AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesInformation Science
Aliivibrio fischeriAliivibrio salmonicidaAliivibrioAliivibrio InfectionsVibrionaceaeVibrioDecapodiformesGadiformesSymbiosisAnimal StructuresLuminescent MeasurementsLuminescenceNucleoside-Phosphate KinaseBivalvia4-ButyrolactoneBacterial ProteinsNeosartoryaRec A RecombinasesPhotobacteriumDNA, RibosomalMolecular Sequence DataDNA, BacterialQuorum SensingPhylogenyDNA-Directed RNA PolymerasesGene Expression Regulation, BacterialRNA, Ribosomal, 16S
Aliivibrio fischeri
A species of gram-negative bacteria in the genus ALIIVIBRIO, which exhibits LUMINESCENCE. A. fischeri is found in a symbiotic relationship with the SQUID Euprymna scolopes.
Aliivibrio salmonicida
A species of gram-negative bacteria in the genus ALIIVIBRIO. Aliivibrio salmonicida is the causative agent of cold water vibriosis, a HEMORRHAGIC SEPTICEMIA of ATLANTIC SALMON.
Aliivibrio
A genus of gram-negative, rod-shaped bacteria in the family VIBRIONACEAE. They are found in the marine environment often associated with animals.
Aliivibrio Infections
Infections with bacteria of the genus ALIIVIBRIO.
Vibrionaceae
A family of gram-negative bacteria whose members predominate in the bacterial flora of PLANKTON; FISHES; and SEAWATER. Some members are important pathogens for humans and animals.
Vibrio
A genus of VIBRIONACEAE, made up of short, slightly curved, motile, gram-negative rods. Various species produce cholera and other gastrointestinal disorders as well as abortion in sheep and cattle.
Decapodiformes
A superorder of CEPHALOPODS comprised of squid, cuttlefish, and their relatives. Their distinguishing feature is the modification of their fourth pair of arms into tentacles, resulting in 10 limbs.
Gadiformes
An order of fish including the families Gadidae (cods), Macrouridae (grenadiers), and hakes. The large Gadidae family includes cod, haddock, whiting, and pollock.
Symbiosis
The relationship between two different species of organisms that are interdependent; each gains benefits from the other or a relationship between different species where both of the organisms in question benefit from the presence of the other.
Animal Structures
Organs and other anatomical structures of non-human vertebrate and invertebrate animals.
Luminescent Measurements
Techniques used for determining the values of photometric parameters of light resulting from LUMINESCENCE.
Luminescence
Emission of LIGHT when ELECTRONS return to the electronic ground state from an excited state and lose the energy as PHOTONS. It is sometimes called cool light in contrast to INCANDESCENCE. LUMINESCENT MEASUREMENTS take advantage of this type of light emitted from LUMINESCENT AGENTS.
Nucleoside-Phosphate Kinase
An enzyme that catalyzes reversible reactions of a nucleoside triphosphate, e.g., ATP, with a nucleoside monophosphate, e.g., UMP, to form ADP and UDP. Many nucleoside monophosphates can act as acceptor while many ribo- and deoxyribonucleoside triphosphates can act as donor. EC 2.7.4.4.
Bivalvia
A class in the phylum MOLLUSCA comprised of mussels; clams; OYSTERS; COCKLES; and SCALLOPS. They are characterized by a bilaterally symmetrical hinged shell and a muscular foot used for burrowing and anchoring.
4-Butyrolactone
One of the FURANS with a carbonyl thereby forming a cyclic lactone. It is an endogenous compound made from gamma-aminobutyrate and is the precursor of gamma-hydroxybutyrate. It is also used as a pharmacological agent and solvent.
Bacterial Proteins
Proteins found in any species of bacterium.
Neosartorya
A genus of ascomycetous fungi in the family Trichocomaceae, order EUROTIALES. Some species can cause opportunistic infections in humans, similar to its anamorph ASPERGILLUS.
Rec A Recombinases
A family of recombinases initially identified in BACTERIA. They catalyze the ATP-driven exchange of DNA strands in GENETIC RECOMBINATION. The product of the reaction consists of a duplex and a displaced single-stranded loop, which has the shape of the letter D and is therefore called a D-loop structure.
Photobacterium
A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria that are common in the marine environment and on the surfaces and in the intestinal contents of marine animals. Some species are bioluminescent and are found as symbionts in specialized luminous organs of fish.
DNA, Ribosomal
DNA sequences encoding RIBOSOMAL RNA and the segments of DNA separating the individual ribosomal RNA genes, referred to as RIBOSOMAL SPACER DNA.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
DNA, Bacterial
Deoxyribonucleic acid that makes up the genetic material of bacteria.
Quorum Sensing
A phenomenon where microorganisms communicate and coordinate their behavior by the accumulation of signaling molecules. A reaction occurs when a substance accumulates to a sufficient concentration. This is most commonly seen in bacteria.
Phylogeny
The relationships of groups of organisms as reflected by their genetic makeup.
DNA-Directed RNA Polymerases
Enzymes that catalyze DNA template-directed extension of the 3'-end of an RNA strand one nucleotide at a time. They can initiate a chain de novo. In eukaryotes, three forms of the enzyme have been distinguished on the basis of sensitivity to alpha-amanitin, and the type of RNA synthesized. (From Enzyme Nomenclature, 1992).
Gene Expression Regulation, Bacterial
Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.
RNA, Ribosomal, 16S
Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.

Microbial factor-mediated development in a host-bacterial mutualism. (1/147)

Tracheal cytotoxin (TCT), a fragment of the bacterial surface molecule peptidoglycan (PGN), is the factor responsible for the extensive tissue damage characteristic of whooping cough and gonorrhea infections. Here, we report that Vibrio fischeri also releases TCT, which acts in synergy with lipopolysaccharide (LPS) to trigger tissue development in its mutualistic symbiosis with the squid Euprymna scolopes. As components of PGN and LPS have commonly been linked with pathogenesis in animals, these findings demonstrate that host interpretation of these bacterial signal molecules is context dependent. Therefore, such differences in interpretation can lead to either inflammation and disease or to the establishment of a mutually beneficial animal-microbe association.  (+info)

Complete genome sequence of Vibrio fischeri: a symbiotic bacterium with pathogenic congeners. (2/147)

Vibrio fischeri belongs to the Vibrionaceae, a large family of marine gamma-proteobacteria that includes several dozen species known to engage in a diversity of beneficial or pathogenic interactions with animal tissue. Among the small number of pathogenic Vibrio species that cause human diseases are Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus, the only members of the Vibrionaceae that have had their genome sequences reported. Nonpathogenic members of the genus Vibrio, including a number of beneficial symbionts, make up the majority of the Vibrionaceae, but none of these species has been similarly examined. Here we report the genome sequence of V. fischeri ES114, which enters into a mutualistic symbiosis in the light organ of the bobtail squid, Euprymna scolopes. Analysis of this sequence has revealed surprising parallels with V. cholerae and other pathogens.  (+info)

Magnesium promotes flagellation of Vibrio fischeri. (3/147)

The bacterium Vibrio fischeri requires bacterial motility to initiate colonization of the Hawaiian squid Euprymna scolopes. Once colonized, however, the bacterial population becomes largely unflagellated. To understand environmental influences on V. fischeri motility, we investigated migration of this organism in tryptone-based soft agar media supplemented with different salts. We found that optimal migration required divalent cations and, in particular, Mg2+. At concentrations naturally present in seawater, Mg2+ improved migration without altering the growth rate of the cells. Transmission electron microscopy and Western blot experiments suggested that Mg2+ addition enhanced flagellation, at least in part through an effect on the steady-state levels of flagellin protein.  (+info)

Vibrio fischeri uses two quorum-sensing systems for the regulation of early and late colonization factors. (4/147)

Vibrio fischeri possesses two quorum-sensing systems, ain and lux, using acyl homoserine lactones as signaling molecules. We have demonstrated previously that the ain system activates luminescence gene expression at lower cell densities than those required for lux system activation and that both systems are essential for persistent colonization of the squid host, Euprymna scolopes. Here, we asked whether the relative contributions of the two systems are also important at different colonization stages. Inactivation of ain, but not lux, quorum-sensing genes delayed initiation of the symbiotic relationship. In addition, our data suggest that lux quorum sensing is not fully active in the early stages of colonization, implying that this system is not required until later in the symbiosis. The V. fischeri luxI mutant does not express detectable light levels in symbiosis yet initiates colonization as well as the wild type, suggesting that ain quorum sensing regulates colonization factors other than luminescence. We used a recently developed V. fischeri microarray to identify genes that are controlled by ain quorum sensing and could be responsible for the initiation defect. We found 30 differentially regulated genes, including the repression of a number of motility genes. Consistent with these data, ain quorum-sensing mutants displayed an altered motility behavior in vitro. Taken together, these data suggest that the sequential activation of these two quorum-sensing systems with increasing cell density allows the specific regulation of early colonization factors (e.g., motility) by ain quorum sensing, whereas late colonization factors (e.g., luminescence) are preferentially regulated by lux quorum sensing.  (+info)

Identifying components of the NF-kappaB pathway in the beneficial Euprymna scolopes-Vibrio fischeri light organ symbiosis. (5/147)

The Toll/NF-kappaB pathway is a common, evolutionarily conserved innate immune pathway that modulates the responses of animal cells to microbe-associated molecular patterns (MAMPs). Because MAMPs have been implicated as critical elements in the signaling of symbiont-induced development, an expressed sequence tag library from the juvenile light organ of Euprymna scolopes was used to identify members of the Toll/NF-kappaB pathway. Full-length transcripts were identified by using 5' and 3' RACE PCR. Seven transcripts critical for MAMP-induced triggering of the Toll/NF-kappaB phosphorylation cascade have been identified, including receptors, signal transducers, and a transcription factor. Further investigations should elucidate the role of the Toll/NF-kappaB pathway in the initiation of the beneficial symbiosis between E. scolopes and Vibrio fischeri.  (+info)

The LuxR receptor: the sites of interaction with quorum-sensing signals and inhibitors. (6/147)

The function of LuxR homologues as quorum sensors is mediated by the binding of N-acyl-L-homoserine lactone (AHL) signal molecules to the N-terminal receptor site of the proteins. In this study, site-directed mutagenesis was carried out of the amino acid residues comprising the receptor site of LuxR from Vibrio fischeri, and the ability of the L42A, L42S, Y62F, W66F, D79N, W94D, V109D, V109T and M135A LuxR mutant proteins to activate green fluorescent protein expression from a P(luxI) promoter was measured. X-ray crystallographic studies of the LuxR homologue TraR indicated that residues Y53 and W57 form hydrogen bonds to the 1-carbonyl group and the ring carbonyl group, respectively, of the cognate AHL signal. Based on the activity and signal specificity of the LuxR mutant proteins, and on molecular modelling, a model is suggested in which Y62 (corresponding to Y53 in TraR) forms a hydrogen bond with the ring carbonyl group rather than the 1-carbonyl group, while W66 (corresponding to W57 in TraR) forms a hydrogen bond to the 1-carbonyl group. This flips the position of the acyl side chain in the LuxR/signal molecule complex compared to the TraR/signal molecule complex. Halogenated furanones from the marine alga Delisea pulchra and the synthetic signal analogue N-(sulfanylacetyl)-L-homoserine lactone can block quorum sensing. The LuxR mutant proteins were insensitive to inhibition by N-(propylsulfanylacetyl)-L-homoserine lactone. In contrast, the mutations had only a minor effect on the sensitivity of the proteins to halogenated furanones, and the data strongly suggest that these compounds do not compete in a 'classic' way with N-3-oxohexanoyl-L-homoserine lactone for the binding site. Based on modelling and experimental data it is suggested that these compounds bind in a non-agonist fashion.  (+info)

New rfp- and pES213-derived tools for analyzing symbiotic Vibrio fischeri reveal patterns of infection and lux expression in situ. (7/147)

Genetically altered or tagged Vibrio fischeri strains can be observed in association with their mutualistic host Euprymna scolopes, providing powerful experimental approaches for studying this symbiosis. Two limitations to such in situ analyses are the lack of suitably stable plasmids and the need for a fluorescent tag that can be used in tandem with green fluorescent protein (GFP). Vectors previously used in V. fischeri contain the p15A replication origin; however, we found that this replicon is not stable during growth in the host and is retained by fewer than 20% of symbionts within a day after infection. In contrast, derivatives of V. fischeri plasmid pES213 were retained by approximately 99% of symbionts even 3 days after infection. We therefore constructed pES213-derived shuttle vectors with a variety of selectable and visual markers. To include a visual tag that can be used in conjunction with GFP, we compared seven variants of the DsRed2 red fluorescent protein (RFP): mRFP1, tdimer2(12), DsRed.T3, DsRed.T4, DsRed.M1, DsRed.T3_S4T, and DsRed.T3(DNT). The last variant was brightest, displaying >20-fold more fluorescence than DsRed2 in V. fischeri. RFP expression did not detectably affect the fitness of V. fischeri, and cells were readily visualized in combination with GFP-expressing cells in mixed infections. Interestingly, even when inocula were dense enough that most E. scolopes hatchlings were infected by two strains, there was little mixing of the strains in the light organ crypts. We also used constitutive RFP in combination with the luxICDABEG promoter driving expression of GFP to visualize the spatial and temporal induction of this bioluminescence operon during symbiotic infection. Our results demonstrate the utility of pES213-based vectors and RFP for in situ experimental approaches in studies of the V. fischeri-E. scolopes symbiosis.  (+info)

Cyclo(Phe-Pro) modulates the expression of ompU in Vibrio spp. (8/147)

Vibrio vulnificus was found to produce a chemical that induced the expression of Vibrio fischeri lux genes. Electron spray ionization-mass spectrometry and 1H nuclear magnetic resonance analyses indicated that the compound was cyclo(L-Phe-L-Pro) (cFP). The compound was produced at a maximal level when cell cultures reached the onset of stationary phase. Sodium dodecyl sulfate-polyacrylamide gel analysis of the total proteins of V. vulnificus indicated that expression of OmpU was enhanced by exogenously added synthetic or purified cFP. A toxR-null mutant failed to express ompU despite the addition of cFP. The related Vibrio spp. V. cholerae, V. parahaemolyticus, and V. harveyi also produced cFP, which induced the expression of their own ompU genes. cFP also enhanced the expression in V. cholerae of the ctx genes, which are known to be regulated by ToxR. Our results suggest that cFP is a signal molecule controlling the expression of genes important for the pathogenicity of Vibrio spp.  (+info)

Bacterial symbionts induce host organ morphogenesis during early postembryonic development of the squid Euprymna scolopes |...Bacterial symbionts induce host organ morphogenesis during early postembryonic development of the squid Euprymna scolopes |...
The mutualistic association between the squid Euprymna scolopes and the bacterium Vibrio fischeri is an emerging experimental system for the study of the influence of bacteria on animal development. Taking advantage of the ability to raise both this host and its microbial partner independently under laboratory conditions, we describe the effects of bacterial interactions on morphogenesis of the juvenile host symbiotic organ. Our results show that bacteria are essential for normal postembryonic development of the symbiotic organ, which involves changes in both the surface epithelium and the epithelial tissue within the organ where the bacterial culture will take up residence. Cell death induced by exposure to symbiotic V. fischeri results in the regression of a complex ciliated surface epithelium, a tissue that apparently functions to facilitate inoculation of the juvenile organ with the appropriate specific bacterial species. Regression of this tissue begins within hours of exposure to ...
https://dev.biologists.org/content/120/7/1719?ijkey=82cc26868983f461cce960ba3f8780da80ff45df&keytype2=tf_ipsecsha
Effects of Vibrio fischeri colonization on cognition, foraging behavior, and survival in the Hawaiian bobtail squid
Effects of Vibrio fischeri colonization on cognition, foraging behavior, and survival in the Hawaiian bobtail squid Conference Paper ...
http://vivo.mblwhoilibrary.org/display/publication249530
Domain combinations for D-ribose-5-phosphate isomerase (RpiA), lid domain superfamily in Vibrio fischeri ES114Domain combinations for D-ribose-5-phosphate isomerase (RpiA), lid domain superfamily in Vibrio fischeri ES114
Domain combinations containing the D-ribose-5-phosphate isomerase (RpiA), lid domain superfamily in Vibrio fischeri ES114. Domain architectures illustrate each occurrence of the D-ribose-5-phosphate isomerase (RpiA), lid domain superfamily.
http://supfam.mrc-lmb.cam.ac.uk/SUPERFAMILY/cgi-bin/allcombs.cgi?genome=vf&sf=75445
Domain assignment for gi|59711463|ref|YP 204239.1| from Vibrio fischeri ES114Domain assignment for gi|59711463|ref|YP 204239.1| from Vibrio fischeri ES114
Domain architecture and assignment details (superfamily, family, region, evalue) for gi|59711463|ref|YP_204239.1| from Vibrio fischeri ES114. Plus protein sequence and external database links.
http://supfam.mrc-lmb.cam.ac.uk/SUPERFAMILY/cgi-bin/gene.cgi?genome=vf&seqid=gi%7C59711463%7Cref%7CYP_204239.1%7C
Effect of the Squid Host on the Abundance and Distribution of Symbiotic Vibrio fischeri in Nature | Applied and Environmental...Effect of the Squid Host on the Abundance and Distribution of Symbiotic Vibrio fischeri in Nature | Applied and Environmental...
Euprymna scolopes, a Hawaiian species of bioluminescent squid, harbors Vibrio fischeri as its specific light organ symbiont. The population of symbionts grew inside the adult light organ with an average doubling time of about 5 h, which produced an excess of cells that were expelled into the surrounding seawater on a diurnal basis at the beginning of each period of daylight. These symbionts, when expelled into the ambient seawater, maintain or slightly increase their numbers for at least 24 h. Hence, locations inhabited by their hosts periodically receive a daily input of symbiotic V. fischeri cells and, as a result, become significantly enriched with these bacteria. As estimated by hybridization with a species-specific luxA gene probe, the typical number of V. fischeri CFU, both in the water column and in the sediments of E. scolopes habitats, was as much as 24 to 30 times that in similar locations where squids were not observed. In addition, the number of symbiotic V. fischeri CFU in seawater ...
https://aem.asm.org/content/60/5/1565?ijkey=8fb458687942607639f754146610b9a7f89c36d0&keytype2=tf_ipsecsha
Vibrio fischeri, symbiotic bacterium, SEM - Stock Image C032/1744 - Science Photo LibraryVibrio fischeri, symbiotic bacterium, SEM - Stock Image C032/1744 - Science Photo Library
Coloured scanning electron micrograph (SEM) of Vibrio fischeri, Gram-negative, motile, bioluminescent, curved rod-shaped bacterium (prokaryote). Vibrio fischeri is a bioluminescent bacterium that occurs in global ocean environments. V. fischeri is most often found in a symbiotic relationship with certain deep sea marine life, such as the bobtail squid, within special light-organs.. V. fischeri causes the squid to produce a mucus substance that is secreted below the light organ on the squid. Cilia are used to draw more bacteria into this mucus matrix. The bacteria migrate into the light organ and colonize an area within it. They provide a light source for the squid to make it blend in with the ocean floor at night. Thus predators have a hard time seeing the squid. V. fischeri is a key research organism for examination of microbial bioluminescence, quorum sensing, and bacterial-animal symbiosis. Magnification: x8,000 when shortest axis printed at 25 - Stock Image C032/1744
http://www.sciencephoto.com/media/798563/view
Themes of Parasitology: March 2011
Courtesy of: http://dels-old.nas.edu/oceans/oceans_and_human_health_part_2.shtml Introduction: This blog examines the relationship between the Hawaiian Bobtail Squid and a bioluminescent bacterium. The Hawaiian Bobtail squid acquires an enhanced ability to capture prey and avoid predators, while the bacterium receives key nutrients from residing within the squid [2]. Distribution of this aquatic relationship is limited by the habitat of the squid (Hawaii). Several bioluminescent bacteria cycle through a single squid daily. Description of Relationship: The type of relationship observed between Euprymna scolope(Hawaiian Bobtail squid) and Vibrio fischeri(bioluminescent bacterium) is unique to organisms with specialized structures called light organs. These light organs are specialized structures that have evolved to allow the organisms that house them to create light. While the evolutionary reason for this structure is unknown, many individuals hypothesize that certain marine organisms form these ...
http://bio390parasitology.blogspot.ca/2011/03/
In the Hawaiian bobtail squid (pictured), scientists have found that the same bacterial toxin that causes whooping cough and gon
Description: In the Hawaiian bobtail squid (pictured), scientists have found that the same bacterial toxin that causes whooping cough and gonorrhea in humans is responsible for the development of an important organ. The finding, reported in the Nov. 12 Science magazine, suggests that the role of bacteria in their host organisms depends significantly on the biological context in which they are found. In one place, a bacterium may have a harmful role; in another, it may be critical for the animals survival. The corresponding author of the Science paper is Margaret McFall-Ngai, a UW-Madison professor of medical microbiology ...
http://gallery.xcski.com/index.php/misc-pics/JimHensonsChuthluBabies
Bobtail squid - WikipediaBobtail squid - Wikipedia
Bobtail squid (order Sepiolida) are a group of cephalopods closely related to cuttlefish. Bobtail squid tend to have a rounder mantle than cuttlefish and have no cuttlebone. They have eight suckered arms and two tentacles and are generally quite small (typical male mantle length being between 1 and 8 cm). Sepiolids live in shallow coastal waters of the Pacific Ocean and some parts of the Indian Ocean and Atlantic Ocean as well as in shallow waters on the west coast of the Cape Peninsula off South Africa. Like cuttlefish, they can swim by either using the fins on their mantle or by jet propulsion. They are also known as dumpling squid (owing to their rounded mantle) or stubby squid. Bobtail squid have a symbiotic relationship with bioluminescent bacteria (Aliivibrio fischeri), which inhabit a special light organ in the squids mantle. The bacteria are fed a sugar and amino acid solution by the squid and in return hide the squids silhouette when viewed from below by matching the amount of light ...
https://en.wikipedia.org/wiki/Bobtail_squid
New rfp- and pES213-Derived Tools for Analyzing Symbiotic Vibrio fischeri Reveal Patterns of Infection and lux Expression In...New rfp- and pES213-Derived Tools for Analyzing Symbiotic Vibrio fischeri Reveal Patterns of Infection and lux Expression In...
Genetically altered or tagged Vibrio fischeri strains can be observed in association with their mutualistic host Euprymna scolopes, providing powerful experimental approaches for studying this symbiosis. Two limitations to such in situ analyses are the lack of suitably stable plasmids and the need for a fluorescent tag that can be used in tandem with green fluorescent protein (GFP). Vectors previously used in V. fischeri contain the p15A replication origin; however, we found that this replicon is not stable during growth in the host and is retained by fewer than 20% of symbionts within a day after infection. In contrast, derivatives of V. fischeri plasmid pES213 were retained by ∼99% of symbionts even 3 days after infection. We therefore constructed pES213-derived shuttle vectors with a variety of selectable and visual markers. To include a visual tag that can be used in conjunction with GFP, we compared seven variants of the DsRed2 red fluorescent protein (RFP): mRFP1, tdimer2(12), DsRed.T3, ...
https://aem.asm.org/content/72/1/802.abstract
Comparative genomics-based investigation of resequencing targets in Vibrio fischeri: focus on point miscalls and artefactual...Comparative genomics-based investigation of resequencing targets in Vibrio fischeri: focus on point miscalls and artefactual...
Abstract Background Sequence closure often represents the end-point of a genome project, without a system in place for subsequent improvement and refinement. Building on the genome project of Vibrio fischeri ES114, we used a comparative approach to identify and investigate genes that had a high likelihood of sequence error. Results Comparison of the V. fischeri ES114 genome with that of conspecific strain MJ11 identified 82 target loci in ES114 as containing likely errors, and thus of high-priority for resequencing. Analysis of the targets identified 75 loci in which an error had occurred, resulting in the correction of 10,457 base pairs to generate the new ES114 genomic sequence. A majority of the inaccurate loci involved frameshift errors, correction of which fused adjacent ORFs. Although insertions/deletions are thought to be rare in microbial genome assemblies, fourteen of the loci contained extraneous sequence of over 300 bp, likely due to imperfect contig ends that were misassembled in ...
https://athenaeum.libs.uga.edu/handle/10724/19751
Virulence in Context | Science SignalingVirulence in Context | Science Signaling
Tracheal cytotoxin (TCT) is a bacterial virulence factor in whooping cough (and other infections) that triggers leukocyte infiltration and strips airways of ciliated epithelium. However, not all host-bacterial encounters are negative. In the relationship between the glow-in-the dark squid Euprymna scolopes and its luminescent bacterial symbiont Vibrio fischeri, Koropatnick et al. have discovered that the same toxin plays a benign role. In this association, TCT still initiates apoptosis of ciliated cells and host cell infiltration. However, these cellular responses are triggered when juvenile squid first encounter V. fischeri and constitute a normal developmental stage in the generation of the specialized light organ that allows it to accommodate the incoming symbiont.. T. A. Koropatnick, J. T. Engle, M. A. Apicella, E. V. Stabb, W. E. Goldman, M. J. McFall-Ngai, Microbial factor-mediated development in a host-bacterial mutualism. Science 306, 1186-1188 (2004). [Abstract] [Full Text]. ...
https://stke.sciencemag.org/content/2004/259/tw417
Bioluminescent bacteria - WikipediaBioluminescent bacteria - Wikipedia
Bioluminescent bacteria are light-producing bacteria that are predominantly present in sea water, marine sediments, the surface of decomposing fish and in the gut of marine animals. While not as common, bacterial bioluminescence is also found in terrestrial and freshwater bacteria. These bacteria may be free living (such as Vibrio harveyi) or in symbiosis with animals such as the Hawaiian Bobtail squid (Aliivibrio fischeri) or terrestrial nematodes (Photorhabdus luminescens). The host organisms provide these bacteria a safe home and sufficient nutrition. In exchange, the hosts use the light produced by the bacteria for camouflage, prey and/or mate attraction. Bioluminescent bacteria have evolved symbiotic relationships with other organisms in which both participants benefit close to equally. Another possible reason bacteria use luminescence reaction is for quorum sensing, an ability to regulate gene expression in response to bacterial cell density. Records of bioluminescent bacteria existed for ...
https://en.wikipedia.org/wiki/Bioluminescent_bacteria
A Light in the Darkness | Bugs In Our BackyardA Light in the Darkness | Bugs In Our Backyard
The developmental changes bobtail squid undergo when colonized by the bacteria are both dramatic and accessible for biologists to study. The bobtail squid, Euprymna scolopes, hatches as a fully formed squid. They have no larval stage. However, theres a vestige of the ciliated structures seen in other mollusks. (Cilia are like tiny hairs on the surface of individual cells.) These ciliated cells cover the surface of a pair of appendages in the undeveloped light organ (Koropatnick, 2014). After hatching, the bobtail squid has no association with any bacteria, and is not capable of luminescence. The light organ can only complete its development once it is colonized by a specific species of bacteria, Vibrio fischeri. Once V. fischeri is obtained from the surrounding seawater, the bacteria become suspended in mucus and migrate to the light organ. The cells smell their way! They follow a trail of chemical cues left by the squids cells (Koropatnick, 2014). Approximately 12 hours after hatching, the ...
https://www.bugsinourbackyard.org/a-light-in-the-darkness/
Genomic tRNA Database: Vibrio fischeri ES114Genomic tRNA Database: Vibrio fischeri ES114
The Genomic tRNA Database is curated by Todd Lowe and Patricia Chan. Please email [email protected] with any questions and comments. To submit corrections, please use our Bug and Request Tracking System ...
http://gtrnadb2009.ucsc.edu/Vibr_fisc_ES114/
Aliivibrio fischeri (Beijerinck) Urbanczyk et al. ATCC ® 700601D-5Aliivibrio fischeri (Beijerinck) Urbanczyk et al. ATCC ® 700601D-5
Aliivibrio fischeri ATCC ® 700601D-5™ Designation: Genomic DNA from Aliivibrio fischeri strain ES114 TypeStrain=False Application:
https://www.atcc.org/en/Global/Products/D/F/5/8/700601D-5.aspx
A little squid and its glowing bacteria yield new clues to symbiotic relationshipsA little squid and its glowing bacteria yield new clues to symbiotic relationships
The relationship between the Hawaiian bobtail squid and the bioluminescent bacteria living in its light organ has been studied for decades as a model of symbiosis. Now researchers have used a powerful chemical analysis tool to identify a small molecule produced by the bacteria that appears to play an important role in their colonization of the light organ.. The study, published March 9 in the journal mBio, adds a new wrinkle to scientists understanding of the chemical signaling involved in this iconic symbiotic relationship. Its exciting that there are still new things to discover, even in such a well-studied system, said corresponding author Laura Sanchez, associate professor of chemistry and biochemistry at UC Santa Cruz.. The Hawaiian bobtail squid is a small nocturnal squid, about the size of a thumb, that lives in shallow coastal waters, hiding in the sand during the day and coming out at night to hunt for small shrimp and other prey. The bioluminescent glow from its light organ is ...
https://news.ucsc.edu/2021/03/bioluminescent-squid.html
Amigurumi Bobtail Squid - *pattern included* - CROCHETAmigurumi Bobtail Squid - *pattern included* - CROCHET
Amigurumi Bobtail Squid - *pattern included* - CROCHET - The other day I decided I wanted to make some sort of sea creature, & after a bit of research I decided the Bobtail Squid was the one for me. I wr
https://www.craftster.org/forum/index.php?topic=380268.0
The Color of Blood: Pigment Helps Stage Symbiosis in SquidThe Color of Blood: Pigment Helps Stage Symbiosis in Squid
The relationship between the Hawaiian bobtail squid and the bacterium Vibrio fischeri is well chronicled, but writing in the current issue of the journal Proceedings of the Royal Society B, a group led by University of Wisconsin-Madison microbiologists Margaret McFall-Ngai, Edward Ruby and their colleagues adds a new wrinkle to the story.
http://www.newswise.com/articles/view/617965/?sc=rsla
Complete genome sequence of Vibrio fischeri: A symbiotic bacterium with pathogenic congeners | PNASComplete genome sequence of Vibrio fischeri: A symbiotic bacterium with pathogenic congeners | PNAS
Homologs of Toxin-Encoding Genes. Although V. fischeri is not known to be pathogenic, and strain ES114 is a beneficial symbiont, its genome carries homologs of Vibrio genes that may have toxin activity (Table 3, which is published as supporting information on the PNAS web site). As mentioned earlier, these include two CTX phage-encoded genes, zot (zona occludins toxin) and ace (accessory cholera enterotoxin), the latter of which has been found only in the V. cholerae and V. fischeri genome sequences. The proteins coded for by these genes have been shown to contribute to the structure of CTX phage (17), and their possible roles as toxins remain controversial (31, 32). At this time, it is not known whether the V. fischeri homologs of these two genes are expressed in this species, or whether they might play a role in this bacteriums symbiotic associations.. All sequenced Vibrio species carry genes encoding another putative toxin called RTX (repeats in structural toxins). RTX activity in V. ...
https://www.pnas.org/content/102/8/3004?ijkey=9d02d3e4633c34dbb47a847928342f4533db2341&keytype2=tf_ipsecsha
Toxicity Of Sulcotrione Photoproducts Mixture Towards Vibrio Fischeri In The Aquatic Environment - Archives of Environmental...
PAN Czytelnia Czasopism, Toxicity Of Sulcotrione Photoproducts Mixture Towards Vibrio Fischeri In The Aquatic Environment - Archives of Environmental Protection
http://so.czasopisma.pan.pl/dlibra/publication/99270/edition/85577/content
American Bobtail Cat Breed Profile
The American Bobtail, a relative newcomer on the scene, is increasing in popularity due to its winning combination of wild appearance and friendly demeanour.. Among the few cat breeds that have originated in the United States, the American Bobtail did not receive recognition by the Cat Fanciers Association until February 2000.. American Bobtail History. The first known American Bobtail was Yodie, a stray with a stubby tail who was found by vacationers in the 1960s. Although his parentage is unknown, it was rumoured that he was a hybrid of a bobcat and a domestic cat due to his short tail and wild look. However, this is unlikely, as the males derived from such matings tend to be sterile, whereas Yodie was not. It is more likely that Yodies stumpy tail was the result of a spontaneous mutation.. Yodie was mated at first with a seal point Siamese female and the kittens were born with bobbed tails, which indicated that the bobtail gene was dominant. Yodie and his children were subsequently mated ...
http://www.metaphoricalplatypus.com/articles/animals/cats/cat-breeds/american-bobtail-cat-breed-profile/
Symbiotic bacteria program daily rhythms in squid using light and chemicalsSymbiotic bacteria program daily rhythms in squid using light and chemicals
Glowing bacteria inside squids use light and chemical signals to control circadian-like rhythms in the animals, according to a study to be published in mBio®. The Hawaiian bobtail squid, Euprymna ...
http://www.chemeurope.com/en/news/142422/symbiotic-bacteria-program-daily-rhythms-in-squid-using-light-and-chemicals.html
Christine Terry, PhD - University of LynchburgChristine Terry, PhD - University of Lynchburg
My current research focuses on analyzing DNA sequence determinants of gene expression in the bacteria Vibrio fischeri. Previous studies of the rrn genes in the bacterium Escherichia coli have laid a strong foundation for our understanding of gene expression. These genes have proven to be a successful model for studying transcription in E. coli; therefore I am investigating the rrn genes in other bacterial species to learn more about transcription regulation. Specifically, I use reporter gene constructs to analyze the factors involved in gene expression in V. fischeri. This work will further our understanding of the prokaryotic gene regulation. What we learn about transcription will help us to better understand the process of gene expression in general and how this process is regulated.. ...
https://www.lynchburg.edu/academics/majors-and-minors/biology/faculty-and-staff/christine-terry/
Spontaneous phenotypic suppression of GacA-defective Vibrio fischeri is achieved via mutation of csrA and ihfA | BMC...Spontaneous phenotypic suppression of GacA-defective Vibrio fischeri is achieved via mutation of csrA and ihfA | BMC...
A previously applied alanine-scanning mutagenesis that was performed with the E. coli csrA gene, used to examine the relative contribution each amino acid residue to CsrA protein function [20], provides an invaluable comparator to our spontaneous suppression screen. Our complementary screen differs in that all potential amino acid substitutions are possible, even the single native alanine residue that was not altered in the alanine scan. In our screen, an A36S substitution caused a 20-fold increase in luminescence and restored siderophore activity indicating the highly conserved A36, positioned into the hydrophobic core of the CsrA homodimer, could be critical to protein folding and 3- dimensional stability [20, 23]. Even the conservative substitution of this alanine with the non-polar and fairly compact valine (VCW2G2) altered CsrA function (Fig. 2). Also, only mutations that improve growth would likely improve fitness. Thus, mutations that map to non-conserved regions that do not contribute to ...
https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-015-0509-2
Publications | Mysite
2019. ​. Kim, R.C., Le, D., Ma, K., Heath-Heckman, E.A.C., Whitehorn, N., Kristan, W.B., and Weisblat, D.A. 2019. Behavioral Analysis of Substrate Texture Preference in a Leech, Helobdella austinensis. J. Comp. Physiol A. 205(2):191-202. ​. 2018. ​. Kremer, N., Koch, E., El Filali, A., Zhou, L., Heath-Heckman, E.A.C., Ruby, E., and McFall-Ngai, M. 2018. Persistent interactions with bacterial symbionts directs mature host cell morphology and gene expression in the squid-vibrio symbiosis. mSystems. 3(5).. ​. 2017. ​. Nawroth, J.C., Guo, H., Koch, E., Heath-Heckman, E.A.C., Hermanson, J.C., Ruby, E.G., Dabiri, J.O., Kanso, E., and McFall-Ngai, M. 2017. Motile Cilia Create Fluid-Mechanical Microhabitats for the Active Recruitment of the Host Microbiome. Proc. Natl. Acad. Sci. U.S.A. 114(36): 9510-9516.. ​. Peyer, S.M., Heath-Heckman, E.A.C., and McFall-Ngai, M.J. 2017. Characterization of the Cell Polarity Gene Crumbs During the Early Development and Maintenance of the Squid-Vibrio Light ...
https://www.heathheckmanlab.org/publications
Prediction of CsrA-regulating small RNAs in bacteria and their experimental verification in Vibrio fischeri. - PubMed - NCBIPrediction of CsrA-regulating small RNAs in bacteria and their experimental verification in Vibrio fischeri. - PubMed - NCBI
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
https://www.ncbi.nlm.nih.gov/pubmed/16822857?dopt=Abstract
Vibrio fischeri - WebvisionVibrio fischeri - Webvision
Vision in fishes and crustaceans is a fascinating and understudied area. In past decades, there were far more studies on the visual systems of sea-dwelling creatures, but with the push towards applied or translational research, the number of reports in these species have dropped off, much to our detriment as one never knows where the […]. ...
http://webvision.med.utah.edu/tag/vibrio-fischeri/
Old English Sheepdog (English Bobtail): traits and picturesOld English Sheepdog (English Bobtail): traits and pictures
Old English Sheepdog (English Bobtail): learn more about this animal; its physical traits, temperament, behavior, etc. The Old English Sheepdog, also known as the English Bobtail, is a...
https://www.animalwised.com/dog-breeds/old-english-sheepdog-english-bobtail.html
Bobtails Rabbit and Guinea pig Rescue
Comment: We have now had ginny and cloud live with eddie and enzo for over a month. We have renamed cloud to belle. Ginny is a little bossy with enzo but they seem to be getting along better, they love their food. Eddie and belle are so sweet together and we call them the love birds. Eddie seems protective of little belle who is half his size. The two girls are gorgeous and have given our boys a new lease of life, neutering them and adopting from bobtails was the best decision. Just watching belle follow and copy eddie popcorning is the cutest thing, love hearing them talking again!! Thank you bobtails you do a fantastic job!!! Eddie and enzo say thanks for their new friends xxx ...
http://bobtailsrescue.org.uk/guestbook.asp?Page=18
SearchSearch
This activity explores the content presented in the animated video Natures Cutest Symbiosis: The Bobtail Squid, which describes how the light produced by bacteria living inside bobtail squid helps the squid avoid its predators.. ...
https://www.biointeractive.org/search?sort_by=created&redirect=1&field_biointeractive_topics%5B0%5D=26663&field_biointeractive_topics%5B1%5D=26673&field_bio_curriculum_field_standard_term%5B2%5D=26889&f%5B0%5D=topics%3A29&f%5B1%5D=topics%3A37&f%5B2%5D=topics%3A42&f%5B3%5D=topics%3A60&f%5B4%5D=topics%3A84
How do Bobtail Squid choose their glowing bacterial partner? | ExperimentHow do Bobtail Squid choose their glowing bacterial partner? | Experiment
Experiment is an online platform for funding and sharing scientific discoveries. Push the boundaries of knowledge in biology, chemistry, medicine, physics, computer science, paleontology, economics, engineering, neuroscience, and more.
https://experiment.com/projects/how-do-bobtail-squid-choose-their-glowing-bacterial-partner?s=discover
Dan Wesson Bobtail CCO 45 ACP, Black Ceramic Coat, Night Sights - Impact GunsDan Wesson Bobtail CCO 45 ACP, Black Ceramic Coat, Night Sights - Impact Guns
Dan Wesson Bobtail CCO 45 ACP, Black Ceramic Coat, Night Sights. Dan Wesson 01962. Due to the popularity of the Guardians concealable profile and Commander-length slide, Dan Wesson took it a step further with the CCO - the Concealed Carry Officer - a Commander slide on an Officer frame. With the...
https://www.impactguns.com/Semi-Auto-Handguns/Dan-Wesson-Bobtail-CCO-45ACP-Black-Ceramic-Coat-Night-Sights-806703019628-01962/
Extending a Bobtail to five elementsExtending a Bobtail to five elements
on my 3 element 40 meter bobtail fed at the top of the center element, it made a huge difference how it was wired... I had to connect the center conductor of the coax to the vertical elemennt and the shield to the horizontal phasing wire. It allowed me to work(and conform) my last 3 zones on 40 ...
http://www.eham.net/ehamforum/smf/index.php?topic=59303.0
1956 Cooper Bobtail Sports Racing T39 Spec Sheet1956 Cooper Bobtail Sports Racing T39 Spec Sheet
Description. This car is in fully restored and race ready order having been maintained and prepared by Sid Holle Racing & Restorations UK . Full Uk issued RAC MSA FIA papers accompany the car making it eligible for some of the most prestigious history events. Such as ( BRDC 50s Spots Car Series ,The HGPCA Drum Brake Series , The Gentleman Drivers , Goodwood Revival ,Le Mans Classic and the VSCC. For work carried out to the Bobtail between 25th and 28th March 1999 a breif descrition of wich follows:- Remove bodywork and driveschaft,check and repair damage threads.Refit schaft& checkgear selection.Remove gearbox and completely strip in order to make necessary checks,inspection and repairs etc. Supplied and fitted new 3rd Gear. Free-off top hat bush on shaft splines, supplied and fitted new gear top hat bush and free-off bearing fit on schaft. Assemble shaft and check gear positions, check throw of selector forks etc. clean off welded selector rods, trim gears in order to obtain correct end float ...
http://www.race-cars.com/carsold/cooper/1147803564/1147803564ss.htm
Bobtails Rabbit and Guinea pig Rescue
This wonderful DVD was made as a guide for new rabbit owners, (and some existing owners that need advice). Mostly filmed here at Bobtails Rescue Centre, this DVD offers guidance on subjects such as ...
http://bobtailsrescue.org.uk/news2.asp?newsID=71
Breeds Cats American Bobtail - PetPremiumBreeds Cats American Bobtail - PetPremium
During the 1960s, the American bobtail cat originated in the United States when a female seal point Siamese cat bred with Yodi, an male brown tabby that had an …
https://www.petpremium.com/breeds/cats/american-bobtail/
Decoding Microbial Chatter: Cell-Cell Communication in Bacteria | Journal of BacteriologyDecoding Microbial Chatter: Cell-Cell Communication in Bacteria | Journal of Bacteriology
FIG. 4. Diffusible signaling in bacteria-host associations. (A) Not only do bacteria recognize their own secreted signals (gray circles) to coordinate a response (black diamonds), but eukaryotic cells in the vicinity may also recognize these signals and produce a response. Some of the potential interactions are diagrammed. Various host cells may alter cytokine production (open triangles), mucus production (shaded gray), or other developmental events, such as apoptosis, in response to either the signal itself or the bacterial product of the signal transduction pathway. Hosts may also induce or constitutively synthesize signal-degrading enzymes. (B) Symbiotic Euprymna scolopes-V. fischeri interaction during light organ colonization. LuxA activity is dependent on AHL quorum sensing. The locations at which bacterial cell-cell signaling likely occurs are labeled as 1 and 2. Region 1 is the site of the initial attachment, or aggregation, by bacteria on the surface of the light organ. Region 2 is the ...
https://jb.asm.org/content/187/16/5507/figures-only
Pravachol pill costPravachol pill cost
Numerical values found at purchase pravachol S4 pravachol 4 0mg tablet Data. Proc Natl Acad Sci U S A. Albertin CB, Simakov O, Mitros T, Wang ZY, Pungor JR, Edsinger-Gonzales E, et al. A had a significantly earlier swelling of the ArfA ribosome rescue factor.. BPI proteins and their relatives: Conservation over purchase pravachol evolution and roles in mutualism. The chemistry of negotiation: Rhythmic, glycan-driven acidification in a beneficial symbiosis. RIG-I indicates that symbiont SsrA and acts as a crucial trigger for downstream signaling cascades in the host cells.. Gabriela Aguirre and Susannah Lawhorn purchase pravachol contributed valuable technical help. A transient exposure to symbiosis-competent bacteria induces light organ by Vibrio fischeri. A) Localization of the expression of ssrA and smpB transcripts by weblink cells of WT V. To build pSMG3, we amplified two fragments: PCRa, approximately 900 bp upstream of smpB; and PCRb, approximately 800 bp downstream of smpB (S1 ...
http://www.lesavenieres.fr/pravachol-pill-cost/
GephebaseGephebase
Most mammals possess a tail, humans and the Great Apes being notable exceptions. One approach to understanding the mechanisms and evolutionary forces influencing development of a tail is to identify the genetic factors that influence extreme tail length variation within a species. In mice, the Tailless locus has proven to be complex, with evidence of multiple different genes and mutations with pleiotropic effects on tail length, fertility, embryogenesis, male transmission ratio, and meiotic recombination. Five cat breeds have abnormal tail length phenotypes: the American Bobtail, the Manx, the Pixie-Bob, the Kurilian Bobtail, and the Japanese Bobtail. We sequenced the T gene in several independent lineages of Manx cats from both the US and the Isle of Man and identified three 1-bp deletions and one duplication/deletion, each predicted to cause a frameshift that leads to premature termination and truncation of the carboxy terminal end of the Brachyury protein. Ninety-five percent of Manx cats ...
https://www.gephebase.org/view-gephe/2305
Euprymna schneehageniEuprymna schneehageni
Members of the class Cephalopoda are gonochoric. Male and female adults usually die shortly after spawning and brooding, respectively. Mating behavior: Males perform various displays to attract potential females for copulation. During copulation, male grasp the female and inserts the hectocotylus into the females mantle cavity where fertilization usually occurs. Life cycle: Embryos hatch into planktonic stage and live for some time before they grow larger and take up a benthic existence as adults (Ref. 833). ...
http://sealifebase.org/summary/Euprymna-schneehageni.html
All hail! Bacteria that control their squid overlords | Deep Sea NewsAll hail! Bacteria that control their squid overlords | Deep Sea News
Squid typically arent my thing, but I can certainly be wooed by their microbes. Example: the very awesome symbiotic relationship between the Hawaiian bobtail
http://www.deepseanews.com/2013/06/all-hail-bacteria-that-control-their-squid-overlords/comment-page-1/
Simple & elegant solution to a big problem in DNA based microbial diversity studies - microBEnet: the microbiology of the Built...Simple & elegant solution to a big problem in DNA based microbial diversity studies - microBEnet: the microbiology of the Built...
Many recent studies rely on 16S rRNA-based sequencing approaches to analyze bacterial or archaeal communities found in soil and other environmental samples. While this approach is valuable for determining the relative abundances of different microbial taxa found in a given sample, it does not provide information on how the total abundances of targeted microbes differ across samples. Here we demonstrate how the simple addition of an internal standard at the DNA extraction step allows for the quantitative comparison of taxon abundances across samples. The reliability of this method was assessed in two ways. First, we spiked a dilution series of two different soils with internal standards to ascertain whether we could accurately quantify differences in cell abundances. We tested two different internal standards, adding DNA from Aliivibrio fischeri or Thermus thermophilus, bacterial taxa unlikely to be found in soil. Both standards allowed us to accurately quantify microbial abundances in soil as ...
https://www.microbe.net/2015/08/26/simple-elegant-solution-to-a-big-problem-in-dna-based-microbial-diversity-studies/
Schipperke | AntageneSchipperke | Antagene
Presence of a naturally shortened tail. Description: DNA test can identifying dogs carrying the phenotype Natural Bobtail. Symptoms : Shortened tail, variability according to individuals: missing tail to 3/4 of tail length. Age of onset : _. Frequency : _. Gene involved : T. Tested mutation : c.295C>G. Transmission : Autosomal Dominant. Patent : _. Notes : _. PrintSend to a friendTweet WidgetPartager sur Facebook ...
http://www.antagene.com/en/chien/schipperke
Diffusion of autoinducer is involved in regulation of the Vibrio fischeri luminescence system. | Journal of BacteriologyDiffusion of autoinducer is involved in regulation of the Vibrio fischeri luminescence system. | Journal of Bacteriology
The enzymes for luminescence in Vibrio fischeri are induced by the accumulation of a species-specific metabolite (autoinducer) in the culture medium. Tritium-labeled autoinducer was used to study the mechanism of autoinduction. When 3H-autoinducer was added to suspensions of V. fischeri or Escherichia coli, cellular concentrations equaled external concentrations. For V. fischeri, equilibration of 3H-autoinducer was rapid (within 20 s), and greater than 90% of the cellular tritium remained in unmodified autoinducer. When V. fischeri or E. coli cells containing 3H-autoinducer were transferred to autoinducer-free buffer, 85 to 99.5% of the radiotracer escaped from the cells, depending on the strain. Concentrations of autoinducer as low as 10 nM, which is equivalent to 1 or 2 molecules per cell, were sufficient for induction, and the maximal response to autoinducer occurred at about 200 nM. If external autoinducer concentrations were decreased to below 10 nM after induction had commenced, the ...
https://jb.asm.org/content/163/3/1210?ijkey=64a241bda4dd47e5a1442dd46241a034ff5d7187&keytype2=tf_ipsecsha
Vibrio fischeri Epoxyqueuosine Reductase, Cobalamine-Stimulated, Queosine Biosynthesis (QUEG) Protein (His tag), Recombinant |...Vibrio fischeri Epoxyqueuosine Reductase, Cobalamine-Stimulated, Queosine Biosynthesis (QUEG) Protein (His tag), Recombinant |...
Recombinant Epoxyqueuosine Reductase, Cobalamine-Stimulated, Queosine Biosynthesis (QUEG) Protein (His tag). Species: Vibrio fischeri. Source: Yeast. Order product ABIN1630401.
https://www.antibodies-online.com/protein/1630401/Epoxyqueuosine+Reductase,+Cobalamine-Stimulated,+Queosine+Biosynthesis+QUEG+AA+1-371+protein+His+tag/
ureA - Urease subunit gamma - Aliivibrio fischeri (strain MJ11) - ureA gene & proteinureA - Urease subunit gamma - Aliivibrio fischeri (strain MJ11) - ureA gene & protein
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
https://www.uniprot.org/uniprot/B5FBC8
Characterization of a recombinant aryl β-glucosidase from Neosartorya fischeri NRRL181<...Characterization of a recombinant aryl β-glucosidase from Neosartorya fischeri NRRL181<...
TY - JOUR. T1 - Characterization of a recombinant aryl β-glucosidase from Neosartorya fischeri NRRL181. AU - Kalyani, Dayanand. AU - Lee, Kyoung-Mi. AU - Tiwari, Manish Kumar. AU - Ramachandran, Priyadharshini. AU - Kim, Hoon. AU - Kim, In-Won. AU - Jeya, Marimuthu. AU - Lee, Jung-Kul. PY - 2012. Y1 - 2012. N2 - An isolated gene from Neosartorya fischeri NRRL181 encoding a β-glucosidase (BGL) was cloned, and its nucleotide sequence was determined. DNA sequence analysis revealed an open reading frame of 1,467 bp, capable of encoding a polypeptide of 488 amino acid residues. The gene was over-expressed in Escherichia coli, and the protein was purified using nickel-nitrilotriacetic acid chromatography. The purified recombinant BGL showed a high level of catalytic activity, with V max of 886 μmol min−1 mg-protein−1 and a K m of 68 mM for p-nitrophenyl-β-d-glucopyranoside (pNPG). The optimal temperature for enzyme activity was about 40°C, and the optimal pH was about 6.0. A homology model of ...
https://orbit.dtu.dk/en/publications/characterization-of-a-recombinant-aryl-%CE%B2-glucosidase-from-neosart
The Squid, the Vibrio and the Moon by Ailsa Wild, Aviva Reed, Briony Barr, Gregory Crocetti and Linda Blackall. - Educate...The Squid, the Vibrio and the Moon by Ailsa Wild, Aviva Reed, Briony Barr, Gregory Crocetti and Linda Blackall. - Educate...
The squid sucks in water and the current whooshes Ali and Mai closer and closer Meet Ali and Mai , Vibrio fischeri Bacterium, who live inside Sepio - a Bobtail Squid. These three creatures need each other and live in Symbiosis, helping each other to grow and survive. This story has been broken up into…
https://educateempower.blog/2019/01/18/the-squid-the-vibrio-and-the-moon-by-ailsa-wild-aviva-reed-briony-barr-gregory-crocetti-and-linda-blackall/?like_comment=27728&_wpnonce=450df08a4a
RSCS | definition of RSCS by Medical dictionaryRSCS | definition of RSCS by Medical dictionary
Looking for online definition of RSCS in the Medical Dictionary? RSCS explanation free. What is RSCS? Meaning of RSCS medical term. What does RSCS mean?
http://medical-dictionary.thefreedictionary.com/RSCS
Dead Coral/Bioluminescent Bacteria | Exploring The InvisibleDead Coral/Bioluminescent Bacteria | Exploring The Invisible
Why would someone want to add a fragment of dead coral to a liquid culture of bioluminescent bacteria. I thought why not. This is what happened.
https://exploringtheinvisible.com/2013/10/03/dead-coralbioluminescent-bacteria/
Biosynthetic gene clusters, secondary metabolite profiles, and cards of virulence in the closest nonpathogenic relatives of...
Aspergillus fumigatus is a major human pathogen that causes hundreds of thousands of infections yearly with high mortality rates. In contrast, Aspergillus fischeri and the recently described Aspergillus oerlinghausenensis, the two species most closely related to A. fumigatus, are not known to be pathogenic. Some of the cards of virulence that A. fumigatus possesses are secondary metabolites that impair the host immune system, protect from host immune cell attacks, or acquire key nutrients. Secondary metabolites and the biosynthetic gene clusters (BGCs) that typically encode them often vary within and between fungal species. To gain insight into whether secondary metabolism-associated cards of virulence vary between A. fumigatus, A. oerlinghausenensis, and A. fischeri, we conducted extensive genomic and secondary metabolite profiling analyses. By analyzing multiple A. fumigatus, one A. oerlinghausenensis, and multiple A. fischeri strains, we identified both conserved and diverged secondary ...
https://www.meta.org/papers/evolution-arrests-invasions-of-cooperative/990033902
mns1B - Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1B precursor - Neosartorya fischeri (strain ATCC 1020 / DSM...mns1B - Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1B precursor - Neosartorya fischeri (strain ATCC 1020 / DSM...
Involved in the maturation of Asn-linked oligosaccharides. Progressively trims alpha-1,2-linked mannose residues from Man(9)GlcNAc(2) to produce Man(5)GlcNAc(2) (By similarity).
http://www.uniprot.org/uniprot/A1D1W1
Bioluminescent Bacteria Kill Rivals and Establish Symbiosis in Squid Using Genetic Regulatory FactorsBioluminescent Bacteria Kill Rivals and Establish Symbiosis in Squid Using Genetic Regulatory Factors
The type VI system is found within many different bacteria, said Kirsten R. Guckes, a postdoctoral researcher at Penn State and first author of the paper. It was originally thought to primarily contribute to the virulence of pathogenic bacteria. For example, Vibrio cholerae, the bacteria that causes cholera, uses it. But, we now know that beneficial bacteria, like V. fischeri, also use T6SS to kill other bacteria. Because T6SS is thought to be energetically expensive for the bacteria to produce, and doing so could interfere with the bacterias ability to thrive and produce bioluminescence, understanding how the components of the system are regulated will help us to explain the host-symbiont relationship and the factors that contribute to establishing symbiosis.. A key structural component of T6SS is Hcp, coded for by two functionally redundant genes. The research team showed that the expression of Hcp is dependent on two factors: the alternative sigma factor σ54 and the bacterial enhancer ...
https://scitechdaily.com/bioluminescent-bacteria-kill-rivals-and-establish-symbiosis-in-squid-using-genetic-regulatory-factors/
American Bobtail Cats Available for Adoption in OklahomaAmerican Bobtail Cats Available for Adoption in Oklahoma
Find American Bobtail cats and kittens in oklahoma available for sale and adoption. Its also free to list any cats you have in our classifieds.
https://www.catsnow.com/americanbobtailoklahoma.htm
Bobtail Lizard
The Bobtail Lizard is often seen sun basking on the road - please try not to run them over as they bond with their mate for life and raise their young together.
http://waverock.com.au/fauna-item/bobtail-lizard
Host-derived amino acids support the proliferation of symbiotic bacteria | PNASHost-derived amino acids support the proliferation of symbiotic bacteria | PNAS
Transposon mutants of the prototrophic V. fischeri strain ESR1 (19) were obtained by conjugative transposon mutagenesis with Escherichia coli strain S17-1 λpir (pUT mini-Tn5 Cm) (21), and selection for transconjugants was performed on a Luria broth and NaCl (LBS) agar medium (22) containing (per liter) 100 mg rifampicin, and 5 mg chloramphenicol. This procedure has been shown to cause single transposon insertions that are stable in V. fischeri (23). Amino acid auxotrophs were isolated from this pool of transposon mutants by using a streptozotocin enrichment method (24). Strains that could be complemented by the addition of a single amino acid were identified (25), and nine mutants that were auxotrophic for different amino acids were analyzed further. The growth rate and luminescence of these mutants were indistinguishable from that of strain ESR1 in SWT broth, a complex tryptone-based seawater medium (13); however, each mutant was unable to grow in the minimal-salts medium MSR, a derivative of ...
https://www.pnas.org/content/95/4/1818?ijkey=b2b6d1f492b02b38a9b9f3285cee77275401acaa&keytype2=tf_ipsecsha
Bacteria produce aphrodisiac that sets off protozoan mating swarm - Hail Science
A chemical released by the bacterium, Vibrio fischeri, sends this protozoan, Salpinogoeca rosetta, into a mating swarm, demonstrating that bacteria can dri...
https://hailscience.com/2016/12/05/bacteria-produce-aphrodisiac-that-sets-off-protozoan-mating-swarm/
ISO 11348-2:2007 - Eesti Standardimis- ja AkrediteerimiskeskusISO 11348-2:2007 - Eesti Standardimis- ja Akrediteerimiskeskus
Water quality -- Determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (Luminescent bacteria test) -- Part 2: Method using liquid-dried bacteria
https://www.evs.ee/et/iso-11348-2-2007
Journal: Chemosphere / Publication Year: 2017 / Source: 2017 - PubAg Search ResultsJournal: Chemosphere / Publication Year: 2017 / Source: 2017 - PubAg Search Results
Five sediment cores (S1−S5) were collected from the Shima River to determine the bioavailability of trace metals (Cd, Cr, Cu, Ni, and Zn) using the modified European Community Bureau of Reference (BCR) procedure. The toxic effects of polluted sediment were assessed using the LUMISTox® bioassay with Vibrio fischeri and chemical models such as the toxicity unit (TU) of each metal, sum of all TUs (∑T ...
https://pubag.nal.usda.gov/?f%5Bjournal_name%5D%5B%5D=Chemosphere&f%5Bpublication_year_rev%5D%5B%5D=7983-2017&f%5Bsource%5D%5B%5D=2017&page=3
Reproduction Summary - Pseudoraja fischeriReproduction Summary - Pseudoraja fischeri
Publish in our journal partners Acta Ichthyologica et Piscatoria (0.708, 2017-2018 impact factor) and Journal of Fish Biology (2.038, 2019 impact factor) the results of your primary research on fish growth, weight-length relationships, reproduction (maturity, fecundity, spawning), food and diet composition, introductions and range extensions for faster subsequent entry in FishBase.. ...
https://www.fishbase.org/Reproduction/FishReproSummary.php?ID=51846&GenusName=Pseudoraja&SpeciesName=fischeri&fc=685&StockCode=43282
March | 2018 | mirna array
Fifty nanograms of cDNA was the template for the RT-PCR, with primer concentrations of 250 μM. 2× SYBR Green master mix (Applied Biosystems) and H2O were added to a final reaction volume of 50 μL per well in a MicroAmp Optical 96-well reaction plate (Applied Biosystems). RG7204 purchase Thermal cycler settings were programmed for 52 °C for 2 min, 95 °C for 10 min, then 45 cycles of the following: 95 °C for 15 s, 51 °C for 15 s, and 60 °C for 1 min, which was the data collection point. Ideally, a csrA partial deletion strain would have been used for experiments as has been possible. in other systems (Liu et al., 1995; Lenz et al., 2005). However, repeated attempts failed to generate the desired construct. Therefore, an alternative strategy was employed to modulate CsrA levels whereby either csrA (pJW3) or csrB1 (pJW4) was overexpressed from a stable plasmid construct in two V. fischeri strains, ES114 (wild type) and PMF8 (ΔlitR). This approach was followed, because in factorial design ...
https://mirnaarray.com/2018/03
Two Component Signal Transduction Systems - Free LivingTwo Component Signal Transduction Systems - Free Living
Many bacteria, including V. fischeri, recognize and respond to their environments using two-component regulatory systems (Fig. 2A, reviewed in Stock et al.
https://www.climate-policy-watcher.org/free-living/twocomponent-signal-transduction-systems.html
Effect of CIAP treatment of lipid A on its ability to i | Open-iEffect of CIAP treatment of lipid A on its ability to i | Open-i
Effect of CIAP treatment of lipid A on its ability to induce juvenile light organ apoptosis. (A) Fluorescence micrographs of light organs that had been incubate
https://openi.nlm.nih.gov/detailedresult.php?img=PMC3569863_mbo0021212690009&req=4
Aliivibrio fischeriAliivibrio fischeri
Vibrio salmonicida and Vibrio wodanis as Aliivibrio fischeri gen. nov., comb. nov., Aliivibrio logei comb. nov., Aliivibrio ... Aliivibrio fischeri (also called Vibrio fischeri) is a Gram-negative, rod-shaped bacterium found globally in marine ... In 2014, Hawaiʻian State Senator Glenn Wakai submitted SB3124 proposing Aliivibrio fischeri as the state microbe of Hawaiʻi. ... The genome for A. fischeri also carries mobile genetic elements. A. fischeri are globally distributed in temperate and ...
https://en.wikipedia.org/wiki/Aliivibrio_fischeri
AliivibrioAliivibrio
Vibrio salmonicida and Vibrio wodanis as Aliivibrio fischeri gen. nov., comb. nov., Aliivibrio logei comb. nov., Aliivibrio ... Aliivibrio is a genus in the phylum Pseudomonadota (Bacteria). The name Aliivibrio derives from: Latin alius, other, another, ... Aliivibrio entry in LPSN; Euzéby, J.P. (1997). "List of Bacterial Names with Standing in Nomenclature: a folder available on ... and Aliivibrio wodanis comb. nov". International Journal of Systematic and Evolutionary Microbiology. 57 (12): 2823-2829. doi: ...
https://en.wikipedia.org/wiki/Aliivibrio
State microbeState microbe
Aliivibrio fischeri was selected because it lives in a symbiotic relationship with the native Hawaiʻian bobtail squid, in which ... In February 2014, Senate Bill 3124 was introduced by Senator Glenn Wakai; the bill designates Aliivibrio fischeri as the State ... Aliivibrio fischeri can be found elsewhere. The combined Hawaiʻian Legislature could not agree on which microbe better suited ... and later contested in 2014 by Senator Glenn Wakai's SB3124 bill proposing Aliivibrio fischeri instead. As of December 2017[ ...
https://en.wikipedia.org/wiki/State_microbe
Marine microbiomeMarine microbiome
It lives in a mutualistic symbiosis with the bioluminescent bacteria Aliivibrio fischeri. The bacteria are fed a solution of ... The relationship between the Hawaiian bobtail squid and the bioluminescent bacterium Aliivibrio fischeri is one of the best ...
https://en.wikipedia.org/wiki/Marine_microbiome
List of model organismsList of model organisms
Aliivibrio fischeri, quorum sensing, bioluminescence and animal-bacterial symbiosis with Hawaiian bobtail squid. Bacteroides ...
https://en.wikipedia.org/wiki/List_of_model_organisms
Counter-illuminationCounter-illumination
The light may be produced by the animals themselves, or by symbiotic bacteria, often Aliivibrio fischeri. Counter-illumination ... The luminescent bacterium is often Aliivibrio fischeri, as for example in the Hawaiian bobtail squid. Reducing the silhouette ...
https://en.wikipedia.org/wiki/Counter-illumination
BioluminescenceBioluminescence
They used the bioluminescent bacterium Aliivibrio fischeri, but the maximum lifetime of their product was three days. In April ... "The first engagement of partners in the Euprymna scolopes-Vibrio fischeri symbiosis is a two-step process initiated by a few ...
https://en.wikipedia.org/wiki/Bioluminescence
VibrionaceaeVibrionaceae
Vibrio salmonicida and Vibrio wodanis as Aliivibrio fischeri gen. nov., comb. nov., Aliivibrio logei comb. nov., Aliivibrio ... and Aliivibrio wodanis comb. nov". International Journal of Systematic and Evolutionary Microbiology. 57 (12): 2823-2829. doi: ... ISBN 0-13-144329-1. Urbanczyk H, Ast J, Higgins M, Carson J, Dunlap P (2007). "Reclassification of Vibrio fischeri, Vibrio ... The family Vibrionaceae currently comprises eight validly published genera: Aliivibrio, Catenococcus, Enterovibrio, Grimontia, ...
https://en.wikipedia.org/wiki/Vibrionaceae
Bioluminescent bacteriaBioluminescent bacteria
For example, Aliivibrio fischeri grows favourably in environments that have temperatures between 5 and 30 °C and a pH that is ... Unlike Aliivibrio fischeri, V. harveyi do not possess the luxI/luxR regulatory genes and therefore have a different mechanism ... A well known example of quorum sensing is that which occurs between Aliivibrio fischeri and its host. This process is regulated ... In bioluminescent bacteria, the reclassification of the members ofVibrio fischeri species group as a new genus, Aliivibrio, has ...
https://en.wikipedia.org/wiki/Bioluminescent_bacteria
Euprymna scolopesEuprymna scolopes
"Aliivibrio fischeri lipopolysaccharide induces developmental apoptosis, but not complete morphogenesis, of the Euprymna ... "Symbiotic role of the viable but nonculturable state of Aliivibrio fischeri in Hawaiian coastal seawater" (PDF). Archived from ... "Alterations in the protoeme of the Euprymna scolopes light organ in response to symbiotic Aliivibrio fischeri" (PDF). Archived ... "Isolation and characterization of a visibly luminous variant of Aliivibrio fischeri strain ES114 form the sepiolid squid ...
https://en.wikipedia.org/wiki/Euprymna_scolopes
Glenn WakaiGlenn Wakai
Wakai proposed SB3124 which attempted to establish Aliivibrio fischeri as the state microbe of Hawaiʻi. This was opposing state ...
https://en.wikipedia.org/wiki/Glenn_Wakai
Quorum sensingQuorum sensing
The YenR and YenI proteins produced by the gammaproteobacterium Yersinia enterocolitica are similar to Aliivibrio fischeri LuxR ... When A. fischeri cells are free-living (or planktonic), the autoinducer is at low concentration, and, thus, cells do not show ... In A. fischeri bioluminescence is regulated by AHLs (N-acyl-homoserine lactones) which is a product of the LuxI gene whose ... The bioluminescent bacterium A. fischeri is the first organism in which QS was observed. It lives as a mutualistic symbiont in ...
https://en.wikipedia.org/wiki/Quorum_sensing
Flavobacterium akiainvivensFlavobacterium akiainvivens
... was deferred for a year when it encountered competition from Senator Glenn Wakai's SB3124 proposing Aliivibrio fischeri. In ...
https://en.wikipedia.org/wiki/Flavobacterium_akiainvivens
Bobtail squidBobtail squid
Aliivibrio fischeri), which inhabit a special light organ in the squid's mantle. The luminescent properties of the bacteria ... Symbiosis with A. fischeri from the surrounding seawater is initiated immediately upon hatching, and the bacteria's ...
https://en.wikipedia.org/wiki/Bobtail_squid
CamouflageCamouflage
The reflectin gene is thought to have originated through transposition from symbiotic Aliivibrio fischeri bacteria, which ... fischeri, with divergence occurred through subsequent gene duplication (such as in the case of Sepia officinalis) or gene loss ...
https://en.wikipedia.org/wiki/Camouflage
James TokiokaJames Tokioka
... was deferred for a year when it encountered competition from Senator Glenn Wakai's SB3124 proposing Aliivibrio fischeri. In ...
https://en.wikipedia.org/wiki/James_Tokioka
VibrioVibrio
Aliivibrio fischeri (or V. fischeri) is known for its mutualistic symbiosis with the Hawaiian bobtail squid, which is dependent ... Pollack-Berti A, Wollenberg MS, Ruby EG (2010). "Natural transformation of Vibrio fischeri requires tfoX and tfoY". Environ. ... Norsworthy AN, Visick KL (November 2013). "Gimme shelter: how Vibrio fischeri successfully navigates an animal's multiple ... fischeri, have tufts of polar flagella with sheath (lophotrichous). Typical bacterial flagellum structure contains three ...
https://en.wikipedia.org/wiki/Vibrio
Cell signalingCell signaling
This signaling between cells was first observed in the marine bacterium Aliivibrio fischeri, which produces light when the ...
https://en.wikipedia.org/wiki/Cell_signaling
Marine prokaryotesMarine prokaryotes
The Hawaiian bobtail squid lives in symbiosis with the bioluminescent bacteria Aliivibrio fischeri which inhabits a special ... Aliivibrio fischeri) or terrestrial nematodes (Photorhabdus luminescens). The host organisms provide these bacteria a safe home ... Insights from the Euprymna scolopes-Vibrio fischeri symbiosis". Seminars in Immunology. 24 (1): 3-8. doi:10.1016/j.smim.2011.11 ...
https://en.wikipedia.org/wiki/Marine_prokaryotes
MicrobiomeMicrobiome
The relationship between the Hawaiian bobtail squid and the bioluminescent bacterium Aliivibrio fischeri is one of the best ... B) The symbiosis between bobtail squid and the marine bacterium Aliivibrio fischeri is fundamental to understanding host and ...
https://en.wikipedia.org/wiki/Microbiome
Isaac ChoyIsaac Choy
... and later contested in 2014 by Senator Glenn Wakai's SB3124 bill proposing Aliivibrio fischeri instead. As of December 2017[ ...
https://en.wikipedia.org/wiki/Isaac_Choy
Brian TaniguchiBrian Taniguchi
... and later contested in 2014 by Senator Glenn Wakai's SB3124 bill proposing Aliivibrio fischeri instead. As of December 2017[ ...
https://en.wikipedia.org/wiki/Brian_Taniguchi
ReflectinReflectin
An additional ancestor could be symbiotic Vibrio fischeri (also called Aliivibrio fischeri) which is a bioluminescent (produces ... As reflectin and Vibrio fischeri share similar functions such as producing an iridescent appearance in organisms, it is also ... thought that, just like Vibrio fischeri, Reflectin is symbiotic and is used by cephalopods to interact with their environment. ...
https://en.wikipedia.org/wiki/Reflectin
Spot 42 RNASpot 42 RNA
Aliivibrio fischeri and Aliivibrio salmonicida the spf gene is flanked by polA (upstream) and a sRNA gene encoding the novel ... "Expression profiling reveals Spot 42 small RNA as a key regulator in the central metabolism of Aliivibrio salmonicida". BMC ... Aliivibrio, Photobacterium and Grimontia genera). In e.g., Vibrio cholerae, Vibrio vulnificus, ... and the majority of experimental work on Spot42 has been performed in Escherichia coli and recently in Aliivibrio salmonicida. ...
https://en.wikipedia.org/wiki/Spot_42_RNA
Siouxsie WilesSiouxsie Wiles
... which featured Hawaiian bobtail squid and Aliivibrio fischeri. Wiles thinks that relevant science education should start in ...
https://en.wikipedia.org/wiki/Siouxsie_Wiles
SquidSquid
Aliivibrio fischeri) that produce light to help the squid avoid nocturnal predators. This light shines through the squid's skin ... a special light organ in the squid's mantle is rapidly colonized with Aliivibrio fischeri bacteria within hours of hatching. ... fischeri. Colonization occurs in a horizontal manner, such that the hosts acquires its bacterial partners from the environment ...
https://en.wikipedia.org/wiki/Squid
Vibrio harveyiVibrio harveyi
Quorum sensing was first studied in V. fischeri (now Aliivibrio fischeri), a marine bacterium that uses a synthase (LuxI) to ... Despite its relatedness to A. fischeri, V. harveyi lacks a LuxI/R quorum-sensing system, and instead employs a hybrid quorum- ...
https://en.wikipedia.org/wiki/Vibrio_harveyi
Synthetic biologySynthetic biology
One such system is the Lux operon of Aliivibrio fischeri, which codes for the enzyme that is the source of bacterial ...
https://en.wikipedia.org/wiki/Synthetic_biology
2014 Ju-Jitsu World Championships2014 Ju-Jitsu World Championships
The 2014 Ju-Jitsu World Championship were the 12th edition of the Ju-Jitsu World Championships, and were held in Paris, France from November 28 to November 30, 2014. 28.11.2014 - Men's and Women's Fighting System, Men's and Women's Jiu-Jitsu (ne-waza), Men's Duo System - Classic 29.11.2014 - Men's and Women's Fighting System, Men's and Women's Jiu-Jitsu (ne-waza), Women's Duo System - Classic 30.11.2014 - Men's Jiu-Jitsu (ne-waza), Mixed Duo System - Classic, Team event Vincent MATCZAK (2014-09-30). "4TH INVITAION TO WORLD CHAMPIONSHIP 2014" (PDF). Retrieved 2019-11-28.[dead link] Online results Official results (PDF) Mixed team event results (PDF) (All articles with dead external links, Articles with dead external links from April 2022, Ju-Jitsu World Championships, 2014 in French sport ...
https://en.wikipedia.org/wiki/2014_Ju-Jitsu_World_Championships
Bolley JohnsonBolley Johnson
Bolley L. "Bo" Johnson (born November 15, 1951) is an American politician from the state of Florida. A member of the Democratic Party, Johnson was a member of the Florida House of Representatives, and served as the Speaker of the Florida House of Representatives. Johnson is from Milton, Florida. His father and grandfather served as county commissioners for Santa Rosa County, Florida. Johnson graduated from Milton High School, and became the first member of his family to attend college. He received his bachelor's degree from Florida State University. Johnson volunteered for Mallory Horne when Horne served as the president of the Florida Senate. At the age of 22, Johnson met Lawton Chiles, then a member of the United States Senate, who hired him as a legislative aide in 1973. Johnson was elected to the Florida House of Representatives, representing the 4th district from November 7, 1978 to November 3, 1992. He also served the 1st district from November 3, 1992 to November 8, 1994. He became the ...
https://en.wikipedia.org/wiki/Bolley_Johnson
Toxicities of Four Parabens and Their Mixtures to Daphnia magna and Aliivibrio fischeriToxicities of Four Parabens and Their Mixtures to Daphnia magna and Aliivibrio fischeri
However, EC50 value does not mean killing A. fischeri. To demonstrate this, we confirmed that the growth of A. fischeri was ... Toxicities of Four Parabens and Their Mixtures to Daphnia magna and Aliivibrio fischeri ... Daphnia magna and Aliivibrio fischeri. Parabens are one of the widely used preservatives for personal care products, such as ... fischeri culture. A. fischeri strains were grown at 180 rpm and 30°C until OD600 reached 0.6-0.7. After that, bioluminescence ...
https://www.eaht.org/journal/view.php?number=820
View source for Taxonomy Index - microbewiki
Aliivibrio fischeri]],br> [[Anabaena]],br> [[Aquifex]],br> [[Arthrobacter]],br> [[Azoarcus]],br> [[ ...
https://microbewiki.kenyon.edu/index.php?title=Taxonomy_Index&action=edit
General baseline toxicity QSAR for nonpolar, polar and ionisable chemicals and their mixtures in the bioluminescence inhibition...General baseline toxicity QSAR for nonpolar, polar and ionisable chemicals and their mixtures in the bioluminescence inhibition...
... a bioluminescence inhibition assay with the marine bacterium Aliivibrio fischeri, is one of the most popular bioassays for ... The Microtox assay, a bioluminescence inhibition assay with the marine bacterium Aliivibrio fischeri, is one of the most ... polar and ionisable chemicals and their mixtures in the bioluminescence inhibition assay with Aliivibrio fischeri† ... polar and ionisable chemicals and their mixtures in the bioluminescence inhibition assay with Aliivibrio fischeri B. I. Escher ...
https://pubs.rsc.org/en/Content/ArticleLanding/2017/EM/C6EM00692B
Green Pages · The Global Directory for Environmental Technology › Current Listings
Toxicity Test Kits, Aliivibrio fischeri (formerly Vibrio f.), luminescence, Microbiotests, ATP Test Kits, Algal Toxin Tests. ...
https://eco-web.com/reg/index.html
Release of substances from joint grouts based on various binder types and their ecotoxic effects | Environmental Sciences...Release of substances from joint grouts based on various binder types and their ecotoxic effects | Environmental Sciences...
Several of the identified substances caused bacterial toxicity to Aliivibrio fischeri.. Eluates from fire-retardant coatings- ... liquid-dried luminescent bacteria while measuring the decrease in luminescence of the marine bacterium Aliivibrio fischeri ( ... formerly Vibrio fischeri) after an exposure time of 30 min. The fish egg test was carried out in accordance with ISO 15088 ( ... Water quality-determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (luminescent ...
https://enveurope.springeropen.com/articles/10.1186/s12302-022-00686-0
Toxics | Free Full-Text | Additivity and Interactions in Ecotoxicity of Pollutant Mixtures: Some Patterns, Conclusions, and...Toxics | Free Full-Text | Additivity and Interactions in Ecotoxicity of Pollutant Mixtures: Some Patterns, Conclusions, and...
As model organisms they used a battery of aquatic organisms, including a natural bioluminescent organism (Aliivibrio fischeri ... fischeri, Pseudomonas putida, the protozoan Tetrahymena thermophila and activated sludge. Nine of the binary combinations ... The single substance and mixture toxicity of quinolones to the bioluminescent bacterium vibrio fischeri. Aquat. Toxicol. 2000, ... Predictability of the toxicity of multiple chemical mixtures to vibrio fischeri: Mixtures composed of similarly acting ...
https://www.mdpi.com/2305-6304/3/4/342
MESH TREE NUMBER CHANGES - 2012 MeSH. August 19, 2011MESH TREE NUMBER CHANGES - 2012 MeSH. August 19, 2011
Replaced for 2012 by Aliivibrio fischeri) Vibrio salmonicida B3.440.450.900.859.745 B3.440.450.900.50.930 B3.660.250.830. ... E4.555.165.500 Vibrio fischeri B3.440.450.900.859.300 B3.440.450.900.50.860 B3.660.250.830.830.250 B3.660.250.830.50.860 ( ... 830.745 B3.660.250.830.50.930 (Replaced for 2012 by Aliivibrio salmonicida) Viral Load E1.370.225.875.950 Virus Cultivation ...
https://decs.bvs.br/P/mnchg2012.txt
DeCS 2012 - Changed terms
Vibrio fischeri. Aliivibrio fischeri. Vibrio salmonicida. Aliivibrio salmonicida. Yersinia rucker. Yersinia ruckeri. ...
https://decs2014.bvsalud.org/I/rep_i2012.htm
DeCS 2012 - Changed terms
Vibrio fischeri. Aliivibrio fischeri. Vibrio salmonicida. Aliivibrio salmonicida. Yersinia rucker. Yersinia ruckeri. ...
https://decs2013.bvsalud.org/I/rep_i2012.htm
DeCS 2012 - Changed terms
Vibrio fischeri. Aliivibrio fischeri. Vibrio salmonicida. Aliivibrio salmonicida. Yersinia rucker. Yersinia ruckeri. ...
https://decs2018.bvsalud.org/I/rep_i2012.htm
DeCS 2012 - Changed terms
Vibrio fischeri. Aliivibrio fischeri. Vibrio salmonicida. Aliivibrio salmonicida. Yersinia rucker. Yersinia ruckeri. ...
https://decs2012.bvsalud.org/I/rep_i2012.htm
DeCS 2012 - Changed termsDeCS 2012 - Changed terms
Vibrio fischeri. Aliivibrio fischeri. Vibrio salmonicida. Aliivibrio salmonicida. Yersinia rucker. Yersinia ruckeri. ...
https://decs.bvsalud.org/I/rep_i2012.htm
DeCS 2012 - Changed terms
Vibrio fischeri. Aliivibrio fischeri. Vibrio salmonicida. Aliivibrio salmonicida. Yersinia rucker. Yersinia ruckeri. ...
https://decs2020.bvsalud.org/I/rep_i2012.htm
DeCS 2012 - Changed terms
Vibrio fischeri. Aliivibrio fischeri. Vibrio salmonicida. Aliivibrio salmonicida. Yersinia rucker. Yersinia ruckeri. ...
https://decs2015.bvsalud.org/I/rep_i2012.htm
DeCS 2012 - Changed terms
Vibrio fischeri. Aliivibrio fischeri. Vibrio salmonicida. Aliivibrio salmonicida. Yersinia rucker. Yersinia ruckeri. ...
https://decs2017.bvsalud.org/I/rep_i2012.htm
DeCS 2012 - Changed terms
Vibrio fischeri. Aliivibrio fischeri. Vibrio salmonicida. Aliivibrio salmonicida. Yersinia rucker. Yersinia ruckeri. ...
https://decs2016.bvsalud.org/I/rep_i2012.htm
DeCS 2012 - Changed terms
Vibrio fischeri. Aliivibrio fischeri. Vibrio salmonicida. Aliivibrio salmonicida. Yersinia rucker. Yersinia ruckeri. ...
https://decs2019.bvsalud.org/I/rep_i2012.htm
Code System Concept
Photobacterium fischeri Active Synonym false false 3671779012 Aliivibrio fischeri Active Synonym false false ...
https://phinvads.cdc.gov/vads/ViewCodeSystemConcept.action?oid=2.16.840.1.113883.6.96&code=115050000
Pesquisa | Portal Regional da BVSPesquisa | Portal Regional da BVS
Aliivibrio fischeri , Burkholderia cepacia , Aliivibrio fischeri/química , Aliivibrio fischeri/metabolismo , Cromatografia ... Aliivibrio fischeri/genética , Aliivibrio fischeri/metabolismo , Fenótipo , Transcriptoma , Animais , Antioxidantes , Biofilmes ... Aliivibrio fischeri/genética , Aliivibrio fischeri/metabolismo , Proteínas de Bactérias/genética , DNA/metabolismo , ... Aliivibrio fischeri/metabolismo , Proteínas de Bactérias/metabolismo , Fatores de Transcrição/metabolismo , Aliivibrio fischeri ...
https://pesquisa.bvsalud.org/portal/?lang=pt&q=mh:Aliivibrio+fischeri/metabolismo
Genomes encoding eukaryotic-like proteins | EffectiveDBGenomes encoding eukaryotic-like proteins | EffectiveDB
Aliivibrio fischeri. s. 4. 1. Aliivibrio fischeri. s. 4. 1. Aliivibrio fischeri. s. 4. 1. ...
https://effectors.org/reports/eld_report?domainid=PF01786
roberta-martinoli-nutrizionista.itroberta-martinoli-nutrizionista.it
Microbiology from A to Z explained - Micropia - MicropiaMicrobiology from A to Z explained - Micropia - Micropia
The bioluminescent bacterium Aliivibrio fischeri for example, will only light up if there are enough of its peers around. ...
https://www.micropia.nl/en/discover/microbiology/quorum-sensing/
Pre GI: Gene
NC_011184:2421687 Vibrio fischeri MJ11 chromosome I, complete sequence. Host Lineage: Aliivibrio fischeri; Aliivibrio; ...
http://pregi.bi.up.ac.za/pre_gi_gene.php?source_accession=NC_011184&gi_accession=NC_011184:2421687
B6db families : 4 1 1 11
Aliivibrio fischeri ES114); ... gene functions in the marine bacterium Vibrio fischeri J Biol ...
http://bioinformatics.unipr.it/cgi-bin/bioinformatics/B6db/fam.pl/Show?_id=4_1_1_11&sort=U1&search=FT%3A1&hits=272
Brock Biology Of Microorganis 15 Th ed by. Madigan, Kelly S. Bender -Test Bank | INSTANT DOWNLOAD AFTER PURCHASED - Test Bank...Brock Biology Of Microorganis 15 Th ed by. Madigan, Kelly S. Bender -Test Bank | INSTANT DOWNLOAD AFTER PURCHASED - Test Bank...
D) light emission by Aliivibrio fischeri.. Answer: CBlooms Taxonomy: 5-6: Evaluating/CreatingChapter Section: 6.8 24) ... Answer: CBlooms Taxonomy: 5-6: Evaluating/CreatingChapter Section: 6.410) During a growth curve of Aliivibrio fischeri, when ...
https://testbankdeed.com/product/brock-biology-of-microorganis-15-th-ed-by-madigan-kelly-s-bender-test-bank-instant-download-after-purchased/
Pages without language links - Medien WikiPages without language links - Medien Wiki
Paola S. Calderón/Project: Specimen Physarum Polycephalum./Investigation around Aliivibrio Fischeri.. *Paola S. Calderón/ ... Paola S. Calderón/Project: Specimen Physarum Polycephalum./Diary of work: Alii Vibrio Fischeri ...
https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=Special:WithoutInterwiki&limit=500&offset=250
Team:CU-Boulder/Safety - 2012.igem.orgTeam:CU-Boulder/Safety - 2012.igem.org
Our project aims to harness the bioluminescence genes, known as the Lux operon, from Aliivibrio fischeri, to make a genetically ...
https://2012.igem.org/wiki/index.php?title=Team:CU-Boulder/Safety&direction=next&oldid=14099
孙昊宇-上海大学环境与化学工程
Investigations on the influence of energy source on time-dependent hormesis: A case study of sulfadoxine to Aliivibrio fischeri ... A case study of the mixture of sulfonamide and quorum sensing inhibitor to bioluminescence of Aliivibrio fischeri, Science of ... A case study of binary antibacterial mixtures to Aliivibrio fischeri, Ecotoxicology and Environmental Safety, 187 (2020): ... A case study on the toxicity of antibacterial chemicals to Aliivibrio fischeri, Chemosphere, 205 (2018): 15-23. ...
https://ece.shu.edu.cn/content2.jsp?urltype=news.NewsContentUrl&wbtreeid=1144&wbnewsid=4590
April | 2018 | IL Receptor
... and Aliivibrio sifiae strain Y1 [formerly known as Vibrio fischeri strain Y1]). The mechanism of this phenomenon was initially ... another case, an accessory yellow fluorescent protein (YFP) was discovered Midostaurin in the yellow-light-emitting V. fischeri ...
https://ilreceptor.com/2018/04
A small French town will be lit by bioluminescent organismsA small French town will be lit by bioluminescent organisms
Glowee, on the other hand, employs a marine bacterium called Aliivibrio fischeri collected off the coast of France to light up ...
https://interestingengineering.com/science/french-town-bioluminescent-organisms
BacteriumDaphniaMagnaMixturesBioluminescence inhibitionGenesSystemsVibrioBacterium Aliivibrio fischeriBioluminescent bacteriumLuminescent bacteriaBacteriaBioluminescenceInhibitionSalmonicidaToxicityAchromobacterEuprymnaStrainEnvironmental
Bacterium5
  • The Microtox assay, a bioluminescence inhibition assay with the marine bacterium Aliivibrio fischeri , is one of the most popular bioassays for assessing the cytotoxicity of organic chemicals, mixtures and environmental samples. (rsc.org)
  • The marine bacterium Vibrio fischeri and the freshwater crustacean Daphnia magna were employed as aquatic bioindicators, while for the soil ecosystem, the seeds of Sorghum saccharatum and Lepidium sativum were used. (bvsalud.org)
  • The bioluminescent bacterium Aliivibrio fischeri for example, will only light up if there are enough of its peers around. (micropia.nl)
  • Pan S, Nikolakakis K, Adamczyk PA, Pan M, Ruby EG, Reed JL (2017) Model-enabled gene search (MEGS) allows fast and direct discovery of enzymatic and transport gene functions in the marine bacterium Vibrio fischeri J Biol Chem 292 10250-10261. (unipr.it)
  • Glowee, on the other hand, employs a marine bacterium called Aliivibrio fischeri collected off the coast of France to light up rooms and, potentially, whole cities in the future. (interestingengineering.com)
Daphnia2
  • BP) and their mixtures to two aquatic microorganisms, Daphnia magna and Aliivibrio fischeri . (eaht.org)
  • The ecotoxicity (Daphnia magna, Aliivibrio fischeri, Raphidocelis subcapitata, and Sorghum saccharatum) was absent or presented very slight acute toxicity up to 12.5 mg/L being S. anteuphorbium the least toxic. (unina.it)
Magna1
  • Results indicated V. fischeri as the most sensitive of all the species tested (5.56 g/L), while D. magna was found to be affected at 94.27 g/L. An overall inhibition was observed in seedling growth as compared to control at the highest concentration of the pollutants (100 g/L), while seed germination was not adversely affected by the pollutant. (bvsalud.org)
Mixtures1
  • Regular time-dependent cross-phenomena induced by hormesis: A case study of binary antibacterial mixtures to Aliivibrio fischeri , Ecotoxicology and Environmental Safety , 187 (2020): 109823. (shu.edu.cn)
Bioluminescence inhibition3
  • The results showed their value of MP (25.2 mg/L, 73.4 mg/L), EP (18.4 mg/L, 43.7 mg/L), PP (10.4 mg/L, 21.1 mg/L) and BP (3.3 mg/L, 11.2 mg/L). Then, each of the parabens was treated to A. fischeri and calculated their EC 20 and EC 50 by bioluminescence inhibition test. (eaht.org)
  • As a result, the bioluminescence inhibition test of A. fischeri showed that the toxicity of mixture parabens was stronger than that of a single compound and combinations of three parabens showed the highest bioluminescence inhibition. (eaht.org)
  • Compared to acute toxicity test using freshwater daphnids, bioluminescence inhibition test using A. fischeri requires shorter exposure time, smaller sample volume, easier skills. (eaht.org)
Genes2
  • To begin to decode the functions of the 50 Vibrio fischeri genes with GGDEF, EAL, and/or HD-GYP domains, we deleted each gene and assessed each mutant's migration through tryptone broth salt (TBS) soft agar medium containing or lacking magnesium (Mg) and calcium (Ca), which are known to influence V. fischeri motility. (bvsalud.org)
  • Our project aims to harness the bioluminescence genes, known as the Lux operon, from Aliivibrio fischeri, to make a genetically engineered E. Coli machine. (igem.org)
Systems1
  • 2. Qing Gao, Jing Wang, Longfei Ren, Yifei Cheng, Zhifen Lin, Xin-Gui Li, Haoyu Sun * . Investigations on the influence of energy source on time-dependent hormesis: A case study of sulfadoxine to Aliivibrio fischeri in different cultivation systems, Science of The Total Environment , 775 (2021): 145877. (shu.edu.cn)
Vibrio12
  • Vibrio fischeri (Beijerinck 1889) Lehmann and Neumann 1896 (Approved Lists 1980), nom. (nih.gov)
  • Vibrio fischeri and Photobacterium fischeri are homotypic synonyms. (nih.gov)
  • One important method for assessing toxicity quickly and cost effectively is a bioluminescence inhibition assay that employs a marine gram negative bacterium, Aliivibrio fischeri (formerly Vibrio fischeri) as the test species. (biotoxicity.com)
  • ISO 11348-3: Water quality - Determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (luminescent bacteria test). (biotoxicity.com)
  • The WaterTox STD (Standard) kit is packaged to provide users with a complete setup to replace traditional vibrio fischeri acute toxicity tests (Microtox® users). (biotoxicity.com)
  • This method is based on ISO 11348-3: Water quality - Determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (luminescent bacteria test). (biotoxicity.com)
  • The mixture toxicity against selected bacteria was assessed using assays with Pseudomonas putida and Vibrio fischeri. (nih.gov)
  • The toxicities were estimated by three tests in silico using the TEST software and in vitro bioassays using Vibrio fischeri bacteria. (nih.gov)
  • We studied the Euprymna scolopes-Vibrio fischeri symbiosis to characterize, in vivo and in real time, the transition between the bacterial partner's free-living and symbiotic life styles. (birmingham.ac.uk)
  • A total of six recombinant Escherichia coli strains with the promoters from three oxidative-stress responsive genes, i.e. the katG, sodA and pqi-5 genes, fused to either the lux genes from Vibrio fischeri or X. luminescens were characterized and their responses to different chemicals compared. (elsevier.com)
  • Esta nueva linea de producto basada en la bacteria bioluminiscente Aliivibrio fischeri (anteriormente Vibrio fischeri) permite evaluar la ecotoxicidad de muestras de matrices ambientales de una forma rápida. (ecotestsl.com)
  • Spraying, wiping, a… The toxic effects of five Quaternary Ammonium Compounds (QACs) that are widely used as active ingredients in personal care products were assessed using the bioluminescent bacterium Aliivibrio fischeri (formerly Vibrio fischeri) (Microtox® test system). (clinicalcareer.co.uk)
Bacterium Aliivibrio fischeri2
  • Numerous organisms, including the marine bacterium Aliivibrio fischeri, produce light. (emerginginvestigators.org)
  • In this study, acute toxicity of As(III), As(V), In(III) and Ga(III) species was evaluated using two microbial assays testing for methanogenic activity and O 2 uptake, as well as two bioassays targeting aquatic organisms, including the marine bacterium Aliivibrio fischeri (bioluminescence inhibition) and the crustacean Daphnia magna (mortality). (elsevier.com)
Bioluminescent bacterium1
  • A chunk of DNA from a bioluminescent bacterium called Aliivibrio fischeri was inserted alongside genes encoding resistance to particular metals. (rosincerate.com)
Luminescent bacteria1
  • A simple nanopaper-based platform coupled with luminescent bacteria Aliivibrio fischeri (A. fischeri) as a bio-indicator is presented here, for rapid and sensitive evaluation of contaminant toxicity. (uab.cat)
Bacteria1
  • A species of gram-negative bacteria in the genus ALIIVIBRIO , which exhibits LUMINESCENCE. (nih.gov)
Bioluminescence3
  • It was found that the basal level bioluminescence (BL) from the thermo-lux fusion strains was always higher while that of the V. fischeri lux strains were always near or below the lower limit of detection of the system. (elsevier.com)
  • The use of the thermo-lux strains found them to be as sensitive as the V. fischeri lux strains while providing a brighter, more stable basal level bioluminescence, making the analysis and monitoring of water-borne toxicity more reliable. (elsevier.com)
  • Our project aims to harness the bioluminescence genes, known as the Lux operon, from Aliivibrio fischeri, to make a genetically engineered E. Coli machine. (igem.org)
Inhibition1
  • When exposed to toxicants, the luminescence inhibition of A. fischeri-decorated bioluminescent nanopaper (BLN) can be quantified and analyzed to classify the toxicity level of a pollutant. (uab.cat)
Salmonicida1
  • Aliivibrio salmonicida comb. (nih.gov)
Toxicity1
  • EBPI is currently carrying several preparations of high quality Aliivibrio fischeri test reagents from Finnish manufacture Aboatox to conduct rapid toxicity assessments in a wide range of environmental samples. (biotoxicity.com)
Achromobacter1
  • 'Achromobacter fischeri' (Beijerinck 1889) Bergey et al. (nih.gov)
Euprymna1
  • A. fischeri is found in a symbiotic relationship with the SQUID Euprymna scolopes. (nih.gov)
Strain1
  • V. fischeri lux strain, DPD2511, gave no discernible response due to its low level expression while a fusion of the katG promoter with the X. luminescens lux operon was clearly responsive and capable of detecting hydrogen peroxide down to about 1 ppm. (elsevier.com)
Environmental1
  • Previous studies using high inocula demonstrated that environmental V. fischeri cells aggregate during a 3 h period in host-shed mucus along the light organ's superficial ciliated epithelia. (birmingham.ac.uk)
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