alpha Karyopherins
Nucleocytoplasmic transport molecules that bind to the NUCLEAR LOCALIZATION SIGNALS of cytoplasmic molecules destined to be imported into the CELL NUCLEUS. Once attached to their cargo they bind to BETA KARYOPHERINS and are transported through the NUCLEAR PORE COMPLEX. Inside the CELL NUCLEUS alpha karyopherins dissociate from beta karypherins and their cargo. They then form a complex with CELLULAR APOPTOSIS SUSCEPTIBILITY PROTEIN and RAN GTP-BINDING PROTEIN which is exported to the CYTOPLASM.
Karyopherins
A family of proteins involved in NUCLEOCYTOPLASMIC TRANSPORT. Karyopherins are heteromeric molecules composed two major types of components, ALPHA KARYOPHERINS and BETA KARYOPHERINS, that function together to transport molecules through the NUCLEAR PORE COMPLEX. Several other proteins such as RAN GTP BINDING PROTEIN and CELLULAR APOPTOSIS SUSCEPTIBILITY PROTEIN bind to karyopherins and participate in the transport process.
beta Karyopherins
Nucleocytoplasmic transport molecules that bind to ALPHA KARYOPHERINS in the CYTOSOL and are involved in transport of molecules through the NUCLEAR PORE COMPLEX. Once inside the CELL NUCLEUS beta karyopherins interact with RAN GTP-BINDING PROTEIN and dissociate from alpha karyopherins. Beta karyopherins bound to RAN GTP-BINDING PROTEIN are then re-transported to the cytoplasm where hydrolysis of the GTP of RAN GTP-BINDING PROTEIN causes release of karyopherin beta.
Nuclear Pore
Active Transport, Cell Nucleus
ran GTP-Binding Protein
Nuclear Pore Complex Proteins
Nuclear Localization Signals
Cell Nucleus
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Saccharomyces cerevisiae Proteins
Nuclear Proteins
Saccharomyces cerevisiae
Receptors, Cytoplasmic and Nuclear
Intracellular receptors that can be found in the cytoplasm or in the nucleus. They bind to extracellular signaling molecules that migrate through or are transported across the CELL MEMBRANE. Many members of this class of receptors occur in the cytoplasm and are transported to the CELL NUCLEUS upon ligand-binding where they signal via DNA-binding and transcription regulation. Also included in this category are receptors found on INTRACELLULAR MEMBRANES that act via mechanisms similar to CELL SURFACE RECEPTORS.
Protein Binding
alpha 1-Antitrypsin
Receptors, Adrenergic, alpha
One of the two major pharmacological subdivisions of adrenergic receptors that were originally defined by the relative potencies of various adrenergic compounds. The alpha receptors were initially described as excitatory receptors that post-junctionally stimulate SMOOTH MUSCLE contraction. However, further analysis has revealed a more complex picture involving several alpha receptor subtypes and their involvement in feedback regulation.
Hypoxia-Inducible Factor 1, alpha Subunit
alpha7 Nicotinic Acetylcholine Receptor
Integrin alpha3beta1
Cell surface receptor for LAMININ, epiligrin, FIBRONECTINS, entactin, and COLLAGEN. Integrin alpha3beta1 is the major integrin present in EPITHELIAL CELLS, where it plays a role in the assembly of BASEMENT MEMBRANE as well as in cell migration, and may regulate the functions of other integrins. Two alternatively spliced isoforms of the alpha subunit (INTEGRIN ALPHA3), are differentially expressed in different cell types.
Integrin alpha4
Integrin alpha6
An integrin alpha subunit that primarily associates with INTEGRIN BETA1 or INTEGRIN BETA4 to form laminin-binding heterodimers. Integrin alpha6 has two alternatively spliced isoforms: integrin alpha6A and integrin alpha6B, which differ in their cytoplasmic domains and are regulated in a tissue-specific and developmental stage-specific manner.
Integrin alpha5beta1
Integrin alpha4beta1
Integrin alpha4beta1 is a FIBRONECTIN and VCAM-1 receptor present on LYMPHOCYTES; MONOCYTES; EOSINOPHILS; NK CELLS and thymocytes. It is involved in both cell-cell and cell- EXTRACELLULAR MATRIX adhesion and plays a role in INFLAMMATION, hematopoietic cell homing and immune function, and has been implicated in skeletal MYOGENESIS; NEURAL CREST migration and proliferation, lymphocyte maturation and morphogenesis of the PLACENTA and HEART.
Interleukin-1alpha
Integrin alpha2beta1
An integrin found on fibroblasts, platelets, endothelial and epithelial cells, and lymphocytes where it functions as a receptor for COLLAGEN and LAMININ. Although originally referred to as the collagen receptor, it is one of several receptors for collagen. Ligand binding to integrin alpha2beta1 triggers a cascade of intracellular signaling, including activation of p38 MAP kinase.
Receptors, Adrenergic, alpha-1
Integrin alpha5
Integrin alpha1beta1
Integrin alpha1beta1 functions as a receptor for LAMININ and COLLAGEN. It is widely expressed during development, but in the adult is the predominant laminin receptor (RECEPTORS, LAMININ) in mature SMOOTH MUSCLE CELLS, where it is important for maintenance of the differentiated phenotype of these cells. Integrin alpha1beta1 is also found in LYMPHOCYTES and microvascular endothelial cells, and may play a role in angiogenesis. In SCHWANN CELLS and neural crest cells, it is involved in cell migration. Integrin alpha1beta1 is also known as VLA-1 and CD49a-CD29.
Nucleoplasmins
Databases, Protein
Internet
User-Computer Interface
Authorship
Knowledge Bases
Dictionaries as Topic
GTP-Binding Proteins
Regulatory proteins that act as molecular switches. They control a wide range of biological processes including: receptor signaling, intracellular signal transduction pathways, and protein synthesis. Their activity is regulated by factors that control their ability to bind to and hydrolyze GTP to GDP. EC 3.6.1.-.
Phosphorylation-dependent binding of hepatitis B virus core particles to the nuclear pore complex. (1/392)
Although many viruses replicate in the nucleus, little is known about the processes involved in the nuclear import of viral genomes. We show here that in vitro generated core particles of human hepatitis B virus bind to nuclear pore complexes (NPCs) in digitonin-permeabilized mammalian cells. This only occurred if the cores contained phosphorylated core proteins. Binding was inhibited by wheat germ agglutinin, by antinuclear pore complex antibodies, and by peptides corresponding either to classical nuclear localization signals (NLS) or to COOH-terminal sequences of the core protein. Binding was dependent on the nuclear transport factors importins (karyopherins) alpha and beta. The results suggested that phosphorylation induces exposure of NLS in the COOH-terminal portion of the core protein that allows core binding to the NPCs by the importin- (karyopherin-) mediated pathway. Thus, phosphorylation of the core protein emerged as an important step in the viral replication cycle necessary for transport of the viral genome to the nucleus. (+info)Deciphering the nuclear import pathway for the cytoskeletal red cell protein 4.1R. (2/392)
The erythroid membrane cytoskeletal protein 4.1 is the prototypical member of a genetically and topologically complex family that is generated by combinatorial alternative splicing pathways and is localized at diverse intracellular sites including the nucleus. To explore the molecular determinants for nuclear localization, we transfected COS-7 cells with epitope-tagged versions of natural red cell protein 4.1 (4.1R) isoforms as well as mutagenized and truncated derivatives. Two distant topological sorting signals were required for efficient nuclear import of the 4.1R80 isoform: a basic peptide, KKKRER, encoded by alternative exon 16 and acting as a weak core nuclear localization signal (4.1R NLS), and an acidic peptide, EED, encoded by alternative exon 5. 4.1R80 isoforms lacking either of these two exons showed decreased nuclear import. Fusion of various 4.1R80 constructs to the cytoplasmic reporter protein pyruvate kinase confirmed a requirement for both motifs for full NLS function. 4.1R80 was efficiently imported in the nuclei of digitonin-permeabilized COS-7 cells in the presence of recombinant Rch1 (human importin alpha2), importin beta, and GTPase Ran. Quantitative analysis of protein-protein interactions using a resonant mirror detection technique showed that 4.1R80 bound to Rch1 in vitro with high affinity (KD = 30 nM). The affinity decreased at least 7- and 20-fold, respectively, if the EED motif in exon 5 or if 4.1R NLS in exon 16 was lacking or mutated, confirming that both motifs were required for efficient importin-mediated nuclear import of 4.1R80. (+info)Nuclear import of plasmid DNA in digitonin-permeabilized cells requires both cytoplasmic factors and specific DNA sequences. (3/392)
Although much is known about the mechanisms of signal-mediated protein and RNA nuclear import and export, little is understood concerning the nuclear import of plasmid DNA. Plasmids between 4.2 and 14.4 kilobases were specifically labeled using a fluorescein-conjugated peptide nucleic acid clamp. The resulting substrates were capable of gene expression and nuclear localization in microinjected cells in the absence of cell division. To elucidate the requirements for plasmid nuclear import, a digitonin-permeabilized cell system was adapted to follow the nuclear localization of plasmids. Nuclear import of labeled plasmid was time- and energy-dependent, was inhibited by the lectin wheat germ agglutinin, and showed an absolute requirement for cytoplasmic extract. Addition of nuclear extract alone did not support plasmid nuclear import but in combination with cytoplasm stimulated plasmid nuclear localization. Whereas addition of purified importin alpha, importin beta, and RAN was sufficient to support protein nuclear import, plasmid nuclear import also required the addition of nuclear extract. Finally, nuclear import of plasmid DNA was sequence-specific, requiring a region of the SV40 early promoter and enhancer. Taken together, these results confirm and extend our findings in microinjected cells and support a protein-mediated mechanism for plasmid nuclear import. (+info)Nuclear import of RPA in Xenopus egg extracts requires a novel protein XRIPalpha but not importin alpha. (4/392)
Replication protein A (RPA) is a eukaryotic single-stranded (ss) DNA-binding protein that is essential for general DNA metabolism. RPA consists of three subunits (70, 33 and 14 kDa). We have identified by two-hybrid screening a novel Xenopus protein called XRIPalpha that interacts with the ssDNA-binding domain of the largest subunit of RPA. XRIPalpha homologues are found in human and in Drosophila but not in yeast. XRIPalpha is complexed with RPA in Xenopus egg extracts together with another 90 kDa protein that was identified as importin beta. We have demonstrated that XRIPalpha, but not importin alpha, is required for nuclear import of RPA. Immunodepletion of XRIPalpha from the egg extracts blocks nuclear import of RPA but not that of nucleoplasmin, a classical import substrate. RPA import can be restored by addition of recombinant XRIPalpha. Conversely, depletion of importin alpha blocks import of nucleoplasmin but not that of RPA. GST-XRIPalpha pull-down assay shows that XRIPalpha interacts directly with recombinant importin beta as well as with RPA in vitro. Finally, RPA import can be reconstituted from the recombinant proteins. We propose that XRIPalpha plays the role of importin alpha in the RPA import scheme: XRIPalpha serves as an adaptor to link RPA to importin beta. (+info)The direction of transport through the nuclear pore can be inverted. (5/392)
Transport of macromolecules across the nuclear envelope is an active process that depends on soluble factors including the GTPase Ran. Ran-GTP is predominantly located in the nucleus and has been shown to regulate cargo binding and release of import and export receptors in their respective target compartments. Recently, it was shown that transport of receptor-cargo complexes across the nuclear pore complex (NPC) does not depend on GTP-hydrolysis by Ran; however, the mechanism of translocation is still poorly understood. Here, we show that the direction of transport through the NPC can be inverted in the presence of high concentrations of cytoplasmic Ran-GTP. Under these conditions, two different classes of export cargoes are transported into the nucleus in the absence of GTP hydrolysis. The inverted transport is very rapid and can be blocked by known inhibitors of nuclear protein export. These results suggest that the NPC functions as a facilitated transport channel, allowing the selective translocation of receptor-cargo complexes. We conclude that the directionality of nucleocytoplasmic transport is determined mainly by the compartmentalized distribution of Ran-GTP. (+info)Cellular uptake and nuclear delivery of recombinant adenovirus penton base. (6/392)
An Ad2 capsid component, the penton base, expressed as recombinant protein, was found to be capable of affecting the entire entry pathway of adenovirion in HeLa cells, i.e., cell attachment, endocytosis, vesicular escape, intracytoplasmic movement, and translocation through the nuclear pore complex. Data with pentamerization-defective mutants suggested that none of these successive steps depended upon penton base pentamer status, indicating that the peptide domains responsible for these functions were carried by the monomer. Observations performed with wild-type (WT) and an integrin-binding-site double-mutant (K288E340) suggested that the penton base could enter the cell via an alternative, RGD- and LDV-independent, pathway. Of three mutants that were found to be defective in nuclear addressing in insect cells, only one, W165H, was also altered in nuclear transport in HeLa cells. The other two, W119H and RRR547EQQ, showed a WT pattern of nuclear localization in HeLa cells, suggesting that the region including tryptophan-119 and the basic signal at position 547 did not act as a nuclear localization signal in the human cell context. The integrity of cellular structures and the cytoskeleton seemed to be required for the vectorial movement and nuclear import of WT penton base, as suggested by experiments using permeabilized HeLa cells, isolated nuclear membranes, and cytoskeleton-targeted drugs. (+info)The yeast nucleoporin Nup2p is involved in nuclear export of importin alpha/Srp1p. (7/392)
The importin alpha.beta heterodimer mediates nuclear import of proteins containing classical nuclear localization signals. After carrying its cargo into the nucleus, the importin dimer dissociates, and Srp1p (the yeast importin alpha subunit) is recycled to the cytoplasm in a complex with Cse1p and RanGTP. Nup2p is a yeast FXFG nucleoporin that contains a Ran-binding domain. We find that export of Srp1p from the nucleus is impaired in Deltanup2 mutants. Also, Srp1p fusion proteins accumulate at the nuclear rim in wild-type cells but accumulate in the nuclear interior in Deltanup2 cells. A deletion of NUP2 shows genetic interactions with mutants in SRP1 and PRP20, which encodes the Ran nucleotide exchange factor. Srp1p binds directly to an N-terminal domain of Nup2p. This region of Nup2p is sufficient to allow accumulation of an Srp1p fusion protein at the nuclear rim, but the C-terminal Ran-binding domain of Nup2p is required for efficient Srp1p export. Formation of the Srp1p.Cse1p. RanGTP export complex releases Srp1p from its binding site in Nup2p. We propose that Nup2p may act as a scaffold that facilitates formation of the Srp1p export complex. (+info)A bipartite nuclear localization signal is required for p53 nuclear import regulated by a carboxyl-terminal domain. (8/392)
Abnormal p53 cellular localization has been considered to be one of the mechanisms that could inactivate p53 function. To understand the regulation of p53 cellular trafficking, we have previously identified two p53 domains involved in its localization. A basic domain, Lys(305)-Arg(306), is required for p53 nuclear import, and a carboxyl-terminal domain, namely the cytoplasmic sequestration domain (CSD) from residues 326-355, could block the nuclear import of Lys(305) or Arg(306) mutated p53. To characterize further the function of these two domains, we demonstrate in this report that the previously described major nuclear localization signal works together with Lys(305)-Arg(306) to form a bipartite and functional nuclear localization sequence (NLS) for p53 nuclear import. The CSD could block the binding of p53 to the NLS receptor, importin alpha, and reduce the efficiency of p53 nuclear import in MCF-7, H1299, and Saos-2 cells. The blocking effect of the CSD is not due to the enhancement of nuclear export or oligomerization of the p53. These results indicate that the CSD can regulate p53 nuclear import by controlling access of the NLS to importin alpha binding. (+info)
Structural characterisation of the nuclear import receptor importin alpha in complex with the bipartite NLS of Prp20 - UQ eSpace
KPNA6 karyopherin subunit alpha 6 [Homo sapiens (human)] - Gene - NCBI
TCDB » SEARCH
Catalyst 3750 Switch Software Configuration Guide, 12.2(52)SE - Configuring HSRP [Cisco Catalyst 3750 Series Switches] - Cisco
KPNA4 / Importin alpha-4 antibody | acris-antibodies.com
Yeast karyopherin Kap95 is required for cell cycle progression at Start | BMC Molecular and Cell Biology | Full Text
NP - Nucleoprotein - Influenza A virus (strain A/Wilson-Smith/1933 H1N1) - NP gene & protein
CSE1L chromosome segregation 1 like [Homo sapiens (human)] - Gene - NCBI
Importin alpha 5/KPNA1/SRP1 Research Products: Novus Biologicals
KPNA2 Antibody | scrazzl.com - Simply Connecting Science
WikiGenes - KPNA4 - karyopherin alpha 4 (importin alpha 3)
PLOS ONE: Identification of Karyopherin α1 and α7 Interacting Proteins in Porcine Tissue
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Karyopherin (importin) beta 3 RNAi: Novus Biologicals
OPUS Würzburg | Search
Nuclear import substrates compete for a limited number of binding sites : evidence for different classes of yeast nuclear...
anti-KPNA3 antibody, C-term | GeneTex
Addgene: pCMVTNT-T7-KPNA2
Protein-analysis] What do you use for MSA and Design?
SSR3 - PCR Primer Pair - SYBR | PrimePCR | Bio-Rad
KPNA6抗体(ab105350)| Abcam中国
KPNA2抗体- N-terminal|Abcam中国|Anti-KPNA2抗体- N-terminal
Acetic Acid Market Growth Rate 2019 | Rising Trends, Demand Status and Growth Potential in Chemicals & Materials Sector...
Karyopherin alpha 5 | definition of karyopherin alpha 5 by Medical dictionary
Significant Proportions of Nuclear Transport Proteins with Reduced Intracellular Mobilities Resolved by Fluorescence...
HSRP1 | definition of hSRP1 by Medical dictionary
OpenEmory | Search Results
Six classes of nuclear localization signals specific to different binding grooves of importinα<...
Importin - Wikipedia
Lecture 6 10 - Lecture 6 Nuclear Import/Export Nuclear Import Receptors Importins Helps cargo get into nucleus does this by...
Bipartite Nuclear Localization Signal Controls Nuclear Import and DNA-Binding Activity of IFN Regulatory Factor 3 | The Journal...
Mutagenetix > Incidental...
KPNA6 - Importin subunit alpha-7 - Homo sapiens (Human) - KPNA6 gene & protein
Nuclear localization of the DNA repair scaffold XRCC1: Uncovering the functional role of a bipartite NLS (Journal Article) |...
Exportin-2
Nukleärer Import von 19S regulatorischen Komplexen in der Hefe Saccharomyces cerevisiae
karyopherin beta 3 CRISPR Plasmids | SCBT - Santa Cruz Biotechnology
KPNB1 (karyopherin (importin) beta 1)
Structure Cluster
- 1EE5: YEAST KARYOPHERIN (IMPORTIN) ALPHA IN A COMPLEX WITH A NUCLEOPLASMIN NLS PEPTIDE 3D...
EuRBPDB
Quality Improvement Plan
Importin and nucleoplasmin complex - Stock Image C025/1566 - Science Photo Library
Importin
Karyopherin (zebrafish) | Protein Target - PubChem
QIP 2014 Accepted Papers
RCSB PDB
- 4BA3: mImp alphadIBB A89NLS Macromolecule Annotations Page
Recombinant Human KPNA2 protein (ab123205) | Abcam
beta Karyopherins
- Importin-beta
Summary Report | CureHunter
Yeast N1e3p/Nup170p is required for normal stoichiometry of FG nucleoporins within the nuclear pore complex. | Molecular and...
Peroxisomal long-chain fatty acid import protein 1 (P34230) - Yeast Metabolome Database
Gene Ontology Classifications
How mRNAs traffic to the endoplasmic reticulum and controls protein import | Department of molecular genetics
QIP, A Component of the Vegetative RNA Silencing Pathway, is Essential for Meiosis and Suppresses Meiotic Silencing in...
admin, Author at Hand Therapy
Long-Distance Distress Signal from Periphery of Injured Nerve Cells Begins with Locally Made Protein | CoAS | Drexel University
Info Req. on Source of Nuclear Localization Signal for lac-Z gene.
NLS LENGTH SEMANTICS = CHAR import is too slow
Tokotna Import Information Error Reports
TMCO6
The SRP1 domain is Karyopherin(importin) alpha. This is involved in the exchange of molecules from the nucleus and the ... The SRP1 domain encodes alpha-Karyopherin (importin) and is known for intracellular trafficking and secretion on the membrane. ... The exchange involves the active transport by a carrier protein called karyopherins. A di-leucine motif is also abundant in ...
KPNA3
"Entrez Gene: KPNA3 karyopherin alpha 3 (importin alpha 4)". Köhler, M; Speck C; Christiansen M; Bischoff F R; Prehn S; Haller H ... The similarities among these proteins suggests that karyopherin alpha-3 may be involved in the nuclear transport system. KPNA3 ... "Isolation and mapping of karyopherin alpha 3 (KPNA3), a human gene that is highly homologous to genes encoding Xenopus importin ... Importin subunit alpha-3 is a protein that in humans is encoded by the KPNA3 gene. The transport of molecules between the ...
KPNA6
"Entrez Gene: KPNA6 karyopherin alpha 6 (importin alpha 7)". Bukrinsky MI, Haffar OK (2004). "HIV-1 nuclear import: in search of ... a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in ... Importin subunit alpha-7 is a protein that in humans is encoded by the KPNA6 gene. Nucleocytoplasmic transport, a signal- and ... Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. The protein encoded by ...
KPNA4
karyopherin subunit alpha-4, also known as importin alpha-3, is a protein that in humans is encoded by the KPNA4 gene. The ... "Entrez Gene: KPNA4 karyopherin alpha 4 (importin alpha 3)". Liu L, McBride KM, Reich NC (Jun 2005). "STAT3 nuclear import is ... "Cloning of two novel human importin-alpha subunits and analysis of the expression pattern of the importin-alpha protein family ... Seki T, Tada S, Katada T, Enomoto T (May 1997). "Cloning of a cDNA encoding a novel importin-alpha homologue, Qip1: ...
KPNA5
"Entrez Gene: KPNA5 karyopherin alpha 5 (importin alpha 6)". Bukrinsky MI, Haffar OK (2004). "HIV-1 nuclear import: in search of ... "Cloning of two novel human importin-alpha subunits and analysis of the expression pattern of the importin-alpha protein family ... Importin subunit alpha-6 is a protein that in humans is encoded by the KPNA5 gene. The transport of molecules between the ... 1996). "Role of the karyopherin pathway in human immunodeficiency virus type 1 nuclear import". J. Virol. 70 (2): 1027-32. doi: ...
ARL4A
... has been shown to interact with Karyopherin alpha 2. GRCh38: Ensembl release 89: ENSG00000122644 - Ensembl, May 2017 ...
PLAG1
Braem CV, Kas K, Meyen E, Debiec-Rychter M, Van De Ven WJ, Voz ML (2002). "Identification of a karyopherin alpha 2 recognition ... PLAG1 has been shown to interact with Karyopherin alpha 2. GRCh38: Ensembl release 89: ENSG00000181690 - Ensembl, May 2017 ... "Identification of a karyopherin alpha 2 recognition site in PLAG1, which functions as a nuclear localization signal". J. Biol. ...
RECQL
... has been shown to interact with KPNA4 and Karyopherin alpha 2. GRCh38: Ensembl release 89: ENSG00000004700 - Ensembl, May ... Seki T, Tada S, Katada T, Enomoto T (1997). "Cloning of a cDNA encoding a novel importin-alpha homologue, Qip1: discrimination ... "Cloning of a cDNA encoding a novel importin-alpha homologue, Qip1: discrimination of Qip1 and Rch1 from hSrp1 by their ability ...
Nucleoporin 153
Moroianu J, Blobel G, Radu A (1997). "RanGTP-mediated nuclear export of karyopherin alpha involves its interaction with the ... It also acts as the docking site of an importing karyopherin. On the cytoplasmic side of the NPC, Nup358 fulfills an analogous ... Yaseen NR, Blobel G (1997). "Cloning and characterization of human karyopherin beta3". Proc. Natl. Acad. Sci. U.S.A. 94 (9): ... Brownawell AM, Macara IG (2002). "Exportin-5, a novel karyopherin, mediates nuclear export of double-stranded RNA binding ...
UBR5
... has been shown to interact with: CIB1, Karyopherin alpha 1, MAPK1, and TOPBP1. GRCh38: Ensembl release 89: ENSG00000104517 ...
KPNB1
... has been shown to interact with: KPNA3, Karyopherin alpha 1, Karyopherin alpha 2, Mothers against decapentaplegic homolog ... Each of these subunits are part of the karyopherin family of proteins. Importin alpha binds the NLS-containing cargo in the ... Moroianu J, Blobel G, Radu A (September 1997). "RanGTP-mediated nuclear export of karyopherin alpha involves its interaction ... Ran-GTP dissociates the karyopherin alphabeta heterodimer by displacing alpha from an overlapping binding site on beta". Proc. ...
NUP98
Moroianu J, Blobel G, Radu A (September 1997). "RanGTP-mediated nuclear export of karyopherin alpha involves its interaction ... Bonifaci N, Moroianu J, Radu A, Blobel G (May 1997). "Karyopherin beta2 mediates nuclear import of a mRNA binding protein". ... Yaseen NR, Blobel G (1997). "Cloning and characterization of human karyopherin beta3". Proc. Natl. Acad. Sci. U.S.A. 94 (9): ... Bonifaci N, Moroianu J, Radu A, Blobel G (1997). "Karyopherin beta2 mediates nuclear import of a mRNA binding protein". Proc. ...
Nuclear localization sequence
"Crystallographic analysis of the recognition of a nuclear localisation signal by the nuclear import factor karyopherin alpha". ... A protein translated with an NLS will bind strongly to importin (aka karyopherin), and, together, the complex will move through ... analysis of the specific yet versatile recognition of distinct nuclear localisation signals by karyopherin alpha". Structure. 8 ... also known as transportin or karyopherin β2), which then translocates the cargo protein into the nucleus. The structural basis ...
Colin Dingwall
"Crystallographic analysis of the recognition of a nuclear localisation signal by the nuclear import factor karyopherin alpha". ... The broad outline of this model was confirmed ten years later by the crystallographic analysis of importin alpha complexed with ...
Importin
"Crystallographic analysis of the recognition of a nuclear localization signal by the nuclear import factor karyopherin alpha". ... Importin: IPO4, IPO5, IPO7, IPO8, IPO9, IPO11, IPO13 Karyopherin-α: KPNA1, KPNA2, KPNA3, KPNA4, KPNA5, KPNA6 Karyopherin-β: ... Importin is a type of karyopherin that transports protein molecules from the cell's cytoplasm to the nucleus. It does so by ... Each one of these repeats contains two antiparallel alpha helices linked by a turn, which stack together to form the overall ...
ITK (gene)
"Nuclear localization of the tyrosine kinase Itk and interaction of its SH3 domain with karyopherin alpha (Rch1alpha)". Int. ...
KPNA6 - ويكيبيديا
"Entrez Gene: KPNA6 karyopherin alpha 6 (importin alpha 7)". مؤرشف من الأصل في 05 ديسمبر 2010.. الوسيط ,CitationClass=. تم ... KPNA6 (Karyopherin subunit alpha 6) هوَ بروتين يُشَفر بواسطة جين KPNA6 في الإنسان.[1][2] ... KPNA6, IPOA7, KPNA7, karyopherin subunit alpha 6. معرفات خارجية. الوراثة المندلية البشرية عبر الإنترنت 610563 MGI: MGI:1100836 ... Chook YM, Blobel G (2002). "Karyopherins and nuclear import". Curr. Opin. Struct. Biol. 11 (6): 703-15. doi:10.1016/S0959-440X( ...
Importin α
Importin alpha, or karyopherin alpha refers to a class of adaptor proteins that are involved in the import of proteins into the ... Moroianu, J; Blobel, G; Radu, A (25 June 1996). "The binding site of karyopherin alpha for karyopherin beta overlaps with a ... "Previously identified protein of uncertain function is karyopherin alpha and together with karyopherin beta docks import ... Wang, P.; Palese, P.; O'Neill, R. E. (1 March 1997). "The NPI-1/NPI-3 (karyopherin alpha) binding site on the influenza a virus ...
Ebola viral protein 24
August 2014). "Ebola virus VP24 targets a unique NLS binding site on karyopherin alpha 5 to selectively compete with nuclear ... KPNA proteins have 10 armadillo repeats each consisting of three alpha helices which determine their binding specificity, the ... Additionally, eVP24 inhibits interferon signaling by competitively binding to karyopherins which blocks phosphorylated STAT1 ... "The Ebola virus VP24 protein prevents hnRNP C1/C2 binding to karyopherin α1 and partially alters its nuclear import". The ...
List of MeSH codes (D12.776.157)
... karyopherins MeSH D12.776.157.530.750.500.100 - alpha karyopherins MeSH D12.776.157.530.750.500.249 - beta karyopherins MeSH ... gtp-binding protein alpha subunits, g12-g13 MeSH D12.776.157.325.332.100.200 - gtp-binding protein alpha subunits, gi-go MeSH ... gtp-binding protein alpha subunits, gq-g11 MeSH D12.776.157.325.332.100.400 - gtp-binding protein alpha subunits, gs MeSH ... alpha subunit MeSH D12.776.157.057.061.500.500 - stat1 transcription factor MeSH D12.776.157.057.061.500.750 - stat2 ...
IRF1
"Differential regulation of karyopherin alpha 2 expression by TGF-beta1 and IFN-gamma in normal human epidermal keratinocytes: ...
Transportin 1
"Mammalian karyopherin alpha 1 beta and alpha 2 beta heterodimers: alpha 1 or alpha 2 subunit binds nuclear localization signal ... The classical karyopherin receptor complex, such as the complex that uses Importin-β1 (encoded by gene KPNB1), is a heterodimer ... However, Transportin-1 can directly bind to the cargo proteins and may not need importin alpha subunit to do it. Transportin-1 ... Bonifaci N, Moroianu J, Radu A, Blobel G (Jun 1997). "Karyopherin beta2 mediates nuclear import of a mRNA binding protein". ...
IPO5
... a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in ... Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between ... May 2020). "X-ray Structure of the Human Karyopherin RanBP5, an Essential Factor for Influenza Polymerase Nuclear Trafficking ... Yaseen NR, Blobel G (April 1997). "Cloning and characterization of human karyopherin beta3". Proceedings of the National ...
Karyopherin
First, it is binding importin alpha - another type of karyopherin that binds the cargo protein in the cytoplasm-before the ... Most proteins require karyopherins to traverse the nuclear pore. Karyopherins can act as importins (i.e. helping proteins get ... Karyopherins at the US National Library of Medicine Medical Subject Headings (MeSH) Illustrations at berkeley.edu Karyopherin ... Karyopherins are proteins involved in transporting molecules between the cytoplasm and the nucleus of a eukaryotic cell. The ...
Karyopherin alpha 1
"Mammalian karyopherin alpha 1 beta and alpha 2 beta heterodimers: alpha 1 or alpha 2 subunit binds nuclear localization signal ... Importin subunit alpha-1 is a protein that in humans is encoded by the KPNA1 gene. Karyopherin alpha 1 has been shown to ... "Previously identified protein of uncertain function is karyopherin alpha and together with karyopherin beta docks import ... Percipalle, P; Clarkson W D; Kent H M; Rhodes D; Stewart M (Mar 1997). "Molecular interactions between the importin alpha/beta ...
Karyopherin alpha 2
"Mammalian karyopherin alpha 1 beta and alpha 2 beta heterodimers: alpha 1 or alpha 2 subunit binds nuclear localization signal ... "Entrez Gene: KPNA2 karyopherin alpha 2 (RAG cohort 1, importin alpha 1)". Lin CY, Huang PH, Liao WL, Cheng HJ, Huang CF, Kuo JC ... KPNA2 also may play a role in V(D)J recombination Karyopherin alpha 2 has been shown to interact with: ARL4A ITK, KPNB1, PLAG1 ... Braem CV, Kas K, Meyen E, Debiec-Rychter M, Van De Ven WJ, Voz ML (May 2002). "Identification of a karyopherin alpha 2 ...
Alpha solenoid
For example, the beta-karyopherin superfamily consists of alpha solenoid proteins formed from HEAT repeats; importin beta is a ... "alpha alpha superhelix". The CATH database uses the term "alpha horseshoe" for these proteins, and uses "alpha solenoid" for a ... An alpha solenoid (sometimes also known as an alpha horseshoe or as stacked pairs of alpha helices, abbreviated SPAH) is a ... Examples of alpha solenoid structures binding RNA and lipids have also been described. The term "alpha solenoid" has been used ...
List of MeSH codes (D12.776.543)
... karyopherins MeSH D12.776.543.585.750.500.100 - alpha karyopherins MeSH D12.776.543.585.750.500.249 - beta karyopherins MeSH ... gtp-binding protein alpha subunit, gi2 MeSH D12.776.543.325.100.300 - gtp-binding protein alpha subunits, gq-g11 MeSH D12.776. ... alpha-1 MeSH D12.776.543.750.720.300.300.300.300.200 - receptors, adrenergic, alpha-2 MeSH D12.776.543.750.720.300.300.300.340 ... alpha MeSH D12.776.543.750.070.300.300.300.700 - receptors, adrenergic, alpha-1 MeSH D12.776.543.750.070.300.300.300.725 - ...
Nucleoporin 62
Karyopherin beta2 (transportin), a riboprotein transporter also interacts with p62. P62 also interacts with Nup93, and when ... This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear ... Yaseen NR, Blobel G (1997). "Cloning and characterization of human karyopherin β3". Proc. Natl. Acad. Sci. U.S.A. 94 (9): 4451- ... Yaseen NR, Blobel G (1997). "Cloning and characterization of human karyopherin β3". Proc. Natl. Acad. Sci. U.S.A. 94 (9): 4451- ...
HEAT repeat
A HEAT repeat is a protein tandem repeat structural motif composed of two alpha helices linked by a short loop. HEAT repeats ... Representative examples of HEAT repeat proteins include importin β (also known as karyopherin β) family, regulatory subunits of ... "Structure of importin-beta bound to the IBB domain of importin-alpha". Nature. 399 (6733): 221-229. Bibcode:1999Natur.399..221C ... "Structure of the nuclear transport complex karyopherin-beta2-Ran x GppNHp". Nature. 399 (6733): 230-237. doi:10.1038/20375. ...
Cell nucleus
Most karyopherins interact directly with their cargo, although some use adaptor proteins.[11] Steroid hormones such as cortisol ... Like the components of other intermediate filaments, the lamin monomer contains an alpha-helical domain used by two monomers to ... Those karyopherins that mediate movement into the nucleus are also called importins, whereas those that mediate movement out of ... macromolecules such as RNA and proteins require association karyopherins called importins to enter the nucleus and exportins to ...
XPO1
"Npap60/Nup50 is a tri-stable switch that stimulates importin-alpha:beta-mediated nuclear protein import". Cell. 110 (3): 349-60 ... Karyopherin. References[edit]. *^ a b c GRCh38: Ensembl release 89: ENSG00000082898 - Ensembl, May 2017 ...
MicroRNA
Zuo Y, Qiang L, Farmer SR (March 2006). "Activation of CCAAT/enhancer-binding protein (C/EBP) alpha expression by C/EBP beta ... a member of the karyopherin family, recognizes a two-nucleotide overhang left by the RNase III enzyme Drosha at the 3' end of ... such as TNF alpha or GM-CSF. It has been demonstrated that given complete complementarity between the miRNA and target mRNA ... adipogenesis requires a peroxisome proliferator-activated receptor-gamma-associated repression of HDAC1 at the C/ebp alpha gene ...
RCC1
"The nucleoporin Nup98 is a site for GDP/GTP exchange on ran and termination of karyopherin beta 2-mediated nuclear import". The ... "Identification of nuclear-import and cell-cycle regulatory proteins that bind to prothymosin alpha". Biochemistry and Cell ... karyopherin alpha3/Qip". The Journal of Biological Chemistry. 275 (14): 10099-104. doi:10.1074/jbc.275.14.10099. PMID 10744690 ...
DUT (gene)
Though both isoforms contain the NLS, the sequence in DUT-M is sequestered away from cognate karyopherins. The isoelectric ... and identification of rat deoxyuridine triphosphatase as an inhibitor of peroxisome proliferator-activated receptor alpha". The ...
Cell nucleus
Those karyopherins that mediate movement into the nucleus are also called importins, whereas those that mediate movement out of ... Like the components of other intermediate filaments, the lamin monomer contains an alpha-helical domain used by two monomers to ... Most karyopherins interact directly with their cargo, although some use adaptor proteins. Steroid hormones such as cortisol and ... October 2007). "LPS-induced down-regulation of signal regulatory protein {alpha} contributes to innate immune activation in ...
Ran (protein)
Shamsher MK, Ploski J, Radu A (2002). "Karyopherin beta 2B participates in mRNA export from the nucleus". Proc. Natl. Acad. Sci ... Percipalle P, Clarkson WD, Kent HM, Rhodes D, Stewart M (1997). "Molecular interactions between the importin alpha/beta ... Ran is involved in the transport of proteins across the nuclear envelope by interacting with karyopherins and changing their ... Chook YM, Blobel G (1999). "Structure of the nuclear transport complex karyopherin-beta2-Ran x GppNHp". Nature. 399 (6733): 230 ...
NXF1
The Tap-C domain is made of four alpha helices packed against each other. The arrangement of helices 1, 2 and 3 is similar to ... Shamsher MK, Ploski J, Radu A (October 2002). "Karyopherin beta 2B participates in mRNA export from the nucleus". Proceedings ...
Transporter Classification Database
... including karyopherins 1.I.2 Plant plasmodesmata family 2.A.1 Major Facilitator superfamily (MFS), see also Lactose permease, ... Family 1.D.60 The Alpha,Gamma-Cyclic Peptide (AGCP) Family 1.D.61 The Anionophoric 2,6-Bis(Benzimidazol-2-yl)Pyridine (ABBP) ... Family 1.D.53 The alpha, gamma-Peptide Nanotube (a,gPepNT) Family 1.D.54 The potassium-selective Hexyl-Benzoureido-15-Crown-5- ... Ether Ion Channel (HBEC) Family 1.D.55 The Porphyrin-based Nanopore (PorNP) Family 1.D.56 The Alpha-Aminoisobutyrate (Aib) ...
Nuclear pore
... this family is further subdivided to the karyopherin-α and the karyopherin-β subfamilies. Other nuclear transport receptors ... of 34 distinct nucleoporin proteins.About half of the nucleoporins typically contain solenoid protein domains-either an alpha ... The largest family of nuclear transport receptors are karyopherins, which includes dozens of both importins and exportins; ...
List of A1 genes, proteins or receptors
... alpha 1 Interleukin 13 receptor, alpha 1 Karyopherin alpha 1 Laminin, alpha 1 Major histocompatibility complex, class II, DP ... alpha 1 collagen, type II, alpha 1 Collagen, type III, alpha 1 Collagen, type IV, alpha 1 Collagen, type V, alpha 1 Collagen, ... type VI, alpha 1 Collagen, type VII, alpha 1 Collagen, type VIII, alpha 1 Collagen, type IX, alpha 1 Collagen, type X, alpha 1 ... alpha 1 Collagen, type XIII, alpha 1 Collagen, type XIV, alpha 1 Collagen, type XV, alpha 1 Collagen, type XVI, alpha 1 ...
Karyopherin alpha 1 - Wikipedia
"Mammalian karyopherin alpha 1 beta and alpha 2 beta heterodimers: alpha 1 or alpha 2 subunit binds nuclear localization signal ... Importin subunit alpha-1 is a protein that in humans is encoded by the KPNA1 gene. Karyopherin alpha 1 has been shown to ... "Previously identified protein of uncertain function is karyopherin alpha and together with karyopherin beta docks import ... Percipalle, P; Clarkson W D; Kent H M; Rhodes D; Stewart M (Mar 1997). "Molecular interactions between the importin alpha/beta ...
Karyopherin alpha 2 - Wikipedia
"Mammalian karyopherin alpha 1 beta and alpha 2 beta heterodimers: alpha 1 or alpha 2 subunit binds nuclear localization signal ... "Entrez Gene: KPNA2 karyopherin alpha 2 (RAG cohort 1, importin alpha 1)". Lin CY, Huang PH, Liao WL, Cheng HJ, Huang CF, Kuo JC ... KPNA2 also may play a role in V(D)J recombination Karyopherin alpha 2 has been shown to interact with: ARL4A ITK, KPNB1, PLAG1 ... Braem CV, Kas K, Meyen E, Debiec-Rychter M, Van De Ven WJ, Voz ML (May 2002). "Identification of a karyopherin alpha 2 ...
KPNA6 karyopherin subunit alpha 6 [Homo sapiens (human)] - Gene - NCBI
importin subunit alpha-7. Names. importin alpha 7 subunit. importin-alpha-S2. karyopherin alpha 6 (importin alpha 7). ... KPNA6 karyopherin subunit alpha 6 [Homo sapiens] KPNA6 karyopherin subunit alpha 6 [Homo sapiens]. Gene ID:23633 ... HIV-1 Rev interacting protein, karyopherin alpha 6 (importin alpha 7) (KPNA6), is identified by the in-vitro binding ... karyopherin subunit alpha 6provided by HGNC. Primary source. HGNC:HGNC:6399 See related. Ensembl:ENSG00000025800 MIM:610563 ...
karyopherin subunit alpha 2 ELISA Kits | Biocompare
Compare karyopherin subunit alpha 2 ELISA Kits from leading suppliers on Biocompare. View specifications, prices, citations, ... karyopherin subunit alpha 2 ELISA Kits. The ELISA (enzyme-linked immunosorbent assay) is a widely used application for ... Provided below are ELISA kits targeting karyopherin subunit alpha 2, a human protein encoded by KPNA2. This protein is 529 ... Your search returned 37 karyopherin subunit alpha 2 ELISA Kit across 11 suppliers. ...
RCSB PDB
- 1BK6: KARYOPHERIN ALPHA (YEAST) + SV40 T ANTIGEN NLS Methods Report Page
3D View
- 1BK6: KARYOPHERIN ALPHA (YEAST) + SV40 T ANTIGEN NLS 3D View Report Page
RCSB PDB - 1EE5: YEAST KARYOPHERIN (IMPORTIN) ALPHA IN A COMPLEX WITH A NUCLEOPLASMIN NLS PEPTIDE
Karyopherin alpha (importin alpha) is an adaptor molecule that recognizes proteins containing nuclear localization signals ( ... Karyopherin alpha (importin alpha) is an adaptor molecule that recognizes proteins containing nuclear localization signals ( ... KARYOPHERIN ALPHA. A. 424. Saccharomyces cerevisiae. Mutation(s): 1 Find proteins for Q02821 (Saccharomyces cerevisiae (strain ... YEAST KARYOPHERIN (IMPORTIN) ALPHA IN A COMPLEX WITH A NUCLEOPLASMIN NLS PEPTIDE. *DOI: 10.2210/pdb1EE5/pdb ...
LOC365232 (similar to karyopherin (importin) alpha 4) - Rat Genome Database
WikiGenes - KPNA4 - karyopherin alpha 4 (importin alpha 3)
Synonyms: IPOA3, Importin alpha Q1, Importin subunit alpha-3, Karyopherin subunit alpha-4, MGC12217, ... ... Assignment of KPNA4 and KPNB1 encoding karyopherin alpha 4 and beta 1 to human chromosome bands 11q22 and 17q21 respectively, ... Assignment of KPNA4 and KPNB1 encoding karyopherin alpha 4 and beta 1 to human chromosome bands 11q22 and 17q21 respectively, ... Cloning of a cDNA encoding a novel importin-alpha homologue, Qip1: discrimination of Qip1 and Rch1 from hSrp1 by their ability ...
karyopherin alpha 6 CRISPR Plasmids | SCBT - Santa Cruz Biotechnology
offers a broad range of karyopherin alpha 6 CRISPR/Cas9 Knockout plasmids and karyopherin alpha 6 Double Nickase Plasmids. ... karyopherin alpha 6 gene silencers are available as karyopherin alpha 6 CRISPR/Cas9 Knockout plasmids and karyopherin alpha 6 ... karyopherin alpha 6 Antibodies for analysis of cellular responses to karyopherin alpha 6 CRISPR Products ... karyopherin alpha 6 CRISPR Knockout, HDR and Nickase Knockout Plasmids PRODUCT NAME. CATALOG #. Species. APPLICATIONS. MARKER. ...
Macrocyclic peptide inhibitors for the protein-protein interaction of Zaire Ebola virus protein 24 and karyopherin alpha 5 -...
Karyopherin alpha 5 | definition of karyopherin alpha 5 by Medical dictionary
What is karyopherin alpha 5? Meaning of karyopherin alpha 5 medical term. What does karyopherin alpha 5 mean? ... Looking for online definition of karyopherin alpha 5 in the Medical Dictionary? karyopherin alpha 5 explanation free. ... redirected from karyopherin alpha 5) KPNA5. A gene on chromosome 6q22.1 that encodes an alpha subunit of karyopherin, which ... Karyopherin alpha 5 , definition of karyopherin alpha 5 by Medical dictionary https://medical-dictionary.thefreedictionary.com/ ...
KPNA2 (karyopherin (importin) alpha 2) - KOMP (Knockout Mouse Project)
Vega: OTTMUSG3580 (Kpna2, karyopherin (importin) alpha 2)*CCDS: 25565, 25565.1*OMIM: KARYOPHERIN ALPHA-2; KPNA2*Gene Ontology: ... karyopherin (importin) alpha 2. Synonyms: 2410044B12Rik, importin, Importin alpha, importin alpha 1, IPOA1, m-importin, m- ... importin-alpha-P1, pendulin, Rch1. Gene nomenclature, locus information, and GO, OMIM, and PMID associations are updated daily ...
Targeting Nuclear Import Shuttles, Importins/Karyopherins Alpha by a Peptide Mimicking the NFκB1/p50 Nuclear Localization...
... with endogenous human importins/karyopherins to determine the mechanism of NTM modulation of importin alpha-mediated nuclear ... Targeting Nuclear Import Shuttles, Importins/Karyopherins Alpha by a Peptide Mimicking the NFκB1/p50 Nuclear Localization ... with endogenous human importins/karyopherins to determine the mechanism of NTM modulation of importin alpha-mediated nuclear ...
Die nukleäre Expression von Karyopherin alpha 2: ein prädiktiver Faktor für das Überleben von Patientinnen mit...
Meiler, Robert Paul (2010) Die nukleäre Expression von Karyopherin alpha 2: ein prädiktiver Faktor für das Überleben von ... Die nukleäre Expression von Karyopherin alpha 2: ein prädiktiver Faktor für das Überleben von Patientinnen mit ... Karyopherin a2 (KPNA2) has been reported as a important factor of tumorgenesis and progression of breast cancer. The aim of ... Karyopherin α2 (KPNA2) stellte sich als ein wichtiger Faktor bezüglich Tumorentstehung und Fortschreiten des Mammakarzinoms dar ...
Karyopherin alpha 2 is a novel prognostic marker and a potential therapeutic target for colon cancer | Journal of Experimental ...
... a member of the karyopherin family, plays a vital role in carcinogenesis. Yet its role in colon cancer is poorly characterized ... Karyopherin alpha 2 (KPNA2), a member of the karyopherin family, plays a vital role in carcinogenesis. Yet its role in colon ... Karyopherin alpha 2 is a novel prognostic marker and a potential therapeutic target for colon cancer. *Yu Zhang1. , ... Hu ZY, Yuan SX, Yang Y, Zhou WP, Jiang H. Pleomorphic adenoma gene 1 mediates the role of karyopherin alpha 2 and has ...
KPNA6 Gene - GeneCards | IMA7 Protein | IMA7 Antibody
Karyopherin Subunit Alpha 6, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The ... a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in ... KPNA6 (Karyopherin Subunit Alpha 6) is a Protein Coding gene. Diseases associated with KPNA6 include Lennox-Gastaut Syndrome. ... Acetylation of importin-alpha nuclear import factors by CBP/p300. (PMID: 10801418) Bannister AJ … Kouzarides T (Current biology ...
KPNA2 Gene - GeneCards | IMA1 Protein | IMA1 Antibody
Karyopherin Subunit Alpha 2, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The ... Karyopherin alpha 2 (RAG cohort 1, importin alpha 1) (Q6NVW7_HUMAN). *Karyopherin alpha 2 (RAG cohort 1, importin alpha 1) ( ... Mammalian karyopherin alpha 1 beta and alpha 2 beta heterodimers: alpha 1 or alpha 2 subunit binds nuclear localization signal ... karyopherin (importin) alpha 2,56kDa,component of the nuclear protein complex (NPC),yeast SRP1 and Xenopus importin homolog, ...
beta Karyopherins
- Importin-beta
Summary Report | CureHunter
Once inside the CELL NUCLEUS beta karyopherins interact with RAN GTP-BINDING PROTEIN and dissociate from alpha karyopherins. ... Nucleocytoplasmic transport molecules that bind to ALPHA KARYOPHERINS in the CYTOSOL and are involved in transport of molecules ... Beta karyopherins bound to RAN GTP-BINDING PROTEIN are then re-transported to the cytoplasm where hydrolysis of the GTP of RAN ... GTP-BINDING PROTEIN causes release of karyopherin beta. ... formation by tethering karyopherin alpha 2 and karyopherin beta ...
Ebola virus VP24 targets a unique NLS binding site on karyopherin alpha 5 to selectively compete with nuclear import of...
Fingerprint Dive into the research topics of Ebola virus VP24 targets a unique NLS binding site on karyopherin alpha 5 to ... T1 - Ebola virus VP24 targets a unique NLS binding site on karyopherin alpha 5 to selectively compete with nuclear import of ... Ebola virus VP24 targets a unique NLS binding site on karyopherin alpha 5 to selectively compete with nuclear import of ... Ebola virus VP24 targets a unique NLS binding site on karyopherin alpha 5 to selectively compete with nuclear import of ...
Pleomorphic adenoma gene 1 mediates the role of karyopherin alpha 2 and has prognostic significance in hepatocellular carcinoma...
Karyopherin alpha 2 (KPNA2) promotes tumor growth in hepatocellular carcinoma (HCC). We aimed to determine the content and ... Karyopherin alpha 2 (KPNA2) is one of karyopherin a family, and could form heterodimer with Karyopherin 1 to promote nucleus ... Braem CV, Kas K, Meyen E, Debiec-Rychter M, Van De Ven WJ, Voz ML: Identification of a karyopherin alpha 2 recognition site in ... Karyopherin alpha 2 (KPNA2) promotes tumor growth in hepatocellular carcinoma (HCC). We aimed to determine the content and ...
KPNA2 - Importin subunit alpha-1 - Homo sapiens (Human) - KPNA2 gene & protein
At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta ... "Mammalian karyopherin alpha 1 beta and alpha 2 beta heterodimers: alpha 1 or alpha 2 subunit binds nuclear localization signal ... "Mammalian karyopherin alpha 1 beta and alpha 2 beta heterodimers: alpha 1 or alpha 2 subunit binds nuclear localization signal ... "Mammalian karyopherin alpha 1 beta and alpha 2 beta heterodimers: alpha 1 or alpha 2 subunit binds nuclear localization signal ...
Npap60/Nup50 is a tri-stable switch that stimulates importin-alpha:beta-mediated nuclear protein import
... by the importin-alpha:beta receptor. We now show that Npap60 (also called Nup50), a protein previously believed to be a ... structural component of the NPC, is a Ran binding protein and a cofactor for importin-alpha:be … ... alpha Karyopherins / genetics * alpha Karyopherins / metabolism* * beta Karyopherins / genetics * beta Karyopherins / ... The N terminus binds the C terminus of importin-alpha, while a central domain binds importin-beta. Npap60:importin-alpha:beta ...
The Host-Dependent Interaction of Alpha-Importins With Influenza PB2 Polymerase Subunit Is Required for Virus RNA Replication
alpha Karyopherins / chemistry * alpha Karyopherins / metabolism* Substances * Mutant Proteins * Nuclear Localization Signals ... Alpha-importin was identified as a PB2-associated factor by proteomic analyses. To study the relevance of this interaction for ... Human alpha importin isoforms associated efficiently to PB2 protein of an H3N2 human virus but bound to diminished and variable ... The Host-Dependent Interaction of Alpha-Importins With Influenza PB2 Polymerase Subunit Is Required for Virus RNA Replication ...
KPNA6 - Importin subunit alpha-7 - Homo sapiens (Human) - KPNA6 gene & protein
At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta ... "Ebola virus VP24 proteins inhibit the interaction of NPI-1 subfamily karyopherin alpha proteins with activated STAT1.". Reid S. ... Importin subunit alpha-7Add BLAST. 536. Amino acid modifications. Feature key. Position(s). DescriptionActions. Graphical view ... IPR024931, Importing_su_alpha. Pfami. View protein in Pfam. PF00514, Arm, 8 hits. PF16186, Arm_3, 1 hit. PF01749, IBB, 1 ...
KEGG BRITE: KEGG Orthology (KO) - Saccharomyces cerevisiae (budding yeast)
YNL189W SRP1; karyopherin alpha YLR347C KAP95; karyopherin beta YJR042W NUP85; Nup85p YMR047C NUP116; FG-nucleoporin NUP116 ... YML085C TUB1; alpha-tubulin TUB1 YML124C TUB3; alpha-tubulin TUB3 YFL037W TUB2; beta-tubulin YPL174C NIP100; Nip100p YHR129C ... YML085C TUB1; alpha-tubulin TUB1 YML124C TUB3; alpha-tubulin TUB3 YNL148C ALF1; Alf1p YHR172W SPC97; Spc97p YNL126W SPC98; ... YML085C TUB1; alpha-tubulin TUB1 YML124C TUB3; alpha-tubulin TUB3 YFL037W TUB2; beta-tubulin YLR212C TUB4; gamma-tubulin ...
1ee5 - Proteopedia, life in 3D
BACKGROUND: Karyopherin alpha (importin alpha) is an adaptor molecule that recognizes proteins containing nuclear localization ... YEAST KARYOPHERIN (IMPORTIN) ALPHA IN A COMPLEX WITH A NUCLEOPLASMIN NLS PEPTIDE. ... RESULTS: We report the crystal structures of two peptide complexes of yeast karyopherin alpha (Kapalpha): one with a human c- ... Karyopherin alpha): role for Srp1p and Sts1p in protein degradation. Mol Cell Biol. 2000 Aug;20(16):6062-73. PMID:10913188 ...
Kpna4 alimentary system gene expression
Karyopherins: potential biological elements involved in the delayed graft function in renal transplant recipients | BMC Medical...
Signal transducers and activators of transcription (STAT) utilize karyopherins-alpha (KPNA) for their passage from cytoplasm ... of karyopherin-mediated nuclear transport in the onset and development of DGF. Additionally, it reveals that karyopherins could ... which includes genes encoding for several subtypes of karyopherins, a group of proteins involved in nucleocytoplasmic transport ... Karyopherin-alpha (also known as importin-alpha) is an adaptor protein that recognizes the first discovered or classical NLS, ...
Nucleus-specific importin alpha proteins and nucleoporins regulate protein import and nuclear division in the binucleate...
thermophila encodes a large, karyopherin alpha-like protein family. The diagram in panel A depicts the conserved domains of ... Localization studies of 13 putative importin (imp) alpha- and 11 imp beta-like proteins revealed that imp alpha-like proteins ... These data suggest that micronucleus-specific proteins are transported by specific imp alpha adapters. The different imp alpha ... Nucleus-specific importin alpha proteins and nucleoporins regulate protein import and nuclear division in the binucleate ...
Subunit alpha8
- Importin subunit alpha-1 is a protein that in humans is encoded by the KPNA1 gene. (wikipedia.org)
- Proteins involved in the first step of nuclear import are members of the alpha importin family of karyopherins such as importin subunit alpha-2. (wikipedia.org)
- Provided below are ELISA kits targeting karyopherin subunit alpha 2, a human protein encoded by KPNA2. (biocompare.com)
- Other alias names for this protein include: importin subunit alpha-1, RAG cohort protein 1, importin subunit alpha-2, importin-alpha-P1, pendulin, IPOA1, and QIP2. (biocompare.com)
- Your search returned 37 karyopherin subunit alpha 2 ELISA Kit across 11 suppliers. (biocompare.com)
- KPNA6 (Karyopherin Subunit Alpha 6) is a Protein Coding gene. (genecards.org)
- KPNA6 ( Karyopherin subunit alpha 6 ) هوَ بروتين يُشَفر بواسطة جين KPNA6 في الإنسان . (wikipedia.org)
- Differential gene expression analysis suggested that sorafenib resistance correlated with high karyopherin subunit alpha 3 (KPNA3) expression. (jcancer.org)
Protein26
- Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. (nih.gov)
- The protein encoded by this gene is a member of the importin alpha family. (nih.gov)
- This protein is 529 amino acids long, with a mass of 57.9 kDa, and is a member of the Importin alpha family. (biocompare.com)
- A gene on chromosome 6q22.1 that encodes an alpha subunit of karyopherin, which plays a key role in nuclear protein import as an adapter protein for nuclear receptor KPNB1. (thefreedictionary.com)
- Once inside the CELL NUCLEUS beta karyopherins interact with RAN GTP-BINDING PROTEIN and dissociate from alpha karyopherins. (curehunter.com)
- Beta karyopherins bound to RAN GTP-BINDING PROTEIN are then re-transported to the cytoplasm where hydrolysis of the GTP of RAN GTP-BINDING PROTEIN causes release of karyopherin beta. (curehunter.com)
- Karyopherin alpha 2 (KPNA2) is one of karyopherin a family, and could form heterodimer with Karyopherin 1 to promote nucleus protein import as an adapter protein [ 7 ]. (biomedcentral.com)
- We now show that Npap60 (also called Nup50), a protein previously believed to be a structural component of the NPC, is a Ran binding protein and a cofactor for importin-alpha:beta-mediated import. (nih.gov)
- Human alpha importin isoforms associated efficiently to PB2 protein of an H3N2 human virus but bound to diminished and variable extents to PB2 from H5N1 avian or human strains, suggesting that the function of alpha importin during RNA replication is important for the adaptation of avian viruses to the human host. (nih.gov)
- Tabb MM, Tongaonkar P, Vu L, Nomura M. Evidence for separable functions of Srp1p, the yeast homolog of importin alpha (Karyopherin alpha): role for Srp1p and Sts1p in protein degradation. (proteopedia.org)
- Nucleus-specific importin alpha proteins and nucleoporins regulate protein import and nuclear division in the binucleate Tetrahymena thermophila. (nih.gov)
- Disruption of the IMA10 gene encoding an imp alpha-like protein that accumulates in dividing micronuclei results in nuclear division defects and lethality. (nih.gov)
- Thus, nucleus-specific protein import and nuclear function in Tetrahymena are regulated by diverse, specialized karyopherins. (nih.gov)
- The protein encoded by this gene belongs to the importin alpha family, and is involved in nuclear protein import. (genetex.com)
- KPNA5 protein belongs to the importin alpha protein family and is thought to be involved in NLS-dependent protein import into the nucleus.The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size. (acris-antibodies.com)
- KPNA5 protein belongs to the importin alpha protein family and is thought to be involved in NLS-dependent protein import into the nucleus. (acris-antibodies.com)
- Karyopherins shuttle their NLS-containing cargo through nuclear pore complexes (NPCs), protein channels that permeate the nuclear membrane. (asm.org)
- The imp α proteins have a characteristic structure consisting of an amino (N)-terminal imp β-binding (IBB) domain of ∼100 amino acids (aa) that is followed by 8 to 10 alpha-helical armadillo (ARM) repeats that span most of the remainder of the protein. (asm.org)
- Dies wurde in vitro durch Bindung des Adapterproteins Karyopherin-alpha an CKII-phosphoryliertes Terminales Protein bestätigt. (hu-berlin.de)
- Moreover, its functionality was depended on CKII activity that was verified by in vitro binding experiments of terminal protein to the import adaptor karyopherin-alpha. (hu-berlin.de)
- Many nuclear proteins possess a nuclear localisation signal (NLS) that is recognised by the importer protein importin-alpha. (eu.org)
- Importin-alpha is an adaptor protein for importin-beta which interacts with nuclear pore components to effect transport into the nucleus. (eu.org)
- In addition to binding plant karyopherin α, VirE3 interacts with VirE2, a major bacterial protein that directly associates with the T‐DNA and facilitates its nuclear import. (deepdyve.com)
- Karyopherin α2 was first identified as Rch-1, an NLS receptor which interacts with the RAG-1 recombination-activating protein in developing B and T cells. (bdbiosciences.com)
- A nucleocytoplasmic transport protein that binds to ALPHA KARYOPHERINS and RAN GTP BINDING PROTEIN inside the CELL NUCLEUS and participates in their export into CYTOPLASM. (curehunter.com)
- Moroianu J, Blobel G, Radu A. Nuclear protein import: Ran-GTP dissociates the karyopherin alphabeta heterodimer by displacing alpha from an overlapping binding site on beta. (proteopedia.org)
KPNA25
- KPNA2 also may play a role in V(D)J recombination Karyopherin alpha 2 has been shown to interact with: ARL4A ITK, KPNB1, PLAG1, RECQL, and SGK. (wikipedia.org)
- Karyopherin alpha 2 (KPNA2), a member of the karyopherin family, plays a vital role in carcinogenesis. (biomedcentral.com)
- KPNA2 is a member of the karyopherin α-family, also known as importin α-1 or RAG cohort 1, weighs about 58 kDa and consist of 529 amino acids [ 5 ]. (biomedcentral.com)
- Karyopherin alpha 2 (KPNA2) promotes tumor growth in hepatocellular carcinoma (HCC). (biomedcentral.com)
- Recent studies show that Karyopherin alpha 2 (KPNA2) is up-regulated in quite a number of cancers and associated with poor prognosis. (pubmedcentralcanada.ca)
Nucleus12
- Karyopherins act as carrier proteins between the cytoplasm and the nucleus. (biomedcentral.com)
- At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. (genecards.org)
- Although some molecules are able to diffuse through the large Nucleus Pore Complexes (NPCs) in the nucleus membrane, factors larger than 45 kDa including that associated with malignant diseases need to be mediated by karyopherin to import into the nucleus [ 6 ]. (biomedcentral.com)
- Interestingly, the most up-regulated pathway at both time points was NLS-bearing substrate import into nucleus , which includes genes encoding for several subtypes of karyopherins, a group of proteins involved in nucleocytoplasmic transport. (biomedcentral.com)
- Signal transducers and activators of transcription (STAT) utilize karyopherins-alpha (KPNA) for their passage from cytoplasm into the nucleus. (biomedcentral.com)
- Localization studies of 13 putative importin (imp) alpha- and 11 imp beta-like proteins revealed that imp alpha-like proteins are nucleus specific--nine localized to the germ line micronucleus--but that most imp beta-like proteins localized to both types of nuclei. (nih.gov)
- After their synthesis, TFs are transported into the nucleus a process regulated by karyopherins commonly known as importin alpha and β. (plantphysiol.org)
- We have shown that 20S core particles are translocated into the nucleus as inactive precursor complexes via the classical karyopherin alpha/beta import pathway (Lehmann et al. (hu-berlin.de)
- The importin-β family of transport receptors, also called karyopherins, or more specifically, importins or exportins, carries cargoes both into and out of the nucleus. (genetics.org)
- Here, we demonstrate that VirE3 is transferred from Agrobacterium to the plant cell and then imported into its nucleus via the karyopherin α‐dependent pathway. (deepdyve.com)
- Our data indicate that VirE3 can mimic this VIP1 function, acting as an 'adapter' molecule between VirE2 and karyopherin α and 'piggy‐backing' VirE2 into the host cell nucleus. (deepdyve.com)
- Most proteins imported into the nucleus contain a classical nuclear localization signal (cNLS) sequence that is recognized by members of the karyopherin-α (also known as KPNA or importin-α) family and mediates their nuclear import. (sciencemag.org)
KPNA2
- Summary During antiviral defense, interferon (IFN) signaling triggers nuclear transport of tyrosine-phosphorylated STAT1 (PY-STAT1), which occurs via a subset of karyopherin alpha (KPNA) nuclear transporters. (elsevier.com)
- A, Immunoblotting with Flag antibody in HEK293T cells expressing either one of 7 members of Flag-tagged karyopherin alpha (KPNA) family (top left). (aacrjournals.org)
Binds5
- Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. (nih.gov)
- The N terminus binds the C terminus of importin-alpha, while a central domain binds importin-beta. (nih.gov)
- Npap60:importin-alpha:beta binds cargo and can stimulate nuclear import. (nih.gov)
- The monopartite nuclear localisation signal (mNLS) binds to the major site of importin-alpha. (eu.org)
- Nevertheless, it is clear that most proteins do specify their own import signal and, of these, the vast majority have an NLS that binds to importin-alpha (also termed karyopherin-alpha). (eu.org)
Antibody2
- Immunohistochemical analysis of paraffin-embedded KYSE170 xenograft, using karyopherin alpha 3(GTX106324) antibody at 1:500 dilution. (genetex.com)
- Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10,000 dilution of the mouse anti-karyopherin antibody. (bdbiosciences.com)
KPNB12
- Karyopherin alpha 1 has been shown to interact with KPNB1 and UBR5. (wikipedia.org)
- Because RAN is a small GTPase that regulates nuclear transport controlled by karyopherin subunit beta 1 (KPNB1), RAN was further analyzed together with KPNB1. (frontiersin.org)
Classical karyopherin1
- Compared with wild-type, passive export rates of a classical karyopherin/importin (Kap) Kap60p/Kap95p-targeted NLS-GFP reporter (cNLS-GFP) were significantly faster in nup188- Δ and nup170- Δ cells. (rupress.org)
Heterodimer1
- The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. (nih.gov)
Antigen1
- Antigen standard for karyopherin alpha 3 (importin alpha 4) (KPNA3) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. (creativebiomart.net)
20021
- Für 20S Proteasomen konnten Lehmann und Mitarbeitende (2002, JMB, 317, 401) zeigen, dass Vorläuferkomplexe über den Karyopherin alpha/beta abhängigen Importweg in den Zellkern gelangen und dort zu 20S Proteasomen heranreifen. (hu-berlin.de)
CYTOSOL2
- Nucleocytoplasmic transport molecules that bind to ALPHA KARYOPHERINS in the CYTOSOL and are involved in transport of molecules through the NUCLEAR PORE COMPLEX. (curehunter.com)
- Importin-alpha must be returned to the cytosol to repeat the process. (eu.org)
Signals5
- Karyopherin alpha (importin alpha) is an adaptor molecule that recognizes proteins containing nuclear localization signals (NLSs). (rcsb.org)
- Crystallographic analysis of the specific yet versatile recognition of distinct nuclear localization signals by karyopherin alpha. (proteopedia.org)
- Conti E, Kuriyan J. Crystallographic analysis of the specific yet versatile recognition of distinct nuclear localization signals by karyopherin alpha. (proteopedia.org)
- The import of proteins containing nuclear localization signals (NLSs) is facilitated by karyopherin proteins, also known as importins. (asm.org)
- Many cargoes destined for nuclear import carry nuclear localization signals that are recognized by karyopherins (Kaps). (rupress.org)
Importin alpha family1
- Belongs to the importin alpha family. (abcam.com)
Receptors2
- Tumorigenesis and tumor progression are associated with dysfunction of the nuclear transport machinery at the level of import and export receptors (karyopherins) [ 3 ]. (biomedcentral.com)
- This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. (antibodies-online.com)
Antibodies1
- A molecular docking and dynamics study concluded that R521C and R521H mutations in FUS (show FUS Antibodies ) result in weak binding with Karyopherin-beta2 leading to amyotrophic lateral sclerosis. (antibodies-online.com)
Adaptor1
- Importin-alpha is itself an adaptor for the nuclear transport receptor importin-beta. (eu.org)
YEAST2
- RESULTS: We report the crystal structures of two peptide complexes of yeast karyopherin alpha (Kapalpha): one with a human c-myc NLS peptide, determined at 2.1 A resolution, and one with a Xenopus nucleoplasmin NLS peptide, determined at 2.4 A resolution. (proteopedia.org)
- In yeast and humans, which contain at least 14 and 20 karyopherins, respectively, most are members of the imp β family. (asm.org)
Complexes1
- Here, we provide evidence that nuclear import of base and lid complexes also depends on karyopherin alpha/beta. (hu-berlin.de)
Beta8
- Interestingly, the arginine-rich NLS of Rex uses importin beta for import but does so by a mechanism that is importin alpha independent. (curehunter.com)
- Many nuclear-targeted proteins are transported through the nuclear pore complex (NPC) by the importin-alpha:beta receptor. (nih.gov)
- These results identify Npap60 as a cofactor for importin-alpha:beta nuclear import and as a previously unidentified subunit of the importin complex. (nih.gov)
- There are two subfamilies of karyopherins: importin alpha and importin beta ( Tamura and Hara-Nishimura, 2014 ). (plantphysiol.org)
- In dieser Arbeit wird gezeigt, dass die nukleäre Lokalisation der 19S Regulatorkomplexe ebenfalls von Karyopherin alpha/beta abhängt. (hu-berlin.de)
- Unsere Daten unterstützen ein Modell wonach die nukleären 26S Proteasomen aus Subkomplexen zusammengelagert werden, die durch Karyopherin alpha/beta in den Zellkern gelangen. (hu-berlin.de)
- Our data support the model by which nuclear 26S proteasomes are assembled from subcomplexes imported by karyopherin alpha/beta. (hu-berlin.de)
- Finally, binding of RanGTP to importin-beta drives cargo release, with the importin-alpha still being bound to nucleoporins located on the nucleoplasmic side. (eu.org)
Family2
- Importin-alpha constitutes a multiprotein family in Metazoa with generally similar but perhaps not identical binding specificities ( Mason,2009 ). (eu.org)
- Heterodimers belonging to a highly conserved family of proteins called karyopherins are required for successful nuclear localization of cytosolic proteins. (bdbiosciences.com)
PEPTIDE1
- The authors analyzed interaction of a hydrophobic peptide, derived from the bifunctional nuclear transport modifier (NTM) peptide, with endogenous human importins/karyopherins to determine the mechanism of NTM modulation of importin alpha-mediated nuclear transport. (moleculardevices.com)
Interact1
- To identify the import machineries that interact with these different pores, we characterized the large families of karyopherin homologs encoded within the genome. (nih.gov)
Metabolism1
- Akt1/PKB alpha plays an important role in cell proliferation and cell metabolism. (antibodies-online.com)
Bind1
- In plakoglobin arm repeats bind alpha-catenin and N-cadherin. (embl-heidelberg.de)
Human3
- 1996). "Role of the karyopherin pathway in human immunodeficiency virus type 1 nuclear import" . (wikipedia.org)
- Recombinant Human Importin alpha 5. (novusbio.com)
- High-efficiency synthesis of human alpha-endorphin and magainin in the erythrocytes of transgenic mice: a production system for therapeutic peptides. (labome.org)
Import2
- Karyopherins and nuclear import. (nih.gov)
- Nup53p and Nup59p (but not Nup170p) contain FG repeats that act as binding sites for the import karyopherin, Kap121p. (rupress.org)