Alcanivorax borkumensis is a marine bacterium that has ability to grow on limited substrates that mainly is alkanes. The ability to use wide range of hydrocarbons is advantage of this bacterium to other marine community bacteria. A. borkumensis have two genetic systems for alkane biodegradation. The First system is alkane hydroxylase (alk-B1and alk-B2) and the second system is cytochrome P450. Until now there is not any report on cloning of cytochrome P450 gene of A. borkumensis. In this study cytochrome P450 gene from A. borkumensis was cloned. At first P450 gene was cloned in pBluescript plasmid by blunt cloning and insertion was confirmed by colony PCR. Then P450 gene was cloned in PET-26 expression vector and finally, IPTG induced gene expression in E.coli BL-21 bacterium. It was shown that longer induction time led to more expression level. The recombinant enzyme has difference absorbance in 450 nm that confirmed the activity of this recombinant enzyme.
Nowadays, because of substantial use of petroleum-derived fuels the number and extension of hydrocarbon polluted terrestrial ecosystems is in growth worldwide. In remediation of aforementioned sites bioremediation still tends to be an innovative, environmentally attractive technology. Although huge amount of information is available concerning the hydrocarbon degradation potential of cultivable hydrocarbonoclastic bacteria little is known about the in situ long-term effects of petroleum derived compounds on the structure of soil microbiota. Therefore, in this study our aim was to determine the longterm impact of total petroleum hydrocarbons (TPHs), volatile petroleum hydrocarbons (VPHs), total alkyl benzenes (TABs) as well as of polycyclic aromatic hydrocarbons (PAHs) on the structure of bacterial communities of four different contaminated soil samples. Our results indicated that a very high amount of TPH affected positively the diversity of hydrocarbonoclastic bacteria. This finding was ...
Terpenoids, also known as isoprenoids, are a large class of natural products consisting of isoprene (C5) units. There are two biosynthetic pathways, the mevalonate pathway [MD:M00095] and the non-mevalonate pathway or the MEP/DOXP pathway [MD:M00096], for the terpenoid building blocks: isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). The action of prenyltransferases then generates higher-order building blocks: geranyl diphosphate (GPP), farsenyl diphosphate (FPP), and geranylgeranyl diphosphate (GGPP), which are the precursors of monoterpenoids (C10), sesquiterpenoids (C15), and diterpenoids (C20), respectively. Condensation of these building blocks gives rise to the precursors of sterols (C30) and carotenoids (C40). The MEP/DOXP pathway is absent in higher animals and fungi, but in green plants the MEP/DOXP and mevalonate pathways co-exist in separate cellular compartments. The MEP/DOXP pathway, operating in the plastids, is responsible for the formation of essential oil ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Marco, Antonio and Marín, Ignacio (2007) A general strategy to determine the congruence between a hierarchical and a non-hierarchical classification. BMC Bioinformatics, 8. 442 - ?. ISSN 1471-2105 McKew, BA and Coulon, F and Osborn, AM and Timmis, KN and McGenity, TJ (2007) Determining the identity and roles of oil-metabolizing marine bacteria from the Thames estuary, UK. Environmental Microbiology, 9 (1). 165 - 176. ISSN 1462-2912 McKew, BA and Coulon, F and Yakimov, MM and Denaro, R and Genovese, M and Smith, CJ and Osborn, AM and Timmis, KN and McGenity, TJ (2007) Efficacy of intervention strategies for bioremediation of crude oil in marine systems and effects on indigenous hydrocarbonoclastic bacteria. Environmental Microbiology, 9 (6). 1562 - 1571. ISSN 1462-2912 Meaburn, Emma and Harlaar, Nicole and Craig, Ian W and Schalkwyk, Leonard C and Plomin, Robert (2007) Genomewide gene expression profiles as a biological basis for non-shared environment: MZ twins discordant for reading ...
Biohazard level, growth media and temperature, gram stain, industrial applications and more information for Alcanivorax jadensis.
Compare 341 hotels in Malacca using 13893 real guest reviews. Earn free nights and get our Price Guarantee - booking has never been easier on Hotels.com!
Do you plant vegetables in your garden ? I have been trying to plant ladys finger (okra) in my little backyard in Malacca.
I have a buddy from Malacca who now live in SJ. He has acute diabetes.. :(
Someone told me drinking ladys fingers juice can lower the blood sugar and I recommended him to try. It works quite ok for him.. :)
He is a retiree. Diabetic medicine is not cheap (even before GST) and his children kind of dont take care of him much. You know vegetable is also not
ish ish, cant wait to NOT see those food pictures.. turn on ppls saliva but cannot fulfill one loorrr.. so better dun look at those food pics tat u sure will publish one loor.. hhehe actually.. frankly.. pls share with me.. can see better than nothing.. at least can fantasize.. :) ...
MALACCA: Former acting Penang CID chief P.R Gunarajan has no qualms being the oldest person to be called to the Bar of Malacca High Court.
There are 14 eight-letter words containing 3A and 2C: ALPACCAS BACCARAS BACCARAT ... CHAMPACA MACCABAW MALACCAS. Every word on this site can be used while playing scrabble. Create other lists, that begin with or end with letters of your choice.
Neue Hesperiden des indischen Archipels und Ost-Africas aus der Collection des Herrn H. Ribbe in Blasewitz-Dresden, gesammelt von den Herren: C. Ribbe auf Celebes, Java un den Aru-Inseln, K nstler auf Malacca (Perak); K hn auf West-Guinea (Jekar); Menger auf Ceylon Berl. ent. Z. 29 (2) : 225-232 ...
Straits Muslims: Diasporas of the Northern Passage Of The Straits of Malacca is an edited volume by six writers Wazir Jahan Karim, M.Ismail Marcinkowski, Syed Farid Alatas, Khoo Salma Nasution, Abdur-Razzaq Lubis and Rokiah Talib on the migration of Tamils, Arabs (Hadramis), Acehnese, Minang and other Muslim minorities from India, Yemen and Sumatra into the Northern port cities in the Straits of Malacca over the last two centuries.. The diasporas of Muslims into Southeast Asia in particular the ports of Penang, Aceh Malacca and Singapore was closely associated with the intensity of trade, social networks, domestication through marriage and political involvement in pre-colonial and colonial European history. This placed Muslim minorities at a considerable economic and political advantage above indigenous Muslim Malay and proto-Malay communities and empowered them to establish the foundations of Muslim civil societies based on the principles of charitable benevolence, mutual trust, cooperation and ...
Oceanospirillales merupakan ordo yang beraneka ragam jika dilihat dari sisi morfologi dan metabolismenya. Beberapa dapat tumbuh di tengah-tengah oksigen, sementara yang lain membutuhkan lingkungan yang anaerob.[2] Most Oceanospirillales are prefer or require high salt concentrations to grow.[2] Mereka ada di berbagai macam relung, tetapi Oceanospirillales memperoleh energi dengan mengolah berbagai produk organik. Semua bakteri Oceanospirillales bersifat motil kecuali untuk anggota genus Alcanivorax.[2] ...
Alkane-degrading bacteria have developed different ways to accommodate alkane oxidation. In order to regulate alkane oxidation in E. coli, genes encoding a regulatory mechanism controlled by alkane levels must be incorporated. High alkane levels can then be used to activate expression of specific genes involved in the alkane degradation. Two types of regulatory constructs will be created in the form of BioBricks to achieve this. The first construct proposed is based on a catabolic repression pathway encoded by the P.putida Gpo1 OCT plamid. The second mechanism makes use of a global regulator protein from E.coli which is known to bind to regions of promoters known to activate genes involved in the degradation of non-glucose carbon sources. The BioBricks including these mechanisms will be implemented in Escherichia coli K12 and characterized and evaluated on it regulating capabilities controlled by alkane levels. ...
This chapter focuses on the aerobic degradation of hydrocarbons, with an emphasis on alkanes, and the applications of enzymes involved in these processes for biocatalysis. Yeasts and fungi are often mentioned as alkane degraders, for example in connection with the production of single-cell proteins or organic acids and amino acids from hydrocarbons. Soluble and particulate mono-oxygenases are known to oxidize the same compounds, and some of the gene diversity detected with primers that amplify membrane-bound methane mono-oxygenase (pMMO) and soluble MMO (sMMO) genes may well be due to short-chain alkane-degrading bacteria instead of methanotrophs. The application of oxygenases in biocatalytic processes is more complicated than that of enzymes such as hydrolases, because of cofactor and oxygen requirements, the sensitive nature of many oxygenases, the toxicity of substrates and products to the biocatalyst, and the uptake of the lipophilic substrates. The best strain in this study was a hexane-degrading
Check prices and reviews of 3 quality Eczema & Psoriasis Treatment clinics in Malacca, rated 4.5 over 5 from 70 verified reviews by our community medical support network. View doctor profiles, clinic contact information and photos. All clinics verified by ministry of health Malaysia. Send an enquiry and get response fast - Updated Sep 2020
Terendak Military Cemetery, Malaysia Location The Terendak Military Cemetery is situated within the Terendak Camp, Malacca. The Camp is located on the West Coast, thirteen miles north of Malacca on the Malacca-Masjid Tanah Road. The camp at Malacca was built by the governments of Great Britain, Australia and New Zealand during 1957-1959 to house the 28th Commonwealth Infantry Brigade on its move from North Malaysia in 1959-1960.
Putrajaya was erected after 495 years the Malay Empire of Malacca collapsed in 1511 to the colonialists.The Malay Empire of Malacca was established by Parameswara, a Malay Prince in 1400 after he was driven out of Palembang, the capital of old Malay Empire of Srivijaya established around 200 AD century and perished to the Majapahit and cease to exist in 1400. ...
I love driving the Prado, for all the wrong reasons. Its got a clunky manual gearbox, a heavy clutch pedal, soft suspension that will squat and dive with every touch of the throttle and brake, not to mention roll when steering. Its precisely these that make it an engaging drive- its extremely difficult to drive really smoothly. And Ive mastered it. (At least I think so.) Last CNY, I drove my family along the back roads from Kuala Pilah to Malacca. Being smooth on roads sprinkled with fast straights, sharp hairpins and long blind curves was a challenge, and my parents remarked (its quite a rare occasion) that the ride was a gentle one (I was actually going quite fast ...
THE fasting month and hot weather proved to be no deterrent to Kolej Yayasan Saad students who decided to do something meaningful with their time by bringing cheer to the sick and the lonely at the Malacca General Hospital recently.
If you have time, Id appreciate your answers to this questionnaire: * In what ways is MACL superior to other CLs, MacScheme, other Schemes and lisps? * If you could change or add three things, what would they be? * What do you use MACL for? * Do you use the application builder? * Do you use it for interactive (graphical, point-and-click) interfaces? If so, does the interface have a good response? For a real application would it be necessary to recode some or all of it in C or Pascal? * If youve used MacScheme+Toolkit, and have still more time to indulge me, please answer the previous questions with respect to that product. Thanks, Patrick -- Patrick Logan, uunet!mntgfx!plogan Mentor Graphics Corp. 8500 SW Creekside, Beaverton, Oregon 97005-7191 ...
Apex has partnered with Mid America Clinical Labs (MACL) to offer antibody testing that helps determine if someone has been infected with COVID-19.
Plasmid pKS104 from Dr. Jay Keaslings lab contains the insert fadD (M3351), atfA and is published in Nature. 2010 Jan 28;463(7280):559-62. This plasmid is available through Addgene.
The Green Party is accusing a crown research institute of trying to censor a critical report into its genetic engineering research methods.
Literature on hydrocarbon degradation in extreme hypersaline media presents studies that point to a negative effect of salinity increase on hydrocarbonoclastic activity, while several others report an opposite tendency. Based on information available
Subdivisions Main articles: States and federal territories of Malaysia, Divisions of Malaysia, and Districts of Malaysia Perlis Kedah Penang Kelantan Terengganu Perak Selangor Negeri Sembilan Malacca Johor Pahang Sarawak Sabah Labuan Kuala Lumpur Putrajaya West Malaysia East Malaysia (Blue) States (Red) Federal Territories South China Sea Strait of Malacca Gulf of Thailand Sulu Sea Celebes Sea Brunei…
For the process of dabbing, you require low-tech materials for production of clean as well as flavored vapor. To start with dabbing all you need to acquire is a dab rig. In this instrument, water is filled which is inhaled by you in the form of vapor. Dabs are acquired as concentrate from Cannabinoids, more specifically CBD as well as THC by the help of carbon dioxide and butane.. A sticky and thick oil is produced which is called by several names viz. wax, butter, shatter, etc. THC component is highly present in dabs. The wax which is created during dabbing is very thick and needs to be cleaned daily or it creates a lot of hindrance during the process. Thus, consumers get easily addicted to it and they also suffer from severe hallucinations.. ...
The Alkanivore E.coli strain has been designed to carry the genes required for the conversion of medium (C5-C13) and long chain (C15-C36) alkanes. A general scheme for the oxidation of the alkanes is illustrated in figure 1. To create the alkane degradation constructs a number of genes encoding for alkane degradation enzymes were synthesized and combined with promoters and ribosome binding sites obtained from the BioBrick distribution plates. Combination of these genes resulted in the following BioBrick constructs (the intermediates have also been submitted to the registry). ...
Draft genome of a single cell of Smithella sp. (Smithella sp. F21), sorted from a methanogenic alkane-degrading culture using a microfluidic device, subjected to multi displacement amplification, sequenced using Illumina Mi-Seq and assembled de novo using CLC Genomics Workbench 7 ...
Serving the Inland Northwest since 1883. Spokesman.com provides breaking and in-depth coverage of Spokane area news, sports and community.
We are worlds apart but guess what, both our barbers use the same type of chairs :). They are now collectibles and can be bought at good price. A pub here use it for a contest where someone sits in it with the head back and a gallon of beer is poured down his throat. How ghastly. Such a great picture. If you change the man, I swear you can find such a barber shop in Malacca, Malaysia. ...
It was known to British sailors in the 18th and 19th centuries as the Caramata Passage. The strait was used as a route by the British fleet for its Invasion of Java (1811), sailing from the British base in Malacca to the island of Java, then part of the Dutch East Indies.[2] Initially, the British were unsure of the straits practicability, and hesitated between taking it or a northeast passage around the north of Borneo and through Makassar Strait.[14] A report by Sir Thomas Stamford Raffles, then a British officer in Malacca, recommended taking the Karimata route, which he considered "less dangerous than tedious" than the northeast passage during that time of year.[15] Raffles estimated that this journey would take the British fleet between a month and six weeks for "the fleet sailing in divisions".[15] The British Governor-General of India The Lord Minto decided to take Raffless suggestion over the objection of some naval officers.[16] The fleet, numbering about 100 vessels and transporting ...
I refer to the letter Filth in Kota Baru by Shocked. There is also the other kind of filth in Kota Baru that the casual visitor may not see. That is the vast amount of overflowing rubbish from uncollected garbage bins, rubbish in clogged drains, thousands of plastic bags, mineral water bottles, shampoo bottles, wrappers, tin cans, construction rubble all indiscriminately thrown and discarded all over Kota Baru and Kelantan. The kampung areas in this state are nowhere near like those in Malacca or other west coast state which are clean, tidy and well-kept. The Kelantan villages are singularly filthy with stagnant drains and decorated with rubbish strewn all over. Cows, goats and chicken regularly feed off the dump sites. Many of these are created literally overnight after ceramahs - political and religious - or after expos and numerous pasar malam (night markets). The tourist does not see all this because the main streets and the tourist places are kept clean to dupe visitors into believing that ...
Shi, Qiang, Petrick, Gert, Quack, Birgit, Marandino, Christa and Wallace, Douglas W.R. (2013) An experimental study of volatile halocarbon production in surface seawater . ...
A free platform for explaining your research in plain language, and managing how you communicate around it - so you can understand how best to increase its impact.
A number of Cham also fled across the sea to Malay Peninsula and as early as the 15th century, a Cham colony was established in Malacca. The Chams encountered Sunni Islam there as the Malacca Sultanate was officially Muslim since 1414. The King of Champa then became an ally of the Johor Sultanate; in 1594, Champa sent its military forces to fight alongside Johor against the Portuguese occupation of Malacca.[24] Between 1607 and 1676, one of the Champa kings converted to Islam and it became a dominant feature of Cham society. The Chams also adopted the Jawi alphabet.[26] Historical records in Indonesia showed the influence of Queen Dwarawati, a Muslim Princess from the Kingdom of Champa (Chams), toward her husband, Kertawijaya, the Seventh King of Majapahit Empire, so that the royal family of the Majapahit Empire eventually converted to Islam, which finally lead to the conversion to Islam of the entire region.[27][28][29] Chams Princess tomb can be found in Trowulan, the site of the capital of ...
Characterization of a pyrene-degrading Mycobacterium sp. strain CH-2.: Mycobacterium sp strain CH-2 was isolated from a manufactured gas plant contaminated with
By Kunle Obadina. Maritime resources are critical to maritime states because they harbour vital resources which include oil and gas, cobalt, manganese nodules among others. In this era of economic diversification drive in Nigeria, the Federal Government of Nigeria (FGN) need not look too far. Nigerias maritime resources could serve as major source of foreign reserve if properly harnessed. However, in order to benefit from the huge maritime resources, the FGN need to improve its efforts at addressing the security threats confronting Nigerias maritime domain. Threats associated with maritime environment as have been witnessed in the Straits of Hormuz, Malacca, Singapore, Gulf of Guinea (GoG) among others include piracy, poaching, and even terrorism. Therefore protection of the maritime environment is vital if Nigeria is to exploit these abundant sea resources as part of the government diversification drive toward enhancing national development.. In Nigeria, the Nigerian Navy (NN) alongside other ...
Unbeknownst to the Sultan, Light had acted without the approval of the East India Company when he promised military protection. When the Company failed to aid Kedah when it was attacked by Siam, the Sultan tried to retake the island in 1790. The attempt was unsuccessful, and the Sultan was forced to cede the island to the Company for an honorarium of 6,000Spanish dollars per annum. This was later increased to 10,000 dollars, with Province Wellesley on the mainland of the Malay Peninsula being added to Penang in 1800. An annual honorarium of 10,000 ringgits continues to this day be paid by the Malaysian Federal Government to the state of Kedah.. In 1826, Penang, along with Malacca and Singapore, became part of the Straits Settlements under the British administration in India, moving to direct British colonial rule in 1867. In 1946 it became part of the Malayan Union, before becoming in 1948 a state of the Federation of Malaya, which gained independence in 1957 and became Malaysia in 1963.. The ...
With his characteristic warmth, inventiveness and brilliant wit, Alexander McCall Smith gives us more of the gloriously entertaining comings and goings at 44 Scotland Street, the Edinburgh townhouse. Six-year-old prodigy Bertie perseveres in his heroic struggle for truth and balanced good sense against his insufferable mother and her crony, the psychotherapist Dr Fairbairn, going as far as to make a short-lived bid for freedom on a trip to Paris with the Edinburgh youth orchestra. Domenica sets off on an anthropological odyssey with pirates in the Malacca Straits, while Pat attracts several handsome admirers, including a toothsome suitor named Wolf. And Big Lou, eternal source of coffee and good advice to her friends, has love, heartbreak and erstwhile boyfriend Eddies misdemeanours on her own mind.show more ...
Malaysian AIDS HIV news archive and resources by Utopia in Penang, Lankawi, Borneo, Johor Johore Bahru Bharu, Kedah Keda, Kota Kinabalu, Kuala lumpur, Kuantan, Kuching, Malacca, Petaling Jaya, Tawau, Sabah, Selangor
Microbial interactions have a key role in global geochemical cycles. Although we possess significant knowledge about the general biochemical processes occurring in microbial communities, we are often unable to decipher key functions of individual microorganisms within the environment in part owing to the inability to cultivate or study them in isolation. Here, we circumvent this shortcoming through the use of single-cell genome sequencing and a novel low-input metatranscriptomics protocol to reveal the intricate metabolic capabilities and microbial interactions of an alkane-degrading methanogenic community. This methanogenic consortium oxidizes saturated hydrocarbons under anoxic conditions through a thus-far-uncharacterized biochemical process. The genome sequence of a dominant bacterial member of this community, belonging to the genus Smithella, was sequenced and served as the basis for subsequent analysis through metabolic reconstruction. Metatranscriptomic data generated from less than 500 ...
Sultan Hussein Mohamed Shah (b. 1776-d. 5 September 1835, Malacca) or Tengku Long (or Sulong, which means eldest in Malay) or Tengku Hussein, was the eldest son of Sultan Mahmud Shah (b. 1761 - d. 14 January 1811, Lingga), the last Sultan of the Johore-Riau-Lingga empire. As the eldest son, Tengku Hussein was customarily the rightful heir but the throne went instead to his younger brother Tengku Abdul Rahman. Capitalising on the succession dispute, the British reinstated Hussein as the Sultan of Johore as part of their strategy to gain a foothold in the Far East trade routes.
General Information: An alkane-degrading sulfate-reducing Gram-negative bacterium isolated from the saline water phase of an oil-water separator from a northern German oil field. Desulfococcus oleovorans, originally designated Desulfobacterium oleovorans, was isolated using hexadecane as a carbon source and has been used to determine the mechanism of anaerobic hydrocarbon degradation. ...
Expression of MEPCE (BCDIN3, FLJ20257, MePCE) in esophagus tissue. Antibody staining with HPA051587 and CAB026384 in immunohistochemistry.
From the Indian point of view, all these Chinese development projects in the region are part of the larger Chinese plan to encircle India. For Indian military analysts and policy makers, the Chinese are everywhere in the region, including the Indian Ocean, which India has traditionally considered as its sphere of influence. The encirclement of India with ports, also known as the string of pearls, can be looked at from another perspective. Chinese are trying to safeguard their national interest and protect its economy. Chinas dependence on imported oil and natural gas has led it to think of possible openings in this part of the world, rather than depending completely on the Malacca Strait for the passage of its vessels. They are trying to protect their back. In case of a future conflict with the US, China does not want the US to be successful in blockading its shipping lane. The reason behind developing ports in the Indian Ocean is to ensure continued oil and energy flow to China. It is wrong ...
Now I try to learn how to create interesting videos. The following is one of my first few attempts to create video with interest. This video was filmed by me at at the 25th Anniversary of the Nonyas and Babas at Malacca in late 2012. There were around 700 participants from Malaysia, Singapore, Indonesia, Thailand and Australia. I am not a Peranakan (Straits-born Chinese adopting the Malay culture), Instead of sitting down with strangers making small talk, I took my camera to the dance floor and tried to do some videos. One video may be of interest to viewers ...
The largest Sikh festival in the world outside Punjab, which commemorates the death anniversary of a revered saint, will be held in the Malaysian state of Malacca next week.
This Histri was built automatically but not manually verified. As a consequence, the Histri can be incomplete or can contain errors ...