A species of gram-negative, aerobic bacteria isolated from soil and the stems, leafs, and roots of plants. Some biotypes are pathogenic and cause the formation of PLANT TUMORS in a wide variety of higher plants. The species is a major research tool in biotechnology.
A genus of gram-negative, aerobic, rod-shaped bacteria that activate PLANT ROOT NODULATION in leguminous plants. Members of this genus are nitrogen-fixing and common soil inhabitants.
A genus of gram negative, aerobic, rod-shaped bacteria found in soil, plants, and marine mud.
A localized proliferation of plant tissue forming a swelling or outgrowth, commonly with a characteristic shape and unlike any organ of the normal plant. Plant tumors or galls usually form in response to the action of a pathogen or a pest. (Holliday, P., A Dictionary of Plant Pathology, 1989, p330)
Plasmids coding for proteins which induce PLANT TUMORS. The most notable example of a plant tumor inducing plasmid is the Ti plasmid found associated with AGROBACTERIUM TUMEFACIENS.
Change brought about to an organisms genetic composition by unidirectional transfer (TRANSFECTION; TRANSDUCTION, GENETIC; CONJUGATION, GENETIC, etc.) and incorporation of foreign DNA into prokaryotic or eukaryotic cells by recombination of part or all of that DNA into the cell's genome.
Proteins found in any species of bacterium.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
Those components of an organism that determine its capacity to cause disease but are not required for its viability per se. Two classes have been characterized: TOXINS, BIOLOGICAL and surface adhesion molecules that effect the ability of the microorganism to invade and colonize a host. (From Davis et al., Microbiology, 4th ed. p486)
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
Multicellular, eukaryotic life forms of kingdom Plantae (sensu lato), comprising the VIRIDIPLANTAE; RHODOPHYTA; and GLAUCOPHYTA; all of which acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations.
The functional hereditary units of BACTERIA.
A plant genus of the family CRASSULACEAE. Members contain bryophyllins (also called bryotoxins) which are bufadienolides (BUFANOLIDES) that have insecticidal activity.
PLANTS, or their progeny, whose GENOME has been altered by GENETIC ENGINEERING.
A family of gram-negative bacteria which are saprophytes, symbionts, or plant pathogens.
A plant genus of the family SOLANACEAE. Members contain NICOTINE and other biologically active chemicals; its dried leaves are used for SMOKING.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
An aminopurine factor in plant extracts that induces cell division. (Grant & Hackh's Chemical Dict, 5th ed)
The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.
Plants or plant parts which are harmful to man or other animals.
Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.
A parasexual process in BACTERIA; ALGAE; FUNGI; and ciliate EUKARYOTA for achieving exchange of chromosome material during fusion of two cells. In bacteria, this is a uni-directional transfer of genetic material; in protozoa it is a bi-directional exchange. In algae and fungi, it is a form of sexual reproduction, with the union of male and female gametes.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Diseases of plants.
One of the FURANS with a carbonyl thereby forming a cyclic lactone. It is an endogenous compound made from gamma-aminobutyrate and is the precursor of gamma-hydroxybutyrate. It is also used as a pharmacological agent and solvent.
A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The usually underground portions of a plant that serve as support, store food, and through which water and mineral nutrients enter the plant. (From American Heritage Dictionary, 1982; Concise Dictionary of Biology, 1990)
An exocellulase with specificity for a variety of beta-D-glycoside substrates. It catalyzes the hydrolysis of terminal non-reducing residues in beta-D-glucosides with release of GLUCOSE.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Structures within the nucleus of bacterial cells consisting of or containing DNA, which carry genetic information essential to the cell.
A species of gram-negative, aerobic bacteria that causes formation of root nodules on some, but not all, types of sweet clover, MEDICAGO SATIVA, and fenugreek.
Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene or extragenic sequence. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA insertions into or adjacent to a cellular proto-oncogene can interrupt GENETIC TRANSLATION of the coding sequences or interfere with recognition of regulatory elements and cause unregulated expression of the proto-oncogene resulting in tumor formation.
An essential amino acid that is physiologically active in the L-form.
In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
A plant species of the genus DATURA, family SOLANACEAE, that contains TROPANES and other SOLANACEOUS ALKALOIDS.
Aminoglycoside produced by Streptomyces hygroscopicus. It is used as an anthelmintic against swine infections by large roundworms, nodular worms, and whipworms.
The process of embryo initiation in culture from vegetative, non-gametic, sporophytic, or somatic plant cells.
Plant hormones that promote the separation of daughter cells after mitotic division of a parent cell. Frequently they are purine derivatives.
Expanded structures, usually green, of vascular plants, characteristically consisting of a bladelike expansion attached to a stem, and functioning as the principal organ of photosynthesis and transpiration. (American Heritage Dictionary, 2d ed)
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
Glucose polymers consisting of a backbone of beta(1->3)-linked beta-D-glucopyranosyl units with beta(1->6) linked side chains of various lengths. They are a major component of the CELL WALL of organisms and of soluble DIETARY FIBER.
A species of gram-negative bacteria, primarily infecting SWINE, but it can also infect humans, DOGS, and HARES.
A single chain of deoxyribonucleotides that occurs in some bacteria and viruses. It usually exists as a covalently closed circle.
The large family of plants characterized by pods. Some are edible and some cause LATHYRISM or FAVISM and other forms of poisoning. Other species yield useful materials like gums from ACACIA and various LECTINS like PHYTOHEMAGGLUTININS from PHASEOLUS. Many of them harbor NITROGEN FIXATION bacteria on their roots. Many but not all species of "beans" belong to this family.
A plant genus of the family BRASSICACEAE that contains ARABIDOPSIS PROTEINS and MADS DOMAIN PROTEINS. The species A. thaliana is used for experiments in classical plant genetics as well as molecular genetic studies in plant physiology, biochemistry, and development.
A class of enzymes that catalyze the hydrolysis of one of the three ester bonds in a phosphotriester-containing compound.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
A diuretic and renal diagnostic aid related to sorbitol. It has little significant energy value as it is largely eliminated from the body before any metabolism can take place. It can be used to treat oliguria associated with kidney failure or other manifestations of inadequate renal function and has been used for determination of glomerular filtration rate. Mannitol is also commonly used as a research tool in cell biological studies, usually to control osmolarity.
A phenomenon where microorganisms communicate and coordinate their behavior by the accumulation of signaling molecules. A reaction occurs when a substance accumulates to a sufficient concentration. This is most commonly seen in bacteria.
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
An enzyme that catalyzes the oxidation of protocatechuate to 3-carboxy-cis-cis-muconate in the presence of molecular oxygen. It contains ferric ion. EC 1.13.11.3.
Polysaccharides composed of repeating glucose units. They can consist of branched or unbranched chains in any linkages.
A class in the phylum PROTEOBACTERIA comprised mostly of two major phenotypes: purple non-sulfur bacteria and aerobic bacteriochlorophyll-containing bacteria.
The heritable modification of the properties of a competent bacterium by naked DNA from another source. The uptake of naked DNA is a naturally occuring phenomenon in some bacteria. It is often used as a GENE TRANSFER TECHNIQUE.
The sequential correspondence of nucleotides in one nucleic acid molecule with those of another nucleic acid molecule. Sequence homology is an indication of the genetic relatedness of different organisms and gene function.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in plants.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
Deoxyribonucleic acid that makes up the genetic material of plants.
Periplasmic proteins that scavenge or sense diverse nutrients. In the bacterial environment they usually couple to transporters or chemotaxis receptors on the inner bacterial membrane.
Cyclic esters of acylated BUTYRIC ACID containing four carbons in the ring.
Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.
A plant species of the family SOLANACEAE, native of South America, widely cultivated for their edible, fleshy, usually red fruit.
The introduction of functional (usually cloned) GENES into cells. A variety of techniques and naturally occurring processes are used for the gene transfer such as cell hybridization, LIPOSOMES or microcell-mediated gene transfer, ELECTROPORATION, chromosome-mediated gene transfer, TRANSFECTION, and GENETIC TRANSDUCTION. Gene transfer may result in genetically transformed cells and individual organisms.
A plant genus of the family Plantaginaceae. Members contain linarin (also called acaciin).
Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which VEGETABLE PROTEINS is available.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
The process in certain BACTERIA; FUNGI; and CYANOBACTERIA converting free atmospheric NITROGEN to biologically usable forms of nitrogen, such as AMMONIA; NITRATES; and amino compounds.
Directed modification of the gene complement of a living organism by such techniques as altering the DNA, substituting genetic material by means of a virus, transplanting whole nuclei, transplanting cell hybrids, etc.
A genus of white-spored mushrooms in the family Tricholomataceae. They form symbiotic partnerships (MYCORRHIZAE) with trees.
Plants whose roots, leaves, seeds, bark, or other constituent parts possess therapeutic, tonic, purgative, curative or other pharmacologic attributes, when administered to man or animals.
A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria occurring in soil and water. Its organisms are generally nonpathogenic, but some species do cause infections of mammals, including humans.
Six-membered heterocycles containing an oxygen and a nitrogen.
Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.
Gated, ion-selective glycoproteins that traverse membranes. The stimulus for ION CHANNEL GATING can be due to a variety of stimuli such as LIGANDS, a TRANSMEMBRANE POTENTIAL DIFFERENCE, mechanical deformation or through INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS.
Any DNA sequence capable of independent replication or a molecule that possesses a REPLICATION ORIGIN and which is therefore potentially capable of being replicated in a suitable cell. (Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Enzymes that catalyze the formation of a carbon-carbon double bond by the elimination of AMMONIA. EC 4.3.1.
The relationships of groups of organisms as reflected by their genetic makeup.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
Process of generating a genetic MUTATION. It may occur spontaneously or be induced by MUTAGENS.
The presence of bacteria, viruses, and fungi in the soil. This term is not restricted to pathogenic organisms.
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
A species of gram-negative, aerobic bacteria that is found in soil and which causes formation of root nodules on some, but not all, types of field pea, lentil, kidney bean, and clover.
The genetic complement of a BACTERIA as represented in its DNA.
The relationship between two different species of organisms that are interdependent; each gains benefits from the other or a relationship between different species where both of the organisms in question benefit from the presence of the other.
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
A plant species of the family APIACEAE that is widely cultivated for the edible yellow-orange root. The plant has finely divided leaves and flat clusters of small white flowers.
A plant genus of the family MALVACEAE. It is the source of COTTON FIBER; COTTONSEED OIL, which is used for cooking, and GOSSYPOL. The economically important cotton crop is a major user of agricultural PESTICIDES.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
Benzoate derivatives substituted by one or more hydroxy groups in any position on the benzene ring.
Acetic acid derivatives of the heterocyclic compound indole. (Merck Index, 11th ed)
A plant species of the family FABACEAE widely cultivated for ANIMAL FEED.
A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
A sequence of successive nucleotide triplets that are read as CODONS specifying AMINO ACIDS and begin with an INITIATOR CODON and end with a stop codon (CODON, TERMINATOR).
A plant species of the family POACEAE. It is a tall grass grown for its EDIBLE GRAIN, corn, used as food and animal FODDER.
A plant species of the genus SOLANUM, family SOLANACEAE. The starchy roots are used as food. SOLANINE is found in green parts.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Derivatives of adipic acid. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain a 1,6-carboxy terminated aliphatic structure.
Filamentous or elongated proteinaceous structures which extend from the cell surface in gram-negative bacteria that contain certain types of conjugative plasmid. These pili are the organs associated with genetic transfer and have essential roles in conjugation. Normally, only one or a few pili occur on a given donor cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed, p675) This preferred use of "pili" refers to the sexual appendage, to be distinguished from bacterial fimbriae (FIMBRIAE, BACTERIAL), also known as common pili, which are usually concerned with adhesion.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
A cell wall-degrading enzyme found in microorganisms and higher plants. It catalyzes the random hydrolysis of 1,4-alpha-D-galactosiduronic linkages in pectate and other galacturonans. EC 3.2.1.15.

Performance and long-term stability of the barley hordothionin gene in multiple transgenic apple lines. (1/102)

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Gene knockdown by ihpRNA-triggering in the ectomycorrhizal basidiomycete fungus Laccaria bicolor. (2/102)

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Efficient Agrobacterium-based transient expression system for the production of biopharmaceuticals in plants. (3/102)

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Agrobacterium-mediated transformation of kabocha squash (Cucurbita moschata Duch) induced by wounding with aluminum borate whiskers. (4/102)

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Transformed hairy roots of Discaria trinervis: a valuable tool for studying actinorhizal symbiosis in the context of intercellular infection. (5/102)

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Characterization and crop production efficiency of diazotrophic bacterial isolates from coastal saline soils. (6/102)

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A mobile signal transported over a long distance induces systemic transcriptional gene silencing in a grafted partner. (7/102)

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FVE, an Arabidopsis homologue of the retinoblastoma-associated protein that regulates flowering time and cold response, binds to chromatin as a large multiprotein complex. (8/102)

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TY - JOUR. T1 - Agrobacterium rhizogenes-mediated transformation. T2 - Root cultures as a source of alkaloids. AU - Sevon, Nina. AU - Oksman-Caldentey, Kirsi-Marja. PY - 2002. Y1 - 2002. N2 - Hairy roots, transformed with Agrobacterium rhizogenes, have been found to be suitable for the production of secondary metabolites because of their stable and high productivity in hormone-free culture conditions. A number of plant species including many medicinal plants have been successfully transformed with Agrobacterium rhizogenes. Transformed root cultures have also been found to be a potential source of high-value pharmaceuticals. In this article the most important alkaloids produced by hairy roots are summarised. Several different methods have been used to increase the alkaloid accumulation in hairy root cultures. The selection of high productive root lines based on somaclonal variation offers an interesting option to enhance the productivity. Elicitors and modification of culture conditions have been ...
Poplar is a model organism for high in vitro regeneration in woody plants. We have chosen a hybrid poplar Populus davidiana Dode × Populus bollena Lauche. By optimizing the Murashige and Skoog medium with (0.3 mg/L) 6-benzylaminopurine and (0.08 mg/L) naphthaleneacetic acid, we have achieved the highest frequency (90%) for shoot regeneration from poplar leaves. It was also important to improve the transformation efficiency of poplar for genetic breeding and other applications. In this study, we found a significant improvement of the transformation frequency by controlling the leaf age. Transformation efficiency was enhanced by optimizing the Agrobacterium concentration (OD600 = 0.8-1.0) and an infection time (20-30 min). According to transmission electron microscopy observations, there were more Agrobacterium invasions in the 30-day-old leaf explants than in 60-day-old and 90-day-old explants. Using the green fluorescent protein (GFP) marker, the expression of MD-GFP fusion proteins in the leaf, shoot,
Background Transient gene expression via Agrobacterium-mediated DNA transfer offers a simple and fast method to analyze transgene functions. Although Arabidopsis is the most-studied model plant with powerful genetic and genomic resources, achieving highly efficient and consistent transient expression for gene function analysis in Arabidopsis remains challenging. Results We developed a highly efficient and robust Agrobacterium-mediated transient expression system, named AGROBEST (Agrobacterium-mediated enhanced seedling transformation), which achieves versatile analysis of diverse gene functions in intact Arabidopsis seedlings. Using β-glucuronidase (GUS) as a reporter for Agrobacterium-mediated transformation assay, we show that the use of a specific disarmed Agrobacterium strain with vir gene pre-induction resulted in homogenous GUS staining in cotyledons of young Arabidopsis seedlings. Optimization with AB salts in plant culture medium buffered with acidic pH 5.5 during Agrobacterium infection
Agrobacterium rhizogenes-mediated transformation is widely used in different species with various purposes. The development of composite plants (wild-type shoot with transgenic roots) has been a milestone for functional characterization of genes. Previously, composite plants were generated by two steps from inducing of hairy roots to growing in the growth medium. Hairy roots were induced in an induction medium and the growth of composite plants generated were in another different growth medium. The composite plants produced was subject to transplanting. Here, we describe an improved and optimized protocol for generation of composite plant achieved by one-step in cucumber, which has not been reported previously in living plants. Incubation of explants post inoculation to induce transgenic roots and the growth of rooted explants were in the same medium. The primary root of 5-day-old seedling was excised and the slant cut of residual hypocotyl with 1 cm length was inoculated with A. rhizogenes ...
TY - JOUR. T1 - Hairy-root organ cultures for the production of human acetylcholinesterase. AU - Woods, Ryan R.. AU - Geyer, Brian C.. AU - Leket-Mor, Tsafrir. PY - 2008/12/23. Y1 - 2008/12/23. N2 - Background: Human cholinesterases can be used as a bioscavenger of organophosphate toxins used as pesticides and chemical warfare nerve agents. The practicality of this approach depends on the availability of the human enzymes, but because of inherent supply and regulatory constraints, a suitable production system is yet to be identified. Results: As a promising alternative, we report the creation of hairy root organ cultures derived via Agrobacterium rhizogenes-mediated transformation from human acetylcholinesterase-expressing transgenic Nicotiana benthamiana plants. Acetylcholinesterase-expressing hairy root cultures had a slower growth rate, reached to the stationary phase faster and grew to lower maximal densities as compared to wild type control cultures. Acetylcholinesterase accumulated to ...
See on Scoop.it - Virology and Bioinformatics from Virology.caThe flagellotropic phage 7-7-1 infects motile cells of Agrobacterium sp H13-3 by attaching to and traveling along the rotating flagellar filament to the secondary receptor at the base, where it injects its DNA into the host cell. This is an interesting paper, because it describes a…
Sorghum is the fifth most widely planted cereal crop in the world and is commonly cultivated in arid and semi-arid regions such as Africa. Despite its importance as a food source, sorghum genetic improvement through transgenic approaches has been limited because of an inefficient transformation system. Here we report a ternary vector (also known as co-habitating vector) system using a recently described pVIR accessory plasmid that facilitates efficient Agrobacterium-mediated transformation of sorghum. We report regeneration frequencies ranging from 6-29% in Tx430 using different selectable markers and single copy, backbone free quality events ranging from 45-66% of the total events produced ...
We have established an efficient Agrobacterium-mediated transformation procedure for Arabidopsis thaliana genotype C24 using the chimeric bialaphos resistance gene (bar) coding for phosphinothricin ac
The exploration of space is one of the most inspiring areas of scientific research and a major driver of technological innovation. One of the major factors…
There are a lot of good conferences coming up this year, some small and some very big; some free and some rather more expensive. If you are put off by registration or accommodation fees, you dont have to be. There are grants available for people to travel to conferences and workshops, and Ive listed some below. I havent mentioned grants for specific conferences, but most learned societies offer grants for students and new post-docs to attend their events. Please let me know in the comments or via email (charis @ garnetcommunity.org.uk) or Twitter if you know of any general funds which I havent listed.. Not that Im biased (of course…) but the GARNet workshop on Plant Synthetic Biology and UK PlantSci 2013 are excellent opportunities to use these funds! Plant Synthetic Biology registration starts from £175 for students, including accommodation, lunches, and conference dinner. Early bird registration for PlantSci 2013 starts from £80 for students, including lunch and refreshments, but you ...
We have identified quantitative trait loci (QTL) for transgenic and adventitious root production using an Agrobacterium rhizogenes-mediated co-transformati
Transgenic tomato plants of south Indian cultivar Arka Vikas were developed using Agrobacterium strain EHA 105, harbouring Bt Cry2A gene with a construct containing 35S CaMV promoter, OCS terminator and nptII selectable marker, through Agrobacterium-mediated transformation. This study was conducted to improve the regeneration and transformation protocol for south Indian cultivar Arka Vikas. Hypocotyl was used as explant source for transformation due to high regeneration efficiency, molecular analysis through PCR for putative transformants in T0 generation and qualitative ELISA method was performed for Bt protein expression followed by insect bioassays. Insect bioassay studies was conducted using neonate larva of helicoverpa armigera to screen the plants and the plants expressing good resistance with molecular and phenotypic characters were carried further for successive generations. The experimental results concluded that Bt gene was deployed in tomato cultivar successfully and had developed resistance
The Harvard iGarden is a venture into plant engineering. Our aim is to create a toolkit for the cultivation of a personalized garden containing features introduced through synthetic biology. We are currently working on two independent features to be included in this toolkit - inclusion of novel flavors and knockdown of plant allergens. In addition, we are working on the creation of a genetic fence to prevent the spread of foreign genetic material. All components are designed to fit the biobrick standard.. We are introducing biobrick parts to plants through agrobacterium-mediated transformation. To do so, we have modified existing agrobacterium vectors designed for plant transformation to include the biobrick multiple cloning site.. The Harvard iGarden, beyond being a simple application of the biobrick system to plant engineering, is an effort to raise public awareness of synthetic biology by bringing its effects down to a personal and more tangible level. We envision the iGarden as an ...
OpenPlant: sharing tools for a sustainable future. OpenPlant is a joint initiative in plant synthetic biology between the University of Cambridge, John Innes Centre and the Earlham Institute, funded by the BBSRC and EPSRC as part of the UK Synthetic Biology for Growth programme, directed in Cambridge by Prof. Jim Haseloff and in Norwich by Prof. Anne Osbourn.
OpenPlant: sharing tools for a sustainable future. OpenPlant is a joint initiative in plant synthetic biology between the University of Cambridge, John Innes Centre and the Earlham Institute, funded by the BBSRC and EPSRC as part of the UK Synthetic Biology for Growth programme, directed in Cambridge by Prof. Jim Haseloff and in Norwich by Prof. Anne Osbourn.
A World Leader in Plant Synthetic Biology: Understanding Plants and Using Our Plant Knowledge for Human and Environmental Use ...
Standardized DNA assembly strategies facilitate the generation of multigene constructs from collections of building blocks in plant synthetic biology. A common syntax for hierarchical DNA assembly following the Golden Gate principle employing Type IIs restriction endonucleases was recently developed, and underlies the Modular Cloning and GoldenBraid systems. In these systems, transcriptional units and/or multigene constructs are assembled from libraries of standardized building blocks, also referred to as phytobricks, in several hierarchical levels and by iterative Golden Gate reactions. This combinatorial assembly strategy meets the increasingly complex demands in biotechnology and bioengineering, and also represents a cost-efficient and versatile alternative to previous molecular cloning techniques. For Modular Cloning, a collection of commonly used Plant Parts was previously released together with the Modular Cloning toolkit itself, which largely facilitated the adoption of this cloning ...
Bhatia, R, Dalton, SJ, Roberts, LA, Gallagher, JA & Bosch, M 2014, Genetic engineering of lignocellulosic biomass in grasses: from gene function analysis to targeted in planta deconstruction, First International Workshop on Plant Synthetic Biology, Boston, United States of America, 17 May 2014 - 18 May 2014 ...
Plant scientists are now using genome editing tools to explore on gene function and develop crops for improvement of traits. One of the technical challenges in using such tools is to efficiently induce precise and predictable targeted point mutations for crop breeding. Thus, new additional tools have been developed such as cytidine base editors (CBEs), which are CRISPR-Cas9 derived tools used to direct cytidine to thymine base conversion. In dicots, the most stable genomic integration of CRISPR-Cas9 is through Agrobacterium-mediated transformation. However, elimination of the foreign DNA may be hard to accomplish, particularly in vegetatively propagated plants ...
Agrobacterium-mediated transformation: Agrobacterium-mediated transformation has been used to create commercial cultivars for over 10 years and is known to create insertion-site mutations (Table 2, Section 1.1). However, there has been only one large-scale study of the mutations created at insertion events2 containing single T-DNA3inserts (the type of event preferred for commercial purposes; Forsbach et al. 2003). In this study of 112 single-copy T-DNA insertion events in A. thaliana, the researchers found that exact T-DNA integration almost never occurred (Forsbach et al. 2003). Most of the T-DNA insertions resulted in small (1-100 base pair) deletions of plant genomic sequences at the insertion-site. However, for a significant number (24/112) there was evidence for large-scale rearrangement of plant genomic DNA at the insertion-site. Two of these insertion events contained chromosomal translocations. The rest had rearrangements which were not fully characterised. It is known, however, that ...
pCAMBIA3301 Informaiton Promoter: Lac, CaMV 35S Replicon: pVS1 oriV, ori Terminator: NOS Plasmid classification: plant series, protein overexpression vector Plasmid size: 11319bp Prokaryotic resistance: Kan Selection marker: Bar Clonal strain: DH5 alpha Culture conditions: 37 C, aerobic LB Expression host: plant cells 5sequencing primers: M13F:TGTAAAACGACGGCCAGT Primers for 3sequencing: primers designed based on sequences Use: Plant expression
5CASD3.xxxx-00 SDL3/SDL4 cables are designed to transfer SDL3/SDL4 data and enable easy cable installation. Due to the RJ45 connector, the cable is also suitable for narrow feed-throughs, e.g. in swing arm shafts.. ...
Profiles of Sina (upper line) and Sinb (lower line) gene expression in developing spikes of the non-transgenic plants of cv. Wanad from 8th to 32nd day after po
The following protocol is designed for NEB 10-beta Competent E. coli (NEB #C3019 ) which are included in the NEB PCR Cloning Kit (NEB #E1202 ) only
There is a broad range of methods that are employed in the process of producing GMOs. This often involves insertion of a gene of interest into living organisms depending on the species that you are working on. In plants mainly, two most common biotechnology-based techniques include; Agrobacterium-mediated transformation and bombardment of particles. According to the regulations given by FSANZ, it is a requirement that clear description of the method employed in genetically engineering plants is given.. Case study- Roundup Ready soy. This was produced using the particle bombardment method. This process of biotechnologically engineering soybeans involved; bombardment of the plant cells with microscopic particles of gold coated with DNA that contains the gene of interest. The gene of interest is the EPSPS gene that is derived from Agrobacterium. The aim of this is to introduce the novel gene of interest through the cell wall so that it integrates into the genetic material of the soy plant. The new ...
USING YEAST AS MODEL, A SCREENING WAS PERFORMED AND MON2 WAS IDENTIFIED AS A NOVEL HOST FACTOR THAT WAS INVOLVED IN AGROBACTERIUM-MEDIATED TRANSFORMATION (AMT) OF YEAST. FURTHER STUDIES HAVE REVEALED THAT MON2 MEDIATES TRANSIENT AMT NEGATIVELY. WHILE AT THE SAME TIME PLAY A POSITIVE ROLE IN MEDIATING STABLE AMT ...
On April 12, CASHS students in the autism support program hosted their younger counterparts from Hamilton Heights Elementary School: Cooking and serving lunch to them, entertaining them at a choral concert, reading to them and helping them put their hand prints on an Autism Awareness banner.
SEQUENCE 797 AA; 91680 MW; 28EA7CA7A1DE223C CRC64; MAALAYNLGK REINHYFSVR SAKVLALVAV LLLAACHLAS RRYRGNDSCE. YLLSSGRFLG EKVWQPHSCM MHKYKISEAK NCLVDKHIAF IGDSRIRQLF. YSFVKIINPQ FKEEGNKHEN IPFEDKTASV KVDFLWHPEV NGSMKQCIKV. WTEDSIAKPH VIVAGAATWS IKIHNGSSEA LSQYKMNITS IAPLLEKLAK. TSDVYWVLQD PVYEDLLSEN RKMITNEKID AYNEAAVSIL NSSTRNSKSN. VKMFSVSKLI AQETIMESLD GLHLPESSRE TTAMILMNVY CNKILKPVDG SCCQPRPPVT LIQKLAACFF TLSIIGYLIF YIIHRNAHRK NKPCTDLESG. EEKKNIINTP VSSLEILLQS FCKLGLIMAY FYMCDRANLF MKENKFYTHS. SFFIPIIYIL VLGVFYNENT KETKVLNREQ TDEWKGWMQL VILIYHISGA. STFLPVYMHI RVLVAAYLFQ TGYGHFSYFW IKGDFGIYRV CQVLFRLNFL. VVVLCIVMDR PYQFYYFVPL VTVWFMVIYV TLALWPQIIQ KKANGNCFWH. FGLLLKLGFL LLFICFLAYS QGAFEKIFSL WPLSKCFELK GNVYEWWFRW RLDRYVVFHG MLFAFIYLAL QKRQILSEGK GEPLFSNKIS NFLLFISVVS. FLTYSIWASS CKNKAECNEL HPSVSVVQIL AFILIRNIPG YARSVYSSFF. AWFGKISLEL FICQYHIWLA ADTRGILVLI PGNPMLNIIV STFIFVCVAH. EISQITNDLA QIIIPKDNSS LLKRLACIAA FFCGLLILSS IQDKSKH ...
reproduction: revisions to the UC Davis Powdery Mildew Risk Index. PLANT DISEASE 97(11): 1438-1447. ... F, Marchi, G, Surico, G. (2010). Indexing Agrobacterium vitis in asymptomatic grapevine .... ...
reproduction: revisions to the UC Davis Powdery Mildew Risk Index. PLANT DISEASE 97(11): 1438-1447. ... F, Marchi, G, Surico, G. (2010). Indexing Agrobacterium vitis in asymptomatic grapevine .... ...
Za vse karantenske kodljive organizme bo tudi v prihodnje potrebno v Sloveniji zagotoviti temeljit nadzor, za karantenske kodljive organizme s seznama A1, ki so bili v Sloveniji odkriti v zadnjem asu, pa se bo treba opredeliti tudi do njihovega prihodnjega statusa. Tudi na splo no bo potrebno status karantenskih kodljivih organizmov na slovenskih karantenskih listah ponovno preveriti in jih uskladiti z Aneksom I Smernice 77/93/EC.. Portoro , 4. marca 1999. Poro ilo pripravila predsedujo a sekcije Jo i Jerman Cvelbar. Sekcija »Varstvo vinske trte«. Sekcija je obravnavala aktualno problematiko in novej a spoznanja na podro ju varstva vinske trte, diagnostike nekaterih problemati nih bolezni, zlasti raka vinske trte. S stali a prispevka k znanosti je potrebno posebej izpostaviti referata S. Vrabla o okolju prijazni metodi zbeganja pri zatiranju grozdnih suka ev ter M. abec o raku vinske trte (Agrobacterium vitis). Portoro , 4. marca 1999. Poro ilo pripravil predsedujo i sekcije dr. Stojan ...
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Synthetic biology is producing a paradigm shift in biotechnology based on the introduction of engineering principles in the design of new organisms by genetic modification (Check, 2005; Haseloff and Ajioka, 2009). Whereas synthetic biology has rapidly permeated microbial biotechnology, the engineering of multicelled organisms following synthetic biology principles is now emerging and is mainly driven by the so-called top-down approaches, where newly engineered genetic circuits are embedded into naturally existing organisms used as a chassis. The plant chassis offers an extraordinarily fertile ground for synthetic biology-like engineering. However, technology still faces the huge challenge of performing engineering-driven genetic designs. One of the main technological challenges of plant synthetic biology requires the construction and transfer of multigene structures to the plant genome. This is putting pressure on developing DNA assembly and transformation technologies adapted to plants. One ...
Recent studies on the root proteome of Medicago truncatula (Gaertn.) showed an induction of pathogenesis-related (PR) proteins of the class 10 after infection with the oomycete pathogen Aphanomyces euteiches (Drechs.). To get insights into the function of these proteins during the parasitic root-microbe association, a gene knockdown approach using RNAi was carried out. Agrobacterium rhizogenes-mediated transformation of M. truncatula roots led to a knockdown of the Medicago PR10-1 gene in transgenic in vitro root cultures. Proteomic analyses of the MtPr10-1i root cultures showed that MtPr10-1 was efficiently knocked down in two MtPr10-1i lines. Moreover, five additional PR-10-type proteins annotated as abscisic acid responsive proteins (ABR17s) revealed also an almost complete silencing in these two lines. Inoculation of the root cultures with the oomycete root pathogen A. euteiches resulted in a clearly reduced colonization and thus in a suppressed infection development in MtPr10-1i roots as ...
Ice plant (Mesembryanthemum crystallinum L.) is a model plant for studying salt-tolerant mechanisms in higher plants. Many salt stress-responsive ice plant genes have been identified with molecular and biochemical approaches. However, no further functional characterization of these genes in host plant due to lack of easy and effective transformation protocols. To establish efficient transformation system of ice plants, three types of ice plant materials, hypocotyl-derived callus, aseptically-grown seedlings and pot-grown juvenile plants, were used to develop Agrobacterium-mediated transformation protocols. The highest transient transformation efficiency was with 5-day-old ice plant callus co-incubated with an Agrobacterium tumefaciens at 2.5 × 109 cells mL−1 for 48 h. The 3-day-old ice plant seedlings with root tip removed were successfully infected with A. tumefaciens or A. rhizogenes, and obtained 85% and 33-100% transient transformation rates, respectively. The transient transformation assays in
Sarker, R.H. and Biswas, A. (2002) In Vitro Plantlet Regeneration and Agrobacterium-Mediated Genetic Transformation of Wheat (Triticum aestivum L.) Plant Tissue Culture, 12, 155-165.
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Read Sonication-assisted Agrobacterium-mediated transformation of the ACC gene to interfere the production of ethylene in spring Dendrobium cv.
Transformation Protocol. BY-2 (NT1) Cell Transformation with Agrobactrium. Day 1: 1. Grow up 1 ml of the Agrobacterium overnight in LB + all selective drugs. This culture may be started from frozen glycerol cultures if necessary. Day 2: 2. NT cells are used 3 days after splitting the NT cell culture. 4 ml of NT cells are required for each transformation with an additional 4 ml for the control culture which receives no bacteria. 3. 1 ul Acetosyringone (20 mM in ethanol) is added per ml of NT cells. Typically treat the whole 50 ml culture at this point and discard any that is left over when Im finished. 4. Using a 10 ml pipette, the NT cells are pipetted in and out about 20 times. This helps to induce small lesions in the cells and increases the efficiency of the transformation. 5. 75 ul of bacteria (dense growth) or 100 ul (moderate growth) are added to a petri dish containing 4 ml of NT cells (from step 4) and mixed thoroughly. REMEMBER TO INCLUDE A CONTROL HAVING NO BACTERIA. 6. Wrap plates ...
Date Published: July 6, 2015. Publisher: Public Library of Science. Author(s): Anna Coll, Mandy L. Wilson, Kristina Gruden, Jean Peccoud, Martina Stromvik.. http://doi.org/10.1371/journal.pone.0132502. Abstract. Plant synthetic biology requires software tools to assist on the design of complex multi-genic expression plasmids. Here a vector design strategy to express genes in plants is formalized and implemented as a grammar in GenoCAD, a Computer-Aided Design software for synthetic biology. It includes a library of plant biological parts organized in structural categories and a set of rules describing how to assemble these parts into large constructs. Rules developed here are organized and divided into three main subsections according to the aim of the final construct: protein localization studies, promoter analysis and protein-protein interaction experiments. The GenoCAD plant grammar guides the user through the design while allowing users to customize vectors according to their needs. ...
Mungbean is an important pulse crop extensively cultivated in Southeast Asia for supply of easily digestible protein. Salinity severely limits the growth and productivity of mungbean, and weeding poses nutritional and disease constraints to mungbean cultivation. To pyramid both salt tolerance and protection against herbicide in mungbean, the AtNHX1 encoding tonoplast Na+/H+ antiporter from Arabidopsis, and bar gene associated with herbicide resistance were co-expressed through Agrobacterium-mediated transformation. Stress inducible expression of AtNHX1 significantly improved tolerance under salt stress to ionic, osmotic and oxidative stresses in transgenic mungbean plants compared to the wild type (WT) plants, whereas constitutive expression of bar provided resistance to herbicide. Compared to WT, transgenic mungbean plants grew better with higher plant height, foliage, dry mass and seed yield under high salt stress (200 mM NaCl) in the greenhouse. The improved performance of transgenic plants under
The study was carried out to evaluate the amenability of tropical inbred and hybrid maize lines, using Agrobacterium mediated transformation technique. Agrobacteriumtumefaciens strains EHA101 harbouri
The developments in transformation technology have enabled the scientists to incorporate, mutate or substitute gene(s) leading to a particular trait; advancing it to a point where only few technical limitations remain. Genotype...
The diguanylate cyclase from Agrobacterium vitis has been engineered to remove phosphodiesterase activity, allowing for production of cyclic-diGMP from guanosine triphosp
In an effort to engineer plants to be resistant to glyphosate, scientists starting looking for glyphosate resistant EPSP in the 1980s. Several methods were employed including selection, directed evolution, site-directed mutagenesis, and microbial screens. It was difficult to obtain a suitable enzyme because typically the resistant enzymes had an undesirable decrease in catalytic activity. Eventually, naturally occurring glyphosate-tolerant microbes were identified including Agrobacterium sp. Strain CP4, Achromobacter sp. Strain LBAA, and Pseudomonas sp. Strain PG2982. The enzymes isolate from these maintained good catalyctic ativity while being resistant to glyphosate (Funke). Agrobacterium sp. Strain CP4 was obtained from a glyphosate rich waste area at a glyphosate production facility. There is substantial sequence variation between these resistant enzymes and those of plants or E. coli. Other versions resistant versions of EPSP have been identified in Streptococcus pneumonia and ...
Bar gene coding Glufosinate resistance is transformed into the alfalfa variety, Caoyuan No.1, mediated by Agrobacterium tumefaciens. The leaves of transgenic plants after being screened and tested are proved to be resistant to Glufosinate. Furthermore, PCR analysis shows that bar gene has been transformed into Alfalfa.
The aim of the proposed project (in collaboration with Geneticlab S.r.l.) is to design reliable protocols to produce biomass and to induce lipid accumulation in cell cultures obtained from a plant species known as high oleic: Jatropha curcas. This species has attracted the interest of various developmental agencies in the tropics and subtropics due to its easy adaptability to semi-arid marginal sites, use of the oil as a diesel fuel substitute and its use in erosion control. J. curcas is monoecious, with male and female flowers on the same plant, and its center of origin are Mexico and Central America. In its natural area of distribution, the species is most abundant in tropical savanna and monsoon climates and in temperate climates without a dry season and with a hot summer ...
Hello researchers, , ,I am study one gene promoter and fused it to GUS in pCAMBIA1391Z, in which ,CaMV35S drives the selectable marker Hyg gene. Among the 10 independent T3 ,lines, which were confirmed by PCR, some show very strong GUS staining, ,some no, and some weak staining. , ,I knew from this paper that vectors containing 35S promoter driving ,selectable marker gene are not good for promoter study. ,The 35S promoter used in a selectable marker gene of a plant transformation ,vector affects the expression of the transgene. Planta. Volume 221, Number ,4 / June, 2005 , ,But I found that quite many published papers used vectors (like ,pCAMBIA1391, pCAMBIA1301, etc) containing 35S promoter driving selectable ,marker. , ,As the staining patterns of my promoter fusion lines are not consistent at ,al, I want to use another vector pBI121 or pCGN1547. Does anyone know where ,to get pCGN1547? ,How do you deal with those lines from 35S containing vector? CAMBIA ,suggests doing co-transformation ...
Bifunctional protein GlmU; Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP- GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5- monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal ...
Protein translocase subunit SecY; The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move ...
We will carry out an empirical study on how mobility dynamics affect emigration from France. We will do so by widening the study to other population categories than simply immigrants (second generation, and expatriates). ...
is provided, focused on the role played by the different components of the virulence system. The general assessments for the establishment of efficient transformation protocols are discussed with an emphasis in the application of this methodology to monocotyledonous plants. Based on our own experience, we present the establishment of sugarcane transformation by A. tumefaciens as a model of application of this methodology to an important culture plant species, previously considered recalcitrant and inaccessible for this type of genetic manipulation ...
Agrobacterium-medieret transformation ved hjælp af en blomster-dip metode kan anvendes med succes at skabe stabile transgene linjer af...
Triplex End-Point PCR kit. The major symptoms of infection by Agrobacterium vitis are galls found on the lower trunk, near the soil line, but galls can also develop slightly below the soil surface, or extend up to 1-m height. Infected plants produce inferior shoots, and portions of the vine above the gall may die. A. vitis mainly enters the plant through wounds caused by freezing, so climatic conditions favouring freeze injury also favour infection by A. vitis. Contaminated planting material can, however, also be a source of inoculum.. Source: EPPO Bulletin 32, 367-369. ...
Agrobacterium vitis genes for 16S rRNA, tRNA-Ile, tRNA-Ala, 23S rRNA 150-b fragment, IVS, 23S rRNA 1.3-kb fragment A, partial and complete sequence, strain: NCPPB-1771, locus: ...
A enzima mio-inositol-1-fosfato sintase (MIPS1) (E.C.5.5.1.4) catalisa a conversão irreversível de D-Glicose-6-P para 1-L-mio-inositol-1-P. O mio-inositol desempenha papel de destaque no metabolismo vegetal, fornecendo inositol e inositídeos em processos metabólicos essenciais à formação do vegetal. Dentre as várias vias nas quais o mio-inositol está envolvido algumas são alvo do melhoramento vegetal, a exemplo a manipulação do conteúdo de ácido fítico e de oligossacarídeos em sementes e açúcares estruturais na parede celular. Até o presente momento, plantas MIPS silenciadas não foram caracterizadas em detalhes, principalmente em suas estruturas vegetativas. Com isso, o presente trabalho objetivou caracterizar tomateiros Moneymaker com diferentes níveis de silenciamento de MIPS1. Para a indução do silenciamento utilizou- se tranformação genética via Agrobacterium tumefaciens contendo plasmídeo pCambia com construção tipo intron hairpin de fragmentos do gene MIPS de ...
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Supplementary MaterialsSupplementary Shape 1. and viability assay had been performed. Outcomes: With this research, we display that BM-MSCs can induce the EMT development of CRC cells Delpazolid test, CRC shown the morphological features of epithelialCmesenchymal changeover after co-cultured with BM-MSCs for 72?h (Supplementary Shape 1A). To recognize whether MSC-CRC cell-cell adhesion was very important to this alteration further, three different co-culture versions had been founded. After Delpazolid 72?h co-cultivation in ibidi 31.9%, 11.730.9979, CRC+MSC, 603.8 MSC, 297) in cancer cells from co-cultivation organizations. Cancers cells underwent epithelial-mesenchymal changeover and MSC differentiated into adult cancer-associated fibroblasts (CAF) in the co-culture model In the MSC-CRC wound-healing assay, MSCs demonstrated greater flexibility than CRC cells (Supplementary Shape 1B). Besides, MSCs exhibited some morphological adjustments, including elongated phenotype, decreased adhesion, and ...
Co-cultivation of production cells at the limit of the in vitro age is an assay we offer to test for latent retroviruses for our clients. Essentially, your production cells are plated along with susceptible cell lines that exhibit stronger phenotypic CPE when the virus enters them. Thus, viruses that are expressed as non-infectious particles or exhibit low level of expression in production cells are detected ...
Co-cultivation of production cells at the limit of the in vitro age is an assay we offer to test for latent retroviruses for our clients. Essentially, your production cells are plated along with susceptible cell lines that exhibit stronger phenotypic CPE when the virus enters them. Thus, viruses that are expressed as non-infectious particles or exhibit low level of expression in production cells are detected ...
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一基本信息姓名:周虚出生年月:1965年8月15日生职称:吉林大学动物科学学院教授、博导联系方式:0431-87835142,Email:[email protected]招生学科:动物遗传育种与繁殖研究方向:动物生殖调控二研究特色1.牛、猪卵泡和早期胚胎发育调控的分子机制2.营养对母猪繁殖的影响及其内分泌和分子机制3.猪繁殖障碍检测与防治三学术任职教育部高校教学指导委员会委员中国奶业协会繁殖专业委员会副主任中国畜牧兽医学会动物繁殖分会常务理事、副秘书长吉林省畜牧兽医学会常务理事四学历和学位1981
Main article: Agrobacterium. Based on molecular data it was shown that the genus Agrobacterium is nested in Rhizobium and the ... Given the plant pathogenic nature of Agrobacterium species, it was proposed to maintain the genus Agrobacterium[95] and the ... Farrand, S.; Van Berkum, P.; Oger, P. (2003). "Agrobacterium is a definable genus of the family Rhizobiaceae". International ... Young, J.; Kuykendall, L.; Martínez-Romero, E.; Kerr, A.; Sawada, H. (2003). "Classification and nomenclature of Agrobacterium ...
Agrobacterium tumefaciens Pierce's disease Xylella fastidiosa Bacterial inflorescence rot [1] Pseudomonas syringae ...
Agrobacterium'i liigid on taimedega seotud Rhizobia sugulased. Mitu Agrobactericumi liiki põhjustab taimehaigusi, nagu ... Agrobacterium-vahendatud taimede ümberkujundamine on väga keeruline protsess, mis hõlmab nii bakteri kui ka peremeestaime ... "Agrobacterium-Mediated Plant Transformation: the Biology behind the "Gene-Jockeying" Tool". 2003. American Society for ... Geenmuundatud organismide tehnoloogia aluseks on mehhanism, mida Agrobacterium kasutab oma T-DNA sisaldamiseks taimede ...
Agrobacterium rhizogenes insert T-DNA into the genome of the host plant root cells. nature. 1982; 295:432-4 Cardarelli M, ... Rhizobium rhizogenes (formerly Agrobacterium rhizogenes) is a Gram-negative soil bacterium that produces hairy root disease in ... "Agrobacterium rhizogenes T-DNA genes capable of inducing hairy root phenotype". Molecular and General Genetics 209(3):475-480. ... Intrieri, M. C. & Buiatti, M. (July 2001). "The horizontal transfer of Agrobacterium rhizogenes genes and the evolution of the ...
Van Keer C, Kersters K, De Ley J (September 1976). "L-Sorbose metabolism in Agrobacterium tumefaciens". Antonie van Leeuwenhoek ...
Using Agrobacterium-mediated transformation, a technique that takes advantage of the natural process by which Agrobacterium ... Genetic transformation of A. thaliana is routine, utilizing Agrobacterium tumefaciens to transfer DNA into the plant genome. ... The current protocol, termed "floral dip", involves simply dipping flowers into a solution containing Agrobacterium carrying a ... Zhang X, Henriques R, Lin SS, Niu QW, Chua NH (2006). "Agrobacterium-mediated transformation of Arabidopsis thaliana using the ...
One of these was isolated from an Agrobacterium strain CP4 (CP4 EPSPS) that was resistant to glyphosate.[123][124] The CP4 ... Agrobacterium tumefaciens-mediated transformation is another common technique. Agrobacteria are natural plant parasites.[51] ... Shrawat, A.; Lörz, H. (2006). "Agrobacterium-mediated transformation of cereals: a promising approach crossing barriers". Plant ... When Agrobacterium infects a plant, it transfers this T-DNA to a random site in the plant genome. When used in genetic ...
Agrobacterium tumefaciens..." ...
"Lipid-bond Saccharides containing glucose and galactose in agrobacterium tumefaciens", (1984). *. Zorreguieta, A.; Ugalde, R.A ...
It was discovered in Agrobacterium tumefaciens, which uses this system to introduce the T-DNA portion of the Ti plasmid into ... Christie PJ (November 2004). "Type IV secretion: the Agrobacterium VirB/D4 and related conjugation systems". Biochimica et ... The prototypic Type IVA secretion system is the VirB complex of Agrobacterium tumefaciens.[11] ...
In plants the DNA is often inserted using Agrobacterium-mediated transformation,[59] taking advantage of the Agrobacteriums T- ... Plant genomes can be engineered by physical methods or by use of Agrobacterium for the delivery of sequences hosted in T-DNA ... "Agrobacterium-mediated plant transformation: the biology behind the "gene-jockeying" tool". Microbiology and Molecular Biology ...
Such elements as the Agrobacterium Ti or Ri plasmids contain elements that can transfer to plant cells. Transferred genes enter ... It was discovered in Agrobacterium tumefaciens, which uses this system to introduce the Ti plasmid and proteins into the host, ... which develops the crown gall (tumor).[6] The VirB complex of Agrobacterium tumefaciens is the prototypic system.[7] ...
cyclosophorans (Agrobacterium spp., Rhizobium spp. and Xanthomonas spp.). *dextran (Leuconostoc mesenteroides, Leuconostoc ... galactoglucopolysaccharides (Achromobacter spp., Agrobacterium radiobacter, Pseudomonas marginalis, Rhizobium spp. and Zooglea ...
"Rhizobium/Agrobacterium group". National Center for Biotechnology Information (NCBI) taxonomy database. Retrieved 2012-05-02.. ...
... oncogenic bacteria in genus Agrobacterium.[citation needed] The virA gene on the Ti plasmid in the genome of Agrobacterium ... These compounds are typically found from wounded plants, and as a result VirA is used by Agrobacterium tumefaciens to locate ... "Sequence analysis of the vir-region from Agrobacterium tumefaciens octopine Ti plasmid pTi15955". Journal of Experimental ... "Acetosyringone promotes high efficiency transformation of Arabidopsis thaliana explants by Agrobacterium tumefaciens". Plant ...
Agrobacterium species change the level of auxins to cause tumours with phytohormones. Exopolysaccharides are produced by ...
"Lipids in hairy roots and non-Agrobacterium induced roots of Crambe abyssinica". Acta Physiologiae Plantarum. 35 (7): 2137-2145 ...
Esta inclúe Agrobacterium tumefaciens (Smith e Townsend 1907) Conn 1942. *↑ Blastobacter capsulatus está clasificada ... 9,0 9,1 9,2 Estas especies estaban anteriormente clasificadas no xénero Agrobacterium. ... xunto con parasitos de plantas como Agrobacterium. As Rhizobiaceae son gramnegativas, aerobias e xeralmente con forma de bacilo ... International Committee on Systematics of Prokaryotes Subcommittee on the taxonomy of Agrobacterium and Rhizobium. Minutes of ...
Genus Agrobacterium. *Genus Rhizobium / Sinorhizobium(synonymous). *Genus Liberibacter (candidatus). *Famili Rhodobiaceae * ...
For example, the tumor-inducing (Ti) plasmid of Agrobacterium and the root-tumor inducing (Ri) plasmid of A. rhizogenes contain ... Agrobacterium-like conjugation is also primarily used for dicots, but monocot recipients are not uncommon. ... Pan SQ, Jin S, Boulton MI, Hawes M, Gordon MP, Nester EW (July 1995). "An Agrobacterium virulence factor encoded by a Ti ... In plant engineering, Agrobacterium-like conjugation complements other standard vehicles such as tobacco mosaic virus (TMV). ...
They are growing by unipolar growth, like Agrobacterium tumefaciens, Sinorhizobium meliloti and Ochrobactrum anthropi. They ...
"Agrobacterium: the natural genetic engineer (100 Years Later)". Retrieved 14 January 2011.. CS1 maint: discouraged parameter ( ... In 1907 a bacterium that caused plant tumors, Agrobacterium tumefaciens, was discovered and in the early 1970s the tumor ...
Specific strains include Agrobacterium radiobacter ATCC 55002[31] and the sub-branches of Proteobacteria like BNC1, BNC2,[32] ... Agrobacterium radiobacter only degrades Fe(III) EDTA[32] while BNC1 and DSM 9103 are not capable of degrading iron(III) EDTA ... N lyase found in Agrobacterium tumefaciens (BY6), which can be harvested on a large scale. Additionally, the reactions ... "Biodegradation of All Stereoisomers of the EDTA Substitute Iminodisuccinate by Agrobacterium Tumefaciens BY6 Requires an ...
Kemner JM, Liang X, Nester EW (Apr 1997). "The Agrobacterium tumefaciens virulence gene chvE is part of a putative ABC-type ... The chvE-gguAB gene in Agrobacterium tumefaciens encodes glucose and galactose importers that are also associated with ... Cangelosi GA, Ankenbauer RG, Nester EW (Sep 1990). "Sugars induce the Agrobacterium virulence genes through a periplasmic ...
Schell, J.; Van Montagu, M. (1977). The Ti-plasmid of Agrobacterium tumefaciens, a Natural Vector for the Introduction of Nif ... Agrobacterium tumefaciens, a soil rhizosphere bacterium, can attach to plant cells and infect them with a callus-inducing Ti ...
Other methods exploit natural forms of gene transfer, such as the ability of Agrobacterium to transfer genetic material to ... They infected tobacco with Agrobacterium transformed with an antibiotic resistance gene and through tissue culture techniques ... allowing transformation of plants not susceptible to Agrobacterium infection.[23] In 2000, Vitamin A-enriched golden rice, was ...
Agrobacterium,[11] a plant pathogen (oncogenic) genus of Gram-negative bacteria, also of biotechnologic use ... "Carbohydrate catabolism of selected strains in the genus Agrobacterium". Appl Microbiol. 30 (5): 731-7. doi:10.1128/AEM.30.5. ...
... a través da substitución de Agrobacterium sp. (cepa CP4) xene síntese do EPSP. A version substituída non é sensible ao ...
Agrobacterium-mediated T-DNA transfer is widely used as a tool in biotechnology. For more than two decades, Agrobacterium ... To interact with host plant proteins many Agrobacterium virulence proteins encoded by vir genes. Agrobacterium vir genes ... plasmid of some species of bacteria such as Agrobacterium tumefaciens and Agrobacterium rhizogenes. The T-DNA is transferred ... thus forming the crown gall tumors typically induced by Agrobacterium tumefaciens infection. Whereas Agrobacterium rhizogenes ...
Šādu scenāriju izmanto Pseudomonas savastanoi un Agrobacterium tumefaciens.[2] IAA sintēze bez triptofāna[labot šo sadaļu , ...
Agrobacterium tumefaciens, Agrobacterium rhizogenes, and Agrobacterium vitis. Strains within Agrobacterium tumefaciens and ... Agrobacterium nepotum (Puławska et al. 2012) Mousavi et al. 2015. *Agrobacterium pusense (Panday et al. 2011) Mousavi et al. ... Agrobacterium rubi (Hildebrand 1940) Starr and Weiss 1943. *Agrobacterium skierniewicense (Puławska et al. 2012) Mousavi et al ... Agrobacterium tumefaciens is the most commonly studied species in this genus. Agrobacterium is well known for its ability to ...
Brief Description of Agrobacterium tumefaciens C58:. A. tumefaciens C58 is the most heavily studied member of the genus ... Scientists know a lot now about virulent Agrobacterium strains do this and have worked out methods to use this natural DNA ... However, most people know Agrobacterium because some strains can do something that no other cellular pathogen does - inject a ... Agrobacterium. Members of this genus are commonly found in soils all over the world as a aerobic heterotroph that can use a ...
... Steve Wylie wylie at CENTRAL.MURDOCH.EDU.AU Tue Oct 10 21:20:46 EST 1995 *Previous message: Geneclean ... Does anyone else working with Agrobacterium tumefaciens have the problems that I do? Firstly, it is very inconsistant in growth ...
Now I want the blank cultures of Agrobacterium , strains, LBA 4404, EHA 105 and EHA 101 so that I can introduce my vector , ... Agrobacterium strains. Dr Engelbert Buxbaum engelbert_buxbaum at hotmail.com Thu Jun 8 07:13:43 EST 2006 *Previous message: How ... Can you please , tell me how to get these Agrobacterium strains. There are microbiological type culture collection, for example ...
Agrobacterium tumefaciens (updated scientific name Rhizobium radiobacter, synonym Agrobacterium radiobacter) is the causal ... Agrobacterium tumefaciens overwinters in infested soils. Agrobacterium species live predominantly saprophytic lifestyles, so ... "Rhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium radiobacter)". UniProt Taxonomy. Archived from the original on ... Agrobacterium performs this control by inserting the plasmid T-DNA into the plants genome. See above for more details about ...
MULTISPECIES: hydantoinase/oxoprolinase family protein [Agrobacterium]. NCBI Reference Sequence: WP_012649231.1. Identical ... MULTISPECIES: hydantoinase/oxoprolinase family protein [Agrobacterium] MULTISPECIES: hydantoinase/oxoprolinase family protein [ ...
The method involves infection with Agrobacterium, particularly those comprising a super-binary vector. In this manner, ... cfu/ml Agrobacterium suspension (Agrobacterium suspended in PHI-I medium) and co-cultivated with Agrobacterium on PHI-J medium ... cfu/ml Agrobacterium suspension (Agrobacterium suspended in PHI-I medium) and co-cultivated with Agrobacterium on PHI-J medium ... cfu/ml Agrobacterium suspension (Agrobacterium suspended in PHI-I medium) and co-cultivated with Agrobacterium on PHI-J medium ...
The T-pilus of Agrobacterium tumefaciens.. Lai EM1, Kado CI.. Author information. 1. Davis Crown Gall Group, University of ... and protein-transport apparatus for the transport of cyclic T-pilin subunits to the Agrobacterium cell surface. T-pilin ... subunits are processed from full-length VirB2 pro-pilin into a cyclized peptide, a rapid reaction that is Agrobacterium ...
... to a method for the transformation of a monocot plant comprising exposing explant tissue of said monocot to an Agrobacterium ... The Agrobacterium is removed from the explant tissue, and an antibiotic against the Agrobacterium is added, and the transformed ... 0068] Introduction of pSOD-bar into Agrobacterium [0069] Competent Agrobacterium rumefaciens C58Cl cells, which contained the ... Agrobacterium rumefaciens strains, for example, but not lilted to, Agrobacterium strain C58. ...
Gelvin SB (2012) Traversing the cell: Agrobacterium T-DNAs journey to the host genome. Front Plant Sci 3:52PubMedGoogle ... Gelvin SB (2008) Agrobacterium-mediated DNA transfer, and then some. Nat Biotechnol 26:998-1000PubMedGoogle Scholar ... The strategy relies on Agrobacterium as a vector to deliver DNA copies of one or more viral RNA/DNA replicons; the bacteria are ... Magori S, Citovsky V (2011) Epigenetic control of Agrobacterium T-DNA integration. Biochim Biophys Acta 1809:388-394PubMed ...
Agrobacterium is the only cellular organism on Earth that is naturally capable of transferring genetic material between the ... The Oncogenes of Agrobacterium Tumefaciens and Agrobacterium Rhizogenes Monica T. Britton, Matthew A. Escobar, Abhaya M. ... Agrobacterium is a comprehensive book on Agrobacterium research, including its history, application, basic biology discoveries ... Agrobacterium DNA Expression Pathogen Pathogene Transport biotechnology crown gall disease currentjks gene transfer genetic ...
Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium; ... Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58. ...
This volume reviews various facets of Agrobacterium biology, from modern aspects of taxonomy and bacterial ecology to ... Agrobacterium Biology. Book Subtitle. From Basic Science to Biotechnology. Editors. * Stanton B. Gelvin ... Agrobacterium Biology. From Basic Science to Biotechnology. Editors: Gelvin, Stanton B. (Ed.) ... Agrobacterium-mediated transformation is the most extensively utilized platform for generating transgenic plants, but modern ...
Unless specified otherwise, MP Biomedicals products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department ...
Agrobacterium agile, Agrobacterium albertimagni, Agrobacterium aurantiacum, Agrobacterium larrymoorei, Agrobacterium ... radiobacter, Agrobacterium rhizogenes, Agrobacterium rubi, Agrobacterium tumefaciens, Agrobacterium vitis, Agrobacterium sp. ... Agrobacterium tumefaciens is a Gram-negative, non-sporeforming, rod-shaped bacterium. Agrobacterium strains use different ... Agrobacterium tumefaciens str. C58 has an unusual chromosomal organization - it has a 2 Mb linear and a 2.8 Mb circular ...
This proteome is part of the Agrobacterium radiobacter pan proteome (fasta) Agrobacterium are Gram-negative, motile, soil- ... More recently Agrobacterium have been classified into 3 biovars based on physiological and biochemical phenotypes without ... "Genome sequences of three Agrobacterium biovars help elucidate the evolution of multichromosome genomes in bacteria.". Slater S ...
Agrobacterium tumefaciens is a Gram-negative soil bacterium that causes plant tumours by transferring a portion of DNA from a ...
Association of single-stranded transferred DNA from Agrobacterium tumefaciens with tobacco cells.. V M Yusibov, T R Steck, V ... Association of single-stranded transferred DNA from Agrobacterium tumefaciens with tobacco cells. ... Association of single-stranded transferred DNA from Agrobacterium tumefaciens with tobacco cells. ... Association of single-stranded transferred DNA from Agrobacterium tumefaciens with tobacco cells. ...
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Certain plant-associated prokaryotes such as Corynebacteriurn fascians and Agrobacterium tumefaciens are known to produce ... Cytokinin production by Agrobacterium tumefaciens Public Deposited Citeable URL:. http://ir.library.oregonstate.edu/concern/ ...
Unless specified otherwise, MP Biomedicals products are for laboratory research use only, not for human or clinical use. For more information, please contact our customer service department ...
Glycoside Hydrolase Genes Are Required for Virulence of Agrobacterium tumefaciens on Bryophyllum daigremontiana and Tomato A. ... Expression of a Peptidoglycan Hydrolase from Lytic Bacteriophages Atu_ph02 and Atu_ph03 Triggers Lysis of Agrobacterium ...
Agrobacterium rhizogenes, Agrobacterium rubi, Agrobacterium tumefaciens, Agrobacterium vitis, Agrobacterium sp. ,h2> ... Agrobacterium agile, Agrobacterium albertimagni, Agrobacterium aurantiacum, Agrobacterium larrymoorei, Agrobacterium ... Interestingly, if Agrobacterium is grown near its maximum temperature of about 30,sup>o,/sup>C, then the plasmid is lost as ... Agrobacterium tumefaciens causes crown gall disease by first transferring part of its DNA into an opening in the plant. ...
2-Oxocarboxylic acids, also called 2-oxo acids and alpha-keto acids, are the most elementary set of metabolites that includes pyruvate (2-oxopropanoate), 2-oxobutanoate, oxaloacetate (2-oxosuccinate) and 2-oxoglutarate. This diagram illustrates the architecture of chain extension and modification reaction modules for 2-oxocarboxylic acids. The chain extension module RM001 is a tricarboxylic pathway where acetyl-CoA derived carbon is used to extend the chain length by one. The chain modification modules RM002 (including RM032) and RM033, together with a reductive amination step (RC00006 or RC00036), generate basic and branched-chain amino acids, respectively. The modification module RM030 is used in the biosynthesis of glucosinolates, a class of plant secondary metabolites, for conversion to oxime followed by addition of thio-glucose moiety. Furthermore, the chain extension from 2-oxoadipate to 2-oxosuberate is followed by coenzyme B biosynthesis in methonogenic archaea ...
"Sequence and mutational analysis of a tartrate utilization operon from Agrobacterium vitis.". Crouzet P., Otten L.. J. ... sp,Q44471,TTUC1_AGRVI Probable tartrate dehydrogenase/decarboxylase TtuC OS=Agrobacterium vitis OX=373 GN=ttuC PE=2 SV=1 ... cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium ... Agrobacterium vitis (Rhizobium vitis). ,p>This subsection of the ,a href="http://www.uniprot.org/help/names_and_taxonomy_ ...
The complexity of Agrobacterium-plant interaction has been studied for several decades. Agrobacterium pathogenicity is largely ... The complexity of Agrobacterium-plant interaction has been studied for several decades. Agrobacterium pathogenicity is largely ... Here we outline the responses of Agrobacterium to major plant-derived signals that impact Agrobacterium-plant interactions. ... Agrobacterium utilizes opines as nutrient sources as well as signals in order to activate its quorum sensing (QS) to further ...
Constitutive mutations of Agrobacterium tumefaciens transcriptional activator virG.. G J Pazour, C N Ta, A Das ... Constitutive mutations of Agrobacterium tumefaciens transcriptional activator virG. Message Subject (Your Name) has forwarded a ... The virulence (vir) genes of Agrobacterium tumefaciens Ti plasmids are positively regulated by virG in conjunction with virA ...
PubMed journal article Characterization of the VirG binding site of Agrobacterium tumefacien were found in PRIME PubMed. ... Dual control of Agrobacterium tumefaciens Ti plasmid virulence genes.. *Analysis of the Ros repressor of Agrobacterium virC and ... Expression of Agrobacterium tumefaciens virulence (vir) genes is dependent on the presence of a conserved vir box sequence in ... Pazour GJ, Das A. Characterization of the VirG Binding Site of Agrobacterium Tumefaciens. Nucleic Acids Res. 1990 Dec 11;18(23 ...
... Author(s). Wang, Yue; Kronenburg-van de Ven ... Calli of eight randomly chosen cultivars were co-cultivated with Agrobacterium tumefaciens strain AGL0 carrying binary vectors ...
  • The closely related species, Agrobacterium rhizogenes , induces root tumors, and carries the distinct Ri (root-inducing) plasmid. (wikipedia.org)
  • Strains within Agrobacterium tumefaciens and Agrobacterium rhizogenes are known to be able to harbour either a Ti or Ri- plasmid , whilst strains of Agrobacterium vitis , generally restricted to grapevines, can harbour a Ti-plasmid. (wikipedia.org)
  • Non- Agrobacterium strains have been isolated from environmental samples which harbour a Ri-plasmid whilst laboratory studies have shown that non- Agrobacterium strains can also harbour a Ti-plasmid. (wikipedia.org)
  • Some environmental strains of Agrobacterium possess neither a Ti nor Ri-plasmid. (wikipedia.org)
  • T-pilin subunits are processed from full-length VirB2 pro-pilin into a cyclized peptide, a rapid reaction that is Agrobacterium specific and can occur in the absence of Ti-plasmid genes. (nih.gov)
  • Interestingly, if Agrobacterium is grown near its maximum temperature of about 30 o C, then the plasmid is lost as well as the pathogenicity of the bacterium. (kenyon.edu)
  • Agrobacterium tumefaciens is a Gram-negative soil bacterium that causes plant tumours by transferring a portion of DNA from a resident 'tumour inducing' (Ti) plasmid into plant cells where it is integrated into a plant chromosome and expressed. (wiley.com)
  • During the inception of crown gall tumorigenesis, the transferred DNA (T-DNA) is processed from the Ti (tumor inducing) plasmid of Agrobacterium tumefaciens and is transferred to plant cells. (pnas.org)
  • Agrobacterium perceives plant-derived signals to activate its virulence genes, which are responsible for transferring and integrating its Transferred DNA (T-DNA) from its Tumor-inducing (Ti) plasmid into the plant nucleus. (frontiersin.org)
  • In addition, Agrobacterium hijacks plant-derived signals including SA, IAA, and ethylene to down-regulate its virulence genes located on the Ti plasmid. (frontiersin.org)
  • Upon detection of plant-derived signals in the rhizosphere, Agrobacterium activates its chromosomal virulence genes ( chv genes) and Ti plasmid encoded virulence genes ( vir genes). (frontiersin.org)
  • We assessed the impact of seed inoculation with the emblematic bacterial models Agrobacterium tumefaciens C58 (plasmid-cured) or Escherichia coli K-12 on maize seedlings in nonsterile soil. (apsnet.org)
  • The IncW plasmid pSa originally derived from Shigella flexneri completely inhibits the tumor-inducing ability of Agrobacterium tumefaciens when it is resident in this organism. (asm.org)
  • Using this method, the pathogenic genes of Agrobacterium tumefaciens T-DNA in the Ti plasmid are replaced with selective marker genes as well as the exogenous target gene. (cellartis.com)
  • The LBA4044 strain of Agrobacterium tumefaciens contains the pAL4404 plasmid, which includes only the T-DNA vir region and enables only vir gene induction and T-DNA transfer. (cellartis.com)
  • Agrobacterium tumefaciens (Rhizobium radiobactor) can transfer T-DNA (transfer DNA), which is part of its own Ti plasmid, into host plant cells and insert this DNA into the plant chromosomal DNA. (cellartis.com)
  • In this system, the pathogenic genes of T-DNA in Ti plasmid are replaced with selective marker genes and the exogenous target gene, to transfer the target gene into plant chromosomal DNA by means of Agrobacterium -mediated gene transfer. (cellartis.com)
  • Hoekema, A. Hirsch, P.R. Hooykaas, P.J.J. Schilperoort, R.A. A binary plant vector strategy based on separation of vir - and T-region of the Agrobacterium tumefaciens Ti-plasmid. (cellartis.com)
  • Octopine Ti-plasmid deletion mutants of Agrobacterium tumefaciens with emphasis on the right side of the T-region. (cellartis.com)
  • Previously I cloned my sequence into agrobacterium plasmid as later on I need to use it to infect my plants. (nucleics.com)
  • But when I extracted the agrobacterium plasmid and sent it for sequencing, the result was terrible. (nucleics.com)
  • These inflorescences were dipped in an infiltration solution of Agrobacterium tumefaciens GV3101 carrying the pMP90RK plasmid. (jove.com)
  • When Agrobacterium tumefaciens is used to introduce new genes to plants, scientists remove the plasmid genes that induce tumors but retain those that are needed for the transfer. (northernwoodlands.org)
  • Koc, N. 2006-05-15 00:00:00 Cotton (Gossypium hirsutum L.) was transformed by the EHA101 strain of Agrobacterium tumefaciens harboring a binary vector pGA482GG plasmid carrying the marker genes for neomycin phosphotransferase II (nptII) determining resistance to kanamycin and β-glucuronidase (GUS). (deepdyve.com)
  • Intermediate vectors, containing specific Ti plasmid sequences and capable of replication in both E. coli and Agrobacterium strains, were constructed and used for the in vitro introduction of isolated DNA fragments into. (ugent.be)
  • The phytopathogenic bacteria Agrobacterium rhizogenes genetically transforms plants by transferring a portion of the resident Ri plasmid, the T-DNA to the plant. (ejbiotechnology.info)
  • A number of methods were employed to detect in situ vir-induction and, to this end, genes encoding β-glucuronidase (GUS) and bioluminescence (lux) were linked in plasmid constructs to Agrobacterium vir-promoters. (dur.ac.uk)
  • A common transformation protocol for Arabidopsis is the floral dip method: inflorescence are dipped in a suspension of Agrobacterium, and the bacterium transforms the germline cells that make the female gametes. (wikipedia.org)
  • Agrobacterium tumefaciens is a Gram-negative, non-sporeforming, rod-shaped bacterium. (kenyon.edu)
  • As a ubiquitous soil bacterium, Agrobacterium is capable of two lifestyles: independent free-living or acting as a pathogen in association with a plant host. (frontiersin.org)
  • However, until about 20 years ago, it was generally believed that monocotyledons could not be transformed by Agrobacterium, because these plants are outside the host range of crown gall disease caused by this bacterium. (frontiersin.org)
  • Arabidopsis efr mutants show enhanced susceptibility to the bacterium Agrobacterium tumefaciens, as revealed by a higher efficiency of T-DNA transformation. (nih.gov)
  • Described herein is the first reported case of prosthetic valve endocarditis caused by Agrobacterium radiobacter , a gram-negative soil bacterium. (annals.org)
  • The polarly growing plant pathogen Agrobacterium tumefaciens was used as a model bacterium to explore these polar growth mechanisms. (asm.org)
  • In 1982, Chilton and her team harnessed the gene-transfer mechanism of the bacterium, Agrobacterium, to produce a transgenic plant. (croplife.com)
  • The plant-growth-promoting bacterium Agrobacterium tumefaciens CCNWGS0286, isolated from the nodules of Robinia pseudoacacia growing in zinc-lead mine tailings, both displayed high metal resistance and enhanced the growth of Robinia plants in a metal-contaminated environment. (rti.org)
  • Agrobacterium tumefaciens is a bacterium which, with the help of the Ti megaplasmid, manipulates plants into providing it with a safe and nutritious environment. (splasho.com)
  • One of the earliest associations of human disease caused by Agrobacterium radiobacter was reported by Dr. J. R. Cain in Scotland (1988). (wikipedia.org)
  • Agrobacterium tumefaciens (updated scientific name Rhizobium radiobacter, synonym Agrobacterium radiobacter) is the causal agent of crown gall disease (the formation of tumours) in over 140 species of eudicots. (wikipedia.org)
  • Agrobacterium radiobacter grows on various explosives such as nitroglycerine - they use this as their sole source of nitrogen. (kenyon.edu)
  • A. tumefaciens is also known as Rhizobium radiobacter, and Agrobacterium radiobacter. (wardsci.com)
  • PLOTKIN GR. Agrobacterium radiobacter Prosthetic Valve Endocarditis. (annals.org)
  • Agrobacterium radiobacter is a sample topic from the Taber's Medical Dictionary . (tabers.com)
  • Taber's Medical Dictionary, Taber's Online, https://www.unboundmedicine.com/medline/view/Tabers-Dictionary/741040/all/Agrobacterium_radiobacter. (tabers.com)
  • A particular strain of a close relative of crown gall, called Agrobacterium radiobacter , protects plants from its gall-forming cousin. (northernwoodlands.org)
  • Agrobacterium utilizes opines as nutrient sources as well as signals in order to activate its quorum sensing (QS) to further promote virulence and opine metabolism. (frontiersin.org)
  • Moreover, certain metabolites from corn ( Zea mays ) also inhibit the expression of Agrobacterium virulence genes. (frontiersin.org)
  • When living independently, Agrobacterium virulence is essentially silent. (frontiersin.org)
  • The virulence (vir) genes of Agrobacterium tumefaciens Ti plasmids are positively regulated by virG in conjunction with virA and plant-derived inducing molecules. (asm.org)
  • Expression of Agrobacterium tumefaciens virulence (vir) genes is dependent on the presence of a conserved 'vir box' sequence in their 5' nontranscribed regions. (unboundmedicine.com)
  • VL - 18 IS - 23 N2 - Expression of Agrobacterium tumefaciens virulence (vir) genes is dependent on the presence of a conserved 'vir box' sequence in their 5' nontranscribed regions. (unboundmedicine.com)
  • Hooykaas, P.J.J. & Beijersbergen, G.M. 1994 The virulence system of Agrobacterium tumefaciens . (wiley.com)
  • Instead, these processes are likely mediated by two Agrobacterium Virulence (Vir) proteins, VirD2 and VirE2, that are thought to associate directly with the T-strand, forming a transport (T) complex (97). (purdue.edu)
  • Virulence genes of Agrobacterium tumefaciens are induced in parallel in the presence of plant phenolic compounds such as acetosyringone and the two regulatory vir genes virA and virG. (unboundmedicine.com)
  • VL - 172 IS - 2 N2 - Virulence genes of Agrobacterium tumefaciens are induced in parallel in the presence of plant phenolic compounds such as acetosyringone and the two regulatory vir genes virA and virG. (unboundmedicine.com)
  • Construction of Agrobacterium strains by electroporation of genomic DNA and its utility in analysis of chromosomal virulence mutations. (asm.org)
  • Here we performed immunofluorescence deconvolution microscopy to localize the assembled T4SS by detection of its native components VirB1, VirB2, VirB4, VirB5, VirB7, VirB8, VirB9, VirB10, and VirB11 in the C58 nopaline strain of Agrobacterium tumefaciens , following induction of virulence ( vir ) gene expression. (asm.org)
  • The aim of this work was twofold: to construct plasmids with a gene encoding a pesticidal protein expressed from an Agrobacterium tumefaciens virulence promoter and to determine, in planta, the sites of Agrobacterium vir-induction. (dur.ac.uk)
  • The present method is directed to a method for the transformation of a monocot plant comprising exposing explant tissue of said monocot to an Agrobacterium strain under vacuum in the presence of a phenolic compound, said Agrobacterium strain comprising a heterologous gene of interest within a vector. (freepatentsonline.com)
  • Calli of eight randomly chosen cultivars were co-cultivated with Agrobacterium tumefaciens strain AGL0 carrying binary vectors with the gus gene as reporter and putative transgenic plants were produced. (wur.nl)
  • Agrobacterium tumefaciens strain LBA4404 and the binary vector method were invented by Dr. P.J. Hooykaas at Leiden University in the Netherlands. (cellartis.com)
  • Agrobacterium tumefaciens LBA4044 electrocompetent cells are a widely used bacterial strain for T-DNA transfer to plant cells. (cellartis.com)
  • Lady Olympia, Granola, Agria, Désirée and Innovator were infected with Agrobacterium tumefaciens strain LBA4404 containing pBIN19 expression vector with β-glucuronidase gusA gene under the control of 35S CaMV promoter. (srce.hr)
  • The Agrobacterium strain used was LBA4404 carrying a `Super-binary' vector with a bar gene as a selectable marker for herbicide resistance in the plant cells. (deepdyve.com)
  • Root explants were transformed by co-cultivation with Agrobacterium tumefaciens strain LBA4404 harbouring a binary vector pBAL2 carrying the reporter gene β-glucuronidase intron (GUS-INT) and the marker gene neomycin phosphotransferase (NPTII). (omicsonline.org)
  • Jouanin L, Guerche P, Pamboukdjian N, Tourneur C, Casse-Delbart F, Tourneur J (1987) Structure of T-DNA in plants regenerated from roots transformed by Agrobacterium rhizogenes strain A4. (springer.com)
  • Embryonic tip explants of 92 Indian soyabean and 7 advanced breeding lines derived from soaked mature seeds were inoculated and co-cultivated for 5-day with Agrobacterium strain EHA105 carrying the binary vector pCambia1305.1 containing a hygromycin and kanamycin resistance gene as plant and bacterial selectable markers, respectively. (banglajol.info)
  • Transformation of four soybean cultivars (Nannong88-1, Nannong18-6, Yu23 and Nannong 87C-38) by infecting cotyledonary-node with Agrobacterium tumefaciens strain EHA105 harboring pBI121 containing GFP reporter gene was conducted. (ajol.info)
  • Bar gene coding Glufosinate resistance is transformed into the alfalfa variety, Caoyuan No.1, mediated by Agrobacterium tumefaciens. (cnki.com.cn)
  • Recent taxonomic studies have reclassified all of the Agrobacterium species into new genera, such as Ahrensia , Pseudorhodobacter , Ruegeria , and Stappia , [1] [2] but most species have been controversially reclassified as Rhizobium species. (wikipedia.org)
  • Agrobacterium tumefaciens ( Rhizobium radiobactor ) is capable of T-DNA transfer to plant cells. (cellartis.com)
  • Although the pRI 101 DNA vectors are shuttle vectors and replicate autonomously in E. coli and Rhizobium ( Agrobacterium ), they are high copy number plasmids because they contain the same replication origin as pUC-type plasmids (ColE1 ori). (clontech.com)
  • These vectors are also stably maintained in Rhizobium ( Agrobacterium ) containing the mutant-type replication origin Ri (Ri-ori). (clontech.com)
  • Antisera raised against 50S ribosomal subunits of five different agrobacteria were used to arrange 31 Agrobacterium strains and 6 Rhizobium strains into 16 serovars. (oregonstate.edu)
  • Agrobacterium rhizogenes (or Rhizobium rhizogenes ) is able to transform plant genomes and induce the production of hairy roots. (plantphysiol.org)
  • Agrobacterium or Rhizobium, which name to use? (rhizobia.co.nz)
  • Agrobacterium is a well-known genus in bacteriology and molecular biology, but research has shown that it cannot easily be separated from the Rhizobium genus, thus all Agrobacterium species should be renamed as Rhizobium species (the earlier name). (rhizobia.co.nz)
  • It was also found that the biological lifestyles of symbiotic root nodule nitrogen fixation and pathogenicity were controlled by mobile DNA elements such as plasmids, and that an ' Agrobacterium' species could be changed into a ' Rhizobium' species (and vice versa) by manipulation of these mobile genes (e.g. (rhizobia.co.nz)
  • formally proposing to make Agrobacterium a synonym of Rhizobium ( Rhizobium has priority as the earlier described name). (rhizobia.co.nz)
  • who agreed that Agrobacterium was polyphyletic, but supported the retention of Agrobacterium , largely on the basis that they have different phenotypic traits from Rhizobium species. (rhizobia.co.nz)
  • A proposal was made in 2011 by the ' Subcommittee on the taxonomy of Agrobacterium and Rhizobium ' to retain the Agrobacterium genus by only transferring the more distantly related species, A. rhizogenes , to Rhizobium . (rhizobia.co.nz)
  • Currently there is some confusion over whether Agrobacterium or Rhizobium is the best name to use for these bacteria. (rhizobia.co.nz)
  • Although the taxonomic basis for the reclassification of Agrobacterium to Rhizobium is supported by some bacterial systematists (e.g. (rhizobia.co.nz)
  • The proposal by the 'Subcommittee on the taxonomy of Agrobacterium and Rhizobium' would seem to provide a solution by emending the concept of Agrobacterium to a more restricted sense. (rhizobia.co.nz)
  • In my opinion there is insufficient evidence that Agrobacterium as a genus can be clearly distinguished from Rhizobium , and thus the two names are synonyms. (rhizobia.co.nz)
  • There is no difference in Agrobacterium and Rhizobium, they are synonymous no doubt. (rhizobia.co.nz)
  • Although the taxonomy of Agrobacterium is currently under revision it can be generalised that 3 biovars exist within the genus, Agrobacterium tumefaciens , Agrobacterium rhizogenes , and Agrobacterium vitis . (wikipedia.org)
  • Infection by the species Agrobacterium vitis results in cane gall on grapevines while A. rhizogenes causes excessive formation of hairy roots or root tumors. (frontiersin.org)
  • Opines are carbon compounds produced by crown galls and hairy roots induced by Agrobacterium tumefaciens and A. rhizogenes , respectively. (apsnet.org)
  • Gene expression of reporters is indistinguishable in plants transformed by Agrobacterium tumefaciens as compared with A. rhizogenes . (plantphysiol.org)
  • Ambros PF, Matzke AJM, Matzke MA (1986) Localization of Agrobacterium rhizogenes T-DNA in plant chromosomes by in situ hybridization. (springer.com)
  • Guerche P, Jouanin L, Tepfer D, Pelletier G (1987) Genetic transformation of oilseed rape ( Brassica napus ) by the Ri T-DNA of Agrobacterium rhizogenes and analysis of inheritance of the transformed phenotype. (springer.com)
  • Hamill JD, Parr AJ, Robins RJ, Rhodes MJC (1986) Secondary product formation by cultures of Beta vulgaris and Nicotiana rustica transformed with Agrobacterium rhizogenes. (springer.com)
  • Handa T (1990) Hairy root culture and plant regeneration of various plant species by Agrobacterium rhizogenes . (springer.com)
  • Mano Y, Ohkawa H, Yamada Y (1989) Production of tropane alkaloids by hairy root cultures of Duboisia leichhardtii transformed by Agrobacterium rhizogenes . (springer.com)
  • Agrobacterium rhizogenes mediated transformation efficiency was assessed under the influence of sonication, calcium treatment, acetosyringone and macerating enzymes in suitable combinations in Nicotiana tabacum as a model system. (ejbiotechnology.info)
  • The results of this study may be very useful in genetic manipulation of plants by Agrobacterium rhizogenes mediated gene delivery to higher plants, which are recalcitrant to A. tumefaciens mediated genetic manipulation. (ejbiotechnology.info)
  • Among them, most widely studied ones are Agrobacterium tumefaciens and Agrobacterium rhizogenes . (ejbiotechnology.info)
  • Many reports suggest the use of Agrobacterium rhizogenes for expression of the rol genes and also to deliver foreign genes to susceptible plants ( Christey, 2001 ). (ejbiotechnology.info)
  • [11] A later study suggested that Agrobacterium attaches to and genetically transforms several types of human cells by integrating its T-DNA into the human cell genome. (wikipedia.org)
  • 1. An Agrobacterium-transformed sorghum plant wherein said plant comprises fewer than 5 copies of a nucleic acid of interest flanked by at least one T-DNA border sequence incorporated in its genome. (freepatentsonline.com)
  • The genome of the natural genetic engineer Agrobacterium tumefaciens C58. (genome.jp)
  • Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58. (genome.jp)
  • The genome of Agrobacterium vitis is currnetly being sequenced. (kenyon.edu)
  • Genome sequence analysis revealed homologs of nitrite reductase genes nirK and nirBD and siderophore synthesis genes for Agrobacterium tumefaciens , as well as homologs of nitrite reductase genes nirBD and phosphatase genes phoA and appA in E. coli , whose contribution to phytostimulation will require experimental assessment. (apsnet.org)
  • KEGG GENOME: Agrobacterium sp. (kegg.jp)
  • To better understand polar growth in the Rhizobiales Agrobacterium tumefaciens , we first surveyed its genome to identify homologs of (~70) well-known PG synthesis components. (asm.org)
  • Agrobacterium tumefaciens is a plant pathogen with the unique ability to transfer a defined segment of DNA to eukaryotes, where it integrates into the eukaryotic genome. (sciencemag.org)
  • Based on the positive results of the study, it has proven that the Agrobacterium -delivered CRISPR-Cas9 system could be used for wheat genome editing, which only needs a small number of transformation events. (isaaa.org)
  • And most GFP-positive plants were confirmed to be positive by Southern blot analysis, which showed that transformation of cotyledonary-node explants mediated by Agrobacterium delivered T-DNA with one or two copies into soybean genome. (ajol.info)
  • Agrobacterium is well known for its ability to transfer DNA between itself and plants, and for this reason it has become an important tool for genetic engineering . (wikipedia.org)
  • The ability of Agrobacterium to transfer genes to plants and fungi is used in biotechnology , in particular, genetic engineering for plant improvement . (wikipedia.org)
  • Agrobacterium is the only cellular organism on Earth that is naturally capable of transferring genetic material between the kingdoms of life, from prokaryotes to eukaryotes. (springer.com)
  • Studies have uncovered a wealth of information on the process of Agrobacterium-mediated genetic transformation and on the bacterial and host cell factors involved in the infection. (springer.com)
  • It is therefore not surprising that Agrobacterium and the genetic transformation itself have also become the focus of numerous ethical and legal debates. (springer.com)
  • The result is an all-inclusive text which readers-including scientists and students involved in plant genetic engineering-will find useful as a reference source for all major aspects of the Agrobacterium-mediated genetic transformation of plant and non-plant organisms. (springer.com)
  • Agrobacterium-mediated transformation and direct gene transfer using the gene gun (microparticle -bombardment) are the two most widely used methods for plant genetic modification. (biomedsearch.com)
  • The use of Agrobacterium tumefaciens-mediated transformation for achieving genetic transformation of fungi has steadily increased over the last decade, and has proven to be almost universally applicable technique once suitable selection markers have been developed. (dtu.dk)
  • Sarker, R.H. and Biswas, A. (2002) In Vitro Plantlet Regeneration and Agrobacterium-Mediated Genetic Transformation of Wheat (Triticum aestivum L.) Plant Tissue Culture, 12, 155-165. (scirp.org)
  • An efficient variety-independent method for producing transgenic Black night shade (Solanum nigrum L.) via Agrobacterium tumefaciens-mediated genetic transformation was developed. (omicsonline.org)
  • Thus, a protocol for effective Agrobacterium-mediated genetic transformation of cotton was optimized. (deepdyve.com)
  • A high genetic variability for Agrobacterium -infection ranging from 3.8 to 100% was observed in the form of transient GUS expression. (banglajol.info)
  • We have extended the technique of electroporation as a genetic tool for manipulating the Agrobacterium tumefaciens chromosome. (asm.org)
  • Agrobacterium tumefaciens is the most commonly studied species in this genus. (wikipedia.org)
  • Unlike the nitrogen-fixing symbionts, tumor-producing Agrobacterium species are pathogenic and do not benefit the plant. (wikipedia.org)
  • Agrobacterium has been shown to genetically transform, under laboratory conditions a large number of plant species and numerous non-plant organisms, indicating the truly basic nature of the transformation process. (springer.com)
  • Agrobacterium are Gram-negative, motile, soil-dwelling plant pathogens with the species name given based on the disease phenotype associated with the bacteria. (uniprot.org)
  • Agrobacterium is a genus of Gram-negative bacteria that uses horizontal gene transfer to cause tumors in many plant species with agricultural and economic importance including woody ornamental shrubs (rose), vines (grape), shade trees, fruit trees (cherry, berry, walnut), and herbaceous perennials. (frontiersin.org)
  • A dual-species cocultivation model has been developed by using two ubiquitous and well studied microbes Pseudomonas aeruginosa ( P.a. ) and Agrobacterium tumefaciens ( A.t. ) as a tractable system to identify molecular mechanisms that underlie multispecies microbial associations. (pnas.org)
  • To examine this hypothesis, we have developed a dual-species model system, composed of Pseudomonas aeruginosa ( P.a. ) and Agrobacterium tumefaciens ( A.t. ). (pnas.org)
  • Agrobacterium tumefaciens-mediated gene transfer is a method employed widely in many plant species. (frontiersin.org)
  • Agrobacterium tumefaciens is the preferred method for transformation of a wide range of plant species. (nih.gov)
  • The protocol we follow for Agrobacterium -mediated transformation of both cultivated and wild species of tomato is detailed in this chapter. (springer.com)
  • Agrobacterium tumefaciens elicits neoplastic growths on many plant species (15). (purdue.edu)
  • Although plants represent the natural hosts for Agrobacterium , this microorganism can also transform a wide range of other eukaryotic species, from fungi (16, 62) to human cells (48). (purdue.edu)
  • Biologists frequently tweak the age-old procedures of Agrobacterium tumefaciens to create remodeled crop species. (northernwoodlands.org)
  • Flax (Linum usitatissimum L.), like most dicotyledonous crop species, is amenable to gene transfer via Agrobacterium, and it is also readily regenerated in vitro. (eurekamag.com)
  • The genus Agrobacterium includes phytopathogenic bacteria that induce the development of root crown galls and/or aerial galls at the base of the stem or hairy roots on more than 600 species of plants belonging to 90 dicotyledonous families and non-pathogenic species. (scielo.org.ar)
  • Therefore, the combination of multiplex PCR, specifc PCR, isolations in semi-selective D1, D1-M and YEM-RCT media combined with bioassays on cut leaves of Kalanchoe and seedlings of California Wonder pepper cultivar constitute an accurate tool to detect species and biovars of Agrobacterium for diagnostic purposes. (scielo.org.ar)
  • We used a phylogenetic approach accounting for the transfer of genes (or groups of genes) to estimate the history of genomes in Agrobacterium biovar 1, a diverse group of soil and plant-dwelling bacterial species. (pasteur.fr)
  • These factors may be the result of, or linked to, hypersensitive defense reaction in plants to Agrobacterium infection, which may involve the recognition of specific signals from the Agrobacterium that triggers the burst of reactive oxygen species at the infection site. (ajol.info)
  • Evidences of Agrobacterium -induced necrosis in target plant tissues and its link to reactive oxygen species are presented. (ajol.info)
  • Although generally seen as an infection in plants, Agrobacterium can be responsible for opportunistic infections in humans with weakened immune systems , [9] [10] but has not been shown to be a primary pathogen in otherwise healthy individuals. (wikipedia.org)
  • The method involves infection with Agrobacterium, particularly those comprising a super-binary vector. (freepatentsonline.com)
  • Transformation efficiency was enhanced by optimizing the Agrobacterium concentration (OD 600 = 0.8-1.0) and an infection time (20-30 min). (mdpi.com)
  • The transformation protocol routinely used in our laboratory has been applied to many different tomato genotypes and relies on Agrobacterium tumefaciens infection of young cotyledon sections. (springer.com)
  • When plants are being grafted en masse in nurseries, precautions against crown gall infection are essential. (northernwoodlands.org)
  • Agrobacterium tumefaciens causes crown-gall disease in plants. (wikipedia.org)
  • Agrobacterium tumefaciens causes crown gall disease by first transferring part of its DNA into an opening in the plant. (kenyon.edu)
  • agrobacterium causes crown gall disease in humans and it's called morgellons. (croplife.com)
  • Forty-four independent transformed tobacco plants were obtained from a cocultivation experiment with Agrobacterium tumefaciens strains carrying modified Ti-plasmids. (genetics.org)
  • OSCAR (One Step Construction av Agrobacterium-Recombination-ready-plasmids) -metoden för snabb generering av deletionskonstruktioner beskrivs. (jove.com)
  • Gel electrophoresis allowed the detection of plasmids in bacteria at this time, and scientists attempted to isolate them from Agrobacterium. (splasho.com)
  • This volume reviews various facets of Agrobacterium biology, from modern aspects of taxonomy and bacterial ecology to pathogenesis, bacterial cell biology, plant and fungal transformation, natural transgenics, and biotechnology. (springer.com)
  • Agrobacterium -plant interaction is an excellent paradigm for studying both plant and bacterial responses, as well as the role of chemical signaling in these processes. (frontiersin.org)
  • In Agrobacterium, the T-region is delimited by two 25-bp direct repeats, the T-DNA borders, that are cleaved by the bacterial VirD2 endonuclease to produce a transferable T-strand molecule (86). (purdue.edu)
  • An inducible marker system suitable for Agrobacterium tumefaciens was constructed to enable detection and enumeration of specific bacterial cells introduced into soil. (eurekamag.com)
  • Agrobacterium is a genus of Gram-negative bacteria established by H. J. Conn that uses horizontal gene transfer to cause tumors in plants . (wikipedia.org)
  • Comparison between agrobacterium-mediated and direct gene transfer using the gene gun. (biomedsearch.com)
  • It also carries genes for the biosynthesis of the plant hormones , auxin and cytokinins , and for the biosynthesis of opines , providing a carbon and nitrogen source for the bacteria that most other micro-organisms can't use, giving Agrobacterium a selective advantage . (wikipedia.org)
  • Scanning electron micrograph (SEM), tinted green, of the bacteria Agrobacterium tumifaciens (small bacilli) growing on the surface of cells from the tobacco plant Nicotiana plumbaginifolia. (sciencephoto.com)
  • The Agrobacterium genus was described by Conn in 1942 to encompass plant pathogenic bacteria that caused galls and root disease . (rhizobia.co.nz)
  • Chyi YS, Phillips GC (1987) High efficiency Agrobacterium -mediated transformation of Lycopersicon based on conditions favorable for regeneration. (springer.com)
  • Does anyone else working with Agrobacterium tumefaciens have the problems that I do? (bio.net)
  • Liu, S. Efficient Agrobacterium -Mediated Transformation of Hybrid Poplar Populus davidiana Dode × Populus bollena Lauche. (mdpi.com)
  • According to transmission electron microscopy observations, there were more Agrobacterium invasions in the 30-day-old leaf explants than in 60-day-old and 90-day-old explants. (mdpi.com)
  • An optimized procedure, based on transformation efficiencies (measured as percentage of transformed explants and average gfp spots per transformed explant) and control of the Agrobacterium growth after co-culture, was established. (csic.es)
  • Dong, L.-C. , Sun, C.-W. , Thies, K.L. , Luthe, D.S. & Graves, C.H. Jr 1992 Use of polymerase chain reaction to detect pathogenic strains of Agrobacterium . (wiley.com)
  • Among the pathogenic Agrobacterium strains isolated from 18 galled trees from six counties, biovar 1 strains predominated and most were sensitive to agrocin 84 in vitro. (oregonstate.edu)
  • Frandsen, RJN 2015, Agrobacterium tumefaciens -Mediated Transformation . (dtu.dk)
  • Agrobacterium tumefaciens‐mediated transformation of maize embryos using a standard binary vector system. (currentprotocols.com)
  • Agrobacterium tumefaciens-mediated transformation was successfully applied to P. fumosoroseus. (aspergillus.org.uk)
  • Plant viral vectors delivered by Agrobacterium are the basis of several manufacturing processes that are currently in use for producing a wide range of proteins for multiple applications, including vaccine antigens, antibodies, protein nanoparticles such as virus-like particles (VLPs), and other protein and protein-RNA scaffolds. (springer.com)
  • Pierce, Dortie 2004-10-16 00:00:00 Agrobacterium tumefaciens was used to genetically transform sorghum. (deepdyve.com)
  • In this research, the Colombian soybean varieties SK7, p29 and Soyica p34 ability to be genetically transformed by Agrobacterium tumefaciens strains AGL0 and EHA105 using a Glyphosate tolerance cassette was tested. (scielo.org.co)
  • However there has been some opposition to renaming Agrobacterium , in this article I explain the research and taxonomy, and suggest a solution. (rhizobia.co.nz)
  • Several Agrobacterium tumefaciens strains have been isolated for their ability to produce D-amino acids from D, L-substituted hydantoins. (openthesis.org)
  • Agrobacterium-Mediated Transformation of Brachypodium distachyon. (annals.org)
  • Lazo GR, Stein PA, Ludwig RA (1991) A DNA transformation-competent Arabidopsis genomic library in Agrobacterium . (springer.com)
  • genes) results in loss of transformation proficiency of Arabidopsis (rat, resistance to Agrobacterium transformation, phenotype). (purdue.edu)
  • Therefore, this system is often used for Agrobacterium -mediated transformation of several dicots such as Arabidopsis thaliana , tobacco, potato and soybeans as well as monocots like corn. (goldbio.com)
  • Therefore, this system is often used for Agrobacterium -mediated transformation of Arabidopsis thaliana as well as maize and other monocots. (goldbio.com)
  • Genomes of plants and fungi can be engineered by use of Agrobacterium for the delivery of sequences hosted in T-DNA binary vectors. (wikipedia.org)
  • LOPEZ, Ana C y BALATTI, Pedro A . Methods for the detection of Agrobacterium from plant, soil and water samples. (scielo.org.ar)
  • In roots as well as in shoots, Agrobacterium tumefaciens and E. coli triggered similar (in PR37Y15) or different (in DK315) changes in the high-performance liquid chromatography profiles of secondary metabolites (especially benzoxazinoids), distinct from those of Azospirillum brasilense UAP-154. (apsnet.org)
  • Commonly, the genes to be transferred are cloned between the left and right T-DNA borders of so-called binary T-DNA vectors that can replicate both in E. coli and Agrobacterium. (nih.gov)
  • Agrobacterium -mediated transformation is the most extensively utilized platform for generating transgenic plants, but modern biotechnology applications derive from more than 40 years of intensive basic scientific research. (springer.com)
  • Razvoj učinkovitog i reproducibilnog postupka dobivanja pet stabilnih kultivara krumpira genetička transformacijom s pomoću bakterije Agrobacterium tumefaciens ', Food Technology and Biotechnology , 58(1), str. (srce.hr)
  • Agrobacterium tumefaciens is a plant pathogen capable of transferring a defined segment of DNA to a host plant, generating a gall tumor. (sciencemag.org)
  • Agrobacterium is known for its tumor forming abilities, which has been exploited by molecular biologist for transferring genes to plants. (rhizobia.co.nz)
  • Scientists know a lot now about virulent Agrobacterium strains do this and have worked out methods to use this n atural DNA transformation process to in troduce no vel genes into plants and even fungi for agricu l tural, medical , and bio technolog ical purposes. (google.com)
  • 6. The method of claim 2 wherein said Agrobacterium comprises a super virulent vector. (freepatentsonline.com)
  • Attachment of virulent Agrobacterium tumefaciens to plant cells is required for transformation. (plantphysiol.org)
  • GoldBio's GV3101 Agrobacterium Electrocompetent Cells are optimized for the highest transformation efficiencies which is ideal for applications requiring high transformation efficiencies, such as cDNA or gDNA library construction. (goldbio.com)
  • GV3101 Agrobacterium Electrocompetent cells should be stored at -80°C, pCAMBIA1391z Control DNA should be stored at -20°C and recovery medium should be stored at 4°C immediately upon arrival. (goldbio.com)
  • GoldBio's AGL-1 Agrobacterium Electrocompetent Cells are optimized to produce the highest transformation efficiency, and are ideal for applications requiring high transformation efficiencies such as cDNA or gDNA library construction. (goldbio.com)
  • The large growths on these roots are galls induced by Agrobacterium sp. (wikipedia.org)
  • As a special phytopathogen, Agrobacterium tumefaciens infects a wide range of plant hosts and causes plant tumors also known as crown galls. (frontiersin.org)
  • Central to the study and engineering of plants is their transformation, which is achieved most commonly using Agrobacterium tumefaciens , the causal agent of crown galls (or tumors) in dicotyledonous plants ( Smith and Townsend 1907 ). (g3journal.org)
  • This results in stable integration of the exogenous target gene into plant chromosomal DNA by means of Agrobacterium -mediated transformation. (cellartis.com)
  • At the turn of the century Agrobacterium tumefaciens was identified as the causal agent in crown gall disease in dicotyledonous plants. (kenyon.edu)
  • The genus Agrobacterium is quite heterogeneous . (wikipedia.org)
  • A. tumef aciens C58 is the most heavily studied member of the genus Agrobacterium . (google.com)
  • These agrobacterium-mediated plant transformation vectors carry the 35S promoter of caµliflower mosaic virus (CaMV) and the 5' non-coding region (5'-UTR) of the alcohol dehydrogenase (ADH) gene. (clontech.com)
  • A preculture time of 9-12 days prior to inoculation with a disarmed nopaline-type pTiT37 Agrobacterium tumefaciens yields the best recovery of transgenic plants. (eurekamag.com)
  • Protoplasts or alternatively leaf-discs can be incubated with the Agrobacterium and whole plants regenerated using plant tissue culture. (wikipedia.org)
  • In the presence of opines, A. tumefaciens produces a diffusible conjugation signal called 30C8HSL or the Agrobacterium autoinducer[citation needed]. (wikipedia.org)
  • Certain Agrobacterium strains were chemoattracted to opines. (apsnet.org)
  • Agrobacterium strains were attracted to several opines tested. (illinois.edu)
  • Agrobacterium strains use different carbohydrates and are classified into three main biovars. (kenyon.edu)
  • Two pantothenate-requiring mutants of Agrobacterium tumefaciens have been isolated. (unl.edu)