AIDS Serodiagnosis
Enzyme-Linked Immunosorbent Assay
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
Sensitivity and Specificity
Antigens, Helminth
Antigens, Protozoan
Immunoglobulin M
Immunoglobulin G
Lyme Disease
An infectious disease caused by a spirochete, BORRELIA BURGDORFERI, which is transmitted chiefly by Ixodes dammini (see IXODES) and pacificus ticks in the United States and Ixodes ricinis (see IXODES) in Europe. It is a disease with early and late cutaneous manifestations plus involvement of the nervous system, heart, eye, and joints in variable combinations. The disease was formerly known as Lyme arthritis and first discovered at Old Lyme, Connecticut.
Agglutination Tests
Borrelia burgdorferi Group
Gram-negative helical bacteria, in the genus BORRELIA, that are the etiologic agents of LYME DISEASE. The group comprises many specific species including Borrelia afzelii, Borellia garinii, and BORRELIA BURGDORFERI proper. These spirochetes are generally transmitted by several species of ixodid ticks.
Evaluation Studies as Topic
Complement Fixation Tests
Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.
Hemagglutination Tests
Dourine
Leishmaniasis, Visceral
A chronic disease caused by LEISHMANIA DONOVANI and transmitted by the bite of several sandflies of the genera Phlebotomus and Lutzomyia. It is commonly characterized by fever, chills, vomiting, anemia, hepatosplenomegaly, leukopenia, hypergammaglobulinemia, emaciation, and an earth-gray color of the skin. The disease is classified into three main types according to geographic distribution: Indian, Mediterranean (or infantile), and African.
Leishmania infantum
A parasitic hemoflagellate of the subgenus Leishmania leishmania that infects man and animals and causes visceral leishmaniasis (LEISHMANIASIS, VISCERAL). Human infections are confined almost entirely to children. This parasite is commonly seen in dogs, other Canidae, and porcupines with humans considered only an accidental host. Transmission is by Phlebotomus sandflies.
Cross Reactions
Glanders
Immunoblotting
Gnathostomiasis
Dog Diseases
Fascioliasis
Clonorchis sinensis
Echinococcosis, Pulmonary
Clonorchiasis
Infection of the biliary passages with CLONORCHIS SINENSIS, also called Opisthorchis sinensis. It may lead to inflammation of the biliary tract, proliferation of biliary epithelium, progressive portal fibrosis, and sometimes bile duct carcinoma. Extension to the liver may lead to fatty changes and cirrhosis. (From Dorland, 27th ed)
Reagent Kits, Diagnostic
Fasciola
Reagins
Central Nervous System Parasitic Infections
Taenia solium
Liver Abscess, Amebic
Babesia
Latex Fixation Tests
Acquired Immunodeficiency Syndrome
An acquired defect of cellular immunity associated with infection by the human immunodeficiency virus (HIV), a CD4-positive T-lymphocyte count under 200 cells/microliter or less than 14% of total lymphocytes, and increased susceptibility to opportunistic infections and malignant neoplasms. Clinical manifestations also include emaciation (wasting) and dementia. These elements reflect criteria for AIDS as defined by the CDC in 1993.
Veterinary Medicine
Burkholderia mallei
Borrelia
Neospora
A genus of protozoan parasites of the subclass COCCIDIA. Its species are parasitic in dogs, cattle, goats, and sheep, among others. N. caninum, a species that mainly infects dogs, is intracellular in neural and other cells of the body, multiplies by endodyogeny, has no parasitophorous vacuole, and has numerous rhoptries. It is known to cause lesions in many tissues, especially the brain and spinal cord as well as abortion in the expectant mother.
Ehrlichiosis
A tick-borne disease characterized by FEVER; HEADACHE; myalgias; ANOREXIA; and occasionally RASH. It is caused by several bacterial species and can produce disease in DOGS; CATTLE; SHEEP; GOATS; HORSES; and humans. The primary species causing human disease are EHRLICHIA CHAFFEENSIS; ANAPLASMA PHAGOCYTOPHILUM; and Ehrlichia ewingii.
Chagas Disease
Infection with the protozoan parasite TRYPANOSOMA CRUZI, a form of TRYPANOSOMIASIS endemic in Central and South America. It is named after the Brazilian physician Carlos Chagas, who discovered the parasite. Infection by the parasite (positive serologic result only) is distinguished from the clinical manifestations that develop years later, such as destruction of PARASYMPATHETIC GANGLIA; CHAGAS CARDIOMYOPATHY; and dysfunction of the ESOPHAGUS or COLON.
Toxocariasis
Reagent Strips
Fluorescent Antibody Technique
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Melioidosis
Fasciola hepatica
Yaws
Leptospira
Babesiosis
Ehrlichia chaffeensis
Gnathostoma
Sparganosis
Infection of animals, including fish and man, with a developmental stage of Diphyllobothrium. This stage has recently been referred to as a plerocercoid but the name sparganum has persisted. Therefore, infection of fish or other animals with the plerocercoid larvae is sparganosis. Fish-eating mammals, including man, are the final hosts.
Borrelia burgdorferi
Pythium
Counterimmunoelectrophoresis
Neurocysticercosis
Infection of the brain, spinal cord, or perimeningeal structures with the larval forms of the genus TAENIA (primarily T. solium in humans). Lesions formed by the organism are referred to as cysticerci. The infection may be subacute or chronic, and the severity of symptoms depends on the severity of the host immune response and the location and number of lesions. SEIZURES represent the most common clinical manifestation although focal neurologic deficits may occur. (From Joynt, Clinical Neurology, 1998, Ch27, pp46-50)
Salmonella paratyphi C
Echinococcosis
Immunoenzyme Techniques
Entamoeba histolytica
Paragonimus
Paracoccidioides
Brucella
Fluorescent Antibody Technique, Indirect
A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. The technique involves formation of an antigen-antibody complex which is labeled with fluorescein-conjugated anti-immunoglobulin antibody. (From Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)
Paracoccidioidomycosis
Toxoplasma
Cysticercosis
Trypanosoma cruzi
Entamoebiasis
Trichinellosis
An infection with TRICHINELLA. It is caused by eating raw or undercooked meat that is infected with larvae of nematode worms TRICHINELLA genus. All members of the TRICHINELLA genus can infect human in addition to TRICHINELLA SPIRALIS, the traditional etiological agent. It is distributed throughout much of the world and is re-emerging in some parts as a public health hazard and a food safety problem.
Immunoassay
False Positive Reactions
Echinococcus
Typhoid Fever
Treponema Immobilization Test
Coccidiosis
Antibody Specificity
Ehrlichia
Immunoconglutinins
Brucellosis
Burkholderia pseudomallei
Immunoglobulin A
Erythema Chronicum Migrans
A deep type of gyrate erythema that follows a bite by an ixodid tick; it is a stage-1 manifestation of LYME DISEASE. The site of the bite is characterized by a red papule that expands peripherally as a nonscaling, palpable band that clears centrally. This condition is often associated with systemic symptoms such as chills, fever, headache, malaise, nausea, vomiting, fatigue, backache, and stiff neck.
Bacterial Outer Membrane Proteins
Mycobacterium tuberculosis
Leishmania donovani
Clinical Laboratory Techniques
Blotting, Western
Plague
Immunodiffusion
Echinococcus granulosus
Toxocara canis
Cattle Diseases
Dogs
Pleuropneumonia
Hemagglutination Inhibition Tests
Glycolipids
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Leishmania
A genus of flagellate protozoa comprising several species that are pathogenic for humans. Organisms of this genus have an amastigote and a promastigote stage in their life cycles. As a result of enzymatic studies this single genus has been divided into two subgenera: Leishmania leishmania and Leishmania viannia. Species within the Leishmania leishmania subgenus include: L. aethiopica, L. arabica, L. donovani, L. enrietti, L. gerbilli, L. hertigi, L. infantum, L. major, L. mexicana, and L. tropica. The following species are those that compose the Leishmania viannia subgenus: L. braziliensis, L. guyanensis, L. lainsoni, L. naiffi, and L. shawi.
Trichinella spiralis
First Aid
Immunodominant Epitopes
Fluorescent Treponemal Antibody-Absorption Test
Serologic assay that detects antibodies to Treponema pallidum, the etiologic agent of syphilis. After diluting the patient's serum to remove non-specific antibodies, the serum is mixed on a glass slide with Nichol's strain of Treponema pallidum. An antigen-antibody reaction occurs if the test is positive and the bound antibodies are detected with fluoresceinated antihuman gamma-globulin antibody.
Scrub Typhus
An acute infectious disease caused by ORIENTIA TSUTSUGAMUSHI. It is limited to eastern and southeastern Asia, India, northern Australia, and the adjacent islands. Characteristics include the formation of a primary cutaneous lesion at the site of the bite of an infected mite, fever lasting about two weeks, and a maculopapular rash.
Cloning, Molecular
Horses
Heartwater Disease
Tuberculosis
Predictive Value of Tests
In screening and diagnostic tests, the probability that a person with a positive test is a true positive (i.e., has the disease), is referred to as the predictive value of a positive test; whereas, the predictive value of a negative test is the probability that the person with a negative test does not have the disease. Predictive value is related to the sensitivity and specificity of the test.
Ehrlichia ruminantium
Weil Disease
Leishmaniasis
A disease caused by any of a number of species of protozoa in the genus LEISHMANIA. There are four major clinical types of this infection: cutaneous (Old and New World) (LEISHMANIASIS, CUTANEOUS), diffuse cutaneous (LEISHMANIASIS, DIFFUSE CUTANEOUS), mucocutaneous (LEISHMANIASIS, MUCOCUTANEOUS), and visceral (LEISHMANIASIS, VISCERAL).
Reproducibility of Results
The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.
Nucleocapsid Proteins
Amino Acid Sequence
Leprosy
Flagellin
Recombinant Fusion Proteins
Antigens, Surface
Mycobacterium leprae
Immune Adherence Reaction
A method for the detection of very small quantities of antibody in which the antigen-antibody-complement complex adheres to indicator cells, usually primate erythrocytes or nonprimate blood platelets. The reaction is dependent on the number of bound C3 molecules on the C3b receptor sites of the indicator cell.
Neutralization Tests
The measurement of infection-blocking titer of ANTISERA by testing a series of dilutions for a given virus-antiserum interaction end-point, which is generally the dilution at which tissue cultures inoculated with the serum-virus mixtures demonstrate cytopathology (CPE) or the dilution at which 50% of test animals injected with serum-virus mixtures show infectivity (ID50) or die (LD50).
Swine
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
Antibodies
Leptospira interrogans
Coccidioidomycosis
Infection with a fungus of the genus COCCIDIOIDES, endemic to the SOUTHWESTERN UNITED STATES. It is sometimes called valley fever but should not be confused with RIFT VALLEY FEVER. Infection is caused by inhalation of airborne, fungal particles known as arthroconidia, a form of FUNGAL SPORES. A primary form is an acute, benign, self-limited respiratory infection. A secondary form is a virulent, severe, chronic, progressive granulomatous disease with systemic involvement. It can be detected by use of COCCIDIOIDIN.
Flagella
A whiplike motility appendage present on the surface cells. Prokaryote flagella are composed of a protein called FLAGELLIN. Bacteria can have a single flagellum, a tuft at one pole, or multiple flagella covering the entire surface. In eukaryotes, flagella are threadlike protoplasmic extensions used to propel flagellates and sperm. Flagella have the same basic structure as CILIA but are longer in proportion to the cell bearing them and present in much smaller numbers. (From King & Stansfield, A Dictionary of Genetics, 4th ed)
HIV Infections
Whooping Cough
Aspergillus fumigatus
Endemic Diseases
The constant presence of diseases or infectious agents within a given geographic area or population group. It may also refer to the usual prevalence of a given disease with such area or group. It includes holoendemic and hyperendemic diseases. A holoendemic disease is one for which a high prevalent level of infection begins early in life and affects most of the child population, leading to a state of equilibrium such that the adult population shows evidence of the disease much less commonly than do children (malaria in many communities is a holoendemic disease). A hyperendemic disease is one that is constantly present at a high incidence and/or prevalence rate and affects all groups equally. (Last, A Dictionary of Epidemiology, 3d ed, p53, 78, 80)
Electrophoresis, Polyacrylamide Gel
Sheep
Protein Array Analysis
Chromatography
Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. Usage is both analytical for small amounts and preparative for bulk amounts.
Rabbits
Epitope Mapping
Goats
Cattle
Base Sequence
Sequence Analysis, DNA
Seroepidemiologic Studies
Polymerase Chain Reaction
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Escherichia coli
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Species Specificity
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Lipoproteins
Lipid-protein complexes involved in the transportation and metabolism of lipids in the body. They are spherical particles consisting of a hydrophobic core of TRIGLYCERIDES and CHOLESTEROL ESTERS surrounded by a layer of hydrophilic free CHOLESTEROL; PHOSPHOLIPIDS; and APOLIPOPROTEINS. Lipoproteins are classified by their varying buoyant density and sizes.
Helicobacter Infections
Infections with organisms of the genus HELICOBACTER, particularly, in humans, HELICOBACTER PYLORI. The clinical manifestations are focused in the stomach, usually the gastric mucosa and antrum, and the upper duodenum. This infection plays a major role in the pathogenesis of type B gastritis and peptic ulcer disease.
Antigen-Antibody Reactions
Peptides
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
Mycobacterium bovis
Baculoviridae
Family of INSECT VIRUSES containing two subfamilies: Eubaculovirinae (occluded baculoviruses) and Nudibaculovirinae (nonoccluded baculoviruses). The Eubaculovirinae, which contain polyhedron-shaped inclusion bodies, have two genera: NUCLEOPOLYHEDROVIRUS and GRANULOVIRUS. Baculovirus vectors are used for expression of foreign genes in insects.
Immune Sera
Human immunodeficiency virus antibody testing by enzyme-linked fluorescent and western blot assays using serum, gingival-crevicular transudate, and urine samples. (1/692)
The aim of the present study was to evaluate the possible utilization of saliva and urine as alternative samples to serum for the diagnosis of human immunodeficiency virus (HIV) infection. A total of 302 individuals participated in the study: 187 HIV-infected individuals (106 had Centers for Disease Control and Prevention [CDC] stage II infection, 19 had CDC stage III infection, and 62 had CDC stage IV infection) and 115 noninfected persons (46 of the noninfected persons were blood donors and 69 belonged to a group at high risk of HIV infection). Paired saliva and urine samples were taken from each of the participants in the study. The presence of HIV-specific antibodies was detected by an enzyme-linked fluorescent assay (ELFA), and the result was confirmed by Western blot analysis (WB). The ELFA with saliva gave maximum sensitivity and specificity values, while ELFA had lower sensitivity (95.2%) and specificity (97. 4%) values for detection of HIV antibody in urine samples. WB with all saliva samples fulfilled the World Health Organization criterion for positivity, while only 96.8% of the urine samples were confirmed to be positive by WB. Among the four reactivity patterns found by WB of these alternative samples, the most frequent included bands against three groups of HIV structural proteins (was ENV, POL, and GAG). The reactivity bands most frequently observed were those for the proteins gp160 and gp120. The least common reactivity band was the band for protein p17. The detection of HIV antibodies in saliva samples by means of ELFA with the possibility of later confirmation by WB makes saliva an alternative to serum for possible use in the diagnosis of infection. In contrast, HIV antibody detection in urine samples by the same methodology (ELFA) could be taken into consideration for use in epidemiological studies. (+info)Prenatal discussion of HIV testing and maternal HIV testing--14 states, 1996-1997. (2/692)
In July 1995, the Public Health Service recommended that health-care providers counsel all pregnant women about human immunodeficiency virus (HIV) prevention and encourage testing for HIV infection and, if indicated, initiate zidovudine therapy. To evaluate compliance with these recommendations, CDC analyzed population-based data on HIV counseling and testing during 1996-1997 from 14 states participating in the Pregnancy Risk Assessment Monitoring System (PRAMS). This report presents an analysis of survey data collected from 1996 through 1997; results indicate that HIV counseling and testing of pregnant women were common but varied by state, type of prenatal health-care provider, Medicaid status, and maternal demographic characteristics. (+info)Universal HIV screening of pregnant women in England: cost effectiveness analysis. (3/692)
OBJECTIVE: To estimate the cost effectiveness of universal, voluntary HIV screening of pregnant women in England. DESIGN: Cost effectiveness analysis. Cost estimates of caring for HIV positive children were based on the stage of HIV infection and calculated using data obtained from a London hospital between 1986 and 1996. These were combined with estimates of the health benefits and costs of antenatal screening so that the cost effectiveness of universal, voluntary antenatal screening for HIV infection in England could be estimated. MAIN OUTCOME MEASURES: Lifetime, direct costs of medical care of childhood HIV infection; life years gained as a result of the screening programme; net cost per life year gained for different pretest counselling costs; and different prevalence rates of pregnant women who were unaware that they were HIV positive. RESULTS: Estimated direct lifetime medical and social care costs of childhood HIV infection were pound178 300 using a 5% discount rate for time preference (1995-6 prices). In high prevalence areas screening pregnant women for HIV is estimated to be a cost effective intervention with a net cost of less than pound4000 for each life year gained. For areas with comparatively low prevalence rates, cost effectiveness could be less than pound20 000 per life year gained, depending on the number of pregnant women who are unaware that they are infected and local screening costs. CONCLUSIONS: Our results confirm recent recommendations that universal, voluntary antenatal HIV screening should be implemented in the London area. Serious consideration of the policy should be given for other areas in England depending on local prevalence and screening costs. (+info)Name-based reporting of HIV-positive test results as a deterrent to testing. (4/692)
OBJECTIVES: This study evaluated attitudes toward name-based reporting of HIV. METHODS: One hundred thirty high-risk, male repeat testers received information on the public health benefits of name-based reporting and reported their intentions to test. RESULTS: Of the 67 men who were randomly selected and asked their intentions before hearing the benefits, 63% said they would not test if reporting were required. After hearing the benefits, 19% changed their minds (P < .014). Of the 63 men who were asked only after hearing the benefits, 44% would not test. CONCLUSIONS: Implementing name-based reporting without working before-hand to change attitudes could undermine the benefits of both testing and HIV surveillance. (+info)Anonymous or confidential HIV counseling and voluntary testing in federally funded testing sites--United States, 1995-1997. (5/692)
Human immunodeficiency virus (HIV) counseling and voluntary testing (CT) programs have been an important part of national HIV prevention efforts since the first HIV antibody tests became available in 1985. In 1995, these programs accounted for approximately 15% of annual HIV antibody testing in the United States, excluding testing for blood donation. CT opportunities are offered to persons at risk for HIV infection at approximately 11,000 sites, including dedicated HIV CT sites, sexually transmitted disease (STD) clinics, drug-treatment centers, hospitals, and prisons. In 39 states, testing can be obtained anonymously, where persons do not have to give their name to get tested. All states provide confidential testing (by name) and have confidentiality laws and regulations to protect this information. This report compares patterns of anonymous and confidential testing in all federally funded CT programs from 1995 through 1997 and documents the importance of both types of testing opportunities. (+info)Effects of HIV counseling and testing on sexual risk behavior: a meta-analytic review of published research, 1985-1997. (6/692)
OBJECTIVES: This study examined whether HIV counseling and testing leads to reductions in sexual risk behavior. METHODS: The meta-analysis included 27 published studies that provided sexual behavior outcome data, assessed behavior before and after counseling and testing, and provided details sufficient for the calculation of effect sizes. The studies involved 19,597 participants. RESULTS: After counseling and testing, HIV-positive participants and HIV-serodiscordant couples reduced unprotected intercourse and increased condom use more than HIV-negative and untested participants. HIV-negative participants did not modify their behavior more than untested participants. Participants' age, volition for testing, and injection drug use treatment status, as well as the sample seroprevalence and length of the follow-up, explained the variance in results. CONCLUSIONS: HIV counseling and testing appears to provide an effective means of secondary prevention for HIV-positive individuals but, as conducted in the reviewed studies, is not an effective primary prevention strategy for uninfected participants. Theory-driven research with attention given to the context of testing is needed to further explicate the determinants of behavior change resulting from HIV counseling and testing, and the effectiveness of specific counseling approaches. (+info)Field evaluation of the Determine rapid human immunodeficiency virus diagnostic test in Honduras and the Dominican Republic. (7/692)
Rapid detection of human immunodeficiency virus (HIV) infection can result in improved patient care and/or faster implementation of public health preventive measures. A new rapid test, Determine (Abbott, Abbott Park, Ill.), detects HIV type 1 (HIV-1) and HIV-2 antibodies within 15 min by using 50 microl of serum or plasma. No specialized equipment or ancillary supplies are required, and results are read visually. A positive result is noted by the appearance of a red line. An operational control (red line) indicates proper test performance. We evaluated the Determine rapid HIV detection test with a group of well-characterized serum samples (CD4 counts and viral loads were known) and serum samples from HIV-positive individuals at field sites in Honduras and the Dominican Republic. In the field evaluations, the results obtained by the Determine assay were compared to those obtained by local in-country HIV screening procedures. We evaluated serum from 100 HIV-positive patients and 66 HIV-negative patients. All samples gave the expected results. In a companion study, 42 HIV-positive samples from a Miami, Fla., serum bank were tested by the Determine assay. The samples had been characterized in terms of CD4 counts and viral loads. Fifteen patients had CD4 counts <200 cells/mm(3), while 27 patients had CD4 counts >200 cells/mm(3). Viral loads ranged from 630 to 873,746 log(10) copies/ml. All samples from the Miami serum bank were positive by the Determine test. Combined results from the multicenter studies indicated that the correct results were obtained by the Determine assay for 100% (142 of 142) of the HIV-positive serum samples and 100% (66 of 66) of the HIV-negative serum samples. The Determine test was simple to perform and the results were easy to interpret. The Determine test provides a valuable new method for the rapid identification of HIV-positive individuals, especially in developing countries with limited laboratory infrastructures. (+info)Serological diagnosis of human immuno-deficiency virus in Burkina Faso: reliable, practical strategies using less expensive commercial test kits. (8/692)
Reported are the results of a cross-sectional survey in Burkina Faso to identify reliable, practical strategies for the serological diagnosis of HIV-1 and/or HIV-2 infections, using less-expensive commercial test kits in various combinations, as an alternative to the conventional Western blot (WB) test, which costs US$ 60. Serum samples, collected from blood donors, patients with acquired immunodeficiency syndrome (AIDS) and pregnant women, were tested between December 1995 and January 1997. Twelve commercial test kits were available: five Mixt enzyme-linked immunosorbent assays (ELISA), three Mixt rapid tests, and four additional tests including monospecific HIV-1 and HIV-2 ELISA. The reference strategy utilized a combination of one ELISA or one rapid test with WB, and was conducted following WHO criteria. A total of 768 serum samples were tested; 35 were indeterminate and excluded from the analysis. Seroprevalence of HIV in the remaining 733 sera was found to be 37.5% (95% confidence interval: 34.0-41.1). All the ELISA tests showed 100% sensitivity, but their specificities ranged from 81.4% to 100%. GLA (Genelavia Mixt) had the highest positive delta value, while ICE HIV-1.0.2 (ICE) produced the most distinct negative results. Among the rapid tests, COM (CombAIDS-RS) achieved 100% sensitivity and SPO (HIV Spot) 100% specificity. Various combinations of commercial tests, according to recommended WHO strategies I, II, III, gave excellent results when ICE was included in the sequence. The best combination of tests for strategy II, which achieved 100% sensitivity and specificity, was to use ICE and COM, the cost of which was US$ 2.10, compared with US$ 55.60 for the corresponding conventional strategy. For strategy III, the best combination, which achieved 100% sensitivity and specificity, was to use ICE, ZYG (Enzygnost Anti HIV-1/HIV-2 Plus) and COM, the cost of which was US$ 2.90 (19.2 times lower than the corresponding strategy requiring WB). No rapid test combination showed 100% sensitivity and specificity. Our results indicate that the serodiagnosis of HIV in Burkina Faso is possible by using reliable, less-expensive strategies which do not require Western blot testing. Moreover, there is a choice of strategies for laboratories working with or without an ELISA chain. (+info)
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List of MeSH codes (E01)
... aids serodiagnosis MeSH E01.450.495.735.150 - complement fixation tests MeSH E01.450.495.735.155 - complement hemolytic ... syphilis serodiagnosis MeSH E01.450.495.735.850.200 - fluorescent treponemal antibody absorption test MeSH E01.450.495.735. ...
Strongyloidiasis
Kramer MR, Gregg PA, Goldstein M, Llamas R, Krieger BP (October 1990). "Disseminated strongyloidiasis in AIDS and non-AIDS ... Carroll SM, Karthigasu KT, Grove DI (1981). "Serodiagnosis of human strongyloidiasis by an enzyme-linked immunosorbent assay". ... Gompels MM, Todd J, Peters BS, Main J, Pinching AJ (March 1991). "Disseminated strongyloidiasis in AIDS: uncommon but important ... ". AIDS. 5 (3): 329-32. doi:10.1097/00002030-199103000-00015. PMID 2059374. Purtilo DT, Meyers WM, Connor DH (April 1974). " ...
Serology
eds.). Principles of Diagnosis: Serodiagnosis. in: Baron's Medical Microbiology (4th ed.). Univ of Texas Medical Branch. ISBN ... aid agencies, and policy writers to direct public health resources to where they are needed most. A better understanding of ...
Virology
May 1983). "Isolation of a T-lymphotropic retrovirus from a patient at risk for acquired immune deficiency syndrome (AIDS)". ... Swack NS, Gahan TF, Hausler WJ (August 1992). "The present status of the complement fixation test in viral serodiagnosis". ... Journal of the International AIDS Society. 22 (9): e25337. doi:10.1002/jia2.25337. PMC 6742838. PMID 31515967. Shirima RR, ...
Temple University School of Medicine
... serodiagnosis and chemotherapy. Frank H. Krusen, originator of the field of physical medicine, establishing the first such ... and utilizes the William Maul Measey Institute for Clinical Simulation and Patient Safety to aid learning through interactive ...
Chronic pulmonary aspergillosis
Coleman RM, Kaufman L (February 1972). "Use of the immunodiffusion test in the serodiagnosis of aspergillosis". Applied ... chronic obstructive pulmonary disease or HIV/AIDS. In contrast to chronic cavitary pulmonary aspergillosis, for example, IgG ...
Browsing Information products by Subject "AIDS Serodiagnosis"
Browsing Publications by Subject "AIDS Serodiagnosis"
Results of search for 'su:{AIDS Serodiagnosis}' › WHO HQ Library catalog
of HIV/AIDS , International HIV/AIDS Alliance.. Material type: Text; Format: print ; Literary form: Not fiction Publication ... by UNAIDS/WHO Working Group on Global HIV/AIDS/STI Surveillance , World Health Organization. Dept. of HIV/AIDS , Centers for ... UNAIDS/WHO Working Group on Global HIV/AIDS and STI Surveillance. by UNAIDS/WHO Working Group on Global HIV/AIDS/STI ... of HIV/AIDS , Centers for Disease Control (U.S.).. Material type: Text; Format: print Publication details: Geneva : World ...
Browsing Meeting reports by Subject
Browsing Information products by Subject
MESH TREE NUMBER CHANGES - 2008 MeSH
Use of Urea Wash ELISA to Distinguish Zika and Dengue Virus Infections - Volume 24, Number 7-July 2018 - Emerging Infectious...
Recommendations for HIV Screening of Gay, Bisexual, and Other Men Who Have Sex with Men - United States, 2017 | MMWR
AIDS serodiagnosis, sexually transmitted diseases/infections, men who have sex with men (MSM), high risk, test, screen. ... 1Division of HIV/AIDS Prevention, National Center for HIV/AIDS, Viral Hepatitis, STD, and TB Prevention, CDC. ... 2. Atlanta, GA: US Department of Health and Human Services, CDC, National Center for HIV/AIDS, Viral Hepatitis, STD, and TB, ... Division of HIV/AIDS Prevention; 2017. https://www.cdc.gov/hiv/pdf/library/reports/surveillance/cdc-hiv-surveillance- ...
Portal Regional da BVS
Interpretation and Use of the Western Blot Assay for Serodiagnosis o
... f Human Immunodeficiency Virus Type 1 Infections ... Antigenemia and antibody titers to core and envelope antigens in AIDS, AIDS-related complex, and subclinical human ... As the HIV/AIDS epidemic continues, additional tests of higher specificity will be needed to decrease the number of false- ... AIDS: Etiology, Treatment, and Prevention, Second Edition. Philadelphia: J.B. Lippincott, 1988:121-6. *Fang C, Le P, Mallory D ...
DeCS
AIDS Dementia Complex MeSH AIDS Serodiagnosis MeSH HIV Seropositivity MeSH HIV Seroprevalence MeSH Lymphoma, AIDS-Related MeSH ... for lymphoma with AIDS, use LYMPHOMA, AIDS-RELATED. ... AIDS Related Complex [C01.221.250.875.080] AIDS Related Complex ... AIDS Acquired Immune Deficiency Syndrome Acquired Immuno Deficiency Syndrome Acquired Immuno-Deficiency Syndrome Acquired ... caused by HIV; coord IM with HIV-1 or HIV-2 (IM) if pertinent; /epidemiol: consider also HIV SEROPREVALENCE; AIDS-RELATED ...
ns
IMSEAR at SEARO: Human immunodeficiency virus-1 infection in spouses of seropositive individuals.
DeCS
AIDS Serodiagnosis Entry term(s). AIDS Serodiagnoses AIDS Serology HIV Serodiagnoses HIV Serodiagnosis HTLV III Serodiagnosis ... Serodiagnosis, AIDS Serodiagnosis, HIV Serodiagnosis, HTLV III Serodiagnosis, HTLV-III Serology, AIDS Serology, HTLV III ... Serodiagnosis, AIDS. Serodiagnosis, HIV. Serodiagnosis, HTLV III. Serodiagnosis, HTLV-III. Serology, AIDS. Serology, HTLV III. ... AIDS Serodiagnoses. AIDS Serology. HIV Serodiagnoses. HIV Serodiagnosis. HTLV III Serodiagnosis. HTLV III Serology. HTLV-III ...
BIGG
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MESH TREE NUMBER CHANGES - 2008 MeSH
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Acquired immunodeSeropositivitySyphilisAssayInfectionELISALymeVisceralAntibodiesDiagnosisCentersPatientsTransmissionRecipientsDiseaseTestMethodsInvolvementCasesIndividualsVirusNewsMaleInfectionsAntigensAsymptomatic1991SyphilisAcquired ImmunodePreventionDengueSensitivityDischargeCOMPLEXProgramVarietyResearchPeopleLife
Acquired immunode3
- The development of sensitive and specific tests for antibody to human immunodeficiency virus type 1 (HIV-1) progressed rapidly after this retrovirus was identified as the cause of acquired immunodeficiency syndrome (AIDS). (cdc.gov)
- Methods: Newly diagnosed 78 patients with HIV/AIDS (Acquired Immunodeficiency Syndrome) in our outpatient department between April 2009 and September 2012 were included in the study as a retrospective cohort. (jsurgmed.com)
- Other patients, often older persons or those with immune compromise states (such as patients with human immunodeficiency virus infection / acquired immunodeficiency syndrome [HIV/AIDS] or solid organ transplant recipients) may present with an acute, rapidly progressive, severe disease. (medscape.com)
Seropositivity1
- They include assays for HIV SEROPOSITIVITY and HIV SERONEGATIVITY that have been developed for screening persons carrying the viral antibody from patients with overt symptoms of AIDS or AIDS-RELATED COMPLEX. (bvsalud.org)
Syphilis1
- Then, 29 of these patients diagnosed with syphilis, by combining the serodiagnosis and disease history (including clinical characteristics and/or the patient's sexual history) according to European CDCs guidelines [ 8 ], were assigned to the syphilis-positive schizophrenia (SCZ-S) group. (biomedcentral.com)
Assay1
- To identify PDCoV, we developed a nucleic acid detection assay combining reverse transcription recombinase-aided amplification (RT-RAA) with a lateral flow dipstick (LFD) targeting the highly conserved genomic region in the ORF1b gene. (bvsalud.org)
Infection4
- RÉSUMÉ Dans cette étude en République islamique d'Iran, 365 cas de rougeole ont été évalués pour distinguer une primo-infection rougeoleuse de la réinfection due à l'échec de la vaccination secondaire. (who.int)
- [ 26 ] Sporotrichosis in persons with AIDS can also manifest as multifocal tenosynovitis or arthritis resembling disseminated gonococcal infection or a seronegative spondyloarthropathy. (medscape.com)
- https://clinicalinfo.hiv.gov/en/guidelines/adult-and-adolescent-opportunistic-infection/whats-new-guidelines (Access Date: April 26, 2020). (jsurgmed.com)
- 3 Pandemic of AIDS has virtually acted as trigger to rapid spread of penicilliosis marneffei as opportunistic infection or secondary infection. (medcraveonline.com)
ELISA1
- Prosolit, one of the leading companies that are involved in the provision of diagnostic products and technologies for veterinary infectious disease testing, offers a technology called the "ELISA kit for serodiagnosis of Bovine Adenovirus3 ", which is a test designed for the diagnosis of this virus in the cattle. (knowledge-sourcing.com)
Lyme2
- Did you know this figure is six times higher than HIV/AIDS, yet only about 10% of the Lyme cases are allowed diagnosis and treatment? (badlymeattitude.com)
- Standard 2-tier and modified 2-tier algorithms for serodiagnosis of Lyme disease. (cdc.gov)
Visceral1
- Patients with AIDS who develop disseminated sporotrichosis may present with cutaneous dissemination, which manifests as nodules, ulcers, or both, with or without evidence of visceral involvement and meningitis. (medscape.com)
Antibodies1
- Antibodies produced through B and T cell interactions either within or outside germinal centers are termed T cell-dependent (TD) whereas those produced without the aid of T cells are T cell-independent (TI). (madisonarealymesupportgroup.com)
Diagnosis2
- HIV/AIDS Diagnosis Treatment Guide - 2019. (jsurgmed.com)
- The better, advanced, and diverse varieties of veterinary infectious disease diagnostics with enhanced features can effectively aid in the proper and efficient diagnosis and detection of diseases. (knowledge-sourcing.com)
Centers2
- Reported by: Association of State and Territorial Public Health Laboratory Directors and AIDS Program, Center for Infectious Diseases, Public Health Practice Program Office, Centers for Disease Control* The Association of State and Territorial Public Health Laboratory Directors (ASTPHLD) and CDC have collaborated in preparing this report. (cdc.gov)
- Estos elementos reflejan los criterios de SIDA definidos por los CDC (Centers for Disease Control and Prevention) en 1993. (bvsalud.org)
Patients5
- Pulmonary toxoplasmosis (pneumonitis) due to toxoplasmosis is increasingly recognized in patients with AIDS who are not receiving appropriate anti-HIV drugs or primary prophylaxis for toxoplasmosis. (medscape.com)
- Pulmonary toxoplasmosis occurs mainly in patients with advanced AIDS (mean CD4 + count of 40 cells/µL ±75 standard deviation) and primarily manifests as a prolonged febrile illness with cough and dyspnea. (medscape.com)
- AIDS patients, in the beginning, had the luxury of death. (badlymeattitude.com)
- Summary Sera from patients with likely and possible Pneumocystis carinii pneumonia (PCP) on the basis of clinical information and laboratory investigations were tested by immunoblotting to assess the usefulness of trophozoites in the serodiagnosis of PCP. (lookformedical.com)
- It is also known as AIDS defining illness among patients who have either lived or visited endemic areas regardless of time period since exposure. (medcraveonline.com)
Transmission1
- The story focuses on situations relating to AIDS, including facts about the AIDS virus, symptoms and transmission of AIDS, use of condoms, and AIDS screening. (nih.gov)
Recipients1
- This increase is directly related to increasing patient populations at risk for the development of infections, which include the elderly, premature babies, solid-organ and bone marrow transplant recipients, individuals with AIDS, neoplastic disease and those receiving immunosuppressive therapies or cytotoxic drugs. (exeter.ac.uk)
Disease2
- The truth is that most Lymies are walking around with an AIDS-like disease, pretending with all their heart to be functional, while fully knowing that on the inside, everything is disintegrating into a puddle of useless sludge. (badlymeattitude.com)
- They are barely hanging on, working to support a family, pay the mortgage, cover medical bills that are denied by insurance, put on a show of everything's-rainbows-and-unicorns, all while harboring this AIDS-like disease that is killing them from the top down, from the inside out. (badlymeattitude.com)
Test1
- This test will aid serodiagnosis, serosurveillance, and monitoring of Zika complications in dengue-endemic regions. (cdc.gov)
Methods1
- Methods We analysed data from adolescents aged 15-18 years, who participated in Demographic and Health Surveys or AIDS Indicator Surveys between 2011 and 2016, in 15 sub-Saharan African countries. (bvsalud.org)
Involvement2
- Brain involvement (ie, toxoplasmic encephalitis), with or without focal CNS lesions, is the most common manifestation of toxoplasmosis in individuals with AIDS. (medscape.com)
- Central nervous system and meningeal involvement are more common in the AIDS era, but it remains rare. (medscape.com)
Cases1
- Results: Of the 78 HIV/AIDS cases, 68 (87.2%) were male. (jsurgmed.com)
Individuals1
- [ 3 ] C jejuni infections also may produce serious bacteremic conditions in individuals with AIDS . (medscape.com)
Virus1
- Can gene therapy be harnessed to fight the AIDS virus? (cdc.gov)
News1
- This image represents the cover of the comic book 'AIDS news,' an AIDS education tool written in English. (nih.gov)
Male1
- Since 2007 the World Health Organization (WHO) and the Joint United Nations Programme on HIV/AIDS (UNAIDS) have recommended voluntary medical male circumcision (VMMC) as an important strategy for the prevention of heterosexually acquired HIV in men in settings where the prevalence of heterosexually trans. (bvsalud.org)
Infections1
- This increase is directly related to increasing patient populations at risk for the development of infections, which include the elderly, premature babies, solid-organ and bone marrow transplant recipients, individuals with AIDS, neoplastic disease and those receiving immunosuppressive therapies or cytotoxic drugs. (exeter.ac.uk)
Antigens3
- Since a single antigen is not effective for all endemic areas, newer antigens have been screened and evaluated for the serodiagnosis of VL. (biomedcentral.com)
- In order to aid in the development of simple diagnostic approaches, identification and characterization of potential new antigens for immunodiagnostic purposes is desired. (edu.pe)
- Many mycobacterial antigens have been evaluated for the serodiagnosis of TB and have been extensively examined elsewhere, although data in pediatric populations are very limited [21C24]. (chiflatironsofficial.com)
Asymptomatic1
- The acute phase is typically followed by an asymptomatic carrier, which progresses to clinical AIDS in about 50% of infected individuals within 10 years after seroconversion. (rapidtest.com)
19911
- http://worldcatlibraries.org/registry/gateway?version=1.0&url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&atitle=The+Knowledge,+Attitudes+and+Concerns+of+Hospital+Staff+About+aids&title=Canadian+Journal+of+Public+Health.++&volume=82&issue=6&pages=409-412&date=1991&au=Gallop,+Ruth+M. (georgetown.edu)
Syphilis2
Acquired Immunode3
- Estimates of the reduction in maternal-to-fetal transmission rates are taken from the AIDS [acquired immunodeficiency syndrome] Clinical Trials Group (ACTG) Protocol 076. (nih.gov)
- The development of sensitive and specific tests for antibody to human immunodeficiency virus type 1 (HIV-1) progressed rapidly after this retrovirus was identified as the cause of acquired immunodeficiency syndrome (AIDS). (cdc.gov)
- The Human Immunodeficiency Viruses type 1 and type 2 are etiological agents of the acquired immunodeficiency syndrome (AIDS). (rapidtest.com)
Prevention1
- AIDS Education & Prevention. (stacksdiscovery.com)
Dengue2
- This test will aid serodiagnosis, serosurveillance, and monitoring of Zika complications in dengue-endemic regions. (medscape.com)
- CDC researchers have identified and characterized discrete flavivirus cross-reactive epitopes useful for improving serodiagnosis and vaccination of flaviviruses, such as dengue virus, yellow fever virus, Japanese encephalitis virus, West Nile virus and the tick-borne encephalitis viruses. (nih.gov)
Sensitivity1
- In addition to, in VL/aids coinfected sufferers, decrease sensitivity was achieved in contrast with VL sufferers. (deepbloodbankequipments.com)
Discharge1
- Patterns of Prescribing Antiretroviral Therapy Upon Discharge to Psychiatry Inpatients With HIV/AIDS at a Large Urban Hospital. (ucsf.edu)
COMPLEX1
- HIV has been isolated from patients with AIDS, AIDS related complex (ARC) and from healthy individuals at high risk for (AIDS). (rapidtest.com)
Program1
- Reported by: Association of State and Territorial Public Health Laboratory Directors and AIDS Program, Center for Infectious Diseases, Public Health Practice Program Office, Centers for Disease Control* The Association of State and Territorial Public Health Laboratory Directors (ASTPHLD) and CDC have collaborated in preparing this report. (cdc.gov)
Variety1
- This CDC-developed invention pertains to multivalent antigenic peptides (MAPs) that can be used in a variety of HIV/AIDS diagnostics. (nih.gov)
Research1
- Geography Should Not Be Destiny: Focusing HIV/AIDS Implementation Research and Programs on Microepidemics in US Neighborhoods. (stacksdiscovery.com)
People1
- It is estimated that more than 300,000 people are infected each year, which is 7 times more prevalent than AIDS. (webs.com)
Life1
- The long dying of Kimberly Bergalis tapped strong emotional currents in the life of Americans and the AIDS epidemic. (georgetown.edu)