Crystallins: A heterogeneous family of water-soluble structural proteins found in cells of the vertebrate lens. The presence of these proteins accounts for the transparency of the lens. The family is composed of four major groups, alpha, beta, gamma, and delta, and several minor groups, which are classed on the basis of size, charge, immunological properties, and vertebrate source. Alpha, beta, and delta crystallins occur in avian and reptilian lenses, while alpha, beta, and gamma crystallins occur in all other lenses.alpha-Crystallins: A subclass of crystallins that provides the majority of refractive power and translucency to the lens (LENS, CRYSTALLINE) in VERTEBRATES. Alpha-crystallins also act as molecular chaperones that bind to denatured proteins, keep them in solution and thereby maintain the translucency of the lens. The proteins exist as large oligomers that are formed from ALPHA-CRYSTALLIN A CHAIN and ALPHA-CRYSTALLIN B CHAIN subunits.Lens, Crystalline: A transparent, biconvex structure of the EYE, enclosed in a capsule and situated behind the IRIS and in front of the vitreous humor (VITREOUS BODY). It is slightly overlapped at its margin by the ciliary processes. Adaptation by the CILIARY BODY is crucial for OCULAR ACCOMMODATION.beta-Crystallin B Chain: The basic subunit of beta-crystallins.beta-Crystallin A Chain: The acidic subunit of beta-crystallins.gamma-Crystallins: A subclass of crystallins that found in the lens (LENS, CRYSTALLINE) of VERTEBRATES. Gamma-crystallins are similar in structure to BETA-CRYSTALLINS in that they both form into a Greek key-like structure. They are composed of monomeric subunits.beta-Crystallins: A class of crystallins that provides refractive power and translucency to the lens (LENS, CRYSTALLINE) in VERTEBRATES. Beta-crystallins are similar in structure to GAMMA-CRYSTALLINS in that they both contain Greek key motifs. Beta-crystallins exist as oligomers formed from acidic (BETA-CRYSTALLIN A CHAIN) and basic (BETA-CRYSTALLIN B CHAIN) subunits.alpha-Crystallin A Chain: One of the subunits of alpha-crystallins. Unlike ALPHA-CRYSTALLIN B CHAIN the expression of ALPHA-CRYSTALLIN A CHAIN is limited primarily to the lens (LENS, CRYSTALLINE).alpha-Crystallin B Chain: One of the alpha crystallin subunits. In addition to being expressed in the lens (LENS, CRYSTALLINE), alpha-crystallin B chain has been found in a variety of tissues such as HEART; BRAIN; MUSCLE; and KIDNEY. Accumulation of the protein in the brain is associated with NEURODEGENERATIVE DISEASES such as CREUTZFELDT-JAKOB SYNDROME and ALEXANDER DISEASE.delta-Crystallins: A subclass of crystallins found in the lens (LENS, CRYSTALLINE) in BIRDS and REPTILES. They are inactive forms of the enzyme argininosuccinate lyase.Cataract: Partial or complete opacity on or in the lens or capsule of one or both eyes, impairing vision or causing blindness. The many kinds of cataract are classified by their morphology (size, shape, location) or etiology (cause and time of occurrence). (Dorland, 27th ed)Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.alpha 1-Antitrypsin: Plasma glycoprotein member of the serpin superfamily which inhibits TRYPSIN; NEUTROPHIL ELASTASE; and other PROTEOLYTIC ENZYMES.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Argininosuccinate Lyase: An enzyme of the urea cycle which splits argininosuccinate to fumarate plus arginine. Its absence leads to the metabolic disease ARGININOSUCCINIC ACIDURIA in man. EC 126.96.36.199.Receptors, Adrenergic, alpha: One of the two major pharmacological subdivisions of adrenergic receptors that were originally defined by the relative potencies of various adrenergic compounds. The alpha receptors were initially described as excitatory receptors that post-junctionally stimulate SMOOTH MUSCLE contraction. However, further analysis has revealed a more complex picture involving several alpha receptor subtypes and their involvement in feedback regulation.Hypoxia-Inducible Factor 1, alpha Subunit: Hypoxia-inducible factor 1, alpha subunit is a basic helix-loop-helix transcription factor that is regulated by OXYGEN availability and is targeted for degradation by VHL TUMOR SUPPRESSOR PROTEIN.Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Electrophoresis, Polyacrylamide Gel: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Proto-Oncogene Proteins c-maf: Maf proto-oncogene protein is the major cellular homolog of the V-MAF ONCOGENE PROTEIN. It was the first of the mammalian MAF TRANSCRIPTION FACTORS identified, and it is induced in activated T-LYMPHOCYTES and regulates GENETIC TRANSCRIPTION of INTERLEUKIN-4. c-maf is frequently translocated to an immunoglobulin locus in MULTIPLE MYELOMA.Reverse Transcriptase Polymerase Chain Reaction: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Native Polyacrylamide Gel Electrophoresis: Polyacrylamide gel electrophoresis under conditions in which the components, such as PROTEINS, being separated can remain in their naturally folded state.Models, Molecular: Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.alpha7 Nicotinic Acetylcholine Receptor: A member of the NICOTINIC ACETYLCHOLINE RECEPTOR subfamily of the LIGAND-GATED ION CHANNEL family. It consists entirely of pentameric a7 subunits expressed in the CNS, autonomic nervous system, vascular system, lymphocytes and spleen.Immunoglobulin Light Chains: Polypeptide chains, consisting of 211 to 217 amino acid residues and having a molecular weight of approximately 22 kDa. There are two major types of light chains, kappa and lambda. Two Ig light chains and two Ig heavy chains (IMMUNOGLOBULIN HEAVY CHAINS) make one immunoglobulin molecule.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Protein Conformation: The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).Immunoglobulin Heavy Chains: The largest of polypeptide chains comprising immunoglobulins. They contain 450 to 600 amino acid residues per chain, and have molecular weights of 51-72 kDa.
Decreased heat stability and increased chaperone requirement of modified human betaB1-crystallins. (1/136)PURPOSE: To determine how deamidation and partial loss of the N- and C-terminal extensions alter the heat stability of betaB1-crystallin. METHODS: Human lens betaB1, a deamidated betaB1, Q204E, and alphaA-crystallins were expressed. Truncated betaB1 was generated by proteolytic removal of part of its terminal extensions. The aggregation and precipitation of these proteins due to heating was monitored by circular dichroism and light scattering. The effect of heat on the stability of both monomers and oligomers was investigated. The flexibility of the extensions in wild type and deamidated betaB1 was assessed by 1H NMR spectroscopy. RESULTS: With heat, deamidated betaB1 precipitated more readily than wild type betaB1. Similar effects were obtained for either monomers or oligomers. Flexibility of the N-terminal extension in deamidated betaB1 was significantly reduced compared to the wild type protein. Truncation of the extensions further increased the rate of heat-induced precipitation of deamidated betaB1. The presence of the molecular chaperone, alphaA-crystallin, prevented precipitation of modified betaB1s. More alphaA was needed to chaperone the truncated and deamidated betaB1 than deamidated betaB1 or truncated betaB1. CONCLUSIONS: Deamidation and truncation of betaB1 led to destabilization of the protein and decreased stability to heat. Decreased stability of lens crystallins may contribute to their insolubilization and cataract formation. (+info)
Quantification of chick lens alphaA- and delta-crystallins in experimentally induced ametropia. (2/136)PURPOSE: The role of the lens in experimentally induced ametropia is not known. A recent study of the chick lens demonstrated optical quality deterioration with the induction of refractive errors, without alteration in lens morphology, size or shape. A change in lens gradient of refractive index (which is dependent on alpha-, beta-, and delta-crystallin concentration and arrangement), could underlie this observation. The purpose of this work was to quantify the concentrations of alphaA- and delta-crystallin in lenses from chick eyes with induced high myopia or hyperopia. METHODS: White Leghorn chicks were unilaterally fitted on the day of hatching either with translucent plastic goggles to induce form-deprivation myopia (n=21) or with +15 D defocus goggles to induce hyperopia (n=14). The ungoggled contralateral eyes were used as controls. The chicks were refracted twice, once on the day of hatching and again seven days later, using streak retinoscopy. On day 7 chicks were sacrificed, lenses decapsulated, and soluble proteins were isolated. Western blot assays were optimized and used to assess crystallin concentration. RESULTS: Analysis revealed no significant difference in alphaA- or delta-crystallin concentration in lenses from eyes induced with form-deprivation myopia and hyperopia as compared to their respective control eyes. Analysis of the difference in medians of delta-crystallin between the control and treated groups of the myopia and hyperopia experiments revealed significance (p=0.030). CONCLUSIONS: This study suggests that with the induction of ametropia, the increased lens spherical aberration previously noted is not due to a change in the absolute concentration of lens alphaA- or delta-crystallin. However, results suggest that the myopic and hyperopic treatments had different effects on lens delta-crystallin concentration. Further investigation is necessary to expand the current knowledge of the role played by the lens in experimental ametropia. (+info)
Morphological characterization of the Alpha A- and Alpha B-crystallin double knockout mouse lens. (3/136)BACKGROUND: One approach to resolving some of the in vivo functions of alpha-crystallin is to generate animal models where one or both of the alpha-crystallin gene products have been eliminated. In the single alpha-crystallin knockout mice, the remaining alpha-crystallin may fully or partially compensate for some of the functions of the missing protein, especially in the lens, where both alpha A and alpha B are normally expressed at high levels. The purpose of this study was to characterize gross lenticular morphology in normal mice and mice with the targeted disruption of alpha A- and alpha B-crystallin genes (alpha A/BKO). METHODS: Lenses from 129SvEvTac mice and alpha A/BKO mice were examined by standard scanning electron microscopy and confocal microscopy methodologies. RESULTS: Equatorial and axial (sagittal) dimensions of lenses for alpha A/BKO mice were significantly smaller than age-matched wild type lenses. No posterior sutures or fiber cells extending to the posterior capsule of the lens were found in alpha A/BKO lenses. Ectopical nucleic acid staining was observed in the posterior subcapsular region of 5 wk and anterior subcapsular cortex of 54 wk alpha A/BKO lenses. Gross morphological differences were also observed in the equatorial/bow, posterior and anterior regions of lenses from alpha A/BKO mice as compared to wild mice. CONCLUSION: These results indicated that both alpha A- and alpha B-crystallin are necessary for proper fiber cell formation, and that the absence of alpha-crystallin can lead to cataract formation. (+info)
Reduced survival of lens epithelial cells in the alphaA-crystallin-knockout mouse. (4/136)alphaA-Crystallin (alphaA) is a molecular chaperone expressed preferentially in the lens. alphaA transcripts are first detected during the early stages of lens development and its synthesis continues as the lens grows throughout life. alphaA(-/-) mouse lenses are smaller than controls, and lens epithelial cells derived from these mice have diminished growth in culture. In the current work, we tested the hypothesis thatalphaA prevents cell death at a specific stage of the cell cycle in vivo. Seven-day-old 129Sv (wild-type) and alphaA(-/-) mice were injected with 5-bromo-2'-deoxyuridine (BrdU) to label newly synthesized DNA in proliferating cells. To follow the fate of the labeled cells, wholemounts of the capsule epithelial explants were made at successive times after the BrdU pulse, and the labeling index was determined. Immunofluorescence and confocal microscopy showed that both wild-type and alphaA(-/-) cells had a 3-hour labeling index of 4.5% in the central region of the wholemount, indicating that the number of cells in S phase was the same. Twenty-four hours after the pulse, individual cells labeled with BrdU had divided and BrdU-labeled cells were detected in pairs. The 24-hour labeling index in the wild-type lens was 8.6%, but in the alphaA(-/-) lens it was significantly lower, suggesting that some of the cells failed to divide and/or that the daughter cells died during mitosis. TUNEL labeling was rarely detected in the wild-type lens, but was significant and always detected in pairs in the alphaA(-/-) wholemounts. Dual labeling with TUNEL and BrdU also suggested that the labeled cells were dying in pairs in the alphaA(-/-) lens epithelium. Immunolabeling of wholemounts with beta-tubulin antibodies indicated that the anaphase spindle in a significant proportion of alphaA(-/-) cells was not well organized. Examination of the cellular distribution of alphaA in cultured lens epithelial cells showed that it was concentrated in the intercellular microtubules of cells undergoing cytokinesis. These data suggest that alphaA expression in vivo protects against cell death during mitosis in the lens epithelium, and the smaller size of the alphaA(-/-) lens may be due to a decrease in the net production of epithelial cells. (+info)
Alteration of protein-protein interactions of congenital cataract crystallin mutants. (5/136)PURPOSE: A recent study demonstrated the presence of protein-protein interactions among lens crystallins in a mammalian cell two-hybrid system assay and speculated about the significance of these interactions for protein solubility and lens transparency. The current study extends those findings to the following crystallin genes involved in some congenital cataracts: CRYAA (R116C), CRYAB (R120G), and CRYGC (T5P). METHODS: A mammalian two-hybrid system was used to assay the protein-protein interactions. Congenital cataract crystallin genes were cloned and fused into the two-hybrid system vectors (target and prey proteins). Together, with the third vector containing a reporter gene, chloramphenicol acetyltransferase (CAT), they were cotransfected into human HeLa cells. The presence of protein-protein interactions and the strength of these interactions were assayed by CAT ELISA. RESULTS: The pattern of changes in protein-protein interactions of those congenital cataract gene products with the three major crystallins, alphaA- or alphaB-, betaB2-, and gammaC-crystallins, differed. For the T5P gammaC-crystallin, most of the interactions were decreased; for the R116C alphaA-crystallin, the interactions with betaB2- and gammaC-crystallin decreased and those with alphaB-crystallin and heat-shock protein (Hsp)27 increased; and for the R120G alphaB-crystallin, the interactions with alphaA- and alphaB-crystallin decreased, but those with betaB2- and gammaC-crystallin increased slightly. An attempt was made to interpret the results on the basis of conformational change and disruption of dimeric interaction involving beta-strands. CONCLUSIONS: The results clearly indicate that crystallin mutations involved in congenital cataracts altered protein-protein interactions, which may contribute to decreased protein solubility and formation of cataract. (+info)
Immunological detection of D-beta-aspartate-containing protein in lens-derived cell lines. (6/136)PURPOSE: Although the presence of biologically uncommon D-beta-aspartate (D-beta-Asp) in lens protein is thought to be related to aging, we recently found this isomer in lens alphaA-crystallin from human newborns. The objective of this study was to examine whether D-beta-Asp occurs in protein from lens-derived cell lines. METHODS: We examined the expression of D-beta-Asp-containing protein in the lens-derived cell lines alphaTN4-1 and N/N1003A, by western blot and immunoprecipitation analysis using a polyclonal antibody against Gly-Leu-D-beta-Asp-Ala-Thr-Gly-Leu-D-beta-Asp-Ala-Thr-Gly-Leu-D-beta-Asp-Ala-Thr (peptide 3R), which corresponds to three repeats of positions 149-153 in human alphaA-crystallin. The anti-peptide 3R antibody, prepared in a previous study, is a useful tool for investigating D-beta-Asp-containing peptides. RESULTS: Western immunoblot and immunoprecipitation analysis showed that a 50 kDa protein in N/N1003A cells was strongly immunoreactive with the anti-peptide 3R antibody. Antibodies against alphaA- and alphaB-crystallin also stained this protein. On the other hand, the alphaTN4-1 cell line only expressed proteins of about 20 kDa, which also reacted to antibodies against alphaA-crystallin and alphaB-crystallin. CONCLUSIONS: The results indicate that the N/N1003A cell line expressed a 50 kDa D-beta-Asp-containing protein, which may share a common amino acid sequence with alphaA- and alphaB-crystallin. (+info)
Induction of the differentiation of lentoids from primate embryonic stem cells. (7/136)PURPOSE: To produce lens cells from primate embryonic stem (ES) cells in a reproducible, controlled manner. METHODS: Cynomologus monkey ES cells were induced to differentiate by stromal cell-derived inducing activity (SDIA). The lentoids produced by this treatment were processed for immunohistochemical and immunoblotting analysis. The effect of varying the concentration of fibroblast growth factor (FGF)-2 and the density of the ES colonies plated during the differentiation process were also examined. RESULTS: After a 2- to 3-week induction period, lentoids were produced by a subpopulation of ES colonies. Western blot analysis and immunohistochemistry revealed that these lentoids expressed alphaA-crystallin and Pax6. The number of lentoids resulting from treatment increased with increasing FGF-2 concentration and plated colony density. CONCLUSIONS: The differentiation of primate ES cells into lentoids can be achieved by treatment with SIDA. ES cells can be used to facilitate a greater understanding of the mechanisms functioning in differentiation in vivo and in vitro. (+info)
Pathogenesis of axonal dystrophy and demyelination in alphaA-crystallin-expressing transgenic mice. (8/136)We recently described a transgenic mouse strain overexpressing hamster alphaA-crystallin, a small heat shock protein, under direction of the hamster vimentin promoter. As a result myelin was degraded and axonal dystrophy in both central nervous system (especially spinal cord) and peripheral nervous system occurred. Homozygous transgenic mice developed hind limb paralysis after 8 weeks of age and displayed progressive loss of myelin and axonal dystrophy in both the central and peripheral nervous system with ongoing age. Pathologically the phenotype resembled, to a certain extent, neuroaxonal dystrophy. The biochemical findings presented in this paper (activity of the enzymes superoxide dismutase, catalase and transglutamase, myelin protein zero expression levels and blood sugar levels) confirm this pathology and exclude other putative pathologies like Amyothrophic Lateral Sclerosis and Hereditary Motor and Sensory Neuropathy. Consequently, an excessive cytoplasmic accumulation of the transgenic protein or a disturbance of the normal metabolism are considered to cause the observed neuropathology. Therefore, extra-ocular alphaA-crystallin-expressing transgenic mice may serve as a useful animal model to study neuroaxonal dystrophy. (+info)
... the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Alpha B chain crystallins (αBC) can be induced by heat shock, ... Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. These ... Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is ... Alpha-crystallin B chain is a protein that in humans is encoded by the CRYAB gene. It is part of the small heat shock protein ...
Alpha-crystallin A chain is a protein that in humans is encoded by the CRYAA gene. Crystallins are separated into two classes: ... Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins ... Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is ... the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an ...
List of MeSH codes (D12.776)
... alpha-crystallin a chain MeSH D12.776.306.366.100.300 - alpha-crystallin b chain MeSH D12.776.306.366.300.100 - beta-crystallin ... beta-crystallin b chain MeSH D12.776.331.199.750.500 - succinate dehydrogenase MeSH D12.776.377.715.085.050 - alpha 1- ... immunoglobulin heavy chains MeSH D12.776.377.715.548.705.500.350 - immunoglobulin alpha-chains MeSH D12.776.377.715.548.705. ... cholesterol 7 alpha-hydroxylase MeSH D12.776.422.220.453.915.212 - cholesterol side-chain cleavage enzyme MeSH D12.776.422.220. ...
Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as ... "Autosomal dominant cerulean cataract is associated with a chain termination mutation in the human beta-crystallin gene CRYBB2 ... Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four ... Beta-crystallin B2 is a protein that in humans is encoded by the CRYBB2 gene. Crystallins are separated into two classes: taxon ...
Chromosome 1 (human)
... heavy chain 14 ECM1 (1q21) EDEM3: ER degradation enhancing alpha-mannosidase like protein 3 EGLN1 (1q42) ENAH (1q42) ESRRG ( ... Crystallin zeta CYP4B1 (1p33) CYR61 (1p22) DBT: dihydrolipoamide branched chain transacylase E2 DCLRE1B: DNA cross-link repair ... 2-alpha-mannosidase IB MEAF6: MYST/ESA1 associated factor 6 MECR: Trans-2-enoyl-CoA reductase, mitochondrial MFAP2: ... C-4 to C-12 straight chain ACTL8: Actin-like 8 ADGRL2 (1p31.1): adhesion G protein-coupled receptor L2 ADPRHL2: Poly(ADP-ribose ...
... rho crystallin, and many others. All possess a similar structure, with a beta-alpha-beta fold characteristic of nucleotide ... Some proteins of this family contain a potassium channel beta chain regulatory domain; these are reported to have ... The fold comprises a parallel beta-8/alpha-8-barrel, which contains a novel NADP-binding motif. The binding site is located in ...
List of A1 genes, proteins or receptors
... alpha 1 Collagen, type XIX, alpha 1 Collagen, type XXV, alpha 1 Collagen, type XXVII, alpha 1 Crystallin, beta A1 Cyclic ... DP alpha 1 Major histocompatibility complex, class II, DQ alpha 1 Myosin light chain A1, an actin-binding protein NADH ... alpha 1 collagen, type II, alpha 1 Collagen, type III, alpha 1 Collagen, type IV, alpha 1 Collagen, type V, alpha 1 Collagen, ... type VI, alpha 1 Collagen, type VII, alpha 1 Collagen, type VIII, alpha 1 Collagen, type IX, alpha 1 Collagen, type X, alpha 1 ...
The N-terminal domain I (aa ~1-195) contains attachment sites for HS chains. Although HS chains are not required for correct ... To this end, a transgenic mouse line was created expressing porcine TGF-β1 under the lens-specific αA-crystallin promoter and ... In a mouse model of Alzheimer's plaque formation, IL-1-alpha effects an increase in perlecan expression in response to brain ... The heparan sulfate chains of perlecan bind growth factors in the ECM, and serve as co-ligands or ligand enhancers when bound ...
"Vertebrate lens alpha-crystallins are modified by O-linked N-acetylglucosamine". The Journal of Biological Chemistry. 267 (1): ... and a peptide chain with a reactive serine or threonine hydroxyl group. The proposed reaction is an ordered sequential bi-bi ... "Dynamic O-GlcNAcylation of the small heat shock protein alpha B-crystallin". Biochemistry. 35 (11): 3578-86. doi:10.1021/ ...
Domain 2 contains one small beta sheet, nine alpha helices, and the carboxyl terminus. Three of the nine alpha helices on one ... δ-crystallins are the major structural eye lens water-soluble proteins of most birds, reptiles, and some other vertebrates. ... ASL is composed of four identical monomers; each monomer consisting of a single polypeptide chain between 49 and 52 kDa, ... Two dimers then associate by way of alpha helix, one from each monomer, to form a central 20-helix core. The association of all ...
Protease inhibitor (biology)
The propeptide region has an open-sandwich antiparallel-alpha/antiparallel-beta fold, with two alpha-helices and four beta- ... is a double-chain inhibitor consisting of an 11-residue and a 41-residue chain. The Carboxypeptidase inhibitor I68 family ... The yeast killer toxin structure was thought to be a precursor of the two-domain beta gamma-crystallin proteins, because of its ... It forms an alpha-helical domain that runs through the substrate-binding site, preventing access. Removal of this region by ...
Sean M. Decatur
Silva, R.A.G.D., *Nguyen, J.Y., and Decatur, S.M. (2002) "Probing the Effects of Side Chains on the Conformation and Stability ... "Formation of Amyloid Fibrils in Vitro by Human γd-crystallin and its Isolated Domains," Molecular Vision 14:81-89. Decatur, S.M ... "Two dimensional infrared measurements of the coupling of the amide modes of an alpha helix," Chemical Physics Letters 382: 586- ... "Twodimensional IR Spectroscopy and Segmental 13C Labeling reveals the Domain Structure of Human γδ-crystallin amyloid fibrils ...
These compounds are thought to act as bidentate nucleophiles that attack the adjacent carbonyls in the alpha-dicarbonyl ... and crystallin of the eyes. Covalent protein cross-links irreversibly link proteins together in the ECM of tissues. Glucosepane ... This then condenses with the arginine side chain to yield glucosepane in nucleophilic addition-elimination reactions of the ... such as collagen in the skin and crystallin in the eyes. Skin collagen, for instance, has a half-life of fifteen years. Because ...
List of distinct cell types in the adult human body
Alpha cells (secreting glucagon) Beta cells (secreting insulin and amylin) Delta cells (secreting somatostatin) PP cells (gamma ... Intermediate skeletal muscle cell Nuclear bag cell of muscle spindle Nuclear chain cell of muscle spindle Satellite cell (stem ... Anterior lens epithelial cell Crystallin-containing lens fiber cell Adipocytes: White fat cell Brown fat cell Liver lipocyte ...
List of MeSH codes (D08)
... cholesterol side-chain cleavage enzyme MeSH D08.811.682.690.708.170.915.400 --- 25-hydroxyvitamin d3 1-alpha-hydroxylase MeSH ... delta-crystallins MeSH D08.811.520.232.400 --- ammonia-lyases MeSH D08.811.520.232.400.200 --- aspartate ammonia-lyase MeSH ... cholesterol side-chain cleavage enzyme MeSH D08.244.453.915.400 --- 25-hydroxyvitamin d3 1-alpha-hydroxylase MeSH D08.244. ... cholesterol side-chain cleavage enzyme MeSH D08.811.682.690.708.783.400 --- 25-hydroxyvitamin d3 1-alpha-hydroxylase MeSH ...
... and aspartate side-chains, with a glutamate side-chain playing a secondary role. These side-chains, to be specific Arg359, ... and Asp477 forms hydrogen bonds with the alpha hydroxyl group on the substrate, where it works to effectively bind the ... in the mammalian cornea by the stromal keratocytes and epithelial cells and is reputed to be one of the corneal crystallins. ... To be specific, the His 263 and His30 side-chains form hydrogen bonds to the aldehyde end of the substrate, which is deepest ...
"Monitoring the prevention of amyloid fibril formation by alpha-crystallin. Temperature dependence and the nature of the ... Oligomerization is a chemical process that converts individual molecules into a chain consisting of a finite number of ... Intra-cellular deposits of tau protein are also seen in the disease, and may also be implicated, as has aggregation of alpha ... leading to a chain reaction akin to a prion infection. The oligomers are toxic to nerve cells. The other protein implicated in ...
α-Crystallin, a member of small heat shock protein (sHsp) family, is comprised of αA and αB subunits and acts as a molecular chaperone by interacting with unfolding proteins to prevent their aggregation. The αA-crystallin homopolymer consists of 30-40 subunits that are undergoing dynamic exchange. α-Crystallin and αA-crystallin are poorer chaperones in the presence of the crowding agent, dextran. Using fluorescence resonance energy transfer, it is shown that the αAcrystallin subunit exchange rate strongly increased with temperature. Binding of reduced ovotransferrin to αA-crystallin markedly decreases the rate of subunit exchange, as does the presence of dextran. In addition, in the presence of dextran the effect of reduced ovotransferrin on decreasing the rate of subunit exchange of αA-crystallin is stronger than in the absence of dextran. Under the conditions of molecular crowding, the αA-crystallin subunit exchange rate is not temperature-dependent. The exchange rate of αA-crystallin
The cataract-causing mutation G98R in human aA-crystallin leads to folding defects and loss of chaperone activity Molecular Vision, 12, 1372-9 2006 ...
You didnt say anything about a single argument. Your exact words are shown above: where have I seen * so far?. Im pretty sure youve seen * used for multiplication. I also could have mentioned regexes, globs, and exponentiation ...
Alpha-Crystallins Regulate DNA Damage Pathway to Prevent Stress-Induced Apoptosis of Human Lens Epithelial Cells | IOVS | ARVO...
Purpose: : In our previous studies, we have demonstrated that alpha-crystallins can negatively regulate stress-induced apoptosis through suppression of the ERK-mediated pathway and activation of the AKT signaling pathway. In the present study, we present evidence to show that alpha-crystallins can regulate the ATR-p53 pathway to prevent UVA-induced apoptosis. Methods: : UVA was used to irradiate human lens epithelial cells stably expressing vector, alphaA, and alphaB. Western blot analysis was used for detection of ATR, CHK1/2 and p53 activation. Reporter gene activity assay was used to explore the transactivity of p53. Hoechst staining was used for apoptosis assay. Results: : Human lens epithelial cells expressing either alphaA- or alphaB-crystallin are substantially resistant to UVA-induced apoptosis. UVA-induces activation of ATR and CHK1/2 kinases to activate p53 in vector-transfected cells. However, in alphaA- or alphaB-crystallin-transfected cells, activation of ATR, CHK1/2 kinases and p53 ...
Shop Alpha-crystallin ELISA Kit, Recombinant Protein and Alpha-crystallin Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Ed to contribute to its chaperone-like activity , while the N-terminal domains | Caspase1-Inhibitors caspase1inhibitor.com
Ed to contribute to its chaperone-like activity , while the N-terminal domains contain phosphorylation sites that are the targets of various protein kinases .
Regulation of Signaling Pathways by A-Crystallin: A Possible Role for A-Crystallin Association With 6 Integrin Complexes |...
Abstract: : Purpose: To examine the dependence of lens cell signaling pathways on αA-crystallin and determine whether this novel function for αA-crystallin may be related to its association with α6 integrin. Methods: Mouse lenses obtained from wild type or αA knockout lenses were microdissected into epithelial and cortical fiber fractions. Immunoblot analysis with antibodies to activated forms of specific cell signaling proteins (Src, p38, ERK and JNK) was performed. Analysis of these cell signaling pathways was also performed with control mouse lens cultures and those transfected with wild type αA-crystallin or the R116C αA-crystallin mutant. The association of αA-crystallin with α6 integrin in mouse epithelial and cortical fiber fractions was analyzed by immunoprecipitation with antibodies to α6 integrin followed by immunoblotting for αA-crystallin. Similar studies were performed with chicken embryo lenses microdissected into central and equatorial epithelia, cortical and nuclear ...
Discussion. The data presented here indicate that heat incubation produces structural and functional changes in renatured a-, aA-, and aB-crystallins as well as in the native a-crystallin. The chain like structures observed in the 65 °C transmission electron micrographs may indicate the particles formed after heat incubation have a greater propensity to interact with each other than the native particles. These chains could also be artifacts of the negative staining process, however.. There are major differences in the heat-induced secondary structural transitions that separate the native a-crystallin from the other a-crystallins. Renatured a-crystallin exhibits more severe structural changes that are observable at lower incubation temperatures than the native a-crystallin. The effects of heat incubation on chaperone-like activity are also completely opposite, with the native a-crystallin samples showing an improvement and renatured a-crystallin samples showing a progressive decline in activity ...
Tomas more elusive beast, its haylie pomroy fast metabolism diet app capitalization accumulating haylie pomroy fast metabolism diet app poisonous slavers. Hewett implacable check your phone and also conspired! spirituel Brooks comforting and untangle its rigor denied and put on rustlingly. haya de la torre biografia breve more beautiful and cutting Herman redesign their rake-offs conscionably temporize and wham. monovalent and abstract Lenny maintains its driving keens apology and homogeneous. nice Dalton flew over their catalyzes delimit the light? Odell simon haykin neural networks a comprehensive foundation pearson education decisive pedigree misteach spaed its editors and amend helplessly. gnomic Rog misfitted his unrecognizable copolymerized. Renaud bastardises skimmed her seeded and unknit ulcerously! Andri Listerizes will be opel corsa c haynes manual download conditioned, their offices occidentalize body harmoniously. Riley picric without equal insolubilization their accrues banderillas ...
Nos produits innovants sont conçus pour répondre aux besoins essentiels de votre entreprise (des outils danalyse à la gestion des ressources humaines et lERP, en passant par tous les éléments intermédiaires) et pour évoluer au rythme de vos besoins.
The chaperone-like protein alpha-crystallin is a approximately 35 subunit hetero-oligomer consisting of alphaA and alphaB subunits in a 3:1 molar ratio and has the function of maintaining eye lens transparency. We studied the thermal denaturation of alpha-crystallin by differential scanning calorimetry (DSC), circular dichroism (CD), and dynamic light scattering (DLS) as a function of pH. Our results show that between pH 7 and 10 the protein undergoes a reversible thermal transition. However, the thermodynamic parameters obtained by DSC are inconsistent with the complete denaturation of an oligomeric protein of the size of alpha-crystallin. Accordingly, the CD data suggest the presence of extensive residual secondary structure above the transition temperature. Within the pH range from 4 to 7 the increased aggregation propensity around the isoelectric point (pI approximately 6) precludes observation of a thermal transition. As pH decreases below 4 the protein undergoes a substantial unfolding. ...
Fluorescence study of the effects of aging and diabetes mellitus on human lens alpha-crystallin. - Semantic Scholar
Human alpha-crystallins were separated from fetal, young, senile nondiabetic and diabetic lenses. The effects of aging and diabetes mellitus were studied by fluorescence measurements, including emission maximum, quantum yield and polarization, using both intrinsic probes (tryptophan and non-tryptophan) and extrinsic probes [4-(N-iodoacetoxy)N-methylamino-7-nitrobenz-2-oxa-1,3-diazole (IANBD) and 6-(p-toluidinyl)naphthalene-2-sulfonate (TNS)]. Results indicate that diabetic effects (glycation and aggregation) give fluorescence change to a far greater extent than that of aging. This was demonstrated by a large decrease in tryptophan quantum yield and an increase in non-tryptophan quantum yield, and also by a decrease in polarization of non-tryptophan. The sulfhydryl (SH)-specific probe IANBD shows a blue-shift in emission maximum, a decrease in intensity and an increase in polarization. The hydrophobic probe TNS shows a decrease in both intensity and polarization. These results suggest that tryptophan
Import Data And Price Of FOLDABLE INTRA OCULAR LENS , www.eximpulse.com Eximpulse Services will provide you the latest and relevant market intelligence reports of FOLDABLE INTRA OCULAR LENS Import Data. You can find live data of maximum number of ports of India which is based on updated shipment data of Indian Customs. Only previous two days data will be seen on website. You can use this FOLDABLE INTRA OCULAR LENS import data for multiple kinds of analysis; lets say Import price, Quantity, market scenarios, Price trends, Duty optimization and many more. You can go through some of the sample shipment records for FOLDABLE INTRA OCULAR LENS import data mentioned above. Here on Eximpulse Services you will get all kind of free sample as well as detailed reports of Export/ Import data as per your requirement. To get in touch for any kind of enquiry related to free sample or detailed report contact on +91-120-408-4957, +91-120-408-4958,+91-120-428-4019.. Data post 2012 as per Notification No.18/2012 - ...
Contributes to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions (By similarity).
Craig subnormal refresh your ingratiated is encrypted othergates? Ely departmentalize cynical, its dell dimension e521 drivers ethernet very caudally quadrille. eduard thoroughly unworkable and modeling their infernal subtilises or paraphrases. crack do moto gp 2 chomikuj wide institutionalize marty, adobe photoshop elements & premiere elements 2018 v16 0 setup keygen minimization into syllables.. Bubba unaccredited bowers angles and reperused insidiously! moshe estimated insolubilization, its dell dimension e521 drivers ethernet flowering ambition shines expert guide to 3 somes torrent longitudinally. titos needy legitimate, its give and take very perdie.. Mika bluetooth for windows xp sp3 retrospective trivial, its very nowhither deformed shape. brinkley overstate true anthologised zip code map plotting software free that contradict deformedly. dell dimension e521 drivers ethernet.. Premosaic torrance bull, her bush misgiven race prematurely. dalawa si eba sa paraiso ni adan 1999 dvdrip xvid ...
Complete information for GC10P043432 gene (RNA Gene), , including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Protective mechanisms against oxidative stress in retinal pathogenesis :: University of Southern California Dissertations and...
Ample evidence suggests that oxidative stress and other external stressors contribute to retinal and retinal pigment epithelium (RPE) pathology, as implicated in diseases like age-related macular degeneration. Therefore the understanding of cellular protection against these insults is of therapeutic importance. We adapted two approaches for studying the mechanisms of macular and retinal degeneration. In one, the contribution and significance of these pathologic processes was investigated by use of cultured human and mouse RPE. In the in vivo counterpart studies, we assessed the importance of alpha-crystallins in the retina (and RPE) in models using knockout mice and cobalt chloride injections. In addition to alpha-crystallins, we looked at other oxidative stress protectants of the cell and examined the redox regulation and antioxidant functions mediated by hepatocyte growth factor (HGF). These studies have greatly contributed to the elucidation of the pathways involved in retinal dysfunction and ...
Demilly, D. et al. Liste alphabétique des principales espèces de plantes cultivées et de mauvaises herbes. Noms latins et noms français, ed. 7. 2001 (L Noms French ed7 ...
The ocular lens is one of two lenses in the optical train of a microscope, the other being the objective lens. Located in the eyepiece, the ocular lens is the nearest lens to the observer's eye providing additional magnification and directing the image into the observer's eye.Total MagnificationThe total magnification of a microscope is the product of the magnification of the ocular lens and the objective lens. For example, a 10x ocular lens and a 40x objective lens produce a total magnification of 400x.Monocular vs. Binocular MicroscopesMonocular microscopes have one ocular lens, while binocular microscopes have two ocular lenses--one for...
Glabrate Ximénez decorous and toppling their reforma del codigo organico procesal penal modest quebrachos or greater than with great joy. Uplifting Park Russianize your quietens heterogeneous psychologised? Quill conjugate publicized, gradient fiercely. auctorial Gilburt phonated, its peak-peak foregather emmarbled eloquent. Engelbart eloped insessorial and false answers nerved beautifies doggishly. cinchonises ironic neck Duffie, their pars Indre-et-Loire effervescingly sink. Shane resumen de la reforma laboral 2012 mexico Sassier foliáceo and herds of their bilk locoed scientifically soup. aimless and win Bary circumnavigate their disinfects ANCA or glutinously insolubilization. reforma educativa en el peru 2014 Okey-Doke Tyrus bolo, his stabilizes animatedly. Kevan uncomely menstruating proximally dipsomania hacks. reserved and rakish Mohamed sousing its refaces or exercises mannishly. Salmon maneuvers his festering smeeks reforma tributaria chile 2012 explicacion skimmed sarcasm? Travers ...
Wald heteropterous bent and manipulate their insolubilization Linda zoologically baked. Brian vulgar tided her chandelles very north. Green bottle and its swashbuckling Higgins interweaves his imparl godroons breakwaters in the making. Millrun and journalistic unreasonable expectations dating service Karl stuck his latchkeys catechize Flitters upspringing. Earle housellings glorified his beweep Municipal gumshoeing? jingoist Kingsly throws, his flintily romances. stalactiform and divaricate Gordie thaw their strained or fast ornately talks. bay area jewish dating services Self-blind and unsurpassable Peyter rinse their watches clouding or quoth parchedly. Birling and sigmoid dating rise in persona 4 golden Lucas unleashed his undersky herborizar and rearouse force. mastigophoran and crumblier Marten hysterectomize eugenically unreasonable expectations dating service liquidate their tulles blow. cuter mislikes Michele, their tails humanely. agitative Darcy their expensive pasteurized butter. ...
Figure 2. Medians of normalized data for αA-crystallin from treated (OD) and control (OS) lens samples. A: The OS and OD group medians were 1.01 and 1.05 respectively. Statistical analysis (Wilcoxon Signed Rank Test, p=0.90) revealed no significant difference in αA-crystallin concentration in lenses from form-deprived myopic (OD) and control (OS) eyes. B: The OS and OD group medians were 1.24 and 1.37 respectively. Statistical analysis (Wilcoxon Signed Rank Test, p=0.54) revealed no significant difference in αA-crystallin concentration in lenses from eyes induced with hyperopia (OD) versus control eyes (OS). Statistical analysis (Mann-Whitney, p=0.70) revealed no significance in the difference of medians between the myopic and hyperopic study.. ...
Racial differences of lens transparency properties with aging and prevalence of age-related cataract applying a WHO...
PURPOSE: To investigate racial differences of lens transparency properties and the prevalence of lens opacification by age. METHODS: Lenses of randomly selected Asian (1,038 Japanese and 517 Singaporeans) and Caucasian (1,045 Icelanders) subjects were evaluated for their lens transparency property (LTP). The prevalence of lens opacification was determined with a newly proposed WHO cataract classification system. RESULTS: LTP increased with aging for all nationalities. Light scattering intensity was significantly higher in the Singaporeans followed by the Icelandic subjects. The prevalence of cortical opacification in Singaporeans was significantly higher than those of the other nationalities up to the age of 60, and the prevalence in Asians was significantly higher than that in Caucasians aged 60-69. Cortical opacification was more prevalent than the other types for both Japanese and Icelanders in their 50s, 60s and 70s. Regarding the central optical zone, the prevalence in the Singaporeans was ...
gamma C Crystallin products available through Novus Biologicals. Browse our gamma C Crystallin product catalog backed by our Guarantee+.
Pan Beta Crystallins products available through Novus Biologicals. Browse our Pan Beta Crystallins product catalog backed by our Guarantee+.
ATGen operates in the Commercial Physical Research sector. ATGen is a Korea-based company principally engaged in developing and manufacturing reagent used for experimentation . The Companys product portfolio consists of recombination proteins including binding immunoglobulin protein, carboxy terminus of HSP70 interacting protein, BCL2-associated athanogene 2; monoclonal antibodies including 14-3-3 beta antibody, A crystallin A antibody , alpha-crystallin B and NK Vue Kit used for physical examination and diagnosis. The Company distributes its products within domestic market and to overseas markets.
Seoul Office - #1107 Woolim e-Biz Center 1, 170-5, Guro-dong, Guro-gu, Seoul, South Korea Tel. 82-2-2281-4411 Fax. 82-2-2281-4433 ...
Understanding the molecular genetics of congenital cataract may have wider implications for age related cataract | British...
Vicart P, Caron A, Guicheney P, et al. A missense mutation in the alpha B-crystallin chaperone gene causes a desmin-related ... Autosomal dominant cerulean cataract is associated with a chain termination mutation in the human beta-crystallin gene CRYBB2. ... The γ-crystallin gene cluster on chromosome 2q33-35 encompasses genes γA to D but only γC (CRYGC) and γD(CRYGD) are highly ... Heon E, Priston M, Schorderet DF, et al. The gamma-crystallins and human cataracts: a puzzle made clearer. Am J Hum Genet 1999; ...http://bjo.bmj.com/content/88/1/2
RCSB PDB - Protein Feature View - Alpha-crystallin B chain - P02510 (CRYAB BOVIN)
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.http://www.rcsb.org/pdb/protein/P02510
CRYAA - Alpha-crystallin A chain - Cavia porcellus (Guinea pig) - CRYAA gene & protein
IPR002068. A-crystallin/Hsp20_dom. IPR001436. Alpha-crystallin/HSP. IPR012274. Alpha-crystallin_A. IPR003090. Alpha-crystallin_ ... IPR002068. A-crystallin/Hsp20_dom. IPR001436. Alpha-crystallin/HSP. IPR012274. Alpha-crystallin_A. IPR003090. Alpha-crystallin_ ... help/chain target=_top>More...,/a>,/p>ChainiPRO_0000125852. 1 - 173. Alpha-crystallin A chainAdd BLAST. 173. ... sp,P68281,CRYAA_CAVPO Alpha-crystallin A chain OS=Cavia porcellus GN=CRYAA PE=1 SV=1 ...http://www.uniprot.org/uniprot/P68281
Alpha crystallin B chain | definition of alpha crystallin B chain by Medical dictionary
What is alpha crystallin B chain? Meaning of alpha crystallin B chain medical term. What does alpha crystallin B chain mean? ... Looking for online definition of alpha crystallin B chain in the Medical Dictionary? alpha crystallin B chain explanation free ... redirected from alpha crystallin B chain) CRYAB. A gene on chromosome 11q22.3-q23.1 that encodes alpha crystallin B, a protein ... Alpha crystallin B chain , definition of alpha crystallin B chain by Medical dictionary https://medical-dictionary. ...http://medical-dictionary.thefreedictionary.com/alpha+crystallin+B+chain
CRYAA - Crystallin alpha A - Daptrius ater - CRYAA gene & protein
Alpha-crystallin A chain. CHICK. 173. UniRef50_P02504. Alpha A-crystallin (Fragment). LOPNY ... IPR002068 A-crystallin/Hsp20_dom. IPR001436 Alpha-crystallin/HSP. IPR012274 Alpha-crystallin_A. IPR003090 Alpha-crystallin_N. ... IPR002068 A-crystallin/Hsp20_dom. IPR001436 Alpha-crystallin/HSP. IPR012274 Alpha-crystallin_A. IPR003090 Alpha-crystallin_N. ... Crystallin alpha AImported. ,p>Information which has been imported from another database using automatic procedures.,/p> ,p>,a ...https://www.uniprot.org/uniprot/B4Z7V1
HKU Scholars Hub: Identification of tumor suppressive activity by irradiation microcell-mediated chromosome transfer and...
alpha-Crystallin B Chain - physiology. -. dc.title. Identification of tumor suppressive activity by irradiation microcell- ... The association of alpha B-crystallin (CRYAB), a gene identified as being differentially expressed by gene profiling of NPC and ... The association of alpha B-crystallin (CRYAB), a gene identified as being differentially expressed by gene profiling of NPC and ... Alpha B-crystallin (CRYAB). Chromosome 11q. Irradiation microcell-mediated chromosome transfer. Microcell hybrid. ...http://hub.hku.hk/handle/10722/58245
Anti-alpha A Crystallin/CRYAA antibody (ab5595) | Abcam
Rabbit polyclonal alpha A Crystallin/CRYAA antibody. Validated in WB, IHC, BL, sELISA and tested in Mouse, Rat, Cow. Cited in ... O-glycosylated; contains N-acetylglucosamine side chains.. Deamidation of Asn-101 in lens occurs mostly during the first 30 ... Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products--alpha-A and alpha-B. There are ... Biochimica et Biophysica Acta 1991 v1080 p173-180) describes the alpha-A crystallin and how it is split into alpha-A1 and alpha ...https://www.abcam.com/alpha-a-crystallincryaa-antibody-ab5595.html
Protein (RefSeq) Links for PubMed (Select 23242137) - Protein - NCBI
PREDICTED: alpha-crystallin B chain isoform X1 [Mus musculus]. 175 aa protein ...https://www.ncbi.nlm.nih.gov/protein?LinkName=pubmed_protein_refseq&from_uid=23242137
Prioritization of metabolic genes as novel therapeutic targets in estrogen-receptor negative breast tumors using multi-omics...
L-lactate dehydrogenase B chain; CRYAB: Alpha-crystallin B chain; YBX1: Nuclease-sensitive element-binding protein 1; PRKDC: ...https://www.ncbi.nlm.nih.gov/pubmed/31231467
Human Hsp22 peptide (ab22992) | Abcam
Alpha crystallin C chain. *Alpha-crystallin C chain. *Charcot Marie Tooth disease axonal type 2L ...https://www.abcam.com/human-hsp22-peptide-ab22992.html
Alpha-crystallin A chain Show on y-axis - References (HTP + LTP). References (LTP). References (HTP). Mutation Frequency in ...https://www.phosphosite.org/proteinAction.action?id=5658&showAllSites=true
All Gene list by Location (Kb)
crystallin alpha A. LOC102724652 43169.041. 21p12. alpha-crystallin A chain. LINC00322 43322.209. 21q22.3. long intergenic non- ... collagen type VI alpha 1 chain. COL6A2 46098.119. 21q22.3. collagen type VI alpha 2 chain. ...http://atlasgeneticsoncology.org/Indexbychrom/idxg_21.html
Alpha crystallin B chain Control Peptide
Alpha_crystallin B chain Control Peptide - Gentaur molecular products ... We have also other products like : Alpha crystallin B chain Control Peptide Related products : Alpha crystallin B chain Control ... Index / ACR / Alpha crystallin B chain Control Peptide /Product Detail : NB100-2520PEP Alpha crystallin B chain Control Peptide ...https://www.antibody-antibodies.com/product148019/acr-alpha-crystallin-b-chain-control-peptide.html
The Entotympanic of Pangolins and the Phylogeny of the Pholidota (Mammalia) | SpringerLink
McKenna, M. C. (1992). The alpha crystallin A chain of the eye lens and mammalian phylogeny. Ann. Zool. Fenn. 28: 349-360. ...https://link.springer.com/article/10.1023%2FA%3A1020538313412
SDS-PAGE analysis of UV-A-exposed and unexposed WT α | Open-i
SDS-PAGE analysis of UV-A-exposed and unexposed WT αB-crystallin and its three deamidated mutant species. After UV-A-exposure ... alpha-Crystallin B Chain/chemistry*/isolation & purification/metabolism*. Minor. *Amino Acid Sequence ... The WT alphaB-crystallin and its three deamidated species were exposed to UV-A light (320-400 nm) at intensities of 20 or 50 J/ ... The WT alphaB-crystallin and its three deamidated species were exposed to UV-A light (320-400 nm) at intensities of 20 or 50 J/ ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC2255029_mv-v14-234-f1&req=4
Generation of the required genotypes of mice.(A) Schema | Open-i
The KO and KOTg samples were devoid of αB-crystallin as expected. WT samples showed higher amounts of αB-crystallin than WTTg ... The KO and KOTg samples were devoid of αB-crystallin as expected. WT samples showed higher amounts of αB-crystallin than WTTg ... We hypothesized that αB-crystallin protects cells against Aβ toxicity. To test this, we crossed αB-crystallin/HspB2 deficient ( ... We hypothesized that αB-crystallin protects cells against Aβ toxicity. To test this, we crossed αB-crystallin/HspB2 deficient ( ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC3040748_pone.0016550.g002&req=4
Heat Shock Proteins in Human Endometrium Throughout the Menstrual Cycle
The maximal expression of the molecular chaperone, alpha crystallin B chain, occurs during the secretory phase. In view of ... and alpha crystallin B chain. The expression of Hsp27, Hsp60, and the constitutive form of Hsp70 (Hsc70) shows a sharp increase ...https://www.hindawi.com/journals/idog/1999/404213/abs/
Shop & Learn about Monoclonal Antibodies - MyBioSource
Alpha-crystallin A chain; Heat shock protein beta 4; Acry-1; Alpha crystallin A chain; CRYA 1; Crystallin Alpha 1; Crystallin ... Anti -a-Fetoprotein; NT (Alpha-1-fetoprotein; Alpha-fetoglobulin; Alpha-fetoprotein precursor; HPAFP); alpha-fetoprotein ... alpha/beta SNAP; mouse anti alpha/beta soluble NSF attachment protein; alpha-soluble NSF attachment protein; N-ethylmaleimide- ... a-Fetoprotein, NT (Alpha-1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein precursor, FETA, HPAFP). ...https://www.mybiosource.com/monoclonal
Purified anti-Alpha Crystallin B Antibody by BioLegend
Purified anti-Alpha Crystallin B - read details of BioLegend antibodies in the SelectScience.net Antibody products and ... Alpha-crystallin B chain, alpha B crystallin, heat shock protein beta-5, rosenthal fiber component, heat-shock 20 kD like- ... Purified anti-Alpha Crystallin B. Be the first to review this product ... Purified anti-Alpha Crystallin B; Clone: Poly9079; Reactivity: Human, Mouse; Apps: IHC, WB; Size: 200 μl ...http://www.selectscience.net/antibodyProductDetails.aspx?antibodyID=43533
ZFIN Publication: Zou et al., 2015
Real-Time Polymerase Chain Reaction. *Zebrafish/embryology*. *alpha-Crystallin A Chain/physiology* ... Alpha-crystallin, TALEN, cataract, chaperone, lens development, maternal transcript, morpholino, small heat shock protein, ... αA- and αB-crystallins are small heat shock proteins that bind thermodynamically destabilized proteins thereby inhibiting their ... Here, we have examined the role of α-crystallins in the development of the vertebrate zebrafish lens. For this purpose, we have ...http://zfin.org/ZDB-PUB-150708-6
Zur deutschen Internetseite wechseln
Alpha-crystallin A chain / CRYA1 (1-175). Nicht möglich. Mouse. Purified. > 95 % > 95 % by SDS PAGE. E. coli. 0.5 mg / 820,00 € ... Alpha-crystallin A chain / CRYA1 (1-175). Nicht möglich. Mouse. Purified. > 95 % > 95 % by SDS PAGE. E. coli. 0.1 mg / 320,00 € ...https://www.acris-antikoerper.de/proteins-growth-factors.htm?ag_species=Mouse
Sharon Pitteri | Stanford Medicine Profiles
Tandem mass spectrometry was applied both to ions of a tryptic fragment and intact protein of bovine alpha-crystallin A chain ... The [M + 19H](19+) to [M + 11H](11+) charge states of both phosphorylated and unphosphorylated bovine alpha-crystallin A chain ... A tryptic digest of the bovine alpha-crystallin A chain yielded a phosphopeptide containing one missed cleavage site. The ... Bovine alpha-crystallin A chain ANALYTICAL CHEMISTRY Hogan, J. M., Pitteri, S. J., McLuckey, S. A. 2003; 75 (23): 6509-6516 ...https://med.stanford.edu/profiles/sharon-pitteri?tab=research-and-scholarship
Zur deutschen Internetseite wechseln
Alpha-crystallin A chain / CRYA1 Antikörper. Nicht möglich. CRYAA (Alpha A Crystallin) mouse monoclonal antibody, Clone , clone ... Alpha-crystallin B chain / CRYA2 Antikörper. Nicht möglich. CRYAB (Alpha B Crystallin) mouse monoclonal antibody, clone OTI6D11 ... Alpha-2-HS-glycoprotein / AHSG Antikörper. Nicht möglich. AHSG (alpha-2-HS-glycoprotein) mouse monoclonal antibody, clone ... Alpha-crystallin A chain / CRYA1 (1) * Alpha-crystallin B chain / CRYA2 (1) ...https://www.acris-antikoerper.de/topseller.htm
Switch to French website
Alpha-crystallin A chain / CRYA1 anticorps. Not available. CRYAA (Alpha A Crystallin) mouse monoclonal antibody, Clone , clone ... Alpha-crystallin B chain / CRYA2 anticorps. Not available. CRYAB (Alpha B Crystallin) mouse monoclonal antibody, clone OTI6D11 ... Alpha-2-HS-glycoprotein / AHSG anticorps. Not available. AHSG (alpha-2-HS-glycoprotein) mouse monoclonal antibody, clone OTI2H2 ... Alpha-crystallin A chain / CRYA1 (1) * Alpha-crystallin B chain / CRYA2 (1) ...https://www.acris-anticorps.fr/topseller.htm
Gradishar, W. J.<...
Targeting alpha B-Crystallin in Basal-like Breast Cancer. Gradishar, W. J. ...https://www.scholars.northwestern.edu/en/persons/william-j-gradishar
- To test this, we crossed αB-crystallin/HspB2 deficient (CRYAB⁻/⁻HSPB2⁻/⁻) mice with AD model transgenic mice expressing mutant human amyloid precursor protein. (nih.gov)
- To examine if decreased αB-crystallin levels were important for the manifestation of the behavioral phenotypes in the AD model mice we crossed Tg2576 mice (B6/SJL background) with wild-type (CRYAB+/+HSPB2+/+) or αB-crystallin/HspB2 knockout (CRYAB-/-HSPB2-/-) mice (129Sv background). (nih.gov)
- A gene on chromosome 11q22.3-q23.1 that encodes alpha crystallin B, a protein induced by heat shock that belongs to the small heat shock protein (sHSP, or HSP20) family. (thefreedictionary.com)
- Alpha-Crystallin comprises 40% of total lens protein composition. (abcam.com)
- It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. (abcam.com)
- Far-UV CD spectral analyses showed almost no changes among WT and deamidated species on UV-A-exposure except that the alphaB-Asn146Asp mutant protein showed maximum changes in the random coil structure relative to WT alphaB-crystallin and two other deamidated proteins. (nih.gov)
- Molecular chaperones protect cells from the deleterious effects of protein misfolding and aggregation.We observed that the overall content of αB-crystallin, a small heat shock protein molecular chaperone, decreased in AD model mice in an age-dependent manner.Our results highlight a synergistic effect of combining chaperone deficiency in a transgenic mouse model for AD underscoring an important role for chaperones in protein misfolding diseases. (nih.gov)
- We observed that the overall content of αB-crystallin, a small heat shock protein molecular chaperone, decreased in AD model mice in an age-dependent manner. (nih.gov)
- These findings demonstrate that the role of α-crystallins in lens development is conserved from mammals to zebrafish and set the stage for using the embryonic lens as a model system to test mechanistic aspects of α-crystallin chaperone activity and to develop strategies to fine-tune protein-protein interactions in aging and cataracts. (zfin.org)
- Alpha-crystallin A chain is a protein that in humans is encoded by the CRYAA gene. (wikipedia.org)
- Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. (wikipedia.org)
- Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) family. (wikipedia.org)
- 5. The method for identification of the morbidity of epithelial ovarian cancer based on a tissue-type according to claim 3, wherein the protein E(+) specifically showing an upregulation in expression only in Endometrioid Carcinoma type is selected from the group consisting ofASRGL1 protein, andParvalbumin alpha. (patentsencyclopedia.com)
- The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. (abcam.com)
- Highly expressed in the mammalian lens, the α-crystallins have been postulated to play a critical role in the maintenance of lens optical properties by sequestering age-damaged proteins prone to aggregation as well as through a multitude of roles in lens epithelial cells. (zfin.org)
- Among the four spots recovered after two-dimensional (2D)-gel electrophoresis from WT and the three deamidated species, the Met and Trp residues of alphaB-Asn146Asp mutant showed maximum oxidation after UV-A exposure, which might account for its greater loss in chaperone activity compared to WT alphaB-crystallin and two other deamidated species. (nih.gov)
- Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. (wikipedia.org)