A conserved hairpin structure in Alfamovirus and Bromovirus subgenomic promoters is required for efficient RNA synthesis in vitro. (1/3)
The coat protein gene in RNA 3 of alfalfa mosaic virus (AMV; genus Alfamovirus, family Bromoviridae) is translated from the subgenomic RNA 4. Analysis of the subgenomic promoter (sgp) in minus-strand RNA 3 showed that a sequence of 37 nt upstream of the RNA 4 start site (nt +1) was sufficient for full sgp activity in an in vitro assay with the purified viral RNA-dependent RNA-polymerase (RdRp). The sequence of nt -6 to -29 could be folded into a potential hairpin structure with a loop represented by nt -16, -17, and -18, and a bulge involving nt -23. By introducing mutations that disrupted base pairing and compensatory mutations that restored base pairing, it was shown that base pairing in the top half of the putative stem (between the loop and bulge) was essential for sgp activity, whereas base pairing in the bottom half of the stem was less stringently required. Deletion of the bulged residue A-23 or mutation of this residue into a C strongly reduced sgp activity, but mutation of A-23 into U or G had little effect on sgp activity. Mutation of loop residues A-16 and A-17 affected sgp activity, whereas mutation of U-18 did not. Using RNA templates corresponding to the sgp of brome mosaic virus (BMV; genus Bromovirus, family Bromoviridae) and purified BMV RdRp, evidence was obtained indicating that also in BMV RNA a triloop hairpin structure is required for sgp activity. (+info)Antigens produced in plants by infection with chimeric plant viruses immunize against rabies virus and HIV-1. (2/3)
The coat protein (CP) of alfalfa mosaic virus was used as a carrier molecule to express antigenic peptides from rabies virus and HIV. The antigens were separately cloned into the reading frame of alfalfa mosaic virus CP and placed under the control of the subgenomic promoter of tobacco mosaic virus CP in the 30BRz vector. The in vitro transcripts of recombinant virus with sequences encoding the antigenic peptides were synthesized from DNA constructs and used to inoculate tobacco plants. The plant-produced protein (virus particles) was purified and used for immunization of mice. Both antigens elicited specific virus-neutralizing antibodies in immunized mice. (+info)Tubule-forming capacity of the movement proteins of alfalfa mosaic virus and brome mosaic virus. (3/3)
The structural phenotype of the movement proteins (MPs) of two representatives of the Bromoviridae, alfalfa mosaic virus (AMV) and brome mosaic virus (BMV), was studied in protoplasts. Immunofluorescence microscopy showed that the MPs of these viruses, for which there has been no evidence of a tubule-guided mechanism, assemble into long tubular structures at the surface of the infected protoplast. Electron microscopy and immunogold analysis confirmed the presence of both MP and virus particles in the tubules induced by AMV and BMV. The significance of the tubule-forming properties of these viral MPs is discussed. (+info)
ID HQ332378; SV 1; linear; genomic RNA; STD; VRL; 311 BP
ID JX857635; SV 1; linear; genomic RNA; STD; VRL; 302 BP
EP 0832191 A4 20001115 - RECOMBINANT VIRAL NUCLEIC ACIDS
Molecular and partial biological characterization of the coat protein sequences of Iranian alfalfa mosaic virus isolates<...
Pathogenesis of Alfalfa mosaic virus in Soybean (Glycine max) and Expression of Chimeric Rabies Peptide in Virus-Infected...
Alfalfa mosaic virus
It is the only Alfamovirus of the family Bromoviridae. In 1931 Weimer J.L. was the first to report AMV in alfalfa (Medicago ...
Alfalfa mosaic virus coat protein binding (CPB) RNA
"Efficient translation of alfamovirus RNAs requires the binding of coat protein dimers to the 3′ termini of the viral RNAs". The ...
Bromoviridae
The following genera are assigned to the family: Alfamovirus Anulavirus Bromovirus Cucumovirus Ilarvirus Oleavirus Viruses in ...
List of virus genera
Ahduovirus Ahphunavirus Ahtivirus Ailurivirus Albetovirus Alcyoneusvirus Alefpapillomavirus Alexandravirus Alfamovirus ...
List of MeSH codes (B04)
... alfamovirus MeSH B04.820.464.040.050 - alfalfa mosaic virus MeSH B04.820.464.080 - bromovirus MeSH B04.820.464.150 - comovirus ... alfamovirus MeSH B04.715.081.050.050 - alfalfa mosaic virus MeSH B04.715.081.080 - bromovirus MeSH B04.715.081.180 - ... alfamovirus MeSH B04.715.464.040.050 - alfalfa mosaic virus MeSH B04.715.464.080 - bromovirus MeSH B04.715.464.100 - ... alfamovirus MeSH B04.820.081.050.050 - alfalfa mosaic virus MeSH B04.820.081.080 - bromovirus MeSH B04.820.081.180 - ...
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MESH TREE NUMBER CHANGES - 2015 MeSH
Bromoviridae | ICTV
Genus Alfamovirus. Type species Alfalfa mosaic virus. Distinguishing features. Alfamoviruses are transmitted in a non- ... The 3′ termini of the RNAs are similar to those of the genera Alfamovirus and Ilarvirus, but do not interact with CP to ... A longstanding debate as to whether AMV (genus Alfamovirus) should be regarded as a member of the genus Ilarvirus has yet to be ... List of other related viruses which may be members of the genus Alfamovirus but have not been approved as species. ...
DeCS
Code System Concept
Multiplex detection of Potato virus S, Potato virus X, and Potato Virus Y by non-radioactive nucleic acid spot hybridization in...
Viroses/genética
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| International Journal of Horticultural Science
MESH TREE NUMBER CHANGES - 2015 MeSH
MESH TREE NUMBER CHANGES - 2015 MeSH
MESH TREE NUMBER CHANGES - 2015 MeSH
MESH TREE NUMBER CHANGES - 2015 MeSH
MESH TREE NUMBER CHANGES - 2015 MeSH
MESH TREE NUMBER CHANGES - 2015 MeSH
Diseases of Pepper
TREE NUMBER DESCRIPTOR
Alfamovirus B04.715.081.050.050 Alfalfa mosaic virus B04.715.081.080 Bromovirus B04.715.081.180 Cucumovirus B04.715.081.400 ... Alfamovirus B04.715.464.040.050 Alfalfa mosaic virus B04.715.464.080 Bromovirus B04.715.464.100 Caulimovirus B04.715.464.150 ... Alfamovirus B04.820.081.050.050 Alfalfa mosaic virus B04.820.081.080 Bromovirus B04.820.081.180 Cucumovirus B04.820.081.400 ...
Alfalfa mosaic virus (AMV): genetic diversity and a new natural host
Alfamovirus; Plantas Huéspedes; Diversidad Genética (como recurso); Variación Genética; Filogenética; Phylogenetic ... Alfalfa mosaic virus (AMV) is a plant virus belonging to the genus Alfamovirus in the family Bromoviridae. AMV occurs worldwide ... Alfalfa mosaic virus (AMV) is a plant virus belonging to the genus Alfamovirus in the family Bromoviridae. AMV occurs worldwide ...
MeSH Browser
Alfamovirus Preferred Term Term UI T057183. Date01/01/1999. LexicalTag ABX. ThesaurusID NLM (1997). ... Alfamovirus Preferred Concept UI. M0028581. Registry Number. txid12320. Scope Note. A genus of the family BROMOVIRIDAE with a ... Alfamovirus. Tree Number(s). B04.715.081.050. B04.715.464.040. B04.820.578.282.050. Unique ID. D019178. RDF Unique Identifier. ...
MeSH Browser
Alfamovirus Preferred Term Term UI T057183. Date01/01/1999. LexicalTag ABX. ThesaurusID NLM (1997). ... Alfamovirus Preferred Concept UI. M0028581. Registry Number. txid12320. Scope Note. A genus of the family BROMOVIRIDAE with a ... Alfamovirus. Tree Number(s). B04.715.081.050. B04.715.464.040. B04.820.578.282.050. Unique ID. D019178. RDF Unique Identifier. ...
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| International Journal of Horticultural Science
MESH TREE NUMBER CHANGES - 2015 MeSH
MESH TREE NUMBER CHANGES - 2015 MeSH
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MESH TREE NUMBER CHANGES - 2015 MeSH
MESH TREE NUMBER CHANGES - 2015 MeSH
EPPO RNQP
Potyvirus | EVAg
Cowpea mild mottle virus | EVAg
ALFALFA1
- V) and the Tomato mosaic tobamovirus (ToMV), but the samples also contained Alfalfa mosaic alfamovirus (AMV), Cucumber mosaic ClIC11171014171S (CMV) and Potato Y potyvirus (PVY). (unideb.hu)
Alfalfa3
- Alfalfa mosaic virus (AMV) is a plant virus belonging to the genus Alfamovirus in the family Bromoviridae. (gob.ar)
- V) and the Tomato mosaic tobamovirus (ToMV), but the samples also contained Alfalfa mosaic alfamovirus (AMV), Cucumber mosaic ClIC11171014171S (CMV) and Potato Y potyvirus (PVY). (unideb.hu)
- Ten additional viruses are listed in EPPO PM 4/28 Standard: Alfalfa mosaic alfamovirus, Cucumber mosaic cucumovirus, Potato aucuba mosaic potexvirus, Potato mop-top pomovirus, Potato V potyvirus, Tobacco mosaic tobamovirus, Tobacco necrosis necrovirus, Tobacco rattle tobravirus, Tomato black ring nepovirus, and Tomato mosaic tobamovirus. (eppo.int)