517693461, abstract = {Cell wall-attached proteins (CWPs) are important for the virulence of Gram-positive bacteria, but are also targets for the host immune system. CWPs have a conserved COOH-terminal region, responsible for cell wall-sorting. A pattern based on this conserved region was developed. The pattern identifies genes encoding CWPs, with high sensitivity and specificity, in whole genomes of Gram-positive bacteria. In genomes of five Gram-positive bacterial species, 19 previously unknown putative CWPs were identified.,br/,,br, ,br/,,br, Three novel CWPs were identified and characterised in <i>Streptococcus pyogenes</i>, an important Gram-positive human pathogen. One CWP, GRAB, binds the human proteinase inhibitor alpha<sub>2</sub>-macroglobulin to the <i>S. pyogenes</i> surface, thus protecting other CWPs from proteolysis. Moreover, two novel CWPs (SclA and SclB) with regions similar to collagen were studied. These ...
Lrp5 - Lrp5 (untagged) - Mouse low density lipoprotein receptor-related protein 5 (Lrp5), (10ug) available for purchase from OriGene - Your Gene Company.
Ulery PG, Beers J, Mikhailenko I, Tanzi RE, Rebeck GW, Hyman BT, Strickland DK. Modulation of beta-amyloid precursor protein processing by the low density lipoprotein receptor-related protein (LRP). Evidence that LRP contributes to the pathogenesis of Alzheimers disease ...
Using cryo-electron microscopy, an international group of scientists have reported a potent HAV-specific neutralizing monoclonal antibody R10 that blocks receptor attachment and interferes viral uncoating. High resolution cryo-EM structures of HAV full and empty particles and of the complex of HAV with R10 Fab reveal the atomic details of antibody binding and point to a receptor recognition site at the pentamer interface. HAV is unique amongst picornaviruses in targeting the liver, and despite a successful vaccine continues to be a source of mortality. It has radically different properties from other picornaviruses, for instance it exists in an enveloped form and is unusually stable, both genetically and physically. In 2015, IBP scientists solved crystal structure of unenveloped HAV showing no trace of the canyon which encircles the 5-fold axes of enteroviruses and is often the site of receptor binding. However, the molecular basis where the cellular receptor TIM-1 might attach remains unknown. ...
Glutamatergic and monoaminergic networks in corticolimbic structures and the basal ganglia play an important role in the control of motor function. Correspondingly, an understanding of the mechanisms via which they exert their actions may lead to novel therapies for the improved management of neurological disorders, such as Parkinsons disease, and psychiatric diseases, such as schizophrenia (Carlsson and Carlsson, 1990; Lange et al., 1997; Schmidt and Kretschmer, 1997). To this end, it is important to identify functional models that allow the exploration of interrelationships among glutamatergic and monoaminergic pathways. In previous studies, we demonstrated that the interruption of transmission at N-methyl-d-aspartate (NMDA) receptors by open channel blockers, such as dizocilpine, and antagonists of the NMDA receptor recognition site, such as 3-(2-carboxypiperazin-4-yl)-propyl-1-phosphonic acid (CPP), but not by glycineB antagonists, such as L701,324, elicits STFs in rats (Millan et al., ...
You need to find all english words ending in M ? We have that list and for sure you will find the word you are looking for. All these words ending in M are sorted by the number of points they give at Scrabble.
Fórum. Old Punx fest - letní punkový open air festival se zcela novou a originální koncepcí. Vystoupí zde pouze legendy - kapely, které jsou na scéně déle než 18 let. To vše v novém areálu Milín..
Fórum. Old Punx fest - letní punkový open air festival se zcela novou a originální koncepcí. Vystoupí zde pouze legendy - kapely, které jsou na scéně déle než 18 let. To vše v novém areálu Milín..
where n and m are just integers. These kind of integrals come up all the time in vector calculus and they take me ages to do. Is there a general method of doing them or a few common integrals i could learn? I end up having to apply about 3 trig identites and then sorting out the mess of resulting sinxs, cosx, cos2x ...
A multibranched polyoxyalkylene compound represented by the following formula (1): ##STR00001## wherein R.sup.1 is the same or different and is a hydrocarbon group having 1 to 24 carbon atoms, OA.sup.1 and OA.sup.2 are the same or different and are an oxyalkylene group having 2 to 4 carbon atoms, n and m are the same or different and are an average ...
Natures Aid Serrapeptase is an immunologically active enzyme. It can bind itself to the alpha 2 macroglobulin in our plasma where it is shielded from the immune system while retaining its enzymatic activity.
In the measurement of plasmin inhibitor three reactions of plasmin are of importance. First is the reaction with the PB-form of the plasmin inhibitor. Second is the reaction with the NPB-form of the plasmin inhibitor and third the reaction with a 2 -macroglobulin. Using a small excess of plasmin ( approx. 1 m M), all three reactions take place as a second order reaction ([plasmin inhibitor] total approx. [plasmin] total ). At a plasmin inhibitor concentration of 1 m M gives this a velocity for the plasmin-PB-plasmin inhibitor complex formation of 27 s -1 . For the plasmin-NPB-plasmin inhibitor complex formation this is 0.2 s -1 and for the plasmin- a 2 -macroglobulin complex formation this is 1.0 s -1 . Expressed in ratios of the complexes this is 95.7% for the plasmin-PB-plasmin inhibitor complex, 0.7% for the plasmin-NPB-plasmin inhibitor complex and 3.6% for the plasmin- a 2 -macroglobulin complex ...
Gelatinase B/matrix metalloproteinase-9 (MMP-9), a key regulator and effector of immunity, contains a C-terminal hemopexin domain preceded by a unique linker sequence of ?64 amino acid residues. This linker sequence is demonstrated to be an extensively O-glycosylated (OG) domain with a compact three-dimensional structure. The OG and hemopexin domains have no influence on the cleavage efficiency of MMP-9 substrates. In contrast, the hemopexin domain contains a binding site for the cargo receptor low density lipoprotein receptor-related protein-1 (LRP-1). Furthermore, megalin/LRP-2 is identified as a new functional receptor for the hemopexin domain of MMP-9, able to mediate the endocytosis and catabolism of the enzyme. The OG domain is required to correctly orient the hemopexin domain for inhibition by TIMP-1 and internalization by LRP-1 and megalin. Therefore, the OG and hemopexin domains down-regulate the bioavailability of active MMP-9 and the interactions with the cargo receptors are proposed ...
LRP1 - LRP1 (Myc-DDK-tagged)-Human low density lipoprotein receptor-related protein 1 (LRP1) available for purchase from OriGene - Your Gene Company.
Inhibition of AB5-type bacterial toxins can be achieved by heterobifunctional ligands (BAITs) that mediate assembly of supramolecular complexes involving the toxins pentameric cell membrane-binding subunit and an endogenous protein, serum amyloid P component, of the innate immune system. Effective in vivo protection from Shiga toxin Type 1 (Stx1) is achieved by polymer-bound, heterobifunctional inhibitors-adaptors (PolyBAITs), which exhibit prolonged half-life in circulation and by mediating formation of face-to-face SAP-AB5 complexes, block receptor recognition sites and redirect toxins to the spleen and liver for degradation. Direct correlation between solid-phase activity and protective dose of PolyBAITs both in the cytotoxicity assay and in vivo indicate that the mechanism of protection from intoxication is inhibition of toxin binding to the host cell membrane. The polymeric scaffold influences the activity not only by clustering active binding fragments but also by sterically interfering with the
Inhibition of AB5-type bacterial toxins can be achieved by heterobifunctional ligands (BAITs) that mediate assembly of supramolecular complexes involving the toxins pentameric cell membrane-binding subunit and an endogenous protein, serum amyloid P component, of the innate immune system. Effective in vivo protection from Shiga toxin Type 1 (Stx1) is achieved by polymer-bound, heterobifunctional inhibitors-adaptors (PolyBAITs), which exhibit prolonged half-life in circulation and by mediating formation of face-to-face SAP-AB5 complexes, block receptor recognition sites and redirect toxins to the spleen and liver for degradation. Direct correlation between solid-phase activity and protective dose of PolyBAITs both in the cytotoxicity assay and in vivo indicate that the mechanism of protection from intoxication is inhibition of toxin binding to the host cell membrane. The polymeric scaffold influences the activity not only by clustering active binding fragments but also by sterically interfering with the
Fibrinogen γ-chain dodecapeptide, 5 mg. The synthetic dodecapeptide HHLGGAKQAGDV represents the specific platelet receptor recognition site of the human fibrinogen �� chain (residues 400-411).
Abstract. Intrahepatic transplantation of islets requires a lot of islets because more than 50% of the graft is lost during the 24 hours following transplantation. We analyzed, in a rat model, early post-transplantation inflammation using systemic inflammatory markers, or directly in islet-transplanted livers by immunohistochemistry. 1H HRMAS NMR was employed to investigate metabolic responses associated with the transplantation. Inflammatory markers (Interleukin-6, α2-macroglobulin) are not suitable to follow islet reactions as they are not islet specific. To study islet specific inflammatory events, immunohistochemistry was performed on sections of islet transplanted livers for thrombin (indicator of the instant blood-mediated inflammatory reaction (IBMIR)) and granulocytes and macrophages. We observed a specific correlation between IBMIR and granulocyte and macrophage infiltration after 12 h. In parallel, we identified a metabolic response associated with transplantation: after 12 h, ...
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The nature of the antibodies produced by the rabbit during the primary and secondary responses to T2 phage, proteins, and the O and H antigens of Salmonella typhosa has been determined. Immune sera have been fractionated by zone electrophoresis, sucrose density ultracentrifugation, and anion exchange chromatography. The resulting fractions have been assayed by phage neutralization or hemagglutination (antisera to proteins) or bacterial agglutination. In confirmation and extension of earlier work from this laboratory, the primary response to these antigens, with the exception of the O antigen of the Salmonella, included the early synthesis of 19S, γ-1 globulin antibody, and the later synthesis of 7S, γ-2 globulin antibody. The primary response to the O antigen consisted of the synthesis of only a macroglobulin agglutinin. The secondary response to the proteins, including the H antigen of the Salmonella, comprised the early synthesis of large amounts of the 7S γ-2 globulin antibody to the same ...
Pulmonary tuberculosis (PTB) is global disease affecting about one third of the worlds population with its attendant mortality and morbidity. Acute phase proteins have been used in monitoring the progression of infections but not in relation to PTB in this environment. The levels of total protein, albumin, α-2-macroglobulin, transferrin, and haptoglobulin were determined in 23 patients with PTB and 17- age / sex matched PTB-free controls using spectrophotometric and immunodiffusion methods respectively. The result showed that α-2-macroglobulin was significantly raised in PTB patients compared with controls ( ...
1 front of precipitation against rabbit antisera to halpha2-M. Scientific note: Serum glycoprotein of MW approx. 800,000 daltons, containing 38 histidine residues. Two protomers of MW aprox. 370,000 daltons. Each protomer is formed by two sub-units of MW 185,000 daltons, linked by disulfide bridges. N-linked complex carbohydrate side chains ...
A marked increase in serum viscosity may be responsible for many of the disease manifestations of pathologic states associated with elevated serum macroglobulin levels. For this reason, rational therapy was directed at reduction of serum viscosity and macroglobulin level.. Schwab and Fahey (New Engl. J. Med. 263: 574, 1960) have reported the beneficial effect of plasmapheresis in two patients with Waldenströms macroglobulinemia. Current evaluation of the therapeutic usefulness of plasmapheresis is based on observations in ten patients with macroglobulinemia. Many disease manifestations including bleeding diathesis, visual disturbance and retinal hemorrhages, vertigo and other neurological symptoms, and cardiac limitations were reversed ...
Prolow-density lipoprotein receptor-related protein 1 precursor (LRP)(Alpha-2-macroglobulin receptor) (A2MR) (Apolipoprotein E receptor)(APOER) (CD91 antigen) [Contains: Low-density lipoprotein receptor-related protein 1 85 kDa subunit (LRP-85); Low-density lipoproteinreceptor-related protein 1 515 kDa subunit (LRP-515); Low-densitylipoprotein receptor-related protein 1 intracellular domain (LRPICD ...
Midkine (MK or MDK) also known as neurite growth-promoting factor 2 (NEGF2) is a protein that in humans is encoded by the MDK gene. Midkine is a basic heparin-binding growth factor of low molecular weight, and forms a family with pleiotrophin (NEGF1, 46% homologous with MK). It is a nonglycosylated protein, composed of two domains held by disulfide bridges. It is a developmentally important retinoic acid-responsive gene product strongly induced during mid-gestation, hence the name midkine. Restricted mainly to certain tissues in the normal adult, it is strongly induced during oncogenesis, inflammation and tissue repair. MK is pleiotropic, capable of exerting activities such as cell proliferation, cell migration, angiogenesis and fibrinolysis. A molecular complex containing receptor-type tyrosine phosphatase zeta (PTPζ), low density lipoprotein receptor-related protein (LRP1), anaplastic leukemia kinase (ALK) and syndecans is considered to be its receptor. MK appears to enhance the angiogenic ...
LRPAP1 is involved with trafficking of certain members of the LDL receptor family including LRP1 and LRP2.The alpha-2-macroglobulin receptor complex, as purified by affinity chromatography, contains 3 polypeptides: a 515-kD heavy chain, an 85-kD light chain, and a 39-kD associated protein.The deduced amino acid sequence contains a putative signal sequence that precedes the 323-residue mature protein. The sequence showed 73% identity with a rat protein reported to be a pathogenic domain of the Heymann nephritis antigen gp330 and 77% identity to a 44-kD mouse heparin-binding protein termed HBP-44. There are also similarities between MRAP and apolipoprotein E. Studies indicated that the molecule is present on the cell surface, forming a complex with the heavy and light chains of the alpha-2-macroglobulin receptor.
TY - JOUR. T1 - Expression of α2-macroglobulin by the interaction between hepatocytes and endothelial cells in coculture. AU - Talamini, Mark A.. AU - McCluskey, Michael P.. AU - Buchman, Timothy G.. AU - De Maio, Antonio. PY - 1998/7. Y1 - 1998/7. N2 - The interaction between distinct cell types within the liver seems to be important in regulating hepatic function. However, these interactions have not been well characterized because of difficulty in reproducing the hepatic environment in an ex vivo model. In the present study a coculture system of hepatocytes and endothelial cells was established to investigate the communication between parenchymal and nonparenchymal cells. Freshly isolated rat hepatocytes were placed onto a monolayer of primary aortic rat endothelial cells. Analysis of the proteins secreted into the extracellular medium after pulse labeling with radioactive amino acids revealed the presence of a 180,000-apparent molecular weight glycoprotein, BBB-180, which was not detected ...
α2-Macroglobulin (α2M) is an extracellular chaperone that inhibits amorphous and fibrillar protein aggregation. The reaction of α2M with proteases results in an activated conformation, where the proteases become covalently-linked within the interior of a cage-like structure formed by α2M. This study investigates, the effect of activation on the ability of α2M to inhibit amyloid formation by Aβ1-42 and I59T human lysozyme and shows that protease-activated α2M can act via two distinct mechanisms: (i) by trapping proteases that remain able to degrade polypeptide chains and (ii) by a chaperone action that prevents misfolded clients from continuing along the amyloid forming pathway.
Note: Optimal annealing temperatures are often higher than the Tm of the primers (approximately +5 to +10°C) and have to be determined empirically. For both primers, the Tm should be similar.. Bases that do not hybridize to the template may be added at the 5´ end of a primer (e.g., for introducing restriction sites into the amplification product). Primer concentrations between 0.1 and 0.6 µM are generally optimal. Higher primer concentrations may promote mispriming and accumulation of nonspecific product. Lower primer concentrations may be exhausted before the reaction is completed, resulting in lower yields of desired product.. Note: For some systems, a higher primer concentration (up to 1 µM) may improve results. When testing new primers, always include a positive control reaction with a template that has been tested for function in PCR. This control shows whether the primers are working. The Human Genomic DNA from Roche is a good control template for evaluation of human primer ...
May regulate the interaction between the 3M complex and the histone deacetylases HDAC4 and HDAC5, PubMed:25752541. May also regulate LRP2/megalin, By similarity. {ECO:0000250,UniProtKB:A2ARV4, ECO:0000269,PubMed:25752541 ...
Amazone will be at its usual spot on Stand 4, just beside the Sprays & Sprayers ring at Cereals.. Showing for the first time in the UK will be the Cayron 200VS - 100 auto reset plough with pressure-adjustable hydraulic stone release for maximum protection. There are also two new body designs for heavy land, the W35 helical body and the S35 scrolled body. Cenius 03 series mounted mulch cultivators in 3.0m, 3.5m and 4.0m are now fitted with the new C-Mix stone safety protection system giving depth guidance as well as protection against stone damage.. Cirrus 4003-2C ISOBUS cultivator drill brings the grain and fertiliser option now down to a narrower working width making it ideal for the spring cereal grower. The segmented distribution head means half-side shut-off from the tractor seat or via GPS SectionControl.. ...
Item #: SCP-001. Object class: Euclid. Special containment procedures: SCP-001 is to be guarded by highly-trained personnel from the ████ ████████ Special Forces division, the ████ ████, and members of █████████ ████. Electric fences of height 9 m are to be maintained around the areas spatial divides. Intruders are to be shot on sight, as the contents of the facility built on SCP-001 are highly sensitive. Level O5 personnel are barred from entry due to their sensitive position. Various standard security features are omitted for reasons of brevity, and can be found in Document 19-0001. ANY SATELLITE FOOTAGE CONTAINING PICTURES OF SCP-001 IS TO BE EXCISED IMMEDIATELY BY EMBEDDED AGENT █████████ ████████████ [OP-0041] AND DESTROYED.. Description: SCP-001 consists of a square area of approximately 24 km. This land, and all matter on it, shift geographical location at approximately two-week ...
The H11G1M and H11G2M are photodarlington-type optically coupled optocouplers. These devices have a gallium arsenide infrared emitting diode coupled with a si
Part 1 of this three-part series provides an in-depth look at the history component. By Raemarie Jimenez, CPC, CPC-I, CANPC, CRHC Although evaluation and
Complete information for A2ML1 gene (Protein Coding), Alpha-2-Macroglobulin Like 1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
TY - JOUR. T1 - Serum alpha-2-macroglobulin, antitrypsin and antichymotrypsin activities in patients receiving treatment with cyclosporine. AU - Roche, Maya. AU - Kusumanjali, G.. AU - Chinnapu Reddy, G.. AU - Kanagasabhapathy, A. S.. AU - Rao, Pragna. PY - 2006. Y1 - 2006. N2 - Cyclosporine has been reported to function as an inhibitor of the chymotrypsin like activity of proteasome. We hypothesized that the administration of an exogenous proteinase inhibitor may affect the activities of the naturally occurring serum anti proteinases. The aim of this study was to observe the pattern of alteration of serum alpha 2 macroglobulin (AMG), alpha 1- antitrypsin (AT) and alpha 1-antichymotrypsin (ACT) activities in renal transplant patients receiving the immunosuppressive drug, cyclosporine. Patients (97) who had received a single renal allograft were inducted into the study. Subjects were on a twice-daily dosage of cyclosporine capsules. Trough (Co) and two-hour post dose (C 2) cyclosporine levels ...
The low-density lipoprotein (LDL) receptor plays a central role in mammalian cholesterol metabolism, clearing lipoproteins which bear apolipoproteins E and B-100 from plasma. Mutations in this molecule are associated with familial hypercholesterolemia, a condition which leads to an elevated plasma cholesterol concentration and accelerated atherosclerosis. The N-terminal segment of the LDL receptor contains a heptad of cysteine-rich repeats that bind the lipoproteins. Similar repeats are present in related receptors, including the very low-density lipoprotein receptor and the LDL receptor-related protein/alpha 2-macroglobulin receptor, and in proteins which are functionally unrelated, such as the C9 component of complement. The first repeat of the human LDL receptor has been expressed in Escherichia coli as a glutathione S-transferase fusion protein, and the cleaved and purified receptor module has been shown to fold to a single, fully oxidized form that is recognized by the monoclonal antibody ...
BACKGROUND: Blood coagulation occurs by a cascade of zymogen activation resulting from minor proteolysis. The final stage of coagulation involves thrombin generation and limited proteolysis of fibrinogen to give spontaneously polymerizing fibrin. The resulting fibrin network is covalently crosslinked by factor XIIIa to yield a stable blood clot. Fibrinogen is a 340 kDa glycoprotein composed of six polypeptide chains, (alphabetagamma)2, held together by 29 disulfide bonds. The globular C terminus of the gamma chain contains a fibrin-polymerization surface, the principal factor XIIIa crosslinking site, the platelet receptor recognition site, and a calcium-binding site. Structural information on this domain should thus prove helpful in understanding clot formation. RESULTS: The X-ray crystallographic structure of the 30 kDa globular C terminus of the gamma chain of human fibrinogen has been determined in one crystal form using multiple isomorphous replacement methods. The refined coordinates were ...
Nykjaer A, Petersen CM, Moller B, Jensen PH, Moestrup SK, Holtet TL, Etzerodt M, Thogersen HC, Munch M, Andreasen PA, et al.: Purified alpha 2-macroglobulin receptor/LDL receptor-related protein binds urokinase.plasminogen activator inhibitor type-1 complex. Evidence that the alpha 2-macroglobulin receptor mediates cellular degradation of urokinase receptor-bound complexes. J Biol Chem. 1992 Jul 25;267(21):14543-6. [PubMed:1378833 ...
[106 Pages Report] Check for Discount on Global Plasma Protease C1-inhibitor Treatment Sales Market Report 2017 report by QYResearch Group. In this report, the global Plasma Protease C1-inhibitor Treatment...
Torturing turpentine, I thought that would be a fabulous name for a novel, likely autobiographical. I spend a great deal of time torturing turpentine which I must admit is a far better choice than actually drinking it. Dont drink the blue kool-aid, people. Its a term that comes from endless political debates, especially on the…
Scanning electron microscopy of tests of Triticites ventricosus and Schwagerina sp. shows that the microgranular wall was secreted rather than agglutinated. Grains range in size from 0.5 to 6.0 μm, but grains larger than 4 μm are rare. Evidence that grains were secreted includes tight packing, uniform size and shape, and apparently homogeneous composition. Furthermore, scanning electron microscopy confirms the perforate nature of the antetheca and the nature of the keriothecal wall. Alveoli taper toward the tectum and pass through the tectum as tiny pores, enhancing communication between chambers and with the external environment.. The traditional model for the addition of chambers is rejected. Three ways in which new chambers may have been added to the test that are consistent with observations made here: 1) The antetheca may have thickened differentially, creating a substrate to which the keriotheca of the new chamber was attached; 2) Part of the tectum, septum, and keriotheca of the ...
In progress notes after the jump. .... Cha cha -- all to the same song -- and freestyle night. As Tom and Samantha talk, Jason and Cristian urge the crowd to greater frenzy. Drawing for order. Cristian first, Jason second, Kristi third. Practice videos, including group rehearsal where they can see each others routines. Cristian and Cheryl. Very disco. Dancing on the Ceiling. Good steps and Cheryl is working it. But Cristian still favors the arm. Enter Jason and Edyta. Slinky. A little more formal than Cristian and Cheryl, more guarded in the movements -- until Jason leapfrogs Edyta. Still not as good as C/C. And now Kristi and Mark. Theres no contest. K&M are killing. Group reforms, ending with Jason and Cristian lifting Kristi over their heads, which is where she belongs. Len praises everyone, but doesnt feel that Latin is Jasons thing -- and Kristi is the complete package. Bruno says they all did well; praises Cristians improvement…
Novel compounds of formula (I), in which R is R1, R1 O or R1 COO, where R1 is C1-12 alkyl, n and m are 0 or 1 provided that (n+m) is 0 or 1, each of a, b, c and d are independently 0 or 1, provided (a+b+c+d) is not zero, but excluding the case where a and b are 0 and m is 0 and one of c or d is 0 are described, being useful constituents of nematic liquid crystal materials.
General Mills also this week signed the CEO Water Mandate, a public-private initiative launched in 2007 by UN Secretary-General Ban Ki-moon to encourage companies to develop, implement and disclose water sustainability policies and practices.. Two thirds of the worlds largest companies - including Merck, Unilever and H&M - are reporting exposure to water risks, some of which have potential to limit growth, according to CDPs latest annual global water report published earlier this month.. ...
Just a quick update.. I got an email from my surrogacy coordinator on Monday... E&M are having their physician review my medical records! :) Im hoping thats good news in regards to moving forward.. :) *Fingers Crossed* Until the next update....
As the recent spate of deal-making in pharma continues, Merck & Co said it is shelling out $100 million for access to Modernas messenger RNA Therapeutics platform to develop new antiviral vaccines and passive immunity therapies. - News - PharmaTimes
Billing E&M level with mod 25 and procedure 97597; no wound closure, just a dressing application. Medicare is paying the E&M but not processin
The alpha 2-macroglobulin (alpha 2M) receptor/low-density lipoprotein receptor-related protein (LRP) is important for the clearance of proteases, protease-inhibitor complexes, and various ligands associated with lipid metabolism. While the regulation of receptor function is poorly understood, the addition of high concentrations of the 39-kD receptor-associated protein (RAP) to cells inhibits the binding and/or uptake of many of these ligands. Previously, we (Kounnas, M.Z., R.E. Morris, M.R. Thompson, D.J. FitzGerald, D.K. Strickland, and C.B. Saelinger. 1992. J. Biol. Chem. 267:12420-12423) [corrected] showed that Pseudomonas exotoxin (PE) could bind immobilized LRP. Also, the addition of RAP blocked toxin-mediated cell killing. These findings suggested that PE might use LRP to gain entry into toxin-sensitive cells. Here we report on a strategy to select PE-resistant lines of Chinese hamster ovary cells that express altered amounts of LRP. An important part of this strategy is to screen ...
3.3.co;2-i. PMID 11425005. Aldahmesh MA, Khan AO, Alkuraya H, Adly N, Anazi S, Al-Saleh AA, Mohamed JY, Hijazi H, Prabakaran S, Tacke M, Al-Khrashi A, Hashem M, Reinheckel T, Assiri A, Alkuraya FS. "Mutations in LRPAP1 Are Associated with Severe Myopia in Humans". Am J Hum Genet. 93: 313-20. doi:10.1016/j.ajhg.2013.06.002. PMC 3738831 . PMID 23830514. Aldahmesh MA, Khan AO, Alkuraya H, Adly N, Anazi S, Al-Saleh AA, Mohamed JY, Hijazi H, Prabakaran S, Tacke M, Al-Khrashi A, Hashem M, Reinheckel T, Assiri A, Alkuraya FS (2013). "Mutations in LRPAP1 are associated with severe myopia in humans". American Journal of Human Genetics. 93 (2): 313-20. doi:10.1016/j.ajhg.2013.06.002. PMC 3738831 . PMID 23830514. Willnow TE, Armstrong SA, Hammer RE, Herz J (1995). "Functional expression of low density lipoprotein receptor-related protein is controlled by receptor-associated protein in vivo". Proceedings of the National Academy of Sciences of the United States of America. 92 (10): 4537-41. ...
Myasthenia gravis (MG) and the Lambert-Eaton myasthenic syndrome (LEMS) are the prototypical autoimmune channelopathies of the peripheral nervous system. The predominant neuromuscular junction antigenic targets are either postsynaptic (MG; nicotinic acetylcholine receptor (AChR)) or presynaptic (LEMS; voltage-gated calcium channels (VGCCs)). Over the last 35 years the development of active immunization, passive transfer models, and use of transgenic animals has greatly enhanced our understanding of the biology of these disorders. The search for other antibodies in previously seronegative MG cases has led to the recognition of antibodies against clustered AChRs, muscle-specific kinase (MuSK) and low density lipoprotein receptor-related protein 4 (Lrp4). The pathogenic mechanisms that underlie these newer serological subclasses of MG are not yet fully understood, but are likely to be different from AChR antibody-mediated disease. While many still would consider MG an antibody-mediated disorder, it is
A macroglobulin is used to improve the signal-to-background ratio in an affinity binding assay employing a proteinase (or precursor) as a label. The macroglobulin entraps unbound labeled reagent, thereby reducing its signal generating activity, relative to that of the affinity complex.
Opportunities exist for up to two postdoctoral scholars, available August 1, 2017, to investigate innate immunity in insects, its role in development, and in the transmission of infectious diseases. You will join an interdisciplinary group using biochemistry, biophysics, structural biology, and cell-based assays in collaboration with experts at the organismal level. Your objective will be to elucidate novel molecular mechanisms of immunity, and new connections between invertebrate and human immune responses, that can be leveraged to predict and disrupt disease transmission. For more information, visit http://baxterlab.yale.edu.. Applicants should have a Ph.D. in chemistry, biophysics, biochemistry, molecular biology, or related discipline, record of peer-reviewed publication, and familiarity with standard laboratory techniques such as PCR/molecular biology, protein expression and purification, and analysis of proteins and nucleic acids by spectroscopy, electrophoresis, etc. The following skills ...
The nonsense-mediated mRNA decay (NMD) pathway is a cellular quality control and post-transcriptional gene regulatory mechanism and is essential for viability in most multicellular organisms. A complex of proteins has been identified to be required for NMD function to occur, however there is an incomplete understanding of the individual contributions of each of these factors to the NMD process. Central to the NMD process are three proteins, Upf1 (SMG-2), Upf2 (SMG-3), and Upf3 (SMG-4), which are found in all eukaryotes, with Upf1 and Upf2 being absolutely required for NMD in all organisms in which their functions have been examined. The other known NMD factors, Smg1, Smg5, Smg6, and Smg7 are more variable in their presence in different orders of organisms and are thought to have a more regulatory role. Here we present the first genetic analysis of the NMD factor Smg5 in Drosophila. Surprisingly, we find that unlike the other analyzed Smg genes in this organism, Smg5 is essential for NMD ...
Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription (By similarity).
The disinfectant cleaners from 3M are concentrated products to be diluted and dispensed with the 3M Twistn Fill dilution system. These products does not need to be rinsed and has a pleasant fragrance.
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Publishers Clifford and Stuart Richner announced July 10 that Richner Communications has agreed to acquire L&M Publications, a Long Island weekly newspaper group that includes the Merrick Life, Bellmore Life, Freeport-Baldwin Leader and the Wantagh-Seaford Citizen. Both Richner Communications and L&M are privately held companies, and terms of the sale were not announced. The sale is expected to close this quarter.
There are no specific protocols for Recombinant Human alpha 1 Antitrypsin protein (ab79181). Please download our general protocols booklet
TY - JOUR. T1 - FZD7 has a critical role in cell proliferation in triple negative breast cancer. AU - Yang, L.. AU - Wu, X.. AU - Wang, Y.. AU - Zhang, K.. AU - Wu, J.. AU - Yuan, Y. C.. AU - Deng, X.. AU - Chen, L.. AU - Kim, C. C.H.. AU - Lau, S.. AU - Somlo, G.. AU - Yen, Y.. PY - 2011/10/27. Y1 - 2011/10/27. N2 - Breast cancer is genetically and clinically heterogeneous. Triple negative breast cancer (TNBC) is a subtype of breast cancer that is usually associated with poor outcome and lack of benefit from targeted therapy. We used microarray analysis to perform a pathway analysis of TNBC compared with non-triple negative breast cancer (non-TNBC). Overexpression of several Wnt pathway genes, such as frizzled homolog 7 (FZD7), low density lipoprotein receptor-related protein 6 and transcription factor 7 (TCF7) was observed in TNBC, and we directed our focus to the Wnt pathway receptor, FZD7. To validate the function of FZD7, FZD7shRNA was used to knock down FZD7 expression. Notably, reduced ...
3 Zhang C, An J ܣ , Haile WB, Echeverry R, Strickland DK, Yepes M. Zhang C, An J, Haile WB, Echeverry R, Strickland DK, Yepes M. Zhang C, An J, Haile WB, Echeverry R, Strickland DK, Yepes M. Microglial low-density lipoprotein receptor-related protein 1 mediates the effect of tissue-type plasminogen activator on matrix metalloproteinase-9 activity in the ischemic brain. J Cereb Blood Flow Metab. 2009 Dec;29(12):1946-54. Ӱ??????5.741 ͬ ?? ???? pubmed ...
Internalization of the urokinase-type plasminogen activator (uPA) requires two receptors, the uPA receptor (uPAR) and the low density lipoprotein receptor-related protein (LRP)/alpha2-macroglobulin (alpha2M) receptor. Here, we address whether protein kinases are involved in the internalization of uPA by human melanoma cells. Initially, we found that the internalization of uPA was significantly inhibited by the serine/threonine protein kinase inhibitors staurosporine, K-252a and H-89, but not by the tyrosine kinase inhibitors, genistein and lavendustin A. Internalization of uPA was also inhibited by a pseudosubstrate peptide for cAMP-dependent protein kinase (PKA), but not by a pseudosubstrate peptide for protein kinase C. We confirmed a requirement for PKA-activity and implicated a specific isoform by using an antisense oligonucleotide against the regulatory subunit RI alpha of PKA which suppresses PKA-I activity. Exposure of cells to this oligonucleotide led to a specific, dose-dependent ...

Ligand binding, conformational change and plasma elimination of human, mouse and rat α-macroglobulin proteinase inhibitors |...Ligand binding, conformational change and plasma elimination of human, mouse and rat α-macroglobulin proteinase inhibitors |...

Rat alpha 1-macroglobulin (alpha 1M), rat alpha 2-macroglobulin (alpha 2M) migrated as single bands on non-denaturing gels when ... rat alpha 1M, rat alpha 2M and mouse alpha 2M, respectively. Only rat alpha 2M, the acute-phase alpha-macroglobulin studied, ... The other alpha-macroglobulin-methylamine complexes cleared faster than the inhibitors that had not reacted. Rat alpha 2M and ... A single saturable pathway rapidly cleared complexes of trypsin and the alpha-macroglobulins of mouse, rat and human from the ...
more infohttp://www.biochemj.org/content/209/1/99

TNO Repository search for: subject:alpha-MacroglobulinsTNO Repository search for: subject:'alpha-Macroglobulins'

alpha 2 macroglobulin · biological marker · gelatinase A · gelatinase B · hydroxyproline · interstitial collagenase · matrix ... Biomedical Research · alpha 2 macroglobulin · interstitial collagenase · matrix metalloproteinase · prointerstitial collagenase ... Chemicals / CAS: alpha 2 macroglobulin, 95568-41-5; gelatinase A, 146480-35-5; gelatinase B, 146480-36-6; hydroxyproline, 51-35 ... A2-antiplasmin · Activity assay · Automated · Plasmin inhibitor · Adult · alpha-Macroglobulins · Antifibrinolytic Agents · ...
more infohttps://repository.tudelft.nl/search/tno/?q=subject%3A%22alpha-Macroglobulins%22

Alpha-2-MacroglobulinAlpha-2-Macroglobulin

... ,ARUP Laboratories is a national reference laboratory and a worldwide leader in innovative laboratory ...
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Upregulation of Alpha-2-Macroglobulin in Replicative Senescence | SpringerLinkUpregulation of Alpha-2-Macroglobulin in Replicative Senescence | SpringerLink

Ma H, Li RZ, Zhang ZY, Tong TJ (2004) mRNA level of alpha-2-macroglobulin as an aging biomarker of human fibroblasts in culture ... Alpha-2-macroglobulin (α2Μ) Electrophoretic mobility shift assays (EMSA) Functional promoter region determination α2M ... Ma L.W., Li G.D., Tong T.J. (2014) Upregulation of Alpha-2-Macroglobulin in Replicative Senescence. In: Hayat M. (eds) Tumor ... Lin VK, Wang SY, Boetticher NC, Vazquez DV, Saboorian H, McConnell JD, Roehrborn CG (2005) Alpha(2) macroglobulin, a PSA ...
more infohttps://link.springer.com/chapter/10.1007/978-94-007-7726-2_8

alpha-Macroglobulins: detection and characterization - Research - Aarhus Universityalpha-Macroglobulins: detection and characterization - Research - Aarhus University

alpha-Macroglobulins: detection and characterization. Research output: Contribution to journal/Conference contribution in ...
more infohttps://pure.au.dk/portal/en/publications/alphamacroglobulins-detection-and-characterization

Alpha-2-Macroglobulin - WikipediaAlpha-2-Macroglobulin - Wikipedia

The loss of alpha-2-macroglobulin into urine is prevented by its large size. The net result is that alpha-2-macroglobulin ... Overall, alpha-2-Macroglobulin is the largest major nonimmunoglobulin protein in human plasma. The alpha-macroglobulin (aM) ... 10-15% of copper in human plasma is chelated by alpha-2-macroglobulin. alpha-2-Macroglobulin levels are increased when the ... of alpha-2-macroglobulin leads to increased risk of Alzheimers disease. alpha-2-Macroglobulin binds to and removes the active ...
more infohttps://en.wikipedia.org/wiki/Alpha-2-Macroglobulin

Natural Human alpha 2 Macroglobulin protein (ab91104)Natural Human alpha 2 Macroglobulin protein (ab91104)

Buy our Natural Human alpha 2 Macroglobulin protein. Ab91104 is a full length protein produced in Nativesyntheticaly and has ... Lane 4. 5 µg alpha 2 Macroglobulin (reduced/ heated) Lane 5. 10 µg alpha 2 Macroglobulin (reduced/ heated) Lane 6. 20 µg alpha ... 5 µg alpha 2 Macroglobulin (non-reduced/no heat) Lane 2. 10 µg alpha 2 Macroglobulin (non-reduced/no heat) Lane 3. 20 µg alpha ... Natural Human alpha 2 Macroglobulin protein. See all alpha 2 Macroglobulin proteins and peptides. ...
more infohttp://www.abcam.com/natural-human-alpha-2-macroglobulin-protein-ab91104.html

alpha 2-Macroglobulin Lysates: Novus Biologicalsalpha 2-Macroglobulin Lysates: Novus Biologicals

Browse our alpha 2-Macroglobulin Lysate catalog backed by our Guarantee+. ... alpha 2-Macroglobulin lysate, A2M lysate, ALPL lysate, alpha-2-M lysate, alpha-2-macroglobulin lysate, C3 and PZP-like alpha-2- ... alpha 2-Macroglobulin Lysates. We offer alpha 2-Macroglobulin Lysates for use in common research applications: Western Blot. ... Our alpha 2-Macroglobulin Lysates can be used in a variety of model species: Human. Use the list below to choose the alpha 2- ...
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alpha 2-Macroglobulin Proteins: Novus Biologicalsalpha 2-Macroglobulin Proteins: Novus Biologicals

Browse our alpha 2-Macroglobulin Protein catalog backed by our Guarantee+. ... alpha 2-Macroglobulin Proteins. We offer alpha 2-Macroglobulin Peptides and alpha 2-Macroglobulin Proteins for use in common ... alpha 2-Macroglobulin protein, A2M protein, ALPL protein, alpha-2-M protein, alpha-2-macroglobulin protein, C3 and PZP-like ... Our alpha 2-Macroglobulin Peptides and alpha 2-Macroglobulin Proteins can be used in a variety of model species: Human. Use the ...
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Alpha-2-macroglobulin, thiol-ester bond-forming (IPR019565) | InterPro | EMBL-EBIAlpha-2-macroglobulin, thiol-ester bond-forming (IPR019565) | InterPro | EMBL-EBI

A conserved region in alpha-macroglobulins participates in binding to the mammalian alpha-macroglobulin receptor.. Biochemistry ... Proteinase binding and inhibition by the monomeric alpha-macroglobulin rat alpha 1-inhibitor-3.. J. Biol. Chem. 264 11428-35 ... The alpha-macroglobulin (aM) family of proteins includes protease inhibitors [PMID: 2473064], typified by the human tetrameric ... Alpha-macroglobulins: structure, shape, and mechanism of proteinase complex formation.. J. Biol. Chem. 264 11539-42 1989 ...
more infohttp://www.ebi.ac.uk/interpro/entry/IPR019565

Anti-alpha 2 Macroglobulin antibody (HRP) (ab7339) ProtocolsAnti-alpha 2 Macroglobulin antibody (HRP) (ab7339) Protocols

Abcam provides specific protocols for Anti-alpha 2 Macroglobulin antibody (HRP) (ab7339) : Immunohistochemistry protocols, ...
more infohttp://www.abcam.com/alpha-2-macroglobulin-antibody-hrp-ab7339-protocols.html

anti-alpha 2 Macroglobulin antibody [N2N3]  | GeneTexanti-alpha 2 Macroglobulin antibody [N2N3] | GeneTex

... alpha-2-macroglobulin) for WB. Anti-alpha 2 Macroglobulin pAb (GTX112856) is tested in Human samples. 100% Ab-Assurance. ... alpha-2-macroglobulin antibody, alpha-2-M antibody, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 antibody ... alpha-2-macroglobulin. Background. Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many ... Storage Conditions: alpha 2 Macroglobulin antibody [N2N3]. Storage Buffer. 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal ...
more infohttp://www.genetex.com/alpha-2-Macroglobulin-antibody-N2N3-GTX112856.html

Alpha 2-macroglobulin | definition of Alpha 2-macroglobulin by Medical dictionaryAlpha 2-macroglobulin | definition of Alpha 2-macroglobulin by Medical dictionary

What is Alpha 2-macroglobulin? Meaning of Alpha 2-macroglobulin medical term. What does Alpha 2-macroglobulin mean? ... Looking for online definition of Alpha 2-macroglobulin in the Medical Dictionary? Alpha 2-macroglobulin explanation free. ... alpha-2 macroglobulin. (redirected from Alpha 2-macroglobulin). Also found in: Wikipedia. alpha-2 macroglobulin. Plasma ... Structure of the human alpha 2-macroglobulin gene and its promoter.. Alpha 2 macroglobulin activity in rats infected with ...
more infohttps://medical-dictionary.thefreedictionary.com/Alpha+2-macroglobulin

Alpha-2 Macroglobulin (A2M), anti-Human Po | LeeBio.comAlpha-2 Macroglobulin (A2M), anti-Human Po | LeeBio.com

Buy goat anti-human Alpha 2-Macroglobulin polyclonal antibody for research, development and IVD manufacturing uses - Inquire ... Polyclonal anti-human alpha 2 macroglobulin (a2M) procedure goats were immunized with highly purified human alpha 2- ... macroglobulin isolated from human serum. The resulting anti-human alpha-2 Macroglobulin (a2M) was solid phase absorbed then ... This anti-human alpha 2-macroglobulin (A2M) is suitable for depletion, ELISA and Blotting applications. ...
more infohttps://www.leebio.com/product/1566/alpha-2-macroglobulin-a2m-goat-anti-human-polyclonal-antibody-affinity-purified-g2m-80a

BKCa channel regulates calcium oscillations induced by alpha-2-macroglobulin in human myometrial smooth muscle cells | PNASBKCa channel regulates calcium oscillations induced by alpha-2-macroglobulin in human myometrial smooth muscle cells | PNAS

Characterization of alpha 2-macroglobulin binding to human trabecular meshwork cells: Presence of the alpha 2-macroglobulin ... 1992) Alpha 2-macroglobulin, a multifunctional binding protein with targeting characteristics. FASEB J 6(15):3345-3353. ... 2006) Lack of site-specific production of decidual alpha-2 macroglobulin in human pregnancy. J Soc Gynecol Investig 13(7):491- ... 1994) Expression of the alpha 2-macroglobulin-encoding gene in rat brain and cultured astrocytes. Gene 141(2):155-162. ...
more infohttp://www.pnas.org/content/113/16/E2335

Alpha-2-macroglobulin Antibody 13545-1-AP  | ProteintechAlpha-2-macroglobulin Antibody 13545-1-AP | Proteintech

Alpha-2-macroglobulin Antibody 13545-1-AP has been identified with IF, IHC, IP, WB, ELISA. 13545-1-AP detected 185 kDa band in ... alpha-2-macroglobulin, also known as α2-macroglobulin (α2M or A2M), is a protein abundant in the plasma of vertebrates and ... A2M, Alpha 2 M, alpha 2 macroglobulin, CPAMD5, FWP007, S863 7, α-2M ... Alpha-2-macroglobulin Antibody 0 Publications. Rabbit Polyclonal, Catalog number: 13545-1-AP ...
more infohttps://www.ptglab.com/Products/A2M-Antibody-13545-1-AP.htm

Complement C5 (C3 And PZP Like Alpha 2 Macroglobulin Domain Containing Protein 4 or C5) - Pipeline Review, H2 2017Complement C5 (C3 And PZP Like Alpha 2 Macroglobulin Domain Containing Protein 4 or C5) - Pipeline Review, H2 2017

... Download the ... 12, 2017 /PRNewswire/ -- Complement C5 (C3 And PZP Like Alpha 2 Macroglobulin Domain Containing Protein 4 or C5) - Pipeline ... Complement C5 (C3 And PZP Like Alpha 2 Macroglobulin Domain Containing Protein 4 or C5) - Pipeline Review, H2 2017. Friday, ... It also reviews key players involved in Complement C5 (C3 And PZP Like Alpha 2 Macroglobulin Domain Containing Protein 4 or C5 ...
more infohttp://www.medindia.net/health-press-release/Complement-C5-C3-And-PZP-Like-Alpha-2-Macroglobulin-Domain-Containing-Protein-4-or-C5-Pipeline-Review-H2-2017--344249-1.htm

RCSB PDB - 4ZIU: Crystal structure of native alpha-2-macroglobulin from Escherichia coli spanning the residues from domain MG7...RCSB PDB - 4ZIU: Crystal structure of native alpha-2-macroglobulin from Escherichia coli spanning the residues from domain MG7...

Crystal structure of native alpha-2-macroglobulin from Escherichia coli spanning the residues from domain MG7 to the C-terminus ... Crystal structure of native alpha-2-macroglobulin from Escherichia coli spanning the residues from domain MG7 to the C-terminus ... Structural and functional insights into Escherichia coli alpha 2-macroglobulin endopeptidase snap-trap inhibition.. Garcia- ... Gram-negative bacteria from the human gut microbiome encode a relative of the human endopeptidase inhibitor, α2-macroglobulin ( ...
more infohttps://www.rcsb.org/structure/4ZIU

Effect of alpha-2-macroglobulin on cytokine-mediated human C-reactive protein production | SpringerLinkEffect of alpha-2-macroglobulin on cytokine-mediated human C-reactive protein production | SpringerLink

Alpha-2-macroglobulin (α2-M), a serum protease inhibitor that also binds cytokines, neutralized the inhibitory effect exerted ... Effect of alpha-2-macroglobulin on cytokine-mediated human C-reactive protein production. *Andrew W. Taylor1. & ... Alpha-2-macroglobulin (α2-M), a serum protease inhibitor that also binds cytokines, neutralized the inhibitory effect exerted ... Taylor, A.W., Mortensen, R.F. Effect of alpha-2-macroglobulin on cytokine-mediated human C-reactive protein production. ...
more infohttps://rd.springer.com/article/10.1007%2FBF00917910

Prostate-specific antigen forms complexes with human alpha 2-macroglobulin and binds to the alpha 2-macroglobulin receptor/LDL...Prostate-specific antigen forms complexes with human alpha 2-macroglobulin and binds to the alpha 2-macroglobulin receptor/LDL...

To investigate the binding of the prostate-specific antigen (PSA) to human alpha 2-macroglobulin (alpha 2-M) and to alpha 1- ... Prostate-specific antigen forms complexes with human alpha 2-macroglobulin and binds to the alpha 2-macroglobulin receptor/LDL ... Complexes of PSA-alpha 2-M and PSA-ACT were found to bind to the alpha 2-macroglobulin receptor/LDL receptor-related protein ( ... Kinetic analysis revealed faster binding of PSA to alpha 2-M than to ACT. The PSA bound to alpha 2-M is caged by the inhibitor ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/9400498?dopt=Abstract

A Role for Alpha-2-Macroglobulin in the Cancer Immunity of Naked Mole-Rats - Fight Aging!A Role for Alpha-2-Macroglobulin in the Cancer Immunity of Naked Mole-Rats - Fight Aging!

A Role for Alpha-2-Macroglobulin in the Cancer Immunity of Naked Mole-Rats. Permalink No Comments Yet Add a Comment Posted by ... Here researchers outline a role for alpha-2-macroglobulin (A2M) in cancer suppression; it appears to inhibit tumor growth in ...
more infohttps://www.fightaging.org/archives/2018/01/a-role-for-alpha-2-macroglobulin-in-the-cancer-immunity-of-naked-mole-rats/

A Role for Alpha-2-Macroglobulin in the Cancer Immunity of Naked Mole-Rats - Fight Aging!A Role for Alpha-2-Macroglobulin in the Cancer Immunity of Naked Mole-Rats - Fight Aging!

A Role for Alpha-2-Macroglobulin in the Cancer Immunity of Naked Mole-Rats. Permalink No Comments Yet Add a Comment Posted by ... Here researchers outline a role for alpha-2-macroglobulin (A2M) in cancer suppression; it appears to inhibit tumor growth in ...
more infohttps://www.fightaging.org/archives/2018/01/a-role-for-alpha-2-macroglobulin-in-the-cancer-immunity-of-naked-mole-rats

Rat Alpha-2 Macroglobulin (A2M) - Innovative ResearchRat Alpha-2 Macroglobulin (A2M) - Innovative Research

Alpha-2 macroglobulin (A2M) is a large plasma protein found in the blood. A2M is produced by the liver, and is a major ... component of the alpha-2 band in protein elec ... Rat Alpha-2 Macroglobulin, Alpha-2 macroglobulin (A2M) is a ... studies showed that the comparison of the amino acid compositions of Alpha-2 macroglobulin from Rat and Alpha-2 macroglobulin ... It was reported that the conformation of rat acute-phase Alpha-2 macroglobulin , both at the molecular and secondary structural ...
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Analysis of Alpha-2 Macroglobulin from the Long-Lived and Cancer-Resistant Naked Mole-Rat and Human Plasma.  - PubMed - NCBIAnalysis of Alpha-2 Macroglobulin from the Long-Lived and Cancer-Resistant Naked Mole-Rat and Human Plasma. - PubMed - NCBI

Analysis of Alpha-2 Macroglobulin from the Long-Lived and Cancer-Resistant Naked Mole-Rat and Human Plasma.. Thieme R1, Kurz S1 ... Analysis of Alpha-2 Macroglobulin from the Long-Lived and Cancer-Resistant Naked Mole-Rat and Human Plasma ... Analysis of Alpha-2 Macroglobulin from the Long-Lived and Cancer-Resistant Naked Mole-Rat and Human Plasma ... Analysis of Alpha-2 Macroglobulin from the Long-Lived and Cancer-Resistant Naked Mole-Rat and Human Plasma ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/26103567?dopt=Abstract

Inhibition of Entamoeba Histolytica Cytotoxin by Alpha 1 Antiprotease and Alpha 2 Macroglobulin* | The American Journal of...Inhibition of Entamoeba Histolytica Cytotoxin by Alpha 1 Antiprotease and Alpha 2 Macroglobulin* | The American Journal of...

Alpha-2 macroglobulin was purified and alpha-1 antiprotease was partially purified from human sera by other methods and shown ... A high molecular weight inhibitory fraction was identified as predominantly alpha-2 macroglobulin and a low molecular weight ... Preparative isoelectric focusing of human serum isolated inhibitory fractions containing these same alpha globulins. ... inhibitory fraction was identified as predominantly alpha-1 antiprotease. ...
more infohttp://www.ajtmh.org/content/journals/10.4269/ajtmh.1981.30.575
  • Binding of PSA to alpha 2-M is initiated by the cleavage of the peptide bond between amino acids Tyr 686 and Glu 687 of the bait region indicating a chymotrypsin-like activity of the PSA. (nih.gov)
  • 4ZIU: Crystal structure of native alpha-2-macroglobulin from Escherichia coli spanning the residues from domain MG7 to the C-terminus. (rcsb.org)
  • Since it is known that the NMR expresses higher liver mRNA-levels of alpha 2-macroglobulin than mice, nothing is known about its structure, functionality or expression level in the NMR compared to the human A2M. (nih.gov)
  • The cleavage site of PSA in alpha 2-M was analyzed by SDS-PAGE and microsequencing. (nih.gov)
  • Each alpha 2-Macroglobulin Lysate is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. (novusbio.com)