Aliivibrio fischeri: A species of gram-negative bacteria in the genus ALIIVIBRIO, which exhibits LUMINESCENCE. A. fischeri is found in a symbiotic relationship with the SQUID Euprymna scolopes.Aliivibrio salmonicida: A species of gram-negative bacteria in the genus ALIIVIBRIO. Aliivibrio salmonicida is the causative agent of cold water vibriosis, a HEMORRHAGIC SEPTICEMIA of ATLANTIC SALMON.Aliivibrio: A genus of gram-negative, rod-shaped bacteria in the family VIBRIONACEAE. They are found in the marine environment often associated with animals.Aliivibrio Infections: Infections with bacteria of the genus ALIIVIBRIO.Vibrionaceae: A family of gram-negative bacteria whose members predominate in the bacterial flora of PLANKTON; FISHES; and SEAWATER. Some members are important pathogens for humans and animals.Vibrio: A genus of VIBRIONACEAE, made up of short, slightly curved, motile, gram-negative rods. Various species produce cholera and other gastrointestinal disorders as well as abortion in sheep and cattle.Decapodiformes: A superorder of CEPHALOPODS comprised of squid, cuttlefish, and their relatives. Their distinguishing feature is the modification of their fourth pair of arms into tentacles, resulting in 10 limbs.Gadiformes: An order of fish including the families Gadidae (cods), Macrouridae (grenadiers), and hakes. The large Gadidae family includes cod, haddock, whiting, and pollock.Symbiosis: The relationship between two different species of organisms that are interdependent; each gains benefits from the other or a relationship between different species where both of the organisms in question benefit from the presence of the other.Animal Structures: Organs and other anatomical structures of non-human vertebrate and invertebrate animals.Luminescent Measurements: Techniques used for determining the values of photometric parameters of light resulting from LUMINESCENCE.Luminescence: Emission of LIGHT when ELECTRONS return to the electronic ground state from an excited state and lose the energy as PHOTONS. It is sometimes called cool light in contrast to INCANDESCENCE. LUMINESCENT MEASUREMENTS take advantage of this type of light emitted from LUMINESCENT AGENTS.Nucleoside-Phosphate Kinase: An enzyme that catalyzes reversible reactions of a nucleoside triphosphate, e.g., ATP, with a nucleoside monophosphate, e.g., UMP, to form ADP and UDP. Many nucleoside monophosphates can act as acceptor while many ribo- and deoxyribonucleoside triphosphates can act as donor. EC 2.7.4.4.Bivalvia: A class in the phylum MOLLUSCA comprised of mussels; clams; OYSTERS; COCKLES; and SCALLOPS. They are characterized by a bilaterally symmetrical hinged shell and a muscular foot used for burrowing and anchoring.4-Butyrolactone: One of the FURANS with a carbonyl thereby forming a cyclic lactone. It is an endogenous compound made from gamma-aminobutyrate and is the precursor of gamma-hydroxybutyrate. It is also used as a pharmacological agent and solvent.Bacterial Proteins: Proteins found in any species of bacterium.Neosartorya: A genus of ascomycetous fungi in the family Trichocomaceae, order EUROTIALES. Some species can cause opportunistic infections in humans, similar to its anamorph ASPERGILLUS.Rec A Recombinases: A family of recombinases initially identified in BACTERIA. They catalyze the ATP-driven exchange of DNA strands in GENETIC RECOMBINATION. The product of the reaction consists of a duplex and a displaced single-stranded loop, which has the shape of the letter D and is therefore called a D-loop structure.Photobacterium: A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria that are common in the marine environment and on the surfaces and in the intestinal contents of marine animals. Some species are bioluminescent and are found as symbionts in specialized luminous organs of fish.DNA, Ribosomal: DNA sequences encoding RIBOSOMAL RNA and the segments of DNA separating the individual ribosomal RNA genes, referred to as RIBOSOMAL SPACER DNA.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Quorum Sensing: A phenomenon where microorganisms communicate and coordinate their behavior by the accumulation of signaling molecules. A reaction occurs when a substance accumulates to a sufficient concentration. This is most commonly seen in bacteria.Phylogeny: The relationships of groups of organisms as reflected by their genetic makeup.DNA-Directed RNA Polymerases: Enzymes that catalyze DNA template-directed extension of the 3'-end of an RNA strand one nucleotide at a time. They can initiate a chain de novo. In eukaryotes, three forms of the enzyme have been distinguished on the basis of sensitivity to alpha-amanitin, and the type of RNA synthesized. (From Enzyme Nomenclature, 1992).Gene Expression Regulation, Bacterial: Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.RNA, Ribosomal, 16S: Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.

Microbial factor-mediated development in a host-bacterial mutualism. (1/147)

Tracheal cytotoxin (TCT), a fragment of the bacterial surface molecule peptidoglycan (PGN), is the factor responsible for the extensive tissue damage characteristic of whooping cough and gonorrhea infections. Here, we report that Vibrio fischeri also releases TCT, which acts in synergy with lipopolysaccharide (LPS) to trigger tissue development in its mutualistic symbiosis with the squid Euprymna scolopes. As components of PGN and LPS have commonly been linked with pathogenesis in animals, these findings demonstrate that host interpretation of these bacterial signal molecules is context dependent. Therefore, such differences in interpretation can lead to either inflammation and disease or to the establishment of a mutually beneficial animal-microbe association.  (+info)

Complete genome sequence of Vibrio fischeri: a symbiotic bacterium with pathogenic congeners. (2/147)

Vibrio fischeri belongs to the Vibrionaceae, a large family of marine gamma-proteobacteria that includes several dozen species known to engage in a diversity of beneficial or pathogenic interactions with animal tissue. Among the small number of pathogenic Vibrio species that cause human diseases are Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus, the only members of the Vibrionaceae that have had their genome sequences reported. Nonpathogenic members of the genus Vibrio, including a number of beneficial symbionts, make up the majority of the Vibrionaceae, but none of these species has been similarly examined. Here we report the genome sequence of V. fischeri ES114, which enters into a mutualistic symbiosis in the light organ of the bobtail squid, Euprymna scolopes. Analysis of this sequence has revealed surprising parallels with V. cholerae and other pathogens.  (+info)

Magnesium promotes flagellation of Vibrio fischeri. (3/147)

The bacterium Vibrio fischeri requires bacterial motility to initiate colonization of the Hawaiian squid Euprymna scolopes. Once colonized, however, the bacterial population becomes largely unflagellated. To understand environmental influences on V. fischeri motility, we investigated migration of this organism in tryptone-based soft agar media supplemented with different salts. We found that optimal migration required divalent cations and, in particular, Mg2+. At concentrations naturally present in seawater, Mg2+ improved migration without altering the growth rate of the cells. Transmission electron microscopy and Western blot experiments suggested that Mg2+ addition enhanced flagellation, at least in part through an effect on the steady-state levels of flagellin protein.  (+info)

Vibrio fischeri uses two quorum-sensing systems for the regulation of early and late colonization factors. (4/147)

Vibrio fischeri possesses two quorum-sensing systems, ain and lux, using acyl homoserine lactones as signaling molecules. We have demonstrated previously that the ain system activates luminescence gene expression at lower cell densities than those required for lux system activation and that both systems are essential for persistent colonization of the squid host, Euprymna scolopes. Here, we asked whether the relative contributions of the two systems are also important at different colonization stages. Inactivation of ain, but not lux, quorum-sensing genes delayed initiation of the symbiotic relationship. In addition, our data suggest that lux quorum sensing is not fully active in the early stages of colonization, implying that this system is not required until later in the symbiosis. The V. fischeri luxI mutant does not express detectable light levels in symbiosis yet initiates colonization as well as the wild type, suggesting that ain quorum sensing regulates colonization factors other than luminescence. We used a recently developed V. fischeri microarray to identify genes that are controlled by ain quorum sensing and could be responsible for the initiation defect. We found 30 differentially regulated genes, including the repression of a number of motility genes. Consistent with these data, ain quorum-sensing mutants displayed an altered motility behavior in vitro. Taken together, these data suggest that the sequential activation of these two quorum-sensing systems with increasing cell density allows the specific regulation of early colonization factors (e.g., motility) by ain quorum sensing, whereas late colonization factors (e.g., luminescence) are preferentially regulated by lux quorum sensing.  (+info)

Identifying components of the NF-kappaB pathway in the beneficial Euprymna scolopes-Vibrio fischeri light organ symbiosis. (5/147)

The Toll/NF-kappaB pathway is a common, evolutionarily conserved innate immune pathway that modulates the responses of animal cells to microbe-associated molecular patterns (MAMPs). Because MAMPs have been implicated as critical elements in the signaling of symbiont-induced development, an expressed sequence tag library from the juvenile light organ of Euprymna scolopes was used to identify members of the Toll/NF-kappaB pathway. Full-length transcripts were identified by using 5' and 3' RACE PCR. Seven transcripts critical for MAMP-induced triggering of the Toll/NF-kappaB phosphorylation cascade have been identified, including receptors, signal transducers, and a transcription factor. Further investigations should elucidate the role of the Toll/NF-kappaB pathway in the initiation of the beneficial symbiosis between E. scolopes and Vibrio fischeri.  (+info)

The LuxR receptor: the sites of interaction with quorum-sensing signals and inhibitors. (6/147)

The function of LuxR homologues as quorum sensors is mediated by the binding of N-acyl-L-homoserine lactone (AHL) signal molecules to the N-terminal receptor site of the proteins. In this study, site-directed mutagenesis was carried out of the amino acid residues comprising the receptor site of LuxR from Vibrio fischeri, and the ability of the L42A, L42S, Y62F, W66F, D79N, W94D, V109D, V109T and M135A LuxR mutant proteins to activate green fluorescent protein expression from a P(luxI) promoter was measured. X-ray crystallographic studies of the LuxR homologue TraR indicated that residues Y53 and W57 form hydrogen bonds to the 1-carbonyl group and the ring carbonyl group, respectively, of the cognate AHL signal. Based on the activity and signal specificity of the LuxR mutant proteins, and on molecular modelling, a model is suggested in which Y62 (corresponding to Y53 in TraR) forms a hydrogen bond with the ring carbonyl group rather than the 1-carbonyl group, while W66 (corresponding to W57 in TraR) forms a hydrogen bond to the 1-carbonyl group. This flips the position of the acyl side chain in the LuxR/signal molecule complex compared to the TraR/signal molecule complex. Halogenated furanones from the marine alga Delisea pulchra and the synthetic signal analogue N-(sulfanylacetyl)-L-homoserine lactone can block quorum sensing. The LuxR mutant proteins were insensitive to inhibition by N-(propylsulfanylacetyl)-L-homoserine lactone. In contrast, the mutations had only a minor effect on the sensitivity of the proteins to halogenated furanones, and the data strongly suggest that these compounds do not compete in a 'classic' way with N-3-oxohexanoyl-L-homoserine lactone for the binding site. Based on modelling and experimental data it is suggested that these compounds bind in a non-agonist fashion.  (+info)

New rfp- and pES213-derived tools for analyzing symbiotic Vibrio fischeri reveal patterns of infection and lux expression in situ. (7/147)

Genetically altered or tagged Vibrio fischeri strains can be observed in association with their mutualistic host Euprymna scolopes, providing powerful experimental approaches for studying this symbiosis. Two limitations to such in situ analyses are the lack of suitably stable plasmids and the need for a fluorescent tag that can be used in tandem with green fluorescent protein (GFP). Vectors previously used in V. fischeri contain the p15A replication origin; however, we found that this replicon is not stable during growth in the host and is retained by fewer than 20% of symbionts within a day after infection. In contrast, derivatives of V. fischeri plasmid pES213 were retained by approximately 99% of symbionts even 3 days after infection. We therefore constructed pES213-derived shuttle vectors with a variety of selectable and visual markers. To include a visual tag that can be used in conjunction with GFP, we compared seven variants of the DsRed2 red fluorescent protein (RFP): mRFP1, tdimer2(12), DsRed.T3, DsRed.T4, DsRed.M1, DsRed.T3_S4T, and DsRed.T3(DNT). The last variant was brightest, displaying >20-fold more fluorescence than DsRed2 in V. fischeri. RFP expression did not detectably affect the fitness of V. fischeri, and cells were readily visualized in combination with GFP-expressing cells in mixed infections. Interestingly, even when inocula were dense enough that most E. scolopes hatchlings were infected by two strains, there was little mixing of the strains in the light organ crypts. We also used constitutive RFP in combination with the luxICDABEG promoter driving expression of GFP to visualize the spatial and temporal induction of this bioluminescence operon during symbiotic infection. Our results demonstrate the utility of pES213-based vectors and RFP for in situ experimental approaches in studies of the V. fischeri-E. scolopes symbiosis.  (+info)

Cyclo(Phe-Pro) modulates the expression of ompU in Vibrio spp. (8/147)

Vibrio vulnificus was found to produce a chemical that induced the expression of Vibrio fischeri lux genes. Electron spray ionization-mass spectrometry and 1H nuclear magnetic resonance analyses indicated that the compound was cyclo(L-Phe-L-Pro) (cFP). The compound was produced at a maximal level when cell cultures reached the onset of stationary phase. Sodium dodecyl sulfate-polyacrylamide gel analysis of the total proteins of V. vulnificus indicated that expression of OmpU was enhanced by exogenously added synthetic or purified cFP. A toxR-null mutant failed to express ompU despite the addition of cFP. The related Vibrio spp. V. cholerae, V. parahaemolyticus, and V. harveyi also produced cFP, which induced the expression of their own ompU genes. cFP also enhanced the expression in V. cholerae of the ctx genes, which are known to be regulated by ToxR. Our results suggest that cFP is a signal molecule controlling the expression of genes important for the pathogenicity of Vibrio spp.  (+info)

*Bobtail squid

... have a symbiotic relationship with bioluminescent bacteria (Aliivibrio fischeri), which inhabit a special light ... Symbiosis with A. fischeri from the surrounding seawater is initiated immediately upon hatching, and the bacteria's ...

*Bioluminescent bacteria

For example, Aliivibrio fischeri grows favourably in environments that have temperatures between 5 to 30°C and a pH that is ... Unlike Aliivibrio fischeri, V. harveyi do not possess the luxI/luxR regulatory genes and therefore have a different mechanism ... A well known example of quorum sensing is that which occurs between Aliivibrio fischeri and its host. This process is regulated ... In bioluminescent bacteria, the reclassification of the members ofVibrio fischeri species group as a new genus, Aliivibrio, has ...

*Aliivibrio fischeri

Vibrio salmonicida and Vibrio wodanis as Aliivibrio fischeri gen. nov., comb. nov., Aliivibrio logei comb. nov., Aliivibrio ... Aliivibrio fischeri is a Gram-negative, rod-shaped bacterium found globally in marine environments. A. fischeri has ... In 2014, Hawaiʻian State Senator Glenn Wakai submitted SB3124 proposing Aliivibrio fischeri as the state microbe of Hawaiʻi. ... The genome for A. fischeri also carries mobile genetic elements. Planktonic A. fischeri bacteria are found in very low ...

*Vibrio azasii

It iss somehow related to Aliivibrio fischeri.[citation needed] It is native to the Red Sea, and lives in symbiosis with big- ...

*State microbes

Aliivibrio fischeri was selected because it lives in a symbiotic relationship with the native Hawaiʻian bobtail squid, in which ... In February 2014, Senate Bill 3124 was introduced by Senator Glenn Wakai; the bill designates Aliivibrio fischeri as the State ... Aliivibrio fischeri can be found elsewhere. The combined Hawaiʻian Legislature could not agree on which microbe better suited ... and later contested in 2014 by Senator Glenn Wakai's SB3124 bill proposing Aliivibrio fischeri instead. As of December 2017[ ...

*Euprymna scolopes

"Aliivibrio fischeri lipopolysaccharide induces developmental apoptosis, but not complete morphogenesis, of the Euprymna ... "Symbiotic role of the viable but nonculturable state of Aliivibrio fischeri in Hawaiian coastal seawater" (PDF). Archived from ... "Alterations in the protoeme of the Euprymna scolopes light organ in response to symbiotic Aliivibrio fischeri" (PDF). Archived ... "Isolation and characterization of a visibly luminous variant of Aliivibrio fischeri strain ES114 form the sepiolid squid ...

*Counter-illumination

The light may be produced by the animals themselves, or by symbiotic bacteria, often Aliivibrio fischeri. Counter-illumination ... The luminescent bacterium is often Aliivibrio fischeri, as for example in the Hawaiian bobtail squid, Euprymna scolopes. ...

*Vibrionaceae

Vibrio salmonicida and Vibrio wodanis as Aliivibrio fischeri gen. nov., comb. nov., Aliivibrio logei comb. nov., Aliivibrio ... and Aliivibrio wodanis comb. nov". International Journal of Systematic and Evolutionary Microbiology. 57 (12): 2823-2829. doi: ... The family Vibrionaceae currently comprises eight validly published genera: Aliivibrio, Catenococcus, Enterovibrio, Grimontia, ...

*Glenn Wakai

In 2014, Wakai proposed SB3124 which attempted to establish Aliivibrio fischeri as the state microbe of Hawaiʻi. This was ...

*Quorum sensing

... was first observed in Aliivibrio fischeri, a bioluminescent bacterium that lives as a mutualistic symbiont in ... The YenR and YenI proteins produced by the gammaproteobacterium Yersinia enterocolitica are similar to Aliivibrio fischeri LuxR ... When A. fischeri cells are free-living (or planktonic), the autoinducer is at low concentration, and, thus, cells do not ... paradigm as defined by the Vibrio fischeri system occur in the Gram-negative Proteobacteria. A comparison between the ...

*Flavobacterium akiainvivens

... was deferred for a year when it encountered competition from Senator Glenn Wakai's SB3124 proposing Aliivibrio fischeri. In ...

*Bioluminescence

They used bacteria called Aliivibrio fischeri which glow in the dark, but the maximum lifetime of their product was three days ... "The first engagement of partners in the Euprymna scolopes-Vibrio fischeri symbiosis is a two-step process initiated by a few ...

*James Tokioka

... was deferred for a year when it encountered competition from Senator Glenn Wakai's SB3124 proposing Aliivibrio fischeri. 2012 ...

*Isaac Choy

... and later contested in 2014 by Senator Glenn Wakai's SB3124 bill proposing Aliivibrio fischeri instead. As of December 2017[ ...

*Aliivibrio

Vibrio salmonicida and Vibrio wodanis as Aliivibrio fischeri gen. nov., comb. nov., Aliivibrio logei comb. nov., Aliivibrio ... Aliivibrio is a genus in the phylum Proteobacteria (Bacteria). The name Aliivibrio derives from: Latin alius, other, another, ... Aliivibrio entry in LPSN [Euzéby, J.P. (1997). "List of Bacterial Names with Standing in Nomenclature: a folder available on ... and Aliivibrio wodanis comb. nov". International Journal of Systematic and Evolutionary Microbiology. 57 (12): 2823-2829. doi: ...

*Spot 42 RNA

Aliivibrio fischeri and Aliivibrio salmonicida the spf gene is flanked by polA (upstream) and a sRNA gene encoding the novel ... "Expression profiling reveals Spot 42 small RNA as a key regulator in the central metabolism of Aliivibrio salmonicida". BMC ... Aliivibrio, Photobacterium and Grimontia genera). In e.g., Vibrio cholerae, Vibrio vulnificus, ... and the majority of experimental work on Spot42 has been performed in Escherichia coli and recently in Aliivibrio salmonicida. ...

*List of model organisms

Mycoplasma genitalium - a minimal organism Aliivibrio fischeri - quorum sensing, bioluminescence and animal-bacterial symbiosis ...

*Vibrio harveyi

Quorum sensing was first studied in V. fischeri (now Aliivibrio fischeri), a marine bacterium that uses a synthase (LuxI) to ... Despite its relatedness to A. fischeri, V. harveyi lacks a LuxI/R quorum-sensing system, and instead employs a hybrid quorum- ...
Coloured scanning electron micrograph (SEM) of Vibrio fischeri, Gram-negative, motile, bioluminescent, curved rod-shaped bacterium (prokaryote). Vibrio fischeri is a bioluminescent bacterium that occurs in global ocean environments. V. fischeri is most often found in a symbiotic relationship with certain deep sea marine life, such as the bobtail squid, within special light-organs.. V. fischeri causes the squid to produce a mucus substance that is secreted below the light organ on the squid. Cilia are used to draw more bacteria into this mucus matrix. The bacteria migrate into the light organ and colonize an area within it. They provide a light source for the squid to make it blend in with the ocean floor at night. Thus predators have a hard time seeing the squid. V. fischeri is a key research organism for examination of microbial bioluminescence, quorum sensing, and bacterial-animal symbiosis. Magnification: x8,000 when shortest axis printed at 25 - Stock Image C032/1744
Courtesy of: http://dels-old.nas.edu/oceans/oceans_and_human_health_part_2.shtml Introduction: This blog examines the relationship between the Hawaiian Bobtail Squid and a bioluminescent bacterium. The Hawaiian Bobtail squid acquires an enhanced ability to capture prey and avoid predators, while the bacterium receives key nutrients from residing within the squid [2]. Distribution of this aquatic relationship is limited by the habitat of the squid (Hawaii). Several bioluminescent bacteria cycle through a single squid daily. Description of Relationship: The type of relationship observed between Euprymna scolope(Hawaiian Bobtail squid) and Vibrio fischeri(bioluminescent bacterium) is unique to organisms with specialized structures called light organs. These light organs are specialized structures that have evolved to allow the organisms that house them to create light. While the evolutionary reason for this structure is unknown, many individuals hypothesize that certain marine organisms form these ...
Description: In the Hawaiian bobtail squid (pictured), scientists have found that the same bacterial toxin that causes whooping cough and gonorrhea in humans is responsible for the development of an important organ. The finding, reported in the Nov. 12 Science magazine, suggests that the role of bacteria in their host organisms depends significantly on the biological context in which they are found. In one place, a bacterium may have a harmful role; in another, it may be critical for the animals survival. The corresponding author of the Science paper is Margaret McFall-Ngai, a UW-Madison professor of medical microbiology ...
Bobtail squid (order Sepiolida) are a group of cephalopods closely related to cuttlefish. Bobtail squid tend to have a rounder mantle than cuttlefish and have no cuttlebone. They have eight suckered arms and two tentacles and are generally quite small (typical male mantle length being between 1 and 8 cm). Sepiolids live in shallow coastal waters of the Pacific Ocean and some parts of the Indian Ocean and Atlantic Ocean as well as in shallow waters on the west coast of the Cape Peninsula off South Africa. Like cuttlefish, they can swim by either using the fins on their mantle or by jet propulsion. They are also known as dumpling squid (owing to their rounded mantle) or stubby squid. Bobtail squid have a symbiotic relationship with bioluminescent bacteria (Aliivibrio fischeri), which inhabit a special light organ in the squids mantle. The bacteria are fed a sugar and amino acid solution by the squid and in return hide the squids silhouette when viewed from below by matching the amount of light ...
Peptidoglycan recognition proteins (PGRPs) are mediators of innate immunity and recently have been implicated in developmental regulation. To explore the interplay between these two roles, we characterized a PGRP in the host squid Euprymna scolopes (EsPGRP1) during colonization by the mutualistic bacterium Vibrio fischeri. Previous research on the squid-vibrio symbiosis had shown that, upon colonization of deep epithelium-lined crypts of the host light organ, symbiont-derived peptidoglycan monomers induce apoptosis-mediated regression of remote epithelial fields involved in the inoculation process. In this study, immunofluorescence microscopy revealed that EsPGRP1 localizes to the nuclei of epithelial cells, and symbiont colonization induces the loss of EsPGRP1 from apoptotic nuclei. The loss of nuclear EsPGRP1 occurred prior to DNA cleavage and breakdown of the nuclear membrane, but followed chromatin condensation, suggesting that it occurs during late-stage apoptosis. Experiments with purified
Bioluminescent bacteria are light-producing bacteria that are predominantly present in sea water, marine sediments, the surface of decomposing fish and in the gut of marine animals. While not as common, bacterial bioluminescence is also found in terrestrial and freshwater bacteria. These bacteria may be free living (such as Vibrio harveyi) or in symbiosis with animals such as the Hawaiian Bobtail squid (Aliivibrio fischeri) or terrestrial nematodes (Photorhabdus luminescens). The host organisms provide these bacteria a safe home and sufficient nutrition. In exchange, the hosts use the light produced by the bacteria for camouflage, prey and/or mate attraction. Bioluminescent bacteria have evolved symbiotic relationships with other organisms in which both participants benefit close to equally. Another possible reason bacteria use luminescence reaction is for quorum sensing, an ability to regulate gene expression in response to bacterial cell density. Records of bioluminescent bacteria existed for ...
The developmental changes bobtail squid undergo when colonized by the bacteria are both dramatic and accessible for biologists to study. The bobtail squid, Euprymna scolopes, hatches as a fully formed squid. They have no larval stage. However, theres a vestige of the ciliated structures seen in other mollusks. (Cilia are like tiny hairs on the surface of individual cells.) These ciliated cells cover the surface of a pair of "appendages" in the undeveloped light organ (Koropatnick, 2014). After hatching, the bobtail squid has no association with any bacteria, and is not capable of luminescence. The light organ can only complete its development once it is colonized by a specific species of bacteria, Vibrio fischeri. Once V. fischeri is obtained from the surrounding seawater, the bacteria become suspended in mucus and migrate to the light organ. The cells smell their way! They follow a trail of chemical cues left by the squids cells (Koropatnick, 2014). Approximately 12 hours after hatching, the ...
The Genomic tRNA Database is curated by Todd Lowe and Patricia Chan. Please email [email protected] with any questions and comments. To submit corrections, please use our Bug and Request Tracking System ...
Aliivibrio fischeri ATCC ® 700601D-5™ Designation: Genomic DNA from Aliivibrio fischeri strain ES114 TypeStrain=False Application:
... - The other day I decided I wanted to make some sort of sea creature, & after a bit of research I decided the Bobtail Squid was the one for me. I wr
The relationship between the Hawaiian bobtail squid and the bacterium Vibrio fischeri is well chronicled, but writing in the current issue of the journal Proceedings of the Royal Society B, a group led by University of Wisconsin-Madison microbiologists Margaret McFall-Ngai, Edward Ruby and their colleagues adds a new wrinkle to the story.
Homologs of Toxin-Encoding Genes. Although V. fischeri is not known to be pathogenic, and strain ES114 is a beneficial symbiont, its genome carries homologs of Vibrio genes that may have toxin activity (Table 3, which is published as supporting information on the PNAS web site). As mentioned earlier, these include two CTX phage-encoded genes, zot (zona occludins toxin) and ace (accessory cholera enterotoxin), the latter of which has been found only in the V. cholerae and V. fischeri genome sequences. The proteins coded for by these genes have been shown to contribute to the structure of CTX phage (17), and their possible roles as toxins remain controversial (31, 32). At this time, it is not known whether the V. fischeri homologs of these two genes are expressed in this species, or whether they might play a role in this bacteriums symbiotic associations.. All sequenced Vibrio species carry genes encoding another putative toxin called RTX (repeats in structural toxins). RTX activity in V. ...
The American Bobtail, a relative newcomer on the scene, is increasing in popularity due to its winning combination of wild appearance and friendly demeanour.. Among the few cat breeds that have originated in the United States, the American Bobtail did not receive recognition by the Cat Fanciers Association until February 2000.. American Bobtail History. The first known American Bobtail was Yodie, a stray with a stubby tail who was found by vacationers in the 1960s. Although his parentage is unknown, it was rumoured that he was a hybrid of a bobcat and a domestic cat due to his short tail and wild look. However, this is unlikely, as the males derived from such matings tend to be sterile, whereas Yodie was not. It is more likely that Yodies stumpy tail was the result of a spontaneous mutation.. Yodie was mated at first with a seal point Siamese female and the kittens were born with bobbed tails, which indicated that the bobtail gene was dominant. Yodie and his children were subsequently mated ...
We demonstrate an approach to the surface-mediated release of a synthetic N-acylated l-homoserine lactone (AHL) modulator of bacterial quorum sensing (QS). AHL released gradually from thin films of poly(lactide-co-glycolide) (PLG) is shown to activate QS in the model symbiont Vibrio fischeri at levels that e
Glowing bacteria inside squids use light and chemical signals to control circadian-like rhythms in the animals, according to a study to be published in mBio®. The Hawaiian bobtail squid, Euprymna ...
A previously applied alanine-scanning mutagenesis that was performed with the E. coli csrA gene, used to examine the relative contribution each amino acid residue to CsrA protein function [20], provides an invaluable comparator to our spontaneous suppression screen. Our complementary screen differs in that all potential amino acid substitutions are possible, even the single native alanine residue that was not altered in the alanine scan. In our screen, an A36S substitution caused a 20-fold increase in luminescence and restored siderophore activity indicating the highly conserved A36, positioned into the hydrophobic core of the CsrA homodimer, could be critical to protein folding and 3- dimensional stability [20, 23]. Even the conservative substitution of this alanine with the non-polar and fairly compact valine (VCW2G2) altered CsrA function (Fig. 2). Also, only mutations that improve growth would likely improve fitness. Thus, mutations that map to non-conserved regions that do not contribute to ...
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Vision in fishes and crustaceans is a fascinating and understudied area. In past decades, there were far more studies on the visual systems of sea-dwelling creatures, but with the push towards applied or translational research, the number of reports in these species have dropped off, much to our detriment as one never knows where the […]. ...
Until recently the American Bobtail has received little attention, so most people are surprised to learn that it has been catting around America for as long as the better-known Japanese Bobtail (see p
This activity explores the content presented in the animated video Natures Cutest Symbiosis: The Bobtail Squid, which describes how the light produced by bacteria living inside bobtail squid helps the squid avoid its predators.. ...
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on my 3 element 40 meter bobtail fed at the top of the center element, it made a huge difference how it was wired... I had to connect the center conductor of the coax to the vertical elemennt and the shield to the horizontal phasing wire. It allowed me to work(and conform) my last 3 zones on 40 ...
Description. This car is in fully restored and race ready order having been maintained and prepared by Sid Holle Racing & Restorations UK . Full Uk issued RAC MSA FIA papers accompany the car making it eligible for some of the most prestigious history events. Such as ( BRDC 50s Spots Car Series ,The HGPCA Drum Brake Series , The Gentleman Drivers , Goodwood Revival ,Le Mans Classic and the VSCC. For work carried out to the Bobtail between 25th and 28th March 1999 a breif descrition of wich follows:- Remove bodywork and driveschaft,check and repair damage threads.Refit schaft& checkgear selection.Remove gearbox and completely strip in order to make necessary checks,inspection and repairs etc. Supplied and fitted new 3rd Gear. Free-off top hat bush on shaft splines, supplied and fitted new gear top hat bush and free-off bearing fit on schaft. Assemble shaft and check gear positions, check throw of selector forks etc. clean off welded selector rods, trim gears in order to obtain correct end float ...
Model of integrated biofilm and QS regulatory networks in Vibrio fischeri. Environmental cues, including conditions associated with the host during initial colo
Members of the class Cephalopoda are gonochoric. Male and female adults usually die shortly after spawning and brooding, respectively. Mating behavior: Males perform various displays to attract potential females for copulation. During copulation, male grasp the female and inserts the hectocotylus into the females mantle cavity where fertilization usually occurs. Life cycle: Embryos hatch into planktonic stage and live for some time before they grow larger and take up a benthic existence as adults (Ref. 833). ...
Squid typically arent my thing, but I can certainly be wooed by their microbes. Example: the very awesome symbiotic relationship between the Hawaiian bobtail
Many recent studies rely on 16S rRNA-based sequencing approaches to analyze bacterial or archaeal communities found in soil and other environmental samples. While this approach is valuable for determining the relative abundances of different microbial taxa found in a given sample, it does not provide information on how the total abundances of targeted microbes differ across samples. Here we demonstrate how the simple addition of an internal standard at the DNA extraction step allows for the quantitative comparison of taxon abundances across samples. The reliability of this method was assessed in two ways. First, we spiked a dilution series of two different soils with internal standards to ascertain whether we could accurately quantify differences in cell abundances. We tested two different internal standards, adding DNA from Aliivibrio fischeri or Thermus thermophilus, bacterial taxa unlikely to be found in soil. Both standards allowed us to accurately quantify microbial abundances in soil as ...
Presence of a naturally shortened tail. Description: DNA test can identifying dogs carrying the phenotype "Natural Bobtail". Symptoms : Shortened tail, variability according to individuals: missing tail to 3/4 of tail length. Age of onset : _. Frequency : _. Gene involved : T. Tested mutation : c.295C>G. Transmission : Autosomal Dominant. Patent : _. Notes : _. PrintSend to a friendTweet WidgetPartager sur Facebook ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Component of the acetyl coenzyme A carboxylase (ACC) complex. Biotin carboxylase (BC) catalyzes the carboxylation of biotin on its carrier protein (BCCP) and then the CO(2) group is transferred by the transcarboxylase to acetyl-CoA to form malonyl-CoA.
Looking for online definition of RSCS in the Medical Dictionary? RSCS explanation free. What is RSCS? Meaning of RSCS medical term. What does RSCS mean?
Eventbrite - MadLab presents Culturing bioluminescent bacteria - Sunday, November 4, 2012 at MadLab , Manchester, Gt Man. Find event and ticket information.
Why would someone want to add a fragment of dead coral to a liquid culture of bioluminescent bacteria. I thought why not. This is what happened.
The Bobtail Lizard is often seen sun basking on the road - please try not to run them over as they bond with their mate for life and raise their young together.
Many bacteria, including V. fischeri, recognize and respond to their environments using two-component regulatory systems (Fig. 2A, reviewed in Stock et al.
Effect of CIAP treatment of lipid A on its ability to induce juvenile light organ apoptosis. (A) Fluorescence micrographs of light organs that had been incubate
Turraea fischeri is an East African traditional herb, which is widely used in traditional medicine. In this study, we profiled the secondary metabolites in the methanol extract of T. fischeri bark using HPLC-PDA-ESI-MS/MS, and 20 compounds were tentatively identified. Several isomers of the flavonolignan cinchonain-I and bis-dihydroxyphenylpropanoid-substituted catechin hexosides dominated the extract. Robust in vitro and in vivo antioxidant properties were observed in 1,1-diphenyl-2-picrylhydrazyl radical scavenging assay (DPPH) and ferric reducing antioxidant power (FRAP) assay, and in the model organism Caenorhabditis elegans. Additionally, the extract exhibited promising hepatoprotective activities in D-galactosamine (D-GaIN) treated rats. A significant reduction in the elevated levels of aspartate aminotransferase (AST), total bilirubin, gamma-glutamyltransferase (GGT), and malondialdehyde (MDA) and increase of glutathione (GSH) was observed in rats treated with the bark extract in addition to D
In recent years it has become clear that the production of N-acyl homoserine lactones (N-AHLs) is widespread in Gram-negative bacteria. These molecules act as diffusible chemical communication signals (bacterial pheromones) which regulate diverse physiological processes including bioluminescence, antibiotic production, piasmid conjugal transfer and synthesis of exoenzyme virulence factors in plant and animal pathogens. The paradigm for N-AHL production is in the bioluminescence (lux) phenotype of Photobacterium fischeri (formerly classified as Vibrio fischeri) where the signalling molecule N-(3-oxohexanoyl)-L-homoserine lactone (OHHL) is synthesized by the action of the Luxl protein. OHHL is thought to bind to the LuxR protein, allowing it to act as a positive transcriptional activator in an autoinduction process that physiologically couples cell density (and growth phase) to the expression of the bioluminescence genes. Based on the growing information on Luxl and LuxR homologues in other ...
Find bars, restaurants and things to do near 832 W. Superior St., the Chicago location of Living Light: Photographs of Bioluminescent Bacteria on Metromix Chicago.
A Novel GH7 Endo-β-1,4-Glucanase from Neosartorya fischeri P1 with Good Thermostability, Broad Substrate Specificity and Potential Application in the Brewing Industry. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Vibrio fischeri thiL, pgpA, luxP, luxQ, dxs, ispA genes for thiamine monophosphate kinase, phosphatidylglycerophosphatase A, bioluminescence regulatory protein, bioluminescence regulatory protein, 1-deoxyxylulose-5-phosphate synthase, geranyltranstransferase, complete and partial ...
A feedback-controlled oxystat system is described maintaining steady-state O2 partial pressures (pO2) between 0.01 mmHg (14 nM-O2) and 150 mmHg (210 microM-O2) and simultaneously monitoring O2 uptake at rates between 0.1 and 120 microM-O2 X min-1 in suspensions of cells, in subcellular fractions and in solutions of enzymes. At pO2 values between 0.2 and 150 mmHg (0.28 and 210 microM-O2) a polarographic O2 sensor was used, and below a pO2 of 0.2 mmHg (0.28 microM-O2) the O2-dependent luminescence of the photobacterium Vibrio fischeri was utilized to monitor the actual pO2. At a selected pO2, O2 supply is maintained by injecting appropriate amounts of O2-saturated aqueous medium into the reaction chamber by using a motor-driven burette. The oxystat system is under control of a computer that reads the O2 sensors, interacts with the motor-driven burette, calculates the O2 uptake from the amounts of O2-saturated medium added, collects data from further measuring devices and provides the documentation ...
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...In order to pass on their genes southern dumpling squid engage in up ...The research provides new insight into the evolution of reproductive s...The research was conducted by Master of Science student Ms Amanda Fran...The team studied dumpling squid ( Euprymna tasmanica ) that live ...,Promiscuous,squid,fatigued,after,mating,biological,biology news articles,biology news today,latest biology news,current biology news,biology newsletters
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This new limited edition "Headphonies" Hello Kitty collectible comes in the shape of a portable micro speaker, designed like the popular Sanrio bobtail cat with a pair of red headphones on her head.. The wireless and travel-friendly speaker features a built-in rechargeable lithium-ion battery. To charge it, simply connect Kitty to a USB port. One hour of charging time will give you about six hours of play time.. ...
Despite the increasing use of rare earth elements (REEs) and oxides (REOs) in various technologies, the information on their ecotoxicological hazard is scarce. Here, the effects of La3+, Ce3+, Pr3+, Nd3+, Gd3+, CeO2, and eight doped REOs to marine bacteria Vibrio fischeri and freshwater protozoa Tetrahymena thermophila were studied in parallel with REO dopant metals (Co2+, Fe3+, Mn2+, Ni2+, Sr2+). The highest concentrations of REOs tested were 100 mg/L with protozoa in deionized water and 500 mg/L with bacteria in 2% NaCl. Although (i) most REOs produced reactive oxygen species; (ii) all studied soluble REEs were toxic to bacteria (half-effective concentration, EC50 3.5-21 mg metal/L; minimal bactericidal concentration, MBC 6.3-63 mg/L) and to protozoa (EC50 28-42 mg/L); and (iii) also some dopant metals (Ni2+, Fe3+) proved toxic (EC50 ≤ 3 mg/L), no toxicity of REOs to protozoa (EC50 > 100 mg/L) and bacteria (EC50 > 500 mg/L; MBC > 500 mg/L) was observed except for La2NiO4 (MBC 25 mg/L).
http://en.wikipedia.org/wiki/Aliivibrio_fischeri V. fischeri] is a classic example of gram negative bacterial quorum sensing,cite,#Fuqua,/cite,. The system uses two regulator proteins that have analogies in other QS systems, LuxI and LuxR. LuxI is a protein that synthesizes the signal, [http://en.wikipedia.org/wiki/Autoinducer#Vibrio_fischeri:_bioluminescence N-(3-oxohexanoyl)-homoserinelactone] (HSL), called the autoinducer. The autoinducer then diffuses out of the original sender and into neighboring cells. If V. fischeri density is low, there is a low HSL concentration. Similarly, at high densities, there is high HSL concentration. At a particular threshold concentration, HSL will bind to and activate LuxR, a protein which activates transcription of a set of genes that include bioluminescence and luxI. luxI transcription leads to further production of LuxI, creating a positive feedback loop that creates rapid light generation. However, since LuxR negatively regulates luxR, ...
An alternative approach was planned during the early stages of the project: the combination of LuxAB with another protein: YFP from Vibrio fischeri. This would shift the wavelength produced by LuxAB towards the green regions of the spectrum, to activate our green light sensor. During our discussions with UNAM-Genomics Mexico we realised that they planned on making the same part, so we stopped working on it, planning on testing its combination with our green light sensor later on. We unfortunately have not yet reached that stage, but the part is available in the registry as BBa_K360010.. In addition to the above, we created a codon-optimised mutant of the firefly luciferase, reported by Fujii et al. (2007) to be 12.5 times brighter than the wildtype. This may help to alleviate the problems foreseen with a lack of luminescence intensity failing to activate the light sensors of the corresponding wavelength.. LUCIE (LUCiferase with Intense Emission) has three mutations compared to the wild type: ...
Targeted gene and genome manipulation via homologous recombination in bacteria relies on the use of marker genes. In marker-less gene replacement, a negative and a positive selection marker are employed, in a process that normally involves two transformation steps with two DNA suicide vectors. In this work, we developed an alternative strategy for marker-less gene replacement in Synechocystis, based on the use of a single plasmid and a single transformation step. The technique was tested by inserting the luxAB operon from Vibrio fischeri into the neutral receptor site slr0168. To this end the wild-type strain was transformed with the vector pDSlux and the transformation efficiency with this integration vector was in the range of 10-5, while the average frequency of the second recombination event was 10-6 (see Table 1). This ten-fold difference could be due to the fact that the first recombination event involves a double crossover between the genomic DNA and the plasmid vector, whereas the second ...
Roberto is a 19 years old, undergraduate student in Biology and Microbiology at the Andes University. He is fascinated by the regulation systems implied in cellular biology, especially with epigenetic processes and the post-transcriptional regulation systems as the RNA Induced Silencing Complex. Now that he is involved with Synthetic Biology he thinks it is one of the most powerful tools of these era, and he dream to work some day on a synthetic biology mediated response against pathogenic infections as HIV and Malaria. In his experience trough IGEM he is focused on stracting the genes we need out of Vibrio fischeri to create the new biobricks for our project, he also may be a little bit distracted but the fines he payed for that and his work are really helpful. He got involved with Molecular Biology in the 2002, at the Molecular Biology Estudy Center of the School Gimnacio Campestre in Bogotá [1]. There he worked on different projects including: The development of the Gorgona`s turtle ...
The plenary session was opened by the coordinator of ATHON - Dr. Helen Håkansson of the Karolinska Institutet in Sweden - who gave a brief introduction on the ATHON project and the relevance of mixture toxicity for NDL-PCB risk assessment. Next, Dr. Thomas Backhaus (University of Gothenburg, Sweden), gave an introduction to the general principles of "concentration addition" (CA) and "independent action" (IA) within mixture toxicity, which was illustrated with an abundance of practical examples. The general conclusion from his presentation was that for hazard and risk assessment, the selection between a CA or IA model seems to be of minor importance. Keeping in mind the adage "seek simplicity but distrust it", Dr. Backhaus advocated that CA might be a simple default assumption to start with and to work further from. The third plenary speaker was Dr. Niklas Johansson (Swedish EPA and Karolinska Institutet) who demonstrated the use of a Central Composite, Face centered (CCF) statistical test ...
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Join Peter Rabbit in the beautiful Lake District along with his friends Benjamin Bunny and Lily Bobtail as they encounter real dangers, overcome obstacles and outsmart predators all while finding fun and excitement in the most unexpected places.. ...
Welcoming in the New Year should be a time of joyous celebration. Unfortunately, it is also a common time for injuries. For example, on New Years Eve this
Operating at up to 75ppm (LTR), the imageRUNNER 5075 delivers advanced document handling and finishing capabilities to mid-to-high volume office and light production environments.
I am a mystic. You will see that most of my travel takes me to places of power where I access the lessons of nature.. Hawaii: Oahu, Hawaii (Big Island), Maui, ...
HPLC Application #21624: FMOC-Pro-OH on Lux 5µm Amylose-2 in RP. Column used: Lux® 5 µm Amylose-2, LC Column 250 x 4.6 mm, Ea Part#: 00G-4472-E0
Characterization of BBa_F2620, an engineered cell-cell communication device Anna Labno, Barry Canton, Drew Endy Division of Biological Engineering, MIT Current research in synthetic biology aims to enable the design and construction of living organisms in the manner that we now engineer electrical, mechanical, and other systems. To facilitate design of higher-order systems, we need to be able to predict the behavior of a system from the characteristics of the components that might comprise it. Thus, designing and using a methodology for characterizing the performance of biological parts and devices is central to the future success of biological engineering. Here, we engineered a cell-cell communication device, BBa_F2620, based on parts from the quorum sensing system of Vibrio fischeri [1]. BBa_F2620 is a receiver device that responds to the concentration of a small signaling molecule (AHL) in the media by modulating the transcription rate that is output from the device. To begin to ...
Shifts in the membrane composition of two strains of Pseudoronas putida(solvent sensitive strain MW! 200 and solvent tolerant strain Idaho) were studied following exposure to solvent (o-xylene). Responses to solvent were examined in terms of phospholipid and fatty acid content (increasing in strain Idaho, and decreasing in strain MW1200), and synthesis and composition of lipopolysaccharide (again increasing in strain Idaho and decreasing in strain MW1200). Possibly due to the alteration in LPS structure, stable biofilms of strain Idaho formed more quickly in the presence of solvent than was observed in the absence of solvent. Also, changes in the biosynthesis rate of the phospholipids in the two strains were measured. Both strains exhibited an increase in biosynthesis rate in the presence of o-xylene with the greatest increase in strain Idaho. The increased rate of biosynthesis is highly beneficial to the Idaho cells enabling rapid repair of membrane damage and, therefore an increased solvent tolerance
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Anna Maria Cicero is the author of these articles in the Journal of Visualized Experiments: Long-term Lethal Toxicity Test with the Crustacean Artemia franciscana, Ecotoxicological Method with Marine Bacteria Vibrio anguillarum to Evaluate the Acute Toxicity of Environmental Contaminants
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I started breeding Siamese in 1973. I started with chocolate and seal point Siamese. The name "Raksha" comes from a Roger Zelazney novel featuring a modified Hindu pantheon/mythology. In this book a Raksha was a small mischeivious shape changing demon .....sound familiar? Yes, I thought so too. Originally registered with CFA and ACFA , I quickly switched to TICA when it became a reality and have not looked at the other associations since. Over the years I have bred red points and tortie points. I branched out into Oriental Shorthairs thanks to my good friend , Lindajean Grillo. I finally moved onto Balinese and Oriental Longhairs as well. I like all show people have been tempted by other breeds and have had Abyssinians, Himalayans. Birmans, American Curls,Japanese Bobtails and a Maine Coon. While these are all beautiful cats, I have learned that they dont have the personality that attacts me to Siamese. Although I will say that my Birman JanipursWWW.Clarice.com was such a darling that I and ...
Article Metal mixture modeling evaluation project: 3. Lessons learned and steps forward. A comparison of 4 metal‐mixture toxicity models (that were based on the Biotic Ligand Model and WHAM‐FTOX) was presented in the previous paper. In the present st...
Department of Agriculture, Animal Quarantine Facility Oahu Community Correctional Center Halawa Correctional Facility Mililani Technology Park, Lot...
North Shore beaches of Oahu are both beautiful and distinctively unique. Use this guide to find the perfect beach for your planned activity.
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Some USGS information accessed through this means may be preliminary in nature and presented without the approval of the Director of the USGS. This information is provided with the understanding that it is not guaranteed to be correct or complete and conclusions drawn from such information are the responsibility of the user ...
Some USGS information accessed through this means may be preliminary in nature and presented without the approval of the Director of the USGS. This information is provided with the understanding that it is not guaranteed to be correct or complete and conclusions drawn from such information are the responsibility of the user ...
I got rid of the sensation of a lump in my throat with a couple of easy life style changes from my medical professional! And although modern day medicine has resulted in some deaths that would not have happened otherwise (see Vioxx), it has also saved vastly far more lives than would have been saved
The marine bacterium Vibrio harveyi controls its bioluminescence by a process known as quorum sensing. In this process, autoinducer molecules are detected by membrane-bound sensor kinase/response regulator proteins (LuxN and LuxQ) that relay a signal via a series of protein phosphorylation reactions to another response regulator protein, LuxO. Phosphorylated LuxO indirectly represses the expression of the proteins responsible for bioluminescence. Integral to this quorum sensing process is the function of the phosphotransferase protein, LuxU.
Connexin 40 (Cx40) is expressed by the renin-producing cells (RSCs) of the kidneys and the endothelial cells of blood vessels. Cx40 null mice (Cx40−/−) feature a much increased renin synthesis and secretion, which results in chronic hypertension, and also display an altered endothelium-dependent relaxation of the aorta because of reduced eNOS levels and nitric oxide production. To discriminate the effect of Cx40 in renin secretion and vascular signaling, we targeted Cx40 to either the RSCs or the endothelial cells of Cx40 null mice. When compared with Cx40−/− controls, the animals expressing Cx40 in RSCs were less hypertensive and featured reduced renin levels, still numerous RSCs outside the wall of the afferent arterioles. In contrast, mice expressing Cx40 in the endothelial cells were as hypertensive as Cx40−/− mice, in spite of control levels of Cx37 and eNOS. Our data show that blood pressure is improved by restoration of Cx40 expression in RSCs but not in endothelial cells, ...
I find the chart confusing and contradictory. It says that random-bred cats are shown in italics, and then has American shorthair in roman font. Im fairly certain that "American shorthair" is basically a bucket for any short-haired cat found in America that is not pure-bred. At least, "domestic shorthair" is what my vet has put on the medical record of each of my cats (provenance: trailer park, redneck family who dont get their animals neutered, barn, and stray, respectively). Likewise, I read elsewhere that the paper showed that the Japanese bobtail was more closely related to European than Asian lineages, but the chart shows J. Bobtail right there in the same branch with the other Asian cats. Many years ago, I read that it was believed that "temple cats" were domesticated independently of other cats. That would be the applehead* Siamese (not shown!), Siamese, Burmese, etc. The abstract would seem to imply that this is not the case, but again, the chart shows all the temple breeds clustered ...
2383248] The nucleotide sequence of the luxA and luxB genes of Xenorhabdus luminescens HM and a comparison of the amino acid sequences of luciferases from four species of bioluminescent bacteria. (Biochem Biophys Res Commun. , 1990 ...
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Article The effect of binary mixtures of Zn, Cu, Cd, and Ni on the growth of the freshwater diatom Navicula pelliculosa and comparison with mixture toxicity model predictions. We investigated the effect of binary mixtures of Zn, Cu, Cd, and Ni on the...
Ondracek K, Bandouchova H, Hilscherova K, Kovacova V, Linhart P, Miksikova M, Mlcakova V, Osickova J, Pohanka M, Skochova H, Pikula J. Mixture toxicity of microcystin-LR, paraoxon and bromadiolone in Xenopus laevis embryos. Neuro Endocrinol Lett. 2015 Jan; 36(Suppl 1): 114-119 ...
The CCBIO Research School for Cancer Studies (RSCS), which was officially opened in September 2014, focuses on translational cancer research and innovation, including international exchange and mobility as well as ethical-, legal- and societal aspects of cancer research and treatment. The research school courses are available for all interested students within the field of cancer research. The RSCS is directed by Professor and Vice-Rector Anne Christine Johannessen in collaboration with the Director of CCBIO.
Hi, I live on the island of Oahu and want to build a solid sailing boat to go island hopping. Im looking for a good sub 16 footer( due to space constraints it cant be bigger) capable of handling the rough seas here. Any suggestions?
Lux Paper found in: LUX Swatchbook, 11 x 17 Paper, 11 x 17 Paper, 11 x 17 Paper, 11 x 17 Paper, 11 x 17 Paper, 11 x 17 Paper, 11 x 17 Paper, 11 x 17..
HPLC Application #21831: Simeconazole on Lux 5µm Cellulose-2 in RP. Column used: Lux® 5 µm Cellulose-2, LC Column 250 x 4.6 mm, Ea Part#: 00G-4457-E0
Company direct No. : +39 0535 1812757 - Fax No : +39 0535 1812744 - [email protected] - PEC: [email protected] Registered Office: Medax Srl Unipersonale Via R. Piva, 1/A - 46025 - Poggio Rusco (MN) - ...
1. Asuncion, I. Bioluminescence and the future of lighting. October 2012. Available from: ,http://business.inquirer.net/88268/bioluminescence-and-the-future-of-lighting, 2. Belkin, S., Genin, A., Lonescu, M., and Zarubin, M. Bacterial bioluminescence as a lure for marine zooplankton and fish. PNAS 2012 109 (3) 853-857. Available from : ,http://www.pnas.org/content/109/3/853.full.pdf+html, 3. Boyle, R. Bacterial Lamp Can Eat Your Sewage and Light Up Your House. November 2011. Available from: ,http://www.popsci.com/technology/article/2011-11/fed-human-waste-luminous-bacteria-can-light-your-house, 4. Case. J., Haddock. S., Moline. M. January 2010. Bioluminescence in the Sea. Annual Review of Marine Science. Vol. 2: 443-493. Available from: , http://arjournals.annualreviews.org, 5. Charrier, T., Durand, M., Affi, M., Jouanneau, S., Gezekel, H., and Thouand, G.(2010). Bacterial Bioluminescent Biosensor Characterisation for On-line Monitoring of Heavy Metals Pollutions in Waste Water Treatment Plant ...
Island of Oahu is about 7 hours flight away from Tokyo. Volcanic island surrounded by coral reef formed over million years create an environment ideal for
These groups arent just diverse, but unexpectedly so. In the relatively short time theyve been around, theyve accumulated far more species than is normal, and far more so than lineages that got together with glowing bacteria. Why?. Davis thinks its because they can exert greater control over their light. While ponyfishes have to rely on body parts that obscure the continuous glow of their microbes, lanternfishes and dragonfishes can turn their glows on or off, using nerves that feed into their light organs. That means they can flash and pulse. They can use their light not just to lure prey or hide from predators, but to communicate with each other.. Many scientists think that deep-sea fish could use bioluminescence as badges of identity, allowing them to recognises others of their own kind and to mate with partners of the right species. This might also explain why these fish became so extraordinarily diverse, in an open world with no obvious features like mountains or rivers to separate ...
of the Twenry.fifth Infantry en the Logan this morning. Bottom Bight, the Jransnert Sherldah. docked on the wa side of the harbor this morning. : Photos by A. R Cnrrey, Jr ...
whom ever designed this werb sight should be fired , ask for tickets and tours no luck tells me not enough informkation . what other information is required to get a list of the daily prices to things on oahu. like pearl harbor and polinesean cultu8re center and luos I think tis is one of the hardest sites to navigate can not find anything i want to find.last year i had no problem getting a list of prices to activityus on oahu.. ...
Narges Bani Asadi Analyzing and interpreting whole human -omes Company: Bina Technologies Title: Cofounder and CEO Bina Technologies launched in 2011 planning to attack one of the major...
Lea ain nu ahte Sámedikki stuorimus bellodat lea «Ii dieđe», go sii livčče ožžon badjel 33 proseantta jienasteddjiin Sentio Research bellodatbaromehtera vuođul.
My late 2013 13 rMBP has a really annoying mura in the lower right quadrant of the display. The original display was replaced under warranty when the...
The species page of Trichophyton rubrum CBS 118892. Also known as Trichophyton rubrum var. raubitschekii, Trichophyton raubitschekii, Trichophyton megninii, Trichophyton kanei, Trichophyton fischeri, Epidermophyton rubrum. Information about genome files, completeness, GC-content, size, N50-values, and sequencing methods are listed.
If you wish to gain victory over passions and easily put to flight the hordes of mental aliens, collect yourself inwardly with Gods help by prayer and, descending into the depths of your heart, find there those three strong giants of the devil -- I mean forgetfulness, indifference or laziness, and ignorance, the food on which all other passions feed and act, live and grow strong in self-indulgent hearts and unpunished souls. With strict attention to yourself and a sober mind, and with help from above, you will certainly find these evil passions, unknown and not even suspected by others, yet more pernicious than the rest; you will find them by the weapons of righteousness which are their contrary. These weapons are memory of the good, the source of all blessings, enlightened knowledge, by which a soul kept in sobriety chases away the darkness of ignorance, and a lively zeal, which rouses the soul and leads it to salvation. Thereupon, armed with these weapons of virtue, accompanied by every ...
Dorsal spines (total): 2. 12 mesial gill rakers on first arch; eight scales below origin of second dorsal fin; no bulblike swelling of light organ, anterior dermal window represented by a small scaleless area between midbases of pelvic fins; pores of sensory canals on head small but present; color gray-brown, fins dark. Pelvic fins with long filamentous ray extending to about 13th anal ray. ...
... is an acute infectious disease of the small intestine caused by the bacterium Vibrio cholerae. It is usually spread through contaminated water...
Infectious disease caused by intestinal infection with the bacterium Vibrio cholerae, characterized by severe diarrhea and vomiting, leading to dehydration ...
The gut microbiota consists of a diverse community of microbes, living within the digestive tracts of humans, animals, and insects. While some microbes can cause infectious diseases, other microbes are vital for the development and physiology of the host. Although it is known that microbes affect host health and development, the colonization dynamics of these microbes are still unknown. In the Guillemin lab, we study these host-microbe interactions using zebrafish as a model organism to understand how microbes colonize the host gut. Aeromonas is a normal bacterial resident of the zebrafish gut that we recently discovered produces an uncharacterized double cache domain containing protein ZOR0001_03237 (3237). This protein, 3237, affects the colonization of Aeromonas in zebrafish by decreasing the rate of colonization. To further explore how 3237 could be involved in colonization, we investigated the predicted structure for clues. Based on sequence, 3237 is hypothesized to have a periplasmic ...

Genome-level homology and phylogeny of Vibrionaceae (Gammaproteobacteria: Vibrionales) with three new complete genome sequences...Genome-level homology and phylogeny of Vibrionaceae (Gammaproteobacteria: Vibrionales) with three new complete genome sequences...

From the innermost circle: S. oneidensis, P. profundum, A. salmonicida, A. fischeri ES, A. fischeri MJ, V. anguillarum, V. ... Aliivibrio logei, formerly Vibrio logei and Photobacterium logei, is the predominant light-organ symbiont of squids in the ... From the innermost circle: S. oneidensis, P. profundum, A. salmonicida, A. fischeri ES, A. fischeri MJ, V. anguillarum, V. ... SKA34, A. logei, A. salmonicida, A. fischeri ES114, V. nigripulchritudo, V. mediterranei, V. metschnikovii, V. anguillarum, V. ...
more infohttps://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-13-80

Aliivibrio fischeri (Beijerinck) Urbanczyk et al. ATCC ® 700601D-5Aliivibrio fischeri (Beijerinck) Urbanczyk et al. ATCC ® 700601D-5

Genomic DNA from Aliivibrio fischeri strain ES114 TypeStrain=False Application: ... Aliivibrio fischeri (Beijerinck) Urbanczyk et al. (ATCC® 700601D-5™) Strain Designations: Genomic DNA fromAliivibrio fischeri ... Vibrio fischeri (Beijerinck) Lehmann and Neumann Strain Designations Genomic DNA fromAliivibrio fischeri strain ES114 [ATCC® ... Aliivibrio fischeri (Beijerinck) Urbanczyk et al. ATCC® 700601D-5™ dried Total DNA: At least 5 µg in 1X TE buffer.. OD260/OD280 ...
more infohttps://www.atcc.org/en/Global/Products/D/F/5/8/700601D-5.aspx

KEGG PATHWAY: Nucleotide excision repair - Aliivibrio fischeri ES114KEGG PATHWAY: Nucleotide excision repair - Aliivibrio fischeri ES114

Nucleotide excision repair - Aliivibrio fischeri ES114 [ Pathway menu , Organism menu , Pathway entry , Download KGML , Show ...
more infohttp://www.genome.jp/kegg-bin/show_pathway?vfi03420

Aliivibrio fischeri - WikipediaAliivibrio fischeri - Wikipedia

Vibrio salmonicida and Vibrio wodanis as Aliivibrio fischeri gen. nov., comb. nov., Aliivibrio logei comb. nov., Aliivibrio ... Aliivibrio fischeri is a Gram-negative, rod-shaped bacterium found globally in marine environments. A. fischeri has ... In 2014, Hawaiʻian State Senator Glenn Wakai submitted SB3124 proposing Aliivibrio fischeri as the state microbe of Hawaiʻi. ... The genome for A. fischeri also carries mobile genetic elements. Planktonic A. fischeri bacteria are found in very low ...
more infohttps://en.wikipedia.org/wiki/Aliivibrio_fischeri

ureA - Urease subunit gamma - Aliivibrio fischeri (strain MJ11) - ureA gene & proteinureA - Urease subunit gamma - Aliivibrio fischeri (strain MJ11) - ureA gene & protein

Aliivibrio fischeri (strain MJ11) (Vibrio fischeri). ,p>This subsection of the ,a href="http://www.uniprot.org/help/names_and_ ... sp,B5FBC8,URE3_ALIFM Urease subunit gamma OS=Aliivibrio fischeri (strain MJ11) OX=388396 GN=ureA PE=3 SV=1 ... cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › AliivibrioAliivibrio ...
more infohttps://www.uniprot.org/uniprot/B5FBC8

Aliivibrio fischeri | Profiles RNSAliivibrio fischeri | Profiles RNS

"Aliivibrio fischeri" by people in this website by year, and whether "Aliivibrio fischeri" was a major or minor topic of these ... "Aliivibrio fischeri" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical ... A species of gram-negative bacteria in the genus ALIIVIBRIO, which exhibits LUMINESCENCE. A. fischeri is found in a symbiotic ... Below are the most recent publications written about "Aliivibrio fischeri" by people in Profiles. ...
more infohttp://profiles.ouhsc.edu/display/67504

By Organization / Company Page 1By Organization / Company Page 1

Aliivibrio fischeri (Beijerinck) Urbanczyk et al. (ATCC® 49387-MINI-PACK™) ATCC® Number: 49387-MINI-PACK™ Deposited As ... Aliivibrio fischeri (Beijerinck) Urbanczyk et al. (ATCC® 49387™) ATCC® Number: 49387™ Deposited As Photobacterium phosphoreum ( ...
more infohttps://www.atcc.org/Products/Quality_Control_Strains/By_Organization__Company.aspx?dsNav=Ro:90,Ns:P_Product_Name%7C101%7C1%7C,Nrc:id-1000507-dynrank-disabled%7Cid-1000568-dynrank-disabled&hasShowAll=1000507%7C1000568%7C

By Organization / Company Page 1By Organization / Company Page 1

Aliivibrio fischeri (Beijerinck) Urbanczyk et al. (ATCC® 49387-MINI-PACK™) ATCC® Number: 49387-MINI-PACK™ Deposited As ... Aliivibrio fischeri (Beijerinck) Urbanczyk et al. (ATCC® 49387™) ATCC® Number: 49387™ Deposited As Photobacterium phosphoreum ( ...
more infohttps://atcc.org/Products/Quality_Control_Strains/By_Organization__Company.aspx?dsNav=Ns:P_Product_Name%7C101%7C1%7C,N:4294955970&slp=1

LMG 4412 Strain Passport - StrainInfoLMG 4412 Strain Passport - StrainInfo

Aliivibrio fischeri 16S ribosomal RNA gene, partial sequence. ATCC 14546 2010/09/28. 1471. ...
more infohttp://www.straininfo.net/strains/22389

CHEMBL34833 Compound Report CardCHEMBL34833 Compound Report Card

Aliivibrio fischeri 1.000 CHEMBL5077 Butyrylcholinesterase Equus caballus 1.000 CHEMBL5763 Cholinesterase Equus caballus 1.000 ...
more infohttps://www.ebi.ac.uk/chembl/compound/inspect/CHEMBL34833

CHEMBL34833 Compound Report CardCHEMBL34833 Compound Report Card

Aliivibrio fischeri 0.998 CHEMBL1628461 Oxoeicosanoid receptor 1 Homo sapiens 0.997 CHEMBL5805 Toll-like receptor 8 Homo ...
more infohttps://www.ebi.ac.uk/chembldb/index.php/compound/inspect/CHEMBL34833

RCSB PDB - Protein Feature View 









 - Major NAD(P)H-flavin oxidoreductase - P46072 (FRA1 ALIFS)RCSB PDB - Protein Feature View - Major NAD(P)H-flavin oxidoreductase - P46072 (FRA1 ALIFS)

P46072 - Aliivibrio fischeri 3 ×Close. Find Sequence Motif Find. ×Close. Map Chromosome Coordinate to Protein. ...
more infohttp://www.rcsb.org/pdb/protein/P46072

NCIMB 1510 Strain Passport - StrainInfoNCIMB 1510 Strain Passport - StrainInfo

Aliivibrio fischeri. all known species names for this strain. Aliivibrio fischeri. Vibrio fischeri. ...
more infohttp://www.straininfo.net/strains/22411

Localization of EsAP activity in intact juvenile light  | Open-iLocalization of EsAP activity in intact juvenile light | Open-i

Aliivibrio fischeri/physiology*. *Alkaline Phosphatase/genetics/metabolism*. *Decapodiformes/enzymology*/genetics/microbiology/ ... C and D) Localization of V. fischeri producing red fluorescent protein (RFP). Arrows indicate where the AP activity and ... C and D) Localization of V. fischeri producing red fluorescent protein (RFP). Arrows indicate where the AP activity and ... Unlabelled: The synergistic activity of Vibrio fischeri lipid A and the peptidoglycan monomer (tracheal cytotoxin [TCT]) ...
more infohttps://openi.nlm.nih.gov/detailedresult.php?img=PMC3569863_mbo0021212690008&req=4

Saliva and bacteria, SEM - Stock Image C026/7515 - Science Photo LibrarySaliva and bacteria, SEM - Stock Image C026/7515 - Science Photo Library

Embedded in the biofilm are Aliivibrio fischeri bacteria (rod-shaped). - Stock Image C026/7515 ... Keywords: aliivibrio fischeri, bacteria, bacterial, bacteriology, bacterium, biofilm, biological, biology, black background, ... Embedded in the biofilm are Aliivibrio fischeri bacteria (rod-shaped).. Release details: Model release not required. Property ...
more infohttp://www.sciencephoto.com/media/690493/view

Quorum sensingQuorum sensing

Aliivibrio fischeri. Quorum sensing was first observed in Aliivibrio fischeri, a bioluminescent bacterium that lives as a ... The YenR and YenI proteins produced by the gammaproteobacterium Yersinia enterocolitica are similar to Aliivibrio fischeri LuxR ... For instance, the bioluminescent luciferase produced by Vibrio fischeri would not be visible if it were produced by a single ... By using quorum sensing to limit the production of luciferase to situations when cell populations are large, V. fischeri cells ...
more infohttps://ipfs.io/ipfs/QmXoypizjW3WknFiJnKLwHCnL72vedxjQkDDP1mXWo6uco/wiki/Quorum_sensing.html

Sensors | Free Full-Text | Drift Removal for Improving the Accuracy of Gait Parameters Using Wearable Sensor SystemsSensors | Free Full-Text | Drift Removal for Improving the Accuracy of Gait Parameters Using Wearable Sensor Systems

Microencapsulated Aliivibrio fischeri in Alginate Microspheres for Monitoring Heavy Metal Toxicity in Environmental Waters ...
more infohttp://www.mdpi.com/1424-8220/14/12/23230

Vibrio azasii - WikipediaVibrio azasii - Wikipedia

It iss somehow related to Aliivibrio fischeri.[citation needed] It is native to the Red Sea, and lives in symbiosis with big- ...
more infohttps://en.wikipedia.org/wiki/Vibrio_azasii

Nomenclatural changes validly published (or published) in the 2007 issues of the IJSEMNomenclatural changes validly published (or published) in the 2007 issues of the IJSEM

Aliivibrio Urbanczyk et al. 2007, gen. nov. (Part 12) Aliivibrio fischeri (Beijerinck 1889) Urbanczyk et al. 2007, comb. nov. ... Aliivibrio wodanis (Lunder et al. 2000) Urbanczyk et al. 2007, comb. nov. - Basonym: Vibrio wodanis Lunder et al. 2000. (Part ... Aliivibrio logei (Harwood et al. 1980) Urbanczyk et al. 2007, comb. nov. - Basonym: Photobacterium logei (ex Bang et al. 1978) ... Aliivibrio salmonicida (Egidius et al. 1986) Urbanczyk et al. 2007, comb. nov. - Basonym: Vibrio salmonicida Egidius et al. ...
more infohttp://www.bacterio.net/-changes-2007.html

Green Pages · The Global Directory for Environmental Technology › Current ListingsGreen Pages · The Global Directory for Environmental Technology › Current Listings

Toxicity Test Kits, Aliivibrio fischeri (formerly Vibrio f.), luminescence, Microbiotests, ATP Test Kits, Algal Toxin Tests. ...
more infohttp://www.eco-web.com/reg/

Green Pages · The Global Directory for Environmental Technology › Current ListingsGreen Pages · The Global Directory for Environmental Technology › Current Listings

Toxicity Test Kits, Aliivibrio fischeri (formerly Vibrio f.), luminescence, Microbiotests, ATP Test Kits, Algal Toxin Tests. ...
more infohttps://eco-web.com/reg/index.html

Microbial chatter: how bacteria talk to each other - Ezine - separationsNOW.comMicrobial chatter: how bacteria talk to each other - Ezine - separationsNOW.com

Aliivibrio fischeri uses a group of chemicals called acylated homoserine lactones, or AHLs, as auto-inducers. These chemicals ... For example, the bioluminescence of Aliivibrio fischeri, a rod-shaped bacterium famous for its green glow, is only activated ...
more infohttp://www.separationsnow.com/hplc/details/ezine/154530cc1e4/Microbial-chatter-how-bacteria-talk-to-each-other.html

Author: Lomba, Laura - PubAg Search ResultsAuthor: 'Lomba, Laura' - PubAg Search Results

The acute ecotoxic effects of ibuprofen and omeprazole and their mixture have been measured using Aliivibrio fischeri as a ... Vibrio fischeri; ecotoxicology; ibuprofen; median effective concentration; prediction; risk assessment; toxicity. Abstract:. ... Daphnia magna; Vibrio fischeri; alcohols; bacteria; biomass; boiling point; ecotoxicology; environmental fate; furfural; ... Danio rerio; Daphnia magna; Vibrio fischeri; biomass; ecotoxicology; glycerol; hazard characterization; ionic liquids; models; ...
more infohttps://pubag.nal.usda.gov/?q=%22Lomba%2C+Laura%22&search_field=author
  • By using quorum sensing to limit the production of luciferase to situations when cell populations are large, V. fischeri cells are able to avoid wasting energy on the production of useless product. (ipfs.io)
  • Aliivibrio fischeri" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (ouhsc.edu)
  • This graph shows the total number of publications written about "Aliivibrio fischeri" by people in this website by year, and whether "Aliivibrio fischeri" was a major or minor topic of these publications. (ouhsc.edu)