Purification and characterization of allophanate hydrolase (AtzF) from Pseudomonas sp. strain ADP. (1/14)

AtzF, allophanate hydrolase, is a recently discovered member of the amidase signature family that catalyzes the terminal reaction during metabolism of s-triazine ring compounds by bacteria. In the present study, the atzF gene from Pseudomonas sp. strain ADP was cloned and expressed as a His-tagged protein, and the protein was purified and characterized. AtzF had a deduced subunit molecular mass of 66,223, based on the gene sequence, and an estimated holoenzyme molecular mass of 260,000. The active protein did not contain detectable metals or organic cofactors. Purified AtzF hydrolyzed allophanate with a k(cat)/K(m) of 1.1 x 10(4) s(-1) M(-1), and 2 mol of ammonia was released per mol allophanate. The substrate range of AtzF was very narrow. Urea, biuret, hydroxyurea, methylcarbamate, and other structurally analogous compounds were not substrates for AtzF. Only malonamate, which strongly inhibited allophanate hydrolysis, was an alternative substrate, with a greatly reduced k(cat)/K(m) of 21 s(-1) M(-1). Data suggested that the AtzF catalytic cycle proceeds through a covalent substrate-enzyme intermediate. AtzF reacts with malonamate and hydroxylamine to generate malonohydroxamate, potentially derived from hydroxylamine capture of an enzyme-tethered acyl group. Three putative catalytically important residues, one lysine and two serines, were altered by site-directed mutagenesis, each with complete loss of enzyme activity. The identity of a putative serine nucleophile was probed using phenyl phosphorodiamidate that was shown to be a time-dependent inhibitor of AtzF. Inhibition was due to phosphoroamidation of Ser189 as shown by liquid chromatography/matrix-assisted laser desorption ionization mass spectrometry. The modified residue corresponds in sequence alignments to the nucleophilic serine previously identified in other members of the amidase signature family. Thus, AtzF affects the cleavage of three carbon-to-nitrogen bonds via a mechanism similar to that of enzymes catalyzing single-amide-bond cleavage reactions. AtzF orthologs appear to be widespread among bacteria.  (+info)

Allophanate hydrolase, not urease, functions in bacterial cyanuric acid metabolism. (2/14)

Growth substrates containing an s-triazine ring are typically metabolized by bacteria to liberate 3 mol of ammonia via the intermediate cyanuric acid. Over a 25-year period, a number of original research papers and reviews have stated that cyanuric acid is metabolized in two steps to the 2-nitrogen intermediate urea. In the present study, allophanate, not urea, was shown to be the 2-nitrogen intermediate in cyanuric acid metabolism in all the bacteria examined. Six different experimental results supported this conclusion: (i) synthetic allophanate was shown to readily decarboxylate to form urea under acidic extraction and chromatography conditions used in previous studies; (ii) alkaline extraction methods were used to stabilize and detect allophanate in bacteria actively metabolizing cyanuric acid; (iii) the kinetic course of allophanate formation and disappearance was consistent with its being an intermediate in cyanuric acid metabolism, and no urea was observed in those experiments; (iv) protein extracts from cells grown on cyanuric acid contained allophanate hydrolase activity; (v) genes encoding the enzymes AtzE and AtzF, which produce and hydrolyze allophanate, respectively, were found in several cyanuric acid-metabolizing bacteria; and (vi) TrzF, an AtzF homolog found in Enterobacter cloacae strain 99, was cloned, expressed in Escherichia coli, and shown to have allophanate hydrolase activity. In addition, we have observed that there are a large number of genes homologous to atzF and trzF distributed in phylogenetically distinct bacteria. In total, the data indicate that s-triazine metabolism in a broad class of bacteria proceeds through allophanate via allophanate hydrolase, rather than through urea using urease.  (+info)

Purification and characterization of TrzF: biuret hydrolysis by allophanate hydrolase supports growth. (3/14)

TrzF, the allophanate hydrolase from Enterobacter cloacae strain 99, was cloned, overexpressed in the presence of a chaperone protein, and purified to homogeneity. Native TrzF had a subunit molecular weight of 65,401 and a subunit stoichiometry of alpha(2) and did not contain significant levels of metals. TrzF showed time-dependent inhibition by phenyl phosphorodiamidate and is a member of the amidase signature protein family. TrzF was highly active in the hydrolysis of allophanate but was not active with urea, despite having been previously considered a urea amidolyase. TrzF showed lower activity with malonamate, malonamide, and biuret. The allophanate hydrolase from Pseudomonas sp. strain ADP, AtzF, was also shown to hydrolyze biuret slowly. Since biuret and allophanate are consecutive metabolites in cyanuric acid metabolism, the low level of biuret hydrolase activity can have physiological significance. A recombinant Escherichia coli strain containing atzD, encoding cyanuric acid hydrolase that produces biuret, and atzF grew slowly on cyanuric acid as a source of nitrogen. The amount of growth produced was consistent with the liberation of 3 mol of ammonia from cyanuric acid. In vitro, TrzF was shown to hydrolyze biuret to liberate 3 mol of ammonia. The biuret hydrolyzing activity of TrzF might also be physiologically relevant in native strains. E. cloacae strain 99 grows on cyanuric acid with a significant accumulation of biuret.  (+info)

The structure of allophanate hydrolase from Granulibacter bethesdensis provides insights into substrate specificity in the amidase signature family. (4/14)

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Structure and function of allophanate hydrolase. (5/14)

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Control of vacuole permeability and protein degradation by the cell cycle arrest signal in Saccharomyces cerevisiae. (6/14)

Saccharomyces cerevisiae responds to deperivation of nutrients by arresting cell division at the unbudded G1 stage. Cells situated outside of G1 at the time of deperivation complete the cell cycle before arresting. This prompted an investigation of the source of nutrients used by these cells to complete division and the mechanisms controlling their availability. We found a close correlation between accumulation of unbudded cells and loss of previously formed allophanate hydrolase activity after nutrient starvation. These losses were not specific to the allantoin, system since they have been observed for a number of other enzymes and also when cellular protein levels were monitored with [3H]leucine. Loss of hydrolase activity was also observed when protein synthesis was inhibited either by addition of inhibitors or loss of the prtl gene product. We found that onset of nutrient starvation brought about release of large quantities of arginine and allantoin normally sequestered in the cell vacuole. Treatment of a cells with alpha-factor resulted in both the release of allantoin and arginine from the cell vacuole and the onset of intracellular protein degradation. These effects were not observed when either alpha cells or a/alpha diploid strains were treated with alpha-factor. These data suggest that release of vacuolar constitutents and protein turnover may be regulated by the G1 arrest signal.  (+info)

Structural analysis of the dur loci in S. cerevisiae: two domains of a single multifunctional gene. (7/14)

In Saccharomyces cerevisiae, the degradation of urea to carbon dioxide and ammonia is catalyzed by urea carboxylase and allophanate hydrolase. The loci coding for these enzymes (dur1 and dur2) are very tightly linked on the right arm of chromosome II between pet11 and met8. Pleiotropic mutations that fail to complement mutations in either of the dur loci were found to be predominantly located in or near the dur2 locus. We interpret these data as suggesting that the two dur loci might in reality be domains of a single gene that codes for a multifunctional polypeptide. In view of this conclusion, we have renamed the dur loci as the dur1,2 locus.  (+info)

Urea carboxylase and allophanate hydrolase are components of a multifunctional protein in yeast. (8/14)

Saccharomyces cerevisiae can use urea as sole nitrogen source by degrading it in two steps (urea carboxylase and allophanate hydrolase) to ammonia and carbon dioxide. We previously demonstrated that: 1) the enzymatic functions required for degradation are encoded in two tightly linked genetic loci and 2) pleiotropic mutations each resulting in the loss of both activities are found in both loci. These and other observations led to the hypothesis that urea degradation might be catalyzed by a multifunctional polypeptide. Waheed and Castric (1977) J. Biol. Chem. 252, 1628-1632), on the other hand, purified urea amidolyase from Candida utilis and reported it to be a tetramer composed of nonidentical 70- and 170-kilodalton subunits. To resolve the differing views of urea amidolyase structure, we purified the protein using rapid methods designed to avoid proteolytic cleavage. Application of these methods resulted in the isolation of a single, inducible and repressible, 204-kilodalton species. We observed no evidence for the existence of nonidentical subunits. A similar inducible, high molecular weight species was also detected in C. utilis. These biochemical results support our earlier hypothesis that urea degradation is carried out in yeast by an inducible and repressible protein composed of identical, multifunctional subunits.  (+info)

*Allophanate hydrolase

In enzymology, an allophanate hydrolase (EC 3.5.1.54) is an enzyme that catalyzes the chemical reaction allophanate + 3 H2O + ... Kanamori T, Kanou N, Kusakabe S, Atomi H, Imanaka T (2005). "Allophanate hydrolase of Oleomonas sagaranensis involved in an ATP ... Sumrada RA, Cooper TG (1982). "Urea carboxylase and allophanate hydrolase are components of a multifunctional protein in yeast ... Urea carboxylase Maitz GS, Haas EM & Castric PA (1982). "Purification and properties of the allophanate hydrolase from ...

*Urea carboxylase

Allophanate hydrolase Roon RJ; Levenberg B (1970). "ATP-Urea amidolyase (ADP) (Candida utilis)". Methods Enzymol. 17A: 317-324 ... Sumrada RA, Cooper TG (1982). "Urea carboxylase and allophanate hydrolase are components of a multifunctional protein in yeast ... allophanate). This enzyme belongs to the family of ligases, specifically those forming generic carbon-nitrogen bonds. The ...

*Urease

Urea carboxylase Allophanate hydrolase Urease test Holm L, Sander C (1997). "An evolutionary treasure: unification of a broad ...

*List of MeSH codes (D08)

... allophanate hydrolase MeSH D08.811.277.087.100 --- arylformamidase MeSH D08.811.277.087.116 --- asparaginase MeSH D08.811. ... thiolester hydrolases MeSH D08.811.277.352.897.075 --- acetyl-CoA hydrolase MeSH D08.811.277.352.897.700 --- palmitoyl-coa ... phosphoric diester hydrolases MeSH D08.811.277.352.640.050 --- annexin A3 MeSH D08.811.277.352.640.125 --- 3',5'-cyclic-GMP ... pyroglutamate hydrolase MeSH D08.811.277.087.831 --- sirtuins MeSH D08.811.277.087.902 --- urease MeSH D08.811.277.151.300 --- ...

*List of EC numbers (EC 3)

... allophanate hydrolase EC 3.5.1.55: long-chain-fatty-acyl-glutamate deacylase EC 3.5.1.56: N,N-dimethylformamidase EC 3.5.1.57: ... phloretin hydrolase EC 3.7.1.5: acylpyruvate hydrolase EC 3.7.1.6: acetylpyruvate hydrolase EC 3.7.1.7: beta-diketone hydrolase ... bile-acid-CoA hydrolase EC 3.1.2.27: choloyl-CoA hydrolase EC 3.1.2.28: 1,4-dihydroxy-2-naphthoyl-CoA hydrolase EC 3.1.2.29: ... acetyl-CoA hydrolase EC 3.1.2.2: palmitoyl-CoA hydrolase EC 3.1.2.3: succinyl-CoA hydrolase EC 3.1.2.4: 3-hydroxyisobutyryl-CoA ...
Urea amidolyase, contains both urea carboxylase and allophanate hydrolase activities, degrades urea to CO2 and NH3; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin ...
Where will your ride take you today? When you dont really know, Roam Disc is a perfect choice. Its built for any terrain, so your choices are unlimited. Smooth roads, rough roads, dirt paths or trails, Roam Disc keeps you rolling in comfort and style.. With its confident, flat handlebar positioning and lightweight ALUXX aluminum frame, Roam Disc blends all the best qualities of a mountain bike and a road bike. Its front suspension adds comfort and control. It has powerful disc brakes and durable components, so it can take some bumps on rough city roads. And the fast rolling 700c wheels can handle smooth roads or dirt, making it a great choice for town-to-trail adventures ...
am1a04d AJ229366 409 Homolog to proteins of other species than S. cerevisiae (see table 2) am1a07d AJ229367 307 am1a12d AJ229368 234 am1b02d AJ229369 236 am1b03d AJ229370 123 am1b04d AJ229371 314 7.0 E-41 YFL036w RPO41 DNA-directed RNA pol., mitochondrial am1b06d AJ229372 410 4.7 E-41 YGR208w SER2 phosphoserine phosphatase am1b11d AJ229373 327 2.1 E-42 YDL213c RNA recognition domain in the N-terminal region am1b12d AJ229374 280 am1c01d AJ229375 489 3.0 E-100 YMR229c RRP5 processing of pre-ribosomal RNA am1c05d AJ229376 275 am1c08d AJ229377 288 1.5 E-56 YDL102w CDC2 DNA-directed DNA pol. delta, catalytic 125 KD subunit am1c11d AJ229378 157 am1c12d AJ229379 145 am1d02d AJ229380 322 am1d05d AJ229381 195 repeats or mito am1d06d AJ229382 302 5.1 E-55 YBR208c DUR1,2 urea amidolyase am1d08d AJ229383 241 am1d12d AJ229384 174 am1e05r AJ229385 220 6.6 E-15 YMR287c MSU1 3-5 exonuclease for RNA 3 ss-tail, mitochondrial am1e06d AJ229386 290 am1e09r AJ229387 241 am1e10r AJ229388 138 1.7 E-06 YDL153c SAS10 ...
Roam specialty medical supply carts are mobile, maximize space and help increase efficiency. Choose from open or closed suture carts or scope carts.
b-adrenergikoi ipodoxeis, iparxoun stin kardia stous pneumones kai allou. stin kardia prokaloun au3isi tou kardiakou ri8mou kai au3isi tis sistaltikotitas tou miokardiou. (8etiki inotropos kai xronotropos drasi) stous pneumones i diegersi ton b ipodoxeon prokalei diastoli ton leion miikon inon ton brogxon =, brogxodiastoli. oi b - blockers anastelloun tous b ipodoxeis prokalontas miosi tou ri8mou kai tis dinamis sistolis ara miomeni artiriaki piesi,enw stous pneumones prokaloun sispasi ton lion miikon inon kai vrogxospasmo (pou einai enas apo tous 3 basikous paragontes tou as8matos) me lia logia, an xereis oti enas as8enis sou exei as8ma kai dias tou b-blockers, mallon en ta paeis je polla kala me tin iatriki. plus, enen kan farmako protis grammis gia tin ipertasi oi b-blockers anymore. auta, sorry giato lecture, e8imi8ika tes palies kales meres :P. ...
The zombies in World War Z are just actors - but in the animal world, zombies are a fact of life. Evolution can come up with some fiendish twists: For example, there are some species that bend other creatures to their will to keep the
We are super excited to be spending time outdoors this summer in our newly updated rooftop garden. Julie deLeon of Groundwork Design brought this beauty to life literally with her gorgeous live roof & container plantings. It started with our friend Joe Lambke, planning out the initial hardscaping, then with Jacob Shapiro & team from…
MyNorth.com is the online home of Traverse, Northern Michigans Magazine, the flagship publication of MyNorth Media, a Traverse City, Michigan company dedicated to sharing stories and photos about vacations, restaurants, wineries, the outdoors and more from Traverse City to Sleeping Bear Dunes and up to Mackinac Island.. ...
After 25 years, the U.S. Fish and Wildlife Service decided to end its program of relocating the mammals, calling the effort a failure. Fishermen complain.
Mutants (Sub 2 Mutation) - c64 remix by vurtX. Original by Fred Gray. Released 24/03/2012. Hi, this took me forever to make because I was wasnt very happy with it for a long time, but it isnt going to be getting much better so its time to let it roam free on the internet, the original SiD also reminds me of Bjorks Army of Me so I put a li...
Dev is a seasoned, creative and driven Product leader. His desire to build products for social impact brought him to Dimagi. Dev comes to Dimagi after building successful tools/products in the collaboration, content management and mobile payments space. Prior to joining Dimagi, Dev drove growth at ROAM (acquired by Ingenico), where he led their Mobile…
There are many sexually-transmitted diseases that roam the earth today. They are known to trigger various harm and damage to anybody that they strike. Because of that, everyone is warned to be careful with everything that they do.
Theres this field in which we like to play. Its isolated enough that I feel comfortable letting all three of my little folks roam. Its hard to find much open space around here, still in city limits, thats far enough away from traffic and other animals and construction and trains. ...
IT’S that time of the year again when the flu roams the streets of Glasgow and East Renfrewshire searching for fresh victims.
Weve met the 7 brothers, lets see what theyve been up to the first week. Remember, each Sim was actively played only one day, the other six days he could roam around freely. Primus ✓ finished 3 paintings ✓ painted for 5 hours - went for a jog - beat up his enemy - napped…
A good friend came to see me this evening. The nurses let me roam free after sticking this thing in my arm and we went for a CrappoBurger over the road. Company is appreciated. Spend too long alone and you just cant but think about things like spleens ...
English , Publisher: Audio CD (October 1, 2011) , ISBN-10: 1599508001 , MP3 192Kbps , 376.71 MB. The blighted aftermath of a global nuclear showdown, Deathlands exacts a blood price. The living pay it; the dead dont care. For one legendary band of warriors, this barbaric new world holds a chance for redemption: the secrets of the past. They roam a disfigured America, searching for pre-dark tech.... ...
He does not sleep much in his crib. It has been like that since he knows how to turn over. So we put him to sleep with us on the bed. Now during weekend when only me and otger kids around, he will be confined in a wooden crib. As long someone to play or talk to him, he would be fine in there. Otherwise if we let him loose, he will roam around or hurt himself. And I can go on do my chore or have a peaceful toilet moment. *wink ...
Eventbrite - Apryl Beverly, The Million-Dollar Word Stylist + W.E.R.K. Coach presents WERK Your Words: How to Write Words that Make YOU Money - Saturday, October 21, 2017 at Roam Buckhead, Atlanta, GA. Find event and ticket information.
The experts at Travel Channels Roam blog share tips on how to fly like a pro with airport hacks, in-flight beauty tricks and tips for sleeping on a plane.
Charles Back, the owner of Fairview, is also the man behind Goats do Roam, the fruit for which is sourced primarily from Paarl, Malmesbury and Paardeberg. The newly packaged Goats in Villages Viogner is now 100% Viognier with 30% barrel fermented fr...
The public should be enlightened about the opossums that roam our neighborhoods.. We found one in our backyard one night and, thinking it was a large rat, we tried to kill it. We called the animal shelter, which told us what it was. And we were pretty upset because we almost killed it.. --FERNANDO ROSALES. Downey. ...
Warning! Dinosaurs have invaded the MIT lab! They may one day "roam" through a museum near you, too.. Researchers at the Massachusetts Institute of Technology Artificial Intelligence (AI) Lab in Cambridge have created a robotic dinosaur. The lab hopes to develop small, lifelike 3D representations of dinosaurs that will be used as educational tools. The project falls under the administration of MITs Leg Lab, a division of the AI Lab dedicated to simulating and building robots that walk like their biological counterparts.. After five years of work, scientist Peter Dilworth has created a robotic model of a troodon dinosaur (see the figure). The robotic version of this creature, dubbed Troody, can walk across a desk with lifelike body movement.. Currently, Troody is one of two bipedal robots at the Leg Lab. Known as M2, the other biped simulates the legs of a human. While it can stand and walk in place, M2 cannot yet walk forward. Series elastic actuators, or springs connected to motors, are ...
How do you determine the sex of a teletubby anyway?. Teletubbies are one of the few, rare species to carry their gonads in a detachable, external sack. Therefore we know Tinky is a male because of his "purse".. This condition is only temporary, though, as Teletubbies also share their sex communaly. The "purse" belongs to each member of the clan and can be traded between members in exchange for certain favors. The troupe as a whole can be thought of as "male", though, as there are in fact no female Teletubbies, it is a distinction of trivial importance. It would be more proper to refer to Tinky as the (currently) "sexed" Teletubby, rather than male or female.. Reproduction only occurs by accident, and at this point in time, neither Teletubbies nor researchers have been able to determine its cause. Therefore, while it is safe to say that Teletubbies are homoscial they are not, by definition, homosexual.. Except for Po. Dirty, dirty Po ...
The number of patients at Brooklyns Long Island College Hospital has dwindled to 18 amid a legal stalemate between its owner, which wants to close it, and unions representing workers.
Heartbreaking footage of a starving polar bear desperately searching for food on iceless land highlights the “real face of climate change”, conservationists say.
A GIANT shark has been hooked by a builder fishing in UK waters and experts believe it is the biggest of its kind ever caught off our coast.
A couple of years ago, some 11 year old boy killed the largest wild boar anyone has ever seen. The thing is unbelievably massive. Actually, to be exact, it was "1,051 pounds and measured 9-feet-4 from the tip of its snout to the base of its tail". Seriously? No one notice this thing walking around? Its bigger than my car. And he killed it with a hand gun? Who goes hunting with a hand gun? After reading about this pig-monster, I could think about only 1 thing: how sick of eating pork this kids family is going to become. Some more pictures of such giant animals after the jump ...
Travel down any given highway in Alberta on any weekend and youre sure to see tourists pressed up against the inside of their car windows, hoping to catch a glimpse of the provinces wildlife. Often,...
Immunodeficiency. The bodys immune system is made of 3 kinds of cells: t-cells, b-cells and WBC (white blood cell). Wbcs roam the various tissues and engulf bacteria and fungi they encounter and then digest them inside themselves. When a crucial molecule, the reactive oxygen species (ros) is not present to enable the killing, the infection is not stopped. So these patients get sick very often ...
Tesla, the obnoxious green car company whose products roam the streets of Beverly Hills and carry its assorted movie stars, is about to get a lesson in sustainability if the Republican congressional tax reform bill goes through. No more free stuff. Here´s one example; Will.i.am´s custom wide-body Tesla S parked on Rodeo Drive in Beverly Hills. Highsnobiety has the details. Here´s another: Mel Gibson having a cow over a valet not bringing him back his Tesla soon enough. Daily Mail on the job. Turns out the big reason anyone buys one of these green vehicles isn´t to virtue-signal, although there´s a lot ...
Naruto woke up the next Tuesday morning to the sound of the horses wanting out. He hopped down from the loft and let them out into the field. He watched them roam around for about an hour before he heard some commotion coming from the town. He hurried to see what was going on, and in the main street was a parade of carriages and royal soldiers. Naruto couldnt see clearly so he climbed a single tree right next to the road and leaned against one of the branches. In the biggest open carriage he saw the king, who he recognized easily because every once in a while he would come to check on the horses. It was the one sitting next to the king who caught Narutos eye, a beautiful young girl with long blue hair. She sat silently at the kings side with an innocent face, looking around at the villagers.. "Wow…" Naruto gazed at her, he was so mesmerized he was not paying attention to what the king was saying.. "My people, I am pleased to announce that finally our country will forge an alliance with our ...
The undead roam Alcatraz Island as tourists struggle to survive. Even if they make it off the island, can they survive in the rest of the world ...
Though it may be enjoyable to live in an area where deer and other wildlife roam freely, theres an unfortunate downside: deer can destroy both landscapes and gardens. To create a deer-proof ...
The Misty Mountains host a lot of different kind of orc breeds. I decided to focus on the orcs (or goblins if you like) from the depths of Moria but give them and their bases a touch of the great outdoors. When night falls the orcs will roam the Misty Mountains not only the caverns and caves.. ...
This is the must read book of 2016. Family is the backbone of our society and it has lost its mojo due to traumatizing events. The Family teaches, guides, protects and nurtures and when it is broken we are all at risk. Families of the mentally ill are most vulnerable and their lived ones fill the jails, lash out, roam the streets....This book is a front line view of whiy and how this happens ...
Rad Resource: Looking for inspiration? Check out the RSA Animate videos, which are high-quality visualizations of visionary talks put on by the RSA.. Hot Tip: You dont need an art background to practice visual recording. I have some basic knowledge of composition and shapes from a couple of drawing and photography courses I took in college-quite some time ago. Really, I just started drawing in a staff meeting one day, and thus a graphic recorder was born. Graphic recorders often work off of templates to give a shape to a visualization, and the point is to populate a graphic recording with easy-to-recognize images. They do not have to be works of fine art.. Rad Resource: Wondering how to get started? You can browse Dan Roams Napkin Academy, or practice by incorporating graphic recording methods into your own note taking.. Do you have questions, concerns, kudos, or content to extend this aea365 contribution? Please add them in the comments section for this post on the aea365 webpage so that we ...
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Germany offers you vivid cultural landscapes in unspoiled nature. Take advantage of good transportation links throughout the entire country to experience the vibrant pace of metropolitan regions, or respectfully roam deserted landscapes.
My family and I absolutely love dogs! We have 3 of our own, and they are our world. We have a large house and the dogs have freedom to roam and pla...
Jim White - Burn The River Dry Lyrics. Door is locked, no ones home Frame is empty, pictures missing Throw that rock right through the window Hey, I know him, hes a singer Roam
Why are rabbits not allowed to roam freely in nature? Why do we use them to perform experiments? Rabbits are frequent victims of animal experimenters because they are mild-tempered and easy to handle, confine, and breed-more than 241,000 of them are abused in U.S. laboratories every year. ...
Im back after hobbling around the Oregon Coast the last few days so its time to write this epic. Simply saying "I completed a marathon in 4:29:00" doesnt do justice to how things really unfolded.,br /,,br /,,img id="BLOGGER_PHOTO_ID_5255257518840575250" style="DISPLAY: block; MARGIN: 0px auto 10px; CURSOR: hand; TEXT-ALIGN: center" alt="Course map" src="http://4.bp.blogspot.com/_yomUPEP6xnI/SO5s3q2xdRI/AAAAAAAAAEs/4UdLV8MGBFo/s200/08+Course.jpg" border="0" /,,br /,,img id="BLOGGER_PHOTO_ID_5255257858297788962" style="DISPLAY: block; MARGIN: 0px auto 10px; CURSOR: hand; TEXT-ALIGN: center" alt="Course elevation" src="http://1.bp.blogspot.com/_yomUPEP6xnI/SO5tLbbreiI/AAAAAAAAAE0/roam35KCJ2Q/s200/08+Course+Topo.jpg" border="0" /,,br /,The day started out well. I had a good nights sleep for the first time in days and the weather looked good. It was cloudy but dry.,br /,,br /,We got lost on the drive to the start area and arrived later than planned. The lineups for the porta potties were ...
There are many stories about the hotel and what has occurred there. All of these are written down in the guest book which of course we read through! There are stories of strange noises coming from empty rooms; coughing, labored breathing, and even voices. Sometimes these noises come from a dark corner of a guests room! Others report lights or TVs turning on and off. There are unexplained smells of flowers, dust, cigarettes and whiskey. Ghostly presences and unexplained activities lure many to this haunted hotel. Two female apparitions have been seen by numerous visitors. The first, a nurse carrying a clipboard, seems more to be a memory than an actual ghost. She roams the halls, pausing at intervals and leaning down, looking at beds that no longer exist and patients long since dead. The other, a woman in white, has been identified as a woman who died in childbirth. Neither she nor her baby left the hospital halls. According to legend she is distraught over the treatment of her dead child, who ...
... Oh, people let me tell you No matter how far I travel No matter how far I roam Theres no other girl in the whole wide world Sweet as the gi
Ive done a little digging on the plausibility of raising quail, and Im feeling hopeful. It seems they are a pleasant lot to raise but that they are very susceptible to predators (yes, even the stray cats could probably get them). This means that letting them roam freely puts the poor little guys at risk. So, if I want to raise quail, Ill need to pen them and only let them run around while were outside to protect them. We do want them to have time to walk around the gardens for their sake and because they will eat little bugs that would otherwise eat our vegetables. This setup is all very doable, but heres the catch: Muncie has a "no poultry" ordinance for residential zones of the city. People are currently working to get this overturned. Please help by signing the petition at http://www.ipetitions.com/petition/urbanbirdmuncie/. Im hoping theyll get the ordinance overturned before I build my pen and purchase my quail, but if not, Im not convinced that there isnt a loophole here. I mean, ...
This is probably the very horse that ate and buried her. What do you say? Pyrene is said to have been eaten by wild animals in the woods, where horses rarely roam ...
The Endocrine System is the series of glands that includes our thyroid, adrenals and pituitary glands; their job is to release different types of hormones into our bloodstream. When released, these hormones roam our cells and help regulate our growth,
Im still waiting to see the doctor. Im bored...so thought I would post. I didnt sleep last night and are bored out of my mind. One of the nurses from our transplant floor came up earlier this morning to visit, and I decided to walk back down with him to visit some of the other nurses. I think I spent about 1.5 hrs down there..nice break! Since Im on this particular unit, im not hooked up to the heart monitor and have free roam...which i guess is nice. I still want to either be home or on my home hospital unit. One of my nurse friends also went and got me some yummy chinese food. She should be back any minute....yay ...
A section of housing is seen in front of Machu Picchus Sacred Plaza, with the Intiwatana (Hitching Post of the Sun) above the plaza and the Western Urban Section to its right, where llamas roam ...
Nuclear Throne is a post-apocalyptic roguelike-like top-down shooter. Not the final hope of humanity post-apocalyptic, but humanity is extinct and mutants and monsters now roam the world post-apocalyptic. Fight your way through the wastelands with powerful weaponry, collecting radiation to mutate some new limbs and abilities.
In Shenanigans players are looking for the Leprechauns proverbial pot of gold at the end of the rainbow. The adventure begins, of all places, in the protagonists own modest bedroom apartment. From that point players will need to roam the streets and interact with other people during their adventur...
Our home is located on 3 acres. It is ideal for having a few chickens, a horse or two, lots of pets, a big garden, and plenty of privacy and room to roam. Located within easy commuting distance of Jackson, it is like coming home to your own private retreat every day. The modified A-frame design, back deck, and large stone fireplace bring to mind images of mountain get-aways. The house is surrounded by woods and a meadow. The great neighbors are close enough to walk to but just far enough away that you really get all the peace and quiet you crave after a busy day ...
Mens ASICS® GEL-Neo33™ :: Your quest for a new running experience is over. The menss ASICS® GEL-Neo33™ offers high performance in a light and fresh package. Encouraging a more natural flex you get the perfect combination of mild stability and cushioning in a supportive yet nimble ride. Toes get room to roam with wider, less restrictive toebox. And youll love the blister-free feel of the seamless, breathable upper. 10.2 ounces ...
On verisimilitude in role playing games….. A major issue for my campaigns is whether the ecosystems portrayed in the environments I create are plausible. Thus it is not common for my players to find vampires living next door to kobolds. Nor do gelatinous cubes roam freely around goblin lairs.. But this presents a quandary, and Shamus has neatly capsulized it in this comic. How do you provide an engaging diversity of challenges for your characters if you are trying to also create a plausible ecosystem or culture?. Are goblins likely to share their homes with mindless devouring beasts who would chow down on little goblin imps at will? Goblins are not stupid, or not that stupid anyway. Plus they are not that bad in a fight. So its unlikely that they would not either clear out their homes or else move to a new place if they couldnt get rid of the Beholder down the hallway.. The best Ive come up with is to have the kobolds and goblins use "pets" of varying sorts to protect their home, much as ...
I suppose this pre 4th of July post should have really been done in red, white and blue, but I just didnt think ahead. Oh well, I hope you have a festive holiday anyway. Im looking forward to three days off, a little trip away from home and some time for quiet contemplation. I did all the end of the week chores earlier this week so I am free to roam around this weekend. Im hoping to get some really good pictures to post on my new photography blog while Im away. For the shots I want to capture I really need a specific filter that I dont have, but Im going to see how well I can do without it. Wish me luck. Hey, nothing ventured nothing gained, right? Why dont we take a look at whats blooming in the garden ...
This is a pretty impressive bulldog! Lets hope he doesnt come alive after midnight and roam the neighborhood. The Citadel Bulldog is the logo for the Citadel Bulldog Athletic Teams. Private First Class Davis offered to pose next to him to show the scale ...
My pump is always attached right at my left hip. I hardly ever place it anywhere else except at night where it roams free! Cherry ----- Original Message ----- From: Sue Kinzelman ,email @ redacted, To: ,email @ redacted, Sent: Tuesday, August 28, 2001 8:30 AM Subject: Re: [IP] insulin , It would be interesting to find out, of the folks who have no problems , with their insulin losing its effectiveness, where do they wear the , pump? Is it right next to the body or attached somewhere cooler. , , Sue ---------------------------------------------------------- for HELP or to subscribe/unsubscribe, contact: [email protected] send a DONATION http://www.Insulin-Pumpers.org/donate.shtml ...
Researcher study silverback gorillas, one of the worlds most elusive species, in their lush Congo habitat. The goal is for scientists to get an intimate portrait of what a day in the life of these animals is like.
InTouch Health has announced that its RP-VITA (Remote Presence Virtual + Independent Telemedicine Assistant) Remote Presence Robot has been cleared by
A litter box, sometimes called a sandbox, litter tray, litter pan, or catbox, is an indoor feces and urine collection box for cats (as well as rabbits, ferrets, micro pigs; small dogs, such as Beagles and Chihuahuas; and other pets that instinctively or through training will make use of such a repository) that are permitted free roam of a home but who cannot or do not always go outside to relieve themselves. Many owners of these animals prefer not to let them roam outside for fear that they might succumb to outdoor dangers, such as weather, wildlife or traffic (it has been claimed indoor cats, on average, live ten years longer than outdoor cats). A litter box makes it possible to shelter pets from these risks. In the wild, cats naturally excrete in soft or sandy soil for easy burial. They use their paws in a backward sweeping motion to cover their feces. To stimulate this instinctive desire, a litter boxs bottom is filled typically with an inch (2.5 cm) or more of cat litter. Litter box filler ...
A squad of lipstick-sized prototypes robots developed at the University of Nebraska are being deployed to test minimally invasive surgery techniques. Specialized devices for illumination, biopsy, clamping and cauterizing are planned, some of which could be mobile in the stomach...
When Colombian soldiers fired the bullets that ended Pablo Escobars life, the country thought they were free of him. But now the infamous drug lord has come back to haunt Colombians in the...
Transport blood, plasma, biologicals, vaccines, or pharmaceuticals in a refrigerator that can run from a battery on the move. Easily carried with one hand.
Transport blood, plasma, biologicals, vaccines, or pharmaceuticals in a portable refrigerator or freezer that can run from a battery on the move.
Qoo10 - 「BIURET」 - Brand search results : At Qoo10 you will get local, and imported products at shockingly low prices! Use discount coupons and a variety of events such as time sale chance.
Patrice and I began to play. I kissed her and she tasted ever so slightly of sour milk, and there was something uniquely familiar and indeed memorable in that taste but I have still not been able to put my finger on the memory lodged deeply in the recesses of my consciousness. Patrice was utterly delectable to kiss and I had probably the most fun I have ever had kissing a woman. I could have kissed her for hours. Her urgency and enthusiasm in response to my kiss was intoxicating. I caressed her breasts, sucked on her nipples and let me hands roam over her upper body. Surprisingly she was shy about her body and at one point made me exceedingly uncomfortable by telling me that she did not like her saggy boobs and wished that she had my breasts. I did not know what to say to that half compliment and half self-criticism and continued to kiss her. It makes me sad that she felt like she needed to make excuses for her physical imperfections. We all have them and we all need to just get over ourselves! ...
This Crawlers boss roams the underground of the Hive. It respawns randomly, even up to few times a day, which makes him pretty easy to find ...
didnt see any of the feral cattle that roam the island but that may have been a good thing since some of the bulls are reported to be a bit aggressive, not a good thing if one should meet while riding a bicycle on a narrow dirt road with swamps on both sides. saw plenty of sign of them but no sightings. got thoroughly lost bicycling up a seldom used dirt road that petered out in the live oak forests several miles up the island ...
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Before Brett Favre arrived in Minnesota, Delmon Young was the most controversial player to roam the Dome. Not controversial in the same way he was in Tampa, Young hasnt generated bad ...
The Rebtel girl wants you to know theyre now offering free international mobile to mobile calls between 51 countries if both parties have the Rebtel Free Call software installed on their Android cell phones. Note this is not via WiFi but using roam anywhere 3G, but remember youll be paying data charges unless you have a flat rate plan. How do you spell disruptive technology again? Rebtel Free Call is free to download and free to use. Once installed: 1. Select an international phone number from…. Read More ...
This bison refused to abide by the signs saying Slower Traffic Use Turnouts. He was coming right at me in my lane when I swerved to miss him. Id put his weight at about 2,000 pounds, roughly the same...
Even in this community, gentrification is an ongoing issue. While there are incentives for landlords to rent to people who are homeless, in low income brackets or suffering from mental illness and who are supported by government or charitable agencies, there are often greater incentives to evict such tenants and renovate the space to rent at a higher price. Especially in neighborhoods like the Mission.. At First Glance. The first thing we notice as we walk up the carpeted stairs to the front desk is that it is incredibly bright.. The fluorescent bulbs dont flicker, but shine down with an unflattering fury onto the occupants who roam the stairs and hallways and occasionally stop by the front desk with its glass partition and signs apologizing for the inability to lend money.. The SRO. El Capitan is a medium-sized hotel. It opened around 45 years ago and has 80 rooms. Of those, about 30 percent are occupied by permanent residents, some of whom have lived here for as long as six years. None of the ...
Lyrics to Heart Breaking Baby by Jim Reeves: Heart breaking baby / What makes you want to roam / Heart breaking baby / Why dont you come
Sean Zevran is boldly bare. His lean, muscled body is on total display and his cock is ready for action. Jaxon Colt kneels naked before Sean, eager to suck. One of Jaxons hands steadies and guides Seans cock while the other freely roams Seans flawless skin and rippling muscles. Jaxons arms are tattooed, his torso is smooth, and his legs and ass are covered in fine, blond hair. That ass entices Sean to have a taste of Jaxons hole. Seans tongue delivers an intense rim job that makes Jaxon hungry for more. Jaxon puts his back into it, clearly showing his need to be fucked. Jaxons balls hang large and heavy, and Seans belly slams them with each thrust. Jaxon sucks Sean to a juicy climax while geysers of semen erupt from his cock.
He is not in the mood for shopping when we get there. He wants to roam the store, and bicycles and climbing walls are much more interesting than clothes. I talk him halfway across the store, but we stall out. He asks me to put him in a cart. That would mean going all the way back to the entrance. I decide to risk it and urge him forward to the kids clothes. We make it there, and I try to get him interested. I get one jacket on him, and its not a bad fit. He immediately starts saying "Take off!" and as I do, he hightails it for the luggage section of the store. I glance at the jackets price tag...$99. Id not have paid that anyway. Were surrounded by things that are half off, but theyre intertwined with full-price stuff. Its hard to figure out whats a good deal and whats not. I cant think about it anyway, because Ben has made a mess of the luggage and is heading towards the cookware. That could be tragic ...
I had sex on six different occasions over the last one year. I always used condoms. When I had the last encounter, the condom broke. I did not notice it until the end. I am worried and I am not sure if the same thing happened on other occasions as well. I do not know the HIV status of the partners. After this, a couple of months back, I had cold and a sore throat but this could be because I had gone to east US and was there for two weeks. It was snowing there and I had to roam around and was exposed to the cold. I am worried whether I |b|may have contracted the HIV infection|/b|. I went to private pathology laboratories and got tested for HIV using the rapid test method. I was tested negative by Tridot method twice. I also had ACON rapid test done, which too was negative. I know that these tests look for antibodies. Is there a chance of antibodies not getting produced even if there is HIV infection? I have almost completed 6 months since the last encounter. Can I assume that I have escaped from
First, lets talk about toxic overload. We ALL take in toxins in one form or another.....every single day. Over the years, these toxins get stored in our body, in fat cells, in our liver, in our lymph nodes, even in our bones. As a result, our bodys cells get damaged. These free radicals (damaged cells) roam through our body, gaining in number every day by what we breathe, put in our stomach or on our skin, and even stress. They can cause inflammation, early aging, auto-immune disorders, hormonal imbalances, and often times, these cells get so damaged that their DNA is altered and they become cancer cells. And for the most part, the body can withstand a great deal of free radicals and cancer cells. In fact, we all have cancer cells in our body right now. Its even said that we unknowingly deal with six bouts of serious cancer over the course of our lives before doctors diagnose the one we have to fight. ...
The dog must be taken to a veterinarian if you suspect it has been bitten by one of these lizards. The veterinarian will then prescribe treatment and medications. Pay close attention to the wound and report any changes. Most importantly, if you live in an area where these lizards are likely to roam, place your dog behind a fence capable of keeping the lizards out.. ...
Dying Light is a first-person, action survival game set in a vast open world. Roam a city devastated by a mysterious epidemic, scavenging for supplies and crafting weapons to help defeat the hordes of flesh-hungry enemies the plague has created.
I joined Sparkpeople in about June 2012. I had got my weight down and staying down from 76 kilo to 65 (sometimes to 60), but was not succeeding in reaching my goal, which is 60 and under. Given my eating habits when I travel and cant control what food is put before me, and dont control the portions I eat, I need to have a normal weight of 57, to keep at 60 and under. (DONE March 2013!) I am learning to enjoy Sparkpeoples encouragement about exercise, and much appreciate their information about nutrition, fitness etc. I live in Albania, which is challenging, both for finding healthy food, particularly low fat, and for exercising (savage dogs roam loose or get out. I got badly bitten by the neighbours Alsatian and the doctor didnt have enough sutures to give me more than 3 stitches). Also snow and ice, or thick mud afterwards are not conducive to walking - I strained my knee last winter... This winter has been mild and I can easily get out with the dogs most days ...
Kevin Park is a "man" who savagely murdered his girlfriends 2 year old Daughter in April 2014. The officer in charge commented that it was the worst thing he had ever seen. Once again, the details are too horrific to go into but the defenceless little girl, who was not even a metre tall, had over 65 separate injuries to her tiny body. A fractured skull, tears to her liver, the list goes on. The piece of scum was convicted. He got a life sentence. Which as anyone who lives in the UK knows, most of the time isnt actually a life sentence. He will be up for parole in 22 years. When hes 49. The family said they are"pleased justice has been served." Justice hasnt been served. In 22 years, he could be free to roam the streets again. Free to pop down the shop for a pint of milk. Free to possibly have more children. That innocent little girl who trusted him to look after her will still be dead. She never made it to her first day of school. Never got to make her friends for life. Watch her favourite ...
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When I was a fourth grader, we had a cat. Dad made Tiger a little hanging door in a basement window, like a dog door, and she could come and go as she pleased. Tiger would go out in the morning, and she would play in our backyard.. One day, my parents told me that our neighbors were mad at us because Tiger was killing their chickens. This was obviously not true - Tiger wouldnt do that, and besides, the chickens were bigger than her. And my parents didnt like those neighbors anyway. Innocent Tiger continued to roam.. One night, I heard Tiger screaming in the basement. I was too young for bravery, and got into bed with my mom, who assured me that Dad would take care of things when he got home. Mom seemed too scared to go downstairs too. I must have finally fallen asleep, and didnt see Dad until the morning. When I came downstairs, he was washing the shovel in the laundry tub. He had buried Tiger.. Tiger had been bleeding from the mouth, there was blood all over the basement floor, and I ...
Due gemelle a Roma (When in Rome) è un film del 2002 diretto da Steve Purcell con Mary-Kate e Ashley Olsen. Due sorelle, Charlie e Leyla, si recano a Roma per lavorare. Quando tutto sembra andare per il verso giusto, vengono licenziate. Leyla ha la passione per la fotografia mentre Charlie è interessata alla moda. Dopo essere state licenziate incontrano il presidente dellazienda di moda, Derek Hammond, che permette loro di continuare a lavorare. Quando però gli abiti della casa di moda a loro affidati spariscono, le due sorelle scoprono che il colpevole è Enrico Tortoni, loro responsabile, e con laiuto di Derek riescono a incastrarlo. Roam (Scritta da Catherine E. Pierson e Fred Schneider) Cantata da The B-52s Everywhere She Goes (Scritta da Jess Cayes e Ricky Jackson) Cantata da Motor There You Are (Scritta da Carolyn Arends) Cantata da Carolyn Arends These Are Days (Scritta da Natalie Merchant e Rob Buck) Cantata da 10.000 Maniacs Life Is Good (Scritta da Lloyd Neal) Cantata da Ritalin ...
Lets talk house, more specifically deep house. Its a kind of music that prides itself on evolving melodies and a subtleness that leave you feeling downright sexy as you sway back and forth to its intrinsic sensuality. We got some boys over in Hamburg that are doing it right and theyre called Smallpeople. Consisting of Julius Steinhoff and Just von Ahlefeld (AKA Dionne), the duo are owners of the Smallville Records label and record store. Smallville has been making a name for itself as one of the most premiere deep house imprints with diverse releases from artists like STL, Thomas Melchior, and Move D. I digress, lets pivot back to the subjects at hand.. Smallpeople are disappointing nobody with their sumptuous debut full-length, Salty Days. The LP is extremely deep with an epicurean kind of sound that for some might be a little too chill but for the attentive listener is a treat of quirky samples and dreamy synths. You will find yourself blissed out as you aurally roam through this expertly ...
Lets talk house, more specifically deep house. Its a kind of music that prides itself on evolving melodies and a subtleness that leave you feeling downright sexy as you sway back and forth to its intrinsic sensuality. We got some boys over in Hamburg that are doing it right and theyre called Smallpeople. Consisting of Julius Steinhoff and Just von Ahlefeld (AKA Dionne), the duo are owners of the Smallville Records label and record store. Smallville has been making a name for itself as one of the most premiere deep house imprints with diverse releases from artists like STL, Thomas Melchior, and Move D. I digress, lets pivot back to the subjects at hand.. Smallpeople are disappointing nobody with their sumptuous debut full-length, Salty Days. The LP is extremely deep with an epicurean kind of sound that for some might be a little too chill but for the attentive listener is a treat of quirky samples and dreamy synths. You will find yourself blissed out as you aurally roam through this expertly ...
GUNNED down by robbers in Gauteng, Senzo Meyiwa had risen from a scruffy Umlazi township street, where chickens still roam, to become Bafana Bafana captain ...
She loves to roam around in it and sniff after the various bugs. The grasshoppers always amuse her this time of the year. I used to freak out when shes run around in the garden, but I dont mind anymore. My plants are very forgiving and can take her ...
We have seen it so many times in the movies - Computers getting smarter than humans and ultimately trying to exterminate us so they can roam free in the wo
Penguin here.. You may have read our earlier post on hyraxes. The rock hyrax roams through Africa and the Middle East, living in rocky areas to more easily escape predators. They normally remain in groups of 10-80 individuals, where there is typically one dominant male, 3 to 15 related adult females and many offspring. You can read about all this and more on Wikipedias Rock Hyrax article.. Found here: m4m2012. ...
Now that dinosaurs no longer roam the Earth, scientists use modern animals to understand how their ancient ancestors reproduced, walked, and even how they regulated their body temperature. While researchers continue to debate ...
I have been a homeless advocate in the city of Taunton for nearly five years. I encounter several homeless individuals on a daily basis. I park my car in an area where the homeless often roam. I distribute warm hats, gloves, socks, coats, blankets as well as water and snacks from my car. I have come to know nearly every homeless person in the city. I am able to provide this outreach thanks to in-kind donations from friends near and far.
The other problem is that so much water makes him tinkle so much. I can´t stand the smell so I will scoop the saturated bedding out of his cage each day when I get home and replace it with fresh bedding. But I can´t let him roam free because he leaks all over the carpet. He gets so jealous of Spot who will wander around the room while I´m cleaning or feeding. And I have to limit his lap time to 10-15 minutes or I get peed on ...
A medical solution is developed so that zombies no longer need to roam the world and eat human brains - they are restored to functioning human beings (in the main). The way society deals with this is through the magic of political correctness: there are no zombies any more; instead they are the victims of an awful affliction known as PDS - Partially Deceased Syndrome. As long as these "PDS sufferers" take their daily medication, and use contact lenses and cover-up to look less conspicuous, they can re-integrate into society ...
By NABANITA DUTT Their face is their fortune. Caked in cheap rouge, kajal, powder and lipstick, they dress in ill-fitting blouses and colorful saris in a grotesque parody of womanhood as they roam the busy marketplaces in groups, terrorizing pedestrians, hustling for ten or a hundred rupees. These are not
Now that Shaun of the Dead turned the zombie apocalypse into a British comedy, the idea of a funny zombie movie might not sound so original, but the makers of Zombieland want to assure you that they have a unique approach. In the upcoming horror comedy, survivors roam the desolate American landscape dispatching the brain-eating undead-and clean their hands with Purell afterwards. Director Ruben Fleischer called Zombieland more of an American zombie comedy. "Shaun of the Dead is really funny, and I guess thats its closest kindred spirit as far as its approach to the zombie apocalypse, but I think that this is much more grounded, an American version of that," Fleischer said in a press conference last month at Comic-Con in San Diego. "I would say its more of a comedy than it is a horror-zombie movie. Its really about these characters and their unique experiences, and the zombies are somewhat of a backdrop for it all.". In the movie, directed by newbie Fleischer, Woody Harrelson plays seasoned ...
Looking for online definition of biuret reaction in the Medical Dictionary? biuret reaction explanation free. What is biuret reaction? Meaning of biuret reaction medical term. What does biuret reaction mean?
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Looking for online definition of cyanuric acid in the Medical Dictionary? cyanuric acid explanation free. What is cyanuric acid? Meaning of cyanuric acid medical term. What does cyanuric acid mean?
0043] There can be used as the polyisocyanate component A) the conventional aliphatic, cycloaliphatic and in particular aromatic di- and/or poly-isocyanates. Examples of such suitable polyisocyanates are 1,4-butylene diisocyanate, 1,5-pentane diisocyanate, 1,6-hexamethylene diisocyanate (HDI), isophorone diisocyanate (IPDI), 2,2,4- and/or 2,4,4-trimethylhexamethylene diisocyanate, the isomeric bis(4,4-isocyanatocyclohexyl)methanes or mixtures thereof of any desired isomer content, 1,4-cyclohexylene diisocyanate, 1,4-phenylene diisocyanate, 2,4- and/or 2,6-toluene diisocyanate (TDI), 1,5-naphthylene diisocyanate, 2,2- and/or 2,4- and/or 4,4-diphenylmethane diisocyanate (MDI) and/or higher homologues (pMDI), 1,3- and/or 1,4-bis-(2-isocyanato-prop-2-yl)-benzene (TMXDI), 1,3-bis-(isocyanatomethyl)benzene (XDI). In addition to the polyisocyanates mentioned above, modified polyisocyanates with a uretdione, isocyanurate, urethane, carbodiimide, uretonimine, allophanate or biuret structure can also ...
0059] Polyisocyanates (or isocyanate precursors) suitable for use in the process of this invention include aliphatic, alicyclic, arylaliphatic, aromatic, or heterocyclic polyisocyanates, or combinations thereof. Any polyisocyanate that is suitable for use in the production of polymeric foams can be utilized. Of particular importance are aromatic diisocyanates such as toluene and diphenylmethane diisocyanates in pure, modified, or crude form. MDI variants (diphenylmethane diisocyanate modified by the introduction of urethane, allophanate, urea, biuret, carbodiimide, uretonimine, or isocyanurate residues) and the mixtures of diphenylmethane diisocyanates and oligomers thereof known in the art as crude or polymeric MDI (polymethylene polyphenylene polyisocyanates) are especially useful. Representative examples of suitable polyisocyanates include ethylene diisocyanate, 1,4-tetramethylene diisocyanate, 1,6-hexamethylene diisocyanate, trimethyl hexamethylene diisocyanate, 1,1,2-dodecane diisocyanate, ...
Someone once told me that roads in a forest are like veins - once opened they can end up draining the forest of life. When APP builds a concession in tiger habitat, it not only destroys the forest the tiger would normally roam, sleep and hunt in - the roads it builds to carry away the timber also let in all kinds of other disruptions. People come and lay traps to hunt other animals, and even if they dont mean to interfere with the tiger, they do. When their traditional habitat is destroyed tigers must roam even further for food and shelter, which often brings them closer to forest communities and causes more conflict.. APP must stop relying on further clearance of rainforest for its business. Destruction of rainforests in Indonesia must stop to protect animals like the Sumatran tiger, minimize the negative consequences for forest communities, and for Indonesia to be able to cut its carbon emissions contributing to climate change, most of which come from deforestation ...
Many cancers are deadly because the malignant cells metastasize to distant regions of the body. Metastases to particular organs can involve the cancer cell expressing receptors for a chemokine produced by the target tissue, but the mechanisms whereby cancer cells are triggered to express such "molecular homing devices" are unclear. Hypoxia--which occurs when tumors outgrow their blood supply--provides a metastatic trigger through the transcription factor HIF (hypoxia-inducible factor). When oxygen supply is normal, the protein product of the von Hippel-Lindau tumor suppressor gene (pVHL) participates in degradation of the HIF α subunit; during hypoxia, HIF levels rise, as do the levels of HIF target genes. Staller et al. used DNA microarray analysis to identify genes regulated by pVHL, which is inactivated in various cancers. When transfected into pVHL-deficient renal cell carcinoma (RCC) cells, pVHL suppressed expression of CXCR4 (a chemokine receptor associated with bone metastases in breast ...

Allophanate hydrolase - WikipediaAllophanate hydrolase - Wikipedia

In enzymology, an allophanate hydrolase (EC 3.5.1.54) is an enzyme that catalyzes the chemical reaction allophanate + 3 H2O + ... Kanamori T, Kanou N, Kusakabe S, Atomi H, Imanaka T (2005). "Allophanate hydrolase of Oleomonas sagaranensis involved in an ATP ... Sumrada RA, Cooper TG (1982). "Urea carboxylase and allophanate hydrolase are components of a multifunctional protein in yeast ... Urea carboxylase Maitz GS, Haas EM & Castric PA (1982). "Purification and properties of the allophanate hydrolase from ...
more infohttps://en.wikipedia.org/wiki/Allophanate_hydrolase

KEGG BRITE: Enzymes - Equus caballus (horse)KEGG BRITE: Enzymes - Equus caballus (horse)

3.5.1.54 allophanate hydrolase 3.5.1.55 long-chain-fatty-acyl-glutamate deacylase ... 3.5.1.101 L-proline amide hydrolase 3.5.1.102 2-amino-5-formylamino-6-ribosylaminopyrimidin-4(3H)-one 5-monophosphate ...
more infohttp://www.genome.jp/kegg-bin/get_htext?ecb01000+100070281

Urea carboxylase - WikipediaUrea carboxylase - Wikipedia

Allophanate hydrolase Roon RJ; Levenberg B (1970). "ATP-Urea amidolyase (ADP) (Candida utilis)". Methods Enzymol. 17A: 317-324 ... Sumrada RA, Cooper TG (1982). "Urea carboxylase and allophanate hydrolase are components of a multifunctional protein in yeast ... allophanate). This enzyme belongs to the family of ligases, specifically those forming generic carbon-nitrogen bonds. The ...
more infohttps://en.wikipedia.org/wiki/Urea_carboxylase

Frontiers | Reconstruction of the Metabolic Potential of Acidophilic Sideroxydans Strains from the Metagenome of an...Frontiers | Reconstruction of the Metabolic Potential of Acidophilic Sideroxydans Strains from the Metagenome of an...

This pathway involves urea carboxylase and allophanate hydrolase activity thought to be also involved in urea degradation ( ... Kanamori, T., Kanou, N., Kusakabe, S., Atomi, H., and Imanaka, T. (2005). Allophanate hydrolase of Oleomonas sagaranensis ...
more infohttps://www.frontiersin.org/articles/10.3389/fmicb.2016.02082/full

KEGG SSDB Best Search Result: bdi:100835291KEGG SSDB Best Search Result: bdi:100835291

cyn:Cyan7425_3252 allophanate hydrolase K01457 607 100 ( -) 29 0.300 150 -, 1 fjg:BB050_03756 2-oxoisovalerate dehydrogenase ... ase:ACPL_5595 alpha/beta hydrolase fold protein 255 109 ( 7) 31 0.330 103 -, 2 asf:SFBM_0853 3-oxoacyl-[acyl-carrier-protein] ... taa:NMY3_01686 Alpha/beta hydrolase family protein K06889 346 104 ( -) 30 0.324 71 -, 1 tne:Tneu_1901 Radical SAM domain ...
more infohttp://www.kegg.jp/ssdb-bin/ssdb_best?org_gene=bdi:100835291

KEGG SSDB Best Search Result: mhi:Mhar 2265KEGG SSDB Best Search Result: mhi:Mhar 2265

ror:RORB6_06485 allophanate hydrolase K01457 599 102 ( -) 29 0.301 73 -, 1 rro:104674625 early B-cell factor 4 K09103 645 102 ... sphk:SKP52_11960 glycosyl hydrolase family protein K05349 877 105 ( -) 30 0.307 189 ,-, 1 syc:syc0479_d oxidoreductase aldo/ ... scb:SCAB_25521 putative glycosyl hydrolase K01811 683 100 ( -) 29 0.316 79 -, 1 smt:Smal_3299 phosphoesterase PA-phosphatase ... rmu:RMDY18_03680 Zn-dependent hydrolase including glyox 322 112 ( -) 31 0.320 103 -, 1 rpe:RPE_0024 gamma-glutamyltransferase ...
more infohttp://www.kegg.jp/ssdb-bin/ssdb_best?org_gene=mhi:Mhar_2265

Toxicity, degradation and analysis of the herbicide atrazine | Springer for Research & DevelopmentToxicity, degradation and analysis of the herbicide atrazine | Springer for Research & Development

Cheng G, Shapir N, Sadowsky MJ, Wackett LP (2005) Allophanate hydrolase, not urease, functions in bacterial cyanuric acid ...
more infohttps://rd.springer.com/article/10.1007/s10311-017-0665-8

SMART: Secondary literature for Biotin carb C domainSMART: Secondary literature for Biotin carb C domain

Urea amidolyase catalyzes the two reactions (urea carboxylase and a allophanate hydrolase) associated with urea degradation in ... These data are consistent with the suggestion that sequential induction of allophanate hydrolase and urea carboxylase ... we determined whether or not a detectable period of time elapsed between the appearance of allophanate hydrolase activity and ... approximately 2 to 6 min elapsed between the appearance of allophanate hydrolase and urea carboxylase activities. In search of ...
more infohttp://smart.embl-heidelberg.de/smart/show_secondary.cgi?domain=Biotin_carb_C

Multi-targeted priming for genome-wide gene expression assays | BMC Genomics | Full TextMulti-targeted priming for genome-wide gene expression assays | BMC Genomics | Full Text

... contains both urea carboxylase and allophanate hydrolase activities. It also converts allantoin to ammonia and carbon dioxide, ...
more infohttps://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-11-477

Psed 3186 protein (Pseudonocardia dioxanivorans) - STRING interaction networkPsed 3186 protein (Pseudonocardia dioxanivorans) - STRING interaction network

Isochorismatase hydrolase. Allophanate hydrolase. 0.914. Psed_3186. Psed_3185. Psed_3186. Psed_3185. Isochorismatase hydrolase ...
more infohttps://string-db.org/network/675635.Psed_3186

Noca 0093 protein (Nocardioides sp. JS614) - STRING interaction networkNoca 0093 protein (Nocardioides sp. JS614) - STRING interaction network

Allophanate hydrolase subunit 1. 0.565. Noca_0048. Noca_2978. GntR family transcriptional regulator ...
more infohttps://string-db.org/network/196162.Noca_0093

Network Portal - Gene RSP 3450Network Portal - Gene RSP 3450

Allophanate hydrolase subunit 1. cog/ cog. peptidyl-prolyl cis-trans isomerase activity. go/ molecular_function. protein ... Predicted hydrolase of the metallo-beta-lactamase superfamily (NCBI). 84, 361. RSP_2599. phoB. Phosphate regulon ...
more infohttp://networks.systemsbiology.net/rsp/gene/RSP_3450

Structure and function of biotin-dependent carboxylases | SpringerLinkStructure and function of biotin-dependent carboxylases | SpringerLink

... a KipI-KipA homolgue incorrectly annotated as an allophanate hydrolase. Acta Cryst D67:105-111Google Scholar ...
more infohttps://link.springer.com/article/10.1007%2Fs00018-012-1096-0

EC 3.5.1EC 3.5.1

... 54 allophanate hydrolase. EC 3.5.1.55 long-chain-fatty-acyl-glutamate deacylase. EC 3.5.1.56 N,N-dimethylformamidase. ... EC 3.5.1.99 fatty acid amide hydrolase EC 3.5.1.100 (R)-amidase EC 3.5.1.101 L-proline amide hydrolase EC 3.5.1.102 2-amino-5- ... EC 3.5.1.64 Nα-benzyloxycarbonylleucine hydrolase. EC 3.5.1.65 theanine hydrolase. EC 3.5.1.66 2-(hydroxymethyl)-3-( ... EC 3.5.1.90 adenosylcobinamide hydrolase. EC 3.5.1.91 N-substituted formamide deformylase. EC 3.5.1.92 pantetheine hydrolase. ...
more infohttp://www.sbcs.qmul.ac.uk/iubmb/enzyme/EC3/5/1/

SAOUHSC 01710 - AureoWikiSAOUHSC 01710 - AureoWiki

Allophanate hydrolase 2 and Biotin carboxylase cluster Biotin carboxyl carrier protein *PFAM: Hybrid (CL0105) Biotin_lipoyl; ...
more infohttp://aureowiki.med.uni-greifswald.de/SAOUHSC_01710

Complete genome sequence and metabolic potential of the quinaldine-degrading bacterium Arthrobacter sp. Rue61a | BMC Genomics |...Complete genome sequence and metabolic potential of the quinaldine-degrading bacterium Arthrobacter sp. Rue61a | BMC Genomics |...

... allophanate) catalyzed by urea carboxylase, followed by hydrolysis to CO2 and ammonia by allophanate hydrolase (see Additional ... Two pathways seem to be present for the degradation of urea, namely, direct cleavage by urease (ureABC), and an allophanate ... The genome contains several genes coding for putative lipases/esterases of the α/β-hydrolase fold-superfamily or the SGNH- ... Urate degradation probably involves urate oxidase and 5-hydroxyisourate hydrolase. The next step, catalyzed by OHCU ...
more infohttps://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-13-534

SAOUHSC 01709 - AureoWikiSAOUHSC 01709 - AureoWiki

Allophanate hydrolase 2 and Biotin carboxylase cluster Biotin carboxylase (EC 6.3.4.14) ...
more infohttp://aureowiki.med.uni-greifswald.de/SAOUHSC_01709

Compositions and Methods for Modeling Saccharomyces cerevisiae Metabolism - Patent applicationCompositions and Methods for Modeling Saccharomyces cerevisiae Metabolism - Patent application

... allophanate hydrolase YBR208C 3.5.1.54 DUR1 Allophanate hydrolase UREAC -, 2 NH3 + 2 CO2 dur2 YJL126W 3.5.5.1 NIT2 nitrilase ... YBL015W 3.1.2.1 ACH1 acetyl CoA hydrolase COA + AC -, ACCOA ach1_1 YBL015W 3.1.2.1 ACH1 acetyl CoA hydrolase COAm + ACm -, ... S-Formylglutathione hydrolase FGT ,-, RGT + FOR unkrx11 YGR087C 4.1.1.1 PDC6 pyruvate decarboxylase PYR -, CO2 + ACAL pdc6 ... hydrolase) YBL098W 1.14.13.9 YBL098W Kynurenine 3-hydroxylase, NADPH-dependent KYN + NADPH + O2 -, HKYN + NADP kmo flavin ...
more infohttp://www.patentsencyclopedia.com/app/20100280803

Docphin | Medical Research Articles Published in October 2014 | Page 120 of 120Docphin | Medical Research Articles Published in October 2014 | Page 120 of 120

X-ray structure of the amidase domain of AtzF, the allophanate hydrolase from the cyanuric acid-mineralizing multienzyme ...
more infohttps://www.docphin.com/medical-research-articles-published-in-month?month=10&year=2014&page=120

51784 bearing,51784 sale in Botswana51784 bearing,51784 sale in Botswana

25 Jan 2001 bearing atzE and atzF were shown to encode a biuret hydrolase and allophanate hydrolase, 51784 54699 d Transposase ...
more infohttp://fluoridefacts.org/8209/51784-bearing%2C51784-sale-in-Botswana/

Genetics Institute   » UFGI publication round-up week 9/25, 10/2, 10/9 and 10/16/17Genetics Institute » UFGI publication round-up week 9/25, 10/2, 10/9 and 10/16/17

... and homologs of allophanate hydrolase subunits (renamed pxpB and pxpC). Inactivation of Bacillus subtilis pxpA, pxpB, or pxpC ...
more infohttp://ufgi.ufl.edu/ufgi-publication-round-up-week-925-102-109-and-101617/

Atrazine induces complete feminization and chemical castration in male African clawed frogs (Xenopus laevis) | PNASAtrazine induces complete feminization and chemical castration in male African clawed frogs (Xenopus laevis) | PNAS

X-Ray Structure of the Amidase Domain of AtzF, the Allophanate Hydrolase from the Cyanuric Acid-Mineralizing Multienzyme ...
more infohttp://www.pnas.org/content/107/10/4612

Draft genome sequence of the type strain of the sulfur-oxidizing acidophile, Acidithiobacillus albertensis (DSM 14366) |...Draft genome sequence of the type strain of the sulfur-oxidizing acidophile, Acidithiobacillus albertensis (DSM 14366) |...

... allophanate hydrolase and the urea ABC transporter encoding genes, d) the spermidine/putrescine ABC transporter potABC and e) ... tetrathionate hydrolase (tetH, doxD) and heterodisulfide reductase (hdrBC and hdrABC) previously found in A. thiooxidans T and ...
more infohttps://standardsingenomics.biomedcentral.com/articles/10.1186/s40793-017-0282-y

ENZYME: 3.-.-.ENZYME: 3.-.-.

Allophanate hydrolase 3.5.1.55 Long-chain-fatty-acyl-glutamate deacylase 3.5.1.56 N,N-dimethylformamidase 3.5.1.57 ... Acetyl-CoA hydrolase 3.1.2.2 Palmitoyl-CoA hydrolase 3.1.2.3 Succinyl-CoA hydrolase 3.1.2.4 3-hydroxyisobutyryl-CoA hydrolase ... hydrolase 3.3.2.7 Hepoxilin-epoxide hydrolase 3.3.2.8 Limonene-1,2-epoxide hydrolase 3.3.2.9 Microsomal epoxide hydrolase 3.3. ... Phloretin hydrolase 3.7.1.5 Acylpyruvate hydrolase 3.7.1.6 Acetylpyruvate hydrolase 3.7.1.7 Beta-diketone hydrolase 3.7.1.8 2,6 ...
more infohttps://enzyme.expasy.org/EC/3.-.-.-
  • In enzymology, an allophanate hydrolase (EC 3.5.1.54) is an enzyme that catalyzes the chemical reaction allophanate + 3 H2O + H+ ⇌ {\displaystyle \rightleftharpoons } 2 HCO3− + 2 NH4+ Thus, the two substrates of this enzyme are allophanate (urea-1-carboxylate or N-carbamoylcarbamate) and H2O, whereas its two products are HCO3− and NH4+. (wikipedia.org)
  • In enzymology, an urea carboxylase (EC 6.3.4.6) is an enzyme that catalyzes the chemical reaction ATP + urea + HCO3- ⇌ {\displaystyle \rightleftharpoons } ADP + phosphate + urea-1-carboxylate The 3 substrates of this enzyme are ATP, urea, and HCO3-, whereas its 3 products are ADP, phosphate, and urea-1-carboxylate (allophanate). (wikipedia.org)