Acetylcholinesterase inhibition by flavonoids from Agrimonia pilosa. (1/5)

In a bioassay-guided search for acetylcholinesterase (AChE) inhibitors from 180 medicinal plants, an ethyl acetate extract of whole plants of Agrimonia pilosa ledeb yielded tiliroside (1), 3-methoxy quercetin (2), quercitrin (3) and quercetin (4). We report herein for the first time that all four flavonol compounds showed significant inhibitory effects on AChE, particularly quercetin (4), which showed twice the activity of dehydroevodiamine (DHED).  (+info)

Effect of neuroprotective flavonoids of Agrimonia eupatoria on glutamate-induced oxidative injury to HT22 hippocampal cells. (2/5)

A methanolic extract of Agrimonia eupatoria (Rosaceae) significantly attenuated glutamate-induced oxidative stress in HT22 hippocampal cells. A new flavonoid, characterized as kaempferol 3-O-beta-D-(2''-O-acetyl-6''-(E)-p-coumaroyl)-glucopyranoside (2''-acetyl-tiliroside (1), was isolated from the methanolic extract of A. eupatoria stems together with nine known flavonoids. Compounds 4, 7, 8 and 9 all showed a neuroprotective effect on glutamate-induced toxicity in HT22 cells.  (+info)

1beta-Hydroxy-2-oxopomolic acid isolated from Agrimonia pilosa extract inhibits adipogenesis in 3T3-L1 cells. (3/5)

In order to determine anti-adipogenic effect, this study investigated 1beta-hydroxy-2-oxopomolic acid (HOA) isolated from Agrimonia pilosa inhibits adipocyte differentiation and expression of adipogenic marker genes, such as peroxisome proliferator activated receptor gamma (PPARgamma), CCAAT-enhancer-binding protein alpha (C/EBPalpha), glucose transporter 4 (GLUT4), adiponectin, adipocyte fatty acid-binding protein 2 (aP2), adipocyte determination and differentiation factor 1/sterol regulatory element binding protein 1c (ADD1/SREBP1c), resistin, and fatty acid synthase (Fas) in 3T3-L1 preadipocyte. We demonstrated that HOA induced a significant decrease in lipid accumulation and expression of adipogenic marker genes in a dose-dependent manner. In addition, HOA reduced the transcripitional activity of PPARgamma induced by troglitazone, a potent diabetes agent; it also suppressed expression of PPARgamma and C/EBPalpha protein levels. Our data suggest that HOA isolated from Agrimonia pilosa inhibits adipocyte differentiation through downregulation of various adipocytokines by blocking PPARgamma and C/EBPalpha expression.  (+info)

Estrogen-like activity of aqueous extract from Agrimonia pilosa Ledeb. in MCF-7 cells. (4/5)

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Experimental study on inhibition of S180 tumor cells by Agrimonia pilosa extract. (5/5)

We evaluated the in vitro and in vivo inhibition of S180 tumor cell growth of the water extract of Agrimonia pilosa. Inhibitory effect of Agrimonia pilosa on in vitro-cultured S180 cell proliferation was measured by MTT assay; mice model of transplanted tumor was established, after 8 days of continuous administration, the tumors were removed, weighed, and compared with the control group, and the in vivo anti-tumor effect of Agrimonia pilosa on mouse sarcoma S180 was compared using inhibition rate. The in vitro anti-tumor experiment indicated that the inhibition rate gradually increases with the increase of extract concentration and the extension of time, with IC50 175.64, 90.59, and 71.74 microg/ml at 24 h, 4 h, and 72 h respectively. In this study, the in vitro MTT assay was used to determine the inhibitory effect of Agrimony pilosa water extract on S180 tumor cells, the method is simple, reliable, and practical; mice model of in vivo transplanted S180 tumor was established, which allowed direct observation of tumor inhibitory effect, and thus found out that the water extract of Agrimonia pilosa has inhibitory effect on S180 tumor cells.  (+info)