Alcaligenes: A genus of gram-negative, aerobic, motile bacteria that occur in water and soil. Some are common inhabitants of the intestinal tract of vertebrates. These bacteria occasionally cause opportunistic infections in humans.Alcaligenes faecalis: The type species of gram negative bacteria in the genus ALCALIGENES, found in soil. It is non-pathogenic, non-pigmented, and used for the production of amino acids.Pseudomonas alcaligenes: A species of gram-negative bacteria in the genus PSEUDOMONAS. It cannot utilize FRUCTOSE; GLUCOSE; or MALTOSE for energy.Chlorobenzoates: Benzoic acid or benzoic acid esters substituted with one or more chlorine atoms.Hydrogenase: An enzyme found in bacteria. It catalyzes the reduction of FERREDOXIN and other substances in the presence of molecular hydrogen and is involved in the electron transport of bacterial photosynthesis.Pseudomonas: A genus of gram-negative, aerobic, rod-shaped bacteria widely distributed in nature. Some species are pathogenic for humans, animals, and plants.Achromobacter: A genus of gram-negative, strictly aerobic, non-spore forming rods. Soil and water are regarded as the natural habitat. They are sometimes isolated from a hospital environment and humans.Biodegradation, Environmental: Elimination of ENVIRONMENTAL POLLUTANTS; PESTICIDES and other waste using living organisms, usually involving intervention of environmental or sanitation engineers.Biofeedback, Psychology: The therapy technique of providing the status of one's own AUTONOMIC NERVOUS SYSTEM function (e.g., skin temperature, heartbeats, brain waves) as visual or auditory feedback in order to self-control related conditions (e.g., hypertension, migraine headaches).Polyesters: Polymers of organic acids and alcohols, with ester linkages--usually polyethylene terephthalate; can be cured into hard plastic, films or tapes, or fibers which can be woven into fabrics, meshes or velours.Achromobacter denitrificans: The type species of gram negative, aerobic bacteria in the genus ACHROMOBACTER. Previously in the genus ALCALIGENES, the classification and nomenclature of this species has been frequently emended. The two subspecies, Achromobacter xylosoxidans subsp. denitrificans and Achromobacter xylosoxidans subsp. xylosoxidans are associated with infections.Nitrite Reductases: A group of enzymes that oxidize diverse nitrogenous substances to yield nitrite. (Enzyme Nomenclature, 1992) EC 1.Sulfanilic Acids: Aminobenzenesulfonic acids. Organic acids that are used in the manufacture of dyes and organic chemicals and as reagents.Genes, Bacterial: The functional hereditary units of BACTERIA.Acetoin: A product of fermentation. It is a component of the butanediol cycle in microorganisms. In mammals it is oxidized to carbon dioxide.Nickel: A trace element with the atomic symbol Ni, atomic number 28, and atomic weight 58.69. It is a cofactor of the enzyme UREASE.Hydroxybutyrates: Salts and esters of hydroxybutyric acid.Comamonas testosteroni: A species of gram-negative, aerobic rods formerly called Pseudomonas testosteroni. It is differentiated from other Comamonas species by its ability to assimilate testosterone and to utilize phenylacetate or maleate as carbon sources.Dioxygenases: Non-heme iron-containing enzymes that incorporate two atoms of OXYGEN into the substrate. They are important in biosynthesis of FLAVONOIDS; GIBBERELLINS; and HYOSCYAMINE; and for degradation of AROMATIC HYDROCARBONS.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Adipates: Derivatives of adipic acid. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain a 1,6-carboxy terminated aliphatic structure.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Catechols: A group of 1,2-benzenediols that contain the general formula R-C6H5O2.Cupriavidus necator: A gram-negative, facultatively chemoautotrophic bacterium, formerly called Wautersia eutropha, found in water and soil.Gram-Negative Bacterial Infections: Infections caused by bacteria that show up as pink (negative) when treated by the gram-staining method.Oxygenases: Oxidases that specifically introduce DIOXYGEN-derived oxygen atoms into a variety of organic molecules.Alcaligenaceae: A family of gram-negative, aerobic, non-spore forming rods or cocci. Well known genera include ACHROMOBACTER; ALCALIGENES; and BORDETELLA.Gentisates: Salts and esters of gentisic acid.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Conjunctival Neoplasms: Tumors or cancer of the CONJUNCTIVA.Oxidoreductases: The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)Plasmids: Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.Cross Protection: Protection conferred on a host by inoculation with one strain or component of a microorganism that prevents infection when later challenged with a similar strain. Most commonly the microorganism is a virus.Bacterial Proteins: Proteins found in any species of bacterium.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Hydrogen Bonding: A low-energy attractive force between hydrogen and another element. It plays a major role in determining the properties of water, proteins, and other compounds.Conjugation, Genetic: A parasexual process in BACTERIA; ALGAE; FUNGI; and ciliate EUKARYOTA for achieving exchange of chromosome material during fusion of two cells. In bacteria, this is a uni-directional transfer of genetic material; in protozoa it is a bi-directional exchange. In algae and fungi, it is a form of sexual reproduction, with the union of male and female gametes.

Analysis of the nitrous oxide reduction genes, nosZDFYL, of Achromobacter cycloclastes. (1/705)

The structural gene, nosZ, for the monomeric N2O reductase has been cloned and sequenced from the denitrifying bacterium Achromobacter cycloclastes. The nosZ gene encodes a protein of 642 amino acid residues and the deduced amino acid sequence showed homology to the previously derived sequences for the dimeric N2O reductases. The relevant DNA region of about 3.6 kbp was also sequenced and found to consist of four genes, nosDFYL based on the similarity with the N2O reduction genes of Pseudomonas stutzeri. The gene product of A. cycloclastes nosF (299 amino acid residues) has a consensus ATP-binding sequence, and the nos Y gene encodes a hydrophobic protein (273 residues) with five transmembrane segments, suggesting the similarity with an ATP-binding cassette (ABC) transporter which has two distinct domains of a highly hydrophobic region and ATP-binding sites. The nosL gene encodes a protein of 193 amino acid residues and the derived sequence showed a consensus sequence of lipoprotein modification/processing site. The expression of nosZ gene in Escherichia coli cells and the comparison of the translated sequences of the nosDFYL genes with those of bacterial transport genes for inorganic ions are discussed.  (+info)

Nitrate removal in closed-system aquaculture by columnar denitrification. (2/705)

The columnar denitrification method of nitrate-nitrogen removal from high-density, closed system, salmonid aquaculture was investigated and found to be feasible. However, adequate chemical monitoring was found to be necessary for the optimization and quality control of this method. When methanol-carbon was not balanced with inlet nitrate-nitrogen, the column effluent became unsatisfactory for closed-system fish culture due to the presence of excess amounts of nitrite, ammonia, sulfide, and dissolved organic carbon. Sulfide production was also influenced by column maturity and residence time. Methane-carbon was found to be unsatisfactory as an exogenous carbon source. Endogenous carbon could not support high removal efficiencies. Freshwater columns adpated readily to an artificial seawater with a salinity of 18% without observable inhibition. Scanning electron microscopy revealed that the bacterial flora was mainly rod forms with the Peritricha (protozoa) dominating as the primary consumers. Denitrifying bacteria isolated from freshwater columns were tentatively identified as species of Pseudomonas and Alcaligenes. A pilot plant column was found to behave in a manner similar to the laboratory columns except that nitrite production was never observed.  (+info)

Biochemical characterization and solution structure of nitrous oxide reductase from Alcaligenes xylosoxidans (NCIMB 11015). (3/705)

Nitrous oxide reductase (N2OR) is the terminal enzyme involved in denitrification by microbes. No three-dimensional structural information has been published for this enzyme. We have isolated and characterised N2OR from Alcaligenes xylosoxidans (AxN2OR) as a homodimer of M(r) 134,000 containing seven to eight copper atoms per dimer. Comparison of sequence and compositional data with other N2ORs suggests that AxN2OR is typical and can be expected to have similar domain folding and subunit structure to other members of this family of enzymes. We present synchrotron X-ray-scattering data, analysed using a model-independent method for shape restoration, which gave a approximately 20 A resolution structure of the enzyme in solution, providing a glimpse of the structure of any N2OR and shedding light on the molecular architecture of the molecule. The specific activity of AxN2OR was approximately 6 mumol of N2O reduced.min-1. (mg of protein)-1; N2OR activity showed both base and temperature activation. The visible spectrum exhibited an absorption maximum at 550 nm with a shoulder at 635 nm. On oxidation with K3Fe(CN)6, the absorption maximum shifted to 540 nm and a new shoulder at 480 nm appeared. Reduction under anaerobic conditions resulted in the formation of an inactive blue form of the enzyme with a broad absorption maximum at 650 nm. As isolated, the enzyme shows an almost featureless EPR spectrum, which changes on oxidation to give an almost completely resolved seven-line hyperfine signal in the gII region, g = 2.18, with AII = 40 G, consistent with the enzyme being partially reduced as isolated. Both the optical and EPR spectra of the oxidized enzyme are characteristic of the presence of a CuA centre.  (+info)

PCR detection of genes encoding nitrite reductase in denitrifying bacteria. (4/705)

Using consensus regions in gene sequences encoding the two forms of nitrite reductase (Nir), a key enzyme in the denitrification pathway, we designed two sets of PCR primers to amplify cd1- and Cu-nir. The primers were evaluated by screening defined denitrifying strains, denitrifying isolates from wastewater treatment plants, and extracts from activated sludge. Sequence relationships of nir genes were also established. The cd1 primers were designed to amplify a 778 to 799-bp region of cd1-nir in the six published sequences. Likewise, the Cu primers amplified a 473-bp region in seven of the eight published Cu-nir sequences. Together, the two sets of PCR primers amplified nir genes in nine species within four genera, as well as in four of the seven sludge isolates. The primers did not amplify genes of nondenitrifying strains. The Cu primers amplified the expected fragment in all 13 sludge samples, but cd1-nir fragments were only obtained in five samples. PCR products of the expected sizes were verified as nir genes after hybridization to DNA probes, except in one case. The sequenced nir fragments were related to other nir sequences, demonstrating that the primers amplified the correct gene. The selected primer sites for Cu-nir were conserved, while broad-range primers targeting conserved regions of cd1-nir seem to be difficult to find. We also report on the existence of Cu-nir in Paracoccus denitrificans Pd1222.  (+info)

The blue copper-containing nitrite reductase from Alcaligenes xylosoxidans: cloning of the nirA gene and characterization of the recombinant enzyme. (5/705)

The nirA gene encoding the blue dissimilatory nitrite reductase from Alcaligenes xylosoxidans has been cloned and sequenced. To our knowledge, this is the first report of the characterization of a gene encoding a blue copper-containing nitrite reductase. The deduced amino acid sequence exhibits a high degree of similarity to other copper-containing nitrite reductases from various bacterial sources. The full-length protein included a 24-amino-acid leader peptide. The nirA gene was overexpressed in Escherichia coli and was shown to be exported to the periplasm. Purification was achieved in a single step, and analysis of the recombinant Nir enzyme revealed that cleavage of the signal peptide occurred at a position identical to that for the native enzyme isolated from A. xylosoxidans. The recombinant Nir isolated directly was blue and trimeric and, on the basis of electron paramagnetic resonance spectroscopy and metal analysis, possessed only type 1 copper centers. This type 2-depleted enzyme preparation also had a low nitrite reductase enzyme activity. Incubation of the periplasmic fraction with copper sulfate prior to purification resulted in the isolation of an enzyme with a full complement of type 1 and type 2 copper centers and a high specific activity. The kinetic properties of the recombinant enzyme were indistinguishable from those of the native nitrite reductase isolated from A. xylosoxidans. This rapid isolation procedure will greatly facilitate genetic and biochemical characterization of both wild-type and mutant derivatives of this protein.  (+info)

Transcriptional organization of the czc heavy-metal homeostasis determinant from Alcaligenes eutrophus. (6/705)

The Czc system of Alcaligenes eutrophus mediates resistance to cobalt, zinc, and cadmium through ion efflux catalyzed by the CzcCB2A cation-proton antiporter. DNA sequencing of the region upstream of the czcNICBADRS determinant located on megaplasmid pMOL30 revealed the 5' end of czcN and a gene for a MgtC-like protein which is transcribed in the orientation opposite that of czc. Additional open reading frames upstream of czc had no homologs in the current databases. Using oligonucleotide-probed Northern blotting experiments, a 500-nucleotide czcN message and a 400-nucleotide czcI message were found, and the presence of 6, 200-nucleotide czcCBA message (D. Van der Lelie et al., Mol. Microbiol. 23:493-503, 1997) was confirmed. Induction of czcN, czcI, czcCBA, and czcDRS followed a similar pattern: transcription was induced best by 300 microM zinc, less by 300 microM cobalt, and only slightly by 300 microM cadmium. Reverse transcription-PCR gave evidence for additional continuous transcription from czcN to czcC and from czcD to czcS, but not between czcA and czcD nor between czcS and a 131-amino-acid open reading frame following czcS. The CzcR putative response regulator was purified and shown to bind in the 5' region of czcN. A reporter strain carrying a czcNIC-lacZ-czcBADRS determinant on plasmid pMOL30 was constructed, as were DeltaczcR and DeltaczcS mutants of this strain and of AE128(pMOL30) wild type. Experiments on (i) growth of these strains in liquid culture containing 5 mM Zn2+, (ii) induction of the beta-galactosidase in the reporter strains by zinc, cobalt, and cadmium, and (iii) cDNA analysis of czcCBA mRNA synthesis under inducing and noninducing conditions showed that the CzcRS two-component regulatory system is involved in Czc regulation.  (+info)

Re-evaluation of the primary structure of Ralstonia eutropha phasin and implications for polyhydroxyalkanoic acid granule binding. (7/705)

Sequence analysis of several cDNAs encoding the phasin protein of Ralstonia eutropha indicated that the carboxyl terminus of the resulting derived protein sequence is different from that reported previously. This was confirmed by: (1) sequencing of the genomic DNA; (2) SDS-PAGE and peptide analysis of wild-type and recombinant phasin; and (3) mass spectrometry of wild-type phasin protein. The results have implications for the model proposed for the binding of this protein to polyhydroxyalkanoic acid granules in the bacterium.  (+info)

Phospholipid bound to the flavohemoprotein from Alcaligenes eutrophus. (8/705)

The structurally characterized flavohemoprotein from Alcaligenes eutrophus (FHP) contains a phospholipid-binding site with 1-16 : 0-2-cyclo-17 : 0-diacyl-glycerophospho-ethanolamine and 1-16 : 0-2-cyclo-17 : 0-diacyl-glycerophospho-glycerol as the major occupying compounds. The structure of the phospholipid is characterized by its compact form, due to the -sc/beta/-sc conformation of the glycerol and the nonlinear arrangement of the sn-1- and sn-2-fatty acid chains. The phospholipid-binding site is located adjacent to the heme molecule at the bottom of a large cavity. The fatty acid chains form a large number of van der Waal's contacts with nonpolar side chains, whereas the glycerophosphate moiety, which points towards the entrance of the channel, is linked to the protein matrix by polar interactions. The thermodynamically stable globin module of FHP, obtained after cleaving off the oxidoreductase module, also contains the phospholipid and can therefore be considered as a phospholipid-binding protein. Single amino acid exchanges designed to decrease the lipid-binding site revealed both the possibility of blocking incorporation of the phospholipid and its capability to evade steric barriers. Conformational changes in the phospholipid can also be induced by binding heme-ligating compounds. Phospholipid binding is not a general feature of flavohemoproteins, because the Escherichia coli and the yeast protein exhibit less and no lipid affinity, respectively.  (+info)

*Achromobacter xylosoxidans

... (formerly Alcaligenes xylosoxidans) is a Gram-negative, aerobic, oxidase and catalase-positive, ...

*Alcaligenes

... is a genus of Gram-negative, aerobic, rod-shaped bacteria. The species are motile with one or more peritrichous ... Strains of Alcaligenes (such as A. faecalis) are found mostly in the intestinal tracts of vertebrates, decaying materials, ... Alcaligenes species have been used for the industrial production of nonstandard amino acids; A. eutrophus also produces the ... Alcaligenes species have been increasingly recovered over the past decade from patients with cystic fibrosis (CF). An ...

*Alcaligenes piechaudii

... is a bacterium; its type strain is CIP 60.75 (= Hugh 366-5 = IAM 12591 = LMG 1873). It is rod-shaped, ... Kiredjian, M.; Holmes, B.; Kersters, K.; Guilvout, I.; De Ley, J. (1986). "Alcaligenes piechaudii, a New Species from Human ... Peel MM, Hibberd AJ, King BM, Williamson HG (August 1988). "Alcaligenes piechaudii from chronic ear discharge". Journal of ...

*Alcaligenes denitrificans

denitrificans). "Genus Alcaligenes". Bacterio.net. List of prokaryotic names with standing in nomenclature (LSPN). "Alcaligenes ... Alcaligenes denitrificans is a Gram-negative, oxidase- and catalase-positive, strictly aerobic, motile bacterium with ... doi:10.1099/ijs.0.02609-0. "SPECIES Alcaligenes faecalis". UniProt. Georg M. Garrity. Sc.D. (2005). "The Proteobacteria Part C ... Yabuuchi et al propose that Alcaligenes denitrificans should be classified as a subspecies of 'Achromobacter xylosoxidans (A. x ...

*Pseudomonas alcaligenes

... is a Gram-negative aerobic bacterium used for bioremediation purposes of oil pollution, pesticide ... Based on 16S rRNA analysis, P. alcaligenes has been placed in the P. aeruginosa group. O'Mahony, MM; Dobson, AD; Barnes, JD; ... Valenstein, P; Bardy, GH; Cox, CC; Zwadyk, P (1983). "Pseudomonas alcaligenes endocarditis". American Journal of Clinical ... Type strain of Pseudomonas alcaligenes at BacDive - the Bacterial Diversity Metadatabase. ...

*Alcaligenes faecalis

... is a species of Gram-negative, rod-shaped bacteria commonly found in the environment. It was originally ... In 2001, previously unidentified isolates of Alcaligenes were classified as a new subspecies of A. faecalis: A. faecalis ... Type strain of Alcaligenes faecalis at BacDive - the Bacterial Diversity Metadatabase. ... 934-6. Rehfuss, Marc; Urban, James (2005). "Alcaligenes faecalis subsp. Phenolicus subsp. Nov. A phenol-degrading, denitrifying ...

*Alcaligenes venustus

... is a bacterium which has been reclassified to Halomonas venusta. IPSN Bacterio.net Straininfo Alcaligenes ...

*Alcaligenes aestus

... is a bacterium from the genus Alcaligenes. IPSN Bacterio.net Straininfo of Alcaligenes aestus Taxonomy ...

*Alcaligenes pacificus

... is a bacterium which has been reclassified to Deleya pacifica. IPSN Bacterio.net Straininfo of ... Alcaligenes pacificus Taxonomy Browser The Taxonomicon Bergey´s Manual of Determinative Bacteriology ninth Edition John G. Holt ...

*Alcaligenes cupidus

... is a bacterium from the genus Alcaligenes which was isolated from seawater. IPSN Bacterio.net Straininfo of ... Alcaligenes cupidus Taxonomy Browser WoRMS World Register of Marine Species [1] ATCC Taxonomy Browser. ...

*Alcaligenes aquatilis

Alcaligenes aaquatilis is a Gram-negative, catalase- and cytochrome oxidase-positive, motile bacterium with peritrichous ... IPSN Bacterio.net Straininfo of Alcaligenes aquatilis International Journal of Systematic and Evolutionary Microbiology [1] ... flagella, from the genus Alcaligenes, which was isolated in Germany from sediments of the Weser Estuary and in Shem Creek in ...

*Alcaligenes ruhlandii

... first classified as Hydrogenomonas ruhlandii, is a bacterium which has been reclassified to Achromobacter ... IPSN Bacterio.net Straininfo of Alcaligenes ruhlandii IJSEM International Journal of Systematic and Evolutionary Microbiology [ ...

*Alcaligenes viscolactis

... is a bacterium which can produce ropiness in milk and which can grow in sun tea. [1] "Alcaligenes ...

*Alcaligenes defragrans

... motile bacterium from the genus Alcaligenes. IPSN Bacterio.net Straininfo of Alcaligenes defragrans Taxonomy Browser ... Alcaligenes defragrans is a Gram-negative, oxidase- and catalase-positive, strictly aerobic, ...

*Indole test

Alcaligenes sp., most Bacillus sp., Bordetella sp., Enterobacter sp., most Haemophilus sp., most Klebsiella sp., Neisseria sp ...

*Extracellular polymeric substance

stewartii) succinoglycan (Alcaligenes faecalis var myxogenes, Sinorhizobium meliloti) xanthan (Xanthomonas campestris) welan ( ... Alcaligenes spp.) Extracellular matrix in multi-cellular organisms Staudt C, Horn H, Hempel DC, Neu TR (2004). "Volumetric ... Alcaligenes faecalis var. myxogenes) cyclosophorans (Agrobacterium spp., Rhizobium spp. and Xanthomonas spp.) dextran ( ... Alcaligenes viscosus, Zymomonas mobilis, Bacillus subtilis) pullulan (Aureobasidium pullulans) scleroglucan (Sclerotium rolfsii ...

*Plastocyanin family of copper-binding proteins

... blue copper protein from Alcaligenes faecalis; cupredoxin (CPC) from Cucumis sativus (Cucumber) peelings; cusacyanin (basic ...

*Cupriavidus necator

Schneider, K.; Cammack, R.; Schlegel, G. & Hall, D. (1979). "THE IRON-SULPHUR CENTRES OF SOLUBLE HYDROGENASE FROM ALCALIGENES ... H. eutrophus was then renamed Alcaligenes eutropha because it was a micro-organism with degenerated peritrichous flagellation. ... 1995). "Transfer of two Burkholderia and an Alcaligenes species to Ralstonia gen. nov.: proposal of Ralstonia pickettii ( ... Lenz, O.; Friedrich, B. (1998). "A novel multicomponent regulatory system mediates H2 sensing in Alcaligenes eutrophus". PNAS. ...

*1-hydroxy-2-naphthoate hydroxylase

Deveryshetty, J.; Phale, P.S. (2010). "Biodegradation of phenanthrene by Alcaligenes sp. strain PPH: partial purification and ...

*Comamonadaceae

for Alcaligenes paradoxus (Davis 1969). 1991. International Journal of Systematic Bacteriology. 41: 445-450 PDF Online Garrity ...

*Variovorax paradoxus

... were originally classified in the genera Alcaligenes along with V. paradoxus (Alcaligenes eutrophus and Alicaligenes paradoxus ... nov., for Alcaligenes paradoxus (Davis 1969)". International Journal of Systematic Bacteriology. 41 (3): 445-450. doi:10.1099/ ...

*Pseudomonas pseudoalcaligenes

Monias, B. L. (1928). "Classification of Bacterium alcaligenes pyocyaneum and fluorescens". J Infect Dis. 43 (4): 330-334. doi: ...

*4-Hydroxybenzoic acid

It can be found in Alcaligenes sp. The enzyme 4-chlorobenzoate dehalogenase uses 4-chlorobenzoate and H2O to produce 4- ...

*N-acyl-D-amino-acid deacylase

Wakayama M, Hayashi S, Yatsuda Y, Katsuno Y, Sakai K, Moriguchi M (1996). "Overproduction of D-aminoacylase from Alcaligenes ... "Cloning and sequencing of a gene encoding D-aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6 and expression ...

*Methylguanidinase

Nakajima M, Shirokane Y, Mizusawa K (1980). "A new amidinohydrolase, methylguanidine amidinohydrolase from Alcaligenes sp. N-42 ...
Role of arginine residues of D-aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6.: To investigate the role of arginine in the folding of d-amin
Gentaur molecular products has all kinds of products like :search , AbD \ GOAT ANTI ALCALIGENES SPP CHOLINE OXIDASE, Product Type Polyclonal Antibody, Specificity CHOLINE OXIDASE, Target Species Bacterial, Host Goat, Format Purified, Isotypes Polyclonal IgG, Applicati \ 2080-5050 for more molecular products just contact us
Studies on the anaerobic and aerobic growth of Alcaligenes eutrophus bacteria show that the same growth factor suffice for both aerobic and anaerobic growth, thus supporting Mathava and Ligy formulation of photosynthesis and carbon assimilation in these forms, and his thesis that the previously observed need for peptone or yeast extract could be attributed to their content of essential growth factors. A bottle technique for anaerobic cultures and a flask technique for aerobic culture are described in detail. Photosynthesis in A. eutrophus bacteria is best observed when they are grown anaerobically. The experiments described here indicate that the culture media had to be modified to conform to certain special requirement imposed by anaerobic growth; the required growth factors remained the same. A. eutrophus bacteria display the same growth pattern in both aerobic and anaerobic condition in ethylene substrate while there were some slight changes of its growth pattern in both aerobic and anaerobic ...
1ET7: Catalytic roles for two water bridged residues (Asp-98 and His-255) in the active site of copper-containing nitrite reductase.
1l9s: Directing the mode of nitrite binding to a copper-containing nitrite reductase from Alcaligenes faecalis S-6: characterization of an active site isoleucine.
TWENTY FIVE Aspergillus isolates were screened from Giza Governorate and Saint Catherine Protectorate soils in Egypt. The antimicrobial activity of the crude extracts was tested against two Gram positive bacteria (Bacillus subtilis NRRL-B-4219, Staphylococcus aureus ATCC29213), four Gram negative bacteria (Alcaligenes faecalis B-170, Escherichia coli ATCC25922, Klebsiella pneumoniae ATCC10131, Pseudomonas aeruginosa ATCC27953), and one yeast (Candida albicans ATCC10231). The antioxidant activity using free radical scavenging model was assayed for the crude extracts. The antitumor activity for all of crude extracts was determined against HCT116 (Colon carcinoma cell line), HEPG2 (Liver carcinoma cell line), and MCF-7 (Breast carcinoma cell line). Aspergillus nomius was the most potent fungal species accordingly, it was chosen for bioactivity assay. Identification of this species was further confirmed at the molecular level based on nuclear ribosomal DNA 18s identities. An accession number, LC199488, was
1GS8: Biochemical and Crystallographic Studies of the met144Ala, Asp92Asn and His254Phe Mutants of the Nitrite Reductase from Alcaligenes Xylosoxidans Provide Insight Into the Enzyme Mechanism.
Alcaligenes é um gênero de bactérias gram-negativas aeróbicas da família Alcaligenaceae. Madigan M; Martinko J (editors). (2005). Brock Biology of Microorganisms 11th ed. ed. [S.l.]: Prentice Hall. ISBN 0-13-144329-1 !CS1 manut: Texto extra (link) Alcaligenes - Kenyon College ...
Eight strains of hydrogen bacteria belonging to the genera Hydrogenomonas, Pseudomonas, Nocardia, and to coryneform bacteria have been analyzed with respect to the cytochrome patterns. All strains...
If you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library, or send a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. ...
Alcaligenes Polysaccharides are manufactured by Hakuto under the tradename "Alcasealan", and probably distributed in Europe by Nagase. It is described as a "natural polysaccharides produced from fermentation. The polymer chain of Alcasealan forms a three-dimension network to hold free water as well as binding it. This 3D network has a unique characteristic which forms a moisturising film on the skin with unique lightweight texture. Alcasealan is widely used as emulsion and dispersion stabiliser; it also helps to suspense powders and glitters in cosmetics formulations. ...
This is a water gelling agent made from a Gram-negative bacteria, similar to xanthan gum.. A 0.001% concentration can thicken up a product quite nicely, which is good because it costs more than $10 000 for a kilo.. Beyond thickening water, it also has moisturizing properties, similar to hyaluronic acid.. Is it better or worth the money? Im not sure - but if the popularity of this ingredient increases, the cost will go down. Much of the cost is the low yield from production. You can read more about how its produced here.. Japanese and Korean suppliers tend to have many unique and interesting raw materials, from my experience.. Its found in a few commercial products, which are very luxury and expensive (as is to be expected).. ...
DI-fusion, le Dépôt institutionnel numérique de lULB, est loutil de référencementde la production scientifique de lULB.Linterface de recherche DI-fusion permet de consulter les publications des chercheurs de lULB et les thèses qui y ont été défendues.
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Copper-containing nitrite reductases (NiRs) are enzymes that efficiently reduce nitrite to nitric oxide in potent denitrifying bacteria. There has been an interest in their application in amperometric biosensors for monitoring nitrite levels in natural and waste waters. NiRs have a complex enzyme mechanism and depend on nitrite concentration and pH. Although the mechanism has been intensively studied, it is still controversial. In this thesis, a combined fluorescence and electrochemical method is used to simultaneously monitor the nitrite turn-over rate of a NiR from Alcaligenes faecalis S-6 and the oxidation state of the type-1 copper electron transfer site inside the enzyme. The catalytic activity of NiR is measured electrochemically by exploiting a direct electron transfer to fluorescently labelled enzyme molecules immobilised on modified gold, whereas the redox state of the type-1 copper site is determined from fluorescence intensity changes caused by F6rster resonance energy transfer (FRET) ...
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2-Aminobenzenesulphonic acid (2AS) is degraded by Alcaligenes sp. strain O-1 via a previously detected but unidentified intermediate. A mutant of strain O-1 was found to excrete this intermediate, which was isolated and identified by m.s., 1H- and 13C-n.m.r. as 3-sulphocatechol (3SC). Proteins from cell extracts of strain O-1 were separated by anion-exchange chromatography. A multicomponent oxygenase was observed to convert 1 mol each of NADH, O2 and 2AS into 1 mol each of 3SC, NH3 and NAD+. The enzyme presumably catalysed formation of the ring of a 2-amino-2,3-diol moiety, and elimination in the amino group led to a rearomatization. 3SC was further degraded via meta ring cleavage, which could be prevented by inactivation of the 3-sulphocatechol-2,3-dioxygenase (3SC23O) with 3-chlorocatechol. In Tris buffer, the separated 3SC23O catalysed the reaction of 1 mol each of 3SC and O2 involving a transient yellow intermediate, and release of 1 mol of sulphite and two organic products. The major ...
Alcaligenes faecalis subsp. faecalis ATCC ® 8750™ Designation: 16 TypeStrain=True Application: Produces nitrilase Quality control strain Quality control strain for Autobac products
Alcaligenes faecalis subsp. faecalis ATCC ® 35655™ Designation: LRA 41 02 82 TypeStrain=False Application: Quality control strain Quality control strain for API products
The nickel resistance determinant ncrABCY was identified in Leptospirillum ferriphilum UBK03. Within this operon, ncrA and ncrC encode two membrane proteins that form an efflux system, and ncrB encodes NcrB, which belongs to an uncharacterized family (DUF156) of proteins. How this determinant is regulated remains unknown. Our data indicate that expression of the nickel resistance determinant is induced by nickel. The promoter of ncrA, designated pncrA, was cloned into the promoter probe vector pPR9TT, and co-transformed with either a wild-type or mutant nickel resistance determinant. The results revealed that ncrB encoded a transcriptional regulator that could regulate the expression of ncrA, ncrB, and ncrC. A GC-rich inverted repeat sequence was identified in the promoter pncrA. Electrophoretic mobility shift assays (EMSAs) and footprinting assays showed that purified NcrB could specifically bind to the inverted repeat sequence of pncrA in vitro; this was confirmed by bacterial one-hybrid analysis.
The diversity of 2,4-dichlorophenoxyacetic acid (2,4-D)-degradative plasmids in the microbial community of an agricultural soil was examined by complementation. This technique involved mixing a suitable Alcaligenes eutrophus (Rifr) recipient strain with the indigenous microbial populations extracted from soil. After incubation of this mixture, Rifr recipient strains which grow with 2,4-D as the only C source were selected. Two A. eutrophus strains were used as recipients: JMP228 (2,4-D-), which was previously derived from A. eutrophus JMP134 by curing of the 2,4-D-degradative plasmid pJP4, and JMP228 carrying pBH501aE (a plasmid derived from pJP4 by deletion of a large part of the tfdA gene which encodes the first step in the mineralization of 2,4-D). By using agricultural soil that had been treated with 2,4-D for several years, transconjugants were obtained with both recipients. However, when untreated control soil was used, no transconjugants were isolated. The various transconjugants had plasmids
A Megaplasmid-Borne Anaerobic Ribonucleotide Reductase in Alcaligenes eutrophus H16: The conjugative 450-kb megaplasmid pHG1 is essential for the anaerobic grow
The biopharmaceutical industry needs faster and more efficient development of new drugs and their market introduction as well as shorter process development times for both upstream and downstream operations. It has become more commonplace to use high-throughput development techniques to save time (1). Development is also sped up by applying platform technologies based on the unsurpassed selectivity of protein A resins (2,3,4,5,6), which is the foundation for downstream processing of monoclonal antibodies (MAbs). This is the second of two articles…. ...
Table 7 lists the aerobic bioprocesses previously conducted in an OBR.. Alcaligenes eutrophus H16 has commercial interest for production of the biodegradable plastic poly-β-hydroxybutyrate (PHB). This bacterium has been cultured in an OBR [79] with a maximum specific growth rate (μmax) of 0.39 h−1 compared with 0.36 h−1 and 0.35 h−1 when using Erlenmeyer flasks at 10 per cent and 40 per cent working volumes, respectively. OBRs are, therefore, suitable for culturing rapidly growing, oxygen demanding micro-organisms. The same paper alluded to the use of OBRs for culturing animal cells: an interesting proposal as they are notoriously shear-sensitive [92] with cell death being proportional to energy input; so any scaled-up reactor should minimize energy input [43]. Bioprocesses containing animal cells could greatly benefit from the low shear, high mass transfer environment produced in OBRs to minimize shear while maintaining sufficient OTRs.. The fruity, peach-like aroma compound ...
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Pelomonas saccharophila es una bacteria Gram-negativa del suelo, una de las 3 especies descritas del género Pelomonas. Xie C.H. & Yokota A., Reclassification of Alcaligenes latus strains IAM 12599T and IAM 12664 and Pseudomonas saccharophila as Azohydromonas lata gen. nov., comb. nov., Azohydromonas australica sp. nov. and Pelomonas saccharophila gen. nov., comb. nov., respectively, Int. J. Syst. Evol. Microbiol., vol. 55, p. 2419-2425, 2005 Pelomonas en LPSN Bacterio.net - List of Prokaryotic names with Standing in Nomenclature, consultado el 30 de septiembre de ...
In nature a significant part of the microbial activity is concentrated at or near oxic/anoxic interfaces, where oxygen concentrations are often low. Bacteria possessing different kinetic characteristics for oxygen and employing distinct metabolic pathways for the degradation of (halo)aromatic substrates for which oxygen is needed as co-substrate may have to compete with each other in such environments. In this study the competitiveness of Pseudomonas sp. strain A3 relative to Alcaligenes sp. strain L6 was tested in batch and in continuous cultures. While both of these strains are able to metabolise 3-chlorobenzoate (3CBA), the former was isolated under air saturating conditions and employs the catechol pathway, whereas the latter was isolated under reduced partial pressures of oxygen and was capable of metabolising 3CBA via the gentisate pathway. Competition experiments in batch culture resulted in pure cultures of Pseudomonas sp. strain A3 under air saturating conditions. However, if reduced ...
Background and Aims: Food is of paramount importance to the sustenance of human health, on that basis this study was initiated with a view to determining bacterial agents associated with the vended foods and to investigating antibacterial resistance of the isolates. Methods: Twelve food samples from vendors were randomly collected from ten locations within Lagos environs. The food samples were analyzed bacteriologically by standard methods. The isolates were identified and subjected to antimicrobial susceptibility testing using standard procedures. Results: The total plate counts of bacteria recorded was between 2.98 × 103 cfu/g in Jollofrice and 4.09 × 104 cfu/g in Salad. Seventy-six bacterial isolates made up of six genera; Escherichia coli, Salmonella Typhimurium, Alcaligenes spp., Klebsiella pneumoniae, Enterobacter spp., Serratia marcensens and Proteus spp. were identified. Most of the bacterial isolates were moderately sensitive to cotrimoxazole, nalidixic acid, amoxicillin and nitrofurantion
strict aerobe by thioglyoclate test, negative carb fermentation, positive citrate, negative indole, positive urease, catalase positive, gram negative rod,positive decarboxylation tests......I think I have Alcaligenes faecalis or pseudomonas aerugenosa. Motility was positive from the deep stab. I think my test results match those organism except for the positive urease ...
strict aerobe by thioglyoclate test, negative carb fermentation, positive citrate, negative indole, positive urease, catalase positive, gram negative rod,positive decarboxylation tests......I think I have Alcaligenes faecalis or pseudomonas aerugenosa. Motility was positive from the deep stab. I think my test results match those organism except for the positive urease ...
bacteria HoxJ protein: putative histidine protein kinase and molecular hydrogen sensor; GenBank U82565 (Alcaligenes hydrogenophilus)
Dodgson, K.S., Spencer, B. and Williams, K. (1956). „Studies on sulphatases. 13. The hydrolysis of substituted phenyl sulphates by the arylsulphatase of Alcaligenes metacaligenes". Biochem. J. 64: 216-221. PMID 13363831 ...
Achromobacter xylosoxidans (formerly Alcaligenes xylosoxidans) is a Gram-negative, aerobic, oxidase and catalase-positive, motile bacterium with peritrichous flagella, from the genus Achromobacter. It is generally found in wet environments. Achromobacter xylosoxidans can cause infections such as bacteremia, especially in patients with cystic fibrosis. In 2013, the complete genome of an A. xylosoxidans strain from a patient with cystic fibrosis was sequenced. A. xylosoxidans is a Gram-negative rod that does not form spores. It is motile, with peritrichous flagella that distinguish it from Pseudomonas species, and is oxidase-positive, catalase-positive, and citrate-positive. It is urease and indole-negative. It produces acid oxidatively from xylose, but not from lactose, maltose, mannitol, or sucrose. It grows well on MacConkey agar and other inhibitory growth media such as deoxycholate, Salmonella-Shigella, and nalidixic acid-cetrimide agars. It is usually resistant to a variety of antibiotics ...
Achromobacter xylosoxidans is a ubiquitous Gram-negative non-fermenting rod, recently characterized as an emerging pathogen in cystic fibrosis (CF) patients. Its pathogenic potential and prevalent transmission routes are still unclear. This study investigated the PFGE genetic pattern and antimicrobial resistance profile of 42 A. xylosoxidans isolates obtained over 4 years from the respiratory tract of 22 CF patients. By genotypic analysis, 31 isolates were attributed to 8 distinct PFGE patterns (A-H), whereas 11 isolates were not typable because their DNA was not restricted by XbaI and DraI restriction enzymes. The majority of the isolates showed multidrug resistance; imipenem and piperacillin were the most active drugs. During the course of A. xylosoxidans chronic infection forced expiratory volume and body mass index values were not significantly lowered. The demonstration of widespread antibiotic resistance underscores the importance of antibiogram-directed therapy. Our data suggest that in ...
Hydrogen gas is a potential renewable alternative energy carrier that could be used in the future to help supplement humanitys growing energy needs. Unfortunately, current industrial methods for hydrogen production are expensive or environmentally unfriendly. In recent years research has focused on biological mechanisms for hydrogen production and specifically on hydrogenases, the enzyme responsible for catalyzing the reduction of protons to generate hydrogen. In particular, a better understanding of this enzyme might allow us to generate hydrogen that does not use expensive metals, such as platinum, as catalysts. The soluble hydrogenase I (SHI) from the hyperthermophile Pyrococcus furiosus , a member of the euryarchaeota, has been studied extensively and used in various biotechnological applications. This review summarizes the strategies used in engineering and characterizing three different forms of SHI and the properties of the recombinant enzymes. SHI has also been used in in vitro systems ...
To the Editor: The carbapenems (meropenem and imipenem), the ß-lactams with the broadest spectrum, are stable to most ß-lactamases (1). Therefore, they are often used as antibiotics of last resort for treating nosocomial infections due to gram-negative bacteria resistant to other ß-lactams. Resistance to carbapenems and susceptibility to other ß-lactams in Pseudomonas aeruginosa is common as a result of reduced drug accumulation or increased expression of pump efflux (1).. Several extended-spectrum ß-lactamases have been reported in P. aeruginosa, but only two, IMP-1 and VIM-1, possess an extended hydrolysis profile that includes carbapenems (2-5). The chromosome-borne and plasmid-mediated carbapenem-hydrolyzing ß-lactamase, IMP-1, has been described in several gram-negative rods, including P. aeruginosa, P. cepacia, Alcaligenes xylosoxydans, and Enterobacteriaceae isolates in Japan (4,6). Recently, a chromosome-borne carbapenem-hydrolyzing ß-lactamase, VIM-1, was reported from a clinical ...
1.Chen GQ, Wu Q. 2005. The application of polyhydroxyalkanoates as tissue engineering materials. Biomaterials. 26:6565-6578 2. Doi Y, Kunioka M, Nakamura Y, Soga K. 1986. Nuclear magnetic resonance studies on poly(B-hydroxybutyrate) and a copolyester of B-hydroxybutyrate and B-hydroxyvalerate isolated from Alcaligenes eutrophus H16. Macromolecules. 19:2860-2864 3. Endy D. 2005. Foundations for engineering biology. Nature. 438(7067):449-53 4. International Genetically Engineered Machines competition. 15 Jun 2008. 26 Jul 2008. ,http://igem.org, 5. Holmes PA. 1985. Applications of PHB - a microbially produced biodegradable thermoplastic. Physics in technology. 16:32-36 6. Kang Z, Wang Q, and H Zhang. 2008. Construction of a stress-induced system in Escherichia coli for efficient polyhydroxyalkanoates production. Biotechnological Products and Process Engineering. 79:203-208 7. Knight TF. 2003. Idempotent Vector Design for Standard Assembly of BioBricks. Tech. rep., MIT Synthetic Biology Working ...
Bacteria capable of degrading polymeric products were isolated from several sources including tap water, sediment, and a deteriorated polymeric product. Alcaligenes xylosoxidans; T2, Pseudomonas aeruginosa GP10, and Nocardia corynebacterioides S3 were able to utilize rubber products as a sole source of carbon and energy. These microorganisms showed different growth patterns in mineral salt media (MSM) supplemented with rubber strips or with the rubber extract. A. xylosoxidans T2, P. aeruginosa GP10 and N. corynebacterioides S3 reduced the weight of the rubber product by approximately 2.0, 4.0 and 5.3%, respectively, after 70 days of incubation with the rubber product in MSM. On average, 0.45 mg (water-soluble carbon) g(-1) of the rubber product was released into solution phase after 7 days of incubation. Growth of N. corynebacterloides S3 was initially slower but exceeded the other two bacteria upon colonization of the polymer products. N. corynebacterioides S3 formed a dense biofilm on surfaces ...
Sri Kumalaningsih, Nur Hidayat and Nur Aini Abstract Study to obtain the optimum condition for the production of PHAs by Alcaligenes latus was carried using liquid bean curd waste as low cost carbon source. A Response surface method with two factors i.e. the initial sucrose concentration (15 g/L, 20 g/L, and 25 g/L) and time…
Design of Enzyme Reactor. Geoff Greer Joseph Gvildys Aaron McGregor April 2 nd , 2007. Enzyme Selection. Transpeptidation Trypsin or Achromobacter lyticus proteinase I Optimal enzyme conditions 4 o C and a pH of 7. Eli Lilly. Enzyme Immobilization. Benefits Slideshow 209542 by...
... A number of bacteria, e.g., Alcaligenes eutrophus, have been employed to produce large amounts of intracellular polyester, poly-β-hydroxy butyrate or valerate. These polymer mixtures or copolymers are only sparingly soluble in water, and are stable between pH 3-11 under 10-100°C. These polymers are found to be ideal binders for soil that the strength of soil can be enhanced. For porous media such as sand or clay the permeability can be reduced to almost one million fold; therefore, the bacteria derived polymers are perfect plugging agents for stopping the plumes of subsurface pollutants. This is obviously usethl for on-site contaminant-control for hazardous wastes or chemicals. The microbial polymers, including the extracellulosic polymer, can be used as selective plugging agents, so that the concentration of pollutant can be partitioned (or bio-barrier) in different zones and pockets to allow bioremediation to follow (zonal bioremediation
This article is within the scope of WikiProject Microbiology, a collaborative effort to improve the coverage of Microbiology on Wikipedia. If you would like to participate, please visit the project page, where you can join the discussion and see a list of open tasks ...
ID PHG153 preliminary; circular DNA; SYN; 4381 BP. XX AC IG9825; XX DT 01-JUL-1995 (Rel. 11, Created) DT 01-JUL-1995 (Rel. 12, Last updated, Version 1) XX DE E. coli plasmid vector pHG153 - complete. XX KW cloning vector. XX OS Cloning vector OC Artificial sequences; Cloning vehicles. XX RN [1] RC pHG134 from pBR322 & linker RC pHG138 from pUC8 & linker RC pHG140 from pUC8 & linker RC pHG144 from pBR322 & linker RC pHG146 from pUC8 & linkers RC pHG153 from pBR322 & linkers RC pHG155 from pHG153 RC pHG161 from pBR322 & linker RC pHG163 from pHG155 RC pHG165 from pHG163 & pHG146 RC pHG171 from pHG165 & pUC9 RC pHG173 from pHG165 & linker RC pHG175 from pBR322 & linkers RC pHG177 from pHG175 RC pHG179 from pHG165 & pHG175 RC pHG327 from pHG165 & pUC18 RC pHG329 from pHG165 & pUC19 RC pHG331 from pHG146 & pUC18 RC pHG333 from pHG146 & pUC19 RC pHG335 from pHG146 & pUC9 RA Stewart G.S., Lubinsky-Mink S., Jackson C.G., Cassel A., Kuhn J.; RT "pHG165: a pBR322 copy number derivative of pUC8 for cloning ...
3: Gao, X., C.L. Tan, C.C. Yeo and C.L. Poh, 2005. Molecular and biochemical characterization of the xlnD-encoded 3-hydroxybenzoate 6-hydroxylase involved in the degradation of 2,5-xylenol via the gentisate pathway in Pseudomonas alcaligenes NCIMB 9867. J. Bacteriol., 187: 7696-7702 ...
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Looking for online definition of Achromobacter xylosoxidans in the Medical Dictionary? Achromobacter xylosoxidans explanation free. What is Achromobacter xylosoxidans? Meaning of Achromobacter xylosoxidans medical term. What does Achromobacter xylosoxidans mean?
Biological degradation of phenol by Alcaligenes faecalis with high biodegradation activity and high tolerance was investigated at 25C. Phenol could be utilized by the bacteria as the sole carbon and energy sources. The cell growth and substrate degradation of phenol as single substrates for Alcaligenes faecalis in batch cultures (shaking flasks) were investigated at different initial phenol concentrations. Phenol was observed to be an inhibitory compound. Particularly, when free cells grew on a high concentration of phenol, substrate inhibition was observed and the higher the concentration of phenol, the longer was the lag period. The lag time and whole required time for phenol biodegradation was considerably decreased by immobilized cells due to improvement of cells resistance against high Phenol concentration by cell immobilization compared to free cells. Required time for completely degrade initial phenol of 700 and 1000 ppm by free and immobilized cells is 56, 101 and 35, 72 hours ...
Looking for online definition of Achromobacter cobalamini in the Medical Dictionary? Achromobacter cobalamini explanation free. What is Achromobacter cobalamini? Meaning of Achromobacter cobalamini medical term. What does Achromobacter cobalamini mean?
Studies in intermolecular electron transfer between the copper-containing proteins azurin I and nitrate reductase from Alcaligenes xylosoxidans ...
Ralstonia eutropha strain E2 (previously Alcaligenes sp.) is a phenol-degrading bacterium expressing phenol-oxygenating activity with a low Ks (the apparent half-saturation constant in Haldane's equation) and an extremely high KSI (the apparent inhibition constant). To identify the molecular basis for these novel cellular kinetic properties, a 9.5 kb DNA fragment that allowed Pseudomonas aeruginosa PAO1c (Phl- Cat+) to grow on phenol as the sole carbon source was cloned from strain E2 into plasmid pRO1614. PAO1c harbouring this plasmid (designated pROE217) transformed phenol to catechol, indicating that this fragment contains gene(s) for phenol hydroxylase. The cloned genes consist of eight complete ORFs, designated poxRABCDEFG. The products are homologous to those of dmpRKLMNOPQ of Pseudomonas sp. CF600, sharing 30--65% identity: this suggests that the phenol hydroxylase is a multicomponent enzyme. The kinetic constants for phenol-oxygenating activity of PA01c(pROE217) were determined, and these
In Pseudomonas aeruginosa, quorum sensing (QS) regulates the production of secondary metabolites, many of which are antimicrobials that impact on polymicrobial community composition. Consequently, quenching QS modulates the environmental impact of P. aeruginosa. To identify bacteria capable of inactivating the QS signal molecule 2-heptyl-3- hydroxy-4(1H)-quinolone (PQS), a minimal medium containing PQS as the sole carbon source was used to enrich a Malaysian rainforest soil sample. This yielded an Achromobacter xylosoxidans strain (Q19) that inactivated PQS, yielding a new fluorescent compound (I-PQS) confirmed as PQS-derived using deuterated PQS. The I-PQS structure was elucidated using mass spectrometry and nuclear magnetic resonance spectroscopy as 2-heptyl-2-hydroxy-1,2-dihydroquinoline- 3,4-dione (HHQD). Achromobacter xylosoxidans Q19 oxidized PQS congeners with alkyl chains ranging from C1 to C5 and also N-methyl PQS, yielding the corresponding 2-hydroxy-1,2-dihydroquinoline-3,4- diones, ...
Ralstonia metallidurans, formerly known as Alcaligenes eutrophus and thereafter as Ralstonia eutropha, is a beta-Proteobacterium colonizing industrial sediments, soils or wastes with a high content of heavy metals. The type strain CH34 carries two large plasmids (pMOL28 and pMOL30) bearing a variety of genes for metal resistance. A chronological overview describes the progress made in the knowledge of the plasmid-borne metal resistance mechanisms, the genetics of R.
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Methyl 4-fluorobenzoate 403-33-8 MSDS report, Methyl 4-fluorobenzoate MSDS safety technical specifications search, Methyl 4-fluorobenzoate safety information specifications ect.
ID W8WYL2_CASDE Unreviewed; 561 AA. AC W8WYL2; DT 14-MAY-2014, integrated into UniProtKB/TrEMBL. DT 14-MAY-2014, sequence version 1. DT 25-OCT-2017, entry version 19. DE SubName: Full=Glycerol-3-phosphate dehydrogenase {ECO:0000313,EMBL:CDM24848.1}; DE EC=1.1.5.3 {ECO:0000313,EMBL:CDM24848.1}; GN ORFNames=BN940_11951 {ECO:0000313,EMBL:CDM24848.1}; OS Castellaniella defragrans 65Phen. OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; OC Alcaligenaceae; Castellaniella. OX NCBI_TaxID=1437824 {ECO:0000313,EMBL:CDM24848.1, ECO:0000313,Proteomes:UP000019805}; RN [1] {ECO:0000313,EMBL:CDM24848.1, ECO:0000313,Proteomes:UP000019805} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=65Phen {ECO:0000313,EMBL:CDM24848.1}; RX PubMed=24952578; DOI=10.1186/1471-2180-14-164; RA Petasch J., Disch E.M., Markert S., Becher D., Schweder T., Huttel B., RA Reinhardt R., Harder J.; RT "The oxygen-independent metabolism of cyclic monoterpenes in RT Castellaniella defragrans 65Phen."; RL BMC ...
Proteome IDi ,p>The proteome identifier (UPID) is the unique identifier assigned to the set of proteins that constitute the ,a href="http://www.uniprot.org/manual/proteomes_manual">proteome,/a>. It consists of the characters UP followed by 9 digits, is stable across releases and can therefore be used to cite a UniProt proteome.,p>,a href=/help/proteome_id target=_top>More...,/a>,/p> ...
Black dots contaminant phenonmenon: helpful paper - posted in Tissue and Cell Culture: Dear all,It seems like many researcher faced with this bizarre black dancing dots in cell culture, and me too! So Ive search about this issue and found one paper that published in Biologicals 2010. Gray JS, Birmingham JM, Fenton JI.Got black swimming dots in your cell culture? Identification of Achromobacter as a novel cell culture contaminant Biologicals. 2010 Mar;38(2):273-7. Epub 2009 Nov 18....
P:[email protected]; Asm Call P; End; Works like a charm P:[email protected]; Asm Jmp P; End; Whats the difference betwen Jmp and Call? Are they not the same thing?
Di*late .] [L. dilatare; either fr. di- = dis- + latus wide, not the same word as latus, used as p. p. of ferre to bear (see Latit...
TABLE-US-00004 TABLE 4 (MIC value (unit: ppm)) Embodiment 1 Comparison A Comparison B Bacteria 1 Alcaligenes faecalis 1 8 80 2 Alcaligenes viscolactis 1 8 80 3 Ascophyta pisi 10 4 Autotrophic bacteria 20 5 Aster yellows 1 6 Acinetobacter calcoaceticus 4 7 Achrcmobacter gulyatus 1 8 Aerobacter aerogenes 1 9 Aerobacter cloacae 1 8 80 10 Blastomyces italicum 1 11 Bacillus cereus 1 8 80 12 Bacillus mycoides 1 8 80 13 Bacillus subtillis 10 10 80 14 Bacillus megaterrium 10 10 80 15 Bacillus anthracis 10 10 80 16 Bacillus punctatum 10 10 80 17 Bacterium vulgaro 1 18 Bacterium pyocyaneum 1 19 Blastomyces deematidis 1 20 Bacterroid fragilis 3 21 Campylobacter fetus 3 22 Clostridium perfringens 3 23 Clostridium difficile 3 24 Corticium fuciforme 3 25 Clostridium botulinum 3 26 Cloechera apiculata 10 27 Cellulomonas iugis 1 28 Campylobacter jejuni/coli 10 29 Dactylium dendroides 3 30 Diplodia viticol 3 31 Debaryamyces hansenii 15 32 Desulfovibrio desullfuricans 1 33 Endothia paracitica 1 34 Escherichia ...
2) 5,6-dihydrothymine + NAD+ = thymine + NADH + H+. For diagram of reaction click here.. Other name(s): dihydropyrimidine dehydrogenase; dihydrothymine dehydrogenase; pyrimidine reductase; thymine reductase; uracil reductase; dihydrouracil dehydrogenase (NAD+). Systematic name: 5,6-dihydropyrimidine:NAD+ oxidoreductase. Comments: An iron-sulfur flavoenzyme. The enzyme was originally discovered in the uracil-fermenting bacterium, Clostridium uracilicum, which utilizes uracil and thymine as nitrogen and carbon sources for growth [1]. Since then the enzyme was found in additional organisms including Alcaligenes eutrophus [2], Pseudomonas strains [3,4] and Escherichia coli [5,6].. Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number: 9026-89-5. References:. 1. Campbell, L.L. Reductive degradation of pyrimidines. III. Purificaion and properties of dihydrouracil dehydrogenase. J. Biol. Chem. 227 (1957) 693-700. [PMID: 13462991]. 2. Schmitt, U., Jahnke, K., Rosenbaum, K., Cook, ...
BioMed Research International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies covering a wide range of subjects in life sciences and medicine. The journal is divided into 55 subject areas.
Ninkovic M., Mitkovski M, Kohl T, Pardo LA, Stühmer W: Physical and functional interaction of KV10.1 with rabaptin-5 impacts ion channel trafficking (in preparation). Mitrikeski PT, Gjuracic K, Koren P, Lisnic B, Miklenic M, Ninkovic M, Stafa A, Svetec IK: Short history of yeast genetics in Croatia (submitted). Ninkovic M: DNA recombination using StEP. Evolutionary methods in biotechnology.Eds: Brakmann, Schwienhorst. WILEY-VCH GmbH & Co. KGaA, 2004, 25-30. Ninkovic M, Hildmann C, Dietrich R, Wegner D, Zimmermann T, Birch OM, Bernegger C, Loidl P, Schwienhorst A: A novel amidohydrolase from Bordatella/Alcaligenes strain FB188 with similarities to histone deacetylases. Journal of Bacteriology 2004: 186(8): 2328-39. Ninkovic M, Dietrich R, Schwienhorst A: Advances in DNA recombination technology. Biotech News 2001: 6(6):14-15. Ninkovic M, Riester D, Wirsching F, Dietrich R, Schwienhorst A: Fluorogenic assay for penicillin acylase activity. Anal Biochem 2001: 292: 228-233. Ninkovic M, Dietrich R, ...
A novel gluconate dehydratase derived from |i|Achromobacter xylosoxidans |/i|and a gene encoding the gluconate dehydratase are provided. By reacting the gluconate dehydratase or a transformed cell con
Alcaligenaceae é uma família de bactérias da ordem Burkholderiales. Os seguintes gêneros são reconhecidos para a família: Achromobacter Yabuuchi & Yano 1981 Advenella Coenye, Vanlaere, Samyn, Falsen, Larsson & Vandamme 2005 Alcaligenes Castellani & Chalmers 1919 Azohydromonas Xie & Yokota 2005 Bordetella Moreno-López 1952 Brackiella Willems, Gilhaus, beer, Mietke, Gelderblom, Burghardt, Voigt & Reissbrodt 2002 Candidimonas Vaz-Moreira, Figueira, Lopes, de Brandt, Vandamme, Nunes & Manaia 2011 Castellaniella Kämpfer, Denger, Cook, Lee, Jäckel, Denner & Busse 2006 Derxia Jensen, Petersen, De & Bhattacharya 1960 Kerstersia Coenye, Vancanneyt, Cnokaert, Falsen, Swings & Vandamme 2003 Oligella Rossau, Kersters, Falsen, Jantzen, Segers, Union, Nehls & De Ley 1987 Paenalcaligenes Kämpfer, Falsen, Langer, Lodders & Busse 2010 Paralcaligenes Kim, Yoo, Weon, Kim, Anadham, Suh & Kwon 2011 Parapusillimonas Kim, Kim, Im, Srinivasan & Yang 2010 Pelistega Vandamme, Segers, Ryll, Hommez, Vancanneyt, ...
SUMMARY Steel and Cowan (1964) designated Schaub's Biol. 1 as the type strain for Bacterium anitratum Schaub and Hauber 1948. However, this strain was not included among those originally studied by Schaub and Hauber (1948) and cannot be recognized (Rule 9d, Note b, Intl. Code of Nomenclature of Bacteria). One of the original strains used by Schaub and Hauber (Schaub 81, American Type Culture Collection (ATCC) 19606, RH 2208) is here designated as the type for B. anitratum Schaub and Hauber 1948. The morphology, physiology, and per cent guanine plus cytosine of strain 81 are described and more than sixty characters of the strain recorded. The characteristics of strain 81 were found to be in good agreement with those given in the original description by Schaub and Hauber (1948).
Endogenous bacterial contaminants isolated from infected cultures of Ilex dumosa nodal segments were identified as Stenotrophomonas maltophilia and Achromobacter sp. using 16S rDNA analysis. A range o
JMP Training is pleased to announce a new live web class. JMP Software: Analysis of Attribute Data has been updated for JMP version 7 and has been
Hydrogenases are metalloenzymes that reversibly catalyse the oxidation or production of molecular hydrogen (H2). Amongst a number of promising candidates for application in the oxidation of H2 is a soluble [Ni-Fe] uptake hydrogenase (SH) produced by Cupriavidus necator H16. In the present study, molecular characterisation of the SH operon, responsible for functional SH synthesis, was investigated by developing a green fluorescent protein (GFP) reporter system to characterise PSH promoter activity using several gene cloning approaches. A PSH promoter-gfp fusion was successfully constructed and inducible GFP expression driven by the PSH promoter under de-repressing conditions in heterotrophic growth media was demonstrated in the recombinant C. necator H16 cells. Here we report the first successful fluorescent reporter system to study PSH promoter activity in C. necator H16. The fusion construct allowed for the design of a simple screening assay to evaluate PSH activity. Furthermore, the constructed
The mechanism of the enzymatic degradation of poly([R]-3-hydroxybutyrate) (PHB) was investigated by using well-defined model substrates, including both linear and cyclic [R]-3-hydroxybutyrate (3HB) and [R]-3-hydroxyvalerate (3HV) oligomers, with two different PHB depolymerases. The linear and cyclic oligomers containing from 2 to 10 repeating units were hydrolyzed in solutions of the depolymerase isolated from Aspergillus fumigatus and Alcaligenes faecalis, and the rates of hydrolysis and types of products formed were characterized. Both of the depolymerases catalyzed the hydrolysis of the cyclic oligomers (macrolides) which contained more than three 3HB and 3HV repeating units. The degradation reactions of the linear and cyclic 3HB oligomers with the A. fumigatus depolymerase gave similar ratios of monomer-to-dimer products, but PHB itself formed mostly monomer on hydrolysis, indicating that the enzymatic hydrolysis reactions occurred by different mechanisms for these different types of ...
How is 2,4-Dichlorophenoxyacetic Acid abbreviated? 24-D stands for 2,4-Dichlorophenoxyacetic Acid. 24-D is defined as 2,4-Dichlorophenoxyacetic Acid frequently.
A method for controlling and modifying biopolymer synthesis by manipulation of the genetics and enzymology of synthesis of polyhydroxybutyrate (PHB) and polyhydroxyalkanoate (PHA) polyesters at the molecular level in procaryotic and eukaryotic cells, especially plants. Examples demonstrate the isolation, characterization, and expression of the genes involved in the production of PHB and PHA polymers. Genes encoding the enzymes in the PHB and PHA synthetic pathway (beta-ketothiolase, acetoacetyl-CoA reductase and PHB polymerass or PHA polymerase) from Zoogloea ramigera strain I-16-M, Alcaligenes eutrophus, Nocardia salmonicolur, and P. olevorans were identified or isolated and expressed in a non-PHB producing organism, E. coli. Specific modifications to the polymers include variation in the chain length of the polymers and incorporation of different monomers into the polymers to produce co-polymers with different physical properties.
Ann Arbor, MI: University Microfilms International, 1994. Leiden, The Netherlands: Brill, 2007. Grove, Linda; Daniels, Christian). Tokyo: University of Tokyo Press, 1984. New Haven: Yale University Press, 1984. Ann Arbor: Association for Asian Studies, 1998. TAs rather alone produce( S)-selectivity. TA from Alcaligenes eutrophus( Banerjee et al. TA from Arthrobacter problem by modified feature need for their way Prejudice regional. This download major crops and water scarcity in egypt irrigation water in administrator had video.
filtered_set; syntelog; UniRef90: B6UB13_MAIZE Blue copper protein n=1 (Zea mays) Exp=7e-60; maizesequence.org: RefSeq_peptide:NP_001152236; blue copper protein , UniGene:Zm.136022; Blue copper protein , UniGene:Zm.24862; Blue copper protein , Uniprot/SPTREMBL:B6TFN3; Blue copper protein , Uniprot/SPTREMBL:B6UB13; Blue copper protein , RefSeq_peptide:NP_001149576; blue copper protein , RefSeq_dna:NM_001158764; blue copper protein (LOC100285874) mRNA , RefSeq_dna:NM_001156104; blue copper protein (LOC100283202) mRNA , GO:0009055; electron carrier activity , GO:0005507; copper ion binding , EntrezGene:100285874; blue copper protein , EntrezGene:100283202; blue copper ...
ID D4X7V4_9BURK Unreviewed; 233 AA. AC D4X7V4; DT 15-JUN-2010, integrated into UniProtKB/TrEMBL. DT 15-JUN-2010, sequence version 1. DT 07-JUN-2017, entry version 26. DE SubName: Full=DnaA regulatory inactivator Hda {ECO:0000313,EMBL:EFF77050.1}; GN Name=hda {ECO:0000313,EMBL:EFF77050.1}; GN ORFNames=HMPREF0004_1551 {ECO:0000313,EMBL:EFF77050.1}; OS Achromobacter piechaudii ATCC 43553. OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; OC Alcaligenaceae; Achromobacter. OX NCBI_TaxID=742159 {ECO:0000313,EMBL:EFF77050.1, ECO:0000313,Proteomes:UP000004510}; RN [1] {ECO:0000313,Proteomes:UP000004510} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 43553 {ECO:0000313,Proteomes:UP000004510}; RA Muzny D., Qin X., Deng J., Jiang H., Liu Y., Qu J., Song X.-Z., RA Zhang L., Thornton R., Coyle M., Francisco L., Jackson L., Javaid M., RA Korchina V., Kovar C., Mata R., Mathew T., Ngo R., Nguyen L., RA Nguyen N., Okwuonu G., Ongeri F., Pham C., Simmons D., RA Wilczek-Boney K., ...
Acordas (ACOR) performance in Q3 misses earnings and revenue estimates. However, the companys key drug Ampyra witnessed increase in sales year over year and inched up sequentially.
Bacteria of the Achromobacter genus, more particularly xylosoxidans species, are responsible for various healthcare associated infections (HAI) which are increasingly described since the last decade. Cystic fibrosis (CF) patients are considered as potential reservoirs in hospitals. We performed a retrospective study to estimate the frequencies of Achromobacter spp. HAI among patients from French West Indies, to determine characteristics of infected patients and establish a possible link between CF and infections. All adults with at least one Achromobacter spp. positive sample and infection criteria in accordance with European official definitions of HAI, hospitalized in University Hospital of Martinique from 2006 to 2016 for more than 48 h, were included. Patient clinical features, immune status and underlying diseases were obtained from medical files. A list of CF patients was given by clinicians. Antibiotic-susceptibility profiles of the strains were determined using an automated method. Mean
Le Comamonadaceae sono una famiglia di betaprotobatteri. Come tutti i protobatteri, sono Gram-negativi. Sono aerobici e la maggior parte delle sue specie sono mobili per via dei flagelli e a forma di bastoncello.. ^ Garrity, George M.; Brenner, Don J.; Krieg, Noel R.; Staley, James T. (eds.) (2005). Bergeys Manual of Systematic Bacteriology, Volume Two: The Proteobacteria, Part C: The Alpha-, Beta-, Delta-, and Epsilonproteobacteria. New York, New York: Springer. ISBN 978-0-387-24145-6. Willems A., J. De Ley, M. Gillis, and K. Kersters. Comamonadaceae, a New Family Encompassing the Acidovorans rRNA Complex, Including Variovorax paradoxus gen. nov.,comb. nov. for Alcaligenes paradoxus (Davis 1969). 1991. International Journal of Systematic Bacteriology. 41: 445-450 Altri progetti Wikimedia Commons Wikispecies Wikimedia Commons contiene immagini o altri file su Comamonadaceae Wikispecies contiene informazioni su Comamonadaceae Comamonadaceae J.P. Euzéby: List of Prokaryotic names with Standing ...
This document explains the procedure for screening soils and aqueous matrices to determine whether 2,4-dichlorophenoxyacetic acid (2,4-D) [Chemical Abstracts Service (CAS) Registry 94-75-7] is likely to be present.. You may need Adobe Reader to view files on this page. See EPAs About PDF page to learn more. ...
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The purple sulphur phototrophic bacterium, Thiocapsa roseopersicina BBS, contains several NiFe hydrogenases. One of these enzymes (HynSL) is membrane associated, remarkably stable and can be used for practical applications. HupSL is also located in the photosynthetic membrane, its properties are similar to other known Hup-type NiFe hydrogenases. A third hydrogenase activity was located in the soluble fraction and was analogous to the NAD-reducing hydrogenases of cyanobacteria. The hoxEFUYH genes are transcribed together. HoxE is needed for the in vivo electron flow to and from the soluble hydrogenase. Some of the accessory genes were identified using random mutagenesis, and sequencing of the T. roseopersicina genome is in progress. The HupD, HynD and HoxW gene products corresponded to the proteases processing the C-termini of the three NiFe hydrogenases respectively. HypF and HupK mutants displayed significant in vivo H2 evolution, which could be linked to the nitrogenase activity for the ΔhypF ...
Indiana University, Bloomington, IN 47405. The genes encoding the RNA subunit of ribonuclease P (RNase P RNA) from the a-purple eubacteria Agrobacterium tumefaciens (402nt) and Rhodospirillum rubrum (429nt), the b-purple eubacterium Alcaligenes eutrophus (341nt) and the d-purple eubacterium Desulfovibrio desulfuricans (360nt) have been cloned and sequenced. Taken with the previously sequenced genes for RNase P RNAs from several g-purple, this represents a sampling of each of the phylogenetic branches of purple eubacteria.. These RNase P RNA sequences allow refinement of the phylogenetic model for RNase P RNA secondary structure, which was originally based on the sequences of RNase P RNAs from four species of the genus Bacillus (Gram positive eubacteria) and seven g-purple eubacteria (mostly closely related enterobacteria). The phylogenetic model has been strengthened by covariation of previously invarient nucleotides within helical elements in the structure model. The evidence for all previously ...
Droog, F..; Hooykaas, P..; Van Der Zaal, B..J.., 1995: 2,4-Dichlorophenoxyacetic Acid and Related Chlorinated Compounds Inhibit Two Auxin-Regulated Type-III Tobacco Glutathione S-Transferases
In an effort to engineer plants to be resistant to glyphosate, scientists starting looking for glyphosate resistant EPSP in the 1980s. Several methods were employed including selection, directed evolution, site-directed mutagenesis, and microbial screens. It was difficult to obtain a suitable enzyme because typically the resistant enzymes had an undesirable decrease in catalytic activity. Eventually, naturally occurring glyphosate-tolerant microbes were identified including Agrobacterium sp. Strain CP4, Achromobacter sp. Strain LBAA, and Pseudomonas sp. Strain PG2982. The enzymes isolate from these maintained good catalyctic ativity while being resistant to glyphosate (Funke). Agrobacterium sp. Strain CP4 was obtained from a glyphosate rich waste area at a glyphosate production facility. There is substantial sequence variation between these resistant enzymes and those of plants or E. coli. Other versions resistant versions of EPSP have been identified in Streptococcus pneumonia and ...
Ochrobactrum anthropi is a common soil alphaproteobacterium (7) that can interact with or colonize many eukaryotic organisms, leading to different outcomes, such as disease or even growth of some plants (references 4 and 13 and references therein). O. anthropi, a close relative of brucellae, has not garnered much attention (5, 8) but is becoming increasingly recognized as a potentially problematic opportunistic and nosocomial pathogen (13). A rising number of reported cases includes some potentially life-threatening infections, such as endocarditis (6, 9, 14). These reports, together with the organisms intrinsic multiresistance to antibiotics (13), could lead to a situation resembling that of an Acinetobacter sp., whose importance as a highly problematic nosocomial pathogen skyrocketed in the past few years (12). The increasing relevance of O. anthropi to human health, together with its phylogenetic proximity to the highly pathogenic brucellae (a USDA Select Agent), prompted us to determine the ...
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DI-fusion, le Dépôt institutionnel numérique de lULB, est loutil de référencementde la production scientifique de lULB.Linterface de recherche DI-fusion permet de consulter les publications des chercheurs de lULB et les thèses qui y ont été défendues.
Acor Orthopaedic is a manufacturer of custom and retail orthopedic products and a converter of EVA, PE and PU foam products serving a variety of markets.
In this study, in order to construct a model of leftover bath water, we analyzed one hundred samples of used bath water samples which were provided by twenty-eight volunteer families. It appeared that the number of detected bacteria from such bath water was correlated closely with the number of bathers. Moreover, the pH, acidity, chemical oxygen demand (COD), ion, protein content of the leftover bath water were measured. The number of bathers had no connection with the pH, acidity, COD, and ion content of the leftover bath water. However, the protein content of the bath water correlated with the number of detected bacteria. Based on these results, the model of leftover bath water was constructed. ,I,Achromobacter xylosoxidans, Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa,/I, were incubated with the model bath wateras indices of bath water contamination. The number of incubated viable cells in the model bath water increased ...
The SCOP classification for the Quinohemoprotein amine dehydrogenase A chain, domain 3 superfamily including the families contained in it. Additional information provided includes InterPro annotation (if available), Functional annotation, and SUPERFAMILY links to genome assignments, alignments, domain combinations, taxonomic visualisation and hidden Markov model information.
As explained here, Vitamin C in its pure form (i.e. ascorbic acid) is highly unstable when in contact with air, as it oxidizes rapidly. This packaging innovation allow to protect the powder of Vitamin C from contact with air as long as the packaging is not "activated". Once done, the shelf life of the formula is short (7 days according to Clinique), as Vitamin C will degrade rapidly in the emulsion. Once activated by pressing on the top of the pack, the Vitamin C is released into the emulsion. Shaking the pack homogenize the mixture for later use.. According to the packaging, Vitamin C in this formula is used at a concentration of 10%.. The emulsion itself, according to the INCI list, is actually a dispersion of oils in a gel. The gel is made of Alcasealan (INCI : Alcaligenes Polysaccharides), a natural polysaccharide forming a 3D network that has emulsion stabilizing properties and Xanthan Gum. This gel of polysaccharides contains an oil phase made of dimethicone (linear silicone) and squalane, ...
Boc Sciences is the worlds leading provider for special chemicals. We offer qualified products for 42558-50-9((+)-Methyl (S)-3-hydroxyvalerate),please inquire us for 42558-50-9((+)-Methyl (S)-3-hydroxyvalerate).
Data on 6,500 pesticides, insecticides and herbicides including toxicity, water pollution, ecological toxicity, uses and regulatory status.
The invention includes a sputtering target containing copper of a purity of at least about 99.999 wt. %, and at least one component selected from the group consisting of Ag, Sn, Te, In, B, Bi, Sb, and P dispersed within the copper. The total of Ag, Sn, Te, In, B, Bi, Sb, and P within the copper is from at least 0.3 ppm to about 10 ppm. The sputtering target has a substantially uniform grain size of less than or equal to about 50 micrometers throughout the copper and the at least one component.
If you draw the ellipse with a string fixed at each foci, the length of the string is the major axis, 2a. The minor axis (x=0) is 2b. The ratio of the distance between the two foci to the length of the major axis is e, the eccentricity of the ellipse. So the distance between the two foci is 2ae which means that the foci are located at x=ae and x=-ae ...
Use of Fe3O4 Nanoparticles for Enhancement of Biosensor Response to the Herbicide 2,4-Dichlorophenoxyacetic Acid. . Download books free in pdf. Online library with books, university works and thousands of documents available to read online and download.

Alcaligenes faecalis subsp. faecalis Castellani and Chalmers ATCC ®Alcaligenes faecalis subsp. faecalis Castellani and Chalmers ATCC ®

Alcaligenes faecalis subsp. faecalis ATCC ® 8750™ Designation: 16 TypeStrain=True Application: Produces nitrilase Quality ... Nucleotide (GenBank) : X05518 Alcaligenes faecalis (ATCC 8750) 5S ribosomal RNA. Nucleotide (GenBank) : AF114786 Alcaligenes ... Alcaligenes faecalis subsp. faecalis Castellani and Chalmers (ATCC® 8750™) Strain Designations: 16 [NCIB 8156] / Type Strain: ... Genomic DNA from Alcaligenes faecalis subsp. faecalis strain 16 [ATCC® 8750™] (ATCC® 8750D-5™) Add to ...
more infohttps://www.atcc.org/Products/Collections/Preceptrol_Cultures/8750.aspx

Alcaligenes faecalis subsp. faecalis Castellani and Chalmers ATCC ®Alcaligenes faecalis subsp. faecalis Castellani and Chalmers ATCC ®

Alcaligenes faecalis subsp. faecalis ATCC ® 35655™ Designation: LRA 41 02 82 TypeStrain=False Application: Quality control ... Alcaligenes faecalis subsp. faecalis Castellani and Chalmers (ATCC® 35655™) Strain Designations: LRA 41 02 82 / Type Strain: no ... Alcaligenes faecalis subsp. faecalis Castellani and Chalmers (ATCC® 35655-MINI-PACK™) Add to ...
more infohttps://www.atcc.org/en/Products/Quality_Control_Strains/By_Instrument/35655.aspx?slp=1

Oxygenation and spontaneous deamination of 2-aminobenzenesulphonic acid in Alcaligenes sp. strain O-1 with subsequent meta ring...Oxygenation and spontaneous deamination of 2-aminobenzenesulphonic acid in Alcaligenes sp. strain O-1 with subsequent meta ring...

Oxygenation and spontaneous deamination of 2-aminobenzenesulphonic acid in Alcaligenes sp. strain O-1 with subsequent meta ring ... Oxygenation and spontaneous deamination of 2-aminobenzenesulphonic acid in Alcaligenes sp. strain O-1 with subsequent meta ring ... Oxygenation and spontaneous deamination of 2-aminobenzenesulphonic acid in Alcaligenes sp. strain O-1 with subsequent meta ring ... 2-Aminobenzenesulphonic acid (2AS) is degraded by Alcaligenes sp. strain O-1 via a previously detected but unidentified ...
more infohttp://www.biochemj.org/content/300/2/429

Role of arginine residues of D-aminoacylase from Alcaligenes x...Role of arginine residues of D-aminoacylase from Alcaligenes x...

Role of arginine residues of D-aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6.: To investigate the role of ... from Alcaligenes xylosoxydans subsp. xylosoxydans A-6 (Alcaligenes A-6) were substituted with lysine by site-directed ... Role of arginine residues of D-aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6.. Authors * Yoshimune, ...
more infohttps://www.mysciencework.com/publication/show/role-arginine-residues-d-aminoacylase-from-alcaligenes-xylosoxydans-subsp-xylosoxydans-6-3201f3a2

دانلود مقاله BIODEGRADATION OF PHENOL USING FREE AND IMMOBIIZED ALCALIGENES FAECALISدانلود مقاله BIODEGRADATION OF PHENOL USING FREE AND IMMOBIIZED ALCALIGENES FAECALIS

... سال انتشار: ۱۳۸۸ ... The cell growth and substrate degradation of phenol as single substrates for Alcaligenes faecalis in batch cultures (shaking ... These results indicate that immobilized Alcaligenes faecalis possesses a good application potential in the treatment of phenol- ... Biological degradation of phenol by Alcaligenes faecalis with high biodegradation activity and high tolerance was investigated ...
more infohttp://www.getpaper.ir/biodegradation-of-phenol-using-free-and-immobiized-alcaligenes-faecalis.htm

Achromobacter xylosoxidans | definition of Achromobacter xylosoxidans by Medical dictionaryAchromobacter xylosoxidans | definition of Achromobacter xylosoxidans by Medical dictionary

Alcaligenes xylosoxidans. (redirected from Achromobacter xylosoxidans). Also found in: Encyclopedia. Alcaligenes xylosoxidans. ... Alcaligenes xylosoxidans. A species that does not ferment glucose. It has been implicated rarely in lung infections in patients ... Alcaligenes xylosoxidans. An aerobic, motile, oxidase and catalase positive, nonfermentative gram-negative environmental ... a href=https://medical-dictionary.thefreedictionary.com/Achromobacter+xylosoxidans,Alcaligenes xylosoxidans,/a,. *Facebook ...
more infohttps://medical-dictionary.thefreedictionary.com/Achromobacter+xylosoxidans

Bordetella bronchiseptica (Alcaligenes bronchisepticus)Bordetella bronchiseptica (Alcaligenes bronchisepticus)

Alcaligenes bronchisepticus. Other names i. › "Alcaligenes bronchicanis" (Ferry 1911) Haupt 1935. › "Alcaligenes ... Alcaligenes bronchicanis. › Bacillus bronchicanis. › Bacillus bronchisepticus. › Bacterium bronchisepticus. › Bordetella ...
more infohttp://www.uniprot.org/taxonomy/518

Alcaligenes faecalis subsp. faecalis Castellani and Chalmers ATCC ®Alcaligenes faecalis subsp. faecalis Castellani and Chalmers ATCC ®

Alcaligenes faecalis subsp. faecalis ATCC ® 35655-MINI-PACK™ Designation: LRA 41 02 82 TypeStrain=False Application: ATCC ® ... Alcaligenes faecalis subsp. faecalis Castellani and Chalmers ATCC® 35655-MINI-PACK™ frozen 6 ready-to-use vials of ATCC® 35655 ... Alcaligenes faecalis subsp. faecalis Castellani and Chalmers (ATCC® 35655-MINI-PACK™) Strain Designations: LRA 41 02 82 / Type ...
more infohttp://atcc.org/en/Products/Cells_and_Microorganisms/ATCC_Minis/35655-MINI-PACK.aspx

Alcaligenes | Tabers Medical DictionaryAlcaligenes | Taber's Medical Dictionary

Alcaligenes answers are found in the Tabers Medical Dictionary powered by Unbound Medicine. Available for iPhone, iPad, ... Alcaligenes is a sample topic from the Tabers Medical Dictionary. To view other topics, please sign in or purchase a ... Alcaligenes xylosoxidans. A species that does not ferment glucose. It has been implicated rarely in lung infections in patients ... Alcaligenes faecalis. A species normally found in the human intestine. It has been associated with hospital-acquired septicemia ...
more infohttps://www.tabers.com/tabersonline/view/Tabers-Dictionary/732370/all/Alcaligenes

A novel multicomponent regulatory system mediates H2 sensing in Alcaligenes eutrophus | PNASA novel multicomponent regulatory system mediates H2 sensing in Alcaligenes eutrophus | PNAS

Alcaligenes strains were grown in mineral salts medium (20) containing 0.4% succinate (SN) or 0.2% fructose and 0.2% glycesd ( ... The proteobacterium Alcaligenes eutrophus is one of the best-studied facultative lithoautotrophs and well adapted to the ... A novel multicomponent regulatory system mediates H2 sensing in Alcaligenes eutrophus Message Subject (Your Name) has sent you ... A novel multicomponent regulatory system mediates H2 sensing in Alcaligenes eutrophus. Oliver Lenz and Bärbel Friedrich ...
more infohttps://www.pnas.org/content/95/21/12474?ijkey=a08f7c962b9e86f189a806993d3e96ea481720f2&keytype2=tf_ipsecsha

Alcaligenes xylosoxidans | definition of Alcaligenes xylosoxidans by Medical dictionaryAlcaligenes xylosoxidans | definition of Alcaligenes xylosoxidans by Medical dictionary

What is Alcaligenes xylosoxidans? Meaning of Alcaligenes xylosoxidans medical term. What does Alcaligenes xylosoxidans mean? ... Looking for online definition of Alcaligenes xylosoxidans in the Medical Dictionary? Alcaligenes xylosoxidans explanation free ... Alcaligenes xylosoxidans. Also found in: Encyclopedia. Alcaligenes xylosoxidans. a bacterial species isolated from blood, ... Alcaligenes xylosoxidans. A species that does not ferment glucose. It has been implicated rarely in lung infections in patients ...
more infohttp://medical-dictionary.thefreedictionary.com/Alcaligenes+xylosoxidans

Alcaligenes - WikipediaAlcaligenes - Wikipedia

Alcaligenes is a genus of Gram-negative, aerobic, rod-shaped bacteria. The species are motile with one or more peritrichous ... Strains of Alcaligenes (such as A. faecalis) are found mostly in the intestinal tracts of vertebrates, decaying materials, ... Alcaligenes species have been used for the industrial production of nonstandard amino acids; A. eutrophus also produces the ... Alcaligenes species have been increasingly recovered over the past decade from patients with cystic fibrosis (CF). An ...
more infohttps://en.wikipedia.org/wiki/Alcaligenes

Alcaligenes piechaudii - WikipediaAlcaligenes piechaudii - Wikipedia

Alcaligenes piechaudii is a bacterium; its type strain is CIP 60.75 (= Hugh 366-5 = IAM 12591 = LMG 1873). It is rod-shaped, ... Kiredjian, M.; Holmes, B.; Kersters, K.; Guilvout, I.; De Ley, J. (1986). "Alcaligenes piechaudii, a New Species from Human ... Peel MM, Hibberd AJ, King BM, Williamson HG (August 1988). "Alcaligenes piechaudii from chronic ear discharge". Journal of ...
more infohttps://en.wikipedia.org/wiki/Alcaligenes_piechaudii

SEM of Alcaligenes ruhlandii - Stock Image - B220/0535 - Science Photo LibrarySEM of Alcaligenes ruhlandii - Stock Image - B220/0535 - Science Photo Library

... of a cluster of Alcaligenes ruhlandii, a type of Gram-negative bacterium. A. ruhlandii is one of those species of aerobic ... False-colour scanning electron micrograph (SEM) of a cluster of Alcaligenes ruhlandii, a type of Gram-negative bacterium. A. ...
more infohttps://www.sciencephoto.com/media/11164/view/sem-of-alcaligenes-ruhlandii

Alcaligenes Eutrophus and Its Role in Biodegradable Polymer Production - microbewikiAlcaligenes Eutrophus and Its Role in Biodegradable Polymer Production - microbewiki

Other roles of Alcaligenes eutrophus. Bioremediation Agent. Alcaligenes eutrophus have developed resistance to heavy metal ions ... Synthesis in Alcaligenes eutrophus. In A. eutrophus, the synthesis of PHB involves glucose as a carbon source and three enzymes ... Alcaligenes eutrophus forms PHB as a way of fixing carbon and as a form of intercellular storage [4]. When the bacterium runs ... Alcaligenes eutrophus synthesizes different kind of polymers based on the carbon source it is exposed to in the media. For ...
more infohttps://microbewiki.kenyon.edu/index.php/Alcaligenes_Eutrophus_and_Its_Role_in_Biodegradable_Polymer_Production

dhlC - 2-haloacid dehalogenase, configuration-inverting - Alcaligenes xylosoxydans xylosoxydans - dhlC gene & proteindhlC - 2-haloacid dehalogenase, configuration-inverting - Alcaligenes xylosoxydans xylosoxydans - dhlC gene & protein

sp,Q59168,HDDL_ALCXX 2-haloacid dehalogenase, configuration-inverting OS=Alcaligenes xylosoxydans xylosoxydans OX=85698 GN=dhlC ... Alcaligenes xylosoxydans xylosoxydans (Achromobacter xylosoxidans). ,p>This subsection of the ,a href="http://www.uniprot.org/ ...
more infohttps://www.uniprot.org/uniprot/Q59168

RCSB PDB 









- 1GS6: Crystal structure of M144A mutant of Alcaligenes xylosoxidans Nitrite Reductase Literature Report...RCSB PDB - 1GS6: Crystal structure of M144A mutant of Alcaligenes xylosoxidans Nitrite Reductase Literature Report...

Asp92Asn and His254Phe Mutants of the Nitrite Reductase from Alcaligenes Xylosoxidans Provide Insight Into the Enzyme Mechanism ... Crystal structure of M144A mutant of Alcaligenes xylosoxidans Nitrite Reductase. *DOI: 10.2210/pdb1gs6/pdb ...
more infohttp://www.rcsb.org/pdb/explore/litView.do?structureId=1GS6

RCSB PDB 









- 1GS6: Crystal structure of M144A mutant of Alcaligenes xylosoxidans Nitrite Reductase Methods Report PageRCSB PDB - 1GS6: Crystal structure of M144A mutant of Alcaligenes xylosoxidans Nitrite Reductase Methods Report Page

Asp92Asn and His254Phe Mutants of the Nitrite Reductase from Alcaligenes Xylosoxidans Provide Insight Into the Enzyme Mechanism ...
more infohttp://www.rcsb.org/pdb/explore/materialsAndMethods.do?structureId=1GS6

Further experiments on the potassium uptake by Alcaligenes faecalis | Biochemical JournalFurther experiments on the potassium uptake by Alcaligenes faecalis | Biochemical Journal

Further experiments on the potassium uptake by Alcaligenes faecalis Message Subject (Your Name) has forwarded a page to you ... Further experiments on the potassium uptake by Alcaligenes faecalis. R HEMS, HA KREBS ...
more infohttp://www.biochemj.org/content/82/1/80

Identification of cfxR, an activator gene of autotrophic CO2 fixation in Alcaligenes eutrophus.  - PubMed - NCBIIdentification of cfxR, an activator gene of autotrophic CO2 fixation in Alcaligenes eutrophus. - PubMed - NCBI

Identification of cfxR, an activator gene of autotrophic CO2 fixation in Alcaligenes eutrophus.. Windhövel U1, Bowien B. ... A regulatory gene, cfxR, involved in the carbon dioxide assimilation of Alcaligenes eutrophus H16 has been characterized ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/1779759?dopt=Abstract

Chemically modified surface functional groups of Alcaligenes sp. S-XJ-1 to enhance its demulsifying capability | SpringerLinkChemically modified surface functional groups of Alcaligenes sp. S-XJ-1 to enhance its demulsifying capability | SpringerLink

Liu J, Huang XF, Lu LJ, Xu JC, Wen Y, Yang DH, Zhou Q (2010) Optimization of biodemulsifier production from Alcaligenes sp. S- ... Peng KM, Liu J, Lu LJ, Yin W, Huang XF (2016) Cell surface properties of the demulsifying strain Alcaligenes sp. S-XJ-1 ... Chemically modified surface functional groups of Alcaligenes sp. S-XJ-1 to enhance its demulsifying capability. ... Analysis of biological demulsification process of water-in-oil emulsion by Alcaligenes sp. S-XJ-1. Bioresour Technol 101(21): ...
more infohttps://link.springer.com/article/10.1007%2Fs00253-017-8111-1

Tn5563, a transposon encoding putative mercuric ion transport proteins located on plasmid pRA2 of Pseudomonas alcaligenes.  -...Tn5563, a transposon encoding putative mercuric ion transport proteins located on plasmid pRA2 of Pseudomonas alcaligenes. -...

Tn5563, a transposon encoding putative mercuric ion transport proteins located on plasmid pRA2 of Pseudomonas alcaligenes.. Yeo ... Sequence analysis of pRA2, an endogenous 33-kb plasmid from Pseudomonas alcaligenes NCIB 9867 (strain P25X), revealed the ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/9742696?dopt=Abstract

A novel synthesis of iminodiacetic acid: Biocatalysis by whole Alcaligenes faecalis ZJB-09133 cells from iminodiacetonitrile -...A novel synthesis of iminodiacetic acid: Biocatalysis by whole Alcaligenes faecalis ZJB-09133 cells from iminodiacetonitrile -...

Jin-Feng Zhang, Zhi-Qiang Liu, Yu-Guo Zheng, Improvement of nitrilase production from a newly isolated Alcaligenes faecalis ... A novel synthesis of iminodiacetic acid: Biocatalysis by whole Alcaligenes faecalis ZJB-09133 cells from iminodiacetonitrile. ... A strain with the capability of producing nitrilase, ZJB-09133, was isolated and identified, and later named Alcaligenes ...
more infohttp://onlinelibrary.wiley.com/doi/10.1002/btpr.603/abstract

Analysis of a 24-kilodalton protein associated with the polyhydroxyalkanoic acid granules in Alcaligenes eutrophus. | Journal...Analysis of a 24-kilodalton protein associated with the polyhydroxyalkanoic acid granules in Alcaligenes eutrophus. | Journal...

Analysis of a 24-kilodalton protein associated with the polyhydroxyalkanoic acid granules in Alcaligenes eutrophus.. R ... Analysis of a 24-kilodalton protein associated with the polyhydroxyalkanoic acid granules in Alcaligenes eutrophus. ... Analysis of a 24-kilodalton protein associated with the polyhydroxyalkanoic acid granules in Alcaligenes eutrophus. ... Analysis of a 24-kilodalton protein associated with the polyhydroxyalkanoic acid granules in Alcaligenes eutrophus. ...
more infohttps://jb.asm.org/content/177/9/2425?ijkey=f332163b09b3011de256382ebac8548435c2b3a4&keytype2=tf_ipsecsha

异养硝化菌Alcaligenes faecalis NR氨单加氧酶PCR扩增及异养硝化菌Alcaligenes faecalis NR氨单加氧酶PCR扩增及

The amo gene of Alcaligenes faecalis strain NR was amplified successfully, and 1639 bp length DNA sequence was obtained. This ... 成功扩增粪产碱杆菌Alcaligenes faecalis NR基因组DNA中amo基因序列,得到长度为1639bp的DNA序列。该段序列包含1个完整的开放阅读框(ORF),编码长度为346aa的多肽链。经过BLAST在线比对分析,该序列为AMO蛋白质
more infohttps://www.hanspub.org/journal/PaperInformation.aspx?paperID=20968
  • Taber's Medical Dictionary, Taber's Online, https://www.unboundmedicine.com/medline/view/Tabers-Dictionary/732370/all/Alcaligenes. (tabers.com)
  • To characterize the genes of P. alcaligenes S2 involved in solubilization of lecithin, the EcoR I fragments of the chromosomes from the four mutant strains carrying a single transposon were cloned, and the DNA sequences flanking the Tn5 were determined. (springer.com)
  • Among the many hydrogen-oxidizing bacteria, the Alcaligenes are characterized by their ability in forming two hydrogenases: an NAD-reducing hydrogenase found in the cytoplasm and a membrane-bound hydrogenase . (kenyon.edu)