Agrobacterium tumefaciens: A species of gram-negative, aerobic bacteria isolated from soil and the stems, leafs, and roots of plants. Some biotypes are pathogenic and cause the formation of PLANT TUMORS in a wide variety of higher plants. The species is a major research tool in biotechnology.Rhizobium: A genus of gram-negative, aerobic, rod-shaped bacteria that activate PLANT ROOT NODULATION in leguminous plants. Members of this genus are nitrogen-fixing and common soil inhabitants.Agrobacterium: A genus of gram negative, aerobic, rod-shaped bacteria found in soil, plants, and marine mud.Plant Tumors: A localized proliferation of plant tissue forming a swelling or outgrowth, commonly with a characteristic shape and unlike any organ of the normal plant. Plant tumors or galls usually form in response to the action of a pathogen or a pest. (Holliday, P., A Dictionary of Plant Pathology, 1989, p330)Plant Tumor-Inducing Plasmids: Plasmids coding for proteins which induce PLANT TUMORS. The most notable example of a plant tumor inducing plasmid is the Ti plasmid found associated with AGROBACTERIUM TUMEFACIENS.Transformation, Genetic: Change brought about to an organisms genetic composition by unidirectional transfer (TRANSFECTION; TRANSDUCTION, GENETIC; CONJUGATION, GENETIC, etc.) and incorporation of foreign DNA into prokaryotic or eukaryotic cells by recombination of part or all of that DNA into the cell's genome.Bacterial Proteins: Proteins found in any species of bacterium.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Virulence Factors: Those components of an organism that determine its capacity to cause disease but are not required for its viability per se. Two classes have been characterized: TOXINS, BIOLOGICAL and surface adhesion molecules that effect the ability of the microorganism to invade and colonize a host. (From Davis et al., Microbiology, 4th ed. p486)Plasmids: Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.Plants: Multicellular, eukaryotic life forms of kingdom Plantae (sensu lato), comprising the VIRIDIPLANTAE; RHODOPHYTA; and GLAUCOPHYTA; all of which acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations.Genes, Bacterial: The functional hereditary units of BACTERIA.Kalanchoe: A plant genus of the family CRASSULACEAE. Members contain bryophyllins (also called bryotoxins) which are bufadienolides (BUFANOLIDES) that have insecticidal activity.Plants, Genetically Modified: PLANTS, or their progeny, whose GENOME has been altered by GENETIC ENGINEERING.Rhizobiaceae: A family of gram-negative bacteria which are saprophytes, symbionts, or plant pathogens.Tobacco: A plant genus of the family SOLANACEAE. Members contain NICOTINE and other biologically active chemicals; its dried leaves are used for SMOKING.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Zeatin: An aminopurine factor in plant extracts that induces cell division. (Grant & Hackh's Chemical Dict, 5th ed)Virulence: The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.Plants, Toxic: Plants or plant parts which are harmful to man or other animals.Gene Expression Regulation, Bacterial: Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.Conjugation, Genetic: A parasexual process in BACTERIA; ALGAE; FUNGI; and ciliate EUKARYOTA for achieving exchange of chromosome material during fusion of two cells. In bacteria, this is a uni-directional transfer of genetic material; in protozoa it is a bi-directional exchange. In algae and fungi, it is a form of sexual reproduction, with the union of male and female gametes.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Plant Diseases: Diseases of plants.4-Butyrolactone: One of the FURANS with a carbonyl thereby forming a cyclic lactone. It is an endogenous compound made from gamma-aminobutyrate and is the precursor of gamma-hydroxybutyrate. It is also used as a pharmacological agent and solvent.Genetic Complementation Test: A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.AcetophenonesAmino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Plant Roots: The usually underground portions of a plant that serve as support, store food, and through which water and mineral nutrients enter the plant. (From American Heritage Dictionary, 1982; Concise Dictionary of Biology, 1990)beta-Glucosidase: An exocellulase with specificity for a variety of beta-D-glycoside substrates. It catalyzes the hydrolysis of terminal non-reducing residues in beta-D-glucosides with release of GLUCOSE.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Chromosomes, Bacterial: Structures within the nucleus of bacterial cells consisting of or containing DNA, which carry genetic information essential to the cell.Sinorhizobium meliloti: A species of gram-negative, aerobic bacteria that causes formation of root nodules on some, but not all, types of sweet clover, MEDICAGO SATIVA, and fenugreek.Mutagenesis, Insertional: Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene or extragenic sequence. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA insertions into or adjacent to a cellular proto-oncogene can interrupt GENETIC TRANSLATION of the coding sequences or interfere with recognition of regulatory elements and cause unregulated expression of the proto-oncogene resulting in tumor formation.Arginine: An essential amino acid that is physiologically active in the L-form.Operon: In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.DNA Transposable Elements: Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.Datura stramonium: A plant species of the genus DATURA, family SOLANACEAE, that contains TROPANES and other SOLANACEOUS ALKALOIDS.Hygromycin B: Aminoglycoside produced by Streptomyces hygroscopicus. It is used as an anthelmintic against swine infections by large roundworms, nodular worms, and whipworms.GlucuronidasePlant Somatic Embryogenesis Techniques: The process of embryo initiation in culture from vegetative, non-gametic, sporophytic, or somatic plant cells.Cytokinins: Plant hormones that promote the separation of daughter cells after mitotic division of a parent cell. Frequently they are purine derivatives.Plant Leaves: Expanded structures, usually green, of vascular plants, characteristically consisting of a bladelike expansion attached to a stem, and functioning as the principal organ of photosynthesis and transpiration. (American Heritage Dictionary, 2d ed)Sequence Homology, Amino Acid: The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.beta-Glucans: Glucose polymers consisting of a backbone of beta(1->3)-linked beta-D-glucopyranosyl units with beta(1->6) linked side chains of various lengths. They are a major component of the CELL WALL of organisms and of soluble DIETARY FIBER.Brucella suis: A species of gram-negative bacteria, primarily infecting SWINE, but it can also infect humans, DOGS, and HARES.DNA, Single-Stranded: A single chain of deoxyribonucleotides that occurs in some bacteria and viruses. It usually exists as a covalently closed circle.Fabaceae: The large family of plants characterized by pods. Some are edible and some cause LATHYRISM or FAVISM and other forms of poisoning. Other species yield useful materials like gums from ACACIA and various LECTINS like PHYTOHEMAGGLUTININS from PHASEOLUS. Many of them harbor NITROGEN FIXATION bacteria on their roots. Many but not all species of "beans" belong to this family.Arabidopsis: A plant genus of the family BRASSICACEAE that contains ARABIDOPSIS PROTEINS and MADS DOMAIN PROTEINS. The species A. thaliana is used for experiments in classical plant genetics as well as molecular genetic studies in plant physiology, biochemistry, and development.Phosphoric Triester Hydrolases: A class of enzymes that catalyze the hydrolysis of one of the three ester bonds in a phosphotriester-containing compound.Species Specificity: The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.Mannitol: A diuretic and renal diagnostic aid related to sorbitol. It has little significant energy value as it is largely eliminated from the body before any metabolism can take place. It can be used to treat oliguria associated with kidney failure or other manifestations of inadequate renal function and has been used for determination of glomerular filtration rate. Mannitol is also commonly used as a research tool in cell biological studies, usually to control osmolarity.Quorum Sensing: A phenomenon where microorganisms communicate and coordinate their behavior by the accumulation of signaling molecules. A reaction occurs when a substance accumulates to a sufficient concentration. This is most commonly seen in bacteria.Genetic Vectors: DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.Protocatechuate-3,4-Dioxygenase: An enzyme that catalyzes the oxidation of protocatechuate to 3-carboxy-cis-cis-muconate in the presence of molecular oxygen. It contains ferric ion. EC 1.13.11.3.Glucans: Polysaccharides composed of repeating glucose units. They can consist of branched or unbranched chains in any linkages.Alphaproteobacteria: A class in the phylum PROTEOBACTERIA comprised mostly of two major phenotypes: purple non-sulfur bacteria and aerobic bacteriochlorophyll-containing bacteria.Transformation, Bacterial: The heritable modification of the properties of a competent bacterium by naked DNA from another source. The uptake of naked DNA is a naturally occuring phenomenon in some bacteria. It is often used as a GENE TRANSFER TECHNIQUE.Sequence Homology, Nucleic Acid: The sequential correspondence of nucleotides in one nucleic acid molecule with those of another nucleic acid molecule. Sequence homology is an indication of the genetic relatedness of different organisms and gene function.Gene Expression Regulation, Plant: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in plants.DNA-Binding Proteins: Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.DNA, Plant: Deoxyribonucleic acid that makes up the genetic material of plants.Periplasmic Binding Proteins: Periplasmic proteins that scavenge or sense diverse nutrients. In the bacterial environment they usually couple to transporters or chemotaxis receptors on the inner bacterial membrane.Acyl-Butyrolactones: Cyclic esters of acylated BUTYRIC ACID containing four carbons in the ring.Restriction Mapping: Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.Lycopersicon esculentum: A plant species of the family SOLANACEAE, native of South America, widely cultivated for their edible, fleshy, usually red fruit.Gene Transfer Techniques: The introduction of functional (usually cloned) GENES into cells. A variety of techniques and naturally occurring processes are used for the gene transfer such as cell hybridization, LIPOSOMES or microcell-mediated gene transfer, ELECTROPORATION, chromosome-mediated gene transfer, TRANSFECTION, and GENETIC TRANSDUCTION. Gene transfer may result in genetically transformed cells and individual organisms.Linaria: A plant genus of the family Plantaginaceae. Members contain linarin (also called acaciin).Plant Proteins: Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which VEGETABLE PROTEINS is available.Sequence Alignment: The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.Nitrogen Fixation: The process in certain BACTERIA; FUNGI; and CYANOBACTERIA converting free atmospheric NITROGEN to biologically usable forms of nitrogen, such as AMMONIA; NITRATES; and amino compounds.Genetic Engineering: Directed modification of the gene complement of a living organism by such techniques as altering the DNA, substituting genetic material by means of a virus, transplanting whole nuclei, transplanting cell hybrids, etc.Laccaria: A genus of white-spored mushrooms in the family Tricholomataceae. They form symbiotic partnerships (MYCORRHIZAE) with trees.Plants, Medicinal: Plants whose roots, leaves, seeds, bark, or other constituent parts possess therapeutic, tonic, purgative, curative or other pharmacologic attributes, when administered to man or animals.Chromobacterium: A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria occurring in soil and water. Its organisms are generally nonpathogenic, but some species do cause infections of mammals, including humans.Oxazines: Six-membered heterocycles containing an oxygen and a nitrogen.RNA, Bacterial: Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.Ion Channels: Gated, ion-selective glycoproteins that traverse membranes. The stimulus for ION CHANNEL GATING can be due to a variety of stimuli such as LIGANDS, a TRANSMEMBRANE POTENTIAL DIFFERENCE, mechanical deformation or through INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS.Replicon: Any DNA sequence capable of independent replication or a molecule that possesses a REPLICATION ORIGIN and which is therefore potentially capable of being replicated in a suitable cell. (Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)Ammonia-Lyases: Enzymes that catalyze the formation of a carbon-carbon double bond by the elimination of AMMONIA. EC 4.3.1.Phylogeny: The relationships of groups of organisms as reflected by their genetic makeup.Promoter Regions, Genetic: DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.Mutagenesis: Process of generating a genetic MUTATION. It may occur spontaneously or be induced by MUTAGENS.Soil Microbiology: The presence of bacteria, viruses, and fungi in the soil. This term is not restricted to pathogenic organisms.Nucleic Acid Hybridization: Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)Rhizobium leguminosarum: A species of gram-negative, aerobic bacteria that is found in soil and which causes formation of root nodules on some, but not all, types of field pea, lentil, kidney bean, and clover.Genome, Bacterial: The genetic complement of a BACTERIA as represented in its DNA.Symbiosis: The relationship between two different species of organisms that are interdependent; each gains benefits from the other or a relationship between different species where both of the organisms in question benefit from the presence of the other.DNA Restriction Enzymes: Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.Daucus carota: A plant species of the family APIACEAE that is widely cultivated for the edible yellow-orange root. The plant has finely divided leaves and flat clusters of small white flowers.Gossypium: A plant genus of the family MALVACEAE. It is the source of COTTON FIBER; COTTONSEED OIL, which is used for cooking, and GOSSYPOL. The economically important cotton crop is a major user of agricultural PESTICIDES.DNA Primers: Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.Hydroxybenzoates: Benzoate derivatives substituted by one or more hydroxy groups in any position on the benzene ring.Indoleacetic Acids: Acetic acid derivatives of the heterocyclic compound indole. (Merck Index, 11th ed)Medicago sativa: A plant species of the family FABACEAE widely cultivated for ANIMAL FEED.beta-Galactosidase: A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.Recombinant Fusion Proteins: Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.Open Reading Frames: A sequence of successive nucleotide triplets that are read as CODONS specifying AMINO ACIDS and begin with an INITIATOR CODON and end with a stop codon (CODON, TERMINATOR).Zea mays: A plant species of the family POACEAE. It is a tall grass grown for its EDIBLE GRAIN, corn, used as food and animal FODDER.Solanum tuberosum: A plant species of the genus SOLANUM, family SOLANACEAE. The starchy roots are used as food. SOLANINE is found in green parts.Culture Media: Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.Adipates: Derivatives of adipic acid. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain a 1,6-carboxy terminated aliphatic structure.CinnamatesPili, Sex: Filamentous or elongated proteinaceous structures which extend from the cell surface in gram-negative bacteria that contain certain types of conjugative plasmid. These pili are the organs associated with genetic transfer and have essential roles in conjugation. Normally, only one or a few pili occur on a given donor cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed, p675) This preferred use of "pili" refers to the sexual appendage, to be distinguished from bacterial fimbriae (FIMBRIAE, BACTERIAL), also known as common pili, which are usually concerned with adhesion.Phenotype: The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.GlutaratesPolygalacturonase: A cell wall-degrading enzyme found in microorganisms and higher plants. It catalyzes the random hydrolysis of 1,4-alpha-D-galactosiduronic linkages in pectate and other galacturonans. EC 3.2.1.15.

Performance and long-term stability of the barley hordothionin gene in multiple transgenic apple lines. (1/102)

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Gene knockdown by ihpRNA-triggering in the ectomycorrhizal basidiomycete fungus Laccaria bicolor. (2/102)

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Efficient Agrobacterium-based transient expression system for the production of biopharmaceuticals in plants. (3/102)

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Agrobacterium-mediated transformation of kabocha squash (Cucurbita moschata Duch) induced by wounding with aluminum borate whiskers. (4/102)

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Transformed hairy roots of Discaria trinervis: a valuable tool for studying actinorhizal symbiosis in the context of intercellular infection. (5/102)

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Characterization and crop production efficiency of diazotrophic bacterial isolates from coastal saline soils. (6/102)

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A mobile signal transported over a long distance induces systemic transcriptional gene silencing in a grafted partner. (7/102)

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FVE, an Arabidopsis homologue of the retinoblastoma-associated protein that regulates flowering time and cold response, binds to chromatin as a large multiprotein complex. (8/102)

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TY - JOUR. T1 - Agrobacterium rhizogenes-mediated transformation. T2 - Root cultures as a source of alkaloids. AU - Sevon, Nina. AU - Oksman-Caldentey, Kirsi-Marja. PY - 2002. Y1 - 2002. N2 - Hairy roots, transformed with Agrobacterium rhizogenes, have been found to be suitable for the production of secondary metabolites because of their stable and high productivity in hormone-free culture conditions. A number of plant species including many medicinal plants have been successfully transformed with Agrobacterium rhizogenes. Transformed root cultures have also been found to be a potential source of high-value pharmaceuticals. In this article the most important alkaloids produced by hairy roots are summarised. Several different methods have been used to increase the alkaloid accumulation in hairy root cultures. The selection of high productive root lines based on somaclonal variation offers an interesting option to enhance the productivity. Elicitors and modification of culture conditions have been ...
Background Transient gene expression via Agrobacterium-mediated DNA transfer offers a simple and fast method to analyze transgene functions. Although Arabidopsis is the most-studied model plant with powerful genetic and genomic resources, achieving highly efficient and consistent transient expression for gene function analysis in Arabidopsis remains challenging. Results We developed a highly efficient and robust Agrobacterium-mediated transient expression system, named AGROBEST (Agrobacterium-mediated enhanced seedling transformation), which achieves versatile analysis of diverse gene functions in intact Arabidopsis seedlings. Using β-glucuronidase (GUS) as a reporter for Agrobacterium-mediated transformation assay, we show that the use of a specific disarmed Agrobacterium strain with vir gene pre-induction resulted in homogenous GUS staining in cotyledons of young Arabidopsis seedlings. Optimization with AB salts in plant culture medium buffered with acidic pH 5.5 during Agrobacterium infection
TY - JOUR. T1 - Hairy-root organ cultures for the production of human acetylcholinesterase. AU - Woods, Ryan R.. AU - Geyer, Brian C.. AU - Leket-Mor, Tsafrir. PY - 2008/12/23. Y1 - 2008/12/23. N2 - Background: Human cholinesterases can be used as a bioscavenger of organophosphate toxins used as pesticides and chemical warfare nerve agents. The practicality of this approach depends on the availability of the human enzymes, but because of inherent supply and regulatory constraints, a suitable production system is yet to be identified. Results: As a promising alternative, we report the creation of "hairy root" organ cultures derived via Agrobacterium rhizogenes-mediated transformation from human acetylcholinesterase-expressing transgenic Nicotiana benthamiana plants. Acetylcholinesterase-expressing hairy root cultures had a slower growth rate, reached to the stationary phase faster and grew to lower maximal densities as compared to wild type control cultures. Acetylcholinesterase accumulated to ...
See on Scoop.it - Virology and Bioinformatics from Virology.caThe flagellotropic phage 7-7-1 infects motile cells of Agrobacterium sp H13-3 by attaching to and traveling along the rotating flagellar filament to the secondary receptor at the base, where it injects its DNA into the host cell. This is an interesting paper, because it describes a…
Sorghum is the fifth most widely planted cereal crop in the world and is commonly cultivated in arid and semi-arid regions such as Africa. Despite its importance as a food source, sorghum genetic improvement through transgenic approaches has been limited because of an inefficient transformation system. Here we report a ternary vector (also known as co-habitating vector) system using a recently described pVIR accessory plasmid that facilitates efficient Agrobacterium-mediated transformation of sorghum. We report regeneration frequencies ranging from 6-29% in Tx430 using different selectable markers and single copy, backbone free "quality events" ranging from 45-66% of the total events produced ...
We have established an efficient Agrobacterium-mediated transformation procedure for Arabidopsis thaliana genotype C24 using the chimeric bialaphos resistance gene (bar) coding for phosphinothricin ac
The exploration of space is one of the most inspiring areas of scientific research and a major driver of technological innovation. One of the major factors…
There are a lot of good conferences coming up this year, some small and some very big; some free and some rather more expensive. If you are put off by registration or accommodation fees, you dont have to be. There are grants available for people to travel to conferences and workshops, and Ive listed some below. I havent mentioned grants for specific conferences, but most learned societies offer grants for students and new post-docs to attend their events. Please let me know in the comments or via email (charis @ garnetcommunity.org.uk) or Twitter if you know of any general funds which I havent listed.. Not that Im biased (of course…) but the GARNet workshop on Plant Synthetic Biology and UK PlantSci 2013 are excellent opportunities to use these funds! Plant Synthetic Biology registration starts from £175 for students, including accommodation, lunches, and conference dinner. Early bird registration for PlantSci 2013 starts from £80 for students, including lunch and refreshments, but you ...
We have identified quantitative trait loci (QTL) for transgenic and adventitious root production using an Agrobacterium rhizogenes-mediated co-transformati
Transgenic tomato plants of south Indian cultivar Arka Vikas were developed using Agrobacterium strain EHA 105, harbouring Bt Cry2A gene with a construct containing 35S CaMV promoter, OCS terminator and nptII selectable marker, through Agrobacterium-mediated transformation. This study was conducted to improve the regeneration and transformation protocol for south Indian cultivar Arka Vikas. Hypocotyl was used as explant source for transformation due to high regeneration efficiency, molecular analysis through PCR for putative transformants in T0 generation and qualitative ELISA method was performed for Bt protein expression followed by insect bioassays. Insect bioassay studies was conducted using neonate larva of helicoverpa armigera to screen the plants and the plants expressing good resistance with molecular and phenotypic characters were carried further for successive generations. The experimental results concluded that Bt gene was deployed in tomato cultivar successfully and had developed resistance
The Harvard iGarden is a venture into plant engineering. Our aim is to create a toolkit for the cultivation of a personalized garden containing features introduced through synthetic biology. We are currently working on two independent features to be included in this toolkit - inclusion of novel flavors and knockdown of plant allergens. In addition, we are working on the creation of a "genetic fence" to prevent the spread of foreign genetic material. All components are designed to fit the biobrick standard.. We are introducing biobrick parts to plants through agrobacterium-mediated transformation. To do so, we have modified existing agrobacterium vectors designed for plant transformation to include the biobrick multiple cloning site.. The Harvard iGarden, beyond being a simple application of the biobrick system to plant engineering, is an effort to raise public awareness of synthetic biology by bringing its effects down to a personal and more tangible level. We envision the iGarden as an ...
OpenPlant: sharing tools for a sustainable future. OpenPlant is a joint initiative in plant synthetic biology between the University of Cambridge, John Innes Centre and the Earlham Institute, funded by the BBSRC and EPSRC as part of the UK Synthetic Biology for Growth programme, directed in Cambridge by Prof. Jim Haseloff and in Norwich by Prof. Anne Osbourn.
OpenPlant: sharing tools for a sustainable future. OpenPlant is a joint initiative in plant synthetic biology between the University of Cambridge, John Innes Centre and the Earlham Institute, funded by the BBSRC and EPSRC as part of the UK Synthetic Biology for Growth programme, directed in Cambridge by Prof. Jim Haseloff and in Norwich by Prof. Anne Osbourn.
A World Leader in Plant Synthetic Biology: Understanding Plants and Using Our Plant Knowledge for Human and Environmental Use ...
Standardized DNA assembly strategies facilitate the generation of multigene constructs from collections of building blocks in plant synthetic biology. A common syntax for hierarchical DNA assembly following the Golden Gate principle employing Type IIs restriction endonucleases was recently developed, and underlies the Modular Cloning and GoldenBraid systems. In these systems, transcriptional units and/or multigene constructs are assembled from libraries of standardized building blocks, also referred to as phytobricks, in several hierarchical levels and by iterative Golden Gate reactions. This combinatorial assembly strategy meets the increasingly complex demands in biotechnology and bioengineering, and also represents a cost-efficient and versatile alternative to previous molecular cloning techniques. For Modular Cloning, a collection of commonly used Plant Parts was previously released together with the Modular Cloning toolkit itself, which largely facilitated the adoption of this cloning ...
Bhatia, R, Dalton, SJ, Roberts, LA, Gallagher, JA & Bosch, M 2014, Genetic engineering of lignocellulosic biomass in grasses: from gene function analysis to targeted in planta deconstruction, First International Workshop on Plant Synthetic Biology, Boston, United States of America, 17 May 2014 - 18 May 2014 ...
Agrobacterium-mediated transformation: Agrobacterium-mediated transformation has been used to create commercial cultivars for over 10 years and is known to create insertion-site mutations (Table 2, Section 1.1). However, there has been only one large-scale study of the mutations created at insertion events2 containing single T-DNA3inserts (the type of event preferred for commercial purposes; Forsbach et al. 2003). In this study of 112 single-copy T-DNA insertion events in A. thaliana, the researchers found that exact T-DNA integration almost never occurred (Forsbach et al. 2003). Most of the T-DNA insertions resulted in small (1-100 base pair) deletions of plant genomic sequences at the insertion-site. However, for a significant number (24/112) there was evidence for large-scale rearrangement of plant genomic DNA at the insertion-site. Two of these insertion events contained chromosomal translocations. The rest had rearrangements which were not fully characterised. It is known, however, that ...
5CASD3.xxxx-00 SDL3/SDL4 cables are designed to transfer SDL3/SDL4 data and enable easy cable installation. Due to the RJ45 connector, the cable is also suitable for narrow feed-throughs, e.g. in swing arm shafts.. ...
Profiles of Sina (upper line) and Sinb (lower line) gene expression in developing spikes of the non-transgenic plants of cv. Wanad from 8th to 32nd day after po
The following protocol is designed for NEB 10-beta Competent E. coli (NEB #C3019 ) which are included in the NEB PCR Cloning Kit (NEB #E1202 ) only
There is a broad range of methods that are employed in the process of producing GMOs. This often involves insertion of a gene of interest into living organisms depending on the species that you are working on. In plants mainly, two most common biotechnology-based techniques include; Agrobacterium-mediated transformation and bombardment of particles. According to the regulations given by FSANZ, it is a requirement that clear description of the method employed in genetically engineering plants is given.. Case study- Roundup Ready soy. This was produced using the particle bombardment method. This process of biotechnologically engineering soybeans involved; bombardment of the plant cells with microscopic particles of gold coated with DNA that contains the gene of interest. The gene of interest is the EPSPS gene that is derived from Agrobacterium. The aim of this is to introduce the novel gene of interest through the cell wall so that it integrates into the genetic material of the soy plant. The new ...
USING YEAST AS MODEL, A SCREENING WAS PERFORMED AND MON2 WAS IDENTIFIED AS A NOVEL HOST FACTOR THAT WAS INVOLVED IN AGROBACTERIUM-MEDIATED TRANSFORMATION (AMT) OF YEAST. FURTHER STUDIES HAVE REVEALED THAT MON2 MEDIATES TRANSIENT AMT NEGATIVELY. WHILE AT THE SAME TIME PLAY A POSITIVE ROLE IN MEDIATING STABLE AMT ...
On April 12, CASHS students in the autism support program hosted their younger counterparts from Hamilton Heights Elementary School: Cooking and serving lunch to them, entertaining them at a choral concert, reading to them and helping them put their hand prints on an Autism Awareness banner.
SEQUENCE 797 AA; 91680 MW; 28EA7CA7A1DE223C CRC64; MAALAYNLGK REINHYFSVR SAKVLALVAV LLLAACHLAS RRYRGNDSCE. YLLSSGRFLG EKVWQPHSCM MHKYKISEAK NCLVDKHIAF IGDSRIRQLF. YSFVKIINPQ FKEEGNKHEN IPFEDKTASV KVDFLWHPEV NGSMKQCIKV. WTEDSIAKPH VIVAGAATWS IKIHNGSSEA LSQYKMNITS IAPLLEKLAK. TSDVYWVLQD PVYEDLLSEN RKMITNEKID AYNEAAVSIL NSSTRNSKSN. VKMFSVSKLI AQETIMESLD GLHLPESSRE TTAMILMNVY CNKILKPVDG SCCQPRPPVT LIQKLAACFF TLSIIGYLIF YIIHRNAHRK NKPCTDLESG. EEKKNIINTP VSSLEILLQS FCKLGLIMAY FYMCDRANLF MKENKFYTHS. SFFIPIIYIL VLGVFYNENT KETKVLNREQ TDEWKGWMQL VILIYHISGA. STFLPVYMHI RVLVAAYLFQ TGYGHFSYFW IKGDFGIYRV CQVLFRLNFL. VVVLCIVMDR PYQFYYFVPL VTVWFMVIYV TLALWPQIIQ KKANGNCFWH. FGLLLKLGFL LLFICFLAYS QGAFEKIFSL WPLSKCFELK GNVYEWWFRW RLDRYVVFHG MLFAFIYLAL QKRQILSEGK GEPLFSNKIS NFLLFISVVS. FLTYSIWASS CKNKAECNEL HPSVSVVQIL AFILIRNIPG YARSVYSSFF. AWFGKISLEL FICQYHIWLA ADTRGILVLI PGNPMLNIIV STFIFVCVAH. EISQITNDLA QIIIPKDNSS LLKRLACIAA FFCGLLILSS IQDKSKH ...
reproduction: revisions to the UC Davis Powdery Mildew Risk Index. PLANT DISEASE 97(11): 1438-1447. ... F, Marchi, G, Surico, G. (2010). Indexing Agrobacterium vitis in asymptomatic grapevine .... ...
reproduction: revisions to the UC Davis Powdery Mildew Risk Index. PLANT DISEASE 97(11): 1438-1447. ... F, Marchi, G, Surico, G. (2010). Indexing Agrobacterium vitis in asymptomatic grapevine .... ...
Za vse karantenske kodljive organizme bo tudi v prihodnje potrebno v Sloveniji zagotoviti temeljit nadzor, za karantenske kodljive organizme s seznama A1, ki so bili v Sloveniji odkriti v zadnjem asu, pa se bo treba opredeliti tudi do njihovega prihodnjega statusa. Tudi na splo no bo potrebno status karantenskih kodljivih organizmov na slovenskih karantenskih listah ponovno preveriti in jih uskladiti z Aneksom I Smernice 77/93/EC.. Portoro , 4. marca 1999. Poro ilo pripravila predsedujo a sekcije Jo i Jerman Cvelbar. Sekcija »Varstvo vinske trte«. Sekcija je obravnavala aktualno problematiko in novej a spoznanja na podro ju varstva vinske trte, diagnostike nekaterih problemati nih bolezni, zlasti raka vinske trte. S stali a prispevka k znanosti je potrebno posebej izpostaviti referata S. Vrabla o okolju prijazni metodi zbeganja pri zatiranju grozdnih suka ev ter M. abec o raku vinske trte (Agrobacterium vitis). Portoro , 4. marca 1999. Poro ilo pripravil predsedujo i sekcije dr. Stojan ...
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Synthetic biology is producing a paradigm shift in biotechnology based on the introduction of engineering principles in the design of new organisms by genetic modification (Check, 2005; Haseloff and Ajioka, 2009). Whereas synthetic biology has rapidly permeated microbial biotechnology, the engineering of multicelled organisms following synthetic biology principles is now emerging and is mainly driven by the so-called top-down approaches, where newly engineered genetic circuits are embedded into naturally existing organisms used as a "chassis." The plant chassis offers an extraordinarily fertile ground for synthetic biology-like engineering. However, technology still faces the huge challenge of performing engineering-driven genetic designs. One of the main technological challenges of plant synthetic biology requires the construction and transfer of multigene structures to the plant genome. This is putting pressure on developing DNA assembly and transformation technologies adapted to plants. One ...
Recent studies on the root proteome of Medicago truncatula (Gaertn.) showed an induction of pathogenesis-related (PR) proteins of the class 10 after infection with the oomycete pathogen Aphanomyces euteiches (Drechs.). To get insights into the function of these proteins during the parasitic root-microbe association, a gene knockdown approach using RNAi was carried out. Agrobacterium rhizogenes-mediated transformation of M. truncatula roots led to a knockdown of the Medicago PR10-1 gene in transgenic in vitro root cultures. Proteomic analyses of the MtPr10-1i root cultures showed that MtPr10-1 was efficiently knocked down in two MtPr10-1i lines. Moreover, five additional PR-10-type proteins annotated as abscisic acid responsive proteins (ABR17s) revealed also an almost complete silencing in these two lines. Inoculation of the root cultures with the oomycete root pathogen A. euteiches resulted in a clearly reduced colonization and thus in a suppressed infection development in MtPr10-1i roots as ...
Sarker, R.H. and Biswas, A. (2002) In Vitro Plantlet Regeneration and Agrobacterium-Mediated Genetic Transformation of Wheat (Triticum aestivum L.) Plant Tissue Culture, 12, 155-165.
Read "Sonication-assisted Agrobacterium-mediated transformation of the ACC gene to interfere the production of ethylene in spring Dendrobium cv.
Mungbean is an important pulse crop extensively cultivated in Southeast Asia for supply of easily digestible protein. Salinity severely limits the growth and productivity of mungbean, and weeding poses nutritional and disease constraints to mungbean cultivation. To pyramid both salt tolerance and protection against herbicide in mungbean, the AtNHX1 encoding tonoplast Na+/H+ antiporter from Arabidopsis, and bar gene associated with herbicide resistance were co-expressed through Agrobacterium-mediated transformation. Stress inducible expression of AtNHX1 significantly improved tolerance under salt stress to ionic, osmotic and oxidative stresses in transgenic mungbean plants compared to the wild type (WT) plants, whereas constitutive expression of bar provided resistance to herbicide. Compared to WT, transgenic mungbean plants grew better with higher plant height, foliage, dry mass and seed yield under high salt stress (200 mM NaCl) in the greenhouse. The improved performance of transgenic plants under
The study was carried out to evaluate the amenability of tropical inbred and hybrid maize lines, using Agrobacterium mediated transformation technique. Agrobacteriumtumefaciens strains EHA101 harbouri
The diguanylate cyclase from Agrobacterium vitis has been engineered to remove phosphodiesterase activity, allowing for production of cyclic-diGMP from guanosine triphosp
In an effort to engineer plants to be resistant to glyphosate, scientists starting looking for glyphosate resistant EPSP in the 1980s. Several methods were employed including selection, directed evolution, site-directed mutagenesis, and microbial screens. It was difficult to obtain a suitable enzyme because typically the resistant enzymes had an undesirable decrease in catalytic activity. Eventually, naturally occurring glyphosate-tolerant microbes were identified including Agrobacterium sp. Strain CP4, Achromobacter sp. Strain LBAA, and Pseudomonas sp. Strain PG2982. The enzymes isolate from these maintained good catalyctic ativity while being resistant to glyphosate (Funke). Agrobacterium sp. Strain CP4 was obtained from a glyphosate rich waste area at a glyphosate production facility. There is substantial sequence variation between these resistant enzymes and those of plants or E. coli. Other versions resistant versions of EPSP have been identified in Streptococcus pneumonia and ...
Bar gene coding Glufosinate resistance is transformed into the alfalfa variety, Caoyuan No.1, mediated by Agrobacterium tumefaciens. The leaves of transgenic plants after being screened and tested are proved to be resistant to Glufosinate. Furthermore, PCR analysis shows that bar gene has been transformed into Alfalfa.
The aim of the proposed project (in collaboration with Geneticlab S.r.l.) is to design reliable protocols to produce biomass and to induce lipid accumulation in cell cultures obtained from a plant species known as high oleic: Jatropha curcas. This species has attracted the interest of various developmental agencies in the tropics and subtropics due to its easy adaptability to semi-arid marginal sites, use of the oil as a diesel fuel substitute and its use in erosion control. J. curcas is monoecious, with male and female flowers on the same plant, and its center of origin are Mexico and Central America. In its natural area of distribution, the species is most abundant in tropical savanna and monsoon climates and in temperate climates without a dry season and with a hot summer ...
Hello researchers, , ,I am study one gene promoter and fused it to GUS in pCAMBIA1391Z, in which ,CaMV35S drives the selectable marker Hyg gene. Among the 10 independent T3 ,lines, which were confirmed by PCR, some show very strong GUS staining, ,some no, and some weak staining. , ,I knew from this paper that vectors containing 35S promoter driving ,selectable marker gene are not good for promoter study. ,The 35S promoter used in a selectable marker gene of a plant transformation ,vector affects the expression of the transgene. Planta. Volume 221, Number ,4 / June, 2005 , ,But I found that quite many published papers used vectors (like ,pCAMBIA1391, pCAMBIA1301, etc) containing 35S promoter driving selectable ,marker. , ,As the staining patterns of my promoter fusion lines are not consistent at ,al, I want to use another vector pBI121 or pCGN1547. Does anyone know where ,to get pCGN1547? ,How do you deal with those lines from 35S containing vector? CAMBIA ,suggests doing co-transformation ...
is provided, focused on the role played by the different components of the virulence system. The general assessments for the establishment of efficient transformation protocols are discussed with an emphasis in the application of this methodology to monocotyledonous plants. Based on our own experience, we present the establishment of sugarcane transformation by A. tumefaciens as a model of application of this methodology to an important culture plant species, previously considered recalcitrant and inaccessible for this type of genetic manipulation ...
Agrobacterium-medieret transformation ved hjælp af en blomster-dip metode kan anvendes med succes at skabe stabile transgene linjer af...
Triplex End-Point PCR kit. "The major symptoms of infection by Agrobacterium vitis are galls found on the lower trunk, near the soil line, but galls can also develop slightly below the soil surface, or extend up to 1-m height. Infected plants produce inferior shoots, and portions of the vine above the gall may die. A. vitis mainly enters the plant through wounds caused by freezing, so climatic conditions favouring freeze injury also favour infection by A. vitis. Contaminated planting material can, however, also be a source of inoculum.". Source: EPPO Bulletin 32, 367-369. ...
Agrobacterium vitis genes for 16S rRNA, tRNA-Ile, tRNA-Ala, 23S rRNA 150-b fragment, IVS, 23S rRNA 1.3-kb fragment A, partial and complete sequence, strain: NCPPB-1771, locus: ...
A enzima mio-inositol-1-fosfato sintase (MIPS1) (E.C.5.5.1.4) catalisa a conversão irreversível de D-Glicose-6-P para 1-L-mio-inositol-1-P. O mio-inositol desempenha papel de destaque no metabolismo vegetal, fornecendo inositol e inositídeos em processos metabólicos essenciais à formação do vegetal. Dentre as várias vias nas quais o mio-inositol está envolvido algumas são alvo do melhoramento vegetal, a exemplo a manipulação do conteúdo de ácido fítico e de oligossacarídeos em sementes e açúcares estruturais na parede celular. Até o presente momento, plantas MIPS silenciadas não foram caracterizadas em detalhes, principalmente em suas estruturas vegetativas. Com isso, o presente trabalho objetivou caracterizar tomateiros Moneymaker com diferentes níveis de silenciamento de MIPS1. Para a indução do silenciamento utilizou- se tranformação genética via Agrobacterium tumefaciens contendo plasmídeo pCambia com construção tipo intron hairpin de fragmentos do gene MIPS de ...
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Co-cultivation of production cells at the limit of the in vitro age is an assay we offer to test for latent retroviruses for our clients. Essentially, your production cells are plated along with susceptible cell lines that exhibit stronger phenotypic CPE when the virus enters them. Thus, viruses that are expressed as non-infectious particles or exhibit low level of expression in production cells are detected ...
Co-cultivation of production cells at the limit of the in vitro age is an assay we offer to test for latent retroviruses for our clients. Essentially, your production cells are plated along with susceptible cell lines that exhibit stronger phenotypic CPE when the virus enters them. Thus, viruses that are expressed as non-infectious particles or exhibit low level of expression in production cells are detected ...
Graduates of Bu-Ali Sina University - the names, photos, skill, job, location. Information on the Bu-Ali Sina University - contacts, students, faculty, finances.
一基本信息姓名:周虚出生年月:1965年8月15日生职称:吉林大学动物科学学院教授、博导联系方式:0431-87835142,Email:[email protected]招生学科:动物遗传育种与繁殖研究方向:动物生殖调控二研究特色1.牛、猪卵泡和早期胚胎发育调控的分子机制2.营养对母猪繁殖的影响及其内分泌和分子机制3.猪繁殖障碍检测与防治三学术任职教育部高校教学指导委员会委员中国奶业协会繁殖专业委员会副主任中国畜牧兽医学会动物繁殖分会常务理事、副秘书长吉林省畜牧兽医学会常务理事四学历和学位1981
"Rhizobium/Agrobacterium group". National Center for Biotechnology Information (NCBI) taxonomy database. Retrieved 2012-05-02. ...
Ophel, K; Kerr, A (1990). "Agrobacterium vitis sp. nov. for strains of Agrobacterium biovar 3 from grapevines". Int J Syst Evol ... "Organism Overview: Agrobacterium vitis". National Center for Biotechnology Information. U.S. National Library of Medicine. 28 ... A 1999 study suggested that A. vitis is more closely related to Rhizobium galegae than to other Agrobacterium species, based on ... "Crown gall (Agrobacterium vitis)". Government of British Columbia. British Columbia Ministry of Agriculture. January 2009. ...
Sukanya NK, Vaidyanathan CS (1964). "Aminotransferases of Agrobacterium tumefaciens. Transamination between tryptophan and ...
Agrobacterium rhizogenes insert T-DNA into the genome of the host plant root cells. nature. 1982; 295:432-4 Cardarelli M, ... Rhizobium rhizogenes (formerly Agrobacterium rhizogenes) is a Gram-negative soil bacterium that produces hairy root disease in ... "Agrobacterium rhizogenes T-DNA genes capable of inducing hairy root phenotype". Molecular and General Genetics 209(3):475-480. ... Intrieri, M. C. & Buiatti, M. (July 2001). "The horizontal transfer of Agrobacterium rhizogenes genes and the evolution of the ...
These include the following: Aeromonadaceae, Vibrionaceae and related organisms; Genera Agrobacterium and Rhizobium; Bacillus ...
myxogenes) cyclosophorans (Agrobacterium spp., Rhizobium spp. and Xanthomonas spp.) dextran (Leuconostoc mesenteroides, ... Agrobacterium radiobacter, Pseudomonas marginalis, Rhizobium spp. and Zooglea' spp.) galactosaminogalactan (Aspergillus spp.) ...
Main article: Agrobacterium. Based on molecular data it was shown that the genus Agrobacterium is nested in Rhizobium and the ... Given the plant pathogenic nature of Agrobacterium species, it was proposed to maintain the genus Agrobacterium[95] and the ... Farrand, S.; Van Berkum, P.; Oger, P. (2003). "Agrobacterium is a definable genus of the family Rhizobiaceae". International ... Young, J.; Kuykendall, L.; Martínez-Romero, E.; Kerr, A.; Sawada, H. (2003). "Classification and nomenclature of Agrobacterium ...
Agrobacterium tumefaciens-mediated plant transformation. MON 88017 is an Insect resistant maize expressing the Cry3Bb1 protein ...
Van Keer C, Kersters K, De Ley J (September 1976). "L-Sorbose metabolism in Agrobacterium tumefaciens". Antonie Van Leeuwenhoek ...
One example is the Agrobacterium sp. β-glucosythase, which forms a β 1-4 glycoside with Glucose as the acceptor, but forms a β ... The first reported glycosynthase was a mutant of the Agrobacterium sp. β-glucosidase / galactosidase in which the nucleophile ...
... "queen of Agrobacterium." Eileen Gail de Planque, Ph.D. (1944-2010), expert on environmental radiation measurements; first woman ...
Using Agrobacterium-mediated transformation, a technique that takes advantage of the natural process by which Agrobacterium ... Zhang X, Henriques R, Lin SS, Niu QW, Chua NH (2006). "Agrobacterium-mediated transformation of Arabidopsis thaliana using the ... Genetic transformation of A. thaliana is routine, utilizing Agrobacterium tumefaciens to transfer DNA into the plant genome. ... The current protocol, termed "floral dip", involves simply dipping flowers into a solution containing Agrobacterium carrying a ...
... is a part of the genetic equipment that Agrobacterium tumefaciens and Agrobacterium rhizogenes use to transduce their genetic ... Agrobacterium is called the natural genetic engineer. Size of the plasmid: ~250 kbp. Contains one or more T-DNA region. ... The Ti plasmid is lost when Agrobacterium is grown above 28 °C. Such cured bacteria do not induce crown galls, i.e. they become ... Mary-Dell Chilton Jeff Schell Marc Van Montagu Schell J, Van Montagu M., The Ti-plasmid of Agrobacterium tumefaciens, a natural ...
"Genetic transformation of HeLa cells by Agrobacterium". Proc. Natl. Acad. Sci. U.S.A. 98 (4): 1871-6. doi:10.1073/pnas. ...
Her research topics included the Agrobacterium tumefaciens. Hohn is a member of the Austrian Academy of Sciences (Math and ... particularly known for her research into the Agrobacterium tumefaciens. Hohn was born Barbara Freiinger, in Klagenfurt, Austria ...
nov.; Agrobacterium meteori sp. nov.; Agrobacterium ferrugineum sp. nov., nom. Rev.; Agrobacterium gelatinovorum sp. nov., nom ... Agrobacterium ferrugineum was reclassified to Hoeflea marina. LSPN bacterio.net Straininfo of Hoeflea marina Peix, A. (2005). " ... Rev.; and Agrobacterium stellulatum sp. nov., nom. Rev". International Journal of Systematic Bacteriology. 42 (1): 133-43. doi: ... Ruger, H. -J.; Hofle, M. G. (1992). "Marine Star-Shaped-Aggregate-Forming Bacteria: Agrobacterium atlanticum sp. ...
Based on molecular data it was shown that the genus Agrobacterium is nested in Rhizobium and the Agrobacterium species ... Given the plant pathogenic nature of Agrobacterium species, it was proposed to maintain the genus Agrobacterium and the latter ... Farrand, S.; Van Berkum, P.; Oger, P. (2003). "Agrobacterium is a definable genus of the family Rhizobiaceae". International ... and the inclusion of all species of Agrobacterium Conn 1942 and Allorhizobium undicola de Lajudie et al. 1998 as new ...
Agrobacterium-like conjugation is also primarily used for dicots, but monocot recipients are not uncommon. Sexual conjugation ... Pan SQ, Jin S, Boulton MI, Hawes M, Gordon MP, Nester EW (July 1995). "An Agrobacterium virulence factor encoded by a Ti ... For example, the tumor-inducing (Ti) plasmid of Agrobacterium and the root-tumor inducing (Ri) plasmid of A. rhizogenes contain ... Agrobacterium-like conjugation complements other standard vehicles such as tobacco mosaic virus (TMV). While TMV is capable of ...
For instance, the plant pathogen Agrobacterium is a closer relative of Rhizobium than the Bradyrhizobium that nodulate soybean ... "Bacteria confused with rhizobia, including Agrobacterium taxonomy". Retrieved 2013-12-02. "Taxonomy of legume nodule bacteria ( ...
Agrobacterium tumefaciens Pierce's disease Xylella fastidiosa Bacterial inflorescence rot [1] Pseudomonas syringae ...
Agrobacterium'i liigid on taimedega seotud Rhizobia sugulased. Mitu Agrobactericumi liiki põhjustab taimehaigusi, nagu ... Agrobacterium-vahendatud taimede ümberkujundamine on väga keeruline protsess, mis hõlmab nii bakteri kui ka peremeestaime ... "Agrobacterium-Mediated Plant Transformation: the Biology behind the "Gene-Jockeying" Tool". 2003. American Society for ... Geenmuundatud organismide tehnoloogia aluseks on mehhanism, mida Agrobacterium kasutab oma T-DNA sisaldamiseks taimede ...
From Galacturonic acid by the organism agrobacterium tumefaciens. Alpha-ketoglutarate can be used to produce: Creatine-alpha ...
One of these was isolated from an Agrobacterium strain CP4 (CP4 EPSPS) that was resistant to glyphosate. The CP4 EPSPS gene was ... Agrobacterium tumefaciens-mediated transformation is another common technique. Agrobacteria are natural plant parasites, and ... Shrawat, A.; Lörz, H. (2006). "Agrobacterium-mediated transformation of cereals: a promising approach crossing barriers". Plant ... When Agrobacterium infects a plant, it transfers this T-DNA to a random site in the plant genome. When used in genetic ...
Agrobacterium, Caulobacter, Rickettsia, Wolbachia, etc. Betaproteobacteria: Bordetella, Ralstonia, Neisseria, Nitrosomonas, etc ...
Her research on Agrobacterium also showed that the genes responsible for causing disease could be removed from the bacterium ... Chilton was the first (1977) to demonstrate the presence of a fragment of Agrobacterium Ti plasmid DNA in the nuclear DNA of ... Chilton, M. D.; Tepfer, D. A.; Petit, A.; David, C.; Casse-Delbart, F.; Tempé, J. (1982). "Agrobacterium rhizogenes inserts T- ... She and her collaborators produced the first genetically modified plants using Agrobacterium carrying the disarmed Ti plasmid ( ...
Several Agrobacterium tumefaciens strains have been isolated for their ability to produce D-amino acids from D, L-substituted ... Regulation of hyu gene expression in Agrobacterium tumefaciens strains RU-AE01 and RU-OR. by Jiwaji, M. ...
Agrobacterium strains were attracted to several opines tested. The determinants for chemotaxis to these opines were localized ... Characterization of Theacc (Agrocinopine Catabolism) Locus on the Nopaline-Type Ti Plasmid From Agrobacterium Tumefaciens ... Characterization of Theacc (Agrocinopine Catabolism) Locus on the Nopaline-Type Ti Plasmid From Agrobacterium Tumefaciens ... Characterization of Theacc (Agrocinopine Catabolism) Locus on the Nopaline-Type Ti Plasmid From Agrobacterium Tumefaciens ...
Agrobacterium rhizogenes-mediated transformation: Root cultures as a source of alkaloids. Planta Medica. 2002;68(10):859-868. ... Agrobacterium rhizogenes-mediated transformation : Root cultures as a source of alkaloids. In: Planta Medica. 2002 ; Vol. 68, ... Agrobacterium rhizogenes-mediated transformation: Root cultures as a source of alkaloids. Nina Sevon, Kirsi-Marja Oksman- ... Agrobacterium rhizogenes-mediated transformation : Root cultures as a source of alkaloids. / Sevon, Nina; Oksman-Caldentey, ...
The plant pathogen Agrobacterium tumefaciens produces a unipolar polysaccharide (UPP) adhesin at single cell poles that contact ... Xu, J., Kim, J., Koestler, B. J., Choi, J-H., Waters, C. M., & Fuqua, C. (2013). Genetic analysis of agrobacterium tumefaciens ... Xu J, Kim J, Koestler BJ, Choi J-H, Waters CM, Fuqua C. Genetic analysis of agrobacterium tumefaciens unipolar polysaccharide ... The plant pathogen Agrobacterium tumefaciens produces a unipolar polysaccharide (UPP) adhesin at single cell poles that contact ...
Agrobacterium tumefaciens, Agrobacterium rhizogenes, and Agrobacterium vitis. Strains within Agrobacterium tumefaciens and ... Agrobacterium nepotum (Puławska et al. 2012) Mousavi et al. 2015. *Agrobacterium pusense (Panday et al. 2011) Mousavi et al. ... Agrobacterium rubi (Hildebrand 1940) Starr and Weiss 1943. *Agrobacterium skierniewicense (Puławska et al. 2012) Mousavi et al ... Agrobacterium tumefaciens is the most commonly studied species in this genus. Agrobacterium is well known for its ability to ...
Agrobacterium tumefacien C58 Genome Page - as sequenced by Cereon Genomics/University of Richmond Agrobacterium tumefacien C58 ... Agrobacterium tumefaciens (updated scientific name Rhizobium radiobacter, synonym Agrobacterium radiobacter) is the causal ... Agrobacterium tumefaciens overwinters in infested soils. Agrobacterium species live predominantly saprophytic lifestyles, so ... "Rhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium radiobacter)". UniProt Taxonomy. Archived from the original on ...
Brief Description of Agrobacterium tumefaciens C58:. A. tumefaciens C58 is the most heavily studied member of the genus ... Scientists know a lot now about virulent Agrobacterium strains do this and have worked out methods to use this natural DNA ... However, most people know Agrobacterium because some strains can do something that no other cellular pathogen does - inject a ... Agrobacterium. Members of this genus are commonly found in soils all over the world as a aerobic heterotroph that can use a ...
... Steve Wylie wylie at CENTRAL.MURDOCH.EDU.AU Tue Oct 10 21:20:46 EST 1995 *Previous message: Geneclean ... Does anyone else working with Agrobacterium tumefaciens have the problems that I do? Firstly, it is very inconsistant in growth ...
Now I want the blank cultures of Agrobacterium , strains, LBA 4404, EHA 105 and EHA 101 so that I can introduce my vector , ... Agrobacterium strains. Dr Engelbert Buxbaum engelbert_buxbaum at hotmail.com Thu Jun 8 07:13:43 EST 2006 *Previous message: How ... Can you please , tell me how to get these Agrobacterium strains. There are microbiological type culture collection, for example ...
MULTISPECIES: hydantoinase/oxoprolinase family protein [Agrobacterium]. NCBI Reference Sequence: WP_012649231.1. Identical ... MULTISPECIES: hydantoinase/oxoprolinase family protein [Agrobacterium] MULTISPECIES: hydantoinase/oxoprolinase family protein [ ...
The T-pilus of Agrobacterium tumefaciens.. Lai EM1, Kado CI.. Author information. 1. Davis Crown Gall Group, University of ... and protein-transport apparatus for the transport of cyclic T-pilin subunits to the Agrobacterium cell surface. T-pilin ... subunits are processed from full-length VirB2 pro-pilin into a cyclized peptide, a rapid reaction that is Agrobacterium ...
Gelvin SB (2012) Traversing the cell: Agrobacterium T-DNAs journey to the host genome. Front Plant Sci 3:52PubMedGoogle ... Gelvin SB (2008) Agrobacterium-mediated DNA transfer, and then some. Nat Biotechnol 26:998-1000PubMedGoogle Scholar ... The strategy relies on Agrobacterium as a vector to deliver DNA copies of one or more viral RNA/DNA replicons; the bacteria are ... Magori S, Citovsky V (2011) Epigenetic control of Agrobacterium T-DNA integration. Biochim Biophys Acta 1809:388-394PubMed ...
Agrobacterium is the only cellular organism on Earth that is naturally capable of transferring genetic material between the ... The Oncogenes of Agrobacterium Tumefaciens and Agrobacterium Rhizogenes Monica T. Britton, Matthew A. Escobar, Abhaya M. ... Agrobacterium is a comprehensive book on Agrobacterium research, including its history, application, basic biology discoveries ... Agrobacterium DNA Expression Pathogen Pathogene Transport biotechnology crown gall disease currentjks gene transfer genetic ...
Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium; ... Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58. ...
This volume reviews various facets of Agrobacterium biology, from modern aspects of taxonomy and bacterial ecology to ... Agrobacterium Biology. Book Subtitle. From Basic Science to Biotechnology. Editors. * Stanton B. Gelvin ... Agrobacterium Biology. From Basic Science to Biotechnology. Editors: Gelvin, Stanton B. (Ed.) ... Agrobacterium-mediated transformation is the most extensively utilized platform for generating transgenic plants, but modern ...
Agrobacterium agile, Agrobacterium albertimagni, Agrobacterium aurantiacum, Agrobacterium larrymoorei, Agrobacterium ... radiobacter, Agrobacterium rhizogenes, Agrobacterium rubi, Agrobacterium tumefaciens, Agrobacterium vitis, Agrobacterium sp. ... Agrobacterium tumefaciens is a Gram-negative, non-sporeforming, rod-shaped bacterium. Agrobacterium strains use different ... Agrobacterium tumefaciens str. C58 has an unusual chromosomal organization - it has a 2 Mb linear and a 2.8 Mb circular ...
This proteome is part of the Agrobacterium radiobacter pan proteome (fasta) Agrobacterium are Gram-negative, motile, soil- ... More recently Agrobacterium have been classified into 3 biovars based on physiological and biochemical phenotypes without ... "Genome sequences of three Agrobacterium biovars help elucidate the evolution of multichromosome genomes in bacteria.". Slater S ...
Agrobacterium tumefaciens is a Gram-negative soil bacterium that causes plant tumours by transferring a portion of DNA from a ...
Association of single-stranded transferred DNA from Agrobacterium tumefaciens with tobacco cells.. V M Yusibov, T R Steck, V ... Association of single-stranded transferred DNA from Agrobacterium tumefaciens with tobacco cells. ... Association of single-stranded transferred DNA from Agrobacterium tumefaciens with tobacco cells. ... Association of single-stranded transferred DNA from Agrobacterium tumefaciens with tobacco cells. ...
Nucleotide excision repair - Agrobacterium fabrum [ Pathway menu , Organism menu , Pathway entry , Download KGML , Show ...
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Certain plant-associated prokaryotes such as Corynebacteriurn fascians and Agrobacterium tumefaciens are known to produce ... Cytokinin production by Agrobacterium tumefaciens Public Deposited Citeable URL:. http://ir.library.oregonstate.edu/concern/ ...
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Glycoside Hydrolase Genes Are Required for Virulence of Agrobacterium tumefaciens on Bryophyllum daigremontiana and Tomato A. ... Expression of a Peptidoglycan Hydrolase from Lytic Bacteriophages Atu_ph02 and Atu_ph03 Triggers Lysis of Agrobacterium ...
Agrobacterium rhizogenes, Agrobacterium rubi, Agrobacterium tumefaciens, Agrobacterium vitis, Agrobacterium sp. ,h2> ... Agrobacterium agile, Agrobacterium albertimagni, Agrobacterium aurantiacum, Agrobacterium larrymoorei, Agrobacterium ... Interestingly, if Agrobacterium is grown near its maximum temperature of about 30,sup>o,/sup>C, then the plasmid is lost as ... Agrobacterium tumefaciens causes crown gall disease by first transferring part of its DNA into an opening in the plant. ...
  • Hairy roots, transformed with Agrobacterium rhizogenes, have been found to be suitable for the production of secondary metabolites because of their stable and high productivity in hormone-free culture conditions. (vtt.fi)
  • A number of plant species including many medicinal plants have been successfully transformed with Agrobacterium rhizogenes. (vtt.fi)
  • However there has been some opposition to renaming Agrobacterium , in this article I explain the research and taxonomy, and suggest a solution. (rhizobia.co.nz)
  • An optimized procedure, based on transformation efficiencies (measured as percentage of transformed explants and average gfp spots per transformed explant) and control of the Agrobacterium growth after co-culture, was established. (csic.es)
  • In roots as well as in shoots, Agrobacterium tumefaciens and E. coli triggered similar (in PR37Y15) or different (in DK315) changes in the high-performance liquid chromatography profiles of secondary metabolites (especially benzoxazinoids), distinct from those of Azospirillum brasilense UAP-154. (apsnet.org)
  • A chitinase gene, chiB, from Serratia marcescens was characterised and sequenced and, following removal of its promoter region, was linked to an Agrobacterium virB promoter. (dur.ac.uk)
  • The Agrobacterium method has been successfully practiced in dicots for many years, but only recently have efficient protocols been developed for grasses. (biomedsearch.com)