Serum globulins with high molecular weight. (Dorland, 28th ed)
All blood proteins except albumin ( = SERUM ALBUMIN, which is not a globulin) and FIBRINOGEN (which is not in the serum). The serum globulins are subdivided into ALPHA-GLOBULINS; BETA-GLOBULINS; and GAMMA-GLOBULINS on the basis of their electrophoretic mobilities. (From Dorland, 28th ed)
A phylum of anaerobic, gram-negative bacteria with a chemoorganotrophic heterotrophic metabolism. They are resident flora of the OROPHARYNX.
Large (>160 kDa) glycoproteins with electrophoretic mobility of ALPHA-GLOBULINS, found in high concentration in the plasma of pregnant women but also in a number of species including mouse, rat, and others (ALPHA-MACROGLOBULINS). In humans, they are always present in the circulation and are nonspecific inhibitors of PROTEINASES, similar to COMPLEMENT PROTEINS (C3; C4; C5).
Glycoproteins with a molecular weight of approximately 620,000 to 680,000. Precipitation by electrophoresis is in the alpha region. They include alpha 1-macroglobulins and alpha 2-macroglobulins. These proteins exhibit trypsin-, chymotrypsin-, thrombin-, and plasmin-binding activity and function as hormonal transporters.
A lymphoproliferative disorder characterized by pleomorphic B-LYMPHOCYTES including PLASMA CELLS, with increased levels of monoclonal serum IMMUNOGLOBULIN M. There is lymphoplasmacytic cells infiltration into bone marrow and often other tissues, also known as lymphoplasmacytic lymphoma. Clinical features include ANEMIA; HEMORRHAGES; and hyperviscosity.
Serum globulins that migrate to the gamma region (most positively charged) upon ELECTROPHORESIS. At one time, gamma-globulins came to be used as a synonym for immunoglobulins since most immunoglobulins are gamma globulins and conversely most gamma globulins are immunoglobulins. But since some immunoglobulins exhibit an alpha or beta electrophoretic mobility, that usage is in decline.
A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally being called a macroglobulin.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Plasma glycoprotein member of the serpin superfamily which inhibits TRYPSIN; NEUTROPHIL ELASTASE; and other PROTEOLYTIC ENZYMES.
A LDL-receptor related protein involved in clearance of chylomicron remnants and of activated ALPHA-MACROGLOBULINS from plasma.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Antibodies produced by a single clone of cells.
A glycoprotein that is central in both the classical and the alternative pathway of COMPLEMENT ACTIVATION. C3 can be cleaved into COMPLEMENT C3A and COMPLEMENT C3B, spontaneously at low level or by C3 CONVERTASE at high level. The smaller fragment C3a is an ANAPHYLATOXIN and mediator of local inflammatory process. The larger fragment C3b binds with C3 convertase to form C5 convertase.
A glycoprotein that is important in the activation of CLASSICAL COMPLEMENT PATHWAY. C4 is cleaved by the activated COMPLEMENT C1S into COMPLEMENT C4A and COMPLEMENT C4B.
The smaller fragment formed when complement C4 is cleaved by COMPLEMENT C1S. It is an anaphylatoxin that causes symptoms of immediate hypersensitivity (HYPERSENSITIVITY, IMMEDIATE) but its activity is weaker than that of COMPLEMENT C3A or COMPLEMENT C5A.
The smaller fragment generated from the cleavage of complement C3 by C3 CONVERTASE. C3a, a 77-amino acid peptide, is a mediator of local inflammatory process. It induces smooth MUSCLE CONTRACTION, and HISTAMINE RELEASE from MAST CELLS and LEUKOCYTES. C3a is considered an anaphylatoxin along with COMPLEMENT C4A; COMPLEMENT C5A; and COMPLEMENT C5A, DES-ARGININE.
A subcomponent of complement C1, composed of six copies of three polypeptide chains (A, B, and C), each encoded by a separate gene (C1QA; C1QB; C1QC). This complex is arranged in nine subunits (six disulfide-linked dimers of A and B, and three disulfide-linked homodimers of C). C1q has binding sites for antibodies (the heavy chain of IMMUNOGLOBULIN G or IMMUNOGLOBULIN M). The interaction of C1q and immunoglobulin activates the two proenzymes COMPLEMENT C1R and COMPLEMENT C1S, thus initiating the cascade of COMPLEMENT ACTIVATION via the CLASSICAL COMPLEMENT PATHWAY.
The minor fragment formed when C5 convertase cleaves C5 into C5a and COMPLEMENT C5B. C5a is a 74-amino-acid glycopeptide with a carboxy-terminal ARGININE that is crucial for its spasmogenic activity. Of all the complement-derived anaphylatoxins, C5a is the most potent in mediating immediate hypersensitivity (HYPERSENSITIVITY, IMMEDIATE), smooth MUSCLE CONTRACTION; HISTAMINE RELEASE; and migration of LEUKOCYTES to site of INFLAMMATION.
A fibrous protein complex that consists of proteins folded into a specific cross beta-pleated sheet structure. This fibrillar structure has been found as an alternative folding pattern for a variety of functional proteins. Deposits of amyloid in the form of AMYLOID PLAQUES are associated with a variety of degenerative diseases. The amyloid structure has also been found in a number of functional proteins that are unrelated to disease.
A highly conserved heterodimeric glycoprotein that is differentially expressed during many severe physiological disturbance states such as CANCER; APOPTOSIS; and various NEUROLOGICAL DISORDERS. Clusterin is ubiquitously expressed and appears to function as a secreted MOLECULAR CHAPERONE.
A family of cellular proteins that mediate the correct assembly or disassembly of polypeptides and their associated ligands. Although they take part in the assembly process, molecular chaperones are not components of the final structures.
A thrombin receptor subtype that couples to HETEROTRIMERIC GTP-BINDING PROTEINS resulting in the activation of a variety of signaling mechanisms including decreased intracellular CYCLIC AMP, increased TYPE C PHOSPHOLIPASES and increased PHOSPHOLIPASE A2.
A G-protein-coupled, proteinase-activated receptor that is expressed in a variety of tissues including ENDOTHELIUM; LEUKOCYTES; and the GASTROINTESTINAL TRACT. The receptor is activated by TRYPSIN, which cleaves off the N-terminal peptide from the receptor. The new N-terminal peptide is a cryptic ligand for the receptor. The uncleaved receptor can also be activated by the N-terminal peptide present on the activated THROMBIN RECEPTOR and by small synthetic peptides that contain the unmasked N-terminal sequence.
A group of sporadic, familial and/or inherited, degenerative, and infectious disease processes, linked by the common theme of abnormal protein folding and deposition of AMYLOID. As the amyloid deposits enlarge they displace normal tissue structures, causing disruption of function. Various signs and symptoms depend on the location and size of the deposits.
Exclusive legal rights or privileges applied to inventions, plants, etc.
A novel composition, device, or process, independently conceived de novo or derived from a pre-existing model.
Property, such as patents, trademarks, and copyright, that results from creative effort. The Patent and Copyright Clause (Art. 1, Sec. 8, cl. 8) of the United States Constitution provides for promoting the progress of science and useful arts by securing for limited times to authors and inventors, the exclusive right to their respective writings and discoveries. (From Black's Law Dictionary, 5th ed, p1014)
Time period from 1701 through 1800 of the common era.
Body of knowledge related to the use of organisms, cells or cell-derived constituents for the purpose of developing products which are technically, scientifically and clinically useful. Alteration of biologic function at the molecular level (i.e., GENETIC ENGINEERING) is a central focus; laboratory methods used include TRANSFECTION and CLONING technologies, sequence and structure analysis algorithms, computer databases, and gene and protein structure function analysis and prediction.
Time period from 1601 through 1700 of the common era.

Structural details of proteinase entrapment by human alpha2-macroglobulin emerge from three-dimensional reconstructions of Fab labeled native, half-transformed, and transformed molecules. (1/1037)

Three-dimensional electron microscopy reconstructions of native, half-transformed, and transformed alpha2-macroglobulins (alpha2Ms) labeled with a monoclonal Fab Fab offer new insight into the mechanism of its proteinase entrapment. Each alpha2M binds four Fabs, two at either end of its dimeric protomers approximately 145 A apart. In the native structure, the epitopes are near the base of its two chisel-like features, laterally separated by 120 A, whereas in the methylamine-transformed alpha2M, the epitopes are at the base of its four arms, laterally separated by 160 A. Upon thiol ester cleavage, the chisels on the native alpha2M appear to split with a separation and rotation to give the four arm-like extensions on transformed alpha2M. Thus, the receptor binding domains previously enclosed within the chisels are exposed. The labeled structures further indicate that the two protomeric strands that constitute the native and transformed molecules are related and reside one on each side of the major axes of these structures. The half-transformed structure shows that the two Fabs at one end of the molecule have an arrangement similar to those on the native alpha2M, whereas on its transformed end, they have rotated. The rotation is associated with a partial untwisting of the strands and an enlargement of the openings to the cavity. We propose that the enlarged openings permit the entrance of the proteinase. Then cleavage of the remaining bait domains by a second proteinase occurs with its entrance into the cavity. This is followed by a retwisting of the strands to encapsulate the proteinases and expose the receptor binding domains associated with the transformed alpha2M.  (+info)

Heme and acute inflammation role in vivo of heme in the hepatic expression of positive acute-phase reactants in rats. (2/1037)

Acute-phase protein synthesis in the liver during inflammation is regulated via cytokines and glucocorticoids. Using quantitative reverse transcription (RT)-PCR analysis and immunoassay, we explored, in the rat, the response of the acute-phase protein, alpha-2 macroglobulin (A2M), after systemic inflammation induced by lipopolysaccharide (LPS) or localized inflammation induced by turpentine oil (TO). The results indicate that synthesis of A2M is higher following TO-induced inflammation than LPS-induced inflammation and is not correlated with interleukin (IL)-6 or glucocorticoid levels. We studied the putative role of heme in this differential A2M expression following localized vs. systemic inflammation; addition of heme during LPS-induced inflammation can boost the expression of A2M, whereas blocking heme synthesis (by succinyl acetone) or enhancing its consumption in parallel biosynthetic pathways (cytochrome P450 induction by phenobarbital) decreases A2M expression. This decrease was abolished by exogenous heme supplementation. Finally, we demonstrate that heme supplementation is also able to increase the A2M response in female rats to a level similar to that in male rats providing a new insight into the puzzling sexual dimorphism observed previously during localized inflammation. We propose that heme should be considered a new regulatory element in controlling liver A2M expression during inflammation.  (+info)

Pig kidney legumain: an asparaginyl endopeptidase with restricted specificity. (3/1037)

Legumain was recently discovered as a lysosomal endopeptidase in mammals [Chen, Dando, Rawlings, Brown, Young, Stevens, Hewitt, Watts and Barrett (1997) J. Biol. Chem. 272, 8090-8098], having been known previously only from plants and invertebrates. It has been shown to play a key role in processing of the C fragment of tetanus toxin for presentation by the MHC class-II system [Manoury, Hewitt, Morrice, Dando, Barrett and Watts (1998) Nature (London) 396, 695-699]. We examine here the specificity of the enzyme from pig kidney by use of protein, oligopeptide and synthetic arylamide substrates, all determinations being made at pH 5.8. In proteins, only about one in ten of the asparaginyl bonds were hydrolysed, and these were mostly predicted to be located at turns on the protein surface. Bonds that were not cleaved in tetanus toxin were cleaved when presented in oligopeptides, sometimes faster than an equivalent oligopeptide based on a bond that was cleaved in the protein. Legumain cleaved the bait region of rat alpha1-macroglobulin and was 'trapped' by the macroglobulin, as most other endopeptidases are, but did not interact with human alpha2-macroglobulin, which contains no asparagine residue in its bait region. Glycosylation of asparagine totally prevented hydrolysis by legumain. Specificity for arylamide substrates was evaluated with reference to benzyloxycarbonyl-Ala-Ala-Asn-aminomethylcoumarin, and the preference for the P3-position amino acid was Ala>Tyr(tertiary butyl)>Val>Pro>Phe=Tyr>Leu=Gly. There was no hydrolysis of substrate analogues containing mono- or di-N-methylasparagines, l-2-amino-3-ureidopropionic acid or citrulline in the P1 position. We conclude that mammalian legumain appears to be totally restricted to the hydrolysis of asparaginyl bonds in substrates of all kinds. There seem to be no strong preferences for particular amino acids in other subsites, and yet there are still unidentified factors that prevent hydrolysis of many asparaginyl bonds in proteins.  (+info)

Alpha2-macroglobulin and eosinophil cationic protein in the allergic airway mucosa in seasonal allergic rhinitis. (4/1037)

As previously demonstrated in seasonal allergic rhinitis, increased microvascular permeability and eosinophil activation are key features of allergic airway inflammation. In the present study, the hypothesis that exudation of alpha2-macroglobulin may cause the appearance of eosinophil cationic protein (ECP) in the airway lumen was explored. Nasal lavages were carried out using the nasal pool device before and during the pollen season both at baseline and after histamine challenge in 10 children with allergic rhinitis. Nasal lavage fluid levels of alpha2-macroglobulin and ECP were determined. All patients experienced nasal symptoms of allergic rhinitis during the pollen season (p<0.01-0.05). Baseline nasal lavage fluid levels of alpha2-macroglobulin and ECP were increased during the season (p<0.01-0.05) and were found to be well correlated (p<0.0001). Histamine produced concentration-dependent plasma exudation before and during the pollen season, but it was only during the pollen season that this caused an increase in the lavage fluid levels of ECP (p<0.05). These data suggest that exudation of plasma and increased tissue levels and output of eosinophil cationic protein characterize nasal mucosal inflammation in children with seasonal allergic rhinitis. The plasma exudation process in part may account for lumenal entry of eosinophil cationic protein molecules that have been released in mucosal tissue compartments. A combination of induced exudation and nasal lavage may improve the yield of important markers of inflammation in studies of nasal diseases.  (+info)

NMR solution structure of complement-like repeat CR8 from the low density lipoprotein receptor-related protein. (5/1037)

The low density lipoprotein receptor-related protein is a member of the low density lipoprotein receptor family and contains clusters of cysteine-rich complement-like repeats of about 42 residues that are present in all members of this family of receptors. These clusters are thought to be the principal binding sites for protein ligands. We have expressed one complement-like repeat, CR8, from the cluster in lipoprotein receptor-related protein that binds certain proteinase inhibitor-proteinase complexes and used three-dimensional NMR on the 13C/15N-labeled protein to determine the structure in solution of the calcium-bound form. The structure is very similar in overall fold to repeat 5 from the low density lipoprotein receptor (LB5), with backbone root mean square deviation of 1.5 A. The calcium-binding site also appears to be homologous, with four carboxyl and two backbone carbonyl ligands. However, differences in primary structure are such that equivalent surfaces that might represent the binding interfaces are very different from one another, indicating that different domains will have very different ligand specificities.  (+info)

Abnormality in urinary protein excretion in Japanese men with impaired glucose tolerance. (6/1037)

OBJECTIVE: To examine whether subjects with impaired glucose tolerance (IGT) for more than 2 years have any abnormality in the kidney. RESEARCH DESIGN AND METHODS: We measured urinary excretion rate and clearance of various plasma proteins with different molecular radii and different isoelectric points in 22 Japanese men with IGT (IGT group) and 37 age-matched healthy control subjects (control group). RESULTS: Clearance of ceruloplasmin (molecular radius approximately 45 A; isoelectric point 4.4), IgG4 (molecular radius 55 A; isoelectric point 5.4), and IgG (molecular radius 55 A; isoelectric point 7.4) was significantly higher in the IGT group than in the control group, whereas there were no significant differences in urinary excretion rate of albumin (molecular radius 36 A; isoelectric points 4.8-5.2) and clearance of alpha 2-macroglobulin (molecular radius 88 A; isoelectric point 5.4) between the two groups. CONCLUSIONS: In the present study, we found that clearance of neutral-charged IgG, negatively charged IgG4, and ceruloplasmin with molecular radii of approximately 45-55 A was selectively increased in IGT subjects. This finding does not seem to be explained by impairment of charge and pore-size selectivity in the glomerulus. Therefore, considering the present result together with our recent finding that enhanced glomerular filtration rate (GFR) after acute protein loading in healthy subjects induced a selective increase in clearance of IgG, IgG4, and ceruloplasmin, we suggest that increased intraglomerular hydraulic pressure, although enhanced GFR was not demonstrated, may be at work in these mildly hyperglycemic subjects.  (+info)

Protein GRAB of streptococcus pyogenes regulates proteolysis at the bacterial surface by binding alpha2-macroglobulin. (7/1037)

In the molecular interplay between pathogenic microorganisms and their host, proteolytic mechanisms are believed to play a crucial role. Here we find that the important human pathogen Streptococcus pyogenes (group A Streptococcus) expresses a surface protein with high affinity (Ka = 2.0 x 10(8) M-1) for alpha2-macroglobulin (alpha2M), the dominating proteinase inhibitor of human plasma. The immunoglobulin-binding protein G of group C and G streptococci also contains an alpha2M-binding domain and a gene encoding protein GRAB (protein G-related alpha2M-binding protein) was identified in the S. pyogenes Genome Sequencing data base. The grab gene is present in most S. pyogenes strains and is well conserved. Protein GRAB has typical features of a surface-attached protein of Gram-positive bacteria. It also contains a region homologous to parts of the alpha2M-binding domain of protein G and a variable number of a unique 28-amino acid-long repeat. Using Escherichia coli-produced protein GRAB and synthetic GRAB peptides, the alpha2M-binding region was mapped to the NH2-terminal part of protein GRAB, which is the region with homology to protein G. An isogenic S. pyogenes mutant lacking surface-associated protein GRAB showed no alpha2M binding activity and was attenuated in virulence when injected intraperitoneally in mice. Finally, alpha2M bound to the bacterial surface via protein GRAB was found to entrap and inhibit the activity of both S. pyogenes and host proteinases, thereby protecting important virulence determinants from proteolytic degradation. This regulation of proteolytic activity at the bacterial surface should affect the host-microbe relation during S. pyogenes infections.  (+info)

Destruction of articular cartilage by alpha 2 macroglobulin elastase complexes: role in rheumatoid arthritis. (8/1037)

OBJECTIVE: Neutrophil elastase accounts for the ability of some fresh rheumatoid synovial fluids to degrade cartilage matrix in vitro. The aim of this study was to determine if enzyme activity could result from depletion of synovial fluid inhibitors or protection of the enzyme from inhibition. METHODS: The ability of synovial fluids to inhibit porcine pancreatic elastase was investigated together with chemical pretreatments capable of inactivating alpha 1 protease inhibitor (alpha 1PI) or preventing formation of alpha 2 macroglobulin (alpha 2M) elastase complexes. Subsequently, complexes of human neutrophil elastase with alpha 2M were prepared and applied to frozen sections of cartilage. Proteoglycan loss was quantified by alcian blue staining and scanning and integrating microdensitometry. Parallel studies were carried out using a low molecular weight chromogenic elastase substrate. The effects of alpha 1PI and SF on these systems were investigated. Finally, synovial fluids were subjected to gel filtration and the fractions assayed for elastase activity. High molecular weight fractions were pooled, concentrated, and tested for their ability to degrade cartilage sections. RESULTS: All synovial fluids reduced the activity of porcine pancreatic elastase, the inhibition mainly being attributable to alpha 1PI, whereas remaining activity resulted from complexes of elastase with alpha 2M. Complexes of human neutrophil elastase with alpha 2M were shown to cause proteoglycan degradation in frozen sections of human articular cartilage. Alpha 1PI prevented alpha 2M elastase complexes from degrading cartilage but not the chromogenic substrate. The data suggested that alpha 1PI does not inhibit elastase bound to alpha 2M but sterically hinders the complex. However, only one of five synovial fluids was able to completely block the actions of alpha 2M elastase complexes against cartilage. Gel filtration of rheumatoid synovial fluids showed elastase and cartilage degrading activity to be associated with fractions that contained alpha 2M, and not with fractions expected to contain free enzyme. CONCLUSIONS: The data suggest that synovial fluid alpha 2M elastase complexes can degrade cartilage matrix in rheumatoid arthritis.  (+info)

Rat alpha 1-macroglobulin (alpha 1M), rat alpha 2-macroglobulin (alpha 2M) migrated as single bands on non-denaturing gels when purified by the methods described. All three proteins demonstrated increased mobility after reaction with trypsin. A single saturable pathway rapidly cleared complexes of trypsin and the alpha-macroglobulins of mouse, rat and human from the circulation of mice. None of the native alpha-macroglobulins competed for clearance with the trypsin complexes. [14C]Methylamine incorporation was 4.1, 3.9, 2.6 and 3.2 mol/mol of proteinase inhibitor for human alpha 2M, rat alpha 1M, rat alpha 2M and mouse alpha 2M, respectively. Only rat alpha 2M, the acute-phase alpha-macroglobulin studied, showed no evidence of conformational change when subjected to electrophoresis after reaction with methylamine. The clearance of rat alpha 2M-methylamine was comparable with that of the native molecule. The other alpha-macroglobulin-methylamine complexes cleared faster than the inhibitors that ...
Glycoproteins with a molecular weight of approximately 620,000 to 680,000. Precipitation by electrophoresis is in the alpha region. They include alpha 1-macroglobulins and alpha 2-macroglobulins. These proteins exhibit trypsin-, chymotrypsin-, thrombin-, and plasmin-binding activity and function as hormonal transporters ...
Is able to inhibit all four classes of proteinases by a unique trapping mechanism. This protein has a peptide stretch, called the bait region which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase ...
The binding of 125I-labelled transforming growth factor-beta 1 (TGF-beta 1) to human alpha 2-macroglobulin (alpha 2M) was studied by native PAGE and autoradiography. TGF-beta 1 bound preferentially to alpha 2M-methylamine and minimally, if at all, to native alpha 2M. Preparations of alpha 2M-proteinase complex were generated by incubating a standard concentration of alpha 2M (0.4 microM) with different concentrations of trypsin, chymotrypsin or neutrophil elastase (0.04-2.0 microM). The 125I-TGF-beta 1-binding activity depended on the initial ratio of active proteinase to alpha 2M, or r value, used to form the alpha 2M-proteinase complex. With all three proteinases, r values of 2 or greater yielded preparations with unchanged or decreased TGF-beta 1-binding activity relative to native alpha 2M. By contrast, r values near 1 yielded preparations with significantly increased TGF-beta 1-binding activity. The results of [3H]thymidine-incorporation studies performed in mouse keratinocytes were ...
Extract: The present study using immunologic methodology confirms previous observations from this laboratory of an absence of a protease component with arginine esterase activity in plasma of patients with cystic fibrosis. In this study, the pooled plasma from control individuals was activated and partially purified after adsorption on columns of soybean trypsin inhibitor conjugated to Sepharose 4B followed by elution with benzamidine. The fraction was further purified by isoelectrofocusing on polyacrylamide gels. Proteins around the pI range of 5.5 were eluted and utilized to prepare an antiserum. Immunoelectrophoresis of activated plasma samples from control subjects and patients with cystic fibrosis was performed utilizing the antiserum. In controls, four precipitin arcs with residual esterase activity were observed, whereas only three were seen in plasma from patients with cystic fibrosis. Double gel diffusion experiments using specific antisera ruled out the presence of trypsin, chymotrypsin,
Is able to inhibit all four classes of proteinases by a unique trapping mechanism. This protein has a peptide stretch, called the bait region which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase (By similarity). Displays inhibitory activity against chymotrypsin, papain, thermolysin, subtilisin A and, to a lesser extent, elastase but not trypsin. May play an important role during desquamation by inhibiting extracellular proteases ...
Twenty-five monoclonal antibodies were generated by immunizing mice. Of these, the one named R10 had the strongest neutralizing activity against HAV [a 50% neutralizing concentration (neut50) value of ∼2 nM, ∼0.3 μg/mL]. The Fab fragment is also strongly neutralizing (neut50 = ∼3 nM, ∼0.45 μg/mL) (Fig. 1A). R10 does not recognize linear epitopes by immunoblot, but recognizes conformational epitopes by ELISA, and shows similar binding affinities to full and empty particles (Fig. 1B). Interestingly, a fluorescent assay revealed that whereas the R10 Fab portion destabilizes full HAV particles by 7 °C, the intact R10 stabilizes the particles by 2 °C (Fig. 1C and Fig. S1A). The same assay indicates that the bivalent intact antibody induces a two-stage transition in protein conformation to release the RNA genome (Fig. 1C and Fig. S1A). The first event, producing a slight exposure of RNA, occurs at a similar temperature to that seen with the Fab, whereas the second, which fully exposes the ...
Alpha-2-Macroglobulin (α2M) is a large (720 KDa) plasma protein found in the blood. It is mainly produced by the liver, and also locally synthesized by macrophages, fibroblasts, and adrenocortical cells. In humans it is encoded by the A2M gene. Alpha 2 macroglobulin acts as an antiprotease and is able to inactivate an enormous variety of proteinases. It functions as an inhibitor of fibrinolysis by inhibiting plasmin and kallikrein. It functions as an inhibitor of coagulation by inhibiting thrombin. Alpha-2-macroglobulin may act as a carrier protein because it also binds to numerous growth factors and cytokines, such as platelet-derived growth factor, basic fibroblast growth factor, TGF-β, insulin, and IL-1β. No specific deficiency with associated disease has been recognized, and no disease state is attributed to low concentrations of alpha-2-macroglobulin. The concentration of alpha-2-macroglobulin rises 10-fold or more in the nephrotic syndrome when other lower molecular weight proteins are ...
This is a domain of the alpha-2-macroglobulin family.. The alpha-macroglobulin (aM) family of proteins includes protease inhibitors [(PUBMED:2473064)], typified by the human tetrameric a2-macroglobulin (a2M); they belong to the MEROPS proteinase inhibitor family I39, clan IL. These protease inhibitors share several defining properties, which include (i) the ability to inhibit proteases from all catalytic classes, (ii) the presence of a bait region and a thiol ester, (iii) a similar protease inhibitory mechanism and (iv) the inactivation of the inhibitory capacity by reaction of the thiol ester with small primary amines. aM protease inhibitors inhibit by steric hindrance [(PUBMED:2472396)]. The mechanism involves protease cleavage of the bait region, a segment of the aM that is particularly susceptible to proteolytic cleavage, which initiates a conformational change such that the aM collapses about the protease. In the resulting aM-protease complex, the active site of the protease is sterically ...
The present invention relates to the use of alpha (2) macroglobulin complexes isolated from the serum of a mammal. The invention also relates to methods for making such complexes and compositions com
alpha 2 Macroglobulin antibody [N2N3] (alpha-2-macroglobulin) for WB. Anti-alpha 2 Macroglobulin pAb (GTX112856) is tested in Human samples. 100% Ab-Assurance.
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Dear visitor, welcome to FertilityZone . If this is your first visit here, please read the Help. It explains in detail how this page works. To use all features of this page, you should consider registering. Please use the registration form, to register here or read more information about the registration process. If you are already registered, please login here.. ...
Pregnant women beware - if you eat genetically modified organism or the GMO foods, you may be exposing your unborn baby to dangerous toxins.
BioAssay record AID 4853 submitted by ChEMBL: Compound was tested for its ability to displace [125I]GTI binding to 5-hydroxytryptamine 1D receptor recognition sites in pig caudate membranes.
Nonproteolytic incorporation of protein ligands into human alpha 2-macroglobulin: implications for the binding mechanism of alpha 2-macroglobulin.
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Complement C5 (C3 And PZP Like Alpha 2 Macroglobulin Domain Containing Protein 4 or C5) - Pipeline Review, H2 2017 Download the full report: https://www.
Content - SummaryComplement C5 (C3 And PZP Like Alpha 2 Macroglobulin Domain Containing Protein 4 or C5) pipeline Target constitutes close to 33 molec
Buy our Natural Human alpha 2 Macroglobulin protein. Ab91104 is a full length protein produced in Nativesyntheticaly and has been validated in SDS-PAGE. Abcam…
Provided are compounds of Formula I: Formula I or a pharmaceutically acceptable salt thereof, wherein R 2 , R 4 , R 5 , R 6 , R 7 , R 8 , R 9 , X, Z 1 , Z 2 , Z 3 , Z 4 and m are as defined herein. Also provided is a pharmaceutically acceptable composition comprising a compound of Formula I, or a pharmaceutically acceptable salt thereof. Also provided are methods of using a compound of Formula I, or a pharmaceutically acceptable salt thereof.
Please optimize the BrU concentraiton, pulse-time, or chase-time. BrU concentrations of more than 500 μM may affect cell growth and/or other biological processes ...
keywords = {*Gene Expression Regulation, *Genes, *Genome, *Homeostasis, *Immunity, *Models, *Nobel Prize, *RNA Interference, *RNA Interference, *Virulence, 5 Untranslated Regions, Abdomen, Actins, Active, Acute-Phase Proteins, Acute-Phase Proteins, Acute-Phase Proteins, Acute-Phase Proteins, Acylation, Adaptor Proteins, Adaptor Proteins, Adaptor Proteins, Adaptor Proteins, Adaptor Proteins, Adaptor Proteins, Adaptor Proteins, Adaptor Proteins, Adaptor Proteins, Adaptor Proteins, Adenosine Triphosphatases, Adenosine Triphosphate, Adenosine Triphosphate, Adipocytes, Adipogenesis, Adipose Tissue, Adipose Tissue, Adipose Tissue, Adult Stem Cells, Aedes, Aedes, Aedes, Aedes, Aedes, Aedes, Age Factors, Age Factors, Age Factors, Age Factors, Agglutination, Agglutination, Aging, Aging, Alleles, Alleles, Allergy and Immunology, alpha-Macroglobulins, alpha-Macroglobulins, alpha2-macroglobulins, Alphavirus, Alphavirus Infections, Alternative infection models, Alternative Splicing, Amino Acid, Amino Acid, ...
Synthetic peptides derived from the G-H loop of the Foot-and Mouth Disease Virus (FMDV) capsid protein VP1 are relatively poor at recapitulating the native conformation present in the virus, and thus are often poor immunogens. We hypothesized that a candidate mucosal vaccine against FMDV could be developed using the non-toxic Pseudomonas aeruginosa exotoxin A (ntPE) and B subunit of E. coli heat labile enterotoxin (LTB) to deliver the G-H loop in its native conformation. PE, secreted by Pseudomonas aeruginosa is a mucosal adjuvant that binds to α2-macroglobulin receptor/Low density Lipoprotein receptor-related Protein (LRP1)/CD91 present on epithelial cells and APC plasma membranes to traverse the mucosal barrier. The LTB subunit, a 55KDa pentameric molecule is also a potent mucosal adjuvant and is responsible for binding to various eukaryotic cell receptors including GM1, glycosphingolipids, glycoprotein receptors, polyglycosilceramides and paraglobosides. ^ The chimeric protein ntPE-GH was
The present invention relates to the use of alpha (2) macroglobulin complexes isolated from the serum of a mammal. The invention also relates to methods for making such complexes and compositions comprising alpha (2) macroglobulin complexes, isolated from the serum of a mammal, wherein such compositions are used in methods for the treatment and prevention of cancer and infectious disease. The invention also relates to methods for treating and preventing cancer and infectious disease using such complexes comprising, isolated from the serum of a mammal. The invention also encompasses methods for production of alpha (2) macroglobulin complexes.
Hep I stimulates association of LRP with the Gαi2 subunit, and association is blocked by RAP and by peptides corresponding to the COOH terminus of Gαi2. (A) BAE cells were grown to near confluence and serum deprived overnight. Cells were then treated with 1 μM hep I for 0, 5, 10, 15, or 30 min and lysed. Cell lysates were immunoprecipitated with monoclonal anti-LRP antibody (8G1), separated by SDS-PAGE, and immunoblotted with mouse anti-Gαi2 antibodies. A representative immunoblot for LRP-associated Gαi2 is shown. Bands were analyzed using One-Dscan software (Scanalytics), and the fold change in LRP-Gαi2 association as compared with untreated conditions was determined. Equal loading of sample protein was assessed by protein staining with Ponceau S. *, P , 0.05; **, P , 0.01 (n = 3-5). (B) BAE cells were grown as in A, treated for 10 min with either DMEM, 100 nM hep I, 78 nM TSP, or 100 nM modified hep I and assayed for LRP-Gαi2 association as described in A. A representative immunoblot ...
Disease-causing bacteria in the lung evade the bodys immune system by pretending the lungs are pregnant, according to University of Dundee research presented today at the European Respiratory Society Congress in Madrid.. A new study, funded by the British Lung Foundation and the Scottish Governments Chief Scientist Office, has revealed that people with two common lung diseases - COPD and bronchiectasis - produce Pregnancy Zone Protein (PZP) in their lungs when they have a chest infection.. For the full article, please see here:. https://www.dundee.ac.uk/news/2019/lung-bacteria-feign-pregnancy-to-avoid-detection.php. ...
phdthesis{e0687830-50ba-47d3-97d5-1bd517693461, abstract = {Cell wall-attached proteins (CWPs) are important for the virulence of Gram-positive bacteria, but are also targets for the host immune system. CWPs have a conserved COOH-terminal region, responsible for cell wall-sorting. A pattern based on this conserved region was developed. The pattern identifies genes encoding CWPs, with high sensitivity and specificity, in whole genomes of Gram-positive bacteria. In genomes of five Gram-positive bacterial species, 19 previously unknown putative CWPs were identified.,br/,,br, ,br/,,br, Three novel CWPs were identified and characterised in <i>Streptococcus pyogenes</i>, an important Gram-positive human pathogen. One CWP, GRAB, binds the human proteinase inhibitor alpha<sub>2</sub>-macroglobulin to the <i>S. pyogenes</i> surface, thus protecting other CWPs from proteolysis. Moreover, two novel CWPs (SclA and SclB) with regions similar to collagen were studied. These ...
Ulery PG, Beers J, Mikhailenko I, Tanzi RE, Rebeck GW, Hyman BT, Strickland DK. Modulation of beta-amyloid precursor protein processing by the low density lipoprotein receptor-related protein (LRP). Evidence that LRP contributes to the pathogenesis of Alzheimers disease ...
pep:known chromosome:VEGA66:19:3584828:3686556:-1 gene:OTTMUSG00000042195 transcript:OTTMUST00000110769 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Lrp5 description:low density lipoprotein receptor-related protein 5 ...
Using cryo-electron microscopy, an international group of scientists have reported a potent HAV-specific neutralizing monoclonal antibody R10 that blocks receptor attachment and interferes viral uncoating. High resolution cryo-EM structures of HAV full and empty particles and of the complex of HAV with R10 Fab reveal the atomic details of antibody binding and point to a receptor recognition site at the pentamer interface. HAV is unique amongst picornaviruses in targeting the liver, and despite a successful vaccine continues to be a source of mortality. It has radically different properties from other picornaviruses, for instance it exists in an enveloped form and is unusually stable, both genetically and physically. In 2015, IBP scientists solved crystal structure of unenveloped HAV showing no trace of the canyon which encircles the 5-fold axes of enteroviruses and is often the site of receptor binding. However, the molecular basis where the cellular receptor TIM-1 might attach remains unknown. ...
Gentaur molecular products has all kinds of products like :search , Accu \ Alpha_2_Macroglobulin, Goat anti_Human, Rabbit, Bovine, Swine, Dog, Cat \ ACL20010AP for more molecular products just contact us
V / __/ , , ,_, ,_,\__,_,_, \_/ \___,_,_, _ _ ____ _ , , , , , __ ) ___ ___ , ,_ , , , ,___, _ \ / _ \ / _ \, __, , ,_, ,___, ,_) , (_) , (_) , ,_ \___/ ,____/ \___/ \___/ \__, ** MARVELL BOARD: DB-88F6282A-BP LE U-Boot 1.1.4 (Feb 22 2011 - 14:06:09) Marvell version: 3.5.9 - Cisco Plasma U-Boot code: 00600000 -, 0067FFF0 BSS: -, 006D2340 Soc: 88F6282 A1CPU running @ 1600Mhz L2 running @ 533Mhz SysClock = 400Mhz , TClock = 200Mhz DRAM (DDR3) CAS Latency = 6 tRP = 6 tRAS = 15 tRCD=6 DRAM CS[0] base 0x00000000 size 256MB DRAM CS[1] base 0x10000000 size 256MB DRAM Total size 512MB 16bit width Addresses 8M - 0M are saved for the U-Boot usage. Mem malloc Initialization (8M - 7M): Done NAND:512 MB Flash: 0 kB CPU : Marvell Feroceon (Rev 1) Streaming disabled Write allocate disabled Module 0 is RGMII USB 0: host mode PEX 0: interface detected no Link. PEX 1: interface detected no Link. Net: egiga0 [PRIME], egiga1 Hit any key to stop autoboot: 3 2 1 0 ### JFFS2 loading boot.img to 0x400000 Scanning ...
You need to find all english words ending in M ? We have that list and for sure you will find the word you are looking for. All these words ending in M are sorted by the number of points they give at Scrabble.
Fórum. Old Punx fest - letní punkový open air festival se zcela novou a originální koncepcí. Vystoupí zde pouze legendy - kapely, které jsou na scéně déle než 18 let. To vše v novém areálu Milín..
Fórum. Old Punx fest - letní punkový open air festival se zcela novou a originální koncepcí. Vystoupí zde pouze legendy - kapely, které jsou na scéně déle než 18 let. To vše v novém areálu Milín..
where n and m are just integers. These kind of integrals come up all the time in vector calculus and they take me ages to do. Is there a general method of doing them or a few common integrals i could learn? I end up having to apply about 3 trig identites and then sorting out the mess of resulting sinxs, cosx, cos2x ...
RESULTS The top two urine proteins with the highest difference in ratio of case subjects to control subjects were haptoglobin (8.7 times; P , 0.0001) and α-2-macroglobulin (5.7 times; P , 0.0001). In the cohort study, patients with baseline urinary haptoglobin ≥20 ng/min (haptoglobinuria) had a higher incidence of CRI than those without (hazard ratio [95% CI] 3.27 [1.41-7.58]; P = 0.006). The overall CRI rate was 3.2% for patients without haptoglobinuria or MAU, 9.5% for those with MAU, and 13.3% for those with haptoglobinuria. The highest rate for CRI (22.4%) was in patients with both MAU and haptoglobinuria (P , 0.001). ...
In the measurement of plasmin inhibitor three reactions of plasmin are of importance. First is the reaction with the PB-form of the plasmin inhibitor. Second is the reaction with the NPB-form of the plasmin inhibitor and third the reaction with a 2 -macroglobulin. Using a small excess of plasmin ( approx. 1 m M), all three reactions take place as a second order reaction ([plasmin inhibitor] total approx. [plasmin] total ). At a plasmin inhibitor concentration of 1 m M gives this a velocity for the plasmin-PB-plasmin inhibitor complex formation of 27 s -1 . For the plasmin-NPB-plasmin inhibitor complex formation this is 0.2 s -1 and for the plasmin- a 2 -macroglobulin complex formation this is 1.0 s -1 . Expressed in ratios of the complexes this is 95.7% for the plasmin-PB-plasmin inhibitor complex, 0.7% for the plasmin-NPB-plasmin inhibitor complex and 3.6% for the plasmin- a 2 -macroglobulin complex ...
Gelatinase B/matrix metalloproteinase-9 (MMP-9), a key regulator and effector of immunity, contains a C-terminal hemopexin domain preceded by a unique linker sequence of ?64 amino acid residues. This linker sequence is demonstrated to be an extensively O-glycosylated (OG) domain with a compact three-dimensional structure. The OG and hemopexin domains have no influence on the cleavage efficiency of MMP-9 substrates. In contrast, the hemopexin domain contains a binding site for the cargo receptor low density lipoprotein receptor-related protein-1 (LRP-1). Furthermore, megalin/LRP-2 is identified as a new functional receptor for the hemopexin domain of MMP-9, able to mediate the endocytosis and catabolism of the enzyme. The OG domain is required to correctly orient the hemopexin domain for inhibition by TIMP-1 and internalization by LRP-1 and megalin. Therefore, the OG and hemopexin domains down-regulate the bioavailability of active MMP-9 and the interactions with the cargo receptors are proposed ...
LRP5 - LRP5 (GFP-tagged) - Human low density lipoprotein receptor-related protein 5 (LRP5) available for purchase from OriGene - Your Gene Company.
Inhibition of AB5-type bacterial toxins can be achieved by heterobifunctional ligands (BAITs) that mediate assembly of supramolecular complexes involving the toxins pentameric cell membrane-binding subunit and an endogenous protein, serum amyloid P component, of the innate immune system. Effective in vivo protection from Shiga toxin Type 1 (Stx1) is achieved by polymer-bound, heterobifunctional inhibitors-adaptors (PolyBAITs), which exhibit prolonged half-life in circulation and by mediating formation of face-to-face SAP-AB5 complexes, block receptor recognition sites and redirect toxins to the spleen and liver for degradation. Direct correlation between solid-phase activity and protective dose of PolyBAITs both in the cytotoxicity assay and in vivo indicate that the mechanism of protection from intoxication is inhibition of toxin binding to the host cell membrane. The polymeric scaffold influences the activity not only by clustering active binding fragments but also by sterically interfering with the
Fibrinogen γ-chain dodecapeptide, 5 mg. The synthetic dodecapeptide HHLGGAKQAGDV represents the specific platelet receptor recognition site of the human fibrinogen �� chain (residues 400-411).
Abstract. Intrahepatic transplantation of islets requires a lot of islets because more than 50% of the graft is lost during the 24 hours following transplantation. We analyzed, in a rat model, early post-transplantation inflammation using systemic inflammatory markers, or directly in islet-transplanted livers by immunohistochemistry. 1H HRMAS NMR was employed to investigate metabolic responses associated with the transplantation. Inflammatory markers (Interleukin-6, α2-macroglobulin) are not suitable to follow islet reactions as they are not islet specific. To study islet specific inflammatory events, immunohistochemistry was performed on sections of islet transplanted livers for thrombin (indicator of the instant blood-mediated inflammatory reaction (IBMIR)) and granulocytes and macrophages. We observed a specific correlation between IBMIR and granulocyte and macrophage infiltration after 12 h. In parallel, we identified a metabolic response associated with transplantation: after 12 h, ...
Meeting planners find Panza dIschia meeting hotel information for your next Panza dIschia meeting, conference, or event. Meeting room information for Panza dIschia hotels with meeting facilities. Find a meeting hotel in Panza dIschia by room amenities, services, size, price and location. Find convention centers and meeting locations by available resources and specifications
Pulmonary tuberculosis (PTB) is global disease affecting about one third of the worlds population with its attendant mortality and morbidity. Acute phase proteins have been used in monitoring the progression of infections but not in relation to PTB in this environment. The levels of total protein, albumin, α-2-macroglobulin, transferrin, and haptoglobulin were determined in 23 patients with PTB and 17- age / sex matched PTB-free controls using spectrophotometric and immunodiffusion methods respectively. The result showed that α-2-macroglobulin was significantly raised in PTB patients compared with controls ( ...
1 front of precipitation against rabbit antisera to halpha2-M. Scientific note: Serum glycoprotein of MW approx. 800,000 daltons, containing 38 histidine residues. Two protomers of MW aprox. 370,000 daltons. Each protomer is formed by two sub-units of MW 185,000 daltons, linked by disulfide bridges. N-linked complex carbohydrate side chains ...
Looking anywhere north of the eastern but south of the ring road - pretty flexible!. Me (24, F) and my dog Tobias (1, M) are on the hunt for a queen size room with my own bathroom (preferably, but Im flexible on this) in a pet friendly house that has a bit of a backyard. Weve given our 4 weeks notice at our current place, so wanting to move before the end of Feb. More than happy to join a lease, or start a new one! Budget up to $200 a week or $240 for my own bathroom excl bills. To save you reading the novel below, both me and Toby are clean, friendly and respectful and enjoy watching sports over a bev! We have two solid incomes between us (both from me) and Toby will definitely keep you entertained with his antics!. About me ...
Quantity100 testsVolume2.0 mLImmunogenHuman granulocytesBackground InformationCD11b (integrin αM subunit) is a 165-170 kDa type I transmembrane glyco…
1989). "A cluster of alpha 2-macroglobulin-related genes (alpha 2 M) on human chromosome 12p: cloning of the pregnancy-zone ... "Proteolytic hydrolysis and purification of the LRP/alfa-2-macroglobulin receptor domain from alpha-macroglobulins". Protein ... Comparison with human alpha 2-macroglobulin". J. Biol. Chem. 260 (29): 15723-35. PMID 2415522. Sottrup-Jensen L, Birkedal- ... Genomic structure of the bait domains of human pregnancy zone protein and alpha 2 macroglobulin". FEBS Lett. 262 (2): 349-52. ...
Alpha-2-macroglobulin-like 1 abbreviated as α2ML1 is a protein that in humans is encoded by the A2ML1 gene. α2ML1 is a large, ... This gene encodes a member of the alpha-macroglobulin superfamily. The encoded protein acts as an inhibitor for several ... Alpha-2-macroglobulin-like 1". Galliano MF, Toulza E, Gallinaro H, Jonca N, Ishida-Yamamoto A, Serre G, Guerrin M (November ... "The protease inhibitor alpha-2-macroglobulin-like-1 is the p170 antigen recognized by paraneoplastic pemphigus autoantibodies ...
Alpha 2-antiplasmin and alpha 2-macroglobulin inactivate plasmin. Plasmin activity is also reduced by thrombin-activatable ... Such results can be seen in peoples with liver disease, PAI-1 deficiency or alpha 2-antiplasmin deficiency. Similar results are ... Nevertheless, excess levels of PAI and alpha 2-antiplasmin have been implicated in the metabolic syndrome and various other ... alpha 2-antiplasmin, and plasminogen) from plasma and then observing the time required for clot dissolution. A shortened lysis ...
It is a glycoprotein that binds to the alpha-2-macroglobulin receptor, as well as to other members of the low density ... Jou YS, Goold RD, Myers RM (Nov 1994). "Localization of the alpha 2-macroglobulin receptor-associated protein 1 gene (LRPAP1) ... coding for the alpha 2-macroglobulin receptor-associated protein and structural comparison with the murine gene coding for the ... "Primary structure of alpha 2-macroglobulin receptor-associated protein. Human homologue of a Heymann nephritis antigen". The ...
Comparison to the TGF-beta-alpha 2-macroglobulin interaction". Eur. J. Biochem. 221 (2): 687-93. doi:10.1111/j.1432-1033.1994. ...
Alpha 2-macroglobulin and coagulation factors affect coagulation, mainly stimulating it. This pro-coagulant effect may limit ... 1993). "Alpha-2-macroglobulin functions as an inhibitor of fibrinolytic, clotting, and neutrophilic proteinases in sepsis: ...
alpha-2-Macroglobulin (A2M): is also a major inhibitor of thrombin. It is present in 2-3 fold higher concentration during ... the functional role of alpha-2-macroglobulin and influence of Developmental Haemostasis. British Journal of Haematology, 138(3 ...
"Gene expression of the high molecular weight proteinase inhibitor alpha 2-macroglobulin". Biol. Chem. Hoppe-Seyler. 373 (7): ... Kluck PM, Wiegant J, Jansen RP, Bolk MW, Raap AK, Willemze R, Landegent JE (1993). "The human interleukin-6 receptor alpha ... The interleukin-6 receptor can serve as an alpha-receptor for CTNF". J. Biol. Chem. 278 (11): 9528-35. doi:10.1074/jbc. ...
ApoER2 splice variants can act as a receptor for alpha-2-macroglobulin which can have a role in clearance of alpha-2- ... which is controlled by proteolytic inhibitors such as alpha-2-macroglobulin. Studies have shown that a high presence of alpha-2 ... Isolation of cDNA encoding proteins associated with Aβ was used to discover alpha-2-macroglobulin. These discoveries may link ... alpha-2-macroglobulin and its receptors, one of them being ApoER2, to Alzheimer's disease. ApoER2 interaction with reelin and ...
Cleaves synthetic substrates and alpha-macroglobulins at bonds where P1' is a hydrophobic residue This enzyme belongs to the ... Localization of cleavage sites in the bait regions of five mammalian α-macroglobulins". J. Biol. Chem. 264: 393-401. PMID ... Sottrup-Jensen, L.; Birkedal-Hansen, H. (1989). "Human fibroblast collagenase-α-macroglobulin interactions. ...
... synergy with GR for full transcriptional increase of the alpha 2-macroglobulin gene". Genes Dev. 17 (20): 2564-77. doi:10.1101/ ... The role of alpha and beta isoforms of the glucocorticoid receptor]". Arch. Bronconeumol. 38 (9): 436-40. PMID 12237016. Bray ...
... synergy with GR for full transcriptional increase of the alpha 2-macroglobulin gene". Genes & Development. 17 (20): 2564-77. ... Kawasaki A, Matsumura I, Kataoka Y, Takigawa E, Nakajima K, Kanakura Y (May 2003). "Opposing effects of PML and PML/RAR alpha ... "Involvement of tyrosine phosphatase PTP1D in the inhibition of interleukin-6-induced Stat3 signaling by alpha-thrombin". ...
... such as alpha-2 macroglobulin and lipoproteins. An increase in the latter can cause the hyperlipidemia associated with this ...
Low density lipoprotein receptor-related protein 1 (LRP1), also known as alpha-2-macroglobulin receptor (A2MR), apolipoprotein ... "The low density lipoprotein receptor-related protein/alpha 2-macroglobulin receptor binds and mediates catabolism of bovine ... The cytoplasmic domain of LRP1 is the alpha-chain, which comprises four ligand-binding domains (numbered I-IV) containing two, ... LRP1 has been shown to interact with: A2-Macroglobulin, β-amyloid precursor protein, APBB1, APOE, Aprotinin, C1S/C1q inhibitor ...
"The low density lipoprotein receptor-related protein/alpha 2-macroglobulin receptor binds and mediates catabolism of bovine ... domain of lipoprotein lipase binds to the low density lipoprotein receptor-related protein/alpha 2-macroglobulin receptor (LRP ...
Anti-inflammatory property of withaferin A has been attributed to its ability to suppress alpha-2 macroglobulin, NF-κB and AP1 ... It binds to intermediate filament protein, vimentin by covalently modifying its highly conserved cysteine residue in alpha- ...
... elafin and alpha-2-Macroglobulin-like 1. Both Zn2+ and Cu2+ ions are also able to inhibit KLK7. KLK7 is a chymotrypsin-like ... "A novel protease inhibitor of the alpha2-macroglobulin family expressed in the human epidermis". J Biol Chem. 281 (9): 5780- ...
Interaction of alpha-2-Macroglobulin and HSV-1 during infection of neuronal cells». Journal of Neurovirology., 7: 556-63. Bello ... connection with the tumor necrosis factor alpha signalling pathway». Journal of Virology, 72: 8893-8903. López-Guerrero, J. A ...
2002). "Cell surface antigen CD109 is a novel member of the alpha(2) macroglobulin/C3, C4, C5 family of thioester-containing ... 2004). "CD109 represents a novel branch of the alpha2-macroglobulin/complement gene family". Gene. 327 (2): 171-83. doi:10.1016 ...
... and alpha-2-macroglobulin. This data is based on affinity capture- mass spectrometry. POTEB expression is low or completely ... However, there is unpublished data suggesting an interaction between POTEB and alpha-1-B glycoproteins, APOBEC1 complementation ...
Alpha 1-antitrypsin and alpha-2-macroglobulin are human serum protease inhibitors that completely inhibit the general ...
... alpha 1-antichymotrypsin MeSH D12.776.377.715.085.085 - alpha 1-antitrypsin MeSH D12.776.377.715.085.100 - alpha-macroglobulins ... alpha-macroglobulins MeSH D12.776.402.150.100.200 - chimerin 1 MeSH D12.776.402.150.500.460 - neurofibromin 1 MeSH D12.776. ... alpha-crystallin a chain MeSH D12.776.306.366.100.300 - alpha-crystallin b chain MeSH D12.776.306.366.300.100 - beta-crystallin ... steroid 12-alpha-hydroxylase MeSH D12.776.422.220.453.915.737 - steroid 16-alpha-hydroxylase MeSH D12.776.422.220.453.915.748 ...
... alpha-macroglobulins MeSH D12.776.124.790.720.100.500 -- pregnancy-associated alpha 2-macroglobulins. ... alpha 1-antitrypsin MeSH D12.776.124.790.106.100 -- alpha-macroglobulins MeSH D12.776.124.790.106.118 -- antiplasmin MeSH ... immunoglobulin alpha-chains MeSH D12.776.124.486.485.114.619.251 -- immunoglobulin d MeSH D12.776.124.486.485.114.619.251.500 ... immunoglobulin alpha-chains MeSH D12.776.124.486.485.705.500.360 -- immunoglobulin delta-chains MeSH D12.776.124.486.485.705. ...
... inhibitor type 1 are internalized by means of the low density lipoprotein receptor-related protein/alpha 2-macroglobulin ... Tissue plasminogen activator has been shown to interact with: Fibrinogen alpha chain LRP1 SERPINI1 tPA and plasmin are the key ... alpha C-domains". Biochemistry. 40 (3): 801-8. doi:10.1021/bi001789t. PMID 11170397. Ichinose A, Takio K, Fujikawa K (1986). " ...
... alpha 2-macroglobulin, alpha 1-antitrypsin, transferrin, and plasminogen.[citation needed] They have been grown successfully in ...
Alpha-2-macroglobulin, Haptoglobin, Apolipoprotein A1, Gamma-glutamyl transpeptidase (GGT), Total bilirubin, and Alanine ... FibroSure uses quantitative results of five serum biochemical markers, α2-macroglobulin, haptoglobin, apolipoprotein A1, ...
... alpha-2-Macroglobulin Pefabloc SC Pepstatin PMSF (phenylmethanesulfonyl fluoride) TLCK Trypsin inhibitors Kunitz domain ... The propeptide region has an open-sandwich antiparallel-alpha/antiparallel-beta fold, with two alpha-helices and four beta- ... It forms an alpha-helical domain that runs through the substrate-binding site, preventing access. Removal of this region by ... In medicine, protease inhibitor is often used interchangeably with alpha 1-antitrypsin (A1AT, which is abbreviated PI for this ...
... alpha-2-macroglobulin, von Willebrand factor, LDL cholesterol and immunoglobulin M. It is an experimental treatment for dry age ...
... alpha-1-glicoproteine, alpha 1 antitrypsin, alpha-2-macroglobulin, transferrin, globulin tyrosine, and hormonal systems are ... "Can the clearance of tumor necrosis factor alpha and interleukin 6 be enhanced using an albumin dialysate hemodiafiltration ...
... alpha-1 antitrypsin and alpha-2-macroglobulin, which inhibit the naturally occurring fibrinolytic agent, plasmin. 2) The blood ...
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Alpha 1-antichymotrypsin. *Alpha 1-antitrypsin. *Alpha 2-macroglobulin. *C-reactive protein ... Alpha globulins. .mw-parser-output .nobold{font-weight:normal}. serpins:. *alpha-1 (Alpha 1-antichymotrypsin, Alpha 1- ... The protein is composed of alpha helices and beta sheets that form two domains.[8] The N- and C- terminal sequences are ...
α2 - haptoglobin, α2-macroglobulin, α2-antiplasmin, ceruloplasmin. The beta (β) band - transferrin, LDL, complement The gamma ( ... The globulins are classified by their banding pattern (with their main representatives): The alpha (α) band consists of two ...
alpha-2 (Ceruloplasmin). *Retinol binding protein. other:. *alpha-1 (Orosomucoid). *alpha-2 (alpha-2-Macroglobulin, Haptoglobin ... Feldmann M, Maini R (2001). "Anti-TNF alpha therapy of rheumatoid arthritis: what have we learned?". Annu Rev Immunol. 19 (1): ... Possible classes of heavy chains in antibodies include alpha, gamma, delta, epsilon, and mu, and they define the antibody's ... Streptococcal IgA1 protease, digestion, Fab and Fc fragments, and the complete amino acid sequence of the alpha 1 heavy chain ...
platelet alpha granule membrane. • integrin alphav-beta3 complex. • extracellular exosome. • lamellipodium membrane. • cell ... Sajid M, Stouffer GA (2002). "The role of alpha(v)beta3 integrins in vascular healing". Thromb. Haemost. 87 (2): 187-93. doi: ... The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha ... Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as ...
Lectins, such as concanavalin A are proteins which can bind specific alpha-D-mannose and alpha-D-glucose carbohydrate molecules ... Of many uses of affinity chromatography, one use of it is seen in affinity purification of albumin and macroglobulin ... This type of purification is helpful in removing excess albumin and α2-macroglobulin contamination, when performing mass ...
Like other serpins, PAI-2 has three beta sheets (A, B, C) and nine alpha helices (hA-hI).[6][7] The structure of PAI-2 mutants ... gene are associated with basal and tumor necrosis factor-alpha-induced transcription in monocytes". European Journal of ... "Plasminogen activator inhibitor type 2 inhibits tumor necrosis factor alpha-induced apoptosis. Evidence for an alternate ...
alpha-2 (Ceruloplasmin). *Retinol binding protein. other:. *alpha-1 (Orosomucoid). *alpha-2 (alpha-2-Macroglobulin, Haptoglobin ... Alpha-fetoprotein (alpha-fetoglobulin) is a fetal plasma protein that binds various cations, fatty acids and bilirubin. Vitamin ... A number of blood transport proteins are evolutionarily related, including serum albumin, alpha-fetoprotein, vitamin D-binding ... alpha-fetoprotein, and vitamin D-binding protein gene family". The Journal of Biological Chemistry. 269 (27): 18149-54. PMID ...
alpha-2 (Ceruloplasmin). *Retinol binding protein. other:. *alpha-1 (Orosomucoid). *alpha-2 (alpha-2-Macroglobulin, Haptoglobin ... There are alpha and beta lactalbumins; both are contained in milk.. Scientific studies suggest that certain types of ... alpha-1 (Alpha 1-antichymotrypsin, Alpha 1-antitrypsin). *alpha-2 (Alpha 2-antiplasmin) ...
alpha-2 (Ceruloplasmin). *Retinol binding protein. other:. *alpha-1 (Orosomucoid). *alpha-2 (alpha-2-Macroglobulin, Haptoglobin ... Alpha globulins. .mw-parser-output .nobold{font-weight:normal}. serpins:. *alpha-1 (Alpha 1-antichymotrypsin, Alpha 1- ... Alpha-fetoprotein (alpha-fetoglobulin) is a fetal plasma protein that binds various cations, fatty acids and bilirubin. Vitamin ... A number of blood transport proteins are evolutionarily related, including serum albumin, alpha-fetoprotein, vitamin D-binding ...
Alpha-2-Macroglobulin. *Alpha-synuclein. *Amylin. *Aquaporin 1. *Arachidonate 5-lipoxygenase. *Atrophin 1 ...
Alpha-2 zone[edit]. This zone consists principally of alpha-2 macroglobulin (AMG or A2M) and haptoglobin. There are typically ... Alpha-2 macroglobulin may be elevated in children and the elderly. This is seen as a sharp front to the alpha-2 band. AMG is ... Alpha-1 - alpha-2 interzone[edit]. Two faint bands may be seen representing alpha 1-antichymotrypsin and vitamin D binding ... Stevenson, FT; Greene, S; Kaysen, GA (January 1998). "Serum alpha 2-macroglobulin and alpha 1-inhibitor 3 concentrations are ...
巨球蛋白(英語:Macroglobulin)/微球蛋白(英語:Microglobulin) ... Feldmann M, Maini R. Anti-TNF alpha therapy of rheumatoid arthritis: what have we learned?. Annu Rev Immunol. 2001, 19 (1): 163 ... Streptococcal IgA1 protease, digestion, Fab and Fc fragments, and the complete amino acid sequence of the alpha 1 heavy chain. ... α1胰凝乳蛋白酶抑制劑(英語:Alpha
Alpha 1-antichymotrypsin. *Alpha 1-antitrypsin. *Alpha 2-macroglobulin. *C-reactive protein ... Alpha globulins. .mw-parser-output .nobold{font-weight:normal}. serpins:. *alpha-1 (Alpha 1-antichymotrypsin, Alpha 1- ... Orosomucoid (ORM) or alpha-1-acid glycoprotein (α1AGp,[1] AGP or AAG) is an acute phase (acute phase protein) plasma alpha- ... Alpha-1-acid glycoprotein has been identified as one of four potentially useful circulating biomarkers for estimating the five- ...
Endothelial cells do not express TF except when they are exposed to inflammatory molecules such as tumor necrosis factor-alpha ... TNF-alpha). Another cell type that expresses TF on the cell surface in inflammatory conditions is the monocyte (a white blood ...
α granules (alpha granules) - containing P-selectin, platelet factor 4, transforming growth factor-β1, platelet-derived growth ... Platelets store vWF in their alpha granules. When the endothelial layer is disrupted, collagen and VWF anchor platelets to the ... Alpha granules contain clotting mediators such as factor V, factor VIII, fibrinogen, fibronectin, platelet-derived growth ... Platelets contain dense granules, lambda granules and alpha granules. Activated platelets secrete the contents of these ...
Alpha 1-antichymotrypsin. *Alpha 1-antitrypsin. *Alpha 2-macroglobulin. *C-reactive protein ... The chemical structures of the ligands are Ca2+ ion, alpha-D-mannose (C6H12O6), and D-glucosamine (C6H13NO5). ... The structure is mainly made up of single alpha helices shown in red and beta sheets shown in yellow. The two blue structures ...
Placental alpha macroglobulin-1 (PAMG-1) has been the subject of over 20 clinical investigations, the majority of which have ... Placental alpha microglobulin-1 (PAMG-1) is a human protein that was first isolated in 1975 from amniotic fluid. PAMG-1 is an ... Lee, S. E.; Park, J. S.; Norwitz, E. R.; Kim, K. W.; Park, H. S.; Jun, J. K. (2007). "Measurement of placental alpha- ... PAMG-1 was originally referred to as specific alpha-1 globulin of placenta. PAMG-1 is present in blood and the amniotic fluid ...
5 alpha-dihydrotestosterone and [3H]cortisol binding to plasma proteins". J. Steroid Biochem. 33 (2): 251-5. doi:10.1016/0022- ... and as a ligand and inhibitor of α2-macroglobulin. The pharmacokinetics of bicalutamide are unaffected by food, age, body ... role of 3 alpha- and 3 beta-androstanediols". Biology of Reproduction. 51 (3): 562-71. doi:10.1095/biolreprod51.3.562. PMID ...
... amyloid A Alpha 1-lipoprotein Haptoglobin Alpha-2u globulin α2-macroglobulin Ceruloplasmin Thyroxine-binding globulin Alpha 2- ... The alpha globulins typically have molecular weights of around 93 kDa. α1-antitrypsin Alpha 1-antichymotrypsin Orosomucoid ( ... antiplasmin Protein C Alpha 2-lipoprotein Angiotensinogen Cortisol binding globulin Vitamin D-binding protein Alpha-Globulins ... Alpha globulins are a group of globular proteins in plasma that are highly mobile in alkaline or electrically charged solutions ...
Alpha-2 macroglobulin may be elevated in children and the elderly. This is seen as a sharp front to the alpha-2 band. AMG is ... Stevenson, FT; Greene, S; Kaysen, GA (January 1998). "Serum alpha 2-macroglobulin and alpha 1-inhibitor 3 concentrations are ... The alpha-1 fraction does not disappear in alpha 1-antitrypsin deficiency, however, because other proteins, including alpha- ... resulting in a typical elevation in the alpha-2 zone during inflammation. A normal alpha-2 and an elevated alpha-1 zone is a ...
... for proteinuria evaluates the levels of various serum proteins in the urine, e.g. Albumin, Alpha-2-macroglobulin and ...
Buy our Natural Human alpha 2 Macroglobulin protein. Ab91104 is a full length protein produced in Nativesyntheticaly and has ... Lane 4. 5 µg alpha 2 Macroglobulin (reduced/ heated) Lane 5. 10 µg alpha 2 Macroglobulin (reduced/ heated) Lane 6. 20 µg alpha ... 5 µg alpha 2 Macroglobulin (non-reduced/no heat) Lane 2. 10 µg alpha 2 Macroglobulin (non-reduced/no heat) Lane 3. 20 µg alpha ... Natural Human alpha 2 Macroglobulin protein. See all alpha 2 Macroglobulin proteins and peptides. ...
... alpha-2-macroglobulin) for WB. Anti-alpha 2 Macroglobulin pAb (GTX112856) is tested in Human samples. 100% Ab-Assurance. ... alpha-2-macroglobulin antibody, alpha-2-M antibody, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 antibody ... alpha-2-macroglobulin. Background. Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many ... Storage Conditions: alpha 2 Macroglobulin antibody [N2N3]. Storage Buffer. 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal ...
... Download the ... 12, 2017 /PRNewswire/ -- Complement C5 (C3 And PZP Like Alpha 2 Macroglobulin Domain Containing Protein 4 or C5) - Pipeline ... Complement C5 (C3 And PZP Like Alpha 2 Macroglobulin Domain Containing Protein 4 or C5) - Pipeline Review, H2 2017. Friday, ... It also reviews key players involved in Complement C5 (C3 And PZP Like Alpha 2 Macroglobulin Domain Containing Protein 4 or C5 ...
... macroglobulin complexes isolated from the serum of a mammal. The invention also relates to methods for making such complexes ... The present invention relates to the use of alpha (2) ... The Alpha (2) Macroglobulin Receptor, or "CD91" The alpha (2) ... macroglobulin-binding molecule is an antibody specific to alpha (2) macroglobulin. The alpha (2) macroglobulin-specific ... Alpha (2) Macroglobulin The .alpha.-macroglobulins are members of a protein superfamily of structurally related proteins which ...
C3 And PZP Like Alpha 2 Macroglobulin Domain Containing Protein 4 or C5) pipeline Target constitutes close to 33 molec ... C3 And PZP Like Alpha 2 Macroglobulin Domain Containing Protein 4 or C5) - Overview. Complement C5 (C3 And PZP Like Alpha 2 ... Complement C5 (C3 And PZP Like Alpha 2 Macroglobulin Domain Containing Protein 4 or C5) - Drug Profiles. AcPepA - Drug Profile ... Complement C5 (C3 And PZP Like Alpha 2 Macroglobulin Domain Containing Protein 4 or C5) - Dormant Products. Complement C5 (C3 ...
Precipitation by electrophoresis is in the alpha region. They include alpha 1-macroglobulins and alpha 2-macroglobulins. These ... "alpha-Macroglobulins" by people in this website by year, and whether "alpha-Macroglobulins" was a major or minor topic of these ... "alpha-Macroglobulins" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical ... Reduced trypsin-binding capacity of alpha 2-macroglobulin in the peritoneal fluid of women with endometriosis: possible ...
alpha 2-Macroglobulin Synonym A2M; alpha 2Macroglobulin; alpha 2-Macroglobulin; alpha-2-M; alpha-2-macroglobulin; C3 and PZP- ... The loss of alpha-2-macroglobulin into urine is prevented by its large size. The net result is that alpha-2-macroglobulin ... and no disease state is attributed to low concentrations of alpha-2-macroglobulin. The concentration of alpha-2-macroglobulin ... Alpha-2-Macroglobulin (α2M) is a large (720 KDa) plasma protein found in the blood. It is mainly produced by the liver, and ...
The protease inhibitor alpha-2-macroglobulin-like-1 is the p170 antigen recognized by paraneoplastic pemphigus autoantibodies ... This gene encodes a member of the alpha-macroglobulin superfamily. The encoded protein is thought to be an N-glycosylated ... Studies support a role for alpha-2-macroglobulin-like 1 protein (A2ML1) in the pathophysiology of otitis media.. ... Anti-?-2-macroglobulin-like-1 autoantibodies are detected frequently and may be pathogenic in paraneoplastic pemphigus.. ...
Rat Alpha_2 Macroglobulin ELISA \ KT-353 for more molecular products just contact us ... We have also other products like : Rat Alpha_2 Macroglobulin ELISA. Related products : Rat Alpha_2 Macroglobulin ELISA ... Akt1] RAC-alpha serine/threonine-protein kinase (EC 2.7.11.1) (Protein kinase B) (PKB) (Protein kinase B alpha) (PKB alpha) ( ... Major urinary protein (MUP) (Allergen Rat n I) (Alpha(2)-euglobulin) (Alpha-2u-globulin) (alpha-2u globulin PGCL1) (allergen ...
... implications for the binding mechanism of alpha 2-macroglobulin. ... alpha 2-Macroglobulin (alpha 2M) is a complex tetrameric ... Nonproteolytic incorporation of protein ligands into human alpha 2-macroglobulin: ... Nonproteolytic incorporation of protein ligands into human alpha 2-macroglobulin: implications for the binding mechanism of ... The thiol ester in alpha 2M was cleaved by NH3. After removal of excess NH3, the alpha 2M derivative was incubated with excess ...
This is a domain of the alpha-2-macroglobulin family.. The alpha-macroglobulin (aM) family of proteins includes protease ... This family includes a region of the alpha-2-macroglobulin family.. Interpro abstract (IPR011625):. ... inhibitors [(PUBMED:2473064)], typified by the human tetrameric a2-macroglobulin (a2M); they belong to the MEROPS proteinase ...
Rat alpha 1-macroglobulin (alpha 1M), rat alpha 2-macroglobulin (alpha 2M) migrated as single bands on non-denaturing gels when ... rat alpha 1M, rat alpha 2M and mouse alpha 2M, respectively. Only rat alpha 2M, the acute-phase alpha-macroglobulin studied, ... The other alpha-macroglobulin-methylamine complexes cleared faster than the inhibitors that had not reacted. Rat alpha 2M and ... A single saturable pathway rapidly cleared complexes of trypsin and the alpha-macroglobulins of mouse, rat and human from the ...
The binding of 125I-labelled transforming growth factor-beta 1 (TGF-beta 1) to human alpha 2-macroglobulin (alpha 2M) was ... TGF-beta 1 bound preferentially to alpha 2M-methylamine and minimally, if at all, to native alpha 2M. Preparations of alpha 2M- ... alpha 2M-trypsin and alpha 2M-chymotrypsin prepared at an r value of 1.0 counteracted the activity of TGF-beta 1, whereas the ... Binding of transforming growth factor-β1 to methylamine-modified α2-macroglobulin and to binary and ternary α2-macroglobulin- ...
The loss of alpha-2-macroglobulin into urine is prevented by its large size. The net result is that alpha-2-macroglobulin ... Overall, alpha-2-Macroglobulin is the largest major nonimmunoglobulin protein in human plasma. The alpha-macroglobulin (aM) ... 10-15% of copper in human plasma is chelated by alpha-2-macroglobulin. alpha-2-Macroglobulin levels are increased when the ... of alpha-2-macroglobulin leads to increased risk of Alzheimers disease. alpha-2-Macroglobulin binds to and removes the active ...
... ,ARUP Laboratories is a national reference laboratory and a worldwide leader in innovative laboratory ...
Ma H, Li RZ, Zhang ZY, Tong TJ (2004) mRNA level of alpha-2-macroglobulin as an aging biomarker of human fibroblasts in culture ... Alpha-2-macroglobulin (α2Μ) Electrophoretic mobility shift assays (EMSA) Functional promoter region determination α2M ... Ma L.W., Li G.D., Tong T.J. (2014) Upregulation of Alpha-2-Macroglobulin in Replicative Senescence. In: Hayat M. (eds) Tumor ... Lin VK, Wang SY, Boetticher NC, Vazquez DV, Saboorian H, McConnell JD, Roehrborn CG (2005) Alpha(2) macroglobulin, a PSA ...
A conserved region in alpha-macroglobulins participates in binding to the mammalian alpha-macroglobulin receptor.. Biochemistry ... Proteinase binding and inhibition by the monomeric alpha-macroglobulin rat alpha 1-inhibitor-3.. J. Biol. Chem. 264 11428-35 ... The alpha-macroglobulin (aM) family of proteins includes protease inhibitors [PMID: 2473064], typified by the human tetrameric ... Alpha-macroglobulins: structure, shape, and mechanism of proteinase complex formation.. J. Biol. Chem. 264 11539-42 1989 ...
alpha 2M from Limulus shows many of the typical characteristics of mammalian alpha 2M, including the presence of an internal ... alpha 2M) in the hemolymph plasma and hemocytes. ... The American horseshoe crab Limulus polyphemus contains alpha 2 ... Trypsin-treated Limulus alpha 2M, but not the native alpha 2M, displaced methylamine-treated human 125I-alpha 2M from the human ... The American horseshoe crab Limulus polyphemus contains alpha 2-macroglobulin (alpha 2M) in the hemolymph plasma and hemocytes ...
alpha 2 macroglobulin · biological marker · gelatinase A · gelatinase B · hydroxyproline · interstitial collagenase · matrix ... Biomedical Research · alpha 2 macroglobulin · interstitial collagenase · matrix metalloproteinase · prointerstitial collagenase ... Chemicals / CAS: alpha 2 macroglobulin, 95568-41-5; gelatinase A, 146480-35-5; gelatinase B, 146480-36-6; hydroxyproline, 51-35 ... A2-antiplasmin · Activity assay · Automated · Plasmin inhibitor · Adult · alpha-Macroglobulins · Antifibrinolytic Agents · ...
Abcam provides specific protocols for Anti-alpha 2 Macroglobulin antibody (HRP) (ab7339) : Immunohistochemistry protocols, ...
Browse our alpha 2-Macroglobulin Lysate catalog backed by our Guarantee+. ... alpha 2-Macroglobulin lysate, A2M lysate, ALPL lysate, alpha-2-M lysate, alpha-2-macroglobulin lysate, C3 and PZP-like alpha-2- ... alpha 2-Macroglobulin Lysates. We offer alpha 2-Macroglobulin Lysates for use in common research applications: Western Blot. ... Our alpha 2-Macroglobulin Lysates can be used in a variety of model species: Human. Use the list below to choose the alpha 2- ...
Browse our alpha 2-Macroglobulin Protein catalog backed by our Guarantee+. ... alpha 2-Macroglobulin Proteins. We offer alpha 2-Macroglobulin Peptides and alpha 2-Macroglobulin Proteins for use in common ... alpha 2-Macroglobulin protein, A2M protein, ALPL protein, alpha-2-M protein, alpha-2-macroglobulin protein, C3 and PZP-like ... Our alpha 2-Macroglobulin Peptides and alpha 2-Macroglobulin Proteins can be used in a variety of model species: Human. Use the ...
Characterization of alpha 2-macroglobulin binding to human trabecular meshwork cells: Presence of the alpha 2-macroglobulin ... 1992) Alpha 2-macroglobulin, a multifunctional binding protein with targeting characteristics. FASEB J 6(15):3345-3353. ... 2006) Lack of site-specific production of decidual alpha-2 macroglobulin in human pregnancy. J Soc Gynecol Investig 13(7):491- ... 1994) Expression of the alpha 2-macroglobulin-encoding gene in rat brain and cultured astrocytes. Gene 141(2):155-162. ...
What is Alpha 2-macroglobulin? Meaning of Alpha 2-macroglobulin medical term. What does Alpha 2-macroglobulin mean? ... Looking for online definition of Alpha 2-macroglobulin in the Medical Dictionary? Alpha 2-macroglobulin explanation free. ... alpha-2 macroglobulin. (redirected from Alpha 2-macroglobulin). Also found in: Wikipedia. alpha-2 macroglobulin. Plasma ... Structure of the human alpha 2-macroglobulin gene and its promoter.. Alpha 2 macroglobulin activity in rats infected with ...
Although alpha 2-macroglobulin has been identified as the inhibitor in horse serum, the inhibitors in pig and rabbit sera have ... The inhibitor corresponded in size to alpha 2-macroglobulin and cross-reacted antigenically with human alpha 2-macroglobulin. ... Alpha 2-macroglobulin is the major neutralizing inhibitor of influenza A virus in pig serum Virology. 1993 Apr;193(2):974-6. ... 6-galactose but not N-acetylneuraminic acid-alpha 2,3-galactose. These data indicate that alpha 2-macroglobulin is the major ...
To investigate the binding of the prostate-specific antigen (PSA) to human alpha 2-macroglobulin (alpha 2-M) and to alpha 1- ... Prostate-specific antigen forms complexes with human alpha 2-macroglobulin and binds to the alpha 2-macroglobulin receptor/LDL ... Complexes of PSA-alpha 2-M and PSA-ACT were found to bind to the alpha 2-macroglobulin receptor/LDL receptor-related protein ( ... Kinetic analysis revealed faster binding of PSA to alpha 2-M than to ACT. The PSA bound to alpha 2-M is caged by the inhibitor ...
Analysis of Alpha-2 Macroglobulin from the Long-Lived and Cancer-Resistant Naked Mole-Rat and Human Plasma.. Thieme R1, Kurz S1 ... Analysis of Alpha-2 Macroglobulin from the Long-Lived and Cancer-Resistant Naked Mole-Rat and Human Plasma ... Analysis of Alpha-2 Macroglobulin from the Long-Lived and Cancer-Resistant Naked Mole-Rat and Human Plasma ... Analysis of Alpha-2 Macroglobulin from the Long-Lived and Cancer-Resistant Naked Mole-Rat and Human Plasma ...
Crystal structure of native alpha-2-macroglobulin from Escherichia coli spanning the residues from domain MG7 to the C-terminus ... Crystal structure of native alpha-2-macroglobulin from Escherichia coli spanning the residues from domain MG7 to the C-terminus ... Structural and functional insights into Escherichia coli alpha 2-macroglobulin endopeptidase snap-trap inhibition.. Garcia- ... Gram-negative bacteria from the human gut microbiome encode a relative of the human endopeptidase inhibitor, α2-macroglobulin ( ...
A Role for Alpha-2-Macroglobulin in the Cancer Immunity of Naked Mole-Rats. Permalink No Comments Yet Add a Comment Posted by ... Here researchers outline a role for alpha-2-macroglobulin (A2M) in cancer suppression; it appears to inhibit tumor growth in ...
Buy goat anti-human Alpha 2-Macroglobulin polyclonal antibody for research, development and IVD manufacturing uses - Inquire ... Polyclonal anti-human alpha 2 macroglobulin (a2M) procedure goats were immunized with highly purified human alpha 2- ... macroglobulin isolated from human serum. The resulting anti-human alpha-2 Macroglobulin (a2M) was solid phase absorbed then ... This anti-human alpha 2-macroglobulin (A2M) is suitable for depletion, ELISA and Blotting applications. ...
Alpha-2 macroglobulin was purified and alpha-1 antiprotease was partially purified from human sera by other methods and shown ... A high molecular weight inhibitory fraction was identified as predominantly alpha-2 macroglobulin and a low molecular weight ... Preparative isoelectric focusing of human serum isolated inhibitory fractions containing these same alpha globulins. ... inhibitory fraction was identified as predominantly alpha-1 antiprotease. ...
Alpha-2-macroglobulin (α2-M), a serum protease inhibitor that also binds cytokines, neutralized the inhibitory effect exerted ... Effect of alpha-2-macroglobulin on cytokine-mediated human C-reactive protein production. *Andrew W. Taylor1. & ... Alpha-2-macroglobulin (α2-M), a serum protease inhibitor that also binds cytokines, neutralized the inhibitory effect exerted ... Taylor, A.W., Mortensen, R.F. Effect of alpha-2-macroglobulin on cytokine-mediated human C-reactive protein production. ...
"α2-Macroglobulin (α2M) is an extracellular chaperone that inhibits amorphous and fibrillar protein aggregation. The reaction of ... Protease-activated alpha-2-macroglobulin can inhibit amyloid formation via two distinct mechanisms ... Protease-activated alpha-2-macroglobulin can inhibit amyloid formation via two distinct mechanisms. FEBS Letters, 587 (5), 398- ... α2-Macroglobulin (α2M) is an extracellular chaperone that inhibits amorphous and fibrillar protein aggregation. The reaction of ...
Alpha-2-macroglobulin Antibody 13545-1-AP has been identified with IF, IHC, IP, WB, ELISA. 13545-1-AP detected 185 kDa band in ... alpha-2-macroglobulin, also known as α2-macroglobulin (α2M or A2M), is a protein abundant in the plasma of vertebrates and ... A2M, Alpha 2 M, alpha 2 macroglobulin, CPAMD5, FWP007, S863 7, α-2M ... Alpha-2-macroglobulin Antibody 0 Publications. Rabbit Polyclonal, Catalog number: 13545-1-AP ...
Interactions of pregnancy associated plasma protein a and alpha 2 macro globulin on metal chelate affinity chromatography ... Evolution of alpha 2 macro globulin the structure of a protein homologous with human alpha 2 macro globulin from plaice ... Studies on alpha 2 macro globulin in bovine plasma part 2 interaction of alpha 2 macro globulin and trypsin. Journal of ... Evolution of alpha 2 macro globulin the purification and characterization of a protein homologous with human alpha 2 macro ...
... that binds human alpha-2-macroglobulin (also known as A2M). Validated for ELISA and western blot. ... Alpha-2-macroglobulin antibody LS-C189385 is a purified mouse monoclonal (clone SPM178, isotype IgG1, kappa) ... Anti-A2M / Alpha-2-Macroglobulin Antibody (HRP) LS-C25313 Antibody:. Goat Polyclonal (IgG) to Human A2M / Alpha-2-Macroglobulin ... Anti-A2M / Alpha-2-Macroglobulin Antibody (C-Terminus) LS-C46958 Antibody:. Rabbit Polyclonal to Human A2M / Alpha-2- ...
Alpha-2 macroglobulin (A2M) is a large plasma protein found in the blood. A2M is produced by the liver, and is a major ... component of the alpha-2 band in protein elec ... Rat Alpha-2 Macroglobulin, Alpha-2 macroglobulin (A2M) is a ... studies showed that the comparison of the amino acid compositions of Alpha-2 macroglobulin from Rat and Alpha-2 macroglobulin ... It was reported that the conformation of rat acute-phase Alpha-2 macroglobulin , both at the molecular and secondary structural ...
Alpha 2 Macroglobulin Antigen-NP_000005.2 (MBS568833) product datasheet at MyBioSource, Antigens. Application: Immunogen Grade ... Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin ... Belongs to the protease inhibitor I39 (alpha-2- macroglobulin) family.. Protein type: Inhibitor; Secreted; Secreted, signal ... 1. we identify a single serum factor, alpha2-macroglobulin (alpha2M), which is both required and sufficient for rosetting ...
Alpha 2 Macroglobulin Antigen-NP_000005.2 (MBS568833) product datasheet at MyBioSource, Antigens. Application: Immunogen Grade ... Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin ... Belongs to the protease inhibitor I39 (alpha-2- macroglobulin) family.. Protein type: Inhibitor; Secreted; Secreted, signal ... Alpha-centractin (ACTR1A) Recombinant Protein. • heparan sulfate (glucosamine) 3-O-sulfotransferase 1 (HS3ST1... ELISA Kit. • ...
  • The concentration of alpha-2-macroglobulin rises 10-fold or more in the nephrotic syndrome when other lower molecular weight proteins are lost in the urine. (assaysolution.com)
  • ELISA: Human alpha 2-Macroglobulin ELISA Kit (Colorimetric) - These standard curves are provided for demonstration only. (assaysolution.com)
  • alpha 2-Macroglobulin (alpha 2M) is a complex tetrameric protein of 718 kDa. (duke.edu)
  • This assay has high sensitivity and excellent specificity for detection of human alpha 2-Macroglobulin. (assaysolution.com)
  • Reduced trypsin-binding capacity of alpha 2-macroglobulin in the peritoneal fluid of women with endometriosis: possible relevance to alterations in macrophase function. (uchicago.edu)
  • No significant cross-reactivity or interference between human alpha 2-Macroglobulin and analogues was observed. (assaysolution.com)
  • Nonproteolytic incorporation of protein ligands into human alpha 2-macroglobulin: implications for the binding mechanism of alpha 2-macroglobulin. (duke.edu)
  • The net result is that alpha-2-macroglobulin reaches serum levels equal to or greater than those of albumin in the nephrotic syndrome, which has the effect of maintaining oncotic pressure. (assaysolution.com)
  • Cleavage of the thiol ester with small nucleophiles destabilizes the native conformation and causes a major conformational change in alpha 2M, which leads to exposure of receptor binding sites and a change in electrophoretic mobility. (duke.edu)
  • Receptor binding studies and native pore-limit PAGE confirmed that the alpha 2M derivatives with ligand incorporated remained in the receptor-recognized, 'fast' migrating conformation. (duke.edu)
  • This is the first demonstration of nonproteolytic, covalent incorporation of protein ligands into receptor-recognized alpha 2M. (duke.edu)
  • This gene encodes a member of the alpha-macroglobulin superfamily. (nih.gov)
  • Recently it has been shown that nucleophilic cleavage of the four thiol esters in alpha 2M is a reversible process with energy requirements dependent on the nucleophile [Grøn, H., Thøgersen, I. B., Enghild, J. J., and Pizzo, S. V. (1996) Biochem. (duke.edu)
  • Precipitation by electrophoresis is in the alpha region. (uchicago.edu)
  • The present study is a further investigation of the properties of alpha 2M with cleaved thiol esters and the potential for incorporation of protein ligands at the site of the thiol ester. (duke.edu)
  • This graph shows the total number of publications written about "alpha-Macroglobulins" by people in this website by year, and whether "alpha-Macroglobulins" was a major or minor topic of these publications. (uchicago.edu)
  • No specific deficiency with associated disease has been recognized, and no disease state is attributed to low concentrations of alpha-2-macroglobulin. (assaysolution.com)
  • Alpha-2-Macroglobulin (α2M) is a large (720 KDa) plasma protein found in the blood. (assaysolution.com)
  • Studies support a role for alpha-2-macroglobulin-like 1 protein (A2ML1) in the pathophysiology of otitis media. (nih.gov)
  • In native alpha 2M, each of the four subunits contains a thiol ester between the side chains of Cys949 and Gln952. (duke.edu)
  • After removal of excess NH3, the alpha 2M derivative was incubated with excess protein ligand (hen egg lysozyme or bovine insulin) at 23, 37, or 50 degreesC, leading to covalent incorporation of the ligands in alpha 2M as analyzed by SDS-PAGE, gel filtration, and centrifugal microfiltration. (duke.edu)
  • alpha-2-Macroglobulin (α2M) is a large (720 KDa) plasma protein found in the blood. (wikipedia.org)
  • Alpha-2-macroglobulin may act as a carrier protein because it also binds to numerous growth factors and cytokines, such as platelet-derived growth factor, basic fibroblast growth factor, TGF-β, insulin, and IL-1β. (wikipedia.org)
  • Overall, alpha-2-Macroglobulin is the largest major nonimmunoglobulin protein in human plasma. (wikipedia.org)
  • a sequence of amino acids in an α2-macroglobulin molecule, or a homologous protein, that contains scissile peptide bonds for those proteinases that it inhibits) and a thiol ester, (iii) a similar protease inhibitory mechanism and (iv) the inactivation of the inhibitory capacity by reaction of the thiol ester with small primary amines. (wikipedia.org)
  • Human pregnancy zone protein and alpha 2-macroglobulin. (ebi.ac.uk)
  • We offer alpha 2-Macroglobulin Peptides and alpha 2-Macroglobulin Proteins for use in common research applications: Blocking/Neutralizing, Control, ELISA, Protein Array, SDS-Page, Western Blot. (novusbio.com)
  • Each alpha 2-Macroglobulin Peptide and alpha 2-Macroglobulin Protein is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. (novusbio.com)
  • We demonstrate that BK Ca interacts with the immunomodulator α-2-macroglobulin (α 2 M) and its receptor low-density lipoprotein receptor-related protein 1 in human uterine muscle cells isolated from pregnant women. (pnas.org)
  • From this screen, we identified alpha-2-macroglobulin (α 2 M). We then used immunoprecipitation followed by immunoblot and the proximity ligation assay to confirm the interaction between BK Ca and both α 2 M and its receptor, low-density lipoprotein receptor-related protein 1 (LRP1), in cultured primary human myometrial smooth muscle cells (hMSMCs). (pnas.org)
  • Alpha 2-macroglobulin , a multifunctional binding protein with targeting characteristics. (thefreedictionary.com)
  • Complexes of PSA-alpha 2-M and PSA-ACT were found to bind to the alpha 2-macroglobulin receptor/LDL receptor-related protein (alpha 2-M-R/LRP) which may be the clearance receptor for PSA. (nih.gov)
  • Recombinant protein encompassing a sequence within the center region of human alpha 2 Macroglobulin. (genetex.com)
  • Download the full report: https://www.reportbuyer.com/product/4368352 Summary Complement C5 (C3 And PZP Like Alpha 2 Macroglobulin Domain Containing Protein 4 or C5) - Complement component 5 is a protein is encoded by the C5 gene. (medindia.net)
  • C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.Complement C5 (C3 And PZP Like Alpha 2 Macroglobulin Domain Containing Protein 4 or C5) pipeline Target constitutes close to 31 molecules. (medindia.net)
  • It also reviews key players involved in Complement C5 (C3 And PZP Like Alpha 2 Macroglobulin Domain Containing Protein 4 or C5) targeted therapeutics development with respective active and dormant or discontinued projects.The report is built using data and information sourced from proprietary databases, company/university websites, clinical trial registries, conferences, SEC filings, investor presentations and featured press releases from company/university sites and industry-specific third party sources. (medindia.net)
  • Alpha-2-macroglobulin ( α 2-M), a serum protease inhibitor that also binds cytokines, neutralized the inhibitory effect exerted by transforming growth factor- Β (TGF- Β ) on IL-6-induced C-reactive protein (CRP) production by the human hepatoma cell line PLC/PRF/5. (springer.com)
  • α2-Macroglobulin (α2M) is an extracellular chaperone that inhibits amorphous and fibrillar protein aggregation. (edu.au)
  • alpha-2-macroglobulin, also known as α2-macroglobulin (α2M or A2M), is a protein abundant in the plasma of vertebrates and several invertebrates. (ptglab.com)
  • A2M is produced by the liver, and is a major component of the alpha-2 band in protein electrophoresis. (innov-research.com)
  • alpha-2 Macroglobulin receptor/Ldl receptor-related protein(Lrp)-dependent internalization of the urokinase receptor. (rupress.org)
  • Both LB6 clone 19 cells, a mouse cell line transfected with the human uPAR cDNA, and the human U937 monocytic cell line, express in addition to uPAR also the endocytic alpha 2-macroglobulin receptor/low density lipoprotein receptor-related protein (LRP/alpha 2-MR) which is required to internalize uPAR-bound uPA-PAI-1 and uPA-PN-1 complexes. (rupress.org)
  • Studies support a role for alpha-2-macroglobulin-like 1 protein (A2ML1) in the pathophysiology of otitis media. (nih.gov)
  • Alpha 2 macroglobulin is a protein. (livelongstayyoung.com)
  • Discovery of FAC as a biomarker for osteoarthritis led to the identification of a naturally-occurring blood protein, Alpha-2 Macroglobulin (A2M), which protects cartilage from degradation and can alleviate joint pain. (cytonics.com)
  • The 1474-amino acid protein is a member of the Protease inhibitor I39 (alpha-2-macroglobulin) family. (biocompare.com)
  • Alpha 2 macroglobulin (A2M) uses the most advanced science to not only stop osteoarthritis at its source, but also protect you from a protein complex that causes your joints to ache. (thrivemdvail.com)
  • A2ML1 (Alpha-2-Macroglobulin Like 1) is a Protein Coding gene. (genecards.org)
  • Alpha 2 Macroglobulin (A2M) is a naturally occurring protein that is found in joints and in a person's blood. (checkbiotech.org)
  • These included proteins already studied in the context of CVD (such as apolipoprotein B, alpha-2-macroglobulin), as well as novel findings (such as low density lipoprotein receptor related protein 2 [LRP2], protein SZT2) for which a mechanism of action is suggested. (springer.com)
  • CPAMD8 (C3 And PZP Like Alpha-2-Macroglobulin Domain Containing 8) is a Protein Coding gene. (genecards.org)
  • Nonproteolytic incorporation of protein ligands into human alpha 2-macroglobulin: implications for the binding mechanism of alpha 2-macroglobulin. (duke.edu)
  • alpha 2-Macroglobulin (alpha 2M) is a complex tetrameric protein of 718 kDa. (duke.edu)
  • The present study is a further investigation of the properties of alpha 2M with cleaved thiol esters and the potential for incorporation of protein ligands at the site of the thiol ester. (duke.edu)
  • After removal of excess NH3, the alpha 2M derivative was incubated with excess protein ligand (hen egg lysozyme or bovine insulin) at 23, 37, or 50 degreesC, leading to covalent incorporation of the ligands in alpha 2M as analyzed by SDS-PAGE, gel filtration, and centrifugal microfiltration. (duke.edu)
  • This is the first demonstration of nonproteolytic, covalent incorporation of protein ligands into receptor-recognized alpha 2M. (duke.edu)
  • In the present study, this protein was characterised as alpha2 macroglobulin (alpha2M) and the kinetics of its production and its influence on the malignancy of the disease were determined. (bvsalud.org)
  • The α-macroglobulins are members of a protein superfamily of structurally related proteins which also comprises complement components C3, C4 and C5. (justia.com)
  • The human plasma protein alpha (2) macroglobulin (α2M) is a 720 kDa homotetrameric protein primarily known as a proteinase inhibitor and plasma and inflammatory fluid proteinase scavenger molecule (for review see Chu and Pizzo, 1994, Lab. (justia.com)
  • The other proteins were significantly lower at birth than adult values but after 6 months, only albumin, prealbumin, retinol-binding protein, and alpha 1-acid glycoprotein still remained lower in infants. (biomedsearch.com)
  • Pregnancy zone protein, a proteinase-binding macroglobulin. (wikipedia.org)
  • The α 2 -macroglobulin receptor (α 2 MR) or low-density lipoprotein receptor-related protein (LRP) is a cell-surface receptor synthesized as a 600-kd precursor protein (1, 2). (springer.com)
  • Sequence identity between the a2-macroglobulin receptor and low density lipoprotein receptor-related protein suggests that this molecule is a multifunctional receptor. (springer.com)
  • Kristensen T, Moestrup SK, Gliemann J, Bendtsen L, Sand O, Sottrup-Jensen L. Evidence that the newly cloned low-density-lipoprotein receptor related (LRP) protein is the α2-macroglobulin receptor. (springer.com)
  • Purified α 2 -macroglobulin receptor/ LDL receptor-related protein binds urokinase: plasminogen activator inhibitor type-1 complex. (springer.com)
  • Clearance of chylomicron remnants by the low density lipoprotein receptor-related protein/α 2 -macroglobulin receptor. (springer.com)
  • Cytonics' recombinant drug development program is anchored in robust preclinical data indicating that the proteinase inhibitor alpha-2-macroglobulin critically inhibits cartilage breakdown in models of osteoarthritis. (cytonics.com)
  • alpha-2-Macroglobulin binds to and removes the active forms of the gelatinase (MMP-2 and MMP-9) from the circulation via scavenger receptors on the phagocytes. (wikipedia.org)
  • Prostate-specific antigen forms complexes with human alpha 2-macroglobulin and binds to the alpha 2-macroglobulin receptor/LDL receptor-related pro. (nih.gov)
  • Alpha (2) macroglobulin promiscuously binds to proteins and peptides with nucleophilic amino acid side chains in a covalent manner (Chu et al. (justia.com)
  • Alpha2-macroglobulin: an evolutionarily conserved arm of the innate immune system. (ebi.ac.uk)
  • Transcriptome analysis of NMR liver compared to wild-derived mice revealed very high expression of cell adhesion molecules involved in tumour development as well as the pan- proteinase inhibitor alpha2-macroglobulin (A2M). (fightaging.org)
  • I have been using Cytonics' alpha2- macroglobulin kits to treat various joint pains mostly in the knee. (cytonics.com)
  • Proteolytic and hemorrhagic activities of BaP1 were readily inhibited by the plasma proteinase inhibitor, alpha2-macroglobulin, and normal mouse serum also inhibited hemorrhage. (lstmed.ac.uk)
  • The aim of this study was to observe the pattern of alteration of serum alpha 2 macroglobulin (AMG), alpha 1- antitrypsin (AT) and alpha 1-antichymotrypsin (ACT) activities in renal transplant patients receiving the immunosuppressive drug, cyclosporine. (elsevier.com)
  • Serum antiproteinase activities of serum alpha 2 macroglobulin (AMG), alpha 1- antitrypsin (AT) and alpha 1-antichymotrypsin (ACT) were found to be altered in renal transplant patients receiving cyclosporine. (elsevier.com)
  • The entire amino acid sequence is similar to those of the mammalian alpha 2Ms (28-29% identity) and contains common features found in mammalian alpha 2Ms. (nih.gov)
  • However, the N-terminal portion (positions 24-105) has no sequence similarity with those of mammalian alpha 2Ms, and it is structurally related to that of the human complement factor C8 chain, consistent with a role for Limulus alpha 2M in host defense. (nih.gov)
  • The component sugar analysis of Limulus alpha 2M showed the existence of a complex type of oligosaccharide chain similar to those of mammalian alpha 2M. (nih.gov)
  • However, unlike mammalian alpha 2M, no sialic acid was detected in Limulus alpha 2M and it contained approximately 3 mol/mol N-acetylgalactosamine, suggesting the presence of O-linked sugar chains, which have not been found in mammalian alpha 2M. (nih.gov)
  • Expresses full-length Alpha-2-macroglobulin precursor ectodomain in mammalian cells. (addgene.org)
  • No significant cross-reactivity or interference between human alpha 2-Macroglobulin and analogues was observed. (assaysolution.com)
  • Alpha 2 macroglobulin captures and inhibits the activity of these four groups of proteases. (livelongstayyoung.com)
  • The LRP1 agonists, α 2 -macroglobulin and tissue-type plasminogen activator, attenuated expression of inflammatory mediators, even in the presence of LPS. (pnas.org)
  • No interference was detected from a2- macroglobulin, lysine, unfractionated and low-molecular weight heparln, and ε-amino caproic acid or from the non-plasminogen binding form of plasmin inhibitor. (tudelft.nl)
  • Molecular cloning and expression analysis of alpha 2-macroglobulin in the kuruma shrimp Marsupenaeus japonicus. (thefreedictionary.com)
  • A high molecular weight inhibitory fraction was identified as predominantly alpha-2 macroglobulin and a low molecular weight inhibitory fraction was identified as predominantly alpha-1 antiprotease. (ajtmh.org)
  • It was reported that the conformation of rat acute-phase Alpha-2 macroglobulin , both at the molecular and secondary structural levels, is strikingly similar to that of human Alpha-2 macroglobulin. (innov-research.com)
  • This short highly conserved region of proteinase-binding alpha-macro-globulins contains the cysteine and a glutamine of a thiol-ester bond that is cleaved at the moment of proteinase binding, and mediates the covalent binding of the alpha-macro-globulin to the proteinase. (ebi.ac.uk)
  • In native alpha 2M, each of the four subunits contains a thiol ester between the side chains of Cys949 and Gln952. (duke.edu)
  • The thiol ester in alpha 2M was cleaved by NH3. (duke.edu)
  • Although alpha 2-macroglobulin has been identified as the inhibitor in horse serum, the inhibitors in pig and rabbit sera have not been identified. (nih.gov)
  • As alpha-2- M and alpha-I- AT are established plasmin inhibitors, our results suggest that in pre-eclampsia there is an exaggeration of the depression in fibrinolytic activity found in normal pregnancy. (who.int)
  • Proteinase binding and inhibition by the monomeric alpha-macroglobulin rat alpha 1-inhibitor-3. (ebi.ac.uk)
  • Free active MMP is unlikely to be present in plasma: upon entering the circulation, active MMP is expected to be captured by the proteinase inhibitor α2-macroglobulin (α2M). (tudelft.nl)
  • The inhibitor corresponded in size to alpha 2-macroglobulin and cross-reacted antigenically with human alpha 2-macroglobulin. (nih.gov)
  • These data indicate that alpha 2-macroglobulin is the major neutralizing inhibitor of influenza A virus in pig serum. (nih.gov)
  • The proteinase inhibitor alpha 2-M has to be considered as a main contributor to PSA complex formation in the blood. (nih.gov)
  • Gram-negative bacteria from the human gut microbiome encode a relative of the human endopeptidase inhibitor, α2-macroglobulin (α2M). (rcsb.org)
  • In short, alpha 2 macroglobulin is a master inhibitor of cartilage degrading enzymes. (livelongstayyoung.com)
  • Alpha 2-Macroglobulin (α2M) is a large proteinase inhibitor molecule of 718,000 daltons, consisting of 4 identical subunits of 185,000 each. (affinitybiologicals.com)
  • A2M (Alpha-2-Macroglobulin) is a newly discovered compound that acts as a powerful inhibitor of cartilage breakdown. (nonamedicalarts.com)
  • We recently reported that levels alpha‐2‐macroglobulin (A2MG)‐containing microparticles are elevated in plasma from patients with sepsis. (embopress.org)
  • Characterization of the inhibitor's oligosaccharide moiety using linkage-specific lectins revealed the presence of N-acetylneuraminic acid-alpha 2,6-galactose but not N-acetylneuraminic acid-alpha 2,3-galactose. (nih.gov)
  • Polyclonal anti-human alpha 2 macroglobulin (a2M) procedure goats were immunized with highly purified human alpha 2-macroglobulin isolated from human serum. (leebio.com)
  • Preparative isoelectric focusing of human serum isolated inhibitory fractions containing these same alpha globulins. (ajtmh.org)
  • Do not freeze taw, rather use Alpha_2_Macroglobulin, Goat anti_Human, Rabbit, Bovine, Swine, Dog, Cat from the fridge if your use is less than 1 or 2 weeks. (antibody-antibodies.com)
  • Alpha-2-macroglobulin has in its structure a 35 amino acid "bait" region. (wikipedia.org)
  • When High resolution images of rat acute-phase Alpha-2 macroglobulin were performed, studies showed that the comparison of the amino acid compositions of Alpha-2 macroglobulin from Rat and Alpha-2 macroglobulin from human indicated a high degree of homology between the two molecules. (innov-research.com)
  • Alpha-2-Macroglobulin, also known as α2-macroglobulin and abbreviated as α2M and A2M, is a tetrameric glycoprotein produced in the liver and found in blood. (bio-rad-antibodies.com)
  • identification of a 420-kD cell surface glycoprotein specific for the activated conformation of α 2 -macroglobulin. (springer.com)
  • We offer alpha 2-Macroglobulin Lysates for use in common research applications: Western Blot. (novusbio.com)
  • Alpha 2 macroglobulin activity in rats infected with Typanosoma lewisi and treated with cyclophosphamide and its effect on the malignancy of the disease. (bvsalud.org)
  • Some experts in the field of regenerative medicine question the need to concentrate alpha 2 macroglobulin stating that blood plasma has enough alpha 2 macroglobulin to shut down the degrading cartilage enzymes without the need to concentrate it. (livelongstayyoung.com)
  • The study was performed to evaluate whether targeted alpha-2-macroglobulin (A2M) variants have a similar or enhanced function at wild-type (wt)-A2M to attenuate cartilage degeneration in vivo. (biomedcentral.com)
  • Wild-type alpha-2-macroglobulin ( WT-A2M ) ( left ) and A2M variants CYT-98 and CYT-108 ( right ) inhibit cartilage catabolism induced by TNFα and IL-1β. (biomedcentral.com)
  • 1989. α 2-Macroglobulin traps a proteinase in the midregion of its arms. (springer.com)
  • Crookston KP, Webb DJ, Wolf BB, Gonias SLJ (1994) Classification of alpha 2-macroglobulin-cytokine interactions based on affinity of noncovalent association in solution under apparent equilibrium conditions. (springer.com)
  • The resulting anti-human alpha-2 Macroglobulin (a2M) was solid phase absorbed then affinity purified off an antigen containing immunoabsorbant. (leebio.com)
  • A common variant (29.5%) (polymorphism) of alpha-2-macroglobulin leads to increased risk of Alzheimer's disease. (wikipedia.org)
  • No specific deficiency with associated disease has been recognized, and no disease state is attributed to low concentrations of alpha-2-macroglobulin. (wikipedia.org)
  • Human fibroblast collagenase-alpha-macroglobulin interactions. (wikipedia.org)
  • Alpha 2 macroglobulin shuts down the destructive processes that leads to joint degeneration and PRP provides the growth factors for repair. (livelongstayyoung.com)
  • Alpha 2-macroglobulin can suppress TNF-a-induced activation of NF-?B signaling pathway in CEP cells, thus alleviating the degeneration of IVD. (lww.com)