Enzymes that hydrolyze O-glucosyl-compounds. (Enzyme Nomenclature, 1992) EC 3.2.1.-.
An enzyme that catalyzes the endohydrolysis of 1,6-alpha-glucosidic linkages in isomaltose and dextrins produced from starch and glycogen by ALPHA-AMYLASES. EC 3.2.1.10.
Enzymes that catalyze the exohydrolysis of 1,4-alpha-glucosidic linkages with release of alpha-glucose. Deficiency of alpha-1,4-glucosidase may cause GLYCOGEN STORAGE DISEASE TYPE II.
An alpha-glucosidase inhibitor with antiviral action. Derivatives of deoxynojirimycin may have anti-HIV activity.
These compounds function as activated glycosyl carriers in the biosynthesis of glycoproteins and glycophospholipids. Include the pyrophosphates.
Carbohydrates consisting of between two (DISACCHARIDES) and ten MONOSACCHARIDES connected by either an alpha- or beta-glycosidic link. They are found throughout nature in both the free and bound form.
An indolizidine alkaloid from the plant Swainsona canescens that is a potent alpha-mannosidase inhibitor. Swainsonine also exhibits antimetastatic, antiproliferative, and immunomodulatory activity.
An exocellulase with specificity for a variety of beta-D-glycoside substrates. It catalyzes the hydrolysis of terminal non-reducing residues in beta-D-glucosides with release of GLUCOSE.
A lectin found in ENDOPLASMIC RETICULUM membranes that binds to specific N-linked OLIGOSACCHARIDES found on newly synthesized proteins. It may play role in PROTEIN FOLDING or retention and degradation of misfolded proteins in the endoplasmic reticulum.
Organic nitrogenous bases. Many alkaloids of medical importance occur in the animal and vegetable kingdoms, and some have been synthesized. (Grant & Hackh's Chemical Dictionary, 5th ed)
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
A system of cisternae in the CYTOPLASM of many cells. In places the endoplasmic reticulum is continuous with the plasma membrane (CELL MEMBRANE) or outer membrane of the nuclear envelope. If the outer surfaces of the endoplasmic reticulum membranes are coated with ribosomes, the endoplasmic reticulum is said to be rough-surfaced (ENDOPLASMIC RETICULUM, ROUGH); otherwise it is said to be smooth-surfaced (ENDOPLASMIC RETICULUM, SMOOTH). (King & Stansfield, A Dictionary of Genetics, 4th ed)
The chemical or biochemical addition of carbohydrate or glycosyl groups to other chemicals, especially peptides or proteins. Glycosyl transferases are used in this biochemical reaction.
Plasma glycoprotein member of the serpin superfamily which inhibits TRYPSIN; NEUTROPHIL ELASTASE; and other PROTEOLYTIC ENZYMES.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.
The rate dynamics in chemical or physical systems.
One of the two major pharmacological subdivisions of adrenergic receptors that were originally defined by the relative potencies of various adrenergic compounds. The alpha receptors were initially described as excitatory receptors that post-junctionally stimulate SMOOTH MUSCLE contraction. However, further analysis has revealed a more complex picture involving several alpha receptor subtypes and their involvement in feedback regulation.
Hypoxia-inducible factor 1, alpha subunit is a basic helix-loop-helix transcription factor that is regulated by OXYGEN availability and is targeted for degradation by VHL TUMOR SUPPRESSOR PROTEIN.
A member of the NICOTINIC ACETYLCHOLINE RECEPTOR subfamily of the LIGAND-GATED ION CHANNEL family. It consists entirely of pentameric a7 subunits expressed in the CNS, autonomic nervous system, vascular system, lymphocytes and spleen.
Cell surface receptor for LAMININ, epiligrin, FIBRONECTINS, entactin, and COLLAGEN. Integrin alpha3beta1 is the major integrin present in EPITHELIAL CELLS, where it plays a role in the assembly of BASEMENT MEMBRANE as well as in cell migration, and may regulate the functions of other integrins. Two alternatively spliced isoforms of the alpha subunit (INTEGRIN ALPHA3), are differentially expressed in different cell types.
An integrin alpha subunit that is unique in that it does not contain an I domain, and its proteolytic cleavage site is near the middle of the extracellular portion of the polypeptide rather than close to the membrane as in other integrin alpha subunits.
An integrin alpha subunit that primarily associates with INTEGRIN BETA1 or INTEGRIN BETA4 to form laminin-binding heterodimers. Integrin alpha6 has two alternatively spliced isoforms: integrin alpha6A and integrin alpha6B, which differ in their cytoplasmic domains and are regulated in a tissue-specific and developmental stage-specific manner.
An integrin found in FIBROBLASTS; PLATELETS; MONOCYTES, and LYMPHOCYTES. Integrin alpha5beta1 is the classical receptor for FIBRONECTIN, but it also functions as a receptor for LAMININ and several other EXTRACELLULAR MATRIX PROTEINS.
Integrin alpha4beta1 is a FIBRONECTIN and VCAM-1 receptor present on LYMPHOCYTES; MONOCYTES; EOSINOPHILS; NK CELLS and thymocytes. It is involved in both cell-cell and cell- EXTRACELLULAR MATRIX adhesion and plays a role in INFLAMMATION, hematopoietic cell homing and immune function, and has been implicated in skeletal MYOGENESIS; NEURAL CREST migration and proliferation, lymphocyte maturation and morphogenesis of the PLACENTA and HEART.
An interleukin-1 subtype that occurs as a membrane-bound pro-protein form that is cleaved by proteases to form a secreted mature form. Unlike INTERLEUKIN-1BETA both membrane-bound and secreted forms of interleukin-1alpha are biologically active.
An integrin found on fibroblasts, platelets, endothelial and epithelial cells, and lymphocytes where it functions as a receptor for COLLAGEN and LAMININ. Although originally referred to as the collagen receptor, it is one of several receptors for collagen. Ligand binding to integrin alpha2beta1 triggers a cascade of intracellular signaling, including activation of p38 MAP kinase.
A subclass of alpha-adrenergic receptors that mediate contraction of SMOOTH MUSCLE in a variety of tissues such as ARTERIOLES; VEINS; and the UTERUS. They are usually found on postsynaptic membranes and signal through GQ-G11 G-PROTEINS.
This integrin alpha subunit combines with INTEGRIN BETA1 to form a receptor (INTEGRIN ALPHA5BETA1) that binds FIBRONECTIN and LAMININ. It undergoes posttranslational cleavage into a heavy and a light chain that are connected by disulfide bonds.
Integrin alpha1beta1 functions as a receptor for LAMININ and COLLAGEN. It is widely expressed during development, but in the adult is the predominant laminin receptor (RECEPTORS, LAMININ) in mature SMOOTH MUSCLE CELLS, where it is important for maintenance of the differentiated phenotype of these cells. Integrin alpha1beta1 is also found in LYMPHOCYTES and microvascular endothelial cells, and may play a role in angiogenesis. In SCHWANN CELLS and neural crest cells, it is involved in cell migration. Integrin alpha1beta1 is also known as VLA-1 and CD49a-CD29.
A subclass of alpha-adrenergic receptors found on both presynaptic and postsynaptic membranes where they signal through Gi-Go G-PROTEINS. While postsynaptic alpha-2 receptors play a traditional role in mediating the effects of ADRENERGIC AGONISTS, the subset of alpha-2 receptors found on presynaptic membranes signal the feedback inhibition of NEUROTRANSMITTER release.
A cell surface receptor mediating cell adhesion to the EXTRACELLULAR MATRIX and to other cells via binding to LAMININ. It is involved in cell migration, embryonic development, leukocyte activation and tumor cell invasiveness. Integrin alpha6beta1 is the major laminin receptor on PLATELETS; LEUKOCYTES; and many EPITHELIAL CELLS, and ligand binding may activate a number of signal transduction pathways. Alternative splicing of the cytoplasmic domain of the alpha6 subunit (INTEGRIN ALPHA6) results in the formation of A and B isoforms of the heterodimer, which are expressed in a tissue-specific manner.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
This intrgrin is a key component of HEMIDESMOSOMES and is required for their formation and maintenance in epithelial cells. Integrin alpha6beta4 is also found on thymocytes, fibroblasts, and Schwann cells, where it functions as a laminin receptor (RECEPTORS, LAMININ) and is involved in wound healing, cell migration, and tumor invasiveness.
The alpha subunits of integrin heterodimers (INTEGRINS), which mediate ligand specificity. There are approximately 18 different alpha chains, exhibiting great sequence diversity; several chains are also spliced into alternative isoforms. They possess a long extracellular portion (1200 amino acids) containing a MIDAS (metal ion-dependent adhesion site) motif, and seven 60-amino acid tandem repeats, the last 4 of which form EF HAND MOTIFS. The intracellular portion is short with the exception of INTEGRIN ALPHA4.
A family of transmembrane glycoproteins (MEMBRANE GLYCOPROTEINS) consisting of noncovalent heterodimers. They interact with a wide variety of ligands including EXTRACELLULAR MATRIX PROTEINS; COMPLEMENT, and other cells, while their intracellular domains interact with the CYTOSKELETON. The integrins consist of at least three identified families: the cytoadhesin receptors(RECEPTORS, CYTOADHESIN), the leukocyte adhesion receptors (RECEPTORS, LEUKOCYTE ADHESION), and the VERY LATE ANTIGEN RECEPTORS. Each family contains a common beta-subunit (INTEGRIN BETA CHAINS) combined with one or more distinct alpha-subunits (INTEGRIN ALPHA CHAINS). These receptors participate in cell-matrix and cell-cell adhesion in many physiologically important processes, including embryological development; HEMOSTASIS; THROMBOSIS; WOUND HEALING; immune and nonimmune defense mechanisms; and oncogenic transformation.
An integrin alpha subunit that binds COLLAGEN and LAMININ though its I domain. It combines with INTEGRIN BETA1 to form the heterodimer INTEGRIN ALPHA1BETA1.
Brain waves characterized by a relatively high voltage or amplitude and a frequency of 8-13 Hz. They constitute the majority of waves recorded by EEG registering the activity of the parietal and occipital lobes when the individual is awake, but relaxed with the eyes closed.
An integrin alpha subunit that occurs as alternatively spliced isoforms. The isoforms are differentially expressed in specific cell types and at specific developmental stages. Integrin alpha3 combines with INTEGRIN BETA1 to form INTEGRIN ALPHA3BETA1 which is a heterodimer found primarily in epithelial cells.
Deficiency of the protease inhibitor ALPHA 1-ANTITRYPSIN that manifests primarily as PULMONARY EMPHYSEMA and LIVER CIRRHOSIS.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
One of the two major classes of cholinergic receptors. Nicotinic receptors were originally distinguished by their preference for NICOTINE over MUSCARINE. They are generally divided into muscle-type and neuronal-type (previously ganglionic) based on pharmacology, and subunit composition of the receptors.
Drugs that selectively bind to and activate alpha adrenergic receptors.
A nuclear transcription factor. Heterodimerization with RETINOID X RECEPTOR GAMMA is important to metabolism of LIPIDS. It is the target of FIBRATES to control HYPERLIPIDEMIAS.
A naturally occurring prostaglandin that has oxytocic, luteolytic, and abortifacient activities. Due to its vasocontractile properties, the compound has a variety of other biological actions.
Drugs that bind to but do not activate alpha-adrenergic receptors thereby blocking the actions of endogenous or exogenous adrenergic agonists. Adrenergic alpha-antagonists are used in the treatment of hypertension, vasospasm, peripheral vascular disease, shock, and pheochromocytoma.
Proteins prepared by recombinant DNA technology.
Hepatocyte nuclear factor 1-alpha is a transcription factor found in the LIVER; PANCREAS; and KIDNEY that regulates HOMEOSTASIS of GLUCOSE.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.

Alternative splicing of transcripts encoding the alpha- and beta-subunits of mouse glucosidase II in T lymphocytes. (1/973)

Glucosidase II is a processing enzyme of the endoplasmic reticulum that functions to hydrolyze two glucose residues in immature N -linked oligosaccharides attached to newly synthesized polypeptides. We previously reported the cDNA cloning of the alpha- and beta-subunits of mouse glucosidase II from T cells following copurification of these proteins with the highly glycosylated transmembrane protein-tyrosine phosphatase CD45. Subsequent examination of additional cDNA clones, coupled with partial genomic DNA sequencing, has revealed that both subunits are encoded by gene products that undergo alternative splicing in T lymphocytes. The catalytic alpha-subunit possesses two variably expressed segments, box Alpha1, consisting of 22 amino acids located proximal to the amino-terminus, and box Alpha2, composed of 9 amino acids situated between the amino-terminus and the putative catalytic site in the central region of the molecule. Box Beta1, a variably expressed 7 amino acid segment in the beta-subunit of glucosidase II, is located immediately downstream of an acidic stretch near the carboxyl-terminus. Screening of reverse transcribed RNA by polymerase chain reaction confirms the variable inclusion of each of these segments in transcripts obtained from a panel of T-lymphocyte cell lines. Thus, distinct isoforms of glucosidase II exist that may perform specialized functions.  (+info)

The AcbC protein from Actinoplanes species is a C7-cyclitol synthase related to 3-dehydroquinate synthases and is involved in the biosynthesis of the alpha-glucosidase inhibitor acarbose. (2/973)

The putative biosynthetic gene cluster for the alpha-glucosidase inhibitor acarbose was identified in the producer Actinoplanes sp. 50/110 by cloning a DNA segment containing the conserved gene for dTDP-D-glucose 4,6-dehydratase, acbB. The two flanking genes were acbA (dTDP-D-glucose synthase) and acbC, encoding a protein with significant similarity to 3-dehydroquinate synthases (AroB proteins). The acbC gene was overexpressed heterologously in Streptomyces lividans 66, and the product was shown to be a C7-cyclitol synthase using sedo-heptulose 7-phosphate, but not ido-heptulose 7-phosphate, as its substrate. The cyclization product, 2-epi-5-epi-valiolone ((2S,3S,4S,5R)-5-(hydroxymethyl)cyclohexanon-2,3,4,5-tetrol), is a precursor of the valienamine moiety of acarbose. A possible five-step reaction mechanism is proposed for the cyclization reaction catalyzed by AcbC based on the recent analysis of the three-dimensional structure of a eukaryotic 3-dehydroquinate synthase domain (Carpenter, E. P., Hawkins, A. R., Frost, J. W., and Brown, K. A. (1998) Nature 394, 299-302).  (+info)

Trypanosoma cruzi calreticulin is a lectin that binds monoglucosylated oligosaccharides but not protein moieties of glycoproteins. (3/973)

Trypanosoma cruzi is a protozoan parasite that belongs to an early branch in evolution. Although it lacks several features of the pathway of protein N-glycosylation and oligosaccharide processing present in the endoplasmic reticulum of higher eukaryotes, it displays UDP-Glc:glycoprotein glucosyltransferase and glucosidase II activities. It is herewith reported that this protozoan also expresses a calreticulin-like molecule, the third component of the quality control of glycoprotein folding. No calnexin-encoding gene was detected. Recombinant T. cruzi calreticulin specifically recognized free monoglucosylated high-mannose-type oligosaccharides. Addition of anti-calreticulin serum to extracts obtained from cells pulse-chased with [35S]Met plus [35S]Cys immunoprecipitated two proteins that were identified as calreticulin and the lysosomal proteinase cruzipain (a major soluble glycoprotein). The latter but not the former protein disappeared from immunoprecipitates upon chasing cells. Contrary to what happens in mammalian cells, addition of the glucosidase II inhibitor 1-deoxynojirimycin promoted calreticulin-cruzipain interaction. This result is consistent with the known pathway of protein N-glycosylation and oligosaccharide processing occurring in T. cruzi. A treatment of the calreticulin-cruzipain complexes with endo-beta-N-acetylglucosaminidase H either before or after addition of anti-calreticulin serum completely disrupted calreticulin-cruzipain interaction. In addition, mature monoglucosylated but not unglucosylated cruzipain isolated from lysosomes was found to interact with recombinant calreticulin. It was concluded that the quality control of glycoprotein folding appeared early in evolution, and that T. cruzi calreticulin binds monoglucosylated oligosaccharides but not the protein moiety of cruzipain. Furthermore, evidence is presented indicating that glucosyltransferase glucosylated cruzipain at its last folding stages.  (+info)

Murine acid alpha-glucosidase: cell-specific mRNA differential expression during development and maturation. (4/973)

Acid alpha-glucosidase (GAA) cleaves the alpha1-4 and alpha1-6 glycosidic linkages of glycogen and related alpha-glucosyl substrates within lysosomes. Its deficiency results in glycogen storage disease type II (GSDII) variants including Pompe disease. To gain insight into the tissue patterns of involvement by glycogen storage in GSDII, GAA mRNA expression in mouse tissues was evaluated by Northern blot and in situ hybridization analyses. Extensive temporal and spatial variation of GAA mRNA was observed. During preterm maturation, GAA mRNA levels of whole mice progressively increased as assessed by Northern analysis. By in situ hybridization with GAA antisense mRNA, low signals were detected in most tissues throughout gestation. However, increased expression in specific cell types of different tissues was observed beginning at 16 days post coitum in developing brain neurons, primitive inner ear cells, and seminiferous tubular epithelium. In adult mice, whole-organ GAA mRNA levels were highest in brain, moderate in heart, liver, and skeletal muscle, and lowest in the series kidney > lung > testis > spleen. By in situ hybridization, the highest-intensity signals were in neurons of the central and peripheral nervous systems whereas neuroglial cells had only low-level signal. Signals of moderate intensity were in cardiomyocytes whereas low signals were in hepatocytes and skeletal muscle myocytes and very low in cells of the lungs, thymus, pancreas, spleen, and adrenal glands. However, testicular Sertoli cells and kidney tubular epithelial cells had significant signals even though surrounding cells had very low signals. The discrete temporal and spatial variations of GAA mRNA during development indicate different physiological roles for this enzyme in various cell types and developmental stages.  (+info)

Delayed symptom onset and increased life expectancy in Sandhoff disease mice treated with N-butyldeoxynojirimycin. (5/973)

Sandhoff disease is a neurodegenerative disorder resulting from the autosomal recessive inheritance of mutations in the HEXB gene, which encodes the beta-subunit of beta-hexosaminidase. GM2 ganglioside fails to be degraded and accumulates within lysosomes in cells of the periphery and the central nervous system (CNS). There are currently no therapies for the glycosphingolipid lysosomal storage diseases that involve CNS pathology, including the GM2 gangliosidoses. One strategy for treating this and related diseases is substrate deprivation. This would utilize an inhibitor of glycosphingolipid biosynthesis to balance synthesis with the impaired rate of catabolism, thus preventing storage. One such inhibitor is N-butyldeoxynojirimycin, which currently is in clinical trials for the potential treatment of type 1 Gaucher disease, a related disease that involves glycosphingolipid storage in peripheral tissues, but not in the CNS. In this study, we have evaluated whether this drug also could be applied to the treatment of diseases with CNS storage and pathology. We therefore have treated a mouse model of Sandhoff disease with the inhibitor N-butyldeoxynojirimycin. The treated mice have delayed symptom onset, reduced storage in the brain and peripheral tissues, and increased life expectancy. Substrate deprivation therefore offers a potentially general therapy for this family of lysosomal storage diseases, including those with CNS disease.  (+info)

Androgen regulation of glycosidase secretion in epithelial cell cultures from human epididymis. (6/973)

The human epididymis and its secretions actively promote sperm fertilizing capacity and provide protection for spermatozoa against harmful influences. Among epididymal secretions, glycosidases have been recently studied and associated with molecular changes on the sperm surface. In the present work, we studied the influence of different concentrations of testosterone, dihydrotestosterone and cyproterone acetate on the secretion of alpha-glucosidase, N-acetyl-glucosaminidase, beta-glucuronidase and alpha-mannosidase by isolated and cultured epithelial cells from human caput, corpus and cauda epididymides. Cell cultures were obtained from aggregates of isolated tubule fragments plated on extracellular matrix-covered multi-well plates. Activities of the glycosidases were measured in conditioned culture media and were higher in the distal regions of the epididymis. Testosterone and dihydrotestosterone significantly increase the enzyme secretion in a concentration-dependent manner. This increase was higher in corpus and/or cauda than in caput epididymis. Cyproterone acetate caused a dose-dependent decrease in glycosidase secretion in cultures from all epididymal regions. It is concluded that the secretion of epididymal glycosidases is regulated by androgen, being stimulated by dihydrotestosterone and testosterone and inhibited by the androgen antagonist cyproterone acetate.  (+info)

Coordinate transcriptional control in the hyperthermophilic archaeon Sulfolobus solfataricus. (7/973)

The existence of a global gene regulatory system in the hyperthermophilic archaeon Sulfolobus solfataricus is described. The system is responsive to carbon source quality and acts at the level of transcription to coordinate synthesis of three physically unlinked glycosyl hydrolases implicated in carbohydrate utilization. The specific activities of three enzymes, an alpha-glucosidase (malA), a beta-glycosidase (lacS), and an alpha-amylase, were reduced 4-, 20-, and 10-fold, respectively, in response to the addition of supplementary carbon sources to a minimal sucrose medium. Western blot analysis using anti-alpha-glucosidase and anti-beta-glycosidase antibodies indicated that reduced enzyme activities resulted exclusively from decreased enzyme levels. Northern blot analysis of malA and lacS mRNAs revealed that changes in enzyme abundance arose primarily from reductions in transcript concentrations. Culture conditions precipitating rapid changes in lacS gene expression were established to determine the response time of the regulatory system in vivo. Full induction occurred within a single generation whereas full repression occurred more slowly, requiring nearly 38 generations. Since lacS mRNA abundance changed much more rapidly in response to a nutrient down shift than to a nutrient up shift, transcript synthesis rather than degradation likely plays a role in the regulatory response.  (+info)

Purification and characterization of an alpha-glucosidase from Rhizobium sp. (Robinia pseudoacacia L.) strain USDA 4280. (8/973)

A novel alpha-glucosidase with an apparent subunit mass of 59 +/- 0. 5 kDa was purified from protein extracts of Rhizobium sp. strain USDA 4280, a nodulating strain of black locust (Robinia pseudoacacia L), and characterized. After purification to homogeneity (475-fold; yield, 18%) by ammonium sulfate precipitation, cation-exchange chromatography, hydrophobic chromatography, dye chromatography, and gel filtration, this enzyme had a pI of 4.75 +/- 0.05. The enzyme activity was optimal at pH 6.0 to 6.5 and 35 degrees C. The activity increased in the presence of NH4+ and K+ ions but was inhibited by Cu2+, Ag+, Hg+, and Fe2+ ions and by various phenyl, phenol, and flavonoid derivatives. Native enzyme activity was revealed by native gel electrophoresis and isoelectrofocusing-polyacrylamide gel electrophoresis with fluorescence detection in which 4-methylumbelliferyl alpha-glucoside was the fluorogenic substrate. The enzyme was more active with alpha-glucosides substituted with aromatic aglycones than with oligosaccharides. This alpha-glucosidase exhibited Michaelis-Menten kinetics with 4-methylumbelliferyl alpha-D-glucopyranoside (Km, 0.141 microM; Vmax, 6.79 micromol min-1 mg-1) and with p-nitrophenyl alpha-D-glucopyranoside (Km, 0.037 microM; Vmax, 2.92 micromol min-1 mg-1). Maltose, trehalose, and sucrose were also hydrolyzed by this enzyme.  (+info)

Recombinant human acid alpha-glucosidase is safe and effective for treatment of infantile-onset Pompe disease. Eleven patients experienced adverse events related to treatment, but none discontinued. The young age at which these patients initiated therapy may have contributed to their improved respon …
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Castilla, C.; Paris, H.; Murat, J.C., 1979: Evidence of a similar distribution of neutral alpha glucosidase ec 3.2.1.3 and glucose 6 phosphatase in sub cellular fractions of rat and trout liver
Global Lysosomal Alpha Glucosidase Sales Market Report 2017 is a market research report available at US $4000 for a Single User PDF License from RnR Market Research Reports Library.
Alpha-glucosidase inhibitors (AGIs) are a class of drugs used to treat type 2 diabetes. The key benefit of AGIs is that they do not cause hypoglycemia (low blood sugar) or weight gain and are taken orally. These drugs help lower blood sugar levels in the body by blocking the breakdown of starchy foods like bread and potatoes in the intestine and thus delaying the absorption of carbohydrates, according to the American Diabetes Association They also slow down the breakdown of some sugars, like table sugar. Because they work to slow digestion, AGIs are taken at the start of a meal. The alpha-glucosidase inhibitors available today (with brand names in parenthesis) are: acarbose (Precose) miglitol (Glyset) An ADA published article states that
Alpha-glucosidase (EC 3.2.1.20, maltase, glucoinvertase, glucosidosucrase, maltase-glucoamylase, alpha-glucopyranosidase, glucosidoinvertase, alpha-D-glucosidase, alpha-glucoside hydrolase, alpha-1,4-glucosidase, alpha-D-glucoside glucohydrolase) is a glucosidase located in the brush border of the small intestine that acts upon α(1→4) bonds. This is in contrast to beta-glucosidase. Alpha-glucosidase breaks down starch and disaccharides to glucose. Maltase, a similar enzyme that cleaves maltose, is nearly functionally equivalent. Other glucosidases include: Cellulase Beta-glucosidase Debranching enzyme Alpha-glucosidase hydrolyzes terminal non-reducing (1→4)-linked alpha-glucose residues to release a single alpha-glucose molecule. Alpha-glucosidase is a carbohydrate-hydrolase that releases alpha-glucose as opposed to beta-glucose. Beta-glucose residues can be released by glucoamylase, a functionally similar enzyme. The substrate selectivity of alpha-glucosidase is due to subsite affinities ...
This gene encodes the alpha subunit of glucosidase II and a member of the glycosyl hydrolase 31 family of proteins. The heterodimeric enzyme glucosidase II plays a role in protein folding and quality control by cleaving glucose residues from immature glycoproteins in the endoplasmic reticulum. Expression of the encoded protein is elevated in lung tumor tissue and in response to UV irradiation. Mutations in this gene cause autosomal-dominant polycystic kidney and liver disease. [provided by RefSeq, Jul 2016 ...
This gene encodes lysosomal alpha-glucosidase, which is essential for the degradation of glycogen to glucose in lysosomes. Different forms of acid alpha-glucosidase are obtained by proteolytic processing. Defects in this gene are the cause of glycogen storage disease II, also known as Pompe disease, which is an autosomal recessive disorder with a broad clinical spectrum. Three transcript variants encoding the same protein have been found for this gene.[6] ...
In general terms, skeletal muscle mass is maintained by a precise dynamic balance between protein synthesis and degradation. Even a small sustained decrease in synthesis or increase in protein breakdown can affect the equilibrium and lead to atrophy (Sandri, 2016). At a molecular level, the reduced rate of protein synthesis is associated with impaired signaling through mTORC1, a major regulator of this anabolic process. This study is the first attempt to systematically analyze mTOR signaling pathway in Pompe disease, an inherited deficiency of lysosomal acid alpha‐glucosidase, in which the primary defect-intralysosomal glycogen accumulation-leads to numerous secondary abnormalities including defective autophagy, aberrant mitochondria and calcium homeostasis, and severe muscle wasting (reviewed in Lim et al 2014). We have found that the basal activity of mTOR in Pompe muscle cells is reduced; that mTOR is less sensitive to starvation and refeeding; that the relationship between the mTOR ...
In general terms, skeletal muscle mass is maintained by a precise dynamic balance between protein synthesis and degradation. Even a small sustained decrease in synthesis or increase in protein breakdown can affect the equilibrium and lead to atrophy (Sandri, 2016). At a molecular level, the reduced rate of protein synthesis is associated with impaired signaling through mTORC1, a major regulator of this anabolic process. This study is the first attempt to systematically analyze mTOR signaling pathway in Pompe disease, an inherited deficiency of lysosomal acid alpha‐glucosidase, in which the primary defect-intralysosomal glycogen accumulation-leads to numerous secondary abnormalities including defective autophagy, aberrant mitochondria and calcium homeostasis, and severe muscle wasting (reviewed in Lim et al 2014). We have found that the basal activity of mTOR in Pompe muscle cells is reduced; that mTOR is less sensitive to starvation and refeeding; that the relationship between the mTOR ...
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Alpha-glucosidase inhibitors work by ambitious and reversible inhibition of those intestinal enzymes. They diminish the digestion of carbohydrates and delay glucose absorption. This happens in a smaller and moderate rise in blood glucose levels following meals, and efficiently throughout the day.. AGIs have exhibited in reducing post-meal blood sugars and therefore helping to lower HbA1c, especially while used in combination with other diabetes drugs.. ...
alpha Glucosidase II antibody [N1N2], N-term (glucosidase, alpha; neutral AB) for ICC/IF, IHC-P, WB. Anti-alpha Glucosidase II pAb (GTX102237) is tested in Human, Mouse samples. 100% Ab-Assurance.
Alpha-glucosidase inhibitors, a class of drugs also known as starch blockers, function by slowing the absorption of certain carbohydrates in the gastrointestinal tract.
An enzyme which removes the last 1,4-linked alpha-D-glucose residue from the nonreducing end of a long chain (or polymer) of such residues, making an al...
Im sorry Nancy, but they are two completely different enzymes, that catalyze two totally different reactions. The only similarities are that they are both acid hydrolases, and are present in lysosomes, but thats like saying a Kia and a Lexus are both cars. Acid phosphatase (EC 3.1.3.2) catalyzes the hydrolysis of esters of orthophosphoric acid. It also nydrolyses pyrophosphate compounds and acts as a transphosphorylase. It has an optimum pH of between 4 and 5. Acid phosphatase is mainly found in lysosomes. However there are also extralysosomal acid phosphatases found in endoplasmic reticulum. It is possible to differentiate the acid phosphatases by their sensitivity to various inhibitors. Acid phosphatase activity is high in spleen, kidney, liver, intestine, and adrenals. Acid maltase (EC 3.1.3.20), also know as acid alpha-d-glucosidase, in simple terms breaks down maltose to glucose. It has a optimal pH of 5. Acid maltase is present in liver kidney and brain. A deficiency of acid maltase ...
A Century of Diabetes Care Pump therapy Human insulin Insulin analogs First human treated NPH insulin Type Insulin therapy 1920 Type Diet Sulfonylureas Alpha-glucosidase Inhibitors Biguanide Glitazones Meglitinides Insulin therapy Type 1 Since the early 1920s the only therapy available for type 1 diabetes has been insulin. (Banting and Best discovered insulin 1921) Improvements in insulin delivery have included pump therapy (mid-1970s), transition from animal to human insulin (1980s)1979 and the introduction of rapid acting insulin analogs (1990s) Type 2 Altlhough insulin has been available for type 2 diabetes patients since the 1920s, the major development has been the introduction of a number of oral agents: Sulfonylureas, the first available oral hypoglycemic agents (1956) Metformin (biguanides), although available in Europe for many years, recently available in this country since 1995 Alpha glucosidase inhibitors (starch blockers), thiazolidinediones (glitazones) and megltinides, agents
Aims: This paper mentions of the investigation of the enzymes binding to Voglibose and the choice of the enzyme which is suitable for drug enzyme interaction as used in biosensing. Voglibose is an antidiabetic drug which is reactive and hence used in very low concentrations. The existing methods of analysis of this drug are associated with certain disadvantages. Hence there is a need to establish alternative and simpler method of analysis which could be based on biosensing. The paper focuses on identification of the enzyme binding to the drug Voglibose.. Study Design: In this work there are two enzyme namely alpha amylase and alpha glucosidase which are considered as the potential targets for drug enzyme interaction for the drug Voglibose. The study is based on two approaches. The first one is molecular docking process done to verify the inhibition activity of the enzyme by the drug and the next method is chemical analysis to confirm the results obtained in molecular docking.. Place and Duration ...
Neutral a-glucosidase (NAG) activity in human seminal plasma is an important indicator for epididymis functionality. In the present study, the classic World Health Organization (WHO) method has been adapted to enhance assay robustness. Changes include modified enzyme reaction buffer composition and usage of an alternative enzyme inhibitor for background correction (glucose instead of castanospermine). Both methods have been tested in parallel on 144 semen samples, obtained from 94 patients/donors and 50 vasectomized men (negative control), respectively. Passing-Bablok regression analysis demonstrated equal assay performance. In terms of assay validation, analytical specificity, detection limit, measuring range, precision, and cut-off values have been calculated. These data confirm that the adapted method is a reliable, improved tool for NAG analysis in human semen.
Neutral a-glucosidase (NAG) activity in human seminal plasma is an important indicator for epididymis functionality. In the present study, the classic World Health Organization (WHO) method has been adapted to enhance assay robustness. Changes include modified enzyme reaction buffer composition and usage of an alternative enzyme inhibitor for background correction (glucose instead of castanospermine). Both methods have been tested in parallel on 144 semen samples, obtained from 94 patients/donors and 50 vasectomized men (negative control), respectively. Passing-Bablok regression analysis demonstrated equal assay performance. In terms of assay validation, analytical specificity, detection limit, measuring range, precision, and cut-off values have been calculated. These data confirm that the adapted method is a reliable, improved tool for NAG analysis in human semen.
Professionals recommend that, most of hypoglycemic drugs may have a harmful effect on the body. Sulfonylurea and glinides can cause liver damage. Biguanides also lead to gastrointestinal reaction and lactic acidosis, or anaphylaxis and large cell anemia reaction for the little users. As to alpha glucosidase inhibitor, the main side effect is causing gastrointestinal reaction, and which may cause abdominal distension, stomachache, diarrhea and intestinal exhaust too much. Besides, it is reported that the drugs might lead to severe hepatic lesion. Insulin sensitizer can not only trigger liver damage3but also increase the blood volume and the burden of heart ...
Alpha glucosidase inhibitor --- ( Kwon, Y., Apostolidis, E., Labbe, R. and Shetty, K. (2007). Inhibition of Staphylococcus aureus by Phenolic Phytochemicals of Selected Clonal Herbs Species of Lamiaceae Family and Likely Mode of Action through Proline Oxidation. Food Biotechnology, 21(1), pp.71-89 ...
D,L-1,2-Anhydro-myo-inositol; Inhibits alpha-glucosidase activity; specific inhibitor of glucocerebrosidase in cultured cells ...
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In the present study a comparative investigation on the divergence of α-glucosidase (α-Gls) enzyme was performed. The complete amino acid sequences of different species as stated in the materials and methods were aligned. The mammalian α-Gls enzymes are heterodimeric proteins with α-glucosidic activity. The enzymes contain a region responsible for the catalytic site for an enzyme termed glucoamylase. The comparative results from the present investigation showed that human and chimpanzee α-glucosidases had the closest similarity, and may have come from the same ancestor. There are two conserve P-type domains in human MGAM located in positions 88-134 and 954-1000 [16], which is completely conserved in chimpanzee`s enzyme. These domains have some synonymous amino acid alterations in rat and mouse MGAM. Interestingly, these domains are absent in plants and bacterial counterparts. There are two catalytic aspartic acid residues in mammalian enzymes which due to their nucleophilic nature has a ...
Figure 6: Total phenolics and Antioxidant activity of long term stored apples from Grocery stores (Levels are maintained and in several cases increased with post-harvest storage) Figure 7: Long term stored grocery store varieties maintain and in some cases increase the alpha-glucosidase bioactive function in proportion to total phenolics and antioxidant activity. Table 1: Quercetin Content as Determined by HPLC: units: micrograms/gram peel) Water Extracts of Peel Ethanol Extracts of Peel Ginger gold 128 ± 5 142 ± 3 Honeycrisp 144 ± 3 554 ± 5 Gala 93 ± 2 68 ± 1 McIntosh 101 ± 2 534 ± 6 Jonagold 286 ± 5 213 ± 2 Empire 41 ± 1 327 ± 5 Braeburn 390 ± 3 419 ± 6 Golden delicious 84 ± 3 119 ± 3 Fuji 136 ± 2 74 ± 2 Red delicious 133 ± 3 171 ± 3 The peel samples have higher diabetes relevant alpha-glucosidase inhibitory activity , which the target of the current pharmaceutical acarabose for this early stages type 2 diabetes target (Figure 1& 5). All varieties have good baseline and ...
Voglibose is an alpha-glucosidase inhibitor used for lowering post-prandial blood glucose levels in people with diabetes mellitus. It is made in India by Ranbaxy Labs and sold under the trade name Volix.
Mgam - mouse gene knockout kit via CRISPR, 1 kit. |dl||dt|Kit Component:|/dt||dd|- |strong|KN310054G1|/strong|, Mgam gRNA vector 1 in |a href=http://www.origene.com/CRISPR-CAS9/Detail.
When programmed with yeast prepro-α-factor mRNA, the heterologous reticulocyte/dog pancreas translation system synthesizes two pheromone related polypeptides, a cytosolically located primary translation product (pp-α-Fcyt, 21 kDa) and a membrane-specific and multiply glycosylated e-factor precursor (pp-α-F3, 27.5 kDa). Glycosylation of the membrane specific pp-α-F3 species is competitively inhibited by synthetic peptides containing the consensus sequence Asn-Xaa-Thr as indicated by a shift of its molecular mass from 27.5 kDa to about 19.5 kDa (pp-α-F0), whereas the primary translation product pp-α-F cyt is not affected. Likewise, only the glycosylated pp-α-F3 structure is digested by Endo H yielding a polypeptide with a molecular mass between PP-α-F0 and pp-α-F cyt. These observations strongly suggest that the primary translation product is proteolytically processed during/on its translocation into the lumen of the microsomal vesicles. We believe that this proteolytic processing is due ...
An alpha-glucosidase test can reveal a secretory dysfunction of the epididymis and reduced quality of the semen caused by this disorder.
2QLY: Human intestinal maltase-glucoamylase: crystal structure of the N-terminal catalytic subunit and basis of inhibition and substrate specificity
Pompe disease is caused by a deficiency of a critical enzyme in the body called acid alpha glucosidase (GAA). Normally, GAA is used by the bodys cells to break down glycogen (a stored form of sugar) within specialized structures called lysosomes. In infants with severe cases of Pompe disease (called Classical Infantile Pompe disease), an excessive amount of glycogen accumulates and is stored in various tissues, especially heart, skeletal muscle, and liver, which prevents their normal function. This study being conducted to evaluate the safety and effectiveness of recombinant human acid alpha-glucosidase (rhGAA) as a potential enzyme replacement therapy for Pompe disease. Patients diagnosed with Classical Infantile Pompe disease who have a small, but inactive, amount of natural GAA enzyme present in their bodies (called Cross-Reacting Immunologic Material-Positive or CRIM (+) patients), will be studied ...
Pompe disease (also known as glycogen storage disease type II, GSD-II) is caused by a deficiency of a critical enzyme in the body called acid alpha-glucosidase (GAA). Normally, GAA is used by the bodys cells to break down glycogen (a stored form of sugar) within specialized structures called lysosomes. In patients with Pompe disease, an excessive amount of glycogen accumulates and is stored in various tissues, especially heart and skeletal muscle, which prevents their normal function. This study is being conducted to evaluate the safety and effectiveness of recombinant human acid alpha-glucosidase (rhGAA) as a potential enzyme replacement therapy for Pompe disease. Patients diagnosed with infantile-onset Pompe disease who are less than or equal to 6 months old will be studied ...
This report demonstrates that a single intravenous administration of a gene therapy vector can potentially result in the correction of all affected muscles in a mouse model of a human genetic muscle disease. These results were achieved by capitalizing both on the positive attributes of modified adenovirus-based vectoring systems and receptor-mediated lysosomal targeting of enzymes. The muscle disease treated, glycogen storage disease type II, is a lysosomal storage disorder that manifests as a progressive myopathy, secondary to massive glycogen accumulations in the skeletal and/or cardiac muscles of affected individuals. We demonstrated that a single intravenous administration of a modified Ad vector encoding human acid alpha-glucosidase (GAA) resulted in efficient hepatic transduction and secretion of high levels of the precursor GAA proenzyme into the plasma of treated animals. Subsequently, systemic distribution and uptake of the proenzyme into the skeletal and cardiac muscles of the GAA-knockout
Alglucosidase alfa contains an enzyme that naturally occurs in the body in healthy people. Some people lack this enzyme because of a genetic disorder. Alglucosidase alfa helps replace this missing enzyme in such people. Alglucosidase alfa is used to treat a glycogen storage disorder called Pompe disease, (also called...
Abstract of Dr. Amalfitanos proposal: Acid Maltase Deficiency (AMD, also known as Pompe Disease), is due to a patients inability to produce enough acid alpha glucosidase (GAA). This results in glycogen accumulation in the patients limb muscles, as well as cardiac muscles (in those patients affected by the early age onset (infantile) form of the disease). Based upon published results using animal models, intravenous infusion of recombinant rhGAA (Myozyme® or Lumizyme®) at current doses is not adequate to provide maximal improvement to so-treated AMD/Pompe patients. However, current production methods for recombinant proteins such as GAA may not be capable of producing enough GAA enzyme to support higher and more frequent dosing. Our multiple, previously published studies confirm that a single Gene Therapy treatment of most AMD/Pompe patients can potentially overcome this problem, by allowing for high level production of GAA from the livers of AMD/Pompe patients for a continuous period of ...
Glycogen storage disease type II or Pompe disease is a severe neuromuscular disorder caused by mutations in the lysosomal enzyme, acid α-glucosidase (GAA), which result in pathological accumulation of glycogen throughout the body. Enzyme replacement therapy is available for Pompe disease; however, it has limited efficacy, has high immunogenicity, and fails to correct pathological glycogen accumulation in nervous tissue and skeletal muscle. Using bioinformatics analysis and protein engineering, we developed transgenes encoding GAA that could be expressed and secreted by hepatocytes. Then, we used adeno-associated virus (AAV) vectors optimized for hepatic expression to deliver the GAA transgenes to Gaa knockout (Gaa−/−) mice, a model of Pompe disease. Therapeutic gene transfer to the liver rescued glycogen accumulation in muscle and the central nervous system, and ameliorated cardiac hypertrophy as well as muscle and respiratory dysfunction in the Gaa−/− mice; mouse survival was also ...
[Effect of Psidium guajava leaf extract on alpha-glucosidase activity in small intestine of diabetic mouse].: The GPL water-soluble extract possesses the potent
Pompe disease is an inherited disorder characterized by muscle weakness and breathing problems. In this disorder, affected individuals cannot break down glycogen due to a shortage of the enzyme acid alpha-glucosidase. Glycogen then builds up to toxic levels in the lysosomes, damaging the bodys organs and tissues. There are three types of Pompe disease: classic infantile-onset, non-classic infantile-onset, and late-onset. The classic infantile-onset form is characterized by muscle weakness, poor muscle tone, hepatomegaly, and heart defects. Affected infants begin to have symptoms within the first few months of life, and experience a failure to thrive. Non-classic infantile-onset is characterized by delayed motor skills and progressive muscle weakness. Affected individuals usually die early in childhood. Late-onset Pompe disease is much milder and less severe than the infantile-onset forms. It is characterized by progressive muscle weakness and breathing problems that may lead to respiratory ...
Pompe disease, also called glycogen storage disease type II (GSD-II), is an autosomal recessive disorder due to a deficiency of the lysosomal enzyme acid alpha-1,4-glucosidase (abbreviated GAA). The function of the GAA enzyme, also known as acid maltase, is to breakdown glycogen in the lysosome. Absent or reduced GAA activity results in accumulation of glycogen within the lysosome, particularly in muscle cells. GSD-II is divided into two forms; an infantile form and a juvenile/adult onset form. In individuals with the infantile form of Pompe disease there is less than 1% of normal enzymatic activity, whereas in the juvenile/adult onset form there is some residual enzymatic activity. In Pompe disease, affected infants are severely hypotonic and have cardiomegaly. In addition, patients may have an enlarged tongue. The disease is usually fatal within the first year of life due cardiorespiratory failure. The clinical presentation in the juvenile/adult onset form (onset after 12 months of age) is ...
by CAP , Feb 15, 2017 , Press Releases. CRANBURY, N.J. and SAN DIEGO, Feb. 15, 2017 (GLOBE NEWSWIRE) - Amicus Therapeutics (Nasdaq:FOLD), a global biotechnology company at the forefront of rare and orphan diseases, today presented new scientific findings and preclinical data on functional outcomes in an oral presentation and poster1 at the 13thAnnual WORLDSymposium™ in San Diego, CA. ATB200/AT2221 is a novel treatment paradigm that consists of ATB200, a unique recombinant human acid alpha-glucosidase (rhGAA) enzyme with optimized carbohydrate structures, particularly mannose-6 phosphate (M6P), to enhance uptake in muscles, co-administered with AT2221, a pharmacological chaperone designed to stabilize ERT in circulation.. ...
Pompe disease is a genetic condition that is caused by a specific gene in the body called GAA not working correctly. Normally, GAA makes an enzyme called alpha-glucosidase, also known as acid maltase. This enzyme breaks down glycogen in certain parts of cells called the lysosomes. Pompe disease is part of a larger group of about 50 conditions called lysosomal storage diseases, which all involve the lysosome not working properly. Glycogen is a type of carbohydrate, or nutrient, that needs to be broken down into a smaller form for our bodies to use. When GAA doesnt work, it cant make acid maltase and glycogen builds up in the cells instead of being broken down. Excess glycogen damages cells and causes progressive muscle weakness (myopathy), including the muscles needed for the body to move (skeletal muscle), the heart to pump (cardiac muscle), and the lungs to breathe (respiratory muscle). The symptoms of Pompe disease can start as early as the newborn period or even before birth, but some types ...
When the film Extraordinary Measures debuts on January 22, it will tell the story of one mans quest to obtain treatment for his children who suffer from a rare metabolic disorder called Pompe disease.. The real story began 20 years ago at Duke University Medical Center when pediatric geneticist Y.T. Chen, MD, PhD, began work on the first and only life-saving treatment for Pompe. In 2006, the FDA approved the use of Myozyme, which is manufactured and marketed by Genzyme Corporation, based on Dukes research. As a result, the children portrayed in the movie, and those who are living with Pompe worldwide, were treated with Myozyme and given their first fighting chance at life.. What Is Pompe Disease?. Pompe disease results when mutations occur in the gene that triggers the production of an enzyme called acid alpha-glucosidase (GAA). That enzyme is responsible for helping the body break down glycogen (sugar).. When it is absent or deficient the glycogen builds up in the bodys cells, damages ...
Patients with glycogen storage disease type II (GSDII, Pompe disease) suffer from progressive muscle weakness due to acid α-glucosidase deficiency. The disease is inherited as an autosomal recessive trait with a spectrum of clinical phenotypes. We have investigated 29 cases of GSDII and thereby identified 55 pathogenic mutations of the acid α-glucosidase gene (GAA) encoding acid maltase. There were 34 different mutations identified, 22 of which were novel. All of the missense mutations and two other mutations with an unpredictable effect on acid α-glucosidase synthesis and function were transiently expressed in COS cells. The effect of a novel splice-site mutation was investigated by real-time PCR analysis. The outcome of our analysis underscores the notion that the clinical phenotype of GSDII is largely dictated by the nature of the mutations in the GAA alleles. This genotype-phenotype correlation makes DNA analysis a valuable tool to help predict the clinical course of the disease.. ...
Recent studies have established the regulatory role of transcription factor EB (TFEB) in the lysosomal/autophagosomal fusion and exocytosis. We have shown that transcription factor E3 (TFE3) is another master regulator of lysosomal/autophagosomal biogenesis. ChIP-seq in muscle cells identified , 1000 TFE3 direct targets which are similar to known TFEB binding locations. The studies on the regulatory role of TFEB/TFE3 stem from our longstanding interest in Pompe disease, a lysosomal glycogen storage disorder caused by a deficiency of acid alpha glucosidase (GAA). In this severe myopathy, lysosomal glycogen accumulation leads to excessive autophagy and inhibition of the autophagic flux. Autophagic defect is associated with poor muscle response to enzyme replacement therapy. An alternative approach relies on the ability of TFEB and TFE3 to re-establish autophagic flux and induce lysosomal exocytosis in affected muscles. Indeed, overexpression of TFEB or TFE3 in Pompe muscle reduced the lysosomal ...
Infantile-onset Pompe disease is definitely an autosomal recessive disorder caused by the total loss of lysosomal glycogen-hydrolyzing enzyme acid -glucosidase (GAA) activity, which results in lysosomal glycogen build up and prominent cardiac and skeletal muscle pathology. higher electrophoretic mobility compared with control iPSC-CMs. Brefeldin A caused disruption of the Golgi in control iPSC-CMs reproduced the higher mobility forms of the LAMPs, suggesting that Pompe iPSC-CMs create LAMPs lacking appropriate glycosylation. Isoelectric focusing studies exposed that Light2 offers a more alkaline pI in Pompe compared with control iPSC-CMs due mainly to hyposialylation. MALDI-TOF-MS analysis of = 100 m for and … TABLE 2 iPS cell collection nomenclature, Paeoniflorin supplier GAA genotype, and phenotype Pompe iPS Cells Have Disease-causing Acid -Glucosidase Mutations Ensuing in Undetectable Mature Protein and Enzymatic Activity The mutations in the gene in the unique Pompe fibroblasts were ...
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TY - JOUR. T1 - Effects of the α-glucosidase inhibitor acarbose on postprandial serum glucose and insulin concentrations in healthy dogs. AU - Robertson, Jane. AU - Nelson, Richard W. AU - Kass, Philip H. AU - Neal, Larry. PY - 1999/5. Y1 - 1999/5. N2 - Objective - To determine effects of acarbose on baseline and postprandial serum glucose and insulin concentrations in healthy dogs, if effects of acarbose were dosage related, and if acarbose caused any short- term adverse effects. Animals - 5 healthy dogs fed a high-fiber diet. Procedure - A Latin-square design was used. During each 1-week treatment period, dogs were given a placebo or 25, 50, 100, or 200 mg of acarbose, PO, twice daily immediately prior to feeding. There was a 1-week interval between periods. At the end of each treatment period, serum glucose and insulin concentrations were measured prior to feeding and at 30- to 60-minute intervals for 6 hours after feeding. Results - Baseline serum glucose and insulin concentrations, insulin ...
Pompe disease is an autosomal recessive lysosomal storage disorder caused by disease‐associated variants in the acid alpha‐glucosidase (GAA) gene. The current Pompe mutation database provides a severity rating of GAA variants based on in silico predictions and expression studies. Here, we extended the database with clinical information of reported phenotypes. We added additional in silico predictions for effects on splicing and protein function and for cross reactive immunologic material (CRIM) status, minor allele frequencies, and molecular analyses. We analyzed 867 patients and 562 GAA variants. Based on their combination with a GAA null allele (i.e., complete deficiency of GAA enzyme activity), 49% of the 422 disease‐associated variants could be linked to classic infantile, childhood, or adult phenotypes. Predictions and immunoblot analyses identified 131 CRIM negative and 216 CRIM positive variants. While disease‐associated missense variants were found throughout the GAA protein, ...
Pompe disease is seen as a deficiency or absence of activity of the lysosomal enzyme acid alpha-glucosidase. was safely reintroduced during the IMT induction phase and, subsequently, the enzyme dose was increased, all without any complications. Antibodies disappeared, IMT was tapered and discontinued, and cadiomyopathy steadily improved. During 1 year of follow-up, she remained ventilator dependent and no gains in motor skills were noticed; motor features can end up being monitored during suffered ERT. Even though the reversal of medical decline inside our CRIM-positive and antibody-positive baby with Pompe disease cant be solely related to IMT, our encounters with this process may be beneficial to additional doctors encountering comparable therapeutic dilemmas. Intro Pompe disease (OMIM #232300), referred to as glycogen storage space disease type II also, can be a treatable lysosomal storage space disorder due to the current presence of a mutation in the gene encoding acidity alpha-glucosidase ...
TY - JOUR. T1 - Impaired organization and function of myofilaments in single muscle fibers from a mouse model of Pompe disease. AU - Xu, Sengen. AU - Galperin, Mikhail. AU - Melvin, Gary. AU - Horowits, Robert. AU - Raben, Nina. AU - Plotz, Paul. AU - Yu, Leepo. PY - 2010/5. Y1 - 2010/5. N2 - Pompe disease, a defi-ciency of lysosomal acid α-glucosidase, is a disorder of glycogen, metabolism that can affect infants, children, or adults. In all forms of the disease, there is progressive muscle pathology leading to premature death. The pathology is characterized by accumulation of glycogen in lysosomes, autophagic buildup, and muscle atrophy. The purpose of the present investigation was to determine if myofibrillar dysfunction in Pompe disease contributes to muscle weakness beyond that attributed to atrophy. The study was performed on isolated myofibers dissected from severely affected fast glycolytic muscle in the α-glucosidase knockout mouse model. Psoas muscle fibers were first permeabilized, ...
The research of medical geneticist Silvia Tortorelli, M.D., Ph.D., and her Mayo colleagues is outlined in, Moonlighting Newborn Screening Markers: The Incidental Discovery of a Second-Tier Test for Pompe Disease, which appeared as an advanced publication in Genetics in Medicine on November 2.. Through the teams work, a novel biochemical marker was discovered in dried blood spots that not only allows for a faster turnaround time of results but will be more cost-effective than molecular genetic analysis. The new marker was calculated by dividing the creatine/creatinine ratio by the activity of acid A-glucosidase.. According to Dr. Tortorelli, Consultant in the Biochemical Genetics Laboratory, the team used tools created by the Collaborative Laboratory Integrated Reports (CLIR) software to incorporate the new marker into an interpretation algorithm that achieved almost complete segregation between Pompe disease and false-positive cases.. This new test, once further clinically validated, will ...
1. The Ayurvedic Pharmacopoeia of India, Part-1, Vol-1 & 3.. 2. Yang ZD, Duan DZ, Xue WW, et al. Steroidal alkaloids from Holarrhena antidysenterica as acetylcholinesterase inhibitors and the investigation for structure-activity relationships. Life Sci. 2012 Jun 14;90(23-24):929-33.. 3. Kavitha D, Niranjali S. Inhibition of enteropathogenic Escherichia coli adhesion on host epithelial cells by Holarrhena antidysenterica (L.) WALL. Phytother Res. 2009 Sep;23(9):1229-36.. 4. Kavitha D, Shilpa PN, Devaraj SN. Antibacterial and antidiarrhoeal effects of alkaloids of Holarrhena antidysenterica WALL. Indian J Exp Biol. 2004 Jun;42(6):589-94.. 5. Ali KM, Chatterjee K, De D, et al. Inhibitory effect of hydro-methanolic extract of seed of Holarrhena antidysenterica on alpha-glucosidase activity and postprandial blood glucose level in normoglycemic rat. J Ethnopharmacol. 2011 Apr 26;135(1):194-6.. 6. Khan A, Khan SR, Gilani AH. Studies on the in vitro and in vivo antiurolithic activity of Holarrhena ...
Lumizyme (alglucosidase alfa) is an enzyme replacement treatment for Pompe disease developed by Sanofi Genzyme and approved by the FDA.
Hi everybody! My name is really Arthur, but some people call me Red. Hope you like the case format. These cases are not complete and are not intended to really teach, but are just for fun.....A three month old girl presents with profound muscle weakness, and Floppyness. On exam, hepatomegaly is noted. The chest X-ray is shown. Note Cardiomegaly. There has been no exposure to honey or home canned goods. .....The child has Pompe disease......An autosomal recessively inherited lysosomal storage disease. The deficient enzyme is alpha glucosidase, which is needed for glycogen breakdown.......The disease was first characterized by Johann Pompe in 1932. Pompe was danish, and was executed in 1945 by Nazi Germany for espionage.....The disease is also featured prominantly in the upcoming film Extraordinary Measures, which I believe is a statement against big pharama, and Bristol Myers Squib in particular. ......Besides Botulism (alluded to in the pertinent negatives of the case), a differential would ...
All Pompe disease patients have symptoms that affect their hearts, so its important to maintain healthy habits to ensure good cardiac health.
Evidence-Based Complementary and Alternative Medicine (eCAM) is an international peer-reviewed, Open Access journal that seeks to understand the sources and to encourage rigorous research in this new, yet ancient world of complementary and alternative medicine.
Evidence-Based Complementary and Alternative Medicine (eCAM) is an international peer-reviewed, Open Access journal that seeks to understand the sources and to encourage rigorous research in this new, yet ancient world of complementary and alternative medicine.
This trial is investigating the effects of miglitol [Sanwa Kagaku Kenkyusho] versus acarbose versus sitagliptin versus no treatment on glucose metabolism and
cell periphery, cytoplasm, extracellular region, fungal-type vacuole, mitochondrion, beta-fructofuranosidase activity, inulinase activity, sucrose alpha-glucosidase activity, fructan catabolic process, inulin catabolic process
Pompe disease is inherited in an autosomal recessive manner, meaning that an affected individual must inherit an abnormal allele from both parents. Thus, t
Research Report on Global Pompe Disease Drugs Sales Market Report 2017. The Report includes market price, demand, trends, size, Share, Growth, Forecast, Analysis & Overview.
Complete information for GAA gene (Protein Coding), Glucosidase Alpha, Acid, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
N-Glycans are modified as part of a quality control mechanism during glycoprotein folding in the endoplasmic reticulum (ER). Glucosidase II (GII) plays a critical role by generating monoglucosylated glycans that are recognized by lectin chaperones, calnexin and calreticulin. To understand how the hydrolytic activity of GIIα is enhanced by the mannose 6-phosphate receptor (MPR) homology domain (MRH domain) of its β subunit, we now report a 1.6 Å resolution crystal structure of the MRH domain of GIIβ bound to mannose. A comparison of ligand-bound and unbound structures reveals no major difference in their overall fold, but rather a repositioning of side chains throughout the binding pocket, including Y372. Mutation of Y372 inhibits GII activity, demonstrating an important role for Y372 in regulating GII activity. Comparison of the MRH domains of GIIβ, MPRs, and the ER lectin OS-9 identified conserved residues that are critical for the structural integrity and architecture of the carbohydrate ...
Acarbose, alpha-glucosidase inhibitor (CAS 56180-94-0), with |99% purity. Water soluble compound. Join researchers using our high quality biochemicals.
"GAA: glucosidase alpha, acid". NIH Genetics Home Reference. US Government.. ...
... alpha-glucosidase (EC 3.2.1.20), alpha-galactosidase (EC 3.2.1.22); glucoamylase (EC 3.2.1.3), sucrase-isomaltase (EC 3.2.1.48 ... Hermans MM, Kroos MA, van Beeumen J, Oostra BA, Reuser AJ (July 1991). "Human lysosomal alpha-glucosidase. Characterization of ... Homology with the rabbit intestinal sucrase-isomaltase complex and human lysosomal alpha-glucosidase". European Journal of ... and lysosomal alpha-glucosidase. GH31 in CAZypedia Henrissat B, Callebaut I, Fabrega S, Lehn P, Mornon JP, Davies G (July 1995 ...
Kamimura H, Ogata H, Takahara H (1992). "Alpha-glucoside formation of xenobiotics by rat liver alpha-glucosidases". Drug ... Neutral alpha-glucosidase C is an enzyme that in humans is encoded by the GANC gene. Glycoside hydrolase enzymes hydrolyse the ... "Entrez Gene: GANC glucosidase, alpha; neutral C". Feizi T, Larkin M (Sep 1990). "AIDS and glycosylation". Glycobiology. 1 (1): ... Martiniuk F, Hirschhorn R, Smith M (Oct 1980). "Assignment of the gene for human neutral alpha-glucosidase C to chromosome 15 ...
... alpha-glucosidase (EC 3.2.1.20); glucodextranase (EC 3.2.1.70). Glucoamylase (GA) catalyses the release of D-glucose from the ... The protein belongs to the mainly alpha class, and contains 19 helices and 9 strands. Henrissat B, Callebaut I, Fabrega S, Lehn ...
"Entrez Gene: maltase-glucoamylase (alpha-glucosidase)". Nichols BL, Eldering J, Avery S, Hahn D, Quaroni A, Sterchi E (January ... Alpha-glucosidase Maltase ENSG00000282607 GRCh38: Ensembl release 89: ENSG00000257335, ENSG00000282607 - Ensembl, May 2017 ... Maltase-glucoamylase is an alpha-glucosidase digestive enzyme. It consists of two subunits with differing substrate specificity ... Frandsen TP, Svensson B (May 1998). "Plant alpha-glucosidases of the glycoside hydrolase family 31. Molecular properties, ...
Glucan 1,4-alpha-glucosidase. Identifiers. EC number. 3.2.1.3. CAS number. 9032-08-0 ... γ-Amylase (EC 3.2.1.3 ) (alternative names: Glucan 1,4-a-glucosidase; amyloglucosidase; exo-1,4-α-glucosidase; glucoamylase; ... Main article: Alpha-amylase. The α-amylases (EC 3.2.1.1 ) (CAS 9014-71-5) (alternative names: 1,4-α-D-glucan glucanohydrolase; ... An inhibitor of alpha-amylase, called phaseolamin, has been tested as a potential diet aid.[10] ...
6-glucosidase (EC 3.2.1.33), or glucosidase, cleaves the remaining alpha-1,6 linkage, producing glucose and a linear chain of ... Yamamoto E, Makino Y, Omichi K (May 2007). "Active site mapping of amylo-alpha-1,6-glucosidase in porcine liver glycogen ... Gillard BK, White RC, Zingaro RA, Nelson TE (September 1980). "Amylo-1,6-glucosidase/4-alpha-glucanotransferase. Reaction of ... Gillard BK, Nelson TE (September 1977). "Amylo-1,6-glucosidase/4-alpha-glucanotransferase: use of reversible substrate model ...
... acid alpha-glucosidase, MGAM, TFF1, TFF2, TFF3, and ZP4. SCOP alpha/beta knot fold CATH alpha/beta knot topology pKNOT the web ... and lysosomal alpha-glucosidase (EC 3.2.1.20). Human gene encoding proteins containing the trefoil domain include: ... The yeast ribosome synthesis factor Emg1 is a novel member of the superfamily of alpha/beta knot fold methyltransferases. ...
6-phospho-alpha-glucosidase (EC 3.2.1.122); alpha-galactosidase (EC 3.2.1.22). 6-phospho-alpha-glucosidase requires both NAD(H ... dependent 6-phospho-alpha-glucosidase. Assignment to family 4 of the glycosylhydrolase superfamily". The Journal of Biological ... Glycoside hydrolase family 4 CAZY GH_4 comprises enzymes with several known activities; 6-phospho-beta-glucosidase (EC 3.2.1.86 ...
The extract also had alpha-glucosidase inhibitory activity. Yam phak khut, a Thai salad of fern leaves and pork Ensaladang pako ... 28 (2). Chai TT, Yeoh LY, Mohd Ismail NI, Ong HC, Abd Manan F, Wong FC (2015) Evaluation of glucosidase inhibitory and ...
Glucosidase I is the first enzyme in the N-linked oligosaccharide processing pathway. GCS1 cleaves the distal alpha-1,2-linked ... Ratner L, vander Heyden N, Dedera D (1991). "Inhibition of HIV and SIV infectivity by blockade of alpha-glucosidase activity". ... Kalz-Fuller B, Heidrich-Kaul C, Nothen M, Bause E, Schwanitz G (Sep 1996). "Localization of the human glucosidase I gene to ... "Entrez Gene: GCS1 glucosidase I". Goodman CD, McFadden GI (2008). "Gamete Fusion: Key Protein Identified". Current Biology. 18 ...
Alpha-glucosidase inhibitor Miglustat Voglibose "Migliotl: MedlinePlus Drug Information". MedlinePlus. National Institutes of ... In contrast to acarbose (another alpha-glucosidase inhibitor), miglitol is systemically absorbed; however, it is not ... Miglitol, and other structurally-related iminosugars, inhibit glycoside hydrolase enzymes called alpha-glucosidases. Since ...
7] GANAB glucosidase II alpha subunit Homo sapiens (human). Gene ID:23193, 2016. Chen J, Deng F, Singh SV, Wang QJ (May 2008 ... The GANAB gene codes the catalytic alpha subunit of glucosidase II and noncatalytic beta subunit; the glycosyl hydrolase 31 ... The heterodimeric glucosidase II enzyme has an important function in protein folding and catalyzes the hydrolysis of glucose ... Yuan J, Rey O, Rozengurt E (2006). "Activation of protein kinase D3 by signaling through Rac and the alpha subunits of the ...
Drug Concentration Monitoring Microbial Alpha-Glucosidase Inhibitors Plasminogen Activators. Berlin, Heidelberg: Springer ...
It is a dual-function enzyme with two GH31 domains, one serving as the isomaltase, the other as a sucrose alpha-glucosidase. It ... "SI sucrase-isomaltase (alpha-glucosidase) [Homo sapiens (human)] - Gene - NCBI". Brunner, J.; Hauser, H.; Braun, H.; Wilson, K ... Sucrase-isomaltase (SI) is a glucosidase enzyme located on the brush border of the small intestine. ...
... but levels of alpha-glucosidase determines the type of GSD II an individual may have. More alpha glucosidase present in the ... The disease is caused by a mutation in a gene (acid alpha-glucosidase: also known as acid maltase) on long arm of chromosome 17 ... The gene encodes a protein-acid alpha-glucosidase (EC 3.2.1.20)-which is a lysosomal hydrolase. The protein is an enzyme that ... It is caused by an accumulation of glycogen in the lysosome due to deficiency of the lysosomal acid alpha-glucosidase enzyme. ...
Ullah, N.; Haladu, S. A. (2010). "The first total synthesis of aspergillusol A, an alpha-glucosidase inhibitor". Natural ... Aspergillusol A is an alpha-glucosidase inhibitor isolated from marine Aspergillus. Structurally, it consists of an erythritol ... an alpha-glucosidase inhibitor from the marine-derived fungus Aspergillus aculeatus". Journal of Natural Products. 72 (11): ...
Durantel, D. (2009). "Celgosivir, an alpha-glucosidase I inhibitor for the potential treatment of HCV infection". Current ... "Castanospermine inhibits alpha-glucosidase activities and alters glycogen distribution in animals". PNAS. 82 (1): 93-97. doi: ... It is a potent inhibitor of some glucosidase enzymes and has antiviral activity in vitro and in mouse models. The ...
... s were also found to present alpha-glucosidase inhibitory activity through artificial organic synthesis. ... Synthesis of novel flavonoid alkaloids as α-glucosidase inhibitors. Bioorganic & Medicinal Chemistry. Jain SK, Bharate SB, ...
Bioactive molecules extracted from the leaves have been reported to have alpha-glucosidase inhibitor activity. Botanic Gardens ... A Potential α-Glucosidase Inhibitor". BioMed Research International. 2013: 1-6. doi:10.1155/2013/591313. ISSN 2314-6133. PMC ...
"Identification of the CD45-associated 116-kDa and 80-kDa proteins as the alpha- and beta-subunits of alpha-glucosidase II". J. ... "Identity of neutral alpha-glucosidase AB and the glycoprotein processing enzyme glucosidase II. Biochemical and genetic studies ... Neutral alpha-glucosidase AB is an enzyme that in humans is encoded by the GANAB gene. GANAB has been shown to interact with ... Ratner L, vander Heyden N, Dedera D (1991). "Inhibition of HIV and SIV infectivity by blockade of alpha-glucosidase activity". ...
Alpha glucosidase inhibitors delay glucose absorption at the intestine level and thereby prevent sudden surge of glucose after ... Voglibose (INN and USAN, trade name Voglib, marketed by Mascot Health Series) is an alpha-glucosidase inhibitor used for ... Chen X, Zheng Y, Shen Y (2006). "Voglibose (Basen, AO-128), one of the most important alpha-glucosidase inhibitors". Current ...
... alpha-D-glucosidase, alpha-glucoside hydrolase, alpha-1,4-glucosidase, alpha-D-glucoside glucohydrolase) is one type of alpha- ... Maltase (EC 3.2.1.20, alpha-glucosidase, glucoinvertase, glucosidosucrase, maltase-glucoamylase, alpha-glucopyranosidase, ... Alpha-amylase 1 which is coded on the AMY1A gene is responsible of cleaving α-glucosidase linkages in oligosaccharides and ... The following are genes that can code for maltase: Acid alpha-glucosidase which is coded on the GAA gene is essential to ...
The aqueous extracts of the gall also inhibit alpha-glucosidase activity in vitro. foliage flowers fruits sapling "Rhus ... "Inhibitory effect of aqueous extract from the gall of Rhus chinensis on alpha-glucosidase activity and postprandial blood ...
January 2007). "Recombinant human acid [alpha]-glucosidase: major clinical benefits in infantile-onset Pompe disease". ... alpha-glucosidase. It is the first drug available to treat this disease. It was approved for medical use in the United States ...
Durantel D (August 2009). "Celgosivir, an alpha-glucosidase I inhibitor for the potential treatment of HCV infection". Current ... is an oral prodrug of the natural product castanospermine that inhibits alpha-glucosidase I, an enzyme that plays a critical ...
... alpha-glucosidase enzymes in the brush border of the small intestines, and pancreatic alpha-amylase. Pancreatic alpha-amylase ... It is a starch blocker, and inhibits alpha glucosidase, an intestinal enzyme that releases glucose from larger carbohydrates. ... Hedrington MS, Davis SN (2019). "Considerations when using alpha-glucosidase inhibitors in the treatment of type 2 diabetes". ... whereas the membrane-bound intestinal alpha-glucosidases hydrolyze oligosaccharides, trisaccharides, and disaccharides to ...
Kuo WL, Hirschhorn R, Huie ML, Hirschhorn K (1996). "Localization and ordering of acid alpha-glucosidase (GAA) and thymidine ...
Alpha-glucosidase activity is elevated in the posterior midgut after feeding in response to the blood meal, whereas activity in ... Alpha-glucosidase is active in anterior and posterior midguts before and at all times after feeding. In whole midgut ... Depending on the time after feeding, greater than 25% of the total midgut activity of alpha-glucosidase is located in the ... and alpha-glucosidase in the midgut". Journal of Medical Entomology. 28 (6): 865-71. doi:10.1093/jmedent/28.6.865. PMID 1770523 ...
It is a multienzyme complex which possesses alpha-L-rhamnosidase and beta glucosidase active centers. The E.C. No.(EC 3.2.1.40 ... Lastly glucosidase breaks prunin into glucose and naringenin, a flavorless flavanone also found in various citrus. Ram gene is ...
Alpha-glucosidase. *3.4 Protease: Exopeptidase *DPP-4. *ACE. *Endopeptidase *Trypsin. *Renin. *Mixed *Enkephalinase ...
... α1-6 glucosidase enzyme is required to break the remaining (single glucose) α1-6 residue that remains in the new linear chain. ... catalyzes the rate-limiting step in glycogenolysis in animals by releasing glucose-1-phosphate from the terminal alpha-1,4- ...
alpha-1,4-glucosidase activity. • alpha-glucosidase activity. Cellular component. • membrane. • lysosomal membrane. • lysosomal ... Acid alpha-glucosidase, also called α-1,4-glucosidase[5] and acid maltase,[6] is an enzyme (EC 3.2.1.20) that helps to break ... maltose alpha-glucosidase activity. • catalytic activity. • hydrolase activity. • carbohydrate binding. • isomaltase. • ... This gene encodes lysosomal alpha-glucosidase, which is essential for the degradation of glycogen to glucose in lysosomes. ...
... beta-D-glucosidase, beta-glucoside glucohydrolase, arbutinase, amygdalinase, p-nitrophenyl beta-glucosidase, primeverosidase, ... Vicianin beta-glucosidase. References[edit]. *^ PDB: 3AHX​; Jeng WY, Wang NC, Lin MH, Lin CT, Liaw YC, Chang WJ, Liu CI, Liang ... Synonyms, derivatives, and related enzymes include gentiobiase, cellobiase, emulsin,[3] elaterase, aryl-beta-glucosidase, ... Beta-glucosidase catalyzes the hydrolysis of the glycosidic bonds to terminal non-reducing residues in beta-D-glucosides and ...
Separation and characterization of cellulases and beta-glucosidases". The Biochemical Journal. 177 (1): 9-19. doi:10.1042/ ... McCleary BV (November 1980). "New chromogenic substrates for the assay of alpha-amylase and (1 leads to 4)-beta-D-glucanase". ... Colourimetric and fluorimetric cellulase substrates can be used in the presence of ancillary β-glucosidase for the specific ... The ancillary enzyme present in the reagent mixture (β-glucosidase) then acts to hydrolyse the fragment containing the ...
Alpha-glucosidase inhibitors. *Acarbose. *Miglitol. *Voglibose. Amylin analog. *Pramlintide. Sodium glucose transporter (SGLT2 ...
Ding, F; JM Borreguero; SV Buldyrey; HE Stanley; NV Dokholyan (2003). "Mechanism for the alpha-helix to beta-hairpin transition ... "Comparison of a molecular dynamics model with the X-ray structure of the N370S acid-beta-glucosidase mutant that causes Gaucher ... Smith, A; CK Hall (2001). "Alpha-Helix Formation: Discontinuous Molecular Dynamics on an Intermediate-Resolution Protein Model ...
EC 3.2.1.48 is sucrose alpha-glucosidase. Physiology[edit]. Sucrose intolerance (also known as congenital sucrase-isomaltase ...
For example, a combination of alpha-amylase and cellulase was shown to degrade polymicrobial bacterial biofilms from both in ... Thus glucosidases catalyze the hydrolysis of glucosides and xylanases catalyze the cleavage of the xylose based homopolymer ...
Alpha-glucosidase activity is elevated in the posterior midgut after feeding in response to the blood meal, whereas activity in ... Alpha-glucosidase is active in anterior and posterior midguts before and at all times after feeding. In whole midgut ... Depending on the time after feeding, greater than 25% of the total midgut activity of alpha-glucosidase is located in the ... and alpha-glucosidase in the midgut"។ Journal of Medical Entomology 28 (6): 865-871។ 1991។ អ.ស.ផ.ម. 1770523។ ...
Alpha-glucosidase. *3.4 Protease: Exopeptidase *DPP-4. *ACE. *Endopeptidase *Trypsin. *Renin. *Mixed *Enkephalinase ...
... a lineage of iso-maltose glucosidases and a lineage that further split into maltose glucosidases and iso-maltose glucosidases. ... Maier TH (April 2003). "Semisynthetic production of unnatural L-alpha-amino acids by metabolic engineering of the cysteine- ... In light of the large number of paralogous fungal α-glucosidase genes with a number of specific maltose-like (maltose, turanose ... Antithetically, the ancestor before the latter split had a more pronounced isomaltose-like glucosidase activity.[4] ...
Lee JY, Cho KS (May 2018). "Effects of 5-alpha reductase inhibitors: new insights on benefits and harms". Curr Opin Urol. 28 (3 ... Trost L, Saitz TR, Hellstrom WJ (2013). "Side Effects of 5-Alpha Reductase Inhibitors: A Comprehensive Review". Sex Med Rev. 1 ... "FDA Alert: 5-alpha reductase inhibitors (5-ARIs): Label Change - Increased Risk of Prostate Cancer". Drugs.com. Retrieved 2014- ... Wang LG, Mencher SK, McCarron JP, Ferrari AC (2004). "The biological basis for the use of an anti-androgen and a 5-alpha- ...
... and tumor necrosis factor-alpha in rats with sucrose-induced metabolic syndrome". J. Nutr. Biochem. 15 (6): 350-57. doi:10.1016 ...
Dworkin, Martin; Falkow, Stanley (2006). The Prokaryotes: Proteobacteria: alpha and beta subclasses. Springer. p. 169. ISBN ... β-glucosidase, β-xylosidase, or polygalacturonase, which Saccharomyces yeast may not produce.[68] ...
Matsuur H, Asakawa C, Kurimoto M, Mizutani J (July 2002). "Alpha-glucosidase inhibitor from the seeds of balsam pear (Momordica ... It lowers blood sugar because the mushroom naturally contains an alpha glucosidase inhibitor.[12] ...
Trost L, Saitz TR, Hellstrom WJ (2013). "Side Effects of 5-Alpha Reductase Inhibitors: A Comprehensive Review". Sex Med Rev. 1 ... "FDA Alert: 5-alpha reductase inhibitors (5-ARIs): Label Change - Increased Risk of Prostate Cancer". Drugs.com. Retrieved 2014- ... Wang LG, Mencher SK, McCarron JP, Ferrari AC (2004). "The biological basis for the use of an anti-androgen and a 5-alpha- ... Gur, S; Kadowitz, PJ; Hellstrom, WJ (January 2013). "Effects of 5-alpha reductase inhibitors on erectile function, sexual ...
Chiba S (august 1997). "Molecular mechanism in alpha-glucosidase and glucoamylase". Biosci. Biotechnol. Biochem. 61 (8): 1233-9 ... Yamamoto E, Makino Y, Omichi K (maj 2007). "Active site mapping of amylo-alpha-1,6-glucosidase in porcine liver glycogen ... Gillard BK, White RC, Zingaro RA, Nelson TE (septembar 1980). "Amylo-1,6-glucosidase/4-alpha-glucanotransferase. Reaction of ... Gillard BK, Nelson TE (septembar 1977). "Amylo-1,6-glucosidase/4-alpha-glucanotransferase: use of reversible substrate model ...
দেখা যায় যে, গ্লুকোজ সরাসরি রক্তে নিলে যতটা না ইনসুলিন তৈরি হয়, মুখে খেলে তার থেকে তিন গুণ বেশি তৈরি হয়। এর জন্য দায়ী দুটি হরমোন। এদের সংক্ষেপে ইনক্রেটিন বলা হয়। টাইপ ২ ডায়াবেটিসে এই ইনক্রেটিনের পরিমাণ কমে যায়। এক্ষেত্রে ইনক্রেটিনকে ভেঙ্গে ফেলে ডাইপেপটিডিল পেপটাইডেজ ৪ (DPP-4)। সিটাগ্লিপটিন এই DPP-4 কে বাধা দেয়। এটি এর ইনহিবিটর। এটি এর জন্য অনন্য (Selective) ইনহিবিটর। সর্বোপরি, ...
... while lysosomal alpha-glucosidase is responsible for carbohydrates, and lysosomal acid phosphatase is necessary to release ...
"Information on EC 3.2.1.20 - alpha-glucosidase). 16.10.16. *↑ William M. Fogarty, Catherine T. Kelly. 1990. Microbial Enzymes ... Bischoff, H. (1995). The mechanism of alpha-glucosidase inhibition in the management of diabetes. Clin Invest Med. 18(4): 303- ... 14,0 14,1 Kim, J.S., Kwon, C.S., Son, K.H. (2000). Inhibition of Alpha-glukosidase and Amylase by Luteonin, a Flavonoid. Biosci ... Park, J.E., Park, S.H., Woo, J.Y., Hwang, H.S., Cha, J., Lee, H. (2013). Enzymatic Properties of a Thermostable α-Glucosidase ...
Since acarbose and other alpha-glucosidase inhibitors prevent starch and other sugars from being broken down into ... Some forms of this respond to treatment with an alpha-glucosidase inhibitor, which slows starch digestion. ...
Glucosidases. *Cellulase. *Alpha-glucosidase *Acid. *Neutral AB. *Neutral C. *Beta-glucosidase *cytosolic ...
... cholesterol 7-alpha-monooxygenase activity, cholesterol oxidase activity, cholesterol 24-hydroxylase activity, 7- ... dehydrocholesterol reductase activity, steryl-beta-glucosidase activity, cholesterol allpha-glucosyltransferase activity Yn ...
Alpha-glucosidase inhibitors. *Acarbose. *Miglitol. *Voglibose. Amylin analog. *Pramlintide. Sodium glucose transporter (SGLT2 ...
displaystyle V={\frac {V_{max}[S]}{\alpha K_{m}+\alpha ^{\prime }[S]}}={\frac {(1/\alpha ^{\prime })V_{max}[S]}{(\alpha /\alpha ... displaystyle \alpha =1+{\frac {[I]}{K_{i}}}}. α. ′. =. 1. +. [. I. ]. K. i. ′. .. {\displaystyle \alpha ^{\prime }=1+{\frac {[I ... An example of a toxic peptide is alpha-amanitin, which is found in relatives of the death cap mushroom. This is a potent enzyme ... "Mechanism of the irreversible inactivation of mouse ornithine decarboxylase by alpha-difluoromethylornithine. Characterization ...
GBA: glucosidase, beta; acid (includes glucosylceramidase) (gene for Gaucher disease). *GBAP1: glucosylceramidase beta ... MAN1A2: Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB. *MEAF6: MYST/ESA1 associated factor 6 ...
GBA: glucosidase, beta; acid (includes glucosylceramidase) (gene para sa sakit na Gaucher) ... COL11A1: collagen, type XI, alpha 1. *CPT2: carnitine palmitoyltransferase II. *DBT: dihydrolipoamide branched chain ...
Alpha catenin. *Alpha-D-phosphohexomutase superfamily. *Alpha-Mannosidase. *Alpha-N-acetylglucosaminidase. *Alphavirus ... Beta-glucosidase. *Beta-ketoacyl-ACP synthase. *Beta-secretase. *Betaine-homocysteine S-methyltransferase ...
... may refer to: Glucan 1,4-a-glucosidase, an enzyme Acid alpha-glucosidase, an enzyme This set index ...
alpha-1,4-glucosidase activity. • alpha-glucosidase activity. Cellular component. • membrane. • lysosomal membrane. • lysosomal ... Acid alpha-glucosidase, also called α-1,4-glucosidase[5] and acid maltase,[6] is an enzyme (EC 3.2.1.20) that helps to break ... maltose alpha-glucosidase activity. • catalytic activity. • hydrolase activity. • carbohydrate binding. • isomaltase. • ... This gene encodes lysosomal alpha-glucosidase, which is essential for the degradation of glycogen to glucose in lysosomes. ...
4-linked alpha-D-glucose residue from the nonreducing end of a long chain (or polymer) of such residues, making an al... ... An enzyme which removes the last 1,4-linked alpha-D-glucose residue from the nonreducing end of a long chain (or polymer) of ... such residues, making an alpha-D-glucose molecule out of it in the process. ...
Alpha-glucosidase inhibitors (such as acarbose) decrease the absorption of carbohydrates from the digestive tract, thereby ... Alpha-glucosidase inhibitors (such as acarbose) decrease the absorption of carbohydrates from the digestive tract, thereby ...
... alpha-D-glucosidase, alpha-glucoside hydrolase, alpha-1,4-glucosidase, alpha-D-glucoside glucohydrolase) is a glucosidase ... Alglucosidase alfa Alpha-glucosidase inhibitor alpha-Glucosidases at the US National Library of Medicine Medical Subject ... Diabetes: Acarbose, an alpha-glucosidase inhibitor, competitively and reversibly inhibits alpha-glucosidase in the intestines. ... Other glucosidases include: Cellulase Beta-glucosidase Debranching enzyme Alpha-glucosidase hydrolyzes terminal non-reducing (1 ...
... alpha-1,4), sucrose (alpha-1,2), isomaltose (alpha-1,6) and turanose (alpha-1,3). ... Hydrolyzes a broad range of alpha-D-linked glucopyranosides, including maltose ( ... alpha-1,4), sucrose (alpha-1,2), isomaltose (alpha-1,6) and turanose (alpha-1,3). ... Alpha-glucosidase subunit 2Add BLAST. 458. Amino acid modifications. Feature key. Position(s). DescriptionActions. Graphical ...
Alpha-glucosidase Inhibitors are great treatment options for those with high glucose levels. Get facts about Alpha-glucosidase ... Possible Side Effects of Alpha-Glucosidase Inhibitors. You may have these side effects when you take an alpha-glucosidase ... Possible Drug Interactions With Alpha-Glucosidase Inhibitors. Before you take an alpha-glucosidase inhibitor, tell all your ... People who have a history of stomach or bowel trouble should not use alpha-glucosidase inhibitors. Not everyone who takes alpha ...
Glucan endo-1,3-alpha-glucosidase (EC 3.2.1.59, endo-1,3-alpha-glucanase, mutanase, endo-(1->3)-alpha-glucanase, cariogenase, ... Glucan+endo-1,3-alpha-glucosidase at the US National Library of Medicine Medical Subject Headings (MeSH) Biology portal. ... 3-alpha-D-glucan) are nigerose and alpha-D-glucose. Hasegawa S, Nordin JH (October 1969). "Enzymes that hydrolyze fungal cell ... alpha-D-glucan 3-glucanohydrolase) is an enzyme with systematic name 3-alpha-D-glucan 3-glucanohydrolase. The enzyme catalyses ...
Lysosomal alpha-glucosidase may refer to: Glucan 1,4-a-glucosidase, an enzyme Acid alpha-glucosidase, an enzyme This set index ...
Glucosidase 2 subunit betaNeutral alpha-glucosidase AB1,2-ETHANEDIOL5-FLUORO-BETA-D-GLUCOPYRANOSEALPHA-D-MANNOSECALCIUM IOND ...
alpha Glucosidases. Enzymes that catalyze the exohydrolysis of 1,4-alpha-glucosidic linkages with release of alpha-Glucose. ... Deficiency of alpha-1,4-Glucosidase may cause Glycogen Storage Disease Type II. ...
The alpha-glucosidase inhibitors available today (with brand names in parenthesis) are: acarbose (Precose) miglitol (Glyset) An ... Alpha-glucosidase inhibitors (AGIs) are a class of drugs used to treat type 2 diabetes. The key benefit of AGIs is that they do ... Alpha-Glucosidase Inhibitors. Alpha-glucosidase inhibitors (AGIs) are a class of drugs used to treat type 2 diabetes. The key ...
F. Ye, Z. Shen, and M. Xie, "Alpha-glucosidase inhibition from a Chinese medical herb (Ramulus mori) in normal and diabetic ... Inhibitory Properties of Aqueous Ethanol Extracts of Propolis on Alpha-Glucosidase. Hongcheng Zhang,1,2 Guangxin Wang,1 Trust ... L. J. Shai, P. Masoko, M. P. Mokgotho et al., "Yeast alpha glucosidase inhibitory and antioxidant activities of six medicinal ... "Effective control of postprandial glucose level through inhibition of intestinal alpha glucosidase by Cymbopogon martinii (Roxb ...
Inhibitory Activity Assay for Bakers Yeast Alpha-Glucosidase. Alpha-glucosidase inhibitory effect of propolis extracts was ... glucosidase.. In the present study, we assessed inhibitory effects of aqueous ethanol extracts of propolis on alpha-glucosidase ... glucosidase was tested according to procedure described previously [4] with minor modifications. Alpha-glucosidase activity was ... Inhibition of Aqueous Ethanol Extracts of Propolis against Alpha-Glucosidase. The -glucosidase inhibitory activity of various ...
Feb 14, 2014 Alpha-glucosidase Therapy Center, DPP-4 Therapy Center, Featured Writers, GLP-1 Agonist Therapy Center, Surgery ... Aug 23, 2012 Alpha-glucosidase Therapy Center, Alzheimers, Articles Scientists have found compounds that inhibit enzymes ... Apr 19, 2012 Alpha-glucosidase Therapy Center, Articles, GLP-1 Agonist Therapy Center ... May 9, 2013 Alpha-glucosidase Therapy Center, Articles, SGLT-2 Therapy Center ...
Rabbit polyclonal alpha Glucosidase II antibody validated for Western Blot, IHC, ICC/IF & tested in Human & Mouse. Immunogen ... Anti-alpha Glucosidase II antibody. See all alpha Glucosidase II primary antibodies. ... Anti-alpha Glucosidase II antibody (ab96757) at 1/1000 dilution + NIH-3T3 whole cell lysate at 30 µg. Predicted band size: 107 ... All lanes : Anti-alpha Glucosidase II antibody (ab96757) at 1/1000 dilution. Lane 1 : 293T whole cell lysate. Lane 2 : A431 ...
Recombinant human acid alpha-glucosidase is safe and effective for treatment of infantile-onset Pompe disease. Eleven patients ... Recombinant human acid [alpha]-glucosidase: major clinical benefits in infantile-onset Pompe disease Neurology. 2007 Jan 9;68(2 ... Conclusions: Recombinant human acid alpha-glucosidase is safe and effective for treatment of infantile-onset Pompe disease. ... Pompe disease is a progressive metabolic neuromuscular disorder resulting from deficiency of lysosomal acid alpha-glucosidase ( ...
The use of alpha-glucosidase inhibitors results in a lower, delayed rise in blood glucose after a meal when taken with the ... Alpha-Glucosidase Inhibitors. By Mark Marino , Published September 4, 2009. , Updated December 22, 2014. ... The use of alpha-glucosidase inhibitors results in a lower, delayed rise in blood glucose after a meal. Since these drugs lower ... Alpha-glucosidase is an intestinal enzyme that breaks down a variety of carbohydrates, but two of the more important ones are ...
Browse our alpha Glucosidase 2 Lysate catalog backed by our Guarantee+. ... Alternate Names for alpha Glucosidase 2 Lysates. alpha Glucosidase 2 lysate, GANAB lysate, Alpha-glucosidase 2 lysate, EC 3.2.1 ... KIAA0088alpha glucosidase II alpha subunit lysate, neutral alpha-glucosidase AB lysate. 2 Results for "alpha-glucosidase-2" in ... We offer alpha Glucosidase 2 Lysates for use in common research applications: Western Blot. Each alpha Glucosidase 2 Lysate is ...
Alpha-glucosidase inhibitors, sometimes referred to as starch blockers, are anti-diabetic medicines that help to reduce post ... Who are alpha-glucosidase inhibitors suitable for?. Alpha-glucosidase inhibitors are suitable for people with type 2 diabetes ... Alpha-glucosidase inhibitors slow down the digestion of carbohydrates Alpha-glucosidase inhibitors (AGIs), sometimes referred ...
Alpha 1,6-glucosidaseImported. ,p>Information which has been imported from another database using automatic procedures.,/p> ,p ... tr,E1AFY9,E1AFY9_YEASX Alpha 1,6-glucosidase OS=Saccharomyces cerevisiae GN=IMA4 PE=4 SV=1 ...
... alpha-glucosidase inhibitors may not be a good option for you. It is not known i ... If you are pregnant, planning to become pregnant, or may be pregnant, alpha-glucosidase inhibitors may not be a good option for ... If you have inflammatory bowel disease, you should not take an alpha-glucosidase inhibitor. ... If you have kidney impairment, you should not take an alpha-glucosidase inhibitor. ...
Alpha-glucosidase inhibitors, a class of drugs also known as starch blockers, function by slowing the absorption of certain ... A second alpha-glucosidase inhibitor, miglitol (Glyset) became available in 1999. They may be used by people with either Type 1 ... However, while alpha-glucosidase inhibitors themselves do not cause low blood glucose, when they are used in combination with ... The first alpha-glucosidase inhibitor to become available in the United States was acarbose (Precose), which was approved by ...
The GAA gene encodes the lysosomal enzyme alpha-1,4-glucosidase, which is essential for the degradation of glycogen to glucose ... Loss of function mutations lead to acid alpha-glucosidase deficiency thus glycogen accumulates in lysosomes and cytoplasm. This ...
Compare and order alpha-Glucosidase ELISA Kits. View citations, images, detection ranges, sensitivity, prices and more. ... glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) , lysosomal alpha-glucosidase , CG11909-PA , alpha- ... Images for product: alpha-Glucosidase (AGLU) ELISA Kit Diagramm of the ELISA kit to detect Rat a-Gluwith the optical density on ... Images for product: alpha-Glucosidase (AGLU) ELISA Kit Diagramm of the ELISA kit to detect Rat a-Gluwith the optical density on ...
Compare and order alpha-Glucosidase ELISA Kits. View citations, images, detection ranges, sensitivity, prices and more. ... alpha glucosidase 2 , maltase , alpha-glucosidase , CG11909-PA , alpha-Glucosidase , tobi-PA , acid (Pompe disease, glycogen ... acid alpha-glucosidase , acid maltase , glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) , lysosomal ... Search alpha-Glucosidase ELISA Kits for other reactivities: Mammalian,. Rabbit,. Guinea Pig,. Pig (Porcine),. Dog (Canine),. ...
We examined the inhibitory and binding effects of four imino sugars on a recombinant human acid alpha-glucosidase, ... Binding parameters and thermodynamics of the interaction of imino sugars with a recombinant human acid alpha-glucosidase ( ... which act as potential inhibitors of acid alpha-glucosidases in vitro, to improve the stability and/or transportation of mutant ... acid alpha-glucosidases in cells was studied and attracted interest. However, the mechanism underlying the molecular ...
Safety Study of Recombinant Adeno-Associated Virus Acid Alpha-Glucosidase to Treat Pompe Disease. The safety and scientific ... Have a diagnosis of Pompe, as defined by protein assay, DNA sequence of the acid alpha-glucosidase gene and clinical symptoms ... Phase I/II Trial of Diaphragm Delivery of Recombinant Adeno-Associated Virus Acid Alpha-Glucosidase (rAAV1-CMV-GAA) Gene Vector ... Phase I/II trial of adeno-associated virus-mediated alpha-glucosidase gene therapy to the diaphragm for chronic respiratory ...
walsbyi MalH was most similar (up to 41%) to alpha-glucosidases and alpha-xylosidases of Halorubrum. Moreover, computational ... To our knowledge, this is the first report on the characterization of an alpha-glucosidase from the halophilic Archaea, which ... Phylogenetic analyses, with respect to other alpha-glucosidases from members of the class Halobacteria, showed that the Hqr. ... coli YicI-like alpha-xylosidase of the GH31 family. However, the purified enzyme did not show alpha-xylosidase activity. This ...
What is oligo-alpha-1,6-glucosidase? Meaning of oligo-alpha-1,6-glucosidase medical term. What does oligo-alpha-1,6-glucosidase ... 6-glucosidase in the Medical Dictionary? oligo-alpha-1,6-glucosidase explanation free. ... Oligo-alpha-1,6-glucosidase , definition of oligo-alpha-1,6-glucosidase by Medical dictionary https://medical-dictionary. ... a href=https://medical-dictionary.thefreedictionary.com/oligo-alpha-1%2c6-glucosidase,oligo-alpha-1,6-glucosidase,/a,. * ...
How does an alpha glucosidase inhibitor control the blood-glucose level? What are its side effects? ... Alpha-glucosidase inhibitors mechanism of action. Alpha-Glucosidase inhibitors work by inhibiting alpha-glucosidase, an enzyme ... Side effects of alpha-glucosidase. Side effects of alpha-glucosidase inhibitors include flatulence (77%), diarrhea (33%), and ... What you can expect for Alpha-glucosidase inhibitors?. As a mono-therapy, alpha-glucosidase inhibitors can decrease your ...
  • Diabetes: Acarbose, an alpha-glucosidase inhibitor, competitively and reversibly inhibits alpha-glucosidase in the intestines. (wikipedia.org)
  • Luteolin has been found to be a strong inhibitor of alpha-glucosidase. (wikipedia.org)
  • Before you take an alpha-glucosidase inhibitor, tell all your doctors and your pharmacist about all the medicines you take. (howstuffworks.com)
  • If you have kidney impairment, you should not take an alpha-glucosidase inhibitor. (sharecare.com)
  • The first alpha-glucosidase inhibitor to become available in the United States was acarbose (Precose), which was approved by the U.S. Food and Drug Administration in 1995. (diabetesselfmanagement.com)
  • A second alpha-glucosidase inhibitor, miglitol (Glyset) became available in 1999. (diabetesselfmanagement.com)
  • The composition can include touchi extract and phaseolamin as the alpha-glucosidase and alpha-amylase inhibitor, respectively. (google.com)
  • More specifically, the invention relates to nutritional supplement compositions that include effective amounts of alpha-glucosidase and alpha-amylase inhibitors substantially in the absence of lipase inhibitor. (google.com)
  • The optimized anthocyanin-enriched extract was a stronger inhibitor of α-glucosidase than acarbose. (megazyme.com)
  • Effect of acarbose, an alpha-glucosidase inhibitor , on serum lipoprotein lipase mass levels and common carotid artery intima-media thickness in type 2 diabetes mellitus treated by sulfonylurea. (freethesaurus.com)
  • The alpha-glucosidase inhibitor l-blocks human immunodeficiency virus envelope glycopro-tein-mediated membrane fusion at the CXCR4 binding step. (freethesaurus.com)
  • The STOP-NIDDM Trial: an international study on the efficacy of an alpha-glucosidase inhibitor to prevent type 2 diabetes in a population with impaired glucose tolerance: rationale, design, and preliminary screening data. (freethesaurus.com)
  • The substrate analogue conduritol B epoxide (CBE) is demonstrated to be an active site-directed inhibitor of human lysosomal alpha-glucosidase. (antibody-antibodies.com)
  • However, ellagic acid, a potent alpha-glucosidase inhibitor possessing a planar structure, only slightly affected the activity of the blueberry wine samples, due to the lower concentration. (ac.rs)
  • Alpha glucosidase inhibitor voglibose can prevent pioglitazone-induced body weight gain in Type 2 diabetic patients. (ox.ac.uk)
  • Potent α-glucosidase inhibitor (IC 50 = 11 nM). (abcam.com)
  • Acarbose, an alpha-glucosidase inhibitor, improves insulin resistance in fructose-fed rats. (abcam.com)
  • Inhibition of disaccharide digestion in rat intestine by the alpha-glucosidase inhibitor acarbose (BAY g 5421). (abcam.com)
  • Hence, alpha-glucosidase inhibitors (like acarbose) are used as anti-diabetic drugs in combination with other anti-diabetic drugs. (wikipedia.org)
  • Alpha-glucosidase inhibitors (such as acarbose) decrease the absorption of carbohydrates from the digestive tract, thereby lowering the after-meal glucose levels. (medlineplus.gov)
  • Eight extracts inhibit both α-glucosidase and α-amylase better than acarbose as a positive control. (scialert.net)
  • The Role of Alpha-Glucosidase Inhibitors (Acarbose). (freethesaurus.com)
  • Alpha-glucosidase inhibitors (AGIs), such as acarbose (Precose) and miglitol (Glyset), block the absorption of ingested carbohydrates in the small intestine. (freethesaurus.com)
  • Results: Compared with acarbose used as a positive control (IC50 = 73.78 mu g/mL), all fruit wine samples exhibited higher alpha-glucosidase inhibitory activity. (ac.rs)
  • Acarbose was investigated in 30 trials, miglitol in seven, voglibose in one, and three trials compared different alpha-glucosidase inhibitors. (unboundmedicine.com)
  • Alpha-glucosidase inhibitors, such as acarbose (marketed as Precose or Glucobay) or miglitol (branded as Glyset) are drugs for type 2 diabetes which slow down the digestion of carbohydrates in the small intestine and therefore can help to reduce after meal blood sugar levels. (diabetes.co.uk)
  • Alpha-glucosidase inhibitors (acarbose [Precose], miglitol [Glyset]) do not enhance insulin secretion. (encyclopedia.com)
  • Acid alpha-glucosidase , also called α-1,4-glucosidase [5] and acid maltase , [6] is an enzyme ( EC 3.2.1.20 ) that helps to break down glycogen in the lysosome . (wikipedia.org)
  • An enzyme which removes the last 1,4-linked alpha-D-glucose residue from the nonreducing end of a long chain (or polymer) of such residues, making an alpha-D-glucose molecule out of it in the process. (everything2.com)
  • Other glucosidases include: Cellulase Beta-glucosidase Debranching enzyme Alpha-glucosidase hydrolyzes terminal non-reducing (1→4)-linked alpha-glucose residues to release a single alpha-glucose molecule. (wikipedia.org)
  • It was shown that for one of the trout liver alpha-glucosidases maximum activity of the enzyme was increased by 80% during exercise in comparison to a resting trout. (wikipedia.org)
  • Inhibitors of alpha-glucosidase show that the enzyme is involved in the pathway for N-glycans for viruses such as HIV and human hepatitis B virus (HBV). (wikipedia.org)
  • The enzyme catalyses the following chemical reaction Endohydrolysis of (1->3)-alpha-D-glucosidic linkages in isolichenan, pseudonigeran and nigeran Products from pseudonigeran (1,3-alpha-D-glucan) are nigerose and alpha-D-glucose. (wikipedia.org)
  • Lysosomal alpha-glucosidase may refer to: Glucan 1,4-a-glucosidase, an enzyme Acid alpha-glucosidase, an enzyme This set index page lists enzyme articles associated with the same name. (wikipedia.org)
  • Alpha-glucosidase is an intestinal enzyme that breaks down a variety of carbohydrates, but two of the more important ones are sucrose (table sugar), which is broken down into glucose and fructose, and lactose (milk sugar), which is broken down into glucose and galactose. (diabetesselfmanagement.com)
  • Recently, enzyme enhancement therapy (EET) for Pompe disease involving imino sugars, which act as potential inhibitors of acid alpha-glucosidases in vitro, to improve the stability and/or transportation of mutant acid alpha-glucosidases in cells was studied and attracted interest. (nih.gov)
  • However, the purified enzyme did not show alpha-xylosidase activity. (mdpi.com)
  • Alpha-Glucosidase inhibitors work by inhibiting alpha-glucosidase, an enzyme located in the proximal gut (small-intestinal) epithelium that breaks down disaccharides and carbohydrates that are more complex. (healthy-ojas.com)
  • One Unit of α-glucosidase activity is defined as the amount of enzyme required to produce one µmole of p -nitrophenol from p NP-α-Glucosidase (10 mM) in sodium phosphate buffer (100 mM), pH 6.8 at 40 o C. (megazyme.com)
  • High purity α-Glucosidase (yeast maltase) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. (megazyme.com)
  • One Unit of α-Glucosidase activity is the amount of enzyme required to release one µmole of p -nitrophenol per minute from the appropriate substrate at pH 6.5 at 40 o C. (megazyme.com)
  • High purity α-Glucosidase ( Bacillus stearothermophilus ) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. (megazyme.com)
  • The development and recent approval of recombinant acid alpha-glucosidase for enzyme replacement therapy have been major milestones in Pompe disease research. (elsevier.com)
  • Acid alpha-glucosidase is the enzyme responsible for degradation of glycogen polymers to glucose in the acidic milieu of the lysosomes. (elsevier.com)
  • Measurement of enzyme activities also showed that alpha-glucosidase, unlike other lysosomal enzymes, is mainly secreted via the apical pathway. (uu.nl)
  • METHODS: The evaluation of enzyme inhibitory activity of seven Australian aboriginal medicinal plants and five Indian Ayurvedic plants was carried out against α-amylase and α-glucosidase. (isharonline.org)
  • The heterodimeric enzyme glucosidase II plays a role in protein folding and quality control by cleaving glucose residues from immature glycoproteins in the endoplasmic reticulum. (nih.gov)
  • Alpha-glucosidase is an enzyme that is produced in the epididymis. (ivfriga.eu)
  • Water solubility of 6-O-(alpha-D: -glucopyranosyl)alpha-tocopherol was 26 g/l at 25 degrees C. alpha-Tocopheryl glycosides showed antioxidant activities with IC(50) values from 0.5 to 1 mM and angiotensin-converting enzyme (ACE) inhibitory activity with IC(50) values from 1.3 to 2.6 mM. (cftri.com)
  • Their binding to the mammalian alpha-glucosidase and their effect on enzyme activity were evaluated. (uni-lj.si)
  • Glucosidase is an enzyme in your body that helps convert complex carbohydrates to simple sugars. (healthline.com)
  • Alpha-glucosidase is an enzyme that is capable of breaking down carbohydrates into shorter sugar particles like glucose, in sequence for the carbohydrates to be absorbed.Alpha-glucosidase inhibitors (AGIs), sometimes pointed to as starch blockers are anti-diabetic drugs that further to diminish post-meal blood glucose levels. (howtorelief.com)
  • The GAA gene provides instructions for producing an enzyme called acid alpha-glucosidase (also known as acid maltase). (nih.gov)
  • Mutations in this gene significantly reduce the activity of acid alpha-glucosidase, preventing the enzyme from breaking down glycogen effectively. (nih.gov)
  • This study focused on the synthesis and characterization of hydrazide ligands and their respective Pd(II) complexes and used high throughput screening to determine their α-glucosidase and carbonic anhydrase II enzyme inhibition activities. (edu.qa)
  • Neutral alpha-glucosidase C is an enzyme that in humans is encoded by the GANC gene. (wikipedia.org)
  • This enzyme hydrolyses terminal, non-reducing 1,4-linked alpha-D-glucose residues and releases alpha-D-glucose. (wikipedia.org)
  • F. Ye, Z. Shen, and M. Xie, "Alpha-glucosidase inhibition from a Chinese medical herb ( Ramulus mori ) in normal and diabetic rats and mice," Phytomedicine , vol. 9, no. 2, pp. 161-166, 2002. (hindawi.com)
  • V. Ghadyale, S. Takalikar, V. Haldavnekar, and A. Arvindekar, "Effective control of postprandial glucose level through inhibition of intestinal alpha glucosidase by Cymbopogon martinii (Roxb. (hindawi.com)
  • The 75% ethanol extracts of propolis (75% EEP) showed the highest inhibitory effect on α -glucosidase and sucrase and were a noncompetitive inhibition mode. (hindawi.com)
  • We examined the inhibitory and binding effects of four imino sugars on a recombinant human acid alpha-glucosidase, alglucosidase alfa, by means of inhibition assaying and isothermal titration calorimetry (ITC). (nih.gov)
  • One of the strategies is maintaining postprandial glucose level through inhibition of α-amylase and α-glucosidase and preventing the hydrolysis of Glucagon Like Peptide-1 (GLP-1) through inhibition of Dipeptidyl peptidase IV. (scialert.net)
  • This study showed us that some extracts have inhibition activity of α-glucosidase, α-amylase and DPP-IV. (scialert.net)
  • One therapeutic approach for treating diabetes is decreasing post-prandial hyperglycemia by delaying glucose absorption through carbohydrate-hydrolyzing enzymes inhibition, α-glucosidase and α-amylase in the digestive tract. (scialert.net)
  • The GPL water-soluble extract possesses the potential effect of inhibition on the alpha-glucosidase activity from the small intestinal mucosa of diabetic mouse. (mysciencework.com)
  • A competitive mode of inhibition is obtained with glycogen as natural and 4-methylumbelliferyl-alpha-D-glucopyranoside as artificial substrate. (antibody-antibodies.com)
  • Alpha-glucosidase inhibitors work by ambitious and reversible inhibition of those intestinal enzymes. (howtorelief.com)
  • Evaluation of alpha-glucosidase inhibition by using an immobilized assay system. (abcam.com)
  • A new series of 2-substituted quinazolin-4(3H)-one derivatives including coumarin nucleus has been synthesized and screened for their lipase and a-glucosidase inhibition properties. (erdogan.edu.tr)
  • Alpha-glucosidase inhibitors work by stopping the enzymes that digest the starches that you eat. (howstuffworks.com)
  • Enzymes that catalyze the exohydrolysis of 1,4-alpha-glucosidic linkages with release of alpha- Glucose . (online-medical-dictionary.org)
  • Alpha-glucosidases are a series of enzymes, including sucrase and maltase, located in the brush-border surface of intestinal cell, which catalyze the final step in the digestive process of carbohydrates to release absorbable monosaccharides resulting in increased blood glucose levels [ 4 ]. (hindawi.com)
  • Scientists have found compounds that inhibit enzymes responsible for carbohydrate absorption and assimilation similar to the alpha-glucosidase inhibitors. (diabetesincontrol.com)
  • The neopullulanase and alpha-glucosidase appeared to be the main enzymes involved in the breakdown of starch, because they were responsible for most of the starch-degrading activity detected in B. thetaiotaomicron cell extracts. (asm.org)
  • To determine the importance of these enzymes in the starch utilization pathway, we cloned the genes encoding the neopullulanase and alpha-glucosidase. (asm.org)
  • Glucosidases are cellular enzymes, able to split polysaccharides into glucose. (eurekamag.com)
  • Taste cell-expressed a-glucosidase enzymes contribute to gustatory responses to disaccharides. (viictr.org)
  • By blocking these enzymes, by alpha-glucosidase inhibitors can slow down the digestion of carbohydrates, so that glucose from food enters the bloodstream more slowly, thus decreasing the rise in blood glucose levels following eating. (howtorelief.com)
  • Glycosylases are a group of enzymes that includes glucosidases, mannosidases and heparanases. (genecards.org)
  • In humans, two types of digestive enzymes, α-amylase and mucosal α-glucosidase, are involved in breaking down starch molecules to absorbable glucose. (uidaho.edu)
  • The enzymes designated alpha-glucosidases A1 and A2 had native molecular weights of approximately 191.23± 0.7 and 140.39± 1.2 kDa, respectively, and there functioned as monomeric structures. (advancejournals.org)
  • The enzymes readily hydrolyzed p-nitrophenyl-α-D-glucoside, maltose, maltodextrins and required strictly alpha-gluco configuration for activity. (advancejournals.org)
  • EC 3.2.1.20) are a widespread group of enzymes that catalyze the hydrolysis of the alpha-glucosidic bond from the non-reducing end of a chain as well as the alpha -glucosidic bond of free disaccharides [1;2]. (advancejournals.org)
  • Glucosidase enzymes are involved in several biological processes such as the intestinal digestion, the biosynthesis of glycoproteins and the lysosomal catabolism of the glycoconjugates [5]. (advancejournals.org)
  • Pompe disease is a progressive metabolic neuromuscular disorder resulting from deficiency of lysosomal acid alpha-glucosidase (GAA). (nih.gov)
  • The human adenocarcinoma cell line Caco-2 was used for studies on the biosynthesis and transport of lysosomal acid alpha-glucosidase in polarized epithelial cells. (uu.nl)
  • Lysosomal acid alpha-glucosidase consists of four different peptides processed from a single chain precursor. (nih.gov)
  • Midgut alpha-glucosidase (EC 3.2.1.20) activities were measured after ingestion of blood and sugar meals by the phlebotomine sandfly Phlebotomus langeroni. (lancs.ac.uk)
  • Mutations in GANAB, Encoding the Glucosidase II? (nih.gov)
  • GANAB (Glucosidase II Alpha Subunit) is a Protein Coding gene. (genecards.org)
  • Glycogen storage disease type II, also called Pompe disease: a disorder in which alpha-glucosidase is deficient. (wikipedia.org)
  • In 2006, the drug alglucosidase alfa became the first released treatment for Pompe disease and acts as an analog to alpha-glucosidase. (wikipedia.org)
  • Recombinant human acid alpha-glucosidase is safe and effective for treatment of infantile-onset Pompe disease. (nih.gov)
  • Pompe disease is an inherited condition of acid alpha-glucosidase (GAA) deficiency resulting in lysosomal accumulation of glycogen in all tissues. (clinicaltrials.gov)
  • Pompe disease, a rare lysosomal storage disease caused by deficiency of the lysosomal acid α-glucosidase (GAA), is characterized by glycogen accumulation, triggering severe secondary cellular damage and resulting in progressive motor handicap and premature death. (nih.gov)
  • Yeast and rat small intestinal alpha-glucosidases have been shown to be inhibited by several groups of flavonoids. (wikipedia.org)
  • Dietary starch breakdown product sensing mobilizes and apically activates a-glucosidases in small intestinal enterocytes. (viictr.org)
  • Contribution of the Individual Small Intestinal a-Glucosidases to Digestion of Unusual a-Linked Glycemic Disaccharides. (viictr.org)
  • Although its functions look similar to human small intestinal α-glucosidase, its hydrolytic mechanism and influence in physiologic responses are quite different. (uidaho.edu)
  • Recombinant protein encompassing a sequence within the N-terminus region of human alpha Glucosidase II. (genetex.com)
  • alpha Glucosidase II antibody [N1N2], N-term detects alpha Glucosidase II protein at cytoplasm in human breast carcinoma by immunohistochemical analysis. (genetex.com)
  • The peptides appeared to originate from a protein domain which is highly conserved among alpha-amylases, maltase, glucoamylases, and transglucanosylases. (antibody-antibodies.com)
  • GAA (Glucosidase Alpha, Acid) is a Protein Coding gene. (genecards.org)
  • Many of these mutations change one of the protein building blocks (amino acids) used to make acid alpha-glucosidase. (nih.gov)
  • In this article, we review the structure and properties of the small intestine α-glucosidases, including their protein structure, hydrolytic activity, digestion capability, distribution in the small intestine, and the dietary influence in their activity and synthesis. (uidaho.edu)
  • Alpha-glucosidase inhibitors (AGIs) are a class of drugs used to treat type 2 diabetes . (diabetesdaily.com)
  • Alpha-glucosidase inhibitors (AGIs), sometimes referred to as starch blockers, are anti-diabetic medicines that help to reduce post meal blood glucose levels . (diabetes.co.uk)
  • OBJECTIVE -To review the effects of monotherapy with α-glucosidase inhibitors (AGIs) for patients with type 2 diabetes, with respect to mortality, morbidity, glycemic control, insulin levels, plasma lipids, body weight, and side effects. (diabetesjournals.org)
  • The use of alpha-glucosidase inhibitors results in a lower, delayed rise in blood glucose after a meal. (diabetesselfmanagement.com)
  • Alpha-glucosidase inhibitors are particularly useful for managing mild hyperglycemia (high blood glucose) in newly diagnosed people with Type 2 diabetes , as well as for managing after-meal hyperglycemia. (diabetesselfmanagement.com)
  • Alpha-glucosidase inhibitors are suitable for people with type 2 diabetes with difficulty controlling blood glucose levels following meals. (diabetes.co.uk)
  • However, while alpha-glucosidase inhibitors themselves do not cause low blood glucose, when they are used in combination with another diabetes medicines, hypoglycemia can occur. (diabetesselfmanagement.com)
  • Alpha-glucosidase inhibitors are diabetes medication, which slows down carbohydrate digestion and limits blood-glucose rise after food, a common problem in diabetics. (healthy-ojas.com)
  • Alpha-glucosidase breaks down starch and disaccharides to glucose. (wikipedia.org)
  • Alpha-glucosidase inhibitors , a class of drugs also known as "starch blockers," function by slowing the absorption of certain carbohydrates in the gastrointestinal tract. (diabetesselfmanagement.com)
  • Contribution of a neopullulanase, a pullulanase, and an alpha-glucosidase to growth of Bacteroides thetaiotaomicron on starch. (asm.org)
  • Disruption of the alpha-glucosidase gene, susB, reduced the rate of growth on starch only slightly. (asm.org)
  • The methanol and aqueous extracts were evaluated for their in vitro alpha-glucosidase inhibitory activity and an oral starch tolerance test. (bvsalud.org)
  • The physiological role of the two alpha-glucosidases in the digestive tract of the termite could be the digestion of di-and oligosaccharides derived from starch. (advancejournals.org)
  • Deficiency of alpha-1,4- Glucosidase may cause Glycogen Storage Disease Type II . (online-medical-dictionary.org)
  • Loss of function mutations lead to acid alpha-glucosidase deficiency thus glycogen accumulates in lysosomes and cytoplasm. (cags.org.ae)
  • Seasonal changes of neutral alpha-glucosidase activity in huma. (mysciencework.com)
  • In males being treated for infertility, neutral alpha-glucosidase activity correlated with the percentage of sperm DNA fragmentation. (nih.gov)
  • What should I know before taking alpha-glucosidase inhibitors? (sharecare.com)
  • This gene encodes lysosomal alpha-glucosidase , which is essential for the degradation of glycogen to glucose in lysosomes . (wikipedia.org)
  • This gene encodes the alpha subunit of glucosidase II and a member of the glycosyl hydrolase 31 family of proteins. (nih.gov)
  • Taken with the first bite of a meal, alpha-glucosidase inhibitors are especially well-suited to treat postprandial hyperglycemia (a sharp rise in blood sugar after meals), a common and serious problem faced by many people with Type 2 diabetes. (diabetesselfmanagement.com)
  • Alpha-glucosidase inhibitors are suitable as mono-therapy or in combination with sulfonylureas for management of type 2 diabetes. (healthy-ojas.com)
  • In patients with type 1 diabetes, insulin is used, whereas in patients with type 2 diabetes, sulfonylurea, biguanides, meglitinides, dipeptidyl-peptidase-4 (DPP-4) inhibitors, alpha-glucosidase inhibitors , sodium glucose cotransporter 2 inhibitors (SGLT2), and glucagon-like peptide-1 (GLP-1) agonist are used. (freethesaurus.com)
  • The traditional use of E. dioeca to control type 2 diabetes was confirmed, being an important source of alfa- glucosidase inhibitors. (bvsalud.org)
  • It is unclear whether alpha-glucosidase inhibitors influence mortality or morbidity in patients with type 2 diabetes. (unboundmedicine.com)
  • 1 . Van de Laar FA, Lucassen PL, Akkermans RP, Van de Lisdonk EH, Rutten GE, Van Weel C. Alpha-glucosidase inhibitors for type 2 diabetes mellitus. (unboundmedicine.com)
  • Alpha-glucosidase inhibitors for type 2 diabetes mellitus is a sample topic from the Evidence-Based Medicine Guidelines . (unboundmedicine.com)
  • Evidence Central , evidence.unboundmedicine.com/evidence/view/EBMG/453532/all/Alpha_glucosidase_inhibitors_for_type_2_diabetes_mellitus. (unboundmedicine.com)
  • Alpha-glucosidase inhibitors for type 2 diabetes mellitus: Cochrane systematic review is a topic covered in the Cochrane Abstracts . (unboundmedicine.com)
  • Cochrane Abstracts , Evidence Central , evidence.unboundmedicine.com/evidence/view/Cochrane/432480/all/Alpha_glucosidase_inhibitors_for_type_2_diabetes_mellitus:_Cochrane_systematic_review. (unboundmedicine.com)
  • Immunohistochemical analysis of alpha Glucosidase II in paraffin-embedded SW480 xenograft, using ab96757 at 1/500 dilution. (abcam.com)
  • Immunohistochemical analysis of paraffin-embedded SW480 xenograft , using alpha Glucosidase II(GTX102237) antibody at 1:500 dilution. (genetex.com)
  • The objective of the present study was to evaluate the inhibitory properties of various extracts of propolis on alpha-glucosidase from baker's yeast and mammalian intestine. (hindawi.com)
  • No residual alpha-glucosidase activity was detectable in extracts from this strain. (asm.org)
  • Azoospermia: Diagnosis of azoospermia has potential to be aided by measurement of alpha-glucosidase activity in seminal plasma. (wikipedia.org)
  • α -Glucosidase inhibitory activity of Syzygium cumini (Linn. (hindawi.com)
  • Glycogen storage disease type II (GSD-II) is a lethal, autosomal recessive metabolic myopathy caused by a lack of acid-alpha-glucosidase (GAA) activity in the cardiac and skeletal muscles. (unboundmedicine.com)
  • alpha-Glucosidase activity increased significantly within 1 hr after a blood meal and was maintained at significantly high activities until 48 hr postfeeding, when it fell to basal activity levels. (lancs.ac.uk)
  • Midgut alpha-glucosidase activity also increased within 1 hr of feeding on a sucrose meal, but there was no discernable peak in activity during the days postingestion. (lancs.ac.uk)
  • Nearly 90% of the alpha-glucosidase activity was associated with midgut cells of sugar fed sandflies compared to only 46% in blood fed insects. (lancs.ac.uk)
  • No alpha-glucosidase activity was detected in the crop, indicating chat glycosidases originate from the midgut epithelium rather than the salivary glands. (lancs.ac.uk)
  • The aim of this study was to determine in vitro inhibitory activity of α-glucosidase, α-amylase and Dipeptidyl peptidase IV from 42 ethanolic extract s of selected Indonesian plants. (scialert.net)
  • α-amylase activity was measured at λ = 540 nm, α-glucosidase activity and the DPP-IV activity were measured at λ = 405 nm. (scialert.net)
  • This study was conducted to find inhibitory activity of α-amylase, α-glucosidase and DPP-IV from Indonesian plants. (scialert.net)
  • Although there are contradictory reports, the biochemical evaluation of the seminal activity of neutral alpha-glucosidase (NAG) has repeatedly been described as an important parameter to test epididymal patency and is recommended by the WHO. (mysciencework.com)
  • Thus, our data clearly demonstrate circannual changes of the seminal activity of neutral alpha-glucosidase. (mysciencework.com)
  • Effect of Psidium guajava leaf extract on alpha-glucosidase activity in small intestine of diabetic mouse]. (mysciencework.com)
  • glucosidase inhibitory activity by different ethanol-aqueous extractions from the dried fruits of Terminalia chebula Retz. (isharonline.org)
  • The level of activity of alpha-glucosidase depends on the secretory function of the epididymis. (ivfriga.eu)
  • Reduced activity of alpha-glucosidase may be a sign of a complete or partial obstruction of the epididymis and ejaculatory ducts caused by inflammation or infection. (ivfriga.eu)
  • Objectives: Potential alpha-glucosidase inhibitory activity of fruit wines made from blueberry, black chokeberry, blackberry, raspberry and sour cherry was the subject of this study. (ac.rs)
  • tly contributed to alpha-glucosidase inhibitory activity of the blueberry, black chokeberry and sour cherry wine samples. (ac.rs)
  • Both forms of neutral alpha-glucosidase I and 2 from human urine exhibited the maximal activity at pH 5.75 = 6.5, had a similar Km value (0.73 mM) with maltose as a substrate and did not differ in their properties from the corresponding forms of neutral alpha-glucosidase in a soluble fraction from human kidney. (msk.ru)
  • GO annotations related to this gene include carbohydrate binding and alpha-1,4-glucosidase activity . (genecards.org)
  • The alpha-glucosidase inhibitory activity is related with the presence of glycosides , phenolic compounds and flavonoids . (bvsalud.org)
  • The two isoforms isolated exhibited maximal alpha-glucosidase activity pH at 5.6. (advancejournals.org)
  • Type I alpha-glucosidase hydrolyzes heterogeneous substrates (e.g. synthetic alpha-glucosides and sucrose) more rapidly than homogeneous substrates (e.g. maltooligosaccharides), whereas types II and III display higher activity toward homogeneous than toward heterogeneous substrates. (advancejournals.org)
  • eremostachys laevigata bunge, silene aucheriana boiss exhibited more than 60% alpha-glucosidase inhibitory activity. (gov.ir)
  • results: among them, descurania sophia, fumaria vailantii and ferula haussknechti exhibited significant alpha glucosidase inhibitory activity with ic50 values of 0.9 µg/ml, 24.55 µg/ml and 2.71 µg/ml, respectively. (gov.ir)
  • The ethanolic extract was first separated into petroleum ether, ethyl acetate, n-butanol and water soluble fractions and screened for inhibitory activity against α-glucosidase. (semanticscholar.org)
  • Alpha-glucosidase inhibitors are particularly useful in postprandial hyperglycemia. (healthy-ojas.com)
  • The functional importance of Asp-518 and other residues around the catalytic site was studied by expression of in vitro mutagenized alpha-glucosidase cDNA in transiently transfected COS cells. (antibody-antibodies.com)
  • En dicho marco, se evaluó tanto la actividad inhibitoria de alfa-glucosidasa in vitro , como su desempeño en una prueba de tolerancia a una carga de almidón postprandial. (bvsalud.org)
  • Moreover, computational analyses for the detection of functional domains, active and catalytic sites, as well as 3D structural predictions revealed a close relationship with an E. coli YicI-like alpha-xylosidase of the GH31 family. (mdpi.com)
  • CBE-labeled peptides containing the catalytic residue of lysosomal alpha-glucosidase were isolated and identified by microsequencing and amino acid analysis. (antibody-antibodies.com)
  • The catalytic efficiency (Vmax/ KM) values for p-nitrophenyl-α-D-glucoside, were respectively 277.75±9.2 and 59.51±4, for alpha-glucosidases A1 and A2. (advancejournals.org)
  • Trout liver alpha-glucosidases were extracted and characterized. (wikipedia.org)
  • In the present study, screening of peptide libraries was used to select peptides with an affinity towards mammalian intestinal alpha-glucosidase as potential leads in antidiabetic agent development. (uni-lj.si)
  • Two linear and two cyclic heptapeptides with high affinity towards intestinal alpha-glucosidase were selected. (uni-lj.si)
  • To our knowledge, this is the first report on the characterization of an alpha-glucosidase from the halophilic Archaea, which could serve as a new model to gain insights into carbon metabolism in this understudied microbial group. (mdpi.com)
  • Characterization of the human lysosomal alpha-glucosidase gene. (nih.gov)
  • In present study, purification and characterization of two alpha-glucosidases from termite workers Macrotermes bellicosus (Termitidae: Macrotermitinae) were conducted. (advancejournals.org)
  • Alpha-glucosidase is a carbohydrate-hydrolase that releases alpha-glucose as opposed to beta-glucose. (wikipedia.org)
  • By blocking the absorption of carbohydrate into the bloodstream, alpha-glucosidase inhibitors leave more carbohydrate available to the gastrointestinal flora (bacteria that live in the intestines). (diabetesselfmanagement.com)
  • glucosidase may function in cell physiology and carbohydrate metabolism. (eurekamag.com)
  • Other drugs that are not included in the algorithm but may benefit certain individuals include DPP-4 inhibitors, meglitinides, alpha-glucosidase inhibitors, colesevelam, and bromocriptine. (diabetesselfmanagement.com)
  • The studies enhanced knowledge of the mechanism by which alpha-glucosidase binds to imino sugars. (wikipedia.org)
  • Binding parameters and thermodynamics of the interaction of imino sugars with a recombinant human acid alpha-glucosidase (alglucosidase alfa): insi. (nih.gov)
  • Binding parameters and thermodynamics of the interaction of imino sugars with a recombinant human acid alpha-glucosidase (alglucosidase alfa): insight into the complex formation mechanism. (nih.gov)
  • These biochemical and structural analyses increased our understanding of the molecular interaction between a human acid alpha-glucosidase and imino sugars. (nih.gov)
  • However, alpha-glucosidase inhibitors don't stop simple sugars (found in foods such as fruit, desserts, candy, and honey) from passing into your blood. (healthline.com)
  • It was found that substituting Asp-513 with Glu-513 interferes with posttranslational modification and intracellular transport of alpha-glucosidase's precursor. (wikipedia.org)
  • Cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins. (abcam.com)
  • Via an intermediate form of 95 x 10(3) Mr, this precursor is processed into a mature form of 76 x 10(3) Mr. Combination of metabolic labelling with subcellular fractionation showed that the 112 x 10(3) Mr precursor of alpha-glucosidase is transported to the lysosomes. (uu.nl)
  • Immunoprecipitation of alpha-glucosidase from culture medium derived from either the apical or basolateral site of radiolabelled Caco-2 cells, showed that 70-80% of the total amount of precursor form present in the medium is secreted from the apical membrane. (uu.nl)
  • Furthermore, immunocytochemistry showed the presence of a precursor form of alpha-glucosidase on the apical, but not the basolateral, membrane of the Caco-2 cells. (uu.nl)
  • Substitution of Asp-513 by Glu-513 is shown to interfere with the posttranslational modification and the intracellular transport of the alpha-glucosidase precursor. (antibody-antibodies.com)
  • This precursor is then transferred en bloc on neo-synthesized proteins through the action of the oligosaccharyltransferase giving birth to glycoproteins. (springer.com)
  • Alpha-glucosidase inhibitors as one of therapeutic approaches for diabetes mellitus have been known since the early 1990s [ 5 ]. (hindawi.com)
  • This is in contrast to beta-glucosidase. (wikipedia.org)
  • Enzymatic syntheses of water-soluble alpha-tocopheryl glycosides were carried out in di-isopropyl ether using amyloglucosidase from Rhizopus mold or beta-glucosidase isolated from sweet almond. (cftri.com)
  • and for the beta-glucosidase: alpha-tocopherol 0.5 mmol, D: -glucose 0.5 mmol, 110 AU beta-glucosidase, 0.1 mM pH 6 phosphate buffer and 72 h. (cftri.com)
  • Human lysosomal alpha-glucosidase. (wikipedia.org)
  • The gene coding for human lysosomal alpha-glucosidase is about 20 kb long and its structure has been cloned and confirmed. (wikipedia.org)
  • Human lysosomal alpha-glucosidase has been studied for the significance of the Asp-518 and other residues in proximity of the enzyme's active site. (wikipedia.org)
  • Recombinant fragment containing a sequence corresponding to a region within amino acids 97-261 of Human alpha Glucosidase II (NP_938149). (abcam.com)
  • The young age at which these patients initiated therapy may have contributed to their improved response compared to previous trials with recombinant human acid alpha-glucosidase in which patients were older. (nih.gov)
  • Our alpha Glucosidase 2 Lysates can be used in a variety of model species: Human. (novusbio.com)
  • The influence of inflammation of the human male genital tract on secretion of the seminal markers alpha-glucosidase, glycerophosphocholine, carnitine, fructose and citric acid. (biomedsearch.com)
  • GENTAUR antibody-antibodies.com The Marketplace for Antibodies : Human lysosomal alpha-glucosidase. (antibody-antibodies.com)
  • Multiple forms of neutral alpha-glucosidase were found in human urine. (msk.ru)
  • Yan B, Raben N, Plotz P. The human acid alpha-glucosidase gene is a novel target of the Notch-1/Hes-1 signaling pathway. (nih.gov)
  • A nutritional supplement composition contains inhibitors of alpha-glucosidase and alpha-amylase substantially in the absence of lipase inhibitors. (google.com)
  • In their study, which was published in the Journal of Dietary Supplements , they examined the effect of spirulina extract on lipase and alpha-glucosidase activities. (naturalnews.com)
  • objective: alpha-glucosidase, as a carbohydratase catalyses the liberation of alpha-glucose from the nonreducing end of some carbohydrates from foods in digestive tract. (gov.ir)
  • La actividad inhibitoria de alfa-glucosidasa, reflejada en ambas pruebas, está relacionada con la presencia de glicósidos, compuestos fenólicos y flavonoides . (bvsalud.org)
  • Alpha-glucosidase inhibitors have a significant effect on glycemic control and insulin levels, but no statistically significant effect on lipids and body weight. (unboundmedicine.com)
  • however, the respective Pd(II)-hydrazide complexes were approximately 300 times more potent α-glucosidase inhibitors than the standard compound, 1-deoxynojirimycin (DNJ). (edu.qa)
  • glucosidase and Glucose-6-phosphatase appear to be very similar, i.e., closely linked to the endoplasmic reticulum, and are some what related to the particular glycogen. (eurekamag.com)
  • Among the synthesized compounds, N'-{2-[2-(3,4-dichlorobenzyl)-4-oxoquinazolin-3(4H)-yl]acetyl}-2-oxo-2H-chromene-3-carbohydrazide and N'-{2-[2-(4-bromobenzyl)-4-oxoquinazolin-3(4H)-yl] acetyl}-2-oxo-2H-chromene-3-carbohydrazide showed the best inhibitory effect against a-glucosidase with IC50 values of 6.11 +/- 0.40 and 7.34 +/- 0.37 mu M, respectively. (erdogan.edu.tr)