Alkanes
Alkane 1-Monooxygenase
Oxygenases
Mixed Function Oxygenases
Widely distributed enzymes that carry out oxidation-reduction reactions in which one atom of the oxygen molecule is incorporated into the organic substrate; the other oxygen atom is reduced and combined with hydrogen ions to form water. They are also known as monooxygenases or hydroxylases. These reactions require two substrates as reductants for each of the two oxygen atoms. There are different classes of monooxygenases depending on the type of hydrogen-providing cosubstrate (COENZYMES) required in the mixed-function oxidation.
Kynurenine 3-Monooxygenase
Methylococcaceae
Methylococcus capsulatus
Cytochrome P-450 Enzyme System
A superfamily of hundreds of closely related HEMEPROTEINS found throughout the phylogenetic spectrum, from animals, plants, fungi, to bacteria. They include numerous complex monooxygenases (MIXED FUNCTION OXYGENASES). In animals, these P-450 enzymes serve two major functions: (1) biosynthesis of steroids, fatty acids, and bile acids; (2) metabolism of endogenous and a wide variety of exogenous substrates, such as toxins and drugs (BIOTRANSFORMATION). They are classified, according to their sequence similarities rather than functions, into CYP gene families (>40% homology) and subfamilies (>59% homology). For example, enzymes from the CYP1, CYP2, and CYP3 gene families are responsible for most drug metabolism.
Biodegradation, Environmental
Pseudomonas
Petroleum
Oxidation-Reduction
A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).
Methylosinus trichosporium
Octanes
Camphor 5-Monooxygenase
A soluble cytochrome P-450 enzyme that catalyzes camphor monooxygenation in the presence of putidaredoxin, putidaredoxin reductase, and molecular oxygen. This enzyme, encoded by the CAMC gene also known as CYP101, has been crystallized from bacteria and the structure is well defined. Under anaerobic conditions, this enzyme reduces the polyhalogenated compounds bound at the camphor-binding site.
Rubredoxins
Alkenes
Methane
Hydroxylation
Nitrosomonas
Flavin-Adenine Dinucleotide
Pseudomonas putida
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Gordonia Bacterium
Benzydamine
Tyrosine 3-Monooxygenase
Squalene Monooxygenase
The second enzyme in the committed pathway for CHOLESTEROL biosynthesis, this enzyme catalyzes the first oxygenation step in the biosynthesis of STEROLS and is thought to be a rate limiting enzyme in this pathway. Specifically, this enzyme catalyzes the conversion of SQUALENE to (S)-squalene-2,3-epoxide.
Oxidoreductases
The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)
Substrate Specificity
FMN Reductase
NADPH-Ferrihemoprotein Reductase
Amidine-Lyases
Multienzyme Complexes
Flavins
Gram-Negative Aerobic Bacteria
Alcanivoraceae
Trichloroethylene
Microsomes, Liver
Methylocystaceae
Catalysis
Amino Acid Sequence
Hydrocarbons, Chlorinated
Waxes
A plastic substance deposited by insects or obtained from plants. Waxes are esters of various fatty acids with higher, usually monohydric alcohols. The wax of pharmacy is principally yellow wax (beeswax), the material of which honeycomb is made. It consists chiefly of cerotic acid and myricin and is used in making ointments, cerates, etc. (Dorland, 27th ed)
Beijerinckiaceae
Alcohols
Cloning, Molecular
NADP
Nicotinamide adenine dinucleotide phosphate. A coenzyme composed of ribosylnicotinamide 5'-phosphate (NMN) coupled by pyrophosphate linkage to the 5'-phosphate adenosine 2',5'-bisphosphate. It serves as an electron carrier in a number of reactions, being alternately oxidized (NADP+) and reduced (NADPH). (Dorland, 27th ed)
Oxidoreductases, O-Demethylating
7-Alkoxycoumarin O-Dealkylase
Pseudomonas mendocina
Acinetobacter
Biotransformation
The chemical alteration of an exogenous substance by or in a biological system. The alteration may inactivate the compound or it may result in the production of an active metabolite of an inactive parent compound. The alterations may be divided into METABOLIC DETOXICATION, PHASE I and METABOLIC DETOXICATION, PHASE II.
Acetic Anhydrides
Sequence Analysis, DNA
Dichloroethylenes
Oxygen
Hydrocarbons, Aromatic
Escherichia coli
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Multigene Family
A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)
Pseudomonas stutzeri
A species of gram-negative bacteria in the genus PSEUDOMONAS, containing multiple genomovars. It is distinguishable from other pseudomonad species by its ability to use MALTOSE and STARCH as sole carbon and energy sources. It can degrade ENVIRONMENTAL POLLUTANTS and has been used as a model organism to study denitrification.
Ammonia
Microsomes
Artifactual vesicles formed from the endoplasmic reticulum when cells are disrupted. They are isolated by differential centrifugation and are composed of three structural features: rough vesicles, smooth vesicles, and ribosomes. Numerous enzyme activities are associated with the microsomal fraction. (Glick, Glossary of Biochemistry and Molecular Biology, 1990; from Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Pseudomonas fluorescens
Acetone
Copper
Enzyme Induction
Chloroquinolinols
8-Hydroxyquinolinols chlorinated on the number 5 and/or 7 carbon atom(s). They are antibacterial, antiprotozoal, and antidiarrheal, especially in amebiasis, and have also been used as antiseborrheics. The compounds are mostly used topically, but have been used also as animal feed additives. They may cause optic and other neuropathies and are most frequently administered in combination with other agents.
Base Sequence
Chromatography, Gas
Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix.
Tryptophan Hydroxylase
Agrocybe
Epoxy Compounds
Molecular Structure
Iron
Glyceryl Ethers
Flavin Mononucleotide
4-Hydroxybenzoate-3-Monooxygenase
Models, Chemical
Rhodium
The PalkBFGHJKL promoter is under carbon catabolite repression control in Pseudomonas oleovorans but not in Escherichia coli alk+ recombinants. (1/260)
The alk genes are located on the OCT plasmid of Pseudomonas oleovorans and encode an inducible pathway for the utilization of n-alkanes as carbon and energy sources. We have investigated the influence of alternative carbon sources on the induction of this pathway in P. oleovorans and Escherichia coli alk+ recombinants. In doing so, we confirmed earlier reports that induction of alkane hydroxylase activity in pseudomonads is subject to carbon catabolite repression. Specifically, synthesis of the monooxygenase component AlkB is repressed at the transcriptional level. The alk genes have been cloned into plasmid pGEc47, which has a copy number of about 5 to 10 per cell in both E. coli and pseudomonads. Pseudomonas putida GPo12 is a P. oleovorans derivative cured of the OCT plasmid. Upon introduction of pGEc47 in this strain, carbon catabolite repression of alkane hydroxylase activity was reduced significantly. In cultures of recombinant E. coli HB101 and W3110 carrying pGEc47, induction of AlkB and transcription of the alkB gene were no longer subject to carbon catabolite repression. This suggests that carbon catabolite repression of alkane degradation is regulated differently in Pseudomonas and in E. coli strains. These results also indicate that PalkBFGHJKL, the Palk promoter, might be useful in attaining high expression levels of heterologous genes in E. coli grown on inexpensive carbon sources which normally trigger carbon catabolite repression of native expression systems in this host. (+info)Characterization of cytochrome P450 expression in human oesophageal mucosa. (2/260)
The expression of cytochrome (CYP) P450 enzymes in human oesophageal mucosa was investigated in a total of 25 histologically non-neoplastic surgical tissue specimens by using specific antibodies in immunoblots and by RT-PCR mRNA analysis. The presence of CYP1A, 2E1, 3A and 4A enzymes was demonstrated by both techniques; CYP2A reactive protein was also detected by immunoblot. The presence of CYP4B1 mRNA was established but no specific antibody was available for detection of the corresponding protein by immunoblot. CYP2B6/7 mRNA was not detected in any sample. The mRNA transcripts for CYP1A1, 2E1, 4A11 and 4B1 were consistently detected in the majority of samples (>84%), whereas CYP1A2 mRNA was only detected in 11 of 19 specimens examined. An RT-PCR method to differentiate CYP3A4 and 3A5 mRNA was developed. This demonstrated CYP3A5 mRNA expression in all samples tested, whereas CYP3A4 mRNA was not detectable, suggesting that CYP3A5 is the major CYP3A protein in human oesophagus. There were significant interindividual variations in the amount of proteins, ranging from 8-fold for CYP4A to 43-fold for CYP2E1. For each patient, data on exposure to risk factors for oesophageal cancer were available, including tobacco smoke, alcohol, gastro-oesophageal reflux and hot beverage consumption. None of these risk factors or other patient characteristics (age, sex, tumour location and tumour stage) were correlated with the protein level of the individual CYP enzymes as determined by quantitation of immunoblot staining. However, the small series of samples precludes any strong conclusion concerning the lack of such correlations. There were no differences between squamous cell carcinomas and adenocarcinomas in either the qualitative or quantitative expression of the CYP enzymes. These data demonstrate that a range of CYP enzymes are expressed in human oesophageal mucosa and indicate that this tissue has the capacity to activate chemical carcinogens to reactive DNA binding metabolites. (+info)Role of the alternative sigma factor sigmaS in expression of the AlkS regulator of the Pseudomonas oleovorans alkane degradation pathway. (3/260)
The AlkS protein activates transcription from the PalkB promoter, allowing the expression of a number of genes required for the assimilation of alkanes in Pseudomonas oleovorans. We have identified the promoter from which the alkS gene is transcribed, PalkS, and analyzed its expression under different conditions and genetic backgrounds. Transcription from PalkS was very low during the exponential phase of growth and increased considerably when cells reached the stationary phase. The PalkS -10 region was similar to the consensus described for promoters recognized by Escherichia coli RNA polymerase bound to the alternative sigma factor sigmaS, which directs the expression of many stationary-phase genes. Reporter strains containing PalkS-lacZ transcriptional fusions showed that PalkS promoter is very weakly expressed in a Pseudomonas putida strain bearing an inactivated allele of the gene coding for sigmaS, rpoS. When PalkS was transferred to E. coli, transcription started at the same site and expression was higher in stationary phase only if sigmaS-RNA polymerase was present. The low levels of AlkS protein generated in the absence of sigmaS were enough to support a partial induction of the PalkB promoter. The -10 and -35 regions of PalkS promoter also show some similarity to the consensus recognized by sigmaD-RNA polymerase, the primary form of RNA polymerase. We propose that in exponential phase PalkS is probably recognized both by sigmaD-RNA polymerase (inefficiently) and by sigmaS-RNA polymerase (present at low levels), leading to low-level expression of the alkS gene. sigmaS-RNA polymerase would be responsible for the high level of activity of PalkS observed in stationary phase. (+info)Insulin differentially affects xenobiotic-enhanced, cytochrome P-450 (CYP)2E1, CYP2B, CYP3A, and CYP4A expression in primary cultured rat hepatocytes. (4/260)
Uncontrolled diabetes results in enhanced expression of cytochrome P-450 (CYP)2E1, CYP2B, CYP3A, and CYP4A. Because of the simultaneous and confounding metabolic and hormonal changes that occur in vivo as a consequence of diabetes, primary cultured rat hepatocytes provide an excellent model system for examination of the effects of insulin on P-450 expression and on xenobiotic-mediated P-450 expression. In the present study, we examined the effects of insulin on pyridine-, phenobarbital-, and ciprofibrate-mediated expression of CYP2E1, CYP2B, CYP3A, and CYP4A in primary cultured rat hepatocytes. Pyridine addition to primary rat hepatocytes cultured in the presence of 1 nM insulin or in the absence of insulin resulted in a 3.5-fold and 3-fold enhancement in CYP2E1 protein expression, respectively, in the absence of any pyridine-mediated increase in mRNA expression. In contrast, hepatocytes cultured in the standard concentration of 1 microM insulin resulted in only a 2-fold increase in protein expression. Thus, the fold-induction of CYP2E1 protein in response to pyridine was 1.5- to 1.8-fold greater in either the absence of insulin or in the presence of 1 nM insulin, respectively, than that monitored in the presence of 1 microM insulin. To examine whether insulin effects on xenobiotic-mediated CYP2E1 expression were selective, insulin effects on xenobiotic-mediated expression of transcriptionally regulated CYP2B, CYP3A, and CYP4A were examined. Pyridine- or phenobarbital-mediated induction of CYP2B mRNA and protein expression in hepatocytes was suppressed by as much as 80% at lower insulin levels (0 and 1 nM), relative to the level monitored in the presence of 1 microM insulin. Omitting insulin from the medium resulted in a 50% decrease in CYP3A mRNA levels in response to phenobarbital treatment and a 30% decrease in CYP4A mRNA levels in response to ciprofibrate treatment, relative to the level obtained in response to these treatments in the presence of 1 microM insulin. The results of this study demonstrate that decreasing the insulin level in the primary hepatocyte culture medium enhanced xenobiotic-mediated CYP2E1 expression, whereas lower insulin levels suppressed xenobiotic-mediated CYP2B, CYP3A, and CYP4A expression in this cell culture system. (+info)Hypoxia-induced production of 12-hydroxyeicosanoids in the corneal epithelium: involvement of a cytochrome P-4504B1 isoform. (5/260)
The corneal epithelium metabolizes arachidonic acid by a cytochrome P-450 (CYP)-mediated activity to 12-hydroxy-5,8,11, 14-eicosatetraenoic acid (12(R)-HETE) and 12-hydroxy-5,8, 14-eicosatrienoic acid (12(R)-HETrE ). Both metabolites possess potent inflammatory properties, with 12(R)-HETrE being a powerful angiogenic factor, and they assume the role of inflammatory mediators in hypoxia- and chemical-induced injury in the cornea in vivo and in vitro. We used a model of corneal organ culture that exhibits hypoxia-induced epithelial CYP-dependent 12(R)-HETE and 12(R)-HETrE synthesis for isolating, identifying, and characterizing the CYP protein responsible for these eicosanoid syntheses. Northern analysis revealed the presence of a CYP4A-hybridizable mRNA, the levels of which were increased after hypoxia. Reverse transcription-polymerase chain reaction analysis with primers specific for the CYP4A family led to the isolation of a 671-base pair fragment with a 98.8% sequence homology to the rabbit lung CYP4B1 isoform, of which the levels in the corneal epithelium were greatly increased under hypoxic conditions. Moreover, phenobarbital, an inducer of hepatic CYP4B1 in the rabbit, also induced 12-HETE and 12-HETrE synthesis. Antibodies against CYP4B1, but not against CYP4A1, inhibited hypoxia-, clofibrate-, and phenobarbital-induced 12-HETE and 12-HETrE synthesis. These results suggest the involvement of a CYP4B1 isoform in the corneal epithelial synthesis of these eicosanoids in response to hypoxia. (+info)Arachidonic acid and PGE2 regulation of hepatic lipogenic gene expression. (6/260)
N-6 polyunsaturated fatty acids (PUFA) suppress hepatic and adipocyte de novo lipogenesis by inhibiting the transcription of genes encoding key lipogenic proteins. In cultured 3T3-L1 adipocytes, arachidonic acid (20:4,n-6) suppression of lipogenic gene expression requires cyclooxygenase (COX) activity. In this study, we found no evidence to support a role for COX-1 or -2 in the 20:4,n-6 inhibition of hepatocyte lipogenic gene expression. In contrast to L1 preadipocytes, adipocytes and rat liver, RT-PCR and Western analyses did not detect COX-1 or COX-2 expression in cultured primary hepatocytes. Moreover, the COX inhibitor, flurbiprofen, did not affect the 20:4,n-6 regulation of lipogenic gene expression in primary hepatocytes. Despite the absence of COX-1 and -2 expression in primary hepatocytes, prostaglandins (PGE2 and PGF2alpha) suppressed fatty acid synthase, l-pyruvate kinase, and the S14 protein mRNA, while having no effect on acyl-CoA oxidase or CYP4A2 mRNA. Using PGE2 receptor agonist, the PGE2 effect on lipogenic gene expression was linked to EP3 receptors. PGE2 inhibited S14CAT activity in transfected primary hepatocytes and targeted the S14 PUFA-response region located -220 to -80 bp upstream from the transcription start site. Taken together, these studies show that COX-1 and COX-2 do not contribute to the n-6 PUFA suppression of hepatocyte lipogenic gene expression. However, cyclooxygenase products from non-parenchymal cells can act on parenchymal cells through a paracrine process and mimic the effects of n-6 PUFA on lipogenic gene expression. (+info)Kinetic profile of the rat CYP4A isoforms: arachidonic acid metabolism and isoform-specific inhibitors. (7/260)
20-Hydroxyeicosatetraenoic acid (HETE), the cytochrome P-450 (CYP) 4A omega-hydroxylation product of arachidonic acid, has potent biological effects on renal tubular and vascular functions and on the control of arterial pressure. We have expressed high levels of the rat CYP4A1, -4A2, -4A3, and -4A8 cDNAs, using baculovirus and Sf 9 insect cells. Arachidonic acid omega- and omega-1-hydroxylations were catalyzed by three of the CYP4A isoforms; the highest catalytic efficiency of 947 nM-1. min-1 for CYP4A1 was followed by 72 and 22 nM-1. min-1 for CYP4A2 and CYP4A3, respectively. CYP4A2 and CYP4A3 exhibited an additional arachidonate 11,12-epoxidation activity, whereas CYP4A1 operated solely as an omega-hydroxylase. CYP4A8 did not catalyze arachidonic or linoleic acid but did have a detectable lauric acid omega-hydroxylation activity. The inhibitory activity of various acetylenic and olefinic fatty acid analogs revealed differences and indicated isoform-specific inhibition. These studies suggest that CYP4A1, despite its low expression in extrahepatic tissues, may constitute the major source of 20-HETE synthesis. Moreover, the ability of CYP4A2 and -4A3 to catalyze the formation of two opposing biologically active metabolites, 20-HETE and 11, 12-epoxyeicosatrienoic acid, may be of great significance to the regulation of vascular tone. (+info)Regulation of P-450 4A activity in the glomerulus of the rat. (8/260)
We recently reported that an enzyme of the cytochrome P-450 4A family is expressed in the glomerulus, but there is no evidence that 20-hydroxyeicosatetraenoic acid (20-HETE) can be produced by this tissue. The purpose of present study was to determine whether glomeruli isolated from the kidney of rats can produce 20-HETE and whether the production of this metabolite is regulated by nitric oxide (NO) and dietary salt intake. Isolated glomeruli produced 20-HETE, dihydroxyeicosatrienoic acids, and 12-hydroxyeicosatetraenoic acid (4.13 +/- 0.38, 4.20 +/- 0.38, and 2. 10 +/- 0.20 pmol. min-1. mg protein-1, respectively) when incubated with arachidonic acid (10 microM). The formation of 20-HETE was dependent on the availability of NADPH and the PO2 of the incubation medium. The formation of 20-HETE was inhibited by NO donors in a concentration-dependent manner. The production of 20-HETE was greater in glomeruli isolated from the kidneys of rats fed a low-salt diet than in kidneys of rats fed a high-salt diet (5.67 +/- 0.32 vs. 2.83 +/- 0.32 pmol. min-1. mg protein-1). Immunoblot experiments indicated that the expression of P-450 4A protein in glomeruli from the kidneys of rats fed a low-salt diet was sixfold higher than in kidneys of rats fed a high-salt diet. These results indicate that arachidonic acid is primarily metabolized to 20-HETE and dihydroxyeicosatrienoic acids in glomeruli and that glomerular P-450 activity is modulated by NO and dietary salt intake. (+info)
The catalytic site of rat hepatic lauric acid omega-hydroxylase: Protein versus prosthetic heme alkylation in the omega...
Renal Cytochrome P450 Oxygenases and Preglomerular Vascular Response to Arachidonic Acid and Endothelin-1 Following Ischemia...
PGEC Web Page for Genome Sequencing
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JNHE01000002 gene2369 protein (Pseudomonas oleovorans) - STRING interaction network
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Alkane 1-monooxygenase
... alkane monooxygenase, 1-hydroxylase, AlkB, and alkane hydroxylase. It contains a diiron non-heme active site. McKenna EJ, Coon ... In enzymology, an alkane 1-monooxygenase (EC 1.14.15.3) is an enzyme that catalyzes the chemical reactions an alkane + reduced ... Alkanes of 6 to 22 carbons have been observed as substrates. This enzyme belongs to the family of oxidoreductases, specifically ... The systematic name of this enzyme class is alkane, reduced-rubredoxin:oxygen 1-oxidoreductase. Other names in common use ...
List of MeSH codes (D08)
... camphor 5-monooxygenase MeSH D08.811.682.690.708.170.500 - alkane 1-monooxygenase MeSH D08.811.682.690.708.170.915 - steroid ... 4-hydroxybenzoate 3-monooxygenase MeSH D08.811.682.690.708.557 - kynurenine 3-monooxygenase MeSH D08.811.682.690.708.601 - ... trans-cinnamate 4-monooxygenase MeSH D08.622.509.700 - pepsinogen a MeSH D08.622.509.725 - pepsinogen c MeSH D08.622.610.500 - ... monophenol monooxygenase MeSH D08.811.682.690.708.170 - cytochrome p-450 enzyme system MeSH D08.811.682.690.708.170.040 - aryl ...
Rubredoxin
... alkane,reduced-rubredoxin:oxygen 1-oxidoreductase) octane + reduced rubredoxin + O2 = 1-octanol + oxidized rubredoxin + H2O EC ... 2-monooxygenase [(+)-camphor,reduced-rubredoxin:oxygen oxidoreductase (1,2-lactonizing)] (+)-bornane-2,5-dione + reduced ... rubredoxin + O2 = 5-oxo-1,2-campholide + oxidized rubredoxin + H2O EC 1.14.15.3 alkane 1-monooxygenase ( ...
Sphingobacterium olei
... olei to utilize petroleum hydrocarbons in farmland soil can be explained by its lack of alkane monooxygenase and aromatic ring- ... 1-13. ISBN 978-1-118-96060-8. Yabuuchi, E.; T. Kaneko; I. Yano; C.W. Moss; N. Miyoshi. (1 July 1983). "Sphingobacterium gen. ... Liu, Bin; Yang, Xiaojun; Sheng, Mengyao; Yang, Zhou; Qiu, Jiguo; Wang, Chenghong; He, Jian (1 March 2020). "Sphingobacterium ... 1 June 2017). "Sphingobacterium alkalisoli sp. nov., isolated from a saline-alkaline soil". International Journal of Systematic ...
Ammonia monooxygenase
Rasche ME, Hicks RE, Hyman MR, Arp DJ (September 1990). "Oxidation of monohalogenated ethanes and n-chlorinated alkanes by ... "Evidence that particulate methane monooxygenase and ammonia monooxygenase may be evolutionarily related". FEMS Microbiology ... Ammonia monooxygenase (EC 1.14.99.39, AMO) is an enzyme, which catalyses the following chemical reaction ammonia + AH2 + O2 ... Ammonia+monooxygenase at the US National Library of Medicine Medical Subject Headings (MeSH) Biology portal. ...
Harold Basch
Musaev, Djamaladdin G.; Basch, Harold; Morokuma, Keiji (2002). "Theoretical Study of the Mechanism of Alkane Hydroxylation and ... "Computational studies of reaction mechanisms of methane monooxygenase and ribonucleotide reductase". Journal of Computational ... 23 (1): 59-76. doi:10.1002/jcc.1157. ISSN 0192-8651. PMID 11913390. Basch, Harold; Ratner, Mark A. (2003). "Binding at molecule ...
Desulfatibacillum alkenivorans AK-01
It has also been shown that AK-01 utilizes not only alkanes but also 1-alkenes, 1-alkanols, fatty acids and other organic acids ... but serves as a reactant in the hydroxylation of both aliphatic and aromatic hydrocarbons via monooxygenase and dioxygenase ... AK-01 is a delta-proteobacterium capable of utilizing C13-C18 alkanes as growth substrates (So et al., 1999). Analysis of ... Heat and pressure lead to the formation of a wide variety of hydrocarbons, including alkanes, alkenes, and cyclic/polycyclic ...
Methane monooxygenase
... (MMO) is an enzyme capable of oxidizing the C-H bond in methane as well as other alkanes. Methane ... The particulate methane monooxygenase and related ammonia monooxygenase are integral membrane proteins, occurring in ... "Evidence that particulate methane monooxygenase and ammonia monooxygenase may be evolutionarily related". FEMS Microbiol. Lett ... This is a classic monooxygenase reaction in which two reducing equivalents from NAD(P)H are utilized to split the O-O bond of ...
Shilov system
The transformation can also performed biologically by methane monooxygenase. Overall Transformation RH + H2O + [PtCl6]2− → ROH ... "Reactions of alkanes in solutions of platinum chloride complexes" Zh. Fiz. Khim. 1972, 46, 1353-13544 Gol'dshleger, N. F.; ... and the nucleophilic oxidation of the alkane substrate. An equivalent transformation is performed industrially by steam ... 1] and [2] ). Such premature oxidation shuts down the catalysis. Finally the PtIV-CH2R undergoes nucleophilic attack by OH− or ...
Alcanivorax pacificus
These monooxygenases are especially important, as they account for long-chain n-alkane hydroxylation. A. Pacificus is closely ... Found were genes encoding for four integral-membrane alkane monooxygenases, three cytochrome P450 enzymes, and four genes ... Part of this difference results from a 549 nucleootide fragment of the alkane hydroxylase gene alkB, which was amplified from ... Lai Q, Shao Z (December 2012). "Genome sequence of an alkane-degrading bacterium, Alcanivorax pacificus type strain W11-5, ...
Cometabolism
Another example is Mycobacterium vaccae, which uses an alkane monooxygenase enzyme to oxidize propane. Accidentally, this ... OX1 can degrade PCE under aerobic conditions by using toluene-o-xylene monooxygenase (ToMO), an enzyme they produce to derive ... Several aerobic microorganisms have been demonstrated to be capable of doing this, including n-alkane, aromatic compound (e.g. ... These bacteria degrade their growth-substrate methane with the enzyme methane monooxygenase (MMO). MMO was discovered to be ...
Gas to liquids
The methanol reacts in the presence of a zeolite catalyst to form alkanes. In terms of mechanism, methanol is partially ... The relevant enzymes are methane monooxygenases, which are found both in soluble and particulate (i.e. membrane-bound) ... alkanes), aromatics, naphthenes (cycloalkanes) and small amounts of olefins (alkenes), mostly from C 6 (number of carbon atoms ... Most of the non-condensed gas from the product separator becomes recycled gas and is sent back to the feed stream to Reactor 1 ...
Alcanivorax
The aerobic metabolism of alkanes is carried out through the terminal alkane oxidation pathway, where monooxygenases initiate ... borkumensis can consume a wider variety of alkanes than other known species. A. borkumensis primarily uses alkanes as its ... The A. borkumensis genome has many sequences that each code for a different type of alkane, allowing it to be highly adaptable ... Whereas most organisms use sugars or amino acids for their source of carbon/energy, A. borkumensis uses alkanes, a type of ...
Mycoremediation
Averaging across all studied species, 98.1%, 48.6%, and 76.4% of the initial Bunker C C10 alkane, C14 alkane, and phenanthrene ... certain fungi possess intracellular networks which constitute the xenome, consisting of cytochrome (CYP) P450 monooxygenases ... The subphylum Saccharomycotina mostly consists of yeasts and includes degraders of n-alkanes, n-alkylbenzenes, crude oil, the ... 198 (Pt 2): 1-11. doi:10.1016/j.jenvman.2017.05.010. PMID 28499155. The levels of adsorption of the phenolic and PAHs were ...
Extremophile
Thermophilic Thermus and Bacillus species have demonstrated higher gene expression for the alkane mono-oxygenase alkB at ... 5 (1): 109-16. doi:10.1111/j.1758-2229.2012.00348.x. PMID 23757139. Krupovic M, Gonnet M, Hania WB, Forterre P, Erauso G (2013 ... 26 (1): 44-71. doi:10.1039/b800164m. PMID 19374122. Rossi M, Ciaramella M, Cannio R, Pisani FM, Moracci M, Bartolucci S (July ... 4 (1): 110-121. Fakayode, S.O. (2005). "Impact assessment of industrial effluent on water quality of the receiving Alaro River ...
Methylobacillus flagellatus
... or methane monooxygenase (MMO), which degrade various environmental pollutants (i.e.: alkanes, alkenes, and mono- and poly- ... encoding a subunit of particulate methane monooxygenase), and fae (encoding formaldehyde activating enzyme) genes." The data ... 1. p. 233-238. Baev M V, Chistoserdova L V, Polanuer B M, et al. "Effect of formaldehyde on growth of obligate methylotroph ... p. 1-10. Marchenko GN, Marchenko ND, Tsygankov YD, Chistoserdov AY. "Organization of threonine biosynthesis genes from the ...
Methane
The enzyme methane monooxygenase produces methanol from methane, but cannot be used for industrial-scale reactions. Some ... Hydrogen Cycle Industrial gas Lake Kivu (more general: limnic eruption) List of straight-chain alkanes Methanation Methane ... 57-58 McBride, James Michael (1999) "Development of systematic names for the simple alkanes". Chemistry Department, Yale ... It is a group-14 hydride and the simplest alkane, and is the main constituent of natural gas. The relative abundance of methane ...
Alkane 1-monooxygenase - Wikipedia
... alkane monooxygenase, 1-hydroxylase, AlkB, and alkane hydroxylase. It contains a diiron non-heme active site. McKenna EJ, Coon ... In enzymology, an alkane 1-monooxygenase (EC 1.14.15.3) is an enzyme that catalyzes the chemical reactions an alkane + reduced ... Alkanes of 6 to 22 carbons have been observed as substrates. This enzyme belongs to the family of oxidoreductases, specifically ... The systematic name of this enzyme class is alkane, reduced-rubredoxin:oxygen 1-oxidoreductase. Other names in common use ...
Alkane 1-monooxygenase: Definition with Alkane 1-monooxygenase Pictures and Photos
Definition of Alkane 1-monooxygenase with photos and pictures, translations, sample usage, and additional links for more ... alkane. alkane 1-monooxygenase (current term). alkane series. alkanediyl. alkanediyls. alkanes. alkanesulfonates. ... Medical Definition of Alkane 1-monooxygenase. 1. Alkb is an integral membrane protein component Registry number: EC 1.14.15.3 ... Alkane 1-monooxygenase Pictures. Click the following link to bring up a new window with an automated collection of images ...
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Springer Handbook of Enzymes | SpringerLink
Cyp4a10 - Cytochrome P450 4A10 - Mus musculus (Mouse) - Cyp4a10 gene & protein
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH- ... leukotriene-B4 20-monooxygenase activity Source: MGI. *monooxygenase activity Source: UniProtKB ,p>Traceable Author Statement,/ ... alkane 1-monooxygenase activity Source: MGI. *arachidonic acid epoxygenase activity Source: MGI ... Long-chain fatty acid omega-monooxygenase (EC:1.14.14.80By similarity. Manual assertion inferred from sequence similarity toi ...
CYP4A22 - Cytochrome P450 4A22 precursor - Homo sapiens (Human) - CYP4A22 gene & protein
... omega-1)-hydroxylation of various fatty acids such as laurate and palmitate. Shows no activity towards arachidonic acid and ... alkane 1-monooxygenase activity Source: GO_Central ,p>Inferred from Biological aspect of Ancestor,/p> ,p>A type of phylogenetic ... arachidonic acid monooxygenase activity Source: GO_CentralInferred from biological aspect of ancestori*. "Phylogenetic-based ... Long-chain fatty acid omega-monooxygenase (EC:1.14.14.80*Search proteins in UniProtKB for this EC number. ...
Team:TU Delft/Project/alkane-degradation/parts - 2010.igem.org
Crystal Structure of Long-Chain Alkane Monooxygenase (LadA) in Complex with Coenzyme FMN: Unveiling the Long-Chain Alkane ... To create the alkane degradation constructs a number of genes encoding for alkane degradation enzymes were synthesized and ... BBa_K398017 - Long-chain (C15-36) alkane conversion. For the first step in the long-chain alkane degradation pathway the ... BBa_K398014 - Medium-chain (C5-13) alkane conversion. Our medium-chain hydrocarbon degrading strain contains the alkane ...
Team:TU Delft/Project/alkane-degradation/parts - 2010.igem.org
Crystal Structure of Long-Chain Alkane Monooxygenase (LadA) in Complex with Coenzyme FMN: Unveiling the Long-Chain Alkane ... oxidizes (cyclo)alkanes to their respective (cyclo)alkanols by transferring one oxygen atom from molecular oxygen to the alkane ... To create the alkane degradation constructs a number of genes encoding for alkane degradation enzymes were synthesized and ... BBa_K398017 - Long-chain (C15-36) alkane conversion. For the first step in the long-chain alkane degradation pathway the ...
Burkholderia cepacia (Palleroni and Holmes) Yabuuchi et al. ATCC ®
Nucleotide (GenBank) : AJ293344 Burkholderia cepacia partial alkB gene for putative alkane 1-monooxygenase. ... Nucleotide (GenBank) : L36602 Rhizobium meliloti (clone Rhizo70-1) heat shock protein 70 (hsp70) gene, complete cds. ... Nucleotide (GenBank) : L36603 Pseudomonas cepacia (clone Psudom70-1) heat shock protein 70 (hsp70) gene, complete cds. ...
EAWAG-BBD: reaction, reacID# r0615
Search Medline for alkane 1-monooxygenase AND tert-Butyl alcohol. 2 citations on August 16, 2012. tert-Butyl alcohol , , O2 + ... RH2 alkane , / 1-monooxygenase ,/ 1.14.15.3 , Search GenBank, 684 hits on Apr. 03, 2012 Kyoto ,\ ExPASy , \ , H2O + R v 2- ... From tert-Butyl alcohol to 2-Methyl-2-hydroxy-1-propanol. Graphic of the reaction.. Medline reference Steffan RJ, McClay K, ... Methyl-2-hydroxy-1-propanol Display a pathway starting from this reaction. EAWAG-BBD Biotransformation rules in accord with ...
IMET 7370 Strain Passport - StrainInfo
Gordonia bronchialis DSM 43247 putative alkane 1-monooxygenase (alkB) gene, partial cds. DSM 43247 T ... Molecular detection and phylogenetic analysis of the alkane 1-monooxygenase gene from Gordonia spp ... 43 items found, displaying 1 to 25.[First/Prev] 1, 2 [Next/Last] ... 43 items found, displaying 1 to 25.[First/Prev] 1, 2 [Next/Last ... Gordonia bronchialis DSM 43247 catechol 1,2-dioxygenase (catA) gene, partial cds. DSM 43247 T ...
Involvement of an Alkane Hydroxylase System of Gordonia sp. Strain SoCg in Degradation of Solid n-Alkanes | Applied and...
... putida GPo1 and were shown to oxidize alkanes ranging from C10 to C16 (21). Four alkane monooxygenase homologs (two as part of ... and LadA from Geobacillus thermodenitrificans NG80-2 is the first long-chain n-alkane monooxygenase functional on alkanes in ... Two distinct monooxygenases for alkane oxidation in Nocardioides sp. strain CF8. Appl. Environ. Microbiol. 67:4992-4998. ... Involvement of an Alkane Hydroxylase System of Gordonia sp. Strain SoCg in Degradation of Solid n-Alkanes Luca Lo Piccolo, ...
cyp4a12a Protein, cytochrome P450, family 4, subfamily a, polypeptide 12a - Creative BioMart
alkane 1-monooxygenase activity. CYP4A12A; CYP4F18; CYP4F12; CYP4A14; CYP4A10; CYP4F2; CYP4A11. aromatase activity. CYP2J2; ... monooxygenase activity. CYP2AD6; CYP2X7; CYP2AD3; CYP3A5; CYP1A4; CYP26C1; CYP46A1.4; CYP2P6; CYP4F22; CYP27C1. ... cyp4a12a has several biochemical functions, for example, alkane 1-monooxygenase activity, aromatase activity, heme binding. ...
The complete genomic sequence of Nocardia farcinica IFM 10152 | PNAS
Regulation of the Alkane Hydroxylase CYP153 Gene in a Gram-Positive Alkane-Degrading Bacterium, Dietzia sp. Strain DQ12-45-1b ... and styrene monooxygenase (Nfa12190, Nfa32440, and Nfa32460). Of 103 oxygenases, 27 are putative cytochrome P450 monooxygenases ... Involvement of an Alkane Hydroxylase System of Gordonia sp. Strain SoCg in Degradation of Solid n-Alkanes ... Two Novel Alkane Hydroxylase-Rubredoxin Fusion Genes Isolated from a Dietzia Bacterium and the Functions of Fused Rubredoxin ...
Leukotriene-B(4) omega-hydroxylase elisa and antibody
20-monooxygenase 2).. CYP4F3: Cytochromes P450 are a group of heme-thiolate monooxygenases. This enzyme requires molecular ... Molecular Function: alkane 1-monooxygenase activity; arachidonic acid epoxygenase activity; leukotriene-B4 20-monooxygenase ... Cytochromes P450 are a group of heme-thiolate monooxygenases. This enzyme requires molecular oxygen and NADPH for the omega- ... EPS8-like 1 (EPS8L1) ELISA Kit. • DRD3 Antibody. • 3-nitrotyrosine (3-NT) ELISA Kit. • TRIM10 Antibody. more .... ...
Protocols and Video Articles Authored by Janet K. Jansson
In addition, alkane biodegradation genes are described for the first time in members of Planctomycetes. ... Prospecting Biotechnologically-Relevant Monooxygenases from Cold Sediment Metagenomes: An In Silico Approach Marine Drugs. Apr ... Jan, 2016 , Pubmed ID: 26547568 We aimed to gain insight into the alkane degradation potential of microbial communities from ... A total of 6178 sequences annotated as alkane-1-monooxygenases (EC 1.14.15.3) were retrieved from a shotgun metagenomic dataset ...
RCSB PDB - 3LNP: Crystal Structure of Amidohydrolase family Protein OLEI01672 1 465 from Oleispira antarctica
We identify an array of alkane monooxygenases, osmoprotectants, siderophores and micronutrient-scavenging pathways. We also ... Amidohydrolase family Protein OLEI01672_1_465. A. 468. Oleispira antarctica. Mutation(s): 0 Gene Names: mtaD. EC: 3.5.4.28 ( ... Crystal Structure of Amidohydrolase family Protein OLEI01672_1_465 from Oleispira antarctica. *DOI: 10.2210/pdb3LNP/pdb ...
István Máthé - ODT Personal data sheet
The detection and phylogenetic analysis of the alkane 1-monooxygenase gene of members of the genus Rhodococcus, SYSTEMATIC AND ... 1-7.. type of document: Journal paper/Article. number of independent citations: 2. language: English. DOI ... Part 1) pp. 298-301.. type of document: Journal paper/Article. number of independent citations: 10. language: English. DOI ... 1) pp. 87-98.. type of document: Journal paper/Article. number of independent citations: 6. language: English. DOI ...
Detection of Genes Involved in Biodegradation and Biotransformation in Microbial Communities by Using 50-Mer Oligonucleotide...
... and alkane monooxygenase) (3, 31, 34, 50). Thus, the developed microarray should be useful for assessing possible horizontal ... Bacterial strains, growth conditions, and soil samples.Pseudomonas putida Gpo1 (γ-proteobacterium; aerobic alkane degrader) and ... In addition, 4-chlorobenzate CoA ligase, alkane-1 monooxygenase, benzoyl CoA ligase, and cis-naphthalene dihydrodiol ... whereas very strong hybridization was observed for the alkane 1-monooxygenase gene (gi5824143) when the probe was 94% similar. ...
Code System Concept
Customized microscale approach for optimizing two-phase bio-oxidations of alkanes with high reproducibility | Microbial Cell...
... and chemoselective manner is efficiently catalyzed by monooxygenases such as the AlkBGT enzyme complex from Pseudomonas putida ... The direct ω-oxyfunctionalization of aliphatic alkanes in a regio- ... a robust high-throughput microwell platform for whole-cell two-liquid phase bio-oxidations of highly volatile n-alkanes. Using ... combining 1-dodecanol, dodecanal and dodecanoic acid) of up to 1.5 mmol g DCW −1 . Overall, the developed microwell plate ...
List of MeSH codes (D08) - Wikipedia
... camphor 5-monooxygenase MeSH D08.811.682.690.708.170.500 - alkane 1-monooxygenase MeSH D08.811.682.690.708.170.915 - steroid ... 4-hydroxybenzoate 3-monooxygenase MeSH D08.811.682.690.708.557 - kynurenine 3-monooxygenase MeSH D08.811.682.690.708.601 - ... trans-cinnamate 4-monooxygenase MeSH D08.622.509.700 - pepsinogen a MeSH D08.622.509.725 - pepsinogen c MeSH D08.622.610.500 - ... monophenol monooxygenase MeSH D08.811.682.690.708.170 - cytochrome p-450 enzyme system MeSH D08.811.682.690.708.170.040 - aryl ...
Prauserella soli sp. nov., isolated from crude oil-contaminated soil | Microbiology Society
Shen F.T., Young L.S., Hsieh M.F., Lin S.Y., Young C.C. ( 2010;). Molecular detection and phylogenetic analysis of the alkane 1 ... monooxygenase gene from Gordonia spp. Syst Appl Microbiol 33 53-59 [CrossRef] [PubMed] . ... 1 Department of Biological Sciences, Faculty of Science, Kuwait University, PO Box 5969, Safat 13060, Kuwait ...
Frontiers | Growth of Rhodococcus sp. strain BCP1 on gaseous n-alkanes: new metabolic insights and transcriptional analysis of...
... lyase were identified along with oxidoreductases involved in oxidation reactions downstream of the initial monooxygenase ... Here, both molecular and metabolic features of BCP1 cells grown on gaseous and short-chain n-alkanes (up to n-heptane) were ... Here, both molecular and metabolic features of BCP1 cells grown on gaseous and short-chain n-alkanes (up to n-heptane) were ... Two gene clusters, prmABCD and smoABCD-coding for Soluble Di-Iron Monooxgenases (SDIMOs) involved in gaseous n-alkanes ...
DeCS 2015 - Changed terms
CN09 02310 protein (Agrobacterium rhizogenes) - STRING interaction network
Alkane 1-monooxygenase; Derived by automated computational analysis using gene prediction method- Protein Homology (332 aa) ... Alkane 1-monooxygenase; Derived by automated computational analysis using gene prediction method- Protein Homology ... Alkane 1-monooxygenase; Derived by automated computational analysis using gene prediction method- Protein Homology ... Alkane 1-monooxygenase; Derived by automated computational analysis using gene prediction method- Protein Homology ...
Network Portal - Gene PA4038
4-hydroxyphenylacetate 3-monooxygenase small chain (NCBI). 192, 337. PA4187. PA4187. probable major facilitator superfamily ( ... alkane-1-monooxygenase (NCBI). 30, 192. PA3387. rhlG. beta-ketoacyl reductase (NCBI). 192, 550. ... POSITION A C G T 1 0.0 0.0 0.0 1.0 2 1.0 0.0 0.0 0.0 3 0.25 0.0 0.0 0.75 4 0.0 0.0 1.0 0.0 5 0.5 0.25 0.25 0.0 6 1.0 0.0 0.0 ... 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this ...
Network Portal - Gene PA2651
alkane-1-monooxygenase (NCBI). 30, 192. PA2584. pgsA. CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (NCBI) ... POSITION A C G T 1 1.0 0.0 0.0 0.0 2 1.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.0 4 1.0 0.0 0.0 0.0 5 0.0 0.0 1.0 0.0 6 0.0 0.0 0.0 1.0 7 ... POSITION A C G T 1 0.0 0.0 1.0 0.0 2 0.0 0.2 0.2 0.6 3 0.6 0.0 0.4 0.0 4 0.0 0.0 1.0 0.0 5 0.0 0.6 0.0 0.4 6 0.2 0.4 0.4 0.0 7 ... 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this ...
Browse EndoSite | RNAi screening of S2R+ cells in Drosophila Melanogaster
There is experimental evidence that it has the molecular function: alkane 1-monooxygenase activity. There is experimental ... 1; R2: Replicate No.2; R3: Replicate No. 3. Primary screen data - CG10248: SN. RN. WN. Fint1. Fint2. Fint3. Fint4. Fnum. Fmph1 ... 1. 65. 0.049072431. -0.121728901. -0.374768319. -0.386066868. 1.233482494. 1.752211326. 1.62871669. 1.814638184. 2.696060555. ...
WO2016161043 BIOCONVERSION OF SHORT-CHAIN HYDROCARBONS TO FUELS AND CHEMICALS
Alkane 1-monooxygenase (1.14.15.3). C12Y 203/01012 C. CHEMISTRY; METALLURGY. 12. BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; ... Oxidoreductases (1.). 0071. acting on paired donors with incorporation of molecular oxygen (1.14). 0077. with a reduced iron- ... Oxidoreductases (1.). 0071. acting on paired donors with incorporation of molecular oxygen (1.14). 0077. with a reduced iron- ... Oxidoreductases (1.). 0008. acting on the aldehyde or oxo group of donors (1.2). ...
DegradationEnzymeGeneHydroxylasesCytochrome p450Oxidation of alkanesMetabolismSubstratesHydroxylase systemBacterialBacteriaAliphaticPseudomonas putida GPo1AlcoholsFattyInitial oxidationHydrocarbonsMedium-chain alkanesHydrocarbonEthaneStrainEscherichiaTert -ButylDiiron monooxygenaseMicrobialMethane MonooxygenasesProteinCatalyticPathwayPathwaysOxidizeRibonucleotide reductaseRhodococcusRubA3ReductaseSelectivityMethanotrophsMetabolicAerobicMicrobiolMineralization
Degradation19
- Figure 1 - Schematic description of the alkane degradation pathway with the corresponding genes. (igem.org)
- To create the alkane degradation constructs a number of genes encoding for alkane degradation enzymes were synthesized and combined with promoters and ribosome binding sites obtained from the BioBrick distribution plates. (igem.org)
- This work describes the alkane degradation system of the n -alkane degrader actinobacterium Gordonia sp. (asm.org)
- A SoCg alkB disruption mutant that is completely unable to grow on n -triacontane was obtained, demonstrating the role of an AlkB-type AH system in degradation of solid n -alkanes. (asm.org)
- Five chromosomal genes ( alkM , rubA , rubB , alkR , and xcpR ) in at least three different loci are required for degradation of C 12 to C 18 alkanes in Acinetobacter sp. (asm.org)
- strain BCP1 was initially isolated for its ability to grow on gaseous n -alkanes, which act as inducers for the co-metabolic degradation of low-chlorinated compounds. (frontiersin.org)
- In this study we analyzed the functional diversity of genes for alkane hydroxylases, the enzymes responsible for converting alkanes to more labile alcohols, as found in the genomes of nineteen psychrophiles for which alkane degradation has not been reported. (biomedcentral.com)
- Thirteen genes in charge of the degradation of PAHs and alkanes had been determined, including 1 alkane 1-monooxygenase gene, 5 catechol 1,2-dioxygenase genes, 2 benzene 1,2-dioxygenase genes, and 5 naphthalene 1,2-dioxygenase genes. (chenglilab.org)
- Hexahydro-1,3,5-trinitro-1,3,5-triazine and its degradation products are toxic, mutagenic and carcinogenic to humans and other biological systems. (brenda-enzymes.org)
- Nevertheless, significant changes in the distribution patterns of short- and middle-chained alkanes suggest an interaction of MCPA and alkane degradation. (ufz.de)
- The alkane hydroxylase (AlkB), which catalyse the first reaction, is the key enzyme in the process of alkane degradation and has received increasing attention. (scielo.br)
- Degradation of alkanes is a widespread phenomenon in nature, and numerous microorganisms capable of utilizing these substrates as a carbon and energy source have been isolated and characterized (Wentzel, A., Ellingsen, T.E., Kotlar, H.-K., Zotchev, S.B. and Throne-Holst, M. 2007. (europa.eu)
- Extensive knowledge of the microbiology of long-chain n-alkanes degradation has been accumulated. (europa.eu)
- The primary route of degradation for alkanes is to the alcohol. (europa.eu)
- This chapter focuses on the aerobic degradation of hydrocarbons, with an emphasis on alkanes, and the applications of enzymes involved in these processes for biocatalysis. (asmscience.org)
- Pathways for the degradation of alkanes by terminal, subterminal, and biterminal oxidation. (asmscience.org)
- Strain DS-1 T is a non-pigmented, aerobic, heterotrophic bacterium and represents the first tier member of environmentally important bacterial communities that catalyze the complete degradation of synthetic laundry surfactants. (biomedcentral.com)
- The initial degradation as catalyzed by strain DS-1 T involves the activation and shortening of the alkyl-chain of the surfactant molecules, and the excretion of short-chain degradation intermediates. (biomedcentral.com)
- As bioremediation can be effective only where environmental conditions permit microbial growth and activity, its application often involves the manipulation of environmental parameters to allow microbial growth and degradation to proceed at a faster rate (Figure 1 ). (hindawi.com)
Enzyme12
- In enzymology, an alkane 1-monooxygenase (EC 1.14.15.3) is an enzyme that catalyzes the chemical reactions an alkane + reduced rubredoxin + O2 ⇌ {\displaystyle \rightleftharpoons } a primary alcohol + oxidized rubredoxin + H2O. (wikipedia.org)
- The systematic name of this enzyme class is alkane, reduced-rubredoxin:oxygen 1-oxidoreductase. (wikipedia.org)
- The direct ω-oxyfunctionalization of aliphatic alkanes in a regio- and chemoselective manner is efficiently catalyzed by monooxygenases such as the AlkBGT enzyme complex from Pseudomonas putida under mild conditions. (biomedcentral.com)
- Collectively, the results of this study suggest that GSPALK1 is the enzyme that catalyzes the initial oxidation of alkanes and ethers but is not involved in the later steps of alkane or ether metabolism. (usda.gov)
- Upon binding to the active site, 1-octyne is postulated to be oxidized to an oxirene that rapidly rearranges to a reactive ketene which covalently acylates nearby residues, resulting in enzyme inactivation. (biochemj.org)
- One or more recombinant genes encoding one or more enzymes having enzyme activities which catalyze the production of medium chain-length alkanes are identified and selected. (blogspot.com)
- The enzyme activities include: an alkane deformylative monooxygenase activity, a thioesterase activity, a carboxylic acid reductase activity, and a phosphopanthetheinyl transferase activity, a long-chain fatty acid CoA-ligase activity, and/or a long-chain acyl-CoA reductase activity. (blogspot.com)
- The oxygenases that are required for the initial activation of alkanes belong to several different enzyme classes, some of which act on medium- and long-chain alkanes, while others oxidize only short-chain alkanes. (asmscience.org)
- Studies of alkane hydroxylase (AH) gene diversity, coupled with information on substrate range, induction, enzyme kinetics, and host properties, should help in understanding and optimizing the biodegradative activity of indigenous hydrocarbon-degrading strains, benefit biocatalytic applications, and promote fundamental research on the activation of oxygen by enzymes and biomimetic catalysts. (asmscience.org)
- This structure shows changes in the substrate binding regions of the enzyme and a reduction in active site volume that are consistent with the observed changes in substrate specificity from fatty acids in the native enzyme to small alkanes in P450PMO. (unt.edu)
- The goal of the present work was to characterize microbial communities involved in short-chain alkane metabolism, namely methane, ethane and propane, in samples from a petroliferous (P) soil through clone libraries of the 16S rRNA gene of the Domains Bacteria and Archaea and the catabolic gene coding for the soluble di-iron monooxygenase (SDIMO) enzyme alpha subunit. (springeropen.com)
- Aerobic methanotrophs bacteria can oxidize methane through the enzyme methane monooxygenase (MMO). (biology-online.org)
Gene15
- AJ293344 Burkholderia cepacia partial alkB gene for putative alkane 1-monooxygenase. (atcc.org)
- L36602 Rhizobium meliloti (clone Rhizo70-1) heat shock protein 70 (hsp70) gene, complete cds. (atcc.org)
- L36603 Pseudomonas cepacia (clone Psudom70-1) heat shock protein 70 (hsp70) gene, complete cds. (atcc.org)
- The detection and phylogenetic analysis of the alkane 1-monooxygenase gene of members of the genus Rhodococcus , SYSTEMATIC AND APPLIED MICROBIOLOGY 38: pp. 1-7. (doktori.hu)
- Two gene clusters, prmABCD and smoABCD -coding for Soluble Di-Iron Monooxgenases (SDIMOs) involved in gaseous n -alkanes oxidation-were detected in the BCP1 genome. (frontiersin.org)
- A gene, CYP52L1, was identified, cloned and functionally characterized as an alkane-oxidizing cytochrome P450 (GSPALK1). (usda.gov)
- Post-transcriptional ds-RNA-mediated gene silencing of CYP52L1 severely reduced the ability of this fungus to oxidize alkanes and ethers, however, downstream metabolic steps in these pathways were unaffected. (usda.gov)
- The influence of the herbicide 2-methyl-4-chlorophenoxyacetic acid (MCPA) on the mineralization of litter-derived alkanes and the abundance of the alkane monooxygenase gene ( alkB ) in the detritusphere of Pisum sativum (L. (ufz.de)
- In the present study, the temporal and spatial variation of the abundance of the alkane monooxygenase gene alkB and 16S rRNA genes in different soil compartments was analysed in the presence or absence of 2-methyl-4-chlorophenoxyacetic acid (MCPA) after the addition of pea litter to soil in a microcosm study. (ufz.de)
- The alkane hydroxylase ( alkB ) gene of Pseudomonas putida GPo1 was constructed in a pCom8 expression vector, and the pCom8-GPo1 alkB plasmid was transformed into Escherichia coli DH5α. (scielo.br)
- In a previous work, it was reported that the Bacillus subtilis des gene codes for a unique desaturase, Des ( 1 ). (asm.org)
- It was previously shown that expression of the des gene in Escherichia coli resulted in desaturation of the palmitic moieties of the membrane phospholipids to give cis -5-hexadecenoic acid, indicating that the B . subtilis desaturase is a Δ5 acyl lipid desaturase ( 1 ). (asm.org)
- The bacterial communities and alkane monooxygenase gene fragment, alkB, were compared by denaturing gradient gel electrophoresis (DGGE). (ewha.ac.kr)
- Degenerate primers were used to amplify a portion of an alkane monooxygenase gene. (mysciencework.com)
- Soluble and particulate mono-oxygenases are known to oxidize the same compounds, and some of the gene diversity detected with primers that amplify membrane-bound methane mono-oxygenase (pMMO) and soluble MMO (sMMO) genes may well be due to short-chain alkane-degrading bacteria instead of methanotrophs. (asmscience.org)
Hydroxylases7
- Under aerobic conditions, activation is usually achieved by oxidation of one of the terminal methyl groups to generate the corresponding primary alcohol by alkane hydroxylases (AHs) ( 18 , 26 ). (asm.org)
- To identify possible mechanisms of low temperature optimization we compared putative alkane hydroxylases from these psychrophiles with homologues from nineteen taxonomically related mesophilic strains. (biomedcentral.com)
- These candidates were mostly related to the AlkB and cytochrome p450 alkane hydroxylases, but several homologues of the LadA and AlmA enzymes, significant for their ability to degrade long-chain alkanes, were also detected. (biomedcentral.com)
- These putative alkane hydroxylases showed significant differences in primary structure from their mesophile homologues, with preferences for specific amino acids and increased flexibility on loops, bends, and α-helices. (biomedcentral.com)
- In the most described cases, the n-alkane is oxidized to the corresponding primary alcohol by substrate-specific terminal monooxygenases/ hydroxylases. (europa.eu)
- This metabolism and excretion mechanism, suggesting low bioaccumulation potential, is expected to cover all of the components of HAB because all contain at least one linear alkyl chain with unblocked terminal carbons that are known substrates for terminal monooxygenases/hydroxylases as described by Wentzel et al. (europa.eu)
- The goals of this project are to investigate (1) the kinetics and stabilities of engineered cytochrome P450 (P450) small alkane hydroxylases and their evolutionary intermediates, (2) the structural basis for catalytic proficiency on small alkanes of these engineered P450s, and (3) the changes in redox control resulting from protein engineering. (unt.edu)
Cytochrome p4508
- Also known as Leukotriene-B(4) omega-hydroxylase 2 (CYPIVF3) (Cytochrome P450 4F3) (Cytochrome P450-LTB-omega) (Leukotriene-B(4) 20-monooxygenase 2). (mybiosource.com)
- RNAi silencing of a cytochrome P450 monooxygenase disrupts the ability of a filamentous fungus, Graphium sp. (usda.gov)
- Because the dirivatives of these compounds are commonly used as growth substrates by many organisms, the identification of this unique protein, which belongs to the cytochrome P450 monooxygenase family, provides knowledge about the biochemical step that allows Graphium to occupy this strange ecological niche. (usda.gov)
- Analysis of CYP52L1 suggests that it is a member of the CYP52 cytochrome P450 family, which is comprised of medium- and long-chain alkane-oxidizing enzymes found in yeasts. (usda.gov)
- 9. Directed Evolution of a Cytochrome P450 Monooxygenase for Alkane Oxidation. (njit.edu)
- The in vitro effects of alkanes, alcohols, and ketones on rat lung cytochrome P450-dependent alkoxyphenoxazone dealkylase activities. (cdc.gov)
- This inhibition was directed at the cytochrome-P450 dependent monooxygenase activity. (cdc.gov)
- Cytochrome P450 monooxygenases (P450s) are a diverse collection of enzymes acting on various endogenous and xenobiotic molecules. (tailieu.vn)
Oxidation of alkanes3
- In this work, we have generated a new non-heme iron(IV)-oxo complex, [Fe(IV)(O)(BQEN)]2+ (1) (BQEN = N,N'-dimethyl-N,N'-bis(8-quinolyl)ethane-1,2-diamine), that shows a high reactivity in the oxidation of alkanes and alcohols. (ewha.ac.kr)
- More importantly, we have obtained strong evidence that an iron(V)-oxo species is involved as an active oxidant in the catalytic oxidation of alkanes and alcohols by an iron catalyst and a terminal oxidant. (ewha.ac.kr)
- These ligands form diiron complexes, which rapidly and efficiently catalyzed the oxidation of alkanes with m-CPBA as an oxidant to show high A/K ratio, large turnover number, large turnover frequency in the catalytic oxidations. (nii.ac.jp)
Metabolism7
- We show that propane metabolism generated terminal and sub-terminal oxidation products such as 1- and 2-propanol, whereas 1-butanol was the only terminal oxidation product detected from n -butane metabolism. (frontiersin.org)
- Our present knowledge on the metabolism of short-chain n -alkanes is based on studies in Gram-negative bacteria, when the most common bacterial species isolated from natural habitats that utilize gaseous n -alkanes are Gram-positive strains belonging to the CMNR group (including Corynebacterium, Nocardia, Mycobacterium , and Rhodococcus genera). (frontiersin.org)
- Alkane monooxygenases initiate the metabolism of alkanes by catalyzing the oxidation of the alkanes to alcohols. (oregonstate.edu)
- This work will provide the detailed molecular description of a pathway of gaseous alkane metabolism (other than methane). (oregonstate.edu)
- However, metabolism data on linear alkyl chains includes conversion of the terminal carbons of linear alkyl chains (alkanes) to carboxylic acids followed by metabolism of the resulting fatty acids. (europa.eu)
- Bacterial metabolism of long-chain n-alkanes. (europa.eu)
- The major difference between the metabolism under aerobic conditions of alkanes and isoalkanes relates to the impact of the branching on the rate of metabolism (Fritsche and Hofrichter, 2000). (europa.eu)
Substrates5
- Alkanes of 6 to 22 carbons have been observed as substrates. (wikipedia.org)
- Bacteria can utilize n -alkanes as growth substrates under aerobic conditions through the use of a monooxygenase reaction. (frontiersin.org)
- Gaseous and liquid alkanes can serve as growth substrates for several bacteria. (oregonstate.edu)
- Subterminal oxidation has also been described both for long-chain n-alkane substrates up to C16 and for n-alkanes of shorter chain lengths (Wentzel et al. (europa.eu)
- 1, 2) "In general methylotrophs can use green-house gases such as carbon dioxide and methane as substrates to fulfill their energy and carbon needs. (kenyon.edu)
Hydroxylase system3
- Involvement of an Alkane Hydroxylase System of Gordonia sp. (asm.org)
- Among these, the alk system found in Pseudomonas putida GPo1, which degrades C 5 to C 12 n -alkanes, remains the most extensively characterized alkane hydroxylase system ( 27 ). (asm.org)
- Similar to the case for P. putida GPo1, the initial terminal alkane oxidation is also catalyzed by a three-component alkane hydroxylase system, which comprises an alkane monooxygenase (AlkM), rubredoxin (RubA), and rubredoxin reductase (RubB). (asm.org)
Bacterial1
- Phylogenetic diversity of bacterial and archaeal communities inhabiting the saline Lake Red located in Sovata, Romania , EXTREMOPHILES : LIFE UNDER EXTREME CONDITIONS 17: (1) pp. 87-98. (doktori.hu)
Bacteria7
- Enzymes involved in oxidation of long-chain n -alkanes are still not well known, especially those in Gram-positive bacteria. (asm.org)
- Alkanes constitute about 20 to 50% of crude oil, depending on the source of the oil, but living organisms, such as bacteria, plants, and some animals, also produce them as pheromones ( 4 ). (asm.org)
- Alkene monooxygenase (AMO) fromRhodococcus rhodochrous(formerly Nocardia corallina) B-276 belongs to a family of multicomponent nonheme binuclear iron-centre oxygenases that includes the soluble methane mono-oxygenases (sMMOs) found in some methane-oxidizing bacteria. (tailieu.vn)
- In contrast methanotrophic bacteria perform the desired transformation under ambient conditions, using methane monooxygenase (MMO) enzymes. (aimspress.com)
- Andria V, Reichenauer TG, Sessitsch A (2009) Expression of alkane monooxygenase (alkB) genes by plant-associated bacteria in the rhizosphere and endosphere of Italian ryegrass ( Lolium multiflorum L.) grown in diesel contaminated soil. (springer.com)
- The employability of various organisms like bacteria, fungi, algae, and plants for efficient bioremediation of pollutants has been reported [ 1 , 2 ]. (hindawi.com)
- Both bacteria and fungi rely on the participation of different intracellular and extracellular enzymes respectively for the remediation of recalcitrant and lignin and organopollutants [ 1 , 6 ]. (hindawi.com)
Aliphatic3
- Although many microorganisms are capable of degrading aliphatic hydrocarbons and they are readily isolated from contaminated and noncontaminated sites, relatively little is known about the molecular characteristics of their alkane-degradative systems. (asm.org)
- Volatile aliphatic n -alkanes comprise of gaseous and liquid n -alkanes up to n -heptane (C 7 ). (frontiersin.org)
- It also present almost everywhere that can cometabolize various aliphatic compounds like alkanes, and aromatic compounds in which methanotrophs have been extremely studied for the use in degrading chlorinated solvents to environmentally suitable application in soils, sediment, and groundwater. (biology-online.org)
Pseudomonas putida GPo13
- Pseudomonas putida GPo1 alkane hydroxylase (AlkB) is an integral membrane protein that catalyses the hydroxylation of medium-chain alkanes (C 3 -C 12 ). (biochemj.org)
- PCC6803 was engineered to synthesize the alkane monooxygenase AlkBGT from Pseudomonas putida GPo1. (readbyqxmd.com)
- This reaction, which is difficult to achieve by chemical means, is catalyzed by Escherichia coli featuring the monooxygenase system AlkBGT and the uptake facilitator AlkL from Pseudomonas putida GPo1. (readbyqxmd.com)
Alcohols2
- This system facilitates the initial oxidation step of C5-C13 alkanes along with that of C5-C8 cycloalkanes towards their respective alcohols. (igem.org)
- This protein facilitates the conversion of long-chain alkanes (from C15 up to at least C36) to their corresponding primary alcohols in the presence of flavin mononucleotide, oxygen and NADH. (igem.org)
Fatty2
- Catalyzes the omega- and (omega-1)-hydroxylation of various fatty acids such as laurate and palmitate. (uniprot.org)
- 1. Microsomal P450 and peroxisomal fatty acid oxidation activities were studied in liver of rats after long-term ethanol consumption. (semanticscholar.org)
Initial oxidation1
- After initial oxidation of the n-alkane, the corresponding alcohol is subsequently oxidized further by alcohol dehydrogenases and aldehyde dehydrogenase to the corresponding aldehyde and carboxylic acid, respectively. (europa.eu)
Hydrocarbons4
- Alkanes are saturated, linear hydrocarbons whose chain length can vary from 1 (in methane) to more than 50 carbon atoms. (asm.org)
- Gaseous n -alkanes ranging from ethane (C 2 ) to n -butane (C 4 ) are categorized as non-methane hydrocarbons. (frontiersin.org)
- Diesel oil is a complex mixture of different types of hydrocarbons (C 6 to C 22 ) and branched alkanes, such as 2,6,10,14-tetramethyl pentadecane (pristine) and 2,4,6,10-tetramethyl hexadecane (phytane). (scielo.br)
- Yeasts and fungi are often mentioned as alkane degraders, for example in connection with the production of single-cell proteins or organic acids and amino acids from hydrocarbons. (asmscience.org)
Medium-chain alkanes1
- U.S. Patent #9,034,629, issued on May 19, covers both the cyanobacterium and the process for directly converting CO2 into medium-chain alkanes, which are the molecular basis of diesel, jet fuel and gasoline. (blogspot.com)
Hydrocarbon4
- Our medium-chain hydrocarbon degrading strain contains the alkane hydroxylase (AH) system from ''Gordonia sp. (igem.org)
- Our medium-chain hydrocarbon degrading strain contains the alkane hydroxylase (AH) native to Gordonia sp. (igem.org)
- Primers based upon the sequences of the polycyclic aromatic hydrocarbon (PAH) dioxygenases nidAB (from Mycobacterium vanbaalenii strain PYR-1) and pdoA2B2 (from Mycobacterium sp. (mysciencework.com)
- Clones related to the ethene monooxygenase (EtnC) and methane monooxygenase (MmoX) coding genes occurred only in P soil, which also presented higher levels of methane and lower levels of ethane and propane, revealed by short-chain hydrocarbon measures. (springeropen.com)
Ethane3
- 본 연구에서는 새로운 non-heme iron(Ⅳ)-oxo 종인 [FeⅣ(O)(BQEN)]2+(1) (BQEN = N,N'-dimethyl-N,N'-bis(8-quinolyl)ethane-1,2-diamine)를 생성시켜 큰 반응성을 보여주었다. (ewha.ac.kr)
- Hydrogen-Atom Transfer Oxidation with H2O2 Catalyzed by [FeII(1,2-bis(2,2 '-bipyridyl-6-yl)ethane(H2O)(2)](2+): Likely Involvement of a (mu-Hydroxo)(mu-1,2-peroxo)diiron(III) Intermediate. (weizmann.ac.il)
- Using these, we were able to determine that CYP153A6 is proficient for hydroxylation of alkanes as small as ethane, an activity that has never been observed previously in any natural P450. (unt.edu)
Strain4
- The Alkanivore E.coli strain has been designed to carry the genes required for the conversion of medium (C 5 -C 13 ) and long chain (C 15 -C 36 ) alkanes. (igem.org)
- strain SoCg, which is able to grow on n -alkanes from dodecane (C 12 ) to hexatriacontane (C 36 ) as the sole C source. (asm.org)
- In addition, strain CH-2 could utilize phenanthrene, pyrene and a wide range of alkanes as a sole carbon and energy source. (mysciencework.com)
- Draft Genome Sequence of Nitrobacter vulgaris Strain Ab 1 , a Nitrite-Oxidizing Bacterium. (nih.gov)
Escherichia1
- The Gordonia SoCg alkB was heterologously expressed in Escherichia coli BL21 and in Streptomyces coelicolor M145, and both hosts acquired the ability to transform n -hexadecane into 1-hexadecanol, but the corresponding long-chain alcohol was never detected on n -triacontane. (asm.org)
Tert -Butyl1
- Search Medline for alkane 1-monooxygenase AND tert-Butyl alcohol . (ethz.ch)
Diiron monooxygenase2
Microbial2
- The transformation of environmental contaminants is a complex process that is influenced by the nature and amount of the contaminant present, the structure and dynamics of the indigenous microbial community, and the interplay of geochemical and biological factors at contaminated sites ( 1 , 14 , 26 , 36 ). (asm.org)
- In recent years, there have been intensive efforts to develop synthetic microbial platforms for the production, biosensing and bio-remediation of fossil fuel constituents such as alkanes. (readbyqxmd.com)
Methane Monooxygenases2
- soluble and particulate methane monooxygenases. (oregonstate.edu)
- Soluble methane monooxygenases (sMMO) are nonheme diiron enzymes that catalyze oxidation of various organic compounds. (nii.ac.jp)
Protein4
- The chromosome of N. farcinica , unlike that of Streptomyces ( 13 , 14 ), has a circular topology ( Fig. 1 ) and encodes 53 tRNA genes, three copies of ribosomal RNA operons, and 5,674 predicted protein-coding sequences. (pnas.org)
- Finally, proteomic studies revealed changes in the protein pattern induced by growth on gaseous- ( n -butane) and/or liquid ( n -hexane) short-chain n -alkanes as compared to growth on succinate. (frontiersin.org)
- Among the differently expressed protein spots, two chaperonins and an isocytrate lyase were identified along with oxidoreductases involved in oxidation reactions downstream of the initial monooxygenase reaction step. (frontiersin.org)
- In this paper, we identified the protein that converts propane, THF, MTBE, 1-4-dioxane into more palatable compounds that can be readily consumed by the fungus. (usda.gov)
Catalytic5
- This is the catalytic component of the AH system and oxidizes (cyclo)alkanes to their respective (cyclo)alkanols by transferring one oxygen atom from molecular oxygen to the alkane. (igem.org)
- Part I. Nonheme iron의 active site를 가지고 있는 산소화 효소들은 Biological system에서 dioxygen activation과 유기물질들과의 산화반응에 관여된다.1 이 효소들의 catalytic mechanism에서 metal-peroxo나 high-valent metal-oxo종은 bleomycin, superoxide reductase, α-ketoglutarate dioxygenase에 중요한 활성 중간체들로 밝혀졌다. (ewha.ac.kr)
- Iron(Ⅴ)-oxo 종은 nonheme iron catalyst에 의한 organic substrate의 산화반응에서 활성 산화제로서 생각되어지고 있다.1 최근에 Iron(Ⅴ)-oxo 중간체가 합성되고, 다양한 분광학적 기술로 characterization 되었지만,1 catalytic oxygenation 반응에서 활성산화제로서 Iron(Ⅴ)-oxo 종이 관여한다는 증거는 간접적으로 product analysis로부터 알 수 있다. (ewha.ac.kr)
- 그 예로는 Alkane hydroxylation 반응 생성물인 alcohol과 ketone의 selectivity, 4 이하의 H-KIE값, labeled water 실험에서 H218O의 18O가 생성물로의 incorporation가 있다.1 그렇지만, iron(Ⅳ)-oxo complex 역시 매우 큰 KIE 값을 갖고,2 H218O와의 산소교환을 보여준다.3 그러므로 catalytic oxygenation 반응에서 중간체들을(예를 들면 iron(Ⅳ)-oxo 또는 Iron(Ⅴ)-oxo )구분하기에는 어렵다. (ewha.ac.kr)
- 더욱 중요한 것은 Iron(Ⅴ)-oxo 종이 Fe catalyst와 terminal oxidant에 의한 alkane과 alcohol의 catalytic oxidation 반응에서 활성산화제라는 강력한 증거를 알아낼 수 있었다. (ewha.ac.kr)
Pathway2
- In this study, we investigated the genetic underpinnings that enable this fungus to catalyze the first step in the alkane and ether oxidation pathway. (usda.gov)
- reported the cloning and characterization of several genes from M. austroafricanum IFP 2012 involved in the lower portion of the MTBE pathway (from 2-methyl-2-hydroxy-1-propanol to 2-hydroxyisobutyrate). (ethz.ch)
Pathways1
- We identify an array of alkane monooxygenases, osmoprotectants, siderophores and micronutrient-scavenging pathways. (rcsb.org)
Oxidize2
- The Baeyer-Villiger monooxygenase (BVMO), 4-hydroxy-acetophenone monooxygenase (HAPMO), uses NADPH andO2 to oxidize a variety of aromatic ketones and sulfides. (tailieu.vn)
- Transcriptomic data delineate active members of the community and give insights that Acinetobacter species completely oxidize alkanes into carbon dioxide with the involvement of oxygen, and Archaeoglobus species mainly ferment alkanes to generate acetate which could be consumed by Methanosaeta species. (biomedcentral.com)
Ribonucleotide reductase1
- The first metrical data has been obtained for the high valent intermediates Q and X of methane monooxygenase and ribonucleotide reductase, respectively. (wikigenes.org)
Rhodococcus1
- Sequence analysis of 16S rRNA, gyrB and catA genes and DNA-DNA hybridization reveal that Rhodococcus jialingiae is a later synonym of Rhodococcus qingshengii , INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 64: (Part 1) pp. 298-301. (doktori.hu)
RubA31
- Quantitative reverse transcription-PCR showed that the genes encoding AlkB (alkane 1-monooxygenase), RubA3 (rubredoxin), RubA4 (rubredoxin), and RubB (rubredoxin reductase) were induced by both n -hexadecane and n -triacontane, which were chosen as representative long-chain liquid and solid n -alkane molecules, respectively. (asm.org)
Reductase2
- The initial terminal oxidation of the alkane substrate to a 1-alkanol is catalyzed by a three-component alkane hydroxylase complex consisting of a particulate nonheme integral membrane alkane monooxygenase (AlkB) and two soluble proteins, rubredoxin (AlkG) and rubredoxin reductase (AlkT). (asm.org)
- P450 BM3 (CYP102A1) from Bacillus megaterium is a self-sufficient monooxygenase as it is fused to its redox partner, an eukaryotic-like diflavin reductase. (springeropen.com)
Selectivity1
- His research interests include biochemistry and bioengineering of methane monooxygenase, mechanism of molecular recognition and product selectivity of alkane monooxygenase, and oxidative stress due to the oxidation reaction with tyrosinase. (springer.com)
Methanotrophs1
- Some degrees of methanotrophs have a genetic ability to produce a soluble methane monooxygenase that can catalyze the fast corrosion of environmental contaminants including trichloroethylene. (biology-online.org)
Metabolic3
- Here, both molecular and metabolic features of BCP1 cells grown on gaseous and short-chain n -alkanes (up to n -heptane) were examined in detail. (frontiersin.org)
- Alkanes are chemically quite inert and have to be activated to allow further metabolic steps to take place. (scielo.br)
- 3) Using small-subunit 16S rRNAs (1) and comparing metabolic/ phylogenetic similarities and differences (2) between M. flagellatus and its relatives, scientists have determined that Methylobacillus flagellatus (betaproteobacteria) is more closely related to Methylobacterium extorquens (alphaproteobacteria) and Methylococcus capsulatus (gammaproteobacteria), than to Methylibium petroleiphilum (betaproteobacteria). (kenyon.edu)
Aerobic1
- Formation of Alkanes by Aerobic Carbon-Carbon Bond Coupling Reactions Catalyzed by a Phosphovanadomolybdic Acid. (weizmann.ac.il)
Microbiol1
- Environ Microbiol Rep 1: 285-292. (aimspress.com)
Mineralization1
- Mineralization assays and reverse transcriptase-polymerase chain reaction (RT-PCR) analysis indicated that the alkane monooxygenase was constitutively expressed, while nidAB and pdoA2B2 were expressed only in the presence of PAHs. (mysciencework.com)