Alcian Blue: A copper-containing dye used as a gelling agent for lubricants, for staining of bacteria and for the dyeing of histiocytes and fibroblasts in vivo.Periodic Acid-Schiff Reaction: A histochemical technique for staining carbohydrates. It is based on PERIODIC ACID oxidation of a substance containing adjacent hydroxyl groups. The resulting aldehydes react with Schiff reagent to form a colored product.Glycosaminoglycans: Heteropolysaccharides which contain an N-acetylated hexosamine in a characteristic repeating disaccharide unit. The repeating structure of each disaccharide involves alternate 1,4- and 1,3-linkages consisting of either N-acetylglucosamine or N-acetylgalactosamine.Staining and Labeling: The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.Coloring Agents: Chemicals and substances that impart color including soluble dyes and insoluble pigments. They are used in INKS; PAINTS; and as INDICATORS AND REAGENTS.Histocytochemistry: Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.Tolonium Chloride: A phenothiazine that has been used as a hemostatic, a biological stain, and a dye for wool and silk. Tolonium chloride has also been used as a diagnostic aid for oral and gastric neoplasms and in the identification of the parathyroid gland in thyroid surgery.Sialomucins: A subcategory of mucins that contain SIALIC ACID.Metaplasia: A condition in which there is a change of one adult cell type to another similar adult cell type.Mucins: High molecular weight mucoproteins that protect the surface of EPITHELIAL CELLS by providing a barrier to particulate matter and microorganisms. Membrane-anchored mucins may have additional roles concerned with protein interactions at the cell surface.Mucus: The viscous secretion of mucous membranes. It contains mucin, white blood cells, water, inorganic salts, and exfoliated cells.Chondrogenesis: The formation of cartilage. This process is directed by CHONDROCYTES which continually divide and lay down matrix during development. It is sometimes a precursor to OSTEOGENESIS.Pyronine: Xanthene dye used as a bacterial and biological stain. Synonyms: Pyronin; Pyronine G; Pyronine Y. Use also for Pyronine B. which is diethyl-rather than dimethylamino-.Uronic Acids: Acids derived from monosaccharides by the oxidation of the terminal (-CH2OH) group farthest removed from the carbonyl group to a (-COOH) group. (From Stedmans, 26th ed)Cartilage: A non-vascular form of connective tissue composed of CHONDROCYTES embedded in a matrix that includes CHONDROITIN SULFATE and various types of FIBRILLAR COLLAGEN. There are three major types: HYALINE CARTILAGE; FIBROCARTILAGE; and ELASTIC CARTILAGE.Gastric Mucins: Mucins that are found on the surface of the gastric epithelium. They play a role in protecting the epithelial layer from mechanical and chemical damage.Hyaluronic Acid: A natural high-viscosity mucopolysaccharide with alternating beta (1-3) glucuronide and beta (1-4) glucosaminidic bonds. It is found in the UMBILICAL CORD, in VITREOUS BODY and in SYNOVIAL FLUID. A high urinary level is found in PROGERIA.Chondrocytes: Polymorphic cells that form cartilage.Goblet Cells: A glandular epithelial cell or a unicellular gland. Goblet cells secrete MUCUS. They are scattered in the epithelial linings of many organs, especially the SMALL INTESTINE and the RESPIRATORY TRACT.Mast Cells: Granulated cells that are found in almost all tissues, most abundantly in the skin and the gastrointestinal tract. Like the BASOPHILS, mast cells contain large amounts of HISTAMINE and HEPARIN. Unlike basophils, mast cells normally remain in the tissues and do not circulate in the blood. Mast cells, derived from the bone marrow stem cells, are regulated by the STEM CELL FACTOR.Microscopy, Electron: Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.Sublingual Gland: A salivary gland on each side of the mouth below the TONGUE.Chondroitin Sulfates: Derivatives of chondroitin which have a sulfate moiety esterified to the galactosamine moiety of chondroitin. Chondroitin sulfate A, or chondroitin 4-sulfate, and chondroitin sulfate C, or chondroitin 6-sulfate, have the sulfate esterified in the 4- and 6-positions, respectively. Chondroitin sulfate B (beta heparin; DERMATAN SULFATE) is a misnomer and this compound is not a true chondroitin sulfate.Exocrine Glands: Glands of external secretion that release its secretions to the body's cavities, organs, or surface, through a duct.Glycoconjugates: Carbohydrates covalently linked to a nonsugar moiety (lipids or proteins). The major glycoconjugates are glycoproteins, glycopeptides, peptidoglycans, glycolipids, and lipopolysaccharides. (From Biochemical Nomenclature and Related Documents, 2d ed; From Principles of Biochemistry, 2d ed)Collagen Type II: A fibrillar collagen found predominantly in CARTILAGE and vitreous humor. It consists of three identical alpha1(II) chains.Proteoglycans: Glycoproteins which have a very high polysaccharide content.Dog Diseases: Diseases of the domestic dog (Canis familiaris). This term does not include diseases of wild dogs, WOLVES; FOXES; and other Canidae for which the heading CARNIVORA is used.Histological Techniques: Methods of preparing tissue for examination and study of the origin, structure, function, or pathology.Hyaluronoglucosaminidase: An enzyme that catalyzes the random hydrolysis of 1,4-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate. (From Enzyme Nomenclature, 1992) There has been use as ANTINEOPLASTIC AGENTS to limit NEOPLASM METASTASIS.Aggrecans: Large HYALURONAN-containing proteoglycans found in articular cartilage (CARTILAGE, ARTICULAR). They form into aggregates that provide tissues with the capacity to resist high compressive and tensile forces.Immunohistochemistry: Histochemical localization of immunoreactive substances using labeled antibodies as reagents.Connective Tissue: Tissue that supports and binds other tissues. It consists of CONNECTIVE TISSUE CELLS embedded in a large amount of EXTRACELLULAR MATRIX.Cartilage, Articular: A protective layer of firm, flexible cartilage over the articulating ends of bones. It provides a smooth surface for joint movement, protecting the ends of long bones from wear at points of contact.Trypan Blue: A diazo-naphthalene sulfonate that is widely used as a stain.Encyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)Tilia: A plant genus of the family TILIACEAE. Some species in this genus are called Limetree which is nearly the same as the common name for lime (CITRUS AURANTIIFOLIA). Some people are allergic to the POLLEN.2-Propanol: An isomer of 1-PROPANOL. It is a colorless liquid having disinfectant properties. It is used in the manufacture of acetone and its derivatives and as a solvent. Topically, it is used as an antiseptic.Isothiuronium: An undecenyl THIOUREA which may have topical anti-inflammatory activity.Ophiostoma: A genus of fungi in the family Ophiostomataceae, order OPHIOSTOMATALES. Several species are the source of Dutch elm disease, which is spread by the elm bark beetle.Periosteum: Thin outer membrane that surrounds a bone. It contains CONNECTIVE TISSUE, CAPILLARIES, nerves, and a number of cell types.Paneth Cells: Differentiated epithelial cells of the INTESTINAL MUCOSA, found in the basal part of the intestinal crypts of Lieberkuhn. Paneth cells secrete GROWTH FACTORS, digestive enzymes such as LYSOZYME and antimicrobial peptides such as cryptdins (ALPHA-DEFENSINS) into the crypt lumen.Mechanics: The branch of physics which deals with the motions of material bodies, including kinematics, dynamics, and statics. When the laws of mechanics are applied to living structures, as to the locomotor system, it is referred to as BIOMECHANICAL PHENOMENA. (From Dorland, 28th ed)Osteogenesis: The process of bone formation. Histogenesis of bone including ossification.Stem Cells: Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.Fetal Alcohol Spectrum Disorders: An umbrella term used to describe a pattern of disabilities and abnormalities that result from fetal exposure to ETHANOL during pregnancy. It encompasses a phenotypic range that can vary greatly between individuals, but reliably includes one or more of the following: characteristic facial dysmorphism, FETAL GROWTH RETARDATION, central nervous system abnormalities, cognitive and/or behavioral dysfunction, BIRTH DEFECTS. The level of maternal alcohol consumption does not necessarily correlate directly with disease severity.Mesenchymal Stromal Cells: Bone-marrow-derived, non-hematopoietic cells that support HEMATOPOETIC STEM CELLS. They have also been isolated from other organs and tissues such as UMBILICAL CORD BLOOD, umbilical vein subendothelium, and WHARTON JELLY. These cells are considered to be a source of multipotent stem cells because they include subpopulations of mesenchymal stem cells.Developmental Disabilities: Disorders in which there is a delay in development based on that expected for a given age level or stage of development. These impairments or disabilities originate before age 18, may be expected to continue indefinitely, and constitute a substantial impairment. Biological and nonbiological factors are involved in these disorders. (From American Psychiatric Glossary, 6th ed)Morale: The prevailing temper or spirit of an individual or group in relation to the tasks or functions which are expected.Cartilage Diseases: Pathological processes involving the chondral tissue (CARTILAGE).Intervertebral Disc: Any of the 23 plates of fibrocartilage found between the bodies of adjacent VERTEBRAE.Xylenes: A family of isomeric, colorless aromatic hydrocarbon liquids, that contain the general formula C6H4(CH3)2. They are produced by the destructive distillation of coal or by the catalytic reforming of petroleum naphthenic fractions. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)Diazonium Compounds

Changes in joint cartilage aggrecan after knee injury and in osteoarthritis. (1/131)

OBJECTIVE: To determine the concentrations of aggrecan fragments in synovial fluid from patients with knee joint injury, osteoarthritis (OA), or acute pyrophosphate arthritis (PPA; pseudogout), and to test their relative reactivity with the 846 epitope, a putative marker of cartilage aggrecan synthesis. METHODS: Samples of knee joint fluid from 385 patients and 9 healthy-knee volunteers were obtained in a cross-sectional study. Study groups were acute PPA/ pseudogout (n = 60), anterior cruciate ligament (ACL) rupture (n = 159), meniscus lesion (n = 129), and primary knee OA (n = 37). The 846 epitope on aggrecan was assayed by competitive solution-phase radioimmunoassay. Aggrecan fragments were assayed by enzyme-linked immunosorbent assay using a monoclonal antibody (1-F21). Cartilage oligomeric matrix protein (COMP), C-propeptide of type II collagen (CPII), bone sialoprotein, matrix metalloproteinases 1 and 3, and tissue inhibitor of metalloproteinases 1 were previously quantified by immunoassays. RESULTS: Reactivity of the 846 epitope was increased in all study groups compared with the reference group, and was highest in patients with primary OA. The median levels (in microg fetal aggrecan equivalents/ml) of the epitope were 0.28 (range 0.24-0.47) in the reference group, 0.48 (range 0.26-1.32) in PPA/pseudogout, 0.61 (range 0.12-2.87) in ACL rupture, 0.53 (range 0.22-3.02) in meniscus lesion, and 0.68 (range 0.31-4.31) in primary OA. The 846 epitope reactivity per microg aggrecan fragments in the joint fluid was higher in late-stage OA than in early-stage OA. Epitope 846 reactivity correlated positively with several markers of matrix turnover, particularly with COMP (r(s) = 0.421) and CPII (r(s) = 0.307). CONCLUSION: The observed differences in 846 epitope reactivity in synovial fluid, and its concentration in relation to aggrecan and other markers of matrix turnover, were consistent with marked ongoing changes in aggrecan turnover after joint injury and in the development of OA. OA is thus a disease characterized by dynamic changes in tissue macromolecule turnover, which is reflected by measurable changes in aggrecan epitopes in the synovial fluid.  (+info)

Differences in the acid-labile component of Candida albicans mannan from hydrophobic and hydrophilic yeast cells. (2/131)

Cell surface hydrophobicity of the opportunistic fungal pathogen Candida albicans has been linked to the level of cell wall protein glycosylation. Previous work demonstrated that outer chain mannosylation, rather than overall glycosylation, correlated with cell surface hydrophobicity. These studies further suggested that the phosphodiester-linked, acid-labile beta-1,2-mannan was the correlating element. The present work tests this hypothesis and extends the previous results. The composition of bulk mannan from hydrophobic and hydrophilic yeast cells, and the acid-labile mannan from both cell types are compared. Compositional analysis shows that the protein, hexose, and phosphorus content of bulk mannan is similar between the two phenotypes. Electrophoretic separation of acid-released and fluorophore-labeled mannan shows that the acid-labile oligomannosides from hydrophobic cells are longer and potentially in greater abundance than those from hydrophilic cells. These results suggest that regulation of a single step in cell wall protein outer chain mannosylation affects the cell surface ultrastructure and phenotype of C.albicans.  (+info)

Induction of dog sperm capacitation by glycosaminoglycans and glycosaminoglycan amounts of oviductal and uterine fluids in bitches. (3/131)

Ejaculated sperm collected from 12 beagle dogs were incubated in canine capacitation medium (CCM), supplemented with 5 microg/ml chondroitin sulfate A (CS), 5 microg/ml hyaluronic acid (HA), or 5 microg/ml heparin (HP) for 7 hr at 38 degrees C in a 5% CO2 in air atmosphere to investigate the effects of glycosaminoglycans (GAGs) on dog sperm capacitation. The percentages of motile sperm, hyperactivated sperm (%HY), and acrosome-reacted sperm (%AR) in all media were examined after 4 hr and 7 hr of incubation. The oviducts and uteri of 9 anestrous and 18 estrous beagle bitches were removed under halothane inhalation anesthesia to measure the total GAG amounts in oviductal and uterine fluids. The lumens of the ampulla of the oviducts, isthmus of the oviducts, and the uterine horns were each flushed with 1 ml HEPES-EDTA fluid. Total GAG amounts in the flush fluids obtained were measured with a spectrophotometer. Sperm motility (51-59%), %HY (79-86%), and %AR (31-36%) in CCM supplemented with CS, HA, or HP were significantly higher after 7 hr of incubation than when incubated in CCM without GAGs (P<0.01 or 0.05). The mean total GAG amounts in the fluids from the ampulla and isthmus of the oviducts and the uterine horns in the estrous bitches were higher than in the anestrous bitches. These results indicate that GAGs in the oviductal and uterine fluids in estrous bitches are associated with in vivo sperm capacitation.  (+info)

Mapping metastases in sentinel lymph nodes of breast cancer. (4/131)

Localization of metastases within the sentinel lymph nodes (SLNs) of breast cancer has not been studied. Forty SLNs from 36 patients with operable primary breast cancers were identified by means of lymphatic mapping with patent blue dye. The junction between the patent blue-stained lymphatic vessel draining the tumor and the SLN was labeled with alcian blue. Metastases within the serially sectioned SLNs were assigned to the alcian blue-labeled side, to the opposite side of the virtually halved nodes, or both. Eight SLNs were negative for metastasis. Eleven SLNs had metastases only in the blue half. Only 4 cases had larger metastases in the nonblue half. Metastases are more likely to be located in the vicinity of the inflow junction of the identifiable lymphatic draining the tumor and the SLN. This should be considered when SLNs are examined, especially when they are halved for different studies.  (+info)

The pathogenesis of duodenal gastric metaplasia: the role of local goblet cell transformation. (5/131)

BACKGROUND AND AIMS: Gastric metaplasia is frequently seen in biopsies of the duodenal cap, particularly when inflamed or ulcerated. In its initial manifestation small patches of gastric foveolar cells appear near the tip of a villus. These cells contain periodic acid-Schiff (PAS) positive neutral mucins in contrast with the alcian blue (AB) positive acidic mucins within duodenal goblet cells. Previous investigations have suggested that these PAS positive cells originate either in Brunner's gland ducts or at the base of duodenal crypts and migrate in distinct streams to the upper villus. To investigate the origin of gastric metaplasia in superficial patches, we used the PAS/AB stain to distinguish between neutral and acidic mucins and in addition specific antibodies to immunolocalise foveolar cell mucin MUC5AC, the foveolar cell secretory product, gastric trefoil factor (TFF1), the mature goblet cell mucin MUC2, and MUC2 core antigen. RESULTS: Cells in focal patches of gastric metaplasia contained secretory granules of both gastric and goblet cell phenotypes. MUC5AC and TFF1 were present as expected in gastric foveolar cells but in addition, MUC2 core antigen, normally present only in the Golgi of intestinal goblet cells, was expressed in secretory granules. Goblet cells in the vicinity of metaplastic patches also expressed both gastric and intestinal antigens. MUC5AC/MUC2 containing goblet cells were most common near the villus tip but were also seen at the base of crypts. Where crypts and Brunner's gland ducts merged they were always seen on the crypt side of the junction. Goblet cells were the only cells to express gastric antigens in these areas. In advanced metaplastic lesions, dual phenotype goblet cells were less evident and fewer cells expressed intestinal mucin antigens. CONCLUSIONS: We suggest that goblet cells that express both intestinal and gastric antigens may represent local precursors of gastric metaplasia undergoing a transition to foveolar-like cells of mixed phenotype at the site of early metaplastic patches. As metaplasia becomes more widespread, a more pure gastric phenotype emerges. This progression is likely to be controlled by local inflammatory signals.  (+info)

Development of conjunctival goblet cells and their neuroreceptor subtype expression. (6/131)

PURPOSE: To investigate expression of muscarinic, cholinergic, and adrenergic receptors on developing conjunctival goblet cells. METHODS: Eyes were removed from rats 9 to 60 days old, fixed, and used for microscopy. For glycoconjugate expression, sections were stained with Alcian blue/periodic acid-Schiffs reagent (AB/PAS) and with the lectins Ulex europeus agglutinin I (UEA-I) and Helix pomatia agglutinin (HPA). Goblet cell bodies were identified using anti-cytokeratin 7 (CK7). Nerve fibers were localized using anti-protein gene product 9.5. Location of muscarinic and adrenergic receptors was investigated using anti-muscarinic and beta-adrenergic receptors. RESULTS: At days 9 and 13, single apical cells in conjunctival epithelium stained with AB/PAS, UEA-I, and CK7. At days 17 and 60, increasing numbers of goblet cells were identified by AB/PAS, UEA-I, HPA, and CK7. Nerve fibers were localized around stratified squamous cells and at the epithelial base at days 9 and 13, and around goblet cells and at the epithelial base at days 17 and 60. At days 9 and 13, M2- and M3-muscarinic and beta2-adrenergic receptors were found in stratified squamous cells, but M1-muscarinic and beta1-adrenergic receptors were not detected. At days 17 and 60, M2- and M3-muscarinic receptors were found in goblet cells, whereas M1-muscarinic receptors were in stratified squamous cells. Beta1- and beta2-adrenergic receptors were found on both cell types. Beta3-adrenergic receptors were not detected. CONCLUSIONS: In conjunctiva, nerves, M2- and M3-muscarinic, and beta1- and beta2-adrenergic receptors are present on developing goblet cells and could regulate secretion as eyelids open.  (+info)

Dynamic regulation of mucus gel thickness in rat duodenum. (7/131)

We examined the dynamic regulation of mucus gel thickness (MGT) in vivo in rat duodenum in response to luminal acid, cyclooxygenase (COX) inhibition, and exogenous PGE(2). An in vivo microscopic technique was used to measure MGT with fluorescent microspheres in urethan-anesthetized rats. Duodenal mucosa was topically superfused with pH 7.0 or pH 2.2 solutions with or without PGE(2) and indomethacin treatments. Glycoprotein concentration of duodenal loop perfusates was measured with periodic acid/Schiff (PAS) or Alcian blue (AB) staining. MGT and perfusate glycoprotein concentration were stable during a 35-min perfusion with pH 7.0 solution. Acid exposure increased MGT and PAS- and AB-positive perfusate glycoprotein concentrations. Indomethacin pretreatment increased both PAS- and AB-positive perfusate glycoprotein at baseline; subsequent acid superfusion decreased perfusate glycoproteins and gel thickness. PGE(2) (1 mg/kg iv) simultaneously increased MGT and PAS-positive perfusate glycoprotein concentrations followed by a transient increase in AB-positive glycoprotein concentration, suggesting contributions from goblet cells and Brunner's glands. Parallel changes in MGT and perfusate glycoprotein concentration in response to luminal acid and PGE(2) suggest that rapid MGT variations reflect alterations in the balance between mucus secretion and exudation, which in turn are regulated by a COX-related pathway. Luminal acid and PGE(2) augment mucus secretion from goblet cells and Brunner's glands.  (+info)

Genetic control of yeast mannan structure: mapping genes mnn2 and mnn4 in Saccharomyces cerevisiae. (8/131)

Two mutations concerned with mannan biosynthesis in the yeast Saccharomyces cerevisiae have been mapping. The mnn2 mutation, which affects the addition to the polysaccharide backbone of the first side-chain D-mannose unit in alpha1-leads to2 linkage, was located on chromosome II linked to the centromere and the gall locus. The mn4 locus, which regulates the synthesis of mannosylphosphate groups on the mannan side chains, was placed on chromosome XI near trp3 and ural and a locus previously reported to regulate the ability of a S. diastaticus strain to bind alcian blue (Friis and Ottolenghi, 1970). The mnn4 mutant also fails to bind alcain blue, but the gene responsible for alcian blud binding in this strain segregates independently from the dye-binding locus of S. diastaticus, and therefore must be a different gene. A diploid heterozygous for mnn4 fails to bind dye, indicating dominance of this mutant genotype. The alcian blue dye binding locus dbll, reported to Friis and Ottolenghi (1970), is also dominant. Thus, there are at least two independent genes that control the formation of the mannosylphosphate units in the mannan side chains, and both have the property of dominance in the mutant form.  (+info)

0, and good lipid stains generally have a LogP>+7). Methanol is an acceptable substitute for ethanol as a potential vehicle for Alcian blue, but isopropanol is not, because, within a few hours all of suspended Alcian blue precipitates if isopropanol is tried as a vehicle. The sample compound with Merck index number 218 has a melting point of 148 °C. It is a tetravalent basic (cationic) dye with a copper (Cu2+, coordination 4 of 6, orbital configuration d9 with Jahn-Teller distortion) phthalocyanine nucleus (CuPc) with three or four pendent isothiouronium side chains imparting its bulkiness and positive charges. In order to qualify as an alcian blue family member there has to be at least 2 side chains and the mixtures often have 3 chains in average to qualify as 8G. Four tetramethylisothiouronium groups per molecule are shown in the picture. ICI had claimed an average of about three side chains per molecule, but analyses by Prof Scotts lab suggested between three and four. Most of them are at ...
Hi Tom: Alcian Blue will stain the glycosaminoglycans in the extracellular matrix, it wont show much cellular morphology. Still, the traditional method is 1% Alcian Blue in 3% acetic acid for a pH of 2.5. At 2.5 both sulfated and carboxylated GAGs are stained. A red nuclear stain, Scarba red or nuclear fast red, gives a nice contrasty counterstain. You might try 0.05% Toluidine blue in dist water with a pH of about 4.0 for 10-15 minutes, dist water rinse and dehydration in 3 changes of actone followed by clearing in fresh xylene. Nice metachromasia of=20 extracellular matrix and you will see some cellular morphology as well. Geoff Tom Schaer wrote: >Greetings List: > >I would like to stain some intervertebral disc x-sections (rat, decalcified) with alcian blue. Interest in cellular morphology of nucleus pulposus, transition and annulus cells. > >What pH of the stain should I use. > >Many thanks, > >tom >-------------------------------------------------- >Thomas P. Sch r, VMD >Asst Prof School ...
Thanks to all who responded to the Alcian blue question. It is definitely a developmental problem with these bones/cartilage. The cells are not producing the required muccopolysaccharides necessary for proper staining. anita ...
Demonstrate the presence of both neutral and acidic mucosubstances with the Thermo Scientific Richard-Allan Scientific Alcian Blue/PAS Special Stain Kit
And APPLE -GREEN BIREFRINGERENCE on POLARISED MICROSCOPY(DIAGNOSTIC). 9) ALCIAN BLUE Stains;-. #Mucin and mucosubstances. 10) ACID FAST Stains;-. #Mycobacterium. 11) FONTANA MASSON:-. #Melanin and Argentaffin cells.. 12) MASSONs TRICHROME Stains:-. #Collagen and connective tissue. 13) VERHOFF-VON-GIESON (VVG):-. #Elastic fibres ( other stain-ORCEIN stain). 14) TOLUIDINE BLUE:-. #Mast cells. 15) LUXOL FAST BLUE:-. #Myelin. 16) BIELSCHOWSKY Stains:-. (uses silver stain) ...
ABSTRACT: Microphytobenthos (MPB) are an important component of shallow-water habitats where there is sufficient light to support benthic photosynthesis. Production of extracellular polymeric substances (EPS) is characteristic of the MPB, and acid polysaccharides (APS) are a major component of these EPS. A simple technique was developed to extract and measure APS concentrations in sediments. Water-extractable APS (wAPS) were extracted with ultra high purity water from lyophilized sediments. After centrifuging to remove the sediment, wAPS in the supernatant was precipitated with alcian blue, a cationic dye. The APS-alcian blue precipitate was removed by filtration. The remaining alcian blue in solution was determined spectrophotometrically and was inversely proportional to the concentration of wAPS in the sediment. Chlorophyll (chl) a, carbohydrate fractions, and wAPS were measured in sediments from 5 contrasting sites in a microtidal salt marsh in the subtropics. wAPS concentrations, expressed ...
Posterior salivary glands. As the gland described above, posterior salivary glands are simple branched tubular glands. They are covered by a connective capsule with muscular fibers surrounding the glandular tubules like thin connections. Each tubule presents two zones formed by different cells. The first or proximal zone (A) consists of globular mucous cells with flattened basal nuclei (Figure 5b). The supranuclear cytoplasm contains high level of PAS (+) and alcian blue (+) pH = 3.5, vesicles denoting the presence of weakly sulphated glycosaminoglycans content. Cylindrical cells with a basal spherical nucleus surrounded by a basophilic cytoplasm are also seen. The second or distal zone (B) present cylindrical cells characterized by its weakly eosinophilic cytoplasm. The nucleus with basal or apical location is observed (Figure 5b). The PAS (+) and Alcian Blue (-) pH = 3.5 reaction in the cytoplasm, suggests the presence of neutral carbohydrates.. Animals kept in the aquarium without food for ...
PAVON, L. F.... Ultrastructural characterization of CD133(+) stem cells bound to superparamagnetic nanoparticles: possible biotechnological applications. JOURNAL OF MICROSCOPY 231 n.3 p. 374-383 SEP 2008. Journal article.
Scores of people around the country are protesting against Drax Plc on the day of its AGM today, with three simultaneous demonstrations in London, Liverpool and York. Campaigners are highlighting Draxs involvement in dirty energy, its contributions to… Read More. ...
INTRODUCTION: The clinical efficacy of infliximab (IFX) for induction of remission in both adults and children with active Crohns disease (CD) has been well documented. Recently, so-called "deep remission" defined as mucosal healing has become the ultimate endpoint of the most recent therapeutic advances for CD. However, endoscopic evidence of mucosal healing is not necessarily associated with histological evidence of suppression of inflammation. AIM: Since data on that issue are limited, especially in the paediatric population, the aim of this study was to assess the impact of induction therapy with IFX on deep microscopic remission in paediatric patients with CD ...
D: The skeleton of a wild-type mouse (right) and Wwtr1tm1Whun/Wwtr1tm1Whun mouse (left) embryos stained with Alizarin red and Alcian blue to stain bone (red) and cartilage (blue), respectively. Note the slightly shorter skeleton of the homozygous mouse embryo. G & H: Hematoxylin and eosin staining of longitudinal kidney sections from 8 week old wild-type (G) and homozygous mutant mouse (H), showing numerous cysts in the corticomedullary region of the homozygous mouse kidney. Magnification: x20 ...
FIG. 3. Whole mount in situ hybridization pattern of Xbap [cc: now called nkx3-1] (A, B, C, D, E, and F) and gsc (G, H, I, J, and K) and Alcian blue staining to visualize cartilage (L). All embryos were photographed at 701 magnification except for (D) at 601. (A) Lateral view of a stage 36 embryo showing both regions of facial staining and the region of gut expression (indicated by g in the figure). (B) Anterior view of the embryo pictured in (A). The paired anterior regions of expression lie on either side of the future mouth opening (M). (C) Dorsal view of a stage 37 embryo showing the forked appearance of the Xbap staining. (D) Lateral view of the left side of a stage 42 embryo showing staining of the anterior gut tube and facial staining. (E) Dorsal view of a stage 40 embryo. Xbap staining now marks crescent-shaped regions on either side of the future mouth opening (M). (F) Dorsal view of the embryo shown in (D). The region of staining corresponding to the palatoquadrate cartilage (PQ) is ...
Hyaluronate electophoresis - posted in Biochemistry: Hi everyone! I would like to ask if anyone has any experience in electrophoresis and detection of hyaluronate. I did PAGE of a digest mixture (consisting of HA chains of various lengths) according to numerous protocols (15% and 10% gel in TBE, linear gradient 20%-3% and 18%-1,5%, with samples in NaOAc, NH4HCO3, NaOAc+HCl, dH20 or TBE, with the voltage of 300V and 100V and detection by Alcian Blue in water, water+2% acetic acid or wat...
Free flashcards to help memorize facts about UND 363 Alcian Blue 1.0. Other activities to help include hangman, crossword, word scramble, games, matching, quizes, and tests.
The colour database is collected and created by the experts of Chemnet. There are more than 1,000 colours in this database about the colours physicochemical property, applications, synthetic methods and so on. It will be updated in time.
Looking for online definition of Alcian yellow-toluidine blue Leung stain in the Medical Dictionary? Alcian yellow-toluidine blue Leung stain explanation free. What is Alcian yellow-toluidine blue Leung stain? Meaning of Alcian yellow-toluidine blue Leung stain medical term. What does Alcian yellow-toluidine blue Leung stain mean?
Hito Alcian Blue pH-2.5 OptimStain™ Kit is formulated for the staining of acid mucins, sulfated / carboxylated acid mucopolysaccharides and sulfated / carboxylated sialomucins (glycoproteins).
Orcein-alcian blue staining, a new method for the simultaneous demonstration of sulphated and sialomucins in gastrointestinal epithelium was compared with the standard high iron diamine-alcian blue technique. Sections were oxidised with potassium permanganate and decolourised in oxalic acid. They were stained with orcein for four hours, differentiated for a few seconds in acid alcohol, and then counterstained with alcian blue for half to one minute. There was a good correlation of results between the two methods. Orcein-alcian blue is a safer, cheaper, and quicker method than high iron diamine-alcian blue which can be safely introduced into routine laboratories for the study of acid mucins in the gastrointestinal diseases.. ...
Looking for online definition of Blue Cell Tumor in the Medical Dictionary? Blue Cell Tumor explanation free. What is Blue Cell Tumor? Meaning of Blue Cell Tumor medical term. What does Blue Cell Tumor mean?
Images: Cuboidal lining , Glycogen , Aggressive serous cyst , Cysts in serous cystadenomas are composed of a single layer of clear cuboidal cells. These cells usually form flat sheets that line the cysts and only rarely form papillae that project into the cystic spaces. The neoplastic cells have clear cytoplasm because they contain abundant intracytoplasmic glycogen. They do not produce mucin. The nuclei are small, round, and have uniform chromatin and inconspicuous nucleoli. Atypia and mitoses are essentially absent. The central stellate scar and the stroma separating the cysts are composed mostly of relatively acellular collagenous connective tissues, but the stroma may contain entrapped islets of Langerhans and acini. Special stains will highlight the abundant glycogen and absence of mucin. A periodic acid Schiff (PAS) stain will be strongly positive and sensitive to diastase digestion. Stains for mucin, including a mucicarmine and alcian blue stains, will be negative, as will stains for ...
Periodic acid-Schiff (PAS) is a staining method used to detect polysaccharides such as glycogen, and mucosubstances such as glycoproteins, glycolipids and mucins in tissues. The reaction of periodic acid oxidizes the vicinal diols in these sugars, usually breaking up the bond between two adjacent carbons not involved in the glycosidic linkage or ring closure in the ring of the monosaccharide units that are parts of the long polysaccharides, and creating a pair of aldehydes at the two free tips of each broken monosaccharide ring. The oxidation condition has to be sufficiently regulated so as to not oxidize the aldehydes further. These aldehydes then react with the Schiff reagent to give a purple-magenta color. A suitable basic stain is often used as a counterstain. PAS diastase stain (PAS-D) is PAS stain used in combination with diastase, an enzyme that breaks down glycogen. Alcian blue/periodic acid-Schiff (AB/PAS or AB-PAS) uses alcian blue before the PAS step. PAS staining is mainly used for ...
TY - JOUR. T1 - A Review on Adsorption of Cationic Dyes using Activated Carbon. AU - Corda, Nikita Chrishel. AU - Kini, M. Srinivas. PY - 2018/1/9. Y1 - 2018/1/9. N2 - In this article efficiency of activated carbon as a potent adsorbent of cationic dyes has been reviewed. Non-biodegradable nature of pollutants and their removal in the present generation is a great challenge. Therefore, extensive study on adsorption of these classes of pollutants from water bodies is being carried out. Methylene blue (majorly) a dye seen in the effluent streams of textile, printing, paper industries along with some of the commonly used cationic dyes in process industries and their sorption on activated carbon are reviewed here. High cost of commercially activated carbon which is a limitation to its extensive use have paved way for study of adsorption by naturally obtained and extracted activated carbon from agricultural wastes and various other sources. The purpose of this review paper is to summarize the ...
We have developed a reliable and sensitive immunohistochemical staining technique which allows the simultaneous demonstration of two different antigens expressed in or on the same cell (referred to as mixed labeling), together with the evaluation of the general histopathological appearance of the tissue. The staining procedure combines a three-step (streptavidin-biotin) immunogold-silver staining (IGSS) with a three-step immunoenzymatic labeling. For this purpose, we investigated the compatibility ofIGSS with various substrates of peroxidase or alkaline phosphatase (AP). Highly reliable and discernible mixed labeling was achieved only after iniriallabeling with IGSS followed by AP labeling using the substrates naphthol AS-MX phosphate/Fast Blue or naphthol AS-HI phosphate/New Fuchsin, respectively. To ensure utmost specificity, we applied FlTC-conjugated mouse monoclonal antibodies and rabbit anti-FlTC immunoglobulins visualized by AP-labeled immunoglobulins and the respective substrate in a ...
We have developed a reliable and sensitive immunohistochemical staining technique which allows the simultaneous demonstration of two different antigens expressed in or on the same cell (referred to as mixed labeling), together with the evaluation of the general histopathological appearance of the tissue. The staining procedure combines a three-step (streptavidin-biotin) immunogold-silver staining (IGSS) with a three-step immunoenzymatic labeling. For this purpose, we investigated the compatibility ofIGSS with various substrates of peroxidase or alkaline phosphatase (AP). Highly reliable and discernible mixed labeling was achieved only after iniriallabeling with IGSS followed by AP labeling using the substrates naphthol AS-MX phosphate/Fast Blue or naphthol AS-HI phosphate/New Fuchsin, respectively. To ensure utmost specificity, we applied FlTC-conjugated mouse monoclonal antibodies and rabbit anti-FlTC immunoglobulins visualized by AP-labeled immunoglobulins and the respective substrate in a ...
Demonstrates neutral and acidic mucosubstances on tissue at pH 2.5 imparts a blue color to the acidic mucins and other carboxylated or weakly sulphated acid mucosubstances. Periodic Acid Schiff (PAS) reaction is then used to stain basement membranes, glycogen and neutral mucosubstances pink to red. Mixtures of neutral and acidic mucosubstances will appear purple due to positive reactions with both Alcian Blue and PAS.. Learn More ...
Demonstrates neutral and acidic mucosubstances on tissue at pH 2.5 imparts a blue color to the acidic mucins and other carboxylated or weakly sulphated acid mucosubstances. Periodic Acid Schiff (PAS) reaction is then used to stain basement membranes, glycogen and neutral mucosubstances pink to red. Mixtures of neutral and acidic mucosubstances will appear purple due to positive reactions with both Alcian Blue and PAS.. Learn More ...
Posterior gut (Alcian Blue-PAS) from fish fed (A) Fish oil-based diet (FO), (B) FO- and MOS-based diet (FOMOS), (C) soy bean meal-based diet (SBO), and (D) SBO-
Checkpoint inhibitors, including nivolumab and pembrolizumab, are novel therapies that have demonstrated significant efficacy in the management of metastatic malignancy.10 The immunological adverse event profile related to these therapies is broad, with rates of enterocolitis as high as 30%.7,11 The median time of onset of symptoms is 6-7 weeks from initiation of therapy but can vary, with some reports as late as 6 months, as in our case.11 The colitis is manifested by diarrhea, defined as greater than 4 stools per day, with blood or mucous, abdominal discomfort or cramping, and radiographic or endoscopic evidence of inflammation.12 The pathophysiology is characterized by lymphocytic infiltration in the lamina propria and epithelium of the involved mucosa. This inflammation can be transmural, and spontaneous bowel perforations have been reported.13 Interestingly, the remainder of the GI tract is rarely involved. Management includes exclusion of other infectious etiologies followed by initiation ...
Histology: the tumour has an expansive growing pattern mainly surrounded by fibrous tissue and it is composed by solid and glandular/pseudoglandular components. The cells are small, myoepithelial and epithelial cells, polarized around two types of spaces - glandular or pseudolumina. The true glandular spaces are surrounded by luminal cells and contain neutral mucin (PAS positive); the pseudolumina are stromal spaces filled by cylinders of hyaline material, surrounded by basal/myoepithelial cells; these spaces stain for Alcian Blue and collagen IV. The cells in the solid areas and around the cylinders stain for p63, smooth-muscle actin as well as for high-molecular-weight keratins. Cells around the lumina stain for low-molecular-weight keratin (for example keratin 7), as well as for CD117. On the cystic component, squamous metaplasia can be observed. Focally mainly in the solid areas the cells are bigger with evident nucleoli and the mitotic index is higher. Necrotic foci can be observed ...
The Alcian Blue (pH 2.5) Stain Kit is intended for use in the histological visualization of sulfated and carboxylated acid mucopolysaccharides and sulfated and carboxylated sialomucins (glycoproteins ...
산약 (Dioscoreae Rhizoma)의 수용성 점액물질(mucilage)은 Polysaccharide와 Protein으로 구성되어 있는 proteoglycan으로 알려져 왔다. 본 연구에서는 종래의 mucilage 정제법인 에탄올 침전법과는 다른 새로운 방법으로 mucilage 정제를 실시하고자 하였으며, 산약의 mucilage에 대한 정량법을 확립하고, 산약의 가공에 따른 mucilage 함량변화를 알아보았다. 시료산약은 재배품종으로서 시판되고 있는 두 가지 형태의 마 (곤봉형 마, 杖형 마)를 사용하였고, 각각의 생품과 증건품을 가지고 실험하였다. 산약의 물 추출액을 투석한 추, Bio-gel P-100과 Sephadex G-150을 사용하여 겔 여과한 결과, polysaccharide 부분과 Protein 부분이 서로 분리되어 나타나는 것으로 보아, 산약의 mucilage 에는 Protein이 함유되어 있지 않음을 확인할 수 있었다. 한편, mucilage 정량 방법으로는 산약의 mucilage성분이 alcian blue 와 ...
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This FlowCellectMitoDamage Kit for Flow Cytometry includes MitoSense Red, a fluorescent cationic dye that accumulates in the mitochondria & is responsive to mitochondrial potential changes. Find MSDS or SDS, a COA, data sheets and more information.
Just to clarify for anyone that is unfamiliar, phagocytosis is the process in which one cell engulfs another. In this case, the big, blue cell is about to swallow the little, purple cell ... And the purple cell is thinking: "Oh, no ...
Various miRNAs have been reported to regulate the chondrogenic differentiation of bone marrow mesenchymal stem cells (BMSCs); however, whether miR-134 plays a role in this biological process remains undetermined. In the present study, we first evaluated the chondrogenic differentiation of BMSCs by Alcian blue staining, and examined the miR-134 expression by quantitative real-time PCR (qRT-PCR) during this process. And miR-134 inhibitor was used to investigate the functions of miR-134 in chondrogenic differentiation of BMSCs by Alcian blue staining, qRT-PCR, and Western blot. Subsequently, the correlation between miR-134 and SMAD6 was assessed via bioinformatics analysis and dual-luciferase reporter assay. Finally, the role of SMAD6 in chondrogenic differentiation of BMSCs was also determined through Alcian blue staining, qRT-PCR, and Western blot. As results showed that miR-134 expression was significantly down-regulated during chondrogenic differentiation, and inhibition of miR-134 obviously ...
Multiple specimens taken at oesophageal suction biopsy were obtained from 56 patients, of whom 44 had symptoms of gastro-oesophageal reflux and 24 had endoscopic evidence of erosive oesophagitis. Biopsies were examined independently by two histopathologists for the following criteria for reflux: epithelial hyperplasia, vascular dilatation and congestion, neutrophil infiltration, and eosinophil infiltration. The incidence of these criteria in patients with and without endoscopic evidence of oesophagitis or symptoms of reflux was investigated. It was concluded that vascular dilatation and epithelial hyperplasia, defined as basal zone thickness greater than or equal to 15% and papillary elongation greater than or equal to 66%, can be detected most reliably, but their diagnostic accuracy is limited unless multiple biopsies are examined.. ...
The cationic dye, chrysoidine, has been used for the first time as a probe for the indirect photometric detection of cations. The dye has been used as a probe at concentrations of 5 mM, which is roughly an order of magnitude higher than for other cationic dyes used previously for the same purpose, in order to minimise electromigrational dispersion. Baseline instability was minimised by a combination of coating the capillary with poly(ethyleneimine), addition of a neutral polymer to the electrolyte, and the application of a small amount (20 mbar) of hydrodynamic pressure during the separation. Separation of a mixture containing alkali metals, alkaline earths, transition metals and lanthanides was achieved by the addition of 2-hydroxyisobutyric and lactic acid as complexing agents. Excellent peak shapes were observed over a wide range of analyte mobilities due to the moderate mobility of the probe. The high absorptivity (26 733 l mol−1 cm−1) provided by chrysoidine in comparison with typically ...
Pig duodenum stained to differentiate carboxylated mucus in Brunners glands (yellow) from sulfonated mucus in gobletcells (blue-green). Alcian blue pH 1.0 + periodic acid + sodium metabisulfite + Alcian yellow = Brazilliant ...
Patients should have had a liver biopsy showing at least Stage 3 disease prior to being considered for this study. A baseline liver biopsy is necessary for inclusion in the study. Baseline liver biopsies can be performed within six months of entering the study.. In patients with cirrhosis and endoscopic evidence of portal hypertension, a biopsy within the last 2 years is acceptable as the baseline biopsy. For patients with established cirrhosis on liver biopsy and no portal hypertension, a biopsy within 12 months can be used as the baseline biopsy if it is available for evaluation by the Pathology core. All these patients will still require liver biopsy at 2 years and 4 years. The decision to biopsy at 2 and 4 years is also a clinical decision and in the presence of clinical progression or coagulopathy, or where there may be a risk from liver biopsy, the Investigator should call the PI, Dr. Afdhal for a waiver of biopsy. Patients with Ishak Stage 3 and 4 require a biopsy within 6 months of ...
In the gastric mucosa of a patient with Helicobacter pylori infection and a precancerous lesion called "incomplete intestinal metaplasia" (Genta stain), the gastric cells lining a gland have been replaced by absorptive intestinal cells with visible brush border and goblet cells, which are normally present in the intestine, but not in the stomach. Goblet cells contain acidic mucins stained blue by Alcian blue. This type of precancerous transformation is caused by long-term infection with H. pylori, visible in the lumen of the gland as curved bacterial rods stained black by silver stain. A few bacteria invade the goblet cells and can be found within blood capillaries, where they bind to red blood cells (see Aspholm et al. 2006).. ...
The fixation of dissociated chick embryonic cells in the presence of ruthenium red or alcian blue revealed a remarkable reduction in the binding of these cations to cell surfaces following tryptic digestion at 37 °C, but not after trypsin used at 4 °C or EDTA.. The reconstitution of anionic sites present over the cell plasma membrane was studied in retina, liver and heart cells from embryos of different ages, cultivated according to a method which promotes cell reaggregation. All these cells regenerated ruthenium red-stainability within the first hour of culture. Culturing at 15 °C, substitution of saline for the culture medium or addition of puromycin or of neuraminidase to the culture media did not affect this restitutive process. Neuraminidase and hyaluronidase digestion were not able to alter the ruthenium red-binding, once it was present.. From these data and from the reports available in the literature it has been concluded that the anionic binding-sites cannot be identified with the ...
* found in: Methyl Orange, Trypan Blue Solution 0.4%, Xylene Cyanol FF, Neutral Red, Safranin O, Alcian Blue 8 GX, Nitroblue Tetrazolium Chloride, Congo..
A A beta 42 abl acid phosphatase ACSM1 actin-general actin-alpha cardiac actin-alpha smooth muscle actin-muscle specific AE1/AE3 (cytokeratin) AF4 AGR2 AKT1 albumin alcian blue ALK alkaline phosphatase ALL1 alpha-1-antichymotrypsin alpha-1-antitrypsin alpha-fetoprotein alpha-lactalbumin AMACR AML1 androgen receptor APC API2-MALT1 Apolipoprotein D arginase1 ATM ...
Fingerprint Dive into the research topics of Ultrastructural characterization of maize (Zea mays L.) kernels exposed to high temperature during endosperm cell division. Together they form a unique fingerprint. ...
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... is the most versatile and widely used technique for carbohydrate visualization. From a diagnostic point of view...
A study by light microscopy, using Leishman's stain alone or Leishman's stain followed by nigrosin, showed the presence of capsules on gonococci of two strains subcultured from subcutaneous chambers in guinea pigs. With the Alcian blue method of preparation for electron microscopy, gonococci of both strains recently grown in vivo showed densely stained capsules on some cells, while others in the same preparation showed only irregular masses of dense material on their surfaces with strands connecting adjacent bacteria. Treatment with antiserum, complement and conglutinin revealed irregular masses and strands of extracellular material with fixatives that did not contain Alcian blue.
The relationship between Ca++ and pinocytosis was investigated in Amoeba proteus. Pinocytosis was induced with 0.01% alcian blue, a large molecular weight dye which binds irreversibly to the cell surface. The time-course and intensity of pinocytosis was monitored by following the uptake of [3H]SUCROSE. When the cells are exposed to 0.01% alcian blue, there is an immediate uptake of sucrose. The cells take up integral of 10% of their initial volume during the time-course of pinocytosis. The duration of pinocytosis in the amoeba is integral of 50 min, with maximum sucrose uptake occurring 15 min after the induction of pinocytosis. The pinocytotic uptake of sucrose is reversibly blocked at 3 degrees C and a decrease in pH increases the uptake of sucrose by pinocytosis. The process of pinocytosis is also dependent upon the concentration of the inducer in the external medium. The association between Ca++ and pinocytosis in A. proteus was investigated initially by determining the effect of the ...
Recent studies described the phenotype of Fezl-deficient mice. Similarly to zebrafish, mammalian Fezl is expressed in the telencephalon and diencephalon (Hirata et al., 2004; Mutsuga et al., 2005). In the mouse telencephalon, Fezl is required for fate specification and axonal projections of cortico-spinal motor neurons, subplate cortical neurons and deep-layer pyramidal neurons (Chen et al., 2005a; Chen et al., 2005b; Hirata et al., 2004; Molyneaux et al., 2005). A close homolog of Fezl, denoted Fez, is expressed in the mouse hypothalamus in partially overlapping domains with Fezl and fez-fezl double deficient mouse displays defects in diencephalic subdivisions (Hirata et al., 2006a; Hirata et al., 2006b). A similar role for zebrafish Fezl in diencephalic patterning was also reported (Jeong et al., 2007).. The precise regulation of Otp, DA and IT by the tof m808 hypomorph (Figs 1 and 3) allows us to separate the role of Fezl in regional patterning from its more selective role in cell ...
It is widely believed that the increased cancer risk in UC is primarily acquired secondary to colonic inflammation.9,10 Although it is inherently logical that severely inflamed mucosa will confer an increased risk of neoplastic transformation, until recently there were few data to support this perception. However, a recent study from our centre has demonstrated a highly significant association between the severity of colorectal inflammation and neoplasia risk.5. There is a wide spectrum of colonoscopic appearances in patients with UC, all of which provide the colonoscopist with much information about that patients disease history. Patients may have endoscopic evidence of active inflammation (for example, friable mucosa or ulceration) although many patients will have no macroscopically active disease. Irrespective of acute changes, patients may have chronic endoscopic changes, such as scarring and post-inflammatory polyps, which signify previous significant inflammation but are unrelated to ...
Alcian blue stain. Tortora, Gerard J.; Derrickson, Bryan. Introduction to the Human Body. The Essentials of Anatomy and ...
Alcian blue stain. ...
Early lesions, rich in acid mucopolysaccharides, stain positively with alcian blue. When medullary thyroid cancer is present, ... Luxol fast blue staining identifies myelin sheathing of some fibers, and lesional cells react immunohistochemically for S-100 ...
Alcian blue will give a blue staining to the stromal matrix material. Mucicarmine will also highlight the stromal matrix (pink ...
Micrograph of Barrett's esophagus (left of image) and normal stratified squamous epithelium (right of image), Alcian blue stain ... High-magnification micrograph of Barrett's esophagus showing the characteristic goblet cells, Alcian blue stain ... Use of the histochemical stain Alcian blue pH 2.5 is also frequently used to distinguish true intestinal-type mucins from their ...
... alcian blue, Verhoeff hematoxylin, crocein scarlet combined with acidic fuchsine and saffron. At pH 2.5, alcian blue is fixed ... Movat's stain showing thickening of the spongiosa layer (blue) in myxomatous degeneration of the aortic valve. Movat's stain ...
Alcian blue/periodic acid-Schiff (AB/PAS or AB-PAS) uses alcian blue before the PAS step. PAS staining is mainly used for ... dyes Methyl blue Methyl violet Methylene blue New methylene blue Potassium ferricyanide Potassium ferrocyanide Prussian blue ... Carboxyfluorescein DyLight Fluor, a product line of fluorescent dyes Egyptian blue Eosin Erythrosine Fluorescein Fluorescein ...
Whipple's disease: Alcian blue with apparently eosin counterstain enlarged villus with many macrophages. ...
Micromass cultures of C3H-10T1/2 cells at varied oxygen tensions stained with Alcian blue. ...
Use of the histochemical stain Alcian blue pH 2.5 is also frequently used to distinguish true intestinal-type mucins from their ...
Alcian blue-PAS stain Elsevier Article Locator Modern Pathology - Primary Mucoepidermoid Carcinoma and Sclerosing ...
Other dyes used in connection with van Gieson staining include: Alcian blue Amido black 10B Verhoeff's stain Jocelyn H. Bruce- ...
... alcian blue MeSH D03.438.473.133 --- captan MeSH D03.438.473.155 --- carbolines MeSH D03.438.473.193 --- chlorisondamine MeSH ... methylene blue MeSH D03.494.741.533 --- moricizine MeSH D03.494.741.550 --- nonachlazine MeSH D03.494.741.575 --- perazine MeSH ...
When vessels are stained with cationic dyes such as Alcian blue stain, transmission electron microscopy shows a small, ...
Adorable Air Formation Airiel Alcian Blue Alison's Halo Apollo Heights Asobi Seksu Astreal The Away Days The Boo Radleys The ...
Alcian Blue "Alcian Blue" ES1014 - Ashley Naylor "Ashley Naylor's Four Track Mind" ES1013 - The Situation "The Situation" ...
... shades of blue Alcian blue stain Leung, Jennifer K.; Gibbon, Kevin J.; Vartanian, Robert K. (1996-06-01). "Rapid Staining ... Alcian yellow is used in histology in conjunction with toluidine blue to help visualize H. pylori. The resulting structures are ... Alcian yellow is an azo dye with the formula (CH3[(CH3N)2CS]2C6H2SNCC6H4)2N2. Its structure consists of a largely conjugated ... stained as follows: - Mucin: yellow - H. pylori: blue - Other cellular components/backgroun: ...
Alcian blue or alcian blue (/ˈælʃən/) is any member of a family of polyvalent basic dyes, of which the Alcian blue 8G (also ... Solid Alcian blue is a combustible powder and should never be handled close to heat or a naked flame. Heating Alcian blue ... In addition to its use as a dye or stain Alcian blue also finds other material science uses. Alcian blue has been used as an ... Scott also states that Alcian green was merely a mixture of Alcian blue and Alcian yellow and not a single compound, which is ...
Alcian Blue was a shoegaze and new wave-influenced band operating out of the Washington, DC suburb of Takoma Park from 1998 to ... "Alcian Blue - Alcian Blue - Songs, Reviews, Credits - AllMusic". AllMusic. Retrieved 2016-08-09. http://www.safraninsound.com/ ... Slow Colorless Stare CD (2001, Safranin Sound) Alcian Blue CD (2006, Elephant Stone) Angelica Take Me Down EP (2003, Safranin ...
Alcian blue stain. *Movat's stain. *Jaswant Singh-Bhattacharji (JSB) stain. Tissue stainability. *Acidophilic ...
Nile blue[edit]. Nile blue (or Nile blue A) stains nuclei blue. It may be used with living cells. ... Coomassie blue[edit]. Coomassie blue (also brilliant blue) nonspecifically stains proteins a strong blue colour. It is often ... Methylene blue[edit]. Methylene blue is used to stain animal cells, such as human cheek cells, to make their nuclei more ... Nile red (also known as Nile blue oxazone) is formed by boiling Nile blue with sulfuric acid. This produces a mix of Nile red ...
Alcian Blue was a shoegaze and new wave-influenced band operating out of the Washington, DC suburb of Takoma Park from 1998 to ... "Alcian Blue - Alcian Blue - Songs, Reviews, Credits - AllMusic". AllMusic. Retrieved 2016-08-09. http://www.safraninsound.com/ ... Slow Colorless Stare CD (2001, Safranin Sound) Alcian Blue CD (2006, Elephant Stone) Angelica Take Me Down EP (2003, Safranin ...
Alcian blue or alcian blue (/ˈælʃən/) is any member of a family of polyvalent basic dyes, of which the Alcian blue 8G (also ... Solid Alcian blue is a combustible powder and should never be handled close to heat or a naked flame. Heating Alcian blue ... In addition to its use as a dye or stain Alcian blue also finds other material science uses. Alcian blue has been used as an ... Scott also states that Alcian green was merely a mixture of Alcian blue and Alcian yellow and not a single compound, which is ...
... alcian blue include Using Unfixed, Frozen Tissues to Study Natural Mucin Distribution, Adult Zebrafish Injury Models to ... Alcian Blue: A copper-containing dye used as a gelling agent for lubricants, for staining of bacteria and for the dyeing of ...
After injection of Alcian blue at LSP (lumbar sacral point) of the GV meridian, we observed a blue line at the GV meridian ... Acupuncture meridian Alcian blue Bonghan duct system Lumbar sacral point This is a preview of subscription content, log in to ... 2007) Alcian Blue Staining Technique for Tracing the Governing Vessel (GV) Meridian in Rat. In: Magjarevic R., Nagel J.H. (eds ... Only very recently we found a staining dye, Alcian blue that stains the acupuncture meridian and were able to trace the ...
Alcian Blue-tetrakis(methylpyridinium) chloride; CAS Number: 123439-83-8; Synonym: A,B,C,D-tetrakis(Pyridiniomethyl) Cu(II) ... Alcian Blue-. tetrakis(methylpyridinium) chloride Synonym: A,B,C,D-. tetrakis(Pyridiniomethyl) Cu(II) phthalocyanine chloride ... This dye is similar in structure and properties to Alcian Blue 8GX, with pyridiniomethyl substituents in each ring instead of ...
Alcian Blue in conjunction with PAS (Mowry, 1956) is commonly used to differentiate acid and neutral mucopolysaccharides. It ...
Stain strongly sulfated mucins with Alcian Blue Stain Kit ab150661. Abcam offers , 1,000 assay kits cited in , 3,500 ... ab150661 (Alcian Blue pH 1.0) staining strongly sulphated mucins (blue) in formalin fixed paraffin embedded human colon, ... incubate in Alcian Blue pH 1.0 for 30 min. - rinse in water. - stain in nuclear fast red for 5 min. - rinse in water. - ... Alcian Blue staining protocol summary:. - deparaffinize sections if necessary and hydrate. - incubate in acetic acid for 3 min ...
Ingrain Blue 1. SMILES. CN(C)C(=[N+](C)C)SCC1=CC2=C(C=C1)C3=NC4=C5C=C(C=CC5=C([N-]4)N=C6C7=C(C=CC(=C7)CSC(=[N+](C)C)N(C)C)C(=N6 ...
... From:. Anita Jennings ,[email protected],. Thanks to all who responded to the Alcian blue ...
... From:. Geoff McAuliffe Hi Tom: Alcian Blue will stain the glycosaminoglycans in the ... Still, the traditional method is 1% Alcian Blue in 3% acetic acid for a pH of 2.5. At 2.5 both sulfated and carboxylated GAGs ... with alcian blue. Interest in cellular morphology of nucleus pulposus, transition and annulus cells. > >What pH of the stain ... You might try 0.05% Toluidine blue in dist water with a pH of about 4.0 for 10-15 minutes, dist water rinse and dehydration in ...
Demonstrate the presence of both neutral and acidic mucosubstances with the Thermo Scientific Richard-Allan Scientific Alcian ... Includes: Alcian Blue pH 2.5 stain solution, periodic acid solution (0.5%) and Schiff reagent ... Can be used alone to stain acid mucins and other carboxylated and sulfated acid mucosubstances blue on tissue sections ...
Leica Alcian Blue Special Stain Kit Consumables Leica Alcian Blue Special Stain Kit *Product ... Alcian Blue is normally prepared at an acidic pH of 2.5 and is used to identify acid mucopolysaccharides and acetic mucins. ...
Orcein-alcian blue is a safer, cheaper, and quicker method than high iron diamine-alcian blue which can be safely introduced ... Orcein-alcian blue staining, a new method for the simultaneous demonstration of sulphated and sialomucins in gastrointestinal ... Orcein-alcian blue staining: a new technique for demonstrating acid mucins in gastrointestinal epithelium. ... Orcein-alcian blue staining: a new technique for demonstrating acid mucins in gastrointestinal epithelium. ...
Our lab has used the Alcian blue - Alizarin red staining method with certain modifications to characterize skeleton deformities ... staining the cartilage is done with 0.02% alcian blue (as in your protocol) or 0.2% alcian blue (as in MBC paper)?. Thanks ... Alcian blue 8GX (prepared in ethanol/ glacial acetic acid, 7:3) for 1 to 2 days.. Note: Cartilage tissues will be stained blue ... Zhang, P. (2012). Alcian Blue - Alizarin Red Staining of Mouse Skeleton. Bio-protocol 2(8): e162. DOI: 10.21769/BioProtoc.162. ...
Looking for abbreviations of ABRBC? It is Alcian Blue on Red Blood Cell Membrane. Alcian Blue on Red Blood Cell Membrane listed ... Alcian Blue on Red Blood Cell Membrane - How is Alcian Blue on Red Blood Cell Membrane abbreviated? https://acronyms. ... a href=https://acronyms.thefreedictionary.com/Alcian+Blue+on+Red+Blood+Cell+Membrane,ABRBC,/a,. *Facebook ... redirected from Alcian Blue on Red Blood Cell Membrane) Acronym. Definition. ABRBC. Alcian Blue on Red Blood Cell Membrane. ...
Alcian yellow-toluidine blue Leung stain explanation free. What is Alcian yellow-toluidine blue Leung stain? Meaning of Alcian ... What does Alcian yellow-toluidine blue Leung stain mean? ... Looking for online definition of Alcian yellow-toluidine blue ... Alcian yellow-toluidine blue (Leung) stain. (redirected from Alcian yellow-toluidine blue Leung stain) Alcian yellow-toluidine ... Alcian yellow-toluidine blue Leung stain , definition of Alcian yellow-toluidine blue Leung stain by Medical dictionary https ...
... Hobbs, Carl carl.hobbs at kcl.ac.uk Thu Aug 23 13:32:13 CDT ... Perhaps Alcian blue at pH3 ( all acidic mucins) on one slide/section and Ab pH1 ( highly sulphated mucins only) on a sequential ... Previous message (by thread): [Histonet] Alcian Blue digested w Hyaluronidase - any alternatives? ... Next message (by thread): [Histonet] Alcian Blue digested w Hyaluronidase - any alternatives? ...
Find the best Labs and Diagnostic Centers for Alcian Blue Stain Urine Online in Bhubaneswar. View its pre-requirement, prices ...
The Alcian blue is used on tissue samples taken from the gastrointestinal tract and can be used to detect processes such as ... Alcian Blue Stain. View cart "Peripheral Blood Smear" has been added to your cart. ...
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Alcian Blue Pathology Outlines. Posted byadmin April 27, 2019. Thomas Edison Film Projector Each film was approximately 17 ... Alcian blue stain showed pools of mucin in the.. From the Departments of *Rheumatology and Clinical Immunology and †Trauma and ... Then we made thinly sliced dorsal sections of removed trachea and stained them with hematoxylin and eosin (HE) or alcian blue ( ... Importantly, at the age of 6 months, around 40% of CPK mice displayed cystic lesions classified as alcian blue/PAS-positive ...
... and Alcian Blue methods to demonstrate the full complement of tissue proteoglycans. ... Alcian Blue-PAS Stain Kit utilizes the properties of both the Periodic Acid Solution (PAS) ... The Alcian Blue-PAS Stain Kit utilizes the properties of both the Periodic Acid Solution (PAS) and Alcian Blue methods to ... The rationale of the technique is that by first staining all the acidic mucins with Alcian Blue, those acidic mucins which are ...
Hito Alcian Blue pH-2.5 OptimStain™ Kit is formulated for the staining of acid mucins, sulfated / carboxylated acid ... Hito Alcian Blue pH-2.5 OptimStain™ Kit has been tested extensively on the tissues from several species of animals and it is a ... Hito Alcian Blue pH-2.5 OptimStain™ Kit has been tested extensively on the tissues from several species of animals and it is a ... Hito Alcian Blue pH-2.5 OptimStain™ Kit is designed in a ready-to-use format and offers high quality, rapid and reproducible ...
Alcian Blue Binding Assays. The phosphomannan content was determined by measuring the binding of the cationic dye Alcian Blue ... Alcian Blue and found that none of the complemented transformants were capable of restoring wild-type levels of Alcian Blue ... Aliquots of 1 mL were pelleted, and cells suspended in 1 mL of 30 μg/mL Alcian Blue (in 0.02 M HCl) and incubated at room ... Complementation of this strain with MNN41 did not increase Alcian Blue binding, but complementation with either MNN42 or MNN47 ...
  • In addition to its wide use as a stain Alcian blue has also been used in other diverse applications e.g. gelling agent for lubricating fluids, modifiers for electrodes, charged coating agents etc. (wikipedia.org)
  • Use of alcian blue has historically been a popular staining method in histology especially for light microscopy in paraffin embedded sections and in semithin resin sections. (wikipedia.org)
  • The following parameters were analysed: effect on cell differentiation, as estimated by Q-PCR of transcription factors (TFs) and marker genes, or as mineralized/ALP positive surfaces, in vivo mineralization in SCID mice and de novo bone tissue production (histology), and proteoglycan positive surface (Alcian blue colouration). (intechopen.com)
  • Alcian blue or alcian blue (/ˈælʃən/) is any member of a family of polyvalent basic dyes, of which the Alcian blue 8G (also called Ingrain blue 1, and C.I. 74240, formerly called Alcian blue 8GX from the name of a batch of an ICI product) has been historically the most common and the most reliable member. (wikipedia.org)
  • ab150661 (Alcian Blue pH 1.0) staining strongly sulphated mucins (blue) in formalin fixed paraffin embedded human colon, counterstained with safranin o (pink). (abcam.com)
  • Click the button below to add the Alcian Blue (Mucins) Control Slides Paraffin Sections, 5 slides/pack to your wish list. (ihcworld.com)
  • The Monstral blue found to coat the inside of copper vessels used to process phthalic acid derivatives had led to the discovery of Phthalocyanine in 1907. (wikipedia.org)
  • Biochemists had used it to assay acid polysaccharides in urine since the 1960s for diagnosis of diseases like mucopolysaccharidosis but from 1970's, partly due to lack of availability of Alcian and partly due to length and tediousness of the procedure, alternative methods had to be developed e.g. (wikipedia.org)