Leviviridae: A family of bacteriophages that infects enterobacteria, CAULOBACTER, and PSEUDOMONAS. The genome consists of linear, positive-sense single-stranded RNA.Allolevivirus: A bacteriophage genus of the family LEVIVIRIDAE, whose viruses contain the longer version of the genome and have no separate cell lysis gene.Encyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)RNA Viruses: Viruses whose genetic material is RNA.Q beta Replicase: An enzyme that catalyzes the replication of the RNA of coliphage Q beta. EC 2.7.7.-.Enterobacteriaceae: A family of gram-negative, facultatively anaerobic, rod-shaped bacteria that do not form endospores. Its organisms are distributed worldwide with some being saprophytes and others being plant and animal parasites. Many species are of considerable economic importance due to their pathogenic effects on agriculture and livestock.RNA, Viral: Ribonucleic acid that makes up the genetic material of viruses.Virus Replication: The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.RNA Phages: Bacteriophages whose genetic material is RNA, which is single-stranded in all except the Pseudomonas phage phi 6 (BACTERIOPHAGE PHI 6). All RNA phages infect their host bacteria via the host's surface pili. Some frequently encountered RNA phages are: BF23, F2, R17, fr, PhiCb5, PhiCb12r, PhiCb8r, PhiCb23r, 7s, PP7, Q beta phage, MS2 phage, and BACTERIOPHAGE PHI 6.Terminology as Topic: The terms, expressions, designations, or symbols used in a particular science, discipline, or specialized subject area.Indigo Carmine: Indolesulfonic acid used as a dye in renal function testing for the detection of nitrates and chlorates, and in the testing of milk.BooksClassification: The systematic arrangement of entities in any field into categories classes based on common characteristics such as properties, morphology, subject matter, etc.Red Cross: International collective of humanitarian organizations led by volunteers and guided by its Congressional Charter and the Fundamental Principles of the International Red Cross Movement, to provide relief to victims of disaster and help people prevent, prepare for, and respond to emergencies.Vanilla: A plant genus of the family ORCHIDACEAE that is the source of the familiar flavoring used in foods and medicines (FLAVORING AGENTS).Paeonia: A plant genus of the family Paeoniaceae, order Dilleniales, subclass Dilleniidae, class Magnoliopsida. These perennial herbs are up to 2 m (6') tall. Leaves are alternate and are divided into three lobes, each lobe being further divided into three smaller lobes. The large flowers are symmetrical, bisexual, have 5 sepals, 5 petals (sometimes 10), and many stamens.Bacteria: One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.Editorial Policies: The guidelines and policy statements set forth by the editor(s) or editorial board of a publication.Authorship: The profession of writing. Also the identity of the writer as the creator of a literary production.Patents as Topic: Exclusive legal rights or privileges applied to inventions, plants, etc.Bacteriophages: Viruses whose hosts are bacterial cells.Inventions: A novel composition, device, or process, independently conceived de novo or derived from a pre-existing model.Biological Therapy: Treatment of diseases with biological materials or biological response modifiers, such as the use of GENES; CELLS; TISSUES; organs; SERUM; VACCINES; and humoral agents.Culture Media: Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.Bacteriophage T4: Virulent bacteriophage and type species of the genus T4-like phages, in the family MYOVIRIDAE. It infects E. coli and is the best known of the T-even phages. Its virion contains linear double-stranded DNA, terminally redundant and circularly permuted.Pseudomonas Phages: Viruses whose host is Pseudomonas. A frequently encountered Pseudomonas phage is BACTERIOPHAGE PHI 6.Bacteriophage lambda: A temperate inducible phage and type species of the genus lambda-like viruses, in the family SIPHOVIRIDAE. Its natural host is E. coli K12. Its VIRION contains linear double-stranded DNA with single-stranded 12-base 5' sticky ends. The DNA circularizes on infection.Coliphages: Viruses whose host is Escherichia coli.Biotechnology: Body of knowledge related to the use of organisms, cells or cell-derived constituents for the purpose of developing products which are technically, scientifically and clinically useful. Alteration of biologic function at the molecular level (i.e., GENETIC ENGINEERING) is a central focus; laboratory methods used include TRANSFECTION and CLONING technologies, sequence and structure analysis algorithms, computer databases, and gene and protein structure function analysis and prediction.

Spontaneous rearrangements in RNA sequences. (1/58)

The ability of RNAs to spontaneously rearrange their sequences under physiological conditions is demonstrated using the molecular colony technique, which allows single RNA molecules to be detected provided that they are amplifiable by the replicase of bacteriophage Qbeta. The rearrangements are Mg2+-dependent, sequence-non-specific, and occur both in trans and in cis at a rate of 10(-9) h(-1) per site. The results suggest that the mechanism of spontaneous RNA rearrangements differs from the transesterification reactions earlier observed in the presence of Qbeta replicase, and have a number of biologically important implications.  (+info)

CCA initiation boxes without unique promoter elements support in vitro transcription by three viral RNA-dependent RNA polymerases. (2/58)

It has previously been observed that the only specific requirement for transcriptional initiation on viral RNA in vitro by the RNA-dependent RNA polymerase (RdRp) of turnip yellow mosaic virus is the CCA at the 3' end of the genome. We now compare the abilities of this RdRp, turnip crinkle virus RdRp, and Qbeta replicase, an enzyme capable of supporting the complete viral replication cycle in vitro, to transcribe RNA templates containing multiple CCA boxes but lacking specific viral sequences. Each enzyme is able to initiate transcription from several CCA boxes within these RNAs, and no special reaction conditions are required for these activities. The transcriptional yields produced from templates comprised of multiple CCA or CCCA repeats relative to templates derived from native viral RNA sequences vary between 2:1 and 0.1:1 for the different RdRps. Control of initiation by such redundant sequences presents a challenge to the specificity of viral transcription and replication. We identify 3'-preferential initiation and sensitivity to structural presentation as two specificity mechanisms that can limit initiation among potential CCA initiation sites. These two specificity mechanisms are used to different degrees by the three RdRps. The finding that three viral RdRps representing two of the three supergroups within the positive-strand RNA viral RdRp phylogeny support substantial transcription in the absence of unique promoters suggests that this phenomenon may be common among positive-strand viruses. A framework is presented arguing that replication of viral RNA in the absence of unique promoter elements is feasible.  (+info)

Mutilation of RNA phage Qbeta virus-like particles: from icosahedrons to rods. (3/58)

Icosahedral virus-like particles (VLPs) of RNA phage Qbeta are stabilized by four disulfide bonds of cysteine residues 74 and 80 within the loop between beta-strands F and G (FG loop) of the monomeric subunits, which determine the five-fold and quasi-six-fold symmetry contacts of the VLPs. In order to reduce the stability of Qbeta VLPs, we mutationally converted the amino acid stretch 76-ANGSCD-81 within the FG loop into the 76-VGGVEL-81 sequence. It led to production in Escherichia coli cells of aberrant rod-like Qbeta VLPs, along with normal icosahedral capsids. The length of the rod-like particles exceeded 4-30 times the diameter of icosahedral Qbeta VLPs.  (+info)

A protein antibiotic in the phage Qbeta virion: diversity in lysis targets. (4/58)

A(2), a capsid protein of RNA phage Qbeta, is also responsible for host lysis. A(2) blocked synthesis of murein precursors in vivo by inhibiting MurA, the catalyst of the committed step of murein biosynthesis. An A(2)-resistance mutation mapped to an exposed surface near the substrate-binding cleft of MurA. Moreover, purified Qbeta virions inhibited wild-type MurA, but not the mutant MurA, in vitro. Thus, the two small phages characterized for their lysis strategy, Qbeta and the small DNA phage phiX174, effect host lysis by targeting different enzymes in the multistep, universally conserved pathway of cell wall biosynthesis.  (+info)

Functional replacement of the Escherichia coli hfq gene by the homologue of Pseudomonas aeruginosa. (5/58)

The 102 aa Hfq protein of Escherichia coli (Hfq(Ec)) was first described as a host factor required for phage Qbeta replication. More recently, Hfq was shown to affect the stability of several E. coli mRNAs, including ompA mRNA, where it interferes with ribosome binding, which in turn results in rapid degradation of the transcript. In contrast, Hfq is also required for efficient translation of the E. coli and Salmonella typhimurium rpoS gene, encoding the stationary sigma factor. In this study, the authors have isolated and characterized the Hfq homologue of Pseudomonas aeruginosa (Hfq(Pa)), which consists of only 82 aa. The 68 N-terminal amino acids of Hfq(Pa) show 92% identity with Hfq(Ec). Hfq(Pa) was shown to functionally replace Hfq(Ec) in terms of its requirement for phage Qbeta replication and for rpoS expression. In addition, Hfq(Pa) exerted the same negative effect on E. coli ompA mRNA expression. As judged by proteome analysis, the expression of either the plasmid-borne hfq(Pa) or the hfq(Ec) gene in an E. coli Hfq(-) RpoS(-) strain revealed no gross difference in the protein profile. Both Hfq(Ec) and Hfq(Pa) affected the synthesis of approximately 26 RpoS-independent E. coli gene products. These studies showed that the functional domain of Hfq resides within its N-terminal domain. The observation that a C-terminally truncated Hfq(Ec) lacking the last 27 aa [Hfq(Ec(75))] can also functionally replace the full-length E. coli protein lends further support to this notion.  (+info)

The lysis function of RNA bacteriophage Qbeta is mediated by the maturation (A2) protein. (6/58)

Complete or partial cDNA sequences of the RNA bacteriophage Qbeta were cloned in plasmids under the control of the lambdaP(L) promoter to allow regulated expression in Escherichia coli harbouring the gene for the temperature-sensitive lambdaCI857 repressor. Induction of the complete Qbeta sequence leads to a 100-fold increase in phage production, accompanied by cell lysis. Induction of the 5'-terminal sequence containing the intact maturation protein (A2) cistron also causes cell lysis. Alterations of the A2 cistron, leading to proteins either devoid of approximately 20% of the C-terminal region or of six internal amino acids, abolish the lysis function. Expression of other cistrons in addition to the A2 cistron does not enhance host lysis. Thus, in Qbeta, the A2 protein, in addition to its functions as maturation protein, appears to trigger cell lysis. This contrasts with the situation in the distantly related group I RNA phages such as f2 and MS2 where a small lysis polypeptide is coded for by a region overlapping the end of the coat gene and the beginning of the replicase gene.  (+info)

Evolution of bacteriophage in continuous culture: a model system to test antiviral gene therapies for the emergence of phage escape mutants. (7/58)

The emergence of viral escape mutants is usually a highly undesirable phenomenon. This phenomenon is frequently observed in antiviral drug applications for the treatment of viral infections and can undermine long-term therapeutic success. Here, we propose a strategy for evaluating a given antiviral approach in terms of its potential to provoke the appearance of resistant virus mutants. By use of Q beta RNA phage as a model system, the effect of an antiviral gene therapy, i.e., a virus-specific repressor protein expressed by a recombinant Escherichia coli host, was studied over the course of more than 100 generations. In 13 experiments carried out in parallel, 12 phage populations became resistant and 1 became extinct. Sequence analysis revealed that only two distinct phage mutants emerged in the 12 surviving phage populations. For both escape mutants, sequence variations located in the repressor binding site of the viral genomic RNA, which decrease affinity for the repressor protein, conferred resistance to translational repression. The results clearly suggest the feasibility of the proposed strategy for the evaluation of antiviral approaches in terms of their potential to allow resistant mutants to appear. In addition, the strategy proved to be a valuable tool for observing virus-specific molecular targets under the impact of antiviral drugs.  (+info)

Qbeta replicase discriminates between legitimate and illegitimate templates by having different mechanisms of initiation. (8/58)

Qbeta replicase (RNA-directed RNA polymerase of bacteriophage Qbeta) exponentially amplifies certain RNAs (RQ RNAs) in vitro. Here we characterize template properties of the 5' and 3' fragments obtained by cleaving one of such RNAs at an internal site. We unexpectedly found that, besides the 3' fragment, Qbeta replicase can copy the 5' fragment and a number of its variants, although they lack the initiator region of RQ RNA. This copying can occur as a 3'-terminal elongation or through de novo initiation. In contradistinction to RQ RNA and its 3' fragment, initiation on these templates occurs without regard to the 3'-terminal or internal oligo(C) clusters, is GTP-independent, and does not result in a stable replicative complex capable of elongation in the presence of aurintricarboxylic acid. The results suggest that, although Qbeta replicase can initiate and elongate on a variety of RNAs, only some of them are recognized as legitimate templates. GTP-dependent initiation on a legitimate template drives the enzyme to a "closed" conformation that may be important for keeping the template and the complementary nascent strand unannealed, without which the exponential replication is impossible. Triggering the GTP-dependent conformational transition at the initiation step could serve as a discriminative feature of legitimate templates providing for the high template specificity of Qbeta replicase.  (+info)

*Allolevivirus

... is a genus of viruses, in the family Leviviridae. Enterobacteria serve as natural hosts. There are currently only ... Group: ssRNA(+) Order: Unassigned Family: Leviviridae Genus: Allolevivirus Enterobacteria phage FI Enterobacteria phage Qbeta ... Viruses in Allolevivirus are non-enveloped, with icosahedral and Spherical geometries, and T=3 symmetry. The diameter is around ...

*Taxonomic list of viruses

Allolevivirus Enterobacteria phage FI Enterobacteria phage Qbeta Genus: Levivirus Enterobacteria phage BZ13 Enterobacteria ...

*Leviviridae

Group: ssRNA(+) Order: Unassigned Family: Leviviridae Genus: Allolevivirus Enterobacteria phage FI Enterobacteria phage Qbeta ...

*List of MeSH codes (B04)

... allolevivirus MeSH B04.123.205.600.500 --- levivirus MeSH B04.123.205.891 --- t-phages MeSH B04.123.205.891.100 --- ... allolevivirus MeSH B04.123.691.600.500 --- levivirus MeSH B04.123.706.070 --- bacteriophage p22 MeSH B04.123.900.150 --- ... allolevivirus MeSH B04.820.410.500 --- levivirus MeSH B04.820.455.149 --- bornaviridae MeSH B04.820.455.149.135 --- borna ... allolevivirus MeSH B04.123.450.500 --- levivirus MeSH B04.123.470.500 --- microvirus MeSH B04.123.470.500.320 --- bacteriophage ...

*List of genera of viruses

Ahjdlikevirus Alfamovirus Allexivirus Allolevivirus Alphabaculovirus Alphacarmotetravirus Alphacoronavirus Alphaentomopoxvirus ...
Page contains details about virus-like nanoparticles . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
Allolevivirus is a genus of viruses, in the family Leviviridae. Enterobacteria serve as natural hosts. There are currently only two species in this genus including the type species Enterobacteria phage Qbeta. Group: ssRNA(+) Order: Unassigned Family: Leviviridae Genus: Allolevivirus Enterobacteria phage FI Enterobacteria phage Qbeta Viruses in Allolevivirus are non-enveloped, with icosahedral and Spherical geometries, and T=3 symmetry. The diameter is around 26 nm. Genomes are linear and non-segmented, around 4kb in length. The genome codes for 4 proteins. Entry into the host cell is achieved by adsorption into the host cell. Replication follows the positive stranded RNA virus replication model. Positive stranded rna virus transcription is the method of transcription. Translation takes place by suppression of termination. The virus exits the host cell by bacteria lysis. Enterobacteria serve as the natural host. "Viral Zone". ExPASy. Retrieved 15 June 2015. ICTV. "Virus Taxonomy: 2014 Release". ...
In the present study, we describe a therapy for cat allergy based on immunization with the recombinant cat allergen Fel d1 displayed on VLPs derived from the bacteriophage Qβ (Qβ-Fel d1). The therapy is characterized by three key features: (a) Qβ-Fel d1 is highly immunogenic and a single vaccination is sufficient for therapy; (b) Qβ-Fel d1 is essentially nonreactogenic; and (c) the effector mechanism of the therapy is based upon the induction of allergen-specific IgGs.. Qβ-VLPs consist of 180 subunits of a 14-kD coat protein. These VLPs elicit strong B cell responses as a result of their highly organized and repetitive structures (Jegerlehner et al., 2002a,b). This feature can be exploited to enhance the immunogenicity of self- and foreign antigens. Chemical coupling of antigens via a cysteine to surface lysine residues on the VLP renders these antigens equally repetitive and consequently immunogenic. Moreover, host cell RNA, a ligand for TLR3 and TLR7 (Kanzler et al., 2007), is ...
JPT Peptide Technologies is a DIN ISO 9001:2015 certified and GCLP compliant integrated provider of innovative peptide based catalog products and custom services.
Miranda Castro QSS: Pathology Set - Pathology Set: $215 (save $10). Price includes 357 Pathology Cards, 6 CDs and study guidelines. And shipping: use FREESHIP40 on checkout. The Quick Study System by Gwynn Cadwallader is a beautiful learning tool specifically created to help homeopathic students, study groups and homeopaths prepare for the certification examination of the Council for
Rich graphical models for real-world scene understanding encode the shape and pose of objects via high-dimensional, continuous variables. We describe a particle-based max-product inference algorithm which maintains a diverse set of posterior mode hypotheses, and is robust to initialization. At each iteration, the set of particle hypotheses is augmented via stochastic proposals, and then reduced via an optimization algorithm that minimizes distortions in max-product messages. Our particle selection metric is submodular, and thus efficient greedy algorithms have rigorous optimality guarantees. By avoiding the stochastic resampling steps underlying standard particle filters, we also avoid common degeneracies where particles collapse onto a single hypothesis. Our approach significantly outperforms previous particle-based algorithms in the estimation of human pose from images and videos, and the prediction of protein side-chain conformations ...
1. AAN QSS Init. Treatment PD (Jan 2002) Miyasaki JM, Martin W, Suchowersky O, Weiner WJ, Lang AE. Practice parameter: initiation of treatment for Parkinsons disease: an evidence-based review: Report of the Quality Standards Subcommittee of the American Academy of Neurology. Neurology 2002 Jan 8; 58(1):11-7. 2. AAN QSS PD Diag. (April 2006) Suchowersky O, Reich S, Perlmutter J, Zesiewicz T, Gronseth G, Weiner WJ, Quality Standards Subcommittee of the American Academy of Neurology. Practice parameter: diagnosis and prognosis of new onset Parkinson disease (an evidence-based review): report of the Quality Standards Subcommittee of the American Academy of Neurology. Neurology 2006 Apr 11; 66(7):968-75. 3. AAN QSS PD Dyskin (April 2006) Pahwa R, Factor SA, Lyons KE, Ondo WF, Gronseth G, Bronte-Stewart H, Hallet M, Miyasaki J, Stevens J, and Weiner WJ. Practice Parameter: Treatment of Parkinsons disease with motor fluctuations and dyskinesia (an evidence-based review): Report of the quality ...
Mesothelin is found in other cancers for several years," said Yao, also a researcher in the Dan L. Duncan Cancer Center at BCM. "However, we didnt know the role it played in pancreatic cancer:" until she and her colleagues reported in this article. In fact, they found very high levels of mesothelin in 18 of 21 samples of patients pancreatic tissues compared to amounts found in nearby normal tissues. In studies of this protein in the lab, pancreatic cancer cell lines that produced high levels of mesothelin grew faster and spread more than those in which mesothelin levels were lower. Pancreatic cancer cells grew and spread faster in mice whose tumors expressed high levels of mesothelin than in those whose cancer did not, said the researchers, who conducted the studies in an immune deficient mouse. ...
Talking to the Misinformed: How Politicians Communicate with Constituents Who Lack Accurate Information.". "Party Polarization in Factual Beliefs: Evidence from Government Officials and the Mass Public" (with Nathan Lee and Brendan Nyhan).. "Evaluating New Approaches to Promoting Vaccination: A Field Experiment on Parents in Vermont" (with Bridget Ahrens, Christine Finley, Shari Levine, and Brendan Nyhan).. "Combating Conspiracy Theories about Disease Epidemics: An Experiment on Zika in Brazil" (with Victoria Chi and Brendan Nyhan).. "Misperceptions, Corrections, and the Structure of Political Opinions" (with Caitlin Davies).. "Decider in Chief? Public Misperceptions about Presidential Power" (with Scott Clifford and Brendan Nyhan).. "Factual (In)accuracy and Partisan Selective Exposure" (with students in my QSS 30.08 seminar).. ...
Novavax is developing vaccines based on virus-like particle (VLP) technology developed at the University of Massachusetts Medical School (UMMS) in Worcester.
Novavax is developing vaccines based on virus-like particle (VLP) technology developed at the University of Massachusetts Medical School (UMMS) in Worcester.
Content in the Dryad Digital Repository is offered "as is." By downloading files, you agree to the Dryad Terms of Service. To the extent possible under law, the authors have waived all copyright and related or neighboring rights to this data. ...
A crack in a high mineral-content material, like bone or a synthetic mineral/polymeric composite, generates ion gradients which can be utilized for active targeting and repair. Employing is technique, an active self-propelled particle-based detection, delivery, and repair strategy for cracks is designed by utilizing the damaged matrix itself as both the trigger and the fuel. Our approach augments current research methods focused on promoting bone healing by delivery of a therapeutic agent to the bone via passive diffusion.
Gene expression is typically regulated by gene regulatory proteins that bind to the DNA. Experiments have shown that these proteins find their DNA target site via a combination of 3D diffusion in the cytoplasm and 1D diffusion along the DNA. This stochastic transport sets a fundamental limit on the precision of gene regulation. We derive this limit analytically and show by particle-based GFRD simulations that our expression is highly accurate under biologically relevant conditions ...
Learn BCC AVX inside-out with the help of Boris TV trainer Kevin McAuliffe. Featuring over 120 minutes of video, this learn-at-your-own-pace tutorial series includes all original materials, allowing you to follow along step-by-step. Only $9.99! Download it today. Now including: Wild Cards - New! Build complex designs of 2D images in 3D space with this powerful and yet easy-to-use particle-based filter.
To determine the prevalence of parvovirus 4 infection and its clinical and sociodemographic correlations in Finland, we used virus-like particle-based serodiagnostic procedures (immunoglobulin [Ig] G, IgM, and IgG avidity) and PCR. We found 2 persons with parvovirus 4 primary infection who had mild or asymptomatic clinical features among hepatitis C virus-infected injection drug users.
This thesis outlines adaptivity schemes for particle-based methods for the simulation of nearly incompressible fluid flows. As with the remeshing schemes used in mesh and grid-based methods, there is a need to use localized refinement in particle methods to reduce computational costs. Various forms of particle refinement have been proposed for particle-based methods such as Smoothed Particle Hydrodynamics (SPH). However, none of the techniques that exist currently are able to retain the original degree of randomness among particles. Existing methods reinitialize particle positions on a regular grid. Using such a method for region localized refinement can lead to discontinuities at the interfaces between refined and unrefined particle domains. In turn, this can produce inaccurate results or solution divergence. This thesis outlines the development of new localized refinement algorithms that are capable of retaining the initial randomness of the particles, thus eliminating transition zone ...
The presence of obstacles modifies the way in which particles diffuse. In cells it is observed that the mean-square displacement of biomolecules scales as a power law with exponent smaller than one. This behavior, called anomalous diffusion, is due to the presence of macromolecules playing the role of obstacles. We discuss the effect of fixed macroscopic obstacles on the time needed by particles to cross a strip and we consider both a diffusive and a ballistic regime. We find that in some regimes this residence time is not monotonic with respect to the size and the location of the obstacles. We discuss our results for particles performing random walks on a two dimensional strip considering also the effect of an exclusion rule. Results obtained in collaboration with A. Ciallella (Rome), O. Krehel (Eindhoven), A. Muntean (Karlstadt), R. van Santen (Eindhoven), and A. Sengar (Eindhoven) will be discussed.. ...
The Obama administration says it is concerned that some states are restricting access by low-income people to costly, revolutionary drugs for a liver-wasting disease called hepatitis C.
Why would they need lysogeny-or even, how could they lysogenize? I cant speak for them all, but MS2 is a (+)-stranded ssRNA phage. On infection, the RNA can be directly translated into proteins that replicate the phage RNA and direct the synthesis of any capsid proteins required to form new phage particles. The replicase enzyme makes copies of both plus and minus strands, though the latter are only used as a template to make more (+) strand for both translation and packaging into phage. To lysogenize, there needs to be a DNA phase to the replication cycle and there isnt any such phase for MS2 ...
Assume a particle-based fundamental physics. Then the non-living things in the universe outnumber the living by many orders of magnitude. But here is a striking fact given a restricted compositionality like van Inwagens, Toners or mine on which all there are is in the universe are particles and organisms: the number of kinds of living things outnumbers the number of kinds of non-living things by several orders of magnitude. The number of kinds of particles is of the order of 100, but there are millions of biological species (they may not all correspond to metaphysical species, of course).. Counting by individuals, living things are exceptional. But counting by kinds, physical things are exceptional. Only a tiny portion of the universe is occupied by life. But on the other hand, only a tiny portion of the space of kinds of entities is occupied by non-life.. I am not sure what to make of these observations. Maybe it is gives some credence to an Aristotelian rather than Humean way of seeing the ...
Circulating tumor cells (CTCs) are shed from solid tumors and found at extremely low frequencies in the blood of patients in most cancers. A subset of these cells can seed and give rise to metastases, which is the primary cause of cancer-related mortality. Isolation and characterization of these cells from blood as a liquid biopsy can be a sensitive, non-invasive method for early detection, disease monitoring and therapy selection. CTCs can be found even at early disease stages in preclinical models and patients. There is increasing evidence that clusters of CTCs in blood are associated with higher metastatic potential; however, efficient isolation and interrogation of these rare clusters is challenging. In this study, we utilize an in-line rare cell enrichment platform developed by Becton Dickinson (BD), coupled with the BD FACSTM Influx cell sorter to rapidly isolate both single cells and clusters from blood. This platform utilizes magnetic particle-based depletion of unwanted leukocytes and ...
MCell (Monte Carlo Cell) is a program for simulating spatially resolved cell models using particle-based Monte Carlo algorithms. Biological processes at the cell level take place in small and often...
from Genetic Engineering News by Michael Tackett, Graeme Doran, Daniel Pregibon Particle-Based Approach Profiles Up to 68 miRNAs from Plasma, Serum, and Exosomes MicroRNA (miRNA) profiling has tremendous potential for the diagnosis and prognosis of a broad range of diseases including cancer, cardiovascular disorders,
My late 2013 13 rMBP has a really annoying mura in the lower right quadrant of the display. The original display was replaced under warranty when the...
Assistant Professor. research • biography • lab members • publications • data and analysis tools. Biography:. Dennis Ko received a B.S. from Cornell University in 1997. His research career began in the lab of Dr. Jeffrey W. Roberts at Cornell University. Using a genetic screen of the bacterial initiation factor σ70 followed by biochemical characterization, he identified a surface on the protein important for transcriptional regulation by the phage Q protein and revealed a novel role for a sigma factor beyond transcription initiation.. His training continued from 1997-2005 in the MSTP at Stanford University. He completed both MD and PhD degrees but clearly saw that his future was in research aimed at understanding the biology of disease. His thesis project was carried out in the laboratory of Dr. Matthew P. Scott. Using a combination of biochemistry, cell biology, genetics and animal models, he pioneered a new subject in the lab examining how genetic alterations lead to the ...

Allolevivirus - WikipediaAllolevivirus - Wikipedia

Allolevivirus is a genus of viruses, in the family Leviviridae. Enterobacteria serve as natural hosts. There are currently only ... Group: ssRNA(+) Order: Unassigned Family: Leviviridae Genus: Allolevivirus Enterobacteria phage FI Enterobacteria phage Qbeta ... Viruses in Allolevivirus are non-enveloped, with icosahedral and Spherical geometries, and T=3 symmetry. The diameter is around ...
more infohttps://en.wikipedia.org/wiki/Allolevivirus

The Big Picture Book of Viruses - Baltimore ListingThe Big Picture Book of Viruses - Baltimore Listing

Allolevivirus. enterobacteria phage Q-B. Bacteria. Luteovirus:. barley yellow dwarf virus. Plants. ...
more infohttp://www.virology.net/Big_Virology/BVFamilyGroup.html

Escherichia phage Qbeta (Bacteriophage Q-beta)Escherichia phage Qbeta (Bacteriophage Q-beta)

Allolevivirus subgroup III. › Bacteriophage Q beta. › Bacteriophage Qbeta. › Enterobacteria allolevivirus I. › Enterobacteria ...
more infohttp://www.uniprot.org/taxonomy/39803

Viruses  | Free Full-Text | Comparative Analysis of 37 Acinetobacter Bacteriophages | HTMLViruses | Free Full-Text | Comparative Analysis of 37 Acinetobacter Bacteriophages | HTML

The Leviviridae are a family of small single-stranded RNA viruses currently separated into two genera; Allolevivirus and ...
more infohttps://www.mdpi.com/1999-4915/10/1/5/htm

Show GenusShow Genus

Notes on Genus: Allolevivirus. icosahedra. Type member: Enterobacteria phage Qb. Contents. Members of this Genus. General ...
more infohttp://dpvweb.net/notes/showgenus.php?genus=Allolevivirus

Leviviridae<...Leviviridae<...

Genus Allolevivirus. Type species Enterobacteria phage Qbeta. Distinguishing features. Alloleviviruses contain the longer ... List of other related viruses which may be members of the genus Allolevivirus but have not been approved as species. None ... Generally, the replicases from leviviruses poorly replicate allolevivirus RNA and vice versa. ... Antigenic specificity is distinct from that of members of the genus Allolevivirus. ...
more infohttps://talk.ictvonline.org/ictv-reports/ictv_9th_report/positive-sense-rna-viruses-2011/w/posrna_viruses/263/leviviridae

Search Articles | University of Toronto LibrariesSearch Articles | University of Toronto Libraries

DNA, Bacterial - isolation & purification , Methylene Blue - metabolism , Coloring Agents , Allolevivirus - physiology , ... Spectrometry, Fluorescence , Methylene Blue - pharmacology , Pyronine - metabolism , Allolevivirus - radiation effects , ...
more infohttps://query.library.utoronto.ca/index.php/search/q?kw=SubjectTerms:Pyronine%20-%20pharmacology

Zhang, Junjie - Department of Biochemistry and BiophysicsZhang, Junjie - Department of Biochemistry and Biophysics

Asymmetric cryo-EM structure of the canonical Allolevivirus Qβ reveals a single maturation protein and the genomic ssRNA in ...
more infohttps://biochemistry.tamu.edu/people/zhang-junjie/

Host Factor 1 Protein | Profiles RNSHost Factor 1 Protein | Profiles RNS

ALLOLEVIVIRUS). Its cellular function may be to regulate mRNA stability and processing in that it binds tightly to poly(A) RNA ...
more infohttps://profiles.umassmed.edu/display/106668

View source for Levivirus - microbewikiView source for Levivirus - microbewiki

... with Allolevivirus being the other. It replicates in only three bacteria genera: Escherichia, Pseudomonas, and Caulobacter. The ...
more infohttps://microbewiki.kenyon.edu/index.php?title=Levivirus&action=edit&oldid=72935

Multiple sequence alignments of partially coding nucleic acid sequences | BMC Bioinformatics | Full TextMultiple sequence alignments of partially coding nucleic acid sequences | BMC Bioinformatics | Full Text

This stem-loop-structure is well known and defined in Qβ (Allolevivirus).. ... Levivirus and Allolevivirus) are ssRNA positive-strand viruses. The replication cycle includes no DNA stage. The virions are ...
more infohttps://bmcbioinformatics.biomedcentral.com/articles/10.1186/1471-2105-6-160

Leviviridae - Figures<...Leviviridae - Figures<...

Figure 2 General genetic map of a representative levivirus - Enterobacteria phage MS2 (MS2) - and an allolevivirus - ...
more infohttps://talk.ictvonline.org/ictv-reports/ictv_9th_report/positive-sense-rna-viruses-2011/w/posrna_viruses/264/leviviridae-figures

Taxonomic list of viruses - WikipediaTaxonomic list of viruses - Wikipedia

Allolevivirus Enterobacteria phage FI Enterobacteria phage Qbeta Genus: Levivirus Enterobacteria phage BZ13 Enterobacteria ...
more infohttps://en.wikipedia.org/wiki/Taxonomic_list_of_viruses

PROCESS OF PRODUCTION OF BACTERIOPHAGE COMPOSITIONS AND METHODS IN PHAGE THERAPY FIELD - Patent applicationPROCESS OF PRODUCTION OF BACTERIOPHAGE COMPOSITIONS AND METHODS IN PHAGE THERAPY FIELD - Patent application

This family comprises allolevivirus and levivirus genus having no separate gene for cell lysis and a separate gene for cell ...
more infohttp://www.patentsencyclopedia.com/app/20090232770

Category:Low-importance virus articles - WikipediaCategory:Low-importance virus articles - Wikipedia

Talk:Allolevivirus. *Talk:Alphabaculovirus. *Talk:Alphaentomopoxvirus. *Talk:Alphaflexiviridae. *Talk:Alphafusellovirus. *Talk: ...
more infohttps://en.wikipedia.org/wiki/Category:Low-importance_virus_articles

Virus-Taxonomie - WikipediaVirus-Taxonomie - Wikipedia

Genus Allolevivirus, mit Species Escherichia virus Qbeta. *Genus Levivirus, mit Species Escherichia virus MS2 ...
more infohttps://de.wikipedia.org/wiki/Virus-Taxonomie

Purchase Brahmi. Cheap Brahmi OTC.Purchase Brahmi. Cheap Brahmi OTC.

Unlike were incubated with antisera from each phages from the genus Levivirus, Allolevivirus group and then grown on their ...
more infohttp://www.kelcommunique-de-presse.com/medical/clinical-trial-2/brahmi/

Genome size. Medical search. DefinitionsGenome size. Medical search. Definitions

Allolevivirus: A bacteriophage genus of the family LEVIVIRIDAE, whose viruses contain the longer version of the genome and have ... MycoplasmaArabidopsisMammalsGossypiumRotiferaAphidsRNA VirusesVertebratesAlligators and CrocodilesHelianthusAllolevivirus ...
more infohttps://lookformedical.com/en/definitions/genome-size

IRMNG  - AllolevivirusIRMNG - Allolevivirus

IRMNG (2018). Allolevivirus. Accessed at: http://www.irmng.org/aphia.php?p=taxdetails&id=1039803 on 2019-10-14 ...
more infohttp://www.irmng.org/aphia.php?p=taxdetails&id=1039803

Recode2 :: Database of translational recoding eventsRecode2 :: Database of translational recoding events

Allolevivirus. Methanocaldococcus jannaschii. Archaea (Chromosome). Selenocysteine. Validated. Methanocaldococcus. Drosophila ...
more infohttp://recode.ucc.ie/browse/status/Validated?page=0&order=4
  • Asymmetric cryo-EM structure of the canonical Allolevivirus Qβ reveals a single maturation protein and the genomic ssRNA in situ. (tamu.edu)