Aliivibrio fischeri: A species of gram-negative bacteria in the genus ALIIVIBRIO, which exhibits LUMINESCENCE. A. fischeri is found in a symbiotic relationship with the SQUID Euprymna scolopes.Aliivibrio salmonicida: A species of gram-negative bacteria in the genus ALIIVIBRIO. Aliivibrio salmonicida is the causative agent of cold water vibriosis, a HEMORRHAGIC SEPTICEMIA of ATLANTIC SALMON.Aliivibrio: A genus of gram-negative, rod-shaped bacteria in the family VIBRIONACEAE. They are found in the marine environment often associated with animals.Aliivibrio Infections: Infections with bacteria of the genus ALIIVIBRIO.Vibrionaceae: A family of gram-negative bacteria whose members predominate in the bacterial flora of PLANKTON; FISHES; and SEAWATER. Some members are important pathogens for humans and animals.Vibrio: A genus of VIBRIONACEAE, made up of short, slightly curved, motile, gram-negative rods. Various species produce cholera and other gastrointestinal disorders as well as abortion in sheep and cattle.Decapodiformes: A superorder of CEPHALOPODS comprised of squid, cuttlefish, and their relatives. Their distinguishing feature is the modification of their fourth pair of arms into tentacles, resulting in 10 limbs.Gadiformes: An order of fish including the families Gadidae (cods), Macrouridae (grenadiers), and hakes. The large Gadidae family includes cod, haddock, whiting, and pollock.Symbiosis: The relationship between two different species of organisms that are interdependent; each gains benefits from the other or a relationship between different species where both of the organisms in question benefit from the presence of the other.Animal Structures: Organs and other anatomical structures of non-human vertebrate and invertebrate animals.Luminescent Measurements: Techniques used for determining the values of photometric parameters of light resulting from LUMINESCENCE.Luminescence: Emission of LIGHT when ELECTRONS return to the electronic ground state from an excited state and lose the energy as PHOTONS. It is sometimes called cool light in contrast to INCANDESCENCE. LUMINESCENT MEASUREMENTS take advantage of this type of light emitted from LUMINESCENT AGENTS.Nucleoside-Phosphate Kinase: An enzyme that catalyzes reversible reactions of a nucleoside triphosphate, e.g., ATP, with a nucleoside monophosphate, e.g., UMP, to form ADP and UDP. Many nucleoside monophosphates can act as acceptor while many ribo- and deoxyribonucleoside triphosphates can act as donor. EC 2.7.4.4.Bivalvia: A class in the phylum MOLLUSCA comprised of mussels; clams; OYSTERS; COCKLES; and SCALLOPS. They are characterized by a bilaterally symmetrical hinged shell and a muscular foot used for burrowing and anchoring.4-Butyrolactone: One of the FURANS with a carbonyl thereby forming a cyclic lactone. It is an endogenous compound made from gamma-aminobutyrate and is the precursor of gamma-hydroxybutyrate. It is also used as a pharmacological agent and solvent.Bacterial Proteins: Proteins found in any species of bacterium.Neosartorya: A genus of ascomycetous fungi in the family Trichocomaceae, order EUROTIALES. Some species can cause opportunistic infections in humans, similar to its anamorph ASPERGILLUS.Rec A Recombinases: A family of recombinases initially identified in BACTERIA. They catalyze the ATP-driven exchange of DNA strands in GENETIC RECOMBINATION. The product of the reaction consists of a duplex and a displaced single-stranded loop, which has the shape of the letter D and is therefore called a D-loop structure.Photobacterium: A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria that are common in the marine environment and on the surfaces and in the intestinal contents of marine animals. Some species are bioluminescent and are found as symbionts in specialized luminous organs of fish.DNA, Ribosomal: DNA sequences encoding RIBOSOMAL RNA and the segments of DNA separating the individual ribosomal RNA genes, referred to as RIBOSOMAL SPACER DNA.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Quorum Sensing: A phenomenon where microorganisms communicate and coordinate their behavior by the accumulation of signaling molecules. A reaction occurs when a substance accumulates to a sufficient concentration. This is most commonly seen in bacteria.Phylogeny: The relationships of groups of organisms as reflected by their genetic makeup.DNA-Directed RNA Polymerases: Enzymes that catalyze DNA template-directed extension of the 3'-end of an RNA strand one nucleotide at a time. They can initiate a chain de novo. In eukaryotes, three forms of the enzyme have been distinguished on the basis of sensitivity to alpha-amanitin, and the type of RNA synthesized. (From Enzyme Nomenclature, 1992).Gene Expression Regulation, Bacterial: Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.RNA, Ribosomal, 16S: Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.Pantoea: A genus of gram-negative, facultatively anaerobic, straight rods which are motile by peritrichous flagella. Most strains produce a yellow pigment. This organism is isolated from plant surfaces, seeds, soil, and water, as well as from animals and human wounds, blood, and urine. (From Bergey's Manual of Determinative Bacteriology, 9th ed)Urease: An enzyme that catalyzes the conversion of urea and water to carbon dioxide and ammonia. EC 3.5.1.5.Urea: A compound formed in the liver from ammonia produced by the deamination of amino acids. It is the principal end product of protein catabolism and constitutes about one half of the total urinary solids.Proton-Translocating ATPases: Multisubunit enzymes that reversibly synthesize ADENOSINE TRIPHOSPHATE. They are coupled to the transport of protons across a membrane.Cetylpyridinium: Cationic bactericidal surfactant used as a topical antiseptic for skin, wounds, mucous membranes, instruments, etc.; and also as a component in mouthwash and lozenges.Databases, Chemical: Databases devoted to knowledge about specific chemicals.Databases, Pharmaceutical: Databases devoted to knowledge about PHARMACEUTICAL PRODUCTS.Pyridinium CompoundsDrug Discovery: The process of finding chemicals for potential therapeutic use.Anti-Infective Agents, Local: Substances used on humans and other animals that destroy harmful microorganisms or inhibit their activity. They are distinguished from DISINFECTANTS, which are used on inanimate objects.Mouthwashes: Solutions for rinsing the mouth, possessing cleansing, germicidal, or palliative properties. (From Boucher's Clinical Dental Terminology, 4th ed)Biometric Identification: A method of differentiating individuals based on the analysis of qualitative or quantitative biological traits or patterns. This process which has applications in forensics and identity theft prevention includes DNA profiles or DNA fingerprints, hand fingerprints, automated facial recognition, iris scan, hand geometry, retinal scan, vascular patterns, automated voice pattern recognition, and ultrasound of fingers.Radio Frequency Identification Device: Machine readable patient or equipment identification device using radio frequency from 125 kHz to 5.8 Ghz.Doping in Sports: Illegitimate use of substances for a desired effect in competitive sports. It includes humans and animals.Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.Bacterial Typing Techniques: Procedures for identifying types and strains of bacteria. The most frequently employed typing systems are BACTERIOPHAGE TYPING and SEROTYPING as well as bacteriocin typing and biotyping.FMN Reductase: An enzyme that utilizes NADH or NADPH to reduce FLAVINS. It is involved in a number of biological processes that require reduced flavin for their functions such as bacterial bioluminescence. Formerly listed as EC 1.6.8.1 and EC 1.5.1.29.Databases, Protein: Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.Internet: A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.User-Computer Interface: The portion of an interactive computer program that issues messages to and receives commands from a user.Software: Sequential operating programs and data which instruct the functioning of a digital computer.Proteins: Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.Sequence Analysis, Protein: A process that includes the determination of AMINO ACID SEQUENCE of a protein (or peptide, oligopeptide or peptide fragment) and the information analysis of the sequence.Acceleration: An increase in the rate of speed.Clothing: Fabric or other material used to cover the body.Monitoring, Ambulatory: The use of electronic equipment to observe or record physiologic processes while the patient undergoes normal daily activities.Gait: Manner or style of walking.Transducers: Any device or element which converts an input signal into an output signal of a different form. Examples include the microphone, phonographic pickup, loudspeaker, barometer, photoelectric cell, automobile horn, doorbell, and underwater sound transducer. (McGraw Hill Dictionary of Scientific and Technical Terms, 4th ed)Algorithms: A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.Posture: The position or attitude of the body.

Microbial factor-mediated development in a host-bacterial mutualism. (1/147)

Tracheal cytotoxin (TCT), a fragment of the bacterial surface molecule peptidoglycan (PGN), is the factor responsible for the extensive tissue damage characteristic of whooping cough and gonorrhea infections. Here, we report that Vibrio fischeri also releases TCT, which acts in synergy with lipopolysaccharide (LPS) to trigger tissue development in its mutualistic symbiosis with the squid Euprymna scolopes. As components of PGN and LPS have commonly been linked with pathogenesis in animals, these findings demonstrate that host interpretation of these bacterial signal molecules is context dependent. Therefore, such differences in interpretation can lead to either inflammation and disease or to the establishment of a mutually beneficial animal-microbe association.  (+info)

Complete genome sequence of Vibrio fischeri: a symbiotic bacterium with pathogenic congeners. (2/147)

Vibrio fischeri belongs to the Vibrionaceae, a large family of marine gamma-proteobacteria that includes several dozen species known to engage in a diversity of beneficial or pathogenic interactions with animal tissue. Among the small number of pathogenic Vibrio species that cause human diseases are Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus, the only members of the Vibrionaceae that have had their genome sequences reported. Nonpathogenic members of the genus Vibrio, including a number of beneficial symbionts, make up the majority of the Vibrionaceae, but none of these species has been similarly examined. Here we report the genome sequence of V. fischeri ES114, which enters into a mutualistic symbiosis in the light organ of the bobtail squid, Euprymna scolopes. Analysis of this sequence has revealed surprising parallels with V. cholerae and other pathogens.  (+info)

Magnesium promotes flagellation of Vibrio fischeri. (3/147)

The bacterium Vibrio fischeri requires bacterial motility to initiate colonization of the Hawaiian squid Euprymna scolopes. Once colonized, however, the bacterial population becomes largely unflagellated. To understand environmental influences on V. fischeri motility, we investigated migration of this organism in tryptone-based soft agar media supplemented with different salts. We found that optimal migration required divalent cations and, in particular, Mg2+. At concentrations naturally present in seawater, Mg2+ improved migration without altering the growth rate of the cells. Transmission electron microscopy and Western blot experiments suggested that Mg2+ addition enhanced flagellation, at least in part through an effect on the steady-state levels of flagellin protein.  (+info)

Vibrio fischeri uses two quorum-sensing systems for the regulation of early and late colonization factors. (4/147)

Vibrio fischeri possesses two quorum-sensing systems, ain and lux, using acyl homoserine lactones as signaling molecules. We have demonstrated previously that the ain system activates luminescence gene expression at lower cell densities than those required for lux system activation and that both systems are essential for persistent colonization of the squid host, Euprymna scolopes. Here, we asked whether the relative contributions of the two systems are also important at different colonization stages. Inactivation of ain, but not lux, quorum-sensing genes delayed initiation of the symbiotic relationship. In addition, our data suggest that lux quorum sensing is not fully active in the early stages of colonization, implying that this system is not required until later in the symbiosis. The V. fischeri luxI mutant does not express detectable light levels in symbiosis yet initiates colonization as well as the wild type, suggesting that ain quorum sensing regulates colonization factors other than luminescence. We used a recently developed V. fischeri microarray to identify genes that are controlled by ain quorum sensing and could be responsible for the initiation defect. We found 30 differentially regulated genes, including the repression of a number of motility genes. Consistent with these data, ain quorum-sensing mutants displayed an altered motility behavior in vitro. Taken together, these data suggest that the sequential activation of these two quorum-sensing systems with increasing cell density allows the specific regulation of early colonization factors (e.g., motility) by ain quorum sensing, whereas late colonization factors (e.g., luminescence) are preferentially regulated by lux quorum sensing.  (+info)

Identifying components of the NF-kappaB pathway in the beneficial Euprymna scolopes-Vibrio fischeri light organ symbiosis. (5/147)

The Toll/NF-kappaB pathway is a common, evolutionarily conserved innate immune pathway that modulates the responses of animal cells to microbe-associated molecular patterns (MAMPs). Because MAMPs have been implicated as critical elements in the signaling of symbiont-induced development, an expressed sequence tag library from the juvenile light organ of Euprymna scolopes was used to identify members of the Toll/NF-kappaB pathway. Full-length transcripts were identified by using 5' and 3' RACE PCR. Seven transcripts critical for MAMP-induced triggering of the Toll/NF-kappaB phosphorylation cascade have been identified, including receptors, signal transducers, and a transcription factor. Further investigations should elucidate the role of the Toll/NF-kappaB pathway in the initiation of the beneficial symbiosis between E. scolopes and Vibrio fischeri.  (+info)

The LuxR receptor: the sites of interaction with quorum-sensing signals and inhibitors. (6/147)

The function of LuxR homologues as quorum sensors is mediated by the binding of N-acyl-L-homoserine lactone (AHL) signal molecules to the N-terminal receptor site of the proteins. In this study, site-directed mutagenesis was carried out of the amino acid residues comprising the receptor site of LuxR from Vibrio fischeri, and the ability of the L42A, L42S, Y62F, W66F, D79N, W94D, V109D, V109T and M135A LuxR mutant proteins to activate green fluorescent protein expression from a P(luxI) promoter was measured. X-ray crystallographic studies of the LuxR homologue TraR indicated that residues Y53 and W57 form hydrogen bonds to the 1-carbonyl group and the ring carbonyl group, respectively, of the cognate AHL signal. Based on the activity and signal specificity of the LuxR mutant proteins, and on molecular modelling, a model is suggested in which Y62 (corresponding to Y53 in TraR) forms a hydrogen bond with the ring carbonyl group rather than the 1-carbonyl group, while W66 (corresponding to W57 in TraR) forms a hydrogen bond to the 1-carbonyl group. This flips the position of the acyl side chain in the LuxR/signal molecule complex compared to the TraR/signal molecule complex. Halogenated furanones from the marine alga Delisea pulchra and the synthetic signal analogue N-(sulfanylacetyl)-L-homoserine lactone can block quorum sensing. The LuxR mutant proteins were insensitive to inhibition by N-(propylsulfanylacetyl)-L-homoserine lactone. In contrast, the mutations had only a minor effect on the sensitivity of the proteins to halogenated furanones, and the data strongly suggest that these compounds do not compete in a 'classic' way with N-3-oxohexanoyl-L-homoserine lactone for the binding site. Based on modelling and experimental data it is suggested that these compounds bind in a non-agonist fashion.  (+info)

New rfp- and pES213-derived tools for analyzing symbiotic Vibrio fischeri reveal patterns of infection and lux expression in situ. (7/147)

Genetically altered or tagged Vibrio fischeri strains can be observed in association with their mutualistic host Euprymna scolopes, providing powerful experimental approaches for studying this symbiosis. Two limitations to such in situ analyses are the lack of suitably stable plasmids and the need for a fluorescent tag that can be used in tandem with green fluorescent protein (GFP). Vectors previously used in V. fischeri contain the p15A replication origin; however, we found that this replicon is not stable during growth in the host and is retained by fewer than 20% of symbionts within a day after infection. In contrast, derivatives of V. fischeri plasmid pES213 were retained by approximately 99% of symbionts even 3 days after infection. We therefore constructed pES213-derived shuttle vectors with a variety of selectable and visual markers. To include a visual tag that can be used in conjunction with GFP, we compared seven variants of the DsRed2 red fluorescent protein (RFP): mRFP1, tdimer2(12), DsRed.T3, DsRed.T4, DsRed.M1, DsRed.T3_S4T, and DsRed.T3(DNT). The last variant was brightest, displaying >20-fold more fluorescence than DsRed2 in V. fischeri. RFP expression did not detectably affect the fitness of V. fischeri, and cells were readily visualized in combination with GFP-expressing cells in mixed infections. Interestingly, even when inocula were dense enough that most E. scolopes hatchlings were infected by two strains, there was little mixing of the strains in the light organ crypts. We also used constitutive RFP in combination with the luxICDABEG promoter driving expression of GFP to visualize the spatial and temporal induction of this bioluminescence operon during symbiotic infection. Our results demonstrate the utility of pES213-based vectors and RFP for in situ experimental approaches in studies of the V. fischeri-E. scolopes symbiosis.  (+info)

Cyclo(Phe-Pro) modulates the expression of ompU in Vibrio spp. (8/147)

Vibrio vulnificus was found to produce a chemical that induced the expression of Vibrio fischeri lux genes. Electron spray ionization-mass spectrometry and 1H nuclear magnetic resonance analyses indicated that the compound was cyclo(L-Phe-L-Pro) (cFP). The compound was produced at a maximal level when cell cultures reached the onset of stationary phase. Sodium dodecyl sulfate-polyacrylamide gel analysis of the total proteins of V. vulnificus indicated that expression of OmpU was enhanced by exogenously added synthetic or purified cFP. A toxR-null mutant failed to express ompU despite the addition of cFP. The related Vibrio spp. V. cholerae, V. parahaemolyticus, and V. harveyi also produced cFP, which induced the expression of their own ompU genes. cFP also enhanced the expression in V. cholerae of the ctx genes, which are known to be regulated by ToxR. Our results suggest that cFP is a signal molecule controlling the expression of genes important for the pathogenicity of Vibrio spp.  (+info)

The mutualistic association between the squid Euprymna scolopes and the bacterium Vibrio fischeri is an emerging experimental system for the study of the influence of bacteria on animal development. Taking advantage of the ability to raise both this host and its microbial partner independently under laboratory conditions, we describe the effects of bacterial interactions on morphogenesis of the juvenile host symbiotic organ. Our results show that bacteria are essential for normal postembryonic development of the symbiotic organ, which involves changes in both the surface epithelium and the epithelial tissue within the organ where the bacterial culture will take up residence. Cell death induced by exposure to symbiotic V. fischeri results in the regression of a complex ciliated surface epithelium, a tissue that apparently functions to facilitate inoculation of the juvenile organ with the appropriate specific bacterial species. Regression of this tissue begins within hours of exposure to ...
Domain combinations containing the D-ribose-5-phosphate isomerase (RpiA), lid domain superfamily in Vibrio fischeri ES114. Domain architectures illustrate each occurrence of the D-ribose-5-phosphate isomerase (RpiA), lid domain superfamily.
Domain architecture and assignment details (superfamily, family, region, evalue) for gi|59711463|ref|YP_204239.1| from Vibrio fischeri ES114. Plus protein sequence and external database links.
Euprymna scolopes, a Hawaiian species of bioluminescent squid, harbors Vibrio fischeri as its specific light organ symbiont. The population of symbionts grew inside the adult light organ with an average doubling time of about 5 h, which produced an excess of cells that were expelled into the surrounding seawater on a diurnal basis at the beginning of each period of daylight. These symbionts, when expelled into the ambient seawater, maintain or slightly increase their numbers for at least 24 h. Hence, locations inhabited by their hosts periodically receive a daily input of symbiotic V. fischeri cells and, as a result, become significantly enriched with these bacteria. As estimated by hybridization with a species-specific luxA gene probe, the typical number of V. fischeri CFU, both in the water column and in the sediments of E. scolopes habitats, was as much as 24 to 30 times that in similar locations where squids were not observed. In addition, the number of symbiotic V. fischeri CFU in seawater ...
Coloured scanning electron micrograph (SEM) of Vibrio fischeri, Gram-negative, motile, bioluminescent, curved rod-shaped bacterium (prokaryote). Vibrio fischeri is a bioluminescent bacterium that occurs in global ocean environments. V. fischeri is most often found in a symbiotic relationship with certain deep sea marine life, such as the bobtail squid, within special light-organs.. V. fischeri causes the squid to produce a mucus substance that is secreted below the light organ on the squid. Cilia are used to draw more bacteria into this mucus matrix. The bacteria migrate into the light organ and colonize an area within it. They provide a light source for the squid to make it blend in with the ocean floor at night. Thus predators have a hard time seeing the squid. V. fischeri is a key research organism for examination of microbial bioluminescence, quorum sensing, and bacterial-animal symbiosis. Magnification: x8,000 when shortest axis printed at 25 - Stock Image C032/1744
Courtesy of: http://dels-old.nas.edu/oceans/oceans_and_human_health_part_2.shtml Introduction: This blog examines the relationship between the Hawaiian Bobtail Squid and a bioluminescent bacterium. The Hawaiian Bobtail squid acquires an enhanced ability to capture prey and avoid predators, while the bacterium receives key nutrients from residing within the squid [2]. Distribution of this aquatic relationship is limited by the habitat of the squid (Hawaii). Several bioluminescent bacteria cycle through a single squid daily. Description of Relationship: The type of relationship observed between Euprymna scolope(Hawaiian Bobtail squid) and Vibrio fischeri(bioluminescent bacterium) is unique to organisms with specialized structures called light organs. These light organs are specialized structures that have evolved to allow the organisms that house them to create light. While the evolutionary reason for this structure is unknown, many individuals hypothesize that certain marine organisms form these ...
Description: In the Hawaiian bobtail squid (pictured), scientists have found that the same bacterial toxin that causes whooping cough and gonorrhea in humans is responsible for the development of an important organ. The finding, reported in the Nov. 12 Science magazine, suggests that the role of bacteria in their host organisms depends significantly on the biological context in which they are found. In one place, a bacterium may have a harmful role; in another, it may be critical for the animals survival. The corresponding author of the Science paper is Margaret McFall-Ngai, a UW-Madison professor of medical microbiology ...
Bobtail squid (order Sepiolida) are a group of cephalopods closely related to cuttlefish. Bobtail squid tend to have a rounder mantle than cuttlefish and have no cuttlebone. They have eight suckered arms and two tentacles and are generally quite small (typical male mantle length being between 1 and 8 cm). Sepiolids live in shallow coastal waters of the Pacific Ocean and some parts of the Indian Ocean and Atlantic Ocean as well as in shallow waters on the west coast of the Cape Peninsula off South Africa. Like cuttlefish, they can swim by either using the fins on their mantle or by jet propulsion. They are also known as dumpling squid (owing to their rounded mantle) or stubby squid. Bobtail squid have a symbiotic relationship with bioluminescent bacteria (Aliivibrio fischeri), which inhabit a special light organ in the squids mantle. The bacteria are fed a sugar and amino acid solution by the squid and in return hide the squids silhouette when viewed from below by matching the amount of light ...
Genetically altered or tagged Vibrio fischeri strains can be observed in association with their mutualistic host Euprymna scolopes, providing powerful experimental approaches for studying this symbiosis. Two limitations to such in situ analyses are the lack of suitably stable plasmids and the need for a fluorescent tag that can be used in tandem with green fluorescent protein (GFP). Vectors previously used in V. fischeri contain the p15A replication origin; however, we found that this replicon is not stable during growth in the host and is retained by fewer than 20% of symbionts within a day after infection. In contrast, derivatives of V. fischeri plasmid pES213 were retained by ∼99% of symbionts even 3 days after infection. We therefore constructed pES213-derived shuttle vectors with a variety of selectable and visual markers. To include a visual tag that can be used in conjunction with GFP, we compared seven variants of the DsRed2 red fluorescent protein (RFP): mRFP1, tdimer2(12), DsRed.T3, ...
Tracheal cytotoxin (TCT) is a bacterial virulence factor in whooping cough (and other infections) that triggers leukocyte infiltration and strips airways of ciliated epithelium. However, not all host-bacterial encounters are negative. In the relationship between the glow-in-the dark squid Euprymna scolopes and its luminescent bacterial symbiont Vibrio fischeri, Koropatnick et al. have discovered that the same toxin plays a benign role. In this association, TCT still initiates apoptosis of ciliated cells and host cell infiltration. However, these cellular responses are triggered when juvenile squid first encounter V. fischeri and constitute a normal developmental stage in the generation of the specialized light organ that allows it to accommodate the incoming symbiont.. T. A. Koropatnick, J. T. Engle, M. A. Apicella, E. V. Stabb, W. E. Goldman, M. J. McFall-Ngai, Microbial factor-mediated development in a host-bacterial mutualism. Science 306, 1186-1188 (2004). [Abstract] [Full Text]. ...
Bioluminescent bacteria are light-producing bacteria that are predominantly present in sea water, marine sediments, the surface of decomposing fish and in the gut of marine animals. While not as common, bacterial bioluminescence is also found in terrestrial and freshwater bacteria. These bacteria may be free living (such as Vibrio harveyi) or in symbiosis with animals such as the Hawaiian Bobtail squid (Aliivibrio fischeri) or terrestrial nematodes (Photorhabdus luminescens). The host organisms provide these bacteria a safe home and sufficient nutrition. In exchange, the hosts use the light produced by the bacteria for camouflage, prey and/or mate attraction. Bioluminescent bacteria have evolved symbiotic relationships with other organisms in which both participants benefit close to equally. Another possible reason bacteria use luminescence reaction is for quorum sensing, an ability to regulate gene expression in response to bacterial cell density. Records of bioluminescent bacteria existed for ...
The developmental changes bobtail squid undergo when colonized by the bacteria are both dramatic and accessible for biologists to study. The bobtail squid, Euprymna scolopes, hatches as a fully formed squid. They have no larval stage. However, theres a vestige of the ciliated structures seen in other mollusks. (Cilia are like tiny hairs on the surface of individual cells.) These ciliated cells cover the surface of a pair of "appendages" in the undeveloped light organ (Koropatnick, 2014). After hatching, the bobtail squid has no association with any bacteria, and is not capable of luminescence. The light organ can only complete its development once it is colonized by a specific species of bacteria, Vibrio fischeri. Once V. fischeri is obtained from the surrounding seawater, the bacteria become suspended in mucus and migrate to the light organ. The cells smell their way! They follow a trail of chemical cues left by the squids cells (Koropatnick, 2014). Approximately 12 hours after hatching, the ...
The Genomic tRNA Database is curated by Todd Lowe and Patricia Chan. Please email [email protected] with any questions and comments. To submit corrections, please use our Bug and Request Tracking System ...
Aliivibrio fischeri ATCC ® 700601D-5™ Designation: Genomic DNA from Aliivibrio fischeri strain ES114 TypeStrain=False Application:
... - The other day I decided I wanted to make some sort of sea creature, & after a bit of research I decided the Bobtail Squid was the one for me. I wr
The relationship between the Hawaiian bobtail squid and the bacterium Vibrio fischeri is well chronicled, but writing in the current issue of the journal Proceedings of the Royal Society B, a group led by University of Wisconsin-Madison microbiologists Margaret McFall-Ngai, Edward Ruby and their colleagues adds a new wrinkle to the story.
Homologs of Toxin-Encoding Genes. Although V. fischeri is not known to be pathogenic, and strain ES114 is a beneficial symbiont, its genome carries homologs of Vibrio genes that may have toxin activity (Table 3, which is published as supporting information on the PNAS web site). As mentioned earlier, these include two CTX phage-encoded genes, zot (zona occludins toxin) and ace (accessory cholera enterotoxin), the latter of which has been found only in the V. cholerae and V. fischeri genome sequences. The proteins coded for by these genes have been shown to contribute to the structure of CTX phage (17), and their possible roles as toxins remain controversial (31, 32). At this time, it is not known whether the V. fischeri homologs of these two genes are expressed in this species, or whether they might play a role in this bacteriums symbiotic associations.. All sequenced Vibrio species carry genes encoding another putative toxin called RTX (repeats in structural toxins). RTX activity in V. ...
PAN Czytelnia Czasopism, Toxicity Of Sulcotrione Photoproducts Mixture Towards Vibrio Fischeri In The Aquatic Environment - Archives of Environmental Protection
The American Bobtail, a relative newcomer on the scene, is increasing in popularity due to its winning combination of wild appearance and friendly demeanour.. Among the few cat breeds that have originated in the United States, the American Bobtail did not receive recognition by the Cat Fanciers Association until February 2000.. American Bobtail History. The first known American Bobtail was Yodie, a stray with a stubby tail who was found by vacationers in the 1960s. Although his parentage is unknown, it was rumoured that he was a hybrid of a bobcat and a domestic cat due to his short tail and wild look. However, this is unlikely, as the males derived from such matings tend to be sterile, whereas Yodie was not. It is more likely that Yodies stumpy tail was the result of a spontaneous mutation.. Yodie was mated at first with a seal point Siamese female and the kittens were born with bobbed tails, which indicated that the bobtail gene was dominant. Yodie and his children were subsequently mated ...
Glowing bacteria inside squids use light and chemical signals to control circadian-like rhythms in the animals, according to a study to be published in mBio®. The Hawaiian bobtail squid, Euprymna ...
My current research focuses on analyzing DNA sequence determinants of gene expression in the bacteria Vibrio fischeri. Previous studies of the rrn genes in the bacterium Escherichia coli have laid a strong foundation for our understanding of gene expression. These genes have proven to be a successful model for studying transcription in E. coli; therefore I am investigating the rrn genes in other bacterial species to learn more about transcription regulation. Specifically, I use reporter gene constructs to analyze the factors involved in gene expression in V. fischeri. This work will further our understanding of the prokaryotic gene regulation. What we learn about transcription will help us to better understand the process of gene expression in general and how this process is regulated.. ...
A previously applied alanine-scanning mutagenesis that was performed with the E. coli csrA gene, used to examine the relative contribution each amino acid residue to CsrA protein function [20], provides an invaluable comparator to our spontaneous suppression screen. Our complementary screen differs in that all potential amino acid substitutions are possible, even the single native alanine residue that was not altered in the alanine scan. In our screen, an A36S substitution caused a 20-fold increase in luminescence and restored siderophore activity indicating the highly conserved A36, positioned into the hydrophobic core of the CsrA homodimer, could be critical to protein folding and 3- dimensional stability [20, 23]. Even the conservative substitution of this alanine with the non-polar and fairly compact valine (VCW2G2) altered CsrA function (Fig. 2). Also, only mutations that improve growth would likely improve fitness. Thus, mutations that map to non-conserved regions that do not contribute to ...
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Vision in fishes and crustaceans is a fascinating and understudied area. In past decades, there were far more studies on the visual systems of sea-dwelling creatures, but with the push towards applied or translational research, the number of reports in these species have dropped off, much to our detriment as one never knows where the […]. ...
This activity explores the content presented in the animated video Natures Cutest Symbiosis: The Bobtail Squid, which describes how the light produced by bacteria living inside bobtail squid helps the squid avoid its predators.. ...
Experiment is an online platform for funding and sharing scientific discoveries. Push the boundaries of knowledge in biology, chemistry, medicine, physics, computer science, paleontology, economics, engineering, neuroscience, and more.
Dan Wesson Bobtail CCO 45 ACP, Black Ceramic Coat, Night Sights. Dan Wesson 01962. Due to the popularity of the Guardians concealable profile and Commander-length slide, Dan Wesson took it a step further with the CCO - the Concealed Carry Officer - a Commander slide on an Officer frame. With the...
on my 3 element 40 meter bobtail fed at the top of the center element, it made a huge difference how it was wired... I had to connect the center conductor of the coax to the vertical elemennt and the shield to the horizontal phasing wire. It allowed me to work(and conform) my last 3 zones on 40 ...
Description. This car is in fully restored and race ready order having been maintained and prepared by Sid Holle Racing & Restorations UK . Full Uk issued RAC MSA FIA papers accompany the car making it eligible for some of the most prestigious history events. Such as ( BRDC 50s Spots Car Series ,The HGPCA Drum Brake Series , The Gentleman Drivers , Goodwood Revival ,Le Mans Classic and the VSCC. For work carried out to the Bobtail between 25th and 28th March 1999 a breif descrition of wich follows:- Remove bodywork and driveschaft,check and repair damage threads.Refit schaft& checkgear selection.Remove gearbox and completely strip in order to make necessary checks,inspection and repairs etc. Supplied and fitted new 3rd Gear. Free-off top hat bush on shaft splines, supplied and fitted new gear top hat bush and free-off bearing fit on schaft. Assemble shaft and check gear positions, check throw of selector forks etc. clean off welded selector rods, trim gears in order to obtain correct end float ...
During the 1960s, the American bobtail cat originated in the United States when a female seal point Siamese cat bred with Yodi, an male brown tabby that had an …
This wonderful DVD was made as a guide for new rabbit owners, (and some existing owners that need advice). Mostly filmed here at Bobtails Rescue Centre, this DVD offers guidance on subjects such as ...
FIG. 4. Diffusible signaling in bacteria-host associations. (A) Not only do bacteria recognize their own secreted signals (gray circles) to coordinate a response (black diamonds), but eukaryotic cells in the vicinity may also recognize these signals and produce a response. Some of the potential interactions are diagrammed. Various host cells may alter cytokine production (open triangles), mucus production (shaded gray), or other developmental events, such as apoptosis, in response to either the signal itself or the bacterial product of the signal transduction pathway. Hosts may also induce or constitutively synthesize signal-degrading enzymes. (B) Symbiotic Euprymna scolopes-V. fischeri interaction during light organ colonization. LuxA activity is dependent on AHL quorum sensing. The locations at which bacterial cell-cell signaling likely occurs are labeled as 1 and 2. Region 1 is the site of the initial attachment, or aggregation, by bacteria on the surface of the light organ. Region 2 is the ...
Members of the class Cephalopoda are gonochoric. Male and female adults usually die shortly after spawning and brooding, respectively. Mating behavior: Males perform various displays to attract potential females for copulation. During copulation, male grasp the female and inserts the hectocotylus into the females mantle cavity where fertilization usually occurs. Life cycle: Embryos hatch into planktonic stage and live for some time before they grow larger and take up a benthic existence as adults (Ref. 833). ...
Squid typically arent my thing, but I can certainly be wooed by their microbes. Example: the very awesome symbiotic relationship between the Hawaiian bobtail
Many recent studies rely on 16S rRNA-based sequencing approaches to analyze bacterial or archaeal communities found in soil and other environmental samples. While this approach is valuable for determining the relative abundances of different microbial taxa found in a given sample, it does not provide information on how the total abundances of targeted microbes differ across samples. Here we demonstrate how the simple addition of an internal standard at the DNA extraction step allows for the quantitative comparison of taxon abundances across samples. The reliability of this method was assessed in two ways. First, we spiked a dilution series of two different soils with internal standards to ascertain whether we could accurately quantify differences in cell abundances. We tested two different internal standards, adding DNA from Aliivibrio fischeri or Thermus thermophilus, bacterial taxa unlikely to be found in soil. Both standards allowed us to accurately quantify microbial abundances in soil as ...
Today in downtown Ewell we had a very nice surprise a cheque for 250. It came from BAA. Here with me now is Delia Cordell. What can you tell me Aunite Delia: Well, Dougal. We had a lovely donation from BAA thanks to one of our volunteers who works there. BAA chooses to support some of its staff good causes each year and this year decided to give our Karen 250 to pass on to us. We d like to say a big thank you to Karen for putting us forward and to BAA ...
Presence of a naturally shortened tail. Description: DNA test can identifying dogs carrying the phenotype "Natural Bobtail". Symptoms : Shortened tail, variability according to individuals: missing tail to 3/4 of tail length. Age of onset : _. Frequency : _. Gene involved : T. Tested mutation : c.295C>G. Transmission : Autosomal Dominant. Patent : _. Notes : _. PrintSend to a friendTweet WidgetPartager sur Facebook ...
The enzymes for luminescence in Vibrio fischeri are induced by the accumulation of a species-specific metabolite (autoinducer) in the culture medium. Tritium-labeled autoinducer was used to study the mechanism of autoinduction. When 3H-autoinducer was added to suspensions of V. fischeri or Escherichia coli, cellular concentrations equaled external concentrations. For V. fischeri, equilibration of 3H-autoinducer was rapid (within 20 s), and greater than 90% of the cellular tritium remained in unmodified autoinducer. When V. fischeri or E. coli cells containing 3H-autoinducer were transferred to autoinducer-free buffer, 85 to 99.5% of the radiotracer escaped from the cells, depending on the strain. Concentrations of autoinducer as low as 10 nM, which is equivalent to 1 or 2 molecules per cell, were sufficient for induction, and the maximal response to autoinducer occurred at about 200 nM. If external autoinducer concentrations were decreased to below 10 nM after induction had commenced, the ...
Recombinant Epoxyqueuosine Reductase, Cobalamine-Stimulated, Queosine Biosynthesis (QUEG) Protein (His tag). Species: Vibrio fischeri. Source: Yeast. Order product ABIN1630401.
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
TY - JOUR. T1 - Characterization of a recombinant aryl β-glucosidase from Neosartorya fischeri NRRL181. AU - Kalyani, Dayanand. AU - Lee, Kyoung-Mi. AU - Tiwari, Manish Kumar. AU - Ramachandran, Priyadharshini. AU - Kim, Hoon. AU - Kim, In-Won. AU - Jeya, Marimuthu. AU - Lee, Jung-Kul. PY - 2012. Y1 - 2012. N2 - An isolated gene from Neosartorya fischeri NRRL181 encoding a β-glucosidase (BGL) was cloned, and its nucleotide sequence was determined. DNA sequence analysis revealed an open reading frame of 1,467 bp, capable of encoding a polypeptide of 488 amino acid residues. The gene was over-expressed in Escherichia coli, and the protein was purified using nickel-nitrilotriacetic acid chromatography. The purified recombinant BGL showed a high level of catalytic activity, with V max of 886 μmol min−1 mg-protein−1 and a K m of 68 mM for p-nitrophenyl-β-d-glucopyranoside (pNPG). The optimal temperature for enzyme activity was about 40°C, and the optimal pH was about 6.0. A homology model of ...
The squid sucks in water and the current whooshes Ali and Mai closer and closer Meet Ali and Mai , Vibrio fischeri Bacterium, who live inside Sepio - a Bobtail Squid. These three creatures need each other and live in Symbiosis, helping each other to grow and survive. This story has been broken up into…
Looking for online definition of RSCS in the Medical Dictionary? RSCS explanation free. What is RSCS? Meaning of RSCS medical term. What does RSCS mean?
Why would someone want to add a fragment of dead coral to a liquid culture of bioluminescent bacteria. I thought why not. This is what happened.
Involved in the maturation of Asn-linked oligosaccharides. Progressively trims alpha-1,2-linked mannose residues from Man(9)GlcNAc(2) to produce Man(5)GlcNAc(2) (By similarity).
The Bobtail Lizard is often seen sun basking on the road - please try not to run them over as they bond with their mate for life and raise their young together.
Transposon mutants of the prototrophic V. fischeri strain ESR1 (19) were obtained by conjugative transposon mutagenesis with Escherichia coli strain S17-1 λpir (pUT mini-Tn5 Cm) (21), and selection for transconjugants was performed on a Luria broth and NaCl (LBS) agar medium (22) containing (per liter) 100 mg rifampicin, and 5 mg chloramphenicol. This procedure has been shown to cause single transposon insertions that are stable in V. fischeri (23). Amino acid auxotrophs were isolated from this pool of transposon mutants by using a streptozotocin enrichment method (24). Strains that could be complemented by the addition of a single amino acid were identified (25), and nine mutants that were auxotrophic for different amino acids were analyzed further. The growth rate and luminescence of these mutants were indistinguishable from that of strain ESR1 in SWT broth, a complex tryptone-based seawater medium (13); however, each mutant was unable to grow in the minimal-salts medium MSR, a derivative of ...
A chemical released by the bacterium, Vibrio fischeri, sends this protozoan, Salpinogoeca rosetta, into a mating swarm, demonstrating that bacteria can dri...
Five sediment cores (S1−S5) were collected from the Shima River to determine the bioavailability of trace metals (Cd, Cr, Cu, Ni, and Zn) using the modified European Community Bureau of Reference (BCR) procedure. The toxic effects of polluted sediment were assessed using the LUMISTox® bioassay with Vibrio fischeri and chemical models such as the toxicity unit (TU) of each metal, sum of all TUs (∑T ...
It iss somehow related to Aliivibrio fischeri.[citation needed] It is native to the Red Sea, and lives in symbiosis with big- ...
Aliivibrio fischeri was selected because it lives in a symbiotic relationship with the native Hawaiʻian bobtail squid, in which ... In February 2014, Senate Bill 3124 was introduced by Senator Glenn Wakai; the bill designates Aliivibrio fischeri as the State ... Aliivibrio fischeri can be found elsewhere. The combined Hawaiʻian Legislature could not agree on which microbe better suited ... and later contested in 2014 by Senator Glenn Wakai's SB3124 bill proposing Aliivibrio fischeri instead. As of December 2017[ ...
The light may be produced by the animals themselves, or by symbiotic bacteria, often Aliivibrio fischeri. Counter-illumination ... The luminescent bacterium is often Aliivibrio fischeri, as for example in the Hawaiian bobtail squid, Euprymna scolopes. ...
In 2014, Wakai proposed SB3124 which attempted to establish Aliivibrio fischeri as the state microbe of Hawaiʻi. This was ...
Vibrio salmonicida and Vibrio wodanis as Aliivibrio fischeri gen. nov., comb. nov., Aliivibrio logei comb. nov., Aliivibrio ... and Aliivibrio wodanis comb. nov". International Journal of Systematic and Evolutionary Microbiology. 57 (12): 2823-2829. doi: ... The family Vibrionaceae currently comprises eight validly published genera: Aliivibrio, Catenococcus, Enterovibrio, Grimontia, ...
For example, Aliivibrio fischeri grows favourably in environments that have temperatures between 5 to 30°C and a pH that is ... Unlike Aliivibrio fischeri, V. harveyi do not possess the luxI/luxR regulatory genes and therefore have a different mechanism ... A well known example of quorum sensing is that which occurs between Aliivibrio fischeri and its host. This process is regulated ... In bioluminescent bacteria, the reclassification of the members ofVibrio fischeri species group as a new genus, Aliivibrio, has ...
"Aliivibrio fischeri lipopolysaccharide induces developmental apoptosis, but not complete morphogenesis, of the Euprymna ... "Symbiotic role of the viable but nonculturable state of Aliivibrio fischeri in Hawaiian coastal seawater" (PDF). Archived from ... "Alterations in the protoeme of the Euprymna scolopes light organ in response to symbiotic Aliivibrio fischeri" (PDF). Archived ... "Isolation and characterization of a visibly luminous variant of Aliivibrio fischeri strain ES114 form the sepiolid squid ...
... was first observed in Aliivibrio fischeri, a bioluminescent bacterium that lives as a mutualistic symbiont in ... The YenR and YenI proteins produced by the gammaproteobacterium Yersinia enterocolitica are similar to Aliivibrio fischeri LuxR ... When A. fischeri cells are free-living (or planktonic), the autoinducer is at low concentration, and, thus, cells do not ... paradigm as defined by the Vibrio fischeri system occur in the Gram-negative Proteobacteria. A comparison between the ...
... was deferred for a year when it encountered competition from Senator Glenn Wakai's SB3124 proposing Aliivibrio fischeri. In ...
They used bacteria called Aliivibrio fischeri which glow in the dark, but the maximum lifetime of their product was three days ... "The first engagement of partners in the Euprymna scolopes-Vibrio fischeri symbiosis is a two-step process initiated by a few ...
... have a symbiotic relationship with bioluminescent bacteria (Aliivibrio fischeri), which inhabit a special light ... Symbiosis with A. fischeri from the surrounding seawater is initiated immediately upon hatching, and the bacteria's ...
... was deferred for a year when it encountered competition from Senator Glenn Wakai's SB3124 proposing Aliivibrio fischeri. 2012 ...
... and later contested in 2014 by Senator Glenn Wakai's SB3124 bill proposing Aliivibrio fischeri instead. As of December 2017[ ...
Aliivibrio fischeri and Aliivibrio salmonicida the spf gene is flanked by polA (upstream) and a sRNA gene encoding the novel ... "Expression profiling reveals Spot 42 small RNA as a key regulator in the central metabolism of Aliivibrio salmonicida". BMC ... Aliivibrio, Photobacterium and Grimontia genera). In e.g., Vibrio cholerae, Vibrio vulnificus, ... and the majority of experimental work on Spot42 has been performed in Escherichia coli and recently in Aliivibrio salmonicida. ...
Mycoplasma genitalium - a minimal organism Aliivibrio fischeri - quorum sensing, bioluminescence and animal-bacterial symbiosis ...
Quorum sensing was first studied in V. fischeri (now Aliivibrio fischeri), a marine bacterium that uses a synthase (LuxI) to ... Despite its relatedness to A. fischeri, V. harveyi lacks a LuxI/R quorum-sensing system, and instead employs a hybrid quorum- ...
Vibrio salmonicida and Vibrio wodanis as Aliivibrio fischeri gen. nov., comb. nov., Aliivibrio logei comb. nov., Aliivibrio ... Aliivibrio is a genus in the phylum Proteobacteria (Bacteria). The name Aliivibrio derives from: Latin alius, other, another, ... Aliivibrio entry in LPSN [Euzéby, J.P. (1997). "List of Bacterial Names with Standing in Nomenclature: a folder available on ... and Aliivibrio wodanis comb. nov". International Journal of Systematic and Evolutionary Microbiology. 57 (12): 2823-2829. doi: ...
Vibrio salmonicida and Vibrio wodanis as Aliivibrio fischeri gen. nov., comb. nov., Aliivibrio logei comb. nov., Aliivibrio ... Aliivibrio fischeri is a Gram-negative, rod-shaped bacterium found globally in marine environments. A. fischeri has ... In 2014, Hawaiʻian State Senator Glenn Wakai submitted SB3124 proposing Aliivibrio fischeri as the state microbe of Hawaiʻi. ... The genome for A. fischeri also carries mobile genetic elements. Planktonic A. fischeri bacteria are found in very low ...
Aliivibrio fischeri. G. *Grimontia hollisae. L. *Listonella. *Listonella anguillarum. P. *Photobacterium. *Photobacterium ...
Aliivibrio fischeri on võtmeorganism biolouminestsentsi, quorum sensingu ehk hulgatunnetuse ning bakteri ja looma vahelise ...
Aliivibrio fischeri on võtmeorganism biolouminestsentsi, quorum sensingu ehk hulgatunnetuse ning bakteri ja looma vahelise ...
Vibrio salmonicida and Vibrio wodanis as Aliivibrio fischeri gen. nov., comb. nov., Aliivibrio logei comb. nov., Aliivibrio ... Aliivibrio fischeri is a Gram-negative, rod-shaped bacterium found globally in marine environments. A. fischeri has ... In 2014, Hawaiʻian State Senator Glenn Wakai submitted SB3124 proposing Aliivibrio fischeri as the state microbe of Hawaiʻi. ... The genome for A. fischeri also carries mobile genetic elements. Planktonic A. fischeri bacteria are found in very low ...
Genomic DNA from Aliivibrio fischeri strain ES114 TypeStrain=False Application: ... Aliivibrio fischeri (Beijerinck) Urbanczyk et al. (ATCC® 700601D-5™) Strain Designations: Genomic DNA fromAliivibrio fischeri ... Vibrio fischeri (Beijerinck) Lehmann and Neumann Strain Designations Genomic DNA fromAliivibrio fischeri strain ES114 [ATCC® ... Aliivibrio fischeri (Beijerinck) Urbanczyk et al. ATCC® 700601D-5™ dried Total DNA: At least 5 µg in 1X TE buffer.. OD260/OD280 ...
Nucleotide excision repair - Aliivibrio fischeri ES114 [ Pathway menu , Organism menu , Pathway entry , Download KGML , Show ...
Aliivibrio fischeri (strain MJ11) (Vibrio fischeri). ,p>This subsection of the ,a href="http://www.uniprot.org/help/names_and_ ... sp,B5FBC8,URE3_ALIFM Urease subunit gamma OS=Aliivibrio fischeri (strain MJ11) OX=388396 GN=ureA PE=3 SV=1 ... cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › AliivibrioAliivibrio ...
"Aliivibrio fischeri" by people in this website by year, and whether "Aliivibrio fischeri" was a major or minor topic of these ... "Aliivibrio fischeri" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical ... A species of gram-negative bacteria in the genus ALIIVIBRIO, which exhibits LUMINESCENCE. A. fischeri is found in a symbiotic ... Below are the most recent publications written about "Aliivibrio fischeri" by people in Profiles. ...
Aliivibrio fischeri (Beijerinck) Urbanczyk et al. (ATCC® 49387-MINI-PACK™) ATCC® Number: 49387-MINI-PACK™ Deposited As ... Aliivibrio fischeri (Beijerinck) Urbanczyk et al. (ATCC® 49387™) ATCC® Number: 49387™ Deposited As Photobacterium phosphoreum ( ...
Aliivibrio fischeri formerly Vibrio Fischeri ), Biotoxicity, USA BioTox™ 1243-1000 WaterTox™ Standard Kit (Aliivibrio ... Included in the Aliivibrio fischeri (formally Vibrio Fischeri) WaterTox™ STD Kit:. *Freeze dried Aliivibrio fischeri reagent ... BioTox™ 1243-1000 WaterTox™ Standard Kit (Aliivibrio fischeri formerly Vibrio Fischeri ). This kit is packaged to mimic the ... Home › BioProducts › WaterTox™ Standard Kit (Aliivibrio fischeri formerly Vibrio Fischeri ), Biotoxicity, USA. ...
Aliivibrio fischeri 1.000 CHEMBL5077 Butyrylcholinesterase Equus caballus 1.000 CHEMBL5763 Cholinesterase Equus caballus 1.000 ...
Aliivibrio fischeri 0.998 CHEMBL1628461 Oxoeicosanoid receptor 1 Homo sapiens 0.997 CHEMBL5805 Toll-like receptor 8 Homo ...
Aliivibrio fischeri 16S ribosomal RNA gene, partial sequence. ATCC 14546 2010/09/28. 1471. ...
P46072 - Aliivibrio fischeri 3 ×Close. Find Sequence Motif Find. ×Close. Map Chromosome Coordinate to Protein. ...
Q5E8S6 (ENGB_ALIF1) Aliivibrio fischeri (strain ATCC 700601 / ES114) (Vibrio fischeri). Probable GTP-binding protein EngB ... Aliivibrio fischeri (strain ATCC 700601 / ES114) (Vibrio fischeri) ...
Vibrio salmonicida and Vibrio wodanis as Aliivibrio fischeri gen. nov., comb. nov., Aliivibrio logei comb. nov., Aliivibrio ... fischeri, nine (h, x, z); Aliivibrio logei, eight (i); P. damselae, one (x); Photobacterium kishitanii, 14 (a, e, j, q, t, z); ... "fischeri group," recently reclassified as members of a new genus, Aliivibrio (61), are regulatory genes, luxR and luxI, ... The lux genes of S. hanedai and S. woodyi were found to be closely related to those of members of Aliivibrio (Fig. 2). These ...
It iss somehow related to Aliivibrio fischeri.[citation needed] It is native to the Red Sea, and lives in symbiosis with big- ...
Aliivibrio fischeri ,Broad category or phylum: Proteobacteria. ,Cell shape and structure: gram-negative, rod shaped, ...
Aliivibrio fischeri (strain ATCC 700601 / ES114) (Vibrio fischeri). 344. Q6LN52. METN_PHOPR. ...
Aliivibrio fischeri. all known species names for this strain. Aliivibrio fischeri. Vibrio fischeri. ...
Microencapsulated Aliivibrio fischeri in Alginate Microspheres for Monitoring Heavy Metal Toxicity in Environmental Waters ...
Embedded in the biofilm are Aliivibrio fischeri bacteria (rod-shaped). - Stock Image C026/7515 ... Keywords: aliivibrio fischeri, bacteria, bacterial, bacteriology, bacterium, biofilm, biological, biology, black background, ... Embedded in the biofilm are Aliivibrio fischeri bacteria (rod-shaped).. Release details: Model release not required. Property ...
Characterization of Aliivibrio fischeri strains associated with disease outbreak in brill Scophthalmus rhombus. DAO 124:215-222 ...
Aliivibrio fischeri/physiology*. *Alkaline Phosphatase/genetics/metabolism*. *Decapodiformes/enzymology*/genetics/microbiology/ ... C and D) Localization of V. fischeri producing red fluorescent protein (RFP). Arrows indicate where the AP activity and ... C and D) Localization of V. fischeri producing red fluorescent protein (RFP). Arrows indicate where the AP activity and ... Unlabelled: The synergistic activity of Vibrio fischeri lipid A and the peptidoglycan monomer (tracheal cytotoxin [TCT]) ...
Aliivibrio fischeri, Aliivibrio fischeri ES114, Euprymna scolopes. J. Bacteriol.. 190. 5814-5823 ...
Toxicity Test Kits, Aliivibrio fischeri (formerly Vibrio f.), luminescence, Microbiotests, ATP Test Kits, Algal Toxin Tests. ...
... polar and ionisable chemicals and their mixtures in the bioluminescence inhibition assay with Aliivibrio fischeri. Environ. Sci ...
  • For mono-, di- and tri-chlorophenols multiple linear regression analysis of V. fischeri toxicity showed a good correlation with the solvent accessible surface area and the charge on the oxygen atom. (qub.ac.uk)
  • This correlation successfully predicted the toxicity of the heavily chlorinated phenols, suggesting in V. fischeri only one overall mechanism is present for all chlorophenols. (qub.ac.uk)
  • In this study, the acute toxicity of NTO and ATO towards various organisms, including microorganisms (i.e., methanogenic archaea, aerobic heterotrophs, and Aliivibrio fischeri (Microtox assay)), the microcrustacean Daphnia magna (ATO only), and zebrafish embryos (Danio rerio), was assessed. (oregonstate.edu)
  • Toxicity of individual pharmaceuticals and their mixtures to Aliivibrio fischeri: Experimental results for single compounds and considerations of their mechanisms of action and potential acute effects on aquatic organisms. (semanticscholar.org)
  • The toxicity was determined by tests with Aliivibrio fischeri. (bvsalud.org)
  • The WaterTox STD (Standard) 1243-100 kit is packaged to provide users with a complete setup to replace traditional Vibrio Fischeri acute toxicity tests (Microtox users). (ebpi-kits.com)
  • The WaterTox EVO (Evolution) Vibrio Fischeri kit is packaged as a cost effective alternative to the 1232-1000 Water Toxicity Kit. (ebpi-kits.com)
  • By using quorum sensing to limit the production of luciferase to situations when cell populations are large, V. fischeri cells are able to avoid wasting energy on the production of useless product. (ipfs.io)
  • Transcriptional characterization of Vibrio fischeri during colonization of juvenile Euprymna scolopes. (nih.gov)
  • Integration of these transcriptional data with a recently developed metabolic model of V. fischeri provides us with a clearer picture of the metabolic state of symbionts within the juvenile host, including their possible carbon sources. (nih.gov)
  • Predicted secondary structure [obtained using MFOLD ()] for CsrB1 in V.fischeri showing multiple AGGA/ARGGA sequence motifs in the loop regions. (nih.gov)
  • The LumoPlate Vibrio Fischeri assay accounts for sample coloration and turbidity and can be used for direct measurement of soil and sediment samples without time consuming correction procedures. (ebpi-kits.com)
  • Three of the genomes included in the 44 taxon dataset, those of Vibrio gazogenes , Salinivibrio costicola , and Aliivibrio logei have been newly sequenced and their genome sequences are documented here. (biomedcentral.com)
  • We sought to identify the genes activated by the sigma54-dependent flagellar master regulator, FlrA, in V. fischeri, thereby determining the flagellar regulon in this model symbiont. (datamed.org)
  • In A. fischeri, five such genes (luxCDABEG) have been identified as active in the emission of visible light, and two genes (luxR and luxI) are involved in regulating the operon. (wikipedia.org)
  • Aliivibrio fischeri" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (ouhsc.edu)
  • A. fischeri inhabit photophores in the mantle of the Hawaiian bobtail squid. (wikipedia.org)
  • A. fischeri cells in the ocean inoculate the light organs of juvenile squid and fish. (wikipedia.org)
  • Schematic cartoons of (A) the colonization of E. scolopes by V. fischeri during the study's 3-stage transcriptomic comparison, and (B) characteristic metabolic pathways that are differentially expressed in planktonic and squid-associated cells of V. fischeri . (nih.gov)
  • V. fischeri causes the squid to produce a mucus substance that is secreted below the light organ on the squid. (sciencephoto.com)
  • We report here the first full RNA-Seq dataset representing host-associated V. fischeri cells from colonized juvenile E. scolopes, as well as comparative transcriptomes under both laboratory and simulated marine planktonic conditions. (nih.gov)
  • The overall model can be seen in figures in [http://2012.igem.org/Team:Colombia/Project/Experiments/Aliivibrio_and_Streptomyces the main page of Aliivibrio/Streptomyces. (igem.org)
  • When A. fischeri cells are free-living (or planktonic ), the autoinducer is at low concentration, and, thus, cells do not luminesce. (ipfs.io)
  • The acute ecotoxic effects of ibuprofen and omeprazole and their mixture have been measured using Aliivibrio fischeri as a biomodel. (usda.gov)
  • The population of A. fischeri needs to reach an optimal level to activate the lux operon and stimulate light production. (wikipedia.org)
  • Poorer correlations were found with P. fluorescens highlighting the relative similarity of V. fischeri and RASC c2, in contrast to that of P. fluorescens. (qub.ac.uk)