Allophanate Hydrolase: An enzyme that catalyzes the hydrolysis of allophanic acid to two molecules of ammonia plus two molecules of "active carbon dioxide". EC 3.5.1.54.Biuret: Used as feed supplement for sheep and cattle since it is a good non-protein nitrogen source. In strongly alkaline solution biuret gives a violet color with copper sulfate.AmidohydrolasesUrea: A compound formed in the liver from ammonia produced by the deamination of amino acids. It is the principal end product of protein catabolism and constitutes about one half of the total urinary solids.Allantoin: A urea hydantoin that is found in URINE and PLANTS and is used in dermatological preparations.Oxamic Acid: Amino-substituted glyoxylic acid derivative.Carbon-Nitrogen Ligases: Enzymes that catalyze the joining of two molecules by the formation of a carbon-nitrogen bond. EC 6.3.UreohydrolasesGATA Transcription Factors: A family of transcription factors that contain two ZINC FINGER MOTIFS and bind to the DNA sequence (A/T)GATA(A/G).Search Engine: Software used to locate data or information stored in machine-readable form locally or at a distance such as an INTERNET site.Information Storage and Retrieval: Organized activities related to the storage, location, search, and retrieval of information.Barth Syndrome: Rare congenital X-linked disorder of lipid metabolism. Barth syndrome is transmitted in an X-linked recessive pattern. The syndrome is characterized by muscular weakness, growth retardation, DILATED CARDIOMYOPATHY, variable NEUTROPENIA, 3-methylglutaconic aciduria (type II) and decreases in mitochondrial CARDIOLIPIN level. Other biochemical and morphological mitochondrial abnormalities also exist.Tripterygium: A plant genus of the family CELASTRACEAE that is a source of triterpenoids and diterpene epoxides such as triptolide.Medication Adherence: Voluntary cooperation of the patient in taking drugs or medicine as prescribed. This includes timing, dosage, and frequency.Internet: A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.PubMed: A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.Societies, Pharmaceutical: Societies whose membership is limited to pharmacists.Simbu virus: A species in the ORTHOBUNYAVIRUS genus of the family BUNYAVIRIDAE family. Previously a large group of serotypes, most are now considered separate species.Methylurea Compounds: Urea compounds which are substituted with one or more methyl groups.Silk: A continuous protein fiber consisting primarily of FIBROINS. It is synthesized by a variety of INSECTS and ARACHNIDS.Spiders: Arthropods of the class ARACHNIDA, order Araneae. Except for mites and ticks, spiders constitute the largest order of arachnids, with approximately 37,000 species having been described. The majority of spiders are harmless, although some species can be regarded as moderately harmful since their bites can lead to quite severe local symptoms. (From Barnes, Invertebrate Zoology, 5th ed, p508; Smith, Insects and Other Arthropods of Medical Importance, 1973, pp424-430)Atrazine: A selective triazine herbicide. Inhalation hazard is low and there are no apparent skin manifestations or other toxicity in humans. Acutely poisoned sheep and cattle may show muscular spasms, fasciculations, stiff gait, increased respiratory rates, adrenal degeneration, and congestion of the lungs, liver, and kidneys. (From The Merck Index, 11th ed)Herbicides: Pesticides used to destroy unwanted vegetation, especially various types of weeds, grasses (POACEAE), and woody plants. Some plants develop HERBICIDE RESISTANCE.Simazine: A triazine herbicide.Triazines: Heterocyclic rings containing three nitrogen atoms, commonly in 1,2,4 or 1,3,5 or 2,4,6 formats. Some are used as HERBICIDES.Herbicide Resistance: Diminished or failed response of PLANTS to HERBICIDES.Hepatitis, Infectious Canine: A contagious disease caused by canine adenovirus (ADENOVIRUSES, CANINE) infecting the LIVER, the EYE, the KIDNEY, and other organs in dogs, other canids, and bears. Symptoms include FEVER; EDEMA; VOMITING; and DIARRHEA.Pesticides: Chemicals used to destroy pests of any sort. The concept includes fungicides (FUNGICIDES, INDUSTRIAL); INSECTICIDES; RODENTICIDES; etc.Methylmalonyl-CoA Decarboxylase: A carboxy-lyase that catalyzes the decarboxylation of (S)-2-Methyl-3-oxopropanoyl-CoA to propanoyl-CoA. In microorganisms the reaction can be coupled to the vectorial transport of SODIUM ions across the cytoplasmic membrane.Carbon-Carbon Ligases: Enzymes that catalyze the joining of two molecules by the formation of a carbon-carbon bond. These are the carboxylating enzymes and are mostly biotinyl-proteins. EC 6.4.Pyruvate Carboxylase: A biotin-dependent enzyme belonging to the ligase family that catalyzes the addition of CARBON DIOXIDE to pyruvate. It is occurs in both plants and animals. Deficiency of this enzyme causes severe psychomotor retardation and ACIDOSIS, LACTIC in infants. EC 6.4.1.1.Biotin: A water-soluble, enzyme co-factor present in minute amounts in every living cell. It occurs mainly bound to proteins or polypeptides and is abundant in liver, kidney, pancreas, yeast, and milk.Acetyl-CoA Carboxylase: A carboxylating enzyme that catalyzes the conversion of ATP, acetyl-CoA, and HCO3- to ADP, orthophosphate, and malonyl-CoA. It is a biotinyl-protein that also catalyzes transcarboxylation. The plant enzyme also carboxylates propanoyl-CoA and butanoyl-CoA (From Enzyme Nomenclature, 1992) EC 6.4.1.2.Biotinidase: An enzyme which catalyzes the release of BIOTIN from biocytin. In human, defects in the enzyme are the cause of the organic acidemia MULTIPLE CARBOXYLASE DEFICIENCY or BIOTINIDASE DEFICIENCY.Gene Expression Profiling: The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.Oligonucleotide Array Sequence Analysis: Hybridization of a nucleic acid sample to a very large set of OLIGONUCLEOTIDE PROBES, which have been attached individually in columns and rows to a solid support, to determine a BASE SEQUENCE, or to detect variations in a gene sequence, GENE EXPRESSION, or for GENE MAPPING.Aortopulmonary Septal Defect: A developmental abnormality in which the spiral (aortopulmonary) septum failed to completely divide the TRUNCUS ARTERIOSUS into ASCENDING AORTA and PULMONARY ARTERY. This abnormal communication between the two major vessels usually lies above their respective valves (AORTIC VALVE; PULMONARY VALVE).Gene Expression Regulation: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.Gene Expression: The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.Genomics: The systematic study of the complete DNA sequences (GENOME) of organisms.Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Desulfovibrio vulgaris: A species of gram-negative, anaerobic, rod-shaped bacteria isolated from soil, animal intestines and feces, and fresh and salt water.Desulfovibrio: A genus of gram-negative, anaerobic, rod-shaped bacteria capable of reducing sulfur compounds to hydrogen sulfide. Organisms are isolated from anaerobic mud of fresh and salt water, animal intestines, manure, and feces.Staphylococcaceae: Family of gram-positive, facultatively anaerobic bacteria, in the order Bacillales. Genera include Gemella, Macrococcus, Salinicoccus, and STAPHYLOCOCCUS.Computational Biology: A field of biology concerned with the development of techniques for the collection and manipulation of biological data, and the use of such data to make biological discoveries or predictions. This field encompasses all computational methods and theories for solving biological problems including manipulation of models and datasets.Amino Acid Motifs: Commonly observed structural components of proteins formed by simple combinations of adjacent secondary structures. A commonly observed structure may be composed of a CONSERVED SEQUENCE which can be represented by a CONSENSUS SEQUENCE.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Algorithms: A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.Terminology as Topic: The terms, expressions, designations, or symbols used in a particular science, discipline, or specialized subject area.Enzymes: Biological molecules that possess catalytic activity. They may occur naturally or be synthetically created. Enzymes are usually proteins, however CATALYTIC RNA and CATALYTIC DNA molecules have also been identified.International Agencies: International organizations which provide health-related or other cooperative services.Molecular Biology: A discipline concerned with studying biological phenomena in terms of the chemical and physical interactions of molecules.Databases, Genetic: Databases devoted to knowledge about specific genes and gene products.International Cooperation: The interaction of persons or groups of persons representing various nations in the pursuit of a common goal or interest.

Purification and characterization of allophanate hydrolase (AtzF) from Pseudomonas sp. strain ADP. (1/14)

AtzF, allophanate hydrolase, is a recently discovered member of the amidase signature family that catalyzes the terminal reaction during metabolism of s-triazine ring compounds by bacteria. In the present study, the atzF gene from Pseudomonas sp. strain ADP was cloned and expressed as a His-tagged protein, and the protein was purified and characterized. AtzF had a deduced subunit molecular mass of 66,223, based on the gene sequence, and an estimated holoenzyme molecular mass of 260,000. The active protein did not contain detectable metals or organic cofactors. Purified AtzF hydrolyzed allophanate with a k(cat)/K(m) of 1.1 x 10(4) s(-1) M(-1), and 2 mol of ammonia was released per mol allophanate. The substrate range of AtzF was very narrow. Urea, biuret, hydroxyurea, methylcarbamate, and other structurally analogous compounds were not substrates for AtzF. Only malonamate, which strongly inhibited allophanate hydrolysis, was an alternative substrate, with a greatly reduced k(cat)/K(m) of 21 s(-1) M(-1). Data suggested that the AtzF catalytic cycle proceeds through a covalent substrate-enzyme intermediate. AtzF reacts with malonamate and hydroxylamine to generate malonohydroxamate, potentially derived from hydroxylamine capture of an enzyme-tethered acyl group. Three putative catalytically important residues, one lysine and two serines, were altered by site-directed mutagenesis, each with complete loss of enzyme activity. The identity of a putative serine nucleophile was probed using phenyl phosphorodiamidate that was shown to be a time-dependent inhibitor of AtzF. Inhibition was due to phosphoroamidation of Ser189 as shown by liquid chromatography/matrix-assisted laser desorption ionization mass spectrometry. The modified residue corresponds in sequence alignments to the nucleophilic serine previously identified in other members of the amidase signature family. Thus, AtzF affects the cleavage of three carbon-to-nitrogen bonds via a mechanism similar to that of enzymes catalyzing single-amide-bond cleavage reactions. AtzF orthologs appear to be widespread among bacteria.  (+info)

Allophanate hydrolase, not urease, functions in bacterial cyanuric acid metabolism. (2/14)

Growth substrates containing an s-triazine ring are typically metabolized by bacteria to liberate 3 mol of ammonia via the intermediate cyanuric acid. Over a 25-year period, a number of original research papers and reviews have stated that cyanuric acid is metabolized in two steps to the 2-nitrogen intermediate urea. In the present study, allophanate, not urea, was shown to be the 2-nitrogen intermediate in cyanuric acid metabolism in all the bacteria examined. Six different experimental results supported this conclusion: (i) synthetic allophanate was shown to readily decarboxylate to form urea under acidic extraction and chromatography conditions used in previous studies; (ii) alkaline extraction methods were used to stabilize and detect allophanate in bacteria actively metabolizing cyanuric acid; (iii) the kinetic course of allophanate formation and disappearance was consistent with its being an intermediate in cyanuric acid metabolism, and no urea was observed in those experiments; (iv) protein extracts from cells grown on cyanuric acid contained allophanate hydrolase activity; (v) genes encoding the enzymes AtzE and AtzF, which produce and hydrolyze allophanate, respectively, were found in several cyanuric acid-metabolizing bacteria; and (vi) TrzF, an AtzF homolog found in Enterobacter cloacae strain 99, was cloned, expressed in Escherichia coli, and shown to have allophanate hydrolase activity. In addition, we have observed that there are a large number of genes homologous to atzF and trzF distributed in phylogenetically distinct bacteria. In total, the data indicate that s-triazine metabolism in a broad class of bacteria proceeds through allophanate via allophanate hydrolase, rather than through urea using urease.  (+info)

Purification and characterization of TrzF: biuret hydrolysis by allophanate hydrolase supports growth. (3/14)

TrzF, the allophanate hydrolase from Enterobacter cloacae strain 99, was cloned, overexpressed in the presence of a chaperone protein, and purified to homogeneity. Native TrzF had a subunit molecular weight of 65,401 and a subunit stoichiometry of alpha(2) and did not contain significant levels of metals. TrzF showed time-dependent inhibition by phenyl phosphorodiamidate and is a member of the amidase signature protein family. TrzF was highly active in the hydrolysis of allophanate but was not active with urea, despite having been previously considered a urea amidolyase. TrzF showed lower activity with malonamate, malonamide, and biuret. The allophanate hydrolase from Pseudomonas sp. strain ADP, AtzF, was also shown to hydrolyze biuret slowly. Since biuret and allophanate are consecutive metabolites in cyanuric acid metabolism, the low level of biuret hydrolase activity can have physiological significance. A recombinant Escherichia coli strain containing atzD, encoding cyanuric acid hydrolase that produces biuret, and atzF grew slowly on cyanuric acid as a source of nitrogen. The amount of growth produced was consistent with the liberation of 3 mol of ammonia from cyanuric acid. In vitro, TrzF was shown to hydrolyze biuret to liberate 3 mol of ammonia. The biuret hydrolyzing activity of TrzF might also be physiologically relevant in native strains. E. cloacae strain 99 grows on cyanuric acid with a significant accumulation of biuret.  (+info)

The structure of allophanate hydrolase from Granulibacter bethesdensis provides insights into substrate specificity in the amidase signature family. (4/14)

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Structure and function of allophanate hydrolase. (5/14)

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Control of vacuole permeability and protein degradation by the cell cycle arrest signal in Saccharomyces cerevisiae. (6/14)

Saccharomyces cerevisiae responds to deperivation of nutrients by arresting cell division at the unbudded G1 stage. Cells situated outside of G1 at the time of deperivation complete the cell cycle before arresting. This prompted an investigation of the source of nutrients used by these cells to complete division and the mechanisms controlling their availability. We found a close correlation between accumulation of unbudded cells and loss of previously formed allophanate hydrolase activity after nutrient starvation. These losses were not specific to the allantoin, system since they have been observed for a number of other enzymes and also when cellular protein levels were monitored with [3H]leucine. Loss of hydrolase activity was also observed when protein synthesis was inhibited either by addition of inhibitors or loss of the prtl gene product. We found that onset of nutrient starvation brought about release of large quantities of arginine and allantoin normally sequestered in the cell vacuole. Treatment of a cells with alpha-factor resulted in both the release of allantoin and arginine from the cell vacuole and the onset of intracellular protein degradation. These effects were not observed when either alpha cells or a/alpha diploid strains were treated with alpha-factor. These data suggest that release of vacuolar constitutents and protein turnover may be regulated by the G1 arrest signal.  (+info)

Structural analysis of the dur loci in S. cerevisiae: two domains of a single multifunctional gene. (7/14)

In Saccharomyces cerevisiae, the degradation of urea to carbon dioxide and ammonia is catalyzed by urea carboxylase and allophanate hydrolase. The loci coding for these enzymes (dur1 and dur2) are very tightly linked on the right arm of chromosome II between pet11 and met8. Pleiotropic mutations that fail to complement mutations in either of the dur loci were found to be predominantly located in or near the dur2 locus. We interpret these data as suggesting that the two dur loci might in reality be domains of a single gene that codes for a multifunctional polypeptide. In view of this conclusion, we have renamed the dur loci as the dur1,2 locus.  (+info)

Urea carboxylase and allophanate hydrolase are components of a multifunctional protein in yeast. (8/14)

Saccharomyces cerevisiae can use urea as sole nitrogen source by degrading it in two steps (urea carboxylase and allophanate hydrolase) to ammonia and carbon dioxide. We previously demonstrated that: 1) the enzymatic functions required for degradation are encoded in two tightly linked genetic loci and 2) pleiotropic mutations each resulting in the loss of both activities are found in both loci. These and other observations led to the hypothesis that urea degradation might be catalyzed by a multifunctional polypeptide. Waheed and Castric (1977) J. Biol. Chem. 252, 1628-1632), on the other hand, purified urea amidolyase from Candida utilis and reported it to be a tetramer composed of nonidentical 70- and 170-kilodalton subunits. To resolve the differing views of urea amidolyase structure, we purified the protein using rapid methods designed to avoid proteolytic cleavage. Application of these methods resulted in the isolation of a single, inducible and repressible, 204-kilodalton species. We observed no evidence for the existence of nonidentical subunits. A similar inducible, high molecular weight species was also detected in C. utilis. These biochemical results support our earlier hypothesis that urea degradation is carried out in yeast by an inducible and repressible protein composed of identical, multifunctional subunits.  (+info)

Urea Amidolyase; Contains Both Urea Carboxylase And Allophanate Hydrolase Activities, Degrades Urea To CO2 And NH3; Expression Sensitive To Nitrogen Catabolite Repression And Induced By Allophanate, An Intermediate In Allantoin Degradation; Protein Abundance Increases In Response To DNA Replication Stress
Where will your ride take you today? When you dont really know, Roam Disc is a perfect choice. Its built for any terrain, so your choices are unlimited. Smooth roads, rough roads, dirt paths or trails, Roam Disc keeps you rolling in comfort and style.. With its confident, flat handlebar positioning and lightweight ALUXX aluminum frame, Roam Disc blends all the best qualities of a mountain bike and a road bike. Its front suspension adds comfort and control. It has powerful disc brakes and durable components, so it can take some bumps on rough city roads. And the fast rolling 700c wheels can handle smooth roads or dirt, making it a great choice for town-to-trail adventures ...
am1a04d AJ229366 409 Homolog to proteins of other species than S. cerevisiae (see table 2) am1a07d AJ229367 307 am1a12d AJ229368 234 am1b02d AJ229369 236 am1b03d AJ229370 123 am1b04d AJ229371 314 7.0 E-41 YFL036w RPO41 DNA-directed RNA pol., mitochondrial am1b06d AJ229372 410 4.7 E-41 YGR208w SER2 phosphoserine phosphatase am1b11d AJ229373 327 2.1 E-42 YDL213c RNA recognition domain in the N-terminal region am1b12d AJ229374 280 am1c01d AJ229375 489 3.0 E-100 YMR229c RRP5 processing of pre-ribosomal RNA am1c05d AJ229376 275 am1c08d AJ229377 288 1.5 E-56 YDL102w CDC2 DNA-directed DNA pol. delta, catalytic 125 KD subunit am1c11d AJ229378 157 am1c12d AJ229379 145 am1d02d AJ229380 322 am1d05d AJ229381 195 repeats or mito am1d06d AJ229382 302 5.1 E-55 YBR208c DUR1,2 urea amidolyase am1d08d AJ229383 241 am1d12d AJ229384 174 am1e05r AJ229385 220 6.6 E-15 YMR287c MSU1 3-5 exonuclease for RNA 3 ss-tail, mitochondrial am1e06d AJ229386 290 am1e09r AJ229387 241 am1e10r AJ229388 138 1.7 E-06 YDL153c SAS10 ...
Formerly Stanley InnerSpace 4700SC. Roam closed suture cart is a lightweight, durable, medical supply cart featuring locking doors and 10 adjustable shelves . Each shelf holds 9 suture boxes.
[tag-box] The Key to Elephants Future? Room to Roam. Dr. Max Graham, the CEO of Space for Giants-our Lip Veil and Luminescent Eye Shade philanthropy partner-has crafted a strategy for saving elephants while also protecting the people who live near them. [social-share] If you could conjure someone from central casting
Looking for STANLEY ROAMALERT Wrist Strap, 7 Length, 13/64 Height, For Use With Roam Alert System, Gray, PK 5 (462C53)? Graingers got your back. Price $222.00. Easy online ordering for the ones who get it done along with 24/7 customer service, free technical support & more.
Roam specialty medical supply carts are mobile, maximize space and help increase efficiency. Choose from open or closed suture carts or scope carts.
The zombies in World War Z are just actors - but in the animal world, zombies are a fact of life. Evolution can come up with some fiendish twists: For example, there are some species that bend other creatures to their will to keep the
We are super excited to be spending time outdoors this summer in our newly updated rooftop garden. Julie deLeon of Groundwork Design brought this beauty to life literally with her gorgeous live roof & container plantings. It started with our friend Joe Lambke, planning out the initial hardscaping, then with Jacob Shapiro & team from…
MyNorth.com is the online home of Traverse, Northern Michigans Magazine, the flagship publication of MyNorth Media, a Traverse City, Michigan company dedicated to sharing stories and photos about vacations, restaurants, wineries, the outdoors and more from Traverse City to Sleeping Bear Dunes and up to Mackinac Island.. ...
Feed Type Link http://www.venturedeal.com/Search/SearchResultTransactionDetail.aspx?TransactionId=e60cdb7c-c6c0-44eb-82c8-0e5a041ebb01 Date 5/4/2016
Mutants (Sub 2 Mutation) - c64 remix by vurtX. Original by Fred Gray. Released 24/03/2012. Hi, this took me forever to make because I was wasnt very happy with it for a long time, but it isnt going to be getting much better so its time to let it roam free on the internet, the original SiD also reminds me of Bjorks Army of Me so I put a li...
Dev is a seasoned, creative and driven Product leader. His desire to build products for social impact brought him to Dimagi. Dev comes to Dimagi after building successful tools/products in the collaboration, content management and mobile payments space. Prior to joining Dimagi, Dev drove growth at ROAM (acquired by Ingenico), where he led their Mobile…
Animal experts tell the Chamwada Report that most dogs roam the streets as a result of negligence from their owners. And this comes with risks as stray dogs often attack people not known to them...
Sign up to Roam, Run or Ride in your Red Socks and raise vital funds to support people living with kidney disease. Register today.
English , Publisher: Audio CD (October 1, 2011) , ISBN-10: 1599508001 , MP3 192Kbps , 376.71 MB. The blighted aftermath of a global nuclear showdown, Deathlands exacts a blood price. The living pay it; the dead dont care. For one legendary band of warriors, this barbaric new world holds a chance for redemption: the secrets of the past. They roam a disfigured America, searching for pre-dark tech.... ...
He does not sleep much in his crib. It has been like that since he knows how to turn over. So we put him to sleep with us on the bed. Now during weekend when only me and otger kids around, he will be confined in a wooden crib. As long someone to play or talk to him, he would be fine in there. Otherwise if we let him loose, he will roam around or hurt himself. And I can go on do my chore or have a peaceful toilet moment. *wink ...
Theres this field in which we like to play. Its isolated enough that I feel comfortable letting all three of my little folks roam. Its hard to find much open space around here, still in city limits, thats far enough away from traffic and other animals and construction and trains. ...
There are many sexually-transmitted diseases that roam the earth today. They are known to trigger various harm and damage to anybody that they strike. Because of that, everyone is warned to be careful with everything that they do.
IT’S that time of the year again when the flu roams the streets of Glasgow and East Renfrewshire searching for fresh victims.
Weve met the 7 brothers, lets see what theyve been up to the first week. Remember, each Sim was actively played only one day, the other six days he could roam around freely. Primus ✓ finished 3 paintings ✓ painted for 5 hours - went for a jog - beat up his enemy - napped…
A good friend came to see me this evening. The nurses let me roam free after sticking this thing in my arm and we went for a CrappoBurger over the road. Company is appreciated. Spend too long alone and you just cant but think about things like spleens ...
Stefan Grouter is completing a PhD down at RMIT working on procedural 3D models for virtual worlds. Looks interesting, and a possible liason partner in the future if this temporal ROAM stuff ever gets underway. After months of procrastination Ive finished the proposal, only to find that Ron is snowed under and unable to take on further students. Looks like theres some interesting oportunities afield though ...
Charles Back, the owner of Fairview, is also the man behind Goats do Roam, the fruit for which is sourced primarily from Paarl, Malmesbury and Paardeberg. The newly packaged Goats in Villages Viogner is now 100% Viognier with 30% barrel fermented fr...
The experts at Travel Channels Roam blog share tips on how to fly like a pro with airport hacks, in-flight beauty tricks and tips for sleeping on a plane.
Join ROAM for 13 days of multi-sport in Peru Sacred Valley. Trek lodge-to-lodge on Salkantay Inca Trail to Machu Picchu, see Maras & Moray and much more!
Warning! Dinosaurs have invaded the MIT lab! They may one day "roam" through a museum near you, too.. Researchers at the Massachusetts Institute of Technology Artificial Intelligence (AI) Lab in Cambridge have created a robotic dinosaur. The lab hopes to develop small, lifelike 3D representations of dinosaurs that will be used as educational tools. The project falls under the administration of MITs Leg Lab, a division of the AI Lab dedicated to simulating and building robots that walk like their biological counterparts.. After five years of work, scientist Peter Dilworth has created a robotic model of a troodon dinosaur (see the figure). The robotic version of this creature, dubbed Troody, can walk across a desk with lifelike body movement.. Currently, Troody is one of two bipedal robots at the Leg Lab. Known as M2, the other biped simulates the legs of a human. While it can stand and walk in place, M2 cannot yet walk forward. Series elastic actuators, or springs connected to motors, are ...
How do you determine the sex of a teletubby anyway?. Teletubbies are one of the few, rare species to carry their gonads in a detachable, external sack. Therefore we know Tinky is a male because of his "purse".. This condition is only temporary, though, as Teletubbies also share their sex communaly. The "purse" belongs to each member of the clan and can be traded between members in exchange for certain favors. The troupe as a whole can be thought of as "male", though, as there are in fact no female Teletubbies, it is a distinction of trivial importance. It would be more proper to refer to Tinky as the (currently) "sexed" Teletubby, rather than male or female.. Reproduction only occurs by accident, and at this point in time, neither Teletubbies nor researchers have been able to determine its cause. Therefore, while it is safe to say that Teletubbies are homoscial they are not, by definition, homosexual.. Except for Po. Dirty, dirty Po ...
Travel down any given highway in Alberta on any weekend and youre sure to see tourists pressed up against the inside of their car windows, hoping to catch a glimpse of the provinces wildlife. Often,...
The number of patients at Brooklyns Long Island College Hospital has dwindled to 18 amid a legal stalemate between its owner, which wants to close it, and unions representing workers.
Every US president has walked the storied streets of one particular city in Virginia. On this fun-filled scavenger hunt of Alexandria, journey around Old Towns historic buildings, great green spaces and cultural institutions. Where does George Washington like to hang out? How do you get invited to a lyceum? Find out on this scavenger hunt of Alexandria! This scavenger hunt is app-lead. No tour guide or reservation required.
A couple of years ago, some 11 year old boy killed the largest wild boar anyone has ever seen. The thing is unbelievably massive. Actually, to be exact, it was "1,051 pounds and measured 9-feet-4 from the tip of its snout to the base of its tail". Seriously? No one notice this thing walking around? Its bigger than my car. And he killed it with a hand gun? Who goes hunting with a hand gun? After reading about this pig-monster, I could think about only 1 thing: how sick of eating pork this kids family is going to become. Some more pictures of such giant animals after the jump ...
Minneopa State Parks expansive prairie could become home to a bison herd if approved by the Department of Natural Resources following a public comment period.
Heartbreaking footage of a starving polar bear desperately searching for food on iceless land highlights the “real face of climate change”, conservationists say.
Tesla, the obnoxious green car company whose products roam the streets of Beverly Hills and carry its assorted movie stars, is about to get a lesson in sustainability if the Republican congressional tax reform bill goes through. No more free stuff. Here´s one example; Will.i.am´s custom wide-body Tesla S parked on Rodeo Drive in Beverly Hills. Highsnobiety has the details. Here´s another: Mel Gibson having a cow over a valet not bringing him back his Tesla soon enough. Daily Mail on the job. Turns out the big reason anyone buys one of these green vehicles isn´t to virtue-signal, although there´s a lot ...
Why are rabbits not allowed to roam freely in nature? Why do we use them to perform experiments? Rabbits are frequent victims of animal experimenters because they are mild-tempered and easy to handle, confine, and breed-more than 241,000 of them are abused in U.S. laboratories every year. ...
Jim White - Burn The River Dry Lyrics. Door is locked, no ones home Frame is empty, pictures missing Throw that rock right through the window Hey, I know him, hes a singer Roam
My family and I absolutely love dogs! We have 3 of our own, and they are our world. We have a large house and the dogs have freedom to roam and pla...
There are many stories about the hotel and what has occurred there. All of these are written down in the guest book which of course we read through! There are stories of strange noises coming from empty rooms; coughing, labored breathing, and even voices. Sometimes these noises come from a dark corner of a guests room! Others report lights or TVs turning on and off. There are unexplained smells of flowers, dust, cigarettes and whiskey. Ghostly presences and unexplained activities lure many to this haunted hotel. Two female apparitions have been seen by numerous visitors. The first, a nurse carrying a clipboard, seems more to be a memory than an actual ghost. She roams the halls, pausing at intervals and leaning down, looking at beds that no longer exist and patients long since dead. The other, a woman in white, has been identified as a woman who died in childbirth. Neither she nor her baby left the hospital halls. According to legend she is distraught over the treatment of her dead child, who ...
Ive made a reader survey to answer some burning questions I have for you - like your age group and which country you live in. And also does my Read More doohickey annoy you? Do you click on it to read the whole post, or would you like me to post everythaaaang there so your eyes can roam freely? What kind of posts you want to see more of from me, and what are your fave kind of posts to read? I value your opinion and its all totally anonymous ...
So far in my searches, Ive only found LARGE types of housings and feeders; but I will never keep over 3 mature hogs at a time. (I only have 6 acres total of land they can roam in and my family is small and I do not plan on selling any.)
A 50 lb bag of feed usually lasts me about 6-8 days served dry. I would long to be able to feed my chickens organic, non-GMO pellet but it just wasnt in the budget. They roam all day so theyre getting as much organic matter as they want, but I still longed for better pellets. […]. ...
Nuclear Throne is a post-apocalyptic roguelike-like top-down shooter. Not the final hope of humanity post-apocalyptic, but humanity is extinct and mutants and monsters now roam the world post-apocalyptic. Fight your way through the wastelands with powerful weaponry, collecting radiation to mutate some new limbs and abilities.
The Act enumerates different variants of cruelty to animals under Section 11 as the following actions:. a) Beating, kicking, overriding, overloading, torturing and causing unnecessary pain to any animal.. b) Using an old or injured or unfit animal for work (the punishment applies to the owner as well as the user).. c) Administering an injurious drug/medicine to any animal.. d) Carrying an animal in any vehicle in a way that causes it pain and discomfort.. e) Keeping any animal in a cage where it doesnt have reasonable opportunity of movement.. f) Keeping an animal on an unreasonably heavy or short chain for an unreasonable period of time.. g) Keeping an animal in total and habitual confinement with no reasonable opportunity to exercise.. h) Being an owner failing to provide the animal with sufficient food, drink or shelter.. i) Abandoning an animal without reasonable cause.. j) Willfully permitting an owned animal to roam on streets or leaving it on the streets to die of disease, old age or ...
Naruto woke up the next Tuesday morning to the sound of the horses wanting out. He hopped down from the loft and let them out into the field. He watched them roam around for about an hour before he heard some commotion coming from the town. He hurried to see what was going on, and in the main street was a parade of carriages and royal soldiers. Naruto couldnt see clearly so he climbed a single tree right next to the road and leaned against one of the branches. In the biggest open carriage he saw the king, who he recognized easily because every once in a while he would come to check on the horses. It was the one sitting next to the king who caught Narutos eye, a beautiful young girl with long blue hair. She sat silently at the kings side with an innocent face, looking around at the villagers.. "Wow…" Naruto gazed at her, he was so mesmerized he was not paying attention to what the king was saying.. "My people, I am pleased to announce that finally our country will forge an alliance with our ...
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Rad Resource: Looking for inspiration? Check out the RSA Animate videos, which are high-quality visualizations of visionary talks put on by the RSA.. Hot Tip: You dont need an art background to practice visual recording. I have some basic knowledge of composition and shapes from a couple of drawing and photography courses I took in college-quite some time ago. Really, I just started drawing in a staff meeting one day, and thus a graphic recorder was born. Graphic recorders often work off of templates to give a shape to a visualization, and the point is to populate a graphic recording with easy-to-recognize images. They do not have to be works of fine art.. Rad Resource: Wondering how to get started? You can browse Dan Roams Napkin Academy, or practice by incorporating graphic recording methods into your own note taking.. Do you have questions, concerns, kudos, or content to extend this aea365 contribution? Please add them in the comments section for this post on the aea365 webpage so that we ...
My pump is always attached right at my left hip. I hardly ever place it anywhere else except at night where it roams free! Cherry ----- Original Message ----- From: Sue Kinzelman ,email @ redacted, To: ,email @ redacted, Sent: Tuesday, August 28, 2001 8:30 AM Subject: Re: [IP] insulin , It would be interesting to find out, of the folks who have no problems , with their insulin losing its effectiveness, where do they wear the , pump? Is it right next to the body or attached somewhere cooler. , , Sue ---------------------------------------------------------- for HELP or to subscribe/unsubscribe, contact: [email protected] send a DONATION http://www.Insulin-Pumpers.org/donate.shtml ...
This is a pretty impressive bulldog! Lets hope he doesnt come alive after midnight and roam the neighborhood. The Citadel Bulldog is the logo for the Citadel Bulldog Athletic Teams. Private First Class Davis offered to pose next to him to show the scale ...
I suppose this pre 4th of July post should have really been done in red, white and blue, but I just didnt think ahead. Oh well, I hope you have a festive holiday anyway. Im looking forward to three days off, a little trip away from home and some time for quiet contemplation. I did all the end of the week chores earlier this week so I am free to roam around this weekend. Im hoping to get some really good pictures to post on my new photography blog while Im away. For the shots I want to capture I really need a specific filter that I dont have, but Im going to see how well I can do without it. Wish me luck. Hey, nothing ventured nothing gained, right? Why dont we take a look at whats blooming in the garden ...
Allophanate hydrolase Roon RJ; Levenberg B (1970). "ATP-Urea amidolyase (ADP) (Candida utilis)". Methods Enzymol. 17A: 317-324 ... Sumrada RA, Cooper TG (1982). "Urea carboxylase and allophanate hydrolase are components of a multifunctional protein in yeast ... allophanate). This enzyme belongs to the family of ligases, specifically those forming generic carbon-nitrogen bonds. The ...
Urea carboxylase Allophanate hydrolase Urease test Holm L, Sander C (1997). "An evolutionary treasure: unification of a broad ...
... allophanate hydrolase MeSH D08.811.277.087.100 --- arylformamidase MeSH D08.811.277.087.116 --- asparaginase MeSH D08.811. ... thiolester hydrolases MeSH D08.811.277.352.897.075 --- acetyl-CoA hydrolase MeSH D08.811.277.352.897.700 --- palmitoyl-coa ... phosphoric diester hydrolases MeSH D08.811.277.352.640.050 --- annexin A3 MeSH D08.811.277.352.640.125 --- 3',5'-cyclic-GMP ... pyroglutamate hydrolase MeSH D08.811.277.087.831 --- sirtuins MeSH D08.811.277.087.902 --- urease MeSH D08.811.277.151.300 --- ...
In enzymology, an allophanate hydrolase (EC 3.5.1.54) is an enzyme that catalyzes the chemical reaction allophanate + 3 H2O + ... Kanamori T, Kanou N, Kusakabe S, Atomi H, Imanaka T (2005). "Allophanate hydrolase of Oleomonas sagaranensis involved in an ATP ... Sumrada RA, Cooper TG (1982). "Urea carboxylase and allophanate hydrolase are components of a multifunctional protein in yeast ... Urea carboxylase Maitz GS, Haas EM & Castric PA (1982). "Purification and properties of the allophanate hydrolase from ...
... allophanate hydrolase EC 3.5.1.55: long-chain-fatty-acyl-glutamate deacylase EC 3.5.1.56: N,N-dimethylformamidase EC 3.5.1.57: ... phloretin hydrolase EC 3.7.1.5: acylpyruvate hydrolase EC 3.7.1.6: acetylpyruvate hydrolase EC 3.7.1.7: beta-diketone hydrolase ... bile-acid-CoA hydrolase EC 3.1.2.27: choloyl-CoA hydrolase EC 3.1.2.28: 1,4-dihydroxy-2-naphthoyl-CoA hydrolase EC 3.1.2.29: ... acetyl-CoA hydrolase EC 3.1.2.2: palmitoyl-CoA hydrolase EC 3.1.2.3: succinyl-CoA hydrolase EC 3.1.2.4: 3-hydroxyisobutyryl-CoA ...
Expression Sensitive To Nitrogen Catabolite Repression And Induced By Allophanate, An Intermediate In Allantoin Degradation; ... Contains Both Urea Carboxylase And Allophanate Hydrolase Activities, Degrades Urea To CO2 And NH3; ... Urea amidolyase; contains both urea carboxylase and allophanate hydrolase activities, degrades urea to CO2 and NH3; expression ... DUR80, bifunctional urea carboxylase/allophanate hydrolase, L000000532, L000000533, L000000536, YBR208C. ...
In enzymology, an allophanate hydrolase (EC 3.5.1.54) is an enzyme that catalyzes the chemical reaction allophanate + 3 H2O + ... Kanamori T, Kanou N, Kusakabe S, Atomi H, Imanaka T (2005). "Allophanate hydrolase of Oleomonas sagaranensis involved in an ATP ... Sumrada RA, Cooper TG (1982). "Urea carboxylase and allophanate hydrolase are components of a multifunctional protein in yeast ... Urea carboxylase Maitz GS, Haas EM & Castric PA (1982). "Purification and properties of the allophanate hydrolase from ...
Allophanate hydrolase Roon RJ; Levenberg B (1970). "ATP-Urea amidolyase (ADP) (Candida utilis)". Methods Enzymol. 17A: 317-324 ... Sumrada RA, Cooper TG (1982). "Urea carboxylase and allophanate hydrolase are components of a multifunctional protein in yeast ... allophanate). This enzyme belongs to the family of ligases, specifically those forming generic carbon-nitrogen bonds. The ...
3.5.1.54 allophanate hydrolase 3.5.1.55 long-chain-fatty-acyl-glutamate deacylase ... 3.5.1.101 L-proline amide hydrolase 3.5.1.102 2-amino-5-formylamino-6-ribosylaminopyrimidin-4(3H)-one 5-monophosphate ...
... and allophanate hydrolase (Nham_2040; COG2049). The latter two enzymes putatively contribute to ATP-dependent urea amidolyase ... All NOB genomes lack genes encoding a classical urease (ATP-independent urea hydrolase) or a urea transporter identified ... hydrolases, and sialic-acid based homopolysaccharide formation genes, some of which have little sequence similarity to any ...
This pathway involves urea carboxylase and allophanate hydrolase activity thought to be also involved in urea degradation ( ... Kanamori, T., Kanou, N., Kusakabe, S., Atomi, H., and Imanaka, T. (2005). Allophanate hydrolase of Oleomonas sagaranensis ...
cyn:Cyan7425_3252 allophanate hydrolase K01457 607 100 ( -) 29 0.300 150 -, 1 fjg:BB050_03756 2-oxoisovalerate dehydrogenase ... ase:ACPL_5595 alpha/beta hydrolase fold protein 255 109 ( 7) 31 0.330 103 -, 2 asf:SFBM_0853 3-oxoacyl-[acyl-carrier-protein] ... taa:NMY3_01686 Alpha/beta hydrolase family protein K06889 346 104 ( -) 30 0.324 71 -, 1 tne:Tneu_1901 Radical SAM domain ...
ror:RORB6_06485 allophanate hydrolase K01457 599 102 ( -) 29 0.301 73 -, 1 rro:104674625 early B-cell factor 4 K09103 645 102 ... sphk:SKP52_11960 glycosyl hydrolase family protein K05349 877 105 ( -) 30 0.307 189 ,-, 1 syc:syc0479_d oxidoreductase aldo/ ... scb:SCAB_25521 putative glycosyl hydrolase K01811 683 100 ( -) 29 0.316 79 -, 1 smt:Smal_3299 phosphoesterase PA-phosphatase ... rmu:RMDY18_03680 Zn-dependent hydrolase including glyox 322 112 ( -) 31 0.320 103 -, 1 rpe:RPE_0024 gamma-glutamyltransferase ...
Cheng G, Shapir N, Sadowsky MJ, Wackett LP (2005) Allophanate hydrolase, not urease, functions in bacterial cyanuric acid ...
Urea amidolyase catalyzes the two reactions (urea carboxylase and a allophanate hydrolase) associated with urea degradation in ... These data are consistent with the suggestion that sequential induction of allophanate hydrolase and urea carboxylase ... we determined whether or not a detectable period of time elapsed between the appearance of allophanate hydrolase activity and ... approximately 2 to 6 min elapsed between the appearance of allophanate hydrolase and urea carboxylase activities. In search of ...
... a KipI-KipA homolgue incorrectly annotated as an allophanate hydrolase. Acta Cryst D67:105-111Google Scholar ...
... contains both urea carboxylase and allophanate hydrolase activities. It also converts allantoin to ammonia and carbon dioxide, ...
... and allophanate hydrolase activities (29, 34). The predicted binding sites of pseudo-NikR regulators are present upstream of ...
... allophanate hydrolase pathway, as in many bacteria known to use urea as the sole nitrogen source (26) (Fig. 5). The uca gene ... In the absence of gmaS, direct oxidative deamination of glutamate by NMG synthase (hydrolase) may yield sufficient ammonium for ...
... allophanate) catalyzed by urea carboxylase, followed by hydrolysis to CO2 and ammonia by allophanate hydrolase (see Additional ... Two pathways seem to be present for the degradation of urea, namely, direct cleavage by urease (ureABC), and an allophanate ... The genome contains several genes coding for putative lipases/esterases of the α/β-hydrolase fold-superfamily or the SGNH- ... Urate degradation probably involves urate oxidase and 5-hydroxyisourate hydrolase. The next step, catalyzed by OHCU ...
Allophanate hydrolase (substance). Code System Preferred Concept Name. Allophanate hydrolase (substance). Concept Status. ...
The Structure of Allophanate Hydrolase from Granulibacter bethesdensis Provides Insights into Substrate Specificity in the ...
Allophanate hydrolase subunit 2. cog/ cog. urea_amlyse_rel. tigr/ tigrfam. Gene Info. Organism:. Bacillus subtilis. Locus tag: ... putative hydrolase subunit antagonist of KipI (RefSeq). 176, 240. BSU04100. kipR. transcriptional regulator (IclR family) ( ...
Isochorismatase hydrolase. Allophanate hydrolase. 0.914. Psed_3186. Psed_3185. Psed_3186. Psed_3185. Isochorismatase hydrolase ...
Allophanate hydrolase subunit 1. 0.565. Noca_0048. Noca_2978. GntR family transcriptional regulator ...
Allophanate hydrolase subunit 1. cog/ cog. peptidyl-prolyl cis-trans isomerase activity. go/ molecular_function. protein ... Predicted hydrolase of the metallo-beta-lactamase superfamily (NCBI). 84, 361. RSP_2599. phoB. Phosphate regulon ...
Allophanate hydrolase subunit 2 YP_003110896 normal 0.368046 normal 0.0250714 Catenulispora acidiphila DSM 44928 Bacteria -. ... HAD-superfamily hydrolase, subfamily IA, variant 1 YP_003110860 normal 1 normal 1 Catenulispora acidiphila DSM 44928 Bacteria - ... HAD-superfamily subfamily IB hydrolase, TIGR01490 YP_003110850 normal 1 normal 1 Catenulispora acidiphila DSM 44928 Bacteria - ...
Allophanate was subsequently cleaved by an allophanate hydrolase. ... but a more recent study showed that biuret hydrolysis in these bacteria produced allophanate . ...
... allophanate hydrolase; UREASE, urea carboxylase; ARGN, arginase) were predicted as top targets (Table 2). In the nitrogen- ...
In Allophanate hydrolase 2 and Biotin carboxylase cluster its role is Allophanate hydrolase 2 subunit 2 (EC 3.5.1.54). ... PGF_00044332: Allophanate hydrolase 2 subunit 2 (EC 3.5.1.54). PLF_1279_00000412: Allophanate hydrolase 2 subunit 2 (EC 3.5. ... In Allophanate hydrolase 2 and Biotin carboxylase cluster its role is prokaryotic 5-oxoprolinase C, pxpC. ... In COG0327 DUF34 its role is Allophanate hydrolase 2 subunit 2 (EC 3.5.1.54). However, the functionality of this subsystem has ...
  • In enzymology, an allophanate hydrolase (EC 3.5.1.54) is an enzyme that catalyzes the chemical reaction allophanate + 3 H2O + H+ ⇌ {\displaystyle \rightleftharpoons } 2 HCO3− + 2 NH4+ Thus, the two substrates of this enzyme are allophanate (urea-1-carboxylate or N-carbamoylcarbamate) and H2O, whereas its two products are HCO3− and NH4+. (wikipedia.org)
  • This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. (wikipedia.org)