KetosesSugar Alcohols: Polyhydric alcohols having no more than one hydroxy group attached to each carbon atom. They are formed by the reduction of the carbonyl group of a sugar to a hydroxyl group.(From Dorland, 28th ed)Aldehyde Reductase: An enzyme that catalyzes reversibly the oxidation of an aldose to an alditol. It possesses broad specificity for many aldoses. EC 1.1.1.21.Aldose-Ketose Isomerases: Enzymes that catalyze the interconversion of aldose and ketose compounds.Isomerases: A class of enzymes that catalyze geometric or structural changes within a molecule to form a single product. The reactions do not involve a net change in the concentrations of compounds other than the substrate and the product.(from Dorland, 28th ed) EC 5.Carbon-Carbon Double Bond Isomerases: Enzymes that catalyze the shifting of a carbon-carbon double bond from one position to another within the same molecule. EC 5.3.3.Peptidylprolyl Isomerase: An enzyme that catalyzes the isomerization of proline residues within proteins. EC 5.2.1.8.Amino Acid Isomerases: Enzymes that catalyze either the racemization or epimerization of chiral centers within amino acids or derivatives. EC 5.1.1.Carbohydrate Epimerases: Enzymes that catalyze the epimerization of chiral centers within carbohydrates or their derivatives. EC 5.1.3.Imidazolidines: Compounds based on reduced IMIDAZOLINES which contain no double bonds in the ring.Protein Disulfide-Isomerases: Sulfur-sulfur bond isomerases that catalyze the rearrangement of disulfide bonds within proteins during folding. Specific protein disulfide-isomerase isoenzymes also occur as subunits of PROCOLLAGEN-PROLINE DIOXYGENASE.Steroid Isomerases: Enzymes that catalyze the transposition of double bond(s) in a steroid molecule. EC 5.3.3.Cyclophilins: A family of peptidyl-prolyl cis-trans isomerases that bind to CYCLOSPORINS and regulate the IMMUNE SYSTEM. EC 5.2.1.-Sorbitol: A polyhydric alcohol with about half the sweetness of sucrose. Sorbitol occurs naturally and is also produced synthetically from glucose. It was formerly used as a diuretic and may still be used as a laxative and in irrigating solutions for some surgical procedures. It is also used in many manufacturing processes, as a pharmaceutical aid, and in several research applications.XyloseEncyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)Lactobacillus: A genus of gram-positive, microaerophilic, rod-shaped bacteria occurring widely in nature. Its species are also part of the many normal flora of the mouth, intestinal tract, and vagina of many mammals, including humans. Pathogenicity from this genus is rare.Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing): An enzyme that catalyzes the synthesis of fructose-6-phosphate plus GLUTAMINE from GLUTAMATE plus glucosamine-6-phosphate.Photorhabdus: A genus of gram-negative bacteria existing symbiotically with nematodes of the family Heterorhabditidae (see RHABDITOIDEA). These nematodes infect a variety of soil-dwelling insects. Upon entering an insect host, the nematode releases Photorhabdus from its intestinal tract and the bacterium establishes a lethal septicemia in the insect.Rhabditoidea: A superfamily of nematodes of the order RHABDITIDA. Characteristics include an open tube stoma and an excretory system with lateral canals.Insects: The class Insecta, in the phylum ARTHROPODA, whose members are characterized by division into three parts: head, thorax, and abdomen. They are the dominant group of animals on earth; several hundred thousand different kinds having been described. Three orders, HEMIPTERA; DIPTERA; and SIPHONAPTERA; are of medical interest in that they cause disease in humans and animals. (From Borror et al., An Introduction to the Study of Insects, 4th ed, p1)Xenorhabdus: A genus of gram-negative, facultatively anaerobic rod-shaped cells which are motile by peritrichous flagella. Late in the growth cycle, spheroplasts or coccoid bodies occur, resulting from disintegration of the cell wall. The natural habitat is the intestinal lumen of certain nematodes. (From Bergey's Manual of Determinative Bacteriology, 9th ed)Nematoda: A class of unsegmented helminths with fundamental bilateral symmetry and secondary triradiate symmetry of the oral and esophageal structures. Many species are parasites.Moths: Insects of the suborder Heterocera of the order LEPIDOPTERA.Larva: Wormlike or grublike stage, following the egg in the life cycle of insects, worms, and other metamorphosing animals.Fatigue: The state of weariness following a period of exertion, mental or physical, characterized by a decreased capacity for work and reduced efficiency to respond to stimuli.Databases, Protein: Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.Sequence Analysis, Protein: A process that includes the determination of AMINO ACID SEQUENCE of a protein (or peptide, oligopeptide or peptide fragment) and the information analysis of the sequence.Systems Integration: The procedures involved in combining separately developed modules, components, or subsystems so that they work together as a complete system. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Internet: A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.Proteome: The protein complement of an organism coded for by its genome.Proteins: Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.Tuberculosis: Any of the infectious diseases of man and other animals caused by species of MYCOBACTERIUM.Tuberculosis, Pulmonary: MYCOBACTERIUM infections of the lung.Tuberculosis, Multidrug-Resistant: Tuberculosis resistant to chemotherapy with two or more ANTITUBERCULAR AGENTS, including at least ISONIAZID and RIFAMPICIN. The problem of resistance is particularly troublesome in tuberculous OPPORTUNISTIC INFECTIONS associated with HIV INFECTIONS. It requires the use of second line drugs which are more toxic than the first line regimens. TB with isolates that have developed further resistance to at least three of the six classes of second line drugs is defined as EXTENSIVELY DRUG-RESISTANT TUBERCULOSIS.Mycobacterium tuberculosis: A species of gram-positive, aerobic bacteria that produces TUBERCULOSIS in humans, other primates, CATTLE; DOGS; and some other animals which have contact with humans. Growth tends to be in serpentine, cordlike masses in which the bacilli show a parallel orientation.Extensively Drug-Resistant Tuberculosis: Tuberculosis resistant to ISONIAZID and RIFAMPIN and at least three of the six main classes of second-line drugs (AMINOGLYCOSIDES; polypeptide agents; FLUOROQUINOLONES; THIOAMIDES; CYCLOSERINE; and PARA-AMINOSALICYLIC ACID) as defined by the CDC.Medical History Taking: Acquiring information from a patient on past medical conditions and treatments.Drug Resistance, Multiple, Bacterial: The ability of bacteria to resist or to become tolerant to several structurally and functionally distinct drugs simultaneously. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).Lactobacillus brevis: A species of gram-positive, rod-shaped LACTIC ACID bacteria that is frequently used as starter culture in SILAGE fermentation, sourdough, and lactic-acid-fermented types of beer and wine.Lactobacillaceae: A family of gram-positive bacteria found regularly in the mouth and intestinal tract of man and other animals, in food and dairy products, and in fermenting vegetable juices. A few species are highly pathogenic.Bacillus megaterium: A species of bacteria whose spores vary from round to elongate. It is a common soil saprophyte.Xylulose: A 5-carbon keto sugar.Indole-3-Glycerol-Phosphate Synthase: An enzyme in the tryptophan biosynthetic pathway. EC 4.1.1.48.Anthranilate Synthase: An enzyme that catalyzes the formation of anthranilate (o-aminobenzoate) and pyruvic acid from chorismate and glutamine. Anthranilate is the biosynthetic precursor of tryptophan and numerous secondary metabolites, including inducible plant defense compounds. EC 4.1.3.27.AminohydrolasesAnthranilate Phosphoribosyltransferase: An enzyme that catalyzes the formation of N-5'-phosphoribosylanthranilic acid from anthranilate and phosphoribosylpyrophosphate, the first step in tryptophan synthesis in E. coli. It exists in a complex with ANTHRANILATE SYNTHASE in bacteria. EC 2.4.2.18.Thermotoga maritima: A rod-shaped bacterium surrounded by a sheath-like structure which protrudes balloon-like beyond the ends of the cell. It is thermophilic, with growth occurring at temperatures as high as 90 degrees C. It is isolated from geothermally heated marine sediments or hot springs. (From Bergey's Manual of Determinative Bacteriology, 9th ed)ortho-Aminobenzoates: Benzoic acids, salts, or esters that contain an amino group attached to carbon number 2 or 6 of the benzene ring structure.Neutrons: Electrically neutral elementary particles found in all atomic nuclei except light hydrogen; the mass is equal to that of the proton and electron combined and they are unstable when isolated from the nucleus, undergoing beta decay. Slow, thermal, epithermal, and fast neutrons refer to the energy levels with which the neutrons are ejected from heavier nuclei during their decay.Monosaccharides: Simple sugars, carbohydrates which cannot be decomposed by hydrolysis. They are colorless crystalline substances with a sweet taste and have the same general formula CnH2nOn. (From Dorland, 28th ed)Neutron Diffraction: The scattering of NEUTRONS by matter, especially crystals, with accompanying variation in intensity due to interference effects. It is useful in CRYSTALLOGRAPHY and POWDER DIFFRACTION.Arthrobacter: A genus of asporogenous bacteria isolated from soil that displays a distinctive rod-coccus growth cycle.

Cobalt proteins. (1/391)

In the form of vitamin B12, cobalt plays a number of crucial roles in many biological functions. However, recent studies have provided information on the biochemistry and bioinorganic chemistry of several proteins containing cobalt in a form other than that in the corrin ring of vitamin B12. To date, eight noncorrin-cobalt-containing enzymes (methionine aminopeptidase, prolidase, nitrile hydratase, glucose isomerase, methylmalonyl-CoA carboxytransferase, aldehyde decarbonylase, lysine-2,3-aminomutase, and bromoperoxidase) have been isolated and characterized. A cobalt transporter is involved in the metallocenter biosynthesis of the host cobalt-containing enzyme, nitrile hydratase. Understanding the differences between cobalt and nickel transporters might lead to drug development for gastritis and peptic ulceration.  (+info)

Construction and characterization of Escherichia coli disruptants defective in the yaeM gene. (2/391)

Escherichia coli disruptants defective in the yaeM gene, which is located at 4.2 min on the chromosome map, were constructed and characterized. The disruptants showed auxotrophy for 2-C-methylerythritol, a free alcohol of 2-C-methyl-D-erythritol 4-phosphate that is a biosynthetic precursor in the nonmevalonate pathway. This result clearly shows that the yaeM gene is indeed involved in this pathway in E. coli.  (+info)

Efficient expression, purification and crystallisation of two hyperthermostable enzymes of histidine biosynthesis. (3/391)

Enzymes from hyperthermophiles can be efficiently purified after expression in mesophilic hosts and are well-suited for crystallisation attempts. Two enzymes of histidine biosynthesis from Thermotoga maritima, N'-((5'-phosphoribosyl)-formimino)-5-aminoimidazol-4-carb oxamid ribonucleotide isomerase and the cyclase moiety of imidazoleglycerol phosphate synthase, were overexpressed in Escherichia coli, both in their native and seleno-methionine-labelled forms, purified by heat precipitation of host proteins and crystallised. N'-((5'-phosphoribosyl)-formimino)-5-aminoimidazol-4-carb oxamid ribonucleotide isomerase crystallised in four different forms, all suitable for X-ray structure solution, and the cyclase moiety of imidazoleglycerol phosphate synthase yielded one crystal form that diffracted to atomic resolution. The obtained crystals will enable the determination of the first three-dimensional structures of enzymes from the histidine biosynthetic pathway.  (+info)

Cloning and heterologous expression of a cDNA encoding 1-deoxy-D-xylulose-5-phosphate reductoisomerase of Arabidopsis thaliana. (4/391)

Various plant isoprenoids are synthesized via the non-mevalonate pathway of isopentenyl diphosphate formation. In this pathway, 1-deoxy-D-xylulose 5-phosphate (DOXP), the first intermediate, is transformed to 2-C-methyl-D-erythritol 4-phosphate (MEP) by an enzyme which was recently cloned from Escherichia coli. In order to find a plant homologue of this 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) we cloned a cDNA fragment from Arabidopsis thaliana which has high homology to the E. coli DXR. By expression of this fragment in E. coli we could demonstrate that it encodes a protein which transforms DOXP to MEP. The antibiotic fosmidomycin specifically inhibits this DXR enzyme activity.  (+info)

A molecular sensor that allows a gut commensal to control its nutrient foundation in a competitive ecosystem. (5/391)

Little is known about how members of the indigenous microflora interact with their mammalian hosts to establish mutually beneficial relationships. We have used a gnotobiotic mouse model to show that Bacteroides thetaiotaomicron, a component of the intestinal microflora of mice and humans, uses a repressor, FucR, as a molecular sensor of L-fucose availability. FucR coordinates expression of an operon encoding enzymes in the L-fucose metabolic pathway with expression of another locus that regulates production of fucosylated glycans in intestinal enterocytes. Genetic and biochemical studies indicate that FucR does this by using fucose as an inducer at one locus and as a corepressor at the other locus. Coordinating this commensal's immediate nutritional requirements with production of a host-derived energy source is consistent with its need to enter and persist within a competitive ecosystem.  (+info)

Gene silencing: Maintaining methylation patterns. (6/391)

Recent studies of an Arabidopsis gene family have shown that inverted repeats can be potent silencers of other identical sequences in the genome, causing them to become stably methylated at cytosine residues. From mutations affecting this process we are beginning to understand how methylation patterns are maintained.  (+info)

Increasing the thermostability of D-xylose isomerase by introduction of a proline into the turn of a random coil. (7/391)

Thermostability can be increased by introducing prolines at suitable sites in target proteins. Two single (G138P, G247D) mutants and one double (G138P/G247D) mutant of xylose isomerase from Streptomyces diastaticus No.7, strain M1033 have been constructed by site-directed mutagenesis. With respect to the wild-type enzyme, G138P showed about a 100% increase in thermostability, and G247D showed an increased catalytic activity. Significantly, the double mutant, G138P/G247D displayed even higher activity than G247D and better heat stability than G138P. Its half life was about 2.5-fold greater than the wild-type enzyme, using xylose as a substrate. Molecular modelling suggested that the introduction of a proline residue in the turn of a random coil may cause the surrounding conformation to be tightened by reducing the backbone flexibility. The change in thermostability can, therefore, be explained based on changes in the molecular rigidity. Furthermore, the improvements in the properties of the double mutant indicated that the advantages of two single mutants can be combined effectively.  (+info)

Arabidopsis PAI gene arrangements, cytosine methylation and expression. (8/391)

Previous analysis of the PAI tryptophan biosynthetic gene family in Arabidopsis thaliana revealed that the Wassilewskija (WS) ecotype has four PAI genes at three unlinked sites: a tail-to-tail inverted repeat at one locus (PAI1-PAI4) plus singlet genes at two other loci (PAI2 and PAI3). The four WS PAI genes are densely cytosine methylated over their regions of DNA identity. In contrast, the Columbia (Col) ecotype has three singlet PAI genes at the analogous loci (PAI1, PAI2, and PAI3) and no cytosine methylation. To understand the mechanism of PAI gene duplication at the polymorphic PAI1 locus, and to investigate the relationship between PAI gene arrangement and PAI gene methylation, we analyzed 39 additional ecotypes of Arabidopsis. Six ecotypes had PAI arrangements similar to WS, with an inverted repeat and dense PAI methylation. All other ecotypes had PAI arrangements similar to Col, with no PAI methylation. The novel PAI-methylated ecotypes provide insights into the mechanisms underlying PAI gene duplication and methylation, as well as the relationship between methylation and gene expression.  (+info)

1PII: Three-dimensional structure of the bifunctional enzyme phosphoribosylanthranilate isomerase: indoleglycerolphosphate synthase from Escherichia coli refined at 2.0 A resolution.
Background. The GNPDA2 (glucosamine-6-phosphate deaminase 2) gene is a member of Glucosamine-6-phosphate (GlcN6P) deaminase subfamily, which encoded an allosteric enzyme of GlcN6P. Genome-wide association studies (GWAS) have shown that variations of human GNPDA2 are associated with body mass index and obesity risk, but its function and metabolic implications remain to be elucidated.The object of this study was to characterize the gene structure, expression, and biological functions of GNPDA2 in chickens. Methods. Variant transcripts of chicken GNPDA2 and their expression were investigated using rapid amplification of cDNA ends (RACE) system and real-time quantitative PCR technology. We detected the GNPDA2 expression in hypothalamic, adipose, and liver tissue of Xinghua chickens with fasting and high-glucose-fat diet treatments, and performed association analysis of variations of GNPDA2 with productive traits in chicken. The function of GNPDA2 was further studied by overexpression and small interfering
In order to overproduce D-xylose isomerase, the Escherichia coli D-xylose isomerase (D-xylose ketol-isomerase, EC 5.3.1.5) gene (xylA) was fused to ${\lambda}P_{L}$ promoter. The promoterless xylA gene containing the ribosome binding site and coding region for D-xylose isomerase was cloned into a ...
In enzymology, a xylose isomerase (EC 5.3.1.5) is an enzyme that catalyzes the interconversion of D-xylose and D-xylulose. This enzyme belongs to the family of isomerases, specifically those intramolecular oxidoreductases interconverting aldoses and ketoses. The isomerase has now been observed in nearly a hundred species of bacteria. Xylose-isomerases are also commonly called fructose-isomerases due to their ability to interconvert glucose and fructose. The systematic name of this enzyme class is D-xylose aldose-ketose-isomerase. Other names in common use include D-xylose isomerase, D-xylose ketoisomerase, and D-xylose ketol-isomerase. The activity of D-xylose isomerase was first observed by Mitsuhashi and Lampen in 1953 in the bacterium Lactobacillus pentosus. Artificial production through transformed E.coli have also been successful. In 1957, the D-xylose isomerase activity on D-glucose conversion to D-fructose was noted by Kooi and Marshall. It is now known that isomerases have broad ...
casSAR Dugability of P25170 | TRPC | Multifunctional tryptophan biosynthesis protein - Also known as TRPG_PHACH, TRPC. Trifunctional enzyme bearing the Gln amidotransferase (GATase) domain of anthranilate synthase, indole-glycerolphosphate synthase, and phosphoribosylanthranilate isomerase activities.
anthranilate synthase / indole-3-glycerol phosphate synthase / phosphoribosylanthranilate isomerase [EC:4.1.3.27 4.1.1.48 5.3.1.24 ...
Keywords: Keywords: DNA methylation - Gene silencing - RNA-directed DNA methylation - RNA interference - Transposon.; Abbreviations: Ac, Activator; BAL, BALL; CMT, CHROMOMETHYLASE; DDM, DECREASE IN DNA METHYLATION; DRM, DOMAIN REARRANGED METHYLTRANSFERASE; En/Spm, Enhancer/Suppressor-mutator; MET, METHYLTRANSFERASE; MOP, MODIFIER OF PARAMUTATION; PAI, PHOSPHORIBOSYLANTHRANILATE ISOMERASE; PTGS, post-transcriptional gene silencing; RdDM, RNA-directed DNA methylation; RNAi, RNA interference; SUP, SUPERMAN; TGS, transcriptional gene silencing; TSI, transcriptionally silent information. Journal Article. 5263 words. Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry ...
1K5H: Crystal structure of 1-deoxy-D-xylulose-5-phosphate reductoisomerase, a crucial enzyme in the non-mevalonate pathway of isoprenoid biosynthesis.
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
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Perform reliable qPCR with Bio-Rads pre-validated GNPDA2 primer pair, for the Chicken genome. Designed for SYBR Green-based detection.
GNPDA1 Antibody 12312-1-AP has been identified with IHC, WB, ELISA. 12312-1-AP detected 33 kDa band in K-562 cells with 1:500-1:2000 dilution...
GNPDA2 Antibody 17105-1-AP has been identified with IHC, WB, ELISA. 17105-1-AP detected 31 kDa band in rat kidney tissue with 1:500-1:1000 dilution...
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Note from Pfam: this protein didnt match Pfam, but it pretty soon became clear that this protein should have belonged to PF01182. I have rebuilt that family to include this protein. Gene YP_001050605 from SHEWANELLA BALTICA OS155 encodes a protein with 232 residues, whcih has annotated 6-phosphogluconolactonase. Sequence alignment indicates that this proteim belongs to the sugarP_isomerase superfamily. Dali search even suggests this target be a glucose-6-phosphate 1-dehydrogenase or glucosamine-6-phosphate deaminase. More details for this structures investigation will be presented in the future hopefully. ...
MetabolismCentral intermediary metabolismAmino sugarsglucosamine-6-phosphate deaminase (TIGR00502; EC 3.5.99.6; HMM-score: 33.2) ...
D-xylose isomerase (XI) is capable of sugar isomerization and slow conversion of some monosaccharides into their C2-epimers. We present X-ray and neutron ...
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MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOther1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 20.4) ...
Isomerases are a broad class of enzymes which catalyze structural change in a single molecule in biological systems. These structural changes can be ext...
Phosphoribosylanthranilate isomerase and indoleglycerol-phosphate synthase: tryptophan biosynthetic enzymes from Thermotoga maritima ...
TY - JOUR. T1 - A thermostable xylose isomerase from Thermus caldophilus. T2 - Biochemical characterization, crystallization and preliminary x-ray analysis. AU - Chang, Changsoo. AU - Song, Hyun Kyu. AU - Park, Byung Chul. AU - Lee, Dae Sil. AU - Suh, Se Won. PY - 1999/1/1. Y1 - 1999/1/1. N2 - A highly thermostable xylose isomerase from Thermus caldophilus has been expressed in Escherichia coli. The purified enzyme has an optimum temperature of 363 K. It has been crystallized at room temperature using ammonium sulfate as a precipitant. The crystal belongs to the orthorhombic space group P212121, with unit-cell parameters a = 84.35, b = 123.60, c = 140.24 Å. The presence of one molecule of tetrameric xylose isomerase in the asymmetric unit gives a crystal volume per protein mass (V(m)) of 2.1 Å D-1 and a solvent content of 41% by volume. The crystals initially showed diffraction to 1.7 Å Bragg spacing with synchrotron X-rays, and a set of native data extending to 2.3 Å resolution has been ...
292123913 - EP 1095153 A1 2001-05-02 - BIOLOGICAL TAGATOSE PRODUCTION BY RECOMBINANT ESCHERICHIA COLI - [origin: WO0068397A1] This invention relates to a recombinant i Escherichia coli /i and a process for producing D-tagatose. In detail, it includes the construction of recombinant i E.coli /i harboring L-arabinose isomerase, whole-cell conversion of D-galactose into D-tagatose by recombinant i E.coli /i expressing L-arabinose isomerase, enzymatic production of D-tagatose by the extract of recombinant i E.coli /i expressing L-arabinose isomerase, and bioconversion by immobilized L-arabinose isomerase.[origin: WO0068397A1] This invention relates to a recombinant i Escherichia coli /i and a process for producing D-tagatose. In detail, it includes the construction of recombinant i E.coli /i harboring L-arabinose isomerase, whole-cell conversion of D-galactose into D-tagatose by recombinant i E.coli /i expressing L-arabinose isomerase, enzymatic production of D-tagatose by the extract of
Background L-arabinose isomerase (AI) is a crucial catalyst for the biotransformation of D-galactose to D-tagatose. In previous reports, AIs from thermophilic bacterial strains had been wildly...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Tuberculosis, caused by the pathogenic bacteria Mycobacterium tuberculosis, is one of the most widespread and deadly infectious diseases today. Treatment of tuberculosis relies on antibiotics that were developed more than 50 years ago. These are now becoming ineffective due to the emergence of antibiotic resistant strains of the bacteria.. The aim of the research in this thesis was to develop new antibiotics for tuberculosis treatment. To this end, we targeted enzymes from two essential biosynthetic pathways in M. tuberculosis for drug development. The methylerythritol phosphate (MEP) pathway synthesizes a group of compounds called isoprenoids. These compounds have essential roles in all living organisms. The fact that humans utilize a different pathway for isoprenoid synthesis makes the MEP pathway enzymes attractive targets for drug development. We have determined the structures of two essential enzymes from this pathway by X-ray crystallography: 1-deoxy-D-xylulose 5-phosphate reductoisomerase ...
SWISS-MODEL Repository entry for C4L656 (DXR_EXISA), 1-deoxy-D-xylulose 5-phosphate reductoisomerase. Exiguobacterium sp (strain ATCC BAA-1283 / AT1b)
Glucose-6-phosphate isomerase (GPI) (EC 5.3.1.9) or phosphoglucose isomerase (PGI) [1,2] is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. PGI is involved in different pathways: in most higher organisms it is involved in glycolysis; in mammals it is involved in gluconeogenesis; in plants in carbohydrate biosynthesis; in some bacteria it provides a gateway for fructose into the Entner-Doudouroff pathway. Besides its role as a glycolytic enzyme, mammalian PGI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. Mammalian PGI is also neuroleukin [3], a neurotrophic factor which supports the survival of various types of neurons. The sequence of PGI is conserved among diverse species ranging from bacteria to mammals and structures form a similar fold (see ,PDB:1IAT,) [4,5], comprised of two subdomains that each form an α-β-α sandwich, with the active site located in the ...
Phosphomannose isomerase is a zinc binding enzyme that catalyses the reversible isomerization of mannose 6-phosphate and fructose 6-phosphate. These substrates could exist in two conformations. They are covalently closed (cyclic form) in one conformation while a covalent bond is disrupted in the other linear form. The reaction most likely proceeds by binding of the cyclic form of substrate, conversion of its closed to open form, transfer of protons between atoms of the open form of substrate by a suitable base followed by its cyclisation to form the cyclic form of the product. Structure of phosphomannose isomerase from Salmonella typhimurium The ...
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8xia: X-ray analysis of D-xylose isomerase at 1.9 A: native enzyme in complex with substrate and with a mechanism-designed inactivator.
Ll_KARI is a representative of the 14% of the 643 KARI sequences with a six-residue β2αB loop. Whereas in 12- and seven-residue β2αB-loop KARIs the antepenultimate and ultimate residues are highly conserved, in six-loop KARIs the ultimate conserved serine is usually preceded by a positively charged residue at the penultimate position. We hypothesized that residues Lys52 and Ser53 of Ll_KARI were equivalent in function to residues Arg76 and Ser78 in Ec_IlvC. Ll_KARIDD variant with mutations Lys52Asp and Ser53Asp, however, expressed at an extremely low level and exhibited no measurable activity with either cofactor, which could be a result of the destabilizing effects of the two adjacent Asp residues. Similar results were obtained with Ll_KARIED. These two Asp mutations are separated by an additional residue in KARIs with the longer β2αB loops. The failed transfer of E. coli cofactor switch mutations to Ll_KARI suggests that KARIs with six-residue loops require a modified approach.. To ...
van Tilbeurgh, H., J. Jenkins, M. Chiadmi, J. Janin, S. J. Wodak, N. T. Mrabet, and A. M. Lambeir, Protein engineering of xylose (glucose) isomerase from Actinoplanes missouriensis. 3. Changing metal specificity and the pH profile by site-directed mutagenesis., Biochemistry, vol. 31, issue 24, pp. 5467-71, 1992 Jun 23. ...
van Tilbeurgh, H., J. Jenkins, M. Chiadmi, J. Janin, S. J. Wodak, N. T. Mrabet, and A. M. Lambeir, Protein engineering of xylose (glucose) isomerase from Actinoplanes missouriensis. 3. Changing metal specificity and the pH profile by site-directed mutagenesis., Biochemistry, vol. 31, issue 24, pp. 5467-71, 1992 Jun 23. ...
RPI TRIGGER WHEEL :: Soothe sore muscles with the surprisingly simple and extremely effective recovery device, the RPI Trigger Wheel. Get quick relief from workout pain, stiffness and soreness with this hi-tech tool that features proprietary Intracell technology designed by physicians and endorsed by hospitals, clinics and institutions. Youll love the convenience of easily carrying it anywhere, because the RPI Trigger Wheels compact design easily fits into your workout bag or pocket of your running shorts. ...
Not yet tested, comments welcome Project files for Eagle here: [4] Schematic and Board images: [5] [6] This board uses Microchip 16-pin GPIO expanders driven by SPI to provide up to 256 additional GPIO pins at a very low cost. Optionally, it can supply power for the board and the Raspberry PI. The board can be made in a stackable manner allowing you to also place additional peripherals on the GPIO connector. It makes non-exclusive use of all four SPI pins as well as exclusive use of the CE0 Pin. If you install (and power) the power supply components, the board will supply up to 1A at 5VDC. This is not enough to power all GPIO pins on a fully populated board, but in most applications should be sufficient to power the RPI and a reasonable number of GPIOs. An external power supply should be used for higher-power applications. This board should never be powered from the RPI as even one of the chips can draw more than the RPI can provide. The power supply is quite flexible and will accept anything ...
Several hits in gapped BLAST to lacA-related proteins, e.g. residues 4-150 are 43% similar to the enzyme from M.tuberculosis. Residues 5-151 are 40% similar to MG396, a predicted lacA/rpiB galactoside acetyltransferase. Other similarities involve hypothetical proteins and predicted phosphoriboisomerase B proteins (rpiB), e.g. residues 2-153 are 36% similar to RPIB_ECOLI. No significant similarities to T.pallidum or C.trachomatis ...
... - For customers seeking comprehensive skeletal support Contributes to the normal functioning of the bones and muscles Convenient EasyGest capsules for faster digestion Manufactured in the UK to strict GMP standards
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
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Substance Name:Phosphate Buffered Saline 1X Sterile Solution. CAS Number:7647-14-5, 7447-40-7, 7558-79-4, 7778-77-0, 7732-18-5. ...
Any chance they could raise the stage so that the band would be above the glass? That would pump up the volume on the ice and bring back the intimidation factor. And the band members would be protected by the netting ...
Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2543 DE 01-10-2019 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013. ...
Saccharomyces cerevisiae strains expressing D-xylose isomerase (XI) produce some of the highest reported ethanol yields from D-xylose. Unfortunately, most bacterial XIs that have been expressed in S. cerevisiae are either not functional, require additional strain modification, or have low affinity for D-xylose. This study analyzed several XIs from rumen and intestinal microorganisms to identify enzymes with improved properties for engineering S. cerevisiae for D-xylose fermentation. Four XIs originating from rumen and intestinal bacteria were isolated and expressed in a S. cerevisiae CEN.PK2-1C parental strain primed for D-xylose metabolism by over expression of its native D-xylulokinase. Three of the XIs were functional in S. cerevisiae, based on the strains ability to grow in D-xylose medium. The most promising strain, expressing the XI mined from Prevotella ruminicola TC2-24, was further adapted for aerobic and fermentative growth by serial transfers of D-xylose cultures under aerobic, and followed
Fosmidomycin is a phosphonic antibiotic which inhibits 1-deoxy-D-xylulose 5-phosphate reductoisomerase (Dxr), the first committed step of the non-mevalonate pathway of isoprenoid biosynthesis. In Mycobacterium tuberculosis Dxr is encoded by Rv2870c, and although the antibiotic has been shown to inhibit the recombinant enzyme [1], mycobacteria are intrinsically resistant to fosmidomycin at the whole cell level. Fosmidomycin is a hydrophilic molecule and in many bacteria its uptake is an active process involving a cAMP dependent glycerol-3-phosphate transporter (GlpT). The fact that there is no glpT homologue in the M. tuberculosis genome and the highly impervious nature of the hydrophobic mycobacterial cell wall suggests that resistance may be due to a lack of cellular penetration. We demonstrated that dxr (Rv2780c) is an essential gene in M. tuberculosis, since we could not delete the chromosomal copy unless a second functional copy was provided on an integrating vector. This confirmed that the
ribose-5-phosphate isomerage B (RpiB):Presented here is a series of crystal structures solved by the Seattle Structural Genomics Center for Infectious Disease (SSGCID) of ribose-5-phosphate isomerase B, or RpiB, from the pathogenic fungus, Coccidioides immitis. This parasite, which resides in the soil in certain parts of the western hemisphere, causes coccidioidomycosis, also known as Valley Fever. The disease is difficult to diagnose as it causes masses which mimics a lung tumor. Ribose-5-phosphate isomerase is an enzyme that catalyzes the conversion between ribose-5-phosphate and ribulose-5-phosphate. This family of enzymes naturally occurs in two distinct classes, RpiA and RpiB, which play, among others, an important role in the pentose phosphate pathway, which converts a type of glucose into other molecules. Although RpiB occurs predominantly in bacteria, the RpiB from this fungal pathogen contains high structural similarity to other known RpiB structures despite modest sequence similarity. The C.
... aldose-ketose isomerases MeSH D08.811.399.475.200.174 --- autocrine motility factor MeSH D08.811.399.475.200.350 --- glucose-6- ... triose-phosphate isomerase MeSH D08.811.399.475.400 --- carbon-carbon double bond isomerases MeSH D08.811.399.475.400.700 --- ... steroid isomerases MeSH D08.811.399.475.800 --- sulfur-sulfur bond isomerases MeSH D08.811.399.475.800.550 --- protein ... phosphate isomerase MeSH D08.811.399.475.200.550 --- mannose-6-phosphate isomerase MeSH D08.811.399.475.200.662 --- neuroleukin ...
Like most sugar isomerases, glucose isomerase catalyzes the interconversion of aldoses and ketoses. The conversion of glucose ... Phosphohexose Isomerase Dificiency (PHI) is also known as phosphoglucose isomerase deficiency or Glucose-6-phosphate isomerase ... Generally, "the names of isomerases are formed as "substrate isomerase" (for example, enoyl CoA isomerase), or as "substrate ... A ketose is then formed and the ring is closed again. Glucose-6-phosphate first binds to the active site of the isomerase. The ...
The systematic name of this enzyme class is D-arabinose aldose-ketose-isomerase. Other names in common use include D-arabinose( ... L-fucose) isomerase, D-arabinose isomerase, L-fucose isomerase, and D-arabinose ketol-isomerase. As of late 2007, only one ... This enzyme belongs to the family of isomerases, specifically those intramolecular oxidoreductases interconverting aldoses and ... In enzymology, an arabinose isomerase (EC 5.3.1.3) is an enzyme that catalyzes the chemical reaction D-arabinose ⇌ {\ ...
The systematic name of this enzyme class is hydroxypyruvate aldose-ketose-isomerase. This enzyme participates in glyoxylate and ... This enzyme belongs to the family of isomerases, specifically those intramolecular oxidoreductases interconverting aldoses and ... de Windt FE, van der Drift C (1980). "Purification and some properties of hydroxypyruvate isomerase of Bacillus fastidiosus". ... In enzymology, a hydroxypyruvate isomerase (EC 5.3.1.22) is an enzyme that catalyzes the chemical reaction hydroxypyruvate ⇌ {\ ...
... anthranilate aldose-ketose-isomerase. Other names in common use include: PRA isomerase, PRAI, IGPS:PRAI (indole-3-glycerol- ... As the name phosphoribosylanthranilate isomerase suggests, it functions as an isomerase, rearranging the parts of the molecule ... specifically those intramolecular oxidoreductases interconverting aldoses and ketoses. The systematic name of this enzyme class ... anthranilate ketol-isomerase. Phosphoribosylanthranilate isomerase (PRAI) is monomeric and labile in most mesophilic ...
The systematic name of this enzyme class is D-glucuronate aldose-ketose-isomerase. Other names in common use include uronic ... isomerase, uronate isomerase, D-glucuronate isomerase, uronic acid isomerase, and D-glucuronate ketol-isomerase. This enzyme ... This enzyme belongs to the family of isomerases, specifically those intramolecular oxidoreductases interconverting aldoses and ... In enzymology, a glucuronate isomerase (EC 5.3.1.12) is an enzyme that catalyzes the chemical reaction D-glucuronate ⇌ {\ ...
The systematic name of this enzyme class is D-xylose aldose-ketose-isomerase. Other names in common use include D-xylose ... specifically those intramolecular oxidoreductases interconverting aldoses and ketoses. The isomerase has now been observed in ... Xylose-isomerases are also commonly called fructose-isomerases due to their ability to interconvert glucose and fructose. ... isomerase, D-xylose ketoisomerase, and D-xylose ketol-isomerase. The activity of D-xylose isomerase was first observed by ...
The systematic name of this enzyme class is D-ribose aldose-ketose-isomerase. Other names in common use include D-ribose ... This enzyme belongs to the family of isomerases, specifically those intramolecular oxidoreductases interconverting aldoses and ... In enzymology, a ribose isomerase (EC 5.3.1.20) is an enzyme that catalyzes the chemical reaction D-ribose ⇌ {\displaystyle \ ... Izumori K, Rees AW, Elbein AD (1975). "Purification, crystallization, and properties of D-ribose isomerase from Mycobacterium ...
The systematic name of this enzyme class is D-mannose aldose-ketose-isomerase. Other names in common use include D-mannose ... This enzyme belongs to the family of isomerases, specifically those intramolecular oxidoreductases interconverting aldoses and ... In enzymology, a mannose isomerase (EC 5.3.1.7) is an enzyme that catalyzes the chemical reaction D-mannose ⇌ {\displaystyle \ ... Palleroni NJ; Doudoroff M (1956). "Mannose isomerase of Pseudomonas saccharophila". J. Biol. Chem. 218: 535-548. PMID 13278359 ...
... is an enzyme with systematic name D-glycero-D-manno-heptose 7-phosphate aldose-ketose-isomerase. This enzyme catalyses the ... D-sedoheptulose 7-phosphate isomerase (EC 5.3.1.28, sedoheptulose-7-phosphate isomerase, phosphoheptose isomerase, gmhA (gene ... Kim, M.S.; Shin, D.H. (2009). "A preliminary X-ray study of sedoheptulose-7-phosphate isomerase from Burkholderia pseudomallei ... D-sedoheptulose 7-phosphate isomerase at the US National Library of Medicine Medical Subject Headings (MeSH) Molecular and ...
The systematic name of this enzyme class is L-fucose aldose-ketose-isomerase. This enzyme participates in fructose and mannose ... This enzyme belongs to the family of isomerases, specifically those intramolecular oxidoreductases interconverting aldoses and ... In enzymology, a L-fucose isomerase (EC 5.3.1.25) is an enzyme that catalyzes the chemical reaction L-fucose ⇌ {\displaystyle \ ... The enzyme is a hexamer, forming the largest structurally known ketol isomerase, and has no sequence or structural similarity ...
The systematic name of this enzyme class is L-arabinose aldose-ketose-isomerase. This enzyme participates in pentose and ... This enzyme belongs to the family of isomerases, specifically those intramolecular oxidoreductases interconverting aldoses and ... L-arabinose isomerase". J. Biol. Chem. 231 (2): 1031-7. PMID 13539034. Nakamatu T, Yamanaka K (1969). "Crystallization and ... In enzymology, a L-arabinose isomerase (EC 5.3.1.4) is an enzyme that catalyzes the chemical reaction L-arabinose ⇌ {\ ...
The systematic name of this enzyme class is L-rhamnose aldose-ketose-isomerase. Other names in common use include rhamnose ... This enzyme belongs to the family of isomerases, specifically those intramolecular oxidoreductases interconverting aldoses and ... I L-RHAMNOSE ISOMERASE FROM LACTOBACILLUS PLANTARUM.]". Biochem. Z. 339: 145-53. PMID 14095156. Molecular and Cellular Biology ... In enzymology, a L-rhamnose isomerase (EC 5.3.1.14) is an enzyme that catalyzes the chemical reaction L-rhamnose ⇌ {\ ...
The systematic name of this enzyme class is 11-deoxycorticosterone aldose-ketose-isomerase. Martin KO, Oh SW, Lee HJ, Monder C ... This enzyme belongs to the family of isomerases, specifically those intramolecular oxidoreductases interconverting aldoses and ... In enzymology, a corticosteroid side-chain-isomerase (EC 5.3.1.21) is an enzyme that catalyzes the chemical reaction 11- ... Monder C, Martin KO, Bogumil J (1980). "Presence of epimerase activity in hamster liver corticosteroid side chain isomerase". J ...
The systematic name of this enzyme class is D-galactose-6-phosphate aldose-ketose-isomerase. This enzyme participates in ... This enzyme belongs to the family of isomerases, specifically those intramolecular oxidoreductases interconverting aldoses and ... In enzymology, a galactose-6-phosphate isomerase (EC 5.3.1.26) is an enzyme that catalyzes the chemical reaction D-galactose 6- ...
The systematic name of this enzyme class is D-arabinose-5-phosphate aldose-ketose-isomerase. Other names in common use include ... This enzyme belongs to the family of isomerases, specifically those intramolecular oxidoreductases interconverting aldoses and ... In enzymology, an arabinose-5-phosphate isomerase (EC 5.3.1.13) is an enzyme that catalyzes the chemical reaction D-arabinose 5 ... arabinose phosphate isomerase, phosphoarabinoisomerase, and D-arabinose-5-phosphate ketol-isomerase. Volk WA (1960). " ...
The systematic name of this enzyme class is D-lyxose aldose-ketose-isomerase. Other names in common use include D-lyxose ... This enzyme belongs to the family of isomerases, specifically those intramolecular oxidoreductases interconverting aldoses and ... In enzymology, a D-lyxose ketol-isomerase (EC 5.3.1.15) is an enzyme that catalyzes the chemical reaction D-lyxose ⇌ {\ ... ANDERSON RL, ALLISON DP (1965). "PURIFICATION AND CHARACTERIZATION OF D-LYXOSE ISOMERASE". J. Biol. Chem. 240: 2367-72. PMID ...
The systematic name of this enzyme class is D-ribose-5-phosphate aldose-ketose-isomerase. RpiA in human beings is encoded on ... Ribose-5-phosphate isomerase deficiency is mutated in a rare disorder, Ribose-5-phosphate isomerase deficiency. The disease has ... Ribose-5-phosphate isomerase (Rpi) is an enzyme that catalyzes the conversion between ribose-5-phosphate (R5P) and ribulose-5- ... The first is an insertion of a premature stop codon into the gene encoding the isomerase, and the second is a missense mutation ...
MPI must convert an aldose (mannose) to a ketose (fructose), in addition to opening and closing the rings for these sugars. In ... Mannose-6 phosphate isomerase (MPI), alternately phosphomannose isomerase (PMI) (EC 5.3.1.8) is an enzyme which facilitates the ... Anomeric Form used by Phosphomannose Isomerase and Its 1-Epimerization by Phosphoglucose Isomerase". The Journal of Biological ... "Structural Basis for Phosphomannose Isomerase Activity in Phosphoglucose Isomerase from Pyrobaculum Aerophilum: A Subtle ...
... aldose-ketose-isomerase, 1-phospho-5'-S-methylthioribose isomerase, and S-methyl-5-thio-D-ribose-1-phosphate aldose-ketose- ... The systematic name of this enzyme class is S-methyl-5-thio-alpha-D-ribose-1-phosphate aldose-ketose-isomerase. Other names in ... This enzyme belongs to the family of isomerases, specifically those intramolecular oxidoreductases interconverting aldoses and ... 1-PMTR isomerase, 5-methylthio-5-deoxy-D-ribose-1-phosphate ketol-isomerase, S-methyl-5-thio-5-deoxy-D-ribose-1-phosphate ketol ...
... imidazole-4-carboxamide aldose-ketose-isomerase. This enzyme catalyses the following chemical reaction 1-(5-phosphoribosyl)-5 ... 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16, N-(5'-phospho-D ... 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase at the US National Library of ... isomerase) of histidine biosynthesis from Salmonella typhimurium". J. Biol. Chem. 241: 3262-3269. PMID 5330429. ...
The systematic name of this enzyme class is 4-deoxy-L-threo-5-hexosulose-uronate aldose-ketose-isomerase. This enzyme is also ... This enzyme belongs to the family of isomerases, specifically those intramolecular oxidoreductases interconverting aldoses and ... In enzymology, a 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase (EC 5.3.1.17) is an enzyme that catalyzes the chemical ... Preiss J (1966). "4-Deoxy-L-threo-5-hexosulose uronic acid isomerase". Carbohydrate Metabolism. Methods in Enzymology. 9. pp. ...
The mechanism that GPI uses to interconvert glucose 6-phosphate and fructose 6-phosphate (aldose to ketose) consists of three ... Glucose-6-phosphate isomerase (GPI), alternatively known as phosphoglucose isomerase (PGI) or phosphohexose isomerase (PHI), is ... Glucose-6-phosphate isomerase in PROSITE Phosphoglucose Isomerase Glucose phosphate isomerase deficiency This article ... Swan MK, Hansen T, Schonheit P, Davies C (September 2004). "A novel phosphoglucose isomerase (PGI)/phosphomannose isomerase ...
Meyerhoff and Junowicz found that the equilibrium constant for the isomerase and aldoses reaction were not affected by ... a ketose), and glyceraldehyde 3-phosphate (an aldose). There are two classes of aldolases: class I aldolases, present in ... G6P is then rearranged into fructose 6-phosphate (F6P) by glucose phosphate isomerase. Fructose can also enter the glycolytic ... Triosephosphate isomerase rapidly interconverts dihydroxyacetone phosphate with glyceraldehyde 3-phosphate (GADP) that proceeds ...
Meyerhoff and Junowicz found that the equilibrium constant for the isomerase and aldoses reaction were not affected by ... a ketose), and glyceraldehyde 3-phosphate (an aldose). There are two classes of aldolases: class I aldolases, present in ... Cofactors: Mg2+ G6P is then rearranged into fructose 6-phosphate (F6P) by glucose phosphate isomerase. Fructose can also enter ... The reaction requires an enzyme, phosphohexose isomerase, to proceed. This reaction is freely reversible under normal cell ...
Aldose · Ketose · Furanose · Pyranose. Geometry. Cyclohexane conformation · Anomer · Mutarotation. Monosaccharides. Dioses. ... Glucose-6-phosphate isomerase ഫ്രക്ടോസ് 6-ഫോസ്ഫേറ്റ് phosphofructokinase-1 Fructose 1,6-bisphosphate Fructose-bisphosphate ... Triosephosphate isomerase Glyceraldehyde 3-phosphate Glyceraldehyde-3-phosphate dehydrogenase 1,3-Bisphosphoglycerate ...
Aldose-Ketose Isomerases); EC 5.3.1.12 (glucuronate isomerase); SY7Q814VUP (Calcium). ... Aldose-Ketose Isomerases); EC 5.3.1.12 (glucuronate isomerase). ... Aldose-Cetose Isomerases/qu mica. Bioqu mica/m todos. ... Aldose-Cetose Isomerases. Escherichia coli/gen tica. Regula o da Express o G nica. Genes Reguladores. cidos Hexur nicos/ ... The isomerase was found to be inhibited by EDTA and to be stimulated by Zn(2+), Co(2+), and Mn(2+), but not by Mg(2+) or Ca(2+ ...
Putative tagatose-6-phosphate ketose/aldose isomerase 4.1. 0.001. SpnNT_00089. agaC_1 ...
The systematic name of this enzyme class is D-xylose aldose-ketose-isomerase. Other names in common use include D-xylose ... specifically those intramolecular oxidoreductases interconverting aldoses and ketoses. The isomerase has now been observed in ... Xylose-isomerases are also commonly called fructose-isomerases due to their ability to interconvert glucose and fructose. ... isomerase, D-xylose ketoisomerase, and D-xylose ketol-isomerase. The activity of D-xylose isomerase was first observed by ...
Aldose-ketose isomerases .... Apis mellifera, Ascaris suum, Bos taurus, Cavia porcellus, Homo sapiens, Klebsiella aerogenes, ... mannose-6-phosphate isomerase activity is abolished by deletion of the C-terminal 14 residues. The N-terminal region plays an ... mannose-6-phosphate isomerase activity is abolished by deletion of the C-terminal 14 residues. The N-terminal region plays an ... bifunctional enzyme with mannose-6-phosphate isomerase and sugar-1-phosphate nucleotidylyltransferase activities, EC 5.3.1.8 ...
Aldose-ketose isomerases .... Apis mellifera, Ascaris suum, Bos taurus, Cavia porcellus, Homo sapiens, Klebsiella aerogenes, ... Characterization of the Aspergillus fumigatus phosphomannose isomerase Pmi1 and its impact on cell wall synthesis and ...
The systematic name of this enzyme class is hydroxypyruvate aldose-ketose-isomerase. This enzyme participates in glyoxylate and ... This enzyme belongs to the family of isomerases, specifically those intramolecular oxidoreductases interconverting aldoses and ... de Windt FE, van der Drift C (1980). "Purification and some properties of hydroxypyruvate isomerase of Bacillus fastidiosus". ... In enzymology, a hydroxypyruvate isomerase (EC 5.3.1.22) is an enzyme that catalyzes the chemical reaction hydroxypyruvate ⇌ {\ ...
Putative tagatose-6-phosphate ketose/aldose isomerase 4.1. 0.001. SpnNT_00089. agaC_1 ...
The systematic name of this enzyme class is 11-deoxycorticosterone aldose-ketose-isomerase. Martin KO, Oh SW, Lee HJ, Monder C ... This enzyme belongs to the family of isomerases, specifically those intramolecular oxidoreductases interconverting aldoses and ... In enzymology, a corticosteroid side-chain-isomerase (EC 5.3.1.21) is an enzyme that catalyzes the chemical reaction 11- ... Monder C, Martin KO, Bogumil J (1980). "Presence of epimerase activity in hamster liver corticosteroid side chain isomerase". J ...
tagatose-6-phosphate ketose/aldose isomerase. agaV (plu0835). PTS-system, N-acetylgalactosamine-specific IIB component 2 (EIIB- ...
intramolecular isomerase activity, interconverting aldoses and ketoses. 0.016. phosphagen metabolism. 0.016. neurofilament ...
... peptidylprolyl isomerase [EC:5.2.1.8] K02082 agaS; tagatose-6-phosphate ketose/aldose isomerase [EC:5.-.-.-] K02558 mpl; UDP-N- ... VV2_1023 Putative tagatose-6-phosphate ketose/aldose isomerase VV1_0706 mpl; UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- ... VV2_0526 FKBP-type peptidyl-prolyl cis-trans isomerase / Macrophage infectivity potentiator ...
Aldose-Ketose Isomerases. MESH. Amino Acid Sequence. MESH. Binding Sites. MESH. Carbohydrate Epimerases/chemistry. MESH. ... Crystal structure at 2.0 A resolution of phosphoribosyl anthranilate isomerase from the hyperthermophile Thermotoga maritima: ... To this end the crystal structure of dimeric phosphoribosyl anthranilate isomerase from the hyperthermophile Thermotoga ... To this end the crystal structure of dimeric phosphoribosyl anthranilate isomerase from the hyperthermophile Thermotoga ...
Aldose-Ketose Isomerases. MESH. Amino Acid Sequence. MESH. Bacterial Proteins/genetics. MESH. ... phosphoribosyl anthranilate isomerase) and trpA (alpha-subunit of tryptophan synthase) suggest that these thermostable (beta ...
SIS domains (2) are commonly found in aldose-ketose isomerases and are believed to be the portion of the protein responsible ... coli A5P isomerases. When aligned individually with the SIS domains of the other three A5P isomerases, the c3406 protein shares ... isomerases (see below). A5P isomerases catalyze the interconversion of d-ribulose 5-phosphate (Ru5P), a product of the pentose ... and encodes an A5P isomerase (15) that is involved in the metabolism of sorbitol. A third A5P isomerase is found in E. coli ...
Alcohol Dehydrogenase/genetics, Aldehyde Oxidoreductases/genetics, Aldose-Ketose Isomerases/genetics, Animals, Bacterial ... glucosamine-6-phosphate isomerase, and alcohol dehydrogenase E. These four genes have a limited distribution among sequenced ...
Collyer CA, Henrick K, Blow DM (1990) Mechanism for aldose-ketose interconversion by D-xylose isomerase involving ring opening ... Carrell HL, Glusker JP, Burger V, Manfre F, Tritsch D, Biellmann JF (1989) X-ray analysis of D-xylose isomerase at 1.9 A: ... Carrell HL, Hoier H, Glusker JP (1994) Modes of binding substrates and their analogues to the enzyme D-xylose isomerase. Acta ... Yoshida H, Yoshihara A, Teraoka M, Yamashita S, Izumori K, Kamitori S (2012) Structure of L-rhamnose isomerase in complex with ...
GO:0016861 intramolecular oxidoreductase activity, interconverting aldoses and ketoses Cellular Component. No terms assigned in ... KduI/IolB isomerase (IPR021120). Short name: KduI/IolB_isomerase Family relationships *KduI/IolB isomerase (IPR021120) *5-deoxy ... The KduI/IolB family of enzymes includes 5-keto 4-deoxyuronate isomerase (KduI) and 5-deoxy-glucuronate isomerase (IolB). ... The crystal structure of 5-keto-4-deoxyuronate isomerase from Escherichia coli.. Proteins 61 680-4 2005 ...
The food enzyme is a d‐xylose aldoseketoseisomerase (EC 5.3.1.5) produced with the genetically modified Streptomyces ... Safety evaluation of the food enzyme xylose isomerase from the genetically modified Streptomyces rubiginosus strain DP‐Pzn37. ...
They include enzymes converting aldoses to ketoses (ALDOSE-KETOSE ISOMERASES), enzymes shifting a carbon-carbon double bond ( ... CARBON-CARBON DOUBLE BOND ISOMERASES), and enzymes transposing S-S bonds (SULFUR-SULFUR BOND ISOMERASES). (From Enzyme ... Enzymes of the isomerase class that catalyze the oxidation of one part of a molecule with a corresponding reduction of another ... Carbon-carbon Double Bond Isomerases. Enzymes that catalyze the shifting of a carbon-carbon double bond from one position to ...
Purchase high purity enzyme Phosphoglucose isomerase (S. cerevisiae) for use in research, biochemical enzyme assays and in ... glucose-6-phosphate isomerise; D-glucose-6-phosphate aldose-ketose-isomerase. Recombinant. From Saccharomyces cerevisiae.. In ... High purity Phosphoglucose isomerase (Saccharomyces cerevisiae) for use in research, biochemical enzyme assays and in vitro ... One unit of phosphoglucose isomerase activity is the amount of enzyme required to convert one µmole of D-fructose 6-phosphate ...
Aldose-Ketose Isomerases. 3. + +. 76. Isocitrate Lyase. 3. + +. 77. Quinine. 3. + +. 78. Polymyxin B. 3. + +. ...
... measurements of phosphoglucose isomerase and phosphomannose isomerase by direct analysis of phosphorylated aldose-ketose ... Wierenga RK, Kapetaniou EG, Venkatesan R. Triosephosphate isomerase: a highly evolved biocatalyst. Cell Mol Life Sci. 2010;67( ... First, among the 17 enzyme-constrained reactions, we identified triose-phosphate isomerase (TPI), glyceraldehyde-3-phosphate ... for which the glucose-6-phosphate isomerase (PGI), MDH, glucose 6-phosphate dehydrogenase (G6PDH), succinate dehydrogenase ( ...
... measurements of phosphoglucose isomerase and phosphomannose isomerase by direct analysis of phosphorylated aldose-ketose ... Induction of aldose reductase and xylitol dehydrogenase activities in Candida tenuis CBS 4435. FEMS Microbiol Lett. 1997;149(1 ... Development of efficient xylose fermentation in Saccharomyces cerevisiae: xylose isomerase as a key component. Adv Biochem Eng ... Time-dependent utilization of NADPH (XR) or NADH [XR, alcohol dehydrogenase (ADH), XK, phosphoglucose isomerase (PGI), 6- ...
... that were shown to provide xylose isomerase activity in yeast cells. The xylose isomerase activity can complete a xylose ... C12Y503/01-Intramolecular oxidoreductases (5.3) interconverting aldoses and ketoses (5.3.1) * C12Y503/01005-Xylose isomerase ( ... Xylose Isomerase. Expression of xylose isomerases in yeast cells has been problematic; in particular, many bacterial xylose ... US8906656B2 - Cow rumen xylose isomerases active in yeast cells - Google Patents. Cow rumen xylose isomerases active in yeast ...
  • Analysis of this gene cluster reveals that one of the genes, c3406, encodes a protein with significant homology to the sugar isomerase (SIS) domain of arabinose 5-phosphate (A5P) isomerases (see below). (asm.org)
  • The sugar isomerase (SIS) domain is a phosphosugar-binding module that is found in a variety of eubacterial, archaebacterial and eukaryotic proteins that have a role in phosphosugar isomerization or regulation [ 1 ]. (expasy.org)
  • Here we reported that a Drosophila strain with reduced expression of ribose-5-phosphate isomerase (rpi), EP2456, exhibits increased resistance to oxidative stress and enhanced lifespan. (isharonline.org)
  • We prepared recombinant c3406 protein, found it to possess arabinose 5-phosphate isomerase activity, and characterized this activity in detail. (asm.org)
  • Thus, CtAPI represents the first d -arabinose-5-phosphate isomerase to be identified and characterized from a Gram-positive bacterium. (asm.org)
  • The tertiary structure was determined for several xylose isomerases from microbes starting in the mid 1980s (Streptomyces olivochromogenes in 1988, Streptomyces violaceoniger in 1988, Streptomyces rubiginosus in 1984, Arthrobacter B3728 in 1986, Actinoplanes missouriensis in 1992, and Clostridium thermosulfurogenes in 1990). (wikipedia.org)
  • A5P isomerases catalyze the interconversion of d -ribulose 5-phosphate (Ru5P), a product of the pentose phosphate pathway, and A5P, an important intermediate in LPS and capsular polysaccharide biosynthesis ( 25 ). (asm.org)
  • d -Arabinose-5-phosphate (A5P) isomerases (APIs) catalyze the interconversion of d -ribulose-5-phosphate and d -arabinose-5-phosphate. (asm.org)
  • In Gram-negative microorganisms, d -arabinose-5-phosphate (A5P) isomerase (API) catalyzes the interconversion of d -ribulose-5-phosphate (Ru5P), a product of the pentose phosphate pathway, and d -arabinose-5-phosphate. (asm.org)
  • In the present study novel tetrose isomerizations and C-2 epimerizations with both the D- and L-forms of the sugars by an industrial xylose isomerase (XI) from Streptomyces rubiginosus were described. (aalto.fi)
  • Here we present gene distribution and phylogenetic analyses of the genes encoding the hybrid-cluster protein, A-type flavoprotein, glucosamine-6-phosphate isomerase, and alcohol dehydrogenase E. These four genes have a limited distribution among sequenced prokaryotic and eukaryotic genomes and were previously implicated in gene transfer events affecting eukaryotes. (diva-portal.org)
  • As a matter of convenience, however, the different isomerases have been ordered in this chapter according to the number of carbon atoms in the carbohydrates on which they act. (springer.com)
  • Thermal tests were also done by Kei Y. and Noritaka T. and the xylose isomerase was found to be thermally stable to about 60 degrees Celsius Xylose isomerase has a structure that is based on eight alpha/beta barrels that create an active site holding two divalent magnesium ions. (wikipedia.org)
  • Fructose 1,6, phosphate is then formed by the phosphorylation of ketose-6-phosphate, which is the committed step, and this molecule is cleaved into a three carbon compound glyceraldehyde-3-phosphate. (oxbridgenotes.co.uk)