Aggregatibacter actinomycetemcomitans: A species of Gram-negative, facultatively anaerobic spherical or rod-shaped bacteria indigenous to dental surfaces. It is associated with PERIODONTITIS; BACTERIAL ENDOCARDITIS; and ACTINOMYCOSIS.Pasteurellaceae: A family of coccoid to rod-shaped nonsporeforming, gram-negative, nonmotile, facultatively anaerobic bacteria that includes the genera ACTINOBACILLUS; HAEMOPHILUS; MANNHEIMIA; and PASTEURELLA.Pasteurellaceae Infections: Infections with bacteria of the family PASTEURELLACEAE.Actinobacillus Infections: Infections with bacteria of the genus ACTINOBACILLUS.Aggressive Periodontitis: Inflammation and loss of PERIODONTIUM that is characterized by rapid attachment loss and bone destruction in the presence of little local factors such as DENTAL PLAQUE and DENTAL CALCULUS. This highly destructive form of periodontitis often occurs in young people and was called early-onset periodontitis, but this disease also appears in old people.Actinobacillus: A genus of PASTEURELLACEAE described as gram-negative, nonsporeforming, nonmotile, facultative anaerobes. Most members are found both as pathogens and commensal organisms in the respiratory, alimentary, and genital tracts of animals.Exotoxins: Toxins produced, especially by bacterial or fungal cells, and released into the culture medium or environment.Periodontitis: Inflammation and loss of connective tissues supporting or surrounding the teeth. This may involve any part of the PERIODONTIUM. Periodontitis is currently classified by disease progression (CHRONIC PERIODONTITIS; AGGRESSIVE PERIODONTITIS) instead of age of onset. (From 1999 International Workshop for a Classification of Periodontal Diseases and Conditions, American Academy of Periodontology)Chronic Periodontitis: Chronic inflammation and loss of PERIODONTIUM that is associated with the amount of DENTAL PLAQUE or DENTAL CALCULUS present. Chronic periodontitis occurs mostly in adults and was called adult periodontitis, but this disease can appear in young people.Gingiva: Oral tissue surrounding and attached to TEETH.Campylobacter rectus: A species of CAMPYLOBACTER isolated from cases of human PERIODONTITIS. It is a microaerophile, capable of respiring with OXYGEN.Periodontal Pocket: An abnormal extension of a gingival sulcus accompanied by the apical migration of the epithelial attachment and bone resorption.Periodontal Diseases: Pathological processes involving the PERIODONTIUM including the gum (GINGIVA), the alveolar bone (ALVEOLAR PROCESS), the DENTAL CEMENTUM, and the PERIODONTAL LIGAMENT.Dental Plaque: A film that attaches to teeth, often causing DENTAL CARIES and GINGIVITIS. It is composed of MUCINS, secreted from salivary glands, and microorganisms.Gingival Hemorrhage: The flowing of blood from the marginal gingival area, particularly the sulcus, seen in such conditions as GINGIVITIS, marginal PERIODONTITIS, injury, and ASCORBIC ACID DEFICIENCY.Treponema denticola: A species of bacteria in the family SPIROCHAETACEAE, frequently isolated from periodontal pockets (PERIODONTAL POCKET).Fusobacterium nucleatum: A species of gram-negative, anaerobic, rod-shaped bacteria isolated from the gingival margin and sulcus and from infections of the upper respiratory tract and pleural cavity.Prevotella intermedia: A species of gram-negative, anaerobic, rod-shaped bacteria originally classified within the BACTEROIDES genus. This bacterium is a common commensal in the gingival crevice and is often isolated from cases of gingivitis and other purulent lesions related to the mouth.Porphyromonas gingivalis: A species of gram-negative, anaerobic, rod-shaped bacteria originally classified within the BACTEROIDES genus. This bacterium produces a cell-bound, oxygen-sensitive collagenase and is isolated from the human mouth.Dental Devices, Home Care: Devices used in the home by persons to maintain dental and periodontal health. The devices include toothbrushes, dental flosses, water irrigators, gingival stimulators, etc.Bacterial Load: Measurable quantity of bacteria in an object, organism, or organism compartment.Biofilms: Encrustations, formed from microbes (bacteria, algae, fungi, plankton, or protozoa) embedding in extracellular polymers, that adhere to surfaces such as teeth (DENTAL DEPOSITS); PROSTHESES AND IMPLANTS; and catheters. Biofilms are prevented from forming by treating surfaces with DENTIFRICES; DISINFECTANTS; ANTI-INFECTIVE AGENTS; and antifouling agents.Periodontium: The structures surrounding and supporting the tooth. Periodontium includes the gum (GINGIVA), the alveolar bone (ALVEOLAR PROCESS), the DENTAL CEMENTUM, and the PERIODONTAL LIGAMENT.Peri-Implantitis: An inflammatory process with loss of supporting bone in the tissues surrounding functioning DENTAL IMPLANTS.Alveolar Bone Loss: Resorption or wasting of the tooth-supporting bone (ALVEOLAR PROCESS) in the MAXILLA or MANDIBLE.Gingivitis: Inflammation of gum tissue (GINGIVA) without loss of connective tissue.Aggregatibacter: A genus of PASTEURELLACEAE. Members are nonmotile, Gram-negative, facultatively anaerobic rods or coccobacilli. Its members are X factor (HEMIN) independent and variably dependent on V factor (NAD).Bacterial Toxins: Toxic substances formed in or elaborated by bacteria; they are usually proteins with high molecular weight and antigenicity; some are used as antibiotics and some to skin test for the presence of or susceptibility to certain diseases.Streptococcus gordonii: A species of gram-positive, facultatively anaerobic bacteria in the family STREPTOCOCCACEAE. It is a normal inhabitant of the human oral cavity, and causes DENTAL PLAQUE and ENDOCARDITIS. It is being investigated as a vehicle for vaccine delivery.Periodontal Index: A numerical rating scale for classifying the periodontal status of a person or population with a single figure which takes into consideration prevalence as well as severity of the condition. It is based upon probe measurement of periodontal pockets and on gingival tissue status.Dental Plaque Index: An index which scores the degree of dental plaque accumulation.Adhesins, Bacterial: Cell-surface components or appendages of bacteria that facilitate adhesion (BACTERIAL ADHESION) to other cells or to inanimate surfaces. Most fimbriae (FIMBRIAE, BACTERIAL) of gram-negative bacteria function as adhesins, but in many cases it is a minor subunit protein at the tip of the fimbriae that is the actual adhesin. In gram-positive bacteria, a protein or polysaccharide surface layer serves as the specific adhesin. What is sometimes called polymeric adhesin (BIOFILMS) is distinct from protein adhesin.Aggregatibacter segnis: A species of Gram-negative, facultatively anaerobic pleomorphic rod-shaped often filamentous bacteria in the genus of AGGREGATIBACTER found in the oral cavity. It is associated with DENTAL PLAQUE; and BACTERIAL ENDOCARDITIS.Mouth: The oval-shaped oral cavity located at the apex of the digestive tract and consisting of two parts: the vestibule and the oral cavity proper.Gingival Crevicular Fluid: A fluid occurring in minute amounts in the gingival crevice, believed by some authorities to be an inflammatory exudate and by others to cleanse material from the crevice, containing sticky plasma proteins which improve adhesions of the epithelial attachment, have antimicrobial properties, and exert antibody activity. (From Jablonski, Illustrated Dictionary of Dentistry, 1982)Periodontal Attachment Loss: Loss or destruction of periodontal tissue caused by periodontitis or other destructive periodontal diseases or by injury during instrumentation. Attachment refers to the periodontal ligament which attaches to the alveolar bone. It has been hypothesized that treatment of the underlying periodontal disease and the seeding of periodontal ligament cells enable the creating of new attachment.Aggregatibacter aphrophilus: A species of Gram-negative, facultatively anaerobic spherical or rod-shaped bacteria indigenous to oral cavity and pharynx. It is associated with BACTERIAL ENDOCARDITIS; and MENINGITIS.Bacterial Adhesion: Physicochemical property of fimbriated (FIMBRIAE, BACTERIAL) and non-fimbriated bacteria of attaching to cells, tissue, and nonbiological surfaces. It is a factor in bacterial colonization and pathogenicity.Bacteroides: A genus of gram-negative, anaerobic, rod-shaped bacteria. Its organisms are normal inhabitants of the oral, respiratory, intestinal, and urogenital cavities of humans, animals, and insects. Some species may be pathogenic.Bacterial Proteins: Proteins found in any species of bacterium.Gene Expression Regulation, Bacterial: Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.Haemophilus: A genus of PASTEURELLACEAE that consists of several species occurring in animals and humans. Its organisms are described as gram-negative, facultatively anaerobic, coccobacillus or rod-shaped, and nonmotile.Virulence Factors: Those components of an organism that determine its capacity to cause disease but are not required for its viability per se. Two classes have been characterized: TOXINS, BIOLOGICAL and surface adhesion molecules that effect the ability of the microorganism to invade and colonize a host. (From Davis et al., Microbiology, 4th ed. p486)Gram-Negative Bacteria: Bacteria which lose crystal violet stain but are stained pink when treated by Gram's method.Genome, Bacterial: The genetic complement of a BACTERIA as represented in its DNA.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Epithelial Cells: Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.Haemophilus somnus: A species of gram-negative bacteria (currently incertae sedis) causing multisystem disease in CATTLE.Anti-Bacterial Agents: Substances that reduce the growth or reproduction of BACTERIA.Cardiobacterium: A genus of gram-negative, facultatively anaerobic bacteria in the family CARDIOBACTERIACEAE. It is found in the nasal flora of humans and causes ENDOCARDITIS.

Identification of a cytolethal distending toxin gene locus and features of a virulence-associated region in Actinobacillus actinomycetemcomitans. (1/397)

A genetic locus for a cytolethal distending toxin (CDT) was identified in a polymorphic region of the chromosome of Actinobacillus actinomycetemcomitans, a predominant oral pathogen. The locus was comprised of three open reading frames (ORFs) that had significant amino acid sequence similarity and more than 90% sequence identity to the cdtABC genes of some pathogenic Escherichia coli strains and Haemophilus ducreyi, respectively. Sonic extracts from recombinant E. coli, containing the A. actinomycetemcomitans ORFs, caused the distension and killing of Chinese hamster ovary cells characteristic of a CDT. Monoclonal antibodies made reactive with the CdtA, CdtB, and CdtC proteins of H. ducreyi recognized the corresponding gene products from the recombinant strain. CDT-like activities were no longer expressed by the recombinant strain when an OmegaKan-2 interposon was inserted into the cdtA and cdtB genes. Expression of the CDT-like activities in A. actinomycetemcomitans was strain specific. Naturally occurring expression-negative strains had large deletions within the region of the cdt locus. The cdtABC genes were flanked by an ORF (virulence plasmid protein), a partial ORF (integrase), and DNA sequences (bacteriophage integration site) characteristic of virulence-associated regions. These results provide evidence for a functional CDT in a human oral pathogen.  (+info)

Actinobacillus actinomycetemcomitans immunosuppressive protein is a member of the family of cytolethal distending toxins capable of causing a G2 arrest in human T cells. (2/397)

We have previously shown that Actinobacillus actinomycetecomitans produces an immunosuppressive factor (ISF) capable of impairing human lymphocyte function by perturbing cell cycle progression. We now report that ISF is the product of the cdtB gene, one of three genes encoding the family of cytolethal distending toxins (Cdt). The ISF polypeptide exhibits >/=95% identity with Hemophilus ducreyi CdtB protein and +info)

Identification and molecular analysis of rough-colony-specific outer membrane proteins of Actinobacillus actinomycetemcomitans. (3/397)

Actinobacillus actinomycetemcomitans, a gram-negative bacterium isolated from the human mouth, has been implicated in the pathogenesis of early-onset periodontitis. Primary isolates cultured from subgingival plaque exhibit an adherent, rough colony phenotype which spontaneously converts to a nonadherent, smooth phenotype upon in vitro subculture. The rough colony variant produces abundant fimbriae and autoaggregates, while the smooth colony variant is planktonic and produces scant fimbriae. To begin to understand the significance of colony variation in biofilm formation by A. actinomycetemcomitans, outer membrane protein profiles of four isogenic rough and smooth colony variants were compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Two proteins with relative molecular masses of 43 and 20 kDa were expressed by the rough colony variants exclusively. Expression of these proteins was not found to be dependent on growth phase, oxygen tension, or type of complex medium. N-terminal amino acid sequences of these proteins obtained by Edman degradation were compared with sequences from the University of Oklahoma A. actinomycetemcomitans genome database. Two contiguous open reading frames (ORFs) encoding proteins having sequence homology with these proteins were identified. The 43-kDa protein (RcpA [rough colony protein A]) was similar to precursor protein D of the general secretion pathway of gram-negative bacilli, while the 20-kDa protein (RcpB [rough colony protein B]) appeared to be unique. The genes encoding these proteins have been cloned from A. actinomycetemcomitans 283 and sequenced. A BLASTX (gapped BLAST) search of the surrounding ORFs revealed homology with other fimbria-related proteins. These data suggest that the genes encoding the 43-kDa (rcpA) and 20-kDa (rcpB) proteins may be functionally related to each other and to genes that may encode fimbria-associated proteins.  (+info)

A novel NDP-6-deoxyhexosyl-4-ulose reductase in the pathway for the synthesis of thymidine diphosphate-D-fucose. (4/397)

The serotype-specific polysaccharide antigen of Actinobacillus actinomycetemcomitans Y4 (serotype b) consists of D-fucose and L-rhamnose. Thymidine diphosphate (dTDP)-D-fucose is the activated nucleotide sugar form of D-fucose, which has been identified as a constituent of structural polysaccharides in only a few bacteria. In this paper, we show that three dTDP-D-fucose synthetic enzymes are encoded by genes in the gene cluster responsible for the synthesis of serotype b-specific polysaccharide in A. actinomycetemcomitans. The first and second steps of the dTDP-D-fucose synthetic pathway are catalyzed by D-glucose-1-phosphate thymidylyltransferase and dTDP-D-glucose 4,6-dehydratase, which are encoded by rmlA and rmlB in the gene cluster, respectively. These two reactions are common to the well studied dTDP-L-rhamnose synthetic pathway. However, the enzyme catalyzing the last step of the dTDP-D-fucose synthetic pathway has never been reported. We identified the fcd gene encoding a dTDP-4-keto-6-deoxy-D-glucose reductase. After purifying the three enzymes, their enzymatic activities were analyzed by reversed-phase high performance liquid chromatography. In addition, nuclear magnetic resonance analysis and gas-liquid chromatography analysis proved that the fcd gene product converts dTDP-4-keto-6-deoxy-D-glucose to dTDP-D-fucose. Moreover, kinetic analysis of the enzyme indicated that the Km values for dTDP-4-keto-6-deoxy-D-glucose and NADPH are 97.3 and 28.7 microM, respectively, and that the enzyme follows the sequential mechanism. This paper is the first report on the dTDP-D-fucose synthetic pathway and dTDP-4-keto-6-deoxy-D-glucose reductase.  (+info)

Improved multiplex PCR using conserved and species-specific 16S rRNA gene primers for simultaneous detection of Actinobacillus actinomycetemcomitans, Bacteroides forsythus, and Porphyromonas gingivalis. (5/397)

Among putative periodontal pathogens, Actinobacillus actinomycetemcomitans, Bacteroides forsythus, and Porphyromonas gingivalis are most convincingly implicated as etiological agents in periodontitis. Therefore, techniques for detection of those three species would be of value. We previously published a description of a multiplex PCR that detects A. actinomycetemcomitans and P. gingivalis. The present paper presents an improvement on that technique, which now allows more sensitive detection of all three periodontal pathogens. Sensitivity was determined by testing serial dilutions of A. actinomycetemcomitans, B. forsythus, and P. gingivalis cells. Primer specificity was tested against (i) all gene sequences from the GenBank-EMBL database, (ii) six A. actinomycetemcomitans, one B. forsythus, and four P. gingivalis strains, (iii) eight different species of oral bacteria, and (iv) supra- and subgingival plaque samples from 20 healthy subjects and subgingival plaque samples from 10 patients with periodontitis. The multiplex PCR had a detection limit of 10 A. actinomycetemcomitans, 10 P. gingivalis, and 100 B. forsythus cells. Specificity was confirmed by the fact that (i) none of our forward primers were homologous to the 16S rRNA genes of other oral species, (ii) amplicons of predicted size were detected for all A. actinomycetemcomitans, B. forsythus, and P. gingivalis strains tested, and (iii) no amplicons were detected for the eight other bacterial species. A. actinomycetemcomitans, B. forsythus, and P. gingivalis were detected in 6 of 20, 1 of 20, and 11 of 20 of supragingival plaque samples, respectively, and 4 of 20, 7 of 20, and 13 of 20 of subgingival plaque samples, respectively, from periodontally healthy subjects. Among patients with periodontitis, the organisms were detected in 7 of 10, 10 of 10, and 7 of 10 samples, respectively. The simultaneous detection of three periodontal pathogens is an advantage of this technique over conventional PCR assays.  (+info)

Identification of genes coding for exported proteins of Actinobacillus actinomycetemcomitans. (6/397)

Random fusions of genomic DNA fragments to a partial gene encoding a signal sequence-deficient bacterial alkaline phosphatase were utilized to screen for exported proteins of Actinobacillus actinomycetemcomitans in Escherichia coli. Twenty-four PhoA(+) clones were isolated and sequenced. Membrane localization signals in the form of signal sequences were deduced from most of these sequences. Several of the deduced amino acid sequences were found to be homologous to known exported or membrane-associated proteins. The complete genes corresponding to two of these sequences were isolated from an A. actinomycetemcomitans lambda phage library. One gene was found to be homologous to the outer membrane lipoprotein LolB. The second gene product had homology with a Haemophilus influenzae protein and was localized to the inner membrane of A. actinomycetemcomitans.  (+info)

Microtubules are associated with intracellular movement and spread of the periodontopathogen Actinobacillus actinomycetemcomitans. (7/397)

Actinobacillus actinomycetemcomitans SUNY 465, the invasion prototype strain, enters epithelial cells by an actin-dependent mechanism, escapes from the host cell vacuole, and spreads intracellularly and to adjacent epithelial cells via intercellular protrusions. Internalized organisms also egress from host cells into the assay medium via protrusions that are associated with just a single epithelial cell. Here we demonstrate that agents which inhibit microtubule polymerization (e.g., colchicine) and those which stabilize polymerized microtubules (e.g., taxol) both increase markedly the number of intracellular A. actinomycetemcomitans organisms. Furthermore, both colchicine and taxol prevented the egression of A. actinomycetemcomitans from host cells into the assay medium. Immunofluorescence microscopy revealed that protrusions that mediate the bacterial spread contain microtubules. A. actinomycetemcomitans SUNY 465 and 652, strains that are both invasive and egressive, interacted specifically with the plus ends (growing ends) of the filaments of microtubule asters in a KB cell extract. By contrast, neither A. actinomycetemcomitans 523, a strain that is invasive but not egressive, nor Haemophilus aphrophilus, a noninvasive oral bacterium with characteristics similar to those of A. actinomycetemcomitans, bound to microtubules. Together these data suggest that microtubules function in the spread and movement of A. actinomycetemcomitans and provide the first evidence that host cell dispersion of an invasive bacterium may involve the usurption of host cell microtubules.  (+info)

Direct selection of IS903 transposon insertions by use of a broad-host-range vector: isolation of catalase-deficient mutants of Actinobacillus actinomycetemcomitans. (8/397)

Transposon mutagenesis in bacteria generally requires efficient delivery of a transposon suicide vector to allow the selection of relatively infrequent transposition events. We have developed an IS903-based transposon mutagenesis system for diverse gram-negative bacteria that is not limited by transfer efficiency. The transposon, IS903phikan, carries a cryptic kan gene, which can be expressed only after successful transposition. This allows the stable introduction of the transposon delivery vector into the host. Generation of insertion mutants is then limited only by the frequency of transposition. IS903phikan was placed on an IncQ plasmid vector with the transposase gene located outside the transposon and expressed from isopropyl-beta-D-thiogalactopyranoside (IPTG)-inducible promoters. After transposase induction, IS903phikan insertion mutants were readily selected in Escherichia coli by their resistance to kanamycin. We used IS903phikan to isolate three catalase-deficient mutants of the periodontal pathogen Actinobacillus actinomycetemcomitans from a library of random insertions. The mutants display increased sensitivity to hydrogen peroxide, and all have IS903phikan insertions within an open reading frame whose predicted product is closely related to other bacterial catalases. Nucleotide sequence analysis of the catalase gene (designated katA) and flanking intergenic regions also revealed several occurrences of an 11-bp sequence that is closely related to the core DNA uptake signal sequence for natural transformation of Haemophilus influenzae. Our results demonstrate the utility of the IS903phikan mutagenesis system for the study of A. actinomycetemcomitans. Because IS903phikan is carried on a mobilizable, broad-host-range IncQ plasmid, this system is potentially useful in a variety of bacterial species.  (+info)

Two previous studies have observed natural transformation in A. actinomycetemcomitans. Tøonjum et al. (30) used a broth-based method and identified two naturally competent strains among five study strains of A. actinomycetemcomitans with transformation frequencies of 1.3 × 10−3 and 6.2 × 10−5. In our present study, we identified a single highly competent A. actinomycetemcomitans strain among 17 strains examined. Although this is an apparently lower prevalence of naturally competent A. actinomycetemcomitans strains than was reported by Tøonjum et al., the transformation frequencies reported in this study were similar.. Sato et al. (23) generated transposon insertional mutants from the A. actinomycetemcomitans strain Y4 by natural transformation. The transformation was performed with broth-cultured exponential-phase A. actinomycetemcomitans cells. However, strain Y4 was not transformable in our study. While the loss of competence is sometimes a consequence of laboratory propagation, it is ...
Patients with localized juvenile periodontitis (LJP) often have high titers of antibody reactive with the serotype-specific immunodominant carbohydrate antigen of Actinobacillus actinomycetemcomitans serotype b. The vast majority of this A. actinomycetemcomitans serotype b-specific antibody is immunoglobulin G2 (IgG2). The present study was undertaken to determine whether the overall total levels of IgG2 in the sera of LJP patients are elevated. LJP patients and nonperiodontitis (NP) controls matched for age, race (black and white), and gender were studied. Additional controls included patients with adult periodontitis (AP) and patients similar in age to LJP patients but with the more-severe, generalized form of early-onset periodontitis (SP). Sera from over 700 periodontally characterized subjects were examined by using radial immunodiffusion to quantitate IgG2 as well as IgG1, -3, and -4, which were included for comparison. Serum IgG2 levels increased with age, and this was most dramatic ...
Periodontitis is mankinds most common chronic inflammatory disease. One severe form of periodontitis is localized aggressive periodontitis (LAP), a condition to which individuals of African origin demonstrate an increased susceptibility. The main causative organism of this disease is Actinobacillus …
Aggregatibacter (Actinobacillus) actinomycetemcomitans is a bacterium mainly associated with aggressive forms of periodontitis. Among its virulence factors, a leukotoxin is suggested to play an important role in the pathogenicity. Periodontal infections with strains producing high levels of the leukotoxin are strongly associated with severe disease. Leukotoxin selectively kills human leukocytes and can disrupt the local defense mechanisms. Previous studies examining the role of the leukotoxin in host-parasite interactions have mainly focused on polymorphonuclear leukocytes (PMNs). In the inflamed periodontium, macrophages play a significant role in the regulation of the inflammatory reactions and the tissue breakdown and remodeling.. Thus, the aim of this dissertation was to investigate death mechanisms of human macrophages exposed to leukotoxin.. Human lymphocytes, PMNs, and monocytes/macrophages isolated from venous blood were exposed to purified leukotoxin or live A. actinomycetemcomitans ...
Aggregatibacter actinomycetemcomitans ATCC ® 700685D-5™ Designation: Genomic DNA from Aggregatibacter actinomycetemcomitans strain HK1651 TypeStrain=False Application:
Aggregatibacter actinomycetemcomitans ATCC ® 700685D-5™ Designation: Genomic DNA from Aggregatibacter actinomycetemcomitans strain HK1651 TypeStrain=False Application:
Structures of the antigenic O-polysaccharides of lipopolysaccharides produced by Actinobacillus actinomycetemcomitans serotypes a, c, d and e
The ability to selectively target specific biofilm species with antimicrobials would enable control over biofilm consortium composition, with medical applications in treatment of infections on mucosal surfaces that are colonized by a mixture of beneficial and pathogenic microorganisms. We functionalized a genetically engineered multimeric protein with both a targeting moiety (biotin) and either a fluorophore or a photosensitizer (SnCe6). Biofilm microcolonies of Aggregatibacter actinomycetemcomitans, a periodontal pathogen, were targeted with the multifunctional dodecamer. Streptavidin was used to couple biotinylated dodecamer to a biotinylated anti-A. actinomycetemcomitans antibody. This modular targeting approach enabled us to increase the loading of photosensitizer onto the cells by a cycle of amplification. Scanning laser confocal microscopy was used to characterize transport of fluorescently tagged dodecamer into the microcolonies and targeting of the cells with biotin-labeled, ...
Aggregatibacter actinomycetemcomitans is a Gram-negative pathogen that is the etiologic agent of localized aggressive periodontitis (LAP) and other systemic inf...
0165] (1) Wilson M, Henderson, B. Virulence factors of A. actinomycetemcomitans revenant to the pathogenesis of inflammatory periodontal diseases. FEMS 1995:17:365-379. [0166] (2) Nowotny A, Behling U H, Hammond B, et al. Release of toxic microvesicles by Actinobacillus actinomycetemcomitans. Infect Immun 1982:37:151-154. [0167] (3) Kaplan J B, Perry M B, MacLean L L, Furgang D, Wilson M E, Fine D H. Structural and genetic analyses of 0 polysaccharide from Actinobacillus actinomycetemcomitans serotype f. Infect Immun 2001:69:5375-5384. [0168] (4) Rosan B, Slots J, Lamont R J, Listgarten M A, Nelson G M. Actinobacillus actinomycetemcomitans fimbriae. Oral Microbiol Immunol 1988:3:58-63. [0169] (5) Inouye T, Ohta H, Kokeguchi S, Fukui K, Kato K. Colonial variation and fimbriation of Actinobacillus actinomycetemcomitans. FEMS microbiology letters 1990: 57: 13-17. [0170] (6) Kaplan J B, Schreiner H C, Furgang D, Fine D H. Population structure and genetic diversity of Actinobacillus ...
TY - JOUR. T1 - Infection by Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans, and antibody responses at different ages in humans.. AU - Nakagawa, S.. AU - Machida, Y.. AU - Nakagawa, Taneaki. AU - Fujii, H.. AU - Yamada, S.. AU - Takazoe, I.. AU - Okuda, K.. PY - 1994/1. Y1 - 1994/1. N2 - This study examined the serum IgG and IgM responses against Porphyromonas gingivalis and 3 serotypes of Actinobacillus actinomycetemcomitans, and the correlations of these responses with age and homologous infection. A total of 90 individuals were included in this study: 40 subjects with gingivitis, 40 periodontally healthy subjects, and 10 adult periodontitis subjects. The subjects in the gingivitis and periodontally healthy groups were divided into 4 stages based on their physiological age: early childhood, school age, puberty, and adult. In the gingivitis group, there was a positive correlation between increase in age and increase in serum IgG antibody levels against P. gingivalis until ...
Aggregatibacter actinomycetemcomitans is a Gram-negative organism, strongly associated with aggressive forms of periodontitis. An important virulence property of A. actinomycetemcomitans is its ability to form tenacious biofilms that can attach to abiotic as well as biotic surfaces. The histone-like (H-NS) family of nucleoid-structuring proteins act as transcriptional silencers in many Gram-negative bacteria. To evaluate the role of H-NS in A. actinomycetemcomitans, hns mutant derivatives of serotype a strain D7S were generated. Characteristics of the hns mutant phenotype included shorter and fewer pili, and substantially lower monospecies biofilm formation relative to the wild type. Furthermore, the D7S hns mutant exhibited significantly reduced growth within a seven-species oral biofilm model. However, no apparent difference was observed regarding the numbers and proportions of the remaining six species regardless of being co-cultivated with D7S hnsor its parental strain. Proteomics analysis ...
Many gram-positive and gram-negative bacterial species express LuxS and secrete and respond in various ways to a signal that is related to autoinducer 2 of V. harveyi (4, 7, 9, 10, 14, 16, 18, 19, 20, 21, 30, 31, 41). However, few of these organisms possess the dedicated two-component circuit that mediates the cell density-dependent response of V. harveyi to AI-2. Our previous studies showed that AI-2 secreted by the oral pathogen A. actinomycetemcomitans was capable of inducing V. harveyi bioluminescence (10) and also influenced aerobic growth of A. actinomycetemcomitans under iron-limiting conditions (9) These studies also suggested that AI-2-mediated regulation of iron uptake and storage genes contributed to optimal growth of A. actinomycetemcomitans under iron limitation. However, the mechanism by which A. actinomycetemcomitans responds to AI-2 is not fully understood. Analysis of the complete genome sequence of A. actinomycetemcomitans HK1651 (26; www.oralgen.lanl.gov ) indicates that this ...
Adolescent; Aggregatibacter actinomycetemcomitans; Bacterial Toxins; Cohort Studies; Cross-Sectional Studies; DNA, Bacterial; Disease Progression; Ghana; Humans; Pasteurellaceae Infections; Periodontal Diseases ...
Aggregatibacter actinomycetemcomitans GroEL Protein Promotes Conversion of Human CD4 T Cells into IFNγ IL10 Producing Tbet Th1 Cells. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
ID C9R3L9_AGGAD Unreviewed; 404 AA. AC C9R3L9; DT 24-NOV-2009, integrated into UniProtKB/TrEMBL. DT 24-NOV-2009, sequence version 1. DT 25-OCT-2017, entry version 39. DE SubName: Full=Molybdopterin molybdenumtransferase {ECO:0000313,EMBL:ACX82405.1}; GN OrderedLocusNames=D11S_1018 {ECO:0000313,EMBL:ACX82405.1}; OS Aggregatibacter actinomycetemcomitans serotype C (strain D11S-1) OS (Actinobacillus actinomycetemcomitans). OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; OC Pasteurellaceae; Aggregatibacter. OX NCBI_TaxID=668336 {ECO:0000313,EMBL:ACX82405.1, ECO:0000313,Proteomes:UP000002569}; RN [1] {ECO:0000313,EMBL:ACX82405.1, ECO:0000313,Proteomes:UP000002569} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=D11S-1 {ECO:0000313,EMBL:ACX82405.1, RC ECO:0000313,Proteomes:UP000002569}; RX PubMed=19820097; DOI=10.1128/JB.01203-09; RA Chen C., Kittichotirat W., Si Y., Bumgarner R.; RT "Genome sequence of Aggregatibacter actinomycetemcomitans serotype c RT strain D11S-1."; ...
Monoclonal antibody against Aggregatibacter actinomycetemcomitans serotype b expressed by for use in Western Blot against gram-negative bacteria
A major etiologic agent of human periodontal disease. This strain was recovered from the subgingival plaque of a patient diagnosed with generalized aggressive periodontitis ...
Background: This study evaluated the effect of oral lactobacilli on the cytotoxicity and cytokine release from peripheral blood mononuclear cells (PBMC) when exposed to Aggregatibacter actinomycetemcomitans subtypes in vitro. The supernatants and cell wall extracts (CWEs) of eight A. actinomyc...
The focus of my research is the identification and characterization of bacterial proteins that are required for tissue colonization, the initiation of any infection. My research interests are concentrated on the membrane proteins and surface structures of the Gram-negative bacterium Aggregatibacter actinomycetemcomitans. A. actinomycetemcomitans is typically associated with Periodontitis, an inflammatory disease of the tissues surrounding and supporting the teeth, which left untreated results in tooth loss. This bacterium is also associated with numerous non-oral diseases including but not restricted to infective endocarditis and atherosclerosis, which suggest that the periodontal pocket is a potential source and reservoir of these diseases. The emphasis of my research is based on two genes and the associated proteins identified in my laboratory. We have identified and characterized a unique collagen adhesin expressed by A. actinomycetemcomitans. The extracellular matrix protein adhesin A (EmaA) ...
A Periodontite Agressiva (PA), que atualmente pertence ao grupo das Periodontites est gios 3 e 4, distingue-se dos demais tipos de doen a periodontal por seu in cio precoce, agrega o familiar dos casos e por afetar pacientes sistemicamente saud veis. Al m disso, pode ser subclassificada em duas formas, localizada (PAL) e generalizada (PAG), em fun o de sua extens o. Muitas vezes, os dep sitos de biofilme bacteriano s o desproporcionais quantidade de destrui o ssea e perda de inser o que o paciente apresenta, independente da subclassifica o. O microrganismo mais relacionado etiopatog nese da doen a o Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans), incluindo os seus principais sorotipos a, b e c, amplamente estudados. Associado a estas condi es, A. actinomycetemcomitans apresenta alguns fatores de virul ncia como uma leucotoxina, principalmente ligada ao sorotipo b - clone JP2 (que altamente leucot xico) e prote nas de membrana externa (OMPs), especialmente Omp29. A resposta de ...
Johns Hopkins Medicine (JHM). December 14, 2016. Investigators at Johns Hopkins report they have new evidence that a bacterium known to cause chronic inflammatory gum infections also triggers the inflammatory "autoimmune" response characteristic of chronic, joint-destroying rheumatoid arthritis (RA). The new findings have important implications for prevention and treatment of RA, say the researchers.. In a report on the work, published in the Dec. 14 edition of the journal Science Translational Medicine, the investigators say the common denominator they identified in periodontal disease (gum disease) and in many people with RA is Aggregatibacter actinomycetemcomitans. An infection with A. actinomycetemcomitans appears to induce the production of citrullinated proteins, which are suspected of activating the immune system and driving the cascade of events leading to RA.. "This is like putting together the last few pieces of a complicated jigsaw puzzle that has been worked on for many years," says ...
Immunological detection of antigens of Aggregatibacter actinomycetemcomitans, P intermedia, P gingivalisusing antibodies (ELISA ...
What causes rheumatoid arthritis?. The search, by scientists around the world, has been exhaustive, and the prey surprisingly elusive - but in breakthrough Johns Hopkins research, scientists Maximilian Konig, M.D., and Felipe Andrade, M.D., Ph.D., believe they have pinpointed one culprit: a kind of bacteria that lives in the mouth and is present in gum disease.. This form of bacteria has an unpronounceable name, Aggregatibacter actinomycetemcomitans, so the scientists who have discovered the terrible and important role it might play in RA just call it by its initials: Aa. "We are very excited about it," says Konig. If their hypothesis is verified by other scientists and in larger studies, the possibilities of preventing RA from developing, and of treating early RA and possibly reversing symptoms, are enormous. This work was presented at the Plenary Session of the American College of Rheumatologys Annual Scientific Meeting in November 2016, and I recently wrote about it for Leap magazine, a ...
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Investigation of the structure of lipid A from Actinobacillus actinomycetemcomitans strain Y 4 and human clinical isolate PO 1021-7
This study evaluated the effect of oral lactobacilli on the cytotoxicity and cytokine release from peripheral blood mononuclear cells (PBMCs) when exposed to Aggregatibacter actinomycetemcomitans subtypes in vitro. The supernatants and cell wall extracts (CWEs) of eight A. actinomycetemcomitans strains, representing different subtypes, and three Lactobacillus strains were used. The PBMCs from six blood donors were exposed to supernatants and CWEs of A. actinomycetemcomitans or Lactobacillus strains alone or combinations and untreated cells as control. The cytotoxicity was determined by trypan blue exclusion method and IL-1β secretion by ELISA. TNF-α, IL-6, and IL-8 secretions were measured using Bioplex Multiplex Immunoassay. Supernatants or CWEs from all bacterial strains showed cytotoxicity and IL-1β secretion and the subtypes of A. actinomycetemcomitans showed generally a significantly higher effect on PBMCs than that of the Lactobacillus strains. Two highly toxic A. actinomycetemcomitans strains
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Sockett and Lambert, 2004. Balos are ubiquitous in nature and have been isolated from diverse terrestrial and aquatic habitats, from biofilms as well as from animal feces
Pathogenic bacteria produce numerous virulence factors, including protein toxins, to enhance their growth and survival within the host. Our lab seeks to understand the mechanisms of bacterial toxin delivery to identify and exploit therapeutic targets. In this project, we have focused on a member of the repeats-in-toxin (RTX) family of proteins, which are secreted by many Gram negative bacteria, including Bordetella pertussis, Escherichia coli, and Aggregatibacter actinomycetemcomitans. The leukotoxin (LtxA) secreted by A. actinomycetemcomitans specifically kills human white blood cells to disrupt the host immune response and therefore plays a key role in bacterial colonization of the host. The cell type specificity of LtxA arises from its reported targeting of the lymphocyte function-associated antigen-1 (LFA-1) integrin, which is only expressed by human white blood cells. In addition, we recently showed that LtxA binds with a strong affinity to cholesterol, and inhibition of this binding to ...
Aggregatibacter actinomycetemcomitans (Aa) expresses a 64-kDa GroEL protein belonging to the heat shock family of proteins. This protein has been shown to influence human host cells, but the apoptotic capacity of the GroEL protein regarding T cells is not yet known. The purpose of this study was to investigate the ability of A. actinomycetemcomitans GroEL (AaGroEL) protein to induce human peripheral blood T-cell apoptosis. Endogenous, purified AaGroEL protein was used as an antigen. In AaGroEL-treated T cells, the data indicated that phosphatidylserine exposure, an early apoptotic event, was dose- and time-dependent. The AaGroEL-treated T cells were also positive for active caspase-3 in a dose-dependent manner. The rate of AaGroEL-induced apoptosis was suppressed by the addition of the general caspase inhibitor Z-VAD-FMK. Furthermore, cleaved caspase-8 bands (40/36 kDa and 23 kDa) were identified in cells responding to AaGroEL. DNA fragmentation was also detected in the AaGroEL-treated T cells. ...
We have observed that autoantibodies reactive with important phospholipid antigens are present in the sera and gingival crevicular fluids of patients with periodontitis. The antibodies are likely induced by commonly occuring plaque bacteria. Phosphorylcholine (PC) is a prevalent antigen found on 30-40% of plaque bacteria, and patients with periodontal attachment loss have significantly higher concentrations of IgG anti-PC than do individuals without attachment loss. Data indicate that these antibodies bind to and opsonize oral microorganisms such as Actinobacillus actinomycetemcomitans (A. actinomycetemcomitans) and further that they react with and opsonize modified low-density lipoproteins (LDL). Anti-β2-glycoprotein 1-dependent anti-cardiolipin (anti-CL), another antibody of interest, is found at elevated levels in 15-20% of patients with generalized periodontitis. In patients with systemic lupus erythemathosis (SLE) or the antiphospholipid syndrome (APS) elevated levels of these antibodies ...
Objectives: To investigate the short-term effects of nonsurgical therapy (scaling and root planing, SRP) on the subgingival microbiota in chronic (CP) and aggressive (AP) perio dontal disease. Method and Materials: Ninety-seven CP and AP subjects underwent full-mouth SRP on 2 consecutive days. AP patients were randomly assigned to either receive systemic metronidazole plus amoxicillin (AP+AB) or were treated mechanically alone (AP). Pathogens were identified with 16S rRNA oligodeoxynucleotide probes and dot-blot hybridization before and at days 2, 3, 4, 7, 10, and 21 of healing. CP subjects were treated by scaling and root planing along with placebo tablets. Results: Initially, AP cell counts were 69.9- (Porphyromonas gingivalis), 10.2- (Aggregatibacter actinomycetemcomitans), 5.7- (Tannerella forsythia), and 3.3-fold (Prevotella intermedia) enhanced compared to CP cell counts. Following SRP, immediate elimination occurred in single individuals of all three treatment groups at day 2. After SRP ...
Accepted name: dTDP-4-dehydro-6-deoxyglucose reductase. Reaction: dTDP-α-D-fucopyranose + NAD(P)+ = dTDP-4-dehydro-6-deoxy-α-D-glucose + NAD(P)H + H+. For diagram of reaction click here.. Glossary: dTDP-4-dehydro-6-deoxy-α-D-glucose = dTDP-6-deoxy-α-D-xylo-hexopyranos-4-ulose = thymidine 5-[3-(6-deoxy-D-xylo-hexopyranosyl-4-ulose) diphosphate]. Other name(s): dTDP-4-keto-6-deoxyglucose reductase; dTDP-D-fucose:NADP+ oxidoreductase; Fcf1; dTDP-6-deoxy-D-xylo-hex-4-ulopyranose reductase. Systematic name: dTDP-α-D-fucopyranose:NAD(P)+ oxidoreductase. Comments: The enzymes from the Gram-negative bacteria Aggregatibacter actinomycetemcomitans and Escherichia coli O52 are involved in activation of fucose for incorporation into capsular polysaccharide O-antigens [1,3]. The enzyme from the Gram-positive bacterium Geobacillus tepidamans is involved in activation of fucose for incorporation into the organisms S-layer [2].The enzyme from Escherichia coli O52 has a higher catalytic efficiency with ...
Occurrence of Actinobacillus actinomycetemcomitans in patients with chronic periodontitis, aggressive periodontitis, healthy subjects and children with gingivitis in two cities of the state of São Paulo, Brazil ...
The researchers performed full-mouth examinations to assess participants periodontal conditions. Saliva and dental plaque samples were collected to evaluate colonization by several of pathogens - P. gingivalis, T. denticola, T. forsythia, and A. actinomycetemcomitans - and to characterize oral microbial diversity.. Compared with the control group, patients with precancerous lesions experienced higher prevalence of bleeding when probed (31.5 percent versus 22.4 percent), higher levels of two pathogens (T. denticola and A. actinomycetemcomitans), and less bacterial diversity in their saliva.. A further analysis, which took into account sociodemographic factors, oral health behaviors, and periodontal assessments, revealed additional predictors of precancerous lesions: elevated colonization of three pathogens (T. forsythia, T. denticola, and A. actinomycetemcomitans), decreased bacterial diversity in dental plaque, and not flossing regularly.. The researchers concluded that the colonization of ...
The present large-scale, general population-based data suggest that distinct species have a different carriage profile, depending on variables such as age group, educational level, smoking history, and periodontal status. In general, the carriage of periodontal pathogens proved to be common, and only 12% of the 1,294 subjects were free of all six target bacteria examined. More than half of the population had T. forsythensis, one-third T. denticola, P. gingivalis or C. rectus, and one-fifth P. intermedia or A. actinomycetemcomitans. Logistic regression models revealed different variables to be associated with distinct species in dentate subjects.. Periodontitis-associated organisms colonize not only subgingival sites, but also supragingival sites (20, 35), and appear in saliva (5, 7, 8, 15, 30, 32). Stimulation by masticating a piece of paraffin looses still-attached microorganisms or clumps of microorganisms from oral biofilms into salivary sediment. According to Umeda et al. (30), whole saliva ...
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The detection of bacterial-specific volatile metabolites may be a valuable tool to predict infection. Here we applied a real-time mass spectrometric technique to investigate differences in volatile metabolic profiles of oral bacteria that cause periodontitis. We coupled a secondary electrospray ionization (SESI) source to a commercial high-resolution mass spectrometer to interrogate the headspace from bacterial cultures and human saliva. We identified 120 potential markers characteristic for periodontal pathogens Aggregatibacter actinomycetemcomitans (n = 13), Porphyromonas gingivalis (n = 70), Tanerella forsythia (n = 30) and Treponema denticola (n = 7) in in vitro cultures. In a second proof-of-principle phase, we found 18 (P. gingivalis, T. forsythia and T. denticola) of the 120 in vitro compounds in the saliva from a periodontitis patient with confirmed infection with P. gingivalis, T. forsythia and T. denticola with enhanced ion intensity compared to two healthy controls. In conclusion, ...
THE EFFECT OF CURCUMA XANTHORRHIZA ETHANOL EXTRACT ON THE VIABILITY OF STREPTOCOCCUS MUTANS AND AGGREGATIBACTER ACTINOMYCETEMCOMITANS (DENTAL BIOFILM RESEARCH: IN VITRO STUDY)
The cytolethal distending toxins (CDTs) are a family of exotoxins produced by a wide range of Gram-negative bacteria. They are known for causing genotoxic stress to the cell, resulting in growth arrest and eventually apoptotic cell death. Nevertheless, there is evidence that CDTs can also perturb the innate immune responses, by regulating inflammatory cytokine production and molecular mediators of bone remodeling in various cell types. These cellular and molecular events may in turn have an effect in enhancing local inflammation in diseases where CDT-producing bacteria are involved, such as Aggregatibacter actinomycetemcomitans, Haemophilus ducreyi, Campylobacter jejuni and Helicobacter hepaticus. One special example is the induction of pathological bone destruction in periodontitis. The opportunistic oral pathogen Aggregatibatcer actinoycemetemcomitans, which is involved in the aggressive form of the disease, can regulate the molecular mechanisms of bone remodeling in a manner that favors bone
Obradović, Davor, et al. "A Cytolethal Distending Toxin Variant from Aggregatibacter actinomycetemcomitans with an Aberrant CdtB That Lacks the Conserved Catalytic Histidine 160." PloS one 11.7 (2016): e0159231.. Hemp, James, et al. "Draft Genome Sequence of Ornatilinea apprima P3M-1, an Anaerobic Member of the Chloroflexi Class Anaerolineae." Genome Announcements 3.6 (2015): e01353-15.. Ward, Lewis M., et al. "Draft Genome Sequence of Leptolinea tardivitalis YMTK-2, a Mesophilic Anaerobe from the Chloroflexi Class Anaerolineae." Genome Announcements 3.6 (2015): e01356-15.. Hemp, James, et al. "Draft Genome Sequence of Levilinea saccharolytica KIBI-1, a Member of the Chloroflexi Class Anaerolineae." Genome Announcements 3.6 (2015): e01357-15.. Hemp, James, et al. "Draft Genome Sequence of Ardenticatena maritima 110S, a Thermophilic Nitrate-and Iron-Reducing Member of the Chloroflexi Class Ardenticatenia." Genome Announcements 3.6 (2015): e01347-15.. Pace, Laura A., et al. "Draft Genome of ...
Materials and Methods: The present study was designed as a prospective stratified randomized controlled clinical trial. Patients were scheduled to receive two implants with different types of abutments in the posterior mandible. Three months after implant placement, titanium and zirconium abutments were connected. Five weeks after abutment connections, the abutments were removed, probing depth measurements were recorded, and gingival biopsy samples were obtained. Abutments and biopsy specimens were analyzed by reverse-transcriptase polymerase chain reaction to compare the DNA copy numbers of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and total bacteria. The surface free energy of the abutments was calculated by sesile water drop before replacement ...
Teeth/gums (dental generel anaesthsia, mineralization disturbances), Infection (Actinobacillus actinomycetemcomitans, oral pathogens, population genetics of bacteria, virulence factors), Infection (antibiotics), Molecular medicine, Odontology, Teeth/gums (deveopmental disturbances of dental hard tissues, mineralization disturbances). ...
Periodontal Disease or Gum Disease is a common condition affecting about 47% of the population and has been present for millennia. Early humans also suffered from this debilitating condition.. There are usually no symptoms like pain despite the fact that the bone and ligaments holding teeth are slowly melting or literally rotting away as part of inflammation.. There are a few known disease causing bacteria that are present in all mouths such as P gingivalis, P Intermedia, and A actinomycetemcomitans. They are responsible for causing Periodontitis in susceptible individuals. Susceptibility to Periodontal Disease is largely genetically determined. In other words, if your parents, grandparents or the family in the ancestral line had Periodontal Disease, you are more likely to get the disease. There are also modifying conditions including habits, emotional stress, medications and diseases which affect Periodontal disease, for example, diet, oral hygiene, smoking, medications such as steroids or ...
開站以來我們陸續推出了MP150和JC-3兩款後級擴大機,始終少了一個前級套件來搭配,在幾個月的時間中我們開發試裝了幾套前級,最終決定先推出JP200C真空管前級做為diyResearch的第一款前級套件。. 選擇JP200C為第一款前級的主要幾個原因,第一:以Jadis JP200架構基礎的JP200C擁有很迷人的聲音特質。第二:我們對於這個電路很熟悉,這幾年來前前後後裝了許多各種不同版本的JP200,累積了豐富的調教經驗。第三:經過長時間的調整試聽之後,此版本的JP200C非常穩定,同時訊噪比優良。以下先貼出幾張量產PCB的裝機完成品照片供大家瞧瞧。. ...
TY - JOUR. T1 - Relationship of periodontal infection to serum antibody levels to periodontopathic bacteria and inflammatory markers in periodontitis patients with coronary heart disease. AU - Yamazaki, K.. AU - Honda, T.. AU - Domon, H.. AU - Okui, T.. AU - Kajita, K.. AU - Amanuma, R.. AU - Kudoh, C.. AU - Takashiba, S.. AU - Kokeguchi, S.. AU - Nishimura, F.. AU - Kodama, M.. AU - Aizawa, Y.. AU - Oda, H.. PY - 2007/9/1. Y1 - 2007/9/1. N2 - Several reports have demonstrated a possible association of periodontal infections with coronary heart disease (CHD) by elevated antibody titre to periodontopathic bacteria in CHD patients compared with non-diseased controls. Although each periodontopathic bacterium may vary in virulence for periodontitis and atherosclerosis, antibody response to multiple bacteria in CHD patients has not been understood fully. Therefore, serum levels of antibody to 12 periodontopathic bacteria together with other atherosclerotic risk markers were compared among 51 patients ...
Background: The aim of this investigation was to quantify periodontal pathogens (Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Campylobacter rectus and Tannerella forsythia) in vascular, blood and subgingival samples. As secondary objective, two molecular bacterial identification methods [nested-polimerase chain reaction (PCR) and quantitative PCR (qPCR)] were compared.. Methods: Seventy consecutive patients provided a vascular lesion, a blood sample, and 36 subgingival samples. Bacterial deoxyribonucleic acid (DNA) was extracted and qPCR was used to determine the prevalence and amounts of the target pathogens in each sample. Nested-PCR was only performed in the samples from vascular lesions. Periodontal examination was performed in 42 patients. U-Mann-Whitney or Chi-squared tests were used to compare microbiological results according to periodontal diagnosis.. Results: All targeted periodontal pathogens (A. actinomycetemcomitans, P. gingivalis, T. forsythia or C. rectus) were ...
As the aetiology of human actinomycoses is always polymicrobial, the antibacterial drugs used for treatment should in principle cover both the causative actinomycetes and all of the concomitant bacteria. This usually requires the administration of drug combinations in which aminopenicillins currently represent the therapeutic basis because they are slightly more active against the pathogenic actinomycetes than is penicillin G and because they are able to inhibit Aggregatibacter (Actinobacillus) actinomycetemcomitans which is usually resistant to narrow-spectrum penicillins. However, the presence of concomitant β-lactamase producers such as Bacteroides fragilis, B. thetaiotaomicron, or Staphylococcus aureus (β-lactamase producing) may impair the therapeutic efficacy of aminopenicillins and that of many other β-lactams so that the combination with a β-lactamase inhibitor is advisable or even necessary.. For cervicofacial actinomycoses, amoxicillin plus clavulanic acid has proved to be the ...
Principal Investigator:KATO Tetsuo, Project Period (FY):1996 - 1998, Research Category:Grant-in-Aid for Scientific Research (C), Section:一般, Research Field:Morphological basic dentistry
Aggregatibacter actinomycetemcomitans • Aim toothpaste • Akers' clasp • Alberta Dental Association and College • Alfred Fones ...
found in the mouth (e.g. Aggregatibacter actinomycetemcomitans). These are a cause of aggressive juvenile periodontitis. ... "Sensitivity of the periodontal pathogen Aggregatibacter actinomycetemcomitans at mildly acidic pH". J Periodontol. 82 (6): 917- ... Other capnophilic pathogens occur in the Gram-negative Aggregatibacter spp. ...
Aggregatibacter actinomycetemcomitans and E. coli. That is, they are toxic to leukocytes as well as many other cell types and ...
Various studies have associated Aggregatibacter actinomycetemcomitans, formerly known as Actinobacillus actinomycetemcomitans, ... As well as Aggregatibacter actinomycetemcomitans being associated with this, the synergism of the disease also accounts for ... High levels of Aggregatibacter (or Actinobacillus) actinomycetemcomitans and, in some populations, Porphyromonas gingivalis. ... This is due to the suppression of serum IgG2 and antibody against Aggregatibacter actinomycetemcomitans found in smokers. ...
Aggregatibacter actinomycetemcomitans is a Gram-negative coccobacillus prevalent in subgingival plaques. Acinetobacter strains ...
Aggregatibacter actinomycetemcomitans has been identified as being of note in periodontal disease. The genus is typically the ...
Aggregatibacter actinomycetemcomitans is a gram negative coccobacillus which is prevalent in subgingival plaques. Acinetobacter ...
Haemophilus paraphrophilus and Haemophilus segnis as Aggregatibacter actinomycetemcomitans gen. nov., comb. nov., ... Aggregatibacter aphrophilus comb. nov. and Aggregatibacter segnis comb. nov., and emended description of Aggregatibacter ... A. actinomycetemcomitans occurs in the human oral microflora, and together with anaerobic or capnophilic organisms (HACEK group ... An analysis of A. actinomycetemcomitans indicated it was monophyletic with Haemophilus aphrophilus and H. segnis, and it was ...
The retainment of viability despite a dam gene knockout is also seen in Salmonella and Aggregatibacter actinomycetemcomitans. ... A knockout of the dam gene in Aggregatibacter actinomycetemcomitans resulted in dysregulated levels of the protein, leukotoxin ... "Inactivation of DNA adenine methyltransferase alters virulence factors in Actinobacillus actinomycetemcomitans". Oral ...
Slots is responsible for discovering the role of Aggregatibacter actinomycetemcomitans (previously Actinobacillus ... "Comparison of Two Selective Media for Actinobacillus actinomycetemcomitans." J Clin Microbiol 1986;24:636-638. Fryman A, ... actinomycetemcomitans) in aggressive periodontitis, previously known as juvenile periodontitis. In 1982, Slots developed ...
... is produced by Aggregatibacter actinomycetemcomitans, a Gram-negative oral bacterium, when it needs to detach and ... Aggregatibacter actinomycetemcomitans. The bacteria secrete Dispersin B to release adherent cells from a mature biofilm colony ... A. actinomycetemcomitans forms asymmetric biofilm lobed colonies that release single cells or small clusters of bacterial cells ... Kaplan JB, Ragunath C, Ramasubbu N, Fine DH (August 2003). "Detachment of Actinobacillus actinomycetemcomitans biofilm cells by ...
Haemophilus haemolyticus Haemophilus parahaemolyticus Aggregatibacter Aggregatibacter actinomycetemcomitans (previously ... Aggregatibacter segnis Aggregatibacter aphrophilus (previously Haemophilus aphrophilus) Aggregatibacter paraphrophilus ( ... However, taxonomic rearrangements have changed the A to Aggregatibacter species and the H to Haemophilus species to reflect the ... HACEK is an abbreviation of the initials of the genera of this group of bacteria: Haemophilus, Aggregatibacter (previously ...
... a type of agar plate medium used in microbiological testing to select for Aggregatibacter actinomycetemcomitans (A.a.). It was ... "Comparison of Two Selective Media for Actinobacillus actinomycetemcomitans." J Clin Microbiol 1986;24:636-638. Slots, J. " ... "Selective medium for isolation of Actinobacillus actinomycetemcomitans." J Clin Microbiol 1982;15:606-609.. ...
Actinobacteria spp Anaerococcus spp Actinomyces naeslundii Aggregatibacter actinomycetemcomitans Atopobium vaginae Bacteroides ...
Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans; the latter functional defects were improved by treating the ...
Medically important CDT producers include: Haemophilus ducreyi (chancroids) Aggregatibacter actinomycetemcomitans ( ...
H+ The enzyme from bacterium Aggregatibacter actinomycetemcomitans participates in the biosynthesis of the serotype c-specific ... diphosphate-6-deoxy-L-lyxo-4-hexulose reductase synthesizing dTDP-6-deoxy-L-talose from Actinobacillus actinomycetemcomitans". ...
Aggregatibacter actinomycetemcomitans Porphyromonas gingivalis Tannerella forsythia (formerly Bacteroides forsythus) Treponema ...
Haemophilus paraphrophilus and Haemophilus segnis as Aggregatibacter actinomycetemcomitans gen. Nov., comb. Nov., ... Nov., Aggregatibacter aphrophilus comb. Nov. And Aggregatibacter segnis comb. Nov., and emended description of Aggregatibacter ... Nov., Aggregatibacter aphrophilus comb. Nov. And Aggregatibacter segnis comb. Nov., and emended description of Aggregatibacter ... Aggregatibacter aphrophilus comb. Nov. And Aggregatibacter segnis comb. Nov., and emended description of Aggregatibacter ...
... (previously Actinobacillus actinomycetemcomitans) is a Gram-negative, facultative ... nov., Aggregatibacter aphrophilus comb. nov. and Aggregatibacter segnis comb. nov., and emended description of Aggregatibacter ... Type strain of Aggregatibacter actinomycetemcomitans at BacDive - the Bacterial Diversity Metadatabase. ... It was reclassified as Actinobacillus actinomycetemcomitans by Topley & Wilson (1929) and as Haemophilus actinomycetemcomitans ...
JA01 is conserved only among A. actinomycetemcomitans. JA02 is present in both A. actinomycetemcomitans and P. multocida. JA 03 ... sequence were identified by bioinformatics analysis in Aggregatibacter actinomycetemcomitans HK1651 called JA01-JA04. The ... "Novel iron-regulated and Fur-regulated small regulatory RNAs in Aggregatibacter actinomycetemcomitans". Molecular Oral ...
Aggregatibacter actinomycetemcomitans. *HACEK. Legionellales. *Legionella pneumophila/Legionella longbeachae *Legionnaires' ...
Aggregatibacter actinomycetemcomitans. *HACEK. Legionellales. *Legionella pneumophila/Legionella longbeachae *Legionnaires' ...
Aggregatibacter actinomycetemcomitans. *HACEK. Legionellales. *Legionella pneumophila/Legionella longbeachae *Legionnaires' ...
Aggregatibacter actinomycetemcomitans. *HACEK. Legionellales. *Legionella pneumophila/Legionella longbeachae *Legionnaires' ...
Medizinisch bedeutsame Cdt-produzierende Bakterien sind Haemophilus ducreyi, Aggregatibacter actinomycetemcomitans, Escherichia ...
Genomic DNA from Aggregatibacter actinomycetemcomitans strain HK1651 TypeStrain=False Application: ... Actinobacillus actinomycetemcomitans Strain Designations Genomic DNA from Aggregatibacter actinomycetemcomitans strain HK1651 [ ... nov., Aggregatibacter aphrophilus comb. nov. and Aggregatibacter segnis comb. nov., and emended description of Aggregatibacter ... Aggregatibacter actinomycetemcomitans (Klinger 1912) Nørskov-Lauritsen and Kilian 2006 ATCC® 700685D-5™ dried At least 5 µg in ...
Aggregatibacter actinomycetemcomitans H-NS promotes biofilm formation and alters protein dynamics of other species within a ... Aggregatibacter actinomycetemcomitans is a Gram-negative organism, strongly associated with aggressive forms of periodontitis. ... Aggregatibacter actinomycetemcomitans, H-NS, oral biofilm, oral microbiology, aggressive periodontitis, proteomics Nationell ... To evaluate the role of H-NS in A. actinomycetemcomitans, hns mutant derivatives of serotype a strain D7S were generated. ...
Aggregatibacter actinomycetemcomitans is a Gram-negative pathogen that is the etiologic agent of localized aggressive ... Cytotoxic mechanisms of Aggregatibacter actinomycetemcomitans leukotoxin Brown, Angela C. Lehigh University, Bethlehem, PA, ... Cytotoxic mechanisms of Aggregatibacter actinomycetemcomitans leukotoxin. Brown, Angela C. / Lehigh University. $279,310. ... Cytotoxic mechanisms of Aggregatibacter actinomycetemcomitans leukotoxin. Brown, Angela C. / Lehigh University. $246,691. ...
Aggregatibacter actinomycetemcomitans / pathogenicity * Aggregatibacter actinomycetemcomitans / physiology* * Bacterial ... Molecular pathogenicity of the oral opportunistic pathogen Actinobacillus actinomycetemcomitans Annu Rev Microbiol. 2003;57:29- ... In addition to these secreted factors, A. actinomycetemcomitans is invasive with an unusual mechanism for entering, and ... The main causative organism of this disease is Actinobacillus actinomycetemcomitans. A member of the Pasteurellaceae, A. ...
The average level of IgG antibodies against A. actinomycetemcomitans in the school age gingivitis group was significantly ... The average level of IgG antibodies against A. actinomycetemcomitans in the school age gingivitis group was significantly ... The average level of IgG antibodies against A. actinomycetemcomitans in the school age gingivitis group was significantly ... The average level of IgG antibodies against A. actinomycetemcomitans in the school age gingivitis group was significantly ...
The most common ones are Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis.. Gum diseases have become rampant ...
Aggregatibacter actinomycetemcomitans, Aggregatibacter aphrophilus, C hominis, Eikenella corrodens, and Kingella kingae), which ...
Aggregatibacter actinomycetemcomitans (previously Actinobacillus actinomycetemcomitans) is a Gram-negative, facultative ... nov., Aggregatibacter aphrophilus comb. nov. and Aggregatibacter segnis comb. nov., and emended description of Aggregatibacter ... Type strain of Aggregatibacter actinomycetemcomitans at BacDive - the Bacterial Diversity Metadatabase. ... It was reclassified as Actinobacillus actinomycetemcomitans by Topley & Wilson (1929) and as Haemophilus actinomycetemcomitans ...
Genome sequence of Aggregatibacter actinomycetemcomitans serotype c strain D11S-1.. Journal. J Bacteriol 191:7378-9 (2009). DOI ... Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; Pasteurellaceae; Aggregatibacter. Data source. GenBank (Assembly ...
A. actinomycetemcomitans produces leukotoxin (LtxA), an important virulence factor that targets white blood cells (WBCs) and ... actinomycetemcomitans. In this study, we further investigated how LtxA might recognize and lyse RBCs. We found that, in ... Aggregatibacter actinomycetemcomitans is an oral pathogen and etiologic agent of localized aggressive periodontitis. The ... Gangliosides Block Aggregatibacter Actinomycetemcomitans Leukotoxin (LtxA)-Mediated Hemolysis Michael S. Forman, Jason B. ...
Aggregatibacter actinomycetemcomitans e Fusobacterium nucleatum são microrganismos gram-negativos presentes nos processos ... Aggregatibacter actinomycetemcomitans. Fusobacterium nucleatum. Biofilme subgengival. Gengivite. Periodontite crônica. Resumo ... Aggregatibacter actinomycetemcomitans. Fusobacterium nucleatum. Chronic periodontitis. Gingivitis. Subgingival biofilm. Resumo ... Aggregatibacter actinomycetemcomitans e Fusobacterium nucleatum são microrganismos gram-negativos presentes nos processos ...
Mismatch repair - Aggregatibacter actinomycetemcomitans D11S-1 [ Pathway menu , Organism menu , Pathway entry , Download KGML ...
... It has been a long-held supposition of many doctors and researchers that dental ... Aggregatibacter actinomycetemcomitans-induced hypercitrullination links periodontal infection to autoimmunity in rheumatoid ... Aggregatibacter actinomycetemcomitans (Aa), is one of a number of organisms contributing to periodontal gum disease that can ...
nov., Aggregatibacter aphrophilus comb. nov. and Aggregatibacter segnis comb. nov., and emended description of Aggregatibacter ... Aggregatibacter actinomycetemcomitans (Aa) is a gram-negative coccobacillus, facultative anaerobe, closely associated with the ... S. C. Kachlany, "Aggregatibacter actinomycetemcomitans leukotoxin: from threat to therapy," Journal of Dental Research, vol. 89 ... C. Y. Wang, H. C. Wang, J. M. Li et al., "Invasive infections of Aggregatibacter (Actinobacillus) Actinomycetemcomitans," ...
tr,X2JQV5,X2JQV5_AGGAC Oxaloacetate decarboxylase alpha subunit,putative OS=Aggregatibacter actinomycetemcomitans HK1651 OX= ... cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Aggregatibacter › ...
... Ihalin, Riikka Umeå University, ... Our aims were to determine the frequency of ChoP display among Aggregatibacter actinomycetemcomitans strains, to clarify which ... Aggregatibacter actinomycetemcomitans, Serotypes, Periodontitis, Phosphorylcholine, Fimbriae, Flp1, C-reactive protein, Serum ... It further seems that ChoP-positivity does not enhance but may reduce A. actinomycetemcomitans susceptibility to serum killing. ...
IntroductionGram-negative Aggregatibacter actinomycetemcomitans is recognized as an important periodontal pathogen. A striking ... IntroductionGram-negative Aggregatibacter actinomycetemcomitans is recognized as an important periodontal pathogen. A striking ... actinomycetemcomitans strain ATCC29523 (serotype a) was used to construct a deletion mutant of serotype-a specific ...
Cytotoxicity and Leukotoxin Neutralization of Essential Oil from Origanum vulgare against Aggregatibacter actinomycetemcomitans ... Editorial Comments to the Special Issue: "Aggregaterbacter actinomycetemcomitans-Gram-Negative Bacterial Pathogen" ...
... the Gram-negative Aggregatibacter actinomycetemcomitans. For readers with an interest in taxonomy, this microbe was originally ... Aggregatibacter actinomycetemcomitans: A Unique Exotoxin-Producing Oral Bacterium by Pedro Valero-Guillén ... Zijnge V, Kieselbach T, & Oscarsson J (2012). Proteomics of protein secretion by Aggregatibacter actinomycetemcomitans. PloS ... The Toxins of A. actinomycetemcomitans. It has been known for quite some time that A. actinomycetemcomitans makes two toxins, ...
Occurrence of Aggregatibacter actinomycetemcomitans serotypes in subgingival plaque from United States subjects. Mol. Oral ... Comment on "Aggregatibacter actinomycetemcomitans-induced hypercitrullination links periodontal infection to autoimmunity in ... Comment on "Aggregatibacter actinomycetemcomitans-induced hypercitrullination links periodontal infection to autoimmunity in ... Comment on "Aggregatibacter actinomycetemcomitans-induced hypercitrullination links periodontal infection to autoimmunity in ...
Among these, Aggregatibacter actinomycetemcomitans (Aa), but not other candidate pathogens, induced hypercitrullination in host ... Aggregatibacter actinomycetemcomitans generates citrullinated autoantigens involved in rheumatoid arthritis through its pore- ... Aggregatibacter actinomycetemcomitans generates citrullinated autoantigens involved in rheumatoid arthritis through its pore- ... Aggregatibacter actinomycetemcomitans-induced hypercitrullination links periodontal infection to autoimmunity in rheumatoid ...
The Aggregatibacter actinomycetemcomitans cytolethal distending toxin (Cdt) is a heterotrimeric AB2 toxin capable of inducing ... The Aggregatibacter actinomycetemcomitans cytolethal distending toxin (Cdt) is a heterotrimeric AB2 toxin capable of inducing ... The Aggregatibacter actinomycetemcomitans cytolethal distending toxin (Cdt) is a heterotrimeric AB2 toxin capable of inducing ... The Aggregatibacter actinomycetemcomitans cytolethal distending toxin active subunit CdtB contains a cholesterol recognition ...
Biofilm microcolonies of Aggregatibacter actinomycetemcomitans, a periodontal pathogen, were targeted with the multifunctional ... Biofilm microcolonies of Aggregatibacter actinomycetemcomitans, a periodontal pathogen, were targeted with the multifunctional ... Targeted delivery of a photosensitizer to Aggregatibacter actinomycetemcomitans biofilm. Antimicrobial Agents and Chemotherapy ... Streptavidin was used to couple biotinylated dodecamer to a biotinylated anti-A. actinomycetemcomitans antibody. This modular ...
Aggregatibacter actinomycetemcomitans (b) derived from ATCC® 29522™*, SKU: 01175PKWIK-STIK™ 2 PackDETAILS ... Aggregatibacter actinomycetemcomitans (b) derived from ATCC® 29522™*, SKU: 01175LLYFO DISK™DETAILS ...
... actinomycetemcomitans. Although both pathogens induced a T helper 1 pattern of cytokine expression, A. actinomycetemcomitans- ... Results: Both P. gingivalis and A. actinomycetemcomitans led to dendritic cell maturation, but the expression of CD80 was ... While 106 bacteria/mL of P. gingivalis or 104 bacteria/mL of A. actinomycetemcomitans induced expression of interleukin-12p40, ... Conclusion: These results demonstrate that A. actinomycetemcomitans is a more potent immunogen than P. gingivalis because, at ...
  • In addition to these secreted factors, A. actinomycetemcomitans is invasive with an unusual mechanism for entering, and traveling within, eukaryotic cells. (nih.gov)
  • Material and Methods: Using different concentrations of P. gingivalis ATCC 33277 and A. actinomycetemcomitans ATCC 33384, we determined the expression of the maturation markers CD80 and CD86 from purified human dendritic cells by flow cytometry. (csic.es)
  • Although both pathogens induced a T helper 1 pattern of cytokine expression, A. actinomycetemcomitans-stimulated dendritic cells expressed interleukin-1β, interleukin-12, interferon-γ, tumor necrosis factor-α and tumor necrosis factor-β at lower bacterial concentrations than P. gingivalis. (csic.es)
  • Para la detección de Aggregatibacter actinomycetemcomitans se procesaron muestras de placa subgingival obtenidas con cono de papel absorbente, conservadas a -20ºC hasta su procesamiento. (bvsalud.org)
  • Samaranayake notes the evidence for the specific involvement of Aggregatibacter actinomycetemcomitans includes: an increased incidence of it found in subgingival plaque obtained from lesional sites, high level of its antibody which tends to fall following successful treatment, its possession of a wide range of potentially pathogenic products and its elimination with concordant disease regression, following treatment with successful periodontal therapy and adjunctive tetracycline . (wikipedia.org)
  • Aggregatibacter actinomycetemcomitans is a gram negative coccobacillus which is prevalent in subgingival plaques. (medhelp.org)
  • Aggregatibacter actinomycetemcomitans is a Gram-negative microorganism that is frequently isolated from patients with periodontal disease [ 1 ]. (alliedacademies.org)
  • Oral lactobacilli reduce cytotoxicity and cytokine release from peripheral blood mononuclear cells exposed to Aggregatibacter actinomycetemcomitans subtypes in vitro. (researchsquare.com)
  • Background: This study evaluated the effect of oral lactobacilli on the cytotoxicity and cytokine release from peripheral blood mononuclear cells (PBMC) when exposed to Aggregatibacter actinomycetemcomitans subtypes in vitro. (researchsquare.com)
  • In this regard, most Cdts, including the Aggregatibacter actinomycetemcomitans Cdt are heterotrimeric holotoxins that function as AB 2 toxins. (frontiersin.org)
  • Neste estudo, as avaliações qualitativa, quantitativa e genotípica de A. actinomycetemcomitans e F. nucleatum isolados de pacientes com gengivite, periodontite crônica e indivíduos sadios foram realizadas. (usp.br)
  • Os resultados obtidos por AP-PCR mostram a heterogeneidade dos isolados de A. actinomycetemcomitans e de F. nucleatum nos diferentes grupos clínicos de pacientes avaliados. (usp.br)
  • Estudio descriptivo transversal, en el cual se analizaron 23 pacientes diabéticos derivados consecutivamente del Policlínico de Especialidades de la Universidad de los Andes . (bvsalud.org)
  • En el grupo de pacientes diabéticos estudiado, las bacterias más prevalentes fueron Treponema denticola y Tannerella forsythia . (bvsalud.org)
  • A. actinomycetemcomitans invasion induced secretion of interleukin (IL)-1β and expression of inflammasome-associated factors, including nucleotide-binding oligomerization domain like receptor protein 3 (NLRP3), adaptor apoptosisassociated speck-like protein containing a caspase recruitment domain (ASC), caspase-1, caspase-4, and cleaved-gasdermin D (GSDMD), in THP-1 cells. (alliedacademies.org)
  • Docosahexaenoic acid (DHA), an omega-3 fatty acid suppressed NLRP3, ASC and caspase-1 expression as well as IL-1β secretion by THP-1 cells in response to A. actinomycetemcomitans invasion. (alliedacademies.org)
  • The present study investigated the effects of highbush blueberry proanthocyanidins (PACs) on the virulence properties of Aggregatibacter actinomycetemcomitans and macrophage-associated inflammatory responses. (biomedcentral.com)