Peptaibols
A group of peptides characterized by length of 1-2 dozen residues with a high proportion of them being non-proteinogenic, notably alpha-aminoisobutyric acid (Aib) and isovaline, and have a C-terminal amino alcohol and N terminal alkyl group. They are found in FUNGI and some are ANTI-INFECTIVE AGENTS. They form channels or pores in target organisms. The term is a contraction of peptide-Aib-alcohol.
Lipid Bilayers
Ion Channels
Melitten
Ionophores
Chemical agents that increase the permeability of biological or artificial lipid membranes to specific ions. Most ionophores are relatively small organic molecules that act as mobile carriers within membranes or coalesce to form ion permeable channels across membranes. Many are antibiotics, and many act as uncoupling agents by short-circuiting the proton gradient across mitochondrial membranes.
Niclosamide
Phosphatidylcholines
Octanes
Tetrathionic Acid
Membranes, Artificial
Trichoderma
Spin Labels
Electrochemistry
Protein Structure, Secondary
Models, Molecular
Phosphatidylethanolamines
Membrane Potentials
The voltage differences across a membrane. For cellular membranes they are computed by subtracting the voltage measured outside the membrane from the voltage measured inside the membrane. They result from differences of inside versus outside concentration of potassium, sodium, chloride, and other ions across cells' or ORGANELLES membranes. For excitable cells, the resting membrane potentials range between -30 and -100 millivolts. Physical, chemical, or electrical stimuli can make a membrane potential more negative (hyperpolarization), or less negative (depolarization).
Dimyristoylphosphatidylcholine
Membrane Fluidity
Glucuronides
Glycosides of GLUCURONIC ACID formed by the reaction of URIDINE DIPHOSPHATE GLUCURONIC ACID with certain endogenous and exogenous substances. Their formation is important for the detoxification of drugs, steroid excretion and BILIRUBIN metabolism to a more water-soluble compound that can be eliminated in the URINE and BILE.
Glucuronosyltransferase
Amino Acid Sequence
Peptides
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
Solvents
Water
Mathematics
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Circular Dichroism
Hydrogen Bonding
Magnetic Resonance Spectroscopy
X-Ray Diffraction
The scattering of x-rays by matter, especially crystals, with accompanying variation in intensity due to interference effects. Analysis of the crystal structure of materials is performed by passing x-rays through them and registering the diffraction image of the rays (CRYSTALLOGRAPHY, X-RAY). (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Alamethicin
Models, Structural
Protein Conformation
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
Electron Spin Resonance Spectroscopy
A technique applicable to the wide variety of substances which exhibit paramagnetism because of the magnetic moments of unpaired electrons. The spectra are useful for detection and identification, for determination of electron structure, for study of interactions between molecules, and for measurement of nuclear spins and moments. (From McGraw-Hill Encyclopedia of Science and Technology, 7th edition) Electron nuclear double resonance (ENDOR) spectroscopy is a variant of the technique which can give enhanced resolution. Electron spin resonance analysis can now be used in vivo, including imaging applications such as MAGNETIC RESONANCE IMAGING.
Models, Biological
Thermodynamics
A rigorously mathematical analysis of energy relationships (heat, work, temperature, and equilibrium). It describes systems whose states are determined by thermal parameters, such as temperature, in addition to mechanical and electromagnetic parameters. (From Hawley's Condensed Chemical Dictionary, 12th ed)
Liposomes
Computer Simulation
Physicochemical Phenomena
Models, Chemical
Chemistry, Physical
Phospholipids
Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.
Gramicidin
Methanol
Potentiometry
Molecular Probes
Ions
Membrane Lipids
Lipids, predominantly phospholipids, cholesterol and small amounts of glycolipids found in membranes including cellular and intracellular membranes. These lipids may be arranged in bilayers in the membranes with integral proteins between the layers and peripheral proteins attached to the outside. Membrane lipids are required for active transport, several enzymatic activities and membrane formation.
Molecular Structure
Proline
Antimicrobial Cationic Peptides
Small cationic peptides that are an important component, in most species, of early innate and induced defenses against invading microbes. In animals they are found on mucosal surfaces, within phagocytic granules, and on the surface of the body. They are also found in insects and plants. Among others, this group includes the DEFENSINS, protegrins, tachyplesins, and thionins. They displace DIVALENT CATIONS from phosphate groups of MEMBRANE LIPIDS leading to disruption of the membrane.
Permeability
Cell Membrane
Amides
Scattering, Radiation
Ion Channel Gating
The opening and closing of ion channels due to a stimulus. The stimulus can be a change in membrane potential (voltage-gated), drugs or chemical transmitters (ligand-gated), or a mechanical deformation. Gating is thought to involve conformational changes of the ion channel which alters selective permeability.
Spectroscopy, Fourier Transform Infrared
Hydrogen
The first chemical element in the periodic table. It has the atomic symbol H, atomic number 1, and atomic weight [1.00784; 1.00811]. It exists, under normal conditions, as a colorless, odorless, tasteless, diatomic gas. Hydrogen ions are PROTONS. Besides the common H1 isotope, hydrogen exists as the stable isotope DEUTERIUM and the unstable, radioactive isotope TRITIUM.
Microsomes, Liver
Phosphatidylserines
Deuterium
Cell Membrane Permeability
Pressure
Temperature
Nuclear Magnetic Resonance, Biomolecular
Crystallization
Structure-Activity Relationship
Membrane Proteins
Hydrogen-Ion Concentration
Calcium
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
Patch-Clamp Techniques
An electrophysiologic technique for studying cells, cell membranes, and occasionally isolated organelles. All patch-clamp methods rely on a very high-resistance seal between a micropipette and a membrane; the seal is usually attained by gentle suction. The four most common variants include on-cell patch, inside-out patch, outside-out patch, and whole-cell clamp. Patch-clamp methods are commonly used to voltage clamp, that is control the voltage across the membrane and measure current flow, but current-clamp methods, in which the current is controlled and the voltage is measured, are also used.
The role of proline and glycine in determining the backbone flexibility of a channel-forming peptide. (1/201)
Alamethicin is a helical 20-amino acid voltage-gated channel-forming peptide, which is known to exhibit segmental flexibility in solution along its backbone near alpha-methylalanine (MeA)-10 and Gly-11. In an alpha-helical configuration, MeA at position 10 would normally hydrogen-bond with position 14, but the presence of proline at this position prevents the formation of this interhelical hydrogen bond. To determine whether the presence of proline at position 14 contributes to the flexibility of this helix, two analogs of alamethicin were synthesized, one with proline 14 replaced by alanine and another with both proline 14 and glycine 11 replaced by alanine. The C-termini of these peptides were derivatized with a proxyl nitroxide, and paramagnetic enhancements produced by the nitroxide on the Calpha protons were used to estimate r-6 weighted distances between the nitroxide and the backbone protons. When compared to native alamethicin, the analog lacking proline 14 exhibited similar C-terminal to Calpha proton distances, indicating that substitution of proline alone does not alter the flexibility of this helix; however, the subsequent removal of glycine 11 resulted in a significant increase in the averaged distances between the C- and N-termini. Thus, the G-X-X-P motif found in alamethicin appears to be largely responsible for mediating high-amplitude bending motions that have been observed in the central helical domain of alamethicin in methanol. To determine whether these substitutions alter the channel behavior of alamethicin, the macroscopic and single-channel currents produced by these analogs were compared. Although the substitution of the G-X-X-P motif produces channels with altered characteristics, this motif is not essential to achieve voltage-dependent gating or alamethicin-like behavior. (+info)An alamethicin channel in a lipid bilayer: molecular dynamics simulations. (2/201)
We present the results of 2-ns molecular dynamics (MD) simulations of a hexameric bundle of Alm helices in a 1-palmitoyl-2-oleoylphosphatidylcholine bilayer. These simulations explore the dynamic properties of a model of a helix bundle channel in a complete phospholipid bilayer in an aqueous environment. We explore the stability and conformational dynamics of the bundle in a phospholipid bilayer. We also investigate the effect on bundle stability of the ionization state of the ring of Glu18 side chains. If all of the Glu18 side chains are ionised, the bundle is unstable; if none of the Glu18 side chains are ionized, the bundle is stable. pKA calculations suggest that either zero or one ionized Glu18 is present at neutral pH, correlating with the stable form of the helix bundle. The structural and dynamic properties of water in this model channel were examined. As in earlier in vacuo simulations (Breed et al., 1996 .Biophys. J. 70:1643-1661), the dipole moments of water molecules within the pore were aligned antiparallel to the helix dipoles. This contributes to the stability of the helix bundle. (+info)Preferential transport of glutathione versus glutathione disulfide in rat liver microsomal vesicles. (3/201)
A bi-directional, saturable transport of glutathione (GSH) was found in rat liver microsomal vesicles. GSH transport could be inhibited by the anion transport blockers flufenamic acid and 4, 4'-diisothiocyanostilbene-2,2'-disulfonic acid. A part of GSH taken up by the vesicles was metabolized to glutathione disulfide (GSSG) in the lumen. Microsomal membrane was virtually nonpermeable toward GSSG; accordingly, GSSG generated in the microsomal lumen could hardly exit. Therefore, GSH transport, contrary to previous assumptions, is preferred in the endoplasmic reticulum, and GSSG entrapped and accumulated in the lumen creates the oxidized state of its redox buffer. (+info)Surface binding of alamethicin stabilizes its helical structure: molecular dynamics simulations. (4/201)
Alamethicin is an amphipathic alpha-helical peptide that forms ion channels. An early event in channel formation is believed to be the binding of alamethicin to the surface of a lipid bilayer. Molecular dynamics simulations are used to compare the structural and dynamic properties of alamethicin in water and alamethicin bound to the surface of a phosphatidylcholine bilayer. The bilayer surface simulation corresponded to a loosely bound alamethicin molecule that interacted with lipid headgroups but did not penetrate the hydrophobic core of the bilayer. Both simulations started with the peptide molecule in an alpha-helical conformation and lasted 2 ns. In water, the helix started to unfold after approximately 300 ps and by the end of the simulation only the N-terminal region of the peptide remained alpha-helical and the molecule had collapsed into a more compact form. At the surface of the bilayer, loss of helicity was restricted to the C-terminal third of the molecule and the rod-shaped structure of the peptide was retained. In the surface simulation about 10% of the peptide/water H-bonds were replaced by peptide/lipid H-bonds. These simulations suggest that some degree of stabilization of an amphipathic alpha-helix occurs at a bilayer surface even without interactions between hydrophobic side chains and the acyl chain core of the bilayer. (+info)C-terminally shortened alamethicin on templates: influence of the linkers on conductances. (5/201)
In order to test the influence of chemical modifications designed to allow covalent coupling of channel-forming peptide motifs into variable sized oligomers, a series of alamethicin derivatives was prepared. The building block encompassing the N-terminal 1-17 residues of alamethicin behaved normally in the conductance assay on planar lipid bilayers, albeit at higher concentration and with a slightly reduced voltage-dependence. A linker Ac-K-OCH(2)C(6)H(4)CH(3)p attached via the epsilon amino group of lysine to the C-terminus of alamethicin(1-17) increased membrane affinity. The latter was further enhanced in a dimer and a tetramer in which alamethicin(1-17) chains were tethered to di- or tetra-lysine linkers, respectively, but macroscopic current-voltage curves displayed much reduced voltage-dependencies and reversed hysteresis. An usual behaviour with high voltage-dependence was restored with the modified dimer of alamethicin(1-17) in which alanine separated the two consecutive lysine residues in the linker. Of special interest was the development of a 'negative resistance' branch in macroscopic current-voltage curves for low concentrations of this dimer with the more flexible linker. Single channel events displayed only one single open state with fast kinetics and whose conductance matches that of the alamethicin heptamer or octamer. (+info)A yeast Golgi E-type ATPase with an unusual membrane topology. (6/201)
E-type ATPases are involved in many biological processes such as modulation of neural cell activity, prevention of intravascular thrombosis, and protein glycosylation. In this study, we show that a gene of Saccharomyces cerevisiae, identified by similarity to that of animal ectoapyrase CD39, codes for a new member of the E-type ATPase family (Apy1p). Overexpression of Apy1p in yeast cells causes an increase in intracellular membrane-bound nucleoside di- and triphosphate hydrolase activity. The activity is highest with ADP as substrate and is stimulated similarly by Ca (2+), Mg(2+), and Mn(2+). The results also indicate that Apy1p is an integral membrane protein located predominantly in the Golgi compartment. Sequence analysis reveals that Apy1p contains one large NH(2)-terminal hydrophilic apyrase domain, one COOH-terminal hydrophilic domain, and two hydrophobic stretches in the central region of the polypeptide. Although no signal sequence is found at the NH(2)-terminal portion of the protein and no NH(2)-terminal cleavage of the protein is observed, demonstrated by the detection of NH(2)-terminal tagged Apy1p, the NH(2)-terminal domain of Apylp is on the luminal side of the Golgi apparatus, and the COOH-terminal hydrophilic domain binds to the cytoplasmic face of the Golgi membrane. The second hydrophobic stretch of Apy1p is the transmembrane domain. These results indicate that Apylp is a type III transmembrane protein; however, the size of the Apy1p extracytoplasmic NH(2) terminus is much larger than those of other type III transmembrane proteins, suggesting that a novel translocation mechanism is utilized. (+info)The ion-channel activity of longibrachins LGA I and LGB II: effects of pro-2/Ala and gln-18/Glu substitutions on the alamethicin voltage-gated membrane channels. (7/201)
Longibrachins LGA I (Ac Aib Ala Aib Ala Aib(5) Ala Gln Aib Val Aib(10) Gly Leu Aib Pro Val(15) Aib Aib Gln Gln Pheol(20), with Aib: alpha-aminoisobutyric acid, pheol: phenylalaninol) and LGB II are two homologous 20-residue long-sequence peptaibols isolated from the fungus Trichoderma longibrachiatum that differ between them by a Gln-18/Glu substitution. They distinguish from alamethicin by a Pro-2 for Ala replacement, which allowed to examine for the first time with natural Aib-containing analogues, the effect of Pro-2 on the ion-channel properties exhibited by alamethicin. The influence of these structural modifications on the voltage-gated ion-channel forming activity of the peptides in planar lipid bilayers were analysed. The general 'barrel-stave' model of ion-channel activity, already described for alamethicin, was preserved with both longibrachins. The negatively charged LGB II promoted higher oligomerisation levels, which could presumably dilute the repulsive effect of the negative Glu ring near the entrance of the channel and resulted in lower lifetimes of the substates, confirming the strong anchor of the peptide C-terminus at the cis-interface. Reduction of the channel lifetimes was observed for the longibrachins, compared to alamethicin. This argues for a better stabilisation of the channels formed by peptaibols having a proline at position 2, which results in better anchoring of the peptide monomer N-terminus at the trans-bilayer interface. Qualitative assays of the temperature dependence on the neutral longibrachin channel properties demonstrated a high increase of channel lifetimes and a markedly reduced voltage-sensitivity when the temperature was decreased, showing that such conditions may allow to study the channel-forming properties of peptides leading to fast current fluctuations. (+info)Voltage-dependent interaction of the peptaibol antibiotic zervamicin II with phospholipid vesicles. (8/201)
The effect of a transmembrane potential on ion channel formation by zervamicin II (ZER-II) was studied in a vesicular model system. The dissipation of diffusion potential caused by addition of ZER-II to small phosphatidylcholine vesicles was monitored using fluorescent (Safranine T) and optical (Oxonol YI) probes. Cis-positive potentials facilitated channel formation, while at cis-negative potentials, ion fluxes were inhibited. A potential-independent behavior of ZER-II was observed at high peptide concentrations, most likely due to its membrane modifying property. (+info)
Structural and biophysical properties of a synthetic channel-forming peptide: designing a clinically relevant anion selective...
Ion channels of N-terminally linked alamethicin dimers: enhancement of cation-selectivity by substitution of Glu for Gln at...
The design, synthesis and characterisation of channel-forming peptides
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Refined structure of peptaibol zervamicin IIB in methanol solution from trans-hydrogen bond J couplings - [scite report]
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Peptaibols
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Peptaibol
The most widely known peptaibol is alamethicin. Chugh JK, Wallace BA (August 2001). "Peptaibols: models for ion channels". ...
Synthetic ion channels
Gramicidin and alamethicin had been popular starting points for selective modifications. The above diagram illustrates one ... The latter, demonstrated in natural channels such as alamethicin, is rarely encountered in synthetic ion channels. They may be ... "Alamethicin−Leucine Zipper Hybrid Peptide: A Prototype for the Design of Artificial Receptors and Ion Channels". Journal of the ...
Orientations of Proteins in Membranes database
Marsh, Derek; Jost, Micha; Peggion, Cristina; Toniolo, Claudio (2007). "TOAC spin labels in the backbone of alamethicin: EPR ...
Amino acid
Both of these amino acids are found in peptidic lantibiotics such as alamethicin. However, in plants, 1-aminocyclopropane-1- ...
Fungal isolate
Penicillin, cephalosporins, fusafungine, usnic acid, fusidic acid, fumagillin, brefeldin A, verrucarin A, alamethicin, are ...
Frederic M. Richards
Fox, R.O.; Richards, F.M. (1982). "A voltage-gated ion channel model inferred from the crystal structure of alamethicin at 1.5- ... the ion-channel-forming alamethicin (PDB: 1AMT) (Fox & Richards 1982), and mutants of ribonuclease S (e.g., PDB: 1RBD;PDB: ...
2-Aminoisobutyric acid
It is rare in nature only being found in meteorites, and some antibiotics of fungal origin, such as alamethicin and some ...
Liosta Bithmhóilíní - Vicipéid
Toisc go bhfuil an liosta bunaithe ar an leagan Béarla i 'Wikipedia en' beidh an leagan Béarla ar dtús agus an leagan Gaeilge ina dhiaidh. Tá súil agam, nuair atá méid áirithe den liosta aistrithe go Gaeilge gur féidir liosta i nGaeilge a chumadh nó i nGaeilge agus i mBéarla. ...
Medicinal fungi
Subsequent discoveries included alamethicin, aphidicolin, brefeldin A, cephalosporin, cerulenin, citromycin, eupenifeldin, ...
Fermentek
Anisomycin, Thiolutin, Wortmannin, K252a, Staurosporine, K252C, Bafilomycin, Alamethicin, Leptomycin, A23187, Chelerythrine, ...
Transporter Classification Database
Family 1.D.3 The Channel-Forming Syringopeptin Family 1.D.4 The Tolaasin Channel-forming Family 1.D.5 The Alamethicin or ...
List of biomolecules
Aequorin Aflatoxin Agar Alamethicin Alanine Albumins Aldosterone Aleurone Alpha-amanitin Alpha-MSH (Melaninocyte stimulating ...
List of MeSH codes (D04)
... alamethicin MeSH D04.345.566.050 - amanitins MeSH D04.345.566.075 - bacitracin MeSH D04.345.566.142 - capreomycin sulfate MeSH ...
List of MeSH codes (D12.644)
... alamethicin MeSH D12.644.641.050 - amanitins MeSH D12.644.641.075 - bacitracin MeSH D12.644.641.142 - capreomycin sulfate MeSH ...
Orientations of Proteins in Membranes database
"TOAC spin labels in the backbone of alamethicin: EPR studies in lipid membranes". Biophys. J. 92 (2): 473-481. doi:10.1529/ ...
Orientations of Proteins in Membranes, a enciclopedia libre
"TOAC spin labels in the backbone of alamethicin: EPR studies in lipid membranes". Biophys. J. 92 (2): 473-481. PMC 1751395 ...
Potential-dependent conductances in lipid membranes containing alamethicin | Philosophical Transactions of the Royal Society B:...
Potential-dependent conductances in lipid membranes containing alamethicin Message Subject (Your Name) has sent you a message ... From the properties of lipid membranes containing alamethicin in a wide variety of electrolytes, and from other evidence, it is ... Potential-dependent conductances in lipid membranes containing alamethicin. L. G. M. Gordon, D. A. Haydon ... that alamethicin forms transient pores of some 0.6 nm in diameter and that, for small inorganic ions, these are poorly ...
Interaction of a helical peptide with membrane: Study of alamethicin
Depending on the alamethicin concentration and the water content in the membrane, alamethicin either perpendicularly inserts ... Our alamethicin-lipid interaction model explains the spontaneous insertion of alamethicin at high aqueous concentration causing ... alamethicin undergoes a transition from the surface state to the insertion state. The insertion of alamethicin would introduce ... Alamethicin is a transmembrane ion channel at low concentration, and a lytic agent of cell membrane at high concentration. It ...
AVHANDLINGAR.SE: Trichoderma viride alamethicin and plant cells - Action, response and resistance
Avhandling: Trichoderma viride alamethicin and plant cells - Action, response and resistance. ... The alamethicin pores allow passage of metabolites and ions and alamethicin can thus be used as a molecular tool for in situ ... In this thesis alamethicin was used for in situ investigations on Nicotiana tabacum BY-2 and Arabidopsis thaliana cell ... Trichoderma viride alamethicin and plant cells - Action, response and resistance. Detta är en avhandling från Media-Tryck ...
Effect of α-helical peptides on liposome structure : A comparative study of melittin and alamethicin
... rupture and fuse in response to the addition of both melittin and alamethicin. In the case of alamethicin, but not melittin, ... Effect of α-helical peptides on liposome structure: A comparative study of melittin and alamethicin. Wessman, Per Uppsala ... By means of a competitive binding assay we furthermore show that alamethicin, in line with earlier findings for melittin, ... Melittin, Alamethicin, Magainin, Liposomes, Affinity, Cryo-transmission electron microscopy National Category Physical ...
Voltage-Dependent Pore Formation and Antimicrobial Activity by Alamethicin and Analogues, The Journal of Membrane Biology | 10...
"Voltage-Dependent Pore Formation and Antimicrobial Activity by Alamethicin and Analogues, The Journal of Membrane Biology" on ... Voltage-Dependent Pore Formation and Antimicrobial Activity by Alamethicin and Analogues. Duclohier, H.; Wróblewski, H. ... Voltage-Dependent Pore Formation and Antimicrobial Activity by Alamethicin and Analogues Duclohier, H. ; Wróblewski, H. 2001-11 ... The demonstration of ar- alamethicin [33] was a landmark, from which new struc- tificial membrane excitability developed by ...
Analysis and evaluation of channel models: simulations of alamethicin. - Oxford Neuroscience
In a 20-ns simulation of a hexameric alamethicin bundle, the main motions are those of individual helices, not of the bundle as ... We have used extended molecular dynamics simulations of alamethicin bundles consisting of 4, 5, 6, 7, and 8 helices in a ... shows that water orientation inside the alamethicin channels is sensitive to the algorithms used. In all cases, water ordering ... Alamethicin is an antimicrobial peptide that forms stable channels with well-defined conductance levels. ...
gmx-users] alamethicin error
Ion channels of N-terminally linked alamethicin dimers: enhancement of cation-selectivity by substitution of Glu for Gln at...
Alamethicin forms voltage-gated ion channels that have moderate cation-selectivity. The enhancement of the cation-selectivity ... by introducing negatively charged residues at positions 7 and 18 has been studied using the tethered homodimers of alamethicin ... Alamethicin forms voltage-gated ion channels that have moderate cation-selectivity. The enhancement of the cation-selectivity ... Ion channels of N-terminally linked alamethicin dimers: enhancement of cation-selectivity by substitution of Glu for Gln at ...
Voltage-dependent insertion of alamethicin at phospholipid/water and octane/water interfaces. - Oxford Neuroscience
Insertion of alamethicin occurs in two steps, corresponding to desolvation of the Gln7 side chain, and the backbone of Aib10 ... Polyalanine and alamethicin form stable helices both when inserted in octane and at the water/octane interface, where they ... We present a detailed analysis of the insertion of alamethicin into the octane slab and the influence of the external field on ... At cis-positive potentials, alamethicin does not insert into a phospholipid bilayer in 10 ns of simulation, due to the slow ...
Products in LKT Labs on Thomas Scientific
Study of polymer molecules and conformations with a nanopore (Patent) | DOepatents
Alamethicin self-assembling in lipid membranes: Concentration dependence from pulsed EPR of spin labels<...
Alamethicin self-assembling in lipid membranes: Concentration dependence from pulsed EPR of spin labels. Physical Chemistry ... Syryamina, V. N., De Zotti, M., Toniolo, C., Formaggio, F., & Dzuba, S. A. (2018). Alamethicin self-assembling in lipid ... Alamethicin self-assembling in lipid membranes : Concentration dependence from pulsed EPR of spin labels. / Syryamina, Victoria ... Syryamina, VN, De Zotti, M, Toniolo, C, Formaggio, F & Dzuba, SA 2018, Alamethicin self-assembling in lipid membranes: ...
"Glossary of Pharmacology Stubs" related terms, short phrases and...
Alamethicin. *Alamethicin is a peptide.. *Alamethicin is a well-studied channel-forming peptide that has a prototypical ... ALAMETHICIN. ALBENDAZOLE. ALFUZOSIN. ALKYLATING AGENT. ALLOPURINOL. ALPHA HYDROXY ACID. ALPHA BLOCKER. AMIDRINE. AMILORIDE. ... In this model alamethicin helices associate to form a bundle with a central lumen, like a barrel made of helical peptides as ...
Determining the Structure and Orientation of the Alamethicin Ion Channel in Lipid Membranes using ATR and Electrochemical...
Alamethicin is a short antibiotic peptide produced by the fungus Trichoderma viride. Ion channels formed by Alamethicin are ... In order to determine the opening and closing of the Alamethicin pore, a bilayer containing Alamethicin was deposited onto the ... The goals of this project were to monitor the incorporation of Alamethicin into 1,2-diphytanoyl-sn-glycero-3-phosphocholine ( ... Determining the Structure and Orientation of the Alamethicin Ion Channel in Lipid Membranes using ATR and Electrochemical ...
Open Physics
Advances in Biological Solid-State NMR (RSC Publishing)
Infrared Spectroscopy as a Probe for the Development of Secondary Structure in the Amino-Terminal Segment of Alamethicin...
Infrared Spectroscopy as a Probe for the Development of Secondary Structure in the Amino-Terminal Segment of Alamethicin ... Interest in the stereochemistry of Aib containing peptides, stems from the fact that alamethicin [7,8] and the related ... Here, we report the results of an infrared spectroscopic study of synthetic alamethicin fragments and demonstrate the ... Infrared Spectroscopy as a Probe for the Development of Secondary Structure in the Amino-Terminal Segment of Alamethicin. In: ...
Molecular structure of t-butyloxycarbonyl-Leu-Aib-Pro-Val-Aib-methyl ester, a fragment of alamethicin and suzukacillin: A 310...
The pentapeptide Boc-Leu-Aib-Pro-Val-Aib-OMe, a fragment of alamethicin and suzukacillin, crystallizes in the space group P21, ... Molecular structure of t-butyloxycarbonyl-Leu-Aib-Pro-Val-Aib-methyl ester, a fragment of alamethicin and suzukacillin: A 310- ... a fragment of alamethicin and suzukacillin: A 310-helical pentapeptide Biopolymers, 21 (12). pp. 2461-2472. ISSN 0006-3525 ...
Effects of membrane channel-forming polypeptides on mitochondrial oxidative phosphorylation. A comparison of alamethicin,...
A comparison of alamethicin, gramicidin A, melittin and tetraacetyl melittin. (deposited 20 May 2010 06:48) * Effects of ... A comparison of alamethicin, gramicidin A, melittin and tetraacetyl melittin. (deposited 20 May 2010 05:02) [Currently ... A comparison of alamethicin, gramicidin A, melittin and tetraacetyl melittin. In: Biochemistry International, 11 (3). 357 -363 ... At low Pi (2.5 mM) the order of uncoupling efficiencies is gramicidin A much greater than alamethicin greater than tetraacetyl ...
Cell Biology | A.G. Scientific, Inc. | Cell biology trends
Siegert M[au] - PubMed - NCBI
Peptide Bond Formation in Non-ribosomal Systems | SpringerLink
Martin, D.R., Williams, J.P.: Chemical nature and sequence of alamethicin. Biochem. J. 153, 181-190 (1976)PubMedGoogle Scholar ... Rindfleisch, H., Kleinkauf, H.: Biosynthesis of alamethicin. FEBS Lett. 62, 276-280 (1976)PubMedGoogle Scholar ... Kleinkauf, H., Rindfleisch, H.: Non-ribosomal biosynthesis of the cyclic octadecapeptide alamethicin. Acta microbiol. Acad. Sci ...
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Correlating kinetic and structural data on ubiquinone binding and reduction by respiratory complex I | PNAS
... the omission of alamethicin. Alamethicin was used previously (23) to open pores in the membrane to allow NADH to access all of ... Alamethicin has been reported to sequester membrane-bound fatty acids (30) so it may sequester quinones also, and to induce ... 2017) Alamethicin can capture lipid-like molecules in the membrane. J Chem Phys 146:011103. ... 2014) Antimicrobial peptide alamethicin insertion into lipid bilayer: A QCM-D exploration. Colloids Surf B Biointerfaces 116: ...
Stankowski S[au] - PubMed - NCBI
Voltage-dependent pore activity of the peptide alamethicin correlated with incorporation in the membrane: salt and cholesterol ... Thermodynamic analysis of incorporation and aggregation in a membrane: application to the pore-forming peptide alamethicin. ... Incorporation kinetics in a membrane, studied with the pore-forming peptide alamethicin. ...
Multi-parameter Measurement of the Permeability Transition Pore Opening in Isolated Mouse Heart Mitochondria | Protocol ...
Multi-parameter Measurement of the Permeability Transition Pore Opening in Isolated Mouse Heart Mitochondria | Protocol ...
Peptaibol - Wikipedia
à la | definition of à la by Medical dictionary
Peptide antibiotic alamethicinBilayerAntibioticPeptidesTrichodermaVoltage-gatedMembranes containingPlanar lipid bilayersInsertion of alamethicinProperties of alamethicinMelittinHydrophobicMoleculesCellulase-inducedPoresPore formationTransmembranePermeabilizationUDPGADifferent peptideDPhPCOrientationChannelsInteractionsMolecularVesiclesArabidopsisChannelHelicesInteractionResistanceFormation
Peptide antibiotic alamethicin3
- The antimicrobial action of the peptide antibiotic alamethicin (Alm) is commonly related to peptide self-assembling resulting in the formation of voltage-dependent channels in bacterial membranes, which induces ion permeation. (nsu.ru)
- The natural alpha-helical peptide antibiotic alamethicin forms voltage-gated ion channels in lipid membranes. (lifl.fr)
- Interaction of the peptide antibiotic alamethicin with bilayer- and non-bilayer-forming lipids: Influence of increasing alamethicin concentration on the lipids supramolecular structures. (mpg.de)
Bilayer12
- By the method of oriented circular dichroism (OCD), we are able to determine the orientation of the alamethicin with respect to the membrane bilayer. (rice.edu)
- Depending on the alamethicin concentration and the water content in the membrane, alamethicin either perpendicularly inserts into the bilayer or binds parallel to the membrane surface. (rice.edu)
- By the method of lamellar x-ray diffraction, we found the membrane bilayer thickness reduced by increasing concentration of alamethicin and decreasing relative humidity. (rice.edu)
- When alamethicin concentration is low, the peptide molecules adsorb near the area of lipid head group, which effectively expands the average cross sectional area of the lipid molecules and makes the hydrocarbon chains more disordered, so that the lipid bilayer becomes thinner than pure lipid membrane. (rice.edu)
- We have used extended molecular dynamics simulations of alamethicin bundles consisting of 4, 5, 6, 7, and 8 helices in a palmitoyl-oleolyl-phosphatidylcholine bilayer to evaluate and analyze channel models and to link the models to the experimentally measured conductance levels. (ox.ac.uk)
- At cis-positive potentials, alamethicin does not insert into a phospholipid bilayer in 10 ns of simulation, due to the slow dynamics of the peptide and lipids. (ox.ac.uk)
- In order to determine the opening and closing of the Alamethicin pore, a bilayer containing Alamethicin was deposited onto the surface of a thioglucose modified Au(111) electrode. (uoguelph.ca)
- For two AMPs, alamethicin and novicidin, we observe that the majority of the lipids remain in a planar bilayer conformation but that a number of lipids are involved in the peptide anchoring.These lipids display reduced dynamics.Our study supports previous studies showing that alamethicin adopts a transmembrane arrangement without significant disturbance of the surrounding lipids, while novicidin forms toroidal pores at high concentrations leading to more extensive membrane disturbance. (nih.gov)
- For two AMPs, alamethicin and novicidin, we observe that the majority of the lipids remain in a planar bilayer conformation but that a number of lipids are involved in the peptide anchoring. (nih.gov)
- The bilayer incorporates bundles of alamethicin molecules (purple) that form pore channels in the membrane. (innovations-report.com)
- Neutron in‐plane scattering intensity of alamethicin pores in DLPC bilayer at P / L =1/10 hydrated with D 2 O (data +) or with H 2 O (data o). (els.net)
- Scientists at Lawrence Livermore National Laboratory took an important step in creating a nanobioelectronic device when they combined a silicon nanowire (shown in the picture in gray ), covered it with a lipid bilayer (blue), and incorporated bundles of alamethicin molecules (purple) to form pore channels in the membrane. (medgadget.com)
Antibiotic7
- This article is concerned primarily with the mechanism of the potential-dependent conductance induced in artificial lipid membranes by the cyclic polypeptide antibiotic alamethicin. (royalsocietypublishing.org)
- Alamethicin is a short antibiotic peptide produced by the fungus Trichoderma viride. (uoguelph.ca)
- Here, we report the results of an infrared spectroscopic study of synthetic alamethicin fragments and demonstrate the development of a $3_{10}$ helical structure at the amino-terminus of the antibiotic. (iisc.ac.in)
- The activity of rotenone-sensitive NADH:O 2 oxidoreductase, reflecting the overall activity of the respiratory chain, was measured in a mitochondrial fraction by a novel method based on providing access for NADH to intact mitochondria via alamethicin, a channel-forming antibiotic. (diabetesjournals.org)
- To permit assessment of the electron transport activity despite impermeability of the inner mitochondrial membrane for NADH, the antibiotic alamethicin was used. (diabetesjournals.org)
- Alamethicin is a channel-forming antibiotic known to increase the permeability of biological membranes through the creation of transmembrane pores up to 20 A in diameter, providing ready access for NADH to enter intact mitochondria, as previously demonstrated with rat skeletal muscle homogenate ( 10 ) and rat heart mitochondria ( 11 ). (diabetesjournals.org)
- The antibiotic peptaibol alamethicin from Trichoderma permeabilises Arabidopsis root apical meristem and epidermis but is antagonised by cellulase-induced resistance to alamethicin. (mpg.de)
Peptides3
- Initially, we show that the antimicrobial peptides melittin, alamethicin and magainin 2, in spite of their very different physico-chemical properties and suggested modes of action on membranes, all have high affinity to lipodisks. (diva-portal.org)
- Observations made with these peptides are distinct as seen from data obtained with the hydrophobic peptide alamethicin. (mdpi.com)
- Structure and functions of channel-forming peptides: magainins, cecropins, melittin and alamethicin. (tcdb.org)
Trichoderma2
- Alamethicin is a membrane active peptide secreted from the soil-living fungus Trichoderma viride. (avhandlingar.se)
- Alamethicin is a peptabiol peptide initially produced by species of Trichoderma. (thomassci.com)
Voltage-gated2
- Alamethicin forms voltage-gated ion channels that have moderate cation-selectivity. (semanticscholar.org)
- Ion channels formed by Alamethicin are voltage-gated due to their large molecular dipoles. (uoguelph.ca)
Membranes containing1
- From the properties of lipid membranes containing alamethicin in a wide variety of electrolytes, and from other evidence, it is concluded that the polypeptide reacts to the electric field more probably because it has a large dipole moment than because it binds ions. (royalsocietypublishing.org)
Planar lipid bilayers2
- Alamethicin remains the prototype of this class and its biophysical properties (mostly focussing on channel- or pore-formation in planar lipid bilayers) were and are still intensively studied. (eurekaselect.com)
- Klinke, N., Goldermann, M. and Hanke, W.: The properties of alamethicin incorporated into planar lipid bilayers under the influence of microgravity. (uni-bremen.de)
Insertion of alamethicin6
- When alamethicin concentration reaches the critical point, where membrane structure becomes favorable for the insertion of alamethicin, alamethicin undergoes a transition from the surface state to the insertion state. (rice.edu)
- The insertion of alamethicin would introduce much water into membrane, so the transition would happen only when relative humidity is high. (rice.edu)
- Our alamethicin-lipid interaction model explains the spontaneous insertion of alamethicin at high aqueous concentration causing the lysis of membrane. (rice.edu)
- Voltage-dependent insertion of alamethicin at phospholipid/water and octane/water interfaces. (ox.ac.uk)
- Insertion of alamethicin occurs in two steps, corresponding to desolvation of the Gln7 side chain, and the backbone of Aib10 and Gly11. (ox.ac.uk)
- We present a detailed analysis of the insertion of alamethicin into the octane slab and the influence of the external field on the peptide structure. (ox.ac.uk)
Properties of alamethicin1
- I could also induce resistance against the pore-forming properties of alamethicin in BY-2 cells by incubating cells with the cell wall degrading enzyme cellulase. (avhandlingar.se)
Melittin8
- Cryo-transmission electron microscopy was used in combination with turbidity and leakage measurements to explore and compare the membrane perturbing effects of melittin and alamethicin on POPC-based liposomes of varying composition. (diva-portal.org)
- cholesterol change their shape, rupture and fuse in response to the addition of both melittin and alamethicin. (diva-portal.org)
- In the case of alamethicin, but not melittin, fusion is effectively prevented by inclusion of 10 mol. (diva-portal.org)
- By means of a competitive binding assay we furthermore show that alamethicin, in line with earlier findings for melittin, possess high affinity for positively curved lipid surfaces. (diva-portal.org)
- A comparison of alamethicin, gramicidin A, melittin and tetraacetyl melittin. (iisc.ac.in)
- At low Pi (2.5 mM) the order of uncoupling efficiencies is gramicidin A much greater than alamethicin greater than tetraacetyl melittin greater than melittin. (iisc.ac.in)
- At Pi = 100 mM inhibitory effects are observed for alamethicin and tetraacetyl melittin. (iisc.ac.in)
- Data of six different peptide/lipid systems are shown: alamethicin in diphytanoyl phosphatidylcholine ( DPhPC ), alamethicin in a mixture of dioleoyl phosphatidylcholine ( DOPC ) and dioleoyl phosphatidylethanolamine ( DOPE ), melittin in DPhPC , melittin in DOPC , melittin in palmitoyl‐oleoyl phosphatidylcholine ( POPC ), and melittin in dieicosenoyl phosphatidylcholine (DiC20:1PC). (els.net)
Hydrophobic2
- In the context of the molecular shape concept, the properties of lipopeptides, including surfactins, are shortly presented, and comparisons with the hydrophobic alamethicin sequence are made. (springer.com)
- The absence of response in yeast mitochondria was not simply due to structural limitations, since large-amplitude swelling occurred in the presence of alamethicin, a hydrophobic, helical peptide, forming voltage-sensitive ion channels in lipid membranes. (sigmaaldrich.com)
Molecules1
- As a specific example from Keller's work, alamethicin molecules form ion channels in membranes. (asbmb.org)
Cellulase-induced1
- Also, the cellulase-induced signalling leading to alamethicin resistance needs to be investigated. (avhandlingar.se)
Pores9
- It has already been shown from studies of the fluctuations that can be detected in very small membrane currents, that alamethicin forms transient pores of some 0.6 nm in diameter and that, for small inorganic ions, these are poorly selective. (royalsocietypublishing.org)
- Alamethicin can make pores across membranes with a negative membrane potential, such as the plant plasma membrane. (avhandlingar.se)
- The alamethicin pores allow passage of metabolites and ions and alamethicin can thus be used as a molecular tool for in situ enzyme measurements. (avhandlingar.se)
- Alamethicin is an antimicrobial peptide (AMP) that disturbs the lipid layer of the cell membrane, aggregating and forming pores and causing cell lysis. (thomassci.com)
- This thesis reports the studies of the structure and mechanism of peptide-induced membrane pores by antimicrobial peptide alamethicin and by a peptide named Baxalpha5, which is derived from Bax protein. (rice.edu)
- Our study supports previous studies showing that alamethicin adopts a transmembrane arrangement without significant disturbance of the surrounding lipids, while novicidin forms toroidal pores at high concentrations leading to more extensive membrane disturbance. (nih.gov)
- Alamethicin forms barrel-stave channels without significant perturbation of the lipid (d), while novicidin creates toroidal pores in the lipids (e). (nih.gov)
- Alamethicin adopts transmembrane conformations (Fig. 6d) and novicidin uses the lipids to form toroidal pores with the lipid (Fig. 6e). (nih.gov)
- He K, Ludtke SJ, Worcester DL and Huang HW (1996) Neutron scattering in the plane of membrane: structure of alamethicin pores. (els.net)
Pore formation2
- The molecular conditions that affects alamethicin membrane association and pore formation, in plasma membranes in vivo, remains to be characterised. (avhandlingar.se)
- Voltage-Dependent Pore Formation and Antimicrobial Activity by Alamethicin and Analogues Duclohier, H. (deepdyve.com)
Transmembrane1
- Alamethicin is a transmembrane ion channel at low concentration, and a lytic agent of cell membrane at high concentration. (rice.edu)
Permeabilization2
- The possibility that taurocholate effects may result from non-specific activation of the overt enzyme was excluded by employing the channel-forming peptide alamethicin to effect permeabilization, and by varying the mode of delivery of diacylglycerol substrate to the microsomal membranes. (biochemj.org)
- Permeabilization using alamethicin gave a slightly higher latent/overt ratio for DGAT. (biochemj.org)
UDPGA1
- 5] . A typical incubation mixture (0.2 mL total volume) contained 50 mM Tris-HCl buffer (pH 7.4), 5 mM MgCl 2 , 5 mM UDPGA, 25 µg/mL alamethicin, 0.25 mg/mL microsomal protein, and 25 - 200 µM morphine. (scirp.org)
Different peptide1
- The IR signature of Alamethicin was determined and different peptide ratios were tested. (uoguelph.ca)
DPhPC1
- The goals of this project were to monitor the incorporation of Alamethicin into 1,2-diphytanoyl-sn-glycero-3-phosphocholine (DPhPC) model biomimetic membranes and characterize the opening and closing of these incorporated Alamethicin channels. (uoguelph.ca)
Orientation2
- A detailed comparison of simulations using different methods to treat long-range electrostatic interactions (a twin range cutoff, Particle Mesh Ewald, and a twin range cutoff combined with a reaction field correction) shows that water orientation inside the alamethicin channels is sensitive to the algorithms used. (ox.ac.uk)
- Polarization Modulation Infrared Reflection Absorption Spectroscopy (PMIRRAS) was used to examine changes in the structure and orientation of Alamethicin as a function of the applied potential. (uoguelph.ca)
Channels5
- It also suggests a possible gating mechanism for alamethicin ion channels. (rice.edu)
- Alamethicin is an antimicrobial peptide that forms stable channels with well-defined conductance levels. (ox.ac.uk)
- Ion channels of N-terminally linked alamethicin dimers: enhancement of cation-selectivity by substitution of Glu for Gln at position 7. (semanticscholar.org)
- article{Okazaki2007IonCO, title={Ion channels of N-terminally linked alamethicin dimers: enhancement of cation-selectivity by substitution of Glu for Gln at position 7. (semanticscholar.org)
- Working with Sol M. Gruner, Keller showed that the conductance state of alamethicin channels changes dramatically when the channels are present in dioleoylphosphatidylethanolamine compared with dioleoylphosphatidylcholine membranes. (asbmb.org)
Interactions2
- I have successfully used alamethicin to study the regulation of the membrane protein callose synthase and its interactions with the fragile cytoskeleton. (avhandlingar.se)
- Continuum solvent model calculations of alamethicin-membrane interactions: thermodynamic aspects. (semanticscholar.org)
Molecular1
- We describe molecular dynamics simulations of the antimicrobial peptide alamethicin in lipid/water and octane/water environments, taking into account an external electric field to mimic the membrane potential. (ox.ac.uk)
Vesicles1
- Attenuated Total Reflection Fourier Transform Infrared spectroscopy (ATR-FTIR) was employed to elucidate the mechanism of Alamethicin incorporation by first spreading vesicles onto the ATR prism. (uoguelph.ca)
Arabidopsis1
- In this thesis alamethicin was used for in situ investigations on Nicotiana tabacum BY-2 and Arabidopsis thaliana cell suspension cells. (avhandlingar.se)
Channel3
- The demonstration of ar- alamethicin [33] was a landmark, from which new struc- tificial membrane excitability developed by planar lipid tural studies sprang also allowing the formulation of hy- bilayers doped with alamethicin [70] indeed struck the potheses on the voltage-dependent membrane insertion minds of many a biophysicist, including electrophysiolo- and the architecture of the oligomeric channel. (deepdyve.com)
- Analysis and evaluation of channel models: simulations of alamethicin. (ox.ac.uk)
- Our findings give new insight into the mechanism of channel formation by alamethicin and the structure and dynamics of membrane-associated helices. (ox.ac.uk)
Helices2
- In a 20-ns simulation of a hexameric alamethicin bundle, the main motions are those of individual helices, not of the bundle as a whole. (ox.ac.uk)
- Polyalanine and alamethicin form stable helices both when inserted in octane and at the water/octane interface, where they partition in the same location. (ox.ac.uk)
Interaction1
- From these two mutually complementary studies we constructed a consistent picture for the interaction between alamethicin and the membrane. (rice.edu)
Resistance2
- The changes may help to explain the cause of alamethicin resistance in cellulase treated cells. (avhandlingar.se)
- With this knowledge the possible advantages for the plant of being able to develop a fungus-induced resistance to a membrane active peptide such as alamethicin could also be clarified. (avhandlingar.se)
Formation1
- When coexisting with T. Viride, plant roots are exposed to alamethicin, which has the ability to elicit formation of volatile compounds involved in pathogen defence. (avhandlingar.se)