A heterogeneous family of water-soluble structural proteins found in cells of the vertebrate lens. The presence of these proteins accounts for the transparency of the lens. The family is composed of four major groups, alpha, beta, gamma, and delta, and several minor groups, which are classed on the basis of size, charge, immunological properties, and vertebrate source. Alpha, beta, and delta crystallins occur in avian and reptilian lenses, while alpha, beta, and gamma crystallins occur in all other lenses.
A transparent, biconvex structure of the EYE, enclosed in a capsule and situated behind the IRIS and in front of the vitreous humor (VITREOUS BODY). It is slightly overlapped at its margin by the ciliary processes. Adaptation by the CILIARY BODY is crucial for OCULAR ACCOMMODATION.
A class of crystallins that provides refractive power and translucency to the lens (LENS, CRYSTALLINE) in VERTEBRATES. Beta-crystallins are similar in structure to GAMMA-CRYSTALLINS in that they both contain Greek key motifs. Beta-crystallins exist as oligomers formed from acidic (BETA-CRYSTALLIN A CHAIN) and basic (BETA-CRYSTALLIN B CHAIN) subunits.
The basic subunit of beta-crystallins.
A subclass of crystallins that found in the lens (LENS, CRYSTALLINE) of VERTEBRATES. Gamma-crystallins are similar in structure to BETA-CRYSTALLINS in that they both form into a Greek key-like structure. They are composed of monomeric subunits.
The acidic subunit of beta-crystallins.
A subclass of crystallins that provides the majority of refractive power and translucency to the lens (LENS, CRYSTALLINE) in VERTEBRATES. Alpha-crystallins also act as molecular chaperones that bind to denatured proteins, keep them in solution and thereby maintain the translucency of the lens. The proteins exist as large oligomers that are formed from ALPHA-CRYSTALLIN A CHAIN and ALPHA-CRYSTALLIN B CHAIN subunits.
Partial or complete opacity on or in the lens or capsule of one or both eyes, impairing vision or causing blindness. The many kinds of cataract are classified by their morphology (size, shape, location) or etiology (cause and time of occurrence). (Dorland, 27th ed)
One of the subunits of alpha-crystallins. Unlike ALPHA-CRYSTALLIN B CHAIN the expression of ALPHA-CRYSTALLIN A CHAIN is limited primarily to the lens (LENS, CRYSTALLINE).
One of the alpha crystallin subunits. In addition to being expressed in the lens (LENS, CRYSTALLINE), alpha-crystallin B chain has been found in a variety of tissues such as HEART; BRAIN; MUSCLE; and KIDNEY. Accumulation of the protein in the brain is associated with NEURODEGENERATIVE DISEASES such as CREUTZFELDT-JAKOB SYNDROME and ALEXANDER DISEASE.
The core of the crystalline lens, surrounded by the cortex.
A superorder in the class CEPHALOPODA, consisting of the orders Octopoda (octopus) with over 200 species and Vampyromorpha with a single species. The latter is a phylogenetic relic but holds the key to the origins of Octopoda.
Plasma glycoprotein member of the serpin superfamily which inhibits TRYPSIN; NEUTROPHIL ELASTASE; and other PROTEOLYTIC ENZYMES.
Electrophoresis in which a second perpendicular electrophoretic transport is performed on the separate components resulting from the first electrophoresis. This technique is usually performed on polyacrylamide gels.
One of the two major pharmacological subdivisions of adrenergic receptors that were originally defined by the relative potencies of various adrenergic compounds. The alpha receptors were initially described as excitatory receptors that post-junctionally stimulate SMOOTH MUSCLE contraction. However, further analysis has revealed a more complex picture involving several alpha receptor subtypes and their involvement in feedback regulation.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
The family of true toads belonging to the order Anura. The genera include Bufo, Ansonia, Nectophrynoides, and Atelopus.
Hypoxia-inducible factor 1, alpha subunit is a basic helix-loop-helix transcription factor that is regulated by OXYGEN availability and is targeted for degradation by VHL TUMOR SUPPRESSOR PROTEIN.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The gradual irreversible changes in structure and function of an organism that occur as a result of the passage of time.
The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
The determination of the concentration of a given component in solution (the analyte) by addition of a liquid reagent of known strength (the titrant) until an equivalence point is reached (when the reactants are present in stoichiometric proportions). Often an indicator is added to make the equivalence point visible (e.g., a change in color).
The removal of an amino group (NH2) from a chemical compound.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.
A family of cellular proteins that mediate the correct assembly or disassembly of polypeptides and their associated ligands. Although they take part in the assembly process, molecular chaperones are not components of the final structures.
A superorder of CEPHALOPODS comprised of squid, cuttlefish, and their relatives. Their distinguishing feature is the modification of their fourth pair of arms into tentacles, resulting in 10 limbs.
Animals that have no spinal column.
Maf proto-oncogene protein is the major cellular homolog of the V-MAF ONCOGENE PROTEIN. It was the first of the mammalian MAF TRANSCRIPTION FACTORS identified, and it is induced in activated T-LYMPHOCYTES and regulates GENETIC TRANSCRIPTION of INTERLEUKIN-4. c-maf is frequently translocated to an immunoglobulin locus in MULTIPLE MYELOMA.
A member of the NICOTINIC ACETYLCHOLINE RECEPTOR subfamily of the LIGAND-GATED ION CHANNEL family. It consists entirely of pentameric a7 subunits expressed in the CNS, autonomic nervous system, vascular system, lymphocytes and spleen.
A genus of livebearing cyprinodont fish comprising the guppy and molly. Some species are virtually all female and depend on sperm from other species to stimulate egg development. Poecilia is used in carcinogenicity studies as well as neurologic and physiologic research.
One of a group of nonenzymatic reactions in which aldehydes, ketones, or reducing sugars react with amino acids, peptides, or proteins. Food browning reactions, such as those that occur with cooking of meats, and also food deterioration reactions, resulting in decreased nutritional value and color changes, are attributed to this reaction type. The Maillard reaction is studied by scientists in the agriculture, food, nutrition, and carbohydrate chemistry fields.
Measurement of the index of refraction (the ratio of the velocity of light or other radiation in the first of two media to its velocity in the second as it passes from one into the other).
Disruption of the non-covalent bonds and/or disulfide bonds responsible for maintaining the three-dimensional shape and activity of the native protein.
The diversion of RADIATION (thermal, electromagnetic, or nuclear) from its original path as a result of interactions or collisions with atoms, molecules, or larger particles in the atmosphere or other media. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
A mass spectrometric technique that is used for the analysis of large biomolecules. Analyte molecules are embedded in an excess matrix of small organic molecules that show a high resonant absorption at the laser wavelength used. The matrix absorbs the laser energy, thus inducing a soft disintegration of the sample-matrix mixture into free (gas phase) matrix and analyte molecules and molecular ions. In general, only molecular ions of the analyte molecules are produced, and almost no fragmentation occurs. This makes the method well suited for molecular weight determinations and mixture analysis.
Cell surface receptor for LAMININ, epiligrin, FIBRONECTINS, entactin, and COLLAGEN. Integrin alpha3beta1 is the major integrin present in EPITHELIAL CELLS, where it plays a role in the assembly of BASEMENT MEMBRANE as well as in cell migration, and may regulate the functions of other integrins. Two alternatively spliced isoforms of the alpha subunit (INTEGRIN ALPHA3), are differentially expressed in different cell types.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
An integrin alpha subunit that is unique in that it does not contain an I domain, and its proteolytic cleavage site is near the middle of the extracellular portion of the polypeptide rather than close to the membrane as in other integrin alpha subunits.
Proteins prepared by recombinant DNA technology.
An integrin alpha subunit that primarily associates with INTEGRIN BETA1 or INTEGRIN BETA4 to form laminin-binding heterodimers. Integrin alpha6 has two alternatively spliced isoforms: integrin alpha6A and integrin alpha6B, which differ in their cytoplasmic domains and are regulated in a tissue-specific and developmental stage-specific manner.
An integrin found in FIBROBLASTS; PLATELETS; MONOCYTES, and LYMPHOCYTES. Integrin alpha5beta1 is the classical receptor for FIBRONECTIN, but it also functions as a receptor for LAMININ and several other EXTRACELLULAR MATRIX PROTEINS.
The sum of the weight of all the atoms in a molecule.
The portion of the crystalline lens surrounding the nucleus and bound anteriorly by the epithelium and posteriorly by the capsule. It contains lens fibers and amorphous, intercellular substance.
Integrin alpha4beta1 is a FIBRONECTIN and VCAM-1 receptor present on LYMPHOCYTES; MONOCYTES; EOSINOPHILS; NK CELLS and thymocytes. It is involved in both cell-cell and cell- EXTRACELLULAR MATRIX adhesion and plays a role in INFLAMMATION, hematopoietic cell homing and immune function, and has been implicated in skeletal MYOGENESIS; NEURAL CREST migration and proliferation, lymphocyte maturation and morphogenesis of the PLACENTA and HEART.
An interleukin-1 subtype that occurs as a membrane-bound pro-protein form that is cleaved by proteases to form a secreted mature form. Unlike INTERLEUKIN-1BETA both membrane-bound and secreted forms of interleukin-1alpha are biologically active.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
An integrin found on fibroblasts, platelets, endothelial and epithelial cells, and lymphocytes where it functions as a receptor for COLLAGEN and LAMININ. Although originally referred to as the collagen receptor, it is one of several receptors for collagen. Ligand binding to integrin alpha2beta1 triggers a cascade of intracellular signaling, including activation of p38 MAP kinase.
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
A change from planar to elliptic polarization when an initially plane-polarized light wave traverses an optically active medium. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A subclass of alpha-adrenergic receptors that mediate contraction of SMOOTH MUSCLE in a variety of tissues such as ARTERIOLES; VEINS; and the UTERUS. They are usually found on postsynaptic membranes and signal through GQ-G11 G-PROTEINS.
This integrin alpha subunit combines with INTEGRIN BETA1 to form a receptor (INTEGRIN ALPHA5BETA1) that binds FIBRONECTIN and LAMININ. It undergoes posttranslational cleavage into a heavy and a light chain that are connected by disulfide bonds.
Integrin alpha1beta1 functions as a receptor for LAMININ and COLLAGEN. It is widely expressed during development, but in the adult is the predominant laminin receptor (RECEPTORS, LAMININ) in mature SMOOTH MUSCLE CELLS, where it is important for maintenance of the differentiated phenotype of these cells. Integrin alpha1beta1 is also found in LYMPHOCYTES and microvascular endothelial cells, and may play a role in angiogenesis. In SCHWANN CELLS and neural crest cells, it is involved in cell migration. Integrin alpha1beta1 is also known as VLA-1 and CD49a-CD29.
Any of various enzymatically catalyzed post-translational modifications of PEPTIDES or PROTEINS in the cell of origin. These modifications include carboxylation; HYDROXYLATION; ACETYLATION; PHOSPHORYLATION; METHYLATION; GLYCOSYLATION; ubiquitination; oxidation; proteolysis; and crosslinking and result in changes in molecular weight and electrophoretic motility.
Cysteine proteinase found in many tissues. Hydrolyzes a variety of endogenous proteins including NEUROPEPTIDES; CYTOSKELETAL PROTEINS; proteins from SMOOTH MUSCLE; CARDIAC MUSCLE; liver; platelets; and erythrocytes. Two subclasses having high and low calcium sensitivity are known. Removes Z-discs and M-lines from myofibrils. Activates phosphorylase kinase and cyclic nucleotide-independent protein kinase. This enzyme was formerly listed as EC 3.4.22.4.
A subclass of alpha-adrenergic receptors found on both presynaptic and postsynaptic membranes where they signal through Gi-Go G-PROTEINS. While postsynaptic alpha-2 receptors play a traditional role in mediating the effects of ADRENERGIC AGONISTS, the subset of alpha-2 receptors found on presynaptic membranes signal the feedback inhibition of NEUROTRANSMITTER release.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
A cell surface receptor mediating cell adhesion to the EXTRACELLULAR MATRIX and to other cells via binding to LAMININ. It is involved in cell migration, embryonic development, leukocyte activation and tumor cell invasiveness. Integrin alpha6beta1 is the major laminin receptor on PLATELETS; LEUKOCYTES; and many EPITHELIAL CELLS, and ligand binding may activate a number of signal transduction pathways. Alternative splicing of the cytoplasmic domain of the alpha6 subunit (INTEGRIN ALPHA6) results in the formation of A and B isoforms of the heterodimer, which are expressed in a tissue-specific manner.
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
This intrgrin is a key component of HEMIDESMOSOMES and is required for their formation and maintenance in epithelial cells. Integrin alpha6beta4 is also found on thymocytes, fibroblasts, and Schwann cells, where it functions as a laminin receptor (RECEPTORS, LAMININ) and is involved in wound healing, cell migration, and tumor invasiveness.
An enzyme that oxidizes an aldehyde in the presence of NAD+ and water to an acid and NADH. This enzyme was formerly classified as EC 1.1.1.70.
The alpha subunits of integrin heterodimers (INTEGRINS), which mediate ligand specificity. There are approximately 18 different alpha chains, exhibiting great sequence diversity; several chains are also spliced into alternative isoforms. They possess a long extracellular portion (1200 amino acids) containing a MIDAS (metal ion-dependent adhesion site) motif, and seven 60-amino acid tandem repeats, the last 4 of which form EF HAND MOTIFS. The intracellular portion is short with the exception of INTEGRIN ALPHA4.
The family of true frogs of the order Anura. The family occurs worldwide except in Antarctica.
A family of transmembrane glycoproteins (MEMBRANE GLYCOPROTEINS) consisting of noncovalent heterodimers. They interact with a wide variety of ligands including EXTRACELLULAR MATRIX PROTEINS; COMPLEMENT, and other cells, while their intracellular domains interact with the CYTOSKELETON. The integrins consist of at least three identified families: the cytoadhesin receptors(RECEPTORS, CYTOADHESIN), the leukocyte adhesion receptors (RECEPTORS, LEUKOCYTE ADHESION), and the VERY LATE ANTIGEN RECEPTORS. Each family contains a common beta-subunit (INTEGRIN BETA CHAINS) combined with one or more distinct alpha-subunits (INTEGRIN ALPHA CHAINS). These receptors participate in cell-matrix and cell-cell adhesion in many physiologically important processes, including embryological development; HEMOSTASIS; THROMBOSIS; WOUND HEALING; immune and nonimmune defense mechanisms; and oncogenic transformation.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
A mass spectrometric technique that is used for the analysis of a wide range of biomolecules, such as glycoalkaloids, glycoproteins, polysaccharides, and peptides. Positive and negative fast atom bombardment spectra are recorded on a mass spectrometer fitted with an atom gun with xenon as the customary beam. The mass spectra obtained contain molecular weight recognition as well as sequence information.
An integrin alpha subunit that binds COLLAGEN and LAMININ though its I domain. It combines with INTEGRIN BETA1 to form the heterodimer INTEGRIN ALPHA1BETA1.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.
The chemical or biochemical addition of carbohydrate or glycosyl groups to other chemicals, especially peptides or proteins. Glycosyl transferases are used in this biochemical reaction.
Brain waves characterized by a relatively high voltage or amplitude and a frequency of 8-13 Hz. They constitute the majority of waves recorded by EEG registering the activity of the parietal and occipital lobes when the individual is awake, but relaxed with the eyes closed.
Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies.
The transparent anterior portion of the fibrous coat of the eye consisting of five layers: stratified squamous CORNEAL EPITHELIUM; BOWMAN MEMBRANE; CORNEAL STROMA; DESCEMET MEMBRANE; and mesenchymal CORNEAL ENDOTHELIUM. It serves as the first refracting medium of the eye. It is structurally continuous with the SCLERA, avascular, receiving its nourishment by permeation through spaces between the lamellae, and is innervated by the ophthalmic division of the TRIGEMINAL NERVE via the ciliary nerves and those of the surrounding conjunctiva which together form plexuses. (Cline et al., Dictionary of Visual Science, 4th ed)
An integrin alpha subunit that occurs as alternatively spliced isoforms. The isoforms are differentially expressed in specific cell types and at specific developmental stages. Integrin alpha3 combines with INTEGRIN BETA1 to form INTEGRIN ALPHA3BETA1 which is a heterodimer found primarily in epithelial cells.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
Deficiency of the protease inhibitor ALPHA 1-ANTITRYPSIN that manifests primarily as PULMONARY EMPHYSEMA and LIVER CIRRHOSIS.
One of the two major classes of cholinergic receptors. Nicotinic receptors were originally distinguished by their preference for NICOTINE over MUSCARINE. They are generally divided into muscle-type and neuronal-type (previously ganglionic) based on pharmacology, and subunit composition of the receptors.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Drugs that selectively bind to and activate alpha adrenergic receptors.
Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Measurement of the intensity and quality of fluorescence.
A nuclear transcription factor. Heterodimerization with RETINOID X RECEPTOR GAMMA is important to metabolism of LIPIDS. It is the target of FIBRATES to control HYPERLIPIDEMIAS.
The most diversified of all fish orders and the largest vertebrate order. It includes many of the commonly known fish such as porgies, croakers, sunfishes, dolphin fish, mackerels, TUNA, etc.
A naturally occurring prostaglandin that has oxytocic, luteolytic, and abortifacient activities. Due to its vasocontractile properties, the compound has a variety of other biological actions.
Electrophoresis in which a pH gradient is established in a gel medium and proteins migrate until they reach the site (or focus) at which the pH is equal to their isoelectric point.
Drugs that bind to but do not activate alpha-adrenergic receptors thereby blocking the actions of endogenous or exogenous adrenergic agonists. Adrenergic alpha-antagonists are used in the treatment of hypertension, vasospasm, peripheral vascular disease, shock, and pheochromocytoma.
Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.
A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. The technique involves formation of an antigen-antibody complex which is labeled with fluorescein-conjugated anti-immunoglobulin antibody. (From Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)
The systematic study of the complete complement of proteins (PROTEOME) of organisms.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
A multistage process that includes the determination of a sequence (protein, carbohydrate, etc.), its fragmentation and analysis, and the interpretation of the resulting sequence information.
The continent lying around the South Pole and the southern waters of the Atlantic, Pacific, and Indian Oceans. It includes the Falkland Islands Dependencies. (From Webster's New Geographical Dictionary, 1988, p55)
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
Hepatocyte nuclear factor 1-alpha is a transcription factor found in the LIVER; PANCREAS; and KIDNEY that regulates HOMEOSTASIS of GLUCOSE.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.

Alpha-crystallin binds to the aggregation-prone molten-globule state of alkaline protease: implications for preventing irreversible thermal denaturation. (1/156)

Alpha-crystallin, the major eye-lens protein with sequence homology with heat-shock proteins (HSPs), acts like a molecular chaperone by suppressing the aggregation of damaged crystallins and proteins. To gain more insight into its chaperoning ability, we used a protease as the model system that is known to require a propeptide (intramolecular chaperone) for its proper folding. The protease ("N" state) from Conidiobolus macrosporus (NCIM 1298) unfolds at pH 2.0 ("U" state) through a partially unfolded "I" state at pH 3.5 that undergoes transition to a molten globule-(MG) like "I(A)" state in the presence of 0.5 M sodium sulfate. The thermally-stressed I(A) state showed complete loss of structure and was prone to aggregation. Alpha-crystallin was able to bind to this state and suppress its aggregation, thereby preventing irreversible denaturation of the enzyme. The alpha-crystallin-bound I(A) state exhibited native-like secondary and tertiary structure showing the interaction of alpha-crystallin with the MG state of the protease. 8-Anilinonaphthalene sulphonate (ANS) binding studies revealed the involvement of hydrophobic interactions in the formation of the complex of alpha-crystallin and protease. Refolding of acid-denatured protease by dilution to pH 7.5 resulted in aggregation of the protein. Unfolding of the protease in the presence of alpha-crystallin and its subsequent refolding resulted in the generation of a near-native intermediate with partial secondary and tertiary structure. Our studies represent the first report of involvement of a molecular chaperone-like alpha-crystallin in the unfolding and refolding of a protease. Alpha-crystallin blocks the unfavorable pathways that lead to irreversible denaturation of the alkaline protease and keeps it in a near-native, folding-competent intermediate state.  (+info)

Mass measurements of C-terminally truncated alpha-crystallins from two-dimensional gels identify Lp82 as a major endopeptidase in rat lens. (2/156)

Molecular chaperone activity of lens alpha-crystallins is reduced by loss of the C terminus. The purpose of this experiment was to 1) determine the cleavage sites produced in vitro by ubiquitous m-calpain and lens-specific Lp82 on alpha-crystallins, 2) identify alpha-crystallin cleavage sites produced in vivo during maturation and cataract formation in rat lens, and 3) estimate the relative activities of Lp82 and m-calpain by appearance of protease-specific cleavage products in vivo. Total soluble protein from young rat lens was incubated with recombinant m-calpain or Lp82 and 2 mM Ca2+. Resulting fragmented alpha-crystallins were separated by two-dimensional gel electrophoresis. Eluted alpha-crystallin spots were analyzed by mass spectrometry. Cleavage sites on insoluble alpha-crystallins were determined similarly in mature rat lens nucleus and in cataractous rat lens nucleus induced by selenite. In vitro proteolysis of alphaA-crystallin by Lp82 and m-calpain produced unique cleavage sites by removing 5 and 11 residues, respectively, from the C terminus. In vivo, the protease-specific truncations removing 5 and 11 residues from alphaA were both found in maturing lens, whereas only the truncation removing 5 residues was found in cataractous lens. Other truncation sites, common to both calpain isoforms, resulted from the removal of 8, 10, 16, 17, and 22 residues from the C terminus of alphaA. Using uniquely truncated alphaA-crystallins as in vivo markers, Lp82 and m-calpain were both found to be active during normal maturation of rat lens, whereas Lp82 seemed especially active during selenite cataract formation. These C-terminal truncations decrease chaperone activity of alpha-crystallins, possibly leading to the observed increases in insoluble proteins during aging and cataract. The methodology that allowed accurate mass measurements of proteins eluted from 2D gels should be useful to examine rapidly other post-translational modifications.  (+info)

Subunit exchange, conformational stability, and chaperone-like function of the small heat shock protein 16.5 from Methanococcus jannaschii. (3/156)

Hsp16.5, isolated from the hyperthermophilic Archaea Methanococcus jannaschii, is a member of the small heat-shock protein family. Small Hsps have 12- to 42-kDa subunit sizes and have sequences that are conserved among all organisms. The recently determined crystal structure of Hsp16.5 indicates that it consists discretely of 24 identical subunits. Using fluorescence resonance energy transfer, we show that at temperatures above 60 degrees C, the subunits of MjHsp16.5 freely and reversibly exchange with a rate constant of exchange at 68 degrees C of 0.067 min(-1). The subunit exchange reactions were strongly temperature-dependent, similar to the exchange reactions of the alpha-crystallins. The exchange reaction was specific to MjHsp16.5 subunits, as other sHsps such as alpha-crystallin were not structurally compatible and could not integrate into the MjHsp16.5 oligomer. In addition, we demonstrate that at temperatures as high as 70 degrees C, MjHsp16.5 retains its multimeric structure and subunit organization. Using insulin and alpha-lactalbumin as model target proteins, we also show that MjHsp16.5 at 37 degrees C is a markedly inefficient chaperone compared with other sHsps with these substrates. The results of this study support the hypothesis that MjHsp16.5 has a dynamic quaternary structure at temperatures that are physiologically relevant to M. jannaschii.  (+info)

Molecular adaptations of neuromuscular disease-associated proteins in response to eccentric exercise in human skeletal muscle. (4/156)

The molecular events by which eccentric muscle contractions induce muscle damage and remodelling remain largely unknown. We assessed whether eccentric exercise modulates the expression of proteinases (calpains 1, 2 and 3, proteasome, cathepsin B+L), muscle structural proteins (alpha-sarcoglycan and desmin), and the expression of the heat shock proteins Hsp27 and alphaB-crystallin. Vastus lateralis muscle biopsies from twelve healthy male volunteers were obtained before, immediately after, and 1 and 14 days after a 30 min downhill treadmill running exercise. Eccentric exercise induced muscle damage as evidenced by the analysis of muscle pain and weakness, creatine kinase serum activity, myoglobinaemia and ultrastructural analysis of muscle biopsies. The calpain 3 mRNA level was decreased immediately after exercise whereas calpain 2 mRNA level was increased at day 1. Both mRNA levels returned to control values by day 14. By contrast, cathepsin B+L and proteasome enzyme activities were increased at day 14. The alpha-sarcoglycan protein level was decreased immediately after exercise and at day 1, whereas the desmin level peaked at day 14. alphaB-crystallin and Hsp27 protein levels were increased at days 1 and 14. Our results suggest that the differential expression of calpain 2 and 3 mRNA levels may be important in the process of exercise-induced muscle damage, whereas expression of alpha-sarcoglycan, desmin, alphaB-crystallin and Hsp27 may be essentially involved in the subsequent remodelling of myofibrillar structure. This remodelling response may limit the extent of muscle damage upon a subsequent mechanical stress.  (+info)

Enzyme activity after resealing within ghost erythrocyte cells, and protection by alpha-crystallin against fructose-induced inactivation. (5/156)

The role of alpha-crystallin as a molecular chaperone has been shown in many in vitro studies. In the present paper, we report on the chaperone function of alpha-crystallin within resealed erythrocyte ghosts. Eight enzymes were individually resealed within erythrocyte ghosts and assayed at zero time and at 24 h. The ghost cell suspension was separated into soluble and membrane fractions. Five of the enzymes had significantly greater enzyme activity after 24 h than the control within the soluble fractions. Fructation caused a decrease in enzyme activity (relative to the control). Resealing of alpha-crystallin within the ghost cell alongside the enzymes protected against inactivation by fructose within the soluble fraction.  (+info)

Role of the C-terminal extensions of alpha-crystallins. Swapping the C-terminal extension of alpha-crystallin to alphaB-crystallin results in enhanced chaperone activity. (6/156)

Several small heat shock proteins contain a well conserved alpha-crystallin domain, flanked by an N-terminal domain and a C-terminal extension, both of which vary in length and sequence. The structural and functional role of the C-terminal extension of small heat shock proteins, particularly of alphaA- and alphaB-crystallins, is not well understood. We have swapped the C-terminal extensions between alphaA- and alphaB-crystallins and generated two novel chimeric proteins, alphaABc and alphaBAc. We have investigated the domain-swapped chimeras for structural and functional alterations. We have used thermal and non-thermal models of protein aggregation and found that the chimeric alphaB with the C-terminal extension of alphaA-crystallin, alphaBAc, exhibits dramatically enhanced chaperone-like activity. Interestingly, however, the chimeric alphaA with the C-terminal extension of alphaB-crystallin, alphaABc, has almost lost its activity. Pyrene solubilization and bis-1-anilino-8-naphthalenesulfonate binding studies show that alphaBAc exhibits more solvent-exposed hydrophobic pockets than alphaA, alphaB, or alphaABc. Significant tertiary structural changes are revealed by tryptophan fluorescence and near-UV CD studies upon swapping the C-terminal extensions. The far-UV CD spectrum of alphaBAc differs from that of alphaB-crystallin whereas that of alphaABc overlaps with that of alphaA-crystallin. Gel filtration chromatography shows alteration in the size of the proteins upon swapping the C-terminal extensions. Our study demonstrates that the unstructured C-terminal extensions play a crucial role in the structure and chaperone activity, in addition to generally believed electrostatic "solubilizer" function.  (+info)

Enhanced C-terminal truncation of alphaA- and alphaB-crystallins in diabetic lenses. (7/156)

PURPOSE: To investigate the influence of diabetes on the cleavage of C-terminal amino acid residues of alphaA- and alphaB-crystallins in human and rat lenses. METHODS: The human lenses were diabetic or age-matched control lenses from donors 57, 59, 69, and 72 years of age. Lenses were also obtained from streptozotocin-induced diabetic rats. Individual lens crystallins in water-soluble fractions were separated by gel-permeation chromatography. The high (alphaH)- and low (alphaL)-molecular-weight fractions were analyzed by electrospray ionization mass spectrometry. RESULTS: A typical mass spectrum of alphaA-crystallin from human lenses showed intact unmodified alphaA-crystallin, truncated alphaA(1-172), and monophosphorylated alphaA-crystallin. Diabetic lenses showed nearly twofold higher levels of alphaA(1-172) than did the control lenses. Also, the alphaH fraction consistently showed significantly higher levels of alphaA(1-172) than the alphaL fraction. Human alphaB-crystallin showed no evidence of C-terminal truncation. Rat alphaA-crystallin had five C-terminal-truncated components, most of which showed substantial increases in diabetes. Truncated alphaA(1-162) appeared only in the diabetic rat lenses, suggesting specific activation of m-calpain in diabetes. alphaB-crystallin had only one C-terminal-truncated component, alphaB(1-170), which also showed increased levels in diabetes. CONCLUSIONS: These data suggest that diabetic stress causes either enzymatic or nonenzymatic cleavage of peptide bonds between specific C-terminal amino acid residues. Such truncated alpha-crystallins appear to contribute to an increased level of the alphaH fraction generally present in diabetic lenses. Loss of alphaA-crystallin chaperone activity seems to be related to truncation of the C-terminal amino acid residues.  (+info)

Small heat-shock proteins regulate membrane lipid polymorphism. (8/156)

Thermal stress in living cells produces multiple changes that ultimately affect membrane structure and function. We report that two members of the family of small heat-shock proteins (sHsp) (alpha-crystallin and Synechocystis HSP17) have stabilizing effects on model membranes formed of synthetic and cyanobacterial lipids. In anionic membranes of dimyristoylphosphatidylglycerol and dimyristoylphosphatidylserine, both HSP17 and alpha-crystallin strongly stabilize the liquid-crystalline state. Evidence from infrared spectroscopy indicates that lipid/sHsp interactions are mediated by the polar headgroup region and that the proteins strongly affect the hydrophobic core. In membranes composed of the nonbilayer lipid dielaidoylphosphatidylethanolamine, both HSP17 and alpha-crystallin inhibit the formation of inverted hexagonal structure and stabilize the bilayer liquid-crystalline state, suggesting that sHsps can modulate membrane lipid polymorphism. In membranes composed of monogalactosyldiacylglycerol and phosphatidylglycerol (both enriched with unsaturated fatty acids) isolated from Synechocystis thylakoids, HSP17 and alpha-crystallin increase the molecular order in the fluid-like state. The data show that the nature of sHsp/membrane interactions depends on the lipid composition and extent of lipid unsaturation, and that sHsps can regulate membrane fluidity. We infer from these results that the association between sHsps and membranes may constitute a general mechanism that preserves membrane integrity during thermal fluctuations.  (+info)

Purpose: : In our previous studies, we have demonstrated that alpha-crystallins can negatively regulate stress-induced apoptosis through suppression of the ERK-mediated pathway and activation of the AKT signaling pathway. In the present study, we present evidence to show that alpha-crystallins can regulate the ATR-p53 pathway to prevent UVA-induced apoptosis. Methods: : UVA was used to irradiate human lens epithelial cells stably expressing vector, alphaA, and alphaB. Western blot analysis was used for detection of ATR, CHK1/2 and p53 activation. Reporter gene activity assay was used to explore the transactivity of p53. Hoechst staining was used for apoptosis assay. Results: : Human lens epithelial cells expressing either alphaA- or alphaB-crystallin are substantially resistant to UVA-induced apoptosis. UVA-induces activation of ATR and CHK1/2 kinases to activate p53 in vector-transfected cells. However, in alphaA- or alphaB-crystallin-transfected cells, activation of ATR, CHK1/2 kinases and p53 ...
Human alpha-crystallins were separated from fetal, young, senile nondiabetic and diabetic lenses. The effects of aging and diabetes mellitus were studied by fluorescence measurements, including emission maximum, quantum yield and polarization, using both intrinsic probes (tryptophan and non-tryptophan) and extrinsic probes [4-(N-iodoacetoxy)N-methylamino-7-nitrobenz-2-oxa-1,3-diazole (IANBD) and 6-(p-toluidinyl)naphthalene-2-sulfonate (TNS)]. Results indicate that diabetic effects (glycation and aggregation) give fluorescence change to a far greater extent than that of aging. This was demonstrated by a large decrease in tryptophan quantum yield and an increase in non-tryptophan quantum yield, and also by a decrease in polarization of non-tryptophan. The sulfhydryl (SH)-specific probe IANBD shows a blue-shift in emission maximum, a decrease in intensity and an increase in polarization. The hydrophobic probe TNS shows a decrease in both intensity and polarization. These results suggest that tryptophan
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Description: The heat-shock proteins (HSPs) belong to a larger group of polypeptides, the stress proteins, that are induced in various combinations in response to environmental challenges and developmental transitions. Synthesis of the small (27-kD) HSP has been shown to be correlated with the acquisition of thermotolerance. The deduced 199-amino acid HSP27 protein shows sequence similarity to mammalian alpha-crystallins. Approximately 20% of its residues are susceptible to phosphorylation. The HSP27 gene, which is mapped to 7q11.23 and has 3 exons1, produced a 2.2-kb transcript in an in vitro transcription assay. Decreasing ROS in cells expressing mutant huntingtin, HSP27 protects cells against oxidative stress2. In other words, HSP27 is a suppressor of polyglutamine (polyQ)-mediated cell death3. Furthermore, MAPKAPK5 is a major stress-activated kinase that can phosphorylate HSP27 in vitro.. Primary Antibody. ...
The chaperone-like protein alpha-crystallin is a approximately 35 subunit hetero-oligomer consisting of alphaA and alphaB subunits in a 3:1 molar ratio and has the function of maintaining eye lens transparency. We studied the thermal denaturation of alpha-crystallin by differential scanning calorimetry (DSC), circular dichroism (CD), and dynamic light scattering (DLS) as a function of pH. Our results show that between pH 7 and 10 the protein undergoes a reversible thermal transition. However, the thermodynamic parameters obtained by DSC are inconsistent with the complete denaturation of an oligomeric protein of the size of alpha-crystallin. Accordingly, the CD data suggest the presence of extensive residual secondary structure above the transition temperature. Within the pH range from 4 to 7 the increased aggregation propensity around the isoelectric point (pI approximately 6) precludes observation of a thermal transition. As pH decreases below 4 the protein undergoes a substantial unfolding. ...
Results. Effect of [alpha]-crystallin on restriction enzyme digestion. To determine whether the partially purified [alpha]-crystallin had inhibitory effects on restriction digestion of DNA, we assayed several commonly used restriction enzymes for activity in the presence of [alpha]-crystallin. The commonly used restriction enzymes, Bam HI, Hind III, Nde I, Pst I and Sst I all remained active in the presence of [alpha]-crystallin. For this experiment, a 5 fold excess of each enzyme (5 units) was incubated for 1 hour with 1 µg of plasmid DNA using manufacturers supplied buffer. Each reaction also contained 1 µl of the purified [alpha]-crystallin (10 mg/ml), final concentration, 1 mg/ml. Each selected enzyme digested the plasmid to completion indicating that [alpha]-crystallin had no major detrimental effects on restriction enzyme digestion (data not shown).. To assay the ability of [alpha]-crystallin to protect restriction enzymes from heat inactivation, we chose the enzyme Nde I. Unlike the ...
Ample evidence suggests that oxidative stress and other external stressors contribute to retinal and retinal pigment epithelium (RPE) pathology, as implicated in diseases like age-related macular degeneration. Therefore the understanding of cellular protection against these insults is of therapeutic importance. We adapted two approaches for studying the mechanisms of macular and retinal degeneration. In one, the contribution and significance of these pathologic processes was investigated by use of cultured human and mouse RPE. In the in vivo counterpart studies, we assessed the importance of alpha-crystallins in the retina (and RPE) in models using knockout mice and cobalt chloride injections. In addition to alpha-crystallins, we looked at other oxidative stress protectants of the cell and examined the redox regulation and antioxidant functions mediated by hepatocyte growth factor (HGF). These studies have greatly contributed to the elucidation of the pathways involved in retinal dysfunction and ...
A method for expressing proteins as a fusion chimera with a domain of p26 or alpha crystallin type proteins to improve the protein stability and solubility when over expressed in bacteria such as E. coli is provided. Genes of interest are cloned into the multiple cloning site of the Vector System just downstream of the p26 or alpha crystallin type protein and a thrombin cleavage site. Protein expression is driven by a strong bacterial promoter (TAC). The expression is induced by the addition of 1 mM IPTG that overcomes the lac repression (lac Iq). The soluble recombinant protein is purified using a fusion tag.
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Contributes to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions.
Charcot-Marie-Tooth disease (CMT) is the most common inherited neuromuscular disease and is characterized by considerable clinical and genetic heterogeneity. We previously reported a Russian family with autosomal dominant axonal CMT and assigned the locus underlying the disease (CMT2F; OMIM 606595) to chromosome 7q11-q21 (ref. 2). Here we report a missense mutation in the gene encoding 27-kDa small heat-shock protein B1 (HSPB1, also called HSP27) that segregates in the family with CMT2F. Screening for mutations in HSPB1 in 301 individuals with CMT and 115 individuals with distal hereditary motor neuropathies (distal HMNs) confirmed the previously observed mutation and identified four additional missense mutations. We observed the additional HSPB1 mutations in four families with distal HMN and in one individual with CMT neuropathy. Four mutations are located in the Hsp20-alpha-crystallin domain, and one mutation is in the C-terminal part of the HSP27 protein. Neuronal cells transfected with mutated HSPB1
Patagonian toothfish reaches sexual maturity late and grows slowly. This makes the species vulnerable to fishing pressure. In the 80s this species was discovered and the demand grew fast. in the 90s the species was strongly overfished. The state of the toothfish stocks was unknown for a long time, but since 2010 there are stock assessments being done for more and more stocks. The state of these stocks seems to improve.. This fishery with bottom trawls has a moderate amount of bycatch, but vulnerable shark species can be included in the bycatch. The fishery is managed by the CCAMLR (Commission on the Conservation of Antarctic Marine Living Resources). Several areas are closed for fishery and there are measures taken to reduce bycatch. The management is partly effective.. Illegal fishery is a big problem with the fishery on toothfish. ...
Crystallins are water-soluble proteins that compose about ninety% in the protein inside the lens.[13] The three main crystallin sorts present in the human eye are α-, β-, and γ-crystallins. Crystallins have a tendency to variety soluble, high-molecular pounds aggregates that pack tightly in lens fibers, Therefore raising the index of refraction of the lens while protecting its transparency. β and γ crystallins are found mostly during the lens, while subunits of α -crystallin happen to be isolated from other areas of the eye and the human body ...
Contributes to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions (By similarity).
Ed to contribute to its chaperone-like activity , while the N-terminal domains contain phosphorylation sites that are the targets of various protein kinases .
Alpha-crystallin B chain is a protein that in humans is encoded by the CRYAB gene. It is part of the small heat shock protein family and functions as molecular chaperone that primarily binds misfolded proteins to prevent protein aggregation, as well as inhibit apoptosis and contribute to intracellular architecture. Post-translational modifications decrease the ability to chaperone. Defects in this gene/protein have been associated with cancer and neurodegenerative diseases such as Alzheimers disease and Parkinsons disease. Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and ...
We report on a new cDNA clone (Qshsp10.4-CI) of a Quercus suber L. class-CI small heat-shock protein (sHsp) obtained from cork (phellem), a highly oxidatively stressed plant tissue. The deduced gene product lacks the C-terminal extension and the consensus I region of the alpha-crystallin domain, bei …
DNAJC4 (DnaJ heat shock protein family (Hsp40) member C4), Authors: Dessen P. Published in: Atlas Genet Cytogenet Oncol Haematol.
DNAJC21 (DnaJ heat shock protein family (Hsp40) member C21), Authors: Dessen P. Published in: Atlas Genet Cytogenet Oncol Haematol.
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View mouse Hspb11 Chr4:107253593-107279938 with: phenotypes, sequences, polymorphisms, proteins, references, function, expression
Complete information for HSPB6 gene (Protein Coding), Heat Shock Protein Family B (Small) Member 6, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Complete information for HSPB1P2 gene (Pseudogene), Heat Shock Protein Family B (Small) Member 1 Pseudogene 2, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
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In Leuconostoc oenos, different stresses such as heat, ethanol, and acid shocks dramatically induce the expression of an 18-kDa small heat shock protein called Lo 18. The corresponding gene (hsp18) was cloned from a genomic library of L. oenos constructed in Escherichia coli. A 2.3-kb DNA fragment carrying the hsp18 gene was sequenced. The hsp18 gene encodes a polypeptide of 148 amino acids with a calculated molecular mass of 16,938 Da. The Lo18 protein has a significant identity with small heat shock proteins of the alpha-crystallin family. The transcriptional start site was determined by primer extension. This experiment allowed us to identify the promoter region exhibiting high similarity to consensus promoter sequences of gram-positive bacteria, as well as E. coli. Northern blot analysis showed that hsp18 consists of a unique transcription unit of 0.6 kb. Moreover, hsp18 expression seemed to be controlled at the transcriptional level. This small heat shock protein was found to be ...
A quantitative analysis of cell division and cell elongation was carried out during lens morphogenesis in the rat. At 13 days of development elongating cells in the posterior part of the lens vesicle (presumptive fibre cells) have a lower mitotic activity than cells in the anterior vesicle. By 14 days these elongating cells do not divide. Thus at 14 days of development the lens can be separated into two compartments; a proliferation compartment in the anterior lens and an elongation compartment in the posterior lens.. The three main groups of lens-specific proteins, α-,β- and γ-crystallins, were localized by immunofiuorescence. Alpha-crystallin is the first crystallin to be detected and is localized in some lens pit cells at 12 days of development. By 14 days all lens cells contain α-crystallin. Beta- and β-crystallins are detected later at 12½ days and are localized in some cells situated primarily in the posterior part of the lens vesicle. At later stages of development these crystallins ...
Helianthus annuus hsp17.6 G1 protein: a small heat-shock protein from sunflower; amino acid sequence in first source; GenBank Z95153
Gene target information for HSPA13 - heat shock protein family A (Hsp70) member 13 (human). Find diseases associated with this biological target and compounds tested against it in bioassay experiments.
Chen X, Lin S, Liu Q, Huang J, Zhang W, Lin J, Wang Y, Ke Y, He H. Expression and interaction of small heat shock proteins (sHsps) in rice in response to heat stress. Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics, 2014, 1844 (4): 818-828. ...
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The Chilean Patagonian Toothfish fishery is a managed by the Chilean Government. For more information and a pdf version of the fact sheet, click the image below. COLTO Chile Fact Sheet 2021
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Hsp27, a small heat-shock protein, has important roles in many cellular processes, including cytoskeleton dynamics, cell differentiation, and apoptosis. Its expression in normal epidermis correlates with differentiation; however, little is known about the regulatory mechanisms involved. In this study, we report that Hsp27 undergoes upregulation, phosphorylation, and redistribution to the cytoskeleton during the late phase of epidermal keratinocyte differentiation. Our results also show that the expression of the dual leucine zipper-bearing kinase (DLK), an upstream activator of the MAP kinase pathways, is sufficient by itself to induce Hsp27 phosphorylation, cell periphery localization, and redistribution to the insoluble protein fraction (cytoskeleton) in poorly differentiated keratinocytes. This redistribution correlates with the insolubilization of cornified envelope-associated proteins such as involucrin. Interestingly, the effects of DLK on Hsp27 were blocked by PD98059, a selective ...
α-Crystallin, a member of small heat shock protein (sHsp) family, is comprised of αA and αB subunits and acts as a molecular chaperone by interacting with unfolding proteins to prevent their aggregation. The αA-crystallin homopolymer consists of 30-40 subunits that are undergoing dynamic exchange. α-Crystallin and αA-crystallin are poorer chaperones in the presence of the crowding agent, dextran. Using fluorescence resonance energy transfer, it is shown that the αAcrystallin subunit exchange rate strongly increased with temperature. Binding of reduced ovotransferrin to αA-crystallin markedly decreases the rate of subunit exchange, as does the presence of dextran. In addition, in the presence of dextran the effect of reduced ovotransferrin on decreasing the rate of subunit exchange of αA-crystallin is stronger than in the absence of dextran. Under the conditions of molecular crowding, the αA-crystallin subunit exchange rate is not temperature-dependent. The exchange rate of αA-crystallin
Effect of long term heat stress and dietary restriction on the expression of small heat shock protein sHSP genes in rat liver tissue ...
Under changes in conditions as diverse as temperature, oxidation or pH, proteins in an organism may undergo harmful denaturation. Small Heat Shock proteins act as paramedics of the cell: during such events they quicky intervene by binding nascently unfolding proteins, leading them to refolding or denaturation pathways. Small heat shock proteins are ubiquitous in all kingdoms of life, but especially effective in plants: after all, plants cannot escape from harsh environmental conditions!. Collaborating with Benesch (University of Oxford) and Vierling (UMass) groups, we have contributed to shedding light into the mode of action of small Heat Shock Proteins in wheat and pea.. We describe a mechanism whereby dimers of these proteins are responsible for capturing their substrate, before assemblying into larger complexes. With our own integrative modelling methods using distance restraints and collision cross-section measurements, we demonstrate that small heat shock protein dimers assemble into ...
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Analytical data on determination of reproductive potential of Antarctic toothfish D. mawsoni in the Pacific (SSRUs 88.1, 88.2, 88.3), Indian Ocean (SSRUs 58.4.1 и 58.4.2) and Atlantic (SSRU 48.6, 48.5) Antarctic areas are presented. Morphological indices of females and males, indices of gonads, fecundity, and condition by Fulton are described. Spawning time and regions for Antarctic toothfish are discussing.. ...
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Abstract: Small heat shock proteins form large cytosolic assemblies from an α-crystallin domain (ACD) flanked by sequence extensions. Mutation of a conserved arginine in the ACD of several human small heat shock protein family members causes many common inherited diseases of the lens and neuromuscular system. The mutation R120G in αB-crystallin causes myopathy, cardiomyopathy and cataract. We have solved the X-ray structure of the excised ACD dimer of human αB R120G close to physiological pH and compared it with several recently determined wild-type vertebrate ACD dimer structures. Wild-type excised ACD dimers have a deep groove at the interface floored by a flat extended bottom sheet. Solid-state NMR studies of large assemblies of full-length αB-crystallin have shown that the groove is blocked in the ACD dimer by curvature of the bottom sheet. The crystal structure of R120G ACD dimer also reveals a closed groove, but here the bottom sheet is flat. Loss of Arg120 results in rearrangement of ...
article{28a73216-cb79-4da4-9c2e-f0213dfb2b2d, abstract = {During evolution of land plants, a specific motif occurred in the N-terminal domain of the chloroplast-localized small heat shock protein, Hsp21: a sequence with highly conserved methionines, which is predicted to form an amphipathic -helix with the methionines situated along one side. The functional role of these conserved methionines is not understood. We have found previously that treatment, which causes methionine sulfoxidation in Hsp21, also leads to structural changes and loss of chaperone-like activity. Here, mutants of Arabidopsis thaliana Hsp21 protein were created by site-directed mutagenesis, whereby conserved methionines were substituted by oxidation-resistant leucines. Mutants lacking the only cysteine in Hsp21 were also created. Protein analyses by nondenaturing electrophoresis, size exclusion chromatography, and circular dichroism proved that sulfoxidation of the four highly conserved methionines (M49, M52, M55, and M59) is ...
Heat shock proteins (HSPs) are ubiquitous in living organisms. HSPs are an essential component for cell growth and survival; the main function of HSPs is controlling the folding and unfolding process of proteins. According to molecular function and mass, HSPs are categorized into six different families: HSP20 (small HSPS), HSP40 (J-proteins), HSP60, HSP70, HSP90, and HSP100. In this paper, improved methods for HSP prediction are proposed—the split amino acid composition (SAAC), the dipeptide composition (DC), the conjoint triad feature (CTF), and the pseudoaverage chemical shift (PseACS) were selected to predict the HSPs with a support vector machine (SVM). In order to overcome the imbalance data classification problems, the syntactic minority oversampling technique (SMOTE) was used to balance the dataset. The overall accuracy was 99.72% with a balanced dataset in the jackknife test by using the optimized combination feature SAAC+DC+CTF+PseACS, which was 4.81% higher than the
Dr. Mason Posner has students use molecular biology techniques to understand how eye lens proteins adapt to changes in environmental temperature.. We are currently investigating the evolution and biological role of lens proteins called crystallins. These proteins are responsible for making the lens transparent and refracting light so that focused images fall on the retina. Amazingly, one family of crystallins, the alpha crystallins, also protect other proteins from the harmful effects of aging that can lead to lens cataracts, one of the leading causes of blindness in humans. Alpha crystallins are also involved in the original development of the lens in vertebrate embryos, and they have been linked to many diseases of the nervous system, heart, skeletal muscle, and are now known to be involved in many cancers.. Most research into alpha crystallins is done with mammals. However, by studying how this protein has evolved in a number of fish species that live at different environmental temperatures, ...
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Purpose: : A previous microarray study from our laboratory demonstrated that in lens epithelial cells treated with quercetin (10 µM), 65% of genes that showed significant increased expression (p≤0.01) were regulated by hypoxia inducible factor-1 (HIF-1). We have, therefore, investigated the effect of quercetin on the HIF-1 signalling pathway in human lens epithelial cells. Methods: : FHL-124 cells were grown to 90% confluency and exposed to quercetin (10 or 30 µM). Protein and mRNA levels were analyzed by Western blot and qRT-PCR respectively. Immunocytochemistry experiments were carried out for visualisation of HIF-1. VEGF was measured in cell medium using ELISA. Results: : Quercetin (30 µM) induced an increase in HIF-1 protein levels after 4 and 24 hr, with a greater than 50-fold increase compared to untreated cells at 4 hr. At 10 µM quercetin, increases were seen after 24 hr. Visualisation of HIF-1 within the cell showed that quercetin caused its translocation to the nucleus and this ...
Gene target information for Dnajb11 - DnaJ heat shock protein family (Hsp40) member B11 (house mouse). Find diseases associated with this biological target and compounds tested against it in bioassay experiments.
article{c39ed0c6-71b3-44bd-ae7d-1c63648fa85e, abstract = {,p,The small heat shock protein (sHsp) chaperones are crucial for cell survival and can prevent aggregation of client proteins that partially unfold under destabilizing conditions. Most investigations on the chaperone activity of sHsps are based on a limited set of thermosensitive model substrate client proteins since the endogenous targets are often not known. There is a high diversity among sHsps with a single conserved β-sandwich fold domain defining the family, the α-crystallin domain, whereas the N-terminal and C-terminal regions are highly variable in length and sequence among various sHsps and conserved only within orthologues. The endogenous targets are probably also varying among various sHsps, cellular compartments, cell type and organism. Here we have investigated Hsp21, a non-metazoan sHsp expressed in the chloroplasts in green plants which experience huge environmental fluctuations not least in temperature. We describe how ...
Human Lens Epithelial Cells (HLEpiC) from Creative Bioarray are isolated from the human lens. HLEpiC are cryopreserved at primary culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HLEpiC are characterized by immunofluorescent method with antibodies to cytokeratin-18, cytokeratin-19 and fibronectin. HLEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HLEpiC are guaranteed to further culture in the conditions provided by Creative Bioarray ...
The eleven students involved learned basic experimental design (with positive and negative controls), troubleshooting, a wide variety of laboratory techniques, and data collection, analysis and presentations skills. In addition, students learned to read and analyze scientific work from other labs since they are given scientific articles to read beginning on their first day in the lab. This project is in collaboration with Ivor Benjamin M.D., Ph.D. at the University of Utah, which allowed these students the unique opportunity of discussing their research with a larger audience once a month. In addition to intellectual enrichment, students learned to function as a team, with those more experienced (graduate students Kelsey Langston and Whitney Hayes) mentoring those who are new in the lab. As students progressed in their understanding of the project and mastery of basic laboratory skills, they become involved in planning our weekly group meeting, preparing figures for presentations/publications, ...
Ultrasonic and cryogenic instrumentation for the removal of unwanted tissue material from an animal, such as a human, and more particularly, adapted for the removal of cataracts, in surgical operations. The ultrasonic and cryogenic instrumentation include hand-held instruments with the cryogenic instrumentation adapted to first freeze the cataract lens and after the cataract lens has unfrozen the ultrasonic instrumentation is adapted by a vibrating tool to reduce the volume of the cataract and thereafter, through the simultaneous introduction of a fluid and creating a flow, the cataract mass exits through the incision around the vibrating tool until all of the unwanted tissue forming the cataract lens is removed.
The current status of research on the structure, regulation, mechanism and importance of Rubisco activase is reviewed. The activase is now recognized to be a member of the AAA(+) family, whose members participate in macromolecular complexes that perform diverse chaperone-like functions. The conserve …
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MP26 and alpha-crystallin are palmitoylated". FEBS Lett. 262 (2): 356-8. doi:10.1016/0014-5793(90)80228-B. PMID 2335219. S2CID ... Prabhakaram M, Katz ML, Ortwerth BJ (1997). "Glycation mediated crosslinking between alpha-crystallin and MP26 in intact lens ... 1991). "Lens protein gene expression: alpha-crystallins and MIP". Lens and Eye Toxicity Research. 8 (2-3): 319-44. PMID 1911643 ...
Bennardini F, Wrzosek A, Chiesi M (August 1992). "Alpha B-crystallin in cardiac tissue. Association with actin and desmin ... a conserved alpha helix rod, a variable non alpha helix head, and a carboxy-terminal tail.[12] Desmin, as all intermediate ... Desmin has been shown to interact with desmoplakin[14] and αB-crystallin.[15] ... filaments, shows no polarity when assembled.[12] The rod domain consists of 308 amino acids with parallel alpha helical coiled ...
Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. These ... Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is ... the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Alpha B chain crystallins (αBC) can be induced by heat shock, ... Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as ...
Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as ... Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four ... Beta-crystallin B3 is a protein that in humans is encoded by the CRYBB3 gene. Crystallins are separated into two classes: taxon ... Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none ...
Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as ... Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four ... Gamma-crystallin B is a protein that in humans is encoded by the CRYGB gene. Crystallins are separated into two classes: taxon- ... Four gamma-crystallin genes (gamma-A through gamma-D) and three pseudogenes (gamma-E, gamma-F, gamma-G) are tandemly organized ...
Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins ... Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is ... the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an ... Alpha-crystallin A chain is a protein that in humans is encoded by the CRYAA gene. Crystallins are separated into two classes: ...
Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as ... Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four ... Beta-crystallin B2 is a protein that in humans is encoded by the CRYBB2 gene. Crystallins are separated into two classes: taxon ... 2005). "Unfolding crystallins: The destabilizing role of a β-hairpin cysteine in BetaB2-crystallin by simulation and experiment ...
Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as ... Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four ... Gamma-crystallin S is a protein that in humans is encoded by the CRYGS gene. Crystallins are separated into two classes: taxon- ... "Entrez Gene: CRYGS crystallin, gamma S". Human CRYGS genome location and CRYGS gene details page in the UCSC Genome Browser. ...
Structurally, this family is characterised by the presence of a conserved C-terminal domain, alpha-crystallin domain, of about ... Merck KB, de Jong WW, Bloemendal H, Groenen PJ (1994). "Structure and modifications of the junior chaperone alpha-crystallin. ...
"Monitoring the prevention of amyloid fibril formation by alpha-crystallin. Temperature dependence and the nature of the ... Intra-cellular deposits of tau protein are also seen in the disease, and may also be implicated, as has aggregation of alpha ... "Beta-amyloid exhibits antagonistic effects on alpha 7 nicotinic acetylcholine receptors in orchestrated manner". Journal of ...
Kappe G, Franck E, Verschuure P, Boelens WC, Leunissen JA, de Jong WW (Jun 2003). "The human genome encodes 10 alpha-crystallin ... "Entrez Gene: HSPB6 heat shock protein, alpha-crystallin-related, B6". Kato K, Goto S, Inaguma Y, Hasegawa K, Morishita R, Asano ... "Purification and characterization of a 20-kDa protein that is highly homologous to alpha B crystallin". J. Biol. Chem. 269 (21 ... Fontaine JM, Sun X, Benndorf R, Welsh MJ (2005). "Interactions of HSP22 (HSPB8) with HSP20, alphaB-crystallin, and HSPB3". ...
... coassembly with alpha-crystallin but not with vimentin". Exp. Eye Res. 60 (2): 181-92. doi:10.1016/S0014-4835(95)80009-3. PMID ... "Human alpha A-crystallin missing N-terminal domain poorly complexes with filensin and phakinin". Biochemical and Biophysical ...
Shum WK, Maleknia SD, Downard KM (September 2005). "Onset of oxidative damage in alpha-crystallin by radical probe mass ... Downard KM, Kokabu Y, Ikeguchi M, Akashi S (November 2011). "Homology-modelled structure of the βB2B3-crystallin heterodimer ...
Characterization of lens alpha-crystallin tryptophan microenvironments by room temperature phosphorescence spectroscopy. ... The anaerobic photolysis of lens alpha-crystallin: evidence for triplet state mediated photodamage. Photochem Photobiol. 1990 ... Phosphorescence measurements of calf gamma-II, III, and IV crystallins at 77 and 293 K. Exp Eye Res. 1989 May;48(5):627-39. 35 ...
DeJong W.W.; Zweers A.; Goodman M. (1981). "Relationship of aardvark to elephants, hyraxes and sea cows from alpha-crystallin ... DeJong, W.W.; J.A.M. Leunissen & G.J. Wistow (1993). "Eye lens crystallins and the phylogeny of placental orders: evidence for ...
Van Montfort R, Slingsby C, Vierling E (2001). "Structure and function of the small heat shock protein/alpha-crystallin family ... "Copurification of small heat shock protein with alpha B crystallin from human skeletal muscle". The Journal of Biological ... Hsp27 is a chaperone of the sHsp (small heat shock protein) group among α-crystallin, Hsp20, and others. The common functions ... Hsp27 is rather unique among sHsps in that its α-crystallin domain contains a cysteine residue at its dimer interface, which ...
"Human alpha A-crystallin missing N-terminal domain poorly complexes with filensin and phakinin". Biochemical and Biophysical ...
Goenka S, Raman B, Ramakrishna T, Rao CM (2001). "Unfolding and refolding of a quinone oxidoreductase: alpha-crystallin, a ... The former class is also called phylogenetically-restricted crystallins. This gene encodes a taxon-specific crystallin protein ... "Entrez Gene: CRYZ crystallin, zeta (quinone reductase)". Human CRYZ genome location and CRYZ gene details page in the UCSC ... Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major ...
Bsibsi M, Holtman IR, Gerritsen WH, Eggen BJ, Boddeke E, van der Valk P, van Noort JM, Amor S (2013). "Alpha-B-Crystallin ...
Alpha crystallin (α4- crystallin) or hspb4 is involved in the development of lens in Zebrafish as it is expressed in response ... Expression of the hspb4 gene, which codes for alpha crystallin, increases considerably in the lens in response to heat shock. ... Hsp90, hsp84, hsp70, hsp27, hsp20, and alpha B crystallin all have been reported as having roles in the cardiovasculature. ... A conserved protein binding domain of approximately 80 amino-acid alpha crystallins are known as small heat shock proteins ( ...
... alpha-crystallin, with molten globule states of bovine alpha-lactalbumin.». J. Biol. Chem. 272 (44): 27722-9. DOI:10.1074/jbc. ... 1 2 Entrez Gene: LALBA lactalbumin, alpha-.. *↑ Hall L, Davies MS, Craig RK (January 1981). «The construction, identification ... Lactation is disrupted by alpha-lactalbumin deficiency and can be restored by human alpha-lactalbumin gene replacement in mice ... Hall L, Emery DC, Davies MS, et al. (1987). «Organization and sequence of the human alpha-lactalbumin gene.». Biochem. J. 242 ( ...
Bhat SP, Nagineni CN (Jan 1989). "alpha B subunit of lens-specific protein alpha-crystallin is present in other ocular and non- ... Wang K, Spector A (May 1994). "The chaperone activity of bovine alpha crystallin. Interaction with other lens crystallins in ... Ubiquitous α -crystallins and bird δ -crystallins are two examples. The α-crystallins, which contributed to the discovery of ... η-crystallin, α-enolase/τ-crystallin, and lactic dehydrogenase/ -crystallin. Also, the anuran corneal epithelium, which can ...
"Monitoring the prevention of amyloid fibril formation by alpha-crystallin. Temperature dependence and the nature of the ...
... and alpha B crystallin. Tau proteins may also be present, and Lewy bodies may occasionally be surrounded by neurofibrillary ... the primary structural component of which is alpha-synuclein. Cortical Lewy bodies are also composed of alpha-synuclein fibrils ... "Alpha-synuclein is a DNA binding protein that modulates DNA repair with implications for Lewy body disorders". Sci Rep. 2019 ... Alpha-synuclein modulates DNA repair processes, including repair of DNA double-strand breaks (DSBs) by the process of non- ...
... microcornea and corneal opacity and novel mutation in the alpha A crystallin gene (CRYAA)". Am. J. Med. Genet. A. 146 (7): 833- ...
Aggeli IK, Beis I, Gaitanaki C (Jul 2008). "Oxidative stress and calpain inhibition induce alpha B-crystallin phosphorylation ... "A Novel MAPK phosphatase MKP-7 acts preferentially on JNK/SAPK and p38 alpha and beta MAPKs". The Journal of Biological ... "Cardioprotective role of P38 MAPK during myocardial infarction via parallel activation of α-crystallin B and Nrf2". Journal of ... some reports demonstrated that p38 MAPK also involves in anti-apoptotic effect via phosphorylation of αβ-Crystallin or ...
"Nuclear speckle localisation of the small heat shock protein alpha B-crystallin and its inhibition by the R120G cardiomyopathy- ... SAFB has been shown to interact with: Estrogen receptor alpha, HNRPD, SAFB2, and TAF15. GRCh38: Ensembl release 89: ... "Structure-function analysis of the estrogen receptor alpha corepressor scaffold attachment factor-B1: identification of a ...
The cause is lens hardening by decreasing levels of alpha-crystallin, a process which may be sped up by higher temperatures. ...
The expression of chick alpha A2-crystallin RNA during lens development and transdifferentiation. Biol Cell. 1985;54(2):101-8. ... Crystallin gene expression was further explored in a detailed series of studies using long-term cell culture models in which ... Crystallin sequence similarities with, or in some cases their identity to particular enzymes or heat shock proteins led to the ... Beta B2-crystallin in the mammalian retina. Exp Eye Res. 1995 Oct;61(4):423-8. PubMed PMID 8549683. Wistow G, Piatigorsky J. ...
2002). "The NADPH:quinone oxidoreductase P1-zeta-crystallin in Arabidopsis catalyzes the alpha,beta-hydrogenation of 2-alkenals ... Other names in common use include NAD(P)H-dependent alkenal/one oxidoreductase, and NADPH:2-alkenal alpha,beta-hydrogenase. ...
... and alpha B crystallin. Tau proteins may also be present, and Lewy bodies may occasionally be surrounded by neurofibrillary ... the primary structural component of which is alpha-synuclein. Cortical Lewy bodies are also composed of alpha-synuclein fibrils ... A Lewy body is composed of the protein alpha-synuclein associated with other proteins, such as ubiquitin,[10] neurofilament ... Top panels show a 60-times magnification of the alpha-synuclein intraneuronal inclusions aggregated to form Lewy bodies. The ...
Alpha crystallin. *Clusterin. *Survival of motor neuron *SMN1. *SMN2. Protein targeting. *Signal peptide ...
C-terminal domain - rich in alpha helical structure acts as a 'lid' for the substrate binding domain. The helical subdomain ...
α-Amanatin, see Alpha-amanitin. *Aimínaigéad Amino acid. *Séaróideach anabalach Anabolic steroid ...
"Control of NF-kappa B transcriptional activation by signal induced proteolysis of I kappa B alpha". Philos. Trans. R. Soc. ...
... and phosphorylation dependent ubiquitinationof I kappa B alpha catalyzed by Ubc3 and Ubc4". Oncogene. 19 (31): 3529-36. doi: ...
Crystallin. *Alpha (A. *B). *Beta (A1. *A2. *A4. *B1. *B2. *B3). *Gamma (A ...
Chen D, Li M, Luo J, Gu W (April 2003). "Direct interactions between HIF-1 alpha and Mdm2 modulate p53 function". The Journal ... Bálint E, Bates S, Vousden KH (July 1999). "Mdm2 binds p73 alpha without targeting degradation". Oncogene. 18 (27): 3923-9. doi ...
COL11A1: collagen, type XI, alpha 1. *CPT2: carnitine palmitoyltransferase II. *CRYZ: Crystallin zeta ...
Alpha crystallin. *Clusterin. *Survival of motor neuron *SMN1. *SMN2. Protein targeting. *Signal peptide ...
MAN1A2 mannosidase alpha class 1A member 2 [ Homo sapiens (human) ]». Gene. NCBI. 6. juni 2017. Henta 26. juni 2017.. ... CRYZ crystallin zeta [ Homo sapiens (human) ]». Gene. NCBI. 4. juni 2017. Henta 26. juni 2017.. ... IFI6 interferon alpha inducible protein 6 [ Homo sapiens (human) ]». Gene. NCBI. 4. juni 2017. Henta 26. juni 2017.. ... COL11A1 collagen type XI alpha 1 chain [ Homo sapiens (human) ]». Gene. NCBI. 6. juni 2017. Henta 26. juni 2017.. ...
Also in the 1970s, physicists Tanaka & Benedek at MIT identified phase-separation behaviour of gamma-crystallin proteins from ... Robert Corey and Herman Branson correctly proposed the alpha helix and beta sheet as the primary structural motifs in protein ... solid phase transitions such as DNA condensation during prophase of the cell cycle or protein condensation of crystallins in ...
These compounds are thought to act as bidentate nucleophiles that attack the adjacent carbonyls in the alpha-dicarbonyl ... and crystallin of the eyes. Covalent protein cross-links irreversibly link proteins together in the ECM of tissues. Glucosepane ... such as collagen in the skin and crystallin in the eyes. Skin collagen, for instance, has a half-life of fifteen years. Because ...
3DG as well as AGEs play a role in the modification and cross-linking of long-lived proteins such as crystallin and collagen, ... Araki A (September 1997). "[Oxidative stress and diabetes mellitus: a possible role of alpha-dicarbonyl compounds in free ...
... and Asp477 forms hydrogen bonds with the alpha hydroxyl group on the substrate, where it works to effectively bind the ... in the mammalian cornea by the stromal keratocytes and epithelial cells and is reputed to be one of the corneal crystallins. ... important catalytic effects because of its orientation in the middle of the active site and its interactions with the alpha ...
As a component of alpha ring, proteasome subunit alpha type-3 contributes to the formation of heptameric alpha rings and ... Boelens WC, Croes Y, de Jong WW (January 2001). "Interaction between alphaB-crystallin and the human 20S proteasomal subunit C8 ... Proteasome subunit alpha type-3 also known as macropain subunit C8 and proteasome component C8 is a protein that in humans is ... A role of the proteasome subunit alpha type-3 has been linked in underlying mechanisms of human malignancies. It has been ...
... alpha-crystallin a chain MeSH D12.776.306.366.100.300 - alpha-crystallin b chain MeSH D12.776.306.366.300.100 - beta-crystallin ... alpha 1-antichymotrypsin MeSH D12.776.377.715.085.085 - alpha 1-antitrypsin MeSH D12.776.377.715.085.100 - alpha-macroglobulins ... steroid 12-alpha-hydroxylase MeSH D12.776.422.220.453.915.737 - steroid 16-alpha-hydroxylase MeSH D12.776.422.220.453.915.748 ... Retinoid X receptor alpha MeSH D12.776.826.701.500.625 - Retinoid X receptor beta MeSH D12.776.826.701.500.750 - Retinoid X ...
... and most surprisingly jellyfish crystallins, although these proteins appear to have lost the presumed active site residues. ADP ... acetyl-ADP-ribose and alpha-nicotinamide adenine dinucleotide as substrates. The family also includes ADP-ribosyl-(dinitrogen ...
To this end, a transgenic mouse line was created expressing porcine TGF-β1 under the lens-specific αA-crystallin promoter and ... In a mouse model of Alzheimer's plaque formation, IL-1-alpha effects an increase in perlecan expression in response to brain ... Perlecan levels were increased in the hippocampus but not in the striatum during the healing period, along with IL 1-alpha ... The effect was more pronounced in the βB-1 Crystallin promoter-driven line. The IL family of inflammatory cytokines also ...
This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and, thus, plays a role in ... Chen Q, Dowhan DH, Liang D, Moore DD, Overbeek PA (July 2002). "CREB-binding protein/p300 co-activation of crystallin gene ... "MRG1 binds to the LIM domain of Lhx2 and may function as a coactivator to stimulate glycoprotein hormone alpha-subunit gene ... "Structural basis for recruitment of CBP/p300 by hypoxia-inducible factor-1 alpha". Proc. Natl. Acad. Sci. U.S.A. 99 (8): 5367- ...
IPR002068 A-crystallin/Hsp20_dom. IPR001436 Alpha-crystallin/HSP. IPR012274 Alpha-crystallin_A. IPR003090 Alpha-crystallin_N. ... IPR002068 A-crystallin/Hsp20_dom. IPR001436 Alpha-crystallin/HSP. IPR012274 Alpha-crystallin_A. IPR003090 Alpha-crystallin_N. ... Crystallin alpha AImported. ,p>Information which has been imported from another database using automatic procedures.,/p> ,p>,a ... tr,B4Z7V1,B4Z7V1_9FALC Crystallin alpha A (Fragment) OS=Daptrius ater OX=56348 GN=CRYAA PE=3 SV=1 ...
Rabbit polyclonal Alpha B Crystallin antibody. Validated in WB, IHC and tested in Mouse, Human, Pig. Immunogen corresponding to ... Anti-Alpha B Crystallin antibody (ab231268) at 2 µg/ml + Recombinant human Alpha B Crystallin protein.. Predicted band size: 20 ... Anti-Alpha B Crystallin antibody. See all Alpha B Crystallin primary antibodies. ... Primary - Rabbit Anti-Alpha B Crystallin antibody (ab231268) WB, IHC-P Secondary - Goat Anti-Rabbit IgG H&L (HRP) (ab205718) ...
Rabbit polyclonal Alpha B Crystallin antibody. Validated in WB, IP, IHC, ICC and tested in Mouse, Rat, Chicken, Cow, Human, Pig ... Alpha B Crystallin was immunoprecipitated using 0.5mg Mouse Spinal Cord tissue, 5µg of Rabbit polyclonal to Alpha B Crystallin ... Anti-Alpha B Crystallin antibody. See all Alpha B Crystallin primary antibodies. ... Anti-Alpha B Crystallin antibody (ab13497) at 1/1000 dilution + Human spinal cord tissue lysate - total protein (ab29188) at 10 ...
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
IPR037882, ACD_alphaB-crystallin. IPR001436, Alpha-crystallin/sHSP_animal. IPR003090, Alpha-crystallin_N. IPR008978, HSP20- ... IPR037882, ACD_alphaB-crystallin. IPR001436, Alpha-crystallin/sHSP_animal. IPR003090, Alpha-crystallin_N. IPR008978, HSP20- ... "Site selectivity in the glycation of alpha A- and alpha B-crystallins by glucose.". Abraham E.C., Cherian M., Smith J.B.. ... "Site selectivity in the glycation of alpha A- and alpha B-crystallins by glucose.". Abraham E.C., Cherian M., Smith J.B.. ...
... alpha B), Authors: Aysegul Ilhan, Ludwig Wagner, Wolfgang Gartner. Published in: Atlas Genet Cytogenet Oncol Haematol. ... A-crystallin/Hsp20_dom Alpha-crystallin/HSP Alpha-crystallin_B Alpha-crystallin_N HSP20 HSP20-like_chaperone ... Crystallins are a highly conserved protein family consisting of three classes, alpha, beta and gamma. The alpha crystallins are ... Complete structure of the alpha B-crystallin gene: conservation of the exon-intron distribution in the two nonlinked alpha- ...
Alpha-Crystallins and Tumorigenesis. Author(s): P. Chen, W. Ji, F.-Y. Liu, H.-Z. Tang, S. Fu, X. Zhang, M. Liu, L. Gong, M. ... αA- and αB-crystallins, the major lens structure proteins and members of the small heat-shock proteins (sHSPs) family, play ... Abstract:αA- and αB-crystallins, the major lens structure proteins and members of the small heat-shock proteins (sHSPs) family ... In addition, recent studies have revealed that the abnormal expressions and functions of both α-crystallins are associated with ...
What is alpha crystallin B chain? Meaning of alpha crystallin B chain medical term. What does alpha crystallin B chain mean? ... Looking for online definition of alpha crystallin B chain in the Medical Dictionary? alpha crystallin B chain explanation free ... redirected from alpha crystallin B chain) CRYAB. A gene on chromosome 11q22.3-q23.1 that encodes alpha crystallin B, a protein ... Alpha crystallin B chain , definition of alpha crystallin B chain by Medical dictionary https://medical-dictionary. ...
Purified anti-Alpha Crystallin B - read details of BioLegend antibodies in the SelectScience.net Antibody products and ... Alpha-crystallin B chain, alpha B crystallin, heat shock protein beta-5, rosenthal fiber component, heat-shock 20 kD like- ... Purified anti-Alpha Crystallin B. Be the first to review this product ... Purified anti-Alpha Crystallin B; Clone: Poly9079; Reactivity: Human, Mouse; Apps: IHC, WB; Size: 200 μl ...
... Description: Alpha B crystallin is a lens protein which ... Alpha B crystallin is rarely found in neurofibrillary tangles. The role of Alpha B crystallin in these inclusion bodies is ... 1992) Accumulation of alpha B-crystallin in central nervous system glia and neurons in pathologic conditions. Am J Pathol. 140( ... 1991) Expression of alpha B-crystallin in the developing rat kidney. Kidney Int. 40(1):52-6. PubMed Abstract. ...
... alpha-Crystallin-Related, B6 ELISA Kits. Show all Heat Shock Protein, alpha-Crystallin-Related, B6 (HSPB6) ELISA Kits with ... alpha-Crystallin-Related, B6 Antikörper (120) und Heat Shock Protein, alpha-Crystallin-Related, B6 Proteine (14) und viele ... alpha-Crystallin-Related, B6 Interaktionspartner. Human Heat Shock Protein, alpha-Crystallin-Related, B6 (HSPB6) ... alpha-Crystallin-Related, B6 (HSPB6) Interaktionspartner * HSPB6 cDNA encodes a 168 aa protein that contains an alpha- ...
Top referenced Heat Shock Protein, alpha-Crystallin-Related, B6 ELISA Kits. Show all Heat Shock Protein, alpha-Crystallin- ... alpha-Crystallin-Related, B6 Antibodies (122) and Heat Shock Protein, alpha-Crystallin-Related, B6 Proteins (14) and many more ... alpha-Crystallin-Related, B6 Interaction Partners. Human Heat Shock Protein, alpha-Crystallin-Related, B6 (HSPB6) interaction ... Heat Shock Protein, alpha-Crystallin-Related, B6 (HSPB6) Antigen Profile Antigen Summary ...
N-terminal amino acid sequencing and immunoreactivity identified this protein as the 16-kDa alpha-crystallin-like small heat ... Collectively, these results suggest that the mycobacterial alpha-crystallin protein may play a role in enhancing long-term ... Stationary phase-associated protein expression in Mycobacterium tuberculosis: function of the mycobacterial alpha-crystallin ... Stationary phase-associated protein expression in Mycobacterium tuberculosis: function of the mycobacterial alpha-crystallin ...
... alpha A (CRYAA) Protein (His tag). Species: Sheep (Ovine). Source: Yeast. Order product ABIN1630960. ... Crystallin, alpha A (CRYAA) Background Recommended name: Alpha-crystallin A chain Cleaved into the following chain: 1.. Alpha- ... Crystallin, alpha A (CRYAA) (AA 1-173) protein (His tag) Crystallin, alpha A (CRYAA) (AA 1-173) protein (His tag). Details for ... More product categories related to Crystallin, alpha A Protein * 152 anti-Crystallin, alpha A Primary Antibodies ...
Most gamma crystallins bind to alpha crystallins under conditions favoring their unfolding, while some gamma crystallin ... Interaction of human gammaS-crystallin and its cataract-associated G18V mutant with alpha-crystallins ... Interaction of human gammaS-crystallin and its cataract-associated G18V mutant with alpha-crystallins ... The interaction of human gammaS-crystallin with alphaA-crystallin appears to differ from that of alphaB-crystallin. We ...
... peptide fragments derived from native α-crystallin were synthesized and explored as a peptide-based crystallin aggregation ... The aggregation of the α-crystallin protein is the pathological hallmark of cataract. In the current work, ... Carrier-free self-built aspirin nanorods as anti-aggregation agents towards alpha-crystallin-derived peptide aggregates: ... Carrier-free self-built aspirin nanorods as anti-aggregation agents towards alpha-crystallin-derived peptide aggregates: ...
Alpha B Crystallin. By Technical Data. Alpha B Crystallin - Immunoassays. Alpha B Crystallin - Sandwich ELISA, Biotin-labelled ... Alpha B Crystallin - for Serum - Immunoassays. Alpha B Crystallin - for Tissue extract - Immunoassays. Alpha B Crystallin - for ... further divided into acidic and basic groups (Alpha-A and Alpha-B respectively). In the. lens, alpha-crystallin primarily ... The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat ...
"Alpha (α)-Crystallin Index" can be calculated, which is a measure of available α-crystallin in a living lens, and which is ... J. S. Zigler, M. B. Datiles, III, R. R. Ansari, K. I. Suh, S. Vitale, G. F. Reed, F. L. Ferris, III; The Alpha-Crystallin Index ... Conclusions:: The αCI, a measure of the amount of clinically measurable alpha-crystallin in the lens, statistically ... The Alpha-Crystallin Index (CI): A New Parameter to Assess Susceptibility to Cataract ...
HSPB6; heat shock protein, alpha-crystallin-related, B6; Hsp20; FLJ32389; heat shock protein beta-6; heat shock 20 kDa-like ...
Regulation of the mouse alpha A-crystallin gene: isolation of a cDNA encoding a protein that binds to a cis sequence motif ... Our results suggest that the lens-specific alpha A-crystallin, MHC class I, beta interferon and other genes have a similar cis- ... Regulation of the mouse alpha A-crystallin gene: isolation of a cDNA encoding a protein that binds to a cis sequence motif ... Regulation of the mouse alpha A-crystallin gene: isolation of a cDNA encoding a protein that binds to a cis sequence motif ...
Shop QEDs high-quality Alpha B Crystallin antibodies, for use in WB & ELISA applications. With 20+ years of expertise, you can ... Alpha crystallins are further divided into acidic (Alpha A) and basic (Alpha B) groups. In the lens, alpha crystallin maintains ... Both alpha A and alpha B crystallin prevent apoptosis by inhibiting caspases. Alpha B crystallin is found in many cells and ... It does not cross-react with alpha A crystallin, beta- L crystallin, beta-H crystallin, gamma crystallin, Hsp25, Hsp27, or ...
Recombinant Protein and Alpha-crystallin Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are ... Alpha-crystallin. Alpha-crystallin ELISA Kit. Alpha-crystallin Recombinant. Alpha-crystallin Antibody. Also known as Alpha- ... Alpha-crystallin A chain. Alpha-crystallin A chain ELISA Kit. Alpha-crystallin A chain Recombinant. Alpha-crystallin A chain ... Alpha-crystallin B chain. Alpha-crystallin B chain ELISA Kit. Alpha-crystallin B chain Recombinant. Alpha-crystallin B chain ...
We identified a previously reported oligomerization disrupting mutation, c.62G > A (p.R21Q), in the Crystallin alpha A (CRYAA) ... The work strengthens the genotype-phenotype correlations between this functional mutation in the crystallin alpha A (CRYAA) ... Andley UP, Song Z, Wawrousek EF, Fleming TP, Bassnett S. Differential protective activity of alpha A- and alpha B-crystallin in ... alignments of crystallin alpha A protein sequence of the region of interest from seven species to the human crystallin alpha A ...
The association of alpha B-crystallin (CRYAB), a gene identified as being differentially expressed by gene profiling of NPC and ... The association of alpha B-crystallin (CRYAB), a gene identified as being differentially expressed by gene profiling of NPC and ... alpha-Crystallin B Chain - physiology. -. dc.title. Identification of tumor suppressive activity by irradiation microcell- ... Alpha B-crystallin (CRYAB). Chromosome 11q. Irradiation microcell-mediated chromosome transfer. Microcell hybrid. ...
Human alpha-crystallins were separated from fetal, young, senile nondiabetic and diabetic lenses. The effects of aging and ... Fluorescence study of the effects of aging and diabetes mellitus on human lens alpha-crystallin.. *. J Liang ... Human alpha-crystallins were separated from fetal, young, senile nondiabetic and diabetic lenses. The effects of aging and ... Fluorescence study of the effects of aging and diabetes mellitus on human lens alpha-crystallin.}, author={J Liang}, journal={ ...
ALPHA-B CRYSTALLIN-RELATED description, symptoms and related genes. Get the complete information i ... Alpha-b Crystallin-related Is also known as mfm, fatal infantile hypertonic, alpha-b crystallin-related;. ... Myopathy, myofibrillar, fatal infantile hypertrophy, alpha-B crystallin-related (sequence analysis of CRYAB gene). By CGC ... Myofibrillar myopathy, alpha-B-crystallin related. By Clinic of Pediatrics and Adolescent Medicine General University Hospital ...
Recombinant alpha A- and alpha B-crystallins in molar ratios of alpha A to alpha B at 1:1, corresponding to the aged sample, ... Recombinant alpha A- and alpha B-crystallins in molar ratios of alpha A to alpha B at 1:1, corresponding to the aged sample, ... Recombinant alpha A- and alpha B-crystallins in molar ratios of alpha A to alpha B at 1:1, corresponding to the aged sample, ... Recombinant alpha A- and alpha B-crystallins in molar ratios of alpha A to alpha B at 1:1, corresponding to the aged sample, ...
Crystallin alpha B), Human, Recombinant, E.coli Source Recombinant \ CRA30025 for more molecular products just contact us ... Crystallin alpha B), Human, Recombinant, E.coli Source Recombinant. Related products : CRYAB(Crystallin alpha B), Human, ... Crystallin alpha B), Human, Recombinant, E.coli Source Recombinant / Product Detail : CRA30025 CRYAB(Crystallin alpha B), Human ... CRYAB(Crystallin alpha B), Human, Recombinant, E.coli Source Recombinant recombinant proteins Most stable storage is at - 81 C ...
Alpha B-crystallin expression in human and rat hepatic stellate cells. J Hepatol. 2001;35(2):200-7.PubMedCrossRefGoogle Scholar ... ABCRYS (alpha B-crystallin). In normal and cirrhotic human livers, perisinusoidally located, stellate-shaped cells were stained ... Alpha-smooth-muscle actin expression in normal and fibrotic human livers. Dig Dis Sci. 1993;38(8):1473-9.PubMedCrossRefGoogle ... Appearance of alpha-smooth-muscle-actin-positive cells in hepatic fibrosis. Liver. 1991;11(2):100-5.PubMedCrossRefGoogle ...
... Dev Neurobiol. 2015 May; ... For the best crystallin candidates, we tested a potential causal role in CF lens apoptosis. Crybgx, crybb1c (not expressed in ... We reasoned that crystallins, the major lens structural proteins, could be defective or misregulated. We surveyed the CF and SF ... Altogether, these data suggest that crystallin sequence evolution and expression defects may contribute to the loss of eyes in ...
  • Defects in CRYAB are the cause of myofibrillar alpha-B crystallin-related (MFM-CRYAB) [MIM:608810]. (abcam.com)
  • The hamster alpha-B crystallin (CRYAB) gene, which is highly homologous to that of humans, was cloned at first by Quax-Jeuken and it was demonstrated that the coding region of CRYAB consists of three exons (Quax-Jeuken et al. (atlasgeneticsoncology.org)
  • This provided the assumption that CRYAB is composed of a highly conserved "alpha crystallin core" domain consisting of about 80 to 100 residues with a beta-sandwich structure (Ghosh et al. (atlasgeneticsoncology.org)
  • CRYAB as well as alpha-A crystallin contribute to ~35% of all vertebrate lens proteins. (atlasgeneticsoncology.org)
  • CRYAB exhibits a high homology of 56% to alpha-A-crystallin. (atlasgeneticsoncology.org)
  • CRYAB(Crystallin alpha B), Human, Recombinant, E.coli Source Recombinant recombinant proteins Most stable storage is at - 81 C or lower but some lyophilised proteins can be stored at +4C. (antibody-antibodies.com)
  • Please read carefully the data sheet of the CRYAB(Crystallin alpha B), Human, Recombinant, E.coli Source Recombinant. (antibody-antibodies.com)
  • CRYAB(Crystallin alpha B), Human, Recombinant, E.coli Source Recombinant Human samples 80 % of the research is conducted on human samples. (antibody-antibodies.com)
  • GENTAUR suppliers human normal cells, cell lines, RNA extracts and lots of antibodies and ELISA kits to Human proteins as well as CRYAB(Crystallin alpha B), Human, Recombinant, E.coli Source Recombinant. (antibody-antibodies.com)
  • Alpha B-Crystallin (CRYAB) is purported to be a metastasis suppressor protein, and lack or lower CRYAB expression is a prognostic biomarker for several types of cancer, such as that of the prostate and head and neck. (cdc.gov)
  • Analysis of the composition of drusen deposits and surrounding retinal pigment epithelia (RPE) layer cells in humans with AMD identified amyloid beta (Aβ) ( 2 ), alpha b crystallin (CRYAB), and alpha a crystalline (CRYAA) ( 3 , 4 ) as the most significant components of this pathology. (frontiersin.org)
  • In the 855Met data set, αB-crystallin gene ( CRYAB) expression was an independent predictor of brain as the first distant site of relapse (hazards ratio, HR=1.2, (95% confidence interval, CI 1.0-1.4), P =0.021). (nature.com)
  • Recombinant full length protein (His-T7-tag) corresponding to Human Alpha B Crystallin aa 1-175. (abcam.com)
  • Recombinant Human Alpha B Crystallin protein ( ab48779 ) can be used as a positive control in WB. (abcam.com)
  • Crystallins are a highly conserved protein family consisting of three classes, alpha, beta and gamma. (atlasgeneticsoncology.org)
  • A gene on chromosome 11q22.3-q23.1 that encodes alpha crystallin B, a protein induced by heat shock that belongs to the small heat shock protein (sHSP, or HSP20) family. (thefreedictionary.com)
  • Alpha B crystallin is a lens protein which has some homology with the small heat shock proteins. (ihcworld.com)
  • The role of Alpha B crystallin in these inclusion bodies is unknown, however, it may function as an accessory protein for intermediate filament aggregation. (ihcworld.com)
  • Zusätzlich bieten wir Ihnen Heat Shock Protein, alpha-Crystallin-Related, B6 Antikörper (120) und Heat Shock Protein, alpha-Crystallin-Related, B6 Proteine (14) und viele weitere Produktgruppen zu diesem Protein an. (antikoerper-online.de)
  • peptides in heat-shock protein Hsp20 (G71HFSVLLDVKHFSPEEIAVK91) and Hsp27 (D93RWRVSLDVNHFAPDELTVK113) with sequence homology to alpha-crystallin also have robust chaperone and anti-apoptotic activities. (antikoerper-online.de)
  • These findings strongly suggest that HSP20 might decrease the IKK-alpha protein level and that it down-regulates the TNF-alpha-stimulated intracellular signaling in HCC, thus resulting in the suppression of HCC progression. (antikoerper-online.de)
  • Additionally we are shipping Heat Shock Protein, alpha-Crystallin-Related, B6 Antibodies (122) and Heat Shock Protein, alpha-Crystallin-Related, B6 Proteins (14) and many more products for this protein. (antibodies-online.com)
  • Stationary phase-associated protein expression in Mycobacterium tuberculosis: function of the mycobacterial alpha-crystallin homolog. (asm.org)
  • N-terminal amino acid sequencing and immunoreactivity identified this protein as the 16-kDa alpha-crystallin-like small heat shock protein. (asm.org)
  • Collectively, these results suggest that the mycobacterial alpha-crystallin protein may play a role in enhancing long-term protein stability and therefore long-term survival of M. tuberculosis. (asm.org)
  • The aggregation of the α-crystallin protein is the pathological hallmark of cataract. (rsc.org)
  • The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. (biovendor.com)
  • When cellular stress occurs, alpha-crystallin enters its' phosphorylated state and may serve a structural control function and play a role in protein maintenance (2). (biovendor.com)
  • In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA (3-7). (biovendor.com)
  • The first peak consists of smaller particles, primarily composed of the α-crystallin lens protein fraction. (arvojournals.org)
  • α-crystallins have been shown to be the internal molecular chaperone proteins which prevent lens protein aggregation and cataract formation. (arvojournals.org)
  • Regulation of the mouse alpha A-crystallin gene: isolation of a cDNA encoding a protein that binds to a cis sequence motif shared with the major histocompatibility complex class I gene and other genes. (asm.org)
  • Gel retardation experiments with synthetic oligodeoxynucleotides revealed a mouse lens nuclear protein which bound specifically to the palindromic sequence 5'-GGGAAATCCC-3' at positions -66 to -57 in the alpha A-crystallin promoter. (asm.org)
  • It encoded a protein, tentatively called alpha A-CRYBP1, containing at least two zinc fingers. (asm.org)
  • In response to cellular stress, alpha crystallin is phosphorlyated and may serve a structural control function and play a role in protein maintenance. (qedbio.com)
  • Mouse anti Human Alpha A Crystallin antibody, clone c9F2 recognizes alpha A crystallin, a 20 kDa water-soluble major structural protein and member of the heat shock protein family, designated HSPB4, which acts as a molecular chaperone and is believed to play a role in the maintenance of ocular lens transparency/refractive index. (bio-rad-antibodies.com)
  • Besides the involvement of both alpha A and alpha B crystallin in the prevention of protein precipitation and cataract formation in the eye, alpha crystallins have also been demonstrated in other tissues including the heart, brain, kidney, spleen and thymus. (bio-rad-antibodies.com)
  • E. coli -derived recombinant protein corresponding to full-length (aa1-173) human crystallin alpha A. (bio-rad-antibodies.com)
  • 1998). Genealogy of the alpha-crystallin - small heat-shock protein superfamily. (core.ac.uk)
  • Background Mutations in αB-crystallin result in proteotoxic cardiomyopathy with desmin mislocalization to protein aggregates. (nih.gov)
  • Methods and Results Mice overexpressing the R120G mutant of αB-crystallin in cardiomyocytes ( Myh6-Cry ABR 120G) were subjected to IF or ad-lib feeding, or transduced with adeno-associated virus- TFEB or adeno-associated virus-green fluorescent protein after development of advanced proteotoxic cardiomyopathy. (nih.gov)
  • Cry ABR 120G-expressing hearts demonstrated increased interaction of desmin with αB-crystallin and reduced interaction with chaperone protein, HSPB 8, compared with wild type, which was reversed by both IF and TFEB transduction. (nih.gov)
  • Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. (nih.gov)
  • The chaperone-like protein alpha-crystallin is a approximately 35 subunit hetero-oligomer consisting of alphaA and alphaB subunits in a 3:1 molar ratio and has the function of maintaining eye lens transparency. (leidenuniv.nl)
  • However, the thermodynamic parameters obtained by DSC are inconsistent with the complete denaturation of an oligomeric protein of the size of alpha-crystallin. (leidenuniv.nl)
  • Mouse anti Human alpha B crystallin antibody recognizes alpha B crystallin, also known as heat shock protein beta-5, heat-shock 20 kD like-protein and renal carcinoma antigen NY-REN-27, rosenthal fiber component. (bio-rad-antibodies.com)
  • Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) family. (bio-rad-antibodies.com)
  • For 15 y, α B-crystallin (heat shock protein [Hsp] B5) has been labeled an autoantigen in multiple sclerosis (MS) based on humoral and cellular responses found in humans and animal models. (jimmunol.org)
  • The association of α B-crystallin (heat shock protein [Hsp] B5) with multiple sclerosis (MS) has been a puzzling story ( 1 ). (jimmunol.org)
  • Van Noort and colleagues ( 2 , 3 ) first proposed the protein to be an autoantigen in 1995 based on the reactivity of PBMCs from MS patients and healthy control subjects to proliferate in response to a fraction of myelin from MS brains containing α B-crystallin. (jimmunol.org)
  • Small heat-shock proteins, such as α B-crystallin, act as chaperones to prevent protein aggregation and play a key role in the prevention of such protein disorganisation diseases. (hindawi.com)
  • In this study, we have explored the potential for chaperone activity of α B-crystallin to suppress the formation of protein aggregates. (hindawi.com)
  • We tested the ability of α B-crystallin to suppress the aggregation of a polyQ protein and α -synuclein in Drosophila . (hindawi.com)
  • Alpha-crystallins ( α -crystallins) are major protein components of the vertebrate eye lens. (hindawi.com)
  • The α B-crystallin protein is found to be overexpressed in many neurological diseases, and mutations in α A- or α B-crystallin can cause cataracts and myopathy [ 11 ]. (hindawi.com)
  • The α B-crystallin protein is a member of the small heat shock protein family, and it has a chaperone-like function [ 12 - 15 ]. (hindawi.com)
  • AlphaB-crystallin, a major lens protein, is found in the central nervous system (CNS) and is a major protein component of Rosenthal fibers (RF), intracytoplasmic inclusions within astrocytes. (nii.ac.jp)
  • Sequencing of the promoter and coding regions of the alphaB-crystallin genes in two patients revealed them to be of normal sequence, suggesting the accumulation of alphaB-crystallin in those brains is not due to any abnormality of the primary structure of the protein. (nii.ac.jp)
  • Publications] Tomokane,N.: 'Rosenthal fibers share epitopes with αB-crystallin,glial fibrillary acidic protein,and ubiquitin,but not with vimentin.Immunoelectron microscopy with colloidal gold. (nii.ac.jp)
  • [10] [11] There are three major domains to the desmin protein: a conserved alpha helix rod, a variable non alpha helix head, and a carboxy-terminal tail. (wikipedia.org)
  • This entry represents the alpha crystallin domain (ACD) found in heat shock protein beta-3 (HspB3) from vertebrates, also known as heat-shock protein 27-like protein (HSPL27, 17 kDa) [ PMID: 8972725 , PMID: 9858786 ]. (ebi.ac.uk)
  • The well-characterized small heat-shock protein, αB-crystallin, acts as a molecular chaperone by interacting with unfolding proteins to prevent their aggregation and precipitation. (edu.au)
  • The enhanced molecular chaperone activity of immobilized αB-crystallin has potential applications in preventing protein misfolding, including against amyloid disease processes, such as dialysis-related amyloidosis, and for biodiagnostic detection of misfolded proteins. (edu.au)
  • [email protected]#The protein expression of alpha B-crystallin was increased significantly, suggesting that it might play an important role in the progress of silicosis . (bvsalud.org)
  • Gamma-crystallin D is a protein that in humans is encoded by the CRYGD gene. (wikipedia.org)
  • Beta-crystallin A3 is a protein that in humans is encoded by the CRYBA1 gene. (wikipedia.org)
  • This gene, a beta acidic group member, encodes two proteins (crystallin, beta A3 and crystallin, beta A1) from a single mRNA, the latter protein is 17 aa shorter than crystallin, beta A3 and is generated by use of an alternate translation initiation site. (wikipedia.org)
  • Protection by αΒ-crystallin overexpression is linked to maintenance of proper mitochondrial protein levels, inhibition of abnormal mitochondrial permeability transition pore activation and maintenance of mitochondrial membrane potential (Δψ m ). (biologists.org)
  • These αB-crys Z104 mice express a transgene containing human alpha B crystallin under the direction of the mouse prion protein promoter and may be useful in studies of amyotrophic lateral sclerosis (ALS or Lou Gehrig's disease), protein aggregation and neuronal proteostasis. (jax.org)
  • The small heat-shock protein family member alpha B crystallin binds misfolded proteins and holds them in large soluble aggregates. (jax.org)
  • These transgenic mice express the human alpha B crystallin under the direction of the mouse prion protein promoter, producing approximately 6-fold overexpression of alpha beta crystallin (mouse and human) in the spinal cord, approximately 30-fold overexpression in the forebrain, and high levels of expression also detected in the cerebellum by Western blot analysis. (jax.org)
  • A transgenic construct containing cDNA of human alpha B crystallin under the control of the mouse Prnp , prion protein, gene promoter, was injected into fertilized C3/B6 F2 mouse eggs. (jax.org)
  • Substantially elevating the levels of alphaB-crystallin in spinal motor neurons of mutant SOD1 mice does not significantly delay paralysis or attenuate mutant protein aggregation. (jax.org)
  • The transgenic construct contains cDNA of human alpha B crystallin under the control of the mouse Prnp, prion protein, gene promoter. (jax.org)
  • This protein binds to other, damaged proteins in the eye, preventing them from congregating and becoming cataracts, but α-crystallin deteriorates over time or because of adverse conditions. (photonics.com)
  • For this purpose, αB-crystallin which is eye lens protein is taken as a sample. (nii.ac.jp)
  • A method to prepare 75% deuterated αB-crystallin was established and then it was confirmed that this protein is scatteringly invisible in 100% D2O solution. (nii.ac.jp)
  • Generation of a biological association network evidenced several genes, such as connective tissue growth factor (Ctgf), tissue inhibitor of metalloproteinase 1 (Timp1), galanin (Gal), synaptotagmin 1 (Syt1), growth factor receptor bound protein 2 (Grb2), actin gamma 2 (Actg2) and smooth muscle alpha actin (Acta2), as highly interconnected nodes of the resulting network. (biomedcentral.com)
  • A method for expressing proteins as a fusion chimera with a domain of p26 or alpha crystallin type proteins to improve the protein stability and solubility when over expressed in bacteria such as E. coli is provided. (google.com)
  • Genes of interest are cloned into the multiple cloning site of the Vector System just downstream of the p26 or alpha crystallin type protein and a thrombin cleavage site. (google.com)
  • 2. The device of claim 1 , wherein the alpha-A-crystallin protein is a bovine alpha-A-crystallin protein. (google.com)
  • 3. The device of claim 1 , wherein the alpha-A-crystallin protein has an ability to inhibit protease. (google.com)
  • 8. The device of claim 1 , wherein the resin material coupled to the alpha-A-crystallin protein is cyanogen bromide activated sepharose. (google.com)
  • 12. A method of preparing device according to claim 1 , comprising the steps of a) mixing an alpha-A-crystallin protein with a buffer to form a coupling buffer mixture, and b) contacting an agarose resin with the coupling buffer mixture to form a prepared filter resin. (google.com)
  • 16. The method of claim 12 , wherein the alpha-A-crystalline protein is a bovine alpha-A-crystallin protein. (google.com)
  • 17. The method of claim 12 , wherein the alpha-A-crystalline protein has an ability to inhibit protease. (google.com)
  • Alpha-B-crystallin is a major structural protein in the eye's lens. (stanford.edu)
  • They found that, in mice bioengineered to lack alpha-B-crystallin, experimentally induced stroke lesions were more massive than those induced in otherwise genetically similar mice whose cells were capable of making the protein. (stanford.edu)
  • It has been reported that about half of mutations in crystallins and a quarter in connexins (gap junction proteins), with the remainder divided among the genes for heat shock transcription factor-4 (HSF4), aquaporin-0 (AQP0, MIP), and beaded filament structural protein-2 (BFSP2) [2] , [3] . (plos.org)
  • The α-crystallins are large protein complexes in the lens composed of αA- and αB-crystallins. (plos.org)
  • In addition to their structural roles, α-crystallins are members of the small heat shock protein family and exhibit important molecular chaperone activity within the lens [4] . (plos.org)
  • CRYGA (Crystallin Gamma A) is a Protein Coding gene. (genecards.org)
  • CRYBA1 (Crystallin Beta A1) is a Protein Coding gene. (genecards.org)
  • αB-crystallin protein expression was determined by immunohistochemistry using the clinically annotated British Columbia Cancer Agency (BCCA) tissue microarray ( n =3,987 breast tumors). (nature.com)
  • In the BCCA series, αB-crystallin protein expression was an independent prognostic marker of poor breast cancer-specific survival (HR=1.3, (95% CI 1.1-1.6), P =0.014). (nature.com)
  • αB-crystallin is a widely expressed member of the small heat shock protein family that protects cells from stress by its dual function as a molecular chaperone to preserve proteostasis and as a cell death antagonist that inhibits caspase-3 activation and oxidative stress. (nature.com)
  • Studies on the alpha-crystallin target protein binding sites: sequential binding with two target proteins. (ccmb.res.in)
  • [8] A conserved protein binding domain of approximately 80 amino-acid alpha crystallins are known as small heat shock proteins (sHSP). (wikipedia.org)
  • Chemically, these fibers are composed of glial fibrillary acidic protein and alpha-beta crystallin. (medscape.com)
  • αA- and αB-crystallins, the major lens structure proteins and members of the small heat-shock proteins (sHSPs) family, play essential roles in maintaining normal cellular structure and physiology of both ocular and some non-ocular tissues. (eurekaselect.com)
  • Alpha crystallins have features of molecular chaperones, but do not renature and release proteins like true chaperones. (thefreedictionary.com)
  • We therefore conclude that the molecular chaperones, alphaA and alphaB-crystallins exhibit different specificities towards the same set of substrate or target proteins. (arvojournals.org)
  • In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. (biovendor.com)
  • alpha A-CRYBP1 is either homologous or very similar to the human nuclear proteins MBP-1 (Baldwin et al. (asm.org)
  • Our results suggest that the lens-specific alpha A-crystallin, MHC class I, beta interferon and other genes have a similar cis-acting DNA regulatory motif that shares alpha A-CRYBPI, MBP-1, PRDII-BF1, HIV-EP1, or other closely related proteins as trans-acting factors. (asm.org)
  • We reasoned that crystallins, the major lens structural proteins, could be defective or misregulated. (nih.gov)
  • Western blotting analysis of recombinant crystallin proteins and mouse eye derived lysates probed with Mouse anti Human alpha crystallin ( MCA2696 ). (bio-rad-antibodies.com)
  • 2002). Functional similarities between the small heat shock proteins Mycobacterium tuberculosis HSP 16.3 and human alphaB-crystallin. (core.ac.uk)
  • 2003). human genome encodes 10 alpha-crystallin-related small heat shock proteins: HspB1-10. (core.ac.uk)
  • Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. (nih.gov)
  • Gamma-crystallins are a homogeneous group of highly symmetrical, monomeric proteins typically lacking connecting peptides and terminal extensions. (nih.gov)
  • Therefore, α B-crystallin can bind to the unfolded proteins and inhibit nonspecific aggregation [ 10 ]. (hindawi.com)
  • These C-terminal truncations decrease chaperone activity of alpha-crystallins, possibly leading to the observed increases in insoluble proteins during aging and cataract. (elsevier.com)
  • Alpha-crystallin-type heat shock proteins: socializing minichaperones in the context of a multichaperone network. (ebi.ac.uk)
  • Immobilized αB-crystallin exhibited a significant enhancement (up to 5000-fold, when compared with the equivalent activity of αB-crystallin in solution) of its chaperone activity against various proteins undergoing both amorphous and amyloid fibril forms of aggregation. (edu.au)
  • Twenty of them were analyzed by MALDI -TOF-MS. A total of 13 altered proteins were identified, including alpha B-crystallin , mast cell protease 6, annexin 1, etc. (bvsalud.org)
  • Although crystallins are highly conserved proteins among vertebrates, a significant number of polymorphisms exist in human population. (bjbms.org)
  • αB-Crystallin (αB) and HSP27 belong to the class of small Heat Shock Proteins (sHSPs) whose levels of expression increase under conditions of cellular stress, viz. (washington.edu)
  • Although the full extent of the functions of αB-Crystallin (αB) and HSP27 remain to be determined, the proteins are each implicated in certain inherited forms of dystrophies and αB is also implicated in breast and other forms of cancer. (washington.edu)
  • The lens crystallins are proteins that make the lens of the eye clear and refractive. (whozoo.org)
  • The solubility and stability of crystallin proteins play critical roles in maintaining the optical transparency of the lens during the life span. (plos.org)
  • Crystallin proteins, including α-, β- and γ-crystallins, represent about 90% of lens soluble proteins in human. (plos.org)
  • β-crystallins are the most abundant water-soluble proteins in the lens and most expressed in lens cortical fiber cells. (plos.org)
  • Induction and phosphorylation of the small heat shock proteins HspB1/Hsp25 and HspB5/αB-crystallin in the rat retina upon optic nerve injury. (uni-ulm.de)
  • Schmidt T, Bartelt-Kirbach B, Golenhofen N (2012) Phosphorylation dependent subcellular localization of the small heat shock proteins HspB1/Hsp25 and HspB5/aB-crystallin in cultured hippocampal neurons. (uni-ulm.de)
  • This contrasts with its interaction with alphaB-crystallin which it has been shown to bind. (arvojournals.org)
  • The interaction of human gammaS-crystallin with alphaA-crystallin appears to differ from that of alphaB-crystallin. (arvojournals.org)
  • 2004). Association of the chaperone alphaB-crystallin with titin in heart muscle. (core.ac.uk)
  • 2004). Desmin aggregate formation by R120G alphaB-crystallin is caused by altered filament interactions and is dependent upon network status in cells. (core.ac.uk)
  • Molecular analysis of alphaB-crystallin in central nervous system and in pathologic conditions. (nii.ac.jp)
  • A number of human brains displaying a variety of pathologic changes were examine by immunohistochemistry with an anti-alphaB-crystallin antiserum and increased immunoreactivity was found in astrocytes and oligodendrocytes without the formation of RFs. (nii.ac.jp)
  • Furthermore, some neurons in neurodegenerative disorders were also immunolabeled with anti-alphaB-crystallin antiserum. (nii.ac.jp)
  • We also determined the structures of alphaB-crystallin mRNAs and the genomic structure. (nii.ac.jp)
  • Two major classes of mRNAs for the alphaB-crystallin, about 0.9 and 1.2 kilobases in length, are expressed in rat brain and they differ in the lengths of their 5' leader sequences. (nii.ac.jp)
  • Since there is only a single copy of the alphaB-crystallin gene, the two classes of mRNAs are generated by alternative transcriptional initiation from different promoters and their expressions are regulated differentially. (nii.ac.jp)
  • Next, we examined primary structures of alphaB-crystallin in two cases of pathologically confirmed Alexander's disease. (nii.ac.jp)
  • Interactions of HSP22 (HSPB8) with HSP20, alphaB-crystallin, and HSPB3. (ebi.ac.uk)
  • Hsp27 (HspB1) and alphaB-crystallin (HspB5) as therapeutic targets. (ebi.ac.uk)
  • Bartelt-Kirbach B, Slowik A, Beyer C, Golenhofen N (2017), Upregulation and phosphorylation of HspB1/Hsp25 and HspB5/alphaB-crystallin after transient middle cerebral artery occlusion in rats. (uni-ulm.de)
  • HspB5/alphaB-crystallin increases dendritic complexity and protects the dendritic arbor during heat shock in cultured rat hippocampal neurons. (uni-ulm.de)
  • This antibody recognizes human and bovine alpha B crystallin. (qedbio.com)
  • Immunocytochemistry/ Immunofluorescence: AlphaA Crystallin/CRYAA Antibody (1H3.B8) [NBP2-12875] - Tissue: Neuroblastoma cell line SK-N-BE. (novusbio.com)
  • 0.5 ug/ml was sufficient for detection of 100 ng purified alphaA crystalline by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.Alpha A Crystallin Antibody.Use in Immunoblotting reported in scientific literature (PMID 28546921). (novusbio.com)
  • Western blot analysis of whole cell lysates probed with alpha B crystallin antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P ) and visualized on the ChemiDoc MP with 4 second exposure. (bio-rad-antibodies.com)
  • Mouse anti Human alpha B crystallin chain antibody detects a band of 22 kDa. (bio-rad-antibodies.com)
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Beta-crystallin A4(TA308870) antibody at 1:200 dilution. (acris-antibodies.com)
  • We have shown by site-directed mutagenesis that the sequence between positions -69 and -40 of the mouse alpha A-crystallin gene is crucial for tissue-specific gene expression in a transfected mouse lens epithelial cell line transformed with the early region of simian virus 40. (asm.org)
  • A (p.R21Q), in the Crystallin alpha A ( CRYAA ) gene segregating in this three generation family. (biomedcentral.com)
  • The work strengthens the genotype-phenotype correlations between this functional mutation in the crystallin alpha A ( CRYAA ) gene and paediatric cataract. (biomedcentral.com)
  • Four gamma-crystallin genes (gamma-A through gamma-D) and three pseudogenes (gamma-E, gamma-F, gamma-G) are tandemly organized in a genomic segment as a gene cluster. (nih.gov)
  • Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. (bio-rad-antibodies.com)
  • Mutation screening and genotype phenotype correlation of alpha-crystallin, gamma-crystallin and GJA8 gene in congenital cataract. (bjbms.org)
  • Entrez Gene: CRYGD crystallin, gamma D". CS1 maint: discouraged parameter (link) Graw J (1998). (wikipedia.org)
  • Mutation screening of the candidate genes detected a heterozygous c.465G→C change in the exon6 of the βB2-crystallin gene (CRYBB2) in all family members affected with cataracts, resulting in the substitution of a highly conserved Tryptophan to Cystine (p.W151C). (plos.org)
  • Taken together, these data suggest that the missense mutation in CRYBB2 gene leads to progressive congenital membranous cataract by impacting the solubility and function of βB2-crystallin. (plos.org)
  • The highest logarithm of odds score (1.5) region 2q34-36.1, spanning the crystallin beta A2 ( CRYBA2 ) gene, showed no sequence changes. (molvis.org)
  • Expression of the hspb4 gene, which codes for alpha crystallin , increases considerably in the lens in response to heat shock. (wikipedia.org)
  • Recombinant human gammaS- and alphaA- crystallins were expressed in E. coli. (arvojournals.org)
  • Recombinant alpha A- and alpha B-crystallins in molar ratios of alpha A to alpha B at 1:1, corresponding to the aged sample, were also studied in detail. (elsevier.com)
  • Even when recombinant alpha-crystallins were mixed in molar ratios equivalent to those found in vivo, similar S values as the native aged alpha-crystallin aggregates were not obtained. (elsevier.com)
  • Alpha-crystallin from young donors, and recombinant alpha A- and alpha B-crystallin and their mixtures showed chaperone activity, which was markedly lost in samples from the aged alpha-crystallin aggregates. (elsevier.com)
  • Expression of recombinant -crystallin in escherichia coli with the help of groel/es and its purification. (ccmb.res.in)
  • The alpha crystallins are divided into two groups according to their acidic as well as basic character, namely A and B. The genes encoding the alpha crystallins A and B are located at chromosome 11 and 21, respectively. (atlasgeneticsoncology.org)
  • Whether due to aging or mutations in specific genes, gamma-crystallins have been involved in cataract formation. (nih.gov)
  • Polymorphisms of the gamma crystallin A and B genes among Indian patients with pediatric cataract. (nih.gov)
  • Genes associated with the development of lens include crystallin genes. (bjbms.org)
  • 1991). "Human gamma-crystallin genes. (wikipedia.org)
  • Because of their high levels of expression, crystallins represent compelling candidate genes for inherited cataracts. (molvis.org)
  • Different mutations in crystallin genes could lead to distinctive cataract phenotypes. (molvis.org)
  • Recommended name: Alpha-crystallin A chain Cleaved into the following chain: 1. (antibodies-online.com)
  • Also known as Alpha-crystallin B chain (Alpha(B)-crystallin). (mybiosource.com)
  • Publications] Iwaki,A.: 'Multiple mRNAs of rat brain α-crystallin B-chain result from alternative transcriptional initiation. (nii.ac.jp)
  • The anti-aggregation potential of aspirin was evaluated towards these peptide-generated aggregates as well as towards the α-crystallin aggregate. (rsc.org)
  • Lens alpha-crystallin, composed of two subunits alpha A- and alpha B-crystallin, forms large aggregates in the lens of the eye. (elsevier.com)
  • Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. (wikipedia.org)
  • Wild type (wt) and W151C mutant βB2-crystallin were expressed in human lens epithelial cells (HLECs), and the fluorescence results showed that Wt-βB2-crystallin was evenly distributed throughout the cells, whereas approximately 34.7% of cells transfected with the W151C mutant βB2-crystallin formed intracellular aggregates. (plos.org)
  • The alpha-crystallins possess structural and functional similarities with Hsp25 and Hsp27 (1). (biovendor.com)
  • 2004). HSP27 but not HSP70 has a potent protective effect against alpha-synuclein-induced cell death in mammalian neuronal cells. (core.ac.uk)
  • This entry represents the alpha crystallin domain (ACD) found in mammalian Hsp27 (also denoted HspB1 in human). (ebi.ac.uk)
  • Thus alphaA- and alphaB-crystallins appear to behave differently with gammaS-crystallin and its G18V mutant. (arvojournals.org)
  • Fluorescence study of the effects of aging and diabetes mellitus on human lens alpha-crystallin. (semanticscholar.org)
  • The present study investigated the aggregate of human lens alpha-crystallin from elderly and young donors. (elsevier.com)
  • Novel crystallin gamma B mutations in a Kuwaiti family with autosomal dominant congenital cataracts reveal genetic and clinical heterogeneity. (nih.gov)
  • Tested in animal models and in patients with varying grades of cataracts, it was found to detect changes in the amount of α-crystallin, even when the lens of the eye is still clear. (photonics.com)
  • Previous studies have shown that approximately 8.3%∼25% of congenital cataracts are inherited, and mutations in crystallins are the most common. (plos.org)
  • HspB5 also precipitated Ig heavy and L chains from sera from patients with MS. These results establish that small Hsps bind Igs with high affinity and refute much of the serological data used to assign α B-crystallin as an autoantigen. (jimmunol.org)
  • Golenhofen N , Bartelt-Kirbach B. (2015) HspB5/alpha-B-crystallin in the brain. (uni-ulm.de)
  • Dieterich LC, Huang H, Massena S, Golenhofen N , Phillipson M, Dimberg A (2013) B-crystallin/HspB5 regulates endothelial-leukocyte interactions by enhancing NF-kB-induced up-regulation of adhesion molecules ICAM-1, VCAM-1 and E-selectin. (uni-ulm.de)
  • Changes in the self-association of alpha-crystallin aggregate were correlated to changes in chaperone activity. (elsevier.com)
  • Molecular chaperone activity of lens alpha-crystallins is reduced by loss of the C terminus. (elsevier.com)
  • Structural perturbation (e.g., partial unfolding) enhances the in vitro chaperone activity of αB-crystallin. (edu.au)
  • αB-crystallin was immobilized onto a range of solid material surfaces, and its characteristics and chaperone activity were assessed. (edu.au)
  • In the current work, peptide fragments derived from native α-crystallin were synthesized and explored as a peptide-based crystallin aggregation model towards cataract. (rsc.org)
  • The results demonstrated that aspirin had the capacity to inhibit crystallin and crystallin-derived peptide aggregation and could act as a potential therapeutic agent in mitigating cataract. (rsc.org)
  • The formation of self-built aspirin nanorods was also explored and their ability to inhibit the aggregation of model cataract peptides and α-crystallin aggregation was validated. (rsc.org)
  • Furthermore, by using histochemical staining we have determined that α B-crystallin inhibits the aggregation of polyQ in vivo . (hindawi.com)
  • Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases (8). (biovendor.com)
  • Both alpha A and alpha B crystallin prevent apoptosis by inhibiting caspases. (qedbio.com)
  • 3. Ishiguro Y, Kato K, Akatsuka H, Iwata H, Nagaya M. (1997) Chemotherapy-induced expression of alpha B-crystallin in neuroblastoma. (ihcworld.com)
  • 1991) Expression of alpha B-crystallin in the developing rat kidney. (ihcworld.com)
  • The expression of alpha B-crystallin in lung was further verified by Western blot . (bvsalud.org)
  • beta and gamma crystallins are also considered as a superfamily. (nih.gov)
  • The β- and γ-crystallins are recognized as members of a related β/γ-crystallin superfamily. (plos.org)
  • The purpose of this experiment was to 1) determine the cleavage sites produced in vitro by ubiquitous m-calpain and lens-specific Lp82 on alpha-crystallins, 2) identify alpha-crystallin cleavage sites produced in vivo during maturation and cataract formation in rat lens, and 3) estimate the relative activities of Lp82 and m-calpain by appearance of protease-specific cleavage products in vivo. (elsevier.com)
  • Using uniquely truncated alphaA-crystallins as in vivo markers, Lp82 and m-calpain were both found to be active during normal maturation of rat lens, whereas Lp82 seemed especially active during selenite cataract formation. (elsevier.com)
  • Together, these studies strongly suggest that during human cataract formation, covalent changes occur in the C-terminal region of the alpha A2 molecule. (scripps.edu)
  • Gamma-crystallins are involved in cataract formation. (prospecbio.com)
  • the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. (bio-rad-antibodies.com)
  • Packing-induced conformational and functional changes in the subunits of alpha-crystallin. (ccmb.res.in)
  • Species-specific structural and functional divergence of α-crystallins: Zebrafish αBa- and rodent αA(ins)-crystallin encode activated chaperones. (bjbms.org)
  • Furthermore, accumulation of α B-crystallin is an important mechanism for the prevention of cellular damage associated with heat shock and oxidative stress conditions in the trabecular meshwork [ 19 , 20 ]. (hindawi.com)
  • Most gamma crystallins bind to alpha crystallins under conditions favoring their unfolding, while some gamma crystallin variants in their native state also bind alpha crystallins. (arvojournals.org)
  • Preliminary studies indicate that gammaS-crystallin binds to alphaA-crystallin under thermal-unfolding conditions, like the other gamma-crystallins. (arvojournals.org)
  • Prediction of possible sites for posttranslational modifications in human gamma crystallins: effect of glycation on the structure of human gamma-B-crystallin as analyzed by molecular modeling. (nih.gov)
  • Alpha and beta families are further divided into acidic and basic groups (Alpha-A and Alpha-B respectively). (biovendor.com)
  • Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group). (wikipedia.org)
  • Also known as Alpha-crystallin (Acr) (14 kDa antigen) (16 kDa antigen) (HSP 16.3) (Nox16). (mybiosource.com)
  • They are characterised by the presence of an alpha crystallin domain (ACD) [ PMID: 11875128 ]. (ebi.ac.uk)
  • Conclusions IF and TFEB activation are clinically relevant therapeutic strategies to rescue advanced R120G αB-crystallin mutant-induced cardiomyopathy by normalizing desmin localization via autophagy-dependent and autophagy-independent mechanisms. (nih.gov)
  • Desmin has been shown to interact with desmoplakin [14] and αB-crystallin . (wikipedia.org)
  • To address this hypothesis, we investigated the consequences of αΒ-crystallin overexpression in the desmin-deficient ( Des −/− ) mouse model, which possesses a combination of the pathologies found in most cardiomyopathies, with mitochondrial defects as a hallmark. (biologists.org)
  • Furthermore, we found that both desmin and αΒ-crystallin are localized at sarcoplasmic reticulum (SR)-mitochondria-associated membranes (MAMs), where they interact with VDAC, Mic60 - the core component of mitochondrial contact site and cristae organizing system (MICOS) complex - and ATP synthase, suggesting that these associations could be crucial in mitoprotection at different levels. (biologists.org)
  • Mitochondrial abnormalities seem to be a common feature of diseases in which desmin and/or αB-crystallin deficiencies are implicated. (biologists.org)
  • Altogether, these data suggest that crystallin sequence evolution and expression defects may contribute to the loss of eyes in CF. (nih.gov)
  • Sequence differences in the a-crystallin domains between metazoans and nonmetazoans are critical to the different mechanism of dimerization, suggesting that some structural features seen for Tsp36 may be generalized to other metazoan sHsps. (core.ac.uk)
  • In vitro proteolysis of alphaA-crystallin by Lp82 and m-calpain produced unique cleavage sites by removing 5 and 11 residues, respectively, from the C terminus. (elsevier.com)
  • Immobilization was achieved via a plasma-deposited thin polymeric interlayer containing aldehyde surface groups and reductive amination, leading to the covalent binding of αB-crystallin lysine residues to the surface aldehyde groups via Schiff-base linkages. (edu.au)
  • In this regard, αA- and αB-crystallins seem to function differentially or even oppositely during tumorigenesis, and diverse molecular mechanisms have been proposed to explain their roles in cell apoptosis, cell proliferation and tumor metastasis. (eurekaselect.com)
  • For the best crystallin candidates, we tested a potential causal role in CF lens apoptosis. (nih.gov)
  • Alpha B crystallin is found in many cells and organs outside the lens and is over- expressed in cells subjected to stress conditions and in several neurological disorders. (qedbio.com)
  • The alpha-B-crystallin-deficient mice had worse neurological function after the stroke than did the normal mice. (stanford.edu)
  • 1995) Tau, ubiquitin, and alpha B-crystallin immunohistochemistry define the principal causes of degenerative frontotemporal dementia. (ihcworld.com)
  • Immobilized αB-crystallin was characterized by X-ray photoelectron spectroscopy, atomic force microscopy, and quartz crystal microgravimetry, which showed that ∼300 ng cm -2 (dry mass) of oligomeric αB-crystallin was bound to the surface. (edu.au)
  • Mammalian lens cystallins are divided into alpha, beta and gamma families. (biovendor.com)
  • Alpha B crystallin is also reported to be found in ubiquitinated intermediate filament inclusion bodies, such as Lewy bodies (neurofilaments), Rosenthal fibres (glial filaments) and Mallory bodies (cytokeratins). (ihcworld.com)
  • Thus, the accumulation of Alpha B-crystallin appears to be part of the repertoire of reactive processes of CNS glial cells and some neurons in pathologic conditions. (nii.ac.jp)
  • To my knowledge, nobody had looked at concentrations of alpha-B-crystallin after a stroke, either in people or in an experimental animal model before. (stanford.edu)
  • High concentrations of closely packed crystallins are required for lens transparency and the ability to focus light on the retina. (molvis.org)
  • We found that α B-crystallin suppresses both the compound eye degeneration induced by polyQ and the α -synuclein-induced rough eye phenotype. (hindawi.com)
  • Alpha B-crystallin suppresses pressure overload cardiac hypertrophy. (visualsonics.com)