A genus of the family BROMOVIRIDAE with a wide host range. Transmission is by aphids and the type species is ALFALFA MOSAIC VIRUS.

A conserved hairpin structure in Alfamovirus and Bromovirus subgenomic promoters is required for efficient RNA synthesis in vitro. (1/3)

The coat protein gene in RNA 3 of alfalfa mosaic virus (AMV; genus Alfamovirus, family Bromoviridae) is translated from the subgenomic RNA 4. Analysis of the subgenomic promoter (sgp) in minus-strand RNA 3 showed that a sequence of 37 nt upstream of the RNA 4 start site (nt +1) was sufficient for full sgp activity in an in vitro assay with the purified viral RNA-dependent RNA-polymerase (RdRp). The sequence of nt -6 to -29 could be folded into a potential hairpin structure with a loop represented by nt -16, -17, and -18, and a bulge involving nt -23. By introducing mutations that disrupted base pairing and compensatory mutations that restored base pairing, it was shown that base pairing in the top half of the putative stem (between the loop and bulge) was essential for sgp activity, whereas base pairing in the bottom half of the stem was less stringently required. Deletion of the bulged residue A-23 or mutation of this residue into a C strongly reduced sgp activity, but mutation of A-23 into U or G had little effect on sgp activity. Mutation of loop residues A-16 and A-17 affected sgp activity, whereas mutation of U-18 did not. Using RNA templates corresponding to the sgp of brome mosaic virus (BMV; genus Bromovirus, family Bromoviridae) and purified BMV RdRp, evidence was obtained indicating that also in BMV RNA a triloop hairpin structure is required for sgp activity.  (+info)

Antigens produced in plants by infection with chimeric plant viruses immunize against rabies virus and HIV-1. (2/3)

The coat protein (CP) of alfalfa mosaic virus was used as a carrier molecule to express antigenic peptides from rabies virus and HIV. The antigens were separately cloned into the reading frame of alfalfa mosaic virus CP and placed under the control of the subgenomic promoter of tobacco mosaic virus CP in the 30BRz vector. The in vitro transcripts of recombinant virus with sequences encoding the antigenic peptides were synthesized from DNA constructs and used to inoculate tobacco plants. The plant-produced protein (virus particles) was purified and used for immunization of mice. Both antigens elicited specific virus-neutralizing antibodies in immunized mice.  (+info)

Tubule-forming capacity of the movement proteins of alfalfa mosaic virus and brome mosaic virus. (3/3)

The structural phenotype of the movement proteins (MPs) of two representatives of the Bromoviridae, alfalfa mosaic virus (AMV) and brome mosaic virus (BMV), was studied in protoplasts. Immunofluorescence microscopy showed that the MPs of these viruses, for which there has been no evidence of a tubule-guided mechanism, assemble into long tubular structures at the surface of the infected protoplast. Electron microscopy and immunogold analysis confirmed the presence of both MP and virus particles in the tubules induced by AMV and BMV. The significance of the tubule-forming properties of these viral MPs is discussed.  (+info)

ID HQ332378; SV 1; linear; genomic RNA; STD; VRL; 311 BP. XX AC HQ332378; XX DT 01-AUG-2011 (Rel. 109, Created) DT 01-AUG-2011 (Rel. 109, Last updated, Version 1) XX DE Alfalfa mosaic virus isolate DA-03 coat protein-like gene, partial DE sequence. XX KW . XX OS Alfalfa mosaic virus OC Viruses; Riboviria; Bromoviridae; Alfamovirus. XX RN [1] RP 1-311 RA Degirmenci K., Akbas B.; RT Characterization of different Alfalfa mosaic virus isolates; RL Unpublished. XX RN [2] RP 1-311 RA Degirmenci K., Akbas B.; RT ; RL Submitted (24-SEP-2010) to the INSDC. RL Phytopathology, Plant Protection Central Research Institute, Fatih Sultan RL Mehmet Bulvari No: 66, Ankara 06172, Turkey XX DR MD5; 29edf3d5d3e6115579c83b09ee13dfd0. XX FH Key Location/Qualifiers FH FT source 1..311 FT /organism=Alfalfa mosaic virus FT /host=Medicago sativa (alfalfa) FT /isolate=DA-03 FT /mol_type=genomic RNA FT /country=Turkey FT /collection_date=Apr-2010 FT /db_xref=taxon:12321 FT misc_feature ...
ID JX857635; SV 1; linear; genomic RNA; STD; VRL; 302 BP. XX AC JX857635; XX DT 18-NOV-2012 (Rel. 114, Created) DT 18-NOV-2012 (Rel. 114, Last updated, Version 1) XX DE Alfalfa mosaic virus isolate LZ1 coat protein gene, partial cds. XX KW . XX OS Alfalfa mosaic virus OC Viruses; ssRNA viruses; ssRNA positive-strand viruses, no DNA stage; OC Bromoviridae; Alfamovirus. XX RN [1] RP 1-302 RA Zhaohui W., Keqin H.; RT Identification of viral pathogens inducing Medicago sativa mosaic disease RT in Zhangye Gansu; RL Unpublished. XX RN [2] RP 1-302 RA Zhaohui W., Keqin H.; RT ; RL Submitted (22-SEP-2012) to the INSDC. RL Central Laboratory of Technical Center, Gansu Exit-Entry Inspection and RL Quarantine Bureau, No. 2168, Nanhe Road, Lanzhou, Gansu 730010, China XX DR MD5; 6cbfd4060891d7958346d37c49178c70. XX FH Key Location/Qualifiers FH FT source 1..302 FT /organism=Alfalfa mosaic virus FT /host=Medicago sativa FT /isolate=LZ1 FT /mol_type=genomic RNA FT /country=China FT ...
387440791 - EP 0832191 A4 2000-11-15 - RECOMBINANT VIRAL NUCLEIC ACIDS - [origin: WO9640867A1] The present invention relates to a recombinant viral nucleic acid selected from a (+) sense, single stranded RNA virus possessing a native subgenomic promoter encoding for a first viral subgenomic promoter, a nucleic acid sequence that codes for a viral coat protein whose transcription is regulated by the first viral subgenomic promoter, a second viral subgenomic promoter and a second nucleic acid sequence whose transcription is regulated by the second viral subgenomic promoter. The first and second viral subgenomic promoters of the recombinant viral nucleic acid do not have homologous sequences relative to each other. The recombinant viral nucleic acid provides the particular advantage that it systematically transcribes the second nucleic acid in the host. Host organisms encompassed by the present invention include procaryotes and eucaryotes, particularly animals and plants. The present invention also relates
TY - JOUR. T1 - Molecular and partial biological characterization of the coat protein sequences of Iranian alfalfa mosaic virus isolates. AU - Mangeli, Fatemeh. AU - Massumi, Hossain. AU - Alipour, Fereshteh. AU - Maddahian, Mohammad. AU - Heydarnejad, Jahangir. AU - Hosseinipour, Akbar. AU - Amid-Motlagh, Mohammad Hadi. AU - Azizizadeh, Morteza. AU - Varsani, Arvind. N1 - Funding Information: This research work was supported by the Iran National Science Foundation (INSF; Grant No. 93004315). PY - 2019/8/15. Y1 - 2019/8/15. N2 - Alfalfa mosaic virus (AMV) is one of the most important viruses that cause significant losses to alfalfa worldwide. A total of 1066 symptomatic alfalfa, 143 weeds and other secondary hosts were collected and assayed for AMV infection. The virus was detected in 360 samples (33.8% incidence) of alfalfa by plate-trapped antigen (PTA)-ELISA. Among the weed species and the other plants tested, potato, pepper and seven weed species were infected with AMV. The coat protein (CP) ...
Phytopathology 91:941-947...Phytopathology 91:941-947...Pathogenesis of Alfalfa mosaic virus in Soybean (Glycine max) and Expression of Chimeric Rabies Peptide in Virus-Infected Soybean Plants...Nina Fleysh , Deepali Deka , Maria Drath , Hilary Koprowski , and Vidadi Yusibov...
It is the only Alfamovirus of the family Bromoviridae. In 1931 Weimer J.L. was the first to report AMV in alfalfa (Medicago ...
"Efficient translation of alfamovirus RNAs requires the binding of coat protein dimers to the 3′ termini of the viral RNAs". The ...
The following genera are assigned to the family: Alfamovirus Anulavirus Bromovirus Cucumovirus Ilarvirus Oleavirus Viruses in ...
Ahduovirus Ahphunavirus Ahtivirus Ailurivirus Albetovirus Alcyoneusvirus Alefpapillomavirus Alexandravirus Alfamovirus ...
... alfamovirus MeSH B04.820.464.040.050 - alfalfa mosaic virus MeSH B04.820.464.080 - bromovirus MeSH B04.820.464.150 - comovirus ... alfamovirus MeSH B04.715.081.050.050 - alfalfa mosaic virus MeSH B04.715.081.080 - bromovirus MeSH B04.715.081.180 - ... alfamovirus MeSH B04.715.464.040.050 - alfalfa mosaic virus MeSH B04.715.464.080 - bromovirus MeSH B04.715.464.100 - ... alfamovirus MeSH B04.820.081.050.050 - alfalfa mosaic virus MeSH B04.820.081.080 - bromovirus MeSH B04.820.081.180 - ...
virus (genus Alfamovirus, family Bormoviridae), Cucumber mosaic virus (genus Cucumovirus, family Bormoviridae), and Southern ...
B4.820.464.40.50 Alfamovirus B4.820.464.40 Alkane 1-Monooxygenase D8.811.682.690.708.170.35 D8.244.453.450 D8.811.682.690. ...
Genus Alfamovirus. Type species Alfalfa mosaic virus. Distinguishing features. Alfamoviruses are transmitted in a non- ... The 3′ termini of the RNAs are similar to those of the genera Alfamovirus and Ilarvirus, but do not interact with CP to ... A longstanding debate as to whether AMV (genus Alfamovirus) should be regarded as a member of the genus Ilarvirus has yet to be ... List of other related viruses which may be members of the genus Alfamovirus but have not been approved as species. ...
Alfamovirus - Preferred Concept UI. M0028581. Scope note. A genus of the family BROMOVIRIDAE with a wide host range. ... Alfamovirus. Scope note:. Género de la familia BROMOVIRIDAE con una amplia gama de hospedadores. La transmisión se produce por ...
Alfamovirus Active Synonym false false 2648212013 Genus Alfamovirus Active Synonym false false ...
... alfamovirus, and tymovirus groups. We have optimized a non-radioactive nucleic acid hybridization (NASH) assay for the ...
We have previously reported the presence of m6A in the AMV (Alfamovirus, Bromoviridae) genome. Interestingly, two of these ...
... but the samples also contained Alfalfa mosaic alfamovirus (AMV), Cucumber mosaic ClIC11171014171S (CMV) and Potato Y potyvirus ...
B4.820.464.40.50 Alfamovirus B4.820.464.40 Alkane 1-Monooxygenase D8.811.682.690.708.170.35 D8.244.453.450 D8.811.682.690. ...
B4.820.464.40.50 Alfamovirus B4.820.464.40 Alkane 1-Monooxygenase D8.811.682.690.708.170.35 D8.244.453.450 D8.811.682.690. ...
B4.820.464.40.50 Alfamovirus B4.820.464.40 Alkane 1-Monooxygenase D8.811.682.690.708.170.35 D8.244.453.450 D8.811.682.690. ...
B4.820.464.40.50 Alfamovirus B4.820.464.40 Alkane 1-Monooxygenase D8.811.682.690.708.170.35 D8.244.453.450 D8.811.682.690. ...
B4.820.464.40.50 Alfamovirus B4.820.464.40 Alkane 1-Monooxygenase D8.811.682.690.708.170.35 D8.244.453.450 D8.811.682.690. ...
B4.820.464.40.50 Alfamovirus B4.820.464.40 Alkane 1-Monooxygenase D8.811.682.690.708.170.35 D8.244.453.450 D8.811.682.690. ...
Genus Alfamovirus; Alfalfa mosaic virus (AMV). Andean potato mottle. Genus Comovirus; Andean potato mottle virus (APMoV). Beet ...
Alfamovirus B04.715.081.050.050 Alfalfa mosaic virus B04.715.081.080 Bromovirus B04.715.081.180 Cucumovirus B04.715.081.400 ... Alfamovirus B04.715.464.040.050 Alfalfa mosaic virus B04.715.464.080 Bromovirus B04.715.464.100 Caulimovirus B04.715.464.150 ... Alfamovirus B04.820.081.050.050 Alfalfa mosaic virus B04.820.081.080 Bromovirus B04.820.081.180 Cucumovirus B04.820.081.400 ...
Alfamovirus; Plantas Huéspedes; Diversidad Genética (como recurso); Variación Genética; Filogenética; Phylogenetic ... Alfalfa mosaic virus (AMV) is a plant virus belonging to the genus Alfamovirus in the family Bromoviridae. AMV occurs worldwide ... Alfalfa mosaic virus (AMV) is a plant virus belonging to the genus Alfamovirus in the family Bromoviridae. AMV occurs worldwide ...
Alfamovirus Preferred Term Term UI T057183. Date01/01/1999. LexicalTag ABX. ThesaurusID NLM (1997). ... Alfamovirus Preferred Concept UI. M0028581. Registry Number. txid12320. Scope Note. A genus of the family BROMOVIRIDAE with a ... Alfamovirus. Tree Number(s). B04.715.081.050. B04.715.464.040. B04.820.578.282.050. Unique ID. D019178. RDF Unique Identifier. ...
Alfamovirus Preferred Term Term UI T057183. Date01/01/1999. LexicalTag ABX. ThesaurusID NLM (1997). ... Alfamovirus Preferred Concept UI. M0028581. Registry Number. txid12320. Scope Note. A genus of the family BROMOVIRIDAE with a ... Alfamovirus. Tree Number(s). B04.715.081.050. B04.715.464.040. B04.820.578.282.050. Unique ID. D019178. RDF Unique Identifier. ...
The genera include: ALFAMOVIRUS; BROMOVIRUS; CUCUMOVIRUS; ILARVIRUS; and OLEAVIRUS. * Alfamovirus 0 domande A genus of the ...
The genera include: ALFAMOVIRUS; BROMOVIRUS; CUCUMOVIRUS; ILARVIRUS; and OLEAVIRUS. * Bunyaviridae 0 domande A family of ...
... alfadolone alfalfa alfalfa caterpillar alfalfa caterpillars alfalfas alfalfa weevil alfalfa weevils alfamovirus Alfamovirus ...
... but the samples also contained Alfalfa mosaic alfamovirus (AMV), Cucumber mosaic ClIC11171014171S (CMV) and Potato Y potyvirus ...
B4.820.464.40.50 Alfamovirus B4.820.464.40 Alkane 1-Monooxygenase D8.811.682.690.708.170.35 D8.244.453.450 D8.811.682.690. ...
B4.820.464.40.50 Alfamovirus B4.820.464.40 Alkane 1-Monooxygenase D8.811.682.690.708.170.35 D8.244.453.450 D8.811.682.690. ...
B4.820.464.40.50 Alfamovirus B4.820.464.40 Alkane 1-Monooxygenase D8.811.682.690.708.170.35 D8.244.453.450 D8.811.682.690. ...
... alfadolone alfalfa alfalfa caterpillar alfalfa caterpillars alfalfas alfalfa weevil alfalfa weevils alfamovirus Alfamovirus ...
B4.820.464.40.50 Alfamovirus B4.820.464.40 Alkane 1-Monooxygenase D8.811.682.690.708.170.35 D8.244.453.450 D8.811.682.690. ...
B4.820.464.40.50 Alfamovirus B4.820.464.40 Alkane 1-Monooxygenase D8.811.682.690.708.170.35 D8.244.453.450 D8.811.682.690. ...
Ten additional viruses are listed in EPPO PM 4/28 Standard: Alfalfa mosaic alfamovirus, Cucumber mosaic cucumovirus, Potato ...
Descritores em Ciências da Saúde
Riboviria / Orthornavirae / Kitrinoviricota / Alsuviricetes / Martellivirales / Bromoviridae / Alfamovirus / Alfalfa mosaic ...
Riboviria / Orthornavirae / Kitrinoviricota / Alsuviricetes / Martellivirales / Bromoviridae / Alfamovirus / Alfalfa mosaic ...
Alfamovirus Alfaxalone Alfadolone Mixture Alfentanil Algal Proteins Algeria Algestone Algestone Acetophenide Alginates ...
  • V) and the Tomato mosaic tobamovirus (ToMV), but the samples also contained Alfalfa mosaic alfamovirus (AMV), Cucumber mosaic ClIC11171014171S (CMV) and Potato Y potyvirus (PVY). (unideb.hu)
  • Alfalfa mosaic virus (AMV) is a plant virus belonging to the genus Alfamovirus in the family Bromoviridae. (gob.ar)
  • V) and the Tomato mosaic tobamovirus (ToMV), but the samples also contained Alfalfa mosaic alfamovirus (AMV), Cucumber mosaic ClIC11171014171S (CMV) and Potato Y potyvirus (PVY). (unideb.hu)
  • Ten additional viruses are listed in EPPO PM 4/28 Standard: Alfalfa mosaic alfamovirus, Cucumber mosaic cucumovirus, Potato aucuba mosaic potexvirus, Potato mop-top pomovirus, Potato V potyvirus, Tobacco mosaic tobamovirus, Tobacco necrosis necrovirus, Tobacco rattle tobravirus, Tomato black ring nepovirus, and Tomato mosaic tobamovirus. (eppo.int)