Alcian Blue: A copper-containing dye used as a gelling agent for lubricants, for staining of bacteria and for the dyeing of histiocytes and fibroblasts in vivo.Periodic Acid-Schiff Reaction: A histochemical technique for staining carbohydrates. It is based on PERIODIC ACID oxidation of a substance containing adjacent hydroxyl groups. The resulting aldehydes react with Schiff reagent to form a colored product.Glycosaminoglycans: Heteropolysaccharides which contain an N-acetylated hexosamine in a characteristic repeating disaccharide unit. The repeating structure of each disaccharide involves alternate 1,4- and 1,3-linkages consisting of either N-acetylglucosamine or N-acetylgalactosamine.Staining and Labeling: The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.Coloring Agents: Chemicals and substances that impart color including soluble dyes and insoluble pigments. They are used in INKS; PAINTS; and as INDICATORS AND REAGENTS.Histocytochemistry: Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.Tolonium Chloride: A phenothiazine that has been used as a hemostatic, a biological stain, and a dye for wool and silk. Tolonium chloride has also been used as a diagnostic aid for oral and gastric neoplasms and in the identification of the parathyroid gland in thyroid surgery.Sialomucins: A subcategory of mucins that contain SIALIC ACID.Metaplasia: A condition in which there is a change of one adult cell type to another similar adult cell type.Mucins: High molecular weight mucoproteins that protect the surface of EPITHELIAL CELLS by providing a barrier to particulate matter and microorganisms. Membrane-anchored mucins may have additional roles concerned with protein interactions at the cell surface.Mucus: The viscous secretion of mucous membranes. It contains mucin, white blood cells, water, inorganic salts, and exfoliated cells.Chondrogenesis: The formation of cartilage. This process is directed by CHONDROCYTES which continually divide and lay down matrix during development. It is sometimes a precursor to OSTEOGENESIS.Pyronine: Xanthene dye used as a bacterial and biological stain. Synonyms: Pyronin; Pyronine G; Pyronine Y. Use also for Pyronine B. which is diethyl-rather than dimethylamino-.Uronic Acids: Acids derived from monosaccharides by the oxidation of the terminal (-CH2OH) group farthest removed from the carbonyl group to a (-COOH) group. (From Stedmans, 26th ed)Cartilage: A non-vascular form of connective tissue composed of CHONDROCYTES embedded in a matrix that includes CHONDROITIN SULFATE and various types of FIBRILLAR COLLAGEN. There are three major types: HYALINE CARTILAGE; FIBROCARTILAGE; and ELASTIC CARTILAGE.Gastric Mucins: Mucins that are found on the surface of the gastric epithelium. They play a role in protecting the epithelial layer from mechanical and chemical damage.Hyaluronic Acid: A natural high-viscosity mucopolysaccharide with alternating beta (1-3) glucuronide and beta (1-4) glucosaminidic bonds. It is found in the UMBILICAL CORD, in VITREOUS BODY and in SYNOVIAL FLUID. A high urinary level is found in PROGERIA.Chondrocytes: Polymorphic cells that form cartilage.Goblet Cells: A glandular epithelial cell or a unicellular gland. Goblet cells secrete MUCUS. They are scattered in the epithelial linings of many organs, especially the SMALL INTESTINE and the RESPIRATORY TRACT.Mast Cells: Granulated cells that are found in almost all tissues, most abundantly in the skin and the gastrointestinal tract. Like the BASOPHILS, mast cells contain large amounts of HISTAMINE and HEPARIN. Unlike basophils, mast cells normally remain in the tissues and do not circulate in the blood. Mast cells, derived from the bone marrow stem cells, are regulated by the STEM CELL FACTOR.Microscopy, Electron: Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.Sublingual Gland: A salivary gland on each side of the mouth below the TONGUE.Chondroitin Sulfates: Derivatives of chondroitin which have a sulfate moiety esterified to the galactosamine moiety of chondroitin. Chondroitin sulfate A, or chondroitin 4-sulfate, and chondroitin sulfate C, or chondroitin 6-sulfate, have the sulfate esterified in the 4- and 6-positions, respectively. Chondroitin sulfate B (beta heparin; DERMATAN SULFATE) is a misnomer and this compound is not a true chondroitin sulfate.Exocrine Glands: Glands of external secretion that release its secretions to the body's cavities, organs, or surface, through a duct.Glycoconjugates: Carbohydrates covalently linked to a nonsugar moiety (lipids or proteins). The major glycoconjugates are glycoproteins, glycopeptides, peptidoglycans, glycolipids, and lipopolysaccharides. (From Biochemical Nomenclature and Related Documents, 2d ed; From Principles of Biochemistry, 2d ed)Collagen Type II: A fibrillar collagen found predominantly in CARTILAGE and vitreous humor. It consists of three identical alpha1(II) chains.Proteoglycans: Glycoproteins which have a very high polysaccharide content.Dog Diseases: Diseases of the domestic dog (Canis familiaris). This term does not include diseases of wild dogs, WOLVES; FOXES; and other Canidae for which the heading CARNIVORA is used.Histological Techniques: Methods of preparing tissue for examination and study of the origin, structure, function, or pathology.Hyaluronoglucosaminidase: An enzyme that catalyzes the random hydrolysis of 1,4-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate. (From Enzyme Nomenclature, 1992) There has been use as ANTINEOPLASTIC AGENTS to limit NEOPLASM METASTASIS.Aggrecans: Large HYALURONAN-containing proteoglycans found in articular cartilage (CARTILAGE, ARTICULAR). They form into aggregates that provide tissues with the capacity to resist high compressive and tensile forces.Immunohistochemistry: Histochemical localization of immunoreactive substances using labeled antibodies as reagents.Connective Tissue: Tissue that supports and binds other tissues. It consists of CONNECTIVE TISSUE CELLS embedded in a large amount of EXTRACELLULAR MATRIX.Cartilage, Articular: A protective layer of firm, flexible cartilage over the articulating ends of bones. It provides a smooth surface for joint movement, protecting the ends of long bones from wear at points of contact.Trypan Blue: A diazo-naphthalene sulfonate that is widely used as a stain.

Changes in joint cartilage aggrecan after knee injury and in osteoarthritis. (1/131)

OBJECTIVE: To determine the concentrations of aggrecan fragments in synovial fluid from patients with knee joint injury, osteoarthritis (OA), or acute pyrophosphate arthritis (PPA; pseudogout), and to test their relative reactivity with the 846 epitope, a putative marker of cartilage aggrecan synthesis. METHODS: Samples of knee joint fluid from 385 patients and 9 healthy-knee volunteers were obtained in a cross-sectional study. Study groups were acute PPA/ pseudogout (n = 60), anterior cruciate ligament (ACL) rupture (n = 159), meniscus lesion (n = 129), and primary knee OA (n = 37). The 846 epitope on aggrecan was assayed by competitive solution-phase radioimmunoassay. Aggrecan fragments were assayed by enzyme-linked immunosorbent assay using a monoclonal antibody (1-F21). Cartilage oligomeric matrix protein (COMP), C-propeptide of type II collagen (CPII), bone sialoprotein, matrix metalloproteinases 1 and 3, and tissue inhibitor of metalloproteinases 1 were previously quantified by immunoassays. RESULTS: Reactivity of the 846 epitope was increased in all study groups compared with the reference group, and was highest in patients with primary OA. The median levels (in microg fetal aggrecan equivalents/ml) of the epitope were 0.28 (range 0.24-0.47) in the reference group, 0.48 (range 0.26-1.32) in PPA/pseudogout, 0.61 (range 0.12-2.87) in ACL rupture, 0.53 (range 0.22-3.02) in meniscus lesion, and 0.68 (range 0.31-4.31) in primary OA. The 846 epitope reactivity per microg aggrecan fragments in the joint fluid was higher in late-stage OA than in early-stage OA. Epitope 846 reactivity correlated positively with several markers of matrix turnover, particularly with COMP (r(s) = 0.421) and CPII (r(s) = 0.307). CONCLUSION: The observed differences in 846 epitope reactivity in synovial fluid, and its concentration in relation to aggrecan and other markers of matrix turnover, were consistent with marked ongoing changes in aggrecan turnover after joint injury and in the development of OA. OA is thus a disease characterized by dynamic changes in tissue macromolecule turnover, which is reflected by measurable changes in aggrecan epitopes in the synovial fluid.  (+info)

Differences in the acid-labile component of Candida albicans mannan from hydrophobic and hydrophilic yeast cells. (2/131)

Cell surface hydrophobicity of the opportunistic fungal pathogen Candida albicans has been linked to the level of cell wall protein glycosylation. Previous work demonstrated that outer chain mannosylation, rather than overall glycosylation, correlated with cell surface hydrophobicity. These studies further suggested that the phosphodiester-linked, acid-labile beta-1,2-mannan was the correlating element. The present work tests this hypothesis and extends the previous results. The composition of bulk mannan from hydrophobic and hydrophilic yeast cells, and the acid-labile mannan from both cell types are compared. Compositional analysis shows that the protein, hexose, and phosphorus content of bulk mannan is similar between the two phenotypes. Electrophoretic separation of acid-released and fluorophore-labeled mannan shows that the acid-labile oligomannosides from hydrophobic cells are longer and potentially in greater abundance than those from hydrophilic cells. These results suggest that regulation of a single step in cell wall protein outer chain mannosylation affects the cell surface ultrastructure and phenotype of C.albicans.  (+info)

Induction of dog sperm capacitation by glycosaminoglycans and glycosaminoglycan amounts of oviductal and uterine fluids in bitches. (3/131)

Ejaculated sperm collected from 12 beagle dogs were incubated in canine capacitation medium (CCM), supplemented with 5 microg/ml chondroitin sulfate A (CS), 5 microg/ml hyaluronic acid (HA), or 5 microg/ml heparin (HP) for 7 hr at 38 degrees C in a 5% CO2 in air atmosphere to investigate the effects of glycosaminoglycans (GAGs) on dog sperm capacitation. The percentages of motile sperm, hyperactivated sperm (%HY), and acrosome-reacted sperm (%AR) in all media were examined after 4 hr and 7 hr of incubation. The oviducts and uteri of 9 anestrous and 18 estrous beagle bitches were removed under halothane inhalation anesthesia to measure the total GAG amounts in oviductal and uterine fluids. The lumens of the ampulla of the oviducts, isthmus of the oviducts, and the uterine horns were each flushed with 1 ml HEPES-EDTA fluid. Total GAG amounts in the flush fluids obtained were measured with a spectrophotometer. Sperm motility (51-59%), %HY (79-86%), and %AR (31-36%) in CCM supplemented with CS, HA, or HP were significantly higher after 7 hr of incubation than when incubated in CCM without GAGs (P<0.01 or 0.05). The mean total GAG amounts in the fluids from the ampulla and isthmus of the oviducts and the uterine horns in the estrous bitches were higher than in the anestrous bitches. These results indicate that GAGs in the oviductal and uterine fluids in estrous bitches are associated with in vivo sperm capacitation.  (+info)

Mapping metastases in sentinel lymph nodes of breast cancer. (4/131)

Localization of metastases within the sentinel lymph nodes (SLNs) of breast cancer has not been studied. Forty SLNs from 36 patients with operable primary breast cancers were identified by means of lymphatic mapping with patent blue dye. The junction between the patent blue-stained lymphatic vessel draining the tumor and the SLN was labeled with alcian blue. Metastases within the serially sectioned SLNs were assigned to the alcian blue-labeled side, to the opposite side of the virtually halved nodes, or both. Eight SLNs were negative for metastasis. Eleven SLNs had metastases only in the blue half. Only 4 cases had larger metastases in the nonblue half. Metastases are more likely to be located in the vicinity of the inflow junction of the identifiable lymphatic draining the tumor and the SLN. This should be considered when SLNs are examined, especially when they are halved for different studies.  (+info)

The pathogenesis of duodenal gastric metaplasia: the role of local goblet cell transformation. (5/131)

BACKGROUND AND AIMS: Gastric metaplasia is frequently seen in biopsies of the duodenal cap, particularly when inflamed or ulcerated. In its initial manifestation small patches of gastric foveolar cells appear near the tip of a villus. These cells contain periodic acid-Schiff (PAS) positive neutral mucins in contrast with the alcian blue (AB) positive acidic mucins within duodenal goblet cells. Previous investigations have suggested that these PAS positive cells originate either in Brunner's gland ducts or at the base of duodenal crypts and migrate in distinct streams to the upper villus. To investigate the origin of gastric metaplasia in superficial patches, we used the PAS/AB stain to distinguish between neutral and acidic mucins and in addition specific antibodies to immunolocalise foveolar cell mucin MUC5AC, the foveolar cell secretory product, gastric trefoil factor (TFF1), the mature goblet cell mucin MUC2, and MUC2 core antigen. RESULTS: Cells in focal patches of gastric metaplasia contained secretory granules of both gastric and goblet cell phenotypes. MUC5AC and TFF1 were present as expected in gastric foveolar cells but in addition, MUC2 core antigen, normally present only in the Golgi of intestinal goblet cells, was expressed in secretory granules. Goblet cells in the vicinity of metaplastic patches also expressed both gastric and intestinal antigens. MUC5AC/MUC2 containing goblet cells were most common near the villus tip but were also seen at the base of crypts. Where crypts and Brunner's gland ducts merged they were always seen on the crypt side of the junction. Goblet cells were the only cells to express gastric antigens in these areas. In advanced metaplastic lesions, dual phenotype goblet cells were less evident and fewer cells expressed intestinal mucin antigens. CONCLUSIONS: We suggest that goblet cells that express both intestinal and gastric antigens may represent local precursors of gastric metaplasia undergoing a transition to foveolar-like cells of mixed phenotype at the site of early metaplastic patches. As metaplasia becomes more widespread, a more pure gastric phenotype emerges. This progression is likely to be controlled by local inflammatory signals.  (+info)

Development of conjunctival goblet cells and their neuroreceptor subtype expression. (6/131)

PURPOSE: To investigate expression of muscarinic, cholinergic, and adrenergic receptors on developing conjunctival goblet cells. METHODS: Eyes were removed from rats 9 to 60 days old, fixed, and used for microscopy. For glycoconjugate expression, sections were stained with Alcian blue/periodic acid-Schiffs reagent (AB/PAS) and with the lectins Ulex europeus agglutinin I (UEA-I) and Helix pomatia agglutinin (HPA). Goblet cell bodies were identified using anti-cytokeratin 7 (CK7). Nerve fibers were localized using anti-protein gene product 9.5. Location of muscarinic and adrenergic receptors was investigated using anti-muscarinic and beta-adrenergic receptors. RESULTS: At days 9 and 13, single apical cells in conjunctival epithelium stained with AB/PAS, UEA-I, and CK7. At days 17 and 60, increasing numbers of goblet cells were identified by AB/PAS, UEA-I, HPA, and CK7. Nerve fibers were localized around stratified squamous cells and at the epithelial base at days 9 and 13, and around goblet cells and at the epithelial base at days 17 and 60. At days 9 and 13, M2- and M3-muscarinic and beta2-adrenergic receptors were found in stratified squamous cells, but M1-muscarinic and beta1-adrenergic receptors were not detected. At days 17 and 60, M2- and M3-muscarinic receptors were found in goblet cells, whereas M1-muscarinic receptors were in stratified squamous cells. Beta1- and beta2-adrenergic receptors were found on both cell types. Beta3-adrenergic receptors were not detected. CONCLUSIONS: In conjunctiva, nerves, M2- and M3-muscarinic, and beta1- and beta2-adrenergic receptors are present on developing goblet cells and could regulate secretion as eyelids open.  (+info)

Dynamic regulation of mucus gel thickness in rat duodenum. (7/131)

We examined the dynamic regulation of mucus gel thickness (MGT) in vivo in rat duodenum in response to luminal acid, cyclooxygenase (COX) inhibition, and exogenous PGE(2). An in vivo microscopic technique was used to measure MGT with fluorescent microspheres in urethan-anesthetized rats. Duodenal mucosa was topically superfused with pH 7.0 or pH 2.2 solutions with or without PGE(2) and indomethacin treatments. Glycoprotein concentration of duodenal loop perfusates was measured with periodic acid/Schiff (PAS) or Alcian blue (AB) staining. MGT and perfusate glycoprotein concentration were stable during a 35-min perfusion with pH 7.0 solution. Acid exposure increased MGT and PAS- and AB-positive perfusate glycoprotein concentrations. Indomethacin pretreatment increased both PAS- and AB-positive perfusate glycoprotein at baseline; subsequent acid superfusion decreased perfusate glycoproteins and gel thickness. PGE(2) (1 mg/kg iv) simultaneously increased MGT and PAS-positive perfusate glycoprotein concentrations followed by a transient increase in AB-positive glycoprotein concentration, suggesting contributions from goblet cells and Brunner's glands. Parallel changes in MGT and perfusate glycoprotein concentration in response to luminal acid and PGE(2) suggest that rapid MGT variations reflect alterations in the balance between mucus secretion and exudation, which in turn are regulated by a COX-related pathway. Luminal acid and PGE(2) augment mucus secretion from goblet cells and Brunner's glands.  (+info)

Genetic control of yeast mannan structure: mapping genes mnn2 and mnn4 in Saccharomyces cerevisiae. (8/131)

Two mutations concerned with mannan biosynthesis in the yeast Saccharomyces cerevisiae have been mapping. The mnn2 mutation, which affects the addition to the polysaccharide backbone of the first side-chain D-mannose unit in alpha1-leads to2 linkage, was located on chromosome II linked to the centromere and the gall locus. The mn4 locus, which regulates the synthesis of mannosylphosphate groups on the mannan side chains, was placed on chromosome XI near trp3 and ural and a locus previously reported to regulate the ability of a S. diastaticus strain to bind alcian blue (Friis and Ottolenghi, 1970). The mnn4 mutant also fails to bind alcain blue, but the gene responsible for alcian blud binding in this strain segregates independently from the dye-binding locus of S. diastaticus, and therefore must be a different gene. A diploid heterozygous for mnn4 fails to bind dye, indicating dominance of this mutant genotype. The alcian blue dye binding locus dbll, reported to Friis and Ottolenghi (1970), is also dominant. Thus, there are at least two independent genes that control the formation of the mannosylphosphate units in the mannan side chains, and both have the property of dominance in the mutant form.  (+info)

*Periodic acid-Schiff stain

Alcian blue/periodic acid-Schiff (AB/PAS or AB-PAS) uses alcian blue before the PAS step. PAS staining is mainly used for ... dyes Methyl blue Methyl violet Methylene blue New methylene blue Potassium ferricyanide Potassium ferrocyanide Prussian blue ... Carboxyfluorescein DyLight Fluor, a product line of fluorescent dyes Egyptian blue Eosin Erythrosine Fluorescein Fluorescein ...

*Alcian blue stain

Alcian blue or alcian blue (/ˈælʃən/) is any member of a family of polyvalent basic dyes, of which the Alcian blue 8G (also ... Solid Alcian blue is a combustible powder and should never be handled close to heat or a naked flame. Heating Alcian blue ... In addition to its use as a dye or stain Alcian blue also finds other material science uses. Alcian blue has been used as an ... Scott also states that Alcian green was merely a mixture of Alcian blue and Alcian yellow and not a single compound, which is ...

*Alcian Blue (band)

Alcian Blue was a shoegaze and new wave-influenced band operating out of the Washington, DC suburb of Takoma Park from 1998 to ... "Alcian Blue - Alcian Blue - Songs, Reviews, Credits - AllMusic". AllMusic. Retrieved 2016-08-09. http://www.safraninsound.com/ ... Slow Colorless Stare CD (2001, Safranin Sound) Alcian Blue CD (2006, Elephant Stone) Angelica Take Me Down EP (2003, Safranin ...

*Stratified squamous epithelium

Alcian blue stain. Tortora, Gerard J.; Derrickson, Bryan. Introduction to the Human Body. The Essentials of Anatomy and ...

*Multiple endocrine neoplasia type 2b

Early lesions, rich in acid mucopolysaccharides, stain positively with alcian blue. When medullary thyroid cancer is present, ... Luxol fast blue staining identifies myelin sheathing of some fibers, and lesional cells react immunohistochemically for S-100 ...

*Ectomesenchymal chondromyxoid tumor

Alcian blue will give a blue staining to the stromal matrix material. Mucicarmine will also highlight the stromal matrix (pink ...

*Movat's stain

... alcian blue, Verhoeff hematoxylin, crocein scarlet combined with acidic fuchsine and saffron. At pH 2.5, alcian blue is fixed ... Movat's stain showing thickening of the spongiosa layer (blue) in myxomatous degeneration of the aortic valve. Movat's stain ...

*Barrett's esophagus

Use of the histochemical stain Alcian blue pH 2.5 is also frequently used to distinguish true intestinal-type mucins from their ...

*Alcian yellow

... shades of blue Alcian blue stain Leung, Jennifer K.; Gibbon, Kevin J.; Vartanian, Robert K. (1996-06-01). "Rapid Staining ... Alcian yellow is used in histology in conjunction with toluidine blue to help visualize H. pylori. The resulting structures are ... Alcian yellow is an azo dye with the formula (CH3[(CH3N)2CS]2C6H2SNCC6H4)2N2. Its structure consists of a largely conjugated ... stained as follows: - Mucin: yellow - H. pylori: blue - Other cellular components/backgroun: ...

*Mucoepidermoid carcinoma

Alcian blue-PAS stain Elsevier Article Locator Modern Pathology - Primary Mucoepidermoid Carcinoma and Sclerosing ...

*Van Gieson's stain

Other dyes used in connection with van Gieson staining include: Alcian blue Amido black 10B Verhoeff's stain Jocelyn H. Bruce- ...

*List of MeSH codes (D03)

... alcian blue MeSH D03.438.473.133 --- captan MeSH D03.438.473.155 --- carbolines MeSH D03.438.473.193 --- chlorisondamine MeSH ... methylene blue MeSH D03.494.741.533 --- moricizine MeSH D03.494.741.550 --- nonachlazine MeSH D03.494.741.575 --- perazine MeSH ...

*Glycocalyx

When vessels are stained with cationic dyes such as Alcian blue stain, transmission electron microscopy shows a small, ...

*List of shoegazing musicians

Adorable Air Formation Airiel Alcian Blue Alison's Halo Apollo Heights Asobi Seksu Astreal The Away Days The Boo Radleys The ...

*Elephant Stone Records

Alcian Blue "Alcian Blue" ES1014 - Ashley Naylor "Ashley Naylor's Four Track Mind" ES1013 - The Situation "The Situation" ...
0, and good lipid stains generally have a LogP>+7). Methanol is an acceptable substitute for ethanol as a potential vehicle for Alcian blue, but isopropanol is not, because, within a few hours all of suspended Alcian blue precipitates if isopropanol is tried as a vehicle. The sample compound with Merck index number 218 has a melting point of 148 °C. It is a tetravalent basic (cationic) dye with a copper (Cu2+, coordination 4 of 6, orbital configuration d9 with Jahn-Teller distortion) phthalocyanine nucleus (CuPc) with three or four pendent isothiouronium side chains imparting its bulkiness and positive charges. In order to qualify as an alcian blue family member there has to be at least 2 side chains and the mixtures often have 3 chains in average to qualify as 8G. Four tetramethylisothiouronium groups per molecule are shown in the picture. ICI had claimed an average of about three side chains per molecule, but analyses by Prof Scotts lab suggested between three and four. Most of them are at ...
Hi Tom: Alcian Blue will stain the glycosaminoglycans in the extracellular matrix, it wont show much cellular morphology. Still, the traditional method is 1% Alcian Blue in 3% acetic acid for a pH of 2.5. At 2.5 both sulfated and carboxylated GAGs are stained. A red nuclear stain, Scarba red or nuclear fast red, gives a nice contrasty counterstain. You might try 0.05% Toluidine blue in dist water with a pH of about 4.0 for 10-15 minutes, dist water rinse and dehydration in 3 changes of actone followed by clearing in fresh xylene. Nice metachromasia of=20 extracellular matrix and you will see some cellular morphology as well. Geoff Tom Schaer wrote: >Greetings List: > >I would like to stain some intervertebral disc x-sections (rat, decalcified) with alcian blue. Interest in cellular morphology of nucleus pulposus, transition and annulus cells. > >What pH of the stain should I use. > >Many thanks, > >tom >-------------------------------------------------- >Thomas P. Sch r, VMD >Asst Prof School ...
Thanks to all who responded to the Alcian blue question. It is definitely a developmental problem with these bones/cartilage. The cells are not producing the required muccopolysaccharides necessary for proper staining. anita ...
ABSTRACT: Microphytobenthos (MPB) are an important component of shallow-water habitats where there is sufficient light to support benthic photosynthesis. Production of extracellular polymeric substances (EPS) is characteristic of the MPB, and acid polysaccharides (APS) are a major component of these EPS. A simple technique was developed to extract and measure APS concentrations in sediments. Water-extractable APS (wAPS) were extracted with ultra high purity water from lyophilized sediments. After centrifuging to remove the sediment, wAPS in the supernatant was precipitated with alcian blue, a cationic dye. The APS-alcian blue precipitate was removed by filtration. The remaining alcian blue in solution was determined spectrophotometrically and was inversely proportional to the concentration of wAPS in the sediment. Chlorophyll (chl) a, carbohydrate fractions, and wAPS were measured in sediments from 5 contrasting sites in a microtidal salt marsh in the subtropics. wAPS concentrations, expressed ...
Posterior salivary glands. As the gland described above, posterior salivary glands are simple branched tubular glands. They are covered by a connective capsule with muscular fibers surrounding the glandular tubules like thin connections. Each tubule presents two zones formed by different cells. The first or proximal zone (A) consists of globular mucous cells with flattened basal nuclei (Figure 5b). The supranuclear cytoplasm contains high level of PAS (+) and alcian blue (+) pH = 3.5, vesicles denoting the presence of weakly sulphated glycosaminoglycans content. Cylindrical cells with a basal spherical nucleus surrounded by a basophilic cytoplasm are also seen. The second or distal zone (B) present cylindrical cells characterized by its weakly eosinophilic cytoplasm. The nucleus with basal or apical location is observed (Figure 5b). The PAS (+) and Alcian Blue (-) pH = 3.5 reaction in the cytoplasm, suggests the presence of neutral carbohydrates.. Animals kept in the aquarium without food for ...
PAVON, L. F.... Ultrastructural characterization of CD133(+) stem cells bound to superparamagnetic nanoparticles: possible biotechnological applications. JOURNAL OF MICROSCOPY 231 n.3 p. 374-383 SEP 2008. Journal article.
Scores of people around the country are protesting against Drax Plc on the day of its AGM today, with three simultaneous demonstrations in London, Liverpool and York. Campaigners are highlighting Draxs involvement in dirty energy, its contributions to… Read More. ...
INTRODUCTION: The clinical efficacy of infliximab (IFX) for induction of remission in both adults and children with active Crohns disease (CD) has been well documented. Recently, so-called "deep remission" defined as mucosal healing has become the ultimate endpoint of the most recent therapeutic advances for CD. However, endoscopic evidence of mucosal healing is not necessarily associated with histological evidence of suppression of inflammation. AIM: Since data on that issue are limited, especially in the paediatric population, the aim of this study was to assess the impact of induction therapy with IFX on deep microscopic remission in paediatric patients with CD ...
D: The skeleton of a wild-type mouse (right) and Wwtr1tm1Whun/Wwtr1tm1Whun mouse (left) embryos stained with Alizarin red and Alcian blue to stain bone (red) and cartilage (blue), respectively. Note the slightly shorter skeleton of the homozygous mouse embryo. G & H: Hematoxylin and eosin staining of longitudinal kidney sections from 8 week old wild-type (G) and homozygous mutant mouse (H), showing numerous cysts in the corticomedullary region of the homozygous mouse kidney. Magnification: x20 ...
FIG. 3. Whole mount in situ hybridization pattern of Xbap [cc: now called nkx3-1] (A, B, C, D, E, and F) and gsc (G, H, I, J, and K) and Alcian blue staining to visualize cartilage (L). All embryos were photographed at 701 magnification except for (D) at 601. (A) Lateral view of a stage 36 embryo showing both regions of facial staining and the region of gut expression (indicated by g in the figure). (B) Anterior view of the embryo pictured in (A). The paired anterior regions of expression lie on either side of the future mouth opening (M). (C) Dorsal view of a stage 37 embryo showing the forked appearance of the Xbap staining. (D) Lateral view of the left side of a stage 42 embryo showing staining of the anterior gut tube and facial staining. (E) Dorsal view of a stage 40 embryo. Xbap staining now marks crescent-shaped regions on either side of the future mouth opening (M). (F) Dorsal view of the embryo shown in (D). The region of staining corresponding to the palatoquadrate cartilage (PQ) is ...
Hyaluronate electophoresis - posted in Biochemistry: Hi everyone! I would like to ask if anyone has any experience in electrophoresis and detection of hyaluronate. I did PAGE of a digest mixture (consisting of HA chains of various lengths) according to numerous protocols (15% and 10% gel in TBE, linear gradient 20%-3% and 18%-1,5%, with samples in NaOAc, NH4HCO3, NaOAc+HCl, dH20 or TBE, with the voltage of 300V and 100V and detection by Alcian Blue in water, water+2% acetic acid or wat...
The colour database is collected and created by the experts of Chemnet. There are more than 1,000 colours in this database about the colours physicochemical property, applications, synthetic methods and so on. It will be updated in time.
Hito Alcian Blue pH-2.5 OptimStain™ Kit is formulated for the staining of acid mucins, sulfated / carboxylated acid mucopolysaccharides and sulfated / carboxylated sialomucins (glycoproteins).
Orcein-alcian blue staining, a new method for the simultaneous demonstration of sulphated and sialomucins in gastrointestinal epithelium was compared with the standard high iron diamine-alcian blue technique. Sections were oxidised with potassium permanganate and decolourised in oxalic acid. They were stained with orcein for four hours, differentiated for a few seconds in acid alcohol, and then counterstained with alcian blue for half to one minute. There was a good correlation of results between the two methods. Orcein-alcian blue is a safer, cheaper, and quicker method than high iron diamine-alcian blue which can be safely introduced into routine laboratories for the study of acid mucins in the gastrointestinal diseases.. ...
Looking for online definition of Blue Cell Tumor in the Medical Dictionary? Blue Cell Tumor explanation free. What is Blue Cell Tumor? Meaning of Blue Cell Tumor medical term. What does Blue Cell Tumor mean?
Images: Cuboidal lining , Glycogen , Aggressive serous cyst , Cysts in serous cystadenomas are composed of a single layer of clear cuboidal cells. These cells usually form flat sheets that line the cysts and only rarely form papillae that project into the cystic spaces. The neoplastic cells have clear cytoplasm because they contain abundant intracytoplasmic glycogen. They do not produce mucin. The nuclei are small, round, and have uniform chromatin and inconspicuous nucleoli. Atypia and mitoses are essentially absent. The central stellate scar and the stroma separating the cysts are composed mostly of relatively acellular collagenous connective tissues, but the stroma may contain entrapped islets of Langerhans and acini. Special stains will highlight the abundant glycogen and absence of mucin. A periodic acid Schiff (PAS) stain will be strongly positive and sensitive to diastase digestion. Stains for mucin, including a mucicarmine and alcian blue stains, will be negative, as will stains for ...
Periodic acid-Schiff (PAS) is a staining method used to detect polysaccharides such as glycogen, and mucosubstances such as glycoproteins, glycolipids and mucins in tissues. The reaction of periodic acid oxidizes the vicinal diols in these sugars, usually breaking up the bond between two adjacent carbons not involved in the glycosidic linkage or ring closure in the ring of the monosaccharide units that are parts of the long polysaccharides, and creating a pair of aldehydes at the two free tips of each broken monosaccharide ring. The oxidation condition has to be sufficiently regulated so as to not oxidize the aldehydes further. These aldehydes then react with the Schiff reagent to give a purple-magenta color. A suitable basic stain is often used as a counterstain. PAS diastase stain (PAS-D) is PAS stain used in combination with diastase, an enzyme that breaks down glycogen. Alcian blue/periodic acid-Schiff (AB/PAS or AB-PAS) uses alcian blue before the PAS step. PAS staining is mainly used for ...
Histochemical Special Stains •Acid-fast Bacillus, Kinyouns •Alcian blue / PAS •Alcian Blue •Amyloid, Congo Red •CAE (Chloroacetate esterase)
Demonstrates neutral and acidic mucosubstances on tissue at pH 2.5 imparts a blue color to the acidic mucins and other carboxylated or weakly sulphated acid mucosubstances. Periodic Acid Schiff (PAS) reaction is then used to stain basement membranes, glycogen and neutral mucosubstances pink to red. Mixtures of neutral and acidic mucosubstances will appear purple due to positive reactions with both Alcian Blue and PAS.. Learn More ...
Demonstrates neutral and acidic mucosubstances on tissue at pH 2.5 imparts a blue color to the acidic mucins and other carboxylated or weakly sulphated acid mucosubstances. Periodic Acid Schiff (PAS) reaction is then used to stain basement membranes, glycogen and neutral mucosubstances pink to red. Mixtures of neutral and acidic mucosubstances will appear purple due to positive reactions with both Alcian Blue and PAS.. Learn More ...
Posterior gut (Alcian Blue-PAS) from fish fed (A) Fish oil-based diet (FO), (B) FO- and MOS-based diet (FOMOS), (C) soy bean meal-based diet (SBO), and (D) SBO-
Checkpoint inhibitors, including nivolumab and pembrolizumab, are novel therapies that have demonstrated significant efficacy in the management of metastatic malignancy.10 The immunological adverse event profile related to these therapies is broad, with rates of enterocolitis as high as 30%.7,11 The median time of onset of symptoms is 6-7 weeks from initiation of therapy but can vary, with some reports as late as 6 months, as in our case.11 The colitis is manifested by diarrhea, defined as greater than 4 stools per day, with blood or mucous, abdominal discomfort or cramping, and radiographic or endoscopic evidence of inflammation.12 The pathophysiology is characterized by lymphocytic infiltration in the lamina propria and epithelium of the involved mucosa. This inflammation can be transmural, and spontaneous bowel perforations have been reported.13 Interestingly, the remainder of the GI tract is rarely involved. Management includes exclusion of other infectious etiologies followed by initiation ...
Histology: the tumour has an expansive growing pattern mainly surrounded by fibrous tissue and it is composed by solid and glandular/pseudoglandular components. The cells are small, myoepithelial and epithelial cells, polarized around two types of spaces - glandular or pseudolumina. The true glandular spaces are surrounded by luminal cells and contain neutral mucin (PAS positive); the pseudolumina are stromal spaces filled by cylinders of hyaline material, surrounded by basal/myoepithelial cells; these spaces stain for Alcian Blue and collagen IV. The cells in the solid areas and around the cylinders stain for p63, smooth-muscle actin as well as for high-molecular-weight keratins. Cells around the lumina stain for low-molecular-weight keratin (for example keratin 7), as well as for CD117. On the cystic component, squamous metaplasia can be observed. Focally mainly in the solid areas the cells are bigger with evident nucleoli and the mitotic index is higher. Necrotic foci can be observed ...
The Alcian Blue (pH 2.5) Stain Kit is intended for use in the histological visualization of sulfated and carboxylated acid mucopolysaccharides and sulfated and carboxylated sialomucins (glycoproteins ...
산약 (Dioscoreae Rhizoma)의 수용성 점액물질(mucilage)은 Polysaccharide와 Protein으로 구성되어 있는 proteoglycan으로 알려져 왔다. 본 연구에서는 종래의 mucilage 정제법인 에탄올 침전법과는 다른 새로운 방법으로 mucilage 정제를 실시하고자 하였으며, 산약의 mucilage에 대한 정량법을 확립하고, 산약의 가공에 따른 mucilage 함량변화를 알아보았다. 시료산약은 재배품종으로서 시판되고 있는 두 가지 형태의 마 (곤봉형 마, 杖형 마)를 사용하였고, 각각의 생품과 증건품을 가지고 실험하였다. 산약의 물 추출액을 투석한 추, Bio-gel P-100과 Sephadex G-150을 사용하여 겔 여과한 결과, polysaccharide 부분과 Protein 부분이 서로 분리되어 나타나는 것으로 보아, 산약의 mucilage 에는 Protein이 함유되어 있지 않음을 확인할 수 있었다. 한편, mucilage 정량 방법으로는 산약의 mucilage성분이 alcian blue 와 ...
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The investigators expect to enroll 18 participants with IgM-associated AL amyloidosis onto this Phase II clinical trial. Idelalisib will be self-administered
This FlowCellectMitoDamage Kit for Flow Cytometry includes MitoSense Red, a fluorescent cationic dye that accumulates in the mitochondria & is responsive to mitochondrial potential changes. Find MSDS or SDS, a COA, data sheets and more information.
Just to clarify for anyone that is unfamiliar, phagocytosis is the process in which one cell engulfs another. In this case, the big, blue cell is about to swallow the little, purple cell ... And the purple cell is thinking: "Oh, no ...
Looking for online definition of basic fuchsin-methylene blue stain in the Medical Dictionary? basic fuchsin-methylene blue stain explanation free. What is basic fuchsin-methylene blue stain? Meaning of basic fuchsin-methylene blue stain medical term. What does basic fuchsin-methylene blue stain mean?
Multiple specimens taken at oesophageal suction biopsy were obtained from 56 patients, of whom 44 had symptoms of gastro-oesophageal reflux and 24 had endoscopic evidence of erosive oesophagitis. Biopsies were examined independently by two histopathologists for the following criteria for reflux: epithelial hyperplasia, vascular dilatation and congestion, neutrophil infiltration, and eosinophil infiltration. The incidence of these criteria in patients with and without endoscopic evidence of oesophagitis or symptoms of reflux was investigated. It was concluded that vascular dilatation and epithelial hyperplasia, defined as basal zone thickness greater than or equal to 15% and papillary elongation greater than or equal to 66%, can be detected most reliably, but their diagnostic accuracy is limited unless multiple biopsies are examined.. ...
The cationic dye, chrysoidine, has been used for the first time as a probe for the indirect photometric detection of cations. The dye has been used as a probe at concentrations of 5 mM, which is roughly an order of magnitude higher than for other cationic dyes used previously for the same purpose, in order to minimise electromigrational dispersion. Baseline instability was minimised by a combination of coating the capillary with poly(ethyleneimine), addition of a neutral polymer to the electrolyte, and the application of a small amount (20 mbar) of hydrodynamic pressure during the separation. Separation of a mixture containing alkali metals, alkaline earths, transition metals and lanthanides was achieved by the addition of 2-hydroxyisobutyric and lactic acid as complexing agents. Excellent peak shapes were observed over a wide range of analyte mobilities due to the moderate mobility of the probe. The high absorptivity (26 733 l mol−1 cm−1) provided by chrysoidine in comparison with typically ...
Pig duodenum stained to differentiate carboxylated mucus in Brunners glands (yellow) from sulfonated mucus in gobletcells (blue-green). Alcian blue pH 1.0 + periodic acid + sodium metabisulfite + Alcian yellow = Brazilliant ...
Patients should have had a liver biopsy showing at least Stage 3 disease prior to being considered for this study. A baseline liver biopsy is necessary for inclusion in the study. Baseline liver biopsies can be performed within six months of entering the study.. In patients with cirrhosis and endoscopic evidence of portal hypertension, a biopsy within the last 2 years is acceptable as the baseline biopsy. For patients with established cirrhosis on liver biopsy and no portal hypertension, a biopsy within 12 months can be used as the baseline biopsy if it is available for evaluation by the Pathology core. All these patients will still require liver biopsy at 2 years and 4 years. The decision to biopsy at 2 and 4 years is also a clinical decision and in the presence of clinical progression or coagulopathy, or where there may be a risk from liver biopsy, the Investigator should call the PI, Dr. Afdhal for a waiver of biopsy. Patients with Ishak Stage 3 and 4 require a biopsy within 6 months of ...
In the gastric mucosa of a patient with Helicobacter pylori infection and a precancerous lesion called "incomplete intestinal metaplasia" (Genta stain), the gastric cells lining a gland have been replaced by absorptive intestinal cells with visible brush border and goblet cells, which are normally present in the intestine, but not in the stomach. Goblet cells contain acidic mucins stained blue by Alcian blue. This type of precancerous transformation is caused by long-term infection with H. pylori, visible in the lumen of the gland as curved bacterial rods stained black by silver stain. A few bacteria invade the goblet cells and can be found within blood capillaries, where they bind to red blood cells (see Aspholm et al. 2006).. ...
The fixation of dissociated chick embryonic cells in the presence of ruthenium red or alcian blue revealed a remarkable reduction in the binding of these cations to cell surfaces following tryptic digestion at 37 °C, but not after trypsin used at 4 °C or EDTA.. The reconstitution of anionic sites present over the cell plasma membrane was studied in retina, liver and heart cells from embryos of different ages, cultivated according to a method which promotes cell reaggregation. All these cells regenerated ruthenium red-stainability within the first hour of culture. Culturing at 15 °C, substitution of saline for the culture medium or addition of puromycin or of neuraminidase to the culture media did not affect this restitutive process. Neuraminidase and hyaluronidase digestion were not able to alter the ruthenium red-binding, once it was present.. From these data and from the reports available in the literature it has been concluded that the anionic binding-sites cannot be identified with the ...
* found in: Methyl Orange, Trypan Blue Solution 0.4%, Xylene Cyanol FF, Neutral Red, Safranin O, Alcian Blue 8 GX, Nitroblue Tetrazolium Chloride, Congo..
In cultured murine costal chondrocytes, mRNA was constitutively expressed for metabotropic GABA(B) receptor subunit-1 (GABA(B)R1), but not for GABA(B)R2. Immunohistochemical analysis revealed the predominant expression of GABA(B)R1 by prehypertrophic to hypertrophic chondrocytes in tibial sections of newborn mice. The GABA(B)R agonist baclofen failed to significantly affect chondrocytic differentiation determined by Alcian blue staining and alkaline phosphatase activity in cultured chondrocytes, whereas newborn mice knocked out of GABA(B)R1 (KO) showed a decreased body size and delayed calcification in hyoid bone and forelimb and hindlimb digits. Delayed calcification was also seen in cultured metatarsals from KO mice with a marked reduction of Indian hedgehog gene (Ihh) expression. Introduction of GABA(B)R1 led to synergistic promotion of the transcriptional activity of activating transcription factor-4 (ATF4) essential for normal chondrogenesis, in addition to facilitating ATF4-dependent Ihh ...
A A beta 42 abl acid phosphatase ACSM1 actin-general actin-alpha cardiac actin-alpha smooth muscle actin-muscle specific AE1/AE3 (cytokeratin) AF4 AGR2 AKT1 albumin alcian blue ALK alkaline phosphatase ALL1 alpha-1-antichymotrypsin alpha-1-antitrypsin alpha-fetoprotein alpha-lactalbumin AMACR AML1 androgen receptor APC API2-MALT1 Apolipoprotein D arginase1 ATM ...
On the basis of transmission electron microscopy observations in tumor cell lines, oncologists have made innumerous diagnostic and therapeutical progresses. Following this path, the UNICAMP immunopathologies laboratory established the NG97 cell line
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... periodic acid-Schiff per·i·od·ic acid-Schiff (pûrē-ŏdĭk) adj. Abbr. PAS Of, relating to, or being a reaction that tests for polysaccharides and related s
A study by light microscopy, using Leishman's stain alone or Leishman's stain followed by nigrosin, showed the presence of capsules on gonococci of two strains subcultured from subcutaneous chambers in guinea pigs. With the Alcian blue method of preparation for electron microscopy, gonococci of both strains recently grown in vivo showed densely stained capsules on some cells, while others in the same preparation showed only irregular masses of dense material on their surfaces with strands connecting adjacent bacteria. Treatment with antiserum, complement and conglutinin revealed irregular masses and strands of extracellular material with fixatives that did not contain Alcian blue.
The relationship between Ca++ and pinocytosis was investigated in Amoeba proteus. Pinocytosis was induced with 0.01% alcian blue, a large molecular weight dye which binds irreversibly to the cell surface. The time-course and intensity of pinocytosis was monitored by following the uptake of [3H]SUCROSE. When the cells are exposed to 0.01% alcian blue, there is an immediate uptake of sucrose. The cells take up integral of 10% of their initial volume during the time-course of pinocytosis. The duration of pinocytosis in the amoeba is integral of 50 min, with maximum sucrose uptake occurring 15 min after the induction of pinocytosis. The pinocytotic uptake of sucrose is reversibly blocked at 3 degrees C and a decrease in pH increases the uptake of sucrose by pinocytosis. The process of pinocytosis is also dependent upon the concentration of the inducer in the external medium. The association between Ca++ and pinocytosis in A. proteus was investigated initially by determining the effect of the ...
Recent studies described the phenotype of Fezl-deficient mice. Similarly to zebrafish, mammalian Fezl is expressed in the telencephalon and diencephalon (Hirata et al., 2004; Mutsuga et al., 2005). In the mouse telencephalon, Fezl is required for fate specification and axonal projections of cortico-spinal motor neurons, subplate cortical neurons and deep-layer pyramidal neurons (Chen et al., 2005a; Chen et al., 2005b; Hirata et al., 2004; Molyneaux et al., 2005). A close homolog of Fezl, denoted Fez, is expressed in the mouse hypothalamus in partially overlapping domains with Fezl and fez-fezl double deficient mouse displays defects in diencephalic subdivisions (Hirata et al., 2006a; Hirata et al., 2006b). A similar role for zebrafish Fezl in diencephalic patterning was also reported (Jeong et al., 2007).. The precise regulation of Otp, DA and IT by the tof m808 hypomorph (Figs 1 and 3) allows us to separate the role of Fezl in regional patterning from its more selective role in cell ...
It is widely believed that the increased cancer risk in UC is primarily acquired secondary to colonic inflammation.9,10 Although it is inherently logical that severely inflamed mucosa will confer an increased risk of neoplastic transformation, until recently there were few data to support this perception. However, a recent study from our centre has demonstrated a highly significant association between the severity of colorectal inflammation and neoplasia risk.5. There is a wide spectrum of colonoscopic appearances in patients with UC, all of which provide the colonoscopist with much information about that patients disease history. Patients may have endoscopic evidence of active inflammation (for example, friable mucosa or ulceration) although many patients will have no macroscopically active disease. Irrespective of acute changes, patients may have chronic endoscopic changes, such as scarring and post-inflammatory polyps, which signify previous significant inflammation but are unrelated to ...
Objective. Mature articular cartilage is vulnerable to injuries and disease processes that cause irreversible tissue damage because of its limited capacity for self-repair. Umbilical cord blood is a source of mesenchymal stem cells, which can give rise to cells of different lineages, including cartilage, bone, and fat. Cellular condensation is a required step in the initiation of mesenchymal chondrogenesis. We attempted to differentiate cells from umbilical cord blood into chondrocytes with insulin-like growth factor 1 (IGF-1) and transforming growth factor-beta 3 (TGF-beta 3). Methods. Cells were grown in high density micromass and monolayer culture systems and then evaluated for expression of type 11 collagen, aggrecan, and Sox9. Umbilical cord blood from 130 patients was harvested. Results. Expression of type II collagen, aggrecan, and Sox9 was detected after 14 days in TGF-beta 3- and IGF-1-stimulated cells in both types of culture (monolayer and micromass). On Day 21 in the micromass ...
Epithelial cells of different tissues or species diverge substantially in their culture requirements. Thus, in vivo-like culture of epithelial cells necessitates optimization of the entire culturing process including transport, isolation, medium composition and culture conditions. In the present study we established a new protocol for a differentiated cell culture system of the porcine cervical epithelium, based on easily accessible slaughterhouse material. The morphology and tested functional markers of our culture system are comparable to the native tissue as shown by histology, immunohistochemistry and alcian blue staining. The use of fibroblast-conditioned medium supported proliferation of cervical epithelial monolayers suggesting that stromal growth factors or cytokines released into the medium are required for cell growth in these epithelia. The supplementation of the conditioned medium with EGF further optimized proliferation and mitochondrial activity of the cervical epithelial cells. ...
The homogeneous, pink, mucinous alveolar exudate of bronchioloalveolar carcinoma is also PASD-positive. It is not foamy as in pneumocystis pneumonia or granular as in alveolar proteinosis. It stains with alcian blue, whereas the others do not. In this photo, not all of the alveoli are lined by tumor. The tumor cells (arrows) should not be mistaken for hyperplastic type II cells. Protein-rich pulmonary edema fluid can have a similar appearance but is PASD-negative.. Pulmonary alveolar proteinosis--PAS stain ...
Desmids a microscopic form of freshwater algae, exude mucilage, a transparent or almost transparent gelatinous substance, sometimes in copious quantities that can be easily seen under a microscope using normal transmitted light, for example in the desmid genus Hyalotheca which forms long filaments. In other genera it may be only faintly seen, if at all, and phase contrast or some method of staining will have to be used to show the extent of the mucilage. In my article (Micscape Dec.95) Some Observations on Freshwater Algae I referred to using Alcian blue in my experiments on Astrococcus the mucilage envelope of which did not not absorb the stain, although desmid mucilage always does. I also mentioned the use of diluted Indian ink the method shown here. A little diluted ink is placed on the slide next to the cover glass where it may spread under by capillary attraction or can be drawn under by removing water from the other side of the cover with blotting paper. ...
Z. R. Luo, R. L. Schultz, E. F. Whitter, L. Vollrath, Ultrastructural characterization of glial cells in the rat pineal gland with special reference to the pineal stalk, The Anatomical Record, 1984, 210, 4, ...
Sitokimia, gambaran morfologi se blas pada apus darah tepi atau sumsum tulang kadang-kadang tidak dapat membedakan LLA dari LMA. Pada LLA, pewarnaan sudan black dan mieloperoksidase adalah enzim sitoplasmik yang ditemukan pada granula primer dari prekursor granulositik, yang dapat dideteksi pada sel blas LMA. Sitokimia juga berguna untuk membedakan precursor B da B-ALL dan T-ALL. Pewarnaan fosfatase asam akan positif pada limfosit T yang ganas, sedangkan sel B dapat memberikan hasil yang positif pada pewarnaan periodic acid schiff (PAS). TdT yang diekspresikan oleh limfoblas dapat dideteksi dengan pewarnaan imunoperoksidase atau flow cytometry ...
18122-72-0 - XPMRNVZJQGDGFS-UHFFFAOYSA-I - Periodic acid (H5IO6), sodium salt (1:5) - Similar structures search, synonyms, formulas, resource links, and other chemical information.
HOPA = Hemalaun, Orange G, Phosphormolybdenic acid, Aniline blue Cell nuclei are stained with hemalaun or Weigerts iron-hematoxylin and then counterstained with a dye mixture of orange-phosphormolybden with aniline blue.. ...
Conjunctival cytology analysis (stained with periodic acid-Schiff, 40×).Notes: (A) refers to the staining before injection. (B) represents the staining 1 mon
Syndyoceras collyweston viperfish hyperneurotic polychromatophil corpusculum coenocentrum drapable safranin healthfulness scatologia geomyid aliveness raphidiid asthenobiosis [email protected] ...
Gram-positive coagulase-negative staphylococci have been implicated in breast prosthesis infection. Staphylococcus au re us, Staphylococcus epidermidis, and Staphylococcus hominis strains were examined for adhesion and proliferation morphology to polyurethane foam and smooth silicone rubber prosthetic biomaterials. Ruthenium red, alcian blue and ruthenium red-lysine in en bloc procedures were applied to optimally visualize the polysaccharide slime. Ruthenium red processing resulted in observation of additional outer material close to the cell wall, more than seen in the absence of cationic reagent. Alcian blue preserved fibrous or netlike strands or meshworks of material. Ruthenium red-lysine preserved considerable amounts of slime covering the cocci as well as extending between them and the prosthetic surface. Elongate strands of slime appeared to facilitate attachment to the substrate and projection of cells away from the substrate. Where this material was lacking, cell mass buildup was required for
A method for the simultaneous demonstration of lysozyme and mucins in 39 cases of gastric adenomas differentiated two intermediate cell types. The first was similar to a columnar cell comprising a single cell population which covered extensive areas of the adenomas. This cell type often showed supranuclear lysozyme reactivity and apical neutral mucins, sialomucins, and sulphomucins in variable amounts. The second cell type was found in 11 adenomas, located mainly in the fundal area. It seemed to be a transitional form between the goblet cell and the Paneth cell. This cell type was scattered among columnar cells, occasional Paneth-like cells, and small goblet cells. These two types of intermediate cells may be regarded as abnormally differentiated integral elements of gastric adenomas. They may be associated with the neck stem cells in the cytogenesis of gastric adenomas.. ...
Purpose: : During mouse retina maturation the final number of retinal ganglion cells (RGCs) is determined by highly regulated programmed cell death. Previous studies demonstrated that the immunoregulatory receptor programmed cell death-1 (PD-1) promotes developmental RGC death. To identify the functional signaling partner(s) for PD-1, we identified retinal expression of PD-1 ligands and examined the effect of PD-1 ligand expression on RGC number. We also explored the hypothesis that PD-1 signaling promotes development of functional visual circuitry. Methods: : Characterization of retinal and brain PD-L1 expression were examined by immunofluorescence on tissue sections. The contribution of PD-ligands to RGC number was examined in PD-ligand knockout mice. Retinal architecture was assessed by spectral domain optical coherence tomography and retinal function was analyzed by electroretinography in WT and PD-L1/L2 double deficient mice. Results: : PD-L1 expression is found throughout the neonatal ...
Background-Heart failure (HF) is a leading cause of mortality and morbidity, and the search for novel therapeutic approaches continues. In the monogenic disease mucopolysaccharidosis (MPS) VI, loss of function mutations in arylsulfatase B (ASB) leads to myocardial accumulation of chondroitin sulfate (CS) glycosaminoglycans (GAGs), manifesting as a myriad of cardiac symptoms. Here, we studied changes in myocardial CS in non-MPS failing hearts, and assessed its generic role in pathological cardiac remodeling. Methods-Healthy and diseased human and rat left ventricles were subjected to histological and immuno-staining methods to analyze for GAG distribution. GAGs were extracted and analyzed for quantitative and compositional changes using Alcian Blue assay and liquid chromatography mass spectrometry. Expression changes in 20 CS-related genes were studied in three primary human cardiac cell types and THP-1 derived macrophages under each of 9 in vitro stimulatory conditions. In two rat models of ...
Histologically, such abnormalities have been shown to be the sequelae of an error in metabolism of glycosaminoglycans within the cornea, in particular the proteoglycan keratan sulfate, resulting in its abnormal intra-and extracellular deposition [8]. These collections, staining with, amongst others, Alcian blue, form the basis of MCD classification, two major forms of which exist. This is based on measurements of the serum level of sulfated keratan sulfate (KS), the dominant glycosaminoglycan expressed in the adult cornea, as determined by antibodies directed against sulfated KS using enzyme-linked immunosorbent assay (ELISA) [9]. In Type I, accounting for the majority of patients, neither the cornea nor the serum contains normally sulfated KS, whereas in Type II there are low or normal amounts of antigenic KS in both the serum and the cornea 10. A subtype, IA, as yet distinct to Saudi Arabia and Germany, describes absent sulfated KS in the cornea and serum, but which is present in the ...
Histologically, such abnormalities have been shown to be the sequelae of an error in metabolism of glycosaminoglycans within the cornea, in particular the proteoglycan keratan sulfate, resulting in its abnormal intra-and extracellular deposition [8]. These collections, staining with, amongst others, Alcian blue, form the basis of MCD classification, two major forms of which exist. This is based on measurements of the serum level of sulfated keratan sulfate (KS), the dominant glycosaminoglycan expressed in the adult cornea, as determined by antibodies directed against sulfated KS using enzyme-linked immunosorbent assay (ELISA) [9]. In Type I, accounting for the majority of patients, neither the cornea nor the serum contains normally sulfated KS, whereas in Type II there are low or normal amounts of antigenic KS in both the serum and the cornea 10. A subtype, IA, as yet distinct to Saudi Arabia and Germany, describes absent sulfated KS in the cornea and serum, but which is present in the ...
Definition of deep fascia of neck. Provided by Stedmans medical dictionary and Drugs.com. Includes medical terms and definitions.
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Special stains: Histochemistry- Secretions within the epithelial cells and pseudoglandular spaces are PAS positive and diastase resistant, alcian blue and mucicarmine positive.. The stromal mucin secreted by the spindle cells are alcian blue positive but PAS negative.. Reticulin stain demonstrate the biphasic pattern of the tumor. Nests of plump rounded cells are highlighted by the reticulin stain.. Immunohistochemistry:. Synovial sarcoma demonstrates nuclear staining with TLE1 (transducin-like enhancer of split-1) in 95% of tumors and appears to be a very sensitive marker. TLE1 encodes for a transcriptional corepressor that is involved in epithelial and neuronal differentiation.. In Synovial sarcoma there is usually coexpression of mesenchymal (vimentin) and epithelial markers (cytokeratin and EMA).. The following immunomarkers are useful in the diagnosis:. - Cytokeratin (+). Only synovial sarcoma is positive with cytokeratins 7 and 19 , other soft tissue sarcomas including synovial sarcoma is ...
The objective of this study was to provide a new insight into the origin and lineage development of mucus-producing cells in the small intestinal crypt. For this, new experimental data were obtained from both crypt sections and whole mounts. Model simulation studies were undertaken to investigate which rules are most likely to govern the dynamic cellular development and goblet cell pedigree. We have measured the frequency of mucus-secreting goblet cells (using alcian blue and periodic acid Schiffs stains) at each cell position in the ileal murine crypt. These measurements, made on sections, overestimate the number of goblet cells because of the size and centripetal position of the stained cytoplasm. The correction factor for this overscoring has been measured to be 0.25 by two independent methods. The data suggest that there are about 12 functional goblet cells per crypt many of which retain an ability to divide. We have also determined the labelling index of the crypt goblet cells at each cell ...
The endothelial glycocalyx has been particularly difficult to characterize and understand in terms of its three-dimensional structure because of its fragility and instability [1]. Indeed, in addition to trauma, surgery, hyperglycemia, and sepsis, even subtle stimuli such as a plasma volume expansion can disrupt the glycocalyx structure [20, 23-27]. Disruption of glycocalyx exposes the endothelial cells to oxidative damage, and vascular hyperpermeability is observed in sepsis and chronic conditions such as diabetes and hypertension [5, 28]. There has been much effort to visualize endothelial glycocalyx using TEM and substitution of the original ruthenium red staining with lanthanum or alcian blue [7, 29, 30]. In the present study, we adopted lanthanum nitrate staining with a careful perfusion method that entailed (1) incision in the right atrial appendage to relieve pressure during perfusion fixation; (2) neck ligation for better perfusion of the heart, kidney, and liver; and (3) use of a ...
Perforating granuloma annulare, a secondary perforating disorder, was named by Owens and Freeman in 1971. The clinical diagnosis is definitely difficult when annular lesions are absent. Histopathologically, it exhibits transepidermal elimination of degenerating collagen, the so called "necrobiotic collagen". As the late A Bernard Ackerman used to stress, collagen is a fibre that can undergo degeneration but cannot become necrotic; only cells can undergo necrosis. There is a superficial area of the so called necrobiosis (better called degenerated collagen) and mucin accumulation surrounded by palisading histiocytes, situated beneath a perforation in the epidermis. The "necrobiotic material" and mucin are extruded via the perforation. Mucinous degeneration of collagen appears bluish or at least shows decrease in the degree of eosinophilic (pink) staining. Mucin can be seen more readily by staining with Alcian blue or colloidal iron.. ReplyDelete ...
The cutaneous focal mucinoses are a group of connective tissue disorders characterized by deposition of mucin found either focally or diffusely in the dermis. A 47-year-old woman presented with asymptomatic flesh-colored papules on the neck, inguinal area, intergluteal area, vulvar area, and extremities of 5 months duration. There was no history of preceding trauma or insect bites. The patient had undergone a subtotal thyroidectomy 21 years prior but had not used any thyroid medication before she was referred to our clinic. Thyroid ultrasonography was consistent with Hashimoto thyroiditis. During dermatologic examination, flesh-colored, well-defined, smooth papules that measured approximately 1.5 x 1 cm in size on the genital region, fingers, face, and scalp were seen. Histopathologic examination of a lesional biopsy revealed no abnormalities in the epidermis. Alcian blue staining showed that abundant deposits of dermal mucin had replaced collagen in the dermis ...
The cutaneous focal mucinoses are a group of connective tissue disorders characterized by deposition of mucin found either focally or diffusely in the dermis. A 47-year-old woman presented with asymptomatic flesh-colored papules on the neck, inguinal area, intergluteal area, vulvar area, and extremities of 5 months duration. There was no history of preceding trauma or insect bites. The patient had undergone a subtotal thyroidectomy 21 years prior but had not used any thyroid medication before she was referred to our clinic. Thyroid ultrasonography was consistent with Hashimoto thyroiditis. During dermatologic examination, flesh-colored, well-defined, smooth papules that measured approximately 1.5 x 1 cm in size on the genital region, fingers, face, and scalp were seen. Histopathologic examination of a lesional biopsy revealed no abnormalities in the epidermis. Alcian blue staining showed that abundant deposits of dermal mucin had replaced collagen in the dermis ...
This from OBrien and McCully, THE STUDY OF PLANT STRUCTURE: PRINCIPLES AND SELECTED METHODS (1981): 0.05%w/v water soluble aniline blue, CI No. 42755 (source: Polysciences Co.) in phospahte buffer, pH 8.5. Use freshly made solution or store in dark bottle in refrig. Discard when blue color changes to greenish. This is a flurochrome. Absorbance maximum is ca. 370 nm, peak emission is 509 nm (exciter BG12 combined with barrier 53 or barrrier 65 + 50 which will minimize chlorophyll autofluorescence). Dont know the original reference, but maybe this will do the trick. At 11:40 AM 6/26/98 -0700, you wrote: ,Would someone please post the recipe for the aniline blue stain used to ,stain pollen tubes in situ derived from Kho and Baer? Our library does not ,carry Euphytica, where it was published, and interlibrary loan has taken a ,month and still not come up with the paper. , ,Thanks. ,Peggy E. Pollak ,Department of Biological Sciences ,Northern Arizona University ,Flagstaff, AZ 86011-5640 , , , , ...
Purpose: : Melanoma-associated retinopathy (MAR), induced by cutaneous or uveal melanomas, results from the cross-reaction of tumor-directed autoantibodies with retinal antigens, which destroy retinal tissue. MAR ultrastructural descriptions and autoantibody localization are lacking. We present for the first time ocular ultrastructure and the retinal targets of the autoantibodies of MAR. Methods: : A pair of autopsied eyes of an 80 year-old male with metastatic cutaneous melanoma and positive retinal autoantibodies was examined with histopathology including transmission electron microscopy, and immunohistochemistry (Calbindin, calcium binding proteins; PKCalpha, protein kinas C; and TRPM1, a mGluR6-coupled cation channel in ON bipolar cells) using avidin-biotin-complex immunohistochemistry. Results: : The outer plexiform layer (OPL) was the most affected retinal tissue in both eyes; it showed thinning and atrophy, which extended to both the outer (ONL) and inner (INL) nuclear layers, resulting ...
I know that the reaction proceeds by forming a cyclic diester of periodic acid followed by cleavage of $\ce{C-C}$ bond and formation of $\ce{C=O}$ bond. But how do we compare the rates of the given compounds?. My first thought was to think $\ce{C-C}$ cleavage as formation of a pseudo carbocation which would be stabilised by +I effect of the alkyl groups and also the hyperconjugation, but I dont think it is right.. ...
Remove the white tape from the gel. Notice the horizontal line under the white tape? Later you will run the gel until the blue stain frontier reaches this line. Flush the wells with water. Mark the bottom of each well with a sharpie. This is a useful guide for your pipette when you insert your samples. ...
I cut them in 50 microliter volume with HF Restriction enzymes (I do not overdigest), AP the vector with antartic phosphatase, and then dna purify with the quiagen columns. I do admit I exposed the DNA to UV light, but less than 1 minute, run a little aliquot to check that it was a single band and I did, used 10 ng/microliter concentration in a 10microliter volume ligation (ratio 1:1/1:2/1:3), ligation 4C overnight, DH5 alpha/TOP10/XL2 blue cells, have few colonies with the backbone AP and 4x more in the ligation plates (after several trials decided to go with DH5alfa which gave me more colonies than the others ...
The treacherous season has arrived and Im feeling the onset of the descent; the path upon which I set foot five years ago this very weekend. Mild concern that transitioned into confusion, then snowballed into sheer panic and eventually converged on December 15 with a diagnosis. A sentence. A death sentence that wouldnt register at the time. It couldnt... then. Determinations from the white coats who had peered into microscopes in a cold lab, scrutinizing slides holding slices of tissue - small round blue cells with their scant neoplasm, a translocation between chromosomes 11 and 22. What ...
Restructuring Genetics with BLUE GENE A log by jaymdubbs What is Blue GENE ? Serving Size: 5 Tabs (5950mg) Servings Per Container: 30 Controlled
As scientists take on the challenge of aging, they look at decay and the body. But theres a cultural side to getting older that we shouldnt push aside.
Could you please identify a pill I found at my fiances house. Im concerned because of a depression hes going through. Its blue and is shaped like
The efficient removal of proteoglycans, such as decorin, from the hide when processing it to leather by traditional means is generally acceptable and beneficial for leather quality, especially for softness and flexibility. A patented waterless or acetone dehydration method that can generate a product similar to leather called Dried Collagenous Biomaterial (known as BCD) was developed but has no effect on decorin removal efficiency. The Alcian Blue colorimetric technique was used to assay the sulfated glycosaminoglycan (sGAG) portion of decorin. The corresponding residual decorin content was correlated to the mechanical properties of the BCD samples and was comparable to the control leather made traditionally. The waterless dehydration and instantaneous chrome tanning process is a good eco-friendly alternative to transforming hides to leather because no additional effects were observed after examination using NIR spectroscopy and additional chemometric analysis.
The efficient removal of proteoglycans, such as decorin, from the hide when processing it to leather by traditional means is generally acceptable and beneficial for leather quality, especially for softness and flexibility. A patented waterless or acetone dehydration method that can generate a product similar to leather called Dried Collagenous Biomaterial (known as BCD) was developed but has no effect on decorin removal efficiency. The Alcian Blue colorimetric technique was used to assay the sulfated glycosaminoglycan (sGAG) portion of decorin. The corresponding residual decorin content was correlated to the mechanical properties of the BCD samples and was comparable to the control leather made traditionally. The waterless dehydration and instantaneous chrome tanning process is a good eco-friendly alternative to transforming hides to leather because no additional effects were observed after examination using NIR spectroscopy and additional chemometric analysis.
A method is given for incorporating diverse varieties of intercalants or templates directly during hydrothermal synthesis of clays such as hectorite or montmorillonite-type layer-silicate clays. For a hectorite layer-silicate clay, refluxing a gel of silica sol, magnesium hydroxide sol and LiF for 2 days with an organic or organometallic intercalant or template results in crystalline products containing either (a) organic dye molecules such as ethyl violet and methyl green, (b) dye molecules such as alcian blue based on a Cu(II)-phthalocyannine complex, or (c) transition metal complexes such as Ru(II)phenanthroline and Co(III)sepulchrate or (d) water-soluble porphyrins and metalloporphyrins. Montmorillonite-type clays are made by the method taught by US patent No. 3,887,454 issued to Hickson, June 13, 1975; however, a variety of intercalants or templates may be introduced. The intercalants or templates should have water-solubility, positive charge, and thermal stability under moderately basic (pH 9-10)
Medical term deep fascia 深筋膜; 深肌膜 and its variants in English, Simplified Chinese, Traditional Chinese and Pinyin with audio pronunciation. 规范医学术语词汇
Each dye molecule has at least two functional chemical groupings. The auxochrome ionizes and gives the molecule the ability to react with the substrate, while the unsaturated chromophore absorbs specific wavelengths of light. The color of the solution obtained is that of the unabsorbed (transmitted) light. To be a dye, the molecule must have both auxochrome and chromophore groups. The auxochrome is usually an ionized carboxyl, hydroxyl, or pentavalent nitrogen group. The chromophore may have unsaturated nitrogen bonds such as azo (-N=N-) indamine (-N=), nitroso (-N=O) or nitro (O-N=O), groups; or unsaturated carbon to carbon, carbon to oxygen, or carbon to sulfur bonds, such as ethenyl (C=C), carbonyl (C=O), C=S, or the quinoid ring (= = =). Cationic dyes will react with substrate groups that ionize to produce a negative charge, such as carboxyl, phenolic, or sulfhydryl groups. Anionic dyes will react with substrate groups which ionize to produce positive charges, such as the ammonium ion. Any ...
Transmembrane potential measurement methods using cationic dyes, and anionic dyes are provided. Compositions of the cationic and anionic dyes and microfluidic systems which include the dyes and membranes are provided in conjunction with processing elements for transmembrane potential measurements.
PAS reaction) a test for the presence of glycoproteins, polysaccharides, certain mucopolysaccharides, glycolipids, and certain fatty acids in tissue sections. The tissue is treated with periodic acid, followed by Schiffs reagent. A positive reaction is the development of a red or magenta coloration. ...
Assayed, human whole blood control for reticulocyte counting using Beckman Coulter® VCS instruments and New Methylene Blue stain; level 2 of 3 (4 x 4 mL)
Ive just returned from Copenhagen, Denmark, where I attended the 8th International Colloquium on Paratuberculosis (at considerable personal expense: Copenhagen is EXPENSIVE!) Current thinking among researchers in the field is that MAP itself is mostly not the culprit in the tissue damage. MAPs role is to initiate an inflammatory response in the bowel, through some unknown process/antigen/inflammatory-pathway. This immune response results in inflammation of bowel tissue, whereby the cells of the intestine physically separate, to allow immune cells, primarily macrophages and CD4 + CD8 T-cells, to reach the site of infection. This leads to "leaky gut" syndrome , which permits the contents of the bowel (most accurately described as being similar to the contents of a sewer), to leak through the bowel wall, resulting in a *massive* inflammatory response against the many microbes present in the fecal stream. Continue reading →. ...
Ive just returned from Copenhagen, Denmark, where I attended the 8th International Colloquium on Paratuberculosis (at considerable personal expense: Copenhagen is EXPENSIVE!) Current thinking among researchers in the field is that MAP itself is mostly not the culprit in the tissue damage. MAPs role is to initiate an inflammatory response in the bowel, through some unknown process/antigen/inflammatory-pathway. This immune response results in inflammation of bowel tissue, whereby the cells of the intestine physically separate, to allow immune cells, primarily macrophages and CD4 + CD8 T-cells, to reach the site of infection. This leads to "leaky gut" syndrome , which permits the contents of the bowel (most accurately described as being similar to the contents of a sewer), to leak through the bowel wall, resulting in a *massive* inflammatory response against the many microbes present in the fecal stream. Continue reading →. ...
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Safranin is used as a dye for textiles and as a stain for various scientific procedures. Scientific procedures that involve safranin include Gram counterstaining, endospore counterstaining, granule...
Question by addicted: i want to stop smoking crack cocaine. I dont no how. dont know how to even start try. please help.? Best answer: Answer by lanceGo to
A 15-YEAR-OLD boy is to face trial after he pleaded not guilty to hampering a Garda drug search by swallowing a bag of crack cocaine.
Fully tested and working Micromass GCT with EI source, Agilent 6890N GC with split/splitless, CTC HTS modified/Combi Pal with Liquid injection only(l
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引用Abcams Anti-CD68抗体[PG-M1] (ab783)的参考文献列表。为您列举引用本产品的发表文章,并提供信息包括论文文献数据库中的检索编号以便您搜寻文章
Cartilage tissue regeneration often presents a challenging clinical situation. Recently, it has been shown that Periodontal Ligament Stem Cells (PDLSCs) possess high chondrogenic differentiation capacity. In this study, we developed a stem cell delivery system based on alginate/hyaluronic acid (HA) loaded with TGF-β1 ligand, encapsulating PDLSCs; and investigated the chondrogenic differentiation of encapsulated cells in alginate/HA hydrogel microspheres in vitro and in vivo. The results showed that PDLSCs, as well as human bone marrow mesenchymal stem cells (hBMMSCs), as the positive control, were stained positive for both toluidine blue and alcian blue staining, while exhibiting high levels of gene expression related to chondrogenesis (Col II, Aggrecan and Sox-9), as assessed via qPCR ...
Chronic Rhinosinusitis (CRS) is a common inflammatory condition of the upper respiratory tract lasting more than 8 to 12 weeks. Major symptoms include facial congestion/fullness, facial pain/pressure, nasal obstruction/blockage, purulent nasal drainage, and reduction or loss of smell. The diagnosis must include two major symptoms and either endoscopic evidence of polyps, edema or mucopurulent discharge from the middle meatus and/or CT changes in the mucosa of sinuses or ostiomeatal complexes.. CRS has an estimated prevalence of 5% in the Canadian population, and up to 16% in some adult populations in the United States. Sinusitis is associated with a major societal health care burden, costing billions of dollars a year in North America. The medical treatment of CRS includes topical saline and corticosteroid sprays, systemic steroids and antimicrobials. Specifically, sinonasal saline irrigation is a safe, nonpharmacologic treatment, and an important and efficacious component in the management of ...
The Gram-negative bacterium Flavobacterium psychrophilum, which is the causative agent of rainbow trout fry syndrome (RTFS), and infectious pancreatic necrosis virus (IPNV), the causative agent of infectious pancreatic necrosis (IPN), are both highly pathogenic for rainbow trout fry. Several persistent cases of RTFS have been observed concomitant with IPNV. Cultivation alone might not be sufficient for evaluation of the disease situation as both pathogens can be cultivated from fish that do not show any clinical signs of disease. In such cases it may be difficult to decide which pathogen should be considered the primary cause of the mortality observed. Further, it may be difficult to cultivate the bacterium in later stages of the disease or from dead fish that have been transported without cooling. In the case of (suspected) double infections it is therefore suggested that immunohistochemistry be included as a supplementary diagnostic tool, allowing correlation of the presence of either ...

Alcian blue stain - WikipediaAlcian blue stain - Wikipedia

Alcian blue or alcian blue (/ˈælʃən/) is any member of a family of polyvalent basic dyes, of which the Alcian blue 8G (also ... Solid Alcian blue is a combustible powder and should never be handled close to heat or a naked flame. Heating Alcian blue ... In addition to its use as a dye or stain Alcian blue also finds other material science uses. Alcian blue has been used as an ... Scott also states that Alcian green was merely a mixture of Alcian blue and Alcian yellow and not a single compound, which is ...
more infohttps://en.wikipedia.org/wiki/Alcian_blue_stain

Re: [Histonet] alcian blue pHRe: [Histonet] alcian blue pH

... From:. Geoff McAuliffe Hi Tom: Alcian Blue will stain the glycosaminoglycans in the ... Still, the traditional method is 1% Alcian Blue in 3% acetic acid for a pH of 2.5. At 2.5 both sulfated and carboxylated GAGs ... with alcian blue. Interest in cellular morphology of nucleus pulposus, transition and annulus cells. > >What pH of the stain ... You might try 0.05% Toluidine blue in dist water with a pH of about 4.0 for 10-15 minutes, dist water rinse and dehydration in ...
more infohttp://www.histosearch.com/histonet/Aug04A/Re.HistonetalcianbluepH.html

The Crosstalk Between Transforming Growth Factor-β1 and Delta Like-1 Mediates Early Chondrogenesis During Embryonic...The Crosstalk Between Transforming Growth Factor-β1 and Delta Like-1 Mediates Early Chondrogenesis During Embryonic...

A): Pellets were stained for proteoglycans with Toluidine Blue and Alcian Blue and for type II collagen by immunohistochemistry ... Proteoglycans were visualized by Toluidine Blue and Alcian Blue stainings. Sox9, type II collagen, and Dlk1/FA1 depositions ... more intense staining for proteoglycans by Toluidine Blue and Alcian Blue and for type II collagen, and significant ... C): Limb bud cultures were analyzed by Alcian Blue staining and FA1 immunohistochemistry during differentiation. (D): Secretion ...
more infohttp://onlinelibrary.wiley.com/doi/10.1002/stem.792/full

Alcian Blue (band) - WikipediaAlcian Blue (band) - Wikipedia

Alcian Blue was a shoegaze and new wave-influenced band operating out of the Washington, DC suburb of Takoma Park from 1998 to ... "Alcian Blue - Alcian Blue - Songs, Reviews, Credits - AllMusic". AllMusic. Retrieved 2016-08-09. http://www.safraninsound.com/ ... Slow Colorless Stare CD (2001, Safranin Sound) Alcian Blue CD (2006, Elephant Stone) Angelica Take Me Down EP (2003, Safranin ...
more infohttps://en.wikipedia.org/wiki/Alcian_Blue_(band)

alcian blue Protocols and Video...'alcian blue' Protocols and Video...

... alcian blue include Using Unfixed, Frozen Tissues to Study Natural Mucin Distribution, Adult Zebrafish Injury Models to ... Alcian Blue: A copper-containing dye used as a gelling agent for lubricants, for staining of bacteria and for the dyeing of ...
more infohttps://www.jove.com/keyword/alcian+blue

Alcian Blue | National DiagnosticsAlcian Blue | National Diagnostics

Alcian Blue in conjunction with PAS (Mowry, 1956) is commonly used to differentiate acid and neutral mucopolysaccharides. It ...
more infohttps://www.nationaldiagnostics.com/histology/product/alcian-blue

Alcian Blue 8GX, certified, ACROS Organics™
 | Fisher ScientificAlcian Blue 8GX, certified, ACROS Organics™ | Fisher Scientific

Ingrain Blue 1. SMILES. CN(C)C(=[N+](C)C)SCC1=CC2=C(C=C1)C3=NC4=C5C=C(C=CC5=C([N-]4)N=C6C7=C(C=CC(=C7)CSC(=[N+](C)C)N(C)C)C(=N6 ...
more infohttps://www.fishersci.ca/shop/products/alcian-blue-8gx-certified-acros-organics-3/p-2690982

Alcian Blue Staining Technique for Tracing the Governing Vessel (GV) Meridian in Rat | SpringerLinkAlcian Blue Staining Technique for Tracing the Governing Vessel (GV) Meridian in Rat | SpringerLink

After injection of Alcian blue at LSP (lumbar sacral point) of the GV meridian, we observed a blue line at the GV meridian ... Acupuncture meridian Alcian blue Bonghan duct system Lumbar sacral point This is a preview of subscription content, log in to ... 2007) Alcian Blue Staining Technique for Tracing the Governing Vessel (GV) Meridian in Rat. In: Magjarevic R., Nagel J.H. (eds ... Only very recently we found a staining dye, Alcian blue that stains the acupuncture meridian and were able to trace the ...
more infohttps://link.springer.com/chapter/10.1007%2F978-3-540-36841-0_904

Leica Alcian Blue Special Stain Kit  - Product: Leica BiosystemsLeica Alcian Blue Special Stain Kit - Product: Leica Biosystems

Leica Alcian Blue Special Stain Kit Consumables Leica Alcian Blue Special Stain Kit *Product ... Alcian Blue is normally prepared at an acidic pH of 2.5 and is used to identify acid mucopolysaccharides and acetic mucins. ...
more infohttps://www.leicabiosystems.com/histology-consumables/special-stain-kits/products/leica-alcian-blue-special-stain-kit/

Alcian Blue - Alizarin Red Staining of Mouse SkeletonAlcian Blue - Alizarin Red Staining of Mouse Skeleton

Our lab has used the Alcian blue - Alizarin red staining method with certain modifications to characterize skeleton deformities ... staining the cartilage is done with 0.02% alcian blue (as in your protocol) or 0.2% alcian blue (as in MBC paper)?. Thanks ... Alcian blue 8GX (prepared in ethanol/ glacial acetic acid, 7:3) for 1 to 2 days.. Note: Cartilage tissues will be stained blue ... Zhang, P. (2012). Alcian Blue - Alizarin Red Staining of Mouse Skeleton. Bio-protocol 2(8): e162. DOI: 10.21769/BioProtoc.162. ...
more infohttps://bio-protocol.org/e162

Histonet] Alcian Blue digested w Hyaluronidase - any alternatives?Histonet] Alcian Blue digested w Hyaluronidase - any alternatives?

... Hobbs, Carl carl.hobbs at kcl.ac.uk Thu Aug 23 13:32:13 CDT ... Perhaps Alcian blue at pH3 ( all acidic mucins) on one slide/section and Ab pH1 ( highly sulphated mucins only) on a sequential ... Previous message (by thread): [Histonet] Alcian Blue digested w Hyaluronidase - any alternatives? ... Next message (by thread): [Histonet] Alcian Blue digested w Hyaluronidase - any alternatives? ...
more infohttp://lists.utsouthwestern.edu/pipermail/histonet/2018-August/079160.html

Orcein-alcian blue staining: a new technique for demonstrating acid mucins in gastrointestinal epithelium. | Journal of...Orcein-alcian blue staining: a new technique for demonstrating acid mucins in gastrointestinal epithelium. | Journal of...

Orcein-alcian blue is a safer, cheaper, and quicker method than high iron diamine-alcian blue which can be safely introduced ... Orcein-alcian blue staining, a new method for the simultaneous demonstration of sulphated and sialomucins in gastrointestinal ... Orcein-alcian blue staining: a new technique for demonstrating acid mucins in gastrointestinal epithelium. ... Orcein-alcian blue staining: a new technique for demonstrating acid mucins in gastrointestinal epithelium. ...
more infohttp://jcp.bmj.com/content/42/8/881

Alcian Blue on Red Blood Cell Membrane - How is Alcian Blue on Red Blood Cell Membrane abbreviated?Alcian Blue on Red Blood Cell Membrane - How is Alcian Blue on Red Blood Cell Membrane abbreviated?

Looking for abbreviations of ABRBC? It is Alcian Blue on Red Blood Cell Membrane. Alcian Blue on Red Blood Cell Membrane listed ... Alcian Blue on Red Blood Cell Membrane - How is Alcian Blue on Red Blood Cell Membrane abbreviated? https://acronyms. ... a href=https://acronyms.thefreedictionary.com/Alcian+Blue+on+Red+Blood+Cell+Membrane,ABRBC,/a,. *Facebook ... redirected from Alcian Blue on Red Blood Cell Membrane) Acronym. Definition. ABRBC. Alcian Blue on Red Blood Cell Membrane. ...
more infohttps://acronyms.thefreedictionary.com/Alcian+Blue+on+Red+Blood+Cell+Membrane

Alcian Blue Pathology Outlines | The ScientificAlcian Blue Pathology Outlines | The Scientific

Alcian Blue Pathology Outlines. Posted byadmin April 27, 2019. Thomas Edison Film Projector Each film was approximately 17 ... Alcian blue stain showed pools of mucin in the.. From the Departments of *Rheumatology and Clinical Immunology and †Trauma and ... Then we made thinly sliced dorsal sections of removed trachea and stained them with hematoxylin and eosin (HE) or alcian blue ( ... Importantly, at the age of 6 months, around 40% of CPK mice displayed cystic lesions classified as alcian blue/PAS-positive ...
more infohttps://www.profissionaldebermuda.com/alcian-blue-pathology-outlines/

Best Labs and Diagnostic Centers for Alcian Blue Stain Urine in Bhubaneswar | 1mgBest Labs and Diagnostic Centers for Alcian Blue Stain Urine in Bhubaneswar | 1mg

Find the best Labs and Diagnostic Centers for Alcian Blue Stain Urine Online in Bhubaneswar. View its pre-requirement, prices ...
more infohttps://www.1mg.com/labs/bhubaneswar/alcian-blue-stain-urine-1933-p

Hito Alcian Blue pH 2.5 OptimStain™ Kit | Hitobiotec Corp.Hito Alcian Blue pH 2.5 OptimStain™ Kit | Hitobiotec Corp.

Hito Alcian Blue pH-2.5 OptimStain™ Kit is formulated for the staining of acid mucins, sulfated / carboxylated acid ... Hito Alcian Blue pH-2.5 OptimStain™ Kit has been tested extensively on the tissues from several species of animals and it is a ... Hito Alcian Blue pH-2.5 OptimStain™ Kit has been tested extensively on the tissues from several species of animals and it is a ... Hito Alcian Blue pH-2.5 OptimStain™ Kit is designed in a ready-to-use format and offers high quality, rapid and reproducible ...
more infohttps://www.hitobiotec.com/hito-alcian-blue-ph-25-optimstain-kit.html

ALCIAN BLUE pH 2.5, Multi-Tissue Control Histology Slides - Newcomer SupplyALCIAN BLUE pH 2.5, Multi-Tissue Control Histology Slides - Newcomer Supply

Newcomer Supply Alcian Blue pH 2.5, Multi-Tissue Control Slides use a combination of tissue sources for the positive ... Alcian Blue 1%, pH 2.5 Stain Kit Alcian Blue 1%, pH 2.5 Stain Kit. 250ml kit. 9102A. $153.90. ... Alcian Blue Stain 1%, pH 2.5 Aqueous Alcian Blue Stain 1%, pH 2.5 Aqueous. 250 ml. 1003A. $69.80. ... With Alcian Blue 1%, pH 2.5 Stain Kit: Part 9102A/B. Individual Stain Solution. ...
more infohttp://www.newcomersupply.com/product/alcian-blue-ph-25-multi-tissue

Posterior gut (Alcian Blue-PAS) from fish fed (A) Fish  | Open-iPosterior gut (Alcian Blue-PAS) from fish fed (A) Fish | Open-i

Alcian Blue-PAS) from fish fed (A) Fish oil-based diet (FO), (B) FO- and MOS-based diet (FOMOS), (C) soy bean meal-based diet ( ... Figure 1: Posterior gut (Alcian Blue-PAS) from fish fed (A) Fish oil-based diet (FO), (B) FO- and MOS-based diet (FOMOS), (C) ... Figure 1: Posterior gut (Alcian Blue-PAS) from fish fed (A) Fish oil-based diet (FO), (B) FO- and MOS-based diet (FOMOS), (C) ...
more infohttps://openi.nlm.nih.gov/detailedresult.php?img=PMC4525062_fimmu-06-00397-g001&req=4

ALCIAN BLUE 8 GX (For Molecular Biology)   (CAS No.75881-23-1) Laboratory Chemicals Exporters, Lab Chemical Suppliers, Chemical...ALCIAN BLUE 8 GX (For Molecular Biology) (CAS No.75881-23-1) Laboratory Chemicals Exporters, Lab Chemical Suppliers, Chemical...

ALCIAN BLUE 8 GX (For Molecular Biology) Chemical, We are supplier and exporter of Laboratory Chemical like ALCIAN BLUE 8 GX ( ... visit the page for COA information of ALCIAN BLUE 8 GX (For Molecular Biology) or (CAS No.75881-23-1) ... Certificate of Analysis of ALCIAN BLUE 8 GX (For Molecular Biology). Product Name: molecular formula: Weight: Art NO: Batch No: ... COA information of ALCIAN BLUE 8 GX (For Molecular Biology), Alpha Chemika - Exporter of Laboratory Chemical, Lab Chemicals, ...
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Frontiers | Phosphomannosylation and the Functional Analysis of the Extended Candida albicans MNN4-Like Gene Family |...Frontiers | Phosphomannosylation and the Functional Analysis of the Extended Candida albicans MNN4-Like Gene Family |...

Alcian Blue Binding Assays. The phosphomannan content was determined by measuring the binding of the cationic dye Alcian Blue ... Alcian Blue and found that none of the complemented transformants were capable of restoring wild-type levels of Alcian Blue ... Aliquots of 1 mL were pelleted, and cells suspended in 1 mL of 30 μg/mL Alcian Blue (in 0.02 M HCl) and incubated at room ... Complementation of this strain with MNN41 did not increase Alcian Blue binding, but complementation with either MNN42 or MNN47 ...
more infohttps://www.frontiersin.org/articles/10.3389/fmicb.2017.02156/full

A zebrafish model of lethal congenital contracture syndrome 1 reveals Gle1 function in spinal neural precursor survival and...A zebrafish model of lethal congenital contracture syndrome 1 reveals Gle1 function in spinal neural precursor survival and...

Bleached embryos were washed twice in PBS and incubated in Alcian Blue solution (0.1% Alcian Blue, 70% ethanol, 1% HCl) ... Alcian Blue staining. Larvae at 5 dpf were fixed in 4% paraformaldehyde in PBS at 4°C overnight, washed in PBS, and bleached in ... Cell death is noted in the head (F, arrow) and spinal cord (F, inset). (G,H) Alcian Blue-stained head cartilages of 5-dpf wild- ... Embryos subjected to whole-mount in situ hybridization or Alcian Blue staining were mounted in a bridged slide containing one ...
more infohttp://dev.biologists.org/content/139/7/1316.long

Histology Lab: ALCIAN BLUE TECHNIQUEHistology Lab: ALCIAN BLUE TECHNIQUE

Alcian Blue pH 3.1. *Alcian Blue 1g. *0.5% Acetic Acid 100ml. *Alcian Blue pH 2.5. *Alcian Blue 1g. *3% Acetic Acid 100ml. * ... Alcian Blue pH 1.0. *Alcian Blue 1g. *0.1M Hydrochloric Acid 100ml. *Alcian Blue pH 0.2. * Alcian Blue 1g. * 10% Sulphuric Acid ... Strongly sulphated mucins - use pH 0.2 Alcian Blue. *Weakly sulphated mucins - use pH 2.5 or pH 1.0 Alcian Blue. *When using ... ALCIAN BLUE TECHNIQUE SECTIONS. 4micron Paraffin wax sections. SOLUTIONS. * ...
more infohttp://www.protocol-online.org/cgi-bin/prot/view_cache.cgi?ID=595

Disc1 regulates foxd3 and sox10 expression, affecting neural crest migration and differentiation | DevelopmentDisc1 regulates foxd3 and sox10 expression, affecting neural crest migration and differentiation | Development

Alcian Blue analysis. Larvae at 5 days post-fertilization (dpf) were stained with Alcian Blue as described (Kimmel et al., 1998 ... Alcian Blue staining of the cartilaginous craniofacial skeleton, a CNC derivative, revealed abnormal development in a high ... 2C-H). Disc1 morphants fell into three classes based on their Alcian Blue staining: (1) normal; (2) mild malformations of the ... Q) foxd3 in situ hybridization and Alcian Blue staining of craniofacial cartilage was done on Disc1 morphants and mismatch ...
more infohttp://dev.biologists.org/content/136/15/2623

Effective CRISPR/Cas9-based nucleotide editing in zebrafish to model human genetic cardiovascular disorders | Disease Models &...Effective CRISPR/Cas9-based nucleotide editing in zebrafish to model human genetic cardiovascular disorders | Disease Models &...

Cartilage staining (Alcian Blue). Zebrafish larvae at 5 dpf were incubated overnight at 4°C in fixative solution (76% ethanol; ... Next to cardiovascular anomalies, kcnj8 V65M mutants were assessed for craniofacial phenotypes by Alcian Blue staining at 5 dpf ... 20% acetic acid; 4% formaldehyde supplemented with 15mg Alcian Blue). Subsequently, larvae were washed in 70-100% ethanol, ...
more infohttp://dmm.biologists.org/content/11/10/dmm035469
  • In addition to its wide use as a stain Alcian blue has also been used in other diverse applications e.g. gelling agent for lubricating fluids, modifiers for electrodes, charged coating agents etc. (wikipedia.org)
  • Alcian blue or alcian blue (/ˈælʃən/) is any member of a family of polyvalent basic dyes, of which the Alcian blue 8G (also called Ingrain blue 1, and C.I. 74240, formerly called Alcian blue 8GX from the name of a batch of an ICI product) has been historically the most common and the most reliable member. (wikipedia.org)
  • In attempt to answer what was the importance of discovering an alternative method of manufacturing this compound, a company (Anatech Ltd, USA) that remanufactured Alcian blue says: "Alcian blue is highly selective for the tissue substances (given the proper solution pH), and forms insoluble complexes that withstand harsh subsequent treatment (like PAS) without destaining. (wikipedia.org)
  • After injection of Alcian blue at LSP (lumbar sacral point) of the GV meridian, we observed a blue line at the GV meridian above loose connective tissue. (springer.com)
  • Biochemists had used it to assay acid polysaccharides in urine since the 1960s for diagnosis of diseases like mucopolysaccharidosis but from 1970's, partly due to lack of availability of Alcian and partly due to length and tediousness of the procedure, alternative methods had to be developed e.g. (wikipedia.org)
  • The Monstral blue found to coat the inside of copper vessels used to process phthalic acid derivatives had led to the discovery of Phthalocyanine in 1907. (wikipedia.org)
  • They were stained with orcein for four hours, differentiated for a few seconds in acid alcohol, and then counterstained with alcian blue for half to one minute. (bmj.com)
  • To determine disease severity, adjacent sections stained with periodic acid-Schiff (PAS)/Alcian blue were examined histologically. (profissionaldebermuda.com)
  • The samples were then stained with different dyes under standard conditions for hematoxylin-eosin techniques, Masson's trichrome, orcein, and periodic acid-Schiff Alcian blue to a pH of 2.5. (profissionaldebermuda.com)
  • Use of alcian blue has historically been a popular staining method in histology especially for light microscopy in paraffin embedded sections and in semithin resin sections. (wikipedia.org)
  • Alcian blue can be used to quantitate acidic glycans both in microspectrophotometric quantitation in solution or for staining glycoproteins in polyacrylamide gels or on western blots. (wikipedia.org)
  • Hito Alcian Blue pH-2.5 OptimStain™ Kit has been tested extensively on the tissues from several species of animals and it is a simple solution for your research. (hitobiotec.com)
  • You might try 0.05% Toluidine blue in dist water with a pH of about 4.0 for 10-15 minutes, dist water rinse and dehydration in 3 changes of actone followed by clearing in fresh xylene. (histosearch.com)
  • I would like to stain some intervertebral disc x-sections (rat, decalcified) with alcian blue. (histosearch.com)
  • Then we made thinly sliced dorsal sections of removed trachea and stained them with hematoxylin and eosin (HE) or alcian blue (AB) for light microscopic examination. (profissionaldebermuda.com)
  • Hito Alcian Blue pH-2.5 OptimStain™ Kit is designed in a ready-to-use format and offers high quality, rapid and reproducible staining. (hitobiotec.com)
  • After spending 3 man-years of effort (which would have been unnecessary had the industry released the results) in 1972 he published the structure of Alcian blue and was able to get ICI to confirm it in 1973, incidentally in the same time that ICI also had just stopped producing it. (wikipedia.org)
  • Prussian Blue or Perls' reaction is used to demonstrate ferric iron and ferritin. (polysciences.com)