Alkanes: The generic name for the group of aliphatic hydrocarbons Cn-H2n+2. They are denoted by the suffix -ane. (Grant & Hackh's Chemical Dictionary, 5th ed)Alkane 1-Monooxygenase: A P450 oxidoreductase that catalyzes the hydroxylation of the terminal carbon of linear hydrocarbons such as octane and FATTY ACIDS in the omega position. The enzyme may also play a role in the oxidation of a variety of structurally unrelated compounds such as XENOBIOTICS, and STEROIDS.Oxygenases: Oxidases that specifically introduce DIOXYGEN-derived oxygen atoms into a variety of organic molecules.Mixed Function Oxygenases: Widely distributed enzymes that carry out oxidation-reduction reactions in which one atom of the oxygen molecule is incorporated into the organic substrate; the other oxygen atom is reduced and combined with hydrogen ions to form water. They are also known as monooxygenases or hydroxylases. These reactions require two substrates as reductants for each of the two oxygen atoms. There are different classes of monooxygenases depending on the type of hydrogen-providing cosubstrate (COENZYMES) required in the mixed-function oxidation.Kynurenine 3-Monooxygenase: An NADPH-dependent flavin monooxygenase that plays a key role in the catabolism of TRYPTOPHAN by catalyzing the HYDROXYLATION of KYNURENINE to 3-hydroxykynurenine. It was formerly characterized as EC 1.14.1.2 and EC 1.99.1.5.ButanesMethylococcaceae: A family of gram-negative, aerobic bacteria utilizing only one-carbon organic compounds and isolated from in soil and water.Methylococcus capsulatus: A species of METHYLOCOCCUS which forms capsules and is capable of autotrophic carbon dioxide fixation. (From Bergey's Manual of Determinative Bacteriology, 9th ed)Cytochrome P-450 Enzyme System: A superfamily of hundreds of closely related HEMEPROTEINS found throughout the phylogenetic spectrum, from animals, plants, fungi, to bacteria. They include numerous complex monooxygenases (MIXED FUNCTION OXYGENASES). In animals, these P-450 enzymes serve two major functions: (1) biosynthesis of steroids, fatty acids, and bile acids; (2) metabolism of endogenous and a wide variety of exogenous substrates, such as toxins and drugs (BIOTRANSFORMATION). They are classified, according to their sequence similarities rather than functions, into CYP gene families (>40% homology) and subfamilies (>59% homology). For example, enzymes from the CYP1, CYP2, and CYP3 gene families are responsible for most drug metabolism.Biodegradation, Environmental: Elimination of ENVIRONMENTAL POLLUTANTS; PESTICIDES and other waste using living organisms, usually involving intervention of environmental or sanitation engineers.Pseudomonas: A genus of gram-negative, aerobic, rod-shaped bacteria widely distributed in nature. Some species are pathogenic for humans, animals, and plants.Petroleum: Naturally occurring complex liquid hydrocarbons which, after distillation, yield combustible fuels, petrochemicals, and lubricants.Oxidation-Reduction: A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).Rhodococcus: A bacterial genus of the order ACTINOMYCETALES.Methylosinus trichosporium: A species of METHYLOSINUS which is capable of degrading trichloroethylene and other organic pollutants.HydrocarbonsOctanes: Eight-carbon saturated hydrocarbon group of the methane series. Include isomers and derivatives.Camphor 5-Monooxygenase: A soluble cytochrome P-450 enzyme that catalyzes camphor monooxygenation in the presence of putidaredoxin, putidaredoxin reductase, and molecular oxygen. This enzyme, encoded by the CAMC gene also known as CYP101, has been crystallized from bacteria and the structure is well defined. Under anaerobic conditions, this enzyme reduces the polyhalogenated compounds bound at the camphor-binding site.Rubredoxins: A class of iron-sulfur proteins that contains one iron coordinated to the sulfur atom of four cysteine residues. (McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)Alkenes: Unsaturated hydrocarbons of the type Cn-H2n, indicated by the suffix -ene. (Grant & Hackh's Chemical Dictionary, 5th ed, p408)Methane: The simplest saturated hydrocarbon. It is a colorless, flammable gas, slightly soluble in water. It is one of the chief constituents of natural gas and is formed in the decomposition of organic matter. (Grant & Hackh's Chemical Dictionary, 5th ed)Hydroxylation: Placing of a hydroxyl group on a compound in a position where one did not exist before. (Stedman, 26th ed)PropaneNitrosomonas: A genus of gram-negative, ellipsoidal or rod-shaped bacteria whose major source of energy and reducing power is from the oxidation of ammonia to nitrite. Its species occur in soils, oceans, lakes, rivers, and sewage disposal systems.Flavin-Adenine Dinucleotide: A condensation product of riboflavin and adenosine diphosphate. The coenzyme of various aerobic dehydrogenases, e.g., D-amino acid oxidase and L-amino acid oxidase. (Lehninger, Principles of Biochemistry, 1982, p972)Pseudomonas putida: A species of gram-negative, aerobic bacteria isolated from soil and water as well as clinical specimens. Occasionally it is an opportunistic pathogen.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Gordonia Bacterium: A genus of gram-positive BACTERIA in the family Gordoniaceae, isolated from soil and from sputa of patients with chest disorders. It is also used for biotransformation of natural products.Benzydamine: A benzyl-indazole having analgesic, antipyretic, and anti-inflammatory effects. It is used to reduce post-surgical and post-traumatic pain and edema and to promote healing. It is also used topically in treatment of RHEUMATIC DISEASES and INFLAMMATION of the mouth and throat.Tyrosine 3-Monooxygenase: An enzyme that catalyzes the conversion of L-tyrosine, tetrahydrobiopterin, and oxygen to 3,4-dihydroxy-L-phenylalanine, dihydrobiopterin, and water. EC 1.14.16.2.Squalene Monooxygenase: The second enzyme in the committed pathway for CHOLESTEROL biosynthesis, this enzyme catalyzes the first oxygenation step in the biosynthesis of STEROLS and is thought to be a rate limiting enzyme in this pathway. Specifically, this enzyme catalyzes the conversion of SQUALENE to (S)-squalene-2,3-epoxide.Oxidoreductases: The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)Substrate Specificity: A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.FMN Reductase: An enzyme that utilizes NADH or NADPH to reduce FLAVINS. It is involved in a number of biological processes that require reduced flavin for their functions such as bacterial bioluminescence. Formerly listed as EC 1.6.8.1 and EC 1.5.1.29.NADPH-Ferrihemoprotein Reductase: A flavoprotein that catalyzes the reduction of heme-thiolate-dependent monooxygenases and is part of the microsomal hydroxylating system. EC 1.6.2.4.Amidine-Lyases: These enzymes catalyze the elimination of ammonia from amidines with the formation of a double bond. EC 4.3.2.Multienzyme Complexes: Systems of enzymes which function sequentially by catalyzing consecutive reactions linked by common metabolic intermediates. They may involve simply a transfer of water molecules or hydrogen atoms and may be associated with large supramolecular structures such as MITOCHONDRIA or RIBOSOMES.Flavins: Derivatives of the dimethylisoalloxazine (7,8-dimethylbenzo[g]pteridine-2,4(3H,10H)-dione) skeleton. Flavin derivatives serve an electron transfer function as ENZYME COFACTORS in FLAVOPROTEINS.Gram-Negative Aerobic Bacteria: A large group of aerobic bacteria which show up as pink (negative) when treated by the gram-staining method. This is because the cell walls of gram-negative bacteria are low in peptidoglycan and thus have low affinity for violet stain and high affinity for the pink dye safranine.Alcanivoraceae: A family of halophilic bacteria in the order Oceanospirillales. Its principal carbon and energy sources are linear-chain ALKANES and their derivatives.Phenol: An antiseptic and disinfectant aromatic alcohol.EthaneTrichloroethylene: A highly volatile inhalation anesthetic used mainly in short surgical procedures where light anesthesia with good analgesia is required. It is also used as an industrial solvent. Prolonged exposure to high concentrations of the vapor can lead to cardiotoxicity and neurological impairment.Toluene: A widely used industrial solvent.Microsomes, Liver: Closed vesicles of fragmented endoplasmic reticulum created when liver cells or tissue are disrupted by homogenization. They may be smooth or rough.Kinetics: The rate dynamics in chemical or physical systems.Methylocystaceae: A family of gram-negative methanotrophs in the order Rhizobiales, distantly related to the nitrogen-fixing and phototrophic bacteria.Catalysis: The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Hydrocarbons, Chlorinated: Hydrocarbon compounds with one or more of the hydrogens replaced by CHLORINE.Waxes: A plastic substance deposited by insects or obtained from plants. Waxes are esters of various fatty acids with higher, usually monohydric alcohols. The wax of pharmacy is principally yellow wax (beeswax), the material of which honeycomb is made. It consists chiefly of cerotic acid and myricin and is used in making ointments, cerates, etc. (Dorland, 27th ed)Beijerinckiaceae: A family of aerobic gram-negative rods that are nitrogen fixers. They are highly viscous, and appear as a semitransparent slime in giant colonies.Alcohols: Alkyl compounds containing a hydroxyl group. They are classified according to relation of the carbon atom: primary alcohols, R-CH2OH; secondary alcohols, R2-CHOH; tertiary alcohols, R3-COH. (From Grant & Hackh's Chemical Dictionary, 5th ed)Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.NADP: Nicotinamide adenine dinucleotide phosphate. A coenzyme composed of ribosylnicotinamide 5'-phosphate (NMN) coupled by pyrophosphate linkage to the 5'-phosphate adenosine 2',5'-bisphosphate. It serves as an electron carrier in a number of reactions, being alternately oxidized (NADP+) and reduced (NADPH). (Dorland, 27th ed)Oxidoreductases, O-Demethylating: Drug metabolizing enzymes which oxidize methyl ethers. Usually found in liver microsomes.7-Alkoxycoumarin O-Dealkylase: A drug-metabolizing enzyme found in the hepatic, placental and intestinal microsomes that metabolizes 7-alkoxycoumarin to 7-hydroxycoumarin. The enzyme is cytochrome P-450- dependent.Pseudomonas mendocina: A species of gram-negative bacteria in the genus PSEUDOMONAS, which is found in SOIL and WATER.Chlorophenols: Phenols substituted with one or more chlorine atoms in any position.Acinetobacter: A genus of gram-negative bacteria of the family MORAXELLACEAE, found in soil and water and of uncertain pathogenicity.Biotransformation: The chemical alteration of an exogenous substance by or in a biological system. The alteration may inactivate the compound or it may result in the production of an active metabolite of an inactive parent compound. The alterations may be divided into METABOLIC DETOXICATION, PHASE I and METABOLIC DETOXICATION, PHASE II.Genes, Bacterial: The functional hereditary units of BACTERIA.Bacterial Proteins: Proteins found in any species of bacterium.Acetic Anhydrides: Compounds used extensively as acetylation, oxidation and dehydrating agents and in the modification of proteins and enzymes.Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.Dichloroethylenes: Toxic chlorinated unsaturated hydrocarbons. Include both the 1,1- and 1,2-dichloro isomers. Both isomers are toxic, but 1,1-dichloroethylene is the more potent CNS depressant and hepatotoxin. It is used in the manufacture of thermoplastic polymers.Oxygen: An element with atomic symbol O, atomic number 8, and atomic weight [15.99903; 15.99977]. It is the most abundant element on earth and essential for respiration.Hydrocarbons, Aromatic: Organic compounds containing carbon and hydrogen in the form of an unsaturated, usually hexagonal ring structure. The compounds can be single ring, or double, triple, or multiple fused rings.Hydrocarbons, HalogenatedAcetyleneEscherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Phylogeny: The relationships of groups of organisms as reflected by their genetic makeup.Multigene Family: A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)Pseudomonas stutzeri: A species of gram-negative bacteria in the genus PSEUDOMONAS, containing multiple genomovars. It is distinguishable from other pseudomonad species by its ability to use MALTOSE and STARCH as sole carbon and energy sources. It can degrade ENVIRONMENTAL POLLUTANTS and has been used as a model organism to study denitrification.KetonesAmmonia: A colorless alkaline gas. It is formed in the body during decomposition of organic materials during a large number of metabolically important reactions. Note that the aqueous form of ammonia is referred to as AMMONIUM HYDROXIDE.Microsomes: Artifactual vesicles formed from the endoplasmic reticulum when cells are disrupted. They are isolated by differential centrifugation and are composed of three structural features: rough vesicles, smooth vesicles, and ribosomes. Numerous enzyme activities are associated with the microsomal fraction. (Glick, Glossary of Biochemistry and Molecular Biology, 1990; from Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)Pseudomonas fluorescens: A species of nonpathogenic fluorescent bacteria found in feces, sewage, soil, and water, and which liquefy gelatin.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Acetone: A colorless liquid used as a solvent and an antiseptic. It is one of the ketone bodies produced during ketoacidosis.Copper: A heavy metal trace element with the atomic symbol Cu, atomic number 29, and atomic weight 63.55.Enzyme Induction: An increase in the rate of synthesis of an enzyme due to the presence of an inducer which acts to derepress the gene responsible for enzyme synthesis.Chloroquinolinols: 8-Hydroxyquinolinols chlorinated on the number 5 and/or 7 carbon atom(s). They are antibacterial, antiprotozoal, and antidiarrheal, especially in amebiasis, and have also been used as antiseborrheics. The compounds are mostly used topically, but have been used also as animal feed additives. They may cause optic and other neuropathies and are most frequently administered in combination with other agents.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Chromatography, Gas: Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix.Tryptophan Hydroxylase: An enzyme that catalyzes the hydroxylation of TRYPTOPHAN to 5-HYDROXYTRYPTOPHAN in the presence of NADPH and molecular oxygen. It is important in the biosynthesis of SEROTONIN.Agrocybe: A genus of saprobic mushrooms in the family Bolbitiaceae that grow in grass, dung, garden mulch, or in woods.Epoxy Compounds: Organic compounds that include a cyclic ether with three ring atoms in their structure. They are commonly used as precursors for POLYMERS such as EPOXY RESINS.Molecular Structure: The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.Iron: A metallic element with atomic symbol Fe, atomic number 26, and atomic weight 55.85. It is an essential constituent of HEMOGLOBINS; CYTOCHROMES; and IRON-BINDING PROTEINS. It plays a role in cellular redox reactions and in the transport of OXYGEN.Glyceryl Ethers: Compounds in which one or more of the three hydroxyl groups of glycerol are in ethereal linkage with a saturated or unsaturated aliphatic alcohol; one or two of the hydroxyl groups of glycerol may be esterified. These compounds have been found in various animal tissue.Flavin Mononucleotide: A coenzyme for a number of oxidative enzymes including NADH DEHYDROGENASE. It is the principal form in which RIBOFLAVIN is found in cells and tissues.4-Hydroxybenzoate-3-Monooxygenase: A flavoprotein that catalyzes the synthesis of protocatechuic acid from 4-hydroxybenzoate in the presence of molecular oxygen. EC 1.14.13.2.Models, Chemical: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.Rhodium: Rhodium. A hard and rare metal of the platinum group, atomic number 45, atomic weight 102.905, symbol Rh. (Dorland, 28th ed)Benzoate 4-Monooxygenase: An enzyme that catalyzes the oxidation of BENZOATE to 4-hydroxybenzoate. It requires IRON and tetrahydropteridine.

The PalkBFGHJKL promoter is under carbon catabolite repression control in Pseudomonas oleovorans but not in Escherichia coli alk+ recombinants. (1/260)

The alk genes are located on the OCT plasmid of Pseudomonas oleovorans and encode an inducible pathway for the utilization of n-alkanes as carbon and energy sources. We have investigated the influence of alternative carbon sources on the induction of this pathway in P. oleovorans and Escherichia coli alk+ recombinants. In doing so, we confirmed earlier reports that induction of alkane hydroxylase activity in pseudomonads is subject to carbon catabolite repression. Specifically, synthesis of the monooxygenase component AlkB is repressed at the transcriptional level. The alk genes have been cloned into plasmid pGEc47, which has a copy number of about 5 to 10 per cell in both E. coli and pseudomonads. Pseudomonas putida GPo12 is a P. oleovorans derivative cured of the OCT plasmid. Upon introduction of pGEc47 in this strain, carbon catabolite repression of alkane hydroxylase activity was reduced significantly. In cultures of recombinant E. coli HB101 and W3110 carrying pGEc47, induction of AlkB and transcription of the alkB gene were no longer subject to carbon catabolite repression. This suggests that carbon catabolite repression of alkane degradation is regulated differently in Pseudomonas and in E. coli strains. These results also indicate that PalkBFGHJKL, the Palk promoter, might be useful in attaining high expression levels of heterologous genes in E. coli grown on inexpensive carbon sources which normally trigger carbon catabolite repression of native expression systems in this host.  (+info)

Characterization of cytochrome P450 expression in human oesophageal mucosa. (2/260)

The expression of cytochrome (CYP) P450 enzymes in human oesophageal mucosa was investigated in a total of 25 histologically non-neoplastic surgical tissue specimens by using specific antibodies in immunoblots and by RT-PCR mRNA analysis. The presence of CYP1A, 2E1, 3A and 4A enzymes was demonstrated by both techniques; CYP2A reactive protein was also detected by immunoblot. The presence of CYP4B1 mRNA was established but no specific antibody was available for detection of the corresponding protein by immunoblot. CYP2B6/7 mRNA was not detected in any sample. The mRNA transcripts for CYP1A1, 2E1, 4A11 and 4B1 were consistently detected in the majority of samples (>84%), whereas CYP1A2 mRNA was only detected in 11 of 19 specimens examined. An RT-PCR method to differentiate CYP3A4 and 3A5 mRNA was developed. This demonstrated CYP3A5 mRNA expression in all samples tested, whereas CYP3A4 mRNA was not detectable, suggesting that CYP3A5 is the major CYP3A protein in human oesophagus. There were significant interindividual variations in the amount of proteins, ranging from 8-fold for CYP4A to 43-fold for CYP2E1. For each patient, data on exposure to risk factors for oesophageal cancer were available, including tobacco smoke, alcohol, gastro-oesophageal reflux and hot beverage consumption. None of these risk factors or other patient characteristics (age, sex, tumour location and tumour stage) were correlated with the protein level of the individual CYP enzymes as determined by quantitation of immunoblot staining. However, the small series of samples precludes any strong conclusion concerning the lack of such correlations. There were no differences between squamous cell carcinomas and adenocarcinomas in either the qualitative or quantitative expression of the CYP enzymes. These data demonstrate that a range of CYP enzymes are expressed in human oesophageal mucosa and indicate that this tissue has the capacity to activate chemical carcinogens to reactive DNA binding metabolites.  (+info)

Role of the alternative sigma factor sigmaS in expression of the AlkS regulator of the Pseudomonas oleovorans alkane degradation pathway. (3/260)

The AlkS protein activates transcription from the PalkB promoter, allowing the expression of a number of genes required for the assimilation of alkanes in Pseudomonas oleovorans. We have identified the promoter from which the alkS gene is transcribed, PalkS, and analyzed its expression under different conditions and genetic backgrounds. Transcription from PalkS was very low during the exponential phase of growth and increased considerably when cells reached the stationary phase. The PalkS -10 region was similar to the consensus described for promoters recognized by Escherichia coli RNA polymerase bound to the alternative sigma factor sigmaS, which directs the expression of many stationary-phase genes. Reporter strains containing PalkS-lacZ transcriptional fusions showed that PalkS promoter is very weakly expressed in a Pseudomonas putida strain bearing an inactivated allele of the gene coding for sigmaS, rpoS. When PalkS was transferred to E. coli, transcription started at the same site and expression was higher in stationary phase only if sigmaS-RNA polymerase was present. The low levels of AlkS protein generated in the absence of sigmaS were enough to support a partial induction of the PalkB promoter. The -10 and -35 regions of PalkS promoter also show some similarity to the consensus recognized by sigmaD-RNA polymerase, the primary form of RNA polymerase. We propose that in exponential phase PalkS is probably recognized both by sigmaD-RNA polymerase (inefficiently) and by sigmaS-RNA polymerase (present at low levels), leading to low-level expression of the alkS gene. sigmaS-RNA polymerase would be responsible for the high level of activity of PalkS observed in stationary phase.  (+info)

Insulin differentially affects xenobiotic-enhanced, cytochrome P-450 (CYP)2E1, CYP2B, CYP3A, and CYP4A expression in primary cultured rat hepatocytes. (4/260)

Uncontrolled diabetes results in enhanced expression of cytochrome P-450 (CYP)2E1, CYP2B, CYP3A, and CYP4A. Because of the simultaneous and confounding metabolic and hormonal changes that occur in vivo as a consequence of diabetes, primary cultured rat hepatocytes provide an excellent model system for examination of the effects of insulin on P-450 expression and on xenobiotic-mediated P-450 expression. In the present study, we examined the effects of insulin on pyridine-, phenobarbital-, and ciprofibrate-mediated expression of CYP2E1, CYP2B, CYP3A, and CYP4A in primary cultured rat hepatocytes. Pyridine addition to primary rat hepatocytes cultured in the presence of 1 nM insulin or in the absence of insulin resulted in a 3.5-fold and 3-fold enhancement in CYP2E1 protein expression, respectively, in the absence of any pyridine-mediated increase in mRNA expression. In contrast, hepatocytes cultured in the standard concentration of 1 microM insulin resulted in only a 2-fold increase in protein expression. Thus, the fold-induction of CYP2E1 protein in response to pyridine was 1.5- to 1.8-fold greater in either the absence of insulin or in the presence of 1 nM insulin, respectively, than that monitored in the presence of 1 microM insulin. To examine whether insulin effects on xenobiotic-mediated CYP2E1 expression were selective, insulin effects on xenobiotic-mediated expression of transcriptionally regulated CYP2B, CYP3A, and CYP4A were examined. Pyridine- or phenobarbital-mediated induction of CYP2B mRNA and protein expression in hepatocytes was suppressed by as much as 80% at lower insulin levels (0 and 1 nM), relative to the level monitored in the presence of 1 microM insulin. Omitting insulin from the medium resulted in a 50% decrease in CYP3A mRNA levels in response to phenobarbital treatment and a 30% decrease in CYP4A mRNA levels in response to ciprofibrate treatment, relative to the level obtained in response to these treatments in the presence of 1 microM insulin. The results of this study demonstrate that decreasing the insulin level in the primary hepatocyte culture medium enhanced xenobiotic-mediated CYP2E1 expression, whereas lower insulin levels suppressed xenobiotic-mediated CYP2B, CYP3A, and CYP4A expression in this cell culture system.  (+info)

Hypoxia-induced production of 12-hydroxyeicosanoids in the corneal epithelium: involvement of a cytochrome P-4504B1 isoform. (5/260)

The corneal epithelium metabolizes arachidonic acid by a cytochrome P-450 (CYP)-mediated activity to 12-hydroxy-5,8,11, 14-eicosatetraenoic acid (12(R)-HETE) and 12-hydroxy-5,8, 14-eicosatrienoic acid (12(R)-HETrE ). Both metabolites possess potent inflammatory properties, with 12(R)-HETrE being a powerful angiogenic factor, and they assume the role of inflammatory mediators in hypoxia- and chemical-induced injury in the cornea in vivo and in vitro. We used a model of corneal organ culture that exhibits hypoxia-induced epithelial CYP-dependent 12(R)-HETE and 12(R)-HETrE synthesis for isolating, identifying, and characterizing the CYP protein responsible for these eicosanoid syntheses. Northern analysis revealed the presence of a CYP4A-hybridizable mRNA, the levels of which were increased after hypoxia. Reverse transcription-polymerase chain reaction analysis with primers specific for the CYP4A family led to the isolation of a 671-base pair fragment with a 98.8% sequence homology to the rabbit lung CYP4B1 isoform, of which the levels in the corneal epithelium were greatly increased under hypoxic conditions. Moreover, phenobarbital, an inducer of hepatic CYP4B1 in the rabbit, also induced 12-HETE and 12-HETrE synthesis. Antibodies against CYP4B1, but not against CYP4A1, inhibited hypoxia-, clofibrate-, and phenobarbital-induced 12-HETE and 12-HETrE synthesis. These results suggest the involvement of a CYP4B1 isoform in the corneal epithelial synthesis of these eicosanoids in response to hypoxia.  (+info)

Arachidonic acid and PGE2 regulation of hepatic lipogenic gene expression. (6/260)

N-6 polyunsaturated fatty acids (PUFA) suppress hepatic and adipocyte de novo lipogenesis by inhibiting the transcription of genes encoding key lipogenic proteins. In cultured 3T3-L1 adipocytes, arachidonic acid (20:4,n-6) suppression of lipogenic gene expression requires cyclooxygenase (COX) activity. In this study, we found no evidence to support a role for COX-1 or -2 in the 20:4,n-6 inhibition of hepatocyte lipogenic gene expression. In contrast to L1 preadipocytes, adipocytes and rat liver, RT-PCR and Western analyses did not detect COX-1 or COX-2 expression in cultured primary hepatocytes. Moreover, the COX inhibitor, flurbiprofen, did not affect the 20:4,n-6 regulation of lipogenic gene expression in primary hepatocytes. Despite the absence of COX-1 and -2 expression in primary hepatocytes, prostaglandins (PGE2 and PGF2alpha) suppressed fatty acid synthase, l-pyruvate kinase, and the S14 protein mRNA, while having no effect on acyl-CoA oxidase or CYP4A2 mRNA. Using PGE2 receptor agonist, the PGE2 effect on lipogenic gene expression was linked to EP3 receptors. PGE2 inhibited S14CAT activity in transfected primary hepatocytes and targeted the S14 PUFA-response region located -220 to -80 bp upstream from the transcription start site. Taken together, these studies show that COX-1 and COX-2 do not contribute to the n-6 PUFA suppression of hepatocyte lipogenic gene expression. However, cyclooxygenase products from non-parenchymal cells can act on parenchymal cells through a paracrine process and mimic the effects of n-6 PUFA on lipogenic gene expression.  (+info)

Kinetic profile of the rat CYP4A isoforms: arachidonic acid metabolism and isoform-specific inhibitors. (7/260)

20-Hydroxyeicosatetraenoic acid (HETE), the cytochrome P-450 (CYP) 4A omega-hydroxylation product of arachidonic acid, has potent biological effects on renal tubular and vascular functions and on the control of arterial pressure. We have expressed high levels of the rat CYP4A1, -4A2, -4A3, and -4A8 cDNAs, using baculovirus and Sf 9 insect cells. Arachidonic acid omega- and omega-1-hydroxylations were catalyzed by three of the CYP4A isoforms; the highest catalytic efficiency of 947 nM-1. min-1 for CYP4A1 was followed by 72 and 22 nM-1. min-1 for CYP4A2 and CYP4A3, respectively. CYP4A2 and CYP4A3 exhibited an additional arachidonate 11,12-epoxidation activity, whereas CYP4A1 operated solely as an omega-hydroxylase. CYP4A8 did not catalyze arachidonic or linoleic acid but did have a detectable lauric acid omega-hydroxylation activity. The inhibitory activity of various acetylenic and olefinic fatty acid analogs revealed differences and indicated isoform-specific inhibition. These studies suggest that CYP4A1, despite its low expression in extrahepatic tissues, may constitute the major source of 20-HETE synthesis. Moreover, the ability of CYP4A2 and -4A3 to catalyze the formation of two opposing biologically active metabolites, 20-HETE and 11, 12-epoxyeicosatrienoic acid, may be of great significance to the regulation of vascular tone.  (+info)

Regulation of P-450 4A activity in the glomerulus of the rat. (8/260)

We recently reported that an enzyme of the cytochrome P-450 4A family is expressed in the glomerulus, but there is no evidence that 20-hydroxyeicosatetraenoic acid (20-HETE) can be produced by this tissue. The purpose of present study was to determine whether glomeruli isolated from the kidney of rats can produce 20-HETE and whether the production of this metabolite is regulated by nitric oxide (NO) and dietary salt intake. Isolated glomeruli produced 20-HETE, dihydroxyeicosatrienoic acids, and 12-hydroxyeicosatetraenoic acid (4.13 +/- 0.38, 4.20 +/- 0.38, and 2. 10 +/- 0.20 pmol. min-1. mg protein-1, respectively) when incubated with arachidonic acid (10 microM). The formation of 20-HETE was dependent on the availability of NADPH and the PO2 of the incubation medium. The formation of 20-HETE was inhibited by NO donors in a concentration-dependent manner. The production of 20-HETE was greater in glomeruli isolated from the kidneys of rats fed a low-salt diet than in kidneys of rats fed a high-salt diet (5.67 +/- 0.32 vs. 2.83 +/- 0.32 pmol. min-1. mg protein-1). Immunoblot experiments indicated that the expression of P-450 4A protein in glomeruli from the kidneys of rats fed a low-salt diet was sixfold higher than in kidneys of rats fed a high-salt diet. These results indicate that arachidonic acid is primarily metabolized to 20-HETE and dihydroxyeicosatrienoic acids in glomeruli and that glomerular P-450 activity is modulated by NO and dietary salt intake.  (+info)

*Alkane 1-monooxygenase

... alkane monooxygenase, 1-hydroxylase, AlkB, and alkane hydroxylase. It contains a diiron non-heme active site. McKenna EJ, Coon ... In enzymology, an alkane 1-monooxygenase (EC 1.14.15.3) is an enzyme that catalyzes the chemical reactions an alkane + reduced ... Alkanes of 6 to 22 carbons have been observed as substrates. This enzyme belongs to the family of oxidoreductases, specifically ... The systematic name of this enzyme class is alkane, reduced-rubredoxin:oxygen 1-oxidoreductase. Other names in common use ...

*List of MeSH codes (D08)

... camphor 5-monooxygenase MeSH D08.811.682.690.708.170.500 --- alkane 1-monooxygenase MeSH D08.811.682.690.708.170.915 --- ... 4-hydroxybenzoate 3-monooxygenase MeSH D08.811.682.690.708.557 --- kynurenine 3-monooxygenase MeSH D08.811.682.690.708.601 --- ... trans-cinnamate 4-monooxygenase MeSH D08.622.509.700 --- pepsinogen a MeSH D08.622.509.725 --- pepsinogen c MeSH D08.622. ... monophenol monooxygenase MeSH D08.811.682.690.708.170 --- cytochrome p-450 enzyme system MeSH D08.811.682.690.708.170.040 --- ...

*Rubredoxin

... alkane,reduced-rubredoxin:oxygen 1-oxidoreductase) octane + reduced rubredoxin + O2 = 1-octanol + oxidized rubredoxin + H2O EC ... 2-monooxygenase [(+)-camphor,reduced-rubredoxin:oxygen oxidoreductase (1,2-lactonizing)] (+)-bornane-2,5-dione + reduced ... rubredoxin + O2 = 5-oxo-1,2-campholide + oxidized rubredoxin + H2O EC 1.14.15.3 alkane 1-monooxygenase ( ...

*List of EC numbers (EC 1)

2-monooxygenase EC 1.14.15.3: alkane 1-monooxygenase EC 1.14.15.4: steroid 11b-monooxygenase EC 1.14.15.5: corticosterone 18- ... menthol monooxygenase EC 1.14.13.47: (S)-limonene 3-monooxygenase EC 1.14.13.48: (S)-limonene 6-monooxygenase EC 1.14.13.49: (S ... arginine 2-monooxygenase EC 1.13.12.2: lysine 2-monooxygenase EC 1.13.12.3: tryptophan 2-monooxygenase EC 1.13.12.4: lactate 2- ... steroid 17a-monooxygenase EC 1.14.99.10: steroid 21-monooxygenase EC 1.14.99.11: estradiol 6b-monooxygenase EC 1.14.99.12: 4- ...

*Desulfatibacillum alkenivorans AK-01

It has also been shown that AK-01 utilizes not only alkanes but also 1-alkenes, 1-alkanols, fatty acids and other organic acids ... but serves as a reactant in the hydroxylation of both aliphatic and aromatic hydrocarbons via monooxygenase and dioxygenase ... AK-01 is a delta-proteobacterium capable of utilizing C13-C18 alkanes as growth substrates (So et al., 1999). Analysis of ... Heat and pressure lead to the formation of a wide variety of hydrocarbons, including alkanes, alkenes, and cyclic/polycyclic ...

*Methane monooxygenase

... , or MMO, is an enzyme capable of oxidizing the C-H bond in methane as well as other alkanes. Methane ... The particulate methane monooxygenase and related ammonia monooxygenase are integral membrane proteins, occurring in ... "Evidence that particulate methane monooxygenase and ammonia monooxygenase may be evolutionarily related". FEMS Microbiol. Lett ... This is a classic monooxygenase reaction in which two reducing equivalents from NAD(P)H are utilized to split the O-O bond of ...

*Shilov system

The transformation can also performed biologically by methane monooxygenase. Overall Transformation RH + H2O + [PtCl6]2− → ROH ... and the nucleophilic oxidation of the alkane substrate. An equivalent transformation is performed industrially by steam ... 1] and [2] ). Such premature oxidation shuts down the catalysis. Finally the PtIV-CH2R undergoes nucleophilic attack by OH− or ... species over the initial PtII species since PtIV complexes will not electrophilically activate a C-H bond of the alkane ( ...

*Ammonia monooxygenase

Rasche, M.E.; Hicks, R.E.; Hyman, M.R.; Arp, D.J. (1990). "Oxidation of monohalogenated ethanes and n-chlorinated alkanes by ... "Evidence that particulate methane monooxygenase and ammonia monooxygenase may be evolutionarily related". FEMS Microbiol. Lett ... Ammonia monooxygenase (EC 1.14.99.39, AMO) is an enzyme, which catalyses the following chemical reaction ammonia + AH2 + O2 ... Zahn, J.A.; Arciero, D.M.; Hooper, A.B.; DiSpirito, A.A. (1996). "Evidence for an iron center in the ammonia monooxygenase from ...

*Cometabolism

Another example is Mycobacterium vaccae, which uses an alkane monooxygenase enzyme to oxidize propane. Accidentally, this ... OX1 can degrade PCE under aerobic conditions by using toluene-o-xylene monooxygenase (ToMO), an enzyme they produce to derive ... Several aerobic microorganisms have been demonstrated to be capable of doing this, including n-alkane, aromatic compound (e.g. ... These bacteria degrade their growth-substrate methane with the enzyme methane monooxygenase(MMO). MMO was discovered to be ...

*Alcanivorax

The aerobic metabolism of alkanes is carried out through the terminal alkane oxidation pathway, where monooxygenases initiate ... borkumensis can consume a wider variety of alkanes than other known species. A. borkumensis primarily uses alkanes as its ... The A. borkumensis genome has many sequences that each code for a different type of alkane, allowing it to be highly adaptable ... Whereas most organisms use sugars or amino acids for their source of carbon/energy, A. borkumensis uses alkanes, a type of ...

*Mycoremediation

Averaging across all studied species, 98.1%, 48.6%, and 76.4% of the initial Bunker C C10 alkane, C14 alkane, and phenanthrene ... certain fungi possess intracellular networks which constitute the xenome, consisting of cytochrome (CYP) P450 monooxygenases ... The subphylum Saccharomycotina mostly consists of yeasts and includes degraders of n-alkanes, n-alkylbenzenes, crude oil, the ... 198 (Pt 2): 1-11. doi:10.1016/j.jenvman.2017.05.010. ISSN 1095-8630. When this wastewater was supplemented with 0.1 mM glucose ...

*Methylobacillus flagellatus

... or methane monooxygenase (MMO), which degrade various environmental pollutants (i.e.: alkanes, alkenes, and mono- and poly- ... encoding a subunit of particulate methane monooxygenase), and fae (encoding formaldehyde activating enzyme) genes." The data ... 1. p. 233-238. Baev M V, Chistoserdova L V, Polanuer B M, et al. "Effect of formaldehyde on growth of obligate methylotroph ... p. 1-10. Marchenko GN, Marchenko ND, Tsygankov YD, Chistoserdov AY. "Organization of threonine biosynthesis genes from the ...
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You could search by clone name, gene name, function, source, as well as NCBI accession number. (PGEC C1-C3000, SALK C3001-C6000, STAN C6001-C9000) and SSP number (PGEC R/U90001-R/U12000, SALK R/U12001-R/U15000, STAN R/U15001-R/U18000 ...
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For the first step in the long-chain alkane degradation pathway ladA was implemented [2]; A flavoprotein alkane monooxygenase native to Geobacillus thermodinitrificans NG-80-2. It has been found to specifically oxidize the terminal regions of alkanes ranging from C15 up to at least C36. The product is the corresponding primary alkanol. LadA forms a catalytic complex with flavin mononucleotide (FMN) which utilizes dioxygen to insert an oxygen atom into the substrate. The general catalytic function involves three chemical processes: ...
The Alkanivore E.coli strain has been designed to carry the genes required for the conversion of medium (C5-C13) and long chain (C15-C36) alkanes. A general scheme for the oxidation of the alkanes is illustrated in figure 1. To create the alkane degradation constructs a number of genes encoding for alkane degradation enzymes were synthesized and combined with promoters and ribosome binding sites obtained from the BioBrick distribution plates. Combination of these genes resulted in the following BioBrick constructs (the intermediates have also been submitted to the registry). ...
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Definition of Alkane 1-monooxygenase with photos and pictures, translations, sample usage, and additional links for more information.
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The compounds [Cp(CO)3W{(CH2)nX}] (X = Br, I; n = 3 - 6) were prepared in high yield by the reaction ofNa[Cp(CO)3W ] with Br(CH2)nBr. The bromoalkyl compounds were subsequently reacted with NaI to give the corresponding ...
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Pseudomonas putida GPo1 alkane hydroxylase (AlkB) is an integral membrane protein that catalyses the hydroxylation of medium-chain alkanes (C3-C12). 1-Octyne irreversibly inhibits this non-haem di-iron mono-oxygenase under turnover conditions, suggesting that it acts as a mechanism-based inactivator. Upon binding to the active site, 1-octyne is postulated to be oxidized to an oxirene that rapidly rearranges to a reactive ketene which covalently acylates nearby residues, resulting in enzyme inactivation. In analysis of inactivated AlkB by LC-MS/MS, several residues exhibited a mass increase of 126.1 Da, corresponding to the octanoyl moiety derived from oxidative activation of 1-octyne. Mutagenesis studies of conserved acylated residues showed that Lys18 plays a critical role in enzyme function, as a single-point mutation of Lys18 to alanine (K18A) completely abolished enzymatic activity. Finally, we present a computational 3D model structure of the transmembrane domain of AlkB, which revealed the ...
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Since 2014 Alkane has sponsored the Tomingley Gold Project Community Fund, which aims to foster close relations between the TGO team and the local Narromine Shire community by providing support for local infrastructure and events. Funding is awarded twice a year on a proposals basis. To find out more about how to apply for the Tomingley Gold Project Community Fund click here.. Listening and sharing - Alkane values strong and positive relationships with local communities. We are committed to clear and regular communications about our operations and development activities, and welcome community members to engage with us directly about any of our projects.. Community newsletters - We publish two quarterly newsletters created especially for the communities interested in the Dubbo Project and Tomingley Gold Operations. They focus on development and operations updates, news of interest to residents and other community-relevant announcements. ...
Lauric acid is a saturated fat found in vegetables that provides a multitude of health benefits, such as helping to treat viral infections, according to WebMD. It is also used to make vegetable...
90622-49-4 - Alkanes, C10-32, chlorosulfonated, reaction products with ammonia - Searchable synonyms, formulas, resource links, and other chemical information.
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... ; (5Z,8Z,13E)-(15S)-11,12-Epoxy-15-hydroxyeicosa-5,8,13-trienoic acid; (5Z,8Z,13E)-(15S)-11,12-Epoxy-15-hydroxyicosa-5,8,13-trienoic acid; 15-Hydroxy-11,12-epoxyeicosatrienoic acid; C14781 ...
In the new practice depth paper (the one on the google drive link) question 5c where it asks you to identify the molecular formula of alkane X, I identified that the alkane has 7 carbon atoms in it so assumed its formula was C7H16 due to this being the general formula of alkanes. Yet the answer was C7H14, why is this ...
In butane it is the rotation about the C2-C3 bond that is of most interest since the relative position of the two methyl groups is important. This can be seen most easily by rotating the molecule to view it down the C2-C3 bond. The more important conformations are shown below ...
Cycloalkanes are types of alkanes that have one or more rings of carbon atoms in their structure. The physical properties of cycloalkanes are similar to those of alkanes, but they have higher boiling …
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0004] Provided herein are recombinant phototrophic microorganisms, comprising one or more alkane oxidation genes whose expression results in oxidation of alkanes and assimilation of the resulting products into the central metabolic pathways in phototrophic organisms such as cyanobacteria. The one or more alkane oxidation genes can be an alkane monooxygenase, an alcohol dehydrogenase or an aldehyde assimilatory gene. The recombinant photosynthetic organism converts the entire feed of alkane into the targeted product because it uses sunlight to provide energy and oxygen needed for oxidation of alkanes. Having the ability to couple oxidation of alkanes such as methane with sunlight in the recombinant phototrophic organism and energy can allow molecules of interest (e.g., butanol) to be produced biologically from natural gas in an efficient and cost effective manner. Because the recombinant phototrophic organism converts alkanes into metabolic products that are natively part of central metabolic ...
Pseudomonas oleovorans ATCC ® 8062™ Designation: TypeStrain=True Application: Assay of antimicrobial preservatives Bioresistance testing
22 20-hydroxyeicosatetraenoic acid (20-HETE) is the principal arachidonic acid metabolite in tubular and vascular structures of the rat kidney. In the tubules it inhibits sodium reabsorption, while in the renal microcirculation it is a vasoconstrictor and a regulator of myogenic tone. 20-HETE synthesis is catalyzed by the cytochrome P450 (CYP) 4A isoforms (4A1, 4A2, 4A3 and 4A8). Our studies indicated that despite the high homology, these isoforms display distinct catalytic properties. CYP4A1 is the low Km isoform and thus, by far, the most efficient 20-HETE synthesizing enzyme. Whereas CYP4A1 is solely an ω-hydroxylase, CYP4A2 and CYP4A3 also catalyze arachidonate 11,12-epoxidation. Systemic administration of CYP4A1 antisense oligodeoxynucleotides reduced the level of CYP4A proteins and 20-HETE synthesis in renal vessels by 50%, and decreased blood pressure in SHR from 137±3 to 121±4 mmHg (p , 0.05). Immunoblot analysis and inhibitor studies indicated that within the renal vasculature CYP4A1 ...
Advances in fossil fuel exploration have continued to drive availability of lower alkane feedstocks for the chemical industry. Lower alkanes are potential precursors to the plethora of basic organic chemicals. However, conversion of lower alkanes to valuable chemicals often involves indirect or multi-step reaction routes. Developing direct routes to obtain key organic chemicals from lower alkanes would benefit industry. Dehydrogenation of C2 and C3 alkanes are particularly of interest as alternatives to steam cracking and fluid catalytic cracking for obtaining C2 and C3 alkenes. This review highlights developments in non-oxidative, autothermal and oxidative dehydrogenation of C2 and C3 alkanes.We examine reaction routes to dehydrogenation of lower alkenes, and analyze the C-H activation mechanismof commercial catalysts in order to gain insight into rational design of improved catalysts for C2 and C3 alkane dehydrogenation at lower temperatures.. ...
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Introduction. Developing Fuels Petrol is made up of alkanes, which are a type of hydrocarbon. These alkanes have carbon atoms connected four times to other carbon or hydrogen atoms by singular covalent bonds, and are therefore saturated. Alkanes have different structures. Some have a chain of carbons with hydrogens connected to them. They are called chain alkanes. Others have carbons interconnecting and are called branched alkanes. Petrol has short-chained alkanes and branched alkanes as these have high octane numbers. This means that, when the alkanes are put under pressure they explode smoothly and dont cause the engine to knock. If an alkane had an octane no. of 90 then it knocks the same amount as a mixture of 90% methylcyclohexane (knocks very little) and 10% n-heptane (knocks a lot). Petrol originally had mainly long chained alkanes. Therefore , as some alkanes have the same formula but different structures, they can be changed into other alkanes during isomerisation. ...read more. ...
Buy, download and read Mechanisms of Atmospheric Oxidation of the Alkanes ebook online in PDF format for iPhone, iPad, Android, Computer and Mobile readers. Author: Jack G Calvert; Richard G Derwent; John J Orlando; Geoffrey S Tyndall; Timothy J Wallington. ISBN: 9780199710881. Publisher: Oxford University Press. An international team of eminent atmospheric scientists have prepared Mechanisms of Atmospheric Oxidation of the Alkanes as an authoritative source of information on the role of alkanes in the chemist
View Notes - Chapter 3 from CHE 201 at SUNY Buffalo. Alkane Formulas All C-C single bonds Saturated with hydrogens Ratio: CnH2n+2 Alkane homologs: CH3(CH2)nCH3 Same ratio for branched
Methods and catalysts for producing alcohols, ethers, and/or alkenes from alkanes are provided. More particularly, novel caged, or encapsulated, metal oxide catalysts and processes utilizing such catalysts to convert alkanes to alcohols and/or ethers and to convert alcohols and/or ethers to alkenes are provided.
P450BM3 (CYP102A1), a fatty acid hydroxylase from Bacillus megaterium, has been extensively studied over a period of almost forty years. The enzyme has been redesigned to catalyse the oxidation of non-natural substrates as diverse as pharmaceuticals, terpenes and gaseous alkanes using a variety of engineering strat
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14.11 Alkane Synthesis Using Organocopper Reagents. Dr. Wolfs CHM 201 & 202. 14-53. Lithium Dialkylcuprates. Lithium dialkylcuprates are useful synthetic reagents. They are prepared from alkyllithiums and a copper(I) halide. 2RLi + Cu X. R 2 Cu Li + Li X. Slideshow 6734350 by shelly-lewis
Alkanes Chapter 1.1 Organic Chemistry The study of carbon-containing compounds and their properties What s so special about carbon? Carbon has 4 bonding electrons. Thus, it can form 4 strong covalent bonds
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20-Hydroxyeicosatetraenoic acid (20-HETE) is a metabolite of arachidonic acid. Cytochrome P450 enzymes of the 4A and 4F families catalyze the omega-hydroxylation of arachidonic acid and produce 20-HETE. 20-HETE is a potent constrictor or renal, cerebral and mesenteric arteries. The vasoconstrictor response to 20-HETE is associated with activation of protein kinase, Rho kinase and the mitogen activated protein (MAP) kinase pathway C. 20-HETE also increases intracellular Ca2+ by causing the depolarization of vascular smooth muscle membrane secondary to blocking the large-conductance Ca2+-activated K+-channels and by a direct effect on L-type Ca channels. Elevations in the production of 20-HETE mediate the myogenic response of skeletal, renal and cerebral arteries to elevations in transmural pressure. There is an important interaction between nitric oxide (NO) and the formation of 20-HETE production. NO inhibits the formation of 20-HETE formation in renal and cerebral arteries and that a fall in ...
Alcanivorax borkumensis is a marine bacterium that has ability to grow on limited substrates that mainly is alkanes. The ability to use wide range of hydrocarbons is advantage of this bacterium to other marine community bacteria. A. borkumensis have two genetic systems for alkane biodegradation. The First system is alkane hydroxylase (alk-B1and alk-B2) and the second system is cytochrome P450. Until now there is not any report on cloning of cytochrome P450 gene of A. borkumensis. In this study cytochrome P450 gene from A. borkumensis was cloned. At first P450 gene was cloned in pBluescript plasmid by blunt cloning and insertion was confirmed by colony PCR. Then P450 gene was cloned in PET-26 expression vector and finally, IPTG induced gene expression in E.coli BL-21 bacterium. It was shown that longer induction time led to more expression level. The recombinant enzyme has difference absorbance in 450 nm that confirmed the activity of this recombinant enzyme.
A series of sulfated alumina catalysts were synthesised by wet impregnation with sulfate-containing solutions. The degree of surface sulfation and corresponding surface acidity could be readily tuned by varying the molarity of impregnating solution. Strong acid treatments (,0.1 M) induced aluminium-sulfate crystallisation with a concomitant decrease in porosity and surface acidity. Platinum-doped sulfated aluminas showed enhanced activity towards methane, ethane and propane combustion. Activity scaled with the degree of accessible surface sulfate and platinum loading, however C-H bond scission appeared rate-limiting over both pure and presulfated aluminas. The magnitude of sulfate-promoted propane oxidation was greatest under heavily oxidising conditions (C3H6O2 , 120) but independent of Pt loading, confirming that support-mediated alkane activation is the dominant factor in the promotional mechanism. ...
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Alkane Exploration Ltd (ALK.AX) shares are being watched closely by traders as the Awesome Oscillator signal is revealing a downward trend building over the past five bars, signaling that market momentum is building for the name.. The Awesome Oscillator (AO), created (and aptly named) by Bill Williams, is an indicator which is able to show what is happening with driving force of the market.. The Awesome Oscillator is created using the difference between the 34-period and 5-period simple moving averages of the bars midpoints (H+L)/2. The AO is usually planned as a histogram in which bars higher than the preceding bar will be colored green. Bars lower than the preceding one will be colored red. The Awesome Oscillator was introduced by Williams in his book "New Trading Dimensions".. When applying indicators for technical analysis, traders and investors might want to look at the ATR or Average True Range. The current 14-day ATR for Alkane Exploration Ltd (ALK.AX) is currently sitting at 0.02. The ...
100684-50-2 - Sulfonic acids, C18-26-alkane, sodium salts - Searchable synonyms, formulas, resource links, and other chemical information.
... definition, the homologous series of saturated, aliphatic hydrocarbons having the general formula C n H 2n+2 , as methane, CH 4 , or ethane, C 2 H 6 . See more.
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Cossy, J. [Hrsg.] Science of Synthesis : Houben-Weyl Methods of Molecular Transformations Vol.26: Category 4, Compounds with two Carbon-Heteroatom Bonds. Ketones. Stuttgart : Thieme, ...
Hydrocarbons Hydrocarbons are compounds that only contain H and C atoms, but they can be subdivided according to the following tree diagram depending on the bond types that are present ...
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3-butyl-6-isobutyl-5-methyl-1H-pyrazin-2-one - chemical structural formula, chemical names, chemical properties, synthesis references
Gordonia sp. SoCg, a Gram positive strain able to grow on long chain n-alkanes1, possess a single copy of alkB2 gene, whose product is required for n-alkane hydroxylation3. An analysis of alkB flanking regions revealed five ORFs which were designed as orf1, rubA3, rubA4, rubB and alkU, according to the sequence 14 homology with that of known alk clusters3. In G. sp. SoCg the transcription of these genes was induced by long-chain and solid n-alkanes as revealed by quantitative RT-PCR, and the essential role of alkB in nalkane degradation was demonstrated by the construction of an alkB disruption mutant strain3. The SoCg alkB gene was successfully expressed in Streptomyces coelicolor M145 (M145-AH), and the production of 1-hexadecanol from n-hexadecane oxidation was observed3. A differential study of global gene expression of M145-AH cultures was performed, where n-hexadecane (C16) glucose (GLU) and none (NC) were provided as only carbon source, respectively. Proteomic analysis, based on 2D-DIGE ...
Introduction. PLAN I am going to investigate that if the number of carbon atoms in an alkane effect the amount of heat released. The alkanes that I will use are Pentane (C5 H12 ), Hexane (C6 H14 ), Heptane (C7 H16 ) and Nonane (C9 H26 ). The formula that I will use to find the heat released is :- Mass (g) x Specific Heat Capacity (4.2/J/g/�C) x Temperature Rise (�C) ? Mass of Fuel The variables that might effect this investigation are :- * type of flame * amount of water * length of time * thermometer not started at room temperature * amount of the alkane * size of beaker holding the alkane * height from flame to beaker Prediction Alkanes are called saturated hydrocarbons because they only have single bonds between carbon atoms and are known to be in the homologous series, individuals are known as homologs. Alkanes burn in a plentiful supply of air to release energy (this is why they are used as fuels). ...read more. Middle. - Using a clamp I will hold the brass beaker about 15cm above the ...
What is left at the end of a reaction is thus typically a mixture of product and impurities (impurities being by-products and unreacted starting materials). Achieving high percentage yields is particularly important in industries that rely on organic synthesis, such as the pharmaceutical industry. It may require tens of steps to synthesise a drug molecule - if there are ten steps, each of which has a percentage yield of 90%, the overall yield is only 35%: 90% × 90% × 90% × 90% × 90% × 90% × 90% × 90% × 90% × 90% = (90%)10 = 35% ...
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Alkane series definition, the homologous series of saturated, aliphatic hydrocarbons having the general formula C n H 2n+2 , as methane, CH 4 , or ethane, C 2 H 6 . See more.
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Yes Chromium oxide reaction is valid for all alkanes. In fact, many metal (III) oxides are capable of producing such reactions on specific compounds. Examples are Aluminum and Iron (III) oxide ...
We have prepared and evaluated 2,3-epoxypropyl trialkyl substituted acetate (OXI; alpha, alpha-branched alkane carboxylic glycidyl esters) based on bran...
1) When organic acids are subjected to continuing increases in temperature, and depending on the acid itself, is it reasonable to assume that organic acids convert first to aldehydes, then to alcohols, then to alkanes given.
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Alkane Resources Ltd is an Australian multi-commodity mining and exploration company with a focus on technology metals essential for a growing range of future industries and sustainable technologies . including clean energy, electric vehicles, artificial intelligence and modern healthcare. Our projects and operations are located in Central Western New South Wales, eastern Australia.
With a Class 8 ISX12 G-Powered Natural Gas Truck Later in the Year. South Carolinas Alkane Truck is introducing new Class 7 dedicated-propane autogas cabover trucks with Ford V-10 engines modified with engine systems from Bi-Phase Technologies. A natural gas-fueled Class 8 is being readied too ...
View Notes - Practice_Problems_1_answer_key from CHM 201 at Cal Poly Pomona. others may exist others may exist 3) Give the IUPAC name for the following alkanes. Cl Cl a) b)
Free Notes on Alkanes, Alkenes and Alkynes - KCET Engineering. Read all the Important points and Chapterwise Notes on KCET Engineering
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Phonon-glass electron-crystal (PGEC) behaviour is realised in La0.5Na0.5Ti1−xNbxO3 thermoelectric oxides. The vibrational disorder imposed by the presence of both La3+ and Na+ cations on the A site of the ABO3 perovskite oxide La0.5Na0.5TiO3 produces a phonon-glass with a thermal conductivity, κ, 80% lower t 2017 Energy and Environmental Science HOT articles
The invention relates to a material which is suited as a carrier for catalysts in the dehydrogenation of alkanes and in the oxidative dehydrogenation of alkanes and which is made of an oxide ceramic
© Prentice Hall 2001Chapter 23 Nomenclature International Union of Pure and Applied Chemistry (IUPAC) has established the system We will refer to names derived from this system as systematic nomenclature or IUPAC nomenclature (shown in blue) Additional names such as isopentane and neopentane are common names (shown in red) A compound may have more than one name BUT a name must specify only one compound
The possibility of predicting the parameters in HPLC by means of theoretical calculations, represents a significant step forward in the analysis of the complex mixtures of higher boiling petroleum...
Lib 153220824141414520 lib 153220824141414520 lib 153220824141414520 lib 153220824141414520. Also provides rules ringsalkanes arsenide... Blog.cz - Stačí otevřít a budeš v obraze.
నెల్లూరు: మనుబోలు మండలం బద్వేలు క్రాస్‌రోడ్డు దగ్గర కారు బోల్తా, ముగ్గురికి గాయాలు,కర్నూలు: 16 వ రోజు జగన్ ప్రజా సంకల్ప యాత్ర,రంగారెడ్డి: మైలార్‌దేవ్‌పల్లిలో కింగ్స్‌ కాలనీలో ముస్తఫా అనే వ్యక్తిపై దుండగుల కాల్పులు,కడప: జగన్ సీఎం అయితే తన ఆస్తులు పెరుగుతాయి..చంద్రబాబు సీఎంగా ఉంటే ప్రజల ఆస్తులు పెరుగుతాయి: మంత్రి సోమిరెడ్డి,సిరిసిల్ల: అన్ని గ్రామాల్లో కేసీఆర్ గ్రామీణ ప్రగతి ...
Learn more about butyl-4-2-mercapto-4-4-dimethylpyrimidin-1-4h-yl-benzoate. We enable science by offering product choice, services, process excellence and our people make it happen.
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St. Bernardus Abt 12 is a Quadrupel (Quad) style beer brewed by Brouwerij St. Bernardus NV in Watou, Belgium. 4.46 average with 9623 ratings, reviews and opinions.
St. Bernardus Abt 12 is a Quadrupel (Quad) style beer brewed by Brouwerij St. Bernardus NV in Watou, Belgium. 4.46 average with 9624 ratings, reviews and opinions.
Lactobacillus casei BL23. Lactobacillus casei BL23 is a short, Gram-positive, facultatively anaerobic, non-motile and non-spore-forming, rod-shaped (cell size range = 0.7-1.1 x 2.0-4.0 mm) member of the industrially important lactic acid bacteria.. Pseudomonas putida GPo1. Pseudomonas putida is a Gram-negative bacterium. It is a motile, nonfermentative aerobe that can utilize acetate as carbon source and ammonium sulphate as nitrogen source. Pseudomonas is capable to form PHA granules (bioplastic). It is the host strain used for the PHASTAG Technology.. ...
Les activités liées à lindustrie du pétrole conduisent souvent à la contamination aux hydrocarbures des sols et de leau. Pendant de nombreuses années, des méthodes mécaniques et chimiques ont été le seul moyen pour éliminer les hydrocarbures provenant de sites contaminés. Puisque ces méthodes ont souvent une efficacité limitée et peuvent être très coûteux, lobjectif est de les remplacer par des méthodes plus efficaces et plus rentables, tels que la dégradation microbienne de micro-organismes indigènes dans leau et le sol capables de dégrader les contaminants dhydrocarbures. Dans ce travail, métagénome de sédiments marins huile polluée a été analysée en utilisant des méthodes bioinformatiques dans le but dévaluer la biopotentiel des communautés microbiennes pour lassainissement de la contamination aux hydrocarbures. Les cibles étaient des enzymes impliquées dans la dégradation aérobie des alcanes à court et à longue chaîne ainsi que des enzymes pour ...
In the present study, we have provided evidence that although CYP4A11 functions as a 20-HETE synthase, CYP4A22 does not contribute to renal 20-HETE biosynthesis. Furthermore, to determine the relevance of genetic variation in CYP4A11 to hypertension, we identified a coding polymorphism (T8590C), biochemically characterized 8590C as a loss-of-function variant, and demonstrated its association with hypertension in whites. Initial findings of associations between biologically plausible functional variants are often not replicated in follow-up studies, and even when associations are replicated, they are often less strong than in the initial validation cohort.27 For this reason, we conducted a study of replication in a population-based cohort of whites. Our results suggest the existence of a modest association finding in the Framingham cohort. Clearly, our findings strongly support further study of the relationship of variants in this gene with hypertension in other populations.. Both animal14,16 and ...
The analysis of catalytic partial oxidation of light alkanes indicates that processes involving this group proceed via the formation and consecutive transformations of free radicals. Depending on the properties of the catalytic system and reaction conditions the same primary radical can give different final products, olefins, oxygenated organic substances and carbon oxides. An approach to design a complex catalytic system for efficient alkane partial oxidation based on separation of zones where free radicals are formed and where they are transformed into desired products is suggested and examples of its implementation are presented.
Straight-Chain and Branched Alkanes - Chemistry LibreTexts Straight-chain hydrocarbon , Britannica.com hydrocarbons. in hydrocarbon: Physical properties. For a given number of carbon atoms, an unbranched alkane has a higher boiling point than any of its branchedchain isomers. This effect is evident upon comparing the boiling points (bp) of selected C 8H 18 isomers. Straight-Chain and … ...
Abstract We examined physiological adaptations which allow the psychrotroph Rhodococcus sp. strain Q15 to assimilate alkanes at a low temperature (alkanes are contaminants which ar..
... is a kind of medium chain fatty acid and it is constructed by 12-carbon atom chain with the name dodecanoic acid, white in colour,.... Go to product ...
Researchers in China are proposing new pathways for the CO2 catalytic conversion of oleic acid into C8-C15 alkanes. The yield of C8-C15 products reaches 73.10 mol% in a CO2 atmosphere-much higher than the 49.67 mol% yield obtained in an H2 atmosphere. In the the absence of an external H2 source,...
It looks pretty good. I am not nearly as excited as I was when the trailer for Twilight Princess came out, but since it is the only video game that I actually play, much to my Rockband-playing-friends chagrin, I cannot help but be a little stoked about it. Of, course it just dawned on me that I havent started to play Spirit Tracks, which I got for Grismas. In my defense, I have been a little preoccupied with moving, and the City Wire gig, and work. But now that I am back in school, I am playing a lot more Gamecube in an attempt to avoid doing homework or studying, so maybe Ill charge up the ol DS and kick the crap out of some Ganon or whoever the bad guy is in this one.. Remember all of that stuff about me being smart in chemistry? Scratch that. I am back to having to work at it. Yeah, yeah, polyatomic ions, I got that. Functional groups in hydrocarbons? Holy crap. I have a quiz this morning over 14 functional groups (anything that is not a single bond between a hydrogen and a carbon) that ...
hapter 10 Introduction to Organic hemistry: Alkanes 1 Organic hemistry An organic compound is a compound made from carbon atoms has one or more atoms has many atoms may also contain O, S, N, and halogens
Physiological adaptations involved in alkane assimilation at a low temperature by Rhodococcus sp. strain Q15.: We examined physiological adaptations which allow
Straight-Chain and Branched Alkanes - Chemistry LibreTexts Straight-chain hydrocarbon , Britannica.com hydrocarbons. in hydrocarbon: Physical properties. For a given number of carbon atoms, an unbranched alkane has a higher boiling point than any of its branchedchain isomers. This effect is evident upon comparing the boiling points (bp) of selected C 8H 18 isomers. Straight-Chain and … ...
Recent studies have indicated that renal arteries can produce 20-hydroxyeicosatetraenoic acid (20-HETE) and suggest the potential involvement of a P450 metabolite of arachidonic acid in the myogenic activation of canine renal arteries. In the present study, the effects of 20-HETE on isolated canine renal arcuate arteries were studied. Administration of 20-HETE to the bath or the lumen at concentrations of 0.01-1 microM produced a graded reduction in the diameter of these vessels. In contrast, 19(R)-HETE was a vasodilator, whereas 19(S)-HETE was relatively inactive. The vasoconstrictor response to 20-HETE was not altered by the cyclooxygenase inhibitor indomethacin, endoperoxide/thromboxane receptor antagonist SQ29548, or combined blockade of the cyclooxygenase, lipoxygenase, and P450 pathways using indomethacin, baicalein, and 7-ethoxyresorufin. The response to 20-HETE was associated with depolarization and a sustained increase in the intracellular calcium concentration in renal vascular smooth ...
It is of great importance to study the selective oxidation of light alkanes on metal oxide catalysts from a fundamental and industrial point of view. Clarification of the relationship between the structure of the oxide catalysts and their catalytic properties is vital for the design of efficient catalysts and for the realization of efficient oxidative conversion of light alkanes. In this review, we summarize some recent progress in the understanding of the structures and catalytic properties of several catalysts including V-P-O, V-Sb-O, Mo-V-Te-Nb-O, and V-Mg-O metal oxides. These oxides as well as the Re-Sb-O catalysts developed by us have been extensively studied for the selective oxidation of light alkanes. We then revisit several key aspects including multi-functionality, site isolation, and phase cooperation. These aspects are required for catalysts that are efficient in the selective oxidation of light alkanes and will provide insights into the design and synthesis of metal oxide catalysts ...
The dried aerial parts of scutellaria lateriflora L. (family Labiatae), have been used for many years as a domestic remedy for epilepsy, hysteria, and nervous tension states. Although several phytochemical investigations have been made regarding the constituents present in this species (i. e. flavonoids, iridoids), none has been recorded concerning the cuticular wax. Using thin-layer chromatography (TLC), column chromatography, gas-liquid chromatography (GLC), and GLC-mass spectrometry, laterifloral collected from Mazandaran province in northern Iran have been analysed. The wax yield was 1.2%. The alkanes fall in the range of C23 to C37 the odd carbon number n-alkanes being predominant. The concentration of branched alkane is low (1.2%). Three homologous series of branched alkanes were identified as 3,9-dimethyl alkanes, 2-methyl alkanes and 3-methyl alkanes.

Alkane 1-monooxygenase - WikipediaAlkane 1-monooxygenase - Wikipedia

... alkane monooxygenase, 1-hydroxylase, AlkB, and alkane hydroxylase. It contains a diiron non-heme active site. McKenna EJ, Coon ... In enzymology, an alkane 1-monooxygenase (EC 1.14.15.3) is an enzyme that catalyzes the chemical reactions an alkane + reduced ... Alkanes of 6 to 22 carbons have been observed as substrates. This enzyme belongs to the family of oxidoreductases, specifically ... The systematic name of this enzyme class is alkane, reduced-rubredoxin:oxygen 1-oxidoreductase. Other names in common use ...
more infohttps://en.wikipedia.org/wiki/Alkane_1-monooxygenase

Alkane 1-monooxygenase: Definition with Alkane 1-monooxygenase Pictures and PhotosAlkane 1-monooxygenase: Definition with Alkane 1-monooxygenase Pictures and Photos

Definition of Alkane 1-monooxygenase with photos and pictures, translations, sample usage, and additional links for more ... alkane. alkane 1-monooxygenase (current term). alkane series. alkanediyl. alkanediyls. alkanes. alkanesulfonates. ... Medical Definition of Alkane 1-monooxygenase. 1. Alkb is an integral membrane protein component Registry number: EC 1.14.15.3 ... Alkane 1-monooxygenase Pictures. Click the following link to bring up a new window with an automated collection of images ...
more infohttp://www.lexic.us/definition-of/alkane_1-monooxygenase

Springer Handbook of Enzymes | SpringerLinkSpringer Handbook of Enzymes | SpringerLink

2-monooxygenase Pages 9-15 * Alkane 1-monooxygenase Pages 16-25 ... Page 1 Navigate to page number. of 3 Next Navigate to page ...
more infohttps://link.springer.com/book/10.1007/3-540-30439-8

Cyp4a10 - Cytochrome P450 4A10 - Mus musculus (Mouse) - Cyp4a10 gene & proteinCyp4a10 - Cytochrome P450 4A10 - Mus musculus (Mouse) - Cyp4a10 gene & protein

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH- ... leukotriene-B4 20-monooxygenase activity Source: MGI. *monooxygenase activity Source: UniProtKB ,p>Traceable Author Statement,/ ... alkane 1-monooxygenase activity Source: MGI. *arachidonic acid epoxygenase activity Source: MGI ... Long-chain fatty acid omega-monooxygenase (EC:1.14.14.80By similarity. Manual assertion inferred from sequence similarity toi ...
more infohttps://www.uniprot.org/uniprot/O88833

Team:TU Delft/project/hydrocarbon tolerance - 2010.igem.orgTeam:TU Delft/project/hydrocarbon tolerance - 2010.igem.org

style=color:black; background-color:white; cellpadding=5 cellspacing=0 border=1 ... Alkane 1-monooxygenase (Gordonia sp. TF6). -. ,-. -. ,[http://partsregistry.org/Part:BBa_B0015 B0015]. ...
more infohttp://2010.igem.org/wiki/index.php?title=Team:TU_Delft/project/hydrocarbon_tolerance&diff=100864&oldid=49569

Team:TU Delft/project/hydrocarbon tolerance - 2010.igem.orgTeam:TU Delft/project/hydrocarbon tolerance - 2010.igem.org

style=color:black; background-color:white; cellpadding=5 cellspacing=0 border=1 ... Alkane 1-monooxygenase (Gordonia sp. TF6). -. ,-. -. ,[http://partsregistry.org/Part:BBa_B0015 B0015]. ...
more infohttp://2010.igem.org/wiki/index.php?title=Team:TU_Delft/project/hydrocarbon_tolerance&diff=cur&oldid=49603

Burkholderia cepacia (Palleroni and Holmes) Yabuuchi et al. ATCC ®Burkholderia cepacia (Palleroni and Holmes) Yabuuchi et al. ATCC ®

Nucleotide (GenBank) : AJ293344 Burkholderia cepacia partial alkB gene for putative alkane 1-monooxygenase. ... Nucleotide (GenBank) : L36602 Rhizobium meliloti (clone Rhizo70-1) heat shock protein 70 (hsp70) gene, complete cds. ... Nucleotide (GenBank) : L36603 Pseudomonas cepacia (clone Psudom70-1) heat shock protein 70 (hsp70) gene, complete cds. ...
more infohttps://www.atcc.org/en/Products/Quality_Control_Strains/By_Organization__Company/25416.aspx?p=1&rel=%7B0%7D

IMET 7370 Strain Passport - StrainInfoIMET 7370 Strain Passport - StrainInfo

Gordonia bronchialis DSM 43247 putative alkane 1-monooxygenase (alkB) gene, partial cds. DSM 43247 T ... Molecular detection and phylogenetic analysis of the alkane 1-monooxygenase gene from Gordonia spp ... 43 items found, displaying 1 to 25.[First/Prev] 1, 2 [Next/Last] ... 43 items found, displaying 1 to 25.[First/Prev] 1, 2 [Next/Last ... Gordonia bronchialis DSM 43247 catechol 1,2-dioxygenase (catA) gene, partial cds. DSM 43247 T ...
more infohttp://www.straininfo.net/strains/12158

JCM 3231 Strain Passport - StrainInfoJCM 3231 Strain Passport - StrainInfo

Gordonia bronchialis DSM 43247 putative alkane 1-monooxygenase (alkB) gene, partial cds. DSM 43247 T ... Molecular detection and phylogenetic analysis of the alkane 1-monooxygenase gene from Gordonia spp ... 43 items found, displaying 1 to 25.[First/Prev] 1, 2 [Next/Last] ... 43 items found, displaying 1 to 25.[First/Prev] 1, 2 [Next/Last ... Gordonia bronchialis DSM 43247 catechol 1,2-dioxygenase (catA) gene, partial cds. DSM 43247 T ...
more infohttp://www.straininfo.net/strains/12160

Leukotriene-B(4) omega-hydroxylase elisa and antibodyLeukotriene-B(4) omega-hydroxylase elisa and antibody

20-monooxygenase 2).. CYP4F3: Cytochromes P450 are a group of heme-thiolate monooxygenases. This enzyme requires molecular ... Molecular Function: alkane 1-monooxygenase activity; arachidonic acid epoxygenase activity; leukotriene-B4 20-monooxygenase ... Cytochromes P450 are a group of heme-thiolate monooxygenases. This enzyme requires molecular oxygen and NADPH for the omega- ... EPS8-like 1 (EPS8L1) ELISA Kit. • DRD3 Antibody. • 3-nitrotyrosine (3-NT) ELISA Kit. • TRIM10 Antibody. more .... ...
more infohttps://www.mybiosource.com/protein_family.php?root=leukotriene-b-4-omega-hydroxylase

Network Portal - Gene PA4038Network Portal - Gene PA4038

4-hydroxyphenylacetate 3-monooxygenase small chain (NCBI). 192, 337. PA4187. PA4187. probable major facilitator superfamily ( ... alkane-1-monooxygenase (NCBI). 30, 192. PA3387. rhlG. beta-ketoacyl reductase (NCBI). 192, 550. ... POSITION A C G T 1 0.0 0.0 0.0 1.0 2 1.0 0.0 0.0 0.0 3 0.25 0.0 0.0 0.75 4 0.0 0.0 1.0 0.0 5 0.5 0.25 0.25 0.0 6 1.0 0.0 0.0 ... 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this ...
more infohttp://networks.systemsbiology.net/pae/gene/PA4038

Network Portal - Gene PA2651Network Portal - Gene PA2651

alkane-1-monooxygenase (NCBI). 30, 192. PA2584. pgsA. CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (NCBI) ... POSITION A C G T 1 1.0 0.0 0.0 0.0 2 1.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.0 4 1.0 0.0 0.0 0.0 5 0.0 0.0 1.0 0.0 6 0.0 0.0 0.0 1.0 7 ... POSITION A C G T 1 0.0 0.0 1.0 0.0 2 0.0 0.2 0.2 0.6 3 0.6 0.0 0.4 0.0 4 0.0 0.0 1.0 0.0 5 0.0 0.6 0.0 0.4 6 0.2 0.4 0.4 0.0 7 ... 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this ...
more infohttp://networks.systemsbiology.net/pae/gene/PA2651

Browse EndoSite | RNAi screening of S2R+ cells in Drosophila MelanogasterBrowse EndoSite | RNAi screening of S2R+ cells in Drosophila Melanogaster

There is experimental evidence that it has the molecular function: alkane 1-monooxygenase activity. There is experimental ... 1; R2: Replicate No.2; R3: Replicate No. 3. Primary screen data - CG10248: SN. RN. WN. Fint1. Fint2. Fint3. Fint4. Fnum. Fmph1 ... 1. 65. 0.049072431. -0.121728901. -0.374768319. -0.386066868. 1.233482494. 1.752211326. 1.62871669. 1.814638184. 2.696060555. ...
more infohttp://rnai.ncbs.res.in/cgi-bin/endosite/gene_page.cgi?gene_id=CG10248

The complete genomic sequence of Nocardia farcinica IFM 10152 | PNASThe complete genomic sequence of Nocardia farcinica IFM 10152 | PNAS

Regulation of the Alkane Hydroxylase CYP153 Gene in a Gram-Positive Alkane-Degrading Bacterium, Dietzia sp. Strain DQ12-45-1b ... and styrene monooxygenase (Nfa12190, Nfa32440, and Nfa32460). Of 103 oxygenases, 27 are putative cytochrome P450 monooxygenases ... Involvement of an Alkane Hydroxylase System of Gordonia sp. Strain SoCg in Degradation of Solid n-Alkanes ... Two Novel Alkane Hydroxylase-Rubredoxin Fusion Genes Isolated from a Dietzia Bacterium and the Functions of Fused Rubredoxin ...
more infohttps://www.pnas.org/content/101/41/14925?ijkey=c54739eb1195e633fa6765655ab18c566715d0f5&keytype2=tf_ipsecsha

Protocols and Video Articles Authored by Janet K. JanssonProtocols and Video Articles Authored by Janet K. Jansson

In addition, alkane biodegradation genes are described for the first time in members of Planctomycetes. ... Prospecting Biotechnologically-Relevant Monooxygenases from Cold Sediment Metagenomes: An In Silico Approach Marine Drugs. Apr ... Jan, 2016 , Pubmed ID: 26547568 We aimed to gain insight into the alkane degradation potential of microbial communities from ... A total of 6178 sequences annotated as alkane-1-monooxygenases (EC 1.14.15.3) were retrieved from a shotgun metagenomic dataset ...
more infohttps://www.jove.com/author/Janet+K._Jansson

István Máthé - ODT Personal data sheetIstván Máthé - ODT Personal data sheet

The detection and phylogenetic analysis of the alkane 1-monooxygenase gene of members of the genus Rhodococcus, SYSTEMATIC AND ... 1-7.. type of document: Journal paper/Article. number of independent citations: 2. language: English. DOI ... Part 1) pp. 298-301.. type of document: Journal paper/Article. number of independent citations: 10. language: English. DOI ... 1) pp. 87-98.. type of document: Journal paper/Article. number of independent citations: 6. language: English. DOI ...
more infohttps://doktori.hu/index.php?menuid=192&lang=EN&sz_ID=23959

Detection of Genes Involved in Biodegradation and Biotransformation in Microbial Communities by Using 50-Mer Oligonucleotide...Detection of Genes Involved in Biodegradation and Biotransformation in Microbial Communities by Using 50-Mer Oligonucleotide...

... and alkane monooxygenase) (3, 31, 34, 50). Thus, the developed microarray should be useful for assessing possible horizontal ... Bacterial strains, growth conditions, and soil samples.Pseudomonas putida Gpo1 (γ-proteobacterium; aerobic alkane degrader) and ... In addition, 4-chlorobenzate CoA ligase, alkane-1 monooxygenase, benzoyl CoA ligase, and cis-naphthalene dihydrodiol ... whereas very strong hybridization was observed for the alkane 1-monooxygenase gene (gi5824143) when the probe was 94% similar. ...
more infohttps://aem.asm.org/content/70/7/4303?ijkey=417aa8bf60864ca922e6cc02bb0221c3526c884c&keytype2=tf_ipsecsha

Journal: Journal of biotechnology / Publication Year: 2016 / Source: 2016 v.226 - PubAg Search ResultsJournal: Journal of biotechnology / Publication Year: 2016 / Source: 2016 v.226 - PubAg Search Results

In addition, several genes encoding aliphatic hydrocarbon degraders (alkane 1-monooxygenase, haloalkane deha .... DOI:. 10.1016 ... Gram-negative bacteria; aerobes; alkanes; biodegradation; cytochrome P-450; genes; nucleotide sequences; pollutants; ribosomal ... 1-7. ISSN:. 0168-1656. Subject:. Bacillus cereus; Escherichia coli; Listeria innocua; Pseudomonas fluorescens; Staphylococcus ... Process optimization and kinetic modelling of cyclic (1→3, 1→6)-β-glucans production from Bradyrhizobium japonicum MTCC120 ...
more infohttps://pubag.nal.usda.gov/?f%5Bjournal_name%5D%5B%5D=Journal+of+biotechnology&f%5Bpublication_year_rev%5D%5B%5D=7984-2016&f%5Bsource%5D%5B%5D=2016+v.226

List of MeSH codes (D08) - WikipediaList of MeSH codes (D08) - Wikipedia

... camphor 5-monooxygenase MeSH D08.811.682.690.708.170.500 --- alkane 1-monooxygenase MeSH D08.811.682.690.708.170.915 --- ... 4-hydroxybenzoate 3-monooxygenase MeSH D08.811.682.690.708.557 --- kynurenine 3-monooxygenase MeSH D08.811.682.690.708.601 --- ... trans-cinnamate 4-monooxygenase MeSH D08.622.509.700 --- pepsinogen a MeSH D08.622.509.725 --- pepsinogen c MeSH D08.622. ... monophenol monooxygenase MeSH D08.811.682.690.708.170 --- cytochrome p-450 enzyme system MeSH D08.811.682.690.708.170.040 --- ...
more infohttps://en.wikipedia.org/wiki/List_of_MeSH_codes_(D08)

KEGG BRITE: KEGG Orthology (KO) - Acinetobacter baumannii AYEKEGG BRITE: KEGG Orthology (KO) - Acinetobacter baumannii AYE

ABAYE2014 alkM; terminal alkane-1-monooxygenase ABAYE2843 putative ferredoxin reductase component (dioxygenase) ABAYE1067 ... alkane 1-monooxygenase [EC:1.14.15.3] K00529 hcaD; 3-phenylpropionate/trans-cinnamate dioxygenase ferredoxin reductase ... K01810 GPI; glucose-6-phosphate isomerase [EC:5.3.1.9] K03841 FBP; fructose-1,6-bisphosphatase I [EC:3.1.3.11] K01624 FBA; ... K00012 UGDH; UDPglucose 6-dehydrogenase [EC:1.1.1.22] K00064 E1.1.1.122; D-threo-aldose 1-dehydrogenase [EC:1.1.1.122] K00128 ...
more infohttp://www.genome.jp/kegg-bin/get_htext?aby00001+ABAYE2333

KEGG BRITE: KEGG Orthology (KO) - Mycobacterium tuberculosis H37RvKEGG BRITE: KEGG Orthology (KO) - Mycobacterium tuberculosis H37Rv

Rv3252c alkB; transmembrane alkane 1-monooxygenase AlkB Rv1869c reductase Rv0761c adhB; alcohol dehydrogenase B Rv3086 adhD; ... alkane 1-monooxygenase [EC:1.14.15.3] K00529 hcaD; 3-phenylpropionate/trans-cinnamate dioxygenase ferredoxin reductase ... K01579 panD; aspartate 1-decarboxylase [EC:4.1.1.11] K01580 E4.1.1.15; glutamate decarboxylase [EC:4.1.1.15] K07250 gabT; 4- ... Rv3601c panD; aspartate 1-decarboxylase Rv3432c gadB; glutamate decarboxylase GadB Rv2589 gabT; 4-aminobutyrate ...
more infohttp://www.genome.jp/kegg-bin/get_htext?mtu00001+Rv0131c

BRENDA - Search ResultBRENDA - Search Result

long-chain alkane monooxygenase. environmental protection. the thermophilic soluble monomeric LadA is an ideal candidate for ... LinB catalyses the conversion of a broad range of halogenated alkanes to their corresponding alcohols which makes it of ... the strain WBC3, also possessing 4-nitrophenyl 4-monooxygenase activity through PnpA, has a potential in bioremediation of the ... Escherichia coli harboring the DMS monooxygenase genes from strain 20B alone, or in combination with the cumene dioxygenase ...
more infohttp://www.brenda-enzymes.org/search_result.php?a=2&W%5B2%5D=environmental%20protection&T%5B2%5D=2&Search=Search&l=100&os=1&RNV=1&RN=&T%5B0%5D=2&W%5B1%5D=&T%5B1%5D=2&V%5B3%5D=1&W%5B3%5D=&T%5B3%5D=2&W%5B5%5D=&T%5B5%5D=2&V%5B6%5D=1&W%5B6%5D=&T%5B6%5D=2

Peroxisomal and microsomal fatty acid oxidation in liver of rats after chronic ethanol consumption. - Semantic ScholarPeroxisomal and microsomal fatty acid oxidation in liver of rats after chronic ethanol consumption. - Semantic Scholar

1. Microsomal P450 and peroxisomal fatty acid oxidation activities were studied in liver of rats after long-term ethanol ... 1. Microsomal P450 and peroxisomal fatty acid oxidation activities were studied in liver of rats after long-term ethanol ...
more infohttps://www.semanticscholar.org/paper/Peroxisomal-and-microsomal-fatty-acid-oxidation-in-Orellana-Rodrigo/e12329a85eac4da26140c6e4215ba6c0372189ee

Hepatocellular hypertrophy and cell proliferation in Sprague-Dawley rats from dietary exposure to potassium...Hepatocellular hypertrophy and cell proliferation in Sprague-Dawley rats from dietary exposure to potassium...

After treatment for 1 day, K⁺ PFOS (100 ppm), PB, and Wy 14,643 increased mean hepatic DNA concentration and total hepatic DNA ... Male Sprague-Dawley rats were fed K⁺ PFOS (20 or 100 ppm) for either 1, 7, or 28 days. Wyeth 14,643 (Wy 14,643, 50 ppm) and ... Alkane 1-monooxygenase. *Receptors, Nuclear. *Thyroid Gland Follicular Cell. *Liver diseases. *part per million (ppm) ... Male Sprague-Dawley rats were fed K⁺ PFOS (20 or 100 ppm) for either 1, 7, or 28 days. Wyeth 14,643 (Wy 14,643, 50 ppm) and ...
more infohttps://www.semanticscholar.org/paper/Hepatocellular-hypertrophy-and-cell-proliferation-Elcombe-Elcombe/5e7ae88d3419bda5287bc7df25acf3b9765199f9

Edgardo Farinas | Chemistry & Environmental ScienceEdgardo Farinas | Chemistry & Environmental Science

9. Directed Evolution of a Cytochrome P450 Monooxygenase for Alkane Oxidation. Edgardo T. Farinas, Ulrich Schwaneberg, Anton ... Directed Evolution of a Cytochrome P450 Monooxygenase for Alkane Oxidation. 07/20/01, Reference No. CIT-3514 ... "Directed Evolution of Cytochrome P450 BM3: Design of an enzymatic system to make a diol from an alkane". Edgardo Farinas and ... 4. Colorimetric Screen for Aliphatic Hydroxylation by Cytochrome P450 Using p-Nitrophenyl-Substituted Alkanes. Direct Evolution ...
more infohttps://chemistry.njit.edu/faculty/edgardo
  • U.S. Patent #9,034,629, issued on May 19, covers both the cyanobacterium and the process for directly converting CO2 into medium-chain alkanes, which are the molecular basis of diesel, jet fuel and gasoline. (blogspot.com)
  • In this study we analyzed the functional diversity of genes for alkane hydroxylases, the enzymes responsible for converting alkanes to more labile alcohols, as found in the genomes of nineteen psychrophiles for which alkane degradation has not been reported. (biomedcentral.com)
  • The chromosome of N. farcinica , unlike that of Streptomyces ( 13 , 14 ), has a circular topology ( Fig. 1 ) and encodes 53 tRNA genes, three copies of ribosomal RNA operons, and 5,674 predicted protein-coding sequences. (pnas.org)
  • Sequence analysis of 16S rRNA, gyrB and catA genes and DNA-DNA hybridization reveal that Rhodococcus jialingiae is a later synonym of Rhodococcus qingshengii , INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 64: (Part 1) pp. 298-301. (doktori.hu)
  • reported the cloning and characterization of several genes from M. austroafricanum IFP 2012 involved in the lower portion of the MTBE pathway (from 2-methyl-2-hydroxy-1-propanol to 2-hydroxyisobutyrate). (ethz.ch)
  • Such genes and enzymes can be those described in Tables 1 and 2. (blogspot.com)
  • 1. A Designed Synthetic Analogue of Co(III)-Bleomycin with Enhanced DNA-Binding and Photocleaving Activity. (njit.edu)
  • Many species of Nocardia cause the disease nocardiosis in humans and animals on lung, central nervous system, brain, and cutaneous tissues ( 1 ), and a species Nocardia asteroides is suspected to be an etiological agent of Parkinson's disease ( 2 ). (pnas.org)
  • This study developed and validated a robust high-throughput microwell platform for whole-cell two-liquid phase bio-oxidations of highly volatile n-alkanes. (biomedcentral.com)
  • Psychrophiles are presumed to play a large role in the catabolism of alkanes and other components of crude oil in natural low temperature environments. (biomedcentral.com)
  • Two-dimensional HPLC-MS/MS was employed to identify differential proteome profiles generated in response to 1 mM chromate under LB and M9L growth conditions. (biomedcentral.com)
  • Finally, we present a computational 3D model structure of the transmembrane domain of AlkB, which revealed the overall packing arrangement of the transmembrane helices within the lipid bilayer and the location of the active site mapped by the 1-octyne modifications. (biochemj.org)
  • The transformation of environmental contaminants is a complex process that is influenced by the nature and amount of the contaminant present, the structure and dynamics of the indigenous microbial community, and the interplay of geochemical and biological factors at contaminated sites ( 1 , 14 , 26 , 36 ). (asm.org)