alpha-L-Fucosidase
Fucosidosis
An autosomal recessive lysosomal storage disease caused by a deficiency of ALPHA-L-FUCOSIDASE activity resulting in an accumulation of fucose containing SPHINGOLIPIDS; GLYCOPROTEINS, and mucopolysaccharides (GLYCOSAMINOGLYCANS) in lysosomes. The infantile form (type I) features psychomotor deterioration, MUSCLE SPASTICITY, coarse facial features, growth retardation, skeletal abnormalities, visceromegaly, SEIZURES, recurrent infections, and MACROGLOSSIA, with death occurring in the first decade of life. Juvenile fucosidosis (type II) is the more common variant and features a slowly progressive decline in neurologic function and angiokeratoma corporis diffusum. Type II survival may be through the fourth decade of life. (From Menkes, Textbook of Child Neurology, 5th ed, p87; Am J Med Genet 1991 Jan;38(1):111-31)
Lymphocyte Function-Associated Antigen-1
An integrin heterodimer widely expressed on cells of hematopoietic origin. CD11A ANTIGEN comprises the alpha chain and the CD18 antigen (ANTIGENS, CD18) the beta chain. Lymphocyte function-associated antigen-1 is a major receptor of T-CELLS; B-CELLS; and GRANULOCYTES. It mediates the leukocyte adhesion reactions underlying cytolytic conjugate formation, helper T-cell interactions, and antibody-dependent killing by NATURAL KILLER CELLS and granulocytes. Intracellular adhesion molecule-1 has been defined as a ligand for lymphocyte function-associated antigen-1.
Antigens, CD18
Integrins
A family of transmembrane glycoproteins (MEMBRANE GLYCOPROTEINS) consisting of noncovalent heterodimers. They interact with a wide variety of ligands including EXTRACELLULAR MATRIX PROTEINS; COMPLEMENT, and other cells, while their intracellular domains interact with the CYTOSKELETON. The integrins consist of at least three identified families: the cytoadhesin receptors(RECEPTORS, CYTOADHESIN), the leukocyte adhesion receptors (RECEPTORS, LEUKOCYTE ADHESION), and the VERY LATE ANTIGEN RECEPTORS. Each family contains a common beta-subunit (INTEGRIN BETA CHAINS) combined with one or more distinct alpha-subunits (INTEGRIN ALPHA CHAINS). These receptors participate in cell-matrix and cell-cell adhesion in many physiologically important processes, including embryological development; HEMOSTASIS; THROMBOSIS; WOUND HEALING; immune and nonimmune defense mechanisms; and oncogenic transformation.
Integrin alpha4beta1
Integrin alpha4beta1 is a FIBRONECTIN and VCAM-1 receptor present on LYMPHOCYTES; MONOCYTES; EOSINOPHILS; NK CELLS and thymocytes. It is involved in both cell-cell and cell- EXTRACELLULAR MATRIX adhesion and plays a role in INFLAMMATION, hematopoietic cell homing and immune function, and has been implicated in skeletal MYOGENESIS; NEURAL CREST migration and proliferation, lymphocyte maturation and morphogenesis of the PLACENTA and HEART.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Amino Acid Sequence
Base Sequence
alpha 1-Antitrypsin
Fluorescent Dyes
Octopodiformes
Isoelectric Focusing
Crystallography, X-Ray
Catalysis
Methylmalonate-Semialdehyde Dehydrogenase (Acylating)
An enzyme that plays a role in the VALINE; LEUCINE; and ISOLEUCINE catabolic pathways by catalyzing the oxidation of 2-methyl-3-oxopropanate to propanoyl-CoA using NAD+ as a coenzyme. Methylmalonate semialdehyde dehydrogenase deficiency is characterized by elevated BETA-ALANINE and 3-hydropropionic acid.
MedlinePlus
Glycolipids
Hydrothermal Vents
Influenza A virus
The type species of the genus INFLUENZAVIRUS A that causes influenza and other diseases in humans and animals. Antigenic variation occurs frequently between strains, allowing classification into subtypes and variants. Transmission is usually by aerosol (human and most non-aquatic hosts) or waterborne (ducks). Infected birds shed the virus in their saliva, nasal secretions, and feces.
Influenza, Human
Influenza A Virus, H1N1 Subtype
Influenza Vaccines
Vaccines used to prevent infection by viruses in the family ORTHOMYXOVIRIDAE. It includes both killed and attenuated vaccines. The composition of the vaccines is changed each year in response to antigenic shifts and changes in prevalence of influenza virus strains. The vaccine is usually bivalent or trivalent, containing one or two INFLUENZAVIRUS A strains and one INFLUENZAVIRUS B strain.
Hemagglutinin Glycoproteins, Influenza Virus
Membrane glycoproteins from influenza viruses which are involved in hemagglutination, virus attachment, and envelope fusion. Fourteen distinct subtypes of HA glycoproteins and nine of NA glycoproteins have been identified from INFLUENZA A VIRUS; no subtypes have been identified for Influenza B or Influenza C viruses.
Influenza in Birds
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Endometrial lysosomal enzyme activity in normal cycling endometrium. (1/168)
The objective of this study was to evaluate the possible role of four lysosomal enzymes in endometrial function and remodelling during the normal menstrual cycle by fluorimetric measurement (acid phosphatase, N-acetyl-beta-D-glucosaminidase, alpha-L-fucosidase and alpha-D-mannosidase). A prospective study was conducted of 45 endometrial biopsies obtained from women with normal menstrual cycles. Activity of all four enzymes was identified in human endometrium. Activity of acid phosphatase and N-acetyl-beta-D-glucosaminidase was relatively high, whilst that of alpha-L-fucosidase and alpha-D-mannosidase was low. There was no significant change in the activity of any of the four enzymes from the proliferative to the secretory phase of the cycle. This study suggests that the activity of these enzymes remains constant throughout a major portion of the normal cycle. (+info)Characterization of human semen alpha-L-fucosidases. (2/168)
Human semen contains a large amount of alpha-L-fucosidase activity, the great majority of which is found in the seminal fluid. Immunocytochemical studies indicate that a small amount of semen fucosidase activity is present on the sperm plasma membrane, primarily in the posterior head region. Subcellular fractionation studies also indicate that sperm alpha-L-fucosidase is present in the plasma membrane-enriched fraction. Comparative characterization of human seminal fluid and sperm alpha-L-fucosidases indicates that seminal fluid alpha-L-fucosidase has a broad pH optimum curve with a number of near-equal maxima between pH 4.8 and 7.0 while sperm fucosidase has a major optimum between pH 3.4 and 4.0. Isoelectric focusing indicates that seminal fluid alpha-L-fucosidase contains three to six isoforms with isoelectric points (pI) of 5-7 while sperm fucosidase contains two distinct isoforms with pI values of 5. 2 +/- 0.2 and 7.0 +/- 0.2. Western blotting indicates that seminal fluid fucosidase contains a major protein band with a molecular mass ratio (M(r)) of approximately 56 kDa while sperm fucosidase contains a major protein band of approximately 51 kDa. The overall results indicate the presence of a low-abundance, plasma membrane-associated human sperm alpha-L-fucosidase, which is different in its properties from human seminal fluid alpha-L-fucosidase(s), and whose function is not yet known. (+info)Fucose in alpha(1-6)-linkage regulates proliferation and histogenesis in reaggregated retinal spheroids of the chick embryo. (3/168)
We have used the lectin from Aleuria aurantia (AAL) which is highly specific for alpha(1-6)-linked fucose, to examine its effect on chicken retinogenesis in a reaggregation culture system. When dispersed cells of the embryonic chick retina are reaggregated to form histotypic retinospheroids, AAL elicits strong inhibition of spheroid growth. The action of AAL is specific, since its effect is dose-dependent, saturable, and inhibited by an excess of fucose. Fucosidase treatment entirely abolishes reaggregation. In contrast, Anguilla anguilla agglutinin (AAA) binding to fucose in alpha(1-2)-linkage does not show any effects. Incubation with CAB4-a specific monoclonal antibody for fucose in alpha(1-6)-linkage-reduces spheroid size and shape. AAL does not much affect primary aggregation, but rather subsequent processes of cell proliferation and histogenesis. In particular, AAL inhibits uptake of bromo-desoxyuridine (BrdU), most efficiently so during days in vitro 2 (div2) and div3. As a consequence, the histological differentiation is entirely disturbed, as evidenced by vimentin immunostaining; particularly, rosettes are not forming and the radial glia scaffold is disorganized. We conclude that glycoproteins exhibiting fucose in alpha(1-6)-linkage may play major roles in early processes of retinal tissue formation. (+info)Glycosylation alterations of cells in late phase apoptosis from colon carcinomas. (4/168)
Comparisons of carbohydrate profiles between control and apoptotic colon carcinoma cells were performed by flow cytometry using a set of lectins and anti-carbohydrate antibodies. The six cell lines analyzed presented distinct carbohydrate profiles before induction of apoptosis. PHA-L and MAA binding decreased after induction of apoptosis by UV-treatment. In contrast an increase of PNA binding was observed after induction of apoptosis, except on SW-48 cells for which a decrease occurred. A decrease of SNA binding was observed after induction of apoptosis from strongly positive control cell lines, whereas it increased on weakly positive ones. All the blood group related antigens A, H, Lewis a, Lewis x, Lewis b, and Lewis y, had their expression strongly diminished on apoptotic cells. These changes occurred irrespective of the mode of apoptosis induction since similar results were obtained after UV, TNFalpha, or anti-Fas treatment. Fucosyltransferases activities were also decreased after apoptosis induction, except for alpha1,3fucosyltransferase in anti-Fas treated HT-29 cells, where it was strongly augmented. This could be attributed to the IFNgamma preteatment required to induce Fas expression on these cells. Fucosidase activity decreased after induction of apoptosis suggesting that it was not responsible for the loss of fucosylated structures. In the rat PRO cell line, H blood group antigens are mainly carried by a high molecular weight variant of CD44. It could be shown that the loss of H antigen after induction of apoptosis correlated with a loss of the carrier glycoprotein. (+info)Terminal glycosylation in cystic fibrosis. (5/168)
Cystic fibrosis (CF) is a common genetic disease for which the gene was identified within the last decade. Pulmonary disease predominates in this ultimately fatal disease and current therapy only slows the progression. CF transmembrane regulator (CFTR), the gene product, is an integral membrane glycoprotein that normally functions as a chloride channel in epithelial cells. The most common mutation, deltaF508, results in mislocalization and altered glycosylation of CFTR. Altered fucosylation and sialylation are hallmarks of both membrane and secreted glycoproteins in CF and the focus here is on these investigations. Oligosaccharides from CF membrane glycoproteins have the Lewis x, selectin ligand in terminal positions. In addition, two major bacterial pathogens in CF, Pseudomonas aeruginosa and Haemophilus influenzae, have binding proteins, which recognize fucose in alpha1,3 linkage and asialoglycoconjugates. We speculate that the altered terminal glycosylation of airway epithelial glycoproteins in CF contributes to the chronic infection and robust inflammatory response in the CF lung. Understanding the effects of mutant CFTR on glycosylation may provide further insight into the regulation of glycoconjugate processing as well as therapy for CF. (+info)Prediction of the development of hepato-cellular-carcinoma in patients with liver cirrhosis by the serial determinations of serum alpha-L-fucosidase activity. (6/168)
OBJECTIVE: Evaluation of the usefulness of the serial determinations of serum alpha-L-fucosidase (AFU) activity for prediction of the development of hepatocellular carcinoma (HCC) was performed. METHODS AND PATIENTS: Serum AFU activity was determined monthly for 42 months in 73 patients with liver cirrhosis (LC). RESULTS: HCC was diagnosed in 27 patients by means of ultrasonography during this observation period. In 23 (85%) of the 27 patients, serum AFU activity was found to exceed 700 nmole/ml/h during the LC stage. HCC developed within a few years in 23 (82%) of 28 LC patients with AFU activity exceeding 700 nmole/ml/h, in contrast, it developed in only 4 (9%) of 45 LC patients with AFU activity below 700 nmole/ml/h. AFU activity was already elevated in 23 (85%) of 27 patients at least 6 months before the detection of HCC by ultrasonography. CONCLUSION: It is conceivable that the development of HCC can be predicted by means of serial determinations of serum AFU activity in patients with LC. (+info)Endometrial lysosomal enzyme activity in ovulatory dysfunctional uterine bleeding, IUCD users and post-partum women. (7/168)
The aim of this study was to evaluate the role of lysosomal enzymes in excessively heavy menstruation by comparing women with menorrhagia due to dysfunctional bleeding or intrauterine contraceptive device (IUCD) use with those with normal menstrual periods or with amenorrhoea associated with breastfeeding. This was a prospective cohort investigation of the activity of four endometrial lysosomal enzymes in three contrasting groups: (i) women with ovulatory dysfunctional uterine bleeding and users of intrauterine contraceptive devices; (ii) breastfeeding post-partum women in whom there are long periods of amenorrhoea, particularly in the early months post-partum; and (iii) normal cycling women. It was found that the total activity of lysosomal enzymes, particularly acid phosphatase and N-acetyl-beta-D-glucosaminidase, was markedly elevated (P < 0.001) in IUCD-exposed endometrium, and endometrium from women with dysfunctional uterine bleeding when compared with endometrium from women with a history of entirely normal menstrual periods or that in post-partum breastfeeding women. The activity of alpha-L-fucosidase was moderately elevated in IUCD users (P < 0.05) and ovulatory dysfunctional uterine bleeding (P < 0.05), whereas alphaD-mannosidase activity was elevated in ovulatory dysfunctional uterine bleeding (P < 0.05), but decreased in IUCD users (P < 0.01). No significant differences were observed in the lysosomal enzyme activities of breastfeeding post-partum women and normal cycling women. These results show that total endometrial tissue activity of four lysosomal enzymes was substantially increased throughout the cycle in most circumstances in women with two different causes for increased menstrual bleeding. This suggests a contributory role to the increased bleeding. (+info)Sequence and expression of Thai Rosewood beta-glucosidase/beta-fucosidase, a family 1 glycosyl hydrolase glycoprotein. (8/168)
Dalcochinin-8'-O-beta-glucoside beta-glucosidase (dalcochinase) from the Thai rosewood (Dalbergia cochinchinensis Pierre) has aglycone specificity for isoflavonoids and can hydrolyze both beta-glucosides and beta-fucosides. To determine its structure and evolutionary lineage, the sequence of the enzyme was determined by peptide sequencing followed by PCR cloning. The cDNA included a reading frame coding for 547 amino acids including a 23 amino acid propeptide and a 524 amino acid mature protein. The sequences determined at peptide level were found in the cDNA sequence, indicating the sequence obtained was indeed the dalcochinase enzyme. The mature enzyme is 60% identical to the cyanogenic beta-glucosidase from white clover glycosyl hydrolase family 1, for which an X-ray crystal structure has been solved. Based on this homology, residues which may contribute to the different substrate specificities of the two enzymes were identified. Eight putative glycosylation sites were identified, and one was confirmed to be glycosylated by Edman degradation and mass spectrometry. The protein was expressed as a prepro-alpha-mating factor fusion in Pichia pastoris, and the activity of the secreted enzyme was characterized. The recombinant enzyme and the enzyme purified from seeds showed the same K(m) for pNP-glucoside and pNP-fucoside, had the same ratio of V(max) for these substrates, and similarly hydrolyzed the natural substrate, dalcochinin-8'-beta-glucoside. (+info)
1 3 alpha l fucosidase : définition de 1 3 alpha l fucosidase et synonymes de 1 3 alpha l fucosidase (anglais)
An anthracenecarboximide fluorescent probe for in vitro and in vivo ratiometric imaging of endogenous alpha-l-fucosidase for...
Identifying the Catalytic Acid/Base in GH29 alpha-L-Fucosidase Subfamilies | UBC Chemistry
AID 36841 - Inhibition of the enzyme alpha-L-fucosidase from bovine epididymis was determined at 1 mM concentration of the...
KEGG BRITE: KEGG Orthology (KO) - Streptococcus pneumoniae TIGR4 (virulent serotype 4)
Synthesis and evaluation of a 5-Membered Isoiminosugar as Glycosidase Inhibitor<...
Cell surface associated alpha-L-fucose moieties modulate human breast cancer neoplastic progression
Canser yr afu - Wicipedia
FUCA1P1 Gene - GeneCards | FUCA1P1 Pseudogene
Fucosidases
A Comparative Study of Inductive Influence on the Hydrolysis of Beta-T by Robert W. Miller Jr.
FUCA1 Gene - GeneCards | FUCO Protein | FUCO Antibody
Isoenzymes of human liver α-L-fucosidase: Chemical relationship, kinetic studies, and immunochemical characterization |...
Bacillus sp. ES-SL-2 | Semantic Scholar
CAZy - GH30 3
CAZy - GH30 3
FUCA2 knockout cell line
2IWV | Genus
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Fucosidosis: genetic and biochemical analysis of eight cases. | Journal of Medical Genetics
Adelaide Research & Scholarship: Canine fucosidosis: a model for retroviral gene transfer into haematopoietic stem cells
Structural and enzymatic characterization of a glycoside hydrolase family 31 alpha-xylosidase from <em>Cellvibrio japonicus</em...
A mouse model for fucosidosis recapitulates storage pathology and neurological features of the milder form of the human disease...
Treatment of canine fucosidosis by intracisternal enzyme infusion - Fingerprint
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Synthesis and glycosidase inhibitory activities of 2-(aminoalkyl)pyrrolidine-3,4-diol derivatives
Sperm-associated antigen elisa and antibody
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Glycoside hydrolase family 29
Alpha-L-fucosidase is responsible for hydrolysing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of ... Glycoside hydrolase family 29 includes alpha-L-fucosidases, They are lysosomal enzymes responsible for hydrolyzing the alpha-1, ... making alpha-l-fucosidases, the enzymes responsible for their processing, critically important. Deficiency in alpha-l- ... In human sperm, membrane-associated alpha-l-fucosidase is stable for extended periods of time, which is made possible by ...
FUCA2
Plasma alpha-L-fucosidase (see alpha-L-fucosidase) is an enzyme that in humans is encoded by the FUCA2 gene. GRCh38: Ensembl ... "Entrez Gene: FUCA2 fucosidase, alpha-L- 2, plasma". Narahara K, Tsuji K, Yokoyama Y, et al. (1991). "Specification of small ... Eiberg H, Mohr J, Nielsen LS (Oct 1984). "Linkage of plasma alpha-L-fucosidase (FUCA2) and the plasminogen (PLG) system". Clin ... 2001). "Cell surface human alpha-L-fucosidase". Eur. J. Biochem. 268 (11): 3321-31. doi:10.1046/j.1432-1327.2001.02237.x. PMID ...
Fucosidosis
The gene encoding the alpha-fucosidase, FUCA 1, was found to be located to the short arm of chromosome 1p36 - p34, by Carrit ... If they are present, a skin or blood sample will be taken to test for below-normal amounts of alpha-fucosidase. - Fucosidosis ... The FUCA1 gene provides instructions for making an enzyme called alpha-L-fucosidase. The enzyme plays a role in the breakdown ... The deficiency of the enzyme alpha-L-fucosidase, which is used to metabolize complex compounds in the body (fucose-containing ...
Thymidine kinase in clinical chemistry
Zhang SY, Lin BD, Li BR (2015). "Evaluation of the diagnostic value of alpha-l-fucosidase, alpha-fetoprotein and thymidine ...
Penicillium multicolor
"An alpha-L-fucosidase from Penicillium multicolor as a candidate enzyme for the synthesis of alpha (1-->3)-linked fucosyl ... Penicillium multicolor is an anamorph species of the genus Penicillium which produces alpha-L-fucosidase, tilactase, ... Ajisaka, K.; Fujimoto, H.; Miyasato, M. (1998). "An α-l-fucosidase from Penicillium multicolor as a candidate enzyme for the ...
Macrococcus brunensis
... alpha-galactosidase, N-acetyl-beta-glucosaminidase, alpha-mannosidase, alpha-fucosidase and esterase-lipase (C8) are negative. ... Acid is not produced from arabinose, N-acetylglucosamine, D-cellobiose, D-galactose, or methyl alpha-D-glucoside, lactose, D- ...
Fucoidanase
... alpha-L-fucosidase, poly(1,2-alpha-L-fucoside-4-sulfate) glycanohydrolase) is an enzyme with systematic name poly((1->2)-alpha- ... This enzyme catalyses the following chemical reaction Endohydrolysis of (1->2)-alpha-L-fucoside linkages in fucoidan without ...
List of EC numbers (EC 3)
2-alpha-L-fucosidase EC 3.2.1.64: 2,6-b-fructan 6-levanbiohydrolase EC 3.2.1.65: levanase EC 3.2.1.66: quercitrinase EC 3.2. ... 3-alpha-L-fucosidase EC 3.2.1.112: 2-deoxyglucosidase EC 3.2.1.113: mannosyl-oligosaccharide 1,2-a-mannosidase EC 3.2.1.114: ... 6-alpha-L-fucosidase EC 3.2.1.128: glycyrrhizinate beta-glucuronidase EC 3.2.1.129: endo-a-sialidase EC 3.2.1.130: glycoprotein ... alpha-agarase EC 3.2.1.159: alpha-neoagaro-oligosaccharide hydrolase EC 3.2.1.160: now EC 3.2.1.155 EC 3.2.1.161: beta-apiosyl- ...
Fucosidase
Tissue alpha-L-fucosidase is an enzyme that in humans is encoded by the FUCA1 gene. Alpha-Fucosidase is an enzyme that breaks ... "Entrez Gene: FUCA1 fucosidase, alpha-L- 1, tissue". HPRD entry [1] Willems PJ, Gatti R, Darby JK, et al. (1991). "Fucosidosis ... Fukushima H, de Wet JR, O'Brien JS (1985). "Molecular cloning of a cDNA for human alpha-L-fucosidase". Proc. Natl. Acad. Sci. U ... Yang M, Allen H, DiCioccio RA (1993). "A mutation generating a stop codon in the alpha-L-fucosidase gene of a fucosidosis ...
Alpha-fucosidase
... may refer to one of two enzymes: Fucosidase Alpha-L-fucosidase This set index page lists enzyme articles ...
Alpha-L-fucosidase
1,2-alpha-L-fucosidase 1,3-alpha-L-fucosidase 1,6-alpha-L-fucosidase FUCA2 Endreffy I, Bjørklund G, Szerafin L, Urbina MA, ... In enzymology, an alpha-L-fucosidase (EC 3.2.1.51) is an enzyme that catalyzes the chemical reaction an alpha-L-fucoside + H2O ... The systematic name of this enzyme class is alpha-L-fucoside fucohydrolase. This enzyme is also called alpha-fucosidase. This ... doi: 10.1007/s12026-017-8943-x. Levvy GA, Mcallan A (August 1961). "Mammalian fucosidases. 2. alpha-L-Fucosidase". The ...
List of MeSH codes (D08)
... alpha-L-fucosidase MeSH D08.811.277.450.410 - galactosidases MeSH D08.811.277.450.410.050 - alpha-galactosidase MeSH D08.811. ... gtp-binding protein alpha subunits MeSH D08.811.277.040.330.300.200.100.100 - gtp-binding protein alpha subunits, g12-g13 MeSH ... gtp-binding protein alpha subunit, gi2 MeSH D08.811.277.040.330.300.200.100.300 - gtp-binding protein alpha subunits, gq-g11 ... steroid 12-alpha-hydroxylase MeSH D08.811.682.690.708.170.915.737 - steroid 16-alpha-hydroxylase MeSH D08.811.682.690.708.170. ...
1,2-alpha-L-fucosidase
In enzymology, a 1,2-alpha-L-fucosidase (EC 3.2.1.63) is an enzyme that catalyzes the chemical reaction methyl-2-alpha-L- ... Other names in common use include almond emulsin fucosidase, and alpha-(1->2)-L-fucosidase. As of late 2007, 4 structures have ... 2-alpha-L-fucosidase". J. Biol. Chem. 245 (2): 299-304. PMID 5460888. Ogata-Arakawa M, Muramatsu T, Kobata A (1977). "alpha-L- ... Purification and properties of alpha-L-fucosidase from Chamelea gallina L". Eur. J. Biochem. 66 (2): 379-87. doi:10.1111/j.1432 ...
1,3-alpha-L-fucosidase
In enzymology, a 1,3-alpha-L-fucosidase (EC 3.2.1.111) is an enzyme that catalyzes the chemical reaction of cleaving the 1,3- ... Yoshima H, Takasaki S, Ito-Mega S, Kobata A (1979). "Purification of almond emulsin alpha-L-fucosidase I by affinity ... Ogata-Arakawa M, Muramatsu T, Kobata A (1977). "alpha-L-fucosidases from almond emulsin: characterization of the two enzymes ... The systematic name of this enzyme class is 3-alpha-L-fucosyl-N-acetylglucosaminyl-glycoprotein fucohydrolase. This enzyme is ...
1,6-alpha-L-fucosidase
This enzyme is also called alpha-L-fucosidase. Yazawa S, Madiyalakan R, Chawda RP, Matta KL (1986). "alpha-L-fucosidase from ... 6-alpha-L-fucosidase (EC 3.2.1.127) is an enzyme that catalyzes the chemical reaction Hydrolysis of 1,6-linkages between alpha- ... aspergillus niger: demonstration of a novel alpha-L-(1----6)-fucosidase acting on glycopeptides". Biochem. Biophys. Res. Commun ...
Pseudogymnoascus destructans
... alpha/beta-galactosidase, β-glucuronidase, α-fucosidase, α-mannosidase, and trypsin. Important dual virulence factors found in ...
Acid alpha-glucosidase
alpha-1,4-glucosidase activity. • alpha-glucosidase activity. Cellular component. • membrane. • lysosomal membrane. • lysosomal ... Acid alpha-glucosidase, also called α-1,4-glucosidase[5] and acid maltase,[6] is an enzyme (EC 3.2.1.20) that helps to break ... maltose alpha-glucosidase activity. • catalytic activity. • hydrolase activity. • carbohydrate binding. • isomaltase. • ... This gene encodes lysosomal alpha-glucosidase, which is essential for the degradation of glycogen to glucose in lysosomes. ...
Beta-D-fucosidase
... beta-D-glucosides and alpha-L-arabinosides. Chinchetru MA, Cabezas JA, Calvo P (1983). "Characterization and kinetics of beta-D ... Beta-D-fucosidase (EC 3.2.1.38, beta-fucosidase) is an enzyme with systematic name beta-D-fucoside fucohydrolase. This enzyme ... Beta-D-fucosidase at the US National Library of Medicine Medical Subject Headings (MeSH) Biology portal. ... Wiederschain GY, Prokopenkov AA (October 1973). "Beta-D-galactosidase and beta-D-fucosidase of pig kidney". Archives of ...
Mannan endo-1,6-alpha-mannosidase
... (EC 3.2.1.101, exo-1,6-beta-mannanase, endo-alpha-1-,6-D-mannanase, endo-1,6-beta-mannanase, ... mannan endo-1,6-beta-mannosidase, 1,6-alpha-D-mannan mannanohydrolase) is an enzyme with systematic name 6-alpha-D-mannan ... Random hydrolysis of (1-,6)-alpha-D-mannosidic linkages in unbranched (1-,6)-mannans. References[edit]. *^ Nakajima, T.; Maitra ... Mannan endo-1,6-alpha-mannosidase at the US National Library of Medicine Medical Subject Headings (MeSH) ...
Beta-glucosidase
Alpha-N-acetylgalactosaminidase *NAGA. *Alpha-N-acetylglucosaminidase. *Fucosidase. *Hexosaminidase *HEXA. *HEXB. *Iduronidase ...
Cellulase
McCleary BV (November 1980). "New chromogenic substrates for the assay of alpha-amylase and (1 leads to 4)-beta-D-glucanase". ... by the species Coprinopsis Cinerea consists of seven protein strands in the shape of an enclosed tunnel called a beta/alpha ...
Sucrase
EC 3.2.1.48 is sucrose alpha-glucosidase. Physiology[edit]. Sucrose intolerance (also known as congenital sucrase-isomaltase ...
Neuraminidase
Alpha-N-acetylgalactosaminidase *NAGA. *Alpha-N-acetylglucosaminidase. *Fucosidase. *Hexosaminidase *HEXA. *HEXB. *Iduronidase ...
Amylase
Main article: Alpha-amylase. The α-amylases (EC 3.2.1.1 ) (CAS 9014-71-5) (alternative names: 1,4-α-D-glucan glucanohydrolase; ... An inhibitor of alpha-amylase, called phaseolamin, has been tested as a potential diet aid.[10] ... Park HS, Kim HY, Suh YJ, Lee SJ, Lee SK, Kim SS, Nahm DH (September 2002). "Alpha amylase is a major allergenic component in ... Like in other mammals, the pancreatic alpha-amylase AMY2 was duplicated multiple times. One event allowed it to evolve salivary ...
Glycoside hydrolase
For example, a combination of alpha-amylase and cellulase was shown to degrade polymicrobial bacterial biofilms from both in ...
Oligosaccharyltransferase
OST is a component of the translocon in the endoplasmic reticulum (ER) membrane. A lipid-linked core-oligosaccharide is assembled at the membrane of the endoplasmic reticulum and transferred to selected asparagine residues of nascent polypeptide chains by the oligosaccharyl transferase complex.[3] The active site of OST is located about 4 nm from the lumenal face of the ER membrane.[4] It usually acts during translation as the nascent protein is entering the ER, but this cotranslational glycosylation is nevertheless called a posttranslational modification. A few examples have been found of OST activity after translation is complete.[5][6] Current opinion is that post-translational activity may occur if the protein is poorly folded or folds slowly.[6] ...
Glycoprotein
Alpha-fetoprotein. *Erythropoietin (EPO). Functions[edit]. Some functions served by glycoproteins[5]:524 Function Glycoproteins ...
Alpha-fucosidase - Wikipedia
Alpha-L-fucosidase - Wikipedia
1,2-alpha-L-fucosidase 1,3-alpha-L-fucosidase 1,6-alpha-L-fucosidase FUCA2 Endreffy I, Bjørklund G, Szerafin L, Urbina MA, ... In enzymology, an alpha-L-fucosidase (EC 3.2.1.51) is an enzyme that catalyzes the chemical reaction an alpha-L-fucoside + H2O ... The systematic name of this enzyme class is alpha-L-fucoside fucohydrolase. This enzyme is also called alpha-fucosidase. This ... doi: 10.1007/s12026-017-8943-x. Levvy GA, Mcallan A (August 1961). "Mammalian fucosidases. 2. alpha-L-Fucosidase". The ...
Alpha-fucosidase | definition of alpha-fucosidase by Medical dictionary
... alpha-fucosidase explanation free. What is alpha-fucosidase? Meaning of alpha-fucosidase medical term. What does alpha- ... Looking for online definition of alpha-fucosidase in the Medical Dictionary? ... Alpha-fucosidase , definition of alpha-fucosidase by Medical dictionary https://medical-dictionary.thefreedictionary.com/alpha- ... medical-dictionary.thefreedictionary.com/alpha-fucosidase,alpha-fucosidase,/a,. *Facebook ...
Sequence Similarity
- 1HL8: CRYSTAL STRUCTURE OF THERMOTOGA MARITIMA ALPHA-FUCOSIDASE Sequence Similarity Report Page
Crystal Structure of Thermotoga Maritima Alpha-L-Fucosidase. Insights Into the Catalytic Mechanism and the Molecular Basis for ... Alpha-L-fucosidase, putative 2336 3.2.1.51 , Details 4 1HL9 1 A, B PUTATIVE ALPHA-L-FUCOSIDASE ORF TM0306 2336 3.2.1.51 , ... Alpha-L-fucosidase, putative 2336 3.2.1.51 , Details 7 1HL8 1 A, B PUTATIVE ALPHA-L-FUCOSIDASE ORF TM0306 2336 3.2.1.51 , ... ALPHA-L-FUCOSIDASE, PUTATIVE 2336 3.2.1.51 , Details 14 1ODU 1 A, B PUTATIVE ALPHA-L-FUCOSIDASE ORF TM0306 2336 3.2.1.51 , ...
AID 453109 - Inhibition of bovine kidney alpha-L-fucosidase by para-nitrophenolate release assay - PubChem
Human Metabolome Database: Showing Protein Plasma alpha-L-fucosidase (HMDBP00876)
Sequence Similarity
- 1HL9: CRYSTAL STRUCTURE OF THERMOTOGA MARITIMA ALPHA-FUCOSIDASE IN COMPLEX WITH A MECHANISM...
L-Fucosidase: Insights Into the Catalytic Mechanism and the Molecular Basis for Fucosidosis ... PUTATIVE ALPHA-L-FUCOSIDASE protein, length: 449 (BLAST) Sequence Similarity Cutoff. Rank. Chains in Cluster. Cluster ID / Name ... CRYSTAL STRUCTURE OF THERMOTOGA MARITIMA ALPHA-FUCOSIDASE IN COMPLEX WITH A MECHANISM BASED INHIBITOR. ...
An anthracenecarboximide fluorescent probe for in vitro and in vivo ratiometric imaging of endogenous alpha-l-fucosidase for...
Herein, a ratiometric fluorescent probe for the alpha-l-fucosidase (AFU) assay based on a single fluorophore has been developed ... Alpha-l-fucosidase (AFU) plays vital roles in some physiological processes that closely relate to several diseases. ... Alpha-L-fucosidase (AFU) plays vital roles in some physiological processes that closely relate to several diseases. Herein, a ... An anthracenecarboximide fluorescent probe for in vitro and in vivo ratiometric imaging of endogenous alpha-L-fucosidase for ...
Alpha-fucosidase elisa and antibody
Recombinant Protein and Alpha-fucosidase Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are ... Alpha-fucosidase A. Alpha-fucosidase A ELISA Kit. Alpha-fucosidase A Recombinant. Alpha-fucosidase A Antibody. Also known as ... Alpha-fucosidase A (Alpha-L-fucoside fucohydrolase A).. Alpha-fucosidase involved in degradation of fucosylated xyloglucans. ... Alpha-fucosidase involved in degradation of fucosylated xyloglucans. Hydrolyzes alpha-1,2-linked fucose. Active on cotton ...
Identifying the Catalytic Acid/Base in GH29 alpha-L-Fucosidase Subfamilies | UBC Chemistry
AID 488425 - Inhibition of bovine epididymis alpha-L-fucosidase assessed as p-nitrophenol release at 1000 uM at pH 5.5 by...
Probable alpha-fucosidase elisa and antibody
Recombinant Protein and Probable alpha-fucosidase Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody ... Probable alpha-fucosidase A. Probable alpha-fucosidase A ELISA Kit. Probable alpha-fucosidase A Recombinant. Probable alpha- ... fucosidase A Antibody. Also known as Probable alpha-fucosidase A (Alpha-L-fucoside fucohydrolase A).. Alpha-fucosidase involved ... Hydrolyzes alpha-1,2-linked fucose (By similarity).. Below are the list of possible Probable alpha-fucosidase products. If you ...
Anti-alpha-L-Fucosidase, 01-20151 | ARP American Research Products, Inc.
alpha-1-2-3-4-6-l-fucosidase enzyme | Megazyme
L-Fucosidase (Homo sapiens) for use in research biochemical enzyme assays and in vitro diagnostic analysis. ... α-(1-2,3,4,6)-L-Fucosidase (Homo sapiens) Compare Favourites Print Email ... One Unit of α-L-fucosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per ... High purity α-(1-2,3,4,6)-L-Fucosidase (Homo sapiens) for use in research, biochemical enzyme assays and in vitro diagnostic ...
PDF] Alteration of the isoform composition of plasma-membrane-associated rat sperm alpha-L-fucosidase during late epididymal...
... rat sperm alpha-L-fucosidase was characterized during epididymal maturation. The pH 7 activity optimum of alpha-L-fucosidase ... alpha-L-Fucosidase from sperm from the distal half of the cauda, which contained a significant enrichment of sperm and alpha-L- ... Isoelectric focusing of alpha-L-fucosidase indicated the presence of a major isoform (B) with a pI near 7 in sperm from testis ... Neuraminidase treatment of sperm alpha-L-fucosidase from distal cauda (when compared with the appropriate heat-treated control ...
Molecular cloning and characterization of Bifidobacterium bifidum 1,2-alpha-L-fucosidase (AfcA), a novel inverting glycosidase ...
2-alpha-l-fucosidase (AfcA) was isolated. The afcA gene was found to comprise 1,959 amino acid residues with a predicted ... A domain responsible for fucosidase activity (the Fuc domain; amino acid residues 577 to 1474) was localized by deletion ... The recombinant Fuc domain specifically hydrolyzed the terminal alpha-(1--,2)-fucosidic linkages of various oligosaccharides ... genomic library of Bifidobacterium bifidum constructed in Escherichia coli was screened for the ability to hydrolyze the alpha ...
N-glycan degradation: Alpha 1,2-L Fucosidase
2-L Fucosidase specifically cleaves the alpha 1,2 fucoside bond, liberating L-fucose from 22-alpha-L-fucosyl lactose (Fuc ... N-glycan degradation: Alpha 1,2-L Fucosidase Alpha 1,2-L Fucosidase specifically cleaves the alpha 1,2 fucoside bond, ... Substrate specificity of alpha 1,2-L-fucosidase (Cat.# 4451). Substrate specificity of alpha 1,2-L-fucosidase (Cat.# 4451). ... Alpha 1,2-L-Fucosidase. 2 Units. USD $402.00 Intended usage: Specific cleavage of α1,2-L-fucoside bonds. This enzyme releases L ...
Alpha-Fucosidase, Leukocytes -
Mayo Clinic Laboratories | Neurology Catalog
Fucosidosis is an autosomal recessive lysosomal storage disorder caused by reduced or absent alpha-L-fucosidase enzyme activity ... enzyme analysis of alpha-L-fucosidase can confirm the diagnosis. ... Alpha-Fucosidase, Leukocytes Reporting Name. Alpha-Fucosidase, ... 3. Thomas GH: Disorders of Glycoprotein Degradation: alpha-Mannosidosis, beta-Mannosidosis, Fucosidosis, and Sialidosis. In The ...
Fucosidase, alpha-L- 1, Tissue (FUCA1) Kits ELISA
alpha-L-fucosidase 1 , alpha-L-fucosidase I , alpha-L-fucoside fucohydrolase 1 , tissue alpha-L-fucosidase , fucosidase, alpha- ... L- 1, tissue , fucosidase, alpha-L-1, tissue , Fucosidase alpha-L-1 tissue , Fucosidase, alpha-L-1, tissue , tissue alpha-L- ... Target Fucosidase, alpha-L- 1, Tissue (FUCA1) * Fucosidase, alpha-L- 1, Tissue (FUCA1) ... Fucosidase, alpha-L- 1, Tissue Kits ELISA 23 Fucosidase, alpha-L- 1, Tissue (FUCA1) Kits ELISA de 9 fabricants sont disponibles ...
Recombinant Fucosidase Alpha L1, Tissue (FUCa1), Cat#RPU41531 - Biomatik
Recombinant Fucosidase Alpha L1, Tissue (FUCa1), Cat#RPU41531. Write a Review Write a Review. × ... Recombinant Fucosidase Alpha L1, Tissue (FUCa1), Cat#RPU41531. Rating Required Select Rating. 1 star (worst). 2 stars. 3 stars ... Fucosidase Alpha L1, Tissue. Alternative Names. A-FU; aFU; Alpha-L-Fucosidase; Alpha-L-fucoside fucohydrolase 1 ...
FUCA1 gene - Genetics Home Reference - NIH
OMIM: FUCOSIDASE, ALPHA-L, 1. *. Willems PJ, Gatti R, Darby JK, Romeo G, Durand P, Dumon JE, OBrien JS. Fucosidosis revisited ... alpha-L-fucosidase 1. Enable Javascript to view the expand/collapse boxes.. Printable PDF Open All Close All ... The FUCA1 gene provides instructions for making an enzyme called alpha-L-fucosidase. This enzyme is found in lysosomes, which ... Most of these mutations result in an abnormally short, nonfunctional alpha-L-fucosidase enzyme. Without this enzyme, ...
An alpha-L-fucosidase from Thermus sp. with unusually broad sp...
with unusually broad specificity.: An alpha-L-fucosidase (E.C. 3.2.1.51) exhibiting a wide aglycon specificity expressed ... The alpha-L-fucosidase hydrolyzes p-nitrophenyl alpha-L-fucopyranoside with V(max) of 12.0 +/- 0.1 microM/min/mg and K(m) = ... An alpha-L-fucosidase from Thermus sp. with unusually broad specificity.. Authors * Eneyskaya, E V ... An alpha-L-fucosidase (E.C. 3.2.1.51) exhibiting a wide aglycon specificity expressed in ability of cleaving alpha1 --, 6-, ...
REAGENTS AND KITS FOR DETECTION OF INFLUENZA VIRUS AND THE LIKE - LI, Xing-xiang
... an alpha-L-Fucosidase substrate (e.g., alpha-L-fucopyranoside-firefly luciferin conjugate) for detection of alpha-L-Fucosidase ... Chemiluminescent alpha-L-fucosidase (AFU) Assays The alpha-L-fucosidase (AFU) assay is for the determination of AFU activity in ... an alpha-L-fucosidase assay using an alpha-L-fucopyranoside-firefly luciferin conjugate as the substrate for detection of alpha ... In a chemiluminescent AFU assay, alpha-L-fucopyranoside-firefly luciferin conjugate is cleaved by alpha-L-fucosidase in a ...
Alpha- l -Fucosidase Isoenzyme iso2 from Paenibacillus thiaminolyticus | BMC Biotechnology | Full Text
Activity-based screening of a genomic library was used to isolate the gene encoding a novel α-L-fucosidase. The enzyme was ... As a very low amino acid sequence similarity with known α-l-fucosidases was found, following study could be important for ... In this study, new enzyme α-l-fucosidase iso2 originating from Paenibacillus thiaminolyticus was described and prepared in ... Hydrolytic and transglycosylation abilities of α-l-fucosidase iso2 were tested using different acceptor molecules. ...
FUCA2 Gene - GeneCards | FUCO2 Protein | FUCO2 Antibody
Alpha-L-Fucosidase 2, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene ... Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of ... Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of ... This gene encodes a plasma alpha-L-fucosidase, which represents 10-20% of the total cellular fucosidase activity. The protein ...
FUCA1 Gene - GeneCards | FUCO Protein | FUCO Antibody
Alpha-L- 1, Tissue, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene ... R&D Systems Antibodies for FUCA1 (Tissue alpha-L-Fucosidase/FUCA1). *R&D Systems Proteins and Enzymes for FUCA1 (Tissue alpha-L ... Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of ... Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of ...
Methods to Validate Mesenchymal Stem Cell Quality: R&D Systems
Alpha-L-Fucosidase. Recently Hildebrand and colleagues identified alpha-L-Fucosidase (alpha-Fuc) as a novel marker of ... 2% of the MSC population must express CD34, CD45, CD11b/Integrin alpha M or CD14, CD79 alpha or CD19, and HLA Class II. ... CD11b/Integrin alpha M, CD14, CD15/Lewis X, CD16/Fc gamma RIII, CD19, CD38, CD56/NCAM-1, CD66b/CEACAM-8, CD123/IL-3 R alpha, ... CD4, CD8 alpha, CD10, CD14a, CD45, CD49b, CD41, CD61, CD62p, CD73, CD90, CD133, HLA-DR. 91. ...
ポピュラーベクター Human 遺伝子 FUCA1P1 (alpha-L-fucosidase 1 pseudogene 1)
FUCA1 gene: MedlinePlus Genetics
The FUCA1 gene provides instructions for making an enzyme called alpha-L-fucosidase. Learn about this gene and related health ... The FUCA1 gene provides instructions for making an enzyme called alpha-L-fucosidase. This enzyme is found in lysosomes, which ... Most of these mutations result in an abnormally short, nonfunctional alpha-L-fucosidase enzyme. Without this enzyme, ... Alpha-L-fucosidase is responsible for cutting (cleaving) off a sugar molecule called fucose toward the end of the breakdown ...
FUCA11.51FucohydrolaseFUCA2TissueFucoseMembrane-associated alpha-L-fucosidaAssayPlasma alpha-L-fucosidaGH29BifidobacteriumActivityEnzymesCharacterizationDegradation of fucosylatedPurificationEncodesHydrolaseCarbohydrateAbstractSpecificityLongumIsoenzymeHomoSubstrateGlycosylThermusAfcAReactionsGlycan structuresCleavage
FUCA16
- 23 Fucosidase, alpha-L- 1, Tissue (FUCA1) Kits ELISA de 9 fabricants sont disponibles sur www.anticorps-enligne.fr. (anticorps-enligne.fr)
- The FUCA1 gene provides instructions for making an enzyme called alpha-L-fucosidase. (nih.gov)
- FUCA1 (Fucosidase, Alpha-L- 1, Tissue) is a Protein Coding gene. (genecards.org)
- At least 2 separate polymorphic alpha-L-fucosidases are recognized in man: that in tissues, FUCA1, which is deficient in fucosidosis (MIM 230000), and that in plasma, FUCA2 (MIM 136820). (thermofisher.com)
- Here, we report the generation of a fucosidosis mouse model, in which the gene for lysosomal α-L-fucosidase ( Fuca1 ) was disrupted by gene targeting. (biologists.org)
- None of the patients had a deletion or gross alteration of the alpha-L-fucosidase gene (FUCA1). (bmj.com)
1.517
- In enzymology, an alpha-L-fucosidase (EC 3.2.1.51) is an enzyme that catalyzes the chemical reaction an alpha-L-fucoside + H2O ⇌ {\displaystyle \rightleftharpoons } L-fucose + an alcohol Thus, the two substrates of this enzyme are alpha-L-fucoside and H2O, whereas its two products are L-fucose and alcohol. (wikipedia.org)
- α- l -Fucosidases (3.2.1.51) are glycosidases that are able to cleave terminal α- l -fucosyl moiety from different types of oligosaccharides and glycoconjugates. (biomedcentral.com)
- Human α-fucosidase (EC 3.2.1.51) is an enzyme (hydrolase) of particular biological and medical interest, as the inherited deficiency in its activity leads to fucosidosis, a pathology belonging to severe glycoprotein lysosomal storage disorders. (elsevier.com)
- α- L -fucosidases (EC 3.2.1.51, FUC), belonging to the glycoside hydrolase family 29 (GH29), play important roles in several biological processes and are markers used for detecting hepatocellular carcinoma. (frontiersin.org)
- O-Glycosyl hydrolases family 29 encompasses alpha-L-fucosidases ( EC 3.2.1.51 ) [ ( PUBMED:2482732 ) ], which is a lysosomal enzyme responsible for hydrolysing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins. (embl-heidelberg.de)
- Alpha-L-fucosidase (EC 3.2.1.51) is a lysosomal enzyme involved in the degradation of fucose-containing glycoproteins and glycolipids (Occhiodoro et al. (thermofisher.com)
- Fucosidosis (OMIM ID 230000) is an ultra-rare neurodegenerative and progressive lysosomal storage disease (LSD) in humans that is caused by a defect in lysosomal α-L-fucosidase [ExPASy enzyme (EC) ID 3.2.1.51] ( Van Hoof and Hers, 1968 ). (biologists.org)
Fucohydrolase2
- The systematic name of this enzyme class is alpha-L-fucoside fucohydrolase. (wikipedia.org)
- Also known as Alpha-fucosidase A (Alpha-L-fucoside fucohydrolase A). Alpha-fucosidase involved in degradation of fucosylated xyloglucans. (mybiosource.com)
FUCA22
- 1,2-alpha-L-fucosidase 1,3-alpha-L-fucosidase 1,6-alpha-L-fucosidase FUCA2 Endreffy I, Bjørklund G, Szerafin L, Urbina MA, Chirumbolo S, Endreffy E. Plasma alpha-L-fucosidase activity in chronic inflammation and autoimmune disorders in a pediatric cohort of hospitalized patients. (wikipedia.org)
- FUCA2 (Alpha-L-Fucosidase 2) is a Protein Coding gene. (genecards.org)
Tissue2
- Study of alpha-L-fucosidase isoenzyme patterns in fetal and maternal tissue. (ac.ke)
- hFUCA1 , which encodes the tissue enzyme, and hFUCA2 , which encodes the plasma α- L -fucosidase. (frontiersin.org)
Fucose18
- Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins. (hmdb.ca)
- Herein, a ratiometric fluorescent probe for the alpha- L -fucosidase (AFU) assay based on a single fluorophore has been developed by functionalizing a new fluorophore platform (6-bromo-anthracenecarboximide) with an α- L -fucose group. (rsc.org)
- Hydrolyzes alpha-1,2-linked fucose. (mybiosource.com)
- Drosophila sperm surface alpha-L-fucosidase interacts with the egg coats through its core fucose residues. (semanticscholar.org)
- Alpha 1,2-L Fucosidase specifically cleaves the alpha 1,2 fucoside bond, liberating L-fucose from 2 2 -alpha-L-fucosyl lactose (Fuc alpha1-2 Gal beta1-4 Glc) and from porcine gastric mucin oligosaccharides. (takarabio.com)
- It also liberates L-fucose from N-acetyllactosamine-type bi-antennary sugar chains with alpha 1,6 fucoside bonds, but the rate of this reaction is extremely slow. (takarabio.com)
- One unit is defined as the amount of enzyme required to hydrolyze 1 µmol of p - nitrophenyl alpha-L-fucoside ( p -NP-Fucose) in 1 minute at 37°C at pH 8.5. (takarabio.com)
- Alpha-L-fucosidase is responsible for cutting (cleaving) off a sugar molecule called fucose toward the end of the breakdown process. (nih.gov)
- The alpha-L-fucosidase hydrolyzes p-nitrophenyl alpha-L-fucopyranoside with V(max) of 12.0 +/- 0.1 microM/min/mg and K(m) = 0.20 +/- 0.05 mM and is able to cleave off about 90% of total L-fucose from pronase-treated fractions of fucosyl-containing glycoproteins and about 30% from the native glycoproteins. (mysciencework.com)
- The protein is a member of the glycosyl hydrolase 29 family, and catalyzes the hydrolysis of the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins. (genecards.org)
- Gene Ontology (GO) annotations related to this gene include fucose binding and alpha-L-fucosidase activity . (genecards.org)
- Fucosidosis is a rare genetic disorder characterized by deficiency of the enzyme alpha-L-fucosidase, which is required to break down (metabolize) certain complex compounds (e.g., fucose-containing glycolipids or fucose-containing glycoproteins). (rarediseases.org)
- Low levels or inactivity of the alpha-L-fucosidase enzyme leads to the abnormal accumulation of fucose-containing compounds in the tissues of individuals with fucosidosis. (rarediseases.org)
- In enzymology , a 1,3-alpha-L-fucosidase ( EC 3.2.1.111 ) is an enzyme that catalyzes the chemical reaction of cleaving the 1,3-linkages between alpha-L- fucose and N-acetyl glucosamine residues in glycoproteins . (leparisien.fr)
- The alpha-1->3 linked core fucose is a suspected carbohydrate antigen for IgE-mediated allergy. (hmdb.ca)
- Fucose is metabolized by an enzyme called alpha-fucosidase. (hmdb.ca)
- One patient in whom an SSCP change was not detected had residual alpha-L-fucosidase activity and cross reacting protein in the heterozygous range and normal metabolism of metabolites containing fucose in his fibroblasts, consistent with the low activity polymorphism. (bmj.com)
- Furthermore, multimeric rCD2 binding to K562 is inhibited by L-fucose and following treatment of K562 with an alpha 1-6 fucosidase, whereas these treatments do not inhibit the binding of CD15 mAb. (jimmunol.org)
Membrane-associated alpha-L-fucosida2
- Stabilization of membrane-associated alpha-L-fucosidase by the human sperm equatorial segment. (semanticscholar.org)
- In human sperm, membrane-associated alpha-l-fucosidase is stable for extended periods of time, which is made possible by membrane domains and compartmentalisation. (embl-heidelberg.de)
Assay4
- If the screening assay is suggestive of fucosidosis, enzyme analysis of alpha-L-fucosidase can confirm the diagnosis. (testcatalog.org)
- The first serologic assay for detection and clinical followup of patients with hepatocellular carcinoma was alpha-fetoprotein (AFP) which has been the standard tumor biomarker for HCC for many years. (hindawi.com)
- Assay of the purified α-l-fucosidase showed only trace amounts of contaminating glycosidases present, with β-galactosidase being the largest contamnant (0.5% by activity). (elsevier.com)
- Assay of the purified serum α-l-fucosidase for sialic acid indicated the presence of 1.7 μg sialic acid per 100 μg enzyme, about twice that previously found for the purified liver enzyme. (elsevier.com)
Plasma alpha-L-fucosida1
- This gene encodes a plasma alpha-L-fucosidase, which represents 10-20% of the total cellular fucosidase activity. (genecards.org)
GH295
- While the catalytic nucleophile in the configuration-retaining alpha-L-fucosidases from family GH29 is fully conserved with respect to sequence, there is no fully sequence-conserved acid/base residue candidate across the family. (ubc.ca)
- Through comparison of kinetic parameters obtained for the hydrolysis of fucosyl substrates with activated leaving groups by these mutants with those of the corresponding wild-type enzymes, in conjunction with the demonstration of azide rescue, we largely confirm the acid/base residue predictions for the GH29 fucosidases from the two subfamilies. (ubc.ca)
- In order to utilize fucosylated oligosaccharides, ATCC 15697 possesses several fucosidases, implicating GH29 and GH95 α- l -fucosidases in a gene cluster dedicated to HMO metabolism. (asm.org)
- Finally, the HMO cluster GH29 α- l -fucosidase possesses a crystal structure that is similar to previously characterized fucosidases. (asm.org)
- The afcB gene encodes a 1493-amino acid polypeptide containing an N-terminal signal sequence, a GH29 - l -fucosidase domain, a carbohydrate binding module (CBM) 32 domain, a found-in-various-architectures (FIVAR) domain and a C-terminal transmembrane region, in this order. (scialert.net)
Bifidobacterium3
- Molecular cloning and characterization of Bifidobacterium bifidum 1,2-alpha-L-fucosidase (AfcA), a novel inverting glycosidase (glycoside hydrolase family 95). (semanticscholar.org)
- Previous research conducted on bifidobacterial metabolism of fucosylated oligosaccharides identified a Bifidobacterium bifidum α1-2- l -fucosidase that exhibited an atypical inverting mechanism (glycoside hydrolase [GH] family 95), termed AfcA ( 10 , 22 ). (asm.org)
- The crystal structure of an alpha-(1-3,4)-fucosidase from Bifidobacterium longum subsp. (expasy.org)
Activity11
- It was in a recent study by Endreffy, Bjørklund and collaborators (2017) found an association between the activity of α-L-fucosidase-1 (FUCA-1) and chronic autoimmune disorders in children. (wikipedia.org)
- One Unit of α-L-fucosidase activity is defined as the amount of enzyme required to release one µmole of p -nitrophenol ( p NP) per minute from p -nitrophenyl-α-L-fucopyranoside (1 mM) in sodium acetate buffer (100 mM) at pH 4.0 at the temperatures indicated. (megazyme.com)
- The pH 7 activity optimum of alpha-L-fucosidase and its subunit composition (one or two closely spaced immunoreactive protein bands of about 53+/-2 kDa) did not appear to change during transit through the epididymis. (semanticscholar.org)
- Fucosidosis is an autosomal recessive lysosomal storage disorder caused by reduced or absent alpha-L-fucosidase enzyme activity. (testcatalog.org)
- Transglycosylating activity of the alpha-L-fucosidase was demonstrated in reactions with such acceptors as alcohols, N-acetylglucosamine and N-acetylgalactosamine while no transglycosylation products were observed in the reaction with p-nitrophenyl alpha-L-fucopyranoside. (mysciencework.com)
- Activity-based screening of a genomic library was used to isolate the gene encoding a novel α-L-fucosidase. (biomedcentral.com)
- In the present study, plasma activity of α-L-fucosidase-1 (FUCA-1) and its potential association with chronic inflammatory pathologies was evaluated in hospitalized individuals, both pediatric and adult ones. (elsevier.com)
- Homozygous knockout mice completely lack α-L-fucosidase activity in all tested organs leading to highly elevated amounts of the core-fucosylated glycoasparagine Fuc(α1,6)-GlcNAc(β1-N)-Asn and, to a lesser extent, other fucosylated glycoasparagines, which all were also partially excreted in urine. (biologists.org)
- Single strand conformation polymorphism (SSCP) analysis followed by direct sequencing of amplified exons and flanking regions identified putative disease causing mutations in six of the patients, who had severe forms of the disease and very low residual alpha-L-fucosidase activity and protein. (bmj.com)
- The eighth patient, who had a partial deficiency of alpha-L-fucosidase in her fibroblasts and leucocytes at a young age but normal alpha-L-fucosidase activity and protein at a later age, was homozygous for the common Q281R polymorphism in exon 5. (bmj.com)
- FUCA1.1 has several biochemical functions, for example, alpha-L-fucosidase activity. (creativebiomart.net)
Enzymes6
- Alpha-fucosidase may refer to one of two enzymes: Fucosidase Alpha-L-fucosidase This set index page lists enzyme articles associated with the same name. (wikipedia.org)
- α- l -Fucosidases are enzymes involved in metabolism of α- l -fucosylated molecules, compounds with a fundamental role in different life essential processes including immune response, fertilization and development, but also in some serious pathological events. (biomedcentral.com)
- Fucosylated glycoconjugates are involved in numerous biological events, making alpha-l-fucosidases, the enzymes responsible for their processing, critically important. (embl-heidelberg.de)
- Sensitive carbohydrate chemistry techniques require specific enzymes such as fucosidase, sialidase, biosidase, and other hydrolytic enzymes with activities specific for certain linkages. (cellartis.com)
- Precise, pure enzymes from Takara Bio for carbohydrate chemistry analyses include alpha-1,2 fucosidase, alpha-1,3/1,4 fucosidase, alpha 2,3-sialidase, Lacto-N-biosidase, and glycan hydrolases useful for sphingolipid analysis such as recombinant endoglycoceramidase II. (cellartis.com)
- Despite this, few studies have focussed on xyloglucan saccharification, which requires a consortium of enzymes including endo-xyloglucanases, alpha-xylosidases, betagalactosidases and alpha-l-fucosidases, among others. (proteopedia.org)
Characterization1
- PDF] Alteration of the isoform composition of plasma-membrane-associated rat sperm alpha-L-fucosidase during late epididymal maturation: comparative characterization of the acidic and neutral isoforms. (semanticscholar.org)
Degradation of fucosylated2
- Alpha-fucosidase involved in degradation of fucosylated xyloglucans. (mybiosource.com)
- Fucosidosis is a rare lysosomal storage disorder caused by the inherited deficiency of the lysosomal hydrolase α-L-fucosidase, which leads to an impaired degradation of fucosylated glycoconjugates. (biologists.org)
Purification2
- Purification and properties of alpha-L-fucosidase from Chamelea gallina L". European Journal of Biochemistry / FEBS. (wikipedia.org)
- The enzyme was expressed in E.coli and affinity chromatography was used for purification of His-tagged α-L-fucosidase. (biomedcentral.com)
Encodes1
- AXY8 encodes an alpha-fucosidase, underscoring the importance of apoplastic metabolism on the fine structure of Arabidopsis cell wall polysaccharides. (mpg.de)
Hydrolase3
- In CAZy, alpha-L-fucosidases are found in glycoside hydrolase family 29 and glycoside hydrolase family 95. (wikipedia.org)
- Hot spots for protein partnership at the surface of cholinesterases and related alpha/beta hydrolase fold proteins or domains - a structural perspective. (univ-mrs.fr)
- Relationships of human alpha/beta hydrolase fold proteins and other organophosphate-interacting proteins. (univ-mrs.fr)
Carbohydrate1
- In this study we show that mAbs specific for the carbohydrate Ag Lewis x (CD15, Gal-beta 1-4 GlcNAc alpha 1-3Fuc) inhibit multimeric rCD2 binding to CD2L. (jimmunol.org)
Abstract2
- abstract = "(3R, 4R)-4-hydroxy-3-hydroxymethylpyrrolidine (6) was prepared from D-xylose and found to be a weak inhibitor of alpha-D-, beta-D-glucosidase and alpha-L-fucosidase. (dtu.dk)
- abstract = "Human serum α-l-fucosidase has been purified 241 200-fold with 35% yield by an affinity Chromatographie procedure utilizing agarose-ε{lunate}-aminocaproyl-fucosamine. (elsevier.com)
Specificity2
- Substrate specificity of alpha 1,2-L-fucosidase (Cat. (takarabio.com)
- Substrate specificity for alpha 1,3/1,4 fucosidase (Cat. (cellartis.com)
Longum1
- The afcA and afcB genes were introduced into the B. longum 105-A strain, which has no intrinsic - l -fucosidase. (scialert.net)
Isoenzyme2
- Using a genomic library of Paenibacillus thiaminolyticus , constructed in E.coli DH5α cells, nucleotide sequence of a new α- l -fucosidase isoenzyme was determined and submitted to the EMBL database (HE654122). (biomedcentral.com)
- Differences in the isoenzyme pattern of alpha-L-fucosidase were found in chorionic villi and maternal decidua. (ac.ke)
Homo1
- High purity α-(1-2,3,4,6)-L-Fucosidase ( Homo sapiens ) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. (megazyme.com)
Substrate2
- Chromogenic substrate for α-L-Fucosidase. (gbiosciences.com)
- Using the p-nitrophenyl substrate, the purified serum α-l-fucosidase had an apparent Michaelis constant of 0.52 mM and a broad pH optimum centered around pH 4.8 with a second, minor optimum at pH 6.1. (elsevier.com)
Glycosyl1
- Identification of the catalytic nucleophile of the family 29 alpha-L-fucosidase from Thermotoga maritima through trapping of a covalent glycosyl-enzyme intermediate and mutagenesis. (semanticscholar.org)
Thermus1
- An α-L-fucosidase from Thermus sp. (semanticscholar.org)
AfcA1
- 2) linkage of 2'-fucosyllactose, and a gene encoding 1,2-alpha-l-fucosidase (AfcA) was isolated. (semanticscholar.org)
Reactions1
- α- l -Fucosidases catalyzing transglycosylation reactions might be interesting in synthesis of α- l -fucosylated molecules with potential application especially in pharmaceutical industry, as these molecules are involved in many life-important or life-threatening processes in eukaryotic organisms. (biomedcentral.com)
Glycan structures1
- This enzyme is also called almond emulsin fucosidase I . This enzyme participates in the degradation of glycan structures. (leparisien.fr)
Cleavage1
- This project aimed to discover and analyze the molecular mechanism of synthesis of two particular fucosylated oligosaccharide products in a mutant enzyme, Thermatoga maratima Alpha-L-Fucosidase D224G, whose wild type performs the opposite reaction (cleavage of fucosyl glycosidic bonds). (umich.edu)