A negative regulator of beta-catenin signaling which is mutant in ADENOMATOUS POLYPOSIS COLI and GARDNER SYNDROME.
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
A catenin that binds F-ACTIN and links the CYTOSKELETON with BETA CATENIN and GAMMA CATENIN.
A multi-functional catenin that participates in CELL ADHESION and nuclear signaling. Beta catenin binds CADHERINS and helps link their cytoplasmic tails to the ACTIN in the CYTOSKELETON via ALPHA CATENIN. It also serves as a transcriptional co-activator and downstream component of WNT PROTEIN-mediated SIGNAL TRANSDUCTION PATHWAYS.
A polyposis syndrome due to an autosomal dominant mutation of the APC genes (GENES, APC) on CHROMOSOME 5. The syndrome is characterized by the development of hundreds of ADENOMATOUS POLYPS in the COLON and RECTUM of affected individuals by early adulthood.
Tumor suppressor genes located in the 5q21 region on the long arm of human chromosome 5. The mutation of these genes is associated with familial adenomatous polyposis (ADENOMATOUS POLYPOSIS COLI) and GARDNER SYNDROME, as well as some sporadic colorectal cancers.
Major constituent of the cytoskeleton found in the cytoplasm of eukaryotic cells. They form a flexible framework for the cell, provide attachment points for organelles and formed bodies, and make communication between parts of the cell possible.
A family of cytoskeletal proteins that play essential roles in CELL ADHESION at ADHERENS JUNCTIONS by linking CADHERINS to the ACTIN FILAMENTS of the CYTOSKELETON.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Antibodies produced by a single clone of cells.
A multi-functional catenin that is highly homologous to BETA CATENIN. Gamma catenin binds CADHERINS and helps link their cytoplasmic tails to ACTIN in the CYTOSKELETON via ALPHA CATENIN. It is also found in DESMOSOMES where it mediates the link between DESMOSOMAL CADHERINS and DESMOPLAKIN.
A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.
Family of the suborder HAPLORHINI (Anthropoidea) comprising bipedal primate MAMMALS. It includes modern man (HOMO SAPIENS) and the great apes: gorillas (GORILLA GORILLA), chimpanzees (PAN PANISCUS and PAN TROGLODYTES), and orangutans (PONGO PYGMAEUS).
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Proteins that are involved in the peptide chain termination reaction (PEPTIDE CHAIN TERMINATION, TRANSLATIONAL) on RIBOSOMES. They include codon-specific class-I release factors, which recognize stop signals (TERMINATOR CODON) in the MESSENGER RNA; and codon-nonspecific class-II release factors.
A species of orangutan, family HOMINIDAE, found in the forests on the island of Sumatra.
A species of orangutan, family HOMINIDAE, found in the forests on the island of Borneo.
An island in the Malay Archipelago, east of Sumatra, north of Java, and west of Celebes. It is the third largest island in the world. Its name is a Portuguese alteration of BRUNEI, located on it. (From Webster's New Geographical Dictionary, 1988, p163; Room, Brewer's Dictionary of Names, 1992, p73)
A genus of orangutans in the family HOMINIDAE, comprising two species. Among the PRIMATES, the orangutan is second in size only to the gorilla (GORILLA GORILLA).
A republic stretching from the Indian Ocean east to New Guinea, comprising six main islands: Java, Sumatra, Bali, Kalimantan (the Indonesian portion of the island of Borneo), Sulawesi (formerly known as the Celebes) and Irian Jaya (the western part of New Guinea). Its capital is Djakarta. The ethnic groups living there are largely Chinese, Arab, Eurasian, Indian, and Pakistani; 85% of the peoples are of the Islamic faith.
Calcium-dependent cell adhesion proteins. They are important in the formation of ADHERENS JUNCTIONS between cells. Cadherins are classified by their distinct immunological and tissue specificities, either by letters (E- for epithelial, N- for neural, and P- for placental cadherins) or by numbers (cadherin-12 or N-cadherin 2 for brain-cadherin). Cadherins promote cell adhesion via a homophilic mechanism as in the construction of tissues and of the whole animal body.
Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.
Adherence of cells to surfaces or to other cells.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
The process by which cells convert mechanical stimuli into a chemical response. It can occur in both cells specialized for sensing mechanical cues such as MECHANORECEPTORS, and in parenchymal cells whose primary function is not mechanosensory.
Silicone polymers which consist of silicon atoms substituted with methyl groups and linked by oxygen atoms. They comprise a series of biocompatible materials used as liquids, gels or solids; as film for artificial membranes, gels for implants, and liquids for drug vehicles; and as antifoaming agents.
Synthetic organosiloxane gels that are formed from synthetic polymers of silicone oxide with organic sidechains (polydimethylsiloxane) by lengthening the polymer chains. Unlike silicone elastomers, they are not treated with amorphous silica. They are used as fillers in breast implants.
Desmoplakins are cytoskeletal linker proteins that anchor INTERMEDIATE FILAMENTS to the PLASMA MEMBRANE at DESMOSOMES.

The alphaE-catenin gene (CTNNA1) acts as an invasion-suppressor gene in human colon cancer cells. (1/431)

The acquisition of invasiveness is a crucial step in the malignant progression of cancer. In cancers of the colon and of other organs the E-cadherin/catenin complex, which is implicated in homotypic cell-cell adhesion as well as in signal transduction, serves as a powerful inhibitor of invasion. We show here that one allele of the alphaE-catenin (CTNNA1) gene is mutated in the human colon cancer cell family HCT-8, which is identical to HCT-15, DLD-1 and HRT-18. Genetic instability, due to mutations in the HMSH6 (also called GTBP) mismatch repair gene, results in the spontaneous occurrence of invasive variants, all carrying either a mutation or exon skipping in the second alphaE-catenin allele. The alphaE-catenin gene is therefore, an invasion-suppressor gene in accordance with the two-hit model of Knudsen for tumour-suppressor genes.  (+info)

Characterization of ZO-2 as a MAGUK family member associated with tight as well as adherens junctions with a binding affinity to occludin and alpha catenin. (2/431)

ZO-2, a member of the MAGUK family, was thought to be specific for tight junctions (TJs) in contrast to ZO-1, another MAGUK family member, which is localized at TJs and adherens junctions (AJs) in epithelial and nonepithelial cells, respectively. Mouse ZO-2 cDNA was isolated, and a specific polyclonal antibody was generated using corresponding synthetic peptides as antigens. Immunofluorescence microscopy with this polyclonal antibody revealed that, similarly to ZO-1, in addition to TJs in epithelial cells, ZO-2 was also concentrated at AJs in nonepithelial cells such as fibroblasts and cardiac muscle cells lacking TJs. When NH2-terminal dlg-like and COOH-terminal non-dlg-like domains of ZO-2 (N-ZO-2 and C-ZO-2, respectively) were separately introduced into cultured cells, N-ZO-2 was colocalized with endogenous ZO-1/ZO-2, i.e. at TJs in epithelial cells and at AJs in non-epithelial cells, whereas C-ZO-2 was distributed along actin filaments. Consistently, occludin as well as alpha catenin directly bound to N-ZO-2 as well as the NH2-terminal dlg-like portion of ZO-1 (N-ZO-1) in vitro. Furthermore, immunoprecipitation experiments revealed that the second PDZ domain of ZO-2 was directly associated with N-ZO-1. These findings indicated that ZO-2 forms a complex with ZO-1/occludin or ZO-1/alpha catenin to establish TJ or AJ domains, respectively.  (+info)

Cadherin-11 is expressed in invasive breast cancer cell lines. (3/431)

In several cancers, including breast cancer, loss of E-cadherin expression is correlated with a loss of the epithelial phenotype and with a gain of invasiveness. Cells that have lost E-cadherin expression are either poorly invasive with a rounded phenotype, or highly invasive, with a mesenchymal phenotype. Most cells lacking E-cadherin still retain weak calcium-dependent adhesion, indicating the presence of another cadherin family member. We have now examined the expression of the mesenchymal cadherin, cadherin-11, in breast cancer cell lines. Cadherin-11 mRNA and protein, as well as a variant form, are expressed in the most invasive cell lines but not in any of the noninvasive cell lines. Cadherin-11 is localized to a detergent-soluble pool and is associated with both alpha- and beta-catenin. Immunocytochemistry shows that cadherin-11 is localized to the cell membrane at sites of cell-cell contact as well as at lamellipodia-like projections, which do not interact with other cells. These results suggest that cadherin-11 expression may be well correlated with the invasive phenotype in cancer cells and may serve as a molecular marker for the more aggressive, invasive subset of tumors. Cadherin-11 may mediate the interaction between malignant tumor cells and other cell types that normally express cadherin-11, such as stromal cells or osteoblasts or perhaps even with the surrounding extracellular matrix, thus facilitating tumor cell invasion and metastasis.  (+info)

Functional domains of alpha-catenin required for the strong state of cadherin-based cell adhesion. (4/431)

The interaction of cadherin-catenin complex with the actin-based cytoskeleton through alpha-catenin is indispensable for cadherin-based cell adhesion activity. We reported previously that E-cadherin-alpha-catenin fusion molecules showed cell adhesion and cytoskeleton binding activities when expressed in nonepithelial L cells. Here, we constructed deletion mutants of E-cadherin-alpha-catenin fusion molecules lacking various domains of alpha-catenin and introduced them into L cells. Detailed analysis identified three distinct functional domains of alpha-catenin: a vinculin/alpha-actinin-binding domain, a ZO-1-binding domain, and an adhesion-modulation domain. Furthermore, cell dissociation assay revealed that the fusion molecules containing the ZO-1-binding domain in addition to the adhesion-modulation domain conferred the strong state of cell adhesion activity on transfectants, although those lacking the ZO-1-binding domain conferred only the weak state. The disorganization of actin-based cytoskeleton by cytochalasin D treatment shifted the cadherin-based cell adhesion from the strong to the weak state. In the epithelial cells, where alpha-catenin was not precisely colocalized with ZO-1, the ZO-1-binding domain did not completely support the strong state of cell adhesion activity. Our studies showed that the interaction of alpha-catenin with the actin-based cytoskeleton through the ZO-1-binding domain is required for the strong state of E-cadherin-based cell adhesion activity.  (+info)

Different behavior of l-afadin and neurabin-II during the formation and destruction of cell-cell adherens junction. (5/431)

We have recently isolated two novel actin filament-binding proteins, l-afadin and neurabin-II and shown that they are localized at cell-cell adherens junction (AJ) in epithelial cells. We found here that l-afadin, neurabin-II, ZO-1, and E-cadherin showed similar and different behavior during the formation and destruction of cell-cell AJ in MDCK cells. In MDCK cells, the accumulation of both l-afadin and E-cadherin, but not that of ZO-1, changed in parallel depending on Rac small G protein activity. Dissociation of MDCK cells by culturing the cells at 2 microM Ca2+ caused rapid endocytosis of E-cadherin, but not that of l-afadin or ZO-1. Addition of phorbol 12-myristate 13-acetate to these dissociated cells formed a tight junction-like structure where ZO-1 and l-afadin, but not neurabin-II or E-cadherin, accumulated. We furthermore found that, in non-epithelial EL cells, which expressed E-cadherin and attached to each other, l-afadin, neurabin-II, ZO-1 and E-cadherin were all localized at AJ. In cadherin-deficient L cells, I-afadin was mainly localized at cell-cell contact sites, but ZO-1 was mainly localized at the tip area of cell processes. Neurabin-II did not accumulate at the plasma membrane area. Neither l-afadin nor neurabin-II significantly interacted with alpha-, beta-catenin, E-cadherin, ZO-1 or occludin.  (+info)

Cell-extracellular matrix interactions and EGF are important regulators of the basal mammary epithelial cell phenotype. (6/431)

The mammary epithelium is composed of a luminal epithelium and a basal layer containing myoepithelial cells and undifferentiated precursors. Basal cells express specific protein markers, such as keratin 14 (K14) and P-cadherin. To study the factors that regulate the basal mammary epithelial cell phenotype, we have established two clonal derivatives of the mouse HC11 cell line, BC20 and BC44, expressing high levels of K14 and P-cadherin. Unlike the parental HC11 cells, these basal cells did not produce beta-casein in response to lactogenic hormone treatment; however their phenotype appeared to be plastic. Cultured in EGF-free medium, they exhibited enhanced cell-extracellular matrix adhesions and deficient cell-cell junctions, whereas long-term treatment with EGF induced a decrease of focal contact number and establishment of cell-cell junctions, resulting in downregulation of K14 and P-cadherin expression at the protein and mRNA levels. To determine whether cell-extracellular matrix interactions mediated by integrins have a role in the regulation of the expression of K14 and P-cadherin, the amounts of transcripts for the two proteins were analysed in the basal cells, which were plated on the function-blocking antibodies against beta1 and alpha6 integrin chains, on fibronectin and on laminin 5. The amount of P-cadherin transcript was 2- to 4-fold higher in cells plated on the function-blocking anti-integrin antibodies and on the extracellular matrix proteins, as compared to cells plated on poly-L-lysine, whereas the K14 transcript levels were not significantly modified in response to adhesion. The data demonstrate that integrin-mediated cell interaction with extracellular matrix is directly implicated in the control of P-cadherin expression, and that EGF and cell-extracellular matrix adhesion events are important regulators of the basal mammary epithelial cell phenotype.  (+info)

Rearrangement of adherens junctions by transforming growth factor-beta1: role of contraction. (7/431)

The signal transduction pathways that lead to disruption of pulmonary endothelial monolayer integrity by transforming growth factor-beta1 (TGF-beta1) have not been elucidated. The purpose of this investigation was to determine whether disassembly of the adherens junction is temporally associated with the TGF-beta1-induced decrease in pulmonary endothelial monolayer integrity. Measurement of albumin clearance and electrical resistance showed that monolayer integrity started to decrease between 1 and 2 h post-TGF-beta1 treatment and continued to slowly decrease over the next 6 h. Immunofluorescence microscopy of monolayers between 2 and 3 h post-TGF-beta1 showed that beta-catenin, plakoglobin, alpha-catenin, and cadherin-5 were colocalized both at the cell periphery and in newly formed bands that are perpendicular to the cell-cell border. At 4 h post-TGF-beta1, cells began separating; however, beta- and alpha-catenin, plakoglobin, and cadherin-5 could still be found at the cell periphery at areas of cell separation and in strands between separated cells. By 8 h, these junctional proteins were no longer present at the cell periphery at areas of cell separation. The myosin light chain kinase inhibitor KT-5926 prevented the TGF-beta1-induced change in integrity but did not inhibit the formation of actin stress fibers or the formation of bands containing adherens junction proteins that were perpendicular to the cell-cell junction. Overall, these results suggest that adherens junction disassembly occurs after cell separation during TGF-beta1-induced decreases in pulmonary endothelial monolayer integrity and that the loss of integrity may be due to the activation of a myosin light chain kinase-dependent signaling cascade.  (+info)

E-cadherin and alpha-, beta- and gamma-catenin expression in prostate cancers: correlation with tumour invasion. (8/431)

The E-cadherin-catenin complex plays an important role in establishing and maintaining intercellular connections and morphogenesis and reduced expression of its constituent molecules is associated with invasion and metastasis. In the present study, we examined E-cadherin and alpha-, beta- and gamma-catenin levels in tumour tissues obtained by radical prostatectomy in order to investigate the relationship with histopathological tumour invasion. Immunohistochemical findings for 45 prostate cancer specimens demonstrated aberrant expression of each molecule to be associated with dedifferentiation and, in addition, alteration of staining patterns for the three types of catenin was significantly correlated with capsular but not lymphatic or vascular invasion. The data thus suggest that three types of catenin may be useful predictive markers for biological aggressiveness of prostate cancer.  (+info)

US Biological Anti-Catenin, beta (Beta Catenin, Cadherin-associated Protein, Catenin beta 1, Catenin beta-1, CATNB, CTNNB, CTNNB1) SKU: C2069-51C()
Catenin Beta 1, CTNNB are cell adhesion molecules called (p120*␠-catenin) cadherins (the (CDH1) E-cadherin/catenin complex) include the beta-catenins a multifunctional molecule Locus: 3p22.1 [§§; ^]. Neurons also exhibited a higher CTNNB/TCF pathway association (concentration versus accumulation) with cadherins; CAS-chromosome segregation 1-like (yeast) binds with E-cadherin but not with beta-catenin. Which interacts with (Tcf-T-cell factor where a…
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Complete information for CTNNA1 gene (Protein Coding), Catenin Alpha 1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Human CTNNA2 qPCR primer pairs, confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
The cadherin-catenin complex is important for mediating homotypic, calcium-dependent cell-cell interactions in diverse tissue types. Although proteins of this complex have been identified, little is known about their interactions. Using a genetic assay in yeast and an in vitro protein-binding assay, we demonstrate that beta-catenin is the linker protein between E-cadherin and alpha-catenin and that E-cadherin does not bind directly to alpha-catenin. We show that a 25-amino acid sequence in the cytoplasmic domain of E-cadherin and the amino-terminal domain of alpha-catenin are independent binding sites for beta-catenin. In addition to beta-catenin and plakoglobin, another member of the armadillo family, p120 binds to E-cadherin. However, unlike beta-catenin, p120 does not bind alpha-catenin in vitro, although a complex of p120 and endogenous alpha-catenin could be immunoprecipitated from cell extracts. In vitro protein-binding assays using recombinant E-cadherin cytoplasmic domain and ...
In vitro studies with purified proteins showed that αE-catenin is an allosteric protein in which the monomer binds the cadherin-catenin complex and homodimer preferentially binds actin filaments and inhibits Arp2/3 complex-mediated actin polymerization (Drees et al., 2005; Yamada et al., 2005). These results intimated roles for αE-catenin in the regulation of actin-based membrane dynamics in addition to and perhaps independent of αE-catenin roles in cell-cell adhesion. In this study, we tested key predictions of these in vitro results in epithelial cells.. We identified multiple forms of αE-catenin in MDCK cells. A membrane-bound pool of αE-catenin was identified in the E-cadherin-catenin complex; formally, we do not know whether this pool is a monomer, homodimer, or both, although it is likely to be a monomer based upon structural evidence (Pokutta and Weis, 2000). In the cytosol, we identified αE-catenin monomer and homodimer, which had a relatively fast turnover rate. We also observed ...
In vitro studies with purified proteins showed that αE-catenin is an allosteric protein in which the monomer binds the cadherin-catenin complex and homodimer preferentially binds actin filaments and inhibits Arp2/3 complex-mediated actin polymerization (Drees et al., 2005; Yamada et al., 2005). These results intimated roles for αE-catenin in the regulation of actin-based membrane dynamics in addition to and perhaps independent of αE-catenin roles in cell-cell adhesion. In this study, we tested key predictions of these in vitro results in epithelial cells.. We identified multiple forms of αE-catenin in MDCK cells. A membrane-bound pool of αE-catenin was identified in the E-cadherin-catenin complex; formally, we do not know whether this pool is a monomer, homodimer, or both, although it is likely to be a monomer based upon structural evidence (Pokutta and Weis, 2000). In the cytosol, we identified αE-catenin monomer and homodimer, which had a relatively fast turnover rate. We also observed ...
A key component of the Wnt pathway, beta-catenin combines with alpha-catenin and regulates cell-cell adhesion. It also interacts with alpha-catenin in the nucleus.. The alpha-catenin component of this beta-catenin/alpha-catenin complex has an inhibitory effect on beta-catenin that helps keep tumor cell migration and invasion in check. This inhibition is lost, however, when the EGFR pathway is activated. Upon activation, beta-catenin becomes untethered from alpha-catenin and translocates to the cell nucleus, where it increases expression of key target genes involved in tumor cell invasion and metastasis.. New Pathway Regulates Beta-Catenin Transactivation. The M. D. Anderson-led team made a surprising discovery: Beta-catenin also can travel to the nucleus via activation of the EGFR pathway-and it does so independently of Wnt signaling or mutations. The newly described pathway disrupts the beta-catenin/alpha catenin complex through an EGFR-extracellular receptor kinase (ERK)-protein kinase CK2- ...
The increased penetrance, earlier onset, and increased severity of the cardiac phenotype in αMHC/Ecad mice suggests that the epithelial cadherin may further perturb the transmission of the contractile force across the intercalated disc structure. In contrast to chick N-cadherin, the less similar E-cadherin is more likely to interfere with adherens junction organization by intercalating into the cadherin zipper, thus disrupting the normal homogeneous clustering of N-cadherin/catenin complexes ( Fig. 9). Mouse and chicken N-cadherin are nearly identical in the cytoplasmic and transmembrane domains; therefore, we predict normal interaction(s) with the submembranous myofibril connection. In comparison, the cytoplasmic domain of E-cadherin is less similar (61%) and somewhat shorter (10 amino acids) than N-cadherin. Although TEM did not indicate any obvious defect in myofibril insertion at the cell membrane, it is possible that subtle molecular differences in the E-cadherin/myofibril connection may ...
Polyclonal antibody for ALPHA 1 CATENIN/CTNNA1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. ALPHA 1 CATENIN/CTNNA1 information: Molecular Weight: 100071 MW; Subcellular Localization: Isoform 1: Cytoplasm,
beta Catenin (phospho Thr41/Ser45) antibody (catenin (cadherin-associated protein), beta 1, 88kDa) for WB. Anti-beta Catenin (phospho Thr41/Ser45) pAb (GTX50180) is tested in Human samples. 100% Ab-Assurance.
beta Catenin antibody [1F3] (catenin (cadherin-associated protein), beta 1, 88kDa) for IHC-P, WB. Anti-beta Catenin mAb (GTX84650) is tested in Human, Monkey samples. 100% Ab-Assurance.
Increased transcriptional activity of beta-catenin resulting from Wnt/Wingless-dependent or -independent signaling has been detected in many types of human cancer, but the underlying mechanism of Wnt-independent regulation remains unclear. We demonstrate here that EGFR activation results in disruption of the complex of beta-catenin and alpha-catenin, thereby abrogating the inhibitory effect of alpha-catenin on beta-catenin transactivation via CK2alpha-dependent phosphorylation of alpha-catenin at S641. ERK2, which is activated by EGFR signaling, directly binds to CK2alpha via the ERK2 docking groove and phosphorylates CK2alpha primarily at T360/S362, subsequently enhancing CK2alpha activity toward alpha-catenin phosphorylation. In addition, levels of alpha-catenin S641 phosphorylation correlate with levels of ERK1/2 activity in human glioblastoma specimens and with grades of glioma malignancy. This EGFR-ERK-CK2-mediated phosphorylation of alpha-catenin promotes beta-catenin transactivation and tumor
Immunogen is synthetic peptide corresponding to the C-terminal region (amino acids 890-901) of human/mouse α-catenin conjugated to KLH. (ZFIN Staff ...
Sigma-Aldrich offers abstracts and full-text articles by [Xing-xing Chen, Jiang Lin, Jun Qian, Wei Qian, Jing Yang, Ji-chun Ma, Zhao-qun Deng, Cui An, Chun-yan Tang, Zhen Qian, Qing Liu].
Mouse Monoclonal Anti-CTNNA3 Antibody (892_24D2S) [Alexa Fluor® 405]. Validated: WB, Flow, ICC/IF, IHC, IHC-P. Tested Reactivity: Human. 100% Guaranteed.
Use Bio-Rads PrimePCR assays, controls, templates for your target gene. Every primer pair is optimized, experimentally validated, and performance guaranteed.
The pack contains capillary tubes of 3 µl (100 pieces). Order now lab equipment and supplies in Alpha-cat (international delivery)
TY - JOUR. T1 - The cadherin-catenin adhesion system in signaling and cancer. AU - Conacci-Sorrell, Maralice. AU - Zhurinsky, Jacob. AU - Ben-Zeev, Avri. PY - 2002/4/29. Y1 - 2002/4/29. UR - http://www.scopus.com/inward/record.url?scp=0036120562&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0036120562&partnerID=8YFLogxK. U2 - 10.1172/JCI200215429. DO - 10.1172/JCI200215429. M3 - Review article. C2 - 11956233. AN - SCOPUS:0036120562. VL - 109. SP - 987. EP - 991. JO - Journal of Clinical Investigation. JF - Journal of Clinical Investigation. SN - 0021-9738. IS - 8. ER - ...
Synapses are fundamental building blocks of neural circuits. Synapse formation requires complex regulation involving cell adhesion molecules, secreted molecules, transcription factors and so forth. For cell adhesion molecules, ...
Compare Alpha-catulin ELISA Kits from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more.
ENCODES a protein that exhibits actin filament binding (inferred); alpha-catenin binding (inferred); chromatin binding (inferred); INVOLVED IN positive regulation of kinase activity (ortholog); PARTICIPATES IN E-cadherin signaling pathway; ASSOCIATED WITH esophagus squamous cell carcinoma (ortholog); FOUND IN cytosol (inferred); focal adhesion (inferred); Golgi apparatus (inferred)
Modulating agents for inhibiting an interaction between &agr;-catenin and &bgr;-catenin are provided. The modulating agents comprise one or more of: (a) a &bgr;-catenin HAV motif; (b) a peptide analogue or mimetic of a &bgr;-catenin HAV motif; or (c) an antibody or antigen-binding fragment thereof that specifically binds to a &bgr;-catenin HAV motif. Methods for using such modulating agents for inhibiting cadherin-mediated cell adhesion in a variety of contexts are also provided.
The gene encoding α-T-catenin, CTNNA3,is positioned within a region on chromosome 10, showing strong evidence of linkage to Alzheimers disease (AD), and is therefore a good positional candidate...
ABCA4, ABCB6, ABCC6, ABHD12, ACBD5, ADAM9, ADAMTS18, ADGRV1, AGK, AHI1, AIPL1, ALMS1 (excluding exon 8), ARL13B, ARL6, ATF6, B3GLCT, BBS1, BBS10, BBS12, BBS2, BBS4, BBS5, BBS7, BBS9, BCOR, BEST1, BFSP2, BMP4, C19ORF12, C1QTNF5, C2orf71, C8ORF37, CA4, CABP4, CACNA1F, CACNA2D4, CC2D2A, CDH23, CDH3, CDHR1, CEP290, CEP41, CERKL, CFAP410 (C21orf2), CFH, CHM, CHMP4B, CHST6, CIB2, CLN3, CLN5, CLN6, CLN8, CLRN1, CNGA1, CNGA3, CNGB1, CNGB3, CNNM4, COL11A1, COL11A2, COL2A1, COL4A1, COL4A6, COL8A2, COL9A1, COL9A2, CRB1, CRX, CRYAA, CRYAB, CRYBA1, CRYBA4, CRYBB1, CRYBB2, CRYBB3, CRYGB, CRYGC, CRYGD, CRYGS, CTDP1, CTNNA1, CTSD, CYP1B1, CYP4V2, DCN, DHDDS, DSPP, EFEMP1, ELOVL4, EPHA2, EYS, FAM161A, FLVCR1, FRAS1, FREM1, FREM2, FSCN2, FTL, FYCO1, FZD4, GALK1, GALT, GDF3, GDF6, GFER, GIPC3, GJA1, GJA3, GNAT1, GNAT2, GNPTG, GPR143, GPR179, GRIP1, GRK1, GRM6, GRN, GSN, GUCA1A, GUCA1B, GUCY2D, HARS, HCCS, HMX1, HSF4, IDH3B, IFT140, IMPDH1, IMPG1, IMPG2, INVS, ITM2B, IQCB1, JAG1, JAM3, KARS1, KCNJ13, KCNV2, KIF11, ...
The cadherin-catenin complex (CCC) is crucial for successful completion of morphogenesis during embryonic development, and for suppression of metastatic phenoty...
TY - JOUR. T1 - Abnormal expression and function of the E-cadherin-catenin complex in gastric carcinoma cell lines. AU - Jawhari, A. U.. AU - Noda, M.. AU - Farthing, M. J.G.. AU - Pignatelli, M.. PY - 1999/1/1. Y1 - 1999/1/1. N2 - Dysfunction of the cadherin-catenin complex, a key component of adherens junctions, is thought to confer invasive potential to cells. The aim of this study is to examine the expression and function of the E-cadherin/catenin complex in gastric carcinoma cell lines. Expression of E-cadherin, α, β and γ-catenin and p120(ctn), and of the adenomatous polyposis coil protein (APC), together with function of the cadherin-catenin complex was examined in a panel of gastric carcinoma cell lines, using immunocytochemistry, Western blotting and a cell-cell aggregation assay. Protein interactions were examined by sequential immunoprecipitation and immunoblotting with antibodies to E-cadherin, α, β and γ-catenin, p120(ctn) and APC. Abnormalities of E-cadherin, α- and ...
Expression of CTNNA2 (CAP-R, CT114) in cancer tissue. The cancer tissue page shows antibody staining of the protein in 20 different cancers.
Background: Approximately one third of the hereditary diffuse gastric cancer (HDGC) families carry germline mutations in the E-cadherin gene (CDH1). Risk prediction in members of families with this rare but deadly cancer could be improved by the identification of additional HDGC genes in non-CDH1 families.. Methods: Affected individuals from 22 CDH1 mutation-negative families were screened for germline mutations in four catenin genes: CTNNA1, CTNNB1, JUP, and CTNND1. Catenins interact closely with E-cadherin molecules in cells, and are therefore logical candidate genes for mutation screening in HDGC families.. Results: No nonsynonymous variants were seen in CTNNA1, CTNNB1, or CTNND1; only JUP contained nonsynonymous variants, of which only two rare variants were predicted to be deleterious.. Conclusion: Catenin genes are not commonly mutated in non-CDH1 HDGC families.. Impact: Germline mutations in CTNNA1, CTNNB1, JUP, or CTNND1 are unlikely to play a major role in HDGC. Cancer Epidemiol ...
BACKGROUND: Approximately one third of the hereditary diffuse gastric cancer (HDGC) families carry germline mutations in the E-cadherin gene (CDH1). Risk prediction in members of families with this rare but deadly cancer could be improved by the identification of additional HDGC genes in non-CDH1 families.METHODS: Affected individuals from 22 CDH1 mutation-negative families were screened for germline mutations in four catenin genes: CTNNA1, CTNNB1, JUP, and CTNND1. Catenins interact closely with E-cadherin molecules in cells, and are therefore logical candidate genes for mutation screening in HDGC families.RESULTS: No nonsynonymous variants were seen in CTNNA1, CTNNB1, or CTNND1; only JUP contained nonsynonymous variants, of which only two rare variants were predicted to be deleterious.CONCLUSION: Catenin genes are not commonly mutated in non-CDH1 HDGC families.Impact: Germline mutations in CTNNA1, CTNNB1, JUP, or CTNND1 are unlikely to play a major role in HDGC. Cancer Epidemiol Biomarkers Prev; 1-3.
Cell-cell adherens junctions (AJs), the most common type of intercellular adhesions, are important for maintaining tissue architecture and cell polarity and can limit cell movement and proliferation. At AJs, E-cadherin serves as an essential cell adhesion molecules (CAMs). The cytoplasmic tail binds beta-catenin, which in turn binds alpha-catenin. Alpha-catenin is associated with F-actin bundles directly and indirectly. The integrity of the cadherin-catenin complex is negatively regulated by phosphorylation of beta-catenin by receptor tyrosine kinases (RTKs) and cytoplasmic tyrosine kinases (Fer, Fyn, Yes, and Src), which leads to dissociation of the cadherin-catenin complex. Integrity of this complex is positively regulated by beta -catenin phosphorylation by casein kinase II, and dephosphorylation by protein tyrosine phosphatases. Changes in the phosphorylation state of beta-catenin affect cell-cell adhesion, cell migration and the level of signaling beta-catenin. Wnt signaling acts as a ...
Cell-cell adherens junctions (AJs), the most common type of intercellular adhesions, are important for maintaining tissue architecture and cell polarity and can limit cell movement and proliferation. At AJs, E-cadherin serves as an essential cell adhesion molecules (CAMs). The cytoplasmic tail binds beta-catenin, which in turn binds alpha-catenin. Alpha-catenin is associated with F-actin bundles directly and indirectly. The integrity of the cadherin-catenin complex is negatively regulated by phosphorylation of beta-catenin by receptor tyrosine kinases (RTKs) and cytoplasmic tyrosine kinases (Fer, Fyn, Yes, and Src), which leads to dissociation of the cadherin-catenin complex. Integrity of this complex is positively regulated by beta -catenin phosphorylation by casein kinase II, and dephosphorylation by protein tyrosine phosphatases. Changes in the phosphorylation state of beta-catenin affect cell-cell adhesion, cell migration and the level of signaling beta-catenin. Wnt signaling acts as a ...
Cell-cell adherens junctions (AJs), the most common type of intercellular adhesions, are important for maintaining tissue architecture and cell polarity and can limit cell movement and proliferation. At AJs, E-cadherin serves as an essential cell adhesion molecules (CAMs). The cytoplasmic tail binds beta-catenin, which in turn binds alpha-catenin. Alpha-catenin is associated with F-actin bundles directly and indirectly. The integrity of the cadherin-catenin complex is negatively regulated by phosphorylation of beta-catenin by receptor tyrosine kinases (RTKs) and cytoplasmic tyrosine kinases (Fer, Fyn, Yes, and Src), which leads to dissociation of the cadherin-catenin complex. Integrity of this complex is positively regulated by beta -catenin phosphorylation by casein kinase II, and dephosphorylation by protein tyrosine phosphatases. Changes in the phosphorylation state of beta-catenin affect cell-cell adhesion, cell migration and the level of signaling beta-catenin. Wnt signaling acts as a ...
Cadherins are calcium-dependent, cell surface glycoproteins involved in cell-cell adhesion. To function in cell-cell adhesion, the transmembrane cadherin molecule must be associated with the cytoskeleton via cytoplasmic proteins known as catenins. Three catenins, alpha-catenin, beta-catenin, and gamma-catenin (also known as plakoglobin), have been identified. The domain of the cadherin molecule important for its interaction with the catenins has been mapped to the COOH-terminal 70 amino acids, but less is known about regions of the catenins that allow them to associate with one another or with the cadherin molecule. In this study we have transfected carboxyl-terminal deletions of plakoglobin into the human fibrosarcoma HT-1080 and used immunofluorescence localization and co-immunoprecipitation to map the regions of plakoglobin that allow it to associate with N-cadherin and with alpha-catenin. Plakoglobin is an armadillo family member containing 13 weakly similar internal repeats. These data show ...
Cadherins are calcium-dependent, cell surface glycoproteins involved in cell-cell adhesion. To function in cell-cell adhesion, the transmembrane cadherin molecule must be associated with the cytoskeleton via cytoplasmic proteins known as catenins. Three catenins, alpha-catenin, beta-catenin, and gamma-catenin (also known as plakoglobin), have been identified. The domain of the cadherin molecule important for its interaction with the catenins has been mapped to the COOH-terminal 70 amino acids, but less is known about regions of the catenins that allow them to associate with one another or with the cadherin molecule. In this study we have transfected carboxyl-terminal deletions of plakoglobin into the human fibrosarcoma HT-1080 and used immunofluorescence localization and co-immunoprecipitation to map the regions of plakoglobin that allow it to associate with N-cadherin and with alpha-catenin. Plakoglobin is an armadillo family member containing 13 weakly similar internal repeats. These data show ...
Cadherins are calcium-dependent, cell surface glycoproteins involved in cell-cell adhesion. To function in cell-cell adhesion, the transmembrane cadherin molecule must be associated with the cytoskeleton via cytoplasmic proteins known as catenins. Three catenins, alpha-catenin, beta-catenin, and gamma-catenin (also known as plakoglobin), have been identified. The domain of the cadherin molecule important for its interaction with the catenins has been mapped to the COOH-terminal 70 amino acids, but less is known about regions of the catenins that allow them to associate with one another or with the cadherin molecule. In this study we have transfected carboxyl-terminal deletions of plakoglobin into the human fibrosarcoma HT-1080 and used immunofluorescence localization and co-immunoprecipitation to map the regions of plakoglobin that allow it to associate with N-cadherin and with alpha-catenin. Plakoglobin is an armadillo family member containing 13 weakly similar internal repeats. These data show ...
We have investigated how cell contractility and adhesion are functionally integrated during epithelial morphogenesis. To this end, we have analysed the role of α-Catenin, a key molecule linking E-Cadherin-based adhesion and the actomyosin cytoskeleton, during Drosophila embryonic dorsal closure, by studying a newly developed allelic series. We find that α-Catenin regulates pulsatile apical contraction in the amnioserosa, the main force-generating tissue driving closure of the embryonic epidermis. α-Catenin controls actomyosin dynamics by stabilising and promoting the formation of actomyosin foci, and also stabilises DE-Cadherin (Drosophila E-Cadherin, also known as Shotgun) at the cell membrane, suggesting that medioapical actomyosin contractility regulates junction stability. Furthermore, we uncover a genetic interaction between α-Catenin and Vinculin, and a tension-dependent recruitment of Vinculin to amniosersoa apical cell membranes, suggesting the existence of a mechano-sensitive module ...
We show in this paper that this can indeed occur, in a cell culture system in which Wg signaling can be controlled and its response be measured over time. We found two contrasting effects: Wg initially lowers the amount of the cadherin-catenin complex at cell-cell contacts, most probably by reducing the Arm pool interacting with E-cadherin, but later, Wg signaling leads to elevated DE-cadherin transcription. This later increase in DE cadherin may titrate the pool of Arm available for Wg signaling and therefore attenuate the transcriptional response to Wg. We have shown by metabolic labeling experiments that the reduction in E-cadherin levels was caused by a posttranscriptional mechanism that affected the stability of the E-cadherin protein. Although levels of E-cadherin precursors were similar in the absence or presence of Wg signaling, very little mature E-cadherin was detectable in cells with an activated Wg pathway, indicating that the majority of E-cadherin was either degraded before arrival ...
BACKGROUND: In approximately 10% of all gastric cancer (GC) cases, a heritable cause is suspected. A subset of these cases have a causative germline CDH1 mutation; however, in most cases the cause remains unknown. Our objective was to assess to what extent these remaining cases may be explained by germline mutations in the novel candidate GC predisposing genes CTNNA1, MAP3K6 or MYD88. METHODS: We sequenced a large cohort of unexplained young and/or familial patients with GC (n=286) without a CDH1germline mutation for germline variants affecting CTNNA1, MAP3K6 and MYD88 using a targeted next-generation sequencing approach based on single-molecule molecular inversion probes ...
CTNNB1 - CTNNB1 (GFP-tagged) - Human catenin (cadherin-associated protein), beta 1, 88kDa (CTNNB1), transcript variant 2 available for purchase from OriGene - Your Gene Company.
TY - JOUR. T1 - β-Catenin serves as a clutch between low and high intercellular E-cadherin bond strengths. AU - Bajpai, Saumendra. AU - Feng, Yunfeng. AU - Wirtz, Denis. AU - Longmore, Gregory D.. PY - 2013/11/19. Y1 - 2013/11/19. N2 - A wide range of invasive pathological outcomes originate from the loss of epithelial phenotype and involve either loss of function or downregulation of transmembrane adhesive receptor complexes, including Ecadherin (Ecad) and binding partners β-catenin and α-catenin at adherens junctions. Cellular pathways regulating wild-type β-catenin level, or direct mutations in β-catenin that affect the turnover of the protein have been shown to contribute to cancer development, through induction of uncontrolled proliferation of transformed tumor cells, particularly in colon cancer. Using single-molecule force spectroscopy, we show that depletion of β-catenin or the prominent cancer-related S45 deletion mutation in β-catenin present in human colon cancers both weaken ...
When we examined solid pseudopapillary tumours of the pancreas, a tumour characterised by β‐catenin mutations within exon 3, these tumours showed a strong reduction in E‐cadherin:β‐catenin complexes. Moreover in HCC, a tumour type which has approximately 20% β‐catenin exon 3 mutations, there was a good correlation between reduction in E‐cadherin and activation of Wnt signalling targets. Thus, it may be that in these cancers, E‐cadherin limits the precise levels of Wnt signalling driven by β‐catenin mutation. Thus, downregulation of E‐cadherin in these tumours may drive tumour progression. It should also be noted that opposing patterns of Wnt signalling and E‐cadherin have been shown in murine liver, with β‐catenin higher in zone 3 of the liver and E‐cadherin in zone 1. Therefore, one might predict that β‐catenin mutations would yield a greater phenotype in hepatocytes from zone 3 of the liver versus zone 1 (Benhamouche et al, 2006). Hence, one could speculate ...
Cells in our body utilize a variety of adaptor proteins for transmitting context specific signals that arise from the cellular microenvironment. Adaptor proteins lack enzymatic activity and typically perform their function by acting as scaffolds that bind other signaling proteins. While most adaptor proteins are functionally modulated by biochemical alterations such as phosphorylation, a subset of adaptor proteins are functionally modulated by a mechanical alteration in their structure that makes cryptic sites available for binding to downstream signaling proteins. α-catenin is one such adaptor protein that localizes to cadherin-based cell adhesions by binding the membrane-localized cadherin-β-catenin complex at one side and the cytosolic F-actin on the other side. An increase in actomyosin tension is directly relayed to α-catenin resulting in a change in its conformation making cryptic binding sites accessible to its interacting partners. Here, I describe an updated view of the mechanical ...
My work examines the role of a conserved tryptophan residue, Trp2, in the adhesive domain of cadherins that has been shown to be essential for their adhesive function. Structural studies have shown Trp2 to be integrated into its own cadherin domain, integrated into the domain an opposing cadherin molecule, or freed from the domain and exposed to solvent. Until now, the physiological relevance of these structures has been controversial. Using conformation specific antibodies I show that Trp2 integrates into the domain fold of its own cadherin molecule in physiological conditions, but that this integration is not stable owing to structural constraints imposed by calcium binding to the cadherin. This raises the possibility that Trp2 could participate in intermolecular interactions during adhesion by inserting into opposing cadherins in what is referred to as the strand exchange model of cadherin adhesion. This model is tested directly by introducing cysteine substitutions into opposing cadherins ...
Cadherins ensure that cells within tissues are bound together. However, this is only one side of the coin. Dr. Almut Köhler from the Karlsruhe Institute of Technology (KIT) and her group of researchers are investigating a cadherin type that is even able to actively promote cell migration in developing frog brains and tumour tissue.
Regulating the cytosolic concentration of the protein β-catenin is an important cell proliferation control mechanism. The cytoplasmic concentration of β-catenin remains low through an interaction with a protein complex consisting of adenomatous polyposis coli (APC), Axin, protein phosphatase 2A, and glycogen synthase kinase 3β (GSK3β). Upon phosphorylation by GSK3β, β-catenin associates with a ubiquitin ligase (E3) complex, resulting in its ubiquitination and proteolysis. Activation of Wnt signaling inactivates GSK3, allowing β-catenin to accumulate in the cytoplasm and eventually to translocate to the nucleus so as to affect gene expression. Two groups report that a phosphorylation-independent mechanism can lead to the destruction of β-catenin. Matsuzawa and Reed show that a mammalian protein called Siah-1 lies at the hub of another pathway that destroys β-catenin in response to DNA damaging agents and the activation of the tumor suppressor protein p53. Siah-1 is known to interact with ...
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Our study unveils a TREM2/β-catenin pathway that regulates bone mass by regulating the rate of OC generation. Mechanistically, TREM2 and β-catenin augment the M-CSF-induced proliferation of OcP, retarding their differentiation into mature OC. Ablation of either TREM2 or β-catenin inhibits the proliferation of OcP, accelerating their differentiation into bone-resorbing OC, which ultimately cause osteoporosis. The possibility that TREM2 and β-catenin act along the same pathway is supported not only by the similar osteoporotic phenotypes of TREM2−/− and βcatΔ/Δ mice, but also by genetic evidence that simultaneous heterozygosity for TREM2- and β-catenin-null alleles results in osteoporosis, whereas no phenotype is observed in mice heterozygous for either of these alleles.. TREM2 may enhance M-CSF-induced activation of β-catenin by facilitating the recruitment of DAP12 to the receptor for M-CSF. In turn, DAP12 may activate Syk and Pyk2, which promote phosphorylation and nuclear ...
Levels of β-catenin and phospho-β-catenin (p-β-catenin), and their subcellular localization.In HB1.F3, β-catenin is mainly localized in nucleus (A), and p-
Vascular and neuronal networks share a similar branching morphology, and emerging evidence implicates common mechanisms in the formation of both systems. δ-Catenin is considered a neuronal catenin regulating neuron cell-cell adhesion and cell motility. Here, we report expression of δ-catenin in vascular endothelium, and show that deletion of only one allele of δ-catenin is sufficient to impair endothelial cell motility and vascular assembly in vitro and pathological angiogenesis in vivo, thereby inhibiting tumor growth and wound healing. In contrast, deletion of one or both allele of δ-catenin had no effects on hormone-induced physiological angiogenesis in the uterus. Molecular analysis confirmed a gene dosage effect of δ-catenin on Rho GTPase activity. Moreover, we show that inflammatory cytokines, but not angiogenic factors, regulate δ-catenin expression, and the levels of δ-catenin positively correlate to human lung cancers. Collectively, our data suggest that inflammation, commonly ...
The present invention relates to mounting and adhesion systems and methods for compact electronic modules. More particularly, the present invention relates to an apparatus and technique for mounting an electronic module that is capable of communicating with an interface unit on a one-way-bus.
gamma-catenin Antibody (F-2) is a monoclonal anti-γ-catenin antibody that detects m, r, and h γ-catenin by WB, IP, IF and ELISA.
β-Catenin is essential for embryonic development and required for cell renewal/regeneration in adult life. Cellular β-catenin exists in three different pools: membranous, cytoplasmic and nuclear. In...
Antibody Sampler Kit for studying Catenin-alphaE/Catenin-beta/E-Cadherin/N-Cadherin/P-Cadherin/R-cadherin in the Adhesion research area.
Mutations in the Wnt/-catenin pathway occur in most colorectal cancers (CRCs), and these mutations lead to increased nuclear accumulation of the -catenin transcriptional co-activator. element within the first intron of the gene to drive expression in CRC cells. As such, reducing -catenin expression in CRC cells using shRNAs leads to decreased mRNA and protein levels. …Read More. ...
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Absence of β-catenin does not affect spontaneous or glucocorticoid-induced death of thymocytes. (a) Percentage of surviving thymocytes gated on CD45.1+ (WT don
The canonical WNT-β-catenin pathway is essential for self-renewal, growth and survival of AML stem/blast progenitor cells (BPCs). Deregulated WNT signaling inhibits degradation of β-catenin, causing increased nuclear translocation and co-factor activity of β-catenin with the transcriptional regulator TCF4/LEF1 in AML BPCs. Here, we determined the pre-clinical anti-AML activity of the ...
Three different types of catenins seem to exist: alpha, beta, and gamma. Alpha-catenins are proteins of about 100 kDa which are ... in alpha-catenin, of the repeated domain and of the proline-rich segment. Vinculin InterPro: IPR000633 Alpha-catenin InterPro: ... Alpha-catenins are evolutionary related to vinculin. Catenins are proteins that associate with the cytoplasmic domain of a ... Lottspeich F, Eckerskorn C, Herrenknecht K, Ozawa M, Lenter M, Kemler R (1991). "The uvomorulin-anchorage protein alpha catenin ...
Janssens B, Staes K, van Roy F (1999). "Human alpha-catulin, a novel alpha-catenin-like molecule with conserved genomic ... Merdek KD, Nguyen NT, Toksoz D (2004). "Distinct Activities of the α-Catenin Family, α-Catulin and α-Catenin, on β-Catenin- ... "Association of Lbc Rho guanine nucleotide exchange factor with alpha-catenin-related protein, alpha-catulin/CTNNAL1, supports ... "Association of Lbc Rho guanine nucleotide exchange factor with alpha-catenin-related protein, alpha-catulin/CTNNAL1, supports ...
... has been shown to interact with: Beta-catenin, CDH1, Catenin (cadherin-associated protein), alpha 1, Nephrin and ... "The uvomorulin-anchorage protein alpha catenin is a vinculin homologue". Proceedings of the National Academy of Sciences of the ... Reynolds AB, Daniel JM, Mo YY, Wu J, Zhang Z (Jun 1996). "The novel catenin p120cas binds classical cadherins and induces an ...
... has been shown to interact with Beta-catenin and liprin-alpha-1. GRCh38: Ensembl release 89: ENSG00000142949 - Ensembl, ... increases beta-catenin-E-cadherin association, and decreases beta-catenin-sensitive transcription". Cancer Res. 61 (4): 1671-7 ... as well as in the control of beta-catenin signaling. An increased expression level of this protein was found in the insulin- ... "Geldanamycin abrogates ErbB2 association with proteasome-resistant beta-catenin in melanoma cells, ...
"Identification of plakoglobin domains required for association with N-cadherin and alpha-catenin". The Journal of Biological ... N-cadherins interact with catenins to play an important role in learning and memory (For full article see Cadherin-catenin ... Wahl JK, Kim YJ, Cullen JM, Johnson KR, Wheelock MJ (May 2003). "N-cadherin-catenin complexes form prior to cleavage of the ... February 1999). "delta-catenin, an adhesive junction-associated protein which promotes cell scattering". The Journal of Cell ...
Nicholl ID, Matsui T, Weiss TM, Stanley CB, Heller WT, Martel A, Farago B, Callaway DJ, Bu Z (Aug 21, 2018). "Alpha-catenin ... Bu Z, Cook J, Callaway DJ (Sep 2001). "Dynamic regimes and correlated structural dynamics in native and denatured alpha- ...
"Alpha-catenin structure and nanoscale dynamics in solution and in complex with F-actin". Biophysical Journal. 115 (4): 642-654 ...
Nicholl ID, Matsui T, Weiss TM, Stanley CB, Heller WT, Martel A, Farago B, Callaway DJ, Bu Z (Aug 21, 2018). "Alpha-catenin ... Intracellular domains of cadherins are also highly conserved, as they bind to proteins called catenins, forming catenin- ... No catenin is present in desmosomes as intracellular domains of desmosomal cadherins interact with desmosomal plaque proteins, ... which in turn promotes the assembly of adherens junctions by binding to the cadherin-catenin complexes that then form at the ...
2000). "Coordinate recruitment of E-cadherin and ALCAM to cell-cell contacts by alpha-catenin". Biochem. Biophys. Res. Commun. ...
"Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin". J. Cell Biol. 130 (1): 67 ... cellular domains of E-cadherin molecules bind to the actin cytoskeleton via the adaptor proteins alpha-catenin and beta-catenin ...
Regulatory proteins include p-120 catenin, α {\displaystyle \alpha } -catenin, β {\displaystyle \beta } -catenin, and vinculin ... α {\displaystyle \alpha } -catenin is engaged by p120-catenin complex, where vinculin is recruited to take a role in indirect ... Binding of p-120 catenin and β {\displaystyle \beta } -catenin to the homodimer increases the stability of the classical ... alpha } -catenin and vinculin. Cadherins behave as both receptors and ligands for other molecules. During development, their ...
"The LIM protein Ajuba is recruited to cadherin-dependent cell junctions through an association with alpha-catenin". J. Biol. ...
"Identification of the domain of alpha-catenin involved in its association with beta-catenin and plakoglobin (gamma-catenin)". ... "A mutation in alpha-catenin disrupts adhesion in clone A cells without perturbing its actin and beta-catenin binding activity ... "The APC protein and E-cadherin form similar but independent complexes with alpha-catenin, beta-catenin, and plakoglobin". The ... "The APC protein and E-cadherin form similar but independent complexes with alpha-catenin, beta-catenin, and plakoglobin". The ...
"Involvement of ZO-1 in cadherin-based cell adhesion through its direct binding to alpha catenin and actin filaments". The ... "Involvement of ZO-1 in cadherin-based cell adhesion through its direct binding to alpha catenin and actin filaments". The ... a MAGUK family member associated with tight as well as adherens junctions with a binding affinity to occludin and alpha catenin ... a MAGUK family member associated with tight as well as adherens junctions with a binding affinity to occludin and alpha catenin ...
ALP interacts with: alpha actinin-2 beta-catenin GRCh38: Ensembl release 89: ENSG00000154553 - Ensembl, May 2017 "Human PubMed ... Alpha actinin-2 is however not required for targeting ALP to Z-lines. Studies in ALP knockout mice have shown that ALP ... The PDZ domain of ALP binds to alpha actinin-2, specifically to its spectrin-like repeats. The PDZ domain is a motif composed ... Studies have shown that ALP is present at the first stage of myofibrilogenesis where it is bound to alpha actinin-2, and this ...
Catenin-associated Fer and Fyn tyrosine kinases regulate beta-catenin Tyr-142 phosphorylation and beta-catenin-alpha-catenin ... Pokutta S, Weis WI (Mar 2000). "Structure of the dimerization and beta-catenin-binding region of alpha-catenin". Molecular Cell ... "A truncated beta-catenin disrupts the interaction between E-cadherin and alpha-catenin: a cause of loss of intercellular ... "A mutation in alpha-catenin disrupts adhesion in clone A cells without perturbing its actin and beta-catenin binding activity ...
Catenin-associated Fer and Fyn tyrosine kinases regulate beta-catenin Tyr-142 phosphorylation and beta-catenin-alpha-catenin ... Kihara T, Shimohama S, Sawada H, Honda K, Nakamizo T, Shibasaki H, Kume T, Akaike A (April 2001). "alpha 7 nicotinic receptor ... Martinez MC, Ochiishi T, Majewski M, Kosik KS (July 2003). "Dual regulation of neuronal morphogenesis by a delta-catenin- ...
Together with an alpha-beta catenin complex, the cadherin can bind to the microfilaments of the cytoskeleton of the cell. This ... The β-catenin-α-catenin linked complex at the adherens junctions allows for the formation of a dynamic link to the actin ... Brembeck FH, Rosário M, Birchmeier W (February 2006). "Balancing cell adhesion and Wnt signaling, the key role of β-catenin". ...
Mammalian embryos undergo compaction around the 8-cell stage where E-cadherins as well as alpha and beta catenins are expressed ...
November 2002). "Association of Lbc Rho guanine nucleotide exchange factor with alpha-catenin-related protein, alpha-catulin/ ... AKAP13 has been shown to interact with: CTNNAL1 Estrogen receptor alpha GNA12 GNA13 PRKAR2A Second messenger system G protein- ... to increase the rate of GTP hydrolysis of G12/G13 alpha proteins. The A-kinase anchor proteins (AKAPs) are a group of ...
Catenin-associated Fer and Fyn tyrosine kinases regulate beta-catenin Tyr-142 phosphorylation and beta-catenin-alpha-catenin ...
... nuclear import of alpha-catenin by the beta-catenin/Tcf complex". Experimental Cell Research. 255 (2): 207-20. doi:10.1006/excr ... It is a member of the TCF family that can form a bipartite transcription factor (β-catenin/TCF) alongside β-catenin. Bipartite ... TCF7L2 is downstream of the WNT/β-catenin pathways. The activation of the WNT/β-catenin pathways have been associated ... Tang W, Dodge M, Gundapaneni D, Michnoff C, Roth M, Lum L (July 2008). "A genome-wide RNAi screen for Wnt/beta-catenin pathway ...
Adherens junctions are composed primarily of E-cadherin, alpha and beta catenins and other proteins such as actin and myosin. ... Vezatin is a major component of the cadherin-catenin complex that is critical to the formation and maintenance of adherens ... E-cadherins on separate cells use calcium based interactions to bind, whilst the catenins bind the cadherins to the actin ... These results taken together suggest that vezatin plays a crucial role in the cadherin-catenin complex which is essential for ...
... such as p120-catenin, beta-catenin and alpha-catenin. Catenins, in turn, bind to the actin cytoskeleton. Binding of these ... Although p120 catenin is a potential substrate of PTPmu, others have suggested that the interaction between PTPmu and catenins ... In addition to catenins and cadherins, PTPmu dephosphorylates PIPKIγ90 and nectin-3 (PVRL3) to stabilize E-cadherin-based ... IQGAP1 is a scaffold for Rho family of GTPases, E-cadherin, beta-catenin and other proteins. IQGAP1 binding to Rho GTPases is ...
NR4A2 has been shown to interact with: Beta-catenin, Pituitary homeobox 3, Retinoic acid receptor alpha, and Retinoic acid ... Zhang L, Cen L, Qu S, Wei L, Mo M, Feng J, Sun C, Xiao Y, Luo Q, Li S, Yang X, Xu P (Apr 2016). "Enhancing Beta-Catenin ... and peroxisome proliferator-activated receptor alpha genes in schizophrenia and alcohol dependence: possible haplotype ... structure has shown that the binding surface of the ligand-binding domain is located on the grooves of the 11th and 12th alpha ...
... a MAGUK family member associated with tight as well as adherens junctions with a binding affinity to occludin and alpha catenin ... "Tyrosine phosphorylation and dissociation of occludin-ZO-1 and E-cadherin-beta-catenin complexes from the cytoskeleton by ...
... a MAGUK family member associated with tight as well as adherens junctions with a binding affinity to occludin and alpha catenin ... a MAGUK family member associated with tight as well as adherens junctions with a binding affinity to occludin and alpha catenin ...
Beta-catenin can also bind to alpha-catenin. Alpha-catenin participates in regulation of actin-containing cytoskeletal ... Catenin-associated Fer and Fyn tyrosine kinases regulate beta-catenin Tyr-142 phosphorylation and beta-catenin-alpha-catenin ... "A truncated beta-catenin disrupts the interaction between E-cadherin and alpha-catenin: a cause of loss of intercellular ... Loss of E-cadherin expression results in releasing β-catenin into the cytoplasm. Liberated β-catenin molecules may migrate into ...
Catenin-associated Fer and Fyn tyrosine kinases regulate beta-catenin Tyr-142 phosphorylation and beta-catenin-alpha-catenin ... "A truncated beta-catenin disrupts the interaction between E-cadherin and alpha-catenin: a cause of loss of intercellular ... increases beta-catenin-E-cadherin association, and decreases beta-catenin-sensitive transcription". Cancer Res. 61 (4): 1671-7 ... "The structure of the beta-catenin/E-cadherin complex and the molecular basis of diverse ligand recognition by beta-catenin". ...
Catenin-associated Fer and Fyn tyrosine kinases regulate beta-catenin Tyr-142 phosphorylation and beta-catenin-alpha-catenin ... alpha 1, Cortactin, FYN, MUC1, Nephrin, PSEN1, PTPN6, PTPRJ, PTPRM, VE-cadherin, YES1, and ZBTB33 Delta catenin Catenin CTNND2 ... p120, and called catenin delta-1 is a protein that in humans is encoded by the CTNND1 gene. This gene encodes a member of the ... "Entrez Gene: CTNND1 catenin (cadherin-associated protein), delta 1". Schackmann, R. C. J.; Tenhagen, M.; van de Ven, R. A. H.; ...
连环蛋白(英语:Catenin). *Alpha catenin. *Beta catenin *APC ...
Zhang F, Meng F, Li H, Dong Y, Yang W, Han A (September 2011). "Suppression of retinoid X receptor alpha and aberrant β-catenin ... α-catenin β-catenin δ-catenin γ-catenin All but α-catenin contain armadillo repeats. Several types of catenins work with N- ... The first two catenins that were identified became known as α-catenin and β-catenin. A-catenin can bind to β-catenin and can ... B-catenin binds the cytoplasmic domain of some cadherins. Additional catenins such as γ-catenin and δ-catenin have been ...
Vallenius T، Luukko K، Mäkelä TP (2000). "CLP-36 PDZ-LIM protein associates with nonmuscle alpha-actinin-1 and alpha-actinin-4 ... "Identification of a Wnt/Dvl/beta-Catenin --, Pitx2 pathway mediating cell-type-specific proliferation during development.". ... binds to alpha-actinin-1 and associates with actin filaments and stress fibers in activated platelets and endothelial cells.". ...
... nuclear translocation of androgen receptor complex with beta-catenin and T-cell factor 4 may bypass canonical Wnt signaling to ... norepinephrine due to lack of alpha-2 receptor negative feedback and decreased fat accumulation due to epinephrine/ ... increase beta adrenergic receptors while decreasing alpha adrenergic receptors- which results in increased levels of ...
... and stabilize beta-catenin.[20] Mutant of presenilin-1 that reduces the ability to stabilize beta-catenin complex leads to ... They showed that cleavage by alpha- and beta- secretase was still normal without the presence of presenilin-1. Meanwhile, the ... beta-catenin binding. • protein binding. • calcium channel activity. • aspartic-type endopeptidase activity. • endopeptidase ... "Presenilin couples the paired phosphorylation of beta-catenin independent of axin: implications for beta-catenin activation in ...
In colorectal cancer, for example, mutations in APC, axin, or β-catenin promote β-catenin stabilization and transcription of ... has been shown to enhance apoptosis caused by chemotherapeutic drugs through autocrine-secreted tumor necrosis factor alpha ( ... This destruction complex normally triggers β-catenin phosphorylation, inducing its degradation. De-regulation of the autocrine ... the Wnt signaling pathway leads to stabilization of β-catenin through inactivation of a protein complex containing the tumor ...
Catenins. *Alpha catenin. *Beta catenin *APC. *Plakoglobin (gamma catenin). *Delta catenin. *GAN ...
Two types of PDGFRs have been identified: alpha-type and beta-type PDGFRs.[8] The alpha type binds to PDGF-AA, PDGF-BB and PDGF ... Though PDGF is synthesized,[3] stored (in the alpha granules of platelets),[4] and released by platelets upon activation, it is ... "Chimeric alpha- and beta-platelet-derived growth factor (PDGF) receptors define three immunoglobulin-like domains of the alpha ... alpha PDGF) receptor for PDGF focus forming activity chemotaxis, or growth". J. Biol. Chem. 270 (13): 7033-6. doi:10.1074/jbc. ...
Catenins. *Alpha catenin. *Beta catenin *APC. *Plakoglobin (gamma catenin). *Delta catenin. *GAN ...
alpha sarcoglycan Left side-normal muscle /right side LGMD2. There is a variety of research under way targeted at various forms ... Catenin. *APC *Gardner's syndrome. *Familial adenomatous polyposis. *plakoglobin (Naxos syndrome). *GAN (Giant axonal ...
"A degradation signal located in the C-terminus of p21WAF1/CIP1 is a binding site for the C8 alpha-subunit of the 20S ...
Catenins. *Alpha catenin. *Beta catenin *APC. *Plakoglobin (gamma catenin). *Delta catenin. *GAN ...
Beta-catenin can also bind to alpha-catenin. Alpha-catenin participates in regulation of actin-containing cytoskeletal ... Catenin-associated Fer and Fyn tyrosine kinases regulate beta-catenin Tyr-142 phosphorylation and beta-catenin-alpha-catenin ... "A truncated beta-catenin disrupts the interaction between E-cadherin and alpha-catenin: a cause of loss of intercellular ... gamma-catenin binding. • beta-catenin binding. • GTPase activating protein binding. • metal ion binding. • cell adhesion ...
β-catenin Calcium Channel Voltage-dependent calcium channel (VDCC) Allows the rapid influx of calcium during an action ...
Gs alpha subunit. stimulates AC, raises cAMP, stimulates beta catenin and Glycogen synthase kinase 3 ... those containing the Gs alpha subunit, Gi alpha subunit, Gq alpha subunit and/or G12 subunit;[2][4] and f) signaling pathways ... Gs alpha subunit. activates AC, increases cAMP, raises Ca2+ Prostaglandin DP2 receptor. DP2. PGD2,,PGF2α=PGE2,PGI2=TXA2[7]. ?. ... Gq alpha subunit. stimulates PLC, IP3, & PKC; raises Ca2+ Prostacyclin I2 receptor. IP. PGI2,,PGD2=PGE2=PGF2α,TXA2[13]. ...
Catenins. *Alpha catenin. *Beta catenin *APC. *Plakoglobin (gamma catenin). *Delta catenin. *GAN ... Two heavy chains dimerize through the tail domain forming a long alpha-helical coiled-coil rod composed of typical heptad ... Sajid M, Hu Z, Lele M, Stouffer GA (May 2000). "Protein complexes involving alpha v beta 3 integrins, nonmuscle myosin heavy ... phosphorylation of the 20 KDa light chain unfolds the molecule to produce a globular head region followed by an extended alpha- ...
Catenins. *Alpha catenin. *Beta catenin *APC. *Plakoglobin (gamma catenin). *Delta catenin. *GAN ... Sun N, Critchley DR, Paulin D, Li Z, Robson RM (May 2008). "Identification of a repeated domain within mammalian alpha-synemin ... "The talin rod IBS2 alpha-helix interacts with the beta3 integrin cytoplasmic tail membrane-proximal helix by establishing ...
"Binding of GDNF and neurturin to human GDNF family receptor alpha 1 and 2. Influence of cRET and cooperative interactions". J. ...
Flores ER, Halder G (2011). "Stem cell proliferation in the skin: alpha-catenin takes over the hippo pathway". Sci Signal 4 ( ... Zhang F, Meng F, Li H, Dong Y, Yang W, Han A (September 2011). "Suppression of retinoid X receptor alpha and aberrant β-catenin ... "Links between alpha-catenin, NF-kappaB, and squamous cell carcinoma in skin". Proc. Natl. Acad. Sci. U.S.A. 103 (7): 2322-7. ... "Hyperproliferation and defects in epithelial polarity upon conditional ablation of alpha-catenin in skin". Cell 104 (4): 605-17 ...
These include the canonical Wnt/β-catenin pathway, Wnt/calcium pathway, and planar cell polarity (PCP) pathway.[3] Mutations in ... The structure of this domain is known and is composed mainly of alpha helices. This domain contains ten conserved cysteines ...
Chen D, Li M, Luo J, Gu W (April 2003). "Direct interactions between HIF-1 alpha and Mdm2 modulate p53 function". The Journal ... Bálint E, Bates S, Vousden KH (July 1999). "Mdm2 binds p73 alpha without targeting degradation". Oncogene. 18 (27): 3923-9. doi ...
Catenins. *Alpha catenin. *Beta catenin *APC. *Plakoglobin (gamma catenin). *Delta catenin. *GAN ... An amphipathic alpha-helix in the divergent regulatory domain of ankyrin-b interacts with the molecular co-chaperone Hdj1/Hsp40 ... folds as an amphipathic alpha helix is required for normal levels of sodium-calcium exchanger, sodium potassium ATPase and ...
PHD action normally requires oxygen and alpha-ketoglutarate as cosubstrates and ferrous iron and ascorbate as cofactors. ... "Succinate links TCA cycle dysfunction to oncogenesis by inhibiting HIF-alpha prolyl hydroxylase". Cancer Cell. 7 (1): 77-85. ... "Cell-permeating alpha-ketoglutarate derivatives alleviate pseudohypoxia in succinate dehydrogenase-deficient cells". Mol. Cell ...
β-catenin actives the Wnt pathway necessary for Muller glial proliferation Rod precursor differentiation is another mechanism ... robust Muller glia proliferation and subsequent neuronal differentiation has been seen using the alpha 7 nAChR agonist, PNU- ... "β-catenin/Wnt signaling controls progenitor fate in the developing and regenerating zebrafish retina". Neural Development. 2012 ...
This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein ... Through its effects on E-cadherin and beta-catenin, which form the adherens junction and promote cell adhesion, NDRG1 also ...
Catenins. *Alpha catenin. *Beta catenin *APC. *Plakoglobin (gamma catenin). *Delta catenin. *GAN ... It has an extended lever arm consisting of five calmodulin binding IQ motifs followed by a single alpha helix (SAH)[28] Myosin ...
beta-catenin-TCF complex assembly. • transcription, DNA-templated. • transcription from RNA polymerase II promoter. • G1/S ... "Myc-nick: a cytoplasmic cleavage product of Myc that promotes alpha-tubulin acetylation and cell differentiation". Cell. 142 ...
AMPKα1 (PRKAA1; protein kinase, AMP-activated, alpha 1 catalytic subunit). *AMPKα2 (protein kinase, AMP-activated, alpha 2 ... Somatic mutations of LKB1 and beta-catenin genes in gastrointestinal polyps from patients with Peutz-Jeghers syndrome. „Cancer ... Complexes between the LKB1 tumor suppressor, STRAD alpha/beta and MO25 alpha/beta are upstream kinases in the AMP-activated ... motif as a regulatory mechanism for the interaction of GPR motifs with Gi alpha. „J Biol Chem". 278. 26, s. 23217-20, 2003. DOI ...
The head domain of vinculin associates to E-cadherin via α-catenin, β-catenin, and γ-catenin. The tail domain of vinculin binds ... alpha skeletal muscle actin in the nuclei of mouse myoblasts,[111]. *cytoplasmic gamma actin and also alpha smooth muscle actin ... alpha, beta, and gamma have been identified. The alpha actins, found in muscle tissues, are a major constituent of the ... "Human actin genes are single copy for alpha-skeletal and alpha-cardiac actin but multicopy for beta- and gamma-cytoskeletal ...
Alpha-catenins are proteins of about 100 kDa which are evolutionary related to vinculin [PMID: 1924379]. Catenins are proteins ... alpha, beta, and gamma. In terms of their structure the most significant differences are the absence, in alpha-catenin, of the ... The uvomorulin-anchorage protein alpha catenin is a vinculin homologue.. Proc. Natl. Acad. Sci. U.S.A. 88 9156-60 1991 ... The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to ...
... alpha-2-catenin (also called alpha-N-catenin) CTNNA3, alpha-3-catenin (also called alpha-T-catenin) Catenin alpha+Catenin at ... Three alpha-catenin genes are expressed in humans: CTNNA1, alpha-1-catenin (also called alpha-E-catenin) CTNNA2, ... Alpha catenin exhibits significant protein dynamics. The amino acid sequence of alpha-catenin has sequence similarity to that ... "Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin". J. Cell Biol. 130 (1): 67 ...
... cadherins cytoplasmic domain binds p120 catenin and beta-catenin, which in turn binds alpha-catenin. To elucidate the roles of ... plasma membrane targeted or untargeted p120 catenin, alpha-catenin and beta-catenin and tested their ability to rescue the loss ... Right panel represents the scheme of chimeric fusion constructs of α-catenin (A), β-catenin (B) or p120 catenin (C) with either ... Signaling function of alpha-catenin in microtubule regulation.. Shtutman M1, Chausovsky A, Prager-Khoutorsky M, Schiefermeier N ...
αE-catenin, also known as Catenin alpha-1 is a protein that in humans is encoded by the CTNNA1 gene. αE-catenin is highly ... αE-catenin has been shown to interact with: APC, Beta-catenin, CDH1, CDH2, CDH3 Plakoglobin, and VE-cadherin. Alpha catenin ... Catenin-associated Fer and Fyn tyrosine kinases regulate beta-catenin Tyr-142 phosphorylation and beta-catenin-alpha-catenin ... "Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin". J. Cell Biol. 130 (1): 67 ...
Compare Anti-alpha Catenin Antibody Products from leading suppliers on Biocompare. View specifications, prices, citations, ...
Compare catenin alpha 1 ELISA Kits from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and ... catenin alpha 1 ELISA Kits. The ELISA (enzyme-linked immunosorbent assay) is a well-established antibody-based tool for ... Mouse/Human/Rat Alpha 1+2 Catenin ELISA Kit (Cell-Based ELISA) ... Your search returned 152 catenin alpha 1 ELISA ELISA Kit across ...
Browse our Catenin alpha Antibody catalog backed by our Guarantee+. ... Catenin alpha Antibodies. We offer Catenin alpha Antibodies for use in common research applications: Immunohistochemistry, ... Our Catenin alpha Antibodies can be used in a variety of model species: Human, Mouse, Xenopus. Use the list below to choose the ... Each Catenin alpha Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you ...
Catenin alpha-1Imported. ,p>Information which has been imported from another database using automatic procedures.,/p> ,p>,a ... tr,E5RJP7,E5RJP7_HUMAN Catenin alpha-1 (Fragment) OS=Homo sapiens GN=CTNNA1 PE=1 SV=1 ...
IPR036723. Alpha-catenin/vinculin-like_sf. IPR001033. Alpha_catenin. IPR030046. CTNNA2. IPR006077. Vinculin/catenin. IPR000633. ... IPR036723. Alpha-catenin/vinculin-like_sf. IPR001033. Alpha_catenin. IPR030046. CTNNA2. IPR006077. Vinculin/catenin. IPR000633. ... Belongs to the vinculin/alpha-catenin family.Curated. Phylogenomic databases. evolutionary genealogy of genes: Non-supervised ... sp,Q5R416,CTNA2_PONAB Catenin alpha-2 OS=Pongo abelii GN=CTNNA2 PE=2 SV=3 ...
catenin alpha-2. Names. alpha-N-catenin. alpha-catenin-related protein. cadherin-associated protein, related. cancer/testis ... HIV-1 gp41 and alpha-catenin co-localize in gp160 and GST-alpha-catenin expressing cells; the leucine zipper-like domain (gp160 ... CTNNA2 catenin alpha 2 [Homo sapiens] CTNNA2 catenin alpha 2 [Homo sapiens]. Gene ID:1496 ... catenin alpha 2provided by HGNC. Primary source. HGNC:HGNC:2510 See related. Ensembl:ENSG00000066032 MIM:114025 Gene type. ...
In adherens junctions, alpha-catenin links the cadherin-beta-catenin complex to the actin-based cytoskeleton. alpha-catenin is ... In adherens junctions, alpha-catenin links the cadherin-beta-catenin complex to the actin-based cytoskeleton. alpha-catenin is ... A chimera consisting of the alpha-catenin-binding region of beta-catenin linked to the amino terminus of alpha-catenin 57-264 ... The crystal structure of the alpha-catenin dimerization domain, residues 82-279, shows that alpha-catenin dimerizes through ...
... catenin is a novel actin-binding and -bundling protein and support a model in which alpha 1(E)-catenin is responsible for ... Recombinant human beta-catenin can simultaneously bind to the alpha-catenin/actin complex but does not bind actin directly. ... Alpha 1(E)-catenin is an actin-binding and -bundling protein mediating the attachment of F-actin to the membrane adhesion ... Alpha 1(E)-catenin is an actin-binding and -bundling protein mediating the attachment of F-actin to the membrane adhesion ...
... including alpha-catenin. We identified a subtype of alpha-catenin, termed alpha N-catenin, which is associated with N-cadherin ... including alpha-catenin. We identified a subtype of alpha-catenin, termed alpha N-catenin, which is associated with N-cadherin ... which express E-cadherin and beta-catenin but neither alpha- nor alpha N-catenin, with alpha N-catenin cDNA. While parental PC9 ... cDNA transfection experiments showed that alpha N-catenin can also bind with E-cadherin. To investigate the role of alpha N- ...
... alpha-catenin. The alpha-catenin bound directly to the N-terminal region of EspB, both in solid (overlay assay) and solution ( ... The EspB protein of enterohaemorrhagic Escherichia coli interacts directly with alpha-catenin Cell Microbiol. 2002 Apr;4(4):213 ... We conclude that EspB recruits alpha-catenin at the EHEC adherence site by direct interaction, and that the recruitment of ...
Home › Online Presentations › [Illinois] CMMB IGERT & M-CNTC Annual Symposium: Alpha-Catenin in Cadherin Mechanotransduction › ... Illinois] CMMB IGERT & M-CNTC Annual Symposium: Alpha-Catenin in Cadherin Mechanotransduction By Hamid Tabdili ... Hamid Tabdili (2013), "[Illinois] CMMB IGERT & M-CNTC Annual Symposium: Alpha-Catenin in Cadherin Mechanotransduction," http:// ...
Alpha-catenin-dependent recruitment of the centrosomal protein CAP350 to adherens junctions allows epithelial cells to acquire ... We also uncover a central role of α-catenin in global cytoskeleton remodelling, in which it acts not only on actin but also on ... By two-hybrid screening, we identified a direct interaction of CAP350 with the adhesion protein α-catenin that was further ... Block of epithelial cadherin (E-cadherin)-mediated cell-cell adhesion or α-catenin depletion prevented CAP350 localisation at ...
The Wnt/beta-catenin signalling pathway contributed to resistance to IFN-alpha/5-FU therapy. Immunohistochemical analysis ... The results indicate that activation of Wnt/beta-catenin signalling pathway induces chemoresistance to IFN-alpha/5-FU therapy ... Activation of Wnt/beta-catenin signalling pathway induces chemoresistance to interferon-alpha/5-fluorouracil combination ... The aim of this study was to examine the mechanism of anti-proliferative effects of IFN-alpha/5-FU therapy and search for a ...
... cadherins cytoplasmic domain binds p120 catenin and beta-catenin, which in turn binds alpha-catenin. To elucidate the roles of ... plasma membrane targeted or untargeted p120 catenin, alpha-catenin and beta-catenin and tested their ability to rescue the loss ... Only membrane targeting of alpha-catenin led to a significant increase in microtubule length and density in centrosome-free ... Expression of non-membrane-targeted alpha-catenin produced only a slight effect, while both membrane-targeted and non-targeted ...
2 Catenin in Adherent Cell Cultures. It is a Cell-Based assay which can detect Alpha 1+2 Catenin ... 2 Catenin ELISA Kit LS-F1858 is a 96-Well enzyme-linked immunosorbent assay for the Qualitative detection of Human Alpha 1+ ... LS-F1858 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Qualitative detection of Human Alpha 1+2 Catenin in ... Mouse/Human/Rat Alpha 1+2 Catenin ELISA Kit (Cell-Based ELISA) - LS-F1858 ...
alpha N Catenin Antibody 14362-1-AP has been identified with IF, IP, WB, ELISA. 14362-1-AP detected 102 kDa band in SH-SY5Y ... alpha N Catenin Antibody 0 Publications. Rabbit Polyclonal, Catalog number: 14362-1-AP ... SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14362-1-AP (alpha N Catenin antibody) at dilution of 1: ... SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14362-1-AP (alpha N Catenin antibody) at dilution of 1: ...
Alpha N-catenin, a Neuronal Subtype Catenin Exhibits Fiber Cell Specific Distribution; Regulation of the Alpha N-catenin: N- ... Alpha N-catenin, a Neuronal Subtype Catenin Exhibits Fiber Cell Specific Distribution; Regulation of the Alpha N-catenin: N- ... Alpha N-catenin, a Neuronal Subtype Catenin Exhibits Fiber Cell Specific Distribution; Regulation of the Alpha N-catenin: N- ... The coimmunoprecipitation of αN-catenin with Prx and AnkB, in coexistence of αN-catenin with N-cadherin, β-catenin, Prx and ...
S27D binds to beta-catenin and dissociates alpha-catenin from beta-catenin. (A) HUVEC lysates were incubated with S27D and pull ... Beta-catenin connects VE-cadherin to alpha-catenin. This VE-cadherin-catenin complex is believed to dynamically control ... Western blot was immunostained for beta-catenin (I.B.), which showed that S27D dissociates beta-catenin from alpha-catenin, but ... Alpha-catenin is a molecular switch that binds E-cadherin-beta-catenin and regulates actin-filament assembly. Cell. 2005;123: ...
Isolated cDNAs encoded a 110-kD protein with 60% identity to mouse alpha E-catenin, and this protein was termed D alpha-catenin ... Here, we report the cloning of a Drosophila alpha-catenin gene by low stringent hybridization with a mouse alpha E-catenin ... Identification of a Drosophila homologue of alpha-catenin and its association with the armadillo protein. H Oda , H Oda ... alpha-Catenins are a group of proteins associated with cadherins, and this association is crucial for the cadherins function. ...
Rabbit polyclonal to alpha 1 Catenin; Cadherin-associated protein; Alpha E-catenin; NY-REN-13 antigen Polyclonal \ 18-272- ... Product name : alpha 1 Catenin - Rabbit polyclonal to alpha 1 Catenin; Cadherin-associated protein; Alpha E-catenin; NY-REN-13 ... alpha 1 Catenin - Rabbit polyclonal to alpha 1 Catenin; Cadherin-associated protein; Alpha E-catenin; NY-REN-13 antigen ... alpha 1 Catenin - Rabbit polyclonal to alpha 1 Catenin; Cadherin-associated protein; Alpha E-catenin; NY-REN-13 antigen ...
Alpha-1 catenin - Cadherin-associated protein; Alpha E-catenin; NY-REN-13 antigen Polyclonal \ 15-288-22836 for more molecular ... Alpha E-catenin; NY-REN-13 antigen Polyclonal. Related products : Alpha-1 catenin - Cadherin-associated protein; Alpha E- ... Product name : Alpha-1 catenin - Cadherin-associated protein; Alpha E-catenin; NY-REN-13 antigen Polyclonal ... Alpha-1 catenin - Cadherin-associated protein; Alpha E-catenin; NY-REN-13 antigen Polyclonal antibody storage GENTAUR ...
Alpha-catenin is encoded by the CTNNA1 gene in human. Alpha-catenin belongs to a protein family known as catenins. ... Catenins also form the plaque of adherens junction. Alpha-catenin is known to interact with beta-catenin, vinculin, and alpha- ... Alpha-catenin in the developing skin. Alpha-catenin proteins are found in the cell junctions of the epithelial cell layers in ... Alpha-catenin in the developing kidney. Alpha-catenin proteins are found in the cell junctions of the renal tubules from an ...
ALPHA 1 CATENIN/CTNNA1 information: Molecular Weight: 100071 MW; Subcellular Localization: Isoform 1: Cytoplasm, ... Polyclonal antibody for ALPHA 1 CATENIN/CTNNA1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive ... Background for Catenin alpha-1. CTNNA1, also known as Catenin alpha-1 or Catenin (cadherin-associated protein), alpha 1, is a ... Catenin alpha-1;Alpha E-catenin;Cadherin-associated protein;Renal carcinoma antigen NY-REN-13;CTNNA1;. ...
... beta-catenin/CTNNB1, alpha-catenin/CTNNA1 and p120 catenin/CTNND1 (PubMed:28051089). UniProt ... Possible component of an E-cadherin/ catenin adhesion complex together with E-cadherin/CDH1 and beta-catenin/CTNNB1 or gamma- ... The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to ... In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to ...
Catenin Alpha 1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene ... Loss of alpha-catenin decreases the strength of single E-cadherin bonds between human cancer cells. (PMID: 19458087) Bajpai S ... catenin adhesion complex together with E-cadherin/CDH1 and beta-catenin/CTNNB1 or gamma-catenin/JUP; the complex is located to ... CTNNA1 (Catenin Alpha 1) is a Protein Coding gene. Diseases associated with CTNNA1 include Macular Dystrophy, Patterned, 2 and ...
  • Alpha-catenin functions as the primary protein link between cadherins and the actin cytoskeleton. (wikipedia.org)
  • It has been observed that when alpha-catenin is not in a molecular complex with beta-catenin, it dimerizes and functions to regulate actin filament assembly[citation needed], possibly by competing with Arp2/3 protein. (wikipedia.org)
  • Alpha catenin exhibits significant protein dynamics. (wikipedia.org)
  • The uvomorulin-anchorage protein alpha catenin is a vinculin homologue. (ebi.ac.uk)
  • αE-catenin, also known as Catenin alpha-1 is a protein that in humans is encoded by the CTNNA1 gene. (wikipedia.org)
  • Human αE-catenin protein is 100.0 kDa and 906 amino acids. (wikipedia.org)
  • Catenins (α,β,and γ (also known as plakoglobin)) were originally identified in complex with E-cadherin, an epithelial cell adhesion protein. (wikipedia.org)
  • Functional αE-catenin is required for normal embryonic development, as a mutation eliminating the C-terminal 1/3 of the protein resulting in a complete loss-of-function phenotype showed disruption of the trophoblast epithelium and arrested development at the blastocyst stage. (wikipedia.org)
  • Recombinant fragments encompassing the amino-terminal 228 residues of alpha 1(E)-catenin or the carboxyl-terminal 447 residues individually bind actin in cosedimentation assays with reduced affinity compared with the full-length protein, and neither fragment bundles actin. (pnas.org)
  • Collectively these data indicate that alpha 1 (E)-catenin is a novel actin-binding and -bundling protein and support a model in which alpha 1(E)-catenin is responsible for organizing and tethering actin filaments at the zones of E-cadherin-mediated cell-cell contact. (pnas.org)
  • To determine the actual role of EspB in this process, we tried to identify the EspB binding partner of the host cell protein, using a yeast two-hybrid assay, and obtained a cytoskeletal-associated protein, alpha-catenin. (nih.gov)
  • Alpha-catenin-dependent recruitment of the centrosomal protein CAP350 to adherens junctions allows epithelial cells to acquire a columnar shape. (sigmaaldrich.com)
  • By two-hybrid screening, we identified a direct interaction of CAP350 with the adhesion protein α-catenin that was further confirmed by co-immunoprecipitation experiments. (sigmaaldrich.com)
  • To determine the potential involvement of Periaxin (Prx), a PDZ domain containing protein, in regulation of N-cadherin/Catenin-based cell adhesive interactions in lens fibers. (arvojournals.org)
  • VE-cadherin is part of a protein complex comprising p120-catenin and beta-catenin as intracellular partners. (ijbs.com)
  • It associates to the armadillo-family proteins beta- and gamma-catenin that bind the actin-binding protein alpha-catenin [ 1 , 2 ]. (ijbs.com)
  • Identification of a Drosophila homologue of alpha-catenin and its association with the armadillo protein. (rupress.org)
  • Isolated cDNAs encoded a 110-kD protein with 60% identity to mouse alpha E-catenin, and this protein was termed D alpha-catenin. (rupress.org)
  • These included the armadillo product which was known to be a Drosophila homologue of beta-catenin, another cadherin-associated protein in vertebrates, and a 150-kD glycoprotein. (rupress.org)
  • Alpha-catenin belongs to a protein family known as catenins. (bicellscientific.com)
  • CTNNA1 (Catenin Alpha 1) is a Protein Coding gene. (genecards.org)
  • Depletion of HeLa cell brefeldin A-inhibited guanine nucleotide-exchange factors (BIG)1 and/or BIG2 impaired β-catenin S675 phosphorylation and its transcriptional effects, as well as ADP-ribosylation factor activation-enhanced phospholipase D activity and vesicular trafficking, both required for restoration of transcriptional activation by appropriate BIG protein overexpression in depleted cells. (pnas.org)
  • This entry represents the beta catenins and homologues, plakoglobin and the Drosophila Armadillo protein [ PMID: 2261639 ], which are implicated in cell adhesion and Wnt signalling [ PMID: 9425166 ]. (ebi.ac.uk)
  • Beta catenin family proteins contain several ARM repeats, sequences of approximately 50 amino acids involved in protein-protein interactions. (ebi.ac.uk)
  • Cell-cell adhesion in epithelial cells is mediated by the cell surface protein E-cadherin in association with alpha- and beta-catenin, which link E-cadherin to the cytoskeleton. (umassmed.edu)
  • Expression of beta1 integrins in GE11 cells resulted in a decrease in cadherin and alpha-catenin protein levels accompanied by their redistribution from the cytoskeleton-associated fraction to the detergent-soluble fraction. (nih.gov)
  • Regulation of alpha-catenin protein levels by beta1 integrins is likely to play a role in the morphological transition, since overexpression of alpha-catenin in GE11 cells before beta1 prevented the disruption of intercellular adhesions and cell scattering. (nih.gov)
  • αE-catenin is an actin-binding protein associated with the E-cadherin-based adherens junction that regulates cell-cell adhesion. (biologists.org)
  • The cytoplasmic domain of classical cadherins binds β-catenin and p120-catenin ( Shapiro and Weis, 2009 ), and β-catenin in turn binds the actin-binding protein α-catenin that connects the AJ with the actin cytoskeleton ( Benjamin and Nelson, 2008 ). (biologists.org)
  • Auf www.antikoerper-online.de finden Sie aktuell 39 Catenin (Cadherin-Associated Protein), alpha 2 (CTNNA2) Antikörper von 15 unterschiedlichen Herstellern. (antikoerper-online.de)
  • beta-catenin is an intracellular protein associated with the actin cytoskeleton of a cell. (redorbit.com)
  • In this process there are multiple protein-complexes involved which interact with beta-catenin and E-cadherin. (redorbit.com)
  • also called δ-catenin) binds via a postsynaptic density-95 (PSD-95)/discs large/zona occludens-1 (PDZ) interaction to AMPA receptor (AMPAR)-binding protein (ABP) and the related glutamate receptor (GluR)-interacting protein (GRIP), two multi-PDZ proteins that bind the GluR2 and GluR3 AMPAR subunits. (jneurosci.org)
  • The presence of adherens junctions and the associated protein β-catenin are requirements for the development of glucose-stimulated insulin secretion (GSIS) in β-cells. (portlandpress.com)
  • Toward that goal, Wirtz, who also is associate director of the Johns Hopkins Institute for NanoBioTechnology, led a multi-institution team that focused on alpha-catenin, a small protein that floats in the cytoplasm, the gel-like material that surrounds the nucleus inside a cell. (bio-medicine.org)
  • Integrity of this complex is positively regulated by beta -catenin phosphorylation by casein kinase II, and dephosphorylation by protein tyrosine phosphatases. (genome.jp)
  • β-Catenin is an important protein in these events and regulates cadherin-mediated cell adhesion and the recruitment of pre- and postsynaptic proteins in an activity-dependent fashion. (deepdyve.com)
  • Three alpha-catenin genes are expressed in humans: CTNNA1, alpha-1-catenin (also called alpha-E-catenin) CTNNA2, alpha-2-catenin (also called alpha-N-catenin) CTNNA3, alpha-3-catenin (also called alpha-T-catenin) Catenin alpha+Catenin at the US National Library of Medicine Medical Subject Headings (MeSH) Cooper, Geoffrey M. (2000). (wikipedia.org)
  • Alpha-catenin is encoded by the CTNNA1 gene in human. (bicellscientific.com)
  • Rabbit IgG polyclonal antibody for Catenin alpha-1(CTNNA1) detection. (bosterbio.com)
  • In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. (rcsb.org)
  • Component of an cadherin:catenin adhesion complex composed of at least of CDH26 , beta-catenin/ CTNNB1 , alpha-catenin/ CTNNA1 and p120 catenin/ CTNND1 (PubMed:28051089). (rcsb.org)
  • In adherens junctions, alpha-catenin links the cadherin-beta-catenin complex to the actin-based cytoskeleton. (rcsb.org)
  • These actions involve additional proteins, including alpha- and beta-catenin (or plakoglobin) and p120, as well as linkage to the cortical actin cytoskeleton. (pnas.org)
  • We also uncover a central role of α-catenin in global cytoskeleton remodelling, in which it acts not only on actin but also on MT reorganisation during epithelial morphogenesis. (sigmaaldrich.com)
  • Catenins are the linker proteins that connect adherens junction to the underlying cytoskeleton. (bicellscientific.com)
  • Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. (rcsb.org)
  • The name "catenin" was originally selected ('catena' means 'chain' in Latin) because it was suspected that catenins might link cadherins to the cytoskeleton. (wikipedia.org)
  • β-catenin acts by anchoring the actin cytoskeleton to the junctions, and may possibly aid in contact inhibition signaling within the cell. (wikipedia.org)
  • Multifunctional β-catenin is an essential component of intercellular adherens junctions (AJs) that link cell surface cadherin and actin cytoskeleton via interactions with both. (pnas.org)
  • and therefore, it was assumed that αE-catenin statically links the cadherin-catenin complex to the actin cytoskeleton ( Gates and Peifer, 2005 ). (rupress.org)
  • Additionally, other actin-associated proteins bind αE-catenin and could provide an indirect link to the actin cytoskeleton. (rupress.org)
  • Together, these observations suggest that the catenins play a central role in regulating functional interactions between cadherins and the actin cytoskeleton. (biologists.org)
  • Alpha N-catenin, a linker between cadherin adhesion receptors and the actin cytoskeleton, is essential for stabilizing dendritic spines in rodent hippocampal neurons in culture. (antikoerper-online.de)
  • E- cadherins bind to beta-catenin to form a complex which can interact both with neighboring cells to form bonds, and with the cytoskeleton of the cell. (redorbit.com)
  • The alpha- catenin and beta-catenin then form a complex to link the actin filaments of the cytoskeleton and the E-cadherins. (redorbit.com)
  • Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton (By similarity). (drugbank.ca)
  • To this end, we have analysed the role of α-Catenin, a key molecule linking E-Cadherin-based adhesion and the actomyosin cytoskeleton, during Drosophila embryonic dorsal closure, by studying a newly developed allelic series. (semanticscholar.org)
  • β-Catenin links N-cadherin to the actin cytoskeleton through interactions with α-catenin (Ozawa et al. (deepdyve.com)
  • It has been reported that the actin binding proteins vinculin and alpha-actinin can bind to alpha-catenin. (wikipedia.org)
  • In addition to actin, vinculin interacts with other structural proteins such as talin and alpha-actinins. (ebi.ac.uk)
  • Alpha-catenins are proteins of about 100 kDa which are evolutionary related to vinculin [ PMID: 1924379 ]. (ebi.ac.uk)
  • Catenins are proteins that associate with the cytoplasmic domain of a variety of cadherins. (ebi.ac.uk)
  • however, aside from vinculin, αE-catenin has no homology to established actin-binding proteins. (wikipedia.org)
  • To elucidate the roles of the cadherin-associated proteins in regulating microtubule dynamics, we prepared GFP-tagged, plasma membrane targeted or untargeted p120 catenin, alpha-catenin and beta-catenin and tested their ability to rescue the loss of microtubule mass caused by centrosomal removal in the poorly adhesive cell line CHO-K1. (nih.gov)
  • Left panel presents Western blots showing expression of endogenous α-catenin (A), β-catenin (B) and p120ctn (C) and exogenous transfected EGFP-derivatives of corresponding proteins and their membrane-targeted IL2R fusion constructs. (nih.gov)
  • The function of cadherin cell adhesion molecules is thought to be regulated by a group of cytoplasmic proteins, including alpha-catenin. (nih.gov)
  • The expression, distribution and colocalization of αN-catenin with different cell adhesion, cytoskeletal and membrane proteins in mouse lens tissue was determined either by cDNA microarray, immunoblot or immunofluorescence analyses. (arvojournals.org)
  • Together, these proteins form the so-called cadherin-catenin complex. (ijbs.com)
  • alpha-Catenins are a group of proteins associated with cadherins, and this association is crucial for the cadherins' function. (rupress.org)
  • Alpha-catenin proteins are found in the cell junctions of the mucosal epithelium in the small intestine from an embryonic mouse at E15.5. (bicellscientific.com)
  • This gene encodes a member of the catenin family of proteins that play an important role in cell adhesion process by connecting cadherins located on the plasma membrane to the actin filaments inside the cell. (genecards.org)
  • Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells. (wikipedia.org)
  • Multifunctional β-catenin, with critical roles in both cell-cell adhesion and Wnt-signaling pathways, was among HeLa cell proteins coimmunoprecipitated by antibodies against brefeldin A-inhibited guanine nucleotide-exchange factors 1 and 2 (BIG1 or BIG2) that activate ADP-ribosylation factors (Arfs) by accelerating the replacement of bound GDP with GTP. (pnas.org)
  • Direct interaction of BIG1 N-terminal sequence with β-catenin was confirmed using yeast two-hybrid assays and in vitro synthesized proteins. (pnas.org)
  • Depletion of BIG1 and/or BIG2 or overexpression of guanine nucleotide-exchange factor inactive mutant, but not wild-type, proteins interfered with β-catenin trafficking, leading to accumulation at perinuclear Golgi structures. (pnas.org)
  • However, in almost all cases, the molecular affinities and stoichiometries of these additional proteins in the cadherin-catenin complex have not been defined. (rupress.org)
  • CONCLUSIONS: These data demonstrate that correction of a defect in the cadherin/catenin cell-cell adhesion complex, often found in poorly differentiated and highly invasive tumors, facilitates increased cell-cell adhesion and retards tumor cell migration on basement membrane and stromal proteins. (umassmed.edu)
  • Our results indicate that beta1 integrins regulate the polarity and motility of epithelial cells by the induction of intracellular molecular events involving a downregulation of alpha-catenin function and the activation of the Rho-like G proteins Rac1 and RhoA. (nih.gov)
  • Depletion of αE-catenin also caused deep cells to have protracted plasma membrane blebbing, and a defect in plasma membrane recruitment of ERM proteins that are involved in controlling membrane-to-cortex attachment and membrane blebbing. (biologists.org)
  • The cytoplasmatic tail of the E-cadherin molecule binds to the proteins of the catenin family: p120-catenin, beta-catenin, and beta-catenin. (redorbit.com)
  • The amino acid sequence of alpha-catenin has sequence similarity to that of vinculin. (wikipedia.org)
  • The N-terminus of αE-catenin binds β-catenin or γ-catenin/plakoglobin, and the C-terminus binds actin directly or indirectly via vinculin or α-actinin. (wikipedia.org)
  • This was further characterized by disorganization of intercalated disc structures and mitochondria, as well as compensatory increases in β-catenin and decreases in localization of cadherin and vinculin at intercalated discs. (wikipedia.org)
  • Alpha-catenin is known to interact with beta-catenin, vinculin, and alpha-actinin. (bicellscientific.com)
  • however, it is likely that α-catenin acts in concert with vinculin to bind to actin and help stabilize the junctions. (wikipedia.org)
  • α-catenin, vinculin, and F-actin in strengthening E-cadherin cell-cell adhesions and mechanosensing. (ebi.ac.uk)
  • alphaE-catenin recruits vinculin to adherens junctions more effectively than alphaN-catenin, partly because of its higher affinity for actin filaments. (antikoerper-online.de)
  • We have investigated the mechanism for the effects of vinculin and found that the reduced surface E-cadherin expression could be rescued by introduction of vinculin, but not of a vinculin A50I substitution mutant that is defective for β-catenin binding. (biologists.org)
  • These findings suggest that an interaction between β-catenin and vinculin is crucial for stabilizing E-cadherin at the cell surface. (biologists.org)
  • This was confirmed by analyzing a β-catenin mutant that fails to bind vinculin. (biologists.org)
  • The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. (ebi.ac.uk)
  • B-catenin binds the cytoplasmic domain of some cadherins. (wikipedia.org)
  • Several types of catenins work with N-cadherins to play an important role in learning and memory (For full article, see Cadherin-catenin complex in learning and memory). (wikipedia.org)
  • Catenins play roles in cellular organization and polarity long before the development and incorporation of Wnt signaling pathways and cadherins. (wikipedia.org)
  • The primary mechanical role of catenins is connecting cadherins to actin filaments, specifically in these adhesion junctions of epithelial cells. (wikipedia.org)
  • Although the importance of cadherins in mammary morphogenesis is widely accepted, the role of p120-catenin (also known as catenin delta 1) in this process remains to be investigated. (biologists.org)
  • Here, we report the cloning of a Drosophila alpha-catenin gene by low stringent hybridization with a mouse alpha E-catenin probe. (rupress.org)
  • While the pathway is very detailed and not completely understood, in general, when Wnt is not present, GSK-3B (a member of the pathway) is able to phosphorylate β-catenin as a result of a complex formation that includes β-catenin, AXIN1, AXIN2, APC (a tumor suppressor gene product), CSNK1A1, and GSK3B. (wikipedia.org)
  • These gene products are important in determining cell fates during normal development and in maintaining homeostasis, or they can lead to de-regulated growth in disorders like cancer by responding to mutations in β-catenin, APC or Axin, each of which can lead to this de-regulated β-catenin level stabilization in cells. (wikipedia.org)
  • We propose that interaction of β-catenin-bound BIG1 with BIG2 and its associated PKA complex, simultaneously or sequentially, are required for phosphorylation of S675, with nuclear entry of activated β-catenin followed by its acceleration of T-cell factor/lymphoid enhancer factor family gene transcription. (pnas.org)
  • The gene encoding alpha-T-catenin, CTNNA3, is positioned within a region on chromosome 10, showing strong evidence of linkage to Alzheimer's disease (AD), and is therefore a good positional candidate gene for this disorder. (ox.ac.uk)
  • We show that T3-TR1 controls the transcription of the beta-catenin gene in an epithelial cell-autonomous way. (archives-ouvertes.fr)
  • In addition, we show that the regulation of the beta-catenin gene is direct, as TR binds in vitro and in chromatin in vivo to a specific thyroid hormone-responsive element present in intron 1 of this gene. (archives-ouvertes.fr)
  • This is the first report concerning in vivo transcriptional control of the beta-catenin gene. (archives-ouvertes.fr)
  • Alpha-T-catenin (CTNNA3) gene was identified as a risk variant for toluene diisocyanate-induced asthma by genome-wide association analysis. (cdc.gov)
  • Abe K and Takeichi M 2007 NMDA-receptor activation induces calpain-mediated β-Catenin cleavages for triggering gene expression. (springer.com)
  • 2001 The chromatin remodelling factor Brg-1 interacts with beta-catenin to promote target gene activation. (springer.com)
  • 2003) Fine mapping of the α-T catenin gene to a quantitative trait locus on chromosome 10 in late-onset Alzheimer's disease pedigrees. (springer.com)
  • 2003) Assessment of the CTNNA3 gene encoding human alpha T-catenin regarding its involvement in dilated cardiomyopathy. (springer.com)
  • Mutations in the β-catenin gene can cause cognitive disability and autism, with life-long consequences. (deepdyve.com)
  • 2012). This contribution of β-catenin to brain development and synaptic function gained additional interest in recent years because mutations in the β-catenin gene were found to cause intellectual disability (Tucci et al. (deepdyve.com)
  • 2000). NMDA-receptor (NMDAR) activation in hippocampal neurons was found to result in nuclear translocation of β-catenin and increased TCF/LEF-dependent gene expression (Abe and Takeichi 2007). (deepdyve.com)
  • Using the primary visual cortex (V1) as a model, we asked whether cortical function or plasticity are affected when the β-catenin gene is disrupted. (deepdyve.com)
  • Activation of Wnt/beta-catenin signalling pathway induces chemoresistance to interferon-alpha/5-fluorouracil combination therapy for hepatocellular carcinoma. (sigmaaldrich.com)
  • The Wnt/beta-catenin signalling pathway contributed to resistance to IFN-alpha/5-FU therapy. (sigmaaldrich.com)
  • The results indicate that activation of Wnt/beta-catenin signalling pathway induces chemoresistance to IFN-alpha/5-FU therapy and suggest that Ep-CAM is a potentially useful marker for resistance to such therapy, especially in IFNAR2-positive cases. (sigmaaldrich.com)
  • The crystal structure of this chimera reveals the interaction between alpha- and beta-catenin, and provides a basis for understanding adherens junction assembly. (rcsb.org)
  • Catenins also form the plaque of adherens junction. (bicellscientific.com)
  • Mutational analysis supports a core role for Drosophila α-catenin in adherens junction function. (semanticscholar.org)
  • Each Catenin alpha Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. (novusbio.com)
  • Genetic polymorphisms of CTNNA3 (catenin alpha 3, alpha-T catenin) were significantly associated with the TDI-induced asthma phenotype (5.84 x 10(-6) for rs10762058, 1.41 x 10(-5) for rs7088181, 2.03 x 10(-5) for rs4378283). (cdc.gov)
  • Recently, a genome-wide association study (GWAS) conducted in Korean subjects identified four CTNNA3 (alpha-T catenin) single nucleotide polymorphisms (SNPs) (rs10762058, rs7088181, rs1786929, and rs4378283) associated with diisocyanate-induced occupational asthma (DA). (cdc.gov)
  • αE-catenin binds the cell-cell adhesion complex of E-cadherin and β-catenin (β-cat) and regulates filamentous actin (F-actin) dynamics. (rupress.org)
  • In cells, to test whether αE-catenin regulates actin dynamics independently of the cadherin complex, the cytosolic αE-catenin pool was sequestered to mitochondria without affecting overall levels of αE-catenin or the cadherin-catenin complex. (rupress.org)
  • These results demonstrate that the cytosolic pool of αE-catenin regulates actin dynamics independently of cell-cell adhesion. (rupress.org)
  • These studies raised the possibility that αE-catenin regulates F-actin independently of the E-cadherin cell-cell adhesion complex. (biologists.org)
  • β-Catenin-mediated signalling regulates cancer cell metabolism and is associated with tumour-initiating cells in multiple malignancies. (springer.com)
  • Beiter K, Hiendlmeyer E, Brabletz T, Hlubek F, Haynl A, Knoll C, Kirchner T and Jung A 2005 beta-Catenin regulates the expression of tenascin-C in human colorectal tumors. (springer.com)
  • β-Catenin in the Adult Visual Cortex Regulates NMDA-Receptor Function and Visual Responses Saiepour, M Hadi;Min, Rogier;Kamphuis, Willem;Heimel, J Alexander;Levelt, Christiaan N 2018-04-01 00:00:00 Abstract The formation, plasticity and maintenance of synaptic connections is regulated by molecular and electrical signals. (deepdyve.com)
  • Biallelic loss of human CTNNA2, encoding αN-catenin, leads to ARP2/3 complex overactivity and disordered cortical neuronal migration. (nih.gov)
  • αE-catenin, along with β-catenin and plakoglobin form distinct complexes with N-cadherin that are involved in forming cell-cell contacts and differentiation of cardiomyocytes. (wikipedia.org)
  • Catenin-N-cadherin complexes are apparently necessary for and precede the first cell to cell contact, precursory to gap junction formation. (wikipedia.org)
  • The anchorage of cadherin-catenin complexes to actin filaments by αE-catenin is regulated by tyrosine phosphorylation. (wikipedia.org)
  • These complexes, which help regulate cell growth in addition to creating and maintaining epithelial layers, are known as adherens junctions and they typically include at least cadherin, β-catenin, and α-catenin. (wikipedia.org)
  • α-catenin participates in the formation and stabilization of adherens junctions by binding to β-catenin-cadherin complexes in the cell. (wikipedia.org)
  • Our data suggest that Nemo serves as a conserved molecular link between core PCP factors and E-cadherin-β-catenin complexes, promoting cell motility. (nature.com)
  • α-Catenin stabilises Cadherin-Catenin complexes and modulates actomyosin dynamics to allow pulsatile apical contraction. (semanticscholar.org)
  • Signaling function of alpha-catenin in microtubule regulation. (nih.gov)
  • Peripheral mechanisms take central stage in microtubule dynamics: commentary on "Signaling function of alpha-catenin in microtubule regulation" by Shtutman et al. (nih.gov)
  • Regulation of a novel alphaN-catenin splice variant in schizophrenic smokers. (nih.gov)
  • The catenins are thought to play an important role in the regulation of VE-cadherin function. (ijbs.com)
  • Kikuchi A. Regulation of beta-catenin signaling in the Wnt pathway. (ebi.ac.uk)
  • This is parallel to positive regulation of proliferation-controlling genes such as type D cyclins and c-myc, known targets of the Wnt/-beta-catenin. (archives-ouvertes.fr)
  • However, recent studies have caused a re-evaluation of α-catenin regulation and function. (biologists.org)
  • Together this identifies phosphorylation of β-catenin at Ser552 as part of a cell signalling mechanism linking nutrient and hormonal regulation of β-catenin to modulation of insulin secretory capacity of β-cells and indicates this phosphorylation event is regulated downstream of EPAC and PAK1 in β-cells. (portlandpress.com)
  • 2014), and changes in the regulation of β-catenin stability by glycogen synthase kinase 3β (GSK3β) have been implicated in schizophrenia (Mao et al. (deepdyve.com)
  • In adherens junctions, cadherin's cytoplasmic domain binds p120 catenin and beta-catenin, which in turn binds alpha-catenin. (nih.gov)
  • The cytoplasmic tail binds beta-catenin, which in turn binds alpha-catenin. (genome.jp)
  • Choose from our Catenin alpha polyclonal antibodies. (novusbio.com)
  • F9 cells were genetically engineered to lack β-catenin, resulting in increased association of plakoglobin with E-cadherin. (wikipedia.org)
  • In F9 cells lacking both β-catenin and plakoglobin, very little E-cadherin and α-catenin accumulated at the cell surface. (wikipedia.org)
  • Mice lacking plakoglobin have cell adhesion defects in many tissues, although β-catenin substitutes for plakoglobin at many cellular junctions. (wikipedia.org)
  • Signaling and adhesion activities of mammalian beta-catenin and plakoglobin in Drosophila. (ebi.ac.uk)
  • The armadillo homologs beta-catenin and plakoglobin are differentially expressed during early development of Xenopus laevis. (ebi.ac.uk)
  • It has been suggested that alpha-catenin does not bind with high affinity to both actin filaments and the E-cadherin-beta-catenin complex at the same time. (wikipedia.org)
  • Recombinant human beta-catenin can simultaneously bind to the alpha-catenin/actin complex but does not bind actin directly. (pnas.org)
  • cDNA transfection experiments showed that alpha N-catenin can also bind with E-cadherin. (nih.gov)
  • A-catenin can bind to β-catenin and can also bind actin. (wikipedia.org)
  • In vitro, binding of αE-catenin to the E-cadherin-β-cat complex lowers αE-catenin affinity for F-actin, and αE-catenin alone can bind F-actin and inhibit Arp2/3 complex-mediated actin polymerization. (rupress.org)
  • Beta-catenin forms a cadherin/beta-catenin/alphaE-catenin complex that can tether the tripartite adhesion complex and regulate actin dynamics [ PMID: 23739176 ]. (ebi.ac.uk)
  • In the cortex, alpha-catenin is switched from alphaE-catenin in the VZ to alphaN-catenin II in the intermediate zone (IMZ). (antikoerper-online.de)
  • A chimera consisting of the alpha-catenin-binding region of beta-catenin linked to the amino terminus of alpha-catenin 57-264 behaves as a monomer in solution, as expected, since beta-catenin binding disrupts the alpha-catenin dimer. (rcsb.org)
  • Three-dimensional structure of the armadillo repeat region of beta-catenin. (ebi.ac.uk)
  • We demonstrate a direct interaction between F-actin and alpha (E)-catenin, an activity not shared by either the cytoplasmic domain of E-cadherin or beta-catenin. (pnas.org)
  • We conclude that EspB recruits alpha-catenin at the EHEC adherence site by direct interaction, and that the recruitment of alpha-catenin is essential for EHEC-induced A/E lesion formation. (nih.gov)
  • Immunoprecipitation in conjunction with mass spectrometry and immunoblot analysis of lens homogenates was performed to assess the interaction of catenins with Prx and Ankyrin-B (AnkB) in lens tissue. (arvojournals.org)
  • In order to study the importance of the interaction between alpha- and beta-catenin in TEM, we used a cell-permeable version of the peptide encoding the binding site of alpha-catenin for beta-catenin (S27D). (ijbs.com)
  • The data show that S27D interferes with the interaction between alpha- and beta-catenin and induces a reversible decrease in electrical resistance of the endothelial monolayer. (ijbs.com)
  • Behrens J, von Kries JP, Kühl M, Bruhn L, Wedlich D, Grosschedl R and Birchmeier W 1996 Functional interaction of beta-catenin with the transcription factor LEF-1. (springer.com)
  • The interaction of N-cadherin with β-catenin is strengthened by synaptic activity (Murase et al. (deepdyve.com)
  • F9 embryonal carcinoma cells are similar to the P19 cells shown in Figure 1 and normally have cell-to-cell adhesion mediated by E-cadherin with β-catenin bound to the cytoplasmic domain of E-cadherin. (wikipedia.org)
  • Recent studies identified additional E-cadherin-independent roles of αE-catenin in regulating plasma membrane dynamics and cell migration. (biologists.org)
  • However, little is known about the roles of αE-catenin in these different cellular processes in vivo during early vertebrate development. (biologists.org)
  • Thus, αE-catenin appears to play roles in maintaining cell-cell adhesion and suppressing membrane dynamics associated with cell migration. (biologists.org)
  • We provide evidence that Girdin binds to β-catenin that plays important roles in E-cadherin-mediated cell-cell adhesion. (nii.ac.jp)
  • Three different types of catenins seem to exist: alpha, beta, and gamma. (ebi.ac.uk)
  • In contrast, sequestration of cytosolic αE-catenin to the plasma membrane reduces membrane dynamics. (rupress.org)
  • Here, we examined the functions of αE-catenin in cell-cell adhesion, cell migration and plasma membrane dynamics during morphogenetic processes that drive epiboly in early Danio rerio (zebrafish) development. (biologists.org)
  • Significantly, depletion of both E-cadherin and αE-catenin suppressed plasma membrane blebbing. (biologists.org)
  • By contrast, β-catenin is required to facilitate cadherin transport to the plasma membrane. (biologists.org)
  • α-catenin β-catenin δ-catenin γ-catenin All but α-catenin contain armadillo repeats. (wikipedia.org)
  • Genetic and molecular studies indicate that Nemo binds both the core PCP factor complex of Strabismus-Prickle, as well as the E-cadherin-β-catenin (E-cadherin-Armadillo in Drosophila ) complex. (nature.com)
  • [ 1 ] [ 2 ] En Drosophila a proteína homóloga denomínase armadillo . (wikipedia.org)
  • [ 7 ] Pero axiña se descubriu que a proteína de Drosophila armadillo (implicada na mediación de efectos morfoxénicos de Wingless/Wnt ) é homóloga da β-catenina de mamíferos, non só en estrutura senón tamén en función. (wikipedia.org)
  • Lampugnani and co-workers showed that tyrosine phosphorylation of VE-cadherin and associated catenins is increased in loosely confluent endothelial monolayers, whereas the phosphorylation is diminished in tightly confluent monolayers [ 9 ]. (ijbs.com)
  • Ozawa and Kemler showed that increased levels of tyrosine phosphorylation, induced by pervanadate promote the dissociation of alpha-catenin from the cadherin-catenin complex [ 10 ]. (ijbs.com)
  • Following phosphorylation of the N-terminal Ser and Thr residues of β-catenin, BTRC promotes its ubiquitination, which causes it to be degraded by the TrCP/SKP complex. (wikipedia.org)
  • Transcriptional coactivator β-catenin promotes cell proliferation in development and tumorigenesis, via actions enhanced by PKA-catalyzed phosphorylation of its C-terminal region S675. (pnas.org)
  • Inferring a requirement for BIG1 and/or BIG2 AKAP sequence in PKA modification of β-catenin and its effect on transcription activation, we confirmed dependence of S675 phosphorylation and transcription coactivator function on BIG2 AKAP-C sequence. (pnas.org)
  • Nemo and β-catenin then act synergistically to promote rotation, which is mediated in vivo by Nemo's phosphorylation of β-catenin. (nature.com)
  • Evidence indicates that modulation of β-catenin function in response to changes in glucose levels can modulate the levels of insulin secretion from β-cells but the role of β-catenin phosphorylation in this process has not been established. (portlandpress.com)
  • We find that a Ser552Ala version of β-catenin attenuates glucose-stimulated insulin secretion indicating a functional role for Ser552 phosphorylation of β-catenin in insulin secretion. (portlandpress.com)
  • Both glucose and GLP-1 stimulated phosphorylation of the serine 552 residue on β-catenin. (portlandpress.com)
  • We find that reduction in PAK1 levels using siRNA attenuates both glucose and GLP-1 stimulated phosphorylation of β-catenin Ser552 and the effects of these on insulin secretion in β-cell models. (portlandpress.com)
  • The integrity of the cadherin-catenin complex is negatively regulated by phosphorylation of beta-catenin by receptor tyrosine kinases (RTKs) and cytoplasmic tyrosine kinases (Fer, Fyn, Yes, and Src), which leads to dissociation of the cadherin-catenin complex. (genome.jp)
  • Changes in the phosphorylation state of beta-catenin affect cell-cell adhesion, cell migration and the level of signaling beta-catenin. (genome.jp)
  • Note that blood vessels in the submucosa also express alpha-catenin. (bicellscientific.com)
  • Keratinocytes engineered to not express alpha-catenin have disrupted cell adhesion and activated NF-κB. (wikipedia.org)
  • Although p120-catenin (p120) is crucial for E-cadherin function, ablation experiments in epithelial tissues from different organ systems reveal markedly different effects. (biologists.org)
  • Immunohistochemical analysis showed positive epithelial cell adhesion molecule (Ep-CAM) expression, the target molecule of Wnt/beta-catenin signalling, only in non-responders. (sigmaaldrich.com)
  • αE-catenin also plays a role in tumor metastasis and skin cell function. (wikipedia.org)
  • Though αE-catenin exhibits substantial expression in cardiac muscle, αE-catenin is most well known for role in metastasizing tumor cells. (wikipedia.org)
  • As Wnt/beta-catenin plays a crucial role in intestinal tumorigenesis, our observations open a new perspective on the study of TRs as potential tumor inducers. (archives-ouvertes.fr)
  • The objectives of this study were to investigate the expression levels of E-cadherin and β-catenin in meningioma with both temporal and spatial information, in order to determine their pathological significance in tumor invasion, formation of peritumoral edema, and postoperative recurrence. (pubmedcentralcanada.ca)
  • 2004 Synergy between LRH-1 and β-catenin induces G1 cyclin-mediated cell proliferation. (springer.com)
  • van Buul JD, van Alphen FP, Hordijk PL. The presence of alpha-catenin in the VE-cadherin complex is required for efficient transendothelial migration of leukocytes. (ijbs.com)
  • This VE-cadherin-catenin complex is believed to dynamically control endothelial cell-cell junctions and to regulate the passage of leukocytes, although not much is known about the role of alpha- and beta-catenin during the process of transendothelial migration (TEM). (ijbs.com)
  • In conclusion, our results show for the first time that the association of alpha-catenin with the cadherin-catenin complex is required for efficient leukocyte TEM. (ijbs.com)
  • Moreover, their results indicated that the binding of alpha-catenin to the cadherin-catenin complex is a prerequisite for proper functioning cadherin-catenin complex, since an E-cadherin-alpha-catenin chimera was insensitive to pervanadate treatment leaving E-cadherin trans-interactions intact, whereas cells expressing the normal cadherin-catenin complex, including beta-catenin, showed loss of cell-cell contact after pervanadate treatment [ 10 ]. (ijbs.com)
  • On the other hand, when Wnt is present, GSK-3B is displaced from the previously mentioned complex, causing β-catenin to not be phosphorylated, and thus not ubiquitinated. (wikipedia.org)
  • Three different forms of catenin (designated alpha, beta and gamma) comprise the cytoplasmic domain of the cadherin cell-cell adhesion complex [ PMID: 7945318 ]. (ebi.ac.uk)
  • Crystal structure of a beta-catenin/Tcf complex. (ebi.ac.uk)
  • Role of the E-cadherin/alpha-catenin complex in modulating cell-cell a" by Ellen C. Breen, Glenn D. Steele Jr. et al. (umassmed.edu)
  • Stable tissue integrity during embryonic development relies on the function of the cadherin·catenin complex (CCC). (rcsb.org)
  • A crystal structure shows that the HMP-1·HMP-2 complex forms a five-helical bundle structure distinct from the structure of the mammalian α-catenin·β-catenin complex. (rcsb.org)
  • sGiannini A. L., Vivanco M. D. M., and Kypta R. M. (2000) α-Catenin inhibits β-catenin signaling by preventing formation of a β-catenin T-cell factor DNA complex. (springer.com)
  • contrast sensitivity, NMDA-receptor, ocular dominance plasticity, synaptic, visual cortex Introduction Calcium-dependent cell adhesion through the N-cadherin-β-catenin complex has been identified as an important factor in synapse morphology and plasticity during forebrain development, and interfering with this complex during development has a severe impact on synaptic transmission (Tang et al. (deepdyve.com)
  • Girdin depletion led to impaired cytoskeletal association of the β-catenin complex, which was accompanied by changes in the supracellular actin cytoskeletal organization. (nii.ac.jp)
  • Your search returned 780 alpha Catenin Antibodies across 44 suppliers. (biocompare.com)
  • We offer Catenin alpha Antibodies for use in common research applications: Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot. (novusbio.com)
  • Our Catenin alpha Antibodies can be used in a variety of model species: Human, Mouse, Xenopus. (novusbio.com)
  • Block of epithelial cadherin (E-cadherin)-mediated cell-cell adhesion or α-catenin depletion prevented CAP350 localisation at cell-cell junctions. (sigmaaldrich.com)
  • Levels of PKA-phosphorylated β-catenin S675 and β-catenin association with PKA, BIG1, and BIG2 were also diminished after BIG1/BIG2 depletion. (pnas.org)
  • We show that depletion of αE-catenin caused a defect in radial intercalation that was associated with decreased cell-cell adhesion, in a similar manner to E-cadherin depletion. (biologists.org)
  • Studies show that full-length αE-catenin binds and bundles F-actin in a superior fashion relative to individual N-terminal or C-terminal domains. (wikipedia.org)
  • Sedimentation assays and direct visualization by transmission electron microscopy reveal that alpha 1(E)-catenin binds and bundles F-actin in vitro with micromolar affinity at a catenin/G-actin monomer ratio of approximately 1:7 (mol/mol). (pnas.org)
  • αE-catenin specifically, not β- or γ-catenin, binds F-actin and organizes and tethers the filaments at regions of cell-cell contact. (wikipedia.org)
  • BrdU-based cell proliferation ELISA and cell cycle analysis showed that concurrent addition of BIO and IFN-alpha/5-FU significantly to hepatoma cell cultures reduced the inhibitory effects of the latter two on DNA synthesis and accumulation of cells in the S-phase. (sigmaaldrich.com)
  • LS-F1858 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Qualitative detection of Human Alpha 1+2 Catenin in samples of Adherent Cell Cultures. (lsbio.com)
  • The integrity of N-cadherin and αN-catenin-based cell adhesive interactions in the absence of Prx was determined using Prx null mouse lenses. (arvojournals.org)
  • αN-catenin coexists with N-cadherin, β-catenin, Prx and AnkB in the lens fiber cell membrane rafts as determined by proteomics analysis of lipid rafts derived from the porcine lens, and colocalizes with N-cadherin, Prx, AnkB, Actin and NrCAM in the mouse lens fibers. (arvojournals.org)
  • The coimmunoprecipitation of αN-catenin with Prx and AnkB, in coexistence of αN-catenin with N-cadherin, β-catenin, Prx and AnkB in membrane rafts , and impairment of αN-catenin membrane localization in the absence of Prx in lens fibers, collectively suggests a crucial role for Prx in formation and stabilization of N-cadherin/αN-catenin-based cell adhesive interactions. (arvojournals.org)
  • In addition, S27D co-localized with beta-catenin at cell-cell junctions. (ijbs.com)
  • Several papers have shown that beta- and alpha-catenin become transiently and locally dispersed from cell-cell contacts upon passage of leukocytes [ 11 , 12 ]. (ijbs.com)
  • Immunostaining analysis using a mAb to D alpha-catenin revealed that it was localized to cell-cell contact sites, expressed throughout development and present in a wide variety of tissues. (rupress.org)
  • These results strongly suggest that Drosophila has a cell adhesion machinery homologous to the vertebrate cadherin-catenin system. (rupress.org)
  • β-catenin is particularly interesting as it plays a dual role in the cell. (wikipedia.org)
  • For instance, when an epithelial layer is complete and the adherens junctions indicate that the cell is surrounded, β-catenin may play a role in telling the cell to stop proliferating, as there is no room for more cells in the area. (wikipedia.org)
  • While less attention is directed at α-catenin in studies involving cell adhesion, it is nonetheless an important player in cellular organization, function and growth. (wikipedia.org)
  • Sequestering cytosolic αE-catenin to mitochondria alters lamellipodia architecture and increases membrane dynamics and cell migration without affecting cell-cell adhesion. (rupress.org)
  • Studies of αE-catenin in vivo have also suggested functions independent of cadherin-mediated cell-cell adhesion. (rupress.org)
  • Induction of cell scattering by expression of beta1 integrins in beta1-deficient epithelial cells requires activation of members of the rho family of GTPases and downregulation of cadherin and catenin function. (nih.gov)
  • We suggest that during radial intercalation the activities of E-cadherin and αE-catenin in the maintenance of membrane-to-cortex attachment are balanced, resulting in stabilization of cell-cell adhesion and suppression of membrane blebbing, thereby enabling proper radial intercalation. (biologists.org)
  • β-Catenin is essential for embryonic development and required for cell renewal/regeneration in adult life. (springer.com)
  • 2002 Beta-catenin and TCF mediate cell positioning in the intestinal epithelium by controlling the expression of EphB/ephrinB. (springer.com)
  • ABSTRACT In this article, we show, using a mathematical multiscale model, how cell adhesion may be regulated by interactions between E-cadherin and beta-catenin and how the control of cell adhesion may be related to cell migration, to the epithelial- mesenchymal transition and to invasion in populations of eukaryotic cells. (redorbit.com)
  • The role of E- cadherin in the malfunction of cell-cell adhesion observed in colorectal cancer, and in the beta-catenin degradation system after mutations that affect the wnt-pathway, belong to the most studied examples (2-4). (redorbit.com)
  • Thus, our study identifies α-catenin binding to ZO-1 as a new mechanism for coupling the assembly of the epithelial barrier to cell-to-cell adhesion. (biologists.org)
  • Molecular investigations showed that the stable Slit3 repression-induced cell proliferation was associated with a higher expression of β-catenin and a repressed GSK3β activity. (deepdyve.com)
  • Cadherin may acts as a negative regulator of signaling beta-catenin as it binds beta-catenin at the cell surface and thereby sequesters it from the nucleus. (genome.jp)
  • Interactions between MUC1 and p120 catenin regulate dynamic features of cell adhesion, motility, and metastasis. (pubfacts.com)
  • Most studies on the role of β-catenin have been performed in the developing brain or by using neuronal cell cultures. (deepdyve.com)
  • β-catenin is also a transcriptional coactivator in several pathways, including Wnt-initiated signaling ( 1 , 2 ), which is critical in embryonic development and tissue homeostasis or pathology ( 2 , 3 ). (pnas.org)
  • This is associated with alterations F/G actin ratio but not the transcriptional activity of β-catenin. (portlandpress.com)
  • 2012). As part of the canonical Wnt-signaling pathway, β-catenin also acts as a transcriptional co-factor for TCF/LEF family of transcription factors (Barker et al. (deepdyve.com)
  • alphaN-catenin expression is low in the ventricular zone and high in the developing cortical plate. (antikoerper-online.de)
  • HMP-1, unlike its mammalian homolog α-catenin, is constitutively monomeric, and its binding affinity for HMP-2/β-catenin is higher than that of α-catenin for β-catenin. (rcsb.org)
  • When cells detach from one another, beta-catenin is released into the cytoplasm, targeted for degradation, and downregulated. (redorbit.com)
  • Moreover, Slit3-repression induced chemoresistance to sorafenib, oxaliplatin and 5-FU through impairment of β-catenin degradation and induction of cyclin D3 and survivin levels. (deepdyve.com)
  • Wnt signaling acts as a positive regulator of beta-catenin by inhibiting beta-catenin degradation, which stabilizes beta-catenin, and causes its accumulation. (genome.jp)
  • The beta-catenin structure has been determined [ PMID: 9298899 , PMID: 11136974 ]. (ebi.ac.uk)
  • During synapse formation and plasticity, N-cadherin distribution at the synapse is regulated by interactions with its major binding partner β-catenin. (deepdyve.com)
  • In vitro studies showed that activation of Wnt/beta-catenin signalling by glycogen synthesis kinase-3 inhibitor (6-bromoindirubin-3'-oxime (BIO)) induced chemoresistance to IFN-alpha/5-FU. (sigmaaldrich.com)
  • To investigate the role of alpha N-catenin, we transfected lung carcinoma PC9 cells, which express E-cadherin and beta-catenin but neither alpha- nor alpha N-catenin, with alpha N-catenin cDNA. (nih.gov)
  • Mice engineered to specifically have vascular endothelium cells deficient in β-catenin showed disrupted adhesion between vascular endothelial cells. (wikipedia.org)
  • 2007 Chromosomal instability by β-catenin/TCF transcription in APC or β-catenin mutant cells. (springer.com)
  • Brabletz T, Hlubek F, Spaderna S, Schmalhofer O, Hiendlmeyer E, Jung A and Kirchner T 2005 Invasion and metastasis in colorectal cancer: epithelial-mesenchymal transition, mesenchymal-epithelial transition, stem cells and β-catenin. (springer.com)
  • Catenins interact closely with E-cadherin molecules in cells, and are therefore logical candidate genes for mutation screening in HDGC families. (aacrjournals.org)
  • Alpha-catenin allows cells to recognize neighboring cells as "friends" almost immediately, leading to the creation of many strong bonds that are hard to break. (bio-medicine.org)
  • However, cancer cells, including those found in diffuse gastric cancer and lung cancer, possess dysfunctional alpha-catenin and form very weak bonds with their neighbors. (bio-medicine.org)
  • To better understand these bonding characteristics, Wirtz and his colleagues used a technique called atomic force microscopy to study single cells with and without functioning alpha-catenin. (bio-medicine.org)
  • Wirtz's team discovered that normal cells with properly functioning alpha-catenin formed bonds that were four times more stable than those without functional alpha-catenin, and these first bonds formed in less than 1 millisecond. (bio-medicine.org)
  • In contrast, cells without functional alpha-catenin formed weak bonds from the onset. (bio-medicine.org)
  • Wirtz suggested that if scientists could figure out a way to repair or replace the alpha-catenin dysfunction found in some cancer cells, it could lead to a therapy that thwarts the spread of cancer. (bio-medicine.org)
  • αN-catenin is expressed in a lens fiber-specific manner in both embryonic and adult mouse lenses, and distributes to both, the soluble and the membrane fractions. (arvojournals.org)