Steroidal compounds related to PROGESTERONE, the major mammalian progestational hormone. Progesterone congeners include important progesterone precursors in the biosynthetic pathways, metabolites, derivatives, and synthetic steroids with progestational activities.
A heterogeneous family of water-soluble structural proteins found in cells of the vertebrate lens. The presence of these proteins accounts for the transparency of the lens. The family is composed of four major groups, alpha, beta, gamma, and delta, and several minor groups, which are classed on the basis of size, charge, immunological properties, and vertebrate source. Alpha, beta, and delta crystallins occur in avian and reptilian lenses, while alpha, beta, and gamma crystallins occur in all other lenses.
A subclass of crystallins that provides the majority of refractive power and translucency to the lens (LENS, CRYSTALLINE) in VERTEBRATES. Alpha-crystallins also act as molecular chaperones that bind to denatured proteins, keep them in solution and thereby maintain the translucency of the lens. The proteins exist as large oligomers that are formed from ALPHA-CRYSTALLIN A CHAIN and ALPHA-CRYSTALLIN B CHAIN subunits.
A transparent, biconvex structure of the EYE, enclosed in a capsule and situated behind the IRIS and in front of the vitreous humor (VITREOUS BODY). It is slightly overlapped at its margin by the ciliary processes. Adaptation by the CILIARY BODY is crucial for OCULAR ACCOMMODATION.
The basic subunit of beta-crystallins.
The acidic subunit of beta-crystallins.
A subclass of crystallins that found in the lens (LENS, CRYSTALLINE) of VERTEBRATES. Gamma-crystallins are similar in structure to BETA-CRYSTALLINS in that they both form into a Greek key-like structure. They are composed of monomeric subunits.
A class of crystallins that provides refractive power and translucency to the lens (LENS, CRYSTALLINE) in VERTEBRATES. Beta-crystallins are similar in structure to GAMMA-CRYSTALLINS in that they both contain Greek key motifs. Beta-crystallins exist as oligomers formed from acidic (BETA-CRYSTALLIN A CHAIN) and basic (BETA-CRYSTALLIN B CHAIN) subunits.
A protein phytotoxin from the seeds of Ricinus communis, the castor oil plant. It agglutinates cells, is proteolytic, and causes lethal inflammation and hemorrhage if taken internally.
One of the subunits of alpha-crystallins. Unlike ALPHA-CRYSTALLIN B CHAIN the expression of ALPHA-CRYSTALLIN A CHAIN is limited primarily to the lens (LENS, CRYSTALLINE).
One of the alpha crystallin subunits. In addition to being expressed in the lens (LENS, CRYSTALLINE), alpha-crystallin B chain has been found in a variety of tissues such as HEART; BRAIN; MUSCLE; and KIDNEY. Accumulation of the protein in the brain is associated with NEURODEGENERATIVE DISEASES such as CREUTZFELDT-JAKOB SYNDROME and ALEXANDER DISEASE.
A subclass of crystallins found in the lens (LENS, CRYSTALLINE) in BIRDS and REPTILES. They are inactive forms of the enzyme argininosuccinate lyase.
Partial or complete opacity on or in the lens or capsule of one or both eyes, impairing vision or causing blindness. The many kinds of cataract are classified by their morphology (size, shape, location) or etiology (cause and time of occurrence). (Dorland, 27th ed)
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A toxic lectin from the seeds of jequirity, Abrus precatorius L. Very active poison. Five different proteins have so far been isolated: Abrus agglutinin, the component responsible for: hemagglutinating activity, & abrins a-d, the toxic principals each consisting of two peptide chains are held together by disulfide bonds.
A genus of poisonous snakes of the subfamily Elapinae of the family ELAPIDAE. They comprise the kraits. Twelve species are recognized and all inhabit southeast Asia. They are considered extremely dangerous. (Moore: Poisonous Snakes of the World, 1980, p120)
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Plasma glycoprotein member of the serpin superfamily which inhibits TRYPSIN; NEUTROPHIL ELASTASE; and other PROTEOLYTIC ENZYMES.
Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.
Measurement of the index of refraction (the ratio of the velocity of light or other radiation in the first of two media to its velocity in the second as it passes from one into the other).
A family of cellular proteins that mediate the correct assembly or disassembly of polypeptides and their associated ligands. Although they take part in the assembly process, molecular chaperones are not components of the final structures.
A family of transcription factors that contain regions rich in basic residues, LEUCINE ZIPPER domains, and HELIX-LOOP-HELIX MOTIFS.
A class of morphologically heterogeneous cytoplasmic particles in animal and plant tissues characterized by their content of hydrolytic enzymes and the structure-linked latency of these enzymes. The intracellular functions of lysosomes depend on their lytic potential. The single unit membrane of the lysosome acts as a barrier between the enzymes enclosed in the lysosome and the external substrate. The activity of the enzymes contained in lysosomes is limited or nil unless the vesicle in which they are enclosed is ruptured. Such rupture is supposed to be under metabolic (hormonal) control. (From Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
An intermediate filament protein found predominantly in smooth, skeletal, and cardiac muscle cells. Localized at the Z line. MW 50,000 to 55,000 is species dependent.
The segregation and degradation of damaged or unwanted cytoplasmic constituents by autophagic vacuoles (cytolysosomes) composed of LYSOSOMES containing cellular components in the process of digestion; it plays an important role in BIOLOGICAL METAMORPHOSIS of amphibians, in the removal of bone by osteoclasts, and in the degradation of normal cell components in nutritional deficiency states.
A form of CARDIAC MUSCLE disease that is characterized by ventricular dilation, VENTRICULAR DYSFUNCTION, and HEART FAILURE. Risk factors include SMOKING; ALCOHOL DRINKING; HYPERTENSION; INFECTION; PREGNANCY; and mutations in the LMNA gene encoding LAMIN TYPE A, a NUCLEAR LAMINA protein.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
A mutation in which a codon is mutated to one directing the incorporation of a different amino acid. This substitution may result in an inactive or unstable product. (From A Dictionary of Genetics, King & Stansfield, 5th ed)
Organic chemistry methodology that mimics the modular nature of various biosynthetic processes. It uses highly reliable and selective reactions designed to "click" i.e., rapidly join small modular units together in high yield, without offensive byproducts. In combination with COMBINATORIAL CHEMISTRY TECHNIQUES, it is used for the synthesis of new compounds and combinatorial libraries.
A mutation caused by the substitution of one nucleotide for another. This results in the DNA molecule having a change in a single base pair.
New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
Biochemical identification of mutational changes in a nucleotide sequence.
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
Proteins which are synthesized in eukaryotic organisms and bacteria in response to hyperthermia and other environmental stresses. They increase thermal tolerance and perform functions essential to cell survival under these conditions.

Alterations in the mouse and human proteome caused by Huntington's disease. (1/314)

Huntington's disease is an autosomal dominantly inherited disease that usually starts in midlife and inevitably leads to death. In our effort to identify proteins involved in processes upstream or downstream of the disease-causing huntingtin, we studied the proteome of a well established mouse model by large gel two-dimensional electrophoresis. We could demonstrate for the first time at the protein level that alpha1-antitrypsin and alphaB-crystalline both decrease in expression over the course of disease. Importantly, the alpha1-antitrypsin decrease in the brain precedes that in liver and testes in mice. Reduced expression of the serine protease inhibitors alpha1-antitrypsin and contraspin was found in liver, heart, and testes close to terminal disease. Decreased expression of the chaperone alphaB-crystallin was found exclusively in the brain. In three brain regions obtained post-mortem from Huntington's disease patients, alpha1-antitrypsin expression was also altered. Reduced expression of the major urinary proteins not found in the brain was seen in the liver of affected mice, demonstrating that the disease exerts its influence outside the brain of transgenic mice at the protein level. Maintaining alpha1-antitrypsin and alphaB-crystallin availability during the course of Huntington's disease might prevent neuronal cell death and therefore could be useful in delaying the disease progression.  (+info)

The small heat shock protein alpha B-crystallin negatively regulates apoptosis during myogenic differentiation by inhibiting caspase-3 activation. (2/314)

Myoblasts respond to growth factor deprivation either by differentiating into multinucleated myotubes or by undergoing apoptosis; hence, the acquisition of apoptosis resistance by myogenic precursors is essential for their development. Here we demonstrate that the expression of the small heat shock protein alpha B-crystallin is selectively induced in C2C12 myoblasts that are resistant to differentiation-induced apoptosis, and we show that this induction occurs at an early stage in their differentiation in vitro. In contrast, the expression of several known anti-apoptotic proteins (FLIP, XIAP, Bcl-x(L)) was not altered during myogenesis. We also demonstrate that ectopic expression of alpha B-crystallin, but not the closely related small heat shock protein Hsp27, renders C2C12 myoblasts resistant to differentiation-induced apoptosis. Furthermore, we show that the myopathy-causing R120G alpha B-crystallin mutant is partly impaired in its cytoprotective function, whereas a pseudophosphorylation alpha B-crystallin mutant that mimics stress-induced phosphorylation is completely devoid of anti-apoptotic activity. Finally, we demonstrate that alpha B-crystallin negatively regulates apoptosis during myogenesis by inhibiting the proteolytic activation of caspase-3, whereas the R120G and pseudophosphorylation mutants are defective in this function. Taken together, our findings indicate that alpha B-crystallin is a novel negative regulator of myogenic apoptosis that directly links the differentiation program to apoptosis resistance.  (+info)

Orientation-dependent influence of an intergenic enhancer on the promoter activity of the divergently transcribed mouse Shsp/alpha B-crystallin and Mkbp/HspB2 genes. (3/314)

The mouse Shsp/alphaB-crystallin and Mkbp/HspB2 genes are closely linked and divergently transcribed. In this study, we have analyzed the contribution of the intergenic enhancer to Shsp/alphaB-crystallin and Mkbp/HspB2 promoter activity using dual-reporter vectors in transient transfection and transgenic mouse experiments. Deletion of the enhancer reduced Shsp/alphaB-crystallin promoter activity by 30- and 93-fold and Mkbp/HspB2 promoter activity by 6- and 10-fold in transiently transfected mouse lens alpha-TN4 and myoblast C2C12 cells, respectively. Surprisingly, inversion of the enhancer reduced Shsp/alphaB-crystallin promoter activity by 17-fold, but did not affect Mkbp/HspB2 promoter activity in the transfected cells. In contrast, enhancer activity was orientation-independent in combination with a heterologous promoter in transfected cells. Transgenic mouse experiments established the orientation dependence and Shsp/alphaB-crystallin promoter preference of the intergenic enhancer in its native context. The orientation dependence and preferential effect of the Shsp/alphaB-crystallin enhancer on the Shsp/alphaB-crystallin promoter provide an example of adaptive changes in gene regulation accompanying the functional diversification of duplicated genes during evolution.  (+info)

Retinal pigment epithelium is protected against apoptosis by alphaB-crystallin. (4/314)

PURPOSE: The degeneration of retinal pigment epithelial (RPE) cells is considered to be a crucial event in the pathophysiology of age-related macular degeneration (AMD). Cumulative oxidative damage has been implicated in the development of the changes seen in AMD. The present study was undertaken to evaluate the expression of the small heat shock protein alphaB-crystallin in the RPE in response to oxidative stress and to explore whether alphaB-crystallin expression confers an antiapoptotic cytoprotective effect on RPE cells. METHODS: Native human RPE cells from the macula and retinal periphery were analyzed by RT-PCR and Western blot analysis for expression of alphaB-crystallin. Monolayer cultures of human RPE cells were stressed by heat shock (42 degrees C for 20 minutes) or oxidant-mediated injury (50-300 micro M H(2)O(2) for 1 hour). Induction of alphaB-crystallin and the corresponding mRNA was assessed by Western and Northern blot analyses. To study the cytoprotective effect of alphaB-crystallin, human RPE cells were transfected with either a neomycin-selectable expression vector containing alphaB-crystallin cDNA or a control vector without alphaB-crystallin cDNA. Caspase-3 activity was determined by observing the cleavage of a colorimetric peptide substrate. Cell viability was quantified by combined propidium iodide and Hoechst 33342 staining. RESULTS: alphaB-crystallin is constitutively expressed in RPE under in vivo and in vitro conditions. Western blot analysis of freshly isolated RPE showed greater baseline expression levels in RPE derived from the macular area than in that from the more peripheral regions. Heat shock treatment and oxidative stress caused a significant increase in alphaB-crystallin mRNA and protein. Oxidant-mediated injury in RPE cells with baseline expression levels of alphaB-crystallin resulted in apoptotic cell death, as measured by caspase-3 activity, whereas RPE cells that had been stably transfected with alphaB-crystallin were more resistant to H(2)O(2)-induced cellular injury. CONCLUSIONS: alphaB-crystallin may function as a stress-inducible antiapoptotic protein in human RPE and is inducible by oxidative stress, a condition implicated in the pathogenesis of AMD. Overexpression of alphaB-crystallin may be an important mechanism for the RPE to prevent apoptotic cell death in response to cellular stress.  (+info)

The small heat-shock protein alpha B-crystallin promotes FBX4-dependent ubiquitination. (5/314)

AlphaB-crystallin is a small heat-shock protein in which three serine residues (positions 19, 45, and 59) can be phosphorylated under various conditions. We describe here the interaction of alphaB-crystallin with FBX4, an F-box-containing protein that is a component of the ubiquitin-protein isopeptide ligase SCF (SKP1/CUL1/F-box). The interaction with FBX4 was enhanced by mimicking phosphorylation of alphaB-crystallin at both Ser-19 and Ser-45 (S19D/S45D), but not at other combinations. Ser-19 and Ser-45 are preferentially phosphorylated during the mitotic phase of the cell cycle. Also alphaB-crystallin R120G, a mutant found to co-segregate with a desmin-related myopathy, displayed increased interaction with FBX4. Both alphaB-crystallin S19D/S45D and R120G specifically translocated FBX4 to the detergent-insoluble fraction and stimulated the ubiquitination of one or a few yet unknown proteins. These findings implicate the involvement of alphaB-crystallin in the ubiquitin/proteasome pathway in a phosphorylation- and cell cycle-dependent manner and may provide new insights into the alphaB-crystallin-induced aggregation in desmin-related myopathy.  (+info)

Mechanism of chaperone function in small heat-shock proteins. Phosphorylation-induced activation of two-mode binding in alphaB-crystallin. (6/314)

The consequences of alphaB-crystallin phosphorylation on its chaperone activity were investigated using a detailed analysis of the recognition and binding of destabilized T4 lysozyme (T4L) mutants by alphaB-crystallin phosphorylation mimics containing combinations of serine to aspartate substitutions. The T4L site-directed mutants were selected to constitute an energetic ladder of progressively destabilized proteins having similar structures in the folded state. alphaB-crystallin and its variants differentially recognize the T4L mutants, binding the more destabilized ones to a larger extent. Furthermore, the aspartate substitutions result in an increase in the extent of binding to the same T4L mutant and in the appearance of biphasic binding isotherms. The latter indicates the presence of two modes of binding characterized by different affinities and different numbers of binding sites. The transition to two-mode binding can also be induced by temperature or pH activation of the second mode. The similarity between the phosphorylation, pH, and temperature effects suggests a common structural origin. The location of the phosphorylation sites in the N-terminal domain and the hypothesized burial of this domain in the core of the oligomeric structure are consistent with a critical role for the destabilization of the quaternary structure in the process of recognition and binding by small heat-shock proteins.  (+info)

Morphological characterization of the Alpha A- and Alpha B-crystallin double knockout mouse lens. (7/314)

BACKGROUND: One approach to resolving some of the in vivo functions of alpha-crystallin is to generate animal models where one or both of the alpha-crystallin gene products have been eliminated. In the single alpha-crystallin knockout mice, the remaining alpha-crystallin may fully or partially compensate for some of the functions of the missing protein, especially in the lens, where both alpha A and alpha B are normally expressed at high levels. The purpose of this study was to characterize gross lenticular morphology in normal mice and mice with the targeted disruption of alpha A- and alpha B-crystallin genes (alpha A/BKO). METHODS: Lenses from 129SvEvTac mice and alpha A/BKO mice were examined by standard scanning electron microscopy and confocal microscopy methodologies. RESULTS: Equatorial and axial (sagittal) dimensions of lenses for alpha A/BKO mice were significantly smaller than age-matched wild type lenses. No posterior sutures or fiber cells extending to the posterior capsule of the lens were found in alpha A/BKO lenses. Ectopical nucleic acid staining was observed in the posterior subcapsular region of 5 wk and anterior subcapsular cortex of 54 wk alpha A/BKO lenses. Gross morphological differences were also observed in the equatorial/bow, posterior and anterior regions of lenses from alpha A/BKO mice as compared to wild mice. CONCLUSION: These results indicated that both alpha A- and alpha B-crystallin are necessary for proper fiber cell formation, and that the absence of alpha-crystallin can lead to cataract formation.  (+info)

Mimicking phosphorylation of alphaB-crystallin on serine-59 is necessary and sufficient to provide maximal protection of cardiac myocytes from apoptosis. (8/314)

AlphaB-crystallin (alphaBC), a small heat shock protein expressed in high levels in the heart, is phosphorylated on Ser-19, 45, and 59 after stress. However, it is not known whether alphaBC phosphorylation directly affects cell survival. In the present study, constructs were prepared that encode forms of alphaBC harboring Ser to Ala (blocks phosphorylation) or Ser to Glu (mimics phosphorylation) mutations at positions 19, 45, and 59. The effects of each form on apoptosis of cultured cardiac myocytes after hyperosmotic or hypoxic stress were assessed. Compared with controls, cells that expressed alphaBC with Ser to Ala substitutions at all three positions, alphaBC(AAA), exhibited more stress-induced apoptosis. Cells expressing either alphaBC(AAE) or (EEE) exhibited 3-fold less apoptosis than cells expressing alphaBC(AAA), indicating that phosphorylation of Ser-59 confers protection. alphaBC is known to bind to procaspase-3 and to decrease caspase-3 activation. Compared with cells expressing alphaBC(AAA), the activation of caspase-3 was decreased by 3-fold in cells expressing alphaBC(AAE). These results demonstrate that mimicking the phosphorylation of alphaBC on Ser-59 is necessary and sufficient to confer caspase-3 inhibition and protection of cardiac myocytes against hyperosmotic or hypoxic stress. These findings provide direct evidence that alphaBC(S59P) contributes to the cardioprotection observed after physiologically relevant stresses, such as transient hypoxia. Identifying the targets of alphaBC(S59P) will reveal important details about the mechanism underlying the cytoprotective effects of this small heat shock protein.  (+info)

The molecular chaperone alphaB-crystallin has emerged as a target for cancer therapy due to its expression in human tumors and its role in regulating tumor angiogenesis. alphaB-crystallin also reduces neuroinflammation, but its role in other inflammatory conditions has not been investigated. Here, we examined whether alphaB-crystallin regulates inflammation associated with tumors and ischemia. We found that CD45(+) leukocyte infiltration is 3-fold increased in tumors and ischemic myocardium in alphaB-crystallin-deficient mice. Notably, alphaB-crystallin is prominently expressed in CD11b(+) Gr-1(+) immature myeloid cells (IMCs), known as regulators of angiogenesis and immune responses, while lymphocytes and mature granulocytes show low alphaB-crystallin expression. alphaB-Crystallin deficiency results in a 3-fold higher accumulation of CD11b(+) Gr-1(+) IMCs in tumors and a significant rise in CD11b(+) Gr-1(+) IMCs in spleen and bone marrow. Similarly, we noted a 2-fold increase in CD11b(+) ...
Expression of small stress proteins (shsp) enhances the survival of mammalian cells exposed to heat or oxidative injuries. Recently, we have shown that the expression of shsp from different species, such as human hsp27, Drosophila hsp27 or human alphaB-crystallin protected murine L929 cells against …
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P. De Ronsard; Œuvres Poétiques, Vol. 1: Index Alphabétique Classic Reprint French Edition [Vaganay, Hugues] on. FREE shipping on qualifying offers. P. De Ronsard; Œuvres Poétiques, Vol. 1: Index Alphabétique Classic Reprint French Edition.
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INDIANAPOLIS - Unlike the three most common forms of breast cancer, triple negative breast cancer has no currently approved targeted therapies for treatment. That was one of the reasons it was chosen as a disease area of focus for the Indiana University Grand Challenge Precision Health Initiative, aimed to research and develop better treatments, cures or preventions, as quickly as possible.. Xiongbin Lu, PhD, a researcher in the Vera Bradley Foundation Center for Breast Cancer Research at Indiana University School of Medicine, working in collaboration with researchers from the University of Maryland, recently reported several important findings related to triple negative breast cancer and its treatment future, in the prestigious journal Nature Nanotechnology.. According to the paper now available online, TP53 is the most frequently mutated gene in triple negative breast cancer, meaning it is fueling the growth of this aggressive form of breast cancer. However, the problem with trying to target ...
Desmin-related myopathy is a subgroup of myofibrillar myopathy caused by mutations in desmin, CryAB, and other proteins that interact with the intermediate filaments. The pathology is characterized by myofibril disruption that appears to initiate at the Z-disk. Dislocation and aggregation of membranous organelles is observed as well as the accumulation of the mutant, misfolded desmin, and/or CryAB into insoluble aggregates, which gradually increase in the cytoplasm and are thought to eventually result in cell death.1-3 We previously showed that these aggregates may be classified as aggresomes, whose accumulation is often associated with neurodegenerative diseases caused by protein misfolding or unfolding.8 Our studies showed that the aggresomes present in the cardiomyocytes contain large concentrations of a toxic amyloid oligomer, which is typically found in many of the amyloid-based neurodegenerative diseases. The data thus link these cardiomyopathies to a broad class of amyloid-based ...
Desmin-related cardiomyopathy (DRC) belongs to the family of myofibrillar diseases characterized by the presence of toxic desmin-positive aggregates and degeneration at Z-disk structures. DRC has been linked to the mutations in the desmin, αBcrystallin (CryAB), or myotilin genes. Several human CryAB mutations cause multisystem disorders and experimental models of R120GCryAB in transgenic mice strikingly recapitulate similar phenotype observed in patients. In this context, we have generated the 450delACryAB linked to inheritable cataracts in neonatal rat cardiomyocytes and mice hearts. 450delACryAB formed aggregates or aggresomes, and recruited HSP25 into the insoluble fraction in the cardiomyocytes. However, heart-specific 450delA CryAB overexpression neither causes desmin-related cardiomyopathy nor exacerbates pressure overload-induced hypertrophy. To our knowledge, this is the first report of compensation to protein aggregates formation abrogates the development of cardiomyopathy and suggests ...
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TY - JOUR. T1 - Matrix metalloproteinase-1 induces cleavage of exogenous alphab-crystallin transduced by a cell-penetrating peptide. AU - Yang, Seung Won. AU - Lee, Seung Min. AU - Choi, Eun Young. AU - Lee, Kyung Hye. AU - Kim, Soo Hyuk. AU - Shin, Min Jeong. AU - Han, Ye Sun. AU - Kang, Seok Min. AU - Chung, Ji Hyung. PY - 2011/9/1. Y1 - 2011/9/1. N2 - Cell-penetrating peptides (CPPs), including TAT-CPP, have been used to deliver exogenous proteins into living cells. Although a number of proteins fused to TAT-CPP can be delivered into various cells, little is known about the proteolytic cleavage of TAT-fusion proteins in cells. In this study, we demonstrate that a small heat shock protein (sHSP), alphaB-crystallin (αB-crystallin), delivered by TAT-CPP is susceptible to proteolytic cleavage by matrix metalloproteinase-1 (MMP-1) in cardiac myoblast H9c2 cells. Recombinant TAT-αB-crystallin was efficiently transduced into H9c2 cells. For a few hours following protein transduction, generation of ...
Not surprisingly, the body can also suffer from reductive stress which frequently goes unmentioned. Reductive stress is achieved when the concentrations of reducing agents exceeds that of oxidizing agents. This is commonly encountered with the metabolism of ethanol. It is therefore possible for Health Fanatics to overdose on anti-oxidants and push the body into reductive stress instead of the oxidative stress they are steadfastly trying to avoid. Exercise can have positive or negative effects with regard to Redox effects on the body according to how it is performed. Exercise increases oxidative stress due to metabolic processes but when exercise is undertaken on a regular basis the body up-regulates its own anti-oxidant capacity to cope with the increased demand. This improved anti-oxidant activity clearly has additional benefits when we are not exercising but are under oxidative stress in other ways. Intermittent exercise will increase the oxidative stress burden on the body but is not ...
Accordingly, its important to look beyond the ostensibly obvious results like low AGE diets promote fat and weight loss. While this may be true, even the cursory analysis of the study results in todays SuppVersity short news indicates that the obvious conclusion that reducing AGEs would mechanistically reduce body fat is probably flawed. Rather than that people who avoid high AGE foods will necessary avoid foods with high energy density, consume less energy and lose weight in response to a reduction in energy intake - not in response to the reduced intake of AGEs. Problems like these are why it is important to look beyond the conclusions in the abstracts and / or come back to the SuppVersity regularly for short and in-depth analyses of the latest studies , Comment on Facebook ...
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Learn about Alexander Disease and other health conditions at HealtheTreatment. What is Alexander Disease? Compare Alexander Disease symptoms Alexander Disease treatments and . Share online in the health community.
TY - JOUR. T1 - P62 Stages an Interplay Between the Ubiquitin-Proteasome System and Autophagy in the Heart of Defense Against Proteotoxic Stress. AU - Su, Huabo. AU - Wang, Xuejun. PY - 2011/11/1. Y1 - 2011/11/1. N2 - As exemplified by desmin-related cardiomyopathy and myocardial ischemia/reperfusion injury, proteasome functional insufficiency plays an essential pathogenic role in the progression of cardiac diseases with elevated proteotoxic stress. Upregulation of p62/SQSTM1 and increased selective autophagy in cardiomyocytes may protect against proteotoxic stress in the heart. p62 may serve as a proteotoxic stress sensor, promote segregation and degradation of misfolded proteins by autophagy, and mediate the cross talk between the ubiquitin-proteasome system and autophagy.. AB - As exemplified by desmin-related cardiomyopathy and myocardial ischemia/reperfusion injury, proteasome functional insufficiency plays an essential pathogenic role in the progression of cardiac diseases with elevated ...
Alpha-crystallin B chain is a protein that in humans is encoded by the CRYAB gene. It is part of the small heat shock protein family and functions as molecular chaperone that primarily binds misfolded proteins to prevent protein aggregation, as well as inhibit apoptosis and contribute to intracellular architecture. Post-translational modifications decrease the ability to chaperone. Defects in this gene/protein have been associated with cancer and neurodegenerative diseases such as Alzheimers disease and Parkinsons disease. Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and ...
Alexander disease is a leukodystrophy, a group of more than 15 rare genetic disorders that cause progressive degeneration of the white matter of the brain due…
BACKGROUND: We studied a family including two half-siblings, sharing the same mother, affected by slowly progressive, adult-onset neurological syndromes. In spite of the diversity of the clinical features, characterized by a mild movement disorder with cognitive impairment in the elder patient, and severe motor-neuron disease (MND) in her half-brother, the brain Magnetic Resonance Imaging (MRI) features were compatible with adult-onset Alexanders disease (AOAD), suggesting different expression of the same, genetically determined, condition.METHODS: Since mutations in the alpha isoform of glial fibrillary acidic protein, GFAP-α, the only cause so far known of AOAD, were excluded, we applied exome Next Generation Sequencing (NGS) to identify gene variants, which were then functionally validated by molecular characterization of recombinant and patient-derived cells.RESULTS: Exome-NGS revealed a mutation in a previously neglected GFAP isoform, GFAP-ϵ, which disrupts the GFAP-associated ...
Alexander disease, a Leukodystrophy, is a progressive and fatal neurological disorder in which the destruction of white matter in the brain is accompanied by the formation of abnormal deposits known as Rosenthal fibers. Rosenthal fibers are aggregations of protein that occur in astrocytes, one of the so-called glial cells of the central nervous system. These aggregates are found occasionally in other disorders, but not with the abundance or particular distribution in the brain that occurs in Alexander disease.. The age of onset is quite variable, ranging from prenatal through the sixth decade. The most common classification divides patients into three categories based on age of onset, infantile (0-2 years), juvenile (2-12 years), and adult (,12 years). More recently, a different classification has been proposed, with only two categories of type I and type II, with the distinction hinging more on distribution of lesions and clinical presentation rather than age of onset (all type I cases being ...
A representation of NAD showing the change that occurs in the nicotinamide moiety (encircled) when NAD+ is reduced to NADH. In NAD+, the nicotinamide moiety has a planar structure, whereas it is puckered in NADH as shown in this animation (avi).. In facultative aerobic bacteria the catabolism of fuel molecules is associated with the reduction of NAD+ to NADH. During the transition to oxygen limited growth, an increased level of NADH builds up, as it is less efficiently reoxidized to NAD+ as a result of reduced aerobic respiration. These roles of NADH and NAD+ provide a link between energy homeostasis and gene regulation. In most Gram-positive bacteria a novel sensor of the NAD redox balance is present. This transcriptional repressor is called Rex (from redox; Figure 2). Transcription of Rex-repressed genes in Bacillus subtilis is activated when oxygen is limiting for aerobic respiration, and as a consequence the levels of free NADH increase. This leads to production of cytochrome bd (a ...
α-Crystallin is the principal lens protein which, in addition to its structural role, also acts as a molecular chaperone, to prevent aggregation and precipitation of other lens proteins. One of its two subunits, αB-crystallin, is also expressed in many non-lenticular tissues, and a natural missense mutation, R120G, has been associated with cataract and desminrelated myopathy, a disorder of skeletal muscles (Vicart et al., 1998, Nature Genet. 20:92-95). In the present study, real-time 1H NMR spectroscopy showed that the ability of R120G αB-crystallin to stabilize the partially folded, molten globule state of α- lactalbumin was significantly reduced in comparison with wild type αB-crystallin. The mutant showed enhanced interaction with, and promoted unfolding of reduced α- lactalbumin, while it showed limited chaperone activity for other target proteins. Using NMR spectroscopy, gel electrophoresis and mass spectrometry (MS) it was observed that, unlike the wild type protein, R120G αB-crystallin is
See the Rosenthal surname, family crest & coat of arms. Free Search. Uncover the Rosenthal surname history for the German Origin. What is the history of the last name Rosenthal?
Order Novelties online - Discover 770 Novelties from the Rosenthal brand environments and collections. Purchase your porcelain and more from the Rosenthal Porcelain Online Shop.
Order Novelties online - Discover 780 Novelties from the Rosenthal brand environments and collections. Purchase your porcelain and more from the Rosenthal Porcelain Online Shop.
ATGen operates in the Commercial Physical Research sector. ATGen is a Korea-based company principally engaged in developing and manufacturing reagent used for experimentation . The Companys product portfolio consists of recombination proteins including binding immunoglobulin protein, carboxy terminus of HSP70 interacting protein, BCL2-associated athanogene 2; monoclonal antibodies including 14-3-3 beta antibody, A crystallin A antibody , alpha-crystallin B and NK Vue Kit used for physical examination and diagnosis. The Company distributes its products within domestic market and to overseas markets.
The Rosenthal Malibu winery holds a variety of year-round Malibu wine tasting events. We offer special VIP access for members and a variety of wines online.
The Rosenthal Malibu winery holds a variety of year-round Malibu wine tasting events. We offer special VIP access for members and a variety of wines online.
Visit Healthgrades for information on Dr. Robert Rosenthal, MD Find Phone & Address information, medical practice history, affiliated hospitals and more.
Visit Healthgrades for information on Dr. Howard Rosenthal, DDS Find Phone & Address information, medical practice history, affiliated hospitals and more.
Listen free to Tom Rosenthal - The Pleasant Trees (Its OK, Going To be Wonderful and more). 6 tracks (16:49). Discover more music, concerts, videos, and pictures with the largest catalogue online at
Nikolai Rosenthal is the author of this article in the Journal of Visualized Experiments: תכנון ויישום מערכת עצבים בסימולציות לגו רובוטים
A young Southern woman of modest means suddenly finds herself thrust into New Yorks high society when she discovers that she is the illegitimate daughter of...
Purpose: : In our previous studies, we have demonstrated that alpha-crystallins can negatively regulate stress-induced apoptosis through suppression of the ERK-mediated pathway and activation of the AKT signaling pathway. In the present study, we present evidence to show that alpha-crystallins can regulate the ATR-p53 pathway to prevent UVA-induced apoptosis. Methods: : UVA was used to irradiate human lens epithelial cells stably expressing vector, alphaA, and alphaB. Western blot analysis was used for detection of ATR, CHK1/2 and p53 activation. Reporter gene activity assay was used to explore the transactivity of p53. Hoechst staining was used for apoptosis assay. Results: : Human lens epithelial cells expressing either alphaA- or alphaB-crystallin are substantially resistant to UVA-induced apoptosis. UVA-induces activation of ATR and CHK1/2 kinases to activate p53 in vector-transfected cells. However, in alphaA- or alphaB-crystallin-transfected cells, activation of ATR, CHK1/2 kinases and p53 ...
Alexander disease, also known as fibrinoid leukodystrophy, is a progressive and fatal neurodegenerative disease. It is a rare genetic disorder and mostly affects infants and children, causing developmental delay and changes in physical characteristics. Delays in development of some physical, psychological and behavioral skills; progressive enlargement of the head (macrocephaly), seizures, spasticity, and in some cases also hydrocephalus, idiopathic intracranial hypertension, and dementia. Alexander disease is a genetic disorder affecting the midbrain and cerebellum of the central nervous system. It is caused by mutations in the gene for glial fibrillary acidic protein (GFAP) that maps to chromosome 17q21. It is inherited in an autosomal dominant manner, such that the child of a parent with the disease has a 50% chance of inheriting the condition, if the parent is heterozygotic. However, most cases arise de novo as the result of sporadic mutations. Alexander disease belongs to leukodystrophies, a ...
Mycobacterium tuberculosis (Mtb) forms biofilms harbouring antibiotic-tolerant bacilli in vitro, but the factors that induce biofilm formation and the nature of the extracellular material that holds the cells together are poorly understood. Here we show that intracellular thiol reductive stress (TRS) induces formation of Mtb biofilms in vitro, which harbour drug-tolerant but metabolically active bacteria with unchanged levels of ATP/ADP, NAD(+)/NADH and NADP(+)/NADPH. The development of these biofilms requires DNA, RNA and protein synthesis. Transcriptional analysis suggests that Mtb modulates only ∼7% of its genes for survival in biofilms. In addition to proteins, lipids and DNA, the extracellular material in these biofilms is primarily composed of polysaccharides, with cellulose being a key component. Our results contribute to a better understanding of the mechanisms underlying Mtb biofilm formation, although the clinical relevance of Mtb biofilms in human tuberculosis remains ...
TY - BOOK. T1 - Genetic variation of alphaB-crystallin in multiple sclerosis, hypertrophic cardiomyopathy and dilated cardiomyopathy. AU - Støvring, Birgitte. PY - 2009. Y1 - 2009. M3 - Ph.D. thesis. BT - Genetic variation of alphaB-crystallin in multiple sclerosis, hypertrophic cardiomyopathy and dilated cardiomyopathy. PB - Roskilde Universitet. CY - Roskilde. ER - ...
Abstract: Small heat shock proteins form large cytosolic assemblies from an α-crystallin domain (ACD) flanked by sequence extensions. Mutation of a conserved arginine in the ACD of several human small heat shock protein family members causes many common inherited diseases of the lens and neuromuscular system. The mutation R120G in αB-crystallin causes myopathy, cardiomyopathy and cataract. We have solved the X-ray structure of the excised ACD dimer of human αB R120G close to physiological pH and compared it with several recently determined wild-type vertebrate ACD dimer structures. Wild-type excised ACD dimers have a deep groove at the interface floored by a flat extended bottom sheet. Solid-state NMR studies of large assemblies of full-length αB-crystallin have shown that the groove is blocked in the ACD dimer by curvature of the bottom sheet. The crystal structure of R120G ACD dimer also reveals a closed groove, but here the bottom sheet is flat. Loss of Arg120 results in rearrangement of ...
DNAJC21 (DnaJ heat shock protein family (Hsp40) member C21), Authors: Dessen P. Published in: Atlas Genet Cytogenet Oncol Haematol.
DNAJC4 (DnaJ heat shock protein family (Hsp40) member C4), Authors: Dessen P. Published in: Atlas Genet Cytogenet Oncol Haematol.
Health,...SALT LAKE CITY -- Antioxidants are widely considered an important defe...The findings published in the Aug. 10 issue of Cell indicate a new c...The protein alpha B-Crystallin termed a molecular chaperone normally...In a study of laboratory mice with failing hearts caused by mutant alp...,Reductive,stress,linked,to,heart,disease,medicine,medical news today,latest medical news,medical newsletters,current medical news,latest medicine news
The eleven students involved learned basic experimental design (with positive and negative controls), troubleshooting, a wide variety of laboratory techniques, and data collection, analysis and presentations skills. In addition, students learned to read and analyze scientific work from other labs since they are given scientific articles to read beginning on their first day in the lab. This project is in collaboration with Ivor Benjamin M.D., Ph.D. at the University of Utah, which allowed these students the unique opportunity of discussing their research with a larger audience once a month. In addition to intellectual enrichment, students learned to function as a team, with those more experienced (graduate students Kelsey Langston and Whitney Hayes) mentoring those who are new in the lab. As students progressed in their understanding of the project and mastery of basic laboratory skills, they become involved in planning our weekly group meeting, preparing figures for presentations/publications, ...
View mouse Hspb11 Chr4:107253593-107279938 with: phenotypes, sequences, polymorphisms, proteins, references, function, expression
A slowly progressive and ultimately fatal brain disorder that most commonly occurs in children. The infantile form of the disease is characterized by megalencephaly (an abnormally large head), seizures, spasticity and developmental retardation.…
Alexander disease (AxD) is a primary genetic disorder of astrocytes caused by dominant mutations in the gene encoding the intermediate filament (IF) protein GFAP. This disease is characterized by excessive accumulation of GFAP, known as Rosenthal fibers, within astrocytes.
Singh BN, Rao KS, Rao ChM (2010) Ubiquitin-proteasome-mediated degradation and synthesis of MyoD is modulated by alphaB-crystallin, a small heat shock protein, during muscle differentiation. Biochim Biophys Acta 1803, 288-99 20005263 ...
Emed, A.; Rosenthal, Móricz.; Grünfeld, József.; Spitzer, Sándor., 2003: Hungarian physicians in Vienna: Móric Rosenthal, József Grünfeld, Sándor Spitzer
Dr. Norman Rosenthal, MD is a psychiatry specialist in Rockville, MD. Dr. Rosenthal completed a residency at Ny State Psychology Institute. He currently practices at Capital Clinical Research Assocs.
Helianthus annuus hsp17.6 G1 protein: a small heat-shock protein from sunflower; amino acid sequence in first source; GenBank Z95153!/CCSVI_Society Dr. Alexander (endothelial biologist) now presenting MS and cerebral endothelial dysfunctionDr. Alexander: endothelium becomes sticky with shear: activates leukocytes into brain parenchyma Dr. Alexander: endothelial cells very sensitive to glycemic (glucose) and oxidative stressDr. Alexander: Interferon beta may have ...
Mikecz, K., Glant, T. T., Markovics, A., Rosenthal, K. S., Kurko, J., Carambula, R. E., Cress, S., Steiner, H. L. & Zimmerman, D. H., Jul 13 2017, In : Vaccine. 35, 32, p. 4048-4056 9 p.. Research output: Contribution to journal › Article ...
Read ratings and reviews for Dr. Randall Rosenthal who has 28 years of experience as a Obstetrician & Gynecologist in Newport, RI.
Rosenthal, A S., Determinant selection and macrophage function in genetic control of the immune response. (1978). Subject Strain Bibliography 1978. 3821 ...
Heat-stress induced alterations in localization of small heat shock proteins in mouse myoblasts: intranuclear lamin A/C speckles as target for ?B-crystallin and hsp ...
Complete information for HSPA2 gene (Protein Coding), Heat Shock Protein Family A (Hsp70) Member 2, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
WHAT fellow art thou, who darest thus to lie at thy ease in our presence, when all others, as thou seest, rise to do us homage? dost thou not know us?. Diog. I cannot say I do: but by the number of thy attendants, by the splendour of thy habit; but, above all, by the vanity of thy appearance, and the arrogance of thy speech, I conceive thou mayst be Alexander the son of Philip.. Alex. And who can more justly challenge thy respect, than Alexander, at the head of that victorious army, who hath performed such wonderful exploits, [1] and, under his conduct, hath subdued the world?. Diog. Who? why the tailor who made me this old cloke.. Alex. Thou art an odd fellow, and I have a curiosity to know thy name.. Diog. I am not ashamed of it: I am called Diogenes: a name composed of as many and as well-sounding syllables as Alexander.. Alex. Diogenes, I rejoice at this encounter. I have heard of thy name, and been long desirous of seeing thee; in which wish, since fortune hath accidentally favoured me, I ...
Gene target information for HSPA13 - heat shock protein family A (Hsp70) member 13 (human). Find diseases associated with this biological target and compounds tested against it in bioassay experiments.
Alexander disease results from gain of function mutations in the gene encoding glial fibrillary acidic protein (GFAP). At least eight GFAP isoforms have been described, however, the predominant alpha isoform accounts for approximately 90% of GFAP protein.
Chen X, Lin S, Liu Q, Huang J, Zhang W, Lin J, Wang Y, Ke Y, He H. Expression and interaction of small heat shock proteins (sHsps) in rice in response to heat stress. Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics, 2014, 1844 (4): 818-828. ...
At file:///home/bar/mysql-bzr/mysql-trunk-wl946/ based on revid:[email protected] 3365 Alexander Barkov 2011-05-18 WL#946: fixing some test failures. As the datatype of @@timestamp has changed from Sys_var_session_special (ulonglong based) to Sys_var_session_special_double (double bases), its not any more correct to use ~(time_t)0 as the upper limit parameter to constructor. modified: sql/ === modified file sql/ --- a/sql/ 2011-05-18 17:21:03 +0000 +++ b/sql/ 2011-05-18 19:59:19 +0000 @@ -2730,7 +2730,7 @@ static bool check_timestamp(sys_var *sel static Sys_var_session_special_double Sys_timestamp( timestamp, Set the time for this client, sys_var::ONLY_SESSION, NO_CMD_LINE, - VALID_RANGE(0, ~(time_t)0), BLOCK_SIZE(1), + VALID_RANGE(0, TIMESTAMP_MAX_VALUE + 1), BLOCK_SIZE(1), NO_MUTEX_GUARD, IN_BINLOG, ON_CHECK(check_timestamp), ON_UPDATE(update_timestamp), ON_READ(read_timestamp)); Attachment: [text/bzr-bundle] ...
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View Alexander Semeonovs MOOC Record on Class Central. Class Central profile pages makes it easy for you to learn about Alexander Semeonovs interests in MOOCs.
Smenes, Benedikte Therese; Pettersen, Øystein; Stenseth, Roar; Karlsen, Øystein; Wahba, Alexander. (2017) Evaluering av phase of care mortality analysis og failure to rescue i en Norsk thoraxkirurgisk klinikk. Vitenskapelige forhandlinger / De norske kirurgiske foreninger. vol. 93. ...
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Find great deals for Thermo-Poroelasticity and Geomechanics by Alexander Suvorov, A. P. S. Selvadurai (Hardback, 2016). Shop with confidence on eBay!
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Aleksandr Blok (es); Александр Александрович Блок (kk-kz); Alexander Blok (en-gb); Александър Блок (bg); الیگزینڈر بلوک (pnb); الکساندربلوک (ur); Aleksandr Blok (sv); Блок Олександр Олександрович (uk); 亞歷山大·勃洛克 (zh-hant); 亚历山大·勃洛克 (zh-cn); Aleksandr Blok (uz); Александр Александрович Блок (kk); Aleksandro Blok (eo); Александар Блок (mk); আলেক্সান্দ্‌র ব্লক‌ (bn); Alexandre Blok (fr); Aleksander Aleksandrovič Blok (hr); अलेक्झांडर ब्लॉक (mr); Aleksander Aleksandrowič Blok (hsb); Aleksandr Aleksandrovich Blok (vi); الەكساندر الەكساندروۆىيتش بلوك (kk-arab); Aleksandrs Bloks (lv); Александар Блок (sr); Aleksandr Blok (pt-br); 亚历山大·勃洛克 (zh-sg); Александр Александрович Блок (kk-cyrl); Aleksandr ...
How It Ends (dir. David M. Rosenthal, 2018) - After a mysterious event sends the world into chaos, two men set out on a desperate drive to find their missing family.VerdictHow It Ends itself is as much of a disappointment as how it actually ends.ReviewAn unsettling disruption cuts short a call between Will (Theo James)…
Staub E, Fiziev P, Rosenthal A, Hinzmann B; , Bioessays 2004;26:567-581.: Insights into the evolution of the nucleolus by an analysis of its protein domain repertoire. PUBMED:15112237 EPMC:15112237. ...
Lewden C, May T, Rosenthal E, Burty C, Bonnet F, Costagliola D, et al. Changes in causes of death among adults infected by HIV between 2000 and 2005: The Mortalité 2000 and 2005 surveys (ANRS EN19 and Mortavic). J Acquir Immune Defic Syndr. 2008 Aug 15;48(5):590-8 ...
Lewden C, May T, Rosenthal E, Burty C, Bonnet F, Costagliola D, et al. Changes in causes of death among adults infected by HIV between 2000 and 2005: The Mortalité 2000 and 2005 surveys (ANRS EN19 and Mortavic). J Acquir Immune Defic Syndr. 2008 Aug 15;48(5):590-8 ...
Ilena Rosenthal used Tim Bolens defamatory posts to target Terry Polevoy, MD for destruction. Its now in the hands of the California courts for final disposition.
Alpha-crystallin A chain is a protein that in humans is encoded by the CRYAA gene. Crystallins are separated into two classes: ... Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins ... Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is ... the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an ...
... the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Alpha B chain crystallins (αBC) can be induced by heat shock, ... Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. These ... Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is ... Alpha-crystallin B chain is a protein that in humans is encoded by the CRYAB gene. It is part of the small heat shock protein ...
... alpha-crystallin a chain MeSH D12.776.306.366.100.300 - alpha-crystallin b chain MeSH D12.776.306.366.300.100 - beta-crystallin ... beta-crystallin b chain MeSH D12.776.331.199.750.500 - succinate dehydrogenase MeSH D12.776.377.715.085.050 - alpha 1- ... immunoglobulin heavy chains MeSH D12.776.377.715.548.705.500.350 - immunoglobulin alpha-chains MeSH D12.776.377.715.548.705. ... cholesterol 7 alpha-hydroxylase MeSH D12.776.422.220.453.915.212 - cholesterol side-chain cleavage enzyme MeSH D12.776.422.220. ...
Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as ... "Autosomal dominant cerulean cataract is associated with a chain termination mutation in the human beta-crystallin gene CRYBB2 ... Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four ... Beta-crystallin B2 is a protein that in humans is encoded by the CRYBB2 gene. Crystallins are separated into two classes: taxon ...
... heavy chain 14 ECM1 (1q21) EDEM3: ER degradation enhancing alpha-mannosidase like protein 3 EGLN1 (1q42) ENAH (1q42) ESRRG ( ... Crystallin zeta CYP4B1 (1p33) CYR61 (1p22) DBT: dihydrolipoamide branched chain transacylase E2 DCLRE1B: DNA cross-link repair ... 2-alpha-mannosidase IB MEAF6: MYST/ESA1 associated factor 6 MECR: Trans-2-enoyl-CoA reductase, mitochondrial MFAP2: ... C-4 to C-12 straight chain ACTL8: Actin-like 8 ADGRL2 (1p31.1): adhesion G protein-coupled receptor L2 ADPRHL2: Poly(ADP-ribose ...
... rho crystallin, and many others. All possess a similar structure, with a beta-alpha-beta fold characteristic of nucleotide ... Some proteins of this family contain a potassium channel beta chain regulatory domain; these are reported to have ... The fold comprises a parallel beta-8/alpha-8-barrel, which contains a novel NADP-binding motif. The binding site is located in ...
DNAH14 encoding protein Dynein, axonemal, heavy chain 14. *ECM1 (1q21). *EDEM3: ER degradation enhancing alpha-mannosidase like ... COL11A1: collagen, type XI, alpha 1. *CPT2: carnitine palmitoyltransferase II. *CRYZ: Crystallin zeta ... ACADM: acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain ... DBT: dihydrolipoamide branched chain transacylase E2. *DCLRE1B ...
Bsibsi M, Holtman IR, Gerritsen WH, Eggen BJ, Boddeke E, van der Valk P, van Noort JM, Amor S (2013). "Alpha-B-Crystallin ... free light chains (FLC). Several authors have reported that they are comparable or even better than oligoclonal bands. Multiple ...
... alpha 1 Collagen, type XIX, alpha 1 Collagen, type XXV, alpha 1 Collagen, type XXVII, alpha 1 Crystallin, beta A1 Cyclic ... DP alpha 1 Major histocompatibility complex, class II, DQ alpha 1 Myosin light chain A1, an actin-binding protein NADH ... alpha 1 collagen, type II, alpha 1 Collagen, type III, alpha 1 Collagen, type IV, alpha 1 Collagen, type V, alpha 1 Collagen, ... type VI, alpha 1 Collagen, type VII, alpha 1 Collagen, type VIII, alpha 1 Collagen, type IX, alpha 1 Collagen, type X, alpha 1 ...
Shum WK, Maleknia SD, Downard KM (September 2005). "Onset of oxidative damage in alpha-crystallin by radical probe mass ... The rate or level of oxidation at the reactive amino acid side chains (Met, Cys, Trp, Tyr, Phe, His, Pro and Leu) provides a ... The mechanisms of side chain oxidation were explored by performing the radiolysis reactions in 18O-labeled water. A critical ... Downard KM, Kokabu Y, Ikeguchi M, Akashi S (November 2011). "Homology-modelled structure of the βB2B3-crystallin heterodimer ...
The N-terminal domain I (aa ~1-195) contains attachment sites for HS chains. Although HS chains are not required for correct ... To this end, a transgenic mouse line was created expressing porcine TGF-β1 under the lens-specific αA-crystallin promoter and ... In a mouse model of Alzheimer's plaque formation, IL-1-alpha effects an increase in perlecan expression in response to brain ... Domain V also has attachment sites for HS/CS chains. Thus, perlecan core protein and HS chains could modulate matrix assembly, ...
The cause is lens hardening by decreasing levels of alpha-crystallin, a process which may be sped up by higher temperatures. ... The authors propose that mtDNA mutations lead to respiratory-chain-deficient cells and thence to apoptosis and cell loss. They ... the Growth hormone/Insulin-like growth factor 1 signalling pathway the activity levels of the electron transport chain in ...
COL11A1 collagen type XI alpha 1 chain [ Homo sapiens (human) ]». Gene. NCBI. 6. juni 2017. Henta 26. juni 2017.. ... CRYZ crystallin zeta [ Homo sapiens (human) ]». Gene. NCBI. 4. juni 2017. Henta 26. juni 2017.. ... MAN1A2 mannosidase alpha class 1A member 2 [ Homo sapiens (human) ]». Gene. NCBI. 6. juni 2017. Henta 26. juni 2017.. ... DBT dihydrolipoamide branched chain transacylase E2 [ Homo sapiens (human) ]». Gene. NCBI. 6. juni 2017. Henta 26. juni 2017.. ...
The propeptide region has an open-sandwich antiparallel-alpha/antiparallel-beta fold, with two alpha-helices and four beta- ... is a double-chain inhibitor consisting of an 11-residue and a 41-residue chain. The Carboxypeptidase inhibitor I68 family ... The yeast killer toxin structure was thought to be a precursor of the two-domain beta gamma-crystallin proteins, because of its ... It forms an alpha-helical domain that runs through the substrate-binding site, preventing access. Removal of this region by ...
Also in the 1970s, physicists Tanaka & Benedek at MIT identified phase-separation behaviour of gamma-crystallin proteins from ... chains linked by covalent bonds), which he termed 'macromolecules' to distinguish them from colloidal clusters of lower ... Robert Corey and Herman Branson correctly proposed the alpha helix and beta sheet as the primary structural motifs in protein ... "Configurations of Polypeptide Chains With Favored Orientations Around Single Bonds: Two New Pleated Sheets". Proceedings of the ...
The translation of reticulocyte 9S RNA in frog oocytes gives rise to alpha and beta globin chains. Communication to FEBS ... Berns, A. J. M.; Kraaikamp, M. Van; Bloemendal, H.; Lane, C. D. (1972). "Calf Crystallin Synthesis in Frog Cells: The ...
Domain 2 contains one small beta sheet, nine alpha helices, and the carboxyl terminus. Three of the nine alpha helices on one ... δ-crystallins are the major structural eye lens water-soluble proteins of most birds, reptiles, and some other vertebrates. ... ASL is composed of four identical monomers; each monomer consisting of a single polypeptide chain between 49 and 52 kDa, ... Two dimers then associate by way of alpha helix, one from each monomer, to form a central 20-helix core. The association of all ...
Silva, R.A.G.D., *Nguyen, J.Y., and Decatur, S.M. (2002) "Probing the Effects of Side Chains on the Conformation and Stability ... "Formation of Amyloid Fibrils in Vitro by Human γd-crystallin and its Isolated Domains," Molecular Vision 14:81-89. Decatur, S.M ... "Two dimensional infrared measurements of the coupling of the amide modes of an alpha helix," Chemical Physics Letters 382: 586- ... "Twodimensional IR Spectroscopy and Segmental 13C Labeling reveals the Domain Structure of Human γδ-crystallin amyloid fibrils ...
These compounds are thought to act as bidentate nucleophiles that attack the adjacent carbonyls in the alpha-dicarbonyl ... and crystallin of the eyes. Covalent protein cross-links irreversibly link proteins together in the ECM of tissues. Glucosepane ... This then condenses with the arginine side chain to yield glucosepane in nucleophilic addition-elimination reactions of the ... such as collagen in the skin and crystallin in the eyes. Skin collagen, for instance, has a half-life of fifteen years. Because ...
... cholesterol side-chain cleavage enzyme MeSH D08.244.453.915.400 - 25-hydroxyvitamin d3 1-alpha-hydroxylase MeSH D08.244.453.915 ... delta-crystallins MeSH D08.811.520.232.400 - ammonia-lyases MeSH D08.811.520.232.400.200 - aspartate ammonia-lyase MeSH D08.811 ... cholesterol side-chain cleavage enzyme MeSH D08.811.682.690.708.170.915.400 - 25-hydroxyvitamin d3 1-alpha-hydroxylase MeSH ... cholesterol 7 alpha-hydroxylase MeSH D08.811.682.690.708.783.212 - cholesterol side-chain cleavage enzyme MeSH D08.811.682.690. ...
... and aspartate side-chains, with a glutamate side-chain playing a secondary role. These side-chains, to be specific Arg359, ... and Asp477 forms hydrogen bonds with the alpha hydroxyl group on the substrate, where it works to effectively bind the ... in the mammalian cornea by the stromal keratocytes and epithelial cells and is reputed to be one of the corneal crystallins. ... To be specific, the His 263 and His30 side-chains form hydrogen bonds to the aldehyde end of the substrate, which is deepest ...
... alpha and gamma-endorphin, bombesin Thyroid gland cells Thyroid epithelial cell Parafollicular cell Parathyroid gland cells ... Intermediate skeletal muscle cell Nuclear bag cell of muscle spindle Nuclear chain cell of muscle spindle Myosatellite cell ( ... Medium spiny neurons Astrocytes Oligodendrocytes Ependymal cells Tanycytes Pituicytes Anterior lens epithelial cell Crystallin- ... Parathyroid chief cell Oxyphil cell Pancreatic islets (islets of Langerhans) Alpha cell (secretes glucagon) Beta cell (secretes ...
Once a protein has been tagged with one ubiquitin molecule, additional rounds of ubiquitination form a polyubiquitin chain that ... Alpha crystallin. *Clusterin. *Survival of motor neuron *SMN1. *SMN2. Protein targeting. *Signal peptide ...
Ecroyd H, Carver JA (January 2009). "Crystallin proteins and amyloid fibrils". Cellular and Molecular Life Sciences. 66 (1): 62 ... Badar T, D'Souza A, Hari P (2018). "Recent advances in understanding and treating immunoglobulin light chain amyloidosis". ... "Inclusion formation and neuronal cell death through neuron-to-neuron transmission of alpha-synuclein". Proceedings of the ... In immunoglobulin light chain amyloidosis (AL amyloidosis), chemotherapy can be used to lower the number of the blood cells ...
C-terminal domain - rich in alpha helical structure acts as a 'lid' for the substrate binding domain. The helical subdomain ... When ATP is hydrolyzed to ADP the binding pocket of Hsp70 closes, tightly binding the now-trapped peptide chain. Further ...
Chen D, Li M, Luo J, Gu W (April 2003). "Direct interactions between HIF-1 alpha and Mdm2 modulate p53 function". The Journal ... Bálint E, Bates S, Vousden KH (July 1999). "Mdm2 binds p73 alpha without targeting degradation". Oncogene. 18 (27): 3923-9. doi ... Long-chain-fatty-acid-CoA ligase. 6.3: Carbon-Nitrogen. *Glutamine synthetase. *Ubiquitin ligase *Cullin ...
"Monitoring the prevention of amyloid fibril formation by alpha-crystallin. Temperature dependence and the nature of the ... Oligomerization is a chemical process that converts individual molecules into a chain consisting of a finite number of ... Intra-cellular deposits of tau protein are also seen in the disease, and may also be implicated, as has aggregation of alpha ... leading to a chain reaction akin to a prion infection. The oligomers are toxic to nerve cells. The other protein implicated in ...
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
... help/chain target=_top>More...,/a>,/p>ChainiPRO_0000125906. 1 - 175. Alpha-crystallin B chainAdd BLAST. 175. ... IPR037882, ACD_alphaB-crystallin. IPR001436, Alpha-crystallin/sHSP_animal. IPR003090, Alpha-crystallin_N. IPR008978, HSP20- ... IPR037882, ACD_alphaB-crystallin. IPR001436, Alpha-crystallin/sHSP_animal. IPR003090, Alpha-crystallin_N. IPR008978, HSP20- ... "The amino-acids sequence of the alphaB2 chain of bovine alpha-crystallin.". van der Ouderaa F.J., de Jong W.W., Hilderink A., ...
Alpha-crystallin A2 chainAdd BLAST. 173. ChainiPRO_0000444508. 1 - 172. Alpha-crystallin A2(1-172)By similarity. ,p>Manually ... IPR002068, A-crystallin/Hsp20_dom. IPR001436, Alpha-crystallin/sHSP_animal. IPR012274, Alpha-crystallin_A. IPR003090, Alpha ... IPR002068, A-crystallin/Hsp20_dom. IPR001436, Alpha-crystallin/sHSP_animal. IPR012274, Alpha-crystallin_A. IPR003090, Alpha ... Chain Alpha-crystallin A2 chain:. Molecular mass is 20029 Da. Determined by ESI. With 1 phosphate group.1 Publication. Manual ...
What is alpha crystallin B chain? Meaning of alpha crystallin B chain medical term. What does alpha crystallin B chain mean? ... Looking for online definition of alpha crystallin B chain in the Medical Dictionary? alpha crystallin B chain explanation free ... redirected from alpha crystallin B chain) CRYAB. A gene on chromosome 11q22.3-q23.1 that encodes alpha crystallin B, a protein ... Alpha crystallin B chain , definition of alpha crystallin B chain by Medical dictionary https://medical-dictionary. ...
Background Mutations in αB-crystallin result in proteotoxic cardiomyopathy with desmin mislocalization to protein aggregates. ... alpha-Crystallin B Chain / genetics* * alpha-Crystallin B Chain / metabolism Substances * Basic Helix-Loop-Helix Leucine Zipper ... Methods and Results Mice overexpressing the R120G mutant of αB-crystallin in cardiomyocytes ( Myh6-Cry ABR 120G) were subjected ... Cry ABR 120G-expressing hearts demonstrated increased interaction of desmin with αB-crystallin and reduced interaction with ...
Rabbit polyclonal Alpha B Crystallin (phospho S19) antibody validated for WB, IHC, ICC/IF and tested in Human, Mouse and Rat. ... Alpha crystallin B chain antibody. *Alpha(B)-crystallin antibody. *Alpha-crystallin B chain antibody ... Lane 1 : phospho-alpha-B crystallin (Ser19). Lane 2 : unphosphorylated alpha-B crystallin. Observed band size : 20 kDa (why is ... Anti-Alpha B Crystallin (phospho S19) antibody. See all Alpha B Crystallin primary antibodies. ...
Rabbit polyclonal alpha A Crystallin/CRYAA antibody. Validated in WB, IHC, BL, sELISA and tested in Mouse, Rat, Cow. Cited in ... O-glycosylated; contains N-acetylglucosamine side chains.. Deamidation of Asn-101 in lens occurs mostly during the first 30 ... Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products--alpha-A and alpha-B. There are ... Biochimica et Biophysica Acta 1991 v1080 p173-180) describes the alpha-A crystallin and how it is split into alpha-A1 and alpha ...
Mouse Monoclonal Anti-AlphaA Crystallin/CRYAA Antibody (1H3.B8). Validated: WB, ELISA, IB, ICC/IF. Tested Reactivity: Human. ... crystallin, alpha A. *Heat shock protein beta-4. *HSPB4. *HSPB4alpha-crystallin A chain ... Does not cross-react with aB-crystallin, Beta L-crystallin, BH- crystallin, gamma-crystallin, HSP25, HSP27 or HSP47 proteins. ... analysis of Bovine tissue lysate showing detection of Alpha A Crystallin protein using Mouse Anti-Alpha A Crystallin Monoclonal ...
... alpha A (CRYAA) Protein (His tag). Species: Sheep (Ovine). Source: Yeast. Order product ABIN1630960. ... Crystallin, alpha A (CRYAA) Background Recommended name: Alpha-crystallin A chain Cleaved into the following chain: 1.. Alpha- ... Crystallin, alpha A (CRYAA) (AA 1-173) protein (His tag) Crystallin, alpha A (CRYAA) (AA 1-173) protein (His tag). Details for ... alpha-crystallin A chain-like * crystallin alpha A S homeolog * alpha-crystallin A chain ...
PREDICTED: alpha-crystallin B chain isoform X1 [Mus musculus]. 175 aa protein ...
L-lactate dehydrogenase B chain; CRYAB: Alpha-crystallin B chain; YBX1: Nuclease-sensitive element-binding protein 1; PRKDC: ...
We identified a previously reported oligomerization disrupting mutation, c.62G > A (p.R21Q), in the Crystallin alpha A (CRYAA) ... The work strengthens the genotype-phenotype correlations between this functional mutation in the crystallin alpha A (CRYAA) ... PCR (polymerase chain reaction) primers to amplify coding and untranslated regions of the 51 genes were designed with the Ion ... Andley UP, Song Z, Wawrousek EF, Fleming TP, Bassnett S. Differential protective activity of alpha A- and alpha B-crystallin in ...
Recombinant Protein and Alpha-crystallin Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are ... Alpha-crystallin A chain. Alpha-crystallin A chain ELISA Kit. Alpha-crystallin A chain Recombinant. Alpha-crystallin A chain ... Alpha-crystallin B chain. Alpha-crystallin B chain ELISA Kit. Alpha-crystallin B chain Recombinant. Alpha-crystallin B chain ... Alpha-crystallin. Alpha-crystallin ELISA Kit. Alpha-crystallin Recombinant. Alpha-crystallin Antibody. Also known as Alpha- ...
alpha-Crystallin B Chain / genetics * alpha-Crystallin B Chain / physiology Substances * Antioxidants ...
alpha-Crystallin B Chain - physiology. -. dc.title. Identification of tumor suppressive activity by irradiation microcell- ... The association of alpha B-crystallin (CRYAB), a gene identified as being differentially expressed by gene profiling of NPC and ... The association of alpha B-crystallin (CRYAB), a gene identified as being differentially expressed by gene profiling of NPC and ... Alpha B-crystallin (CRYAB). Chromosome 11q. Irradiation microcell-mediated chromosome transfer. Microcell hybrid. ...
Alpha-crystallin A chain Show on y-axis - References (HTP + LTP). References (LTP). References (HTP). ...
crystallin alpha A. LOC102724652 43169.041. 21p12. alpha-crystallin A chain. LINC00322 43322.209. 21q22.3. long intergenic non- ... collagen type VI alpha 1 chain. COL6A2 46098.119. 21q22.3. collagen type VI alpha 2 chain. ...
McKenna, M. C. (1992). The alpha crystallin A chain of the eye lens and mammalian phylogeny. Ann. Zool. Fenn. 28: 349-360. ...
The maximal expression of the molecular chaperone, alpha crystallin B chain, occurs during the secretory phase. In view of ... and alpha crystallin B chain. The expression of Hsp27, Hsp60, and the constitutive form of Hsp70 (Hsc70) shows a sharp increase ...
Publications] Iwaki, A. Iwaki, T., Goldman, J. E., Liem, R. K. H.: Multiple mRNAs of rat brain alpha-crystallin B-chain result ... Thus, the accumulation of Alpha B-crystallin appears to be part of the repertoire of reactive processes of CNS glial cells and ... Publications] Iwaki,A.: Multiple mRNAs of rat brain α-crystallin B-chain result from alternative transcriptional initiation. ... Publications] Iwaki,T.: Immunohistochemical demonstration of alpha B-crystallin in hamartomas of tuberous sclerosis. Am.J. ...
Alpha-crystallin A chain is a protein that in humans is encoded by the CRYAA gene. Crystallins are separated into two classes: ... Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins ... Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is ... the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an ...
... the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Alpha B chain crystallins (αBC) can be induced by heat shock, ... Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. These ... Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is ... Alpha-crystallin B chain is a protein that in humans is encoded by the CRYAB gene. It is part of the small heat shock protein ...
Tandem mass spectrometry was applied both to ions of a tryptic fragment and intact protein of bovine alpha-crystallin A chain ... The [M + 19H](19+) to [M + 11H](11+) charge states of both phosphorylated and unphosphorylated bovine alpha-crystallin A chain ... A tryptic digest of the bovine alpha-crystallin A chain yielded a phosphopeptide containing one missed cleavage site. The ... Bovine alpha-crystallin A chain ANALYTICAL CHEMISTRY Hogan, J. M., Pitteri, S. J., McLuckey, S. A. 2003; 75 (23): 6509-6516 ...
Rat A crystallin A Monoclonal Antibody (Clone c9F2)-NP_000385 (MBS200007) product datasheet at MyBioSource, Primary Antibodies ... Alpha-crystallin A chain; Heat shock protein beta 4; Acry-1; Alpha crystallin A chain; CRYA 1; CRYA1; CRYAA; Crystallin Alpha 1 ... Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins ... This antibody is not shown cross-activity about Crystallin alpha B. A : Crystallin-alpha A recombinant protein B : Crystallin- ...
2470619 - Alpha b-crystallin is expressed in kidney epithelial cell lines and not in fibroblasts.. ... 18987199 - Glial chain migration requires pioneer cells.. 22775509 - Influence of stem-cell cycle time on accelerated re- ...
SDS-PAGE analysis of UV-A-exposed and unexposed WT αB-crystallin and its three deamidated mutant species. After UV-A-exposure ... alpha-Crystallin B Chain/chemistry*/isolation & purification/metabolism*. Minor. *Amino Acid Sequence ... The WT alphaB-crystallin and its three deamidated species were exposed to UV-A light (320-400 nm) at intensities of 20 or 50 J/ ... The WT alphaB-crystallin and its three deamidated species were exposed to UV-A light (320-400 nm) at intensities of 20 or 50 J/ ...
Hsp90s such as 1am1, Hsp100s and small heat shock proteins like (alpha)-crystallin proteins. Various classes of molecular ... chaperones cooperate for the folding of the nascent polypeptide chains in the cyotplasm [1]. See also Heat Shock Proteins. ...
Intracellular degradation of alpha-crystallin: fractionation and characterization of degraded alpha A-chains. Eur J Biochem. ... Human alpha-crystallin. I: the isolation and characterization of newly synthesized alpha-crystallin. Invest Ophthalmol Vis Sci. ... de Jong WW, Terwindt EC, Bloemendal H. The amino acid sequence of the A chain of human alpha-crystallin. FEBS Lett. 1975;58:310 ... Kamei A, Iwase H, Masuda K. Cleavage of amino acid residue(s) from the N-terminal region of alpha A- and alpha B-crystallins in ...
... myoglobins and alpha-crystallins (a and b chains) on the y axis and the various organisms in phylogenetic order on X axis ... In fact, myoglobins and alpha-crystallins are composed by "alpha-helices" (all-alpha class) and "beta-strands" (all-beta class ... In details, we have evaluated the GC content in sirtuins, myoglobins and alpha-crystallins and plotted the value of GC content ... We have chosen to compare sirtuin sequences with those of myoglobins and alpha-crystallins; firstly because these are well ...
The KO and KOTg samples were devoid of αB-crystallin as expected. WT samples showed higher amounts of αB-crystallin than WTTg ... The KO and KOTg samples were devoid of αB-crystallin as expected. WT samples showed higher amounts of αB-crystallin than WTTg ... We hypothesized that αB-crystallin protects cells against Aβ toxicity. To test this, we crossed αB-crystallin/HspB2 deficient ( ... We hypothesized that αB-crystallin protects cells against Aβ toxicity. To test this, we crossed αB-crystallin/HspB2 deficient ( ...
  • A gene on chromosome 11q22.3-q23.1 that encodes alpha crystallin B, a protein induced by heat shock that belongs to the small heat shock protein (sHSP, or HSP20) family. (
  • Background Mutations in αB-crystallin result in proteotoxic cardiomyopathy with desmin mislocalization to protein aggregates. (
  • Methods and Results Mice overexpressing the R120G mutant of αB-crystallin in cardiomyocytes ( Myh6-Cry ABR 120G) were subjected to IF or ad-lib feeding, or transduced with adeno-associated virus- TFEB or adeno-associated virus-green fluorescent protein after development of advanced proteotoxic cardiomyopathy. (
  • Cry ABR 120G-expressing hearts demonstrated increased interaction of desmin with αB-crystallin and reduced interaction with chaperone protein, HSPB 8, compared with wild type, which was reversed by both IF and TFEB transduction. (
  • Alpha-Crystallin comprises 40% of total lens protein composition. (
  • It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. (
  • AlphaB-crystallin, a major lens protein, is found in the central nervous system (CNS) and is a major protein component of Rosenthal fibers (RF), intracytoplasmic inclusions within astrocytes. (
  • Sequencing of the promoter and coding regions of the alphaB-crystallin genes in two patients revealed them to be of normal sequence, suggesting the accumulation of alphaB-crystallin in those brains is not due to any abnormality of the primary structure of the protein. (
  • Publications] Tomokane,N.: 'Rosenthal fibers share epitopes with αB-crystallin,glial fibrillary acidic protein,and ubiquitin,but not with vimentin.Immunoelectron microscopy with colloidal gold. (
  • Alpha-crystallin A chain is a protein that in humans is encoded by the CRYAA gene. (
  • Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. (
  • Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) family. (
  • alpha-crystallins, a major protein of the ocular lens, are thought to play a role in maintaining lens transparency, which are composed of two gene products alpha-A and alpha-B, for acidic and basic, respectively. (
  • This antibody is not shown cross-activity about Crystallin alpha B. A : Crystallin-alpha A recombinant protein B : Crystallin-alpha B recombinant protein C : Mouse eye lysates The Cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Crystallin alpha A antibody (1:3000). (
  • Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. (
  • Far-UV CD spectral analyses showed almost no changes among WT and deamidated species on UV-A-exposure except that the alphaB-Asn146Asp mutant protein showed maximum changes in the random coil structure relative to WT alphaB-crystallin and two other deamidated proteins. (
  • Molecular chaperones protect cells from the deleterious effects of protein misfolding and aggregation.We observed that the overall content of αB-crystallin, a small heat shock protein molecular chaperone, decreased in AD model mice in an age-dependent manner.Our results highlight a synergistic effect of combining chaperone deficiency in a transgenic mouse model for AD underscoring an important role for chaperones in protein misfolding diseases. (
  • We observed that the overall content of αB-crystallin, a small heat shock protein molecular chaperone, decreased in AD model mice in an age-dependent manner. (
  • To test this, we crossed αB-crystallin/HspB2 deficient (CRYAB⁻/⁻HSPB2⁻/⁻) mice with AD model transgenic mice expressing mutant human amyloid precursor protein. (
  • These findings demonstrate that the role of α-crystallins in lens development is conserved from mammals to zebrafish and set the stage for using the embryonic lens as a model system to test mechanistic aspects of α-crystallin chaperone activity and to develop strategies to fine-tune protein-protein interactions in aging and cataracts. (
  • A missense mutation in alpha B-crystallin that changes proline 20 to an arginine leads to diminished anti-apoptotic activity compared with the native protein. (
  • The αA-Y118D protein prevented nonspecific protein aggregation more effectively than wild-type αA-crystallin. (
  • Alpha-crystallin is the major protein of vertebrate eye lens, and plays a structural role in maintaining the lens transparency and proper refractive index. (
  • Both αA- and αB-crystallins are members of the small heat shock protein (sHSP) family. (
  • Alpha B-Crystallin (CRYAB) is purported to be a metastasis suppressor protein, and lack or lower CRYAB expression is a prognostic biomarker for several types of cancer, such as that of the prostate and head and neck. (
  • The well-characterized small heat-shock protein, αB-crystallin, acts as a molecular chaperone by interacting with unfolding proteins to prevent their aggregation and precipitation. (
  • The enhanced molecular chaperone activity of immobilized αB-crystallin has potential applications in preventing protein misfolding, including against amyloid disease processes, such as dialysis-related amyloidosis, and for biodiagnostic detection of misfolded proteins. (
  • Downregulated RV proteins included contractile elements: troponin T and C (-1.6 fold change), myosin regulatory light chain 2 (-1.9), cellular energetics modifier: fatty-acid binding protein (-1.5), and (3) ROS scavenger: superoxide dismutase 1 (-1.7). (
  • In the RA, 22 proteins spots were altered including the following downregulated proteins contractile elements: tropomyosin 1 alpha chain (-1.9), cellular energetic proteins: ATP synthase (-1.5), fatty-acid binding protein (-2.5), and (3) polyubiquitin (-3.5). (
  • Protection by αΒ-crystallin overexpression is linked to maintenance of proper mitochondrial protein levels, inhibition of abnormal mitochondrial permeability transition pore activation and maintenance of mitochondrial membrane potential (Δψ m ). (
  • Mouse anti Human alpha B crystallin antibody recognizes alpha B crystallin, also known as heat shock protein beta-5, heat-shock 20 kD like-protein and renal carcinoma antigen NY-REN-27, rosenthal fiber component. (
  • This orchestrated set of changes included proteins involved in cytoskeletal reorganization (caldesmon, calponin, alpha B-crystallin, stathmin, collapsing response mediator protein-2), cell adhesion (vinculin, galectin-1), signal transduction (RACK-1) and astrocyte differentiation (glutamine synthetase). (
  • Two protein bands of 19-20 kDa were identified as A-crystallins on 1-D immunoblots and these bands separated into 10 discrete spots on 2-D immunoblot. (
  • ABSTRACT alphaB- crystallin is a member of the sHSP (Small heat shock protein ) family , which plays an impor tant role in multiple neurodegeneration diseases . (
  • 5. The method for identification of the morbidity of epithelial ovarian cancer based on a tissue-type according to claim 3, wherein the protein E(+) specifically showing an upregulation in expression only in Endometrioid Carcinoma type is selected from the group consisting ofASRGL1 protein, andParvalbumin alpha. (
  • Nuclear staining for the small heat shock protein alphaB-crystallin colocalizes with splicing factor SC35. (
  • A method for expressing proteins as a fusion chimera with a domain of p26 or alpha crystallin type proteins to improve the protein stability and solubility when over expressed in bacteria such as E. coli is provided. (
  • Genes of interest are cloned into the multiple cloning site of the Vector System just downstream of the p26 or alpha crystallin type protein and a thrombin cleavage site. (
  • 2. The device of claim 1 , wherein the alpha-A-crystallin protein is a bovine alpha-A-crystallin protein. (
  • 3. The device of claim 1 , wherein the alpha-A-crystallin protein has an ability to inhibit protease. (
  • 8. The device of claim 1 , wherein the resin material coupled to the alpha-A-crystallin protein is cyanogen bromide activated sepharose. (
  • 12. A method of preparing device according to claim 1 , comprising the steps of a) mixing an alpha-A-crystallin protein with a buffer to form a coupling buffer mixture, and b) contacting an agarose resin with the coupling buffer mixture to form a prepared filter resin. (
  • 16. The method of claim 12 , wherein the alpha-A-crystalline protein is a bovine alpha-A-crystallin protein. (
  • 17. The method of claim 12 , wherein the alpha-A-crystalline protein has an ability to inhibit protease. (
  • They share characteristic homology with the $\alpha-crystallin$ protein of the mammalian eye lens as well as being ATP. (
  • Wang K, Spector A (2000) $\alpha$ -Crystalline prevents irreversible protein denaturation and acts cooperatively with other heat-shock proteins to renature and stabilize partially denatured protein in an ATP-dependent manner. (
  • de Jong WW, Leunissen JAM, Voorter CEM (1993) Evolution of the $\alpha$ -crystalline/small heat shock protein family. (
  • Four mutations are located in the Hsp20-alpha-crystallin domain, and one mutation is in the C-terminal part of the HSP27 protein. (
  • Defects in CRYAB are the cause of myofibrillar alpha-B crystallin-related (MFM-CRYAB) [MIM:608810]. (
  • To examine if decreased αB-crystallin levels were important for the manifestation of the behavioral phenotypes in the AD model mice we crossed Tg2576 mice (B6/SJL background) with wild-type (CRYAB+/+HSPB2+/+) or αB-crystallin/HspB2 knockout (CRYAB-/-HSPB2-/-) mice (129Sv background). (
  • In this hospital-based study, 1232 patients with breast cancer and an equal number of healthy controls in central Taiwan were genotyped via polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) and the association of CRYAB A-1215G (rs2228387), C-802G (rs14133) and intron 2 (rs2070894) polymorphisms with breast cancer risk in a central Taiwanese population was investigated. (
  • The coding regions of the CRYAA and CRYAB gene were amplified using polymerase chain reaction and subjected to restriction digestion. (
  • Alpha crystallins have features of molecular chaperones, but do not renature and release proteins like true chaperones. (
  • Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products--alpha-A and alpha-B. There are at least 5 different proteins comprising the beta-crystallins. (
  • Human endometrium expresses a molecular repertoire which includes the heat shock proteins (Hsps) Hsp27, Hsp60, Hsp70, Hsp90, and alpha crystallin B chain. (
  • Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. (
  • Mouse eye extracts and recombinant proteins (Crystallin-alpha A and B) were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-Crystallin alpha A (1:1000). (
  • To determine comparative effects of ultraviolet (UV)-A irradiation on structural and functional properties of wild type (WT) alphaB-crystallin and its three deamidated mutant proteins (alphaB-Asn78Asp, alphaB-Asn146Asp, and alphaB-Asn78/146Asp). (
  • The WT alphaB-crystallin and its three deamidated mutant proteins showed enhanced dimerization to 40 kDa species and partial degradation with increasing doses during UV-A-exposure. (
  • Similarly, UV-A-exposure altered the Trp microenvironment in the deamidated mutant proteins compared to the WT alphaB-crystallin. (
  • Hsp90s such as 1am1 , Hsp100s and small heat shock proteins like (alpha)-crystallin proteins. (
  • 1 α-, β-, and γ-Crystallins constitute the three classes of lens crystallins and account for 90% of total lens proteins. (
  • 2 αA-crystallin and αB-crystallin, the subunits of the oligomeric α-crystallin, are expressed in abundance in the lens and represent approximately 35% of the total lens proteins. (
  • α-Crystallin functions as a molecular chaperone by suppressing the unfolding of proteins 3 4 5 6 7 8 or by mediating in the refolding of unfolded proteins. (
  • Because the lens crystallins are the longest-lived proteins of the body with little turnover, they are an ideal system for analyzing posttranslational modifications associated with aging and evaluating the effect of these modifications on the protein's tertiary structure and subsequent cross-linking, aggregation, and insolubilization. (
  • ABSTRACT αA- and αB-crystallins are small heat shock proteins that bind thermodynamically destabilized proteins thereby inhibiting their aggregation. (
  • Highly expressed in the mammalian lens, the α-crystallins have been postulated to play a critical role in the maintenance of lens optical properties by sequestering age-damaged proteins prone to aggregation as well as through a multitude of roles in lens epithelial cells. (
  • 16 As a molecular chaperone, α-crystallin binds to misfolded proteins and suppresses their nonspecific aggregation. (
  • HSPB8 is part of the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. (
  • Immobilized αB-crystallin exhibited a significant enhancement (up to 5000-fold, when compared with the equivalent activity of αB-crystallin in solution) of its chaperone activity against various proteins undergoing both amorphous and amyloid fibril forms of aggregation. (
  • Crystallins are a diverse group of proteins that constitute nearly 90% of the total soluble proteins of the vertebrate eye lens and these tightly packed crystallins are responsible for transparency of the lens. (
  • At week two, which is marked by lens fiber differentiation genes encoding 12 crystallins and 3 lens-specific structural proteins were severely down-regulated. (
  • Although crystallins are highly conserved proteins among vertebrates, a significant number of polymorphisms exist in human population. (
  • Immunocytochemistry/ Immunofluorescence: AlphaA Crystallin/CRYAA Antibody (1H3.B8) [NBP2-12875] - Tissue: Neuroblastoma cell line SK-N-BE. (
  • A (p.R21Q), in the Crystallin alpha A ( CRYAA ) gene segregating in this three generation family. (
  • The work strengthens the genotype-phenotype correlations between this functional mutation in the crystallin alpha A ( CRYAA ) gene and paediatric cataract. (
  • alpha-A crystallin (CRYAA), beta-B2 crystallin (CRYBB2), gamma-A crystallin (CRYGA), gamma-B crystallin (CRYGB), gamma-C crystallin (CRYGC), gamma-D crystallin (CRYGD), gap junction alpha-3 (GJA3), gap junction alpha-8 (GJA8), and PAX6 based on polymerase chain reaction and single strand conformation polymorphism (PCR-SSCP) analysis. (
  • 0.5 ug/ml was sufficient for detection of 100 ng purified alphaA crystalline by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.Alpha A Crystallin Antibody.Use in Immunoblotting reported in scientific literature (PMID 28546921). (
  • Western blot analysis of whole cell lysates probed with alpha B crystallin antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P ) and visualized on the ChemiDoc MP with 4 second exposure. (
  • Mouse anti Human alpha B crystallin chain antibody detects a band of 22 kDa. (
  • A-crystallins, member of the -crystallin family, which are molecular chaperons and play crucial role in maintaining lens transparency were identified by 1-and 2-D immunoblot analysis with anti-A-crystallin antibody. (
  • Since there is only a single copy of the alphaB-crystallin gene, the two classes of mRNAs are generated by alternative transcriptional initiation from different promoters and their expressions are regulated differentially. (
  • Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. (
  • The aim of this study was to elucidate the molecular mechanisms that lead to a dominant nuclear cataract in a mouse harboring the Y118D mutation in the αA-crystallin gene. (
  • One such model is the Nuc1 rat model that arose from a spontaneous mutation in the Cryba1 (crystallin, beta 1) gene and exhibits complete failure of the hyaloid vasculature to regress. (
  • Gene expression patterns was examined by microarray analysis and quantitative polymerase chain reaction (QPCR). (
  • Also these results suggest that crystallin gene expression can be regulated by pro-inflammatory events in the eye. (
  • Mutation screening and genotype phenotype correlation of alpha-crystallin, gamma-crystallin and GJA8 gene in congenital cataract. (
  • Individual lens crystallins in water-soluble fractions were separated by gel-permeation chromatography. (
  • Lens crystallins play a crucial role in maintaining the clarity of the lens and its refractive properties. (
  • Profiles of lens crystallins were analyzed and crystallin proteome maps of Rita rita were generated for the first time. (
  • Results of the present study can be the baseline information for stimulating further investigation on Rita rita lens crystallins for comparative lens proteomics. (
  • Ageing and vision: structure, stability and function of lens crystallins. (
  • 1 2 Although the molecular weight of each individual subunit is approximately 20 kDa, the α-crystallins exist as multimers, with mass ranging from ∼300 to more than 1000 kDa. (
  • Among the four spots recovered after two-dimensional (2D)-gel electrophoresis from WT and the three deamidated species, the Met and Trp residues of alphaB-Asn146Asp mutant showed maximum oxidation after UV-A exposure, which might account for its greater loss in chaperone activity compared to WT alphaB-crystallin and two other deamidated species. (
  • Loss of αA-crystallin chaperone activity seems to be related to truncation of the C-terminal amino acid residues. (
  • This study clearly demonstrated that both the length and electrostatic charge of the COOH-terminal segment play crucial roles in governing the structural stability and chaperone activity of alphaB-crystallin . (
  • Changes in the self-association of alpha-crystallin aggregate were correlated to changes in chaperone activity. (
  • Alpha-crystallin from young donors, and recombinant alpha A- and alpha B-crystallin and their mixtures showed chaperone activity, which was markedly lost in samples from the aged alpha-crystallin aggregates. (
  • Structural perturbation (e.g., partial unfolding) enhances the in vitro chaperone activity of αB-crystallin. (
  • αB-crystallin was immobilized onto a range of solid material surfaces, and its characteristics and chaperone activity were assessed. (
  • Yan H, Yao L, Hui Y. [Decreased chaperone activity of alpha-crystallin by carbamylation in vitro]. (
  • B) Immunoblots showing αB-crystallin and Hsp27 expression in two sets of mice at 7 months of age. (
  • The chaperone expression in each genotype was confirmed by western-blotting two sets of brain lysates from 7-month old mice for αB-crystallin, and Hsp27 (Figure 2B). (
  • A hallmark of the microarray analysis is that the antagonist seems to be a novel stage-specific regulator of crystallin genes. (
  • Genes associated with the development of lens include crystallin genes. (
  • Purpose: Identification of causal mutation in the crystallin, connexin, and paired box 6 (PAX6) genes associated with childhood cataract in patients from India. (
  • In its native state, α-crystallin is water soluble and exists as an oligomer with a molecular mass of 360 to 800 kDa. (
  • Various classes of molecular chaperones cooperate for the folding of the nascent polypeptide chains in the cyotplasm [1] . (
  • the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. (
  • These heterogeneous aggregates consist of 30-40 subunits of the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. (
  • Lens alpha-crystallin, composed of two subunits alpha A- and alpha B-crystallin, forms large aggregates in the lens of the eye. (
  • One of the alpha crystallin subunits. (
  • The physicochemical properties of α-crystallin obtained from mouse lenses with the Y118D mutation as well as a recombinant Y118D αA-crystallin were studied using gel filtration, two-dimensional (2D) gel electrophoresis, multi-angle light scattering, circular dichroism, fluorescence, and chaperone activities. (
  • The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identified in bovine and rat lens. (
  • beta and gamma crystallins are also considered as a superfamily. (
  • Gamma-crystallins are involved in cataract formation. (
  • Pathogenesis of axonal dystrophy and demyelination in alphaA-crystallin-expressing transgenic mice. (
  • To investigate the influence of diabetes on the cleavage of C-terminal amino acid residues of αA- and αB-crystallins in human and rat lenses. (
  • 9 10 αA-crystallin and αB-crystallin, each with a molecular mass of 20 kDa, share 54% sequence homology 11 12 and are composed of 173 and 175 amino acid residues, respectively. (
  • Immobilization was achieved via a plasma-deposited thin polymeric interlayer containing aldehyde surface groups and reductive amination, leading to the covalent binding of αB-crystallin lysine residues to the surface aldehyde groups via Schiff-base linkages. (
  • These light chains are nearly identical except for first 41 additional amino acid residues of the A1 light chain, containing repeated Ala-Pro sequence and two pairs of lysine residues located near the N -terminus [ 5 ]. (
  • Crystallins are the principal structural components of the vertebrate eye lens. (
  • Conclusions IF and TFEB activation are clinically relevant therapeutic strategies to rescue advanced R120G αB-crystallin mutant-induced cardiomyopathy by normalizing desmin localization via autophagy-dependent and autophagy-independent mechanisms. (
  • The co- localization between alphaB- crystallin and abnormal deposition of PrPsc in the brain tissues of the scrapie infected hamsters was not observed. (
  • UV-A-induced structural and functional changes in human lens deamidated alphaB-crystallin. (
  • Species-specific structural and functional divergence of α-crystallins: Zebrafish αBa- and rodent αA(ins)-crystallin encode activated chaperones. (
  • Interestingly, αB-crystallin/HspB2 deficient transgenic mice were severely impaired in locomotion compared to each genetic model separately. (
  • Based on our observations of reduced αB-crystallin in older transgenic but not in non-transgenic mice, we hypothesized that if this chaperone is protective, then its loss may show an exaggerated phenotype. (
  • These abnormalities were less frequent in transgenic zebrafish embryos expressing rat αA-crystallin suggesting a specific role of α-crystallins in embryonic lens development. (
  • The penetrance of the lens phenotype was reduced by transgenic expression of rat αA-crystallin and its severity was attenuated by maternal αA-crystallin expression. (
  • However, anti-B-crystallin and antiphospho-B-crystallin antibodies were not able to detect any immunoreactive bands on 1- and 2-D immunoblots, indicating B-crystallin was either absent or present in extremely low concentration in Rita rita lens. (
  • Publications] Iwaki,T.: 'Preferential expression of αB-crystallin in astrocytic elements of neuroectodermal tumors. (
  • In the current study the authors investigated the expression of αB‐crystallin in four cultured glioma cell lines and in 115 human neuroectodermal tumors. (
  • To extend and confirm these findings, we generated an αA-crystallin knockout zebrafish line. (
  • αB‐crystallin was expressed differentially by those glioma cell lines, but not by neuroblastoma cell lines. (
  • The genomic DNAs from frozen brain tissues were amplified by polymerase chain reaction and sequenced. (
  • Together, these studies strongly suggest that during human cataract formation, covalent changes occur in the C-terminal region of the alpha A2 molecule. (
  • Anti-human Crystallin alpha A mAb, clone c9F2, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant Crystallin alpha A. (
  • Even when recombinant alpha-crystallins were mixed in molar ratios equivalent to those found in vivo, similar S values as the native aged alpha-crystallin aggregates were not obtained. (
  • The alpha-crystallin can be modified in vitro by carbamylation through a high-molecular-weight aggregates formation. (
  • To address this hypothesis, we investigated the consequences of αΒ-crystallin overexpression in the desmin-deficient ( Des −/− ) mouse model, which possesses a combination of the pathologies found in most cardiomyopathies, with mitochondrial defects as a hallmark. (
  • Furthermore, we found that both desmin and αΒ-crystallin are localized at sarcoplasmic reticulum (SR)-mitochondria-associated membranes (MAMs), where they interact with VDAC, Mic60 - the core component of mitochondrial contact site and cristae organizing system (MICOS) complex - and ATP synthase, suggesting that these associations could be crucial in mitoprotection at different levels. (
  • Mitochondrial abnormalities seem to be a common feature of diseases in which desmin and/or αB-crystallin deficiencies are implicated. (
  • The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. (
  • Molecular analysis of alphaB-crystallin in central nervous system and in pathologic conditions. (
  • A number of human brains displaying a variety of pathologic changes were examine by immunohistochemistry with an anti-alphaB-crystallin antiserum and increased immunoreactivity was found in astrocytes and oligodendrocytes without the formation of RFs. (
  • Furthermore, some neurons in neurodegenerative disorders were also immunolabeled with anti-alphaB-crystallin antiserum. (
  • We also determined the structures of alphaB-crystallin mRNAs and the genomic structure. (
  • Two major classes of mRNAs for the alphaB-crystallin, about 0.9 and 1.2 kilobases in length, are expressed in rat brain and they differ in the lengths of their 5' leader sequences. (
  • Next, we examined primary structures of alphaB-crystallin in two cases of pathologically confirmed Alexander's disease. (
  • Three deamidated mutants previously generated from recombinant WT alphaB-crystallin, using a site-specific mutagenesis procedure as previously described [32], were used. (
  • The WT alphaB-crystallin and its three deamidated species were exposed to UV-A light (320-400 nm) at intensities of 20 or 50 J/cm(2). (
  • To give insight into the possible alternation and the role of aB- crystallin in prion disease , the alphaB- crystallin levels in the brain tissues of agent 263K-infected hamsters were evaluated. (
  • Western blots and IHC revealed that at the terminal stages of the diseases , the levels of alphaB- crystallin were increased up to 3-fold in the brain tissues of scrapie infected 263K hamsters compared with normal controls. (
  • Immunofluorescent assays revealed that the up-regulated alphaB- crystallin was mainly observed in astrocytes , but not in neurons . (
  • The study may provide a foundation for further revealing the potential role of alphaB- crystallin in prion disease . (
  • Immunofluorescence staining of phospho-alpha-B crystallin (Ser19) in U373 MG cells results in cytoplasmic staining. (
  • We compared thermally induced denaturation and aggregation of two isoforms of the isolated myosin head (myosin subfragment 1, S1) containing different "essential" (or "alkali") light chains, A1 or A2. (
  • alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. (
  • However, the dominant nuclear cataract is associated with a significant decrease in the amount of αA-crystallin, leading to a reduction in total chaperone capacity needed for maintaining lens transparency. (
  • Immobilized αB-crystallin was characterized by X-ray photoelectron spectroscopy, atomic force microscopy, and quartz crystal microgravimetry, which showed that ∼300 ng cm -2 (dry mass) of oligomeric αB-crystallin was bound to the surface. (
  • Crystallins are also found vertebrate skeletal muscle tissue. (
  • S1 prepared by chymotryptic digestion of skeletal muscle myosin lacks the regulatory light chain but does contain the essential light chain, also called alkali light chain [ 4 ]. (
  • In fast skeletal muscle myosin there are two kinds of alkali light chains, A1 and A2. (
  • A more consistent and severe lens phenotype was evident in maternal/zygotic αA-crystallin mutants compared to those observed by morpholino knockdown. (