Agrobacterium tumefaciens: A species of gram-negative, aerobic bacteria isolated from soil and the stems, leafs, and roots of plants. Some biotypes are pathogenic and cause the formation of PLANT TUMORS in a wide variety of higher plants. The species is a major research tool in biotechnology.Rhizobium: A genus of gram-negative, aerobic, rod-shaped bacteria that activate PLANT ROOT NODULATION in leguminous plants. Members of this genus are nitrogen-fixing and common soil inhabitants.Agrobacterium: A genus of gram negative, aerobic, rod-shaped bacteria found in soil, plants, and marine mud.Plant Tumors: A localized proliferation of plant tissue forming a swelling or outgrowth, commonly with a characteristic shape and unlike any organ of the normal plant. Plant tumors or galls usually form in response to the action of a pathogen or a pest. (Holliday, P., A Dictionary of Plant Pathology, 1989, p330)Plant Tumor-Inducing Plasmids: Plasmids coding for proteins which induce PLANT TUMORS. The most notable example of a plant tumor inducing plasmid is the Ti plasmid found associated with AGROBACTERIUM TUMEFACIENS.Transformation, Genetic: Change brought about to an organisms genetic composition by unidirectional transfer (TRANSFECTION; TRANSDUCTION, GENETIC; CONJUGATION, GENETIC, etc.) and incorporation of foreign DNA into prokaryotic or eukaryotic cells by recombination of part or all of that DNA into the cell's genome.Bacterial Proteins: Proteins found in any species of bacterium.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Virulence Factors: Those components of an organism that determine its capacity to cause disease but are not required for its viability per se. Two classes have been characterized: TOXINS, BIOLOGICAL and surface adhesion molecules that effect the ability of the microorganism to invade and colonize a host. (From Davis et al., Microbiology, 4th ed. p486)Plasmids: Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.Plants: Multicellular, eukaryotic life forms of kingdom Plantae (sensu lato), comprising the VIRIDIPLANTAE; RHODOPHYTA; and GLAUCOPHYTA; all of which acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations.Genes, Bacterial: The functional hereditary units of BACTERIA.Kalanchoe: A plant genus of the family CRASSULACEAE. Members contain bryophyllins (also called bryotoxins) which are bufadienolides (BUFANOLIDES) that have insecticidal activity.Plants, Genetically Modified: PLANTS, or their progeny, whose GENOME has been altered by GENETIC ENGINEERING.Rhizobiaceae: A family of gram-negative bacteria which are saprophytes, symbionts, or plant pathogens.Tobacco: A plant genus of the family SOLANACEAE. Members contain NICOTINE and other biologically active chemicals; its dried leaves are used for SMOKING.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Zeatin: An aminopurine factor in plant extracts that induces cell division. (Grant & Hackh's Chemical Dict, 5th ed)Virulence: The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.Plants, Toxic: Plants or plant parts which are harmful to man or other animals.Gene Expression Regulation, Bacterial: Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.Conjugation, Genetic: A parasexual process in BACTERIA; ALGAE; FUNGI; and ciliate EUKARYOTA for achieving exchange of chromosome material during fusion of two cells. In bacteria, this is a uni-directional transfer of genetic material; in protozoa it is a bi-directional exchange. In algae and fungi, it is a form of sexual reproduction, with the union of male and female gametes.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Plant Diseases: Diseases of plants.4-Butyrolactone: One of the FURANS with a carbonyl thereby forming a cyclic lactone. It is an endogenous compound made from gamma-aminobutyrate and is the precursor of gamma-hydroxybutyrate. It is also used as a pharmacological agent and solvent.Genetic Complementation Test: A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.AcetophenonesAmino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Plant Roots: The usually underground portions of a plant that serve as support, store food, and through which water and mineral nutrients enter the plant. (From American Heritage Dictionary, 1982; Concise Dictionary of Biology, 1990)beta-Glucosidase: An exocellulase with specificity for a variety of beta-D-glycoside substrates. It catalyzes the hydrolysis of terminal non-reducing residues in beta-D-glucosides with release of GLUCOSE.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Chromosomes, Bacterial: Structures within the nucleus of bacterial cells consisting of or containing DNA, which carry genetic information essential to the cell.Sinorhizobium meliloti: A species of gram-negative, aerobic bacteria that causes formation of root nodules on some, but not all, types of sweet clover, MEDICAGO SATIVA, and fenugreek.Mutagenesis, Insertional: Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene or extragenic sequence. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA insertions into or adjacent to a cellular proto-oncogene can interrupt GENETIC TRANSLATION of the coding sequences or interfere with recognition of regulatory elements and cause unregulated expression of the proto-oncogene resulting in tumor formation.Arginine: An essential amino acid that is physiologically active in the L-form.Operon: In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.DNA Transposable Elements: Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.Datura stramonium: A plant species of the genus DATURA, family SOLANACEAE, that contains TROPANES and other SOLANACEOUS ALKALOIDS.Hygromycin B: Aminoglycoside produced by Streptomyces hygroscopicus. It is used as an anthelmintic against swine infections by large roundworms, nodular worms, and whipworms.GlucuronidasePlant Somatic Embryogenesis Techniques: The process of embryo initiation in culture from vegetative, non-gametic, sporophytic, or somatic plant cells.Cytokinins: Plant hormones that promote the separation of daughter cells after mitotic division of a parent cell. Frequently they are purine derivatives.Plant Leaves: Expanded structures, usually green, of vascular plants, characteristically consisting of a bladelike expansion attached to a stem, and functioning as the principal organ of photosynthesis and transpiration. (American Heritage Dictionary, 2d ed)Sequence Homology, Amino Acid: The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.beta-Glucans: Glucose polymers consisting of a backbone of beta(1->3)-linked beta-D-glucopyranosyl units with beta(1->6) linked side chains of various lengths. They are a major component of the CELL WALL of organisms and of soluble DIETARY FIBER.Brucella suis: A species of gram-negative bacteria, primarily infecting SWINE, but it can also infect humans, DOGS, and HARES.DNA, Single-Stranded: A single chain of deoxyribonucleotides that occurs in some bacteria and viruses. It usually exists as a covalently closed circle.Fabaceae: The large family of plants characterized by pods. Some are edible and some cause LATHYRISM or FAVISM and other forms of poisoning. Other species yield useful materials like gums from ACACIA and various LECTINS like PHYTOHEMAGGLUTININS from PHASEOLUS. Many of them harbor NITROGEN FIXATION bacteria on their roots. Many but not all species of "beans" belong to this family.Arabidopsis: A plant genus of the family BRASSICACEAE that contains ARABIDOPSIS PROTEINS and MADS DOMAIN PROTEINS. The species A. thaliana is used for experiments in classical plant genetics as well as molecular genetic studies in plant physiology, biochemistry, and development.Phosphoric Triester Hydrolases: A class of enzymes that catalyze the hydrolysis of one of the three ester bonds in a phosphotriester-containing compound.Species Specificity: The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.Mannitol: A diuretic and renal diagnostic aid related to sorbitol. It has little significant energy value as it is largely eliminated from the body before any metabolism can take place. It can be used to treat oliguria associated with kidney failure or other manifestations of inadequate renal function and has been used for determination of glomerular filtration rate. Mannitol is also commonly used as a research tool in cell biological studies, usually to control osmolarity.Quorum Sensing: A phenomenon where microorganisms communicate and coordinate their behavior by the accumulation of signaling molecules. A reaction occurs when a substance accumulates to a sufficient concentration. This is most commonly seen in bacteria.Genetic Vectors: DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.Protocatechuate-3,4-Dioxygenase: An enzyme that catalyzes the oxidation of protocatechuate to 3-carboxy-cis-cis-muconate in the presence of molecular oxygen. It contains ferric ion. EC 1.13.11.3.Glucans: Polysaccharides composed of repeating glucose units. They can consist of branched or unbranched chains in any linkages.Alphaproteobacteria: A class in the phylum PROTEOBACTERIA comprised mostly of two major phenotypes: purple non-sulfur bacteria and aerobic bacteriochlorophyll-containing bacteria.Transformation, Bacterial: The heritable modification of the properties of a competent bacterium by naked DNA from another source. The uptake of naked DNA is a naturally occuring phenomenon in some bacteria. It is often used as a GENE TRANSFER TECHNIQUE.Sequence Homology, Nucleic Acid: The sequential correspondence of nucleotides in one nucleic acid molecule with those of another nucleic acid molecule. Sequence homology is an indication of the genetic relatedness of different organisms and gene function.Gene Expression Regulation, Plant: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in plants.DNA-Binding Proteins: Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.DNA, Plant: Deoxyribonucleic acid that makes up the genetic material of plants.Periplasmic Binding Proteins: Periplasmic proteins that scavenge or sense diverse nutrients. In the bacterial environment they usually couple to transporters or chemotaxis receptors on the inner bacterial membrane.Acyl-Butyrolactones: Cyclic esters of acylated BUTYRIC ACID containing four carbons in the ring.Restriction Mapping: Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.Lycopersicon esculentum: A plant species of the family SOLANACEAE, native of South America, widely cultivated for their edible, fleshy, usually red fruit.Gene Transfer Techniques: The introduction of functional (usually cloned) GENES into cells. A variety of techniques and naturally occurring processes are used for the gene transfer such as cell hybridization, LIPOSOMES or microcell-mediated gene transfer, ELECTROPORATION, chromosome-mediated gene transfer, TRANSFECTION, and GENETIC TRANSDUCTION. Gene transfer may result in genetically transformed cells and individual organisms.Linaria: A plant genus of the family Plantaginaceae. Members contain linarin (also called acaciin).Plant Proteins: Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which VEGETABLE PROTEINS is available.Sequence Alignment: The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.Nitrogen Fixation: The process in certain BACTERIA; FUNGI; and CYANOBACTERIA converting free atmospheric NITROGEN to biologically usable forms of nitrogen, such as AMMONIA; NITRATES; and amino compounds.Genetic Engineering: Directed modification of the gene complement of a living organism by such techniques as altering the DNA, substituting genetic material by means of a virus, transplanting whole nuclei, transplanting cell hybrids, etc.Laccaria: A genus of white-spored mushrooms in the family Tricholomataceae. They form symbiotic partnerships (MYCORRHIZAE) with trees.Plants, Medicinal: Plants whose roots, leaves, seeds, bark, or other constituent parts possess therapeutic, tonic, purgative, curative or other pharmacologic attributes, when administered to man or animals.Chromobacterium: A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria occurring in soil and water. Its organisms are generally nonpathogenic, but some species do cause infections of mammals, including humans.Oxazines: Six-membered heterocycles containing an oxygen and a nitrogen.RNA, Bacterial: Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.Ion Channels: Gated, ion-selective glycoproteins that traverse membranes. The stimulus for ION CHANNEL GATING can be due to a variety of stimuli such as LIGANDS, a TRANSMEMBRANE POTENTIAL DIFFERENCE, mechanical deformation or through INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS.Replicon: Any DNA sequence capable of independent replication or a molecule that possesses a REPLICATION ORIGIN and which is therefore potentially capable of being replicated in a suitable cell. (Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)Ammonia-Lyases: Enzymes that catalyze the formation of a carbon-carbon double bond by the elimination of AMMONIA. EC 4.3.1.Phylogeny: The relationships of groups of organisms as reflected by their genetic makeup.Promoter Regions, Genetic: DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.Mutagenesis: Process of generating a genetic MUTATION. It may occur spontaneously or be induced by MUTAGENS.Soil Microbiology: The presence of bacteria, viruses, and fungi in the soil. This term is not restricted to pathogenic organisms.Nucleic Acid Hybridization: Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)Rhizobium leguminosarum: A species of gram-negative, aerobic bacteria that is found in soil and which causes formation of root nodules on some, but not all, types of field pea, lentil, kidney bean, and clover.Genome, Bacterial: The genetic complement of a BACTERIA as represented in its DNA.Symbiosis: The relationship between two different species of organisms that are interdependent; each gains benefits from the other or a relationship between different species where both of the organisms in question benefit from the presence of the other.DNA Restriction Enzymes: Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.Daucus carota: A plant species of the family APIACEAE that is widely cultivated for the edible yellow-orange root. The plant has finely divided leaves and flat clusters of small white flowers.Gossypium: A plant genus of the family MALVACEAE. It is the source of COTTON FIBER; COTTONSEED OIL, which is used for cooking, and GOSSYPOL. The economically important cotton crop is a major user of agricultural PESTICIDES.DNA Primers: Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.Hydroxybenzoates: Benzoate derivatives substituted by one or more hydroxy groups in any position on the benzene ring.Indoleacetic Acids: Acetic acid derivatives of the heterocyclic compound indole. (Merck Index, 11th ed)Medicago sativa: A plant species of the family FABACEAE widely cultivated for ANIMAL FEED.beta-Galactosidase: A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.Recombinant Fusion Proteins: Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.Open Reading Frames: A sequence of successive nucleotide triplets that are read as CODONS specifying AMINO ACIDS and begin with an INITIATOR CODON and end with a stop codon (CODON, TERMINATOR).Zea mays: A plant species of the family POACEAE. It is a tall grass grown for its EDIBLE GRAIN, corn, used as food and animal FODDER.Solanum tuberosum: A plant species of the genus SOLANUM, family SOLANACEAE. The starchy roots are used as food. SOLANINE is found in green parts.Culture Media: Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.Adipates: Derivatives of adipic acid. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain a 1,6-carboxy terminated aliphatic structure.CinnamatesPili, Sex: Filamentous or elongated proteinaceous structures which extend from the cell surface in gram-negative bacteria that contain certain types of conjugative plasmid. These pili are the organs associated with genetic transfer and have essential roles in conjugation. Normally, only one or a few pili occur on a given donor cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed, p675) This preferred use of "pili" refers to the sexual appendage, to be distinguished from bacterial fimbriae (FIMBRIAE, BACTERIAL), also known as common pili, which are usually concerned with adhesion.Phenotype: The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.GlutaratesPolygalacturonase: A cell wall-degrading enzyme found in microorganisms and higher plants. It catalyzes the random hydrolysis of 1,4-alpha-D-galactosiduronic linkages in pectate and other galacturonans. EC 3.2.1.15.

Performance and long-term stability of the barley hordothionin gene in multiple transgenic apple lines. (1/102)

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Gene knockdown by ihpRNA-triggering in the ectomycorrhizal basidiomycete fungus Laccaria bicolor. (2/102)

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Efficient Agrobacterium-based transient expression system for the production of biopharmaceuticals in plants. (3/102)

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Agrobacterium-mediated transformation of kabocha squash (Cucurbita moschata Duch) induced by wounding with aluminum borate whiskers. (4/102)

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Transformed hairy roots of Discaria trinervis: a valuable tool for studying actinorhizal symbiosis in the context of intercellular infection. (5/102)

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Characterization and crop production efficiency of diazotrophic bacterial isolates from coastal saline soils. (6/102)

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A mobile signal transported over a long distance induces systemic transcriptional gene silencing in a grafted partner. (7/102)

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FVE, an Arabidopsis homologue of the retinoblastoma-associated protein that regulates flowering time and cold response, binds to chromatin as a large multiprotein complex. (8/102)

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*Transfer DNA

Agrobacterium-mediated T-DNA transfer is widely used as a tool in biotechnology. For more than two decades, Agrobacterium ... To interact with host plant proteins many Agrobacterium virulence proteins encoded by vir genes. Agrobacterium vir genes ... plasmid of some species of bacteria such as Agrobacterium tumefaciens and Agrobacterium rhizogenes. The T-DNA is transferred ... thus forming the crown gall tumors typically induced by Agrobacterium tumefaciens infection. Whereas Agrobacterium rhizogenes ...

*Rhizobium

"Rhizobium/Agrobacterium group". National Center for Biotechnology Information (NCBI) taxonomy database. Retrieved 2012-05-02. ...

*Rhizobium rhizogenes

Agrobacterium rhizogenes insert T-DNA into the genome of the host plant root cells. nature. 1982; 295:432-4 Cardarelli M, ... Rhizobium rhizogenes (formerly Agrobacterium rhizogenes) is a Gram-negative soil bacterium that produces hairy root disease in ... "Agrobacterium rhizogenes T-DNA genes capable of inducing hairy root phenotype". Molecular and General Genetics 209(3):475-480. ... Intrieri, M. C. & Buiatti, M. (July 2001). "The horizontal transfer of Agrobacterium rhizogenes genes and the evolution of the ...

*Agrobacterium

... rhizogenes, and Agrobacterium vitis. Strains within Agrobacterium tumefaciens and Agrobacterium rhizogenes are ... "Proposal for Rejection of Agrobacterium tumefaciens and Revised Descriptions for the Genus Agrobacterium and for Agrobacterium ... Agrobacterium tumefaciens is the most commonly studied species in this genus. Agrobacterium is well known for its ability to ... The Agrobacterium genus is quite heterogeneous. Recent taxonomic studies have reclassified all of the Agrobacterium species ...

*Agrobacterium albertimagni

... is a species of arsenite-oxidizing bacterium. Salmassi, Tina M.; Venkateswaren, Kasthuri; Satomi, ... Trimble WL, Phung le T, Meyer F, Gilbert JA, Silver S (2012). "Draft genome sequence of Agrobacterium albertimagni strain AOL15 ... Agrobacterium albertimagni at the Encyclopedia of Life LPSN. ... "Oxidation of Arsenite by Agrobacterium albertimagni , AOL15, sp ...

*Agrobacterium tumefaciens

Agrobacterium tumefacien C58 Genome Page - as sequenced by Cereon Genomics/University of Richmond Agrobacterium tumefacien C58 ... Agrobacterium tumefaciens (updated scientific name Rhizobium radiobacter, synonym Agrobacterium radiobacter) is the causal ... Agrobacterium tumefaciens overwinters in infested soils. Agrobacterium species live predominantly saprophytic lifestyles, so ... "Rhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium radiobacter)". UniProt Taxonomy. Archived from the original on ...

*Allorhizobium vitis

Ophel, K; Kerr, A (1990). "Agrobacterium vitis sp. nov. for strains of Agrobacterium biovar 3 from grapevines". Int J Syst Evol ... "Organism Overview: Agrobacterium vitis". National Center for Biotechnology Information. U.S. National Library of Medicine. 28 ... A 1999 study suggested that A. vitis is more closely related to Rhizobium galegae than to other Agrobacterium species, based on ... "Crown gall (Agrobacterium vitis)". Government of British Columbia. British Columbia Ministry of Agriculture. January 2009. ...

*Tryptophan-phenylpyruvate transaminase

Sukanya NK, Vaidyanathan CS (1964). "Aminotransferases of Agrobacterium tumefaciens. Transamination between tryptophan and ...

*International Committee on Systematics of Prokaryotes

These include the following: Aeromonadaceae, Vibrionaceae and related organisms; Genera Agrobacterium and Rhizobium; Bacillus ...

*Extracellular polymeric substance

myxogenes) cyclosophorans (Agrobacterium spp., Rhizobium spp. and Xanthomonas spp.) dextran (Leuconostoc mesenteroides, ... Agrobacterium radiobacter, Pseudomonas marginalis, Rhizobium spp. and Zooglea' spp.) galactosaminogalactan (Aspergillus spp.) ...

*List of varieties of genetically modified maize

Agrobacterium tumefaciens-mediated plant transformation. MON 88017 is an Insect resistant maize expressing the Cry3Bb1 protein ...

*Cysteinyl leukotriene receptor 2

Van Keer C, Kersters K, De Ley J (September 1976). "L-Sorbose metabolism in Agrobacterium tumefaciens". Antonie Van Leeuwenhoek ...

*Glycosynthase

One example is the Agrobacterium sp. β-glucosythase, which forms a β 1-4 glycoside with Glucose as the acceptor, but forms a β ... The first reported glycosynthase was a mutant of the Agrobacterium sp. β-glucosidase / galactosidase in which the nucleophile ...

*List of Women in Technology International Hall of Fame inductees

... "queen of Agrobacterium." Eileen Gail de Planque, Ph.D. (1944-2010), expert on environmental radiation measurements; first woman ...

*Arabidopsis thaliana

Using Agrobacterium-mediated transformation, a technique that takes advantage of the natural process by which Agrobacterium ... Zhang X, Henriques R, Lin SS, Niu QW, Chua NH (2006). "Agrobacterium-mediated transformation of Arabidopsis thaliana using the ... Genetic transformation of A. thaliana is routine, utilizing Agrobacterium tumefaciens to transfer DNA into the plant genome. ... The current protocol, termed "floral dip", involves simply dipping flowers into a solution containing Agrobacterium carrying a ...

*Ti plasmid

... is a part of the genetic equipment that Agrobacterium tumefaciens and Agrobacterium rhizogenes use to transduce their genetic ... Agrobacterium is called the natural genetic engineer. Size of the plasmid: ~250 kbp. Contains one or more T-DNA region. ... The Ti plasmid is lost when Agrobacterium is grown above 28 °C. Such cured bacteria do not induce crown galls, i.e. they become ... Mary-Dell Chilton Jeff Schell Marc Van Montagu Schell J, Van Montagu M., The Ti-plasmid of Agrobacterium tumefaciens, a natural ...

*Outline of cell biology

"Genetic transformation of HeLa cells by Agrobacterium". Proc. Natl. Acad. Sci. U.S.A. 98 (4): 1871-6. doi:10.1073/pnas. ...

*Barbara Hohn

Her research topics included the Agrobacterium tumefaciens. Hohn is a member of the Austrian Academy of Sciences (Math and ... particularly known for her research into the Agrobacterium tumefaciens. Hohn was born Barbara Freiinger, in Klagenfurt, Austria ...

*Hoeflea marina

nov.; Agrobacterium meteori sp. nov.; Agrobacterium ferrugineum sp. nov., nom. Rev.; Agrobacterium gelatinovorum sp. nov., nom ... Agrobacterium ferrugineum was reclassified to Hoeflea marina. LSPN bacterio.net Straininfo of Hoeflea marina Peix, A. (2005). " ... Rev.; and Agrobacterium stellulatum sp. nov., nom. Rev". International Journal of Systematic Bacteriology. 42 (1): 133-43. doi: ... Ruger, H. -J.; Hofle, M. G. (1992). "Marine Star-Shaped-Aggregate-Forming Bacteria: Agrobacterium atlanticum sp. ...

*Bacterial taxonomy

Based on molecular data it was shown that the genus Agrobacterium is nested in Rhizobium and the Agrobacterium species ... Given the plant pathogenic nature of Agrobacterium species, it was proposed to maintain the genus Agrobacterium and the latter ... Farrand, S.; Van Berkum, P.; Oger, P. (2003). "Agrobacterium is a definable genus of the family Rhizobiaceae". International ... and the inclusion of all species of Agrobacterium Conn 1942 and Allorhizobium undicola de Lajudie et al. 1998 as new ...

*Bacterial conjugation

Agrobacterium-like conjugation is also primarily used for dicots, but monocot recipients are not uncommon. Sexual conjugation ... Pan SQ, Jin S, Boulton MI, Hawes M, Gordon MP, Nester EW (July 1995). "An Agrobacterium virulence factor encoded by a Ti ... For example, the tumor-inducing (Ti) plasmid of Agrobacterium and the root-tumor inducing (Ri) plasmid of A. rhizogenes contain ... Agrobacterium-like conjugation complements other standard vehicles such as tobacco mosaic virus (TMV). While TMV is capable of ...

*Rhizobia

For instance, the plant pathogen Agrobacterium is a closer relative of Rhizobium than the Bradyrhizobium that nodulate soybean ... "Bacteria confused with rhizobia, including Agrobacterium taxonomy". Retrieved 2013-12-02. "Taxonomy of legume nodule bacteria ( ...

*Alpha-Ketoglutaric acid

From Galacturonic acid by the organism agrobacterium tumefaciens. Alpha-ketoglutarate can be used to produce: Creatine-alpha ...

*Genetically modified crops

One of these was isolated from an Agrobacterium strain CP4 (CP4 EPSPS) that was resistant to glyphosate. The CP4 EPSPS gene was ... Agrobacterium tumefaciens-mediated transformation is another common technique. Agrobacteria are natural plant parasites, and ... Shrawat, A.; Lörz, H. (2006). "Agrobacterium-mediated transformation of cereals: a promising approach crossing barriers". Plant ... When Agrobacterium infects a plant, it transfers this T-DNA to a random site in the plant genome. When used in genetic ...

*Proteobacteria

Agrobacterium, Caulobacter, Rickettsia, Wolbachia, etc. Betaproteobacteria: Bordetella, Ralstonia, Neisseria, Nitrosomonas, etc ...
2017). Agrobacterium rhizogenes-mediated transformation of Peganum multisectum (Maxim) Bobrov and harmine production in hairy roots, Journal of Medicinal plants and By-product, 6(2), pp. 91-96 ...
Poplar is a model system for the regeneration and genetic transformation of woody plants. To shorten the time required for studies of transgenic poplar, efforts have been made to optimize transformation methods that use Agrobacterium tumefaciens. In this study, an Agrobacterium infective suspension was treated at 4 °C for at least 10 h before infecting explants. By transforming the Populus hybrid clone
Background Transient gene expression via Agrobacterium-mediated DNA transfer offers a simple and fast method to analyze transgene functions. Although Arabidopsis is the most-studied model plant with powerful genetic and genomic resources, achieving highly efficient and consistent transient expression for gene function analysis in Arabidopsis remains challenging. Results We developed a highly efficient and robust Agrobacterium-mediated transient expression system, named AGROBEST (Agrobacterium-mediated enhanced seedling transformation), which achieves versatile analysis of diverse gene functions in intact Arabidopsis seedlings. Using β-glucuronidase (GUS) as a reporter for Agrobacterium-mediated transformation assay, we show that the use of a specific disarmed Agrobacterium strain with vir gene pre-induction resulted in homogenous GUS staining in cotyledons of young Arabidopsis seedlings. Optimization with AB salts in plant culture medium buffered with acidic pH 5.5 during Agrobacterium infection
See on Scoop.it - Virology and Bioinformatics from Virology.caThe flagellotropic phage 7-7-1 infects motile cells of Agrobacterium sp H13-3 by attaching to and traveling along the rotating flagellar filament to the secondary receptor at the base, where it injects its DNA into the host cell. This is an interesting paper, because it describes a…
Sorghum is the fifth most widely planted cereal crop in the world and is commonly cultivated in arid and semi-arid regions such as Africa. Despite its importance as a food source, sorghum genetic improvement through transgenic approaches has been limited because of an inefficient transformation system. Here we report a ternary vector (also known as co-habitating vector) system using a recently described pVIR accessory plasmid that facilitates efficient Agrobacterium-mediated transformation of sorghum. We report regeneration frequencies ranging from 6-29% in Tx430 using different selectable markers and single copy, backbone free "quality events" ranging from 45-66% of the total events produced ...
We have established an efficient Agrobacterium-mediated transformation procedure for Arabidopsis thaliana genotype C24 using the chimeric bialaphos resistance gene (bar) coding for phosphinothricin ac
Transgenic tomato plants of south Indian cultivar Arka Vikas were developed using Agrobacterium strain EHA 105, harbouring Bt Cry2A gene with a construct containing 35S CaMV promoter, OCS terminator and nptII selectable marker, through Agrobacterium-mediated transformation. This study was conducted to improve the regeneration and transformation protocol for south Indian cultivar Arka Vikas. Hypocotyl was used as explant source for transformation due to high regeneration efficiency, molecular analysis through PCR for putative transformants in T0 generation and qualitative ELISA method was performed for Bt protein expression followed by insect bioassays. Insect bioassay studies was conducted using neonate larva of helicoverpa armigera to screen the plants and the plants expressing good resistance with molecular and phenotypic characters were carried further for successive generations. The experimental results concluded that Bt gene was deployed in tomato cultivar successfully and had developed resistance
Agrobacterium-mediated gene transfer to pre-organized meristematic tissue combined with axillary regeneration was standardized for transformation and regeneration of chickpea, which otherwise was difficult to achieve from other explants. Different Agrobacterium strains harbouring binary vectors pCGP1258, containing the GUS as a reporter and bar [gene for resistance to phosphinothricin (PPT)-the active ingredient of the herbicide Basta] as the selectable marker, were used for the transformation experiments. After co-cultivation, the shoot apex explants were transferred onto a PPT-free regeneration medium and their tops (2 mm) were thoroughly wetted with PPT solution (2 mg/mL). The multiple axillary shoots developing from the shoot apices were excised and placed onto a medium containing 10 mg/L PPT. The surviving shoots were subcultured every 2nd wk onto fresh medium containing 20 mg/L PPT. After each subculture, the number of surviving shoots decreased until it stabilized. Some of the chimeric ...
OpenPlant: sharing tools for a sustainable future. OpenPlant is a joint initiative in plant synthetic biology between the University of Cambridge, John Innes Centre and the Earlham Institute, funded by the BBSRC and EPSRC as part of the UK Synthetic Biology for Growth programme, directed in Cambridge by Prof. Jim Haseloff and in Norwich by Prof. Anne Osbourn.
OpenPlant: sharing tools for a sustainable future. OpenPlant is a joint initiative in plant synthetic biology between the University of Cambridge, John Innes Centre and the Earlham Institute, funded by the BBSRC and EPSRC as part of the UK Synthetic Biology for Growth programme, directed in Cambridge by Prof. Jim Haseloff and in Norwich by Prof. Anne Osbourn.
Standardized DNA assembly strategies facilitate the generation of multigene constructs from collections of building blocks in plant synthetic biology. A common syntax for hierarchical DNA assembly following the Golden Gate principle employing Type IIs restriction endonucleases was recently developed, and underlies the Modular Cloning and GoldenBraid systems. In these systems, transcriptional units and/or multigene constructs are assembled from libraries of standardized building blocks, also referred to as phytobricks, in several hierarchical levels and by iterative Golden Gate reactions. This combinatorial assembly strategy meets the increasingly complex demands in biotechnology and bioengineering, and also represents a cost-efficient and versatile alternative to previous molecular cloning techniques. For Modular Cloning, a collection of commonly used Plant Parts was previously released together with the Modular Cloning toolkit itself, which largely facilitated the adoption of this cloning ...
By Kan Wang. Rapid adjustments and demanding growth were made within the Agrobacterium box, similar to genetically reworking vegetation for either easy learn reasons and agricultural improvement. In Agrobacterium Protocols, 3rd version, Volumes 1 and 2, a crew of top specialists and veteran researchers describe intimately concepts for providing DNA to plant cells and completely changing their genomes. This variation emphasizes agricultural vegetation and plant species with financial values, with up to date protocols on 32 plant species and protocols concerning 19 new species. including the 1st and 2nd variants, those volumes provide Agrobacterium-mediated genetic transformation protocols for a complete of seventy six plant species. For a couple of very important crops similar to rice, barley, wheat and citrus, a number of protocols utilizing assorted beginning plant fabrics for transformation are included.. Volume 1 information up-to-date suggestions to be had for 18 plant species drawn from ...
Agrobacterium-mediated transformation: Agrobacterium-mediated transformation has been used to create commercial cultivars for over 10 years and is known to create insertion-site mutations (Table 2, Section 1.1). However, there has been only one large-scale study of the mutations created at insertion events2 containing single T-DNA3inserts (the type of event preferred for commercial purposes; Forsbach et al. 2003). In this study of 112 single-copy T-DNA insertion events in A. thaliana, the researchers found that exact T-DNA integration almost never occurred (Forsbach et al. 2003). Most of the T-DNA insertions resulted in small (1-100 base pair) deletions of plant genomic sequences at the insertion-site. However, for a significant number (24/112) there was evidence for large-scale rearrangement of plant genomic DNA at the insertion-site. Two of these insertion events contained chromosomal translocations. The rest had rearrangements which were not fully characterised. It is known, however, that ...
Profiles of Sina (upper line) and Sinb (lower line) gene expression in developing spikes of the non-transgenic plants of cv. Wanad from 8th to 32nd day after po
The following protocol is designed for NEB 10-beta Competent E. coli (NEB #C3019 ) which are included in the NEB PCR Cloning Kit (NEB #E1202 ) only
There is a broad range of methods that are employed in the process of producing GMOs. This often involves insertion of a gene of interest into living organisms depending on the species that you are working on. In plants mainly, two most common biotechnology-based techniques include; Agrobacterium-mediated transformation and bombardment of particles. According to the regulations given by FSANZ, it is a requirement that clear description of the method employed in genetically engineering plants is given.. Case study- Roundup Ready soy. This was produced using the particle bombardment method. This process of biotechnologically engineering soybeans involved; bombardment of the plant cells with microscopic particles of gold coated with DNA that contains the gene of interest. The gene of interest is the EPSPS gene that is derived from Agrobacterium. The aim of this is to introduce the novel gene of interest through the cell wall so that it integrates into the genetic material of the soy plant. The new ...
USING YEAST AS MODEL, A SCREENING WAS PERFORMED AND MON2 WAS IDENTIFIED AS A NOVEL HOST FACTOR THAT WAS INVOLVED IN AGROBACTERIUM-MEDIATED TRANSFORMATION (AMT) OF YEAST. FURTHER STUDIES HAVE REVEALED THAT MON2 MEDIATES TRANSIENT AMT NEGATIVELY. WHILE AT THE SAME TIME PLAY A POSITIVE ROLE IN MEDIATING STABLE AMT ...
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Za vse karantenske kodljive organizme bo tudi v prihodnje potrebno v Sloveniji zagotoviti temeljit nadzor, za karantenske kodljive organizme s seznama A1, ki so bili v Sloveniji odkriti v zadnjem asu, pa se bo treba opredeliti tudi do njihovega prihodnjega statusa. Tudi na splo no bo potrebno status karantenskih kodljivih organizmov na slovenskih karantenskih listah ponovno preveriti in jih uskladiti z Aneksom I Smernice 77/93/EC.. Portoro , 4. marca 1999. Poro ilo pripravila predsedujo a sekcije Jo i Jerman Cvelbar. Sekcija »Varstvo vinske trte«. Sekcija je obravnavala aktualno problematiko in novej a spoznanja na podro ju varstva vinske trte, diagnostike nekaterih problemati nih bolezni, zlasti raka vinske trte. S stali a prispevka k znanosti je potrebno posebej izpostaviti referata S. Vrabla o okolju prijazni metodi zbeganja pri zatiranju grozdnih suka ev ter M. abec o raku vinske trte (Agrobacterium vitis). Portoro , 4. marca 1999. Poro ilo pripravil predsedujo i sekcije dr. Stojan ...
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Synthetic biology is producing a paradigm shift in biotechnology based on the introduction of engineering principles in the design of new organisms by genetic modification (Check, 2005; Haseloff and Ajioka, 2009). Whereas synthetic biology has rapidly permeated microbial biotechnology, the engineering of multicelled organisms following synthetic biology principles is now emerging and is mainly driven by the so-called top-down approaches, where newly engineered genetic circuits are embedded into naturally existing organisms used as a "chassis." The plant chassis offers an extraordinarily fertile ground for synthetic biology-like engineering. However, technology still faces the huge challenge of performing engineering-driven genetic designs. One of the main technological challenges of plant synthetic biology requires the construction and transfer of multigene structures to the plant genome. This is putting pressure on developing DNA assembly and transformation technologies adapted to plants. One ...
Recent studies on the root proteome of Medicago truncatula (Gaertn.) showed an induction of pathogenesis-related (PR) proteins of the class 10 after infection with the oomycete pathogen Aphanomyces euteiches (Drechs.). To get insights into the function of these proteins during the parasitic root-microbe association, a gene knockdown approach using RNAi was carried out. Agrobacterium rhizogenes-mediated transformation of M. truncatula roots led to a knockdown of the Medicago PR10-1 gene in transgenic in vitro root cultures. Proteomic analyses of the MtPr10-1i root cultures showed that MtPr10-1 was efficiently knocked down in two MtPr10-1i lines. Moreover, five additional PR-10-type proteins annotated as abscisic acid responsive proteins (ABR17s) revealed also an almost complete silencing in these two lines. Inoculation of the root cultures with the oomycete root pathogen A. euteiches resulted in a clearly reduced colonization and thus in a suppressed infection development in MtPr10-1i roots as ...
Synthetic biology is an approach to engineering science. The stabilization of element, from screw threads to printed circuit boards, drives both the speed of innovation and the economy of production in mechanical and electronic engineering. This conceptual model is the basis of synthetic biology, where the principles of engineering are applied to biological systems. Synthetic biology is not what we are making; Developing tools and methods for the precise modification of plant genomes and establishing a standard structure for collection of plant genes.. ...
Soybean [Glycine max (L.) Merr] is one of the most important oil crops. Genetic transformation techniques can provide new tools for soybean improvement. The production of transgenic soybean has been limited. This research integrated a modified soybean regeneration system into a transformation protocol to optimize the production of transgenic soybean. Soybean hypocotyls and cotyledonary nodes were cultured on Gamborg B5 medium containing thidiazuron (TDZ). TDZ at 0.16 muM was found superior to other concentrations for inducing multiple shoot formation. Explants isolated from seedlings germinated on medium containing 6-benzyl-aminopurine (BAP) greater than 7 muM produced more multiple shoots than when on lower concentrations of BAP. Elongation of shoots was achieved on Gamborg B5 medium containing 0.36 muM BAP for hypocotyl-derived, or containing 0.58 mg/l gibberellin acid (GA3) and 0.67 mg/l indole-3-butyric acid (IBA) for cotyledonary node-derived multiple shoots. Plant recovery was achieved on medium
Mungbean is an important pulse crop extensively cultivated in Southeast Asia for supply of easily digestible protein. Salinity severely limits the growth and productivity of mungbean, and weeding poses nutritional and disease constraints to mungbean cultivation. To pyramid both salt tolerance and protection against herbicide in mungbean, the AtNHX1 encoding tonoplast Na+/H+ antiporter from Arabidopsis, and bar gene associated with herbicide resistance were co-expressed through Agrobacterium-mediated transformation. Stress inducible expression of AtNHX1 significantly improved tolerance under salt stress to ionic, osmotic and oxidative stresses in transgenic mungbean plants compared to the wild type (WT) plants, whereas constitutive expression of bar provided resistance to herbicide. Compared to WT, transgenic mungbean plants grew better with higher plant height, foliage, dry mass and seed yield under high salt stress (200 mM NaCl) in the greenhouse. The improved performance of transgenic plants under
The study was carried out to evaluate the amenability of tropical inbred and hybrid maize lines, using Agrobacterium mediated transformation technique. Agrobacteriumtumefaciens strains EHA101 harbouri
In an effort to engineer plants to be resistant to glyphosate, scientists starting looking for glyphosate resistant EPSP in the 1980s. Several methods were employed including selection, directed evolution, site-directed mutagenesis, and microbial screens. It was difficult to obtain a suitable enzyme because typically the resistant enzymes had an undesirable decrease in catalytic activity. Eventually, naturally occurring glyphosate-tolerant microbes were identified including Agrobacterium sp. Strain CP4, Achromobacter sp. Strain LBAA, and Pseudomonas sp. Strain PG2982. The enzymes isolate from these maintained good catalyctic ativity while being resistant to glyphosate (Funke). Agrobacterium sp. Strain CP4 was obtained from a glyphosate rich waste area at a glyphosate production facility. There is substantial sequence variation between these resistant enzymes and those of plants or E. coli. Other versions resistant versions of EPSP have been identified in Streptococcus pneumonia and ...
Here, we have demonstrated that the suppression of NHEJ-related gene expression causes decreased Agrobacterium-mediated stable transformation of the rice genome, especially in KD-OsLig4 (Figs 2, 3). Lig4 proteins interact with XRCC4 and catalyze the ligation step of DSBs in the NHEJ pathway (Mladenov & Iliakis, 2011; Symington & Gautier, 2011). The most generally accepted mechanisms of T-DNA integration into the plant genome are the strand-invasion model and the DSB repair model depending on the NHEJ pathway. The results presented here suggest that NHEJ is the major pathway of T-DNA integration into the rice genome, at least when rice scutellum-derived calli are used for transformation, and that the OsLig4 protein may play an important role in this process.. The decreased stable transformation observed in KD-OsKu70 and KD-OsKu80 plants and OsKu70+/− plants is in accordance with a previous report (Li et al., 2005), in which a decreased frequency of T-DNA integration was observed in atku80 ...
Hello researchers, , ,I am study one gene promoter and fused it to GUS in pCAMBIA1391Z, in which ,CaMV35S drives the selectable marker Hyg gene. Among the 10 independent T3 ,lines, which were confirmed by PCR, some show very strong GUS staining, ,some no, and some weak staining. , ,I knew from this paper that vectors containing 35S promoter driving ,selectable marker gene are not good for promoter study. ,The 35S promoter used in a selectable marker gene of a plant transformation ,vector affects the expression of the transgene. Planta. Volume 221, Number ,4 / June, 2005 , ,But I found that quite many published papers used vectors (like ,pCAMBIA1391, pCAMBIA1301, etc) containing 35S promoter driving selectable ,marker. , ,As the staining patterns of my promoter fusion lines are not consistent at ,al, I want to use another vector pBI121 or pCGN1547. Does anyone know where ,to get pCGN1547? ,How do you deal with those lines from 35S containing vector? CAMBIA ,suggests doing co-transformation ...
With current advances in sequencing technologies (link to sequencing section), however, blight-resistant genes from Chinese and Japanese chestnuts may soon be identified, cloned, and used in this process.. After you have your gene complex is created, the next step is to figure out a way to get it into your species of interest. There are a few ways to do this, but the easiest and current method used for chestnut is Agrobacterium-mediated transformation. After the transformation is completed, the next stage is to get the tree out into the field. There are several steps involved in this, and the resulting treelets need to be handled very carefully until they are ready to be put outside. Transformed embryonic tissue need to first be multiplied and start turning into shoots. The resulting shoots are then rooted in tissue culture boxes, where only 10-60% of them may actually develop roots. After the rooting cycle, the treelets need to be acclimatized and readied for the outdoors ...
is provided, focused on the role played by the different components of the virulence system. The general assessments for the establishment of efficient transformation protocols are discussed with an emphasis in the application of this methodology to monocotyledonous plants. Based on our own experience, we present the establishment of sugarcane transformation by A. tumefaciens as a model of application of this methodology to an important culture plant species, previously considered recalcitrant and inaccessible for this type of genetic manipulation ...
Triplex End-Point PCR kit. "The major symptoms of infection by Agrobacterium vitis are galls found on the lower trunk, near the soil line, but galls can also develop slightly below the soil surface, or extend up to 1-m height. Infected plants produce inferior shoots, and portions of the vine above the gall may die. A. vitis mainly enters the plant through wounds caused by freezing, so climatic conditions favouring freeze injury also favour infection by A. vitis. Contaminated planting material can, however, also be a source of inoculum.". Source: EPPO Bulletin 32, 367-369. ...
Agrobacterium vitis genes for 16S rRNA, tRNA-Ile, tRNA-Ala, 23S rRNA 150-b fragment, IVS, 23S rRNA 1.3-kb fragment A, partial and complete sequence, strain: NCPPB-1771, locus: ...
A enzima mio-inositol-1-fosfato sintase (MIPS1) (E.C.5.5.1.4) catalisa a conversão irreversível de D-Glicose-6-P para 1-L-mio-inositol-1-P. O mio-inositol desempenha papel de destaque no metabolismo vegetal, fornecendo inositol e inositídeos em processos metabólicos essenciais à formação do vegetal. Dentre as várias vias nas quais o mio-inositol está envolvido algumas são alvo do melhoramento vegetal, a exemplo a manipulação do conteúdo de ácido fítico e de oligossacarídeos em sementes e açúcares estruturais na parede celular. Até o presente momento, plantas MIPS silenciadas não foram caracterizadas em detalhes, principalmente em suas estruturas vegetativas. Com isso, o presente trabalho objetivou caracterizar tomateiros Moneymaker com diferentes níveis de silenciamento de MIPS1. Para a indução do silenciamento utilizou- se tranformação genética via Agrobacterium tumefaciens contendo plasmídeo pCambia com construção tipo intron hairpin de fragmentos do gene MIPS de ...
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Co-cultivation of production cells at the limit of the in vitro age is an assay we offer to test for latent retroviruses for our clients. Essentially, your production cells are plated along with susceptible cell lines that exhibit stronger phenotypic CPE when the virus enters them. Thus, viruses that are expressed as non-infectious particles or exhibit low level of expression in production cells are detected ...
Graduates of Bu-Ali Sina University - the names, photos, skill, job, location. Information on the Bu-Ali Sina University - contacts, students, faculty, finances.
一基本信息姓名:周虚出生年月:1965年8月15日生职称:吉林大学动物科学学院教授、博导联系方式:0431-87835142,Email:[email protected]招生学科:动物遗传育种与繁殖研究方向:动物生殖调控二研究特色1.牛、猪卵泡和早期胚胎发育调控的分子机制2.营养对母猪繁殖的影响及其内分泌和分子机制3.猪繁殖障碍检测与防治三学术任职教育部高校教学指导委员会委员中国奶业协会繁殖专业委员会副主任中国畜牧兽医学会动物繁殖分会常务理事、副秘书长吉林省畜牧兽医学会常务理事四学历和学位1981
Peanut, an important oilseed crop, is gaining priority for the development of drought tolerant genotypes in recent times, since the area under drought is constantly on the rise. To achieve this, one of the important strategies is to genetically engineer the ruling peanut varieties using transcription factor regulating the expression of several downstream, abiotic-stress responsive gene(s). In this study, eight independent transgenic peanut (cv. GG20) lines were developed using AtDREB1A gene, encoding for a transcription factor, through Agrobacterium-mediated genetic transformation. The transgene insertion was confirmed in (T0) using PCR and Dot-blot analysis, while copy-number(s) was ascertained using Southern-blot analysis. The inheritance of AtDREB1A gene in individual transgenic plants (T1 and T2) was confirmed using PCR. In homozygous transgenic plants (T2), under soil-moisture deficit stress, elevated level of AtDREB1A transgene expression was observed by RT-PCR assay. The transgenic plants at 45-d
Finger millet (Eleusine corocana subsp. coracana), an important cereal in East Africa and India, is a tetraploid species with unknown genomic components. A recent cytogenetic study confirmed the direct origin of this millet from the tetraploid E. coracana subsp. africana but questioned Eleusine indica as a genomic donor. Chloroplast (ct) DNA sequence analysis using restriction fragment pattern was used to examine the phylogenetic relationships between E. coracana subsp. coracana (domesticated finger millet), E. coracana subspecies africana (wild finger millet), and E. indica. Eleusine tristachya was included since it is the only other annual diploid species in the genus with a basic chromosome number of x = 9 like finger millet. Eight of the ten restriction endonucleases used had 16 to over 30 restriction sites per genome and were informative. E. coracana subsp. coracana and subsp. africana and E. indica were identical in all the restriction sites surveyed, while the ct genome of E, tristachya ...
RESISTENZGENE (GENETIK); PROMOTOR (MOLEKULARE GENETIK); TRANSGENE PFLANZEN + GENETISCHE TRANSFORMATION (PFLANZENGENETIK); NICOTIANA (BOTANIK); DIPLOMARBEITEN UND EXAMENSARBEITEN (DOKUMENTENTYP); RESISTANCE GENES (GENETICS); PROMOTER (MOLECULAR GENETICS); TRANSGENIC PLANTS + GENETIC TRANSFORMATION (PLANT GENETICS); NICOTIANA (BOTANY); EXAMINATION PAPERS + DEGREE PAPERS (DOCUMENT TYPES ...
kezhvaragu/finger millet - the centre of attraction First in the series of Dosais would be Kezhvaragu Dosai. Kezhvaragu in Tamil, is Finger Millet in English and locally Ragi in Karnataka, the southern Indian state which is the largest producer of Finger Millet in India. It is also called Keppai in Tamil. I give…
Nagel R., Elliott A., Masel A., Birch R.G. and Manners J.M. (1990) Electroporation of binary Ti plasmid vector into Agrobacterium tumefaciens and Agrobacterium rhizogenes. , 67 3: 325-328. doi:10.1016/0378-1097(90)90017-K ...
Manipulation of tropical maize in tissue culture is crucial to harnessing the benefits of biotechriology, especially those involving upgrading the genetic base of the maize to resist or tolerate biotic and abiotic constraints of production. The strategies involved are many, having transformed from those involving conventional breeding to the more appropriate molecular plant breeding methods. Transformation technology as a research or breeding tool to improve maize is routinely used in most industrial and some specialized public laboratories. However, transformation of many tropical maize inbred lines remains a challenging task, especially when using Agrobacterium tumefaciens as the transgene delivery method. Availability of very efficient Agrobacterium-mediated maize inbred line transfonriation protocol will improve future opportunities for maize genetic manipulation and functional genomics. Most molecular and gene function studies in maize requiring tissue culture have been done using cell ...
Background Agrobacterium tumefaciens has long been known to transform plant tissue in nature as part of its infection process. This natural mechanism has been utilised over the last few decades in laboratories world wide to genetically manipulate many species of plants. More recently this technology has been successfully applied to non-plant organisms in the laboratory, including fungi, where the plant wound hormone acetosyringone, an inducer of transformation, is supplied exogenously. In the natural environment it is possible that Agrobacterium and fungi may encounter each other at plant wound sites, where acetosyringone would be present, raising the possibility of natural gene transfer from bacterium to fungus. Methodology/Principal Findings We investigate this hypothesis through the development of experiments designed to replicate such a situation at a plant wound site. A. tumefaciens harbouring the plasmid pCAMDsRed was co-cultivated with the common plant pathogenic fungus Verticillium albo-atrum
Agrobacterium-mediated transformation and direct gene transfer using the gene gun (microparticle -bombardment) are the two most widely used methods for plant genetic modification. The Agrobacterium method has been successfully practiced in dicots for
Tef (Eragostis tef) and finger millet (Eleusine coracana) are both cereal crops that originated in East Africa. They belong to the Chloridoid sub-family of the grass family (Gramineae), and are hardy, resilient crops that exhibit good tolerance to abiotic stress, and produce small-sized grain with superior nutritional and storage characteristics. Both crops are of strategic importance to the food security of millions of African smallholder farmers; tef is the major staple food in Ethiopia while finger millet is an important traditional food crop in several Eastern, Central, and South African countries. These two crops, however, are among the least studied of the cereals in terms of crop improvement ...
Agrobacterium-mediated transformation is the most widely used technique for generating transgenic plants. However, many crops remain recalcitrant. We found that an Arabidopsis myb family transcription factor (MTF1) inhibited plant transformation susceptibility. Mutating MTF1 increased attachment of several Agrobacterium strains to roots and increased both stable and transient transformation in both susceptible and transformation-resistant Arabidopsis ecotypes. Cytokinins from Agrobacterium tumefaciens decreased the expression of MTF1 through activation of the cytokinin response regulator ARR3. Mutating AHK3 and AHK4, genes that encode cytokinin-responsive kinases, increased the expression of MTF1 and impaired plant transformation. Mutant mtf1 plants also had increased expression of AT14A, which encodes a putative transmembrane receptor for cell adhesion molecules. Plants overexpressing AT14A exhibited increased susceptibility to transformation, whereas at14a mutant plants exhibited decreased attachment
Artificial microRNA (amiRNA) technology utilizes microRNA (miRNA) biogenesis pathway to produce artificially selected small RNAs using miRNA gene backbone. It provides a feasible strategy for inducing loss of gene function, and has been applied in functional genomics study, improvement of crop quality and plant virus disease resistance. A big challenge in amiRNA applications is the unpredictability of silencing efficacy of the designed amiRNAs and not all constructed amiRNA candidates would be expressed effectively in plant cells. We and others found that high efficiency and specificity in RNA silencing can be achieved by designing amiRNAs with perfect or almost perfect sequence complementarity to their targets. In addition, we recently demonstrated that Agrobacterium-mediated transient expression system can be used to validate amiRNA constructs, which provides a simple, rapid and effective method to select highly expressible amiRNA candidates for stable genetic transformation. Here, we describe ...
In Article ,19940117170936.bloksber at thomashaw-at.css.msu.edu, bloksber at pilot.msu.edu (Leonard N. Bloksberg) says: , In Article ,1994Jan17.130735.1 at molbiol.ox.ac.uk, mknight at molbiol.ox.ac.uk says: , , Can anyone out there enlighten me as to what goes into the choice of which , , Agrobacterium strain to use for transformations? Im recommended by most people , , to use C58 and told that LBA4404 is a bad idea. Why is this? , , , , Many thanks! , , , , , , Marc Knight , , ==== ====== , , , . , LBA4404 is not really a bad idea, its just not the best. When people have , bothered to do side by side controled comparisons, Nopaline strains (eg, , C58) give higher rates of transformation with transgenes. Also, some of the , Octapine strains (eg the parent of LBA4404) still retain some of their onc , genes, and can yield strange and mostly sterile transgenics (not so for , LBA4404, as far as I know). LBA4404 contains the plasmid pAL4404 which is , a HindIII partial digest of the original ...
Hot pepper is one of the economically important crops in Asia. A large number of gene sequences, including expressed sequence tag (EST) and genomic sequences are publicly available. However, it is still a daunting task to determine gene function due to difficulties in genetic modification of a pepper plants. Here, we show the application of the virus-induced gene silencing (VIGS) repression for the study of 459 pepper ESTs selected as non-host pathogen-induced cell death responsive genes from pepper microarray experiments in Nicotiana benthamiana. Developmental abnormalities in N. benthamiana plants are observed in the 32 (7%) pepper ESTs-silenced plants. Aberrant morphological phenotypes largely comprised of three groups: stunted, abnormal leaf, and dead. In addition, by employing the combination of VIGS and Agrobacterium-mediated transient assays, we identified novel pepper ESTs that involved in Bax or INF1-mediated cell death responses. Silencing of seven pepper ESTs homologs suppressed Bax or INF1
Fluorescent nanodiamonds (FNDs) are promising nanoprobes, owing to their stable and magnetosensitive fluorescence. Therefore they can probe properties as magnetic resonances, pressure, temperature or strain. The unprecedented sensitivity of diamond defects can detect the faint magnetic resonance of a single electron or even a few nuclear spins. However, these sensitivities are only achieved if the diamond probe is close to the molecules that need to be detected. In order to utilize its full potential for biological applications, the diamond particle has to enter the cell. Some model systems, like HeLa cells, readily ingest particles. However, most cells do not show this behavior. In this article we show for the first time generally applicable methods, which are able to transport fluorescent nanodiamonds into cells with a thick cell wall. Yeast cells, in particular Saccharomyces cerevisiae, are a favored model organism to study intracellular processes including aging on a cellular level. In order ...
1 (Applied Biosystems, CA, USA) and was tested using the qPCR reaction conditions and the specific primers as indicated in point 2.4. The t35S pCAMBIA Sybricon plasmid was registered under "Safe Deposit" at the "Belgian Culture Collection for Micro-organisms" in the "Plasmid and DNA Library Collection" (BCCM/LMBP, Gent, Belgium; BCCM number: LMBP 8352). Authenticity was assessed by the BCCM/LMBP prior to acceptance. and certification (Barbau-Piednoir et al., 2010 and Broeders et al., 2012c). The assay was performed using 100 ng of 100% Bt rice DNA (Fig. 1). Degenerated random tagging (DRT) and Universal tagging Tariquidar mouse primers (UAP-N1 and N2) were provided by APAgene™ GOLD Genome Walking Kit (BIO S&T, Montréal, Canada). Recombinant Taq DNA Polymerase (10342; INVITROGEN, CA, USA) was used to synthesise DNA. The three gene-specific primers for t35S. pCAMBIA were designed as described above (Section 2.3). The t35S pCAMBIA a-R primer was used to perform the DNA ...
Finger millet health benefits include weight loss, diabetes control, stronger bones, better digestion, cancer prevention, etc. Learn the recipes to include finger miller in diet.
Title: Isolation of morphovariants through plant regeneration in ,i,Agrobacterium rhizogenes,/i, induced hairy root cultures of ,i,Plumbago rosea,/i, L. Authors: Satheeshkumar, K; Jose, Binoy; Soniya, E V; Seeni, S Abstract: ,smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="metricconverter", ,i style="",In vitro ,/i,raised shoots of ,i,Plumbago rosea,/i, L. were infected with A4 strain of ,i,Agrobacterium rhizogenes,/i, to initiate hairy root formation, which produced 3.0±0.33 hairy roots per incision on explants in 20 d incubation. Southern blot analysis confirmed the integration of T-DNA into the genome of the roots. The hairy roots were cultured on MS agar medium supplemented with 2.0 mg/L BAP to induce the formation of shoots (3.2±0.24) of 0.2-0.4 cm length in 7-8 wks. Isolated shoots were multiplied through sub culturing in the presence of 0.5 mg/L BAP and the resultant shoots were subjected to combined elongation (3.29±0.16 cm) and rooting (12.6±0.57) in a ...
A novel system for detection of gene transfer between A. tumefaciens and mammalian cells was established. Using this system, Agrobacterium was found to be able to deliver DNA located on its plasmid and chromosome into human cells. Agrobacterium is actively involved in this process; such a kind of DNA transfer might occur widely between bacteria and mammalian cells. The frequency of such a gene transfer was approximately 10-4-10-5 transformants per recipient. Our data showed that this DNA transfer is dependent upon Agrobacterium and independent of its virulence genes. The polymerization of actin played an important role in Agrobacterium internalization and subsequent gene transfer into mammalian cells. In addition, an Agrobacterium sensor protein ChvG was found to be important for the gene transfer into both plant and mammalian cells. The data suggested that ChvG was involved in the regulation of acid-inducible genes and might function as a global sensor protein that can directly or indirectly ...
We are looking at the functionality of select, drought-responsive genes identified in the interaction network by transferring them into rice using Agrobacterium-mediated transformation. The genetically engineered rice plants evaluated for their growth responses to drought stress under greenhouse simulations would help our understanding of rice plant biology under field conditions as well as improve its adaptability, which will contribute to the sustainable rice production worldwide.. ...
Kanamycin - Get up-to-date information on Kanamycin side effects, uses, dosage, overdose, pregnancy, alcohol and more. Learn more about Kanamycin
This protocol is a variant of the Hanahan protocol [1] using CCMB80 buffer for DH10B, TOP10 and MachI strains. It builds on Example 2 of the Bloom05 patent as well. This protocol has been tested on TOP10, MachI and BL21(DE3) cells. See Bacterial Transformation for a more general discussion of other techniques. The Jesse 464 patent describes using this buffer for DH5α cells. The Bloom04 patent describes the use of essentially the same protocol for the Invitrogen Mach 1 cells. This is the chemical transformation protocol used by Tom Knight and the Registry of Standard Biological Parts. ...
The transformed plates didnt look good. On the experiment plates 1-3, there were MANY colonies on the edges of the plates, but the middle of the plate had streakyness of something. On control plates 4-6, there were no colonies, but there was the same streakyness all across the plate. A few thing could have gone wrong such as the transformation protocol, the LB + Amp plates, or the HB cells. We will see how the colony PCR turns out for plates 1-3. We took 6 colonies from plates 1 and 2, and 1 colony from plate 3 ...
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La transformación genética es una técnica atractiva para el mejoramiento de los cítricos ya que evita los largos periodos juveniles y permite introducir nuevas características a un cultivar sin alterar los rasgos existentes. Un paso indispensable para aplicar esta tecnología de mejoramiento de los cítricos, es el desarrollo de sistemas eficientes de transformación genética que confieran ventajas...
The ExpiCHO transient expression system allows for the high titer production of a broad range of recombinant proteins. Due to the high titers observed for many proteins in the ExpiCHO system,
DOCTYPE html, ,html, ,head, ,title,HTML5 Experiment: A Rotating Solid Cube,/title, ,script type=text/javascript, window.onload = startDemo; function Point3D(x,y,z) { this.x = x; this.y = y; this.z = z; this.rotateX = function(angle) { var rad, cosa, sina, y, z rad = angle * Math.PI / 180 cosa = Math.cos(rad) sina = Math.sin(rad) y = this.y * cosa - this.z * sina z = this.y * sina + this.z * cosa return new Point3D(this.x, y, z) } this.rotateY = function(angle) { var rad, cosa, sina, x, z rad = angle * Math.PI / 180 cosa = Math.cos(rad) sina = Math.sin(rad) z = this.z * cosa - this.x * sina x = this.z * sina + this.x * cosa return new Point3D(x,this.y, z) } this.rotateZ = function(angle) { var rad, cosa, sina, x, y rad = angle * Math.PI / 180 cosa = Math.cos(rad) sina = Math.sin(rad) x = this.x * cosa - this.y * sina y = this.x * sina + this.y * cosa return new Point3D(x, y, this.z) } this.project = function(viewWidth, viewHeight, fov, viewDistance) { var factor, x, y factor = fov / ...
3 well silicone insert with two defined cell-free gaps, suitable for wound healing, migration assays, 2D invasion assays, and co-cultivation of cells Complet...
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Majoriteten av svenskarna borstar sina tänder på fel sätt. Totalt, fullbordade 2 023 individer frågeformuläret. Majoriteten (84 till 94 procent) i alla åldersgrupperna borstade sina tänder två gånger om dagen eller oftare.
Steviol glycosides in the present evaluation are mixtures of steviol glycosides that comprise not less than 95% of stevioside and/or rebaudioside A. Stevioside as a sweetener was evaluated by the SCF in 1984, 1989 and 1999. JECFA reviewed the safety of steviol glycosides in 2000, 2005, 2006, 2007, and 2009 and established an ADI for steviol glycosides (expressed as steviol equivalents) of 4 mg/kg bw/day. The Panel considers that the results of toxicology studies on either stevioside or rebaudioside A are applicable for the safety assessment of steviol glycosides as both rebaudioside A and stevioside are metabolised and excreted by similar pathways, with steviol being the common metabolite for both. Considering the available toxicity data (in vitro and in vivo animal studies and some human tolerance studies), the Panel concludes that steviol glycosides, complying with JECFA specifications, are not carcinogenic, genotoxic or associated with any reproductive/developmental toxicity. The Panel ...
Plant transformation provides a promising methodology of introducing new genes that encode desirable traits to a wide range of crop plants. Success in genetic transformation has been achieved in many of the important crop species, such as soybean, cotton, rice, corn. However, wheat, one of the major crops of the world, has been considered to be difficult to transform via either Agrobacterium or biolistic bombardment (Rakszegi et al., 2001). There have been limited studies on A. tumefaciens-mediated transformation of cereals, including wheat, because of the overall refractory character of host-pathogen interactions between Agrobacterium and the cereal plants (Gould et al., 1991; Hiei et al., 1994; Cheng et al., 1997). While the genetic transformation of rice using Agrobacterium has become routine, only a few successful studies of Agrobacterium- mediated transformation of wheat have been reported, and these involved a model spring wheat, Triticum aestivum cultivar Bobwhite (Cheng et al., 1997). ...
Coloured scanning electron micrograph (SEM) of Agrobacterium tumefaciens, Gram-negative, plant pathogen, rod bacterium. Agrobacterium tumefaciens is an alphaproteobacteria from the family Rhizobiaceae and is known to cause tumours in plants. It causes crown gall disease (formation of tumours disease) in over 140 species of dicot plants. A. tumefaciens is a serious pathogen of grape vines, stone fruits, nut trees, sugar beets, horse radish and rhubarb. Agrobacterium sp. can transfer DNA between itself and plants and thus has become an important tool for genetic manipulation (genetic engineering). Most Agrobacterium sp. have plasmids that they can be inject into plant cells at wound sites. This plasmid DNA is designed to integrate into the chromosomes of the infected plants cellular DNA. DNA transfer from Agrobacterium tumefaciens to eukaryotic plant cells is the only known example of interkingdom DNA transfer. Magnification: x3,000 when shortest axis - Stock Image C032/2375
Toxicological testing showed that the substances are not genotoxic, nor carcinogenic, or linked to any adverse effects on the reproductive human system or for the developing child. The Panel set an Acceptable Daily Intake (ADI)[1] of 4 mg per kg body weight per day for steviol glycosides, a level consistent with that already established by the Joint FAO/WHO Expert Committee on Food Additives (JECFA).. The Panel points out, however, that this ADI could be exceeded by both adults and children if these sweeteners are used at the maximum levels proposed by the applicants.. Steviol glycosides are intense sweeteners extracted from the leaves of the stevia plant (Stevia rebaudiana Bertoni). These substances, such as stevioside and rebaudioside, range in sweetness from 40 to 300 times sweeter than sucrose.. EFSA reviewed the safety of three dossiers supporting requests for authorisation. Food additives such as sweeteners must be explicitly authorised at European level before they can be used in ...
Although Agrobacterium tumefaciens has been successfully used to transfer genes to a wide range of plant species, it has received little attention for transformation of forage grasses. Therefore, the objective of the present study was to demonstrate Agrobacterium-mediated transformation of switchgrass (Panicum virgatum L.). The A. tumefaciens strain AGL 1 carrying the binary vector pDM805, coding for the phosphinothricin acetyltransferase (bar) and β-glucuronidase (gus) genes, was utilized in these experiments. Somatic embryos, embryogenic calluses, mature caryopses, and plantlet segments served as target tissues for infection. Treated cultures were selected in the presence of 10 mg L−1 bialaphos and the resultant plantlets were treated with the herbicide Basta [monoammonium 2-amino-4(hydroxymethylphosphinyl)butanoate]. T-DNA delivery efficiency was affected by genotype, explant used and the presence or absence of acetosyringone (3′,5′-dimethoxy-4′-hydroxyacetophenone) during ...
Steviol glycosides will be the sweet compounds for the leaves of this stevia plant. Each one is composed of a backbone device of steviol, with varying numbers/configurations of sugar units connected, particular...
Thermoanaerobacter ethanolicus produces a considerable amount of ethanol from a range of carbohydrates and is an attractive candidate for applications in bioconversion processes. A genetic system with reusable selective markers would be useful for deleting acid production pathways as well as other genetic modifications. The thymidine kinase (tdk) gene was deleted from T. ethanolicus JW200 to allow it to be used as a selectable marker, resulting in strain X20. Deletion of the tdk gene reduced growth rate by 20 %; however, this could be reversed by reintroducing the tdk gene (strain X20C). The tdk and high-temperature kanamycin (htk) markers were tested by using them to delete lactate dehydrogenase (ldh). During positive selection of ldh knockouts in strain X20 on kanamycin agar plates, six out of seven picked colonies were verified transformants. Deletion of ldh reduced lactic acid production by 90 %. The tdk and 5-fluoro-2′-deoxyuridine (FUDR) combination worked reliably as
Transformation efficiency is the efficiency by which cells can take up extracellular DNA and express genes encoded by it. This is based on the competence of the cells. It can be calculated by dividing the number of successful transformants by the amount of DNA used during a transformation procedure. Transformants are cells that have taken up DNA (foreign, artificial or modified) and which can express genes on the introduced DNA. Transformation efficiency should be determined under conditions of cell excess. The number of viable cells in a preparation for a transformation reaction may range from 2×108 to 1011; most common methods of E. coli preparation yield around 1010 viable cells per reaction. The standard plasmids used for determination of transformation efficiency in Escherichia coli are pBR322 or other similarly-sized or smaller vectors, such as the pUC series of vectors. Different vectors however may be used to determine their transformation efficiency. 10-100 pg of DNA may be used for ...
44,203-229. , Hirsch, P. , Hooykaas, P J J , and Schilperoort, R. A (1983) A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Tr-plasmrd. Nature 303, 179,180. 8. Stachel, S. E and Nester, E W. (1986) The genetic and transcriptional organization of the vir region of the A6 TI plasmid of Agrobacterium tumefaciens. EMBO J. $1445-1454 9. Stachel, S E and Zambryski, P (1986) virA and virG control the plant-induced activation of the T-DNA transfer process of Agrobacterium tumefaciens. Biol. Rep. 10, 12-36. &APTER 7 Leaf Disk Transformation Ian S. Curtis, Michael R. Davey, and J. Brian Power 1. Introduction Reliable and efficient methods of transferring cloned genes into plants are essential for engineering crops with desired traits. The Gram-negative soil bacteria, Agrobacterium tumefaciens and A. rhizogenes, are natural genetic engineers, capable of transforming a range of dicotyledonous plants by transferring plasmid-encoded genes into recipient ...
Rhizobium rhizogenes (formerly Agrobacterium rhizogenes) is a Gram-negative soil bacterium that produces hairy root disease in dicotyledonous plants. R. rhizogenes induces the formation of proliferative multiple-branched adventitious roots at the site of infection, so-called hairy roots. In the rhizosphere, plants may suffer from wounds by soil pathogens or other sources. This leads to the secretion of phenolic compounds like acetosyringone which have chemotactic effects that attract the bacteria. Under such conditions, certain bacterial genes are turned on leading to the transfer of its tDNA from its root-inducing plasmid (Ri plasmid) into the plant through the wound. After integration and expression, in vitro or under natural conditions, the hairy root phenotype is observed, which typically includes overdevelopment of a root system that is not completely geotropic, and altered (wrinkled) leaf morphology, if leaves are present. Bacterial genes may be retained within the plant. The hairy roots ...
On December 18, 2008, the Food and Drug Administration ruled the natural sweetener Truvia "generally safe" for use in foods and beverages. Truvia (trade name Rebiana) is comprised of a diterpene called steviol glycoside, which is isolated from the extracts of the leaves of the plant Stevia rebaudiana bertoni. The herb Stevia-basically the leaves of the plant-has been available for years, so steviol glycoside is nothing new, per se. Cargill Food and Ingredient Systems now markets Truvia as a singular, "fully-characterized product" (Stevia, by comparison is a witchs brew of anywhere from 40 to 200 compounds). More interesting data about Truvia can be found here. As a result of the FDAs ruling, Coca-Cola Company will soon launch a new line of reduced-calorie drinks with the most prominent being a new version of Sprite called Sprite Green (comes in a nifty aluminum can). Stevia was purportedly discovered by Moises Santiago Bertoni in 1887 while exploring the forests of Paraguay-Stevia rebaudianas ...
Weiler Nutrition Communications Inc. is a Toronto based consulting practice which focuses on strategic marketing, nutrition education and regulatory affairs related to food and beverages.
A commonly encountered difficulty with the genetic engineering of crop plants is that different varieties of a particular species can show great variability in the efficiency with which they can be transformed. This increases the effort required to introduce transgenes into particular genetic backgrounds. The use of Substitution Lines has allowed the finer mapping of three Quantitative Trait Loci (tf1, tf2 and tf3) that explain 26% of the variation in the efficiency of Agrobacterium-mediated transformation in Brassica oleracea. Use of an orthogonal set of genotypes (containing all eight possible combinations of positive and negative alleles at the three QTL), along with time course studies of transgene expression, has allowed the determination of the stages at which these genes have their effects during transformation. With regard to control of the level of transient transgene expression, tf1 (on LGO1) alone has no detectable effect, whilst tf2 (on LGO3) and tf3 (on LGO7) have highly significant
Multiporator / Electroporator 2510 Transformation Protocol Protocol No. 4308 915.513 04/2002 Microorganism Escherichia coli DH10B,Escherichia,coli,DH10B,biological,advanced biology technology,biology laboratory technology,biology device technology,latest biology technology
ALIPPI, Adriana M; LOPEZ, Ana C y BALATTI, Pedro A. Methods for the detection of Agrobacterium from plant, soil and water samples. Rev. argent. microbiol. [online]. 2011, vol.43, n.4, pp. 278-286. ISSN 0325-7541.. The genus Agrobacterium includes phytopathogenic bacteria that induce the development of root crown galls and/or aerial galls at the base of the stem or hairy roots on more than 600 species of plants belonging to 90 dicotyledonous families and non-pathogenic species. These bacteria being natural soil inhabitants are particularly diffcult to eradicate, which is a problem in nurseries where more than 80% of infections occur. Since early detection is crucial to avoid the inadvertent spread of the disease, the aim of this work was to develop sensitive and precise identifcation techniques by using a set of semi-selective and differential culture media in combination with a specifc PCR to amplify a partial sequence derived from the virC operon, as well as a multiplex PCR on the basis of ...
... - A generation ago, many Indians, especially in the southern part of the country, were familiar with ragi or finger millet (Eleusine coracana L.). The once well-known cereal is however totally absent in...
Sina Shamsavari is an artist, cartoonist and zinester. In 1993 Sina created and self-published the zine Concerned Müthers, aimed at "queer teens and their strait mates". Seven issues of the zine were released, until Sina ceased publication in 1995. This was quickly followed by the highly-acclaimed, primarily autobiographical BoyCrazyBoy. Sina has also contributed to a number of zines, including Boy Trouble. His work can regularly be seen in the anthology The Book of Boy Trouble Volumes 1 and 2. ...
Sina Sabet, MD specializes in Ophthalmology at the Inova Health System. Learn more about this providers specialty, hospital affiliation, practice locations and how to request an appointment.
Rhizobium, Agrobacterium and Allorhizobium are genera within the bacterial family Rhizobiaceae, together with Sinorhizobium. The species of Agrobacterium, Agrobacterium tumefaciens (syn. Agrobacterium radiobacter), Agrobacterium rhizogenes, Agrobacterium rubi and Agrobacterium vitis, together with Allorhizobium undicola, form a monophyletic group with all Rhizobium species, based on comparative 16S rDNA analyses. Agrobacterium is an artificial genus comprising plant-pathogenic species. The monophyletic nature of Agrobacterium, Allorhizobium and Rhizobium and their common phenotypic generic circumscription support their amalgamation into a single genus, Rhizobium. Agrobacterium tumefaciens was conserved as the type species of Agrobacterium, but the epithet radiobacter would take precedence as Rhizobium radiobacter in the revised genus. The proposed new combinations are Rhizobium radiobacter, Rhizobium rhizogenes, Rhizobium rubi, Rhizobium undicola and Rhizobium vitis.
The study was conducted at the In Vitro Laboratory, Institut Pertanian Persekitaran Terkawal (IPPT), Faculty of Agriculture and Molecular and Cell Biology Laboratory, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor. The main objectives of the study were to develop in-vitro plantlet regeneration systems and to carry out preliminary studies of Agrobacterium-mediated gene transformation of Carica papaya L. cv. Eksotika. The study consisted of in-vitro plantlet regeneration through organogenesis, and somatic embryogenesis using different types of explant and different concentrations and combinations of plant growth regulators whereby the most appropriate system could be applied in the genetic transformation of the crop. The experiments were arranged in a completely randomized design (CRD). The study on organogenesis included shoot induction and rooting. On shoot induction, modified MS (Murashige and Skoog, 1962) medium supplemented with 500 mgL-1 ...
As a special phytopathogen, Agrobacterium tumefaciens infects a wide range of plant hosts and causes plant tumors also known as crown galls. The complexity of Agrobacterium-plant interaction has been studied for several decades. Agrobacterium pathogenicity is largely attributed to its evolved capabilities of precise recognition and response to plant-derived chemical signals. Agrobacterium perceives plant-derived signals to activate its virulence genes, which are responsible for transferring and integrating its Transferred DNA (T-DNA) from its Tumor-inducing (Ti) plasmid into the plant nucleus. The expression of T-DNA in plant hosts leads to the production of a large amount of indole-3-acetic acid (IAA), cytokinin (CK), and opines. IAA and CK stimulate plant growth, resulting in tumor formation. Agrobacterium utilizes opines as nutrient sources as well as signals in order to activate its quorum sensing (QS) to further promote virulence and opine metabolism. Intriguingly, Agrobacterium also ...
Executing: lynx Lambda lunch update: 3/4/13 3/4/13: Monday, 4:00 PM, Bldg. 4, Rm. 433: Stanton Gelvin (Purdue University): "Enhancing Agrobacterium-Mediated Genetic Transformation by Manipulating the Host Genome" (David Margulies, [1][email protected]) 3/7/13*: Ding Jin "Role of RNA Polymerase in Organization of E. coli Nucleoid " 3/14/13*: Eric Cambronne (Oregon Health and Science University) "Effector protein complexes that prime Legionella pneumophila for human infection" (K. Ramamurthi) 3/21/13*: Aneeshkumar Arimbasseri (NICHD, Maraia lab) "Distinguishing core and holoenzyme mechanisms of transcription termination by RNA polymerase III" 3/28/13* Chase Beisel (North Carolina State Univ.) "Bacterial sugar utilization at the single-cell level" 4/4/13*: Matthias Machner 4/18/13*: Amy Vollmer (Swarthmore College) (Gigi Storz) 4/25/13*: Orna Cohen-Fix 5/1/13*: Jeffrey Gardner (UMBC) (Not the lambda recombination J. Gardner; soil bacteria sensing the environment) 6/26/13: 3:00 PM Masur Auditorium (WALS): ...
Calcium is key for growth and we need plenty of it in our daily food from a very young age. Yet, about half the global population, mostly in Asia and Africa, lack calcium in their diet and are prone to many related ailments ranging from cardiovascular diseases and diabetes to bone loss, which leads to crippling osteoporosis at old age.. Scientists from Aberystwyth University, UK and the International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) recommend biofortifying finger millet, an already calcium-rich dryland cereal grown in India and Africa, to combat this significant micronutrient deficiency.. One woman out of three and one man out of five will be exposed to bone loss and related fractures during their lives and the societal cost is rising fast, both in developed and developing countries. Think of a bone lifesaving account. Children need to get as much calcium as possible during their childhood to prevent osteoporosis which is very difficult to detect at an early stage. In ...
Tytuł projektu: Rozbudowa i przekształcenie bibliograficznej bazy danych AGRO w bazę bibliograficzno-abstraktową z wykorzystaniem oprogramowania YADDA. Nr umowy: POIG 02.03.02-00-031/09 (okres realizacji 2009-2013 ...
295393009 - EP 1032693 A1 2000-09-06 - A METHOD FOR DIRECTIONAL STABLE TRANSFORMATION OF EUKARYOTIC CELLS - [origin: US6187994B1] Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.[origin: US6187994B1] Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange
AGROBACTERIUM :: Information about AGROBACTERIUM -- FindTheWord.info is a search engine for English words. FindTheWord.info searches for partial words (both crossword solver and part of word), help with cheating in Scrabble and Wordfeud, finds anagrams, palindromes, and words in word, and much more.The dictionary used contains more than 589,000 English words.
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GM walnuts could soon hit the supermarket shelves, after scientists at the University of California developed a way to modify the roots of the walnut tree to enable them to fight infection from a bug called Agrobacterium. Agrobacterium causes cancerous growth that invades the plants DNA.
Methods for evolving recombinase protein homologues and RecA/VirE2 fusion proteins which complement VirE2 deficient Agrobacterium are provided. The use of recombinase protein homologues and RecA/VirE2 fusion proteins in the context of Agrobacterium mediated transformation are provided. Methods for producing transgenic organisms by homologous recombination using evolved recombinase proteins and Agrobacterium strains which express recombinase protein homologues or RecA/VirE2 fusion proteins are provided. Transgenic cells and organisms which have integrated an exogenous DNA sequence into a predetermined site in their genome are provided.
There are 20 calories in 1 tablespoon (0.6 oz) of Smuckers Squeeze Reduced Sugar Strawberry Fruit Spread. Youd need to walk 5 minutes to burn 20 calories. Visit CalorieKing to see calorie count and nutrient data for all portion sizes.
Many bacteria, including Agrobacterium tumefaciens, rely on an asymmetric localization, distribution, and orientation of specific appendages when interacting wi...
Octopine and nopaline strains of Agrobacterium tumefaciens differ in their ability to induce tumors on Nicotiana glauca. The presence of a virF locus on the octopine Ti plasmid makes N. glauca a host plant for these strains, indicating that the VirF protein is a host-range determinant. Here we show the presence of a virF locus not only on the Agrobacterium vitis octopine/cucumopine plasmids pTiAg57 and pTiTm4, but also on the nopaline Ti plasmids pTiAT1, pTiAT66a, and pTiAT66b. On the octopine Ti plasmids from A. tumefaciens the virF gene is located between the virE locus and the left border of the T-region. In contrast, the virF gene on Ti plasmids of A. vitis is located at the very left end of the vir-region near the virA locus. The virF gene of pTiAg57 has been sequenced and codes for a protein of 202 amino acids with a molecular mass of 22,280 Da. Comparison showed that the virF gene from A. vitis strain Ag57 is almost identical to that from A. tumefaciens octopine strains. The transcription of the
Transgenic trees currently are being produced by Agrobacterium-mediated transformation and biolistics. Since trees are particularly suited for long-term evaluations of the impact of the technology, Prunus subhirtella autumnorosa (PAR) was chosen as model fruit tree species and transformed with a reporter gene (uidA) under the control of the 35S promoter. Using Southern and GUS fluorometric techniques, we compared transgene copy numbers and observed stability of transgene expression levels in 34 different transgenic plants, grown under in vitro, greenhouse and screenhouse conditions, over a period of 9 years. An influence of grafting on gene expression was not observed. No silenced transgenic plant was detected. Overall, these results suggest that transgene expression in perennial species, such as fruit trees, remains stable in time and space, over extended periods and in different organs, confirming the value of PAR as model species to study season-dependent regulation in mature stonefruit ...
The parental MON 89034 has the expression cassettes of genes Cry1A.105 and cry2Ab 2 (derived from Bacillus thuringiensis), which encode the proteins Cry1A.105 and Cry2Ab2, respectively, responsible for insect resistance. The parental NK 603 maize contains two expression cassettes of the cp4 epsps gene (derived from Agrobacterium sp. CP4 strain), with the respective regulatory sequence. The cp4 epsps gene encodes the protein 5-enolpyruvyl-shikimate-3-phosphate synthesis (CP4 EPSPS), conferring tolerance to the herbicide glyphosate. The pyramided parental maize MON 89034 x NK 603 has been extensively tested, and its biosafety been examined in separate cases, both approved by CTNBio. The proteins present in NK 603 x MON89034 maize have a history of safe use by the consumption of MON 89034 maize since 2008, and the NK603 maize since 2000. The MON 89034 x NK 603 maize is approved in Japan, North Korea, the Philippines, Taiwan, and the USA. Concerning the proteins expressed by MON 89034 x NK 603 ...
Stevia (Stevia rebaudiana) is a perennial herbaceous plant native to Paraguay and used traditionally by the native Guarani peoples for centuries to sweeten medicinal teas. Stevia has only been commercially cultivated over the past 50 years and cultural practice information suitable for implementation by growers is limited. Currently stevias commercial value is found in its steviol glycoside content, especially rebaudioside A. Growers seek information to maximize productivity of rebaudioside A through improved cultural practices. Irrigation management is one of several important agronomic techniques growers can use to obtain maximum yield while also pursuing water use efficiency. The current trial investigated the effect that soil water tension as an irrigation onset criteria had on stevia dry leaf yields, steviol glycoside content and yield, and steviol glycoside ratios. The experiment was conducted in a field of Owyhee silt loam (coarse-silty, mixed, mesic Xerollic Camborthid) at the Oregon ...
Elicitation with middle-viscous chitosan (30 mg/50 mL) significantly stimulated silymarin synthesis in Silybum marianum hairy root cultures. The root cultures established by infection with Agrobacterium rhizogenes AR15834 showed a potential for production of silymarin. Elicitation with medium molecular weight of chitosan (0, 5, 10, 20, and 30 mg/50 mL) was used in order to improve silymarin production. Total silymarin increased about 5.26-fold after 96 h of treatment with 30 mg/50 mL chitosan. Dry weight of the hairy roots reached the highest point (0.530 and 0.535 g) after 96 h in presence of 20 and 30 mg/50 mL chitosan, respectively. Five different flavonolignans were isolated; taxifolin, silychristin, silydianin, silybin and isosilybin) 0.133, 0.200, 0.120, 0.041 and 0.056 mg/g dry weight, respectively). 30 days old hairy roots were treated by 30 mg/50 mL chitosan in different times (12, 24, 48, 72, 96 and 120 h). The amount of silymarin accumulation significantly increased (0.705 mg/gDW) in hairy

Agrobacterium - WikipediaAgrobacterium - Wikipedia

Agrobacterium rhizogenes, and Agrobacterium vitis. Strains within Agrobacterium tumefaciens and Agrobacterium rhizogenes are ... "Proposal for Rejection of Agrobacterium tumefaciens and Revised Descriptions for the Genus Agrobacterium and for Agrobacterium ... Agrobacterium tumefaciens is the most commonly studied species in this genus. Agrobacterium is well known for its ability to ... The Agrobacterium genus is quite heterogeneous. Recent taxonomic studies have reclassified all of the Agrobacterium species ...
more infohttps://en.wikipedia.org/wiki/Agrobacterium

Agrobacterium albertimagni - WikipediaAgrobacterium albertimagni - Wikipedia

Agrobacterium albertimagni is a species of arsenite-oxidizing bacterium. Salmassi, Tina M.; Venkateswaren, Kasthuri; Satomi, ... Trimble WL, Phung le T, Meyer F, Gilbert JA, Silver S (2012). "Draft genome sequence of Agrobacterium albertimagni strain AOL15 ... Agrobacterium albertimagni at the Encyclopedia of Life LPSN. ... "Oxidation of Arsenite by Agrobacterium albertimagni , AOL15, sp ...
more infohttps://en.wikipedia.org/wiki/Agrobacterium_albertimagni

Agrobacterium problemsAgrobacterium problems

... Steve Wylie wylie at CENTRAL.MURDOCH.EDU.AU Tue Oct 10 21:20:46 EST 1995 *Previous message: Geneclean ... Does anyone else working with Agrobacterium tumefaciens have the problems that I do? Firstly, it is very inconsistant in growth ...
more infohttp://www.bio.net/bionet/mm/methods/1995-October/034686.html

Agrobacterium strainsAgrobacterium strains

Now I want the blank cultures of Agrobacterium , strains, LBA 4404, EHA 105 and EHA 101 so that I can introduce my vector , ... Agrobacterium strains. Dr Engelbert Buxbaum engelbert_buxbaum at hotmail.com Thu Jun 8 07:13:43 EST 2006 *Previous message: How ... Can you please , tell me how to get these Agrobacterium strains. There are microbiological type culture collection, for example ...
more infohttp://www.bio.net/bionet/mm/methods/2006-June/100686.html

Agrobacterium tumefaciens C58 Genome & Functional Genomics ProjectAgrobacterium tumefaciens C58 Genome & Functional Genomics Project

Brief Description of Agrobacterium tumefaciens C58:. A. tumefaciens C58 is the most heavily studied member of the genus ... Scientists know a lot now about virulent Agrobacterium strains do this and have worked out methods to use this natural DNA ... However, most people know Agrobacterium because some strains can do something that no other cellular pathogen does - inject a ... Agrobacterium. Members of this genus are commonly found in soils all over the world as a aerobic heterotroph that can use a ...
more infohttps://sites.google.com/site/goodneragro/

KEGG GENOME: Agrobacterium fabrumKEGG GENOME: Agrobacterium fabrum

Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium; ... Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58. ...
more infohttp://www.genome.jp/dbget-bin/www_bget?gn:T00070

MULTISPECIES: hydantoinase/oxoprolinase family protein [Agrobacterium] - Protein - NCBIMULTISPECIES: hydantoinase/oxoprolinase family protein [Agrobacterium] - Protein - NCBI

MULTISPECIES: hydantoinase/oxoprolinase family protein [Agrobacterium]. NCBI Reference Sequence: WP_012649231.1. Identical ... MULTISPECIES: hydantoinase/oxoprolinase family protein [Agrobacterium] MULTISPECIES: hydantoinase/oxoprolinase family protein [ ...
more infohttps://www.ncbi.nlm.nih.gov/protein/WP_012649231.1

Agrobacterium: From Biology to Biotechnology | SpringerLinkAgrobacterium: From Biology to Biotechnology | SpringerLink

Agrobacterium is the only cellular organism on Earth that is naturally capable of transferring genetic material between the ... The Oncogenes of Agrobacterium Tumefaciens and Agrobacterium Rhizogenes Monica T. Britton, Matthew A. Escobar, Abhaya M. ... Agrobacterium is a comprehensive book on Agrobacterium research, including its history, application, basic biology discoveries ... Agrobacterium DNA Expression Pathogen Pathogene Transport biotechnology crown gall disease currentjks gene transfer genetic ...
more infohttps://link.springer.com/book/10.1007%2F978-0-387-72290-0

Accomodation | AgrobacteriumAccomodation | Agrobacterium

Ghent has a wide range of accommodations: from hotels, over B&B to youth hostels. Below, we have made some suggestions for nice hotels near the Gent Sint-Pieters railway station, which you can easily reach when arriving by train, and from which you can easily go to the conference venue in Zwijnaarde by bus. ...
more infohttps://www.psb.ugent.be/agro/accomodation

The T-pilus of Agrobacterium tumefaciens.  - PubMed - NCBIThe T-pilus of Agrobacterium tumefaciens. - PubMed - NCBI

The T-pilus of Agrobacterium tumefaciens.. Lai EM1, Kado CI.. Author information. 1. Davis Crown Gall Group, University of ... and protein-transport apparatus for the transport of cyclic T-pilin subunits to the Agrobacterium cell surface. T-pilin ... subunits are processed from full-length VirB2 pro-pilin into a cyclized peptide, a rapid reaction that is Agrobacterium ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/10920395?dopt=Abstract

Plant Viral Vectors for Delivery by Agrobacterium | SpringerLinkPlant Viral Vectors for Delivery by Agrobacterium | SpringerLink

Gelvin SB (2012) Traversing the cell: Agrobacterium T-DNAs journey to the host genome. Front Plant Sci 3:52PubMedGoogle ... Gelvin SB (2008) Agrobacterium-mediated DNA transfer, and then some. Nat Biotechnol 26:998-1000PubMedGoogle Scholar ... The strategy relies on Agrobacterium as a vector to deliver DNA copies of one or more viral RNA/DNA replicons; the bacteria are ... Magori S, Citovsky V (2011) Epigenetic control of Agrobacterium T-DNA integration. Biochim Biophys Acta 1809:388-394PubMed ...
more infohttps://link.springer.com/chapter/10.1007%2F82_2013_352

Agrobacterium radiobacter (strain K84 / ATCC BAA-868)Agrobacterium radiobacter (strain K84 / ATCC BAA-868)

This proteome is part of the Agrobacterium radiobacter pan proteome (fasta) Agrobacterium are Gram-negative, motile, soil- ... More recently Agrobacterium have been classified into 3 biovars based on physiological and biochemical phenotypes without ... "Genome sequences of three Agrobacterium biovars help elucidate the evolution of multichromosome genomes in bacteria.". Slater S ...
more infohttp://www.uniprot.org/proteomes/UP000001600

Cytokinin production by Agrobacterium tumefaciensCytokinin production by Agrobacterium tumefaciens

Certain plant-associated prokaryotes such as Corynebacteriurn fascians and Agrobacterium tumefaciens are known to produce ... Cytokinin production by Agrobacterium tumefaciens Public Deposited Citeable URL:. http://ir.library.oregonstate.edu/concern/ ...
more infohttps://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/6t053k05z

Agrobacterium tumafaciens | Wards ScienceAgrobacterium tumafaciens | Ward's Science

Agrobacterium tumefaciens Nutrient Agar. 30°C. Refrigerator. Tube. Each. Retrieving. This Item is temperature sensitive and has ... A. tumefaciens is also known as Rhizobium radiobacter, and Agrobacterium radiobacter.. All microbial cultures undergo an ...
more infohttps://www.wardsci.com/store/product/8865860/agrobacterium-tumafaciens

Agrobacterium Transformation Kits - MP BiomedicalsAgrobacterium Transformation Kits - MP Biomedicals

Unless specified otherwise, MP Biomedicals products are for laboratory research use only, not for human or clinical use. For more information, please contact our customer service department ...
more infohttps://www.mpbio.com/index.php?cPath=2873_2_2000_2006_2041&country=223

Agrobacterium Transformation Kits - MP BiomedicalsAgrobacterium Transformation Kits - MP Biomedicals

Unless specified otherwise, MP Biomedicals products are for laboratory research use only, not for human or clinical use. For more information, please contact our customer service department ...
more infohttps://www.mpbio.com/index.php?cPath=2_2000_2006_2041&country=223

KEGG PATHWAY: Nucleotide excision repair - Agrobacterium fabrumKEGG PATHWAY: Nucleotide excision repair - Agrobacterium fabrum

Nucleotide excision repair - Agrobacterium fabrum [ Pathway menu , Organism menu , Pathway entry , Download KGML , Show ...
more infohttp://www.genome.jp/kegg-bin/show_pathway?atu03420

Agrobacterium Biology - From Basic Science to Biotechnology | Stanton B. Gelvin | SpringerAgrobacterium Biology - From Basic Science to Biotechnology | Stanton B. Gelvin | Springer

This volume reviews various facets of Agrobacterium biology, from modern aspects of taxonomy and bacterial ecology to ... Agrobacterium Biology. Book Subtitle. From Basic Science to Biotechnology. Editors. * Stanton B. Gelvin ... Agrobacterium Biology. From Basic Science to Biotechnology. Editors: Gelvin, Stanton B. (Ed.) ... Agrobacterium-mediated transformation is the most extensively utilized platform for generating transgenic plants, but modern ...
more infohttps://www.springer.com/gp/book/9783030032562

Agrobacterium tumefaciens electrocompetent cellsAgrobacterium tumefaciens electrocompetent cells

... for T-DNA transfer to plant cells and preparation of transgenic recombinant ... Agrobacterium tumefaciens LBA4404 Electro-Cells. 5 x 40 uL. $259.00 Agrobacterium tumefaciens (Rhizobium radiobactor) can ... Agrobacterium tumefaciens electrocompetent cells. Agrobacterium tumefaciens (Rhizobium radiobactor) is capable of T-DNA ... Agrobacterium tumefaciens (Rhizobium radiobactor) is capable of T-DNA transfer to plant cells. The T-DNA (transfer DNA) is ...
more infohttps://cellartis.com/products/cloning/competent-cells/agrobacterium-electrocompetent-cells

Plasmid isolation from agrobacterium - -Genetics and EpigeneticsPlasmid isolation from agrobacterium - -Genetics and Epigenetics

Plasmid isolation from agrobacterium - (Feb/04/2013 ). Hi, anyone here knows the protocol to isolate the binary vector from ... resources/articles/pubhub/promega-notes-1999/wizard-plus-minipreps-for-the-isolation-of-binary-plasmids-from-agrobacterium- ...
more infohttp://www.protocol-online.org/biology-forums-2/posts/28376.html

Agrobacterium tumefaciens-mediated Transformation of Plant Cells - eLS - Binns - Wiley Online LibraryAgrobacterium tumefaciens-mediated Transformation of Plant Cells - eLS - Binns - Wiley Online Library

Agrobacterium tumefaciens is a Gram-negative soil bacterium that causes plant tumours by transferring a portion of DNA from a ...
more infohttp://onlinelibrary.wiley.com/doi/10.1038/npg.els.0001492/abstract

Agrobacterium radiobacter | Tabers Medical DictionaryAgrobacterium radiobacter | Taber's Medical Dictionary

Agrobacterium radiobacter answers are found in the Tabers Medical Dictionary powered by Unbound Medicine. Available for iPhone ... Agrobacterium radiobacter is a sample topic from the Tabers Medical Dictionary. To view other topics, please sign in or ... Tabers Medical Dictionary [Internet]. Agrobacterium radiobacter; Tabers Online. [cited 2018 August 14]; Available from: https ... www.unboundmedicine.com/medline/view/Tabers-Dictionary/741040/all/Agrobacterium_radiobacter. Accessed 14 August 2018. ...
more infohttps://www.tabers.com/tabersonline/view/Tabers-Dictionary/741040/all/Agrobacterium_radiobacter

Sequncing result for agrobacterium plasmidSequncing result for agrobacterium plasmid

... By Home › Forums › DNA Sequencing Forum › Sequncing result for agrobacterium ... Previously I cloned my sequence into agrobacterium plasmid as later on I need to use it to infect my plants. But when I ... extracted the agrobacterium plasmid and sent it for sequencing, the result was terrible. The technician told me it was due to ...
more infohttps://www.nucleics.com/forums/topic/sequncing-result-for-agrobacterium-plasmid/

Regeneration and Agrobacterium-mediated transformation of multiple lily cultivarsRegeneration and Agrobacterium-mediated transformation of multiple lily cultivars

... Author(s). Wang, Yue; Kronenburg-van de Ven ... Calli of eight randomly chosen cultivars were co-cultivated with Agrobacterium tumefaciens strain AGL0 carrying binary vectors ...
more infohttp://library.wur.nl/WebQuery/wurpubs/428457

Frontiers | Agrobacterium tumefaciens responses to plant-derived signaling molecules | Plant ScienceFrontiers | Agrobacterium tumefaciens responses to plant-derived signaling molecules | Plant Science

The complexity of Agrobacterium-plant interaction has been studied for several decades. Agrobacterium pathogenicity is largely ... The complexity of Agrobacterium-plant interaction has been studied for several decades. Agrobacterium pathogenicity is largely ... Here we outline the responses of Agrobacterium to major plant-derived signals that impact Agrobacterium-plant interactions. ... Agrobacterium utilizes opines as nutrient sources as well as signals in order to activate its quorum sensing (QS) to further ...
more infohttps://www.frontiersin.org/articles/10.3389/fpls.2014.00322/full
  • In roots as well as in shoots, Agrobacterium tumefaciens and E. coli triggered similar (in PR37Y15) or different (in DK315) changes in the high-performance liquid chromatography profiles of secondary metabolites (especially benzoxazinoids), distinct from those of Azospirillum brasilense UAP-154. (apsnet.org)
  • Agrobacterium pathogenicity is largely attributed to its evolved capabilities of precise recognition and response to plant-derived chemical signals. (frontiersin.org)