A genus of gram-negative, aerobic, motile bacteria that occur in water and soil. Some are common inhabitants of the intestinal tract of vertebrates. These bacteria occasionally cause opportunistic infections in humans.
The type species of gram negative bacteria in the genus ALCALIGENES, found in soil. It is non-pathogenic, non-pigmented, and used for the production of amino acids.
A species of gram-negative bacteria in the genus PSEUDOMONAS. It cannot utilize FRUCTOSE; GLUCOSE; or MALTOSE for energy.
Benzoic acid or benzoic acid esters substituted with one or more chlorine atoms.
An enzyme found in bacteria. It catalyzes the reduction of FERREDOXIN and other substances in the presence of molecular hydrogen and is involved in the electron transport of bacterial photosynthesis.
A genus of gram-negative, aerobic, rod-shaped bacteria widely distributed in nature. Some species are pathogenic for humans, animals, and plants.
An herbicide with irritant effects on the eye and the gastrointestinal system.
A genus of gram-negative, strictly aerobic, non-spore forming rods. Soil and water are regarded as the natural habitat. They are sometimes isolated from a hospital environment and humans.
Elimination of ENVIRONMENTAL POLLUTANTS; PESTICIDES and other waste using living organisms, usually involving intervention of environmental or sanitation engineers.
A bacterial protein from Pseudomonas, Bordetella, or Alcaligenes which operates as an electron transfer unit associated with the cytochrome chain. The protein has a molecular weight of approximately 16,000, contains a single copper atom, is intensively blue, and has a fluorescence emission band centered at 308nm.
Polymers of organic acids and alcohols, with ester linkages--usually polyethylene terephthalate; can be cured into hard plastic, films or tapes, or fibers which can be woven into fabrics, meshes or velours.
The type species of gram negative, aerobic bacteria in the genus ACHROMOBACTER. Previously in the genus ALCALIGENES, the classification and nomenclature of this species has been frequently emended. The two subspecies, Achromobacter xylosoxidans subsp. denitrificans and Achromobacter xylosoxidans subsp. xylosoxidans are associated with infections.
A group of enzymes that oxidize diverse nitrogenous substances to yield nitrite. (Enzyme Nomenclature, 1992) EC 1.
Aminobenzenesulfonic acids. Organic acids that are used in the manufacture of dyes and organic chemicals and as reagents.
The functional hereditary units of BACTERIA.
A product of fermentation. It is a component of the butanediol cycle in microorganisms. In mammals it is oxidized to carbon dioxide.
A trace element with the atomic symbol Ni, atomic number 28, and atomic weight 58.69. It is a cofactor of the enzyme UREASE.
Salts and esters of hydroxybutyric acid.
Non-heme iron-containing enzymes that incorporate two atoms of OXYGEN into the substrate. They are important in biosynthesis of FLAVONOIDS; GIBBERELLINS; and HYOSCYAMINE; and for degradation of AROMATIC HYDROCARBONS.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Derivatives of adipic acid. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain a 1,6-carboxy terminated aliphatic structure.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
A group of 1,2-benzenediols that contain the general formula R-C6H5O2.
A gram-negative, facultatively chemoautotrophic bacterium, formerly called Wautersia eutropha, found in water and soil.
Infections caused by bacteria that show up as pink (negative) when treated by the gram-staining method.
Oxidases that specifically introduce DIOXYGEN-derived oxygen atoms into a variety of organic molecules.
A family of gram-negative, aerobic, non-spore forming rods or cocci. Well known genera include ACHROMOBACTER; ALCALIGENES; and BORDETELLA.
Salts and esters of gentisic acid.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Salts of chromic acid containing the CrO(2-)4 radical.
The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Proteins found in any species of bacterium.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
The first chemical element in the periodic table. It has the atomic symbol H, atomic number 1, and atomic weight [1.00784; 1.00811]. It exists, under normal conditions, as a colorless, odorless, tasteless, diatomic gas. Hydrogen ions are PROTONS. Besides the common H1 isotope, hydrogen exists as the stable isotope DEUTERIUM and the unstable, radioactive isotope TRITIUM.
A parasexual process in BACTERIA; ALGAE; FUNGI; and ciliate EUKARYOTA for achieving exchange of chromosome material during fusion of two cells. In bacteria, this is a uni-directional transfer of genetic material; in protozoa it is a bi-directional exchange. In algae and fungi, it is a form of sexual reproduction, with the union of male and female gametes.
DNA elements that include the component genes and insertion site for a site-specific recombination system that enables them to capture mobile gene cassettes.
Substances that reduce the growth or reproduction of BACTERIA.
The genomic analysis of assemblages of organisms.
The ability of bacteria to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
The ability of microorganisms, especially bacteria, to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).

Analysis of the nitrous oxide reduction genes, nosZDFYL, of Achromobacter cycloclastes. (1/705)

The structural gene, nosZ, for the monomeric N2O reductase has been cloned and sequenced from the denitrifying bacterium Achromobacter cycloclastes. The nosZ gene encodes a protein of 642 amino acid residues and the deduced amino acid sequence showed homology to the previously derived sequences for the dimeric N2O reductases. The relevant DNA region of about 3.6 kbp was also sequenced and found to consist of four genes, nosDFYL based on the similarity with the N2O reduction genes of Pseudomonas stutzeri. The gene product of A. cycloclastes nosF (299 amino acid residues) has a consensus ATP-binding sequence, and the nos Y gene encodes a hydrophobic protein (273 residues) with five transmembrane segments, suggesting the similarity with an ATP-binding cassette (ABC) transporter which has two distinct domains of a highly hydrophobic region and ATP-binding sites. The nosL gene encodes a protein of 193 amino acid residues and the derived sequence showed a consensus sequence of lipoprotein modification/processing site. The expression of nosZ gene in Escherichia coli cells and the comparison of the translated sequences of the nosDFYL genes with those of bacterial transport genes for inorganic ions are discussed.  (+info)

Nitrate removal in closed-system aquaculture by columnar denitrification. (2/705)

The columnar denitrification method of nitrate-nitrogen removal from high-density, closed system, salmonid aquaculture was investigated and found to be feasible. However, adequate chemical monitoring was found to be necessary for the optimization and quality control of this method. When methanol-carbon was not balanced with inlet nitrate-nitrogen, the column effluent became unsatisfactory for closed-system fish culture due to the presence of excess amounts of nitrite, ammonia, sulfide, and dissolved organic carbon. Sulfide production was also influenced by column maturity and residence time. Methane-carbon was found to be unsatisfactory as an exogenous carbon source. Endogenous carbon could not support high removal efficiencies. Freshwater columns adpated readily to an artificial seawater with a salinity of 18% without observable inhibition. Scanning electron microscopy revealed that the bacterial flora was mainly rod forms with the Peritricha (protozoa) dominating as the primary consumers. Denitrifying bacteria isolated from freshwater columns were tentatively identified as species of Pseudomonas and Alcaligenes. A pilot plant column was found to behave in a manner similar to the laboratory columns except that nitrite production was never observed.  (+info)

Biochemical characterization and solution structure of nitrous oxide reductase from Alcaligenes xylosoxidans (NCIMB 11015). (3/705)

Nitrous oxide reductase (N2OR) is the terminal enzyme involved in denitrification by microbes. No three-dimensional structural information has been published for this enzyme. We have isolated and characterised N2OR from Alcaligenes xylosoxidans (AxN2OR) as a homodimer of M(r) 134,000 containing seven to eight copper atoms per dimer. Comparison of sequence and compositional data with other N2ORs suggests that AxN2OR is typical and can be expected to have similar domain folding and subunit structure to other members of this family of enzymes. We present synchrotron X-ray-scattering data, analysed using a model-independent method for shape restoration, which gave a approximately 20 A resolution structure of the enzyme in solution, providing a glimpse of the structure of any N2OR and shedding light on the molecular architecture of the molecule. The specific activity of AxN2OR was approximately 6 mumol of N2O reduced.min-1. (mg of protein)-1; N2OR activity showed both base and temperature activation. The visible spectrum exhibited an absorption maximum at 550 nm with a shoulder at 635 nm. On oxidation with K3Fe(CN)6, the absorption maximum shifted to 540 nm and a new shoulder at 480 nm appeared. Reduction under anaerobic conditions resulted in the formation of an inactive blue form of the enzyme with a broad absorption maximum at 650 nm. As isolated, the enzyme shows an almost featureless EPR spectrum, which changes on oxidation to give an almost completely resolved seven-line hyperfine signal in the gII region, g = 2.18, with AII = 40 G, consistent with the enzyme being partially reduced as isolated. Both the optical and EPR spectra of the oxidized enzyme are characteristic of the presence of a CuA centre.  (+info)

PCR detection of genes encoding nitrite reductase in denitrifying bacteria. (4/705)

Using consensus regions in gene sequences encoding the two forms of nitrite reductase (Nir), a key enzyme in the denitrification pathway, we designed two sets of PCR primers to amplify cd1- and Cu-nir. The primers were evaluated by screening defined denitrifying strains, denitrifying isolates from wastewater treatment plants, and extracts from activated sludge. Sequence relationships of nir genes were also established. The cd1 primers were designed to amplify a 778 to 799-bp region of cd1-nir in the six published sequences. Likewise, the Cu primers amplified a 473-bp region in seven of the eight published Cu-nir sequences. Together, the two sets of PCR primers amplified nir genes in nine species within four genera, as well as in four of the seven sludge isolates. The primers did not amplify genes of nondenitrifying strains. The Cu primers amplified the expected fragment in all 13 sludge samples, but cd1-nir fragments were only obtained in five samples. PCR products of the expected sizes were verified as nir genes after hybridization to DNA probes, except in one case. The sequenced nir fragments were related to other nir sequences, demonstrating that the primers amplified the correct gene. The selected primer sites for Cu-nir were conserved, while broad-range primers targeting conserved regions of cd1-nir seem to be difficult to find. We also report on the existence of Cu-nir in Paracoccus denitrificans Pd1222.  (+info)

The blue copper-containing nitrite reductase from Alcaligenes xylosoxidans: cloning of the nirA gene and characterization of the recombinant enzyme. (5/705)

The nirA gene encoding the blue dissimilatory nitrite reductase from Alcaligenes xylosoxidans has been cloned and sequenced. To our knowledge, this is the first report of the characterization of a gene encoding a blue copper-containing nitrite reductase. The deduced amino acid sequence exhibits a high degree of similarity to other copper-containing nitrite reductases from various bacterial sources. The full-length protein included a 24-amino-acid leader peptide. The nirA gene was overexpressed in Escherichia coli and was shown to be exported to the periplasm. Purification was achieved in a single step, and analysis of the recombinant Nir enzyme revealed that cleavage of the signal peptide occurred at a position identical to that for the native enzyme isolated from A. xylosoxidans. The recombinant Nir isolated directly was blue and trimeric and, on the basis of electron paramagnetic resonance spectroscopy and metal analysis, possessed only type 1 copper centers. This type 2-depleted enzyme preparation also had a low nitrite reductase enzyme activity. Incubation of the periplasmic fraction with copper sulfate prior to purification resulted in the isolation of an enzyme with a full complement of type 1 and type 2 copper centers and a high specific activity. The kinetic properties of the recombinant enzyme were indistinguishable from those of the native nitrite reductase isolated from A. xylosoxidans. This rapid isolation procedure will greatly facilitate genetic and biochemical characterization of both wild-type and mutant derivatives of this protein.  (+info)

Transcriptional organization of the czc heavy-metal homeostasis determinant from Alcaligenes eutrophus. (6/705)

The Czc system of Alcaligenes eutrophus mediates resistance to cobalt, zinc, and cadmium through ion efflux catalyzed by the CzcCB2A cation-proton antiporter. DNA sequencing of the region upstream of the czcNICBADRS determinant located on megaplasmid pMOL30 revealed the 5' end of czcN and a gene for a MgtC-like protein which is transcribed in the orientation opposite that of czc. Additional open reading frames upstream of czc had no homologs in the current databases. Using oligonucleotide-probed Northern blotting experiments, a 500-nucleotide czcN message and a 400-nucleotide czcI message were found, and the presence of 6, 200-nucleotide czcCBA message (D. Van der Lelie et al., Mol. Microbiol. 23:493-503, 1997) was confirmed. Induction of czcN, czcI, czcCBA, and czcDRS followed a similar pattern: transcription was induced best by 300 microM zinc, less by 300 microM cobalt, and only slightly by 300 microM cadmium. Reverse transcription-PCR gave evidence for additional continuous transcription from czcN to czcC and from czcD to czcS, but not between czcA and czcD nor between czcS and a 131-amino-acid open reading frame following czcS. The CzcR putative response regulator was purified and shown to bind in the 5' region of czcN. A reporter strain carrying a czcNIC-lacZ-czcBADRS determinant on plasmid pMOL30 was constructed, as were DeltaczcR and DeltaczcS mutants of this strain and of AE128(pMOL30) wild type. Experiments on (i) growth of these strains in liquid culture containing 5 mM Zn2+, (ii) induction of the beta-galactosidase in the reporter strains by zinc, cobalt, and cadmium, and (iii) cDNA analysis of czcCBA mRNA synthesis under inducing and noninducing conditions showed that the CzcRS two-component regulatory system is involved in Czc regulation.  (+info)

Re-evaluation of the primary structure of Ralstonia eutropha phasin and implications for polyhydroxyalkanoic acid granule binding. (7/705)

Sequence analysis of several cDNAs encoding the phasin protein of Ralstonia eutropha indicated that the carboxyl terminus of the resulting derived protein sequence is different from that reported previously. This was confirmed by: (1) sequencing of the genomic DNA; (2) SDS-PAGE and peptide analysis of wild-type and recombinant phasin; and (3) mass spectrometry of wild-type phasin protein. The results have implications for the model proposed for the binding of this protein to polyhydroxyalkanoic acid granules in the bacterium.  (+info)

Phospholipid bound to the flavohemoprotein from Alcaligenes eutrophus. (8/705)

The structurally characterized flavohemoprotein from Alcaligenes eutrophus (FHP) contains a phospholipid-binding site with 1-16 : 0-2-cyclo-17 : 0-diacyl-glycerophospho-ethanolamine and 1-16 : 0-2-cyclo-17 : 0-diacyl-glycerophospho-glycerol as the major occupying compounds. The structure of the phospholipid is characterized by its compact form, due to the -sc/beta/-sc conformation of the glycerol and the nonlinear arrangement of the sn-1- and sn-2-fatty acid chains. The phospholipid-binding site is located adjacent to the heme molecule at the bottom of a large cavity. The fatty acid chains form a large number of van der Waal's contacts with nonpolar side chains, whereas the glycerophosphate moiety, which points towards the entrance of the channel, is linked to the protein matrix by polar interactions. The thermodynamically stable globin module of FHP, obtained after cleaving off the oxidoreductase module, also contains the phospholipid and can therefore be considered as a phospholipid-binding protein. Single amino acid exchanges designed to decrease the lipid-binding site revealed both the possibility of blocking incorporation of the phospholipid and its capability to evade steric barriers. Conformational changes in the phospholipid can also be induced by binding heme-ligating compounds. Phospholipid binding is not a general feature of flavohemoproteins, because the Escherichia coli and the yeast protein exhibit less and no lipid affinity, respectively.  (+info)

Siderophore production in response to iron limitation was observed in Alcaligenes eutrophus CH34, and the corresponding siderophore was named alcaligin E. Alcaligin E was characterized as a phenolate-type siderophore containing neither catecholate nor hydroxamate groups, Alcaligin E promoted the growth of siderophore-deficient A. eutrophus mutants under iron-restricted
Role of arginine residues of D-aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6.: To investigate the role of arginine in the folding of d-amin
Colonization by Alcaligenes xylosoxidans in children with cystic fibrosis: a retrospective clinical study conducted by means of molecular epidemiological investigation
Gentaur molecular products has all kinds of products like :search , AbD \ GOAT ANTI ALCALIGENES SPP CHOLINE OXIDASE, Product Type Polyclonal Antibody, Specificity CHOLINE OXIDASE, Target Species Bacterial, Host Goat, Format Purified, Isotypes Polyclonal IgG, Applicati \ 2080-5050 for more molecular products just contact us
A sandy loam soil near field capacity moisture content (psi = -0.050 MPa) or air dried (psi = -300 MPa) was inoculated with about 3 x 10(7) CFU of Enterobacter cloacae JP120 and Alcaligenes eutrophus AEO106(pRO101) per g and incubated in 40-g portions at 17 degrees C in closed or open Erlenmeyer flasks. In the field-moist soil, selective plating, direct viable counts, and DNA hybridization showed only minor changes in the numbers of E. cloacae and A. eutrophus cells with time (14 days), and the results obtained with the three detection methods generally agreed. In the air-dried soil, the majority of both bacteria were found as intact DNA-carrying cells that were neither culturable nor viable by the methods employed in this study. The numbers of culturable E. cloacae and A. eutrophus cells dropped to 10(5) and 10(2) CFU/g, respectively, 2 h after inoculation. Direct viable counts showed that only about 1% of the cells detected by immunofluorescence microscopy were viable, but a fraction of viable ...
1ET7: Catalytic roles for two water bridged residues (Asp-98 and His-255) in the active site of copper-containing nitrite reductase.
1l9s: Directing the mode of nitrite binding to a copper-containing nitrite reductase from Alcaligenes faecalis S-6: characterization of an active site isoleucine.
TWENTY FIVE Aspergillus isolates were screened from Giza Governorate and Saint Catherine Protectorate soils in Egypt. The antimicrobial activity of the crude extracts was tested against two Gram positive bacteria (Bacillus subtilis NRRL-B-4219, Staphylococcus aureus ATCC29213), four Gram negative bacteria (Alcaligenes faecalis B-170, Escherichia coli ATCC25922, Klebsiella pneumoniae ATCC10131, Pseudomonas aeruginosa ATCC27953), and one yeast (Candida albicans ATCC10231). The antioxidant activity using free radical scavenging model was assayed for the crude extracts. The antitumor activity for all of crude extracts was determined against HCT116 (Colon carcinoma cell line), HEPG2 (Liver carcinoma cell line), and MCF-7 (Breast carcinoma cell line). Aspergillus nomius was the most potent fungal species accordingly, it was chosen for bioactivity assay. Identification of this species was further confirmed at the molecular level based on nuclear ribosomal DNA 18s identities. An accession number, LC199488, was
1GS6: Biochemical and Crystallographic Studies of the met144Ala, Asp92Asn and His254Phe Mutants of the Nitrite Reductase from Alcaligenes Xylosoxidans Provide Insight Into the Enzyme Mechanism.
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Eight strains of hydrogen bacteria belonging to the genera Hydrogenomonas, Pseudomonas, Nocardia, and to coryneform bacteria have been analyzed with respect to the cytochrome patterns. All strains...
Alcaligenes Polysaccharides are manufactured by Hakuto under the tradename Alcasealan, and probably distributed in Europe by Nagase. It is described as a natural polysaccharides produced from fermentation. The polymer chain of Alcasealan forms a three-dimension network to hold free water as well as binding it. This 3D network has a unique characteristic which forms a moisturising film on the skin with unique lightweight texture. Alcasealan is widely used as emulsion and dispersion stabiliser; it also helps to suspense powders and glitters in cosmetics formulations. ...
This is a water gelling agent made from a Gram-negative bacteria, similar to xanthan gum.. A 0.001% concentration can thicken up a product quite nicely, which is good because it costs more than $10 000 for a kilo.. Beyond thickening water, it also has moisturizing properties, similar to hyaluronic acid.. Is it better or worth the money? Im not sure - but if the popularity of this ingredient increases, the cost will go down. Much of the cost is the low yield from production. You can read more about how its produced here.. Japanese and Korean suppliers tend to have many unique and interesting raw materials, from my experience.. Its found in a few commercial products, which are very luxury and expensive (as is to be expected).. ...
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Achromobacter species derived from the sputa of CF patients are garnering increasing attention. Indeed, improvements in diagnostic techniques enabling the correct distinction of these species from other CF pathogens, such as P. aeruginosa, and selective antimicrobial pressures may have contributed to their initial recognition (19, 23), although their rates did not appear to be changing over the last decade (7). Our study is the first to report epidemiological and clinical outcome data for CF patients infected with Achromobacter species in a North American cohort.. The cumulative prevalence of Achromobacter isolation of 11% in our center is comparable to those previously reported for small centers, which ranged from 5 to 29% (11, 12), although this spans 3 decades, making the incidence of infection very low. While others have reported that older patients and patients with greater lung disease burden appear to be predisposed to infection with Achromobacter species (11, 18), we did not observe any ...
Achromobacter sp. AO22 (formerly Alcaligenes sp. AO22), a bacterial strain isolated from a lead-contaminated industrial site in Australia, was previously found to be resistant to moderate to high levels of mercury, copper and other heavy metals. However, the nature and location of the genetic basis for mercuric ion resistance in this strain, had not been previously identified. Achromobacter sp. AO22 contains a functional mer operon with all four essential genes (merRTPA) and shows |99% DNA sequence identity to that of Tn501. The mer operon was present on a transposon, designated TnAO22, captured by introducing a broad-host-range IncP plasmid into Achromobacter sp. AO22 and subsequently transferring it to E. coli recipients. The transposition frequency of TnAO22 was 10-2 to 10-3 per target plasmid transferred. Analysis of TnAO22 sequence revealed it belonged to the Tn21 subgroup of the Tn3 superfamily of transposons, with the transposition module having |99% identity with Tn5051 of a Pseudomonas putida
2-Aminobenzenesulphonic acid (2AS) is degraded by Alcaligenes sp. strain O-1 via a previously detected but unidentified intermediate. A mutant of strain O-1 was found to excrete this intermediate, which was isolated and identified by m.s., 1H- and 13C-n.m.r. as 3-sulphocatechol (3SC). Proteins from cell extracts of strain O-1 were separated by anion-exchange chromatography. A multicomponent oxygenase was observed to convert 1 mol each of NADH, O2 and 2AS into 1 mol each of 3SC, NH3 and NAD+. The enzyme presumably catalysed formation of the ring of a 2-amino-2,3-diol moiety, and elimination in the amino group led to a rearomatization. 3SC was further degraded via meta ring cleavage, which could be prevented by inactivation of the 3-sulphocatechol-2,3-dioxygenase (3SC23O) with 3-chlorocatechol. In Tris buffer, the separated 3SC23O catalysed the reaction of 1 mol each of 3SC and O2 involving a transient yellow intermediate, and release of 1 mol of sulphite and two organic products. The major ...
This large outbreak (N = 12) of A. xylosoxidans bloodstream infections was caused by 1 strain, which was also isolated from CVC biofilm. Symptoms of bloodstream infection probably occurred when flushes detached bacteria from the CVC biofilm. The prospective study found that 3 (10%) of 29 patients had A. xylosoxidans-positive blood cultures. Our case-control and prospective studies support the association of A. xylosoxidans bloodstream infection and CVCs, and our molecular biologic studies confirm A. xylosoxidans biofilm of the same outbreak strain on a CVC. A. xylosoxidans outbreaks reported to date have been associated with healthcare and contamination of hospital products (1,2,5,12-14), but none occurred in an outpatient setting.. The cause of this outbreak most likely was the use of contaminated multidose vials of heparin or saline flushes, leading to the formation of an A. xylosoxidans biofilm on CVCs. Case-patient 1 had been hospitalized from late October through early November at Hospital ...
Alcaligenes faecalis subsp. faecalis ATCC ® 8750™ Designation: 16 TypeStrain=True Application: Produces nitrilase Quality control strain Quality control strain for Autobac products
Alcaligenes faecalis subsp. faecalis ATCC ® 35655™ Designation: LRA 41 02 82 TypeStrain=False Application: Quality control strain Quality control strain for API products
The nickel resistance determinant ncrABCY was identified in Leptospirillum ferriphilum UBK03. Within this operon, ncrA and ncrC encode two membrane proteins that form an efflux system, and ncrB encodes NcrB, which belongs to an uncharacterized family (DUF156) of proteins. How this determinant is regulated remains unknown. Our data indicate that expression of the nickel resistance determinant is induced by nickel. The promoter of ncrA, designated pncrA, was cloned into the promoter probe vector pPR9TT, and co-transformed with either a wild-type or mutant nickel resistance determinant. The results revealed that ncrB encoded a transcriptional regulator that could regulate the expression of ncrA, ncrB, and ncrC. A GC-rich inverted repeat sequence was identified in the promoter pncrA. Electrophoretic mobility shift assays (EMSAs) and footprinting assays showed that purified NcrB could specifically bind to the inverted repeat sequence of pncrA in vitro; this was confirmed by bacterial one-hybrid analysis.
A Megaplasmid-Borne Anaerobic Ribonucleotide Reductase in Alcaligenes eutrophus H16: The conjugative 450-kb megaplasmid pHG1 is essential for the anaerobic grow
The biopharmaceutical industry needs faster and more efficient development of new drugs and their market introduction as well as shorter process development times for both upstream and downstream operations. It has become more commonplace to use high-throughput development techniques to save time (1). Development is also sped up by applying platform technologies based on the unsurpassed selectivity of protein A resins (2,3,4,5,6), which is the foundation for downstream processing of monoclonal antibodies (MAbs). This is the second of two articles…. ...
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In nature a significant part of the microbial activity is concentrated at or near oxic/anoxic interfaces, where oxygen concentrations are often low. Bacteria possessing different kinetic characteristics for oxygen and employing distinct metabolic pathways for the degradation of (halo)aromatic substrates for which oxygen is needed as co-substrate may have to compete with each other in such environments. In this study the competitiveness of Pseudomonas sp. strain A3 relative to Alcaligenes sp. strain L6 was tested in batch and in continuous cultures. While both of these strains are able to metabolise 3-chlorobenzoate (3CBA), the former was isolated under air saturating conditions and employs the catechol pathway, whereas the latter was isolated under reduced partial pressures of oxygen and was capable of metabolising 3CBA via the gentisate pathway. Competition experiments in batch culture resulted in pure cultures of Pseudomonas sp. strain A3 under air saturating conditions. However, if reduced ...
It is generally assumed that tethering enhances rates of electron harvesting and delivery to active sites in multidomain enzymes by proximity and sampling mechanisms. Here, we explore this idea in a tethered 3-domain, trimeric copper-containing nitrite reductase. By reverse engineering, we find that …
Background and Aims: Food is of paramount importance to the sustenance of human health, on that basis this study was initiated with a view to determining bacterial agents associated with the vended foods and to investigating antibacterial resistance of the isolates. Methods: Twelve food samples from vendors were randomly collected from ten locations within Lagos environs. The food samples were analyzed bacteriologically by standard methods. The isolates were identified and subjected to antimicrobial susceptibility testing using standard procedures. Results: The total plate counts of bacteria recorded was between 2.98 × 103 cfu/g in Jollofrice and 4.09 × 104 cfu/g in Salad. Seventy-six bacterial isolates made up of six genera; Escherichia coli, Salmonella Typhimurium, Alcaligenes spp., Klebsiella pneumoniae, Enterobacter spp., Serratia marcensens and Proteus spp. were identified. Most of the bacterial isolates were moderately sensitive to cotrimoxazole, nalidixic acid, amoxicillin and nitrofurantion
Knowledge and management of aquatic ecosystems (formerly Bulletin Français de la Pèche et de la Pisciculture), an international journal on freshwater ecosystem
strict aerobe by thioglyoclate test, negative carb fermentation, positive citrate, negative indole, positive urease, catalase positive, gram negative rod,positive decarboxylation tests......I think I have Alcaligenes faecalis or pseudomonas aerugenosa. Motility was positive from the deep stab. I think my test results match those organism except for the positive urease ...
bacteria HoxJ protein: putative histidine protein kinase and molecular hydrogen sensor; GenBank U82565 (Alcaligenes hydrogenophilus)
Dodgson, K.S., Spencer, B. and Williams, K. (1956). „Studies on sulphatases. 13. The hydrolysis of substituted phenyl sulphates by the arylsulphatase of Alcaligenes metacaligenes. Biochem. J. 64: 216-221. PMID 13363831 ...
Achromobacter xylosoxidans (formerly Alcaligenes xylosoxidans) is a Gram-negative, aerobic, oxidase and catalase-positive, motile bacterium with peritrichous flagella, from the genus Achromobacter. It is generally found in wet environments. Achromobacter xylosoxidans can cause infections such as bacteremia, especially in patients with cystic fibrosis. In 2013, the complete genome of an A. xylosoxidans strain from a patient with cystic fibrosis was sequenced. A. xylosoxidans is a Gram-negative rod that does not form spores. It is motile, with peritrichous flagella that distinguish it from Pseudomonas species, and is oxidase-positive, catalase-positive, and citrate-positive. It is urease and indole-negative. It produces acid oxidatively from xylose, but not from lactose, maltose, mannitol, or sucrose. It grows well on MacConkey agar and other inhibitory growth media such as deoxycholate, Salmonella-Shigella, and nalidixic acid-cetrimide agars. It is usually resistant to a variety of antibiotics ...
TY - JOUR. T1 - Class 1 integrons and tetracycline resistance genes in Alcaligenes, Arthrobacter, and Pseudomonas spp. isolated from pigsties and manured soil. AU - Agersø, Yvonne. AU - Sandvang, Dorthe. PY - 2005. Y1 - 2005. N2 - The presence of tetracycline resistance (Tc-r) genes and class I integrons (in-1), and their ability to cotransfer were investigated in Tc-r gram-negative (185 strains) and gram-positive (72 strains) bacteria from Danish farmland and pigsties. The isolates belonged to the groups or species Escherichia coli, Enterobacter spp., Arthrobacter spp., Alcaligenes spp., Pseudomonas spp., and Corynebacterium glutamicum. The 257 isolates were screened for in-1. Eighty-one of the gram-negative isolates were also screened for the Tc-r genes tet(A), tet(B), and tet(C), and all (n = 72) gram-positive isolates were screened for tet(33). Fourteen (7%) of the soil isolates and eleven (25%) of the pigsty isolates contained in-1. All isolates that contained tet genes also contained ...
Introduction: Achromobacter xylosoxidans is a water‐borne organism that causes healthcare‐associated infections and has been isolated from blood, cerebrospinal fluid, stool, urine, sputum, peritoneal fluid, skin, ear discharge, wounds, abscesses, bone, joints, endocardium and central venous catheters, mostly in immunocompromised patients. Case presentation: We describe here the rare case of a young immunocompetent alcoholic male admitted with symptoms of acute pancreatitis who failed to improve with conventional management. Blood culture later showed the growth of A. xylosoxidans. The patient improved when he was treated with antibiotics as per the sensitivity report. Conclusion: Although Achromobacter is rarely isolated from clinical samples, it should never be assumed to be a contaminant as this infection has propensity for progression to fatal bacteraemia, even in apparently healthy individuals.
Achromobacter spp. are recognized as emerging pathogens in hospitalized as well as in cystic fibrosis (CF) patients. From 2012 to 2015, we collected 69 clinical isolates (41 patient) of Achromobacter spp. from 13 patients with CF (CF isolates, n = 32) and 28 patients receiving care for other health conditions (non-CF isolates, n = 37). Molecular epidemiology and virulence potential of isolates were examined. Antimicrobial susceptibility, motility, ability to form biofilms and binding affinity to mucin, collagen, and fibronectin were tested to assess their virulence traits. The nrdA gene sequencing showed that A. xylosoxidans was the most prevalent species in both CF and non-CF patients. CF patients were also colonized with A. dolens/A, ruhlandii, A. insuavis, and A. spiritinus strains while non-CF group was somewhat less heterogenous, although A. insuavis, A. insolitus, and A. piechaudii strains were detected beside A. xylosoxidans. Three strains displayed clonal distribution, one amon...g ...
To the Editor: The carbapenems (meropenem and imipenem), the ß-lactams with the broadest spectrum, are stable to most ß-lactamases (1). Therefore, they are often used as antibiotics of last resort for treating nosocomial infections due to gram-negative bacteria resistant to other ß-lactams. Resistance to carbapenems and susceptibility to other ß-lactams in Pseudomonas aeruginosa is common as a result of reduced drug accumulation or increased expression of pump efflux (1).. Several extended-spectrum ß-lactamases have been reported in P. aeruginosa, but only two, IMP-1 and VIM-1, possess an extended hydrolysis profile that includes carbapenems (2-5). The chromosome-borne and plasmid-mediated carbapenem-hydrolyzing ß-lactamase, IMP-1, has been described in several gram-negative rods, including P. aeruginosa, P. cepacia, Alcaligenes xylosoxydans, and Enterobacteriaceae isolates in Japan (4,6). Recently, a chromosome-borne carbapenem-hydrolyzing ß-lactamase, VIM-1, was reported from a clinical ...
1.Chen GQ, Wu Q. 2005. The application of polyhydroxyalkanoates as tissue engineering materials. Biomaterials. 26:6565-6578 2. Doi Y, Kunioka M, Nakamura Y, Soga K. 1986. Nuclear magnetic resonance studies on poly(B-hydroxybutyrate) and a copolyester of B-hydroxybutyrate and B-hydroxyvalerate isolated from Alcaligenes eutrophus H16. Macromolecules. 19:2860-2864 3. Endy D. 2005. Foundations for engineering biology. Nature. 438(7067):449-53 4. International Genetically Engineered Machines competition. 15 Jun 2008. 26 Jul 2008. ,http://igem.org, 5. Holmes PA. 1985. Applications of PHB - a microbially produced biodegradable thermoplastic. Physics in technology. 16:32-36 6. Kang Z, Wang Q, and H Zhang. 2008. Construction of a stress-induced system in Escherichia coli for efficient polyhydroxyalkanoates production. Biotechnological Products and Process Engineering. 79:203-208 7. Knight TF. 2003. Idempotent Vector Design for Standard Assembly of BioBricks. Tech. rep., MIT Synthetic Biology Working ...
Bacteria capable of degrading polymeric products were isolated from several sources including tap water, sediment, and a deteriorated polymeric product. Alcaligenes xylosoxidans; T2, Pseudomonas aeruginosa GP10, and Nocardia corynebacterioides S3 were able to utilize rubber products as a sole source of carbon and energy. These microorganisms showed different growth patterns in mineral salt media (MSM) supplemented with rubber strips or with the rubber extract. A. xylosoxidans T2, P. aeruginosa GP10 and N. corynebacterioides S3 reduced the weight of the rubber product by approximately 2.0, 4.0 and 5.3%, respectively, after 70 days of incubation with the rubber product in MSM. On average, 0.45 mg (water-soluble carbon) g(-1) of the rubber product was released into solution phase after 7 days of incubation. Growth of N. corynebacterloides S3 was initially slower but exceeded the other two bacteria upon colonization of the polymer products. N. corynebacterioides S3 formed a dense biofilm on surfaces ...
Design of Enzyme Reactor. Geoff Greer Joseph Gvildys Aaron McGregor April 2 nd , 2007. Enzyme Selection. Transpeptidation Trypsin or Achromobacter lyticus proteinase I Optimal enzyme conditions 4 o C and a pH of 7. Eli Lilly. Enzyme Immobilization. Benefits Slideshow 209542 by...
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Biopolymers for Geotechnical Applications. A number of bacteria, e.g., Alcaligenes eutrophus, have been employed to produce large amounts of intracellular polyester, poly-β-hydroxy butyrate or valerate. These polymer mixtures or copolymers are only sparingly soluble in water, and are stable between pH 3-11 under 10-100°C. These polymers are found to be ideal binders for soil that the strength of soil can be enhanced. For porous media such as sand or clay the permeability can be reduced to almost one million fold; therefore, the bacteria derived polymers are perfect plugging agents for stopping the plumes of subsurface pollutants. This is obviously usethl for on-site contaminant-control for hazardous wastes or chemicals. The microbial polymers, including the extracellulosic polymer, can be used as selective plugging agents, so that the concentration of pollutant can be partitioned (or bio-barrier) in different zones and pockets to allow bioremediation to follow (zonal bioremediation
ID PHG153 preliminary; circular DNA; SYN; 4381 BP. XX AC IG9825; XX DT 01-JUL-1995 (Rel. 11, Created) DT 01-JUL-1995 (Rel. 12, Last updated, Version 1) XX DE E. coli plasmid vector pHG153 - complete. XX KW cloning vector. XX OS Cloning vector OC Artificial sequences; Cloning vehicles. XX RN [1] RC pHG134 from pBR322 & linker RC pHG138 from pUC8 & linker RC pHG140 from pUC8 & linker RC pHG144 from pBR322 & linker RC pHG146 from pUC8 & linkers RC pHG153 from pBR322 & linkers RC pHG155 from pHG153 RC pHG161 from pBR322 & linker RC pHG163 from pHG155 RC pHG165 from pHG163 & pHG146 RC pHG171 from pHG165 & pUC9 RC pHG173 from pHG165 & linker RC pHG175 from pBR322 & linkers RC pHG177 from pHG175 RC pHG179 from pHG165 & pHG175 RC pHG327 from pHG165 & pUC18 RC pHG329 from pHG165 & pUC19 RC pHG331 from pHG146 & pUC18 RC pHG333 from pHG146 & pUC19 RC pHG335 from pHG146 & pUC9 RA Stewart G.S., Lubinsky-Mink S., Jackson C.G., Cassel A., Kuhn J.; RT pHG165: a pBR322 copy number derivative of pUC8 for cloning ...
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Looking for online definition of Achromobacter xylosoxidans in the Medical Dictionary? Achromobacter xylosoxidans explanation free. What is Achromobacter xylosoxidans? Meaning of Achromobacter xylosoxidans medical term. What does Achromobacter xylosoxidans mean?
Biological degradation of phenol by Alcaligenes faecalis with high biodegradation activity and high tolerance was investigated at 25C. Phenol could be utilized by the bacteria as the sole carbon and energy sources. The cell growth and substrate degradation of phenol as single substrates for Alcaligenes faecalis in batch cultures (shaking flasks) were investigated at different initial phenol concentrations. Phenol was observed to be an inhibitory compound. Particularly, when free cells grew on a high concentration of phenol, substrate inhibition was observed and the higher the concentration of phenol, the longer was the lag period. The lag time and whole required time for phenol biodegradation was considerably decreased by immobilized cells due to improvement of cells resistance against high Phenol concentration by cell immobilization compared to free cells. Required time for completely degrade initial phenol of 700 and 1000 ppm by free and immobilized cells is 56, 101 and 35, 72 hours ...
In Pseudomonas aeruginosa, quorum sensing (QS) regulates the production of secondary metabolites, many of which are antimicrobials that impact on polymicrobial community composition. Consequently, quenching QS modulates the environmental impact of P. aeruginosa. To identify bacteria capable of inactivating the QS signal molecule 2-heptyl-3- hydroxy-4(1H)-quinolone (PQS), a minimal medium containing PQS as the sole carbon source was used to enrich a Malaysian rainforest soil sample. This yielded an Achromobacter xylosoxidans strain (Q19) that inactivated PQS, yielding a new fluorescent compound (I-PQS) confirmed as PQS-derived using deuterated PQS. The I-PQS structure was elucidated using mass spectrometry and nuclear magnetic resonance spectroscopy as 2-heptyl-2-hydroxy-1,2-dihydroquinoline- 3,4-dione (HHQD). Achromobacter xylosoxidans Q19 oxidized PQS congeners with alkyl chains ranging from C1 to C5 and also N-methyl PQS, yielding the corresponding 2-hydroxy-1,2-dihydroquinoline-3,4- diones, ...
TY - JOUR. T1 - NiFe hydrogenase active site biosynthesis. T2 - Identification of Hyp protein complexes in Ralstonia eutropha. AU - Jones, Anne K.. AU - Lenz, Oliver. AU - Strack, Angelika. AU - Buhrke, Thorsten. AU - Friedrich, Bärbel. PY - 2004/10/26. Y1 - 2004/10/26. N2 - Biosynthesis of the NiFe hydrogenase active site is a complex process involving the action of the Hyp proteins: HypA-HypF. Here we investigate the mechanism of NiFe site biosynthesis in Ralstonia eutropha by examining the interactions between HypC, HypD, HypE, and HypF1. Using an affinity purification procedure based on the Strep-tag, II, we purified HypC and HypE from different genetic backgrounds as complexes with other hydrogenase-related proteins and characterized them using immunological analysis. Copurification of HypC and HoxH, the active site-containing subunit of the soluble hydrogenase in R. eutropha, from several different genetic backgrounds suggests that this complex forms early in the maturation process. With ...
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This study aimed at producing C6-C8 medium-chain carboxylates (MCCAs) directly from gaseous CO using mixed culture. The yield and C2-C8 product composition were investigated when CO was continuously fed with gradually increasing partial pressure. The maximal concentrations of n-caproate, n-heptylate, and n-caprylate were 1.892, 1.635, and 1.033 mmol L−1, which were achieved at the maximal production rates of 0.276, 0.442, and 0.112 mmol L−1 day−1, respectively. Microbial analysis revealed that long-term acclimation and high CO partial pressure were important to establish a CO-tolerant and CO-utilizing chain-elongating microbiome, rich in Acinetobacter, Alcaligenes, and Rhodobacteraceae and capable of forming MCCAs solely from CO. These results demonstrated that carboxylate and syngas platform could be integrated in a shared growth vessel, and could be a promising one-step technique to convert gaseous syngas to preferable liquid biochemicals, thereby avoiding the necessity to coordinate syngas
Methyl 2-bromo-5-fluorobenzoate; CAS Number: 6942-39-8; find Apollo Scientific Ltd-APO455834847 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich
ID W8WYL2_CASDE Unreviewed; 561 AA. AC W8WYL2; DT 14-MAY-2014, integrated into UniProtKB/TrEMBL. DT 14-MAY-2014, sequence version 1. DT 25-OCT-2017, entry version 19. DE SubName: Full=Glycerol-3-phosphate dehydrogenase {ECO:0000313,EMBL:CDM24848.1}; DE EC=1.1.5.3 {ECO:0000313,EMBL:CDM24848.1}; GN ORFNames=BN940_11951 {ECO:0000313,EMBL:CDM24848.1}; OS Castellaniella defragrans 65Phen. OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; OC Alcaligenaceae; Castellaniella. OX NCBI_TaxID=1437824 {ECO:0000313,EMBL:CDM24848.1, ECO:0000313,Proteomes:UP000019805}; RN [1] {ECO:0000313,EMBL:CDM24848.1, ECO:0000313,Proteomes:UP000019805} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=65Phen {ECO:0000313,EMBL:CDM24848.1}; RX PubMed=24952578; DOI=10.1186/1471-2180-14-164; RA Petasch J., Disch E.M., Markert S., Becher D., Schweder T., Huttel B., RA Reinhardt R., Harder J.; RT The oxygen-independent metabolism of cyclic monoterpenes in RT Castellaniella defragrans 65Phen.; RL BMC ...
Research outputs, collaborations and relationships for Research Center for Zoonosis Control (CZC), Hokkaido University published between 1 December 2019 - 30 November 2020 as tracked by the Nature Index.
Black dots contaminant phenonmenon: helpful paper - posted in Tissue and Cell Culture: Dear all,It seems like many researcher faced with this bizarre black dancing dots in cell culture, and me too! So Ive search about this issue and found one paper that published in Biologicals 2010. Gray JS, Birmingham JM, Fenton JI.Got black swimming dots in your cell culture? Identification of Achromobacter as a novel cell culture contaminant Biologicals. 2010 Mar;38(2):273-7. Epub 2009 Nov 18....
Acorda Therapeutics Inc (NASDAQ: ACOR), a biopharmaceutical company that develops therapies for neurological disorders, on Tuesday announced that ...
This webcast is for JMP Clinical users. See examples of how to use JMP Clinical to examine adverse events, abnormal findings and drug efficacy indicators to help expedite the review, analysis and reporting of clinical trials.
Di*late .] [L. dilatare; either fr. di- = dis- + latus wide, not the same word as latus, used as p. p. of ferre to bear (see Latit...
The name Alcaligenes has its origin in Arabic and Greek and means "alkali-producing". It was named in 1919. "Alcaligenes - ... "Alcaligenes". "Alcaligenes". Malek-Marín T et al. (2009) A case of endocarditis of difficult diagnosis in dialysis: could "pest ... Alcaligenes is a genus of Gram-negative, aerobic, rod-shaped bacteria. The species are motile with amphitrichous flagella and ... Strains of Alcaligenes (such as A. faecalis) are found mostly in the intestinal tracts of vertebrates, decaying materials, ...
Type strain of Alcaligenes aquatilis at BacDive - the Bacterial Diversity Metadatabase v t e (All articles with dead external ... Alcaligenes aaquatilis is a Gram-negative, catalase- and cytochrome oxidase-positive, motile bacterium with peritrichous ... Straininfo of Alcaligenes aquatilis International Journal of Systematic and Evolutionary Microbiology [1][permanent dead link] ... "Genomic and Physiological Traits of the Marine Bacterium Alcaligenes aquatilis QD168 Isolated From Quintero Bay, Central Chile ...
... is a bacterium which can produce ropiness in milk and which can grow in sun tea. [1] "Alcaligenes ...
... is a Gram-negative aerobic bacterium used for bioremediation purposes of oil pollution, pesticide ... Based on 16S rRNA analysis, P. alcaligenes has been placed in the P. aeruginosa group. O'Mahony, MM; Dobson, AD; Barnes, JD; ... Type strain of Pseudomonas alcaligenes at BacDive - the Bacterial Diversity Metadatabase v t e (Articles with short description ... Valenstein, P; Bardy, GH; Cox, CC; Zwadyk, P (1983). "Pseudomonas alcaligenes endocarditis". American Journal of Clinical ...
... is a bacterium; its type strain is CIP 60.75 (= Hugh 366-5 = IAM 12591 = LMG 1873). It is rod-shaped, ... Kiredjian, M.; Holmes, B.; Kersters, K.; Guilvout, I.; De Ley, J. (1986). "Alcaligenes piechaudii, a New Species from Human ... ISBN 0-387-25495-1. Genus Alcaligenes. LPSN, bacterio.net v t e (Articles with short description, Short description matches ... Peel MM, Hibberd AJ, King BM, Williamson HG (August 1988). "Alcaligenes piechaudii from chronic ear discharge". Journal of ...
... is a bacterium from the genus Alcaligenes. IPSN lpsn.dsmz.de Straininfo of Alcaligenes aestus Taxonomy ...
... motile bacterium from the genus Alcaligenes. IPSN lpsn.dsmz.de Straininfo of Alcaligenes defragrans Taxonomy Browser ... Alcaligenes defragrans is a Gram-negative, oxidase- and catalase-positive, strictly aerobic, ...
"SPECIES Alcaligenes faecalis". UniProt. Georg M. Garrity. Sc.D. (2005). "The Proteobacteria Part C The Alpha-, Beta-, Delta-, ... Alcaligenes denitrificans is a Gram-negative, oxidase- and catalase-positive, strictly aerobic, motile bacterium with ... denitrificans). "Genus Alcaligenes". LPSN. List of prokaryotic names with standing in nomenclature (LSPN). Retrieved 2013-07-20 ... "Alcaligenes faecalis". Straininfo.net. Archived from the original on August 26, 2014. "Achromobacter denitrificans". DSMZ ...
... is a species of Gram-negative, rod-shaped bacteria commonly found in the environment. It was originally ... Type strain of Alcaligenes faecalis at BacDive - the Bacterial Diversity Metadatabase v t e (Articles with short description, ... In 2001, previously unidentified isolates of Alcaligenes were classified as a new subspecies of A. faecalis: A. faecalis ... Mitchell, R. G.; Clarke, S. K. R. (1965). "An Alcaligenes Species with Distinctive Properties Isolated from Human Sources". ...
... is a bacterium which has been reclassified to Deleya pacifica. IPSN lpsn.dsmz.de Straininfo of ... Alcaligenes pacificus Taxonomy Browser The Taxonomicon Bergey´s Manual of Determinative Bacteriology ninth Edition John G. Holt ...
... is a bacterium from the genus Alcaligenes which was isolated from seawater. IPSN lpsn.dsmz.de Straininfo of ... Alcaligenes cupidus Taxonomy Browser WoRMS World Register of Marine Species [1] ATCC Taxonomy Browser[permanent dead link] v t ...
Alcaligenes sp., Acinetobacter lwoffi, Flavobacterium sp., Micrococcus roseus and Corynebacterium sp, isolated from the ...
Bacteria which give negative results for the indole test include: Actinobacillus spp., Aeromonas salmonicida, Alcaligenes sp., ...
stewartii) succinoglycan (Alcaligenes faecalis var. myxogenes, Sinorhizobium meliloti) xanthan (Xanthomonas campestris) welan ( ... Alcaligenes spp.) Application of Nanoparticles has shed new light on how to target biofilms due to their high surface-area-to- ... Alcaligenes faecalis var. myxogenes) cyclosophorans (Agrobacterium spp., Rhizobium spp. and Xanthomonas spp.) dextran ( ... Alcaligenes viscosus, Zymomonas mobilis, Bacillus subtilis) pullulan (Aureobasidium pullulans) scleroglucan (Sclerotium rolfsii ...
Krebs, H. A.; Whittam, R.; Hems, R. (May 1957). "Potassium uptake of Alcaligenes faecalis". Biochemical Journal. 66 (1): 55-60 ...
... blue copper protein from Alcaligenes faecalis; cupredoxin (CPC) from Cucumis sativus (Cucumber) peelings; cusacyanin (basic ...
Schneider, K.; Cammack, R.; Schlegel, G. & Hall, D. (1979). "The iron-sulphur centres of soluble hydrogenase from Alcaligenes ... H. eutrophus was then renamed Alcaligenes eutropha because it was a micro-organism with degenerated peritrichous flagellation. ... 1995). "Transfer of two Burkholderia and an Alcaligenes species to Ralstonia gen. nov.: proposal of Ralstonia pickettii ( ... Schneider, K.; Schlegel, H. (1976). "Purification and properties of soluble hydrogenase from Alcaligenes eutrophus H 16". ...
Deveryshetty J, Phale PS (October 2010). "Biodegradation of phenanthrene by Alcaligenes sp. strain PPH: partial purification ...
for Alcaligenes paradoxus (Davis 1969). 1991. International Journal of Systematic Bacteriology. 41: 445-450 PDF Online[ ...
... were originally classified in the genera Alcaligenes along with V. paradoxus (Alcaligenes eutrophus and Alicaligenes paradoxus ... nov., for Alcaligenes paradoxus (Davis 1969)". International Journal of Systematic Bacteriology. 41 (3): 445-450. doi:10.1099/ ...
It can be found in Alcaligenes species. The enzyme 4-chlorobenzoate dehalogenase uses 4-chlorobenzoate and H2O to produce 4- ...
... pp.294-300 Received as revised 22 February The Lysis of Gram-negative Alcaligenes eutrophus and Alcaligenes latus by Palmitoyl ... Straininfo of Alcaligenes latus UniProt Lee, SY; Choi Ji, Han K; Song, JY (1999). "Removal of endotoxin during purification of ... Alcaligenes latus has been reclassified as Azohydromonas lata. "IPSN LPSN". Retrieved 14 October 2017. ... "Reclassification of Alcaligenes latus strains IAM 12599T and IAM 12664 and Pseudomonas saccharophila as Azohydromonas lata gen ...
Wakayama M, Hayashi S, Yatsuda Y, Katsuno Y, Sakai K, Moriguchi M (1996). "Overproduction of D-aminoacylase from Alcaligenes ... "Cloning and sequencing of a gene encoding D-aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6 and expression ...
Nakajima M, Shirokane Y, Mizusawa K (1980). "A new amidinohydrolase, methylguanidine amidinohydrolase from Alcaligenes sp. N-42 ...
Ermler U, Siddiqui RA, Cramm R, Friedrich B (December 1995). "Crystal structure of the flavohemoglobin from Alcaligenes ... "Phospholipid bound to the flavohemoprotein from Alcaligenes eutrophus". Eur. J. Biochem. 262 (2): 396-405. doi:10.1046/j.1432- ...
Open pan-genome has been observed in environmental isolates such as Alcaligenes sp. and Serratia sp., showing a sympatric ... Basharat Z, Yasmin A, He T, Tong Y (2018). "Genome sequencing and analysis of Alcaligenes faecalis subsp. phenolicus MB207". ...
Hiatt, Howard H; Horecker, B L (13 October 1955). "D-erythrose metabolism in a strain of Alcaligenes faecalis". Journal of ...
In Alcaligenes eutrophus the gene for this is hoxN. Well known nickel organometalic (or organonickel) compounds include ...
It was reported to be produced by Alcaligenes faecalis var. myxogenes. Subsequently, the taxonomy of this non-pathogenic ... Production by a Mutant of Alcaligenes faecalis var myxogenes in Defined Medium". Agric Biol Chem. 30: 764-769. doi:10.1271/ ...
Mauger J, Nagasawa T, Yamada H (1990). "Occurrence of a novel nitrilase, arylacetonitrilase, in Alcaligenes faecalis JM3". Arch ... Nagasawa T, Mauger J, Yamada H (1990). "A novel nitrilase, arylacetonitrilase, of Alcaligenes faecalis JM3 Purification and ...
Department of Chemistry, UBC Faculty of Science. Vancouver Campus. 2036 Main Mall. Vancouver, BC Canada V6T 1Z1. Tel: 604.822.3266. Fax: 604.822.2847. ...
Is Alcaligenes faecalis VP positive?. Alcaligenes faecalis, used for an earlier test, is oxidase positive and Escherichia coli ... What is the shape of Alcaligenes faecalis?. Alcaligenes faecalis is a species of gram-negative, rod-shaped, aerobic bacteria ... What Gram stain is Alcaligenes faecalis?. Alcaligenes faecalis (A. faecalis) is a Gram-negative, obligate aerobic, oxidase- ... Is the Alcaligenes faecalis positive or negative?. A. faecalis is a Gram-negative bacterium which appears rod-shaped and motile ...
The growth behaviour of Alcaligenes eutrophus using various concentrations of benzoate was investigated. In batch culture, ... Pieper D. H., Engesser K.-H, Don R. H., Timmis K. N., Knackmuss H.-J. 1985; Modified or/Áo-cleavage pathway in Alcaligenes ... Hughes E.J, L. and Bayly R. C. 1983; Control of the meta- cleavage pathway of Alcaligenes eutrophus. J Bacteriol 154:1363-1370 ... Johnson B.F., Stanier R. Y. 1971b; Regulation of the ß- ketoadipate pathway in Alcaligenes eutrophus. J Bacteriol 107:476-485 ...
Arabic meanings of word Alcaligenes . English to Arabic dictionary gives you the best and accurate Arabic meanings of ... Alcaligenes meaning in Arabic has been searched times till 06 Dec, 2022. The definitions of the word Alcaligenes has been ... تعريفات الكلمة Alcaligenes تم وصفه هنا بأقصى قدر من التفاصيل، وأيضا تغريم المرادفات المختلفة للكلمة Alcaligenes ۔ يمكنك العثور ... Alcaligenes ترجمة باللغة العربية: يمكن أن يكون البحث عن المعاني باللغة العربية مفيدًا لفهم السياق بطريقة فعالة۔ يمكنك الحصول ...
Desai N, Gupta SK, Menon RK, Seth V. Alcaligenes fecalis septicemia. Indian Journal of Pediatrics. 1983 Nov-Dec; 50(407): 687-9 ...
keywords = "Alcaligenes aquatilis strain YGD 2906, Biosurfactant production, HPLC-MS/MS, MALDI-TOF/MS analysis, Petroleum ... The petroleum-degrading bacteria Alcaligenes aquatilis strain YGD 2906 as a potential source of lipopeptide biosurfactant. Fuel ... The petroleum-degrading bacteria Alcaligenes aquatilis strain YGD 2906 as a potential source of lipopeptide biosurfactant. In: ... Dive into the research topics of The petroleum-degrading bacteria Alcaligenes aquatilis strain YGD 2906 as a potential source ...
P. alcaligenes CCUG 1868 P. alcaligenes CCUG 5004 P. alcaligenes CCUG 6697 B P. alcaligenes CCUG 7819 P. alcaligenes CCUG 32134 ... P. alcaligenes 1577, DSM 50342, ATCC 14909, NCTC ... (type strain) P. alcaligenes OT, DSM 19550 P. alcaligenes CIP 104975, CCUG ... P. alcaligenes CIP 104976, CCUG 13700, CCUG 13700 ... P. alcaligenes CCUG 226, NCPPB 1959, ATCC 17665, ... ... Pseudomonas alcaligenes CIP 104974 is a mesophilic bacterium of the family Pseudomonadaceae. ...
Crystal Structure of H313Q Mutant of Alcaligenes Xylosoxidans Nitrite Reductase with Nitrite Bound ... The structure of Crystal Structure of H313Q Mutant of Alcaligenes Xylosoxidans Nitrite Reductase with Nitrite Bound also ... Zinc in PDB 1wa2: Crystal Structure of H313Q Mutant of Alcaligenes Xylosoxidans Nitrite Reductase with Nitrite Bound. Protein ... The structure of Crystal Structure of H313Q Mutant of Alcaligenes Xylosoxidans Nitrite Reductase with Nitrite Bound, PDB code: ...
STRUCTURE OF ALCALIGENES FAECALIS NITRITE REDUCTASE REDUCED WITH ASCORBATE ... STRUCTURE OF ALCALIGENES FAECALIS NITRITE REDUCTASE REDUCED WITH ASCORBATE Coordinates. PDB Format Method. X-RAY DIFFRACTION ... Murphy, M.E. et al., Structure of nitrite bound to copper-containing nitrite reductase from Alcaligenes faecalis. Mechanistic ...
Alcaligenes xylosoxidans, A. faecalis, Aeromonas hydrophila, Aeromonas spp. Elizabethkingia anaophelis, E. meningosepticum ...
Heterologous overproduction of the copper proteins nitrite reductase and azurin I from Alcaligenes xylosoxidans in Escherichia ... conditions for the overproduction of functional copper-containing nitrite reductase and azurin from Alcaligenes xylosoxidans. ...
ALCALIGENES POLYSACCHARIDES , GLUCOSE , CITRIC ACID , [+ / - (MAY CONTAIN) , CI 77007 (ULTRAMARINES) , CI 77491, , CI 77492, , ...
Alcaligenes venustus Baumann et al. Depositors. P Baumann Type of isolate. Environmental Cross references. GenBank L42618 16S ... created to accommodate the marine species Alcaligenes aestus, A. pacificus, A. cupidus, A. venustus, and Pseudomonas marina. ...
Categories: Alcaligenes Image Types: Photo, Illustrations, Video, Color, Black&White, PublicDomain, CopyrightRestricted 10 ...
Gentamicin is active against the following organisms isolated from canine skin: Alcaligenes sp., Citrobacter sp., Klebsiella sp ...
Alcaligenes xylosoxydans. Fusarium sp. Rhizoctonia bataticola. (Swiontek Brzezinska et al., 2014). Stenotrohomonas maltophila ...
Alcaligenes]],br> [[Alcanivorax]],br> [[Aliivibrio fischeri]],br> [[Anabaena]],br> [[Aquifex]],br> [[ ...
Alcaligenes faecalis Cellulitis After a Dog Bite: Case Report and Literature Review. Chu AS, Harkness J. Chu AS, et al. Pediatr ...
d1qlwa_ c.69.1.15 (A:) A novel bacterial esterase {Alcaligenes sp.} ...
Agerso, Y., and Sandvang, D. (2005). Class 1 integrons and tetracycline resistance genes in Alcaligenes, Arthrobacter, and ...
Fatima, T.; Mishra, I.; Verma, R.; Arora, N.K. Mechanisms of halotolerant plant growth promoting Alcaligenes sp. involved in ...
Alcaligenes faecalis. Alphavirus. Anaplasma marginale. Aphtovirus. Bacillus anthracis. Bacteroides nodosus. Bordetella ...
Alcaligenes xylosoxidans Acremonium spp Typical time frame of postoperative endophthalmitis. Staphylococcus epidermidis within ...
2] "The czc operon of Alcaligenes eutrophus CH34: from resistance mechanism to the removal of heavy metals." Diels L.et.al. ... 1] "Expression and nucleotide sequence of a plasmid-determined divalent cation efflux system from Alcaligenes eutrophus." Nies ...
  • Pseudomonas alcaligenes CIP 104974 is a mesophilic bacterium of the family Pseudomonadaceae. (dsmz.de)
  • Aspergillus fumigatus predominated among the fungi, whereas Bacillus and gram-negative organisms (Pseudomonas, Alcaligenes, Citrobacter, and Klebsiella species) prevailed among bacteria. (cdc.gov)
  • Denitrifiers in forests include Alcaligenes, Pseudomonas and Bacillus species as well as Actinobacteria (especially Streptomycetes). (ottovonschirach.com)
  • 1 Gentamicin is active against Pseudomonas aeruginosa , indole-positive and -negative Proteus species, Escherichia coli , Klebsiella species, Enterobacter species, Alcaligenes species, Staphylococcus species, and Streptococcus species. (nih.gov)
  • Especie de Pseudomonas, no patógena, que se encuentra en la tierra, especialmente en la RIZOSFERA, alrededor de las raíces de las plantas, así como en la superficie de las hojas. (bvsalud.org)
  • Some During studies of ear disharge caused by otitis media, patients in the geographic area also used cockroach paste, we detected the gram-negative bacillus Alcaligenes faeca- palm oil, sweet olive oil, sewing machine oil, or breast lis in addition to the commonly isolated bacterial species milk to prevent ear discharge. (cdc.gov)
  • Their samples also were more likely to contain bacteria of the genus Alcaligenes, whose role in CF is not yet known. (rtmagazine.com)
  • other species, including founded by the difficulty in accurate species identification by Stenotrophomonas maltophilia , Alcaligenes ( Achromobacter ) using standard microbiologic techniques. (cdc.gov)
  • The type and reference strains of Ralstonia , Pandoraea , Burkholderia , Alcaligenes , and Bordetella species have been described (9-14). (cdc.gov)
  • Heterologous overproduction of the copper proteins nitrite reductase and azurin I from Alcaligenes xylosoxidans in Escherichia coli is described. (iucr.org)
  • The growth behaviour of Alcaligenes eutrophus using various concentrations of benzoate was investigated. (microbiologyresearch.org)
  • Evidence for novel mechanisms of polychlorinated biphenyl metabolism by Alcaligenes eutrophus H850. (microbiologyresearch.org)
  • Dois M. , Ampe F. , Lindley N. D. 1994 Effects of oxygen limitations on benzoate degradation by Alcaligenes eutrophus IAWQ 17th Biennial Conference, July 94, Budapest, Hungary. (microbiologyresearch.org)
  • Transposon mutagenesis and cloning analysis of the pathway for the degradation of 2,4-dichlorophenoxyacetic acid and 3-chlorobenzoate in Alcaligenes eutrophus JMP134(pJP4). (microbiologyresearch.org)
  • 1] "Expression and nucleotide sequence of a plasmid-determined divalent cation efflux system from Alcaligenes eutrophus. (tcdb.org)
  • 2] "The czc operon of Alcaligenes eutrophus CH34: from resistance mechanism to the removal of heavy metals. (tcdb.org)
  • Insights Into Redox Partner Interactions and Substrate Binding in Nitrite Reductase From Alcaligenes Xylosoxidans: Crystal Structures of the TRP138HIS and HIS313GLN Mutants Biochemistry V. 43 16311 2004 . (atomistry.com)
  • The strain was identified as Alcaligenes aquatilis YGD 2906 using phenotypic characterization and 16S ribosomal RNA sequencing. (ulster.ac.uk)
  • You can find words like Alcaligenes from Hamariweb.com dictionary in multiple languages like Urdu, Hindi, Spanish, French and other languages. (hamariweb.com)
  • You have searched the English word "Alcaligenes" which meaning "" in Arabic. (hamariweb.com)
  • Use of random amplified polymorphic DNA PCR to examine epidemiology of Stenotrophomonas maltophilia and Achromobacter (Alcaligenes) xylosoxidans from patients with cystic fibrosis. (nih.gov)
  • CAPD-associated peritonitis caused by Alcaligenes xylosoxidans sp. (hku.hk)
  • However, certain bacteria such as Aeromonas hydrophila and Alcaligenes xylosoxidans are known to cause diseases in pigs if they get into their respiratory tract. (dataintelo.com)
  • Structure of nitrite bound to copper-containing nitrite reductase from Alcaligenes faecalis. (expasy.org)
  • Directing the mode of nitrite binding to a copper-containing nitrite reductase from Alcaligenes faecalis S-6: Characterization of an active site isoleucine. (expasy.org)
  • A combined fluorescence and electrochemical method is described that is used to simultaneously monitor the type-1 copper oxidation state and the nitrite turnover rate of a nitrite reductase (NiR) from Alcaligenes faecalis S-6. (sigmaaldrich.com)
  • Proteus mirabilis was the second roquinolone-resistant Alcaligenes faecalis , commonly found most common bacterium (n = 7), followed by Klebsiella in birds. (cdc.gov)
  • Fragments of chromosomal DNA from Alcaligenes latus DSM1124 were cloned into Escherichia coli and transformants were screened for poly(D(-)-3-hydroxybutyrate) [P(3HB)] production during excess carbon supply. (nih.gov)
  • Production of polyhydroxybutyrate (PHB) by Alcaligenes latus using sugarbeet juice. (ncsu.edu)
  • Upstream process optimization of polyhydroxybutyrate (PHB) by Alcaligenes latus using two-stage batch and fed-batch fermentation strategies. (ncsu.edu)
  • Wang, B. Q., Sharma-Shivappa, R. R., Olson, J. W., & Khan, S. A. Upstream process optimization of polyhydroxybutyrate (PHB) by Alcaligenes latus using two-stage batch and fed-batch fermentation strategies. (ncsu.edu)
  • Klebsiella pneumonia strain B21 (gi: 922317936), Klebsella pneumonia strain ICB-C183 (gi: 908478837), Klebsella oxytoca strain BCNAI (gi: 846993354), Klebsella oxytoca strain BC4 (gi: 884060138), Alcaligenes faecalis strain IOU PMR (gi: 686028963) and Alcaligenes faecalis strain AQ-I (gi: 816845513). (ajol.info)
  • faecalis alcaligenes v etiologii mochevykh kamnei. (nih.gov)
  • 1999. Classification of Alcaligenes faecalis-like isolates from the environment and human clinical sam¬ples as Ralstonia gilardii sp. (exeley.com)
  • Pathway of oxidation of pyruvic oxime by a heterotrophic nitrifier of the genus Alcaligenes: evidence against nitroethane as an intermediate. (semanticscholar.org)
  • The oxygenase component of the 2-aminobenzenesulfonate dioxygenase system from Alcaligenes sp. (genome.jp)
  • Extension of resolution and oligomerization‐state studies of 2,4′‐dihydroxyacetophenone dioxygenase from Alcaligenes sp. (infona.pl)
  • 4.1.1.76, originated from Alcaligenes bronchisepticus KU 1201) is an enzyme which catalyzes asymmetric decarboxylation of arylmalonate. (elsevier.com)
  • We found that 20 (10.6%) of 188 patients with chronic suppurative otitis media in Angola were co-colonized with fluoroquinolone-resistant Alcaligenes faecalis , commonly found in birds. (cdc.gov)
  • This reference will give you details (Crystal Structure of an Electron Transfer Complex between Aromatic Amine Dehydrogenase- Azurin from Alcaligenes faecalis, Biochemistry, 45, 13500-13510, 2006). (mail-archive.com)
  • Lipid A (Alcaligenes faecalis) purchased from PeptaNova is made by chemical synthesis and is not isolated from biological sources. (peptanova.de)
  • Género de bacterias gramnegativas aerobias y móviles que se encuentran en el agua y en el suelo. (bvsalud.org)
  • En ocasiones, estas bacterias causan infecciones oportunistas en humanos. (bvsalud.org)
  • Para cada lote de nauplios se hizo seguimiento de variables cualitativas (origen, modo de transporte, presencia de bacterias en el agua de transporte) y variables cuantitativas (tiempo y densidad de transporte, características de la calidad del agua, proporciones de cada estadío y deformidad de los nauplios). (aquadocs.org)
  • 0.05) sobre la sobrevivencia en larvicultura fueron el modo de transporte (en avioneta con sobrevivencia promedio de 45%, contra 35% en aeronave comercial presurizada), la presencia de bacterias (crecimiento en medio T.C.B.S.) en el agua de transporte de los nauplios (sobrevivencia sin dichas bacterias del 59% contra 40% con ellas) y el porcentaje de nauplios deformes. (aquadocs.org)
  • Estas bacterias se desarrollan en muchos medios de cultivo, tanto líquidos como sólidos de base agar, entre los que tenemos: Agar nutritivo, Agar Mac conkey, Agar Chocolate. (aramram.tv)
  • Electron donors such as glucose, methanol, and [https://en.wikipedia.org/wiki/Nicotinamide_adenine_dinucleotide NADH] are energy sources that can be thought of as "giving up" their electrons, while another molecule is in need to receive said electrons. (kenyon.edu)
  • The biochemical pathway and catabolic genes for chlorobenzoate degradation encoded on the transposon Tn5271 of Alcaligenes SP. (carleton.ca)
  • produced by the marine bacterium Alcaligenes sp. (mpg.de)
  • RÉSUMÉ Cette étude prospective a été menée dans six hôpitaux d'Edmonton (Canada) a n de déterminer les facteurs associés à l'obtention d'expectorations à des ns de mise en culture et les effets de celle-ci sur la prise en charge des patients atteints de pneumonie communautaire (PC). (who.int)