A copper-containing dye used as a gelling agent for lubricants, for staining of bacteria and for the dyeing of histiocytes and fibroblasts in vivo.
A histochemical technique for staining carbohydrates. It is based on PERIODIC ACID oxidation of a substance containing adjacent hydroxyl groups. The resulting aldehydes react with Schiff reagent to form a colored product.
Heteropolysaccharides which contain an N-acetylated hexosamine in a characteristic repeating disaccharide unit. The repeating structure of each disaccharide involves alternate 1,4- and 1,3-linkages consisting of either N-acetylglucosamine or N-acetylgalactosamine.
The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.
Chemicals and substances that impart color including soluble dyes and insoluble pigments. They are used in INKS; PAINTS; and as INDICATORS AND REAGENTS.
Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.
A phenothiazine that has been used as a hemostatic, a biological stain, and a dye for wool and silk. Tolonium chloride has also been used as a diagnostic aid for oral and gastric neoplasms and in the identification of the parathyroid gland in thyroid surgery.
A subcategory of mucins that contain SIALIC ACID.
A condition in which there is a change of one adult cell type to another similar adult cell type.
High molecular weight mucoproteins that protect the surface of EPITHELIAL CELLS by providing a barrier to particulate matter and microorganisms. Membrane-anchored mucins may have additional roles concerned with protein interactions at the cell surface.
The viscous secretion of mucous membranes. It contains mucin, white blood cells, water, inorganic salts, and exfoliated cells.
The formation of cartilage. This process is directed by CHONDROCYTES which continually divide and lay down matrix during development. It is sometimes a precursor to OSTEOGENESIS.
Xanthene dye used as a bacterial and biological stain. Synonyms: Pyronin; Pyronine G; Pyronine Y. Use also for Pyronine B. which is diethyl-rather than dimethylamino-.
Acids derived from monosaccharides by the oxidation of the terminal (-CH2OH) group farthest removed from the carbonyl group to a (-COOH) group. (From Stedmans, 26th ed)
A non-vascular form of connective tissue composed of CHONDROCYTES embedded in a matrix that includes CHONDROITIN SULFATE and various types of FIBRILLAR COLLAGEN. There are three major types: HYALINE CARTILAGE; FIBROCARTILAGE; and ELASTIC CARTILAGE.
Mucins that are found on the surface of the gastric epithelium. They play a role in protecting the epithelial layer from mechanical and chemical damage.
A natural high-viscosity mucopolysaccharide with alternating beta (1-3) glucuronide and beta (1-4) glucosaminidic bonds. It is found in the UMBILICAL CORD, in VITREOUS BODY and in SYNOVIAL FLUID. A high urinary level is found in PROGERIA.
Polymorphic cells that form cartilage.
A glandular epithelial cell or a unicellular gland. Goblet cells secrete MUCUS. They are scattered in the epithelial linings of many organs, especially the SMALL INTESTINE and the RESPIRATORY TRACT.
Granulated cells that are found in almost all tissues, most abundantly in the skin and the gastrointestinal tract. Like the BASOPHILS, mast cells contain large amounts of HISTAMINE and HEPARIN. Unlike basophils, mast cells normally remain in the tissues and do not circulate in the blood. Mast cells, derived from the bone marrow stem cells, are regulated by the STEM CELL FACTOR.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
A salivary gland on each side of the mouth below the TONGUE.
Derivatives of chondroitin which have a sulfate moiety esterified to the galactosamine moiety of chondroitin. Chondroitin sulfate A, or chondroitin 4-sulfate, and chondroitin sulfate C, or chondroitin 6-sulfate, have the sulfate esterified in the 4- and 6-positions, respectively. Chondroitin sulfate B (beta heparin; DERMATAN SULFATE) is a misnomer and this compound is not a true chondroitin sulfate.
Glands of external secretion that release its secretions to the body's cavities, organs, or surface, through a duct.
Carbohydrates covalently linked to a nonsugar moiety (lipids or proteins). The major glycoconjugates are glycoproteins, glycopeptides, peptidoglycans, glycolipids, and lipopolysaccharides. (From Biochemical Nomenclature and Related Documents, 2d ed; From Principles of Biochemistry, 2d ed)
A fibrillar collagen found predominantly in CARTILAGE and vitreous humor. It consists of three identical alpha1(II) chains.
Glycoproteins which have a very high polysaccharide content.
Diseases of the domestic dog (Canis familiaris). This term does not include diseases of wild dogs, WOLVES; FOXES; and other Canidae for which the heading CARNIVORA is used.
Methods of preparing tissue for examination and study of the origin, structure, function, or pathology.
An enzyme that catalyzes the random hydrolysis of 1,4-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate. (From Enzyme Nomenclature, 1992) There has been use as ANTINEOPLASTIC AGENTS to limit NEOPLASM METASTASIS.
Large HYALURONAN-containing proteoglycans found in articular cartilage (CARTILAGE, ARTICULAR). They form into aggregates that provide tissues with the capacity to resist high compressive and tensile forces.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
Tissue that supports and binds other tissues. It consists of CONNECTIVE TISSUE CELLS embedded in a large amount of EXTRACELLULAR MATRIX.
A protective layer of firm, flexible cartilage over the articulating ends of bones. It provides a smooth surface for joint movement, protecting the ends of long bones from wear at points of contact.
A diazo-naphthalene sulfonate that is widely used as a stain.
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
A plant genus of the family TILIACEAE. Some species in this genus are called Limetree which is nearly the same as the common name for lime (CITRUS AURANTIIFOLIA). Some people are allergic to the POLLEN.
An isomer of 1-PROPANOL. It is a colorless liquid having disinfectant properties. It is used in the manufacture of acetone and its derivatives and as a solvent. Topically, it is used as an antiseptic.
An undecenyl THIOUREA which may have topical anti-inflammatory activity.
A genus of fungi in the family Ophiostomataceae, order OPHIOSTOMATALES. Several species are the source of Dutch elm disease, which is spread by the elm bark beetle.
Thin outer membrane that surrounds a bone. It contains CONNECTIVE TISSUE, CAPILLARIES, nerves, and a number of cell types.
Differentiated epithelial cells of the INTESTINAL MUCOSA, found in the basal part of the intestinal crypts of Lieberkuhn. Paneth cells secrete GROWTH FACTORS, digestive enzymes such as LYSOZYME and antimicrobial peptides such as cryptdins (ALPHA-DEFENSINS) into the crypt lumen.
The branch of physics which deals with the motions of material bodies, including kinematics, dynamics, and statics. When the laws of mechanics are applied to living structures, as to the locomotor system, it is referred to as BIOMECHANICAL PHENOMENA. (From Dorland, 28th ed)
The process of bone formation. Histogenesis of bone including ossification.
Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.
An umbrella term used to describe a pattern of disabilities and abnormalities that result from fetal exposure to ETHANOL during pregnancy. It encompasses a phenotypic range that can vary greatly between individuals, but reliably includes one or more of the following: characteristic facial dysmorphism, FETAL GROWTH RETARDATION, central nervous system abnormalities, cognitive and/or behavioral dysfunction, BIRTH DEFECTS. The level of maternal alcohol consumption does not necessarily correlate directly with disease severity.
Bone-marrow-derived, non-hematopoietic cells that support HEMATOPOETIC STEM CELLS. They have also been isolated from other organs and tissues such as UMBILICAL CORD BLOOD, umbilical vein subendothelium, and WHARTON JELLY. These cells are considered to be a source of multipotent stem cells because they include subpopulations of mesenchymal stem cells.
Information or data used to ensure the safe handling and disposal of substances in the workplace. Such information includes physical properties (i.e. melting, boiling, flashing points), as well as data on toxicity, health effects, reactivity, storage, disposal, first-aid, protective equipment, and spill-handling procedures.
A state in southeastern Australia. Its capital is Sydney. It was discovered by Captain Cook in 1770 and first settled at Botany Bay by marines and convicts in 1788. It was named by Captain Cook who thought its coastline resembled that of South Wales. (From Webster's New Geographical Dictionary, 1988, p840 & Room, Brewer's Dictionary of Names, 1992, p377)
An educational process that provides information and advice to individuals or families about a genetic condition that may affect them. The purpose is to help individuals make informed decisions about marriage, reproduction, and other health management issues based on information about the genetic disease, the available diagnostic tests, and management programs. Psychosocial support is usually offered.
Elements, compounds, mixtures, or solutions that are considered severely harmful to human health and the environment. They include substances that are toxic, corrosive, flammable, or explosive.

Changes in joint cartilage aggrecan after knee injury and in osteoarthritis. (1/131)

OBJECTIVE: To determine the concentrations of aggrecan fragments in synovial fluid from patients with knee joint injury, osteoarthritis (OA), or acute pyrophosphate arthritis (PPA; pseudogout), and to test their relative reactivity with the 846 epitope, a putative marker of cartilage aggrecan synthesis. METHODS: Samples of knee joint fluid from 385 patients and 9 healthy-knee volunteers were obtained in a cross-sectional study. Study groups were acute PPA/ pseudogout (n = 60), anterior cruciate ligament (ACL) rupture (n = 159), meniscus lesion (n = 129), and primary knee OA (n = 37). The 846 epitope on aggrecan was assayed by competitive solution-phase radioimmunoassay. Aggrecan fragments were assayed by enzyme-linked immunosorbent assay using a monoclonal antibody (1-F21). Cartilage oligomeric matrix protein (COMP), C-propeptide of type II collagen (CPII), bone sialoprotein, matrix metalloproteinases 1 and 3, and tissue inhibitor of metalloproteinases 1 were previously quantified by immunoassays. RESULTS: Reactivity of the 846 epitope was increased in all study groups compared with the reference group, and was highest in patients with primary OA. The median levels (in microg fetal aggrecan equivalents/ml) of the epitope were 0.28 (range 0.24-0.47) in the reference group, 0.48 (range 0.26-1.32) in PPA/pseudogout, 0.61 (range 0.12-2.87) in ACL rupture, 0.53 (range 0.22-3.02) in meniscus lesion, and 0.68 (range 0.31-4.31) in primary OA. The 846 epitope reactivity per microg aggrecan fragments in the joint fluid was higher in late-stage OA than in early-stage OA. Epitope 846 reactivity correlated positively with several markers of matrix turnover, particularly with COMP (r(s) = 0.421) and CPII (r(s) = 0.307). CONCLUSION: The observed differences in 846 epitope reactivity in synovial fluid, and its concentration in relation to aggrecan and other markers of matrix turnover, were consistent with marked ongoing changes in aggrecan turnover after joint injury and in the development of OA. OA is thus a disease characterized by dynamic changes in tissue macromolecule turnover, which is reflected by measurable changes in aggrecan epitopes in the synovial fluid.  (+info)

Differences in the acid-labile component of Candida albicans mannan from hydrophobic and hydrophilic yeast cells. (2/131)

Cell surface hydrophobicity of the opportunistic fungal pathogen Candida albicans has been linked to the level of cell wall protein glycosylation. Previous work demonstrated that outer chain mannosylation, rather than overall glycosylation, correlated with cell surface hydrophobicity. These studies further suggested that the phosphodiester-linked, acid-labile beta-1,2-mannan was the correlating element. The present work tests this hypothesis and extends the previous results. The composition of bulk mannan from hydrophobic and hydrophilic yeast cells, and the acid-labile mannan from both cell types are compared. Compositional analysis shows that the protein, hexose, and phosphorus content of bulk mannan is similar between the two phenotypes. Electrophoretic separation of acid-released and fluorophore-labeled mannan shows that the acid-labile oligomannosides from hydrophobic cells are longer and potentially in greater abundance than those from hydrophilic cells. These results suggest that regulation of a single step in cell wall protein outer chain mannosylation affects the cell surface ultrastructure and phenotype of C.albicans.  (+info)

Induction of dog sperm capacitation by glycosaminoglycans and glycosaminoglycan amounts of oviductal and uterine fluids in bitches. (3/131)

Ejaculated sperm collected from 12 beagle dogs were incubated in canine capacitation medium (CCM), supplemented with 5 microg/ml chondroitin sulfate A (CS), 5 microg/ml hyaluronic acid (HA), or 5 microg/ml heparin (HP) for 7 hr at 38 degrees C in a 5% CO2 in air atmosphere to investigate the effects of glycosaminoglycans (GAGs) on dog sperm capacitation. The percentages of motile sperm, hyperactivated sperm (%HY), and acrosome-reacted sperm (%AR) in all media were examined after 4 hr and 7 hr of incubation. The oviducts and uteri of 9 anestrous and 18 estrous beagle bitches were removed under halothane inhalation anesthesia to measure the total GAG amounts in oviductal and uterine fluids. The lumens of the ampulla of the oviducts, isthmus of the oviducts, and the uterine horns were each flushed with 1 ml HEPES-EDTA fluid. Total GAG amounts in the flush fluids obtained were measured with a spectrophotometer. Sperm motility (51-59%), %HY (79-86%), and %AR (31-36%) in CCM supplemented with CS, HA, or HP were significantly higher after 7 hr of incubation than when incubated in CCM without GAGs (P<0.01 or 0.05). The mean total GAG amounts in the fluids from the ampulla and isthmus of the oviducts and the uterine horns in the estrous bitches were higher than in the anestrous bitches. These results indicate that GAGs in the oviductal and uterine fluids in estrous bitches are associated with in vivo sperm capacitation.  (+info)

Mapping metastases in sentinel lymph nodes of breast cancer. (4/131)

Localization of metastases within the sentinel lymph nodes (SLNs) of breast cancer has not been studied. Forty SLNs from 36 patients with operable primary breast cancers were identified by means of lymphatic mapping with patent blue dye. The junction between the patent blue-stained lymphatic vessel draining the tumor and the SLN was labeled with alcian blue. Metastases within the serially sectioned SLNs were assigned to the alcian blue-labeled side, to the opposite side of the virtually halved nodes, or both. Eight SLNs were negative for metastasis. Eleven SLNs had metastases only in the blue half. Only 4 cases had larger metastases in the nonblue half. Metastases are more likely to be located in the vicinity of the inflow junction of the identifiable lymphatic draining the tumor and the SLN. This should be considered when SLNs are examined, especially when they are halved for different studies.  (+info)

The pathogenesis of duodenal gastric metaplasia: the role of local goblet cell transformation. (5/131)

BACKGROUND AND AIMS: Gastric metaplasia is frequently seen in biopsies of the duodenal cap, particularly when inflamed or ulcerated. In its initial manifestation small patches of gastric foveolar cells appear near the tip of a villus. These cells contain periodic acid-Schiff (PAS) positive neutral mucins in contrast with the alcian blue (AB) positive acidic mucins within duodenal goblet cells. Previous investigations have suggested that these PAS positive cells originate either in Brunner's gland ducts or at the base of duodenal crypts and migrate in distinct streams to the upper villus. To investigate the origin of gastric metaplasia in superficial patches, we used the PAS/AB stain to distinguish between neutral and acidic mucins and in addition specific antibodies to immunolocalise foveolar cell mucin MUC5AC, the foveolar cell secretory product, gastric trefoil factor (TFF1), the mature goblet cell mucin MUC2, and MUC2 core antigen. RESULTS: Cells in focal patches of gastric metaplasia contained secretory granules of both gastric and goblet cell phenotypes. MUC5AC and TFF1 were present as expected in gastric foveolar cells but in addition, MUC2 core antigen, normally present only in the Golgi of intestinal goblet cells, was expressed in secretory granules. Goblet cells in the vicinity of metaplastic patches also expressed both gastric and intestinal antigens. MUC5AC/MUC2 containing goblet cells were most common near the villus tip but were also seen at the base of crypts. Where crypts and Brunner's gland ducts merged they were always seen on the crypt side of the junction. Goblet cells were the only cells to express gastric antigens in these areas. In advanced metaplastic lesions, dual phenotype goblet cells were less evident and fewer cells expressed intestinal mucin antigens. CONCLUSIONS: We suggest that goblet cells that express both intestinal and gastric antigens may represent local precursors of gastric metaplasia undergoing a transition to foveolar-like cells of mixed phenotype at the site of early metaplastic patches. As metaplasia becomes more widespread, a more pure gastric phenotype emerges. This progression is likely to be controlled by local inflammatory signals.  (+info)

Development of conjunctival goblet cells and their neuroreceptor subtype expression. (6/131)

PURPOSE: To investigate expression of muscarinic, cholinergic, and adrenergic receptors on developing conjunctival goblet cells. METHODS: Eyes were removed from rats 9 to 60 days old, fixed, and used for microscopy. For glycoconjugate expression, sections were stained with Alcian blue/periodic acid-Schiffs reagent (AB/PAS) and with the lectins Ulex europeus agglutinin I (UEA-I) and Helix pomatia agglutinin (HPA). Goblet cell bodies were identified using anti-cytokeratin 7 (CK7). Nerve fibers were localized using anti-protein gene product 9.5. Location of muscarinic and adrenergic receptors was investigated using anti-muscarinic and beta-adrenergic receptors. RESULTS: At days 9 and 13, single apical cells in conjunctival epithelium stained with AB/PAS, UEA-I, and CK7. At days 17 and 60, increasing numbers of goblet cells were identified by AB/PAS, UEA-I, HPA, and CK7. Nerve fibers were localized around stratified squamous cells and at the epithelial base at days 9 and 13, and around goblet cells and at the epithelial base at days 17 and 60. At days 9 and 13, M2- and M3-muscarinic and beta2-adrenergic receptors were found in stratified squamous cells, but M1-muscarinic and beta1-adrenergic receptors were not detected. At days 17 and 60, M2- and M3-muscarinic receptors were found in goblet cells, whereas M1-muscarinic receptors were in stratified squamous cells. Beta1- and beta2-adrenergic receptors were found on both cell types. Beta3-adrenergic receptors were not detected. CONCLUSIONS: In conjunctiva, nerves, M2- and M3-muscarinic, and beta1- and beta2-adrenergic receptors are present on developing goblet cells and could regulate secretion as eyelids open.  (+info)

Dynamic regulation of mucus gel thickness in rat duodenum. (7/131)

We examined the dynamic regulation of mucus gel thickness (MGT) in vivo in rat duodenum in response to luminal acid, cyclooxygenase (COX) inhibition, and exogenous PGE(2). An in vivo microscopic technique was used to measure MGT with fluorescent microspheres in urethan-anesthetized rats. Duodenal mucosa was topically superfused with pH 7.0 or pH 2.2 solutions with or without PGE(2) and indomethacin treatments. Glycoprotein concentration of duodenal loop perfusates was measured with periodic acid/Schiff (PAS) or Alcian blue (AB) staining. MGT and perfusate glycoprotein concentration were stable during a 35-min perfusion with pH 7.0 solution. Acid exposure increased MGT and PAS- and AB-positive perfusate glycoprotein concentrations. Indomethacin pretreatment increased both PAS- and AB-positive perfusate glycoprotein at baseline; subsequent acid superfusion decreased perfusate glycoproteins and gel thickness. PGE(2) (1 mg/kg iv) simultaneously increased MGT and PAS-positive perfusate glycoprotein concentrations followed by a transient increase in AB-positive glycoprotein concentration, suggesting contributions from goblet cells and Brunner's glands. Parallel changes in MGT and perfusate glycoprotein concentration in response to luminal acid and PGE(2) suggest that rapid MGT variations reflect alterations in the balance between mucus secretion and exudation, which in turn are regulated by a COX-related pathway. Luminal acid and PGE(2) augment mucus secretion from goblet cells and Brunner's glands.  (+info)

Genetic control of yeast mannan structure: mapping genes mnn2 and mnn4 in Saccharomyces cerevisiae. (8/131)

Two mutations concerned with mannan biosynthesis in the yeast Saccharomyces cerevisiae have been mapping. The mnn2 mutation, which affects the addition to the polysaccharide backbone of the first side-chain D-mannose unit in alpha1-leads to2 linkage, was located on chromosome II linked to the centromere and the gall locus. The mn4 locus, which regulates the synthesis of mannosylphosphate groups on the mannan side chains, was placed on chromosome XI near trp3 and ural and a locus previously reported to regulate the ability of a S. diastaticus strain to bind alcian blue (Friis and Ottolenghi, 1970). The mnn4 mutant also fails to bind alcain blue, but the gene responsible for alcian blud binding in this strain segregates independently from the dye-binding locus of S. diastaticus, and therefore must be a different gene. A diploid heterozygous for mnn4 fails to bind dye, indicating dominance of this mutant genotype. The alcian blue dye binding locus dbll, reported to Friis and Ottolenghi (1970), is also dominant. Thus, there are at least two independent genes that control the formation of the mannosylphosphate units in the mannan side chains, and both have the property of dominance in the mutant form.  (+info)

0, and good lipid stains generally have a LogP>+7). Methanol is an acceptable substitute for ethanol as a potential vehicle for Alcian blue, but isopropanol is not, because, within a few hours all of suspended Alcian blue precipitates if isopropanol is tried as a vehicle. The sample compound with Merck index number 218 has a melting point of 148 °C. It is a tetravalent basic (cationic) dye with a copper (Cu2+, coordination 4 of 6, orbital configuration d9 with Jahn-Teller distortion) phthalocyanine nucleus (CuPc) with three or four pendent isothiouronium side chains imparting its bulkiness and positive charges. In order to qualify as an alcian blue family member there has to be at least 2 side chains and the mixtures often have 3 chains in average to qualify as 8G. Four tetramethylisothiouronium groups per molecule are shown in the picture. ICI had claimed an average of about three side chains per molecule, but analyses by Prof Scotts lab suggested between three and four. Most of them are at ...
Hi Tom: Alcian Blue will stain the glycosaminoglycans in the extracellular matrix, it wont show much cellular morphology. Still, the traditional method is 1% Alcian Blue in 3% acetic acid for a pH of 2.5. At 2.5 both sulfated and carboxylated GAGs are stained. A red nuclear stain, Scarba red or nuclear fast red, gives a nice contrasty counterstain. You might try 0.05% Toluidine blue in dist water with a pH of about 4.0 for 10-15 minutes, dist water rinse and dehydration in 3 changes of actone followed by clearing in fresh xylene. Nice metachromasia of=20 extracellular matrix and you will see some cellular morphology as well. Geoff Tom Schaer wrote: >Greetings List: > >I would like to stain some intervertebral disc x-sections (rat, decalcified) with alcian blue. Interest in cellular morphology of nucleus pulposus, transition and annulus cells. > >What pH of the stain should I use. > >Many thanks, > >tom >-------------------------------------------------- >Thomas P. Sch r, VMD >Asst Prof School ...
Fingerprint Dive into the research topics of Histochemical study of gastric mucosubstances after thermal injury: Correlation with endoscopic evidence of acute gastroduodenal disease. Together they form a unique fingerprint. ...
Thanks to all who responded to the Alcian blue question. It is definitely a developmental problem with these bones/cartilage. The cells are not producing the required muccopolysaccharides necessary for proper staining. anita ...
Demonstrate the presence of both neutral and acidic mucosubstances with the Thermo Scientific Richard-Allan Scientific Alcian Blue/PAS Special Stain Kit
And APPLE -GREEN BIREFRINGERENCE on POLARISED MICROSCOPY(DIAGNOSTIC). 9) ALCIAN BLUE Stains;-. #Mucin and mucosubstances. 10) ACID FAST Stains;-. #Mycobacterium. 11) FONTANA MASSON:-. #Melanin and Argentaffin cells.. 12) MASSONs TRICHROME Stains:-. #Collagen and connective tissue. 13) VERHOFF-VON-GIESON (VVG):-. #Elastic fibres ( other stain-ORCEIN stain). 14) TOLUIDINE BLUE:-. #Mast cells. 15) LUXOL FAST BLUE:-. #Myelin. 16) BIELSCHOWSKY Stains:-. (uses silver stain) ...
ABSTRACT: Microphytobenthos (MPB) are an important component of shallow-water habitats where there is sufficient light to support benthic photosynthesis. Production of extracellular polymeric substances (EPS) is characteristic of the MPB, and acid polysaccharides (APS) are a major component of these EPS. A simple technique was developed to extract and measure APS concentrations in sediments. Water-extractable APS (wAPS) were extracted with ultra high purity water from lyophilized sediments. After centrifuging to remove the sediment, wAPS in the supernatant was precipitated with alcian blue, a cationic dye. The APS-alcian blue precipitate was removed by filtration. The remaining alcian blue in solution was determined spectrophotometrically and was inversely proportional to the concentration of wAPS in the sediment. Chlorophyll (chl) a, carbohydrate fractions, and wAPS were measured in sediments from 5 contrasting sites in a microtidal salt marsh in the subtropics. wAPS concentrations, expressed ...
Posterior salivary glands. As the gland described above, posterior salivary glands are simple branched tubular glands. They are covered by a connective capsule with muscular fibers surrounding the glandular tubules like thin connections. Each tubule presents two zones formed by different cells. The first or proximal zone (A) consists of globular mucous cells with flattened basal nuclei (Figure 5b). The supranuclear cytoplasm contains high level of PAS (+) and alcian blue (+) pH = 3.5, vesicles denoting the presence of weakly sulphated glycosaminoglycans content. Cylindrical cells with a basal spherical nucleus surrounded by a basophilic cytoplasm are also seen. The second or distal zone (B) present cylindrical cells characterized by its weakly eosinophilic cytoplasm. The nucleus with basal or apical location is observed (Figure 5b). The PAS (+) and Alcian Blue (-) pH = 3.5 reaction in the cytoplasm, suggests the presence of neutral carbohydrates.. Animals kept in the aquarium without food for ...
PAVON, L. F.... Ultrastructural characterization of CD133(+) stem cells bound to superparamagnetic nanoparticles: possible biotechnological applications. JOURNAL OF MICROSCOPY 231 n.3 p. 374-383 SEP 2008. Journal article.
Scores of people around the country are protesting against Drax Plc on the day of its AGM today, with three simultaneous demonstrations in London, Liverpool and York. Campaigners are highlighting Draxs involvement in dirty energy, its contributions to… Read More. ...
INTRODUCTION: The clinical efficacy of infliximab (IFX) for induction of remission in both adults and children with active Crohns disease (CD) has been well documented. Recently, so-called deep remission defined as mucosal healing has become the ultimate endpoint of the most recent therapeutic advances for CD. However, endoscopic evidence of mucosal healing is not necessarily associated with histological evidence of suppression of inflammation. AIM: Since data on that issue are limited, especially in the paediatric population, the aim of this study was to assess the impact of induction therapy with IFX on deep microscopic remission in paediatric patients with CD ...
D: The skeleton of a wild-type mouse (right) and Wwtr1tm1Whun/Wwtr1tm1Whun mouse (left) embryos stained with Alizarin red and Alcian blue to stain bone (red) and cartilage (blue), respectively. Note the slightly shorter skeleton of the homozygous mouse embryo. G & H: Hematoxylin and eosin staining of longitudinal kidney sections from 8 week old wild-type (G) and homozygous mutant mouse (H), showing numerous cysts in the corticomedullary region of the homozygous mouse kidney. Magnification: x20 ...
FIG. 3. Whole mount in situ hybridization pattern of Xbap [cc: now called nkx3-1] (A, B, C, D, E, and F) and gsc (G, H, I, J, and K) and Alcian blue staining to visualize cartilage (L). All embryos were photographed at 701 magnification except for (D) at 601. (A) Lateral view of a stage 36 embryo showing both regions of facial staining and the region of gut expression (indicated by g in the figure). (B) Anterior view of the embryo pictured in (A). The paired anterior regions of expression lie on either side of the future mouth opening (M). (C) Dorsal view of a stage 37 embryo showing the forked appearance of the Xbap staining. (D) Lateral view of the left side of a stage 42 embryo showing staining of the anterior gut tube and facial staining. (E) Dorsal view of a stage 40 embryo. Xbap staining now marks crescent-shaped regions on either side of the future mouth opening (M). (F) Dorsal view of the embryo shown in (D). The region of staining corresponding to the palatoquadrate cartilage (PQ) is ...
Hyaluronate electophoresis - posted in Biochemistry: Hi everyone! I would like to ask if anyone has any experience in electrophoresis and detection of hyaluronate. I did PAGE of a digest mixture (consisting of HA chains of various lengths) according to numerous protocols (15% and 10% gel in TBE, linear gradient 20%-3% and 18%-1,5%, with samples in NaOAc, NH4HCO3, NaOAc+HCl, dH20 or TBE, with the voltage of 300V and 100V and detection by Alcian Blue in water, water+2% acetic acid or wat...
Free flashcards to help memorize facts about UND 363 Alcian Blue 1.0. Other activities to help include hangman, crossword, word scramble, games, matching, quizes, and tests.
Contexto - Esôfago de Barrett é caracterizado pela presença de células caliciformes. Entretanto, quando alcian blue é utilizado, outro tipo de células, chamadas células colunares azuis, estão frequentemente presentes no ...
The colour database is collected and created by the experts of Chemnet. There are more than 1,000 colours in this database about the colours physicochemical property, applications, synthetic methods and so on. It will be updated in time.
An assay for quantifying apoptotic (green), necrotic (red), and total (blue) cells within the same cell population by flow cytometry or microscopy.
The heatmaps are normalized by row. Light yellow cells indicate no/low expression, dark blue cells show the maximum expression. White cells lacking a border are missing values.). ...
The heatmaps are normalized by row. Light yellow cells indicate no/low expression, dark blue cells show the maximum expression. White cells lacking a border are missing values.). ...
Looking for online definition of Alcian blue in the Medical Dictionary? Alcian blue explanation free. What is Alcian blue? Meaning of Alcian blue medical term. What does Alcian blue mean?
Looking for online definition of Alcian yellow-toluidine blue Leung stain in the Medical Dictionary? Alcian yellow-toluidine blue Leung stain explanation free. What is Alcian yellow-toluidine blue Leung stain? Meaning of Alcian yellow-toluidine blue Leung stain medical term. What does Alcian yellow-toluidine blue Leung stain mean?
Hito Alcian Blue pH-2.5 OptimStain™ Kit is formulated for the staining of acid mucins, sulfated / carboxylated acid mucopolysaccharides and sulfated / carboxylated sialomucins (glycoproteins).
Dissolve the dye in the distilled water. Add the acid and mix well. Filter into the reagent bottle (keeps well).. References. 1. Bancroft, J.D. and Stevens, A. 1982Theory and Practice of Histological Techniques. 2nd edition.Churchill-Livingstone.. 2. Lillie, R.D. 1977. H.J. Conns Biological Stains, 9th edition Williams and Wilkins Pearse, A.G. Everson 1972. 3. Histochemistry, Theoretical and Applied Vol. 1, 3rd edition, p344 Churchill-Livingstone.. 4. Steedman, H.F. 1950 Alcian blue 8GS; A new stain for mucin. Quarterly Journal of Microscopic Science, Vol 91, p477-479.. 5. Ajax Chemicals 1982-89Material Safety Data Sheets Ajax Chemicals Pty Ltd, Auburn, New South Wales, Australia.. 6. National Society for Histotechnology, Carbohydrate Reference Stain Manual, 1st edition, 1989.. ...
Orcein-alcian blue staining, a new method for the simultaneous demonstration of sulphated and sialomucins in gastrointestinal epithelium was compared with the standard high iron diamine-alcian blue technique. Sections were oxidised with potassium permanganate and decolourised in oxalic acid. They were stained with orcein for four hours, differentiated for a few seconds in acid alcohol, and then counterstained with alcian blue for half to one minute. There was a good correlation of results between the two methods. Orcein-alcian blue is a safer, cheaper, and quicker method than high iron diamine-alcian blue which can be safely introduced into routine laboratories for the study of acid mucins in the gastrointestinal diseases.. ...
Images: Cuboidal lining , Glycogen , Aggressive serous cyst , Cysts in serous cystadenomas are composed of a single layer of clear cuboidal cells. These cells usually form flat sheets that line the cysts and only rarely form papillae that project into the cystic spaces. The neoplastic cells have clear cytoplasm because they contain abundant intracytoplasmic glycogen. They do not produce mucin. The nuclei are small, round, and have uniform chromatin and inconspicuous nucleoli. Atypia and mitoses are essentially absent. The central stellate scar and the stroma separating the cysts are composed mostly of relatively acellular collagenous connective tissues, but the stroma may contain entrapped islets of Langerhans and acini. Special stains will highlight the abundant glycogen and absence of mucin. A periodic acid Schiff (PAS) stain will be strongly positive and sensitive to diastase digestion. Stains for mucin, including a mucicarmine and alcian blue stains, will be negative, as will stains for ...
Periodic acid-Schiff (PAS) is a staining method used to detect polysaccharides such as glycogen, and mucosubstances such as glycoproteins, glycolipids and mucins in tissues. The reaction of periodic acid oxidizes the vicinal diols in these sugars, usually breaking up the bond between two adjacent carbons not involved in the glycosidic linkage or ring closure in the ring of the monosaccharide units that are parts of the long polysaccharides, and creating a pair of aldehydes at the two free tips of each broken monosaccharide ring. The oxidation condition has to be sufficiently regulated so as to not oxidize the aldehydes further. These aldehydes then react with the Schiff reagent to give a purple-magenta color. A suitable basic stain is often used as a counterstain. PAS diastase stain (PAS-D) is PAS stain used in combination with diastase, an enzyme that breaks down glycogen. Alcian blue/periodic acid-Schiff (AB/PAS or AB-PAS) uses alcian blue before the PAS step. PAS staining is mainly used for ...
TY - JOUR. T1 - A Review on Adsorption of Cationic Dyes using Activated Carbon. AU - Corda, Nikita Chrishel. AU - Kini, M. Srinivas. PY - 2018/1/9. Y1 - 2018/1/9. N2 - In this article efficiency of activated carbon as a potent adsorbent of cationic dyes has been reviewed. Non-biodegradable nature of pollutants and their removal in the present generation is a great challenge. Therefore, extensive study on adsorption of these classes of pollutants from water bodies is being carried out. Methylene blue (majorly) a dye seen in the effluent streams of textile, printing, paper industries along with some of the commonly used cationic dyes in process industries and their sorption on activated carbon are reviewed here. High cost of commercially activated carbon which is a limitation to its extensive use have paved way for study of adsorption by naturally obtained and extracted activated carbon from agricultural wastes and various other sources. The purpose of this review paper is to summarize the ...
Grossly, the specimen was an ovoid cystic nodule measuring 1.1 × 0.6 × 0.6 cm and was covered by an ellipse of normal skin. The outer surface of the nodule was smooth and glistening. Upon sectioning, the cyst was well circumscribed, but not encapsulated (Fig. 1A) and was filled with pale tan, gelatinous material surrounded by grayish white fibrous tissue (Fig. 1B). Histologically, the lesion consisted of abundant myxoid tissue with a few scattered spindle cells and had a slightly multilobular appearance with fibrocollagenous stroma. Capillaries and thin-walled vessels were noted within the nodule (Fig. 1C). Rare histiocytes, lymphocytes, and plasma cells were seen in the nodule. Neither mammary ducts nor epithelial elements were observed. Spindle cells showed bland-looking nuclei and elongated amphophilic cytoplasm with no atypia or mitosis. The myxoid tissue was analyzed via Alcian blue staining (pH 2.5) and periodic acid-Schiff (PAS) testing. Alcian blue stained the myxoid stroma bright blue ...
We have developed a reliable and sensitive immunohistochemical staining technique which allows the simultaneous demonstration of two different antigens expressed in or on the same cell (referred to as mixed labeling), together with the evaluation of the general histopathological appearance of the tissue. The staining procedure combines a three-step (streptavidin-biotin) immunogold-silver staining (IGSS) with a three-step immunoenzymatic labeling. For this purpose, we investigated the compatibility ofIGSS with various substrates of peroxidase or alkaline phosphatase (AP). Highly reliable and discernible mixed labeling was achieved only after iniriallabeling with IGSS followed by AP labeling using the substrates naphthol AS-MX phosphate/Fast Blue or naphthol AS-HI phosphate/New Fuchsin, respectively. To ensure utmost specificity, we applied FlTC-conjugated mouse monoclonal antibodies and rabbit anti-FlTC immunoglobulins visualized by AP-labeled immunoglobulins and the respective substrate in a ...
We have developed a reliable and sensitive immunohistochemical staining technique which allows the simultaneous demonstration of two different antigens expressed in or on the same cell (referred to as mixed labeling), together with the evaluation of the general histopathological appearance of the tissue. The staining procedure combines a three-step (streptavidin-biotin) immunogold-silver staining (IGSS) with a three-step immunoenzymatic labeling. For this purpose, we investigated the compatibility ofIGSS with various substrates of peroxidase or alkaline phosphatase (AP). Highly reliable and discernible mixed labeling was achieved only after iniriallabeling with IGSS followed by AP labeling using the substrates naphthol AS-MX phosphate/Fast Blue or naphthol AS-HI phosphate/New Fuchsin, respectively. To ensure utmost specificity, we applied FlTC-conjugated mouse monoclonal antibodies and rabbit anti-FlTC immunoglobulins visualized by AP-labeled immunoglobulins and the respective substrate in a ...
Demonstrates neutral and acidic mucosubstances on tissue at pH 2.5 imparts a blue color to the acidic mucins and other carboxylated or weakly sulphated acid mucosubstances. Periodic Acid Schiff (PAS) reaction is then used to stain basement membranes, glycogen and neutral mucosubstances pink to red. Mixtures of neutral and acidic mucosubstances will appear purple due to positive reactions with both Alcian Blue and PAS.. Learn More ...
Demonstrates neutral and acidic mucosubstances on tissue at pH 2.5 imparts a blue color to the acidic mucins and other carboxylated or weakly sulphated acid mucosubstances. Periodic Acid Schiff (PAS) reaction is then used to stain basement membranes, glycogen and neutral mucosubstances pink to red. Mixtures of neutral and acidic mucosubstances will appear purple due to positive reactions with both Alcian Blue and PAS.. Learn More ...
Posterior gut (Alcian Blue-PAS) from fish fed (A) Fish oil-based diet (FO), (B) FO- and MOS-based diet (FOMOS), (C) soy bean meal-based diet (SBO), and (D) SBO-
Barretts esophagus (BE) is the main pathological precursor of esophageal adenocarcinoma (EAC). Progression to high-grade dysplasia (HGD) or EAC from nondysplastic BE (NDBE), low-grade dysplasia (LGD) and indefinite for dysplasia (IND) varies widely between population-based studies and specialized centers for many reasons, principally the rigor of the biopsy protocol and the accuracy of pathologic definition. In the Republic of Ireland, a multicenter prospective registry and bioresource (RIBBON) was established in 2011 involving six academic medical centers, and this paper represents the first report from this network. A detailed clinical, endoscopic and pathologic database registered 3,557 patients. BE was defined strictly by both endoscopic evidence of Barretts epithelium and the presence of specialized intestinal metaplasia (SIM). A prospective web-based database was used to gather information with initial and follow-up data abstracted by a data manager at each site. A total of 2,244 ...
Checkpoint inhibitors, including nivolumab and pembrolizumab, are novel therapies that have demonstrated significant efficacy in the management of metastatic malignancy.10 The immunological adverse event profile related to these therapies is broad, with rates of enterocolitis as high as 30%.7,11 The median time of onset of symptoms is 6-7 weeks from initiation of therapy but can vary, with some reports as late as 6 months, as in our case.11 The colitis is manifested by diarrhea, defined as greater than 4 stools per day, with blood or mucous, abdominal discomfort or cramping, and radiographic or endoscopic evidence of inflammation.12 The pathophysiology is characterized by lymphocytic infiltration in the lamina propria and epithelium of the involved mucosa. This inflammation can be transmural, and spontaneous bowel perforations have been reported.13 Interestingly, the remainder of the GI tract is rarely involved. Management includes exclusion of other infectious etiologies followed by initiation ...
Histology: the tumour has an expansive growing pattern mainly surrounded by fibrous tissue and it is composed by solid and glandular/pseudoglandular components. The cells are small, myoepithelial and epithelial cells, polarized around two types of spaces - glandular or pseudolumina. The true glandular spaces are surrounded by luminal cells and contain neutral mucin (PAS positive); the pseudolumina are stromal spaces filled by cylinders of hyaline material, surrounded by basal/myoepithelial cells; these spaces stain for Alcian Blue and collagen IV. The cells in the solid areas and around the cylinders stain for p63, smooth-muscle actin as well as for high-molecular-weight keratins. Cells around the lumina stain for low-molecular-weight keratin (for example keratin 7), as well as for CD117. On the cystic component, squamous metaplasia can be observed. Focally mainly in the solid areas the cells are bigger with evident nucleoli and the mitotic index is higher. Necrotic foci can be observed ...
The Alcian Blue (pH 2.5) Stain Kit is intended for use in the histological visualization of sulfated and carboxylated acid mucopolysaccharides and sulfated and carboxylated sialomucins (glycoproteins ...
산약 (Dioscoreae Rhizoma)의 수용성 점액물질(mucilage)은 Polysaccharide와 Protein으로 구성되어 있는 proteoglycan으로 알려져 왔다. 본 연구에서는 종래의 mucilage 정제법인 에탄올 침전법과는 다른 새로운 방법으로 mucilage 정제를 실시하고자 하였으며, 산약의 mucilage에 대한 정량법을 확립하고, 산약의 가공에 따른 mucilage 함량변화를 알아보았다. 시료산약은 재배품종으로서 시판되고 있는 두 가지 형태의 마 (곤봉형 마, 杖형 마)를 사용하였고, 각각의 생품과 증건품을 가지고 실험하였다. 산약의 물 추출액을 투석한 추, Bio-gel P-100과 Sephadex G-150을 사용하여 겔 여과한 결과, polysaccharide 부분과 Protein 부분이 서로 분리되어 나타나는 것으로 보아, 산약의 mucilage 에는 Protein이 함유되어 있지 않음을 확인할 수 있었다. 한편, mucilage 정량 방법으로는 산약의 mucilage성분이 alcian blue 와 ...
Last year my friend Diamond and I took a roadtrip up to Philadelphia to scout out the new crop of underground Shoegazer bands at the 1st Annual Pop-Noise Festival. So underground in fact, that over the course of the 10 bands or so, Diamond and I realized that we were the only people there other than band members and their parents watching the show. Id imagine that it was a tad embarrassing for the bands to have an audience of just two people, however that didnt stop them from rocking out shoegazer style for about 12 hours straight.. Without a doubt, the best new shoegazer band we found that day was a band from New York called A Place To Bury Strangers (with the exception of Alcian Blue who we had to skip to drive back to DC). These guys rocked like nothing Diamond or I had ever seen. Ever since then we have been waiting for them to come to DC to play. In fact I even toyed with the idea of promoting a show with them on my birthday just to get to see them again.. Fortunately I wont have to go ...
Internal links within this website are funded and maintained by the Beef Checkoff. All other outgoing links are to websites maintained by third parties. Read more ,,, ...
Cocktail of periodic Acid Schiff and papanicolaou: Novel staining technique for the identification of leukemic eosinophils A pilot study ...
This FlowCellectMitoDamage Kit for Flow Cytometry includes MitoSense Red, a fluorescent cationic dye that accumulates in the mitochondria & is responsive to mitochondrial potential changes. Find MSDS or SDS, a COA, data sheets and more information.
Notice that R8C1 and R8C3 are green and red, in some order. In particular, R8C3 is one of 2, 3, or 4. Now, suppose that R6C3 is a 6. This would force R8C3 to be either a 2 or a 3, since there are two numbers in between the ends of that thermometer - hence, it is red. This means R8C1 is green; in fact, R8C1 would be a 3, since R5C2 has to be a 3 by our ratio condition. Thus, the numbers in between R8C3 and 6 on that thermometer have to be 4 and 5. However, we cannot use a 5 here as weve already placed one in each row, column, and box. This gives us a contradiction, and since the only assumption we made here was that R6C3 was a 6, we conclude that R6C3 has to be an 8 instead. This means that R6C2 is a 4, making all blue cells 8 and all green cells 4: ...
Just to clarify for anyone that is unfamiliar, phagocytosis is the process in which one cell engulfs another. In this case, the big, blue cell is about to swallow the little, purple cell ... And the purple cell is thinking: Oh, no ...
Various miRNAs have been reported to regulate the chondrogenic differentiation of bone marrow mesenchymal stem cells (BMSCs); however, whether miR-134 plays a role in this biological process remains undetermined. In the present study, we first evaluated the chondrogenic differentiation of BMSCs by Alcian blue staining, and examined the miR-134 expression by quantitative real-time PCR (qRT-PCR) during this process. And miR-134 inhibitor was used to investigate the functions of miR-134 in chondrogenic differentiation of BMSCs by Alcian blue staining, qRT-PCR, and Western blot. Subsequently, the correlation between miR-134 and SMAD6 was assessed via bioinformatics analysis and dual-luciferase reporter assay. Finally, the role of SMAD6 in chondrogenic differentiation of BMSCs was also determined through Alcian blue staining, qRT-PCR, and Western blot. As results showed that miR-134 expression was significantly down-regulated during chondrogenic differentiation, and inhibition of miR-134 obviously ...
The cationic dye, chrysoidine, has been used for the first time as a probe for the indirect photometric detection of cations. The dye has been used as a probe at concentrations of 5 mM, which is roughly an order of magnitude higher than for other cationic dyes used previously for the same purpose, in order to minimise electromigrational dispersion. Baseline instability was minimised by a combination of coating the capillary with poly(ethyleneimine), addition of a neutral polymer to the electrolyte, and the application of a small amount (20 mbar) of hydrodynamic pressure during the separation. Separation of a mixture containing alkali metals, alkaline earths, transition metals and lanthanides was achieved by the addition of 2-hydroxyisobutyric and lactic acid as complexing agents. Excellent peak shapes were observed over a wide range of analyte mobilities due to the moderate mobility of the probe. The high absorptivity (26 733 l mol−1 cm−1) provided by chrysoidine in comparison with typically ...
Beck, Daniel, Vasisht, Nishi, Baglieri, Jacopo, Monteferrante, Carmine G., van Dijl, Jan Maarten, Robinson, Colin, Smith, Corinne J. (2013) Ultrastructural characterisation of Bacillus subtilis TatA complexes suggests they are too small to form homooligomeric translocation pores. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1833 (8). pp. 1811-1819. ISSN 0167-4889. (doi:10.1016/j.bbamcr.2013.03.028) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:34574) ...
Pig duodenum stained to differentiate carboxylated mucus in Brunners glands (yellow) from sulfonated mucus in gobletcells (blue-green). Alcian blue pH 1.0 + periodic acid + sodium metabisulfite + Alcian yellow = Brazilliant ...
Tujuan penelitian ini adalah mempelajari morfologi kelenjar parotis dan kelenjar mandibularis landak jawa secara makro dan mikro. Sampel didapatkan dengan melakukan preparir di daerah kepala, selanjutnya jaringan dibuat preparat histologi menggunakan metode paraffin. Slide jaringan kemudian dilakukan pewarnaan histokimia Hematoksilin eosin (HE), alcian blue (AB) pH 2.5, periodic acid Schiff (PAS), dan Casson trichrome. Hasil dari penelitian menunjukkan kelenjar parotis landak jawa memiliki tekstur lunak, berlobus, dan berukuran lebih besar dari kelenjar mandibularis. Kelenjar mandibularis memiliki tekstur kenyal dan tidak berlobus. Secara mikroskopis kelenjar parotis merupakan kelenjar serous murni, sedangkan kelenjar mandibularis merupakan kelenjar campuran. Asinar sereous pada kelenjar parotis tidak mengandung karbohidrat netral dan asam. Asinar mukous pada kelenjar mandibularis mengandung karbohidrat asam dan netral dengan intensitas kuat (+++). Asinar serous pada kelenjar mandibularis ...
Patients should have had a liver biopsy showing at least Stage 3 disease prior to being considered for this study. A baseline liver biopsy is necessary for inclusion in the study. Baseline liver biopsies can be performed within six months of entering the study.. In patients with cirrhosis and endoscopic evidence of portal hypertension, a biopsy within the last 2 years is acceptable as the baseline biopsy. For patients with established cirrhosis on liver biopsy and no portal hypertension, a biopsy within 12 months can be used as the baseline biopsy if it is available for evaluation by the Pathology core. All these patients will still require liver biopsy at 2 years and 4 years. The decision to biopsy at 2 and 4 years is also a clinical decision and in the presence of clinical progression or coagulopathy, or where there may be a risk from liver biopsy, the Investigator should call the PI, Dr. Afdhal for a waiver of biopsy. Patients with Ishak Stage 3 and 4 require a biopsy within 6 months of ...
In the gastric mucosa of a patient with Helicobacter pylori infection and a precancerous lesion called incomplete intestinal metaplasia (Genta stain), the gastric cells lining a gland have been replaced by absorptive intestinal cells with visible brush border and goblet cells, which are normally present in the intestine, but not in the stomach. Goblet cells contain acidic mucins stained blue by Alcian blue. This type of precancerous transformation is caused by long-term infection with H. pylori, visible in the lumen of the gland as curved bacterial rods stained black by silver stain. A few bacteria invade the goblet cells and can be found within blood capillaries, where they bind to red blood cells (see Aspholm et al. 2006).. ...
The fixation of dissociated chick embryonic cells in the presence of ruthenium red or alcian blue revealed a remarkable reduction in the binding of these cations to cell surfaces following tryptic digestion at 37 °C, but not after trypsin used at 4 °C or EDTA.. The reconstitution of anionic sites present over the cell plasma membrane was studied in retina, liver and heart cells from embryos of different ages, cultivated according to a method which promotes cell reaggregation. All these cells regenerated ruthenium red-stainability within the first hour of culture. Culturing at 15 °C, substitution of saline for the culture medium or addition of puromycin or of neuraminidase to the culture media did not affect this restitutive process. Neuraminidase and hyaluronidase digestion were not able to alter the ruthenium red-binding, once it was present.. From these data and from the reports available in the literature it has been concluded that the anionic binding-sites cannot be identified with the ...
* found in: Methyl Orange, Trypan Blue Solution 0.4%, Xylene Cyanol FF, Neutral Red, Safranin O, Alcian Blue 8 GX, Nitroblue Tetrazolium Chloride, Congo..
Multipotent mesenchymal stem cells (MSCs) are present in several tissue and may proliferate extensively differentiation of MSCs to mesodermal lineage For adipogenic differentiation, MSCs were seeded in 24 very well china at a focus of 2104 and cultured in adipogenesis differentiation moderate (Lifestyle Technology). S AZ 3146 i9000 yellowing was performed to identify gathered calcium supplement. For chondrogenic difference, a total of 8104 MSCs in 20 m of lifestyle moderate had been plated in the middle of 24 well china. After 3 hours incubation, chondrogenesis difference moderate was supplied (Lifestyle Technology). After 14 or 21 times of lifestyle, cells had been set in 4% paraformaldehyde. After that, Alcian Blue Yellowing was performed, which discovered sulfated proteoglycan wealthy matrix. Change transcription-quantitative PCR (RT-qPCR) and Traditional western mark evaluation Total RNA was ready by treatment with Trizol Reagent (Sigma-Aldrich, St. Louis, MO) and genomic DNA was taken out ...
Supplementary MaterialsS1 Fig: Manifestation of stem cell markers in hITPCs. days. The cultures (n = 3) were subjected to Alcian blue staining or qPCR to examine gene expression.(TIF) pone.0213912.s003.tif (538K) GUID:?83209185-4D29-4BAE-A6C6-9EDE10D7D8E7 Data Availability StatementAll relevant data are within the manuscript and its Supporting Information files. Abstract Glucose metabolism is altered in injured and healing tendons. However, the mechanism by which the glucose metabolism is involved in the pathogenesis of tendon healing process remains unclear. Injured tendons do not completely heal, and induce fibrous scar tissue and chondroid lesion often. Because previous research show that tendon progenitors play jobs in tendon restoration, we asked whether connective cells progenitors showing up in wounded tendons alter blood sugar rate of metabolism during tendon healing up process. We isolated connective cells progenitors through the human hurt tendons, acquired at the proper period of ...
A A beta 42 abl acid phosphatase ACSM1 actin-general actin-alpha cardiac actin-alpha smooth muscle actin-muscle specific AE1/AE3 (cytokeratin) AF4 AGR2 AKT1 albumin alcian blue ALK alkaline phosphatase ALL1 alpha-1-antichymotrypsin alpha-1-antitrypsin alpha-fetoprotein alpha-lactalbumin AMACR AML1 androgen receptor APC API2-MALT1 Apolipoprotein D arginase1 ATM ...
Fingerprint Dive into the research topics of Ultrastructural characterization of maize (Zea mays L.) kernels exposed to high temperature during endosperm cell division. Together they form a unique fingerprint. ...
The lining of the intestinal tract in cattle secretes a protective layer of mucous that contains high concentrations of proteins and carbohydrates. This mucus layer protects the lining of the gut from physical abrasion, but also serves as an important food source for colonization of the gut by bacteria. There is evidence that the composition of intestinal mucus may directly influence makeup of the bacterial community within the gut, indicating a selective advantage for bacteria capable of utilizing mucus components. Pathogenic organisms, including E. coli O157:H7, are somewhat unique in terms of their ability to utilize some components of mucus, giving them a selective advantage for growth. Additionally, there is evidence to suggest some bacterial species can further sustain their growth advantage by directly influencing the composition of mucus secretions. It also has been well established that certain species of microorganisms also can produce compounds which stimulate mucus release by the ...
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PAS (Periodic Acid Shiff) stain. PAS (Periodic Acid Shiff) stain is the most versatile and widely used technique for carbohydrate visualization. From a diagnostic point of view...
Abstract Submission Template.Do not type in royal blue cells.1. Enter title (ALL CAPS), authors, affiliation, address, and e-mail of corresponding author in turquoise cell. Overwrite present contents.2. Enter abstract text in yellow cell. Overwrite present contents
Alcian blue stain. The fibrosis is either evenly distributed between myocytes or follows anatomic structures such as blood ...
Alcian blue stain. Subepicardial fibrosis (epicardium at top) Myocardial infarction management Myocardial infarction ...
Alcian blue stain. Non-keratinized stratified squamous epithelium, image highlights the epithelial nucleuses, rest of the ...
Alcian blue stain. Replacement fibrosis in myocardial infarction, being boundless and dense. Wynn, Thomas A. (2004). "Fibrotic ...
"Alcian Blue - Alcian Blue , Songs, Reviews, Credits , AllMusic". AllMusic. Retrieved 2018-03-14. Monger, James. "Animal ... Ward, Brian (1998). Just My Soul Responding: Rhythm and Blues, Black Consciousness, and Race. University of California Press. ...
The myxoid background stroma stain positive for Alcian blue stain. Many mast cells can be seen dispersed in the stroma. ...
Early lesions, rich in acid mucopolysaccharides, stain positively with alcian blue. When medullary thyroid cancer is present, ... Luxol fast blue staining identifies myelin sheathing of some fibers, and lesional cells react immunohistochemically for S-100 ...
Alcian blue will give a blue staining to the stromal matrix material. Mucicarmine will also highlight the stromal matrix (pink ...
Dingerkus, Guido; Uhler, Lowell D. (1977-01-01). "Enzyme Clearing of Alcian Blue Stained Whole Small Vertebrates for ... usually alizarin red and alcian blue. Diaphonization was first developed in 1977 by Guido Dingerkus and L.D. Uhler. In 2017, ...
Contributors to the band also include Matt Welch of the band Alcian Blue. Tyler now also plays in the band Alias Linn. Wood and ... The first lineup (2000-2002) featured Bryan Smith on bass, and Sam Chintha of the band Alcian Blue drumming. Adam Robinson (who ...
Mucin is observable after the application of "alcian blue dye" on the tissue specimen. The histological observation of goblet ...
... alcian blue, Verhoeff hematoxylin, crocein scarlet combined with acidic fuchsine and saffron. At pH 2.5, alcian blue is fixed ... Movat's stain showing thickening of the spongiosa layer (blue) in myxomatous degeneration of the aortic valve. Movat's stain ...
Micrograph of Barrett's esophagus (left of image) and normal stratified squamous epithelium (right of image), Alcian blue stain ... High-magnification micrograph of Barrett's esophagus showing the characteristic goblet cells, Alcian blue stain ... Use of the histochemical stain Alcian blue pH 2.5 is also frequently used to distinguish true intestinal-type mucins from their ...
Alcian blue/periodic acid-Schiff (AB/PAS or AB-PAS) uses alcian blue before the PAS step. PAS staining is mainly used for ... dyes Methyl blue Methyl violet Methylene blue New methylene blue Potassium ferricyanide Potassium ferrocyanide Prussian blue ... Carboxyfluorescein DyLight Fluor, a product line of fluorescent dyes Egyptian blue Eosin Erythrosine Fluorescein Fluorescein ...
Whipple's disease: Alcian blue with apparently eosin counterstain enlarged villus with many macrophages. ...
The histochemical stain Alcian blue pH 2.5 is also frequently used to distinguish true intestinal-type mucins from their ...
The use of Alcian blue to stain these otherwise transparent molecules has been beneficial in more efficiently analyzing them ...
Micromass cultures of C3H-10T1/2 cells at varied oxygen tensions stained with Alcian blue. ...
"Alcian Blue - Artist Biography". AllMusic. All Media Network. Retrieved 9 January 2015. Monger, James Christopher. "The Amazing ... Action Steve Adey Adorable The Afghan Whigs The Ailerons Air Miami The Airborne Toxic Event Airhead Alabama Shakes Alcian Blue ... Lizard Jet Jets to Brazil Jimmy Eat World JJ72 Jjamz Joan of Arc Johnny Foreigner Johnossi Daniel Johnston Jon Spencer Blues ... Party Young the Giant Young Knives Young Rival The Young Veins Youngblood Hawke Youth Group Yuck Yukon Blonde Yves Klein Blue ...
Other dyes used in connection with Van Gieson staining include: Alcian blue Amido black 10B Verhoeff's stain Jocelyn H. Bruce- ...
... alcian blue MeSH D03.438.473.133 - captan MeSH D03.438.473.155 - carbolines MeSH D03.438.473.193 - chlorisondamine MeSH D03.438 ... methylene blue MeSH D03.494.741.533 - moricizine MeSH D03.494.741.550 - nonachlazine MeSH D03.494.741.575 - perazine MeSH ...
When vessels are stained with cationic dyes such as Alcian blue stain, transmission electron microscopy shows a small, ...
Adorable Air Formation Airiel Alcest Alcian Blue Alison's Halo Alvvays Amesoeurs Ammonia Wash Apollo Heights Asobi Seksu ...
... originally defined by their ability to be stained visible by acidic Alcian Blue. Their free-floating characteristic sets TEPs ...
... shades of blue Alcian blue stain Leung, Jennifer K.; Gibbon, Kevin J.; Vartanian, Robert K. (1996-06-01). "Rapid Staining ... Alcian yellow is used in histology in conjunction with toluidine blue to help visualize H. pylori. The resulting structures are ... Alcian yellow is an azo dye with the formula (CH3[(CH3N)2CS]2C6H2SNCC6H4)2N2. Its structure consists of a largely conjugated ... stained as follows: - Mucin: yellow - H. pylori: blue - Other cellular components/background: ...
... of which the Alcian blue 8G (also called Ingrain blue 1, and C.I. 74240, formerly called Alcian blue 8GX from the name of a ... Solid Alcian blue is a combustible powder and should never be handled close to heat or a naked flame. Heating Alcian blue ... "Alcian Blue". The I.C.I. Journal: 59-60. March 1948. GB 586340 GB 587636 Steedman H. F. (1950). "Alcian blue 8GS: a new stain ... In addition to its use as a dye or stain Alcian blue also finds other material science uses. Alcian blue has been used as an ...
"Alcian Blue - Alcian Blue - Songs, Reviews, Credits - AllMusic". AllMusic. Retrieved 2016-08-09. "Safranin Sound". ... Alcian Blue was an American shoegaze and new wave-influenced band operating out of the Washington, D.C. suburb of Takoma Park ... Slow Colorless Stare CD (2001, Safranin Sound) Alcian Blue CD (2006, Elephant Stone) Angelica Take Me Down EP (2003, Safranin ...
Alcian blue stain. *Movat's stain. *Jaswant Singh-Bhattacharji (JSB) stain. Tissue stainability. *Acidophilic ...
Alcian blue stain. *Cyanine. *Jaswant Singh-Bhattacharji (JSB) stain. *H&E stain *Haematoxylin ... Methylene blue/malachite green. Red. Blue Studies have shown that an AFB stain without a culture has a poor negative predictive ... When counter stain is applied, non-acid-fast bacteria pick it up and become blue (methylene blue) or green (malachite green) ... The reagents used for Ziehl-Neelsen staining are - carbol fuchsin, acid alcohol, and methylene blue. Acid-fast bacilli are ...
Nile blue[edit]. Nile blue (or Nile blue A) stains nuclei blue. It may be used with living cells. ... Coomassie blue[edit]. Coomassie blue (also brilliant blue) nonspecifically stains proteins a strong blue colour. It is often ... Methylene blue[edit]. Methylene blue is used to stain animal cells, such as human cheek cells, to make their nuclei more ... Nile red (also known as Nile blue oxazone) is formed by boiling Nile blue with sulfuric acid. This produces a mix of Nile red ...
"Alcian Blue - Alcian Blue - Songs, Reviews, Credits - AllMusic". AllMusic. Retrieved 2016-08-09. "Safranin Sound". ... Alcian Blue was an American shoegaze and new wave-influenced band operating out of the Washington, D.C. suburb of Takoma Park ... Slow Colorless Stare CD (2001, Safranin Sound) Alcian Blue CD (2006, Elephant Stone) Angelica Take Me Down EP (2003, Safranin ...
... of which the Alcian blue 8G (also called Ingrain blue 1, and C.I. 74240, formerly called Alcian blue 8GX from the name of a ... Solid Alcian blue is a combustible powder and should never be handled close to heat or a naked flame. Heating Alcian blue ... "Alcian Blue". The I.C.I. Journal: 59-60. March 1948. GB 586340 GB 587636 Steedman H. F. (1950). "Alcian blue 8GS: a new stain ... In addition to its use as a dye or stain Alcian blue also finds other material science uses. Alcian blue has been used as an ...
... alcian blue include Using Unfixed, Frozen Tissues to Study Natural Mucin Distribution, Adult Zebrafish Injury Models to ... Alcian Blue: A copper-containing dye used as a gelling agent for lubricants, for staining of bacteria and for the dyeing of ...
After injection of Alcian blue at LSP (lumbar sacral point) of the GV meridian, we observed a blue line at the GV meridian ... Acupuncture meridian Alcian blue Bonghan duct system Lumbar sacral point This is a preview of subscription content, log in to ... 2007) Alcian Blue Staining Technique for Tracing the Governing Vessel (GV) Meridian in Rat. In: Magjarevic R., Nagel J.H. (eds ... Only very recently we found a staining dye, Alcian blue that stains the acupuncture meridian and were able to trace the ...
Alcian Blue-tetrakis(methylpyridinium) chloride; CAS Number: 123439-83-8; Synonym: A,B,C,D-tetrakis(Pyridiniomethyl) Cu(II) ... Alcian Blue-. tetrakis(methylpyridinium) chloride Synonym: A,B,C,D-. tetrakis(Pyridiniomethyl) Cu(II) phthalocyanine chloride ... This dye is similar in structure and properties to Alcian Blue 8GX, with pyridiniomethyl substituents in each ring instead of ...
Alcian Blue in conjunction with PAS (Mowry, 1956) is commonly used to differentiate acid and neutral mucopolysaccharides. It ...
Stain strongly sulfated mucins with Alcian Blue Stain Kit ab150661. Abcam offers , 1,000 assay kits cited in , 3,500 ... ab150661 (Alcian Blue pH 1.0) staining strongly sulphated mucins (blue) in formalin fixed paraffin embedded human colon, ... incubate in Alcian Blue pH 1.0 for 30 min. - rinse in water. - stain in nuclear fast red for 5 min. - rinse in water. - ... Alcian Blue staining protocol summary:. - deparaffinize sections if necessary and hydrate. - incubate in acetic acid for 3 min ...
Ingrain Blue 1. SMILES. CN(C)C(=[N+](C)C)SCC1=CC2=C(C=C1)C3=NC4=C5C=C(C=CC5=C([N-]4)N=C6C7=C(C=CC(=C7)CSC(=[N+](C)C)N(C)C)C(=N6 ...
Alcian Blue Staining Protocol. Prepared by. ROY ELLIS. IMVS Division of Pathology. The Queen Elizabeth Hospital. Woodville Road ... 4. Steedman, H.F. 1950 Alcian blue 8GS; A new stain for mucin. Quarterly Journal of Microscopic Science, Vol 91, p477-479. ... Dissolve 1 gm alcian blue in 100mls of 10% sulphuric acid.. (Sulphuric acid 10% Distilled water .......................... 90ml ... By matching the ionic strength and/or the pH of the solvent, solutions of the copper phthalocyanins (eg Alcian blue) are used ...
Alcian BlueIBA 05/2013 - 07/2012. 2. Vascular Endothelial Growth Factor A (Vascular Endothelial Growth Factor)IBA 03/2012 - 12/ ... Alcian Blue. 5/2013. Immunohistochemical investigations of orbital infantile hemangiomas and adult encapsulated cavernous ...
... From:. Anita Jennings ,[email protected],. Thanks to all who responded to the Alcian blue ...
... From:. Geoff McAuliffe Hi Tom: Alcian Blue will stain the glycosaminoglycans in the ... Still, the traditional method is 1% Alcian Blue in 3% acetic acid for a pH of 2.5. At 2.5 both sulfated and carboxylated GAGs ... with alcian blue. Interest in cellular morphology of nucleus pulposus, transition and annulus cells. > >What pH of the stain ... You might try 0.05% Toluidine blue in dist water with a pH of about 4.0 for 10-15 minutes, dist water rinse and dehydration in ...
Demonstrate the presence of both neutral and acidic mucosubstances with the Thermo Scientific Richard-Allan Scientific Alcian ... Includes: Alcian Blue pH 2.5 stain solution, periodic acid solution (0.5%) and Schiff reagent ... Can be used alone to stain acid mucins and other carboxylated and sulfated acid mucosubstances blue on tissue sections ...
... Teratology. 1980 Dec;22(3 ...
Leica Alcian Blue Special Stain Kit Consumables Leica Alcian Blue Special Stain Kit *Product ... Alcian Blue is normally prepared at an acidic pH of 2.5 and is used to identify acid mucopolysaccharides and acetic mucins. ...
Orcein-alcian blue is a safer, cheaper, and quicker method than high iron diamine-alcian blue which can be safely introduced ... Orcein-alcian blue staining, a new method for the simultaneous demonstration of sulphated and sialomucins in gastrointestinal ... Orcein-alcian blue staining: a new technique for demonstrating acid mucins in gastrointestinal epithelium. ... Orcein-alcian blue staining: a new technique for demonstrating acid mucins in gastrointestinal epithelium. ...
Our lab has used the Alcian blue - Alizarin red staining method with certain modifications to characterize skeleton deformities ... staining the cartilage is done with 0.02% alcian blue (as in your protocol) or 0.2% alcian blue (as in MBC paper)?. Thanks ... Alcian blue 8GX (prepared in ethanol/ glacial acetic acid, 7:3) for 1 to 2 days.. Note: Cartilage tissues will be stained blue ... Zhang, P. (2012). Alcian Blue - Alizarin Red Staining of Mouse Skeleton. Bio-protocol 2(8): e162. DOI: 10.21769/BioProtoc.162. ...
Alcian blue explanation free. What is Alcian blue? Meaning of Alcian blue medical term. What does Alcian blue mean? ... Looking for online definition of Alcian blue in the Medical Dictionary? ... Alcian blue , definition of Alcian blue by Medical dictionary https://medical-dictionary.thefreedictionary.com/Alcian+blue ... Alcian blue. Also found in: Acronyms, Encyclopedia, Wikipedia. Al·ci·an blue. (alsē-an blū), [C.I. 74240] A complex ...
Looking for abbreviations of ABRBC? It is Alcian Blue on Red Blood Cell Membrane. Alcian Blue on Red Blood Cell Membrane listed ... Alcian Blue on Red Blood Cell Membrane - How is Alcian Blue on Red Blood Cell Membrane abbreviated? https://acronyms. ... a href=https://acronyms.thefreedictionary.com/Alcian+Blue+on+Red+Blood+Cell+Membrane,ABRBC,/a,. *Facebook ... redirected from Alcian Blue on Red Blood Cell Membrane) Acronym. Definition. ABRBC. Alcian Blue on Red Blood Cell Membrane. ...
Alcian yellow-toluidine blue Leung stain explanation free. What is Alcian yellow-toluidine blue Leung stain? Meaning of Alcian ... What does Alcian yellow-toluidine blue Leung stain mean? ... Looking for online definition of Alcian yellow-toluidine blue ... Alcian yellow-toluidine blue (Leung) stain. (redirected from Alcian yellow-toluidine blue Leung stain) Alcian yellow-toluidine ... Alcian yellow-toluidine blue Leung stain , definition of Alcian yellow-toluidine blue Leung stain by Medical dictionary https ...
... Hobbs, Carl carl.hobbs at kcl.ac.uk Thu Aug 23 13:32:13 CDT ... Perhaps Alcian blue at pH3 ( all acidic mucins) on one slide/section and Ab pH1 ( highly sulphated mucins only) on a sequential ... Previous message (by thread): [Histonet] Alcian Blue digested w Hyaluronidase - any alternatives? ... Next message (by thread): [Histonet] Alcian Blue digested w Hyaluronidase - any alternatives? ...
Find the best Labs and Diagnostic Centers for Alcian Blue Stain Urine Online in Bhubaneswar. View its pre-requirement, prices ...
The Alcian blue is used on tissue samples taken from the gastrointestinal tract and can be used to detect processes such as ... Alcian Blue Stain. View cart "Peripheral Blood Smear" has been added to your cart. ...
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Alcian Blue Pathology Outlines. Posted byadmin April 27, 2019. Thomas Edison Film Projector Each film was approximately 17 ... Alcian blue stain showed pools of mucin in the.. From the Departments of *Rheumatology and Clinical Immunology and †Trauma and ... Then we made thinly sliced dorsal sections of removed trachea and stained them with hematoxylin and eosin (HE) or alcian blue ( ... Importantly, at the age of 6 months, around 40% of CPK mice displayed cystic lesions classified as alcian blue/PAS-positive ...
... and Alcian Blue methods to demonstrate the full complement of tissue proteoglycans. ... Alcian Blue-PAS Stain Kit utilizes the properties of both the Periodic Acid Solution (PAS) ... The Alcian Blue-PAS Stain Kit utilizes the properties of both the Periodic Acid Solution (PAS) and Alcian Blue methods to ... The rationale of the technique is that by first staining all the acidic mucins with Alcian Blue, those acidic mucins which are ...
Hito Alcian Blue pH-2.5 OptimStain™ Kit is formulated for the staining of acid mucins, sulfated / carboxylated acid ... Hito Alcian Blue pH-2.5 OptimStain™ Kit has been tested extensively on the tissues from several species of animals and it is a ... Hito Alcian Blue pH-2.5 OptimStain™ Kit has been tested extensively on the tissues from several species of animals and it is a ... Hito Alcian Blue pH-2.5 OptimStain™ Kit is designed in a ready-to-use format and offers high quality, rapid and reproducible ...
Alcian blue staining. Cells were fixed with 4% paraformaldehyde for 10 min, stained with 1% Alcian blue 8GX (Sigma-Aldrich), ... Brightfield images of Alcian blue samples (Fig. 5E and fig. S1A) were taken using the Olympus microscope IX71. Phase-contrast ... E) Alcian blue staining showing sulfated proteoglycans characteristic of cartilaginous ECM in the indicated WT and Ship2 ... as determined by Western blotting for collagen 2 and Alcian blue staining for sulfated proteoglycans (Fig. 5, D and E, and fig ...
Alcian Blue Binding Assays. The phosphomannan content was determined by measuring the binding of the cationic dye Alcian Blue ... Alcian Blue and found that none of the complemented transformants were capable of restoring wild-type levels of Alcian Blue ... Aliquots of 1 mL were pelleted, and cells suspended in 1 mL of 30 μg/mL Alcian Blue (in 0.02 M HCl) and incubated at room ... Complementation of this strain with MNN41 did not increase Alcian Blue binding, but complementation with either MNN42 or MNN47 ...
Exposure of Skeletal Organic Matrix and Alcian Blue Staining.. Alcian blue solution was prepared in acetic acid 3% (10 g/liter ... digitata adherent cell aggregates after HCl digestion and Alcian blue staining. The blue areas indicate the presence of ... Alcian blue is commonly used to detect mucopolysaccharides and glycoproteins (42) and was shown to stain sulfated ... Samples were then incubated with Alcian blue solution (pH 2.5) for 30 min, followed by washing with artificial seawater. ...
  • ab150661 (Alcian Blue pH 1.0) staining strongly sulphated mucins (blue) in formalin fixed paraffin embedded human colon, counterstained with safranin o (pink). (abcam.com)
  • By m atching the ionic strength and/or the pH of the solvent, solutions of the copper phthalocyanins (eg Alcian blue) are used to react with (and thus demonstrate), the ionisable moiety of acidic proteoglycans (mucins). (ihcworld.com)
  • Alcian Blue is normally prepared at an acidic pH of 2.5 and is used to identify acid mucopolysaccharides and acetic mucins . (leicabiosystems.com)
  • Orcein-alcian blue staining: a new technique for demonstrating acid mucins in gastrointestinal epithelium. (bmj.com)
  • Orcein-alcian blue is a safer, cheaper, and quicker method than high iron diamine-alcian blue which can be safely introduced into routine laboratories for the study of acid mucins in the gastrointestinal diseases. (bmj.com)
  • Perhaps Alcian blue at pH3 ( all acidic mucins) on one slide/section and Ab pH1 ( highly sulphated mucins only) on a sequential slide? (utsouthwestern.edu)
  • Mar 07, 2019 · Mucin stains: Alcian blue: Stains acid-simple non-sulfated and acid-simple mesenchymal mucins at pH 2.5, acid-complex sulfated mucins at pH 1.0 and acid-complex connective tissue mucins at. (profissionaldebermuda.com)
  • The rationale of the technique is that by first staining all the acidic mucins with Alcian Blue, those acidic mucins which are also PAS positive will be chemically blocked and will not react further during the technique. (vitrovivo.com)
  • Hito Alcian Blue pH-2.5 OptimStain™ Kit is formulated for the staining of acid mucins, sulfated / carboxylated acid mucopolysaccharides and sulfated / carboxylated sialomucins (glycoproteins). (hitobiotec.com)
  • Newcomer Supply Alcian Blue pH 2.5, Multi-Tissue Control Slides use a combination of tissue sources for the positive histochemical staining of acid epithelial mucins (sialomucin, sulfomucin) as well as stromal (mesenchymal) mucin. (newcomersupply.com)
  • Click the button below to add the Alcian Blue (Mucins) Control Slides Paraffin Sections, 5 slides/pack to your wish list. (ihcworld.com)
  • Demonstrates neutral and acidic mucosubstances on tissue at pH 2.5 imparts a blue color to the acidic mucins and other carboxylated or weakly sulphated acid mucosubstances. (polysciences.com)
  • Alcian blue [AB] is a cationic dye that stains mucins especially acidic mucins into varying shades of blue colour depending on the pH of the dye. (bvsalud.org)
  • With recent increase in GIT tumours especially epithelial tumours, Alcian Blue staining of mucins produced by these tumours at different pH more than conventional pH might give valuable information on the property of these tumours. (bvsalud.org)
  • Tissue sections were stained with Alcian blue (AB)/periodic acid-Schiff (PAS), for mucins MUC5B and MUC5AC, and for neutrophils and its chemoattractant interleukin (IL) 8. (bmj.com)
  • Only very recently we found a staining dye, Alcian blue that stains the acupuncture meridian and were able to trace the governing vessel (GV) of a rat using this dye. (springer.com)
  • The edematous stem villous stroma stains for Alcian blue , but is negative for smooth muscle actin, which shows positive staining of normal villous stroma. (thefreedictionary.com)
  • stains mucus yellow and the microorganism itself blue. (thefreedictionary.com)
  • The diagnosis is made definitively in adults and children only by the presence of goblet cells containing acid mucin that stains with Alcian blue at pH 2.5 (5,6,23) False-positive and -negative. (profissionaldebermuda.com)
  • Alcian blue stains what? (brainscape.com)
  • In addition to its wide use as a stain Alcian blue has also been used in other diverse applications e.g. gelling agent for lubricating fluids, modifiers for electrodes, charged coating agents etc. (wikipedia.org)
  • On special stain ( alcian blue ) there was some mucin deposition in areas of degenerated collagen. (thefreedictionary.com)
  • In attempt to answer what was the importance of discovering an alternative method of manufacturing this compound, a company (Anatech Ltd, USA) that remanufactured Alcian blue says: "Alcian blue is highly selective for the tissue substances (given the proper solution pH), and forms insoluble complexes that withstand harsh subsequent treatment (like PAS) without destaining. (wikipedia.org)
  • After injection of Alcian blue at LSP (lumbar sacral point) of the GV meridian, we observed a blue line at the GV meridian above loose connective tissue. (springer.com)
  • The Alcian blue is used on tissue samples taken from the gastrointestinal tract and can be used to detect processes such as Barrett's esophagus. (apollodiagnostics.in)
  • The Alcian Blue-PAS Stain Kit utilizes the properties of both the Periodic Acid Solution (PAS) and Alcian Blue methods to demonstrate the full complement of tissue proteoglycans. (vitrovivo.com)
  • The colon was analyzed for the presence of aberrant crypt foci (ACF) under a stereomicroscope.The tissue was stained with hematoxylin and eosin and PAS alcian blue. (scielo.br)
  • Silver staining combined with alcian blue and hematoxylin-eosin for the detection of Lawsonia intracellularis in swine proliferative enteropathy. (thefreedictionary.com)
  • Then we made thinly sliced dorsal sections of removed trachea and stained them with hematoxylin and eosin (HE) or alcian blue (AB) for light microscopic examination. (profissionaldebermuda.com)
  • The samples were then stained with different dyes under standard conditions for hematoxylin-eosin techniques, Masson's trichrome, orcein, and periodic acid-Schiff Alcian blue to a pH of 2.5. (profissionaldebermuda.com)
  • Our lab has used the Alcian blue - Alizarin red staining method with certain modifications to characterize skeleton deformities in mice lacking Pek/Perk, encoding a translational control eIF2alpha kinase. (bio-protocol.org)
  • Should limp and blue cartilage be readily observed at this point, proceed to stain the sample with Alizarin red (prepared in 0.5% KOH) O/N. (bio-protocol.org)
  • Depletion of intestinal goblet cells was observed in 10 cases of PCV2 IHC-stained sections of colon counterstained with Alcian blue and hematoxylin. (thefreedictionary.com)
  • Alcian blue (/ˈælʃən/) is any member of a family of polyvalent basic dyes, of which the Alcian blue 8G (also called Ingrain blue 1, and C.I. 74240, formerly called Alcian blue 8GX from the name of a batch of an ICI product) has been historically the most common and the most reliable member. (wikipedia.org)
  • intratracheal instillation of TNFα induced EGF-R in airway epithelial cells, and subsequent instillation of EGF-R ligands increased the number of goblet cells, Alcian blue-periodic acid-Schiff staining (reflecting mucous glycoconjugates), and MUC5AC gene expression, whereas TNFα, EGF, or TGFα alone was without effect. (pnas.org)
  • Alcian Blue stained goblet cells in mouse asthma model. (cedars-sinai.edu)
  • The Alcian Blue colorimetric technique was used to assay the sulfated glycosaminoglycan (sGAG) portion of decorin. (mdpi.com)
  • After centrifuging to remove the sediment, wAPS in the supernatant was precipitated with alcian blue, a cationic dye. (int-res.com)
  • Dissolve 1 gm of alcian blue in 90mls of distilled water and 10mls of 1N hydrochloric acid. (ihcworld.com)
  • The Alcian Blue (pH 1.0) (Mucin Stain) is intended for use in the histological visualization of strongly sulfated mucosubstances. (abcam.com)
  • Mixtures of neutral and acidic mucosubstances will appear purple due to positive reactions with both Alcian Blue and PAS. (polysciences.com)
  • The combination of PAS staining with Alcian blue allows the additional identification of acidic mucosubstances at pH 2.5. (merckmillipore.com)
  • When the ready-to-use Alcian blue solution is applied to specimens pre-stained with PAS, nuclei will appear blue, acidic mucosubstances will be light blue, and polysaccharides or neutral mucopolysaccharides will be stained purple. (merckmillipore.com)
  • If Alcian blue is used with Nuclear fast red solution, acidic mucosubstances can be identified without differentiation of carboxyl and sulfate groups. (merckmillipore.com)
  • To assess glycosaminoglycans (GAGs) present in the newly synthesized ECM, deparaffinized sections were stained with Alcian Blue and van Gieson. (profissionaldebermuda.com)
  • Use of alcian blue has historically been a popular staining method in histology especially for light microscopy in paraffin embedded sections and in semithin resin sections. (wikipedia.org)
  • Still, the traditional method is 1% Alcian Blue in 3% acetic acid for a pH of 2.5. (histosearch.com)
  • Stain the sample with 0.02% Alcian blue 8GX (prepared in ethanol/ glacial acetic acid, 7:3) for 1 to 2 days. (bio-protocol.org)
  • A brief dip in Solution A: Acetic Acid 3%, Aqueous from Step #3 can be added before water rinses to remove excess Alcian Blue Solution if needed. (newcomersupply.com)
  • Note: Cartilage tissues will be stained blue. (bio-protocol.org)
  • Next, we performed Alcian blue and Sirius red staining for cartilage proteoglycan collagen, respectively. (thefreedictionary.com)
  • Cell nuclei are stained blue, while other structures appear in various red tones. (merckmillipore.com)
  • The stomach contents were immersed immediately in 10 ml of 0.02% Alcian blue in 0.16 M sucrose/0.05 M sodium acetate, pH 5.8, and incubated for 24 h at 20 [degrees]C. (thefreedictionary.com)
  • Additionally, the stroma surrounding the poroma nests was myxomatous in some of the lesions showing mucin deposition which was highlighted by Alcian blue stain. (profissionaldebermuda.com)
  • Hi Tom: Alcian Blue will stain the glycosaminoglycans in the extracellular matrix, it won't show much cellular morphology. (histosearch.com)
  • Image analysis of Alcian blue- and safranin O-stained F-group specimens showed increased production of glycosaminoglycans (GAGs) and proteoglycans (PGs), respectively. (mdpi.com)
  • Mast cell degranulation morphometric staining was carried out by two dyes, toluidine blue and alcian blue. (calpaininhibitor.com)
  • Alcian Blue in conjunction with PAS (Mowry, 1956) is commonly used to differentiate acid and neutral mucopolysaccharides. (nationaldiagnostics.com)
  • eosinophilic cytoplasm containing periodic acid-Schiff-positive and alcian blue-negative neutral. (profissionaldebermuda.com)
  • Dissolve 1 gm alcian blue in 100mls of 10% sulphuric acid. (ihcworld.com)
  • You might try 0.05% Toluidine blue in dist water with a pH of about 4.0 for 10-15 minutes, dist water rinse and dehydration in 3 changes of actone followed by clearing in fresh xylene. (histosearch.com)
  • The purified mast mobile preparations contained .ninety eight% mast cells, as determined by staining with toluidine blue. (calpaininhibitor.com)
  • Orcein-alcian blue staining, a new method for the simultaneous demonstration of sulphated and sialomucins in gastrointestinal epithelium was compared with the standard high iron diamine-alcian blue technique. (bmj.com)
  • Alcian blue staining can be combined with H&E staining, PAS staining and van Gieson staining methods. (wikipedia.org)
  • To determine the effect of pH and pattern of Alcian blue staining on primary GIT epithelial tumours tumours. (bvsalud.org)
  • ab150661 Alcian Blue pH 1 Stain Kit (Mucin Stain) staining formalin-fixed-paraffin embedded human GI tract. (abcam.com)
  • Rustamadji P, Wibowo J, Murtani B, Magdalena C. Periodic acid-Schiff and alcian blue immunohistochemistry to detect mucin in mucinous breast carcinoma. (ui.ac.id)
  • The Monstral blue found to coat the inside of copper vessels used to process phthalic acid derivatives had led to the discovery of Phthalocyanine in 1907. (wikipedia.org)
  • The chondrogenic differentiation potential was examined using a pellet culture, with the pellet size monitored and the degree of alcian blue staining observed. (thefreedictionary.com)
  • In the present study, we first evaluated the chondrogenic differentiation of BMSCs by Alcian blue staining, and examined the miR-134 expression by quantitative real-time PCR (qRT-PCR) during this process. (portlandpress.com)
  • And miR-134 inhibitor was used to investigate the functions of miR-134 in chondrogenic differentiation of BMSCs by Alcian blue staining, qRT-PCR, and Western blot. (portlandpress.com)
  • Finally, the role of SMAD6 in chondrogenic differentiation of BMSCs was also determined through Alcian blue staining, qRT-PCR, and Western blot. (portlandpress.com)
  • Diastase pre-treatment may be used to eliminate the presence of glycogen, prior to staining in alcian blue. (vitrovivo.com)
  • Move slides directly into Solution B: Alcian Blue Stain 1%, pH 2.5 Aqueous. (newcomersupply.com)
  • Blood vessels in the periphery of the necrotic areas were occluded by amphophilic, amorphous material (emboli) (Figure 1D) that strongly stained with alcian blue and was interpreted as fibrocartilaginous emboli (Figure 1D inset). (thefreelibrary.com)
  • Alcian blue can be used to quantitate acidic glycans both in microspectrophotometric quantitation in solution or for staining glycoproteins in polyacrylamide gels or on western blots. (wikipedia.org)
  • Stain with Alcian blue solution for 15 mins. (vitrovivo.com)
  • Hito Alcian Blue pH-2.5 OptimStain™ Kit has been tested extensively on the tissues from several species of animals and it is a simple solution for your research. (hitobiotec.com)
  • After injecting AB solution into the femoral vein of a mouse, the threadlike structures, stained deep blue, inside the inferior vena cava. (edu.hk)
  • The remaining alcian blue in solution was determined spectrophotometrically and was inversely proportional to the concentration of wAPS in the sediment. (int-res.com)
  • Dimethyl methylene blue (DMB or DMMB) method. (wikipedia.org)
  • Sure, instead use Alcian blue CEC method? (utsouthwestern.edu)
  • The Alcian Blue method was applied and the experimental coefficient of erythrocyte anion charge (expCEAC) was determined. (scielo.org.ar)
  • Mobile viability was checked utilizing the trypan blue exclusion method and cells had been usually .98% feasible [sixteen]. (calpaininhibitor.com)
  • An in vivo method using Alcian blue (AB) was developed for visualizing floating threadlike tissues inside blood vessels of mice. (edu.hk)
  • The capsule and fungus cell exhibited marked Alcian Blue and periodic acid-Schiff staining, respectively. (scielo.br)
  • Prussian Blue or Perls' reaction is used to demonstrate ferric iron and ferritin. (polysciences.com)