An enzyme that oxidizes an aldehyde in the presence of NAD+ and water to an acid and NADH. This enzyme was formerly classified as EC 1.1.1.70.
Organic compounds containing a carbonyl group in the form -CHO.
Oxidoreductases that are specific for ALDEHYDES.
A carbamate derivative used as an alcohol deterrent. It is a relatively nontoxic substance when administered alone, but markedly alters the intermediary metabolism of alcohol. When alcohol is ingested after administration of disulfiram, blood acetaldehyde concentrations are increased, followed by flushing, systemic vasodilation, respiratory difficulties, nausea, hypotension, and other symptoms (acetaldehyde syndrome). It acts by inhibiting aldehyde dehydrogenase.
A colorless, flammable liquid used in the manufacture of acetic acid, perfumes, and flavors. It is also an intermediate in the metabolism of alcohol. It has a general narcotic action and also causes irritation of mucous membranes. Large doses may cause death from respiratory paralysis.
A metalloflavoprotein enzyme involved the metabolism of VITAMIN A, this enzyme catalyzes the oxidation of RETINAL to RETINOIC ACID, using both NAD+ and FAD coenzymes. It also acts on both the 11-trans- and 13-cis-forms of RETINAL.
A zinc-containing enzyme which oxidizes primary and secondary alcohols or hemiacetals in the presence of NAD. In alcoholic fermentation, it catalyzes the final step of reducing an aldehyde to an alcohol in the presence of NADH and hydrogen.
An NAD+ dependent enzyme that catalyzes the oxidation of betain aldehyde to BETAINE.
A cyanide compound which has been used as a fertilizer, defoliant and in many manufacturing processes. It often occurs as the calcium salt, sometimes also referred to as cyanamide. The citrated calcium salt is used in the treatment of alcoholism.
A coenzyme composed of ribosylnicotinamide 5'-diphosphate coupled to adenosine 5'-phosphate by pyrophosphate linkage. It is found widely in nature and is involved in numerous enzymatic reactions in which it serves as an electron carrier by being alternately oxidized (NAD+) and reduced (NADH). (Dorland, 27th ed)
A tetrameric enzyme that, along with the coenzyme NAD+, catalyzes the interconversion of LACTATE and PYRUVATE. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.
A subclass of enzymes which includes all dehydrogenases acting on primary and secondary alcohols as well as hemiacetals. They are further classified according to the acceptor which can be NAD+ or NADP+ (subclass 1.1.1), cytochrome (1.1.2), oxygen (1.1.3), quinone (1.1.5), or another acceptor (1.1.99).
Enzymes that catalyze the dehydrogenation of GLYCERALDEHYDE 3-PHOSPHATE. Several types of glyceraldehyde-3-phosphate-dehydrogenase exist including phosphorylating and non-phosphorylating varieties and ones that transfer hydrogen to NADP and ones that transfer hydrogen to NAD.
Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.
An enzyme that catalyzes the conversion of L-glutamate and water to 2-oxoglutarate and NH3 in the presence of NAD+. (From Enzyme Nomenclature, 1992) EC 1.4.1.2.
An autosomal recessive neurocutaneous disorder characterized by severe ichthyosis MENTAL RETARDATION; SPASTIC PARAPLEGIA; and congenital ICHTHYOSIS. It is caused by mutation of gene encoding microsomal fatty ALDEHYDE DEHYDROGENASE leading to defect in fatty alcohol metabolism.
An enzyme that catalyzes the conversion of (S)-malate and NAD+ to oxaloacetate and NADH. EC 1.1.1.37.
An enzyme of the oxidoreductase class that catalyzes the conversion of isocitrate and NAD+ to yield 2-ketoglutarate, carbon dioxide, and NADH. It occurs in cell mitochondria. The enzyme requires Mg2+, Mn2+; it is activated by ADP, citrate, and Ca2+, and inhibited by NADH, NADPH, and ATP. The reaction is the key rate-limiting step of the citric acid (tricarboxylic) cycle. (From Dorland, 27th ed) (The NADP+ enzyme is EC 1.1.1.42.) EC 1.1.1.41.
A group of nitrogen mustard compounds which are substituted with a phosphoramide group or its derivatives. They are usually cytotoxic and used as antineoplastic agents.
The rate dynamics in chemical or physical systems.
Nicotinamide adenine dinucleotide phosphate. A coenzyme composed of ribosylnicotinamide 5'-phosphate (NMN) coupled by pyrophosphate linkage to the 5'-phosphate adenosine 2',5'-bisphosphate. It serves as an electron carrier in a number of reactions, being alternately oxidized (NADP+) and reduced (NADPH). (Dorland, 27th ed)
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
A hypnotic and sedative used in the treatment of INSOMNIA.
An alcohol oxidoreductase which catalyzes the oxidation of L-iditol to L-sorbose in the presence of NAD. It also acts on D-glucitol to form D-fructose. It also acts on other closely related sugar alcohols to form the corresponding sugar. EC 1.1.1.14
A flavoprotein containing oxidoreductase that catalyzes the reduction of lipoamide by NADH to yield dihydrolipoamide and NAD+. The enzyme is a component of several MULTIENZYME COMPLEXES.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Reversibly catalyze the oxidation of a hydroxyl group of carbohydrates to form a keto sugar, aldehyde or lactone. Any acceptor except molecular oxygen is permitted. Includes EC 1.1.1.; EC 1.1.2.; and 1.1.99.
A flavoprotein containing oxidoreductase that catalyzes the dehydrogenation of SUCCINATE to fumarate. In most eukaryotic organisms this enzyme is a component of mitochondrial electron transport complex II.
Mitochondria in hepatocytes. As in all mitochondria, there are an outer membrane and an inner membrane, together creating two separate mitochondrial compartments: the internal matrix space and a much narrower intermembrane space. In the liver mitochondrion, an estimated 67% of the total mitochondrial proteins is located in the matrix. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p343-4)
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
A clear, colorless liquid rapidly absorbed from the gastrointestinal tract and distributed throughout the body. It has bactericidal activity and is used often as a topical disinfectant. It is widely used as a solvent and preservative in pharmaceutical preparations as well as serving as the primary ingredient in ALCOHOLIC BEVERAGES.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).
An enzyme that catalyzes reversibly the oxidation of an aldose to an alditol. It possesses broad specificity for many aldoses. EC 1.1.1.21.
An enzyme that plays a role in the VALINE; LEUCINE; and ISOLEUCINE catabolic pathways by catalyzing the oxidation of 2-methyl-3-oxopropanate to propanoyl-CoA using NAD+ as a coenzyme. Methylmalonate semialdehyde dehydrogenase deficiency is characterized by elevated BETA-ALANINE and 3-hydropropionic acid.
An enzyme that plays a role in the GLUTAMATE and butanoate metabolism pathways by catalyzing the oxidation of succinate semialdehyde to SUCCINATE using NAD+ as a coenzyme. Deficiency of this enzyme, causes 4-hydroxybutyricaciduria, a rare inborn error in the metabolism of the neurotransmitter 4-aminobutyric acid (GABA).
A transient reddening of the face that may be due to fever, certain drugs, exertion, stress, or a disease process.
Enzymes of the oxidoreductase class that catalyze the dehydrogenation of hydroxysteroids. (From Enzyme Nomenclature, 1992) EC 1.1.-.
A glucose dehydrogenase that catalyzes the oxidation of beta-D-glucose to form D-glucono-1,5-lactone, using NAD as well as NADP as a coenzyme.
Reversibly catalyzes the oxidation of a hydroxyl group of sugar alcohols to form a keto sugar, aldehyde or lactone. Any acceptor except molecular oxygen is permitted. Includes EC 1.1.1.; EC 1.1.2. and EC 1.1.99.
Small molecules that are required for the catalytic function of ENZYMES. Many VITAMINS are coenzymes.
Catalyze the oxidation of 3-hydroxysteroids to 3-ketosteroids.
Used in the form of its salts as a dye and as an intermediate in manufacture of Acid Yellow, diazo dyes, and indulines.
An enzyme that catalyzes the oxidation of XANTHINE in the presence of NAD+ to form URIC ACID and NADH. It acts also on a variety of other purines and aldehydes.
D-Glucose:1-oxidoreductases. Catalyzes the oxidation of D-glucose to D-glucono-gamma-lactone and reduced acceptor. Any acceptor except molecular oxygen is permitted. Includes EC 1.1.1.47; EC 1.1.1.118; EC 1.1.1.119 and EC 1.1.99.10.
An enzyme of the oxidoreductase class that catalyzes the reaction 6-phospho-D-gluconate and NADP+ to yield D-ribulose 5-phosphate, carbon dioxide, and NADPH. The reaction is a step in the pentose phosphate pathway of glucose metabolism. (From Dorland, 27th ed) EC 1.1.1.43.
Enzymes that catalyze the first step in the beta-oxidation of FATTY ACIDS.
The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)
A flavoprotein and iron sulfur-containing oxidoreductase that catalyzes the oxidation of NADH to NAD. In eukaryotes the enzyme can be found as a component of mitochondrial electron transport complex I. Under experimental conditions the enzyme can use CYTOCHROME C GROUP as the reducing cofactor. The enzyme was formerly listed as EC 1.6.2.1.
An enzyme that catalyzes the dehydrogenation of inosine 5'-phosphate to xanthosine 5'-phosphate in the presence of NAD. EC 1.1.1.205.
Alcohol oxidoreductases with substrate specificity for LACTIC ACID.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Acyclic branched or unbranched hydrocarbons having two carbon-carbon double bonds.
Flavoproteins that catalyze reversibly the reduction of carbon dioxide to formate. Many compounds can act as acceptors, but the only physiologically active acceptor is NAD. The enzymes are active in the fermentation of sugars and other compounds to carbon dioxide and are the key enzymes in obtaining energy when bacteria are grown on formate as the main carbon source. They have been purified from bovine blood. EC 1.2.1.2.
A reagent used for the determination of iron.
A flavoprotein oxidoreductase that has specificity for medium-chain fatty acids. It forms a complex with ELECTRON TRANSFERRING FLAVOPROTEINS and conveys reducing equivalents to UBIQUINONE.
A class of enzymes that catalyzes the oxidation of 17-hydroxysteroids to 17-ketosteroids. EC 1.1.-.
Highly proliferative, self-renewing, and colony-forming stem cells which give rise to NEOPLASMS.
Alkyl compounds containing a hydroxyl group. They are classified according to relation of the carbon atom: primary alcohols, R-CH2OH; secondary alcohols, R2-CHOH; tertiary alcohols, R3-COH. (From Grant & Hackh's Chemical Dictionary, 5th ed)
The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
A ketone oxidoreductase that catalyzes the overall conversion of alpha-keto acids to ACYL-CoA and CO2. The enzyme requires THIAMINE DIPHOSPHATE as a cofactor. Defects in genes that code for subunits of the enzyme are a cause of MAPLE SYRUP URINE DISEASE. The enzyme was formerly classified as EC 1.2.4.3.
Enzymes catalyzing the dehydrogenation of secondary amines, introducing a C=N double bond as the primary reaction. In some cases this is later hydrolyzed.
A volatile vasodilator which relieves ANGINA PECTORIS by stimulating GUANYLATE CYCLASE and lowering cytosolic calcium. It is also sometimes used for TOCOLYSIS and explosives.
A chelating agent that has been used to mobilize toxic metals from the tissues of humans and experimental animals. It is the main metabolite of DISULFIRAM.
Oxidoreductases that are specific for KETONES.
The E1 component of the multienzyme PYRUVATE DEHYDROGENASE COMPLEX. It is composed of 2 alpha subunits (pyruvate dehydrogenase E1 alpha subunit) and 2 beta subunits (pyruvate dehydrogenase E1 beta subunit).
Enzymes that reversibly catalyze the oxidation of a 3-hydroxyacyl CoA to 3-ketoacyl CoA in the presence of NAD. They are key enzymes in the oxidation of fatty acids and in mitochondrial fatty acid synthesis.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Hydroxysteroid dehydrogenases that catalyzes the reversible conversion of CORTISOL to the inactive metabolite CORTISONE. Enzymes in this class can utilize either NAD or NADP as cofactors.
A vasodilator with general properties similar to NITROGLYCERIN but with a more prolonged duration of action. (From Martindale, The Extra Pharmacopoeia, 30th ed, p1025)
Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components.
An enzyme that catalyzes the oxidation of UDPglucose to UDPglucuronate in the presence of NAD+. EC 1.1.1.22.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
The sum of the weight of all the atoms in a molecule.
A carotenoid constituent of visual pigments. It is the oxidized form of retinol which functions as the active component of the visual cycle. It is bound to the protein opsin forming the complex rhodopsin. When stimulated by visible light, the retinal component of the rhodopsin complex undergoes isomerization at the 11-position of the double bond to the cis-form; this is reversed in "dark" reactions to return to the native trans-configuration.
An oxidoreductase involved in pyrimidine base degradation. It catalyzes the catabolism of THYMINE; URACIL and the chemotherapeutic drug, 5-FLUOROURACIL.
Semiautonomous, self-reproducing organelles that occur in the cytoplasm of all cells of most, but not all, eukaryotes. Each mitochondrion is surrounded by a double limiting membrane. The inner membrane is highly invaginated, and its projections are called cristae. Mitochondria are the sites of the reactions of oxidative phosphorylation, which result in the formation of ATP. They contain distinctive RIBOSOMES, transfer RNAs (RNA, TRANSFER); AMINO ACYL T RNA SYNTHETASES; and elongation and termination factors. Mitochondria depend upon genes within the nucleus of the cells in which they reside for many essential messenger RNAs (RNA, MESSENGER). Mitochondria are believed to have arisen from aerobic bacteria that established a symbiotic relationship with primitive protoeukaryotes. (King & Stansfield, A Dictionary of Genetics, 4th ed)
A naturally occurring compound that has been of interest for its role in osmoregulation. As a drug, betaine hydrochloride has been used as a source of hydrochloric acid in the treatment of hypochlorhydria. Betaine has also been used in the treatment of liver disorders, for hyperkalemia, for homocystinuria, and for gastrointestinal disturbances. (From Martindale, The Extra Pharmacopoeia, 30th ed, p1341)
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in enzyme synthesis.
Substances interfering with the metabolism of ethyl alcohol, causing unpleasant side effects thought to discourage the drinking of alcoholic beverages. Alcohol deterrents are used in the treatment of alcoholism.
A 3-hydroxysteroid dehydrogenase which catalyzes the reversible reduction of the active androgen, DIHYDROTESTOSTERONE to 5 ALPHA-ANDROSTANE-3 ALPHA,17 BETA-DIOL. It also has activity towards other 3-alpha-hydroxysteroids and on 9-, 11- and 15- hydroxyprostaglandins. The enzyme is B-specific in reference to the orientation of reduced NAD or NADPH.
A disease-producing enzyme deficiency subject to many variants, some of which cause a deficiency of GLUCOSE-6-PHOSPHATE DEHYDROGENASE activity in erythrocytes, leading to hemolytic anemia.
An increase in the rate of synthesis of an enzyme due to the presence of an inducer which acts to derepress the gene responsible for enzyme synthesis.
A low-affinity 11 beta-hydroxysteroid dehydrogenase found in a variety of tissues, most notably in LIVER; LUNG; ADIPOSE TISSUE; vascular tissue; OVARY; and the CENTRAL NERVOUS SYSTEM. The enzyme acts reversibly and can use either NAD or NADP as cofactors.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
The phenomenon whereby compounds whose molecules have the same number and kind of atoms and the same atomic arrangement, but differ in their spatial relationships. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)
An NAD-dependent enzyme that catalyzes the reversible DEAMINATION of L-ALANINE to PYRUVATE and AMMONIA. The enzyme is needed for growth when ALANINE is the sole CARBON or NITROGEN source. It may also play a role in CELL WALL synthesis because L-ALANINE is an important constituent of the PEPTIDOGLYCAN layer.
Any of the ruminant mammals with curved horns in the genus Ovis, family Bovidae. They possess lachrymal grooves and interdigital glands, which are absent in GOATS.
Catalyzes reversibly the oxidation of hydroxyl groups of prostaglandins.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
Sugar alcohol dehydrogenases that have specificity for MANNITOL. Enzymes in this category are generally classified according to their preference for a specific reducing cofactor.
Usually high-molecular-weight, straight-chain primary alcohols, but can also range from as few as 4 carbons, derived from natural fats and oils, including lauryl, stearyl, oleyl, and linoleyl alcohols. They are used in pharmaceuticals, cosmetics, detergents, plastics, and lube oils and in textile manufacture. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)
Electrophoresis in which a pH gradient is established in a gel medium and proteins migrate until they reach the site (or focus) at which the pH is equal to their isoelectric point.
A flavoprotein oxidoreductase that has specificity for short-chain fatty acids. It forms a complex with ELECTRON-TRANSFERRING FLAVOPROTEINS and conveys reducing equivalents to UBIQUINONE.
The art or process of comparing photometrically the relative intensities of the light in different parts of the spectrum.
A genus of gram-negative, aerobic, rod-shaped bacteria widely distributed in nature. Some species are pathogenic for humans, animals, and plants.
A group of enzymes that catalyze the reversible reduction-oxidation reaction of 20-hydroxysteroids, such as from a 20-ketosteroid to a 20-alpha-hydroxysteroid (EC 1.1.1.149) or to a 20-beta-hydroxysteroid (EC 1.1.1.53).
Proteins prepared by recombinant DNA technology.
An high-affinity, NAD-dependent 11-beta-hydroxysteroid dehydrogenase that acts unidirectionally to catalyze the dehydrogenation of CORTISOL to CORTISONE. It is found predominantly in mineralocorticoid target tissues such as the KIDNEY; COLON; SWEAT GLANDS; and the PLACENTA. Absence of the enzyme leads to a fatal form of childhood hypertension termed, APPARENT MINERALOCORTICOID EXCESS SYNDROME.
A sulfonylurea hypoglycemic agent used in the treatment of non-insulin-dependent diabetes mellitus not responding to dietary modification. (From Martindale, The Extra Pharmacopoeia, 30th ed, p277)
Proteins encoded by the mitochondrial genome or proteins encoded by the nuclear genome that are imported to and resident in the MITOCHONDRIA.
A flavoprotein oxidoreductase that has specificity for long-chain fatty acids. It forms a complex with ELECTRON-TRANSFERRING FLAVOPROTEINS and conveys reducing equivalents to UBIQUINONE.
Compounds based on benzene fused to oxole. They can be formed from methylated CATECHOLS such as EUGENOL.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
An enzyme that catalyzes the reduction of aspartic beta-semialdehyde to homoserine, which is the branch point in biosynthesis of methionine, lysine, threonine and leucine from aspartic acid. EC 1.1.1.3.
An important regulator of GENE EXPRESSION during growth and development, and in NEOPLASMS. Tretinoin, also known as retinoic acid and derived from maternal VITAMIN A, is essential for normal GROWTH; and EMBRYONIC DEVELOPMENT. An excess of tretinoin can be teratogenic. It is used in the treatment of PSORIASIS; ACNE VULGARIS; and several other SKIN DISEASES. It has also been approved for use in promyelocytic leukemia (LEUKEMIA, PROMYELOCYTIC, ACUTE).
The functional hereditary units of BACTERIA.
Reduction of pharmacologic activity or toxicity of a drug or other foreign substance by a living system, usually by enzymatic action. It includes those metabolic transformations that make the substance more soluble for faster renal excretion.
An enzyme bound to the inner mitochondrial membrane that catalyzes the oxidation of CHOLINE to BETAINE.
A mitochondrial flavoprotein, this enzyme catalyzes the oxidation of 3-methylbutanoyl-CoA to 3-methylbut-2-enoyl-CoA using FAD as a cofactor. Defects in the enzyme, is associated with isovaleric acidemia (IVA).
An NAD+ dependent enzyme that catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate to 3-carboxy-4-methyl-2-oxopentanoate. It is involved in the biosynthesis of VALINE; LEUCINE; and ISOLEUCINE.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Bifunctional cross-linking agent that links covalently free amino groups of proteins or polypeptides, including those in cell membranes. It is used as reagent or fixative in immunohistochemistry and is a proposed antisickling agent.
The chemical alteration of an exogenous substance by or in a biological system. The alteration may inactivate the compound or it may result in the production of an active metabolite of an inactive parent compound. The alterations may be divided into METABOLIC DETOXICATION, PHASE I and METABOLIC DETOXICATION, PHASE II.
Large, hoofed mammals of the family EQUIDAE. Horses are active day and night with most of the day spent seeking and consuming food. Feeding peaks occur in the early morning and late afternoon, and there are several daily periods of rest.
(Pyruvate dehydrogenase (lipoamide))-phosphate phosphohydrolase. A mitochondrial enzyme that catalyzes the hydrolytic removal of a phosphate on a specific seryl hydroxyl group of pyruvate dehydrogenase, reactivating the enzyme complex. EC 3.1.3.43.
An octameric enzyme belonging to the superfamily of amino acid dehydrogenases. Leucine dehydrogenase catalyzes the reversible oxidative deamination of L-LEUCINE, to 4-methyl-2-oxopentanoate (2-ketoisocaproate) and AMMONIA, with the corresponding reduction of the cofactor NAD+.
Systems of enzymes which function sequentially by catalyzing consecutive reactions linked by common metabolic intermediates. They may involve simply a transfer of water molecules or hydrogen atoms and may be associated with large supramolecular structures such as MITOCHONDRIA or RIBOSOMES.
An enzyme that catalyzes the oxidation of 3-phosphoglycerate to 3-phosphohydroxypyruvate. It takes part in the L-SERINE biosynthesis pathway.
The extent to which an enzyme retains its structural conformation or its activity when subjected to storage, isolation, and purification or various other physical or chemical manipulations, including proteolytic enzymes and heat.
The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.
A basic science concerned with the composition, structure, and properties of matter; and the reactions that occur between substances and the associated energy exchange.
Enzymes that catalyze the oxidation of estradiol at the 17-hydroxyl group in the presence of NAD+ or NADP+ to yield estrone and NADH or NADPH. The 17-hydroxyl group can be in the alpha- or beta-configuration. EC 1.1.1.62
Behaviors associated with the ingesting of alcoholic beverages, including social drinking.
The composition, conformation, and properties of atoms and molecules, and their reaction and interaction processes.
The generic name for the group of aliphatic hydrocarbons Cn-H2n+2. They are denoted by the suffix -ane. (Grant & Hackh's Chemical Dictionary, 5th ed)
Derivatives of ACETIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain the carboxymethane structure.
The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990)
A genus of VIBRIONACEAE, made up of short, slightly curved, motile, gram-negative rods. Various species produce cholera and other gastrointestinal disorders as well as abortion in sheep and cattle.
Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.

Inhibitory sites in enzymes: zinc removal and reactivation by thionein. (1/1111)

Thionein (T) has not been isolated previously from biological material. However, it is generated transiently in situ by removal of zinc from metallothionein under oxidoreductive conditions, particularly in the presence of selenium compounds. T very rapidly activates a group of enzymes in which zinc is bound at an inhibitory site. The reaction is selective, as is apparent from the fact that T does not remove zinc from the catalytic sites of zinc metalloenzymes. T instantaneously reverses the zinc inhibition with a stoichiometry commensurate with its known capacity to bind seven zinc atoms in the form of clusters in metallothionein. The zinc inhibition is much more pronounced than was previously reported, with dissociation constants in the low nanomolar range. Thus, T is an effective, endogenous chelating agent, suggesting the existence of a hitherto unknown and unrecognized biological regulatory system. T removes the metal from an inhibitory zinc-specific enzymatic site with a resultant marked increase of activity. The potential significance of this system is supported by the demonstration of its operations in enzymes involved in glycolysis and signal transduction.  (+info)

Xenopus cytosolic thyroid hormone-binding protein (xCTBP) is aldehyde dehydrogenase catalyzing the formation of retinoic acid. (2/1111)

Amino acid sequencing of an internal peptide fragment derived from purified Xenopus cytosolic thyroid hormone-binding protein (xCTBP) demonstrates high similarity to the corresponding sequence of mammalian aldehyde dehydrogenase 1 (ALDH1) (Yamauchi, K., and Tata, J. R. (1994) Eur. J. Biochem. 225, 1105-1112). Here we show that xCTBP was co-purified with ALDH and 3,3',5-triiodo-L-thyronine (T3) binding activities. By photoaffinity labeling with [125I]T3, a T3-binding site in the xCTBP was estimated to reside in amino acid residues 93-114, which is distinct from the active site of the enzyme but present in the NAD+ binding domain. The amino acid sequences deduced from the two isolated xALDH1 cDNAs (xALDH1-I and xALDH1-II) were 94.6% identical to each other and very similar to those of mammalian ALDH1 enzymes. The two recombinant xALDH1 proteins exhibit both T3 binding activity and ALDH activity converting retinal to retinoic acid (RA), which are similar to those of xCTBP. The mRNAs were present abundantly in kidney and intestine of adult female Xenopus. Interestingly, their T3 binding activities were inhibited by NAD+ and NADH but not by NADP+ and NADPH, whereas NAD+ was required for their ALDH activities. Our results demonstrate that xCTBP is identical to ALDH1 and suggest that this protein might modulate RA synthesis and intracellular level of free T3.  (+info)

Stimulation of premature retinoic acid synthesis in Xenopus embryos following premature expression of aldehyde dehydrogenase ALDH1. (3/1111)

In order for nuclear retinoic acid receptors to mediate retinoid signaling, the ligand retinoic acid must first be produced from its vitamin A precursor retinal. Biochemical studies have shown that retinal can be metabolized in vitro to retinoic acid by members of the aldehyde dehydrogenase enzyme family, including ALDH1. Here we describe the first direct evidence that ALDH1 plays a physiological role in retinoic acid synthesis by analysis of retinoid signaling in Xenopus embryos, which have plentiful stores of maternally derived retinal. The Xenopus ALDH1 gene was cloned and shown to be highly conserved with chick and mammalian homologs. Xenopus ALDH1 was not expressed at blastula and gastrula stages, but was expressed at the neurula stage. We used a retinoic acid bioassay to demonstrate that retinoic acid is normally undetectable in embryos from fertilization to the initial gastrula stage, but that a tremendous increase in retinoic acid occurs during neurulation when ALDH1 is first expressed. Overexpression of ALDH1 by injection of Xenopus embryos with mRNAs encoding the mouse, chick or Xenopus ALDH1 homologs induced high levels of retinoic acid detection during the blastula stage. Thus, premature expression of ALDH1 stimulates premature synthesis of retinoic acid. These findings reveal an important conserved role for ALDH1 in retinoic acid synthesis in vivo, and demonstrate that conversion of retinoids from the aldehyde form to the carboxylic acid form is a crucial regulatory step in retinoid signaling.  (+info)

The negative regulation of the rat aldehyde dehydrogenase 3 gene by glucocorticoids: involvement of a single imperfect palindromic glucocorticoid responsive element. (4/1111)

Glucocorticoids repressed the polycyclic aromatic hydrocarbon-dependent induction of Class 3 aldehyde dehydrogenase (ALDH3) enzyme activity and mRNA levels in isolated rat hepatocytes by more than 50 to 80%, with a concentration-dependence consistent with the involvement of the glucocorticoid receptor (GR). No consistent effect on the low basal transcription rate was observed. This effect of glucocorticoids (GC) on polycyclic aromatic hydrocarbon induction was effectively antagonized at the mRNA and protein level by the GR antagonist RU38486. The response was cycloheximide-sensitive, because the protein synthesis inhibitor caused a GC-dependent superinduction of ALDH3 mRNA levels. This suggests that the effects of GC on this gene are complex and both positive and negative gene regulation is possible. The GC-response was recapitulated in HepG2 cells using transient transfection experiments with CAT reporter constructs containing 3.5 kb of 5'-flanking region from ALDH3. This ligand-dependent response was also observed when a chimeric GR (GR DNA-binding domain and peroxisome proliferator-activated receptor ligand-binding domain) was used in place of GR in the presence of the peroxisome proliferator, nafenopin. A putative palindromic glucocorticoid-responsive element exists between -930 and -910 base pairs relative to the transcription start site. If this element was either deleted or mutated, the negative GC-response was completely lost, which suggests that this sequence is responsible, in part, for the negative regulation of the gene. Electrophoretic mobility shift analysis demonstrated that this palindromic glucocorticoid-responsive element is capable of forming a specific DNA-protein complex with human glucocorticoid receptor. In conclusion, the negative regulation of ALDH3 in rat liver is probably mediated through direct GR binding to its canonical responsive element.  (+info)

Molecular analysis of two closely related mouse aldehyde dehydrogenase genes: identification of a role for Aldh1, but not Aldh-pb, in the biosynthesis of retinoic acid. (5/1111)

Mammalian class I aldehyde dehydrogenase (ALDH1) has been implicated as a retinal dehydrogenase in the biosynthesis of retinoic acid, a modulator of gene expression and cell differentiation. As the first step towards studying the regulation of ALDH1 and its physiological role in the biosynthesis of retinoic acid, mouse ALDH1 cDNA and genomic clones have been characterized. During the cloning process, an additional closely related gene was also isolated and named Aldh-pb, owing to its high amino acid sequence identity (92%) with the rat phenobarbitol-inducible ALDH protein (ALDH-PB). Aldh1 spans about 45 kb in length, whereas Aldh-pb spans about 35 kb. Both genes are composed of 13 exons, and the positions of all the exon/intron boundaries are conserved with those of human ALDH1. The promoter regions of Aldh1 and Aldh-pb demonstrate high sequence similarity with those of human ALDH1 and rat ALDH-PB. Expression of Aldh1 and Aldh-pb is tissue-specific, with mRNAs for both genes being found in the liver, lung and testis, but not in the heart, spleen or muscle. Expression of Aldh-pb, but not Aldh1, was also detected at high levels in the kidney. Aldh1 and Aldh-pb encode proteins of 501 amino acids with 90% positional identity. To examine the relative roles of these two enzymes in retinoic acid synthesis in vivo, Xenopus embryos were injected with mRNAs encoding these enzymes to assay the effect on conversion of endogenous retinal into retinoic acid. Injection of ALDH1, but not ALDH-PB, mRNA stimulated retinoic acid synthesis in Xenopus embryos at the blastula stage. Thus our results indicate that Aldh1 can function in retinoic acid synthesis under physiological conditions, but that the closely related Aldh-pb does not share this property.  (+info)

Relationships within the aldehyde dehydrogenase extended family. (6/1111)

One hundred-forty-five full-length aldehyde dehydrogenase-related sequences were aligned to determine relationships within the aldehyde dehydrogenase (ALDH) extended family. The alignment reveals only four invariant residues: two glycines, a phenylalanine involved in NAD binding, and a glutamic acid that coordinates the nicotinamide ribose in certain E-NAD binary complex crystal structures, but which may also serve as a general base for the catalytic reaction. The cysteine that provides the catalytic thiol and its closest neighbor in space, an asparagine residue, are conserved in all ALDHs with demonstrated dehydrogenase activity. Sixteen residues are conserved in at least 95% of the sequences; 12 of these cluster into seven sequence motifs conserved in almost all ALDHs. These motifs cluster around the active site of the enzyme. Phylogenetic analysis of these ALDHs indicates at least 13 ALDH families, most of which have previously been identified but not grouped separately by alignment. ALDHs cluster into two main trunks of the phylogenetic tree. The largest, the "Class 3" trunk, contains mostly substrate-specific ALDH families, as well as the class 3 ALDH family itself. The other trunk, the "Class 1/2" trunk, contains mostly variable substrate ALDH families, including the class 1 and 2 ALDH families. Divergence of the substrate-specific ALDHs occurred earlier than the division between ALDHs with broad substrate specificities. A site on the World Wide Web has also been devoted to this alignment project.  (+info)

The loss in hydrophobic surface area resulting from a Leu to Val mutation at the N-terminus of the aldehyde dehydrogenase presequence prevents import of the protein into mitochondria. (7/1111)

An apparent conservative mutation, Leu to Val, at the second residue of the rat liver mitochondrial aldehyde dehydrogenase (ALDH) presequence resulted in a precursor protein that was not imported into mitochondria. Additional mutants were made to substitute various amino acids with nonpolar side chains for Leu2. The Ile, Phe, and Trp mutants were imported to an extent similar to that of the native precursor, but the Ala mutant was imported only about one-fourth as well. It was shown that the N-terminal methionine was removed from the L2V mutant in a reaction catalyzed by methionine aminopeptidase. The N-terminal methionine of native pALDH and the other mutant presequences was blocked, presumably by acetylation. Because of the difference in co-translational modification, the L2V mutant sustained a significant loss in the available hydrophobic surface of the presequence. Import competence was restored to the L2V mutant when it was translated using a system that did not remove Met1. The removal of an Arg-Gly-Pro helix linker segment (residues 11-14) from the L2V mutant, which shifted three leucine residues toward the N-terminus, also restored import competence. These results lead to the conclusion that a minimum amount of hydrophobic surface area near the N-termini of mitochondrial presequences is an essential property to determine their ability to be imported. As a result, both electrostatic and hydrophobic components must be considered when trying to understand the interactions between precursor proteins and proteins of the mitochondrial import apparatus.  (+info)

In vivo mitochondrial import. A comparison of leader sequence charge and structural relationships with the in vitro model resulting in evidence for co-translational import. (8/1111)

The positive charges and structural properties of the mitochondrial leader sequence of aldehyde dehydrogenase have been extensively studied in vitro. The results of these studies showed that increasing the helicity of this leader would compensate for reduced import from positive charge substitutions of arginine with glutamine or the insertion of negative charged residues made in the native leader. In this in vivo study, utilizing the green fluorescent protein (GFP) as a passenger protein, import results showed the opposite effect with respect to helicity, but the results from mutations made within the native leader sequence were consistent between the in vitro and in vivo experiments. Leader mutations that reduced the efficiency of import resulted in a cytosolic accumulation of a truncated GFP chimera that was fluorescent but devoid of a mitochondrial leader. The native leader efficiently imported before GFP could achieve a stable, import-incompetent structure, suggesting that import was coupled with translation. As a test for a co-translational mechanism, a chimera of GFP that contained the native leader of aldehyde dehydrogenase attached at the N terminus and a C-terminal endoplasmic reticulum targeting signal attached to the C terminus of GFP was constructed. This chimera was localized exclusively to mitochondria. The import result with the dual signal chimera provides support for a co-translational mitochondrial import pathway.  (+info)

Title:Mitochondrial Aldehyde Dehydrogenase, A Potential Drug Target for Protection of Heart and Brain from Ischemia/Reperfusion Injury. VOLUME: 15 ISSUE: 10. Author(s):Xiu-Ju Luo, Bin Liu, Qi-Lin Ma and Jun Peng. Affiliation:Department of Pharmacology, School of Pharmaceutical Sciences, Central South University. No.110 Xiang-Ya Road, Changsha, 410078, China.. Keywords:4-hydroxy-2-nonenal (4-HNE), Alda-1, aldehyde, ischemia/reperfusion injury, mitochondrial aldehyde dehydrogenase (ALDH2), oxidative stress.. Abstract:Mitochondrial aldehyde dehydrogenase (ALDH2) is an isoenzyme of aldehyde dehydrogenases (ALDH), a group of enzymes that are responsible for clearance of aldehydes in the body. In animal myocardial or cerebral ischemia/ reperfusion (I/R) models, accumulation of toxic aldehydes, such as 4-hydroxy-2-nonenal and malondialdehyde, is thought to be an important mechanism for myocardial and cerebral I/R injury. Among the isoenzymes of ALDH, ALDH2 is believed to play a major role in clearance ...
TY - JOUR. T1 - The mutation in the mitochondrial aldehyde dehydrogenase (ALDH2) gene responsible for alcohol-induced flushing increases turnover of the enzyme tetramers in a dominant fashion. AU - Xiao, Qing. AU - Weiner, Henry. AU - Crabb, David W.. PY - 1996/11/1. Y1 - 1996/11/1. N2 - Deficiency in mitochondrial aldehyde dehydrogenase (ALDH2), a tetrameric enzyme, results from inheriting one or two ALDH2*2 alleles. This allele encodes a protein subunit with a lysine for glutamate substitution at position 487 and is dominant over the wild-type allele, ALDH2*1. The ALDH2*2-encoded subunit (ALDH2K) reduces the activity of ALDH2 enzyme in cell lines expressing the wild-type subunit (ALDH2E). In addition to this effect on the enzyme activity, we now report that ALDH2*2 heterozygotes had lower levels of ALDH2 immunoreactive protein in autopsy liver samples. The half-lives of ALDH2 protein in HeLa cell lines expressing ALDH2*1, ALDH2*2, or both were determined by the rate of loss of immunoreactive ...
TY - JOUR. T1 - Sequence of the signal peptide for rat liver mitochondrial aldehyde dehydrogenase. AU - Farrés, J.. AU - Guan, K.L.. AU - Weiner, H.. PY - 1988/1/1. Y1 - 1988/1/1. U2 - http://dx.doi.org/10.1016/0006-291X(88)90740-1. DO - http://dx.doi.org/10.1016/0006-291X(88)90740-1. M3 - Article. VL - 150. SP - 1083. EP - 1087. IS - 3. ER - ...
High aldehyde dehydrogenase (ALDH) activity characterizes a subpopulation of cells with cancer stem cell (CSC) properties in several malignancies. To clarify whether ALDH can be used as a marker of cervical cancer stem cells (CCSCs), ALDH high and ALDH low cells were sorted from 4 cervical cancer cell lines and 5 primary tumor xenografts and examined for CSC characteristics. Here, we demonstrate that cervical cancer cells with high ALDH activity fulfill the functional criteria for CSCs: (1) ALDH high cells, unlike ALDH low cells, are highly tumorigenic in vivo; (2) ALDH high cells can give rise to both ALDH high and ALDH low cells in vitro and in vivo, thereby establishing a cellular hierarchy; and (3) ALDH high cells have enhanced self-renewal and differentiation potentials. Additionally, ALDH high cervical cancer cells are more resistant to cisplatin treatment than ALDH low cells. Finally, expression of the stem cell self-renewal-associated transcription factors OCT4, NANOG, KLF4 and BMI1 is ...
TY - JOUR. T1 - Widespread nonhematopoietic tissue distribution by transplanted human progenitor cells with high aldehyde dehydrogenase activity. AU - Hess, David A.. AU - Craft, Timothy P.. AU - Wirthlin, Louisa. AU - Hohm, Sarah. AU - Zhou, Ping. AU - Eades, William C.. AU - Creer, Michael H.. AU - Sands, Mark S.. AU - Nolta, Jan A.. PY - 2008/3/1. Y1 - 2008/3/1. N2 - Transplanted adult progenitor cells distribute to peripheral organs and can promote endogenous cellular repair in damaged tissues. However, development of cell-based regenerative therapies has been hindered by the lack of preclinical models to efficiently assess multiple organ distribution and difficulty defining human cells with regenerative function. After transplantation into β-glucuronidase (GUSB)-deficient NOD/SCID/mucopolysaccharidosis type VII mice, we characterized the distribution of lineage-depleted human umbilical cord blood-derived cells purified by selection using high aldehyde dehydrogenase (ALDH) activity with ...
TY - JOUR. T1 - Alcohol and aldehyde dehydrogenase gene polymorphisms influence susceptibility to esophageal cancer in Japanese alcoholics. AU - Yokoyama, Akira. AU - Muramatsu, Taro. AU - Omori, Tai. AU - Matsushita, Sachio. AU - Yoshimizu, Haruko. AU - Higuchi, Susumu. AU - Yokoyama, Tetsuji. AU - Maruyama, Katsuya. AU - Ishii, Hiromasa. PY - 1999/11. Y1 - 1999/11. N2 - Background: Studies have consistently demonstrated that inactive aldehyde dehydrogenase-2 (ALDH2), encoded by ALDH2*1/2*2, is closely associated with alcohol-related carcinogenesis. Recently, the contributions of alcohol dehydrogenase-2 (ADH2) polymorphism to alcoholism, esophageal cancer, and the flushing response have also been described. Methods: To determine the effects of ALDH2 and ADH2 genotypes in genetically based cancer susceptibility, lymphocyte DNA samples from 668 Japanese alcoholic men more than 40 years of age (91 with and 577 without esophageal cancer) were genotyped and the results were expressed as odds ratios ...
Preparations of sheep liver cytoplasmic aldehyde dehydrogenase obtained by published methods were found by analytical isoelectric focusing in the pH range 5-8 to contain 5-10% by weight of the mitochondrial aldehyde dehydrogenase. Under the conditions used the pI of the cytoplasmic enzyme is 6.2 and that of the mitochondrial enzyme 6.6. The mitochondrial enzyme can be removed from the preparation by selective precipitation of the cytoplasmic enzyme with (NH4)2SO4. Kinetic experiments and inhibition experiments with disulfiram show that the properties of the two sheep liver enzymes are so different that the presence of 10% mitochondrial enzyme in preparations of the cytoplasmic enzyme can introduce serious errors into results. Our results suggest that the presence of 10 microM-disulfiram in assays may completely inactivate the pure cytoplasmic enzyme. This result is in contrast with a previous report [kitson (1978) Biochem. U. 175, 83-90]. ...
TY - JOUR. T1 - Brief report. T2 - Aldehyde dehydrogenase activity is a biomarker of primitive normal human mammary luminal cells. AU - Eirew, Peter. AU - Kannan, Nagarajan. AU - Knapp, David J.H.F.. AU - Vaillant, François. AU - Emerman, Joanne T.. AU - Lindeman, Geoffrey J.. AU - Visvader, Jane E.. AU - Eaves, Connie J.. PY - 2012/2. Y1 - 2012/2. N2 - Elevated aldehyde dehydrogenase (ALDH) expression/activity has been identified as an important biomarker of primitive cells in various normal and malignant human tissues. Here we examined the level and type of ALDH expression and activity in different subsets of phenotypically and functionally defined normal human mammary cells. We find that the most primitive human mammary stem and progenitor cell types with bilineage differentiation potential show low ALDH activity but undergo a marked, selective, and transient upregulation of ALDH activity at the point of commitment to the luminal lineage. This mirrors a corresponding change in transcripts ...
The aldehyde dehydrogenase (ALDH) superfamily is composed of nicotinamide adenine dinucleotide (phosphate) (NAD(P)(+))-dependent enzymes that catalyze the oxidation of aldehydes to their corresponding carboxylic acids. To date, 24 ALDH gene families have been identified in the eukaryotic genome. In addition to aldehyde metabolizing capacity, ALDHs have additional catalytic (e.g. esterase and reductase) and non-catalytic activities. The latter include functioning as structural elements in the eye (crystallins) and as binding molecules to endobiotics and xenobiotics. Mutations in human ALDH genes and subsequent inborn errors in aldehyde metabolism are the molecular basis of several diseases. Most recently ALDH polymorphisms have been associated with gout and osteoporosis. Aldehyde dehydrogenase enzymes also play important roles in embryogenesis and development, neurotransmission, oxidative stress and cancer. This article serves as a comprehensive review of the current state of knowledge regarding ...
TY - JOUR. T1 - Order and disorder in mitochondrial aldehyde dehydrogenase. AU - Hurley, Thomas. AU - Perez-Miller, Samantha. AU - Breen, Heather. PY - 2001/1/30. Y1 - 2001/1/30. N2 - One of the most notable and currently unexplained features of the mitochondrial form of aldehyde dehydrogenase is its property of half-of-the-sites reactivity. An appropriate description of this phenomenon can be to consider this as the extreme example of negative cooperativity. This implies, therefore, that a pathway of communication must exist between active sites in order to convey the structural consequences of ligand binding. Data from four different structures of human ALDH2 collected during the past 2 years may shed some light on one possible pathway for the propagation of structural information. We recently published a 2.6 Å structure of a binary complex between ALDH2 and NAD+ in which the predominant conformation of the cofactor differed between different subunits in the structure. We now have three ...
TY - CONF. T1 - Expression and characterisation of human aldehyde dehydrogenase 10. T2 - implications for Sjögren-Larsson syndrome. AU - Lloyd, M. D.. AU - Boardman, K. D. E.. AU - Threadgill, M. D.. PY - 2006/9. Y1 - 2006/9. M3 - Other. ER - ...
Purpose To assess aldehyde dehydrogenase (ALDH) reflection in adult individual and murine submandibular gland (SMG) control cells and to determine the impact of ALDH3 account activation in SMG control cell enrichment. a story ALDH3 activator (Alda-89) or with automobile implemented by quantification of c-Kit+/Compact disc90+ SMG control cells and BrdUrd+ salispheres. Outcomes Even more than 99% of Compact disc34+ huSMG control cells tarnished positive for c-Kit, Compact disc90 and 70% colocalized with Compact disc44, Nestin. Likewise, 73.8% c-Kit+ mSMG control cells colocalized with Sca-1, whereas 80.7% with CD90. Functionally, these cells produced BrdUrd+ salispheres, which differentiated into acinar- and ductal-like buildings when cultured in 3D collagen. Both adult individual and murine SMG control cells demonstrated higher reflection of ALDH3 than in their nonCstem cells and 84% of these cells possess measurable ALDH1 activity. Alda-89 infusion in adult rodents considerably elevated ...
TY - JOUR. T1 - The effects of cephem antibiotics and related compounds on the aldehyde dehydrogenase in rat liver mitochondria. AU - Chiaki, Kamei. AU - Yukio, Sugimoto. AU - Kenji, Tasaka. PY - 1987/6/15. Y1 - 1987/6/15. N2 - The effects of cephem antibiotics and their related compounds on aldehyde dehydrogenase obtained from rat liver mitochondria were studied. A pH of 8.8 and reaction temperature 24° were the conditions for measurement of enzyme activity. The apparent Michaelis constant Km values for NAD, acetaldehyde and propionaldehyde were 3.8 × 10-5 M, 4.0 × 10-5 M and 2.5 × 10-5 M, respectively. Cefamandole, cefoperazone and cefmetazole, having a 1-methyl-5-thiotetrazol group at position 3 of the cephem ring, caused a relatively potent inhibition of aldehyde dehydrogenase. Cefmetazole and cefoperazone also showed a significant inhibition on highly purified yeast aldehyde dehydrogenase; the extent of inhibition on yeast enzyme was almost the same as that on rat mitochondrial aldehyde ...
Aldehyde dehydrogenases (ALDHs) play a major role in detoxification of aldehydes. High expression of ALDHs is a marker for stem cells of many organs including the lungs. A common polymorphism in ALDH2 gene (ALDH2*2) results in inactivation of the enzyme and is associated with alcohol flushing syndrome and increased risk for cardiovascular and Alzheimers diseases and some cancers. The effect of this ALDH2 polymorphism on the lung and its stem cells has not been thoroughly examined. We examined the association between the ALDH2*2 allele and lung function parameters in a population of healthy individuals. We also examined its association with the incidence of asthma and COPD in patient cohorts. We used the in vitro colony forming assay to detect the effect of the polymorphism on lung epithelial stem cells from both primary human surgical samples and Aldh2*2 transgenic (Tg) and Aldh2 −/− mice. Response to acute and chronic lung injuries was compared between wild type (WT),
Purified Recombinant Human ALDH2 293 Cell Lysate from Creative Biomart. Recombinant Human ALDH2 293 Cell Lysate can be used for research.
TY - JOUR. T1 - Alcohol and aldehyde dehydrogenase genotypes and drinking behavior in Japanese. AU - Higuchi, Susumu. AU - Matsushita, Sachio. AU - Muramatsu, Taro. AU - Murayama, Masanobu. AU - Hayashida, Motoi. PY - 1996/1/1. Y1 - 1996/1/1. N2 - The effects of the genotype of alcohol dehydrogenase-2 (ADH2) and mitochondrial aldehyde dehydrogenase (ALDH2) on drinking behavior were investigated in a population of 451 Japanese. Although the ALDH2*2 allele had a significant inhibitory effect on alcohol consumption, hence on drinking problems, the apparent association was not confirmed between ADH2 genotype and overall drinking patterns for either males or females. However, the frequency of the ADH2*2 allele was significantly lower in male Japanese classified as alcoholic on the basis of the Kurihama Alcoholism Screening Test than in nonalcoholic males. These results corroborate a previous study that revealed a significantly lower ADH2*2 allele frequency in hospitalized Japanese alcoholics than in ...
Aldehyde dehydrogenases (ALDHs) represent a group of enzymes that detoxify aldehydes by facilitating their oxidation to carboxylic acids, and have been shown to play roles in plant response to abiotic stresses. However, the comprehensive analysis of ALDH superfamily in soybean (Glycine max) has been limited. In present study, a total of 53 GmALDHs were identified in soybean, and grouped into 10 ALDH families according to the ALDH Gene Nomenclature Committee and phylogenetic analysis. These groupings were supported by their gene structures and conserved motifs. Soybean ALDH superfamily expanded mainly by whole genome duplication/segmental duplications. Gene network analysis identified 1146 putative co-functional genes of 51 GmALDHs. Gene Ontology (GO) enrichment analysis suggested the co-functional genes of these 51 GmALDHs were enriched (FDR < 1e-3) in the process of lipid metabolism, photosynthesis, proline catabolism, and small molecule catabolism. In addition, 22 co-functional genes of GmALDHs are
Disulfiram labeled with carbon-14 reacts specifically with human liver aldehyde dehydrogenase E1 with loss of catalytic activity and no incorporation of label. Carbon-14-labeled diethyldithiocarbamate is formed and the number of enzyme sulfhydryl groups decreases from 34 to 30 during this process.Activity is recovered by-mercaptoethanol but not by glutathione, the physiological reducing agent. ...
The precise phenotype and biology of acute myeloid leukemia stem cells remain controversial, in part because the gold standard immunodeficient mouse engraftment assay fails in a significant fraction of patients and identifies multiple cell-types in others. We sought to analyze the clinical utility of a novel assay for putative leukemia stem cells in a large prospective cohort. The leukemic clones most primitive hematopoietic cellular phenotype was prospectively identified in 109 newly-diagnosed acute myeloid leukemia patients, and correlated with clinical risk groups and outcomes. Most (80/109) patients harbored CD34+CD38- leukemia cells. The CD34+CD38- leukemia cells in 47 of the 80 patients displayed intermediate aldehyde dehydrogenase expression, while normal CD34+CD38- hematopoietic stem cells expressed high levels of aldehyde dehydrogenase. In the other 33/80 patients, the CD34+CD38- leukemia cells exhibited high aldehyde dehydrogenase activity, and most (28/33, 85%) harbored poor-risk ...
H1299, a NSCLC cell line with low endogenous ALDH expression was stably transduced with ALDH7A1. The gene and protein expression was evaluated using qRT-PCR and western blot, respectively. ALDH activity was measured with ALDEFLUOR assay. MTT assay was used to study cell proliferation, while cell migration was measured with scratch assay. ROS generation was detected using carboxy-H2DCFDA and dsDNA breaks were measured using the expression of phosphorylated H2AX. Five compounds with the highest binding affinity for ALDH7A1 from a virtual screening of 24,000 compounds were used to probe ALDH7A1 activity. ...
Creative Proteomics offer Aldehyde Dehydrogenase Activity Fluorometric Assay Kit. We are specialized in manufacturing Assay Kits.
PubMed journal article: Vascular bioactivation of nitroglycerin is catalyzed by cytosolic aldehyde dehydrogenase-2. Download Prime PubMed App to iPhone, iPad, or Android
The purpose of this comprehensive meta-analysis was to assess the association of aldehyde dehydrogenase (ALDH) expression with overall survival (OS) and disease-free survival (DFS)/progression-free survival (PFS) in ovarian cancer patients. Systematic searches of Pubmed databases was performed to identify relevant literature published before February 28, 2018. A total of 14 studies (13 articles) with 2210 ovarian cancer patients were pooled. All included studies were performed by using Immunohistochemistry (IHC) for detection of ALDH expression. Hazard ratio (HR) and 95% confidence interval (CI) were extracted from included studies to evaluate the correlation of ALDH expression with OS and DFS/PFS. High expression of ALDH was associated with worse OS (HR: 1.43; 95% CI: 1.18-1.73) and poor DFS/PFS (HR: 1.55, 95% CI: 1.12-2.14). No evidence of publication bias was observed in OS (Beggs test, P = 0.113; Eggers test, P = 0.355) and DFS/PFS (Beggs test, P = 0.655; Eggers test, P = 0.189) in ovarian
Melanoma is one of the most aggressive types of human cancer, characterized by enhanced heterogeneity and resistance to conventional therapy at advanced stages. We and others have previously shown that HEDGEHOG-GLI (HH-GLI) signaling is required for melanoma growth and for survival and expansion of melanoma-initiating cells (MICs). Recent reports indicate that HH-GLI signaling regulates a set of genes typically expressed in embryonic stem cells, including SOX2 (sex-determining region Y (SRY)-Box2). Here we address the function of SOX2 in human melanomas and MICs and its interaction with HH-GLI signaling. We find that SOX2 is highly expressed in melanoma stem cells. Knockdown of SOX2 sharply decreases self-renewal in melanoma spheres and in putative melanoma stem cells with high aldehyde dehydrogenase activity (ALDH(high)). Conversely, ectopic expression of SOX2 in melanoma cells enhances their self-renewal in vitro. SOX2 silencing also inhibits cell growth and induces apoptosis in melanoma ...
Aldehyde dehydrogenase (ALDH) activity is commonly used as a marker to identify cancer stem-like cells. The three ALDH1A isoforms have all been individually implicated in cancer stem-like cells and in chemoresistance; however, which isoform is preferentially expressed varies between cell lines. We sought to explore the structural determinants of ALDH1A isoform selectivity in a series of small-molecule inhibitors in support of research into the role of ALDH1A in cancer stem cells. An SAR campaign guided by a cocrystal structure of the HTS hit CM39 (7) with ALDH1A1 afforded first-in-class inhibitors of the ALDH1A subfamily with excellent selectivity over the homologous ALDH2 isoform. We also discovered the first reported modestly selective single isoform 1A2 and 1A3 inhibitors. Two compounds, 13g and 13h, depleted the CD133+ putative cancer stem cell pool, synergized with cisplatin, and achieved efficacious concentrations in vivo following IP administration. Compound 13h additionally synergized ...
As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
Alda-1 is a potent and selective Aldehyde Dehydrogenase-2 Agonist. Alda-1 reverses alcohol-induced hepatic steatosis and cell death in mice. Alda-1 inhibits atherosclerosis and attenuates hepatic steatosis in apolipoprotein E-knockout mice. Alda-1 reduces cerebral ischemia/reperfusion injury in rat through clearance of reactive aldehydes. Pharmacological activation of ALDH2 by Alda-1 reversed alcoholic steatosis and apoptosis through accelerating aldehyde clearance. This study indicates that ALDH2 is a promising molecular target and Alda-1 has therapeutic potential for treating alcoholic liver disease.
Aldehyde dehydrogenases (ALDHs) are highly expressed in the chemotherapy- and radiotherapy-resistant cell subpopulations of many different cancer types. Accordingly, the development of ALDH inhibitors may be the most direct approach to target these cell populations. However, inhibiting multiple ALDH …
Sci. Rep. Aldehyde dehydrogenase enzymes (ALDHs) are very common proteins involved in the oxidation of aliphatic and aromatic aldehydes to their corresponding…
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Background: Inherited human aldehyde dehydrogenase 2 (ALDH-2) deficiency reduces the risk for alcoholism. Kudzu plants and extracts have been used for 1,000 years in traditional Chinese medicine to treat alcoholism. Kudzu contains daidzin, which inhibits ALDH-2 and suppresses heavy drinking in rodents. Decreased drinking due to ALDH-2 inhibition is attributed to aversive properties of acetaldehyde accumulated during alcohol consumption. However, daidzin can reduce drinking in some rodents without necessarily increasing acetaldehyde. Therefore, a selective ALDH-2 inhibitor might affect other metabolic factors involved in regulating drinking.. Methods: Aldehyde dehydrogenase 2 inhibitors were synthesized based on the co-crystal structure of ALDH-2 and daidzin. We tested the efficacy of a highly selective reversible ALDH-2 inhibitor, CVT-10216, in models of moderate and high alcohol drinking rats. We studied 2-bottle choice and deprivation-induced drinking paradigms in Fawn Hooded (FH) rats, ...
Aldehyde dehydrogenases (ALDH, EC 1.2.1.3) are a group of enzymes that catalyze the oxidation (dehydrogenation) of aldehydes to carboxylic acids, an action also performed by xanthine oxidase (XO) and aldehyde oxidase (AO).. The aldehyde cinnamaldehyde commonly used as a food flavoring is rapidly oxidized by aldehyde dehydrogenases into the carboxylic acid cinnamic acid.. ...
Oxidizes medium and long chain saturated and unsaturated aldehydes (PubMed:17382292, PubMed:23721920). Metabolizes also benzaldehyde (PubMed:17382292). Low activity towards acetaldehyde and 3,4-dihydroxyphenylacetaldehyde (PubMed:17382292, PubMed:23721920). May not metabolize short chain aldehydes. Can use both NADP(+) and NAD(+) as electron acceptor (PubMed:17382292). May have a protective role against the cytotoxicity induced by lipid peroxidation (PubMed:17382292).
ALDH activity measured fluorimetrically using a high concentration of aliphatic aldehyde as substrate was studied in human glioblastomas grafted in nude mice. Compared with normal brain, ALDH...
TY - JOUR. T1 - Characterization of the molecular mechanisms underlying increased ischemic damage in the aldehyde dehydrogenase 2 genetic polymorphism using a human induced pluripotent stem cell model system. AU - Ebert, Antje D.. AU - Kodo, Kazuki. AU - Liang, Ping. AU - Wu, Haodi. AU - Huber, Bruno C.. AU - Riegler, Johannes. AU - Churko, Jared. AU - Lee, Jaecheol. AU - De Almeida, Patricia. AU - Lan, Feng. AU - Diecke, Sebastian. AU - Burridge, Paul W.. AU - Gold, Joseph D.. AU - Mochly-Rosen, Daria. AU - Wu, Joseph C.. PY - 2014/9/24. Y1 - 2014/9/24. N2 - Nearly 8% of the human population carries an inactivating point mutation in the gene that encodes the cardioprotective enzyme aldehyde dehydrogenase 2 (ALDH2). This genetic polymorphism (ALDH2∗2) is linked to more severe outcomes from ischemic heart damage and an increased risk of coronary artery disease (CAD), but the underlying molecular bases are unknown. We investigated the ALDH2∗2 mechanisms in a human model system of induced ...
15 min with 200 ml of 50 mM NH4HCO3 at RT. A volume of 200 ml of 100 acetonitrile was added to this solution and incubated for 15 min at room temperature. Solvent was removed and gel plugs were allowed to dry for 30 min at RT under a flow hood. Plugs were rehydrated with 20 ng/ml of modified trypsin (Promega, Madison, WI, USA) in 50 mM NH4HCO3 in a shaking incubator overnight at 37uC. Enough trypsin solution was added in order to completely submerge the gel plugs.sample was acquired for a total of ,2.5 min. MS/MS spectra were searched against the International Protein Index (IPI) database using SEQUEST with the following parameters: two trypsin miscleavages, fixed carbamidomethyl modification, variable methionine oxidation, parent tolerance 10 ppm, and fragment tolerance of 25 mmu or 0.01 Da. Results were filtered with the following criteria: Xcorr1.5, 2.0, 2.5, 3.0 for 1, 2, 3, and 4 charge states, respectively, Delta CN0.1, and P-value (protein and peptide) 0.01. IPI accession numbers were ...
Conditioned medium (CM) from clonal sub-populations of the pancreatic cancer cell line, MiaPaCa-2 with differing invasive abilities, were examined for their effect on in vitro invasion. Conditioned medium from Clone #3 (CM#3) strongly promoted invasion, while CM from Clone #8 (CM#8) inhibited invasion in vitro. 2D DIGE followed by MALDI-TOF MS analysis of CM#3 and CM#8 identified 41 proteins which were differentially regulated; 27 proteins were down-regulated and 14 proteins up-regulated in the invasion-promoting CM#3 when compared to CM#8. Western blotting analysis confirmed the down-regulated expression of gelsolin and the up-regulation of aldehyde dehydrogenase 1A1 in CM#3. Down-regulation of aldehyde dehydrogenase 1A1 in Clone #3 CM and gelsolin levels in Clone #8 CM by siRNA transfection revealed an important involvement of these proteins in promoting and inhibiting invasion in these pancreatic cancer cell lines. ...
TY - JOUR. T1 - Sex difference in hepatic microsomal aldehyde oxygenase activity in different strains of mice. AU - Watanabe, K.. AU - Matsunaga, T.. AU - Narimatsu, S.. AU - Yamamoto, I.. AU - Yoshimura, H.. PY - 1992/12/1. Y1 - 1992/12/1. N2 - Hepatic microsomal oxidation of 11-oxo-Δ8-tetrahydrocannabinol (11-oxo- Δ8-THC) and 9-anthraldehyde (9-AA) to the corresponding carboxylic acids was investigated using six strains of male and female mice (ddN, ddY, C57BL, DBA, C3H and ICR). No significant sex difference was observed in the activity toward 11-oxo-Δ8-THC except for ICR, whereas the activity toward 9-AA was significantly higher in female than in male of ddN, C57BL, DBA and C3H mice. The present study suggests that female specific form(s) of cytochrome P450 may be responsible at least in part for the microsomal oxidation of 9-AA, but not that of 11-oxo-Δ8-THC.. AB - Hepatic microsomal oxidation of 11-oxo-Δ8-tetrahydrocannabinol (11-oxo- Δ8-THC) and 9-anthraldehyde (9-AA) to the ...
NCT 501 (CAS: 1802088-50-1)is a potent and selective theophylline-based inhibitor of aldehyde dehydrogenase 1A1 (ALDH1A1), inhibits hALDH1A1 with IC50 of 40 nM, typically shows better selectivity over other ALDH isozymes and other dehydrogenases (hALDH1B1
Improved aldehyde dehydrogenase 1 (ALDH1) activity continues to be within the stem cell populations of leukemia plus some solid tumors including non-small-cell lung cancer (NSCLC). not really in SCC individuals. High manifestation of both and mRNA had not been found to become correlated to Operating-system in every NSCLC individuals. These results highly support that ALDH1A1 mRNA in NSCLC is definitely connected with better prognosis. Furthermore, our current research also facilitates that ALDH1A2 and ALDH1B1 may be main contributors towards the ALDH1 activity in NSCLC, since high manifestation of and mRNA was discovered to be considerably correlated to worser Operating-system in every NSCLC individuals. Predicated on our research, ALDH1A2 and ALDH1B1 may be superb potential drug focuses on for NSCLC individuals. members mRNA manifestation to relapse-free success. Currently, they possess breast malignancy,15 lung malignancy,17 ovarian malignancy,28 and gastric malignancy database. NSCLC ...
Semantic Scholar extracted view of Aldehyde dehydrogenase activity and level of dopamine in certain sections of the brain of rats preferring and refusing ethanol by Kharchenko Nk
Application of stem cell biology to breast cancer research has been limited by the lack of simple methods for identification and isolation of normal and malignant stem cells. Utilizing in vitro and in vivo experimental systems, we show that normal and cancer human mammary epithelial cells with increased aldehyde dehydrogenase activity (ALDH) have stem/progenitor properties. These cells contain the subpopulation of normal breast epithelium with the broadest lineage differentiation potential and greatest growth capacity in a xenotransplant model. In breast carcinomas, high ALDH activity identifies the tumorigenic cell fraction, capable of self-renewal and of generating tumors that recapitulate the heterogeneity of the parental tumor. In a series of 577 breast carcinomas, expression of ALDH1 detected by immunostaining correlated with poor prognosis. These findings offer an important new tool for the study of normal and malignant breast stem cells and facilitate the clinical application of stem cell ...
Hematopoiesis is sustained throughout adult life by balanced self-renewal and differentiation divisions of multipotent cells. The restriction of these cells to specific lineages takes place in an orderly sequence that spans several cell generations, thus creating hierarchies of daughter cells with distinct properties. Hematopoietic stem cells (HSC) represent the cells that head up this hierarchy and are thus able to regenerate and sustain the long term production of all blood cell types when transplanted at low numbers into suitable hosts. Quantification of HSC is achieved by coupling the end-points of donor-derived lymphoid and myeloid cell output to long term limiting dilution transplantation assays.1 In mice, phenotypically distinct cells with different repopulating activities in vivo have been described.2-7 Some short-term repopulating cells (STRC) lack the extensive self-renewal ability of HSC but retain a broad lympho-myeloid differentiation repertoire. Others also show partial restriction ...
The root cause is a genetic predisposition to acetaldehyde build-up that is most commonly observed in people of Asian decent, hence it being colloquially termed Asian flush despite it not being exclusive to Asians.. Acetaldehyde is a toxic by-product of alcohol metabolism that is usually broken down by enzymes in our body in order to stop it from accumulating and causing a toxic reaction - i.e. the various symptoms of Asian flush. All that is required is one of more specific gene variants to be present for acetaldehyde build-up to occur.. One gene variant, observable in approximately 50% of Asian people, is called the mitochondrial ALDH2 allele, which results in a deficient aldehyde dehydrogenase enzyme that works at about 10% of its usual capacity. This enzyme is normally responsible for preventing acetaldehyde build up by catalyzing its conversion into non-toxic acetic acid.. In addition to this, and contrary to common belief, a study confirmed that 80% of Asians and nearly all Japanese, ...
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ALDH5A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ALDH5A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ALDH5A1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ALDH5A1 bound in the initial step. The color development is stopped and the intensity of the color is measured. ...
Rabbit monoclonal antibody raised against recombinant human ALDH1A1. Recombinant protein corresponding to human ALDH1A1. (MAB21821) - Products - Abnova
Aldehyde dehydrogenases in rat brain: subcellular distribution, properties and involvement in acetaldehyde and dopamine metabolism ...
ALDH1A2 - ALDH1A2 (untagged)-Human aldehyde dehydrogenase 1 family, member A2 (ALDH1A2), transcript variant 2 available for purchase from OriGene - Your Gene Company.
Looking for online definition of Aldehyde Dehydrogenase 5 Family, Member A1 in the Medical Dictionary? Aldehyde Dehydrogenase 5 Family, Member A1 explanation free. What is Aldehyde Dehydrogenase 5 Family, Member A1? Meaning of Aldehyde Dehydrogenase 5 Family, Member A1 medical term. What does Aldehyde Dehydrogenase 5 Family, Member A1 mean?
Although aldehyde dehydrogenase (ALDH) from sheep liver cytosol has a broad specificity, it will not oxidize the aldehyde group of glyoxylic acid which is in fact an inhibitor of the enzyme. The inhibition pattern is non-linear but competitive at high propionaldehyde concentrations (2-20 mM); however, a simple non-competitive pattern is observed at low (less than 100 microM) propionaldehyde concentrations (Ki = 1.6 mM). The esterase activity was unaffected by glyoxylic acid in the absence of NAD+ but a simple competitive inhibition pattern (Ki = 2.5 mM) was observed with respect to 4-nitrophenyl acetate in the presence of NAD+. The data require a two-site model in which ester and aldehyde binding sites are distinct but with a second propionaldehyde molecule, and glyoxylic acid, binding at or near the ester binding site. Consistent with this model is the fact that chloral hydrate was a non-competitive inhibitor of the esterase activity in the presence of NAD+ but a competitive inhibitor in its ...
K00128 ALDH; aldehyde dehydrogenase (NAD+) [EC:1.2.1.3] K00128 ALDH; aldehyde dehydrogenase (NAD+) [EC:1.2.1.3] K00128 ALDH; aldehyde dehydrogenase (NAD+) [EC:1.2.1.3] K00128 ALDH; aldehyde dehydrogenase (NAD+) [EC:1.2.1.3] K00128 ALDH; aldehyde dehydrogenase (NAD+) [EC:1.2.1.3] K00128 ALDH; aldehyde dehydrogenase (NAD+) [EC:1.2.1.3] K14085 ALDH7A1; aldehyde dehydrogenase family 7 member A1 [EC:1.2.1.31 1.2.1.8 1.2.1.3 ...
TY - JOUR. T1 - Association of genetically determined aldehyde dehydrogenase 2 activity with diabetic complications in relation to alcohol consumption in Japanese patients with type 2 diabetes mellitus. T2 - The fukuoka diabetes registry. AU - Idewaki, Yasuhiro. AU - Iwase, Masanori. AU - Fujii, Hiroki. AU - Ohkuma, Toshiaki. AU - Ide, Hitoshi. AU - Kaizu, Shinako. AU - Jodai, Tamaki. AU - Kikuchi, Yohei. AU - Hirano, Atsushi. AU - Nakamura, Udai. AU - Kubo, Michiaki. AU - Kitazono, Takanari. N1 - Publisher Copyright: © 2015 Idewaki et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Copyright: Copyright 2016 Elsevier B.V., All rights reserved.. PY - 2015/11/1. Y1 - 2015/11/1. N2 - Aldehyde dehydrogenase 2 (ALDH2) detoxifies aldehyde produced during ethanol metabolism and oxidative stress. A genetic defect ...
TY - JOUR. T1 - Oxygen-tolerant coenzyme A-acylating aldehyde dehydrogenase facilitates efficient photosynthetic n-butanol biosynthesis in cyanobacteria. AU - Lan, Ethan I.. AU - Ro, Soo Y.. AU - Liao, James C.. PY - 2013/9/1. Y1 - 2013/9/1. N2 - Metabolic engineering of photosynthetic microorganisms such as cyanobacteria for the production of fuels or chemicals is challenging, particularly when the pathway involves oxygen-sensitive enzymes. We have previously designed a coenzyme A (CoA) dependent n-butanol biosynthesis pathway tailored to the metabolic physiology of the cyanobacterium Synechococcus elongatus PCC 7942 by incorporating an ATP driving force and a kinetically irreversible trap. However, one of the enzymes involved, CoA-acylating butyraldehyde dehydrogenase (Bldh) is oxygen sensitive, therefore hindering efficient n-butanol synthesis in cyanobacteria. To overcome this obstacle of n-butanol biosynthesis, we characterized six oxygen tolerant CoA-acylating aldehyde dehydrogenases ...
Author: Kliefoth, Michael et al.; Genre: Journal Article; Published in Print: 2012-02; Keywords: Methanosarcina acetivoran; Carbon monoxide; Acclimation; Aldehyde dehydrogenase; Sensing; Regulation; Title: Genetic analysis of MA4079, an aldehyde dehydrogenase homolog, in Methanosarcina acetivorans
TY - JOUR. T1 - Retroviral-infection increases tumorigenic potential of MDA-MB-231 breast carcinoma cells by expanding an aldehyde dehydrogenase (ALDH1) positive stem-cell like population. AU - Wegman-Points, Lauren J.. AU - Teoh-Fitzgerald, Melissa L.T.. AU - Mao, Gaowei. AU - Zhu, Yueming. AU - Fath, Melissa A.. AU - Spitz, Douglas R.. AU - Domann, Frederick E.. PY - 2014. Y1 - 2014. N2 - Retroviral transformation has been associated with pro-proliferative oncogenic signaling in human cells. The current study demonstrates that transduction of human breast carcinoma cells (MDA-MB231) with LXSN and QCXIP retroviral vectors causes significant increases in growth rate, clonogenic fraction, and aldehyde dehydrogenase-1 positive cells (ALDH1+), which is associated with increased steady-state levels of cancer stem cell populations. Furthermore, this retroviral-induced enhancement of cancer cell growth in vitro was also accompanied by a significant increase in xenograft tumor growth rate in vivo. The ...
The ALDH3A2 gene is a member of the aldehyde dehydrogenase (ALDH) gene family. Learn about this gene and related health conditions.
A single point mutation in ALDH2-gene provides conclusive evidence for a causal relationship between acetaldehyde and upper GI tract cancer. Mutation results in deficient activity of the main acetaldehyde metabolizing enzyme mitochondrial aldehyde dehydrogenase (ALDH2). When drinking alcohol, ALDH2-deficients are exposed via saliva to 2-3 times and via gastric juice to 5-6 times higher acetaldehyde concentrations than individuals with active ALDH2-enzyme. Parallel to increased local acetaldehyde exposure, the risk of ALDH2-deficient alcohol drinkers for oral, pharyngeal, esophageal and gastric cancer is many fold compared to alcohol drinking ALDH2-actives. Based on strong gene-epidemiological and gene-biochemical evidence, the International Agency for Research on Cancer (IARC) has reclassified acetaldehyde associated with the consumption of alcoholic beverages as a group 1 human carcinogen. Equal gene mutation based human cancer model is not available for any other of the 118 group 1 human ...
It seems that benomyl triggers a series of events leading to Parkinsons disease onset. It is assumed that the chemical interferes with an enzyme called ALDH (aldehyde dehydrogenase). In this way, aldehyde dehydrogenase can not prevent accumulation of DOPAL, a toxic substance that has harmful effect on neurons and which greatly increases the risk of developing Parkinsons disease. Benomyl is a substance that has been widely used in the U.S. until it was banned in 2001 because it was found to have toxic effects on health. It seems that this toxic can cause brain malformations, tumors of the liver, reproductive abnormalities, etc.. Furthermore, the destructive effect of benomyl on dopaminergic neurons has been shown in cell culture experiments. Now neuroscientists at UCLA believe that this cascade of events occurs in all patients with Parkinsons disease. If they were create a drug to protect aldehyde dehydrogenase enzyme activity, they could treat or prevent Parkinsons disease. Parkinsons ...
Aldh1l2 (untagged) - Mouse aldehyde dehydrogenase 1 family, member L2 (Aldh1l2), nuclear gene encoding mitochondrial protein, (10ug), 10 µg.
In models of triple-negative breast cancer (TNBC), it has recently been shown that the epidermal growth factor receptor (EGFR) pathway is up-regulated in the aldehyde dehydrogenase 1 (ALDH1)-positive cancer stem cell fraction. Because high-grade serous ovarian carcinoma (HGSC) reveals strong molecular similarities to TNBC, we aimed to investigate the potential link between ALDH1 and EGFR in this entity. Expression of ALDH1 was investigated in 131 primary HGSCs using immunohistochemistry. Expression data were correlated with EGFR expression as well as with clinicopathologic parameters and survival. Forty-two carcinomas (32.1%) were positive for ALDH1 protein expression. Data on EGFR expression were available for 112 tumors. In these cases ALDH1 was significantly linked to EGFR expression (P , .0001). ALDH1 positivity was a significant negative prognostic factor for overall survival both in univariate (P = .010) and in multivariate survival analyses (P = .041). Tumors that were positive for both ...
This protein belongs to the aldehyde dehydrogenase family of proteins. This gene encodes a mitochondrial NAD(+)-dependent succinic semialdehyde dehydrogenase. A deficiency of this enzyme, known as 4-hydroxybutyricaciduria, is a rare inborn error in the metabolism of the neurotransmitter 4-aminobutyric acid (GABA). In response to the defect, physiologic fluids from patients accumulate GHB, a compound with numerous neuromodulatory properties. Two transcript variants encoding distinct isoforms have been identified for this gene.
In the liver, ethanol is predominantly metabolised by alcohol dehydrogenase (ADH) and CYP 2E1, resulting in acetaldehyde (AA) formation. AA, the extremely toxic first intermediate of ethanol metabolism, binds rapidly to cellular proteins and also possibly to DNA. These AA adducts represent neoantigens leading to the formation of specific antibodies.26 AA has mutagenic and carcinogenic properties leading to metaplasia, inhibition of DNA repair,27 sister chromatid exchanges,28 stimulation of apoptosis, and enhanced cell injury associated with hyperregeneration.29 According to the International Agency for Research on Cancer, there is sufficient evidence to identify AA as a carcinogen in animals.. Ethanol is metabolised by the successive action of ADH and aldehyde dehydrogenase (ALDH). For both ADH and ALDH, genetic polymorphisms have been described that influence the rate of conversion of ethanol to AA and of the latter to acetate.30 It has been consistently reported that ALDH2 is the most ...
Primary intrinsic deuterium and 13C isotope effects have been determined for liver (LADH) and yeast (YADH) alcohol dehydrogenases with benzyl alcohol as substrate and for yeast aldehyde dehydrogenase (ALDH) with benzaldehyde as substrate. These values have also been determined for LADH as a function of changing nucleotide substrate. As the redox potential of the nucleotide changes from -0.320 V with NAD to -0.258 V with acetylpyridine-NAD, the product of primary and secondary deuterium isotope effects rises from 4 toward 6.5, while the primary 13C isotope effect drops from 1.025 to 1.012, suggesting a trend from a late transition state with NAD to one that is more symmetrical. The values of Dk (again the product of primary and secondary isotope effects) and 13k for YADH with NAD are 7 and 1.023, suggesting for this very slow reaction a more stretched, and thus symmetrical, transition state. With ALDH and NAD, the primary 13C isotope effect on the hydride transfer step lies in the range 1.3-1.6%, and the
Ruth, like many Asian Americans, has at times been reluctant to drink wine because of her tendency to turn a deep shade of red after even just half a glass of red or white.. The cause of this wine flush has to do with how many Asians metabolize alchohol. Alchohol is absorbed through the stomach and small intestine. About 10 percent is eliminated by the kidneys, lungs, and sweat glands, but the rest is dealt with in the liver by two enzymes: alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH). I dont know enough about the bodys chemistry to know how these two enzymes actually manage to break down the alchohol, but its enough to know that they do.. Its this second enzyme (ALDH) that is missing (or low) in up to 50 percent of Asian people, and is not present at all in most Native Americans and Inuits. People with less ALDH will often flush and sweat after drinking alcohol, and if their quantities of the enzyme are quite low, they may also become ill after drinking even small amounts ...
TY - JOUR. T1 - Alterations in cancer stem-cell marker CD44 expression predict oncologic outcome in soft-tissue sarcomas. AU - Henderson, Timothy. AU - Chen, Mingyi. AU - Darrow, Morgan. AU - Li, Chin-Shang. AU - Chiu, Chi Lu. AU - Monjazeb, Arta M. AU - Murphy, William J. AU - Canter, Robert J. PY - 2018/3/1. Y1 - 2018/3/1. N2 - Background Cancer stem cells (CSCs) have been shown to resist chemotherapy and promote metastasis after cytotoxic therapies. We sought to determine if the expression of CSC markers (aldehyde dehydrogenase [ALDH], CD44, and epidermal growth factor receptor [EGFR]) predicted outcomes in soft-tissue sarcoma (STS) patients. Methods We queried an institutional database of 23 STS patients and evaluated immunohistochemical expression of CSC markers ALDH, CD44, and EGFR. The Cancer Genome Atlas (TCGA) was also queried for STS clinical and genomic data. Disease-specific (DSS) and overall survival (OS) were assessed by univariate and Kaplan-Meier analysis. Results Of the 23 ...
Title:Contribution of ALDH2 Polymorphism to Alcoholism-Associated Hypertension. VOLUME: 8 ISSUE: 3. Author(s):Nan Hu, Yingmei Zhang, Sreejayan Nair, Bruce W. Culver and Jun Ren. Affiliation:Center for Cardiovascular Research and Alternative Medicine, University of Wyoming College of Health Sciences, Laramie, WY 82071, USA.. Keywords:Acetaldehyde, alcohol, cardiovascular complications, hypertension, metabolism, mitochondrial aldehyde dehydrogenase.. Abstract:Chronic alcohol intake is considered as an independent lifestyle factor that may influence the risk of a number of cardiovascular anomalies including hypertension. In healthy adults, binge drinking and chronic alcohol ingestion lead to the onset and development of hypertension although the precise mechanism(s) remains obscure. Although oxidative stress and endothelial injury have been postulated to play a major contributing role to alcoholism-induced hypertension, recent evidence depicted a rather unique role for the genotype of the ...
Alcohol dehydrogenase 1B is an enzyme that in humans is encoded by the ADH1B gene. The protein encoded by this gene is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This encoded protein, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. The human gene is located on chromosome 4 in 4q22. Previously ADH1B was called ADH2. There are more genes in the family of alcohol and aldehyde dehydrogenase genes. These genes are now referred to as ADH1A, ADH1C, and ADH4, ADH5, ADH6 and ADH7. A single nucleotide polymorphism (SNP) in ADH1B is rs1229984, that changes arginine to histidine at residue 47. The ...
Summary is not available for the mouse gene. This summary is for the human ortholog.] This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of East Asians have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among East Asians than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for ...
The tubules of the kidney display a remarkable capacity for self-renewal on damage. Whether this regeneration is mediated by dedifferentiating surviving cells or, as recently suggested, by stem cells has not been unequivocally settled. Herein, we demonstrate that aldehyde dehydrogenase (ALDH) activi …
Catalyzes the oxidation 4-aminobutanal (gamma-aminobutyraldehyde) to 4-aminobutanoate (gamma-aminobutyrate or GABA) (PubMed:3510672, PubMed:16023116). This is the second step in one of two pathways for putrescine degradation, where putrescine is converted into 4-aminobutanoate via 4-aminobutanal, which allows E.coli to grow on putrescine as the sole nitrogen source (PubMed:3510672, PubMed:22636776). Also functions as a 5-aminopentanal dehydrogenase in a a L-lysine degradation pathway to succinate that proceeds via cadaverine, glutarate and L-2-hydroxyglutarate (PubMed:30498244). Can also oxidize n-alkyl medium-chain aldehydes, but with a lower catalytic efficiency (PubMed:15381418, PubMed:16023116).
This is from a British nurse of my acquaintance - she contests the paragraph in the Toxicity section dealing with drinking ethanol to avoid methanol poisoning. I asked her because I thought that that paragraph could be useful to remember for first aid purposes, but I figured I should verify it with a medical professional first. Can SOMEBODY, PLEASE, verify one over the other? I know Wikipedia is not a doctor, but for Chrissakes my age group will read this and think, Oh, Johnnys dying of Listerine ingestion, well just get some Jager into him... --207.216.10.77 08:28, 31 October 2006 (UTC). Ethanol is toxic, and the body begins to dispose of it immediately upon its consumption. Over 90% of it is processed by the liver. In the liver, the alcohol dehydrogenase enzyme converts ethanol into acetaldehyde, which is itself toxic. Acetaldehyde is destroyed almost immediately by the aldehyde dehydrogenase enzyme, which converts it to acetate ions.. The hydrogen atoms represented by these equations are ...
The oxazaphosphorines cyclophosphamide, ifosfamide and trofosfamide remain a clinically useful class of anticancer drugs with substantial antitumour activity against a variety of solid tumors and hematological malignancies. A major limitation to their use is tumour resistance, which is due to multiple mechanisms that include increased DNA repair, increased cellular thiol levels, glutathione S-transferase and aldehyde dehydrogenase activities, and altered cell-death response to DNA damage. These mechanisms have been recently re-examined with the aid of sensitive analytical techniques, high-throughput proteomic and genomic approaches, and powerful pharmacogenetic tools. Oxazaphosphorine resistance, together with dose-limiting toxicity (mainly neutropenia and neurotoxicity), significantly hinders chemotherapy in patients, and hence, there is compelling need to find ways to overcome it. Four major approaches are currently being explored in preclinical models, some also in patients: combination with ...
Retinoic acid (RA), the active metabolite of vitamin A, broadly regulates gene expression. The RA signaling pathway plays an essential role in embryonic development, including the development of the body axis, eye, brain, and heart (Ghyselinck & Duester, 2019). One of the key enzymes in the biosynthesis of RA is aldehyde dehydrogenase 1 family member A3 (ALDH1A3). ALDH1A3 converts retinaldehyde to retinoic acid and it is expressed early in forebrain development (McCaffery & Drager, 1994). Several mutations in ALDH1A3 have been implicated in patients with autosomal recessive microphthalmia and other neurological disorders (Fares-Taie et al., 2013; Roos et al., 2014). However, current animal models of ALDH1A3 have large truncations of the protein. Direct evidence of the effects of missense variants on ALDH1A3 protein activity has not yet been obtained.. In this study, we aimed to determine the functional consequence of ALDH1A3(C174Y) missense variants implicated in patients (Roos et al., 2014). ...
Inflammatory Breast Cancer (IBC) is a rare, highly aggressive form of cancer that is frequently locally advanced or metastasized at the time of diagnosis. The Triple-Negative subtype of IBC (TN-IBC), in particular, is characterized by very poor overall survival. TN-IBC lacks targeted therapies and is primarily treated with radiation or chemotherapy, which are inefficient. Recently, we identified the cell surface transmembrane ADAM8 (A Disintegrin and Metalloproteinase) protein as a driver of Triple-Negative Breast Cancer (TNBC) growth and metastasis via its Metalloproteinase (MP) and Disintegrin (DI) domains, respectively. In in vivo proof-of-concept experiments with a prototype reagent, we demonstrated that simultaneous, antibody-based, targeting of the ADAM8 MP and DI domains is an effective therapeutic approach for TNBC (Romagnoli et al., EMBO Mol. Med. 6:278, 2014).. The aggressive behavior of IBC cells has been attributed to a stem-like cancer cell compartment with high ALDH activity ...
131716PRTInfluenza A virus 1Met Glu Asp Phe Val Arg Gln Cys Phe Asn Pro Met Ile Val Glu Leu1 5 10 15Ala Glu Lys Thr Met Lys Glu Tyr Gly Glu Asp Leu Lys Ile Glu Thr 20 25 30Asn Lys Phe Ala Ala Ile Cys Thr His Leu Glu Val Cys Phe Met Tyr 35 40 45Ser Asp Phe His Phe Ile Asn Glu Gln Gly Glu Ser Ile Ile Val Glu 50 55 60Leu Gly Asp Pro Asn Ala Leu Leu Lys His Arg Phe Glu Ile Ile Glu65 70 75 80Gly Arg Asp Arg Thr Met Ala Trp Thr Val Val Asn Ser Ile Cys Asn 85 90 95Thr Thr Gly Ala Glu Lys Pro Lys Phe Leu Pro Asp Leu Tyr Asp Tyr 100 105 110Lys Glu Asn Arg Phe Ile Glu Ile Gly Val Thr Arg Arg Glu Val His 115 120 125Ile Tyr Tyr Leu Glu Lys Ala Asn Lys Ile Lys Ser Glu Lys Thr His 130 135 140Ile His Ile Phe Ser Phe Thr Gly Glu Glu Met Ala Thr Lys Ala Asp145 150 155 160Tyr Thr Leu Asp Glu Glu Ser Arg Ala Arg Ile Lys Thr Arg Leu Phe 165 170 175Thr Ile Arg Gln Glu Met Ala Ser Arg Gly Leu Trp Asp Ser Phe Arg 180 185 190Gln Ser Glu Arg Gly Glu Glu Thr Ile Glu Glu Arg Phe Glu Ile Thr 195 200 205Gly Thr Met Arg Lys ...
COLON STEM CELLS ASSOCIATED WITH COLITISAND COLORECTAL CANCER AND METHODS OF USE - The disclosure provides methods of isolating and propagating self-renewing colonic stem/progenitor cells (CS/PCs) that express aldehyde dehydrogenase (ALDH1), from colon cancer and colitis tissues, as well as from normal colon tissue, methods of identifying agents for modulating the proliferative status of such cells, an methods of screening patients having colitis for an increased risk of colorectal cancer. Novel methods of adherent cell culture propagation of CS/PC involving use of colon-specific fibroblastic stromal cells (CFSt) {i.e. the niche cells) as support cells (e.g., feeder cells). The present disclosure encompasses an isolated mammalian pluripotent colon epithelial stem/progenitor cell (CS/PC), or a population of said cells, where each CS/PC may comprise a detectable marker, where the detectable marker is aldehyde dehydrogenase 1 (ALDH1), and where the isolated population of mammalian pluripotent ...
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Streptomyces aureofaciens putative aldehyde dehydrogenase AldA (AAD23400)-like. Putative aldehyde dehydrogenase, AldA, from Streptomyces aureofaciens (locus AAD23400) and other similar sequences are present in this CD. ...
Sigma-Aldrich offers abstracts and full-text articles by [Aroa López-Sánchez, Belén Floriano, Eloisa Andújar, Maria José Hernáez, Eduardo Santero].
Autor: Macedo , Bárbara Beatriz Pinto (1994-)Autor secundário: Sarmento, Ana Bela Ribeiro;Gonçalves , Ana Cristina;Amado, FranciscoAutor Institucional (Secundário): Universidade de Aveiro. Departamento de Química.Língua: Inglês.País: Portugal.Publicação: Aveiro : B. Macedo, 2017Descrição: 92 p., pag. var. : il. colorDescrição: 1 CD-ROMTítulo paralelo: Aldeído desidrogenases como biomarcadores em Neoplasias MieloidesAssunto : Bioquímica -- Teses de mestrado // Aldeídos // Desidrogenases // Células estaminais hematopoiéticas // Tumores // Leucemia // DiagnósticoCDU: 576.38:616.155(043)Recursos em linha:Formato digital ...
CONCLUSIONS Inactive ALDH2 genotype was frequently observed in DM-Mt3243. It suggests that DM-Mt3243 is associated with ALDH2 inactivity. We speculate the trait of acetaldehyde accumulation on ALDH2 inactivity may favor mitochondrial DNA abnormalities, thereby worsening ATP production and impairing insulin secretion. In addition, the interaction of ALDH1 and ALDH2 may alter the retinoid metabolism in the pancreas, thereby influencing insulin secretion and precipitating diabetes. Thus, this association of ALDH2 genotype with DM-Mt3243 provides insight into the etiology of diabetes in the mitochondrial diseases.. ...
TY - JOUR. T1 - Aldehydes in hydrothermal solution. T2 - Standard partial molal thermodynamic properties and relative stabilities at high temperatures and pressures. AU - Schulte, Mitchell D.. AU - Shock, Everett L.. PY - 1993/8. Y1 - 1993/8. N2 - Aldehydes are common in a variety of geologic environments and are derived from a number of sources, both natural and anthropogenic. Experimental data for aqueous aldehydes were taken from the literature and used, along with parameters for the revised Helgeson-Kirkham-Flowers (HKF) equations of state, to estimate standard partial molal thermodynamic data for aqueous straight-chain alkyl aldehydes at high temperatures and pressures. Examples of calculations involving aldehydes in geological environments are given, and the stability of aldehydes relative to carboxylic acids is evaluated. These calculations indicate that aldehydes may be intermediates in the formation of carboxylic acids from hydrocarbons in sedimentary basin brines and hydrothermal ...
Aldehydes Catalog with China Aldehydes Products, Aldehydes Suppliers, Aldehydes Manufacturers, and Aldehydes Exporters provided by ChemNet
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l Regarding Alexandra Petris June 7 blog post Twitter makes fools of us all:. Twitter doesnt make fools out of us. People who are already foolish, immature and full of asinine urges make fools out of themselves. Rep. Anthony Weiner tried to blame everyone else for his failings. When that didnt work, he finally confessed and took blame for his actions, as he should have days ago. What Ms. Petri has attempted to do is to provide Mr. Weiner, and others of his ilk, with another convenient out: Twitter made me do it!. Elaine J. Lancaster, Newburg, Md.. sigok. Hi John,. I earlier wrote from my cell phone - hard to type. Answers to verifications: I am the author. I did not cut or paste or copy this. My real name is Elaine J. Lancaster. I have only sent this letter to the Post. No one or organization asked me to write the letter. I am an outraged reader - thats my involvement. I live in Newburg, MD. Day and evening phone # already provided. ...
Background]Aldehyde dehydrogenase 1 (ALDH1) has been identified as a breast cancer stem cell marker. The clinical significance of ALDH1 as a chemo-resistant and prognostic indicator has been reported recently. However, the analysis according to each intrinsic subtype was not reported.. [Aims] To investigate the impact of ALDH1 on chemo-resistance and prognosis according to intrinsic subtypes in invasive breast cancers.. [Methods]. 1) Patients and tumor specimens; A total of 653 primary breast cancer patients were enrolled in this study from 2004 to 2013 at the Yokohama City University Medical Center in Japan. We performed immunohistochemical analyses using paraffin-embedded core needle biopsy sections prior to the treatment.. 2) Correlation of ALDH1 with clinicopathological factors;. Analyses were performed to investigate association of ALDH1 expression with other biomarkers and clinicopathological factors in breast cancers. Age, histologic type, tumor size, nodal status, ER/PgR/HER2 status, ...
Acetaldehyde may be the culprit behind hangovers, according to new research from Japan. ... The problem many East Asians have in drinking alcohol is that their livers have a mutant form of the enzyme aldehyde dehydrogenase-2 (ALDH2), which in other people eliminates the acetaldehyde formed by ethanol metabolism, but often fails to do its job properly in East Asians, which means they suffer worse hangovers as this toxic compound stays in their system at higher concentrations than it would otherwise do so ...
Aldehyde dehydrogenase 3I1; Involved in oxidative stress tolerance by detoxifying reactive aldehydes derived from lipid peroxidation. Medium- to long-chain saturated aldehydes are preferred substrates, while the short-chain aldehyde propanal is a weak substrate. Can use both NAD(+) and NADP(+), but the coenzyme preference is substrate dependent (550 aa ...
No significant hydrocarbons peaks in samples extracted from cells expressing only ADC. The blue GCMS chromatagram trace on the bottom show no significant peaks are found on the ADC only control within the time range that we expect alkane signal based upon GC-MS runs on chemical standards (8-10.5 minutes). This is expected, as E. coli does not normally produce any long chain length aldehyde substrates. C16 alcohols are observed when AAR is expressed on its own. The red GCMS chromatagram trace in the middle has a significant peak at 10.2 minutes corresponding to the C16 alcohol (as confirmed by comparison of the peaks MS spectra to a reference library) is observed when AAR is expressed in MG1655 E. coli. The production of C16 alcohols in cells expressing AAR (but not in cells expressing only ADC) is consistent with AAR reducing even chain length Acyl-ACPs into even chain length fatty aldehydes, which are further reduced by aldehyde dehydrogenases to the alcohol. The PetroBrick Works! C13, C15, ...
Spectral data for 41 aldehydes have been recorded from 1/700 cm to 1/300 cm and analyzed for spectra-structure correlations. The information so attained can be applied in identifying aliphatic and aromatic aldehydes. Specific correlations were determined for aliphatic aldehydes with or without alpha-branching. Tentative correlations were observed for alphaolefinic, alicyclic, and aromatic aldehydes. (Author)
Background: Cancer stem cells have tumor-initiation and tumor-maintenance capabilities. Stem-like cells are present in colorectal adenomas, but their relationship to adenoma pathology and patient characteristics, including metachronous development of an additional adenoma (recurrence), have not been studied extensively. We evaluated the expression of aldehyde dehydrogenase isoform 1A1 (ALDH1A1), a putative stem cell marker, in baseline adenomas from the placebo arm of chemoprevention trial participants with colonoscopic follow-up. Methods: An exploratory set of 20 baseline adenomas was analyzed by ALDH1A1 immunohistochemistry with morphometry, and a replication set of 89 adenomas from 76 high-risk participants was evaluated by computerized image analysis. Results: ALDH1A1 labeling indices (ALIs) were similar across patient characteristics and in advanced and non-advanced adenomas. There was a trend toward higher ALIs in adenomas occurring in the right than left colon (p=0.09). ALIs of ...
The major metabolic pathways involved in synthesis and disposition of carbonyl and hydroxyl group containing compounds are presented, and structural and functional characteristics of the enzyme families involved are discussed. Alcohol and aldehyde dehydrogenases (ADH, ALDH) participate in oxidative pathways, whereas reductive routes are accomplished by members of the aldo-keto reductase (AKR), short-chain dehydrogenases/reductases (SDR) and quinone reductase (QR) superfamilies. A wealth of biochemical, genetic and structural data now establishes these families to constitute important phase I enzymes.
Aldehyde Dehydrogenase 1 family, member A3 (ALDH1A3), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein stucture analysis and in cell biology research applications.
Alcohol-sensitive individuals lack aldehyde dehydrogenase (ALDH) and accumulate acetaldehyde which causes the unpleasant symptoms, and is also mutagenic and carcinogenic. Our discovery: sulforaphane (e.g. from broccoli sprouts) and similar inducers of cytoprotective enzymes boosts ALDH and accelerates the disposal of acetaldehyde.. ...
3-hydroxyacyl-CoA dehydrogenase activity. • RNA binding. • acetyl-CoA C-acyltransferase activity. • long-chain-enoyl-CoA ... long-chain-3-hydroxyacyl-CoA dehydrogenase activity. • GO:0001948 protein binding. • catalytic activity. • transferase activity ... HADHB, ECHB, MSTP029, MTPB, TP-BETA, hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional ... "Entrez Gene: hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional ...
Hydroxyacyl-Coenzyme A dehydrogenase. To acetyl-CoA. *Malonyl-CoA decarboxylase. Aldehydes. *Long-chain-aldehyde dehydrogenase ...
acyl-CoA dehydrogenase activity. • identical protein binding. • oxidoreductase activity. • medium-chain-acyl-CoA dehydrogenase ... ACADM, acyl-CoA dehydrogenase, C-4 to C-12 straight chain, ACAD1, MCAD, MCADH, acyl-CoA dehydrogenase medium chain. ... "Mitochondrially encoded NADH dehydrogenase 1". Cardiac Organellar Protein Atlas Knowledgebase (COPaKB).. *^ Matsubara Y, Kraus ... ACADM (acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain) is a gene that provides instructions for making an enzyme ...
2cg5: STRUCTURE OF AMINOADIPATE-SEMIALDEHYDE DEHYDROGENASE-PHOSPHOPANTETHEINYL TRANSFERASE IN COMPLEX WITH CYTOSOLIC ACYL ... "The SDR (short-chain dehydrogenase/reductase and related enzymes) nomenclature initiative". Chem Biol Interact. 178 (1-3): 94- ...
"aldehyde dehydrogenase - Homo sapiens". BRENDA. Technische Universität Braunschweig. January 2015. Retrieved 13 April 2015. ... and then aldehyde dehydrogenase (ALDH), which converts it to phenylacetic acid. This means that for significant concentrations ... product which is produced by monoamine oxidase and then further metabolized into β-phenylacetic acid by aldehyde dehydrogenase ... urinary metabolite of phenethylamine and is produced via monoamine oxidase metabolism and subsequent aldehyde dehydrogenase ...
The aldehyde dehydrogenases are a family of isozymes that may play a major role in the detoxification of aldehydes generated by ... Aldehyde dehydrogenase 3 family, member B1 also known as ALDH3B1 is an enzyme that in humans is encoded by the ALDH3B1 gene. ... Hsu LC, Chang WC, Yoshida A (Dec 1994). "Cloning of a cDNA encoding human ALDH7, a new member of the aldehyde dehydrogenase ... Hsu LC, Chang WC, Yoshida A (Dec 1994). "Cloning of a cDNA encoding human ALDH7, a new member of the aldehyde dehydrogenase ...
Aldehyde dehydrogenase 1 family, member A3, also known as ALDH1A3 or retinaldehyde dehydrogenase 3 (RALDH3), is an enzyme that ... Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol ... Rexer BN, Zheng WL, Ong DE (2001). "Retinoic acid biosynthesis by normal human breast epithelium is via aldehyde dehydrogenase ... Yoshida A, Rzhetsky A, Hsu LC, Chang C (1998). "Human aldehyde dehydrogenase gene family". Eur. J. Biochem. 251 (3): 549-57. ...
doi:10.1016/0006-2952(93)90285-5. Dyck, Lillian E. (1993). "Absence of the atypical mitochondrial aldehyde dehydrogenase (ALDH2 ... doi:10.1016/0024-3205(93)90442-6. Dyck, Lillian E. (1990). "Isoenzymes of aldehyde dehydrogenase in human lymphocytes". ...
... is the first known aldehyde dehydrogenase activator. Ma X, Luo Q, Zhu H, Liu X, Dong Z, Zhang K, et al. (May 2018). " ... Chen CH, Budas GR, Churchill EN, Disatnik MH, Hurley TD, Mochly-Rosen D (September 2008). "Activation of aldehyde dehydrogenase ... Chen CH, Ferreira JC, Gross ER, Mochly-Rosen D (January 2014). "Targeting aldehyde dehydrogenase 2: new therapeutic ... "Aldehyde dehydrogenase 2 activation ameliorates CCl4 -induced chronic liver fibrosis in mice by up-regulating Nrf2/HO-1 ...
DOPAL is detoxified mainly by aldehyde dehydrogenase. "3,4-dihydroxyphenylacetaldehyde - Compound Summary". PubChem Compound. ... Li, W., Spaziano, V.T., Burke, WJ., "Synthesis of a biochemically important aldehyde - 3,4-dihydroxyphenylacetaldehyde", Bio- ...
Gama-Castro S.; Núñez C.; Segura D.; Moreno S.; Guzmán J. & Espín G. (2001). "Azotobacter vinelandii Aldehyde Dehydrogenase ... it is also affected by aldehyde dehydrogenase[13] and the response regulator AlgR.[14] ...
NADH-alcohol dehydrogenase; NADH-aldehyde dehydrogenase; primary alcohol dehydrogenase; yeast alcohol dehydrogenase, NAD+ ... alcohol dehydrogenase (NAD); aliphatic alcohol dehydrogenase; ethanol dehydrogenase; NAD-dependent alcohol dehydrogenase; NAD- ... Oksidoreduktase alkohol:NAD+ (bahasa Inggris: aldehyde reductase; ...
2006;42(1):8-16 Crabb DW (2004). "Overview of the role of alcohol dehydrogenase and aldehyde dehydrogenase and their variants ... Alcohol metabolism depends on the enzymes alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH). Genetic variants of ... Edenberg HJ, McClintick JN (2018). "Alcohol Dehydrogenases, Aldehyde Dehydrogenases, and Alcohol Use Disorders: A Critical ... Koppaka V (2012). "Aldehyde Dehydrogenase Inhibitors: a Comprehensive Review of the Pharmacology, Mechanism of Action, ...
Human ALDH1A1 aldehyde dehydrogenase is capable of oxidizing malondialdehyde. Malondialdehyde and other thiobarbituric reactive ... This compound is a reactive aldehyde and is one of the many reactive electrophile species that cause toxic stress in cells and ... The production of this aldehyde is used as a biomarker to measure the level of oxidative stress in an organism. Malondialdehyde ...
"Entrez Gene: ALDH18A1 aldehyde dehydrogenase 18 family, member A1". Liu, Li-Kai; Becker, Donald F.; Tanner, John J. (2017-10-15 ... In many prokaryotes, proline dehydrogenase and P5C dehydrogenase form a bifunctional enzyme that prevents the release of P5C ... the enzyme proline dehydrogenase produces P5C from proline, and the enzyme 1-pyrroline-5-carboxylate dehydrogenase converts GSA ...
Betaine aldehyde is further oxidised in the mitochondria in mice to betaine by the enzyme betaine aldehyde dehydrogenase (EC ... In humans betaine aldehyde activity is performed by a nonspecific cystosolic aldehyde dehydrogenase enzyme (EC 1.2.1.3) TMG is ... "BRENDA - Information on EC 1.2.1.8 - betaine-aldehyde dehydrogenase". Brenda-enzymes.org. Retrieved 2016-07-07. Chern, M. K.; ... Pietruszko, R. (1999). "Evidence for mitochondrial localization of betaine aldehyde dehydrogenase in rat liver: purification, ...
"Aldehyde dehydrogenases in cellular responses to oxidative/electrophilic stress". Free Radical Biology and Medicine. 56: 89-101 ...
Other studies have suggested that reduced activity by the enzyme aldehyde dehydrogenase may be responsible for a build-up of ... March 1993). "Analysis of corneal aldehyde dehydrogenase patterns in pathologic corneas". Cornea. 12 (2): 146-54. doi:10.1097/ ...
"Entrez Gene: ALDH1L1 aldehyde dehydrogenase 1 family, member L1". Human ALDH1L1 genome location and ALDH1L1 gene details page ... The encoded protein belongs to the aldehyde dehydrogenase family and is responsible for formate oxidation in vivo. Deficiencies ... 10-formyltetrahydrofolate dehydrogenase is an enzyme that in humans is encoded by the ALDH1L1 gene. The protein encoded by this ... Krupenko SA, Oleinik NV (2002). "10-formyltetrahydrofolate dehydrogenase, one of the major folate enzymes, is down-regulated in ...
Aldehyde dehydrogenase family 3 member B2 is an enzyme that in humans is encoded by the ALDH3B2 gene. This gene encodes a ... "Entrez Gene: ALDH3B2 aldehyde dehydrogenase 3 family, member B2". Human ALDH3B2 genome location and ALDH3B2 gene details page ... Hsu LC, Chang WC (Dec 1996). "Sequencing and expression of the human ALDH8 encoding a new member of the aldehyde dehydrogenase ... member of the aldehyde dehydrogenase family, a group of isozymes that may play a major role in the detoxification of aldehydes ...
Aldehyde dehydrogenase 1 family, member A2, also known as ALDH1A2 or retinaldehyde dehydrogenase 2 (RALDH2), is an enzyme that ... "Entrez Gene: ALDH1A2 aldehyde dehydrogenase 1 family, member A2". CS1 maint: discouraged parameter (link) Duester G (September ... This protein belongs to the aldehyde dehydrogenase family of proteins. The product of this gene is an enzyme that catalyzes the ... Wang X, Penzes P, Napoli JL (1996). "Cloning of a cDNA encoding an aldehyde dehydrogenase and its expression in Escherichia ...
Sládek NE (August 1999). "Aldehyde dehydrogenase-mediated cellular relative insensitivity to the oxazaphosphorines". Current ...
"Drosophila melanogaster alcohol dehydrogenase: mechanism of aldehyde oxidation and dismutation". The Biochemical Journal. 329 ... Adh: Alcohol dehydrogenase- Drosophila melanogaster can express the alcohol dehydrogenase (ADH) mutation, thereby preventing ... "Alpha-ketoglutarate reduces ethanol toxicity in Drosophila melanogaster by enhancing alcohol dehydrogenase activity and ... the breakdown of toxic levels of alcohols into aldehydes and ketones.[40] While ethanol produced by decaying fruit is a natural ...
These agents all exert their effect by being converted to nitric oxide in the body by mitochondrial aldehyde dehydrogenase ( ... the enzyme for this conversion was not discovered to be mitochondrial aldehyde dehydrogenase (ALDH2) until 2002.[4] ... "An essential role for mitochondrial aldehyde dehydrogenase in nitroglycerin bioactivation". Proceedings of the National Academy ...
... is then oxidized to lactic acid by aldehyde dehydrogenase. Lactaldehyde is a three-carbon atom species with a ... Methylglyoxal is converted to D-lactaldehyde by glycerol dehydrogenase (gldA). ... carbonyl group on the first carbon atom (making it an aldehyde), and a hydroxy group on the second carbon atom, making it a ...
This protein belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the ... Aldehyde dehydrogenase 1 family, member A1, also known as ALDH1A1 or retinaldehyde dehydrogenase 1 (RALDH1), is an enzyme that ... Ma S, Chan KW, Lee TK, Tang KH, Wo JY, Zheng BJ, Guan XY (July 2008). "Aldehyde dehydrogenase discriminates the CD133 liver ... Hsu LC, Tani K, Fujiyoshi T, Kurachi K, Yoshida A (June 1985). "Cloning of cDNAs for human aldehyde dehydrogenases 1 and 2". ...
Aldehyde dehydrogenase 8 family, member A1 also known as ALDH8A1 is an enzyme that in humans is encoded by the ALDH8A1 gene. ... This protein belongs to the aldehyde dehydrogenase family of enzymes. It was originally thought to play a role in a pathway of ... Davis I, Yang Y, Wherritt D, Liu A (June 2018). "Reassignment of the human aldehyde dehydrogenase ALDH8A1 (ALDH12) to the ... Lin M, Napoli JL (December 2000). "cDNA cloning and expression of a human aldehyde dehydrogenase (ALDH) active with 9-cis- ...
Algar EM, Abedinia M, VandeBerg JL, Holmes RS (1991). "Purification and properties of baboon corneal aldehyde dehydrogenase: ... King G, Holmes RS (Sep 1993). "Human corneal aldehyde dehydrogenase: purification, kinetic characterisation and phenotypic ... PDB: 1K87​; Lee YH, Nadaraia S, Gu D, Becker DF, Tanner JJ (Feb 2003). "Structure of the proline dehydrogenase domain of the ... In the case of the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH), in addition to the large number of ...
... alcohol dehydrogenase converts 1-butanol to butyraldehyde; this is then converted to butyric acid by aldehyde dehydrogenase. ... possibly because of its slower transformation by alcohol dehydrogenase. Liquid 1-butanol, as is common with most organic ...
... detoxication of the lipid peroxide-derived reactive aldehydes". Plant Cell Physiol. 43 (12): 1445-55. doi:10.1093/pcp/pcf187. ... Glutaryl-CoA dehydrogenase. *Isovaleryl coenzyme A dehydrogenase. *3-oxo-5beta-steroid 4-dehydrogenase ...
The oxidases xanthine oxidase, aldehyde oxidase, and sulfite oxidase[31] Legumes, whole grains, nuts[25] molybdenum deficiency ... Pervasive and required for several enzymes such as carboxypeptidase, liver alcohol dehydrogenase, and carbonic anhydrase ...
pyruvate dehydrogenase (acetyl-transferring) activity. • oxidoreductase activity, acting on the aldehyde or oxo group of donors ... PDHA2, PDHAL, Pyruvate dehydrogenase (lipoamide) alpha 2, pyruvate dehydrogenase alpha 2, pyruvate dehydrogenase E1 alpha 2 ... Pyruvate dehydrogenase (lipoamide) alpha 2, also known as pyruvate dehydrogenase E1 component subunit alpha, testis-specific ... Mutations in the PDHA2 gene have been known to cause one form of pyruvate dehydrogenase deficiency. Pyruvate dehydrogenase ...
l-galactonolactone dehydrogenase.[131] to produce ascorbic acid.[122] l-Ascorbic acid has a negative feedback on l-galactose ... The aldehyde group of this compound is reduced to a primary alcohol using the enzyme glucuronate reductase and the cofactor ... l-galactose dehydrogenase, whereby the lactone ring opens and forms again but with between the carbonyl on C1 and hydroxyl ... UDP-glucose 6-dehydrogenase uses the co-factor NAD+ as the electron acceptor. The transferase UDP-glucuronate pyrophosphorylase ...
The aldehyde group of this compound is reduced to a primary alcohol using the enzyme glucuronate reductase and the cofactor ... UDP-glucose 6-dehydrogenase uses the co-factor NAD+ as the electron acceptor. The transferase UDP-glucuronate pyrophosphorylase ... Leferink, N. G.; van den Berg, W. A.; van Berkel, W. J. (2008). "L-Galactono-γ-lactone Dehydrogenase from Arabidopsis thaliana ... L-Galactose reacts with the enzyme L-galactose dehydrogenase, whereby the lactone ring opens and forms again but with between ...
Furthermore, alcohol dehydrogenase is present in the stomach lining. After absorption, the alcohol passes to the liver through ... but reach lower aldehyde concentrations and have milder hangovers. Rate of detoxification of alcohol can also be slowed by ... Alcohol is metabolized mainly by the group of six enzymes collectively called alcohol dehydrogenase. These convert the ethanol ... Such persons have impaired acetaldehyde dehydrogenase, which causes acetaldehyde levels to peak higher, producing more severe ...
pyruvate dehydrogenase (acetyl-transferring) activity. • oxidoreductase activity, acting on the aldehyde or oxo group of donors ... pyruvate dehydrogenase alpha 1, pyruvate dehydrogenase E1 alpha 1 subunit, pyruvate dehydrogenase E1 subunit alpha 1. ... pyruvate dehydrogenase complex. • mitochondrial matrix. • هسته یاخته. • هستک. • میتوکندری. فرایند زیستی. • mitochondrial acetyl ... pyruvate dehydrogenase (NAD+) activity. • GO:0001948 پیوند پروتئینی. ترکیبات سلولی. • intracellular membrane-bounded organelle ...
alcohol dehydrogenase (NADP+) activity. • retinal dehydrogenase activity. • allyl-alcohol dehydrogenase activity. • NADP- ... cDNAs and deduced amino acid sequences of human aldehyde and aldose reductases". The Journal of Biological Chemistry. 264 (16 ... AR belongs to the aldehyde-keto reductase superfamily, with a widely expression in human organs including the kidney, lens, ... It also participates in glucose metabolism and osmoregulation and plays a protective role against toxic aldehydes derived from ...
catalyzed by retinol dehydrogenases (RDHs)[9] and alcohol dehydrogenases (ADHs).[10] Retinol is called vitamin A alcohol, or ... It was originally called retinene,[2] and renamed[3] after it was discovered to be vitamin A aldehyde.[4][5] Retinal is one of ... catalyzed by retinal dehydrogenases[11] also known as retinaldehyde dehydrogenases (RALDHs)[10] as well as retinal oxidases.[12 ... BALL, S; GOODWIN, TW; MORTON, RA (1946). "Retinene1-vitamin A aldehyde". The Biochemical Journal. 40 (5-6): lix. PMID 20341217. ...
"aldehyde dehydrogenase - Homo sapiens". BRENDA. Technische Universität Braunschweig. January 2015. Retrieved 13 April 2015.. ... and then aldehyde dehydrogenase (ALDH), which convert it to phenylacetic acid.[5] This means that for significant ... product which is produced by monoamine oxidase and then further metabolized into β-phenylacetic acid by aldehyde dehydrogenase. ... urinary metabolite of phenethylamine and is produced via monoamine oxidase metabolism and subsequent aldehyde dehydrogenase ...
... class 3 aldehyde dehydrogenase, glucose-6-phosphate isomerase, and tissue kallikrein (function unknown)[5] ... Minor enzymes include salivary acid phosphatases A+B, N-acetylmuramoyl-L-alanine amidase, NAD(P)H dehydrogenase (quinone), ...
aldehyde dehydrogenase NAD(P)+ Ja 1.2.1.8 betaine-aldehyde dehydrogenase Ja 1.2.1.12 glyceraldehyde-3-phosphate dehydrogenase ( ... oxoglutarate dehydrogenase (succinyl-transferring) Ja 1.2.4.4 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl- ... L-iditol 2-dehydrogenase Ja 1.1.1.15 D-iditol + NAD+ ⇌. {\displaystyle \rightleftharpoons }. D-sorbose + NADH + H+ D-iditol 2- ... Aldehyde of keton + NADH + H+ Alcoholdehydrogenase (NAD+) Ja 1.1.1.2 Alcohol + NADP+ ⇌. {\displaystyle \rightleftharpoons }. ...
... of aldehyde dehydrogenase deficiency or chronic fatigue syndrome. Severe pain after drinking alcohol may indicate a more ... Alcohol intolerance is due to a genetic polymorphism of the enzyme alcohol dehydrogenase, the enzyme that metabolises ingested ... together with other substances to the accumulation of harmful Acetaldehyde by inhibiting the enzyme acetaldehyde dehydrogenase ...
retinol dehydrogenase activity. • allyl-alcohol dehydrogenase activity. Cydrannau o'r gell. • cytosol. • extracellular exosome ... cellular aldehyde metabolic process. • aldehyde catabolic process. • D-glucuronate catabolic process. • L-ascorbic acid ... "The role of aldehyde reductase AKR1A1 in the metabolism of γ-hydroxybutyrate in 1321N1 human astrocytoma cells. ". Chem Biol ... "Structures of human and porcine aldehyde reductase: an enzyme implicated in diabetic complications. ". Acta Crystallogr D Biol ...
In bacteria, carbon monoxide is produced via the reduction of carbon dioxide by the enzyme carbon monoxide dehydrogenase, an Fe ... Carbon monoxide is, along with aldehydes, part of the series of cycles of chemical reactions that form photochemical smog. It ... Large quantities of aldehydes are produced by the hydroformylation reaction of alkenes, carbon monoxide, and H2. ...
Genetics: alleles associated with aldehyde dehydrogenase (ALDH) and flushing phenotypes (alcohol flush reaction) in Asians are ... "Alcohol metabolism in Asian-American men with genetic polymorphisms of aldehyde dehydrogenase". Annals of Internal Medicine. ... "The mutation in the mitochondrial aldehyde dehydrogenase (ALDH2) gene responsible for alcohol-induced flushing increases ... For example, some people (predominantly East Asians) have a mutation in their alcohol dehydrogenase gene that makes this enzyme ...
Most of the aldophosphamide is then oxidised by the enzyme aldehyde dehydrogenase (ALDH) to make carboxycyclophosphamide. A ... Kohn FR, Sladek NE (1985). "Aldehyde dehydrogenase activity as the basis for the relative insensitivity of murine pluripotent ...
Enzymes that transfer aldehyde or ketone groups and included in EC 2.2. This category consists of various transketolases and ... Herbst EA, MacPherson RE, LeBlanc PJ, Roy BD, Jeoung NH, Harris RA, Peters SJ (Jan 2014). "Pyruvate dehydrogenase kinase-4 ... Transaldolase, the namesake of aldehyde transferases, is an important part of the pentose phosphate pathway. The reaction it ... and the regulation of pyruvate dehydrogenase (PDH), which converts pyruvate to acetyl CoA. Transferases are also utilized ...
Mechanism of oxidation of primary alcohols to carboxylic acids via aldehydes and aldehyde hydrates ... which has a higher affinity for liver alcohol dehydrogenase. In this way methanol will be excreted intact in urine.[21][22][23] ... If a higher priority group is present (such as an aldehyde, ketone, or carboxylic acid), then the prefix hydroxy-is used,[14] e ... Aldehydes or ketones are reduced with sodium borohydride or lithium aluminium hydride (after an acidic workup). Another ...
The aldehyde groups of the triose sugars are oxidised, and inorganic phosphate is added to them, forming 1,3- ... The G6P is then converted to 6-phosphogluconolactone in the presence of enzyme glucose-6-phosphate dehydrogenase( an oxido- ... From here, GA is oxidized via GA dehydrogenase into glycerate. The glycerate is phosphorylated by glycerate kinase into 2PG. ... The G3P is converted to 1,3-bisphosphoglycerate int the presence of enzyme glyceraldehyde-3-phosphate dehydrogenase (an oxido- ...
Hydroxyacyl-Coenzyme A dehydrogenase. To acetyl-CoA. *Malonyl-CoA decarboxylase. Aldehydes. *Long-chain-aldehyde dehydrogenase ...
Crabb D, Xiao Q (Jun 1998). "Studies on the enzymology of aldehyde dehydrogenase-2 in genetically modified HeLa cells". ... Acetaldehyde dehydrogenase. (acetylating). Crystallographic structure of the acetaldehyde dehydrogenase from Pseudomonas sp.[1] ... Acetaldehyde dehydrogenases (EC 1.2.1.10) are dehydrogenase enzymes which catalyze the conversion of acetaldehyde into acetic ... Hempel J, von Bahr-Lindström H, Jörnvall H (May 1984). "Aldehyde dehydrogenase from human liver. Primary structure of the ...
Protein yìí wà ní ẹbí aldehyde dehydrogenase ti àwọn protein. Gene yìí máa ń kóòdù fún succinic semialdehyde dehydrogenase tí ó ... "Entrez Gene: ALDH5A1 aldehyde dehydrogenase 5 family, member A1 (succinate-semialdehyde dehydrogenase)".. ... "A functional polymorphism in the succinate-semialdehyde dehydrogenase (aldehyde dehydrogenase 5 family, member A1) gene is ... Aldehyde dehydrogenase 5 family, member A1. Lát'ọwọ́ Wikipedia, ìwé ìmọ̀ ọ̀fẹ́ ...
2007.urtean, "methanol dehydrogenase" nobela batekanlantanoideak entzimen kofaktore bezala aurkituak izan ziren " ... New Anhydrous Lithium Lanthanide Binaphtholates and Their Use in Enantioselective Alkyl Addition to Aldehydes»] Organometallics ...
GABA can also be synthesized from putrescine[48][49] by diamine oxidase and aldehyde dehydrogenase.[48] ... Succinic semialdehyde is then oxidized into succinic acid by succinic semialdehyde dehydrogenase and as such enters the citric ...
... releasing HCl and ammonia to form an aldehyde.[35] The aldehyde group can further react with another amino group to form a ... Venkobachar et al.[46] found that succinic dehydrogenase was inhibited in vitro by HOCl, which led to the investigation of the ... Succinate dehydrogenase was also inhibited by HOCl, stopping the flow of electrons to oxygen. Later studies[38] revealed that ... Rakita, RM; Michel, BR; Rosen, H (1990). "Differential inactivation of Escherichia coli membrane dehydrogenases by a ...
Aldehyde/oxo oxidoreductases (EC 1.2). 1.2.1: NAD or NADP. *Aldehyde dehydrogenase *Acetaldehyde dehydrogenase ... alpha-ketoacid dehydrogenase activity. Cellular component. • mitochondrial alpha-ketoglutarate dehydrogenase complex. • ... 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial is an enzyme that in humans is encoded by the BCKDHA gene.[5] ... oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor. • protein binding. • metal ion ...
... aldehyde dehydrogenase, and converted to acetate, most of which enters the bloodstream and is ultimately oxidized to carbon ... Other articles where Aldehyde dehydrogenase is discussed: alcohol consumption: Processing in the liver: …acted upon by another ... acted upon by another enzyme, aldehyde dehydrogenase, and converted to acetate, most of which enters the bloodstream and is ...
... this breaks down various aldehydes into the corresponding carboxylic acids. Its most notable substrate is, of course ethanal ... Or ALDH, this breaks down various aldehydes into the corresponding carboxylic acids. Its most notable substrate is, of course ...
Aldehyde dehydrogenases (EC 1.2.1.3) are a group of enzymes that catalyse the oxidation of aldehydes. They convert aldehydes (R ... Aldehyde dehydrogenase is a polymorphic enzyme responsible for the oxidation of aldehydes to carboxylic acids, which leave the ... The active site of a human mitochondrial aldehyde dehydrogenase 2. Cys302 and Glu268 interact with the aldehyde substrate. The ... Marchitti SA, Brocker C, Stagos D, Vasiliou V (June 2008). "Non-P450 aldehyde oxidizing enzymes: the aldehyde dehydrogenase ...
Aldehyde dehydrogenaseImported. ,p>Information which has been imported from another database using automatic procedures.,/p> ,p ... tr,Q7M053,Q7M053_RAT Aldehyde dehydrogenase OS=Rattus norvegicus OX=10116 PE=1 SV=1 BSGZPTAVMYILFHKR Align. Format. Add to ...
In enzymology, a betaine-aldehyde dehydrogenase (EC 1.2.1.8) is an enzyme that catalyzes the chemical reaction betaine aldehyde ... Other names in common use include betaine aldehyde oxidase, BADH, betaine aldehyde dehydrogenase, and BetB. This enzyme ... ROTHSCHILD HA, BARRON ES (1954). "The oxidation of betaine aldehyde by betaine aldehyde dehydrogenase". J. Biol. Chem. 209 (2 ... Eklund H; El-Ahmad, M; Ramaswamy, S; Hjelmqvist, L; Jörnvall, H; Eklund, H (1998). "Structure of betaine aldehyde dehydrogenase ...
GO:0008802 betaine-aldehyde dehydrogenase activity GO:0016491 oxidoreductase activity GO:0016620 oxidoreductase activity, ... acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor GO:0046872 metal ion binding ...
Aldehyde oxidase/xanthine dehydrogenase, a/b hammerhead (IPR000674). Short name: Ald_Oxase/Xan_DH_a/b ... The aldehyde oxidase and xanthine dehydrogenase, a/b hammerhead domain is an evolutionary conserved protein domain [PMID: ... Aldehyde oxidase (EC:1.2.3.1) catalyses the conversion of an aldehyde in the presence of oxygen and water to an acid and ... Xanthine dehydrogenase (EC:1.1.1.204) catalyses the hydrogenation of xanthine to urate, and also requires FAD, molybdenum and ...
NAD-dependent aldehyde dehydrogenase activity, NAD-linked aldehyde dehydrogenase activity, aldehyde dehydrogenase (NAD+), ... aldehyde dehydrogenase (NAD+), aldehyde:NAD+ oxidoreductase activity, CoA-independent aldehyde dehydrogenase activity, m- ... propionaldehyde dehydrogenase activity, CoA-independent aldehyde dehydrogenase activity, NAD-aldehyde dehydrogenase activity, ... NAD-aldehyde dehydrogenase activity, NAD-dependent 4-hydroxynonenal dehydrogenase activity, NAD-dependent aldehyde ...
aldehyde dehydrogenase X, mitochondrial. Names. ALDH class 2. acetaldehyde dehydrogenase 5. aldehyde dehydrogenase 5. ... ALDH1B1 aldehyde dehydrogenase 1 family member B1 [Homo sapiens] ALDH1B1 aldehyde dehydrogenase 1 family member B1 [Homo ... This protein belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the ... aldehyde dehydrogenase 1 family member B1provided by HGNC. Primary source. HGNC:HGNC:407 See related. Ensembl:ENSG00000137124 ...
Aldehyde dehydrogenase activity promotes survival of human muscle precursor cells.. Jean E1, Laoudj-Chenivesse D, Notarnicola C ... Aldehyde dehydrogenases (ALDH) are a family of enzymes that efficiently detoxify aldehydic products generated by reactive ...
Keywords: Arabidopsis thaliana; Craterostigma plantagineum; abscisic acid; aldehyde dehydrogenase; dehydration; enzyme activity ... Phylogenetically, the Cp- and Ath-ALDH3 and -ALDH4 proteins are closely related to aldehyde dehydrogenases from bacteria and ... Novel ABA- and dehydration-inducible aldehyde dehydrogenase genes isolated from the resurrection plant Craterostigma ... a gene was isolated with homology to class 3 variable substrate aldehyde dehydrogenases (ALDH). The C. plantagineum gene Cp- ...
tr,A0A658YN00,A0A658YN00_CAMCO Aldehyde dehydrogenase OS=Campylobacter coli OX=195 GN=aldA PE=4 SV=1 ... Aldehyde dehydrogenase. Lacticaseibacillus paracasei subsp. tolerans. 488. Aldehyde dehydrogenase. Lactobacillus sp. HMSC25A02 ...
SEARCH RESULTS for: Acetyl Aldehyde Dehydrogenase Inhibitors [Drug Class] (10 results) * Share : JavaScript needed for Sharing ...
Aldehyde dehydrogenases (ALDHs) are highly expressed in the chemotherapy- and radiotherapy-resistant cell subpopulations of ... Aldehyde Dehydrogenase Inhibitors for Cancer Therapeutics Trends Pharmacol Sci. 2019 Oct;40(10):774-789. doi: 10.1016/j.tips. ... Aldehyde dehydrogenases (ALDHs) are highly expressed in the chemotherapy- and radiotherapy-resistant cell subpopulations of ... Keywords: aldehyde dehydrogenase; cancer pharmacology; cancer resistance; cancer stem cells; cancer therapy; targeted ...
BETAINE ALDEHYDE DEHYDROGENASE. A, B, D, F, G, H. 490. Pseudomonas aeruginosa. Mutation(s): 0 EC: 1.2.1.8. ... BETAINE ALDEHYDE DEHYDROGENASE. C, E. 490. Pseudomonas aeruginosa. Mutation(s): 0 EC: 1.2.1.8. ... In the human pathogen Pseudomonas aeruginosa, the NAD(P)(+)-dependent betaine aldehyde dehydrogenase (PaBADH) may play the dual ... In the human pathogen Pseudomonas aeruginosa, the NAD(P)(+)-dependent betaine aldehyde dehydrogenase (PaBADH) may play the dual ...
2011) Aldehyde dehydrogenase: Its role as a cancer stem cell marker comes down to the specific isoform. Cell Cycle 10(9):1378- ... Aldehyde Dehydrogenase 1A3 Activity Is Markedly Elevated in Mes GSCs Compared with Mes Non-GSCs and PN GSCs.. Based on the ... 2009) Aldehyde dehydrogenase 1 is a marker for normal and malignant human colonic stem cells (SC) and tracks SC overpopulation ... 2011) Aldehyde dehydrogenase activity of breast cancer stem cells is primarily due to isoform ALDH1A3 and its expression is ...
We focus on the characterization of the aldehyde dehydrogenase-1 family of enzymes (ALDH1A1, ALDH1A2, ALDH1A3) that catalyze ... Aldehyde Dehydrogenase 1A1: Friend or Foe to Female Metabolism?. Jennifer M. Petrosino. ... Petrosino, J.M.; DiSilvestro, D.; Ziouzenkova, O. Aldehyde Dehydrogenase 1A1: Friend or Foe to Female Metabolism? Nutrients ... "Aldehyde Dehydrogenase 1A1: Friend or Foe to Female Metabolism?" Nutrients 6, no. 3: 950-973. ...
Oxidizes medium and long chain aldehydes into non-toxic fatty acids (PubMed:2831537). Preferentially oxidizes aromatic aldehyde ... An aldehyde + NADP+ + H2O = a carboxylate + NADPH. UniProt ... Aldehyde dehydrogenase, dimeric NADP-preferring - P11883 (AL3A1 ...
Inhibition of aldehyde dehydrogenase and retinoid signaling induces the expansion of human hematopoietic stem cells. John P. ... Aldehyde dehydrogenase (ALDH) is an enzyme that is expressed in the liver and is required for the conversion of retinol ( ... Inhibition of aldehyde dehydrogenase and retinoid signaling induces the expansion of human hematopoietic stem cells ... Inhibition of aldehyde dehydrogenase and retinoid signaling induces the expansion of human hematopoietic stem cells ...
Compare aldehyde dehydrogenase 1 family member L1 ELISA Kits from leading suppliers on Biocompare. View specifications, prices ... aldehyde dehydrogenase 1 family member L1 ELISA Kits. The ELISA (enzyme-linked immunosorbent assay) is a well-established ... Your search returned 21 aldehyde dehydrogenase 1 family member L1 ELISA ELISA Kit across 3 suppliers. ...
Compare Anti-aldehyde dehydrogenase 5 family member A1 Antibody Products from leading suppliers on Biocompare. View ... Anti-aldehyde dehydrogenase 5 family member A1 Antibody Products. Anti-Aldehyde dehydrogenase 5 family member A1 antibodies are ... Your search returned 192 aldehyde dehydrogenase 5 family member A1 Antibodies across 28 suppliers. ... The protein may also be known as SSADH, SSDH, succinate-semialdehyde dehydrogenase, mitochondrial, and NAD(+)-dependent ...
"Distribution of messenger RNAs for aldehyde dehydrogenase 1, aldehyde dehydrogenase 2, and aldehyde dehydrogenase 5 in human ... M.-K. Chern and R. Pietruszko, "Human aldehyde dehydrogenase E3 isozyme is a betaine aldehyde dehydrogenase," Biochemical and ... G.-S. Peng and S.-J. Yin, "Effect of the allelic variants of aldehyde dehydrogenase ALDH2∗2 and alcohol dehydrogenase ADH1B∗2 ... "Aldehyde dehydrogenase 2 (ALDH2) rescues myocardial ischaemia/reperfusion injury: role of autophagy paradox and toxic aldehyde ...
... encoding aldehyde dehydrogenase 7 in wheat, conferred significant drought tolerance to Arabidopsis , supported by molecular ... Aldehyde dehydrogenase (ALDH) is a family of enzymes catalyzing the irreversible conversion of aldehydes into acids to decrease ... Lindahl R (1992) Aldehyde dehydrogenases and their role in Carcinogenesis. Crit Rev Biochem Mol Biol 27:283-335PubMedCrossRef ... Yoshida A, Rzhetsky A, Hsu LC, Chang C (1998) Human aldehyde dehydrogenase gene family. Eur J Biochem 251:549-557PubMedCrossRef ...
AcrR and Rex Control Mannitol and Sorbitol Utilization through Their Cross-Regulation of Aldehyde-Alcohol Dehydrogenase (AdhE) ...
ALDH, aldehyde dehydrogenase; BALDH, betaine aldehyde dehydrogenase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GGSALDH, ... Kinetics and specificity of human liver aldehyde dehydrogenases toward aliphatic, aromatic, and fused polycyclic aldehydes. ... Hydrazone dehydrogenase, which belongs to the aldehyde dehydrogenase (ALDH) superfamily, attacked the C=N double bond of ... Group X Aldehyde Dehydrogenases of Pseudomonas aeruginosa PAO1 Degrade Hydrazones. Kosuke Taniyama, Hideomi Itoh, Atsushi ...
Vascular bioactivation of nitroglycerin is catalyzed by cytosolic aldehyde dehydrogenase-2. Download Prime PubMed App to iPhone ... AdenoviridaeAldehyde DehydrogenaseAldehyde Dehydrogenase, MitochondrialAnimalsAortaBiotransformationCell LineCytosolDNAHumans ... Bioactivation of pentaerythrityl tetranitrate by mitochondrial aldehyde dehydrogenase.. *Potent inhibition of aldehyde ... Changes in aldehyde dehydrogenase 2 expression in rat blood vessels during glyceryl trinitrate tolerance development and ...
IPR015590 Aldehyde dehydrogenase domain. IPR029510 Aldehyde dehydrogenase, glutamic acid active site. IPR016162 Aldehyde ...
IPR016163 Aldehyde dehydrogenase, C-terminal. IPR015590 Aldehyde dehydrogenase domain. IPR016162 Aldehyde dehydrogenase, N- ...
What is aldehyde dehydrogenase 3B1? Meaning of aldehyde dehydrogenase 3B1 medical term. What does aldehyde dehydrogenase 3B1 ... Looking for online definition of aldehyde dehydrogenase 3B1 in the Medical Dictionary? aldehyde dehydrogenase 3B1 explanation ... redirected from aldehyde dehydrogenase 3B1) ALDH3B1. A gene on chromosome 11q13 that encodes an aldehyde dehydrogenase that ... Aldehyde dehydrogenase 3B1 , definition of aldehyde dehydrogenase 3B1 by Medical dictionary https://medical-dictionary. ...
... we investigated the genotype of aldehyde dehydrogenase (ALDH) 2 and alcohol dehydrogenase 2 (ADH2) in DM-Mt3243. ... Mitochondrial Aldehyde Dehydrogenase in Diabetes Associated With Mitochondrial tRNALeu(UUR) Mutation at Position 3243. ... Mitochondrial Aldehyde Dehydrogenase in Diabetes Associated With Mitochondrial tRNALeu(UUR) Mutation at Position 3243 ... Mitochondrial Aldehyde Dehydrogenase in Diabetes Associated With Mitochondrial tRNALeu(UUR) Mutation at Position 3243 ...
  • Or ALDH, this breaks down various aldehyde s into the corresponding carboxylic acid s. (everything2.com)
  • Aldehyde dehydrogenases (ALDH) are a family of enzymes that efficiently detoxify aldehydic products generated by reactive oxygen species and might therefore participate in cell survival. (nih.gov)
  • In order to identify genes that are critical for the ABA-dependent stress response in the resurrection plant Craterostigma plantagineum , a gene was isolated with homology to class 3 variable substrate aldehyde dehydrogenases (ALDH). (ingentaconnect.com)
  • Aldehyde dehydrogenase (ALDH) is an enzyme that is expressed in the liver and is required for the conversion of retinol (vitamin A) to retinoic acids. (pnas.org)
  • 9 , 10 ) demonstrated that the intracellular enzyme, aldehyde dehydrogenase (ALDH), protected BM progenitors from the cytotoxic effects of cyclophosphamide by deactivation of its metabolite, 4-hydroxycyclophosphamide ( 9 , 10 ). (pnas.org)
  • Aldehyde dehydrogenase (ALDH) 2 is a mitochondrial enzyme that is known for its important role in oxidation and detoxification of ethanol metabolite acetaldehyde. (hindawi.com)
  • Aldehyde dehydrogenase (ALDH) 2 is a mitochondrial enzyme that catalyzes the oxidation of acetaldehyde, an intermediate of ethanol metabolism [ 1 ]. (hindawi.com)
  • Aldehyde dehydrogenase (ALDH) is a family of enzymes catalyzing the irreversible conversion of aldehydes into acids to decrease the damage caused by abiotic stresses. (springer.com)
  • Gene cloning indicated that the HDH is part of the group X aldehyde dehydrogenase (ALDH) family, which is distributed among bacteria, although the physiological roles of the ALDH family remain unknown. (asm.org)
  • OBJECTIVE To ascertain why alcohol is prone to manifest unpleasant effects in diabetes associated with mitochondrial tRNA Leu(UUR) mutation at position 3243 (DM-Mt3243), we investigated the genotype of aldehyde dehydrogenase (ALDH) 2 and alcohol dehydrogenase 2 (ADH2) in DM-Mt3243. (diabetesjournals.org)
  • In many types of tumors, especially breast tumors, aldehyde dehydrogenase (ALDH) activity has been used to identify cancer stem-like cells within the tumor. (dovepress.com)
  • ALDH activity measured fluorimetrically using a high concentration of aliphatic aldehyde as substrate was studied in human glioblastomas grafted in nude mice. (springer.com)
  • In view of previous works, these results suggest a relationship between alterations in ALDH iso-enzymes activities and cytosolic aldehyde concentrations with respect to normal or tumoral cell growth. (springer.com)
  • Important thing to know is that ALDH oxidizes aldehydes to carboxylic acids. (steadyhealth.com)
  • Experts are saying that Aldehyde dehydrogenase (ALDH) is functioning often in tandem with Alcohol dehydrogenase. (steadyhealth.com)
  • Using a rat model, we then showed that nociception tightly correlated with ALDH activity ( R 2 = 0.90) and that reduced nociception was associated with less early growth response protein 1 (EGR1) in the spinal cord and less reactive aldehyde accumulation at the insult site (including acetaldehyde and 4-hydroxynonenal). (sciencemag.org)
  • Background and Objectives Primitive human hematopoietic cells contain higher levels of aldehyde dehydrogenase (ALDH) activity than their terminally differentiating progeny but the particular stages at which ALDH levels change have not been well defined. (haematologica.org)
  • A complementary strategy for the functional identification and characterization of normal stem cells and their malignant counterparts involves the measurement of aldehyde dehydrogenase (ALDH) activity. (aacrjournals.org)
  • Aldehyde dehydrogenase (ALDH) 1A enzymes produce retinoic acid (RA), a transcription induction molecule. (ovid.com)
  • High aldehyde dehydrogenase (ALDH hi ) activity has been reported in normal and cancer stem cells. (frontiersin.org)
  • A widely used universal stem cell marker is aldehyde dehydrogenase (ALDH) ( 6 - 8 ). (frontiersin.org)
  • ALDH is a generic designation for a superfamily of NAD-dependent enzymes that catalyze the oxidation of aldehydes to acids. (frontiersin.org)
  • In the course of engineering these strains, a number of mutations have been discovered in their adhE genes, which encode both alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) enzymes. (asm.org)
  • Aldehyde dehydrogenase (ALDH) enzymes catalyze the NAD(P)+-dependent oxidation of a wide variety of endogenous and exogenous aldehydes to their corresponding acids. (ovid.com)
  • Here, we isolated by fluorescence activated cell sorting technique, two populations of Wharton's jelly (WJ)-MSCs based on their aldehyde dehydrogenase (ALDH) activity. (springer.com)
  • This reaction could take place by two different methods: via an acylating aldehyde dehydrogenase (ALDH) that could catalyze the reaction to yield pimeloyl-CoA in one step or via the oxidation of pimelic semialdehyde to pimelic acid by a nonacylating ALDH, including its subsequent activation by a CoA-ligase or a CoA-transferase. (pubmedcentralcanada.ca)
  • Before determining the IC50's for the Aldehyde Dehydrogenase (ALDH) inhibitor, we performed some initial experiments to establish appropriate assay conditions. (bioassaysys.com)
  • The IC50 determination required two steps: 1) 20 min pre-incubation of 45 µL ALDH (1.3 U/rxn) with 5 µL disulfiram in 100% DMSO, and 2) aldehyde dehydrogenase reaction with 900 µM final [acetaldehyde] for 30 min at 25°C. We titrated disulfiram from 0-50 µM and computed the ΔOD for each inhibitor concentration. (bioassaysys.com)
  • CSCs were isolated from SUM149 and MARY-X, an IBC cell line and primary xenograft, by virtue of increased aldehyde dehydrogenase (ALDH) activity as assessed by the ALDEFLUOR assay. (aacrjournals.org)
  • Lung cancer stem cells (CSC) with elevated aldehyde dehydrogenase (ALDH) activity are self-renewing, clonogenic, and tumorigenic. (aacrjournals.org)
  • This ELISA kit is based on the principle of double-antibody sandwich technique to detect Rat aldehyde dehydrogenase,ALDH ELISA Kit Be used only for research purposes, not be used for medical diagnosis. (ttnet.net)
  • BACKGROUND/OBJECTIVES: Heavy alcohol drinking is a risk factor of colorectal cancer (CRC), but little is known on the effect of polymorphisms in the alcohol-metabolizing enzymes, alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) on the alcohol-related risk of CRC in Caucasian populations. (lu.se)
  • article{01d9c5b7-1e40-4ebf-9258-6769f39c0b13, abstract = {BACKGROUND/OBJECTIVES: Heavy alcohol drinking is a risk factor of colorectal cancer (CRC), but little is known on the effect of polymorphisms in the alcohol-metabolizing enzymes, alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) on the alcohol-related risk of CRC in Caucasian populations. (lu.se)
  • Mitochondrial aldehyde dehydrogenase (ALDH2) is an isoenzyme of aldehyde dehydrogenases (ALDH), a group of enzymes that are responsible for clearance of aldehydes in the body. (eurekaselect.com)
  • Among the isoenzymes of ALDH, ALDH2 is believed to play a major role in clearance of toxic aldehydes. (eurekaselect.com)
  • After transplantation into β-glucuronidase (GUSB)-deficient NOD/SCID/mucopolysaccharidosis type VII mice, we characterized the distribution of lineage-depleted human umbilical cord blood-derived cells purified by selection using high aldehyde dehydrogenase (ALDH) activity with CD133 coexpression. (elsevier.com)
  • Aldehyde dehydrogenase (ALDH) activity is commonly used as a marker to identify cancer stem-like cells. (iupui.edu)
  • The second step of RA synthesis is controlled by members of the aldehyde dehydrogenase (ALDH) family also known as retinaldehyde dehydrogenase (RALDH) that further oxidize retinaldehyde to produce RA. (ozgene.com)
  • The purpose of this comprehensive meta-analysis was to assess the association of aldehyde dehydrogenase (ALDH) expression with overall survival (OS) and disease-free survival (DFS)/progression-free survival (PFS) in ovarian cancer patients. (biomedcentral.com)
  • Many ALDH isozymes are important in oxidizing reactive aldehydes derived from lipid peroxidation and thereby help maintain cellular homeostasis. (medchemexpress.com)
  • NCT-501 is a potent and selective theophylline-based inhibitor of aldehyde dehydrogenase 1A1 (ALDH1A1) , inhibits hALDH1A1 with IC 50 of 40 nM, typically shows better selectivity over other ALDH isozymes and other dehydrogenases (hALDH1B1, hALDH3A1, and hALDH2, IC 50 >57 μM). (medchemexpress.com)
  • CVT-10216 is a highly selective, reversible aldehyde dehydrogenase-2 (ALDH-2) inhibitor with an IC 50 of 29 nM. (medchemexpress.com)
  • ALDH1 and ALDH2 are the most important enzymes for aldehyde oxidation, and both are tetrameric enzymes composed of 54 kDa subunits. (wikipedia.org)
  • There is a mutant form of aldehyde dehydrogenase, termed ALDH2*2, wherein a lysine residue replaces a glutamate in the active site at position 487 of ALDH2. (wikipedia.org)
  • ALDH2 also metabolizes other reactive aldehydes such as 4-hydroxy-2-nonenal and acrolein. (hindawi.com)
  • In addition, ALDH2 is important for the detoxification of reactive aldehydes such as 4-HNE and acrolein [ 2 ]. (hindawi.com)
  • ALDH2 may be one of the essential mechanisms for the removal of these reactive aldehydes and protecting cells and organs from these toxic aldehydes. (hindawi.com)
  • According to general view, aldehyde dehydrogenase-2 (ALDH2) catalyzes the high-affinity pathway of vascular nitroglycerin (GTN) bioactivation in smooth muscle mitochondria. (unboundmedicine.com)
  • To be more precise - two acetaldehyde dehydrogenase isozymes, cytosolic (ALDH1) and mitochondrial (ALDH2), catalyze this reaction. (steadyhealth.com)
  • Exogenous aldehydes can cause pain in animal models, suggesting that aldehyde dehydrogenase-2 (ALDH2), which metabolizes many aldehydes, may regulate nociception. (sciencemag.org)
  • The atypical genotypes of low K m aldehyde dehydrogenase (ALDH2) have higher blood concentrations of free acetaldehyde after drinking alcohol. (aacrjournals.org)
  • To elucidate the mechanism underlying reduction of nitroglycerin (GTN) to nitric oxide (NO) by mitochondrial aldehyde dehydrogenase (ALDH2), we generated mutants of the enzyme lacking the cysteines adjacent to reactive Cys302 (C301S and C303S), the glutamate that participates as a general base in aldehyde oxidation (E268Q) or combinations of these residues. (aspetjournals.org)
  • Mitochondrial aldehyde dehydrogenase (ALDH2) displays some promise in the protection against cardiovascular diseases although its role in diabetes has not been elucidated. (biomedcentral.com)
  • Reactive aldehydes contribute to pain pathologies and cardiac injury, suggesting that aldehyde dehydrogenase (ALDH2), which detoxifies aldehydes, may regulate chronic pain related MI/R injury. (onlinejacc.org)
  • However, treatment of CCD-WT mice with ALDH2-selective activator (Alda-1) significantly reduced chronic neuropathic pain-induced SIRT1 carbonylative inactivation and decreased MI/R injury (minor infarct size, less apoptosis, and elevated cardiac function) likely as a result of prevented aldehyde overload and carbonyl stress. (onlinejacc.org)
  • ALDH2 activation blocked reactive aldehyde overproduction induced carbonyl stress and attenuated ischemic vulnerability in chronic pain individual. (onlinejacc.org)
  • Aldehyde dehydrogenase 2 (ALDH2) is an anti-apoptotic enzyme whose activity decline associates with myocardial injury. (eurekaselect.com)
  • Genetic variation in alcohol dehydrogenase (ADH1A, ADH1B, ADH1C, ADH7) and aldehyde dehydrogenase (ALDH2), alcohol consumption and gastric cancer risk in the European Prospective Investigation into Cancer and Nutrition (EPIC) cohort. (environment-health.ac.uk)
  • ADH7 and aldehyde dehydrogenase, ALDH2), alcohol intake and GC risk. (environment-health.ac.uk)
  • Acetaldehyde dehydrogenase 2 (ALDH2) is the key enzyme responsible for metabolism of the alcohol metabolite acetaldehyde in the liver. (ijbs.com)
  • 4-Hydroxynonenal is a substrate and an inhibitor of acetaldehyde dehydrogenase 2 (ALDH2) . (medchemexpress.com)
  • acted upon by another enzyme, aldehyde dehydrogenase, and converted to acetate, most of which enters the bloodstream and is ultimately oxidized to carbon dioxide and water. (britannica.com)
  • Aldehyde dehydrogenase is a polymorphic enzyme responsible for the oxidation of aldehydes to carboxylic acids, which leave the liver and are metabolized by the body's muscle and heart. (wikipedia.org)
  • The active site of the aldehyde dehydrogenase enzyme is largely conserved throughout the different classes of the enzyme and, although the number of amino acids present in a subunit can change, the overall function of the site changes little. (wikipedia.org)
  • A magnesium may be used to help the enzyme function, although the amount it helps the enzyme can vary between different classes of aldehydes. (wikipedia.org)
  • The overall reaction catalysed by the aldehyde dehydrogenases is: RCHO + NAD+ + H2O → RCOOH + NADH + H+ In this NAD(P)+-dependent reaction, the aldehyde enters the active site through a channel extending from the surface of the enzyme. (wikipedia.org)
  • In enzymology, a betaine-aldehyde dehydrogenase (EC 1.2.1.8) is an enzyme that catalyzes the chemical reaction betaine aldehyde + NAD+ + H2O ⇌ {\displaystyle \rightleftharpoons } betaine + NADH + 2 H+ The 3 substrates of this enzyme are betaine aldehyde, NAD+, and H2O, whereas its 3 products are betaine, NADH, and H+. (wikipedia.org)
  • This enzyme belongs to the family of oxidoreductases, specifically those acting on the aldehyde or oxo group of donor with NAD+ or NADP+ as acceptor. (wikipedia.org)
  • The systematic name of this enzyme class is betaine-aldehyde:NAD+ oxidoreductase. (wikipedia.org)
  • The enzyme can be converted from the dehydrogenase form to the oxidase form irreversibly by proteolysis or reversibly through oxidation of sulphydryl groups. (ebi.ac.uk)
  • Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. (nih.gov)
  • [1] [2] This gene is a member of the aldehyde dehydrogenase family and encodes a bifunctional ATP - and NADPH -dependent mitochondrial enzyme with both gamma-glutamyl kinase and gamma-glutamyl phosphate reductase activities. (wikidoc.org)
  • Succinate-semialdehyde dehydrogenase, mitochondrial is an enzyme that in humans is encoded by the ALDH5A1 gene . (wikidoc.org)
  • Low-autocrine RA generation by the cytosolic aldehyde dehydrogenase 1 (Aldh1a1, -a2, and -a3) enzyme family ( 14 ) stimulates adipogenesis via mechanisms dependent on transcription factors ZFP423 and PPARγ ( 15 ). (diabetesjournals.org)
  • The 3 substrates of this enzyme are betaine aldehyde , NAD + , and H 2 O , whereas its 3 products are betaine , NADH , and H + . (academic.ru)
  • Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. (creative-biogene.com)
  • Lack of the cysteines did not affect dehydrogenase activity but impeded GTN denitration, aggravated GTN-induced enzyme inactivation, and increased NO formation. (aspetjournals.org)
  • Gene expression studies indicate that the RF2C gene, which is strongly expressed in all organs, appears essential, suggesting that the crucial role of the enzyme would certainly be linked to the cell wall formation using aldehydes from phenylpropanoid pathway as substrates. (biochemj.org)
  • The kinetic properties of the mitochondrial enzyme, compared with those reported for the cytoplasmic aldehyde dehydrogenase from sheep liver, show significant differences, which may be important in the oxidation of aldehydes in vivo. (biochemj.org)
  • There is a deficiency of an enzyme called fatty aldehyde dehydrogenase 10 (FALDH10)in the Sjogren-Larsson syndrome. (drugster.info)
  • The topic of this work is of major importance since we show that invasion and metastasis in IBC are mediated by a cellular subcomponent with stem cell characteristics expressing the stem cell marker aldehyde dehydrogenase 1 (ALDH1). (aacrjournals.org)
  • To determine the differences in response following the use of aldehyde dehydrogenase 1 (ALDH1) and Raf kinase inhibitor protein (RKIP) as cervical cancer stem cell markers. (ajol.info)
  • In models of triple-negative breast cancer (TNBC), it has recently been shown that the epidermal growth factor receptor (EGFR) pathway is up-regulated in the aldehyde dehydrogenase 1 (ALDH1)-positive cancer stem cell fraction. (uzh.ch)
  • SR-T100 downregulated the expression of stem cell markers, including aldehyde dehydrogenase 1 (ALDH1), Notch1, and FoxM1, and reduced sphere formation in ovarian cancer cells. (jcancer.org)
  • The present study is to determine the expression of aldehyde dehydrogenase 1 (ALDH1) and ATPbinding cassette superfamily G member 2 (ABCG2) in patients with triple-negative breast cancer, and to understand the relationship of ALDH1 and ABCG2 with triple-negative breast cancer. (alliedacademies.org)
  • Disulfiram (Tetraethylthiuram disulfide) is a specific inhibitor of aldehyde-dehydrogenase (ALDH1) , used for the treatment of chronic alcoholism by producing an acute sensitivity to alcohol. (medchemexpress.com)
  • 4-Diethylaminobenzaldehyde is a reversible aldehyde dehydrogenases (ALDHs) inhibitor, with a K i of 4 nM for ALDH1. (medchemexpress.com)
  • The active site of a human mitochondrial aldehyde dehydrogenase 2. (wikipedia.org)
  • Kinetic studies were carried out on mitochondrial aldehyde dehydrogenase (EC 1.2.1.3) isolated from sheep liver. (biochemj.org)
  • Xiu-Ju Luo, Bin Liu, Qi-Lin Ma and Jun Peng, "Mitochondrial Aldehyde Dehydrogenase, A Potential Drug Target for Protection of Heart and Brain from Ischemia/Reperfusion Injury", Current Drug Targets (2014) 15: 948. (eurekaselect.com)
  • A gene on chromosome 11q13 that encodes an aldehyde dehydrogenase that plays a major role in detoxifying aldehydes generated by alcohol metabolism and lipid peroxidation. (thefreedictionary.com)
  • This gene encodes a mitochondrial NAD + -dependent succinic semialdehyde dehydrogenase . (wikidoc.org)
  • Gene expression profiling revealed that Aldehyde dehydrogenase 1 family, member A3 (ALDH1A3) is up regulated in OcMMP. (bl.uk)
  • The gene products are similar to nonacylating aldehyde dehydrogenases (ThnG) and to proteins representing a complete beta-oxidation pathway (ThnH to ThnP). (sigmaaldrich.com)
  • A gene on chromosome 17p11.2 that encodes an aldehyde dehydrogenase that plays a major role in detoxifying alcohol-derived acetaldehyde, metabolising corticosteroids, biogenic amines, neurotransmitters and in lipid peroxidation. (thefreedictionary.com)
  • The ALDH3A1 gene product forms a homodimer that preferentially oxidises aromatic and medium-chain (6 carbons or more) saturated and unsaturated aldehyde substrates. (thefreedictionary.com)
  • The qualitative change requires the derepression of a gene for an aldehyde dehydrogenase expressed in normal liver only after exposure to potentially harmful xenobiotics. (portlandpress.com)
  • Holmes, R. S., 1977, The genetics of a-hydroxyacid oxidase and alcohol dehydrogenase in the mouse: evidence for multiple gene loci and linkage between Hao-2 and Adh-3, Genetics 87:709. (springer.com)
  • Holmes, R. S., 1979a, Genetics and ontogeny of alcohol dehydrogenase isozymes in the mouse - evidence for a cis-acting regulator gene (Adh-3-t) controlling C 2 isozyme expression in reproductive tissues and close linkage of Adh-3 and Adh-3-t on chromosome 3, Biochem. (springer.com)
  • The protein encoded by this gene belongs to the aldehyde dehydrogenase family. (creative-biogene.com)
  • The ABA2 gene product belongs to the family of short-chain dehydrogenases/reductases, which are known to be NAD- or NADP-dependent oxidoreductases. (plantcell.org)
  • Hellström E, Tottmar O: Effects of aldehyde dehydrogenase inhibitors on enzymes involved in the metabolism of biogenic aldehydes in rat liver and brain. (springer.com)
  • Aldehyde dehydrogenases (ALDHs) constitute a superfamily of NAD(P) + -dependent enzymes that catalyse irreversible oxidation of aldehydes to the corresponding carboxylic acids. (biochemj.org)
  • Aldehyde Dehydrogenases (ALDHs) are a superfamily of NADP + -dependent enzymes that metabolize endogenous and exogenous aldehydes to corresponding carboxylic acids. (medchemexpress.com)
  • We focus on the characterization of the aldehyde dehydrogenase-1 family of enzymes (ALDH1A1, ALDH1A2, ALDH1A3) that catalyze conversion of retinaldehyde to retinoic acid. (mdpi.com)
  • We investigated the role of retinoic acid (RA) production by aldehyde dehydrogenase 1 (Aldh1a1, -a2, and -a3), the major RA-producing enzymes on sex-specific fat depot formation. (diabetesjournals.org)
  • CM10 is a potent and selective aldehyde dehydrogenase 1A (ALDH1A) family inhibitor, with IC 50 s of 1700, 740, and 640 nM for ALDH1A1, ALDH1A2, and ALDH1A3, respectively. (medchemexpress.com)
  • Conversely, aldehyde dehydrogenase activation using retinoic acid added to control conjunctival fibroblasts cultures promoted OcMMP-like phenotype in the scarring assays. (bl.uk)
  • Finally, the three retinaldehyde dehydrogenases cooperatively mediate retinoic acid signaling during the eye development. (ovid.com)
  • The protein may also be known as SSADH, SSDH, succinate-semialdehyde dehydrogenase, mitochondrial, and NAD(+)-dependent succinic semialdehyde dehydrogenase. (biocompare.com)
  • aldh5a1 has several biochemical functions, for example, aldehyde dehydrogenase (NAD) activity, protein homodimerization activity, succinate-semialdehyde dehydrogenase (NAD+) activity. (creativebiomart.net)
  • A fluorimetric assay of aldehyde dehydrogenase isozymes, based on naphthaldehyde oxidation, is compared with Western Blotting analysis on several clinical samples obtained from surgery. (mdpi.com)
  • Characterization of aldehyde dehydrogenase isozymes in ovarian cancer tissues and sphere cultures. (harvard.edu)
  • Holmes, R.S., 1978b, Genetics and ontogeny of aldehyde dehydrogenase isozymes in the mouse: localization of Ahd-1 encoding the mitochondrial isozymes on chromosome 4, Biochem. (springer.com)
  • Holmes, R. S., 1979b, Genetics, ontogeny and testosterone inducibility of aldehyde oxidase isozymes in the mouse: evidence for two genetic loci (Aox-1 and Aox-2) closely linked on chromosome 1, Biochem. (springer.com)
  • There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. (creative-biogene.com)
  • Other names in common use include betaine aldehyde oxidase, BADH, betaine aldehyde dehydrogenase, and BetB. (wikipedia.org)
  • Aldehyde oxidase ( EC:1.2.3.1 ) catalyses the conversion of an aldehyde in the presence of oxygen and water to an acid and hydrogen peroxide. (ebi.ac.uk)
  • The aldehyde oxidase and xanthine dehydrogenase, a/b hammerhead domain is an evolutionary conserved protein domain [ PMID: 7502041 , PMID: 10430865 ]. (ebi.ac.uk)
  • Crystal structure of the xanthine oxidase-related aldehyde oxido-reductase from D. gigas. (ebi.ac.uk)
  • Ahd-1 (encoding AHD-A 2 ) was found on chromosome 4 near Gpd-1 (encoding the liver isozyme of glucose-6-phosphate dehydrogenase), whereas the aldehyde oxidase loci (Aox-1, Aox-2) were closely linked on chromosome 1 near Id-1 (encoding isocitrate dehydrogenase). (springer.com)
  • Here we describe the immobilisation of two biocatalytically relevant co-factor recycling enzymes, glucose dehydrogenase (GDH) and NADH oxidase (NOD) on aldehyde functional ReSynTM polymer microspheres with varying functional group densities. (csir.co.za)
  • Disulfiram labeled with carbon-14 reacts specifically with human liver aldehyde dehydrogenase E1 with loss of catalytic activity and no incorporation of label. (sciencemag.org)
  • The subcellular distribution and properties of four aldehyde dehydrogenase isoenzymes (I-IV) identified in 2-acetylaminofluorene-induced rat hepatomas and three aldehyde dehydrogenases (I-III) identified in normal rat liver are compared. (portlandpress.com)
  • In normal liver, mitochondria (50%) and microsomal fraction (27%) possess the majority of the aldehyde dehydrogenase, with cytosol possessing little, if any, activity. (portlandpress.com)
  • The quantitative change involves both an increase in activity and a change in subcellular location of a basal normal-liver aldehyde dehydrogenase isoenzyme. (portlandpress.com)
  • Steady-state and pre-steady kinetic studies on mitochondrial sheep liver aldehyde dehydrogenase. (biochemj.org)
  • Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. (mybiosource.com)
  • 4-HNE is an α , β -unsaturated aldehyde formed during lipid peroxidation in vivo [ 17 ]. (hindawi.com)
  • Aldehyde dehydrogenases (ALDHs) are responsible for oxidation of biogenic aldehyde intermediates as well as for cell detoxification of aldehydes generated during lipid peroxidation. (biochemj.org)
  • Several researches done in the past have proven that Aldehyde Dehydrogenases are able to oxidize a wide range of endogenous and exogenous aldehydes. (steadyhealth.com)
  • They convert aldehydes (R-C(=O)-H) to carboxylic acids (R-C(=O)-O-H). The oxygen comes from a water molecule. (wikipedia.org)
  • We offer Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody Pairs for use in common research applications: Immunoprecipitation, Sandwich ELISA, Western Blot. (novusbio.com)
  • Each Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody Pair is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. (novusbio.com)
  • Our Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody Pairs can be used in a variety of model species: Human. (novusbio.com)
  • Choose from our Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody Pairs. (novusbio.com)
  • Enzymatic activity analysis showed that TraeALDH7B1-5A had acetaldehyde dehydrogenase activity. (springer.com)
  • A cysteine and a glutamate will interact with the aldehyde substrate. (wikipedia.org)
  • Cys302 and Glu268 interact with the aldehyde substrate. (wikipedia.org)
  • There is no doubt- aldehyde dehydrogenases have broad substrate specificity. (steadyhealth.com)
  • Isoenzymes I-III can be identified in both fractions and differ from each other on the basis of substrate and coenzyme specificity, substrate K m , inhibition by disulfiram and anti-(hepatoma aldehyde dehydrogenase) sera, and/or isoelectric point. (portlandpress.com)
  • Oxidation of aldehydes is considered to be generally a detoxification reaction because it is based on removing the electrophilic products of alcohol oxidation. (steadyhealth.com)
  • It is proven that alcohol dehydrogenase oxidizes ethanol to acetaldehyde, which is responsible for some hangover symptoms. (steadyhealth.com)
  • There is a single major alcohol dehydrogenase (ADH) and a single major aldehyde dehydrogenase (AldDH) in Aspergillus nidulans . (royalsocietypublishing.org)
  • Below are the list of possible Aldehyde-alcohol dehydrogenase products. (mybiosource.com)
  • Alcohol and aldehyde dehydrogenases: Retinoid metabolic effects in mouse knockout models. (ozgene.com)
  • The first step of RA synthesis is controlled by enzymes of the alcohol dehydrogenase (ADH) and retinol dehydrogenase (RDH) families that catalyze oxidation of retinol to retinaldehyde. (ozgene.com)
  • Aldehyde Dehydrogenase 1A1: Friend or Foe to Female Metabolism? (mdpi.com)
  • Endogenous aldehydes are generated during the metabolism of amino acids, carbohydrates, lipids, and vitamins as well as the biotransformation of many drugs and environmental chemicals [ 10 , 13 - 15 ]. (hindawi.com)
  • It is important to know that aldehydes are substances formed during the metabolism of amino acids, carbohydrates, lipids, biogenic amines, vitamins, and steroids. (steadyhealth.com)
  • Glutaryl-CoA dehydrogenase (ThnN) would catalyze the oxidation and decarboxylation of glutaryl-CoA and yield crotonyl-CoA, which enters the central metabolism via acetyl-CoA. (sigmaaldrich.com)
  • Phylogenetically, the Cp- and Ath-ALDH3 and -ALDH4 proteins are closely related to aldehyde dehydrogenases from bacteria and mammalian species and are separated from known plant ALDHs and betaine-aldehyde dehydrogenases (BADH). (ingentaconnect.com)
  • Catalysis of the reaction: an aldehyde + NAD+ + H2O = an acid + NADH + H+. (yeastgenome.org)
  • Aldehyde dehydrogenases (ALDHs) are highly expressed in the chemotherapy- and radiotherapy-resistant cell subpopulations of many different cancer types. (nih.gov)
  • Thus, the detoxification of harmful aldehydes generated endogenously or ingested from environment and foods is an important role of ALDHs. (hindawi.com)
  • Therefore, ALDHs play a crucial role in detoxifying aldehydes produced by various metabolic pathways. (biochemj.org)
  • Addition of disulfiram, a selective aldehyde dehydrogenase inhibitor restored phenotype and function of OcMMP fibroblasts to control cell levels. (bl.uk)
  • The data indicate that the tumour-specific aldehyde dehydrogenase phenotype is explainable by qualitative and quantitative changes involving primarily cytosolic and microsomal aldehyde dehydrogenase. (portlandpress.com)
  • This protein belongs to the aldehyde dehydrogenases family of proteins. (nih.gov)
  • On the other hand, aldehydes are highly reactive compounds, which can form adducts with proteins, DNA, and lipids, affecting the function of these biomolecules and leading to cell toxicity. (hindawi.com)
  • Down-regulation of aldehyde dehydrogenase 1A1 in Clone #3 CM and gelsolin levels in Clone #8 CM by siRNA transfection revealed an important involvement of these proteins in promoting and inhibiting invasion in these pancreatic cancer cell lines. (dcu.ie)
  • Ogier G, Chantepie J, Quash G, Doutheau A, Gord J, Marion C: The effect of a novel inhibitor of aldehyde dehydrogenase on viral replication. (springer.com)
  • It is also important in metabolizing other toxic aldehydes such as 4-hydroxy-2-nonenal (4-HNE) and acrolein [ 2 ]. (hindawi.com)
  • In animal myocardial or cerebral ischemia/ reperfusion (I/R) models, accumulation of toxic aldehydes, such as 4-hydroxy-2-nonenal and malondialdehyde, is thought to be an important mechanism for myocardial and cerebral I/R injury. (eurekaselect.com)
  • Aldehyde dehydrogenase activity promotes survival of human muscle precursor cells. (nih.gov)
  • Aldehyde dehydrogenase activity in xenografted human brain tumor in nude mice. (springer.com)
  • Koivula T, Turner AJ, Huttunen M, Koivusalo M: Subcellular and perisynaptic distribution of rat brain aldehyde dehydrogenase activity. (springer.com)
  • Harvey WK, Lindahl R: Activity of various aldehyde-metabolizing enzymes in chemically-induced rat hepatomas. (springer.com)
  • Perin A, Sessa A, Ciaranfi E: Carcinostatic effect of aliphatic aldehydes and aldehyde dehydrogenase activity in Ehrlich carcinoma, Sarcoma 180, and Yoshida AH 130 hepatoma. (springer.com)
  • ThnG has a nonacylating NAD-dependent pimelic semialdehyde dehydrogenase activity that renders pimelic acid a seven-carbon dicarboxylic acid. (sigmaaldrich.com)
  • Mutagenesis studies have shown that these genes are not essential for growth on tetralin or fatty acids, although a thnG disruption mutant showed threefold less pimelic semialdehyde dehydrogenase activity. (sigmaaldrich.com)
  • Hepatomas possess considerable cytosolic aldehyde dehydrogenase (20%), in addition to mitochondrial (23%) and microsomal (35%) activity. (portlandpress.com)
  • Nolta, Jan A. / Widespread nonhematopoietic tissue distribution by transplanted human progenitor cells with high aldehyde dehydrogenase activity . (elsevier.com)
  • Inoue K, Lindros KO: Subcellular distribution of human brain aldehyde dehydrogenase. (springer.com)
  • Canuto RA, Garcea R, Biocca M, Pascale R, Pirisi L, Feo F: The subcellular distribution and properties of aldehyde dehydrogenase of hepatoma AH-130. (springer.com)
  • This fatty aldehyde dehydrogenase deficiency is in fact a genetic disease. (steadyhealth.com)
  • Restoration of fatty aldehyde dehydrogenase deficiency in Sjögren-Larsson syndrome. (drugster.info)
  • Aldehyde dehydrogenases (EC 1.2.1.3) are a group of enzymes that catalyse the oxidation of aldehydes. (wikipedia.org)
  • Well, it is very important to know that aldehyde dehydrogenases are also a group of enzymes that catalyse the oxidation or dehydrogenation of aldehydes. (steadyhealth.com)
  • One theory is that a certain type of stem cell, aldehyde dehydrogenase bright stem cells, may stimulate the growth of new vessels. (clinicaltrials.gov)
  • Molecular cloning of the mature NAD(+)-dependent succinic semialdehyde dehydrogenase from rat and human. (wikidoc.org)
  • Human succinic semialdehyde dehydrogenase. (wikidoc.org)
  • Your search returned 21 aldehyde dehydrogenase 1 family member L1 ELISA ELISA Kit across 3 suppliers. (biocompare.com)
  • Anti-Aldehyde dehydrogenase 5 family member A1 antibodies are available from several suppliers. (biocompare.com)
  • Your search returned 192 aldehyde dehydrogenase 5 family member A1 Antibodies across 28 suppliers. (biocompare.com)
  • Recombinant ABA2 protein produced in Escherichia coli exhibits a K m value for xanthoxin of 19 μM and catalyzes in a NAD-dependent manner the conversion of xanthoxin to abscisic aldehyde, as determined by HPLC-mass spectrometry. (plantcell.org)
  • Aldehyde Dehydrogenase, Yeast, CAS 9028-88-0, is a native aldehyde dehydrogenase from yeast that catalyzes the oxidation of acetaldehyde to acetic acid. (merckmillipore.com)
  • Aldehydes, which are highly reactive molecules, are toxic at high concentrations. (biochemj.org)
  • These results strongly suggest that elevated reactive aldehyde concentration, like that observed in the presence of chronic pain, may render cardiomyocytes more susceptible to I/R injury by SIRT1 carbonylative inactivation and impairment the cardioprotection of LKB1-mediated AMPK activation. (onlinejacc.org)