Aggregatibacter actinomycetemcomitans: A species of Gram-negative, facultatively anaerobic spherical or rod-shaped bacteria indigenous to dental surfaces. It is associated with PERIODONTITIS; BACTERIAL ENDOCARDITIS; and ACTINOMYCOSIS.Pasteurellaceae: A family of coccoid to rod-shaped nonsporeforming, gram-negative, nonmotile, facultatively anaerobic bacteria that includes the genera ACTINOBACILLUS; HAEMOPHILUS; MANNHEIMIA; and PASTEURELLA.Pasteurellaceae Infections: Infections with bacteria of the family PASTEURELLACEAE.Actinobacillus Infections: Infections with bacteria of the genus ACTINOBACILLUS.Aggressive Periodontitis: Inflammation and loss of PERIODONTIUM that is characterized by rapid attachment loss and bone destruction in the presence of little local factors such as DENTAL PLAQUE and DENTAL CALCULUS. This highly destructive form of periodontitis often occurs in young people and was called early-onset periodontitis, but this disease also appears in old people.Actinobacillus: A genus of PASTEURELLACEAE described as gram-negative, nonsporeforming, nonmotile, facultative anaerobes. Most members are found both as pathogens and commensal organisms in the respiratory, alimentary, and genital tracts of animals.Exotoxins: Toxins produced, especially by bacterial or fungal cells, and released into the culture medium or environment.Periodontitis: Inflammation and loss of connective tissues supporting or surrounding the teeth. This may involve any part of the PERIODONTIUM. Periodontitis is currently classified by disease progression (CHRONIC PERIODONTITIS; AGGRESSIVE PERIODONTITIS) instead of age of onset. (From 1999 International Workshop for a Classification of Periodontal Diseases and Conditions, American Academy of Periodontology)Chronic Periodontitis: Chronic inflammation and loss of PERIODONTIUM that is associated with the amount of DENTAL PLAQUE or DENTAL CALCULUS present. Chronic periodontitis occurs mostly in adults and was called adult periodontitis, but this disease can appear in young people.Gingiva: Oral tissue surrounding and attached to TEETH.Campylobacter rectus: A species of CAMPYLOBACTER isolated from cases of human PERIODONTITIS. It is a microaerophile, capable of respiring with OXYGEN.Periodontal Pocket: An abnormal extension of a gingival sulcus accompanied by the apical migration of the epithelial attachment and bone resorption.Periodontal Diseases: Pathological processes involving the PERIODONTIUM including the gum (GINGIVA), the alveolar bone (ALVEOLAR PROCESS), the DENTAL CEMENTUM, and the PERIODONTAL LIGAMENT.Dental Plaque: A film that attaches to teeth, often causing DENTAL CARIES and GINGIVITIS. It is composed of MUCINS, secreted from salivary glands, and microorganisms.Gingival Hemorrhage: The flowing of blood from the marginal gingival area, particularly the sulcus, seen in such conditions as GINGIVITIS, marginal PERIODONTITIS, injury, and ASCORBIC ACID DEFICIENCY.Treponema denticola: A species of bacteria in the family SPIROCHAETACEAE, frequently isolated from periodontal pockets (PERIODONTAL POCKET).Fusobacterium nucleatum: A species of gram-negative, anaerobic, rod-shaped bacteria isolated from the gingival margin and sulcus and from infections of the upper respiratory tract and pleural cavity.Prevotella intermedia: A species of gram-negative, anaerobic, rod-shaped bacteria originally classified within the BACTEROIDES genus. This bacterium is a common commensal in the gingival crevice and is often isolated from cases of gingivitis and other purulent lesions related to the mouth.Porphyromonas gingivalis: A species of gram-negative, anaerobic, rod-shaped bacteria originally classified within the BACTEROIDES genus. This bacterium produces a cell-bound, oxygen-sensitive collagenase and is isolated from the human mouth.Dental Devices, Home Care: Devices used in the home by persons to maintain dental and periodontal health. The devices include toothbrushes, dental flosses, water irrigators, gingival stimulators, etc.Bacterial Load: Measurable quantity of bacteria in an object, organism, or organism compartment.Biofilms: Encrustations, formed from microbes (bacteria, algae, fungi, plankton, or protozoa) embedding in extracellular polymers, that adhere to surfaces such as teeth (DENTAL DEPOSITS); PROSTHESES AND IMPLANTS; and catheters. Biofilms are prevented from forming by treating surfaces with DENTIFRICES; DISINFECTANTS; ANTI-INFECTIVE AGENTS; and antifouling agents.Periodontium: The structures surrounding and supporting the tooth. Periodontium includes the gum (GINGIVA), the alveolar bone (ALVEOLAR PROCESS), the DENTAL CEMENTUM, and the PERIODONTAL LIGAMENT.Peri-Implantitis: An inflammatory process with loss of supporting bone in the tissues surrounding functioning DENTAL IMPLANTS.Alveolar Bone Loss: Resorption or wasting of the tooth-supporting bone (ALVEOLAR PROCESS) in the MAXILLA or MANDIBLE.Gingivitis: Inflammation of gum tissue (GINGIVA) without loss of connective tissue.Aggregatibacter: A genus of PASTEURELLACEAE. Members are nonmotile, Gram-negative, facultatively anaerobic rods or coccobacilli. Its members are X factor (HEMIN) independent and variably dependent on V factor (NAD).Bacterial Toxins: Toxic substances formed in or elaborated by bacteria; they are usually proteins with high molecular weight and antigenicity; some are used as antibiotics and some to skin test for the presence of or susceptibility to certain diseases.Streptococcus gordonii: A species of gram-positive, facultatively anaerobic bacteria in the family STREPTOCOCCACEAE. It is a normal inhabitant of the human oral cavity, and causes DENTAL PLAQUE and ENDOCARDITIS. It is being investigated as a vehicle for vaccine delivery.Periodontal Index: A numerical rating scale for classifying the periodontal status of a person or population with a single figure which takes into consideration prevalence as well as severity of the condition. It is based upon probe measurement of periodontal pockets and on gingival tissue status.Dental Plaque Index: An index which scores the degree of dental plaque accumulation.Adhesins, Bacterial: Cell-surface components or appendages of bacteria that facilitate adhesion (BACTERIAL ADHESION) to other cells or to inanimate surfaces. Most fimbriae (FIMBRIAE, BACTERIAL) of gram-negative bacteria function as adhesins, but in many cases it is a minor subunit protein at the tip of the fimbriae that is the actual adhesin. In gram-positive bacteria, a protein or polysaccharide surface layer serves as the specific adhesin. What is sometimes called polymeric adhesin (BIOFILMS) is distinct from protein adhesin.Aggregatibacter segnis: A species of Gram-negative, facultatively anaerobic pleomorphic rod-shaped often filamentous bacteria in the genus of AGGREGATIBACTER found in the oral cavity. It is associated with DENTAL PLAQUE; and BACTERIAL ENDOCARDITIS.Mouth: The oval-shaped oral cavity located at the apex of the digestive tract and consisting of two parts: the vestibule and the oral cavity proper.Gingival Crevicular Fluid: A fluid occurring in minute amounts in the gingival crevice, believed by some authorities to be an inflammatory exudate and by others to cleanse material from the crevice, containing sticky plasma proteins which improve adhesions of the epithelial attachment, have antimicrobial properties, and exert antibody activity. (From Jablonski, Illustrated Dictionary of Dentistry, 1982)Periodontal Attachment Loss: Loss or destruction of periodontal tissue caused by periodontitis or other destructive periodontal diseases or by injury during instrumentation. Attachment refers to the periodontal ligament which attaches to the alveolar bone. It has been hypothesized that treatment of the underlying periodontal disease and the seeding of periodontal ligament cells enable the creating of new attachment.Aggregatibacter aphrophilus: A species of Gram-negative, facultatively anaerobic spherical or rod-shaped bacteria indigenous to oral cavity and pharynx. It is associated with BACTERIAL ENDOCARDITIS; and MENINGITIS.Bacterial Adhesion: Physicochemical property of fimbriated (FIMBRIAE, BACTERIAL) and non-fimbriated bacteria of attaching to cells, tissue, and nonbiological surfaces. It is a factor in bacterial colonization and pathogenicity.Bacteroides: A genus of gram-negative, anaerobic, rod-shaped bacteria. Its organisms are normal inhabitants of the oral, respiratory, intestinal, and urogenital cavities of humans, animals, and insects. Some species may be pathogenic.Bacterial Proteins: Proteins found in any species of bacterium.Gene Expression Regulation, Bacterial: Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.Haemophilus: A genus of PASTEURELLACEAE that consists of several species occurring in animals and humans. Its organisms are described as gram-negative, facultatively anaerobic, coccobacillus or rod-shaped, and nonmotile.Virulence Factors: Those components of an organism that determine its capacity to cause disease but are not required for its viability per se. Two classes have been characterized: TOXINS, BIOLOGICAL and surface adhesion molecules that effect the ability of the microorganism to invade and colonize a host. (From Davis et al., Microbiology, 4th ed. p486)Gram-Negative Bacteria: Bacteria which lose crystal violet stain but are stained pink when treated by Gram's method.Genome, Bacterial: The genetic complement of a BACTERIA as represented in its DNA.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Epithelial Cells: Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.Haemophilus somnus: A species of gram-negative bacteria (currently incertae sedis) causing multisystem disease in CATTLE.Anti-Bacterial Agents: Substances that reduce the growth or reproduction of BACTERIA.Cardiobacterium: A genus of gram-negative, facultatively anaerobic bacteria in the family CARDIOBACTERIACEAE. It is found in the nasal flora of humans and causes ENDOCARDITIS.

Identification of a cytolethal distending toxin gene locus and features of a virulence-associated region in Actinobacillus actinomycetemcomitans. (1/397)

A genetic locus for a cytolethal distending toxin (CDT) was identified in a polymorphic region of the chromosome of Actinobacillus actinomycetemcomitans, a predominant oral pathogen. The locus was comprised of three open reading frames (ORFs) that had significant amino acid sequence similarity and more than 90% sequence identity to the cdtABC genes of some pathogenic Escherichia coli strains and Haemophilus ducreyi, respectively. Sonic extracts from recombinant E. coli, containing the A. actinomycetemcomitans ORFs, caused the distension and killing of Chinese hamster ovary cells characteristic of a CDT. Monoclonal antibodies made reactive with the CdtA, CdtB, and CdtC proteins of H. ducreyi recognized the corresponding gene products from the recombinant strain. CDT-like activities were no longer expressed by the recombinant strain when an OmegaKan-2 interposon was inserted into the cdtA and cdtB genes. Expression of the CDT-like activities in A. actinomycetemcomitans was strain specific. Naturally occurring expression-negative strains had large deletions within the region of the cdt locus. The cdtABC genes were flanked by an ORF (virulence plasmid protein), a partial ORF (integrase), and DNA sequences (bacteriophage integration site) characteristic of virulence-associated regions. These results provide evidence for a functional CDT in a human oral pathogen.  (+info)

Actinobacillus actinomycetemcomitans immunosuppressive protein is a member of the family of cytolethal distending toxins capable of causing a G2 arrest in human T cells. (2/397)

We have previously shown that Actinobacillus actinomycetecomitans produces an immunosuppressive factor (ISF) capable of impairing human lymphocyte function by perturbing cell cycle progression. We now report that ISF is the product of the cdtB gene, one of three genes encoding the family of cytolethal distending toxins (Cdt). The ISF polypeptide exhibits >/=95% identity with Hemophilus ducreyi CdtB protein and +info)

Identification and molecular analysis of rough-colony-specific outer membrane proteins of Actinobacillus actinomycetemcomitans. (3/397)

Actinobacillus actinomycetemcomitans, a gram-negative bacterium isolated from the human mouth, has been implicated in the pathogenesis of early-onset periodontitis. Primary isolates cultured from subgingival plaque exhibit an adherent, rough colony phenotype which spontaneously converts to a nonadherent, smooth phenotype upon in vitro subculture. The rough colony variant produces abundant fimbriae and autoaggregates, while the smooth colony variant is planktonic and produces scant fimbriae. To begin to understand the significance of colony variation in biofilm formation by A. actinomycetemcomitans, outer membrane protein profiles of four isogenic rough and smooth colony variants were compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Two proteins with relative molecular masses of 43 and 20 kDa were expressed by the rough colony variants exclusively. Expression of these proteins was not found to be dependent on growth phase, oxygen tension, or type of complex medium. N-terminal amino acid sequences of these proteins obtained by Edman degradation were compared with sequences from the University of Oklahoma A. actinomycetemcomitans genome database. Two contiguous open reading frames (ORFs) encoding proteins having sequence homology with these proteins were identified. The 43-kDa protein (RcpA [rough colony protein A]) was similar to precursor protein D of the general secretion pathway of gram-negative bacilli, while the 20-kDa protein (RcpB [rough colony protein B]) appeared to be unique. The genes encoding these proteins have been cloned from A. actinomycetemcomitans 283 and sequenced. A BLASTX (gapped BLAST) search of the surrounding ORFs revealed homology with other fimbria-related proteins. These data suggest that the genes encoding the 43-kDa (rcpA) and 20-kDa (rcpB) proteins may be functionally related to each other and to genes that may encode fimbria-associated proteins.  (+info)

A novel NDP-6-deoxyhexosyl-4-ulose reductase in the pathway for the synthesis of thymidine diphosphate-D-fucose. (4/397)

The serotype-specific polysaccharide antigen of Actinobacillus actinomycetemcomitans Y4 (serotype b) consists of D-fucose and L-rhamnose. Thymidine diphosphate (dTDP)-D-fucose is the activated nucleotide sugar form of D-fucose, which has been identified as a constituent of structural polysaccharides in only a few bacteria. In this paper, we show that three dTDP-D-fucose synthetic enzymes are encoded by genes in the gene cluster responsible for the synthesis of serotype b-specific polysaccharide in A. actinomycetemcomitans. The first and second steps of the dTDP-D-fucose synthetic pathway are catalyzed by D-glucose-1-phosphate thymidylyltransferase and dTDP-D-glucose 4,6-dehydratase, which are encoded by rmlA and rmlB in the gene cluster, respectively. These two reactions are common to the well studied dTDP-L-rhamnose synthetic pathway. However, the enzyme catalyzing the last step of the dTDP-D-fucose synthetic pathway has never been reported. We identified the fcd gene encoding a dTDP-4-keto-6-deoxy-D-glucose reductase. After purifying the three enzymes, their enzymatic activities were analyzed by reversed-phase high performance liquid chromatography. In addition, nuclear magnetic resonance analysis and gas-liquid chromatography analysis proved that the fcd gene product converts dTDP-4-keto-6-deoxy-D-glucose to dTDP-D-fucose. Moreover, kinetic analysis of the enzyme indicated that the Km values for dTDP-4-keto-6-deoxy-D-glucose and NADPH are 97.3 and 28.7 microM, respectively, and that the enzyme follows the sequential mechanism. This paper is the first report on the dTDP-D-fucose synthetic pathway and dTDP-4-keto-6-deoxy-D-glucose reductase.  (+info)

Improved multiplex PCR using conserved and species-specific 16S rRNA gene primers for simultaneous detection of Actinobacillus actinomycetemcomitans, Bacteroides forsythus, and Porphyromonas gingivalis. (5/397)

Among putative periodontal pathogens, Actinobacillus actinomycetemcomitans, Bacteroides forsythus, and Porphyromonas gingivalis are most convincingly implicated as etiological agents in periodontitis. Therefore, techniques for detection of those three species would be of value. We previously published a description of a multiplex PCR that detects A. actinomycetemcomitans and P. gingivalis. The present paper presents an improvement on that technique, which now allows more sensitive detection of all three periodontal pathogens. Sensitivity was determined by testing serial dilutions of A. actinomycetemcomitans, B. forsythus, and P. gingivalis cells. Primer specificity was tested against (i) all gene sequences from the GenBank-EMBL database, (ii) six A. actinomycetemcomitans, one B. forsythus, and four P. gingivalis strains, (iii) eight different species of oral bacteria, and (iv) supra- and subgingival plaque samples from 20 healthy subjects and subgingival plaque samples from 10 patients with periodontitis. The multiplex PCR had a detection limit of 10 A. actinomycetemcomitans, 10 P. gingivalis, and 100 B. forsythus cells. Specificity was confirmed by the fact that (i) none of our forward primers were homologous to the 16S rRNA genes of other oral species, (ii) amplicons of predicted size were detected for all A. actinomycetemcomitans, B. forsythus, and P. gingivalis strains tested, and (iii) no amplicons were detected for the eight other bacterial species. A. actinomycetemcomitans, B. forsythus, and P. gingivalis were detected in 6 of 20, 1 of 20, and 11 of 20 of supragingival plaque samples, respectively, and 4 of 20, 7 of 20, and 13 of 20 of subgingival plaque samples, respectively, from periodontally healthy subjects. Among patients with periodontitis, the organisms were detected in 7 of 10, 10 of 10, and 7 of 10 samples, respectively. The simultaneous detection of three periodontal pathogens is an advantage of this technique over conventional PCR assays.  (+info)

Identification of genes coding for exported proteins of Actinobacillus actinomycetemcomitans. (6/397)

Random fusions of genomic DNA fragments to a partial gene encoding a signal sequence-deficient bacterial alkaline phosphatase were utilized to screen for exported proteins of Actinobacillus actinomycetemcomitans in Escherichia coli. Twenty-four PhoA(+) clones were isolated and sequenced. Membrane localization signals in the form of signal sequences were deduced from most of these sequences. Several of the deduced amino acid sequences were found to be homologous to known exported or membrane-associated proteins. The complete genes corresponding to two of these sequences were isolated from an A. actinomycetemcomitans lambda phage library. One gene was found to be homologous to the outer membrane lipoprotein LolB. The second gene product had homology with a Haemophilus influenzae protein and was localized to the inner membrane of A. actinomycetemcomitans.  (+info)

Microtubules are associated with intracellular movement and spread of the periodontopathogen Actinobacillus actinomycetemcomitans. (7/397)

Actinobacillus actinomycetemcomitans SUNY 465, the invasion prototype strain, enters epithelial cells by an actin-dependent mechanism, escapes from the host cell vacuole, and spreads intracellularly and to adjacent epithelial cells via intercellular protrusions. Internalized organisms also egress from host cells into the assay medium via protrusions that are associated with just a single epithelial cell. Here we demonstrate that agents which inhibit microtubule polymerization (e.g., colchicine) and those which stabilize polymerized microtubules (e.g., taxol) both increase markedly the number of intracellular A. actinomycetemcomitans organisms. Furthermore, both colchicine and taxol prevented the egression of A. actinomycetemcomitans from host cells into the assay medium. Immunofluorescence microscopy revealed that protrusions that mediate the bacterial spread contain microtubules. A. actinomycetemcomitans SUNY 465 and 652, strains that are both invasive and egressive, interacted specifically with the plus ends (growing ends) of the filaments of microtubule asters in a KB cell extract. By contrast, neither A. actinomycetemcomitans 523, a strain that is invasive but not egressive, nor Haemophilus aphrophilus, a noninvasive oral bacterium with characteristics similar to those of A. actinomycetemcomitans, bound to microtubules. Together these data suggest that microtubules function in the spread and movement of A. actinomycetemcomitans and provide the first evidence that host cell dispersion of an invasive bacterium may involve the usurption of host cell microtubules.  (+info)

Direct selection of IS903 transposon insertions by use of a broad-host-range vector: isolation of catalase-deficient mutants of Actinobacillus actinomycetemcomitans. (8/397)

Transposon mutagenesis in bacteria generally requires efficient delivery of a transposon suicide vector to allow the selection of relatively infrequent transposition events. We have developed an IS903-based transposon mutagenesis system for diverse gram-negative bacteria that is not limited by transfer efficiency. The transposon, IS903phikan, carries a cryptic kan gene, which can be expressed only after successful transposition. This allows the stable introduction of the transposon delivery vector into the host. Generation of insertion mutants is then limited only by the frequency of transposition. IS903phikan was placed on an IncQ plasmid vector with the transposase gene located outside the transposon and expressed from isopropyl-beta-D-thiogalactopyranoside (IPTG)-inducible promoters. After transposase induction, IS903phikan insertion mutants were readily selected in Escherichia coli by their resistance to kanamycin. We used IS903phikan to isolate three catalase-deficient mutants of the periodontal pathogen Actinobacillus actinomycetemcomitans from a library of random insertions. The mutants display increased sensitivity to hydrogen peroxide, and all have IS903phikan insertions within an open reading frame whose predicted product is closely related to other bacterial catalases. Nucleotide sequence analysis of the catalase gene (designated katA) and flanking intergenic regions also revealed several occurrences of an 11-bp sequence that is closely related to the core DNA uptake signal sequence for natural transformation of Haemophilus influenzae. Our results demonstrate the utility of the IS903phikan mutagenesis system for the study of A. actinomycetemcomitans. Because IS903phikan is carried on a mobilizable, broad-host-range IncQ plasmid, this system is potentially useful in a variety of bacterial species.  (+info)

*Aggregatibacter actinomycetemcomitans

... (previously Actinobacillus actinomycetemcomitans) is a Gram-negative, facultative ... nov., Aggregatibacter aphrophilus comb. nov. and Aggregatibacter segnis comb. nov., and emended description of Aggregatibacter ... Type strain of Aggregatibacter actinomycetemcomitans at BacDive - the Bacterial Diversity Metadatabase. ... It was reclassified as Actinobacillus actinomycetemcomitans by Topley & Wilson (1929) and as Haemophilus actinomycetemcomitans ...

*Index of oral health and dental articles

Aggregatibacter actinomycetemcomitans • Aim toothpaste • Akers' clasp • Alberta Dental Association and College • Alfred Fones ...

*Capnophile

found in the mouth (e.g. Aggregatibacter actinomycetemcomitans). These are a cause of aggressive juvenile periodontitis. ... "Sensitivity of the periodontal pathogen Aggregatibacter actinomycetemcomitans at mildly acidic pH". J Periodontol. 82 (6): 917- ... Other capnophilic pathogens occur in the Gram-negative Aggregatibacter spp. ...

*Epoxygenase

Aggregatibacter actinomycetemcomitans and E. coli. That is, they are toxic to leukocytes as well as many other cell types and ...

*Aggressive periodontitis

Various studies have associated Aggregatibacter actinomycetemcomitans, formerly known as Actinobacillus actinomycetemcomitans, ... As well as Aggregatibacter actinomycetemcomitans being associated with this, the synergism of the disease also accounts for ... High levels of Aggregatibacter (or Actinobacillus) actinomycetemcomitans and, in some populations, Porphyromonas gingivalis. ... This is due to the suppression of serum IgG2 and antibody against Aggregatibacter actinomycetemcomitans found in smokers. ...

*Coccobacillus

Aggregatibacter actinomycetemcomitans is a Gram-negative coccobacillus prevalent in subgingival plaques. Acinetobacter strains ...

*Actinomyces

Aggregatibacter actinomycetemcomitans has been identified as being of note in periodontal disease. The genus is typically the ...

*Aggregatibacter iron-regulated sRNA

JA01 is conserved only among A. actinomycetemcomitans. JA02 is present in both A. actinomycetemcomitans and P. multocida. JA 03 ... sequence were identified by bioinformatics analysis in Aggregatibacter actinomycetemcomitans HK1651 called JA01-JA04. The ... "Novel iron-regulated and Fur-regulated small regulatory RNAs in Aggregatibacter actinomycetemcomitans". Molecular Oral ...

*Bacterial cellular morphologies

Aggregatibacter actinomycetemcomitans is a gram negative coccobacillus which is prevalent in subgingival plaques. Acinetobacter ...

*Aggregatibacter

Haemophilus paraphrophilus and Haemophilus segnis as Aggregatibacter actinomycetemcomitans gen. Nov., comb. Nov., ... Nov., Aggregatibacter aphrophilus comb. Nov. And Aggregatibacter segnis comb. Nov., and emended description of Aggregatibacter ... Nov., Aggregatibacter aphrophilus comb. Nov. And Aggregatibacter segnis comb. Nov., and emended description of Aggregatibacter ... Aggregatibacter aphrophilus comb. Nov. And Aggregatibacter segnis comb. Nov., and emended description of Aggregatibacter ...

*Actinobacillus

Haemophilus paraphrophilus and Haemophilus segnis as Aggregatibacter actinomycetemcomitans gen. nov., comb. nov., ... Aggregatibacter aphrophilus comb. nov. and Aggregatibacter segnis comb. nov., and emended description of Aggregatibacter ... A. actinomycetemcomitans occurs in the human oral microflora, and together with anaerobic or capnophilic organisms (HACEK group ... An analysis of A. actinomycetemcomitans indicated it was monophyletic with Haemophilus aphrophilus and H. segnis, and it was ...

*Dam methylase

The retainment of viability despite a dam gene knockout is also seen in Salmonella and Aggregatibacter actinomycetemcomitans. ... A knockout of the dam gene in Aggregatibacter actinomycetemcomitans resulted in dysregulated levels of the protein, leukotoxin ... "Inactivation of DNA adenine methyltransferase alters virulence factors in Actinobacillus actinomycetemcomitans". Oral ...

*Jørgen Slots

Slots is responsible for discovering the role of Aggregatibacter actinomycetemcomitans (previously Actinobacillus ... "Comparison of Two Selective Media for Actinobacillus actinomycetemcomitans." J Clin Microbiol 1986;24:636-638. Fryman A, ... actinomycetemcomitans) in aggressive periodontitis, previously known as juvenile periodontitis. In 1982, Slots developed ...

*Dispersin B

... is produced by Aggregatibacter actinomycetemcomitans, a Gram-negative oral bacterium, when it needs to detach and ... Aggregatibacter actinomycetemcomitans. The bacteria secrete Dispersin B to release adherent cells from a mature biofilm colony ... A. actinomycetemcomitans forms asymmetric biofilm lobed colonies that release single cells or small clusters of bacterial cells ... Kaplan JB, Ragunath C, Ramasubbu N, Fine DH (August 2003). "Detachment of Actinobacillus actinomycetemcomitans biofilm cells by ...

*Tryptic soy-serum-bacitracin-vancomycin

... a type of agar plate medium used in microbiological testing to select for Aggregatibacter actinomycetemcomitans (A.a.). It was ... "Comparison of Two Selective Media for Actinobacillus actinomycetemcomitans." J Clin Microbiol 1986;24:636-638. Slots, J. " ... "Selective medium for isolation of Actinobacillus actinomycetemcomitans." J Clin Microbiol 1982;15:606-609.. ...

*List of bacterial vaginosis microbiota

Actinobacteria spp Anaerococcus spp Actinomyces naeslundii Aggregatibacter actinomycetemcomitans Atopobium vaginae Bacteroides ...

*Maresin

Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans; the latter functional defects were improved by treating the ...

*Cytolethal distending toxin

Medically important CDT producers include: Haemophilus ducreyi (chancroids) Aggregatibacter actinomycetemcomitans ( ...

*DTDP-6-deoxy-L-talose 4-dehydrogenase (NAD+)

H+ The enzyme from bacterium Aggregatibacter actinomycetemcomitans participates in the biosynthesis of the serotype c-specific ... diphosphate-6-deoxy-L-lyxo-4-hexulose reductase synthesizing dTDP-6-deoxy-L-talose from Actinobacillus actinomycetemcomitans". ...

*Periodontal pathogen

Aggregatibacter actinomycetemcomitans Porphyromonas gingivalis Tannerella forsythia (formerly Bacteroides forsythus) Treponema ...

*HACEK organisms

Haemophilus haemolyticus Haemophilus parahaemolyticus Aggregatibacter Aggregatibacter actinomycetemcomitans (previously ... Aggregatibacter segnis Aggregatibacter aphrophilus (previously Haemophilus aphrophilus) Aggregatibacter paraphrophilus ( ... However, taxonomic rearrangements have changed the A to Aggregatibacter species and the H to Haemophilus species to reflect the ... HACEK is an abbreviation of the initials of the genera of this group of bacteria: Haemophilus, Aggregatibacter (previously ...
The purpose of this study was to test the hypothesis that diabetes aggravates periodontal destruction induced by Aggregatibacter actinomycetemcomitans infection. Thirty-eight diabetic and 33 normal rats were inoculated with A. actinomycetemcomitans and euthanized at baseline and at 4, 5, and 6 weeks after inoculation. Bone loss and the infiltration of polymorphonuclear leukocytes (PMNs) in gingival epithelium were measured in hematoxylin-eosin-stained sections. The induction of tumor necrosis factor alpha (TNF-alpha) was evaluated by immunohistochemistry and of apoptotic cells by a TUNEL (terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling) assay. After A. actinomycetemcomitans infection, the bone loss in diabetic rats was 1.7-fold and the PMN infiltration 1.6-fold higher than in normoglycemic rats (P , 0.05). The induction of TNF-alpha was 1.5-fold higher and of apoptotic cells was up to 3-fold higher in diabetic versus normoglycemic rats (P , 0.05). Treatment with a ...
Aggregatibacter (Actinobacillus) actinomycetemcomitans is a bacterium mainly associated with aggressive forms of periodontitis. Among its virulence factors, a leukotoxin is suggested to play an important role in the pathogenicity. Periodontal infections with strains producing high levels of the leukotoxin are strongly associated with severe disease. Leukotoxin selectively kills human leukocytes and can disrupt the local defense mechanisms. Previous studies examining the role of the leukotoxin in host-parasite interactions have mainly focused on polymorphonuclear leukocytes (PMNs). In the inflamed periodontium, macrophages play a significant role in the regulation of the inflammatory reactions and the tissue breakdown and remodeling.. Thus, the aim of this dissertation was to investigate death mechanisms of human macrophages exposed to leukotoxin.. Human lymphocytes, PMNs, and monocytes/macrophages isolated from venous blood were exposed to purified leukotoxin or live A. actinomycetemcomitans ...
Background Aggregatibacter actinomycetemcomitans is genetically heterogeneous and comprises distinct clonal lineages that may have different virulence potentials. However, limited information of the strain-to-strain genomic variations is available. Methodology/Principal Findings The genome sequences of 11 A. actinomycetemcomitans strains (serotypes a-f) were generated de novo, annotated and combined with three previously sequenced genomes (serotypes a-c) for comparative genomic analysis. Two major groups were identified; serotypes a, d, e, and f, and serotypes b and c. A serotype e strain was found to be distinct from both groups. The size of the pangenome was 3,301 genes, which included 2,034 core genes and 1,267 flexible genes. The number of core genes is estimated to stabilize at 2,060, while the size of the pangenome is estimated to increase by 16 genes with every additional strain sequenced in the future. Within each strain 16.7-29.4% of the genome belonged to the flexible gene pool. Between any
Aggregatibacter actinomycetemcomitans ATCC ® 700685D-5™ Designation: Genomic DNA from Aggregatibacter actinomycetemcomitans strain HK1651 TypeStrain=False Application:
Aggregatibacter actinomycetemcomitans ATCC ® 700685D-5™ Designation: Genomic DNA from Aggregatibacter actinomycetemcomitans strain HK1651 TypeStrain=False Application:
The ability to selectively target specific biofilm species with antimicrobials would enable control over biofilm consortium composition, with medical applications in treatment of infections on mucosal surfaces that are colonized by a mixture of beneficial and pathogenic microorganisms. We functionalized a genetically engineered multimeric protein with both a targeting moiety (biotin) and either a fluorophore or a photosensitizer (SnCe6). Biofilm microcolonies of Aggregatibacter actinomycetemcomitans, a periodontal pathogen, were targeted with the multifunctional dodecamer. Streptavidin was used to couple biotinylated dodecamer to a biotinylated anti-A. actinomycetemcomitans antibody. This modular targeting approach enabled us to increase the loading of photosensitizer onto the cells by a cycle of amplification. Scanning laser confocal microscopy was used to characterize transport of fluorescently tagged dodecamer into the microcolonies and targeting of the cells with biotin-labeled, ...
Aggregatibacter actinomycetemcomitans is a Gram-negative pathogen that is the etiologic agent of localized aggressive periodontitis (LAP) and other systemic inf...
0165] (1) Wilson M, Henderson, B. Virulence factors of A. actinomycetemcomitans revenant to the pathogenesis of inflammatory periodontal diseases. FEMS 1995:17:365-379. [0166] (2) Nowotny A, Behling U H, Hammond B, et al. Release of toxic microvesicles by Actinobacillus actinomycetemcomitans. Infect Immun 1982:37:151-154. [0167] (3) Kaplan J B, Perry M B, MacLean L L, Furgang D, Wilson M E, Fine D H. Structural and genetic analyses of 0 polysaccharide from Actinobacillus actinomycetemcomitans serotype f. Infect Immun 2001:69:5375-5384. [0168] (4) Rosan B, Slots J, Lamont R J, Listgarten M A, Nelson G M. Actinobacillus actinomycetemcomitans fimbriae. Oral Microbiol Immunol 1988:3:58-63. [0169] (5) Inouye T, Ohta H, Kokeguchi S, Fukui K, Kato K. Colonial variation and fimbriation of Actinobacillus actinomycetemcomitans. FEMS microbiology letters 1990: 57: 13-17. [0170] (6) Kaplan J B, Schreiner H C, Furgang D, Fine D H. Population structure and genetic diversity of Actinobacillus ...
Aim We investigated the association between angiographically verified coronary artery disease (CAD) and subgingival Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia and Treponema denticola. Materials and Methods The cross-sectional study population (n = 445) comprised 171 (38.4%) patients with Stable CAD, 158 (35.5%) with acute coronary syndrome (ACS) and 116 (26.1%) with no significant CAD (No CAD). All patients participated in clinical and radiological oral health examinations. Pooled subgingival bacterial samples were analysed by checkerboard DNA-DNA hybridization assays. Results In all study groups, the presence of P. gingivalis, T. forsythia and T. denticola indicated a significant (p ≤ 0.001) linear association with the extent of alveolar bone loss (ABL), but A. actinomycetemcomitans did not (p = 0.074). With a threshold level of bacterial cells 1 × 105 A. actinomycetemcomitans was significantly more prevalent in the Stable CAD group (42.1%) compared ...
Aggregatibacter actinomycetemcomitans is a Gram-negative organism, strongly associated with aggressive forms of periodontitis. An important virulence property of A. actinomycetemcomitans is its ability to form tenacious biofilms that can attach to abiotic as well as biotic surfaces. The histone-like (H-NS) family of nucleoid-structuring proteins act as transcriptional silencers in many Gram-negative bacteria. To evaluate the role of H-NS in A. actinomycetemcomitans, hns mutant derivatives of serotype a strain D7S were generated. Characteristics of the hns mutant phenotype included shorter and fewer pili, and substantially lower monospecies biofilm formation relative to the wild type. Furthermore, the D7S hns mutant exhibited significantly reduced growth within a seven-species oral biofilm model. However, no apparent difference was observed regarding the numbers and proportions of the remaining six species regardless of being co-cultivated with D7S hnsor its parental strain. Proteomics analysis ...
Adolescent; Aggregatibacter actinomycetemcomitans; Bacterial Toxins; Cohort Studies; Cross-Sectional Studies; DNA, Bacterial; Disease Progression; Ghana; Humans; Pasteurellaceae Infections; Periodontal Diseases ...
Aggregatibacter actinomycetemcomitans GroEL Protein Promotes Conversion of Human CD4 T Cells into IFNγ IL10 Producing Tbet Th1 Cells. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
ID C9R3L9_AGGAD Unreviewed; 404 AA. AC C9R3L9; DT 24-NOV-2009, integrated into UniProtKB/TrEMBL. DT 24-NOV-2009, sequence version 1. DT 25-OCT-2017, entry version 39. DE SubName: Full=Molybdopterin molybdenumtransferase {ECO:0000313,EMBL:ACX82405.1}; GN OrderedLocusNames=D11S_1018 {ECO:0000313,EMBL:ACX82405.1}; OS Aggregatibacter actinomycetemcomitans serotype C (strain D11S-1) OS (Actinobacillus actinomycetemcomitans). OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; OC Pasteurellaceae; Aggregatibacter. OX NCBI_TaxID=668336 {ECO:0000313,EMBL:ACX82405.1, ECO:0000313,Proteomes:UP000002569}; RN [1] {ECO:0000313,EMBL:ACX82405.1, ECO:0000313,Proteomes:UP000002569} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=D11S-1 {ECO:0000313,EMBL:ACX82405.1, RC ECO:0000313,Proteomes:UP000002569}; RX PubMed=19820097; DOI=10.1128/JB.01203-09; RA Chen C., Kittichotirat W., Si Y., Bumgarner R.; RT "Genome sequence of Aggregatibacter actinomycetemcomitans serotype c RT strain D11S-1."; ...
Monoclonal antibody against Aggregatibacter actinomycetemcomitans serotype b expressed by for use in Western Blot against gram-negative bacteria
A major etiologic agent of human periodontal disease. This strain was recovered from the subgingival plaque of a patient diagnosed with generalized aggressive periodontitis ...
The focus of my research is the identification and characterization of bacterial proteins that are required for tissue colonization, the initiation of any infection. My research interests are concentrated on the membrane proteins and surface structures of the Gram-negative bacterium Aggregatibacter actinomycetemcomitans. A. actinomycetemcomitans is typically associated with Periodontitis, an inflammatory disease of the tissues surrounding and supporting the teeth, which left untreated results in tooth loss. This bacterium is also associated with numerous non-oral diseases including but not restricted to infective endocarditis and atherosclerosis, which suggest that the periodontal pocket is a potential source and reservoir of these diseases. The emphasis of my research is based on two genes and the associated proteins identified in my laboratory. We have identified and characterized a unique collagen adhesin expressed by A. actinomycetemcomitans. The extracellular matrix protein adhesin A (EmaA) ...
Johns Hopkins Medicine (JHM). December 14, 2016. Investigators at Johns Hopkins report they have new evidence that a bacterium known to cause chronic inflammatory gum infections also triggers the inflammatory "autoimmune" response characteristic of chronic, joint-destroying rheumatoid arthritis (RA). The new findings have important implications for prevention and treatment of RA, say the researchers.. In a report on the work, published in the Dec. 14 edition of the journal Science Translational Medicine, the investigators say the common denominator they identified in periodontal disease (gum disease) and in many people with RA is Aggregatibacter actinomycetemcomitans. An infection with A. actinomycetemcomitans appears to induce the production of citrullinated proteins, which are suspected of activating the immune system and driving the cascade of events leading to RA.. "This is like putting together the last few pieces of a complicated jigsaw puzzle that has been worked on for many years," says ...
Immunological detection of antigens of Aggregatibacter actinomycetemcomitans, P intermedia, P gingivalisusing antibodies (ELISA ...
What causes rheumatoid arthritis?. The search, by scientists around the world, has been exhaustive, and the prey surprisingly elusive - but in breakthrough Johns Hopkins research, scientists Maximilian Konig, M.D., and Felipe Andrade, M.D., Ph.D., believe they have pinpointed one culprit: a kind of bacteria that lives in the mouth and is present in gum disease.. This form of bacteria has an unpronounceable name, Aggregatibacter actinomycetemcomitans, so the scientists who have discovered the terrible and important role it might play in RA just call it by its initials: Aa. "We are very excited about it," says Konig. If their hypothesis is verified by other scientists and in larger studies, the possibilities of preventing RA from developing, and of treating early RA and possibly reversing symptoms, are enormous. This work was presented at the Plenary Session of the American College of Rheumatologys Annual Scientific Meeting in November 2016, and I recently wrote about it for Leap magazine, a ...
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Looking for online definition of Actinobacillus actinomycetemcomitans in the Medical Dictionary? Actinobacillus actinomycetemcomitans explanation free. What is Actinobacillus actinomycetemcomitans? Meaning of Actinobacillus actinomycetemcomitans medical term. What does Actinobacillus actinomycetemcomitans mean?
Sockett and Lambert, 2004. Balos are ubiquitous in nature and have been isolated from diverse terrestrial and aquatic habitats, from biofilms as well as from animal feces
A. actinomycetemcomitans is an important periodontopathogen that has been implicated in juvenile and adult periodontitis, diseases characterized by rapid destruction of the tooth-supporting tissues. This organism possesses a large number of virulence factors which enable it to colonize the oral cavity, invade periodontal tissues, evade host defences, initiate connective tissue destruction and interfere with tissue repair (for recent reviews see [59, 60]).. Most fresh isolates of A. actinomycetemcomitans are fimbriated, and binding of this microorganism to solid surfaces (e.g. hydroxyapatite and saliva-coated hydroxyapatite) may involve fimbriae [61]. The parameters of A. actinomycetemcomitans adhesion to epithelial cells were assayed by use of different cell lines including the KB cell line, derived from a human oral epidermoid carcinoma [62-64]. Adhesion of A. actinomycetemcomitans to epithelial cells involves multiple determinants (fimbriae, OMPs, vesicles, and/or an extracellular amorphous ...
Aggregatibacter actinomycetemcomitans (Aa) expresses a 64-kDa GroEL protein belonging to the heat shock family of proteins. This protein has been shown to influence human host cells, but the apoptotic capacity of the GroEL protein regarding T cells is not yet known. The purpose of this study was to investigate the ability of A. actinomycetemcomitans GroEL (AaGroEL) protein to induce human peripheral blood T-cell apoptosis. Endogenous, purified AaGroEL protein was used as an antigen. In AaGroEL-treated T cells, the data indicated that phosphatidylserine exposure, an early apoptotic event, was dose- and time-dependent. The AaGroEL-treated T cells were also positive for active caspase-3 in a dose-dependent manner. The rate of AaGroEL-induced apoptosis was suppressed by the addition of the general caspase inhibitor Z-VAD-FMK. Furthermore, cleaved caspase-8 bands (40/36 kDa and 23 kDa) were identified in cells responding to AaGroEL. DNA fragmentation was also detected in the AaGroEL-treated T cells. ...
We have observed that autoantibodies reactive with important phospholipid antigens are present in the sera and gingival crevicular fluids of patients with periodontitis. The antibodies are likely induced by commonly occuring plaque bacteria. Phosphorylcholine (PC) is a prevalent antigen found on 30-40% of plaque bacteria, and patients with periodontal attachment loss have significantly higher concentrations of IgG anti-PC than do individuals without attachment loss. Data indicate that these antibodies bind to and opsonize oral microorganisms such as Actinobacillus actinomycetemcomitans (A. actinomycetemcomitans) and further that they react with and opsonize modified low-density lipoproteins (LDL). Anti-β2-glycoprotein 1-dependent anti-cardiolipin (anti-CL), another antibody of interest, is found at elevated levels in 15-20% of patients with generalized periodontitis. In patients with systemic lupus erythemathosis (SLE) or the antiphospholipid syndrome (APS) elevated levels of these antibodies ...
Objectives: To investigate the short-term effects of nonsurgical therapy (scaling and root planing, SRP) on the subgingival microbiota in chronic (CP) and aggressive (AP) perio dontal disease. Method and Materials: Ninety-seven CP and AP subjects underwent full-mouth SRP on 2 consecutive days. AP patients were randomly assigned to either receive systemic metronidazole plus amoxicillin (AP+AB) or were treated mechanically alone (AP). Pathogens were identified with 16S rRNA oligodeoxynucleotide probes and dot-blot hybridization before and at days 2, 3, 4, 7, 10, and 21 of healing. CP subjects were treated by scaling and root planing along with placebo tablets. Results: Initially, AP cell counts were 69.9- (Porphyromonas gingivalis), 10.2- (Aggregatibacter actinomycetemcomitans), 5.7- (Tannerella forsythia), and 3.3-fold (Prevotella intermedia) enhanced compared to CP cell counts. Following SRP, immediate elimination occurred in single individuals of all three treatment groups at day 2. After SRP ...
Protease Activated Receptors (PARs) are G-protein coupled receptors and they play an active role in host defense. PAR1 and PAR2 are the most highly expressed members of the PAR family in gingival epithelial cells (GECs). This thesis is focused on the role of these two receptors. Objective: This study investigated how PAR receptors regulate downstream signaling pathways and subsequent cytokines secretion in response to specific bacteria in gingival epithelial innate immunity. Methods: Human GECs were transfected with small interfering RNA (siRNA) specific for PAR1 or PAR2. The cells were then stimulated with perio-pathogenic Porphyromonas gingivalis (PG), Aggregatibacter actinomycetemcomitans (AA) or non-pathogenic bacteria Streptococcus gordonii (SG). We used quantitative real-time polymerase chain reaction (QRT-PCR) to detect the messenger RNA (mRNA) expression level of the different innate immune markers; in addition, we used Multi-ELISA ARRAY to measure the protein level of select cytokines ...
Aggregatibacter actinomycetemcomitans (Aa) is a Gram-negative commensal of the human gingival crevice and is an opportunistic periodontal pathogen. The gingival...
Accepted name: dTDP-4-dehydro-6-deoxyglucose reductase. Reaction: dTDP-α-D-fucopyranose + NAD(P)+ = dTDP-4-dehydro-6-deoxy-α-D-glucose + NAD(P)H + H+. For diagram of reaction click here.. Glossary: dTDP-4-dehydro-6-deoxy-α-D-glucose = dTDP-6-deoxy-α-D-xylo-hexopyranos-4-ulose = thymidine 5-[3-(6-deoxy-D-xylo-hexopyranosyl-4-ulose) diphosphate]. Other name(s): dTDP-4-keto-6-deoxyglucose reductase; dTDP-D-fucose:NADP+ oxidoreductase; Fcf1; dTDP-6-deoxy-D-xylo-hex-4-ulopyranose reductase. Systematic name: dTDP-α-D-fucopyranose:NAD(P)+ oxidoreductase. Comments: The enzymes from the Gram-negative bacteria Aggregatibacter actinomycetemcomitans and Escherichia coli O52 are involved in activation of fucose for incorporation into capsular polysaccharide O-antigens [1,3]. The enzyme from the Gram-positive bacterium Geobacillus tepidamans is involved in activation of fucose for incorporation into the organisms S-layer [2].The enzyme from Escherichia coli O52 has a higher catalytic efficiency with ...
Occurrence of Actinobacillus actinomycetemcomitans in patients with chronic periodontitis, aggressive periodontitis, healthy subjects and children with gingivitis in two cities of the state of São Paulo, Brazil ...
The researchers performed full-mouth examinations to assess participants periodontal conditions. Saliva and dental plaque samples were collected to evaluate colonization by several of pathogens - P. gingivalis, T. denticola, T. forsythia, and A. actinomycetemcomitans - and to characterize oral microbial diversity.. Compared with the control group, patients with precancerous lesions experienced higher prevalence of bleeding when probed (31.5 percent versus 22.4 percent), higher levels of two pathogens (T. denticola and A. actinomycetemcomitans), and less bacterial diversity in their saliva.. A further analysis, which took into account sociodemographic factors, oral health behaviors, and periodontal assessments, revealed additional predictors of precancerous lesions: elevated colonization of three pathogens (T. forsythia, T. denticola, and A. actinomycetemcomitans), decreased bacterial diversity in dental plaque, and not flossing regularly.. The researchers concluded that the colonization of ...
Lineage: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; Pasteurellaceae; Aggregatibacter; Aggregatibacter ...
IDCore 30 rev B is a highly secured smartcard platform compliant with Javacard 2.2.2, Global Platform 2.1.1 & 2.2 Amendment D standards, designed to operate with Infineon SLE78 chip family. The library implements TDEA, AES, AES-CMAC, SHA1-224-256-384-512, RSA, RSA CRT, ECDSA, ECC CDH, SP800-108 KDF and SP800-90A DRBG ...
The detection of bacterial-specific volatile metabolites may be a valuable tool to predict infection. Here we applied a real-time mass spectrometric technique to investigate differences in volatile metabolic profiles of oral bacteria that cause periodontitis. We coupled a secondary electrospray ionization (SESI) source to a commercial high-resolution mass spectrometer to interrogate the headspace from bacterial cultures and human saliva. We identified 120 potential markers characteristic for periodontal pathogens Aggregatibacter actinomycetemcomitans (n = 13), Porphyromonas gingivalis (n = 70), Tanerella forsythia (n = 30) and Treponema denticola (n = 7) in in vitro cultures. In a second proof-of-principle phase, we found 18 (P. gingivalis, T. forsythia and T. denticola) of the 120 in vitro compounds in the saliva from a periodontitis patient with confirmed infection with P. gingivalis, T. forsythia and T. denticola with enhanced ion intensity compared to two healthy controls. In conclusion, ...
THE EFFECT OF CURCUMA XANTHORRHIZA ETHANOL EXTRACT ON THE VIABILITY OF STREPTOCOCCUS MUTANS AND AGGREGATIBACTER ACTINOMYCETEMCOMITANS (DENTAL BIOFILM RESEARCH: IN VITRO STUDY)
The cytolethal distending toxins (CDTs) are a family of exotoxins produced by a wide range of Gram-negative bacteria. They are known for causing genotoxic stress to the cell, resulting in growth arrest and eventually apoptotic cell death. Nevertheless, there is evidence that CDTs can also perturb the innate immune responses, by regulating inflammatory cytokine production and molecular mediators of bone remodeling in various cell types. These cellular and molecular events may in turn have an effect in enhancing local inflammation in diseases where CDT-producing bacteria are involved, such as Aggregatibacter actinomycetemcomitans, Haemophilus ducreyi, Campylobacter jejuni and Helicobacter hepaticus. One special example is the induction of pathological bone destruction in periodontitis. The opportunistic oral pathogen Aggregatibatcer actinoycemetemcomitans, which is involved in the aggressive form of the disease, can regulate the molecular mechanisms of bone remodeling in a manner that favors bone
Obradović, Davor, et al. "A Cytolethal Distending Toxin Variant from Aggregatibacter actinomycetemcomitans with an Aberrant CdtB That Lacks the Conserved Catalytic Histidine 160." PloS one 11.7 (2016): e0159231.. Hemp, James, et al. "Draft Genome Sequence of Ornatilinea apprima P3M-1, an Anaerobic Member of the Chloroflexi Class Anaerolineae." Genome Announcements 3.6 (2015): e01353-15.. Ward, Lewis M., et al. "Draft Genome Sequence of Leptolinea tardivitalis YMTK-2, a Mesophilic Anaerobe from the Chloroflexi Class Anaerolineae." Genome Announcements 3.6 (2015): e01356-15.. Hemp, James, et al. "Draft Genome Sequence of Levilinea saccharolytica KIBI-1, a Member of the Chloroflexi Class Anaerolineae." Genome Announcements 3.6 (2015): e01357-15.. Hemp, James, et al. "Draft Genome Sequence of Ardenticatena maritima 110S, a Thermophilic Nitrate-and Iron-Reducing Member of the Chloroflexi Class Ardenticatenia." Genome Announcements 3.6 (2015): e01347-15.. Pace, Laura A., et al. "Draft Genome of ...
An actinomycetemcomitans can cause localized juvenile periodontitis and certain types of adult periodontitis. Optimal treatment of periodontal disease caused by this microorganism requires systemic antibiotic therapy in addition to mechanical debride
The main objective of this work was to assess the antimicrobial properties and the dentin-bonding strength of novel magnesium phosphate cements (MPC). Three formulations of MPC, consisting of magnesium oxide and a phosphate salt, NH4H2PO4, NaH2PO4 or a mixture of both, were evaluated. As a result of the setting reaction, MPC transformed into either struvite (MgNH4PO4·6H2O) when NH4H2PO4 was used or an amorphous magnesium sodium phosphate when NaH2PO4 was used. The MPC had appropriate setting times for hard tissue applications, high early compressive strengths and higher strength of bonding to dentin than commercial mineral trioxide aggregate cement. Bacteriological studies were performed with fresh and aged cements against three bacterial strains, Escherichia coli, Pseudomonas aeruginosa (planktonic and in biofilm) and Aggregatibacter actinomycetemcomitans. These bacteria have been associated with infected implants, as well as other frequent hard tissue related infections. Extracts of different ...
Teeth/gums (dental generel anaesthsia, mineralization disturbances), Infection (Actinobacillus actinomycetemcomitans, oral pathogens, population genetics of bacteria, virulence factors), Infection (antibiotics), Molecular medicine, Odontology, Teeth/gums (deveopmental disturbances of dental hard tissues, mineralization disturbances). ...
Periodontal Disease or Gum Disease is a common condition affecting about 47% of the population and has been present for millennia. Early humans also suffered from this debilitating condition.. There are usually no symptoms like pain despite the fact that the bone and ligaments holding teeth are slowly melting or literally rotting away as part of inflammation.. There are a few known disease causing bacteria that are present in all mouths such as P gingivalis, P Intermedia, and A actinomycetemcomitans. They are responsible for causing Periodontitis in susceptible individuals. Susceptibility to Periodontal Disease is largely genetically determined. In other words, if your parents, grandparents or the family in the ancestral line had Periodontal Disease, you are more likely to get the disease. There are also modifying conditions including habits, emotional stress, medications and diseases which affect Periodontal disease, for example, diet, oral hygiene, smoking, medications such as steroids or ...
開站以來我們陸續推出了MP150和JC-3兩款後級擴大機,始終少了一個前級套件來搭配,在幾個月的時間中我們開發試裝了幾套前級,最終決定先推出JP200C真空管前級做為diyResearch的第一款前級套件。. 選擇JP200C為第一款前級的主要幾個原因,第一:以Jadis JP200架構基礎的JP200C擁有很迷人的聲音特質。第二:我們對於這個電路很熟悉,這幾年來前前後後裝了許多各種不同版本的JP200,累積了豐富的調教經驗。第三:經過長時間的調整試聽之後,此版本的JP200C非常穩定,同時訊噪比優良。以下先貼出幾張量產PCB的裝機完成品照片供大家瞧瞧。. ...
Define Juvenile Periodontitis. Juvenile Periodontitis synonyms, Juvenile Periodontitis pronunciation, Juvenile Periodontitis translation, English dictionary definition of Juvenile Periodontitis. n dentistry the inflammation of periodontal tissue n. 1. inflammatory disease of the periodontium. 2. pyorrhea . Noun 1. periodontitis - a disease that...
Background: The aim of this investigation was to quantify periodontal pathogens (Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Campylobacter rectus and Tannerella forsythia) in vascular, blood and subgingival samples. As secondary objective, two molecular bacterial identification methods [nested-polimerase chain reaction (PCR) and quantitative PCR (qPCR)] were compared.. Methods: Seventy consecutive patients provided a vascular lesion, a blood sample, and 36 subgingival samples. Bacterial deoxyribonucleic acid (DNA) was extracted and qPCR was used to determine the prevalence and amounts of the target pathogens in each sample. Nested-PCR was only performed in the samples from vascular lesions. Periodontal examination was performed in 42 patients. U-Mann-Whitney or Chi-squared tests were used to compare microbiological results according to periodontal diagnosis.. Results: All targeted periodontal pathogens (A. actinomycetemcomitans, P. gingivalis, T. forsythia or C. rectus) were ...
is definitely a periodontopathogen that may play a role in cardiovascular diseases. potential role for this Givinostat microorganism in atherosclerotic lesion formation has been suggested and evidence has been provided of a direct link between the presence of specific periodontal pathogens, including in this disease, since oral infection with accelerates early atherosclerosis (19, 37, 41). Rabbits which were experimentally induced with periodontitis showed more extensive accumulations of lipids in their aortas than control nonperiodontitis rabbits (26). Furthermore, intravenous injections of lead to coronary and atherogenesis in pigs (7). In addition, we have isolated viable from human atherosclerotic plaques (34). These results provide evidence that periodontitis and are risk factors for and may contribute to the pathogenesis of atherosclerosis. can Givinostat invade many cell types, including human oral epithelial cells (29, 46, 58), human gingival fibroblasts (2), human coronary artery smooth ...
Actinobacillus definition, any of several spherical or rod-shaped, aerobic, parasitic bacteria of the genus Actinobacillus, certain species of which, as A. mallei, are pathogenic for animals. See more.
Principal Investigator:KATO Tetsuo, Project Period (FY):1996 - 1998, Research Category:Grant-in-Aid for Scientific Research (C), Section:一般, Research Field:Morphological basic dentistry
Perio Horizons Dental Health Page discusses important topics for the dental consumer and the health care provider and offer advice on treatment, prevention, and wellness.
Biohazard level, growth media and temperature, gram stain, industrial applications and more information for Actinobacillus hominis.
welcome to the emaa 2011 Bienvenue à l emaa 2011 welcome to the emaa 2011 Bienvenue à l emaa 2011 Dear Colleague, For its 7th year, the EMAA (European Masters in Aesthetic and Anti-Aging Medicine) will
AIM: To evaluate the effects of photodynamic therapy (PDT) in the nonsurgical treatment of chronic periodontitis. MATERIALS AND METHODS: A randomized, single-blind, controlled, parallel-group clinical trial was performed. Sixty patients were enrolled: 20 healthy controls and 40 patients with periodontitis. The 40 patients were randomized for scaling and root planing (SRP) or SRP + PDT. Periodontal (plaque index, probing depth, clinical recession, clinical attachment level, bleeding on probing and gingival crevicular fluid volume, corresponding to 381 versus 428 critical sites), microbiological (Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Prevotella intermedia and Campylobacter rectus presence, 18 versus 19 samples) and biochemical (interleukin (IL)-1β, IL-6 and tumour necrosis factor (TNF)-α, receptor activator of nuclear factor-kappaB ligand (RANK-L) and osteoprotegerin (OPG) levels, 18 versus 19 samples) parameters were recorded ...
A multifactorial risk pattern of periodontitis has been recognized, where in addition to host and environmental factors, a pathogenic microbiota plays a primary role. The purpose of the current research was to analyze the prevalence of periodontitis-associated microorganisms in patients with aggressive periodontitis and periodontally healthy elders by using molecular-biologic detection methods like eubacterial PCR-amplification of 16S rDNA in combination with dot-blot hybridization. The oligonucleotide probes for the detection of T. forsythensis, P. gingivalis, A. actinomycetemcomitans, C. rectus, F. nucleatum, Fusobacterium spp., P. intermedia, E. corrodens, V. parvula and C. ochracea were designed and evaluated. The PCR products of 42 cultivated target and closely related bacteria were used for the optimization of hybridization conditions. For the epidemiological study, subgingival plaque was sampled from four pockets and one healthy site of 45 aggressive periodontitis patients as well as from ...
The purpose of this study was to characterize the antioxidant content of honey from 10 different floral sources (sage, orange, tupelo, manuka, buckwheat, acacia, fireweed, clover, Hawaiian Christmasberry, and soy) and determine their effects on growth of selected oral pathogens (e.g., Steptococcus mutans, Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans, Fusobacterium nucleatum). Antioxidant capacity of the honeys was determined via the oxygen radical absorbance capacity (ORAC) assay. Specific antioxidant components including phenolics and ascorbate were determined via HPLC analysis. ORAC values ranged from 3 -17 mmol Trolox equivalent/g honey. A linear correlation was observed between total phenolic content and ORAC activity (R2= 0.9352). Antioxidant capacity of the honeys appeared to be due primarily to their phenolic composition as opposed to enzymatic antioxidants and ascorbic acid. Although all 10 honeys were not effective at inhibiting growth of S. mutans, selected components ...
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Pharmacokinetic-pharmacodynamic (PK/PD) integration and modelling were used to predict dosage schedules for florfenicol for two pig pneumonia pathogens, Actinobacillus pleuropneumoniae and Pasteurella multocida. Pharmacokinetic data were pooled for two bioequivalent products, pioneer and generic formulations, administered intramuscularly to pigs at a dose rate of 15 mg/kg. Antibacterial potency was determined in vitro as minimum inhibitory concentration (MIC) and Mutant Prevention Concentration in broth and pig serum, for six isolates of each organism. For both organisms and for both serum and broth MICs, average concentration:MIC ratios over 48 h were similar and exceeded 2.5:1 and times greater than MIC exceeded 35 h. From in vitro time-kill curves, PK/PD modelling established serum breakpoint values for the index AUC24h/MIC for three levels of inhibition of growth, bacteriostasis and 3 and 4log10 reductions in bacterial count; means were 25.7, 40.2 and 47.0 h, respectively, for P. multocida and 24.6,
Periodontal disease is a condition in which the gums and bones surrounding the teeth are inflamed. It is a common cause of oral discomfort and tooth loss, but in recent years has been linked to a variety of systemic problems. Among them is cardiovascular disease, which is the top cause of death in the developed world. There is evidence that periodontal pathogens invade the bloodstream from the gingival pockets and contribute to the progression of disease through a variety of different mechanisms. First, they initiate the systemic inflammatory process by invading and activating vascular endothelial cells to upregulate adhesion molecules and chemokines, which then in turn activate macrophages to take up low density lipoprotein and deposit it on the luminal wall. Atherosclerotic plaque is pro-thrombotic, which increases the chances of forming blood clots and ischemic attacks. Periodontal pathogens also can induce the proliferation of antibodies that can cross-react with self-antigens, resulting in ...
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A genus of very small, nonmotile, nonsporeforming, aerobic, facultatively anaerobic bacteria containing Gram negative rods interspersed with coccal elements. The metabolism of these bacteria is fermentative. They are pathogenic to animals. The…
Shiramaru, S., Asakura, M., Inoue, H., Nagita, A., Matsuhisa, A. and Yamasaki, S. (2012) A Cytolethal Distending Toxin Gene-Based Multiplex PCR Assay for Detection of Campylobacter spp. in Stool Specimens and Comparison with Culture Method. Journal of Veterinary Medical Science, 74, 857-862.
Marker pathogen analyses with micro-IDent®plus supply data on quality and quantity of 11 periodontopathogenic species and their affiliation to so-called "bacterial complexes". The result of this pooled sample provides information on the pathogen load independent from their localization and represents the optimum basis for an individual therapy which is normally a combination of mechanical forms of therapy and adjunctive systemic or local antibiotic administration. This allows you to significantly increase the success rate of the therapy while the relapse rate is dramatically reduced ...
SP 800-38F (December 2012), Recommendation for Block Cipher Modes of Operation: Methods for Key Wrapping describes cryptographic methods that are approved for "key wrapping," i.e., the protection of the confidentiality and integrity of cryptographic keys. In addition to describing existing methods, this publication specifies two new, deterministic authenticated-encryption modes of operation of the Advanced Encryption Standard (AES) algorithm: the AES Key Wrap (KW) mode and the AES Key Wrap With Padding (KWP) mode. An analogous mode with the Triple Data Encryption Algorithm (TDEA) as the underlying block cipher, called TKW, is also specified, to support legacy applications.. ...
The HACEK organisms are a group of fastidious gram-negative bacteria that are an unusual cause of infective endocarditis, which is an inflammation of the heart due to bacterial infection. HACEK is an abbreviation of the initials of the genera of this group of bacteria: Haemophilus, Aggregatibacter (previously Actinobacillus), Cardiobacterium, Eikenella, Kingella. The HACEK organisms are a normal part of the human microbiota, living in the oral-pharyngeal region. The bacteria were originally grouped because they were thought to be a significant cause of infective endocarditis, but recent literature has shown that they are rare and only responsible for 1.4-3% of all cases of this disease. HACEK originally referred to Haemophilus parainfluenzae, Haemophilus aphrophilus, Actinobacillus actinomycetemcomitans, Cardiobacterium hominis, Eikenella corrodens, and Kingella kingae. However, taxonomic rearrangements have changed the A to Aggregatibacter species and the H to Haemophilus species to reflect the ...
Cardiobacterium hominis is a member of the HACEK group (Haemophilus paraphrophilus, Haemophilus parainfluenzae, Aggregatibacter actinomycetemcomitans, Aggregatibacter aphrophilus, C hominis, Eikenella corrodens, and Kingella kingae), which are fastidious, gram-negative, aerobic bacilli that normally reside in the respiratory tract. They have ...
article{176028, author = {Haesebrouck, Freddy and Chiers, Koen and Van Overbeke, Ingrid and Ducatelle, Richard}, issn = {0378-1135}, journal = {VETERINARY MICROBIOLOGY}, language = {eng}, number = {2-4}, pages = {239--249}, title = {Actinobacillus pleuropneumoniae infections in pigs: the role of virulence factors in pathogenesis and protection.}, volume = {58}, year = {1997 ...
Pleuropneumonia infectioasa a porcului (PIP), cauzata de Actinobacillus pleuropneumoniae (App), poate fi intalnita la porcii de toate varstele, inclusiv la mistreti, dar simptomele clinice cele mai frecvente sunt inregistrate la porcii in faza de finisare, in varsta de peste 12 saptamani. In formele clasice de boala animalele manifesta febra si simptome respiratorii, datorate leziunilor caracteristice de pneumonie hemoragico-necrotica sau pleuropneumonie fibrinoasa. Boala poate evolua supra-acut, fara semne clinice, animalele fiind gasite moarte, acut, mai ales in efectivele naive imunologic, cu pierderi prin mortalitate de 15-20%, sau sub-acut, cu mortalitate mai redusa dar variabila. In efectivele infectate endemic boala se cronicizeaza la animalele care au supravietuit, afectand starea lor de bine si producand o scadere a performantelor zootehnice. Porcii pot fi purtatori fara sa dezvolte vreun raspuns imun. Boala genereaza pierderi economice insemnate.. ...
The Genomic tRNA Database is curated by Todd Lowe and Patricia Chan. Please email [email protected] with any questions and comments. To submit corrections, please use our Bug and Request Tracking System ...
Recent advancements in the periodontal research field are consistent with a new model of pathogenesis according to which periodontitis is initiated by a synergistic and dysbiotic microbial community rather than by select periopathogens, such as the
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Furthermore, our group re ported that AMZ shortens the duration of treatment method for aggressive periodontitis. Apart from our re ports, many groups showed A
Formulated to provide outstanding protection against the most common and virulent serotypes 1, 5 and 7. For prevention of pneumonia in pigs 4 weeks of age or older by infection with the prevalent serotypes of Actinobacillus pleuropneumoniae ...
1998632089, 98632089, DE 69832089 T2, DE 69832089T2, DE-T2-69832089, DE1998632089, DE69832089 T2, DE69832089T2, DE98632089, PCT/1998/3820, PCT/JP/1998/003820, PCT/JP/1998/03820, PCT/JP/98/003820, PCT/JP/98/03820, PCT/JP1998/003820, PCT/JP1998/03820, PCT/JP1998003820, PCT/JP199803820, PCT/JP98/003820, PCT/JP98/03820, PCT/JP98003820, PCT/JP9803820 ...
To the editor: Attention has focused recently on a number of slow-growing, fastidious, gram-negative bacteria, all upper respiratory commensal flora, as causes of infective endocarditis (1): Cardiobacterium hominis, Haemophilus aphrophilus, and Actinobacillus actinomycetemcomitans, patients with underlying valvular heart disease presumably develop vegetations on their valves after bacteremia from an upper respiratory site. Due to the unusual growth characteristics of these organisms, isolation is difficult and the diagnosis may be missed. We haave seen a patient with endocarditis caused by Kingella denitrificans, another member of this group.. A 31-year-old man with aortic stenosis and insufficiency was admitted to Columbia-Presbyterian Medical Center ...
Background: Periodontitis is a multifactorial chronic inflammatory disease characterized by destruction of tooth-supporting tissues. Environmental and genetic factors as well as the immune system participate in this process. Recent studies have attempted to elucidate the role of cytokine networks involved in periodontal diseases. Objective: To assess and compare the levels of IL-4, IL-6 and IL-12 in serum samples of patients with generalized aggressive periodontitis (GAgP) and control individuals. Methods: A total of 50 subjects were included in the study of which 25 patients had generalized aggressive periodontitis and 25 were healthy unrelated age and gender matched patients undergoing extraction and surgical crown lengthening (control group). Local blood samples of patients were collected from surgical sites of pocket reduction and from healthy individuals before tooth extraction or crown lengthening from non inflamed sites. The levels of IL-4, IL-6 and IL-12 were determined by an ELISA assay using
Actinobacillus pleuropneumoniae ApaA protein: an antigenic membrane protein from Actinobacillus pleuropnemoniae homology to ABC transporters; amino acid sequence in first source
It is also becoming more commonly recognized that chronic periodontitis may occur simultaneously with both localized and generalized forms of aggressive periodontitis
Hamster Gingival Epithelial Cells from Creative Bioarray are isolated from gingival tissue of pathogen-free laboratory mice. Hamster Gingival Epithelial Cells are grown in a T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarrays Culture Complete Growth Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml and is delivered frozen. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended ...
Actinobacillus suis has been isolated from the lungs of a 9-month-old cat. The bacterium was characterized biochemically as well as genetically, and its sensitivity profile to different antimicrobial agents was established. The role of this isolate in the cats condition is discussed ...
Biomarkers of infection were screened for their possible role as evaluators of antibiotic treatment in an aerosol infection model of porcine pneumonia caused by Actinobacillus pleuropneumoniae (Ap). Following infection of 12 pigs, clinical signs of pneumonia developed within 20 h, whereafter the animals received a single dose of either danofloxacin (2.5 mg/kg) or tiamulin (10 mg/kg). To test the discriminative properties of the biomarkers, the dosage regimens were designed with an expected difference in therapeutic efficacy in favour of danofloxacin. Accordingly, the danofloxacin-treated pigs recovered clinically within 24h after treatment, whereas tiamulin-treated animals remained clinically ill until the end of the study, 48 h after treatment. A similar Picture was seen for the biomarkers of infection. During the infection period, plasma C-reactive protein (CRP), interleukin-6 and haptoglobin increased, whereas plasma zinc, ascorbic acid and alpha-tocopherol decreased. In the ...
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The optimal production of P. haemolytica leukotoxin in the culture supernatant of a fluid medium is dependent on a number of factors. The leukotoxin has to be produced by using a strain that is known for its ability to produce high quantities of leukotoxin, inoculated into the most suitable type of medium at the correct culture density containing the necessary supplements and harvested after a certain growth period. The volume in which it is produced may also have an influence. Two different procedures are described to produce the leukotoxin in 5 to 15-ℓ quantities in RPMI 1640 medium. The first method used to produce leukotoxin is one that has been repeatedly described since the presence of the leukotoxin was first established in 1978. Using this method seven batches of leukotoxin were produced in litre quantities with leukotoxin activity ranging from 23-67 u/mℓ. The seed culture inoculum is prepared in brain heart infusion broth, which is centrifuged before the organisms are inoculated ...
Periodontitis is common, but aggressive periodontitis (AgP) is thought to progress faster than chronic periodontitis (CP). Reviews suggest that tooth loss as a result of periodontitis is in the order of 1.5-9.8%. The aim of this current review was to investigate tooth loss and disease progression in aggressive periodontitis cases. The Medline and Embase databases [read the full story…]. ...
Results: CP was diagnosed in 49 patients, AgP in 20 patients. The periodontal pathogens Aggregregatibacter actinomycetemcomitans (A.a.), Tannerella forsythia, Porphyromonas gingialis and Treponema denticola) were identified with both PADO and MERI: Fusobacterium nucleatum and Prevotella intermedia were only assessed by MERI. With the loboratory real-time PCR-test A.a. was identified. in series 1 more patients ware tested positive on A.a. with MERI (25%) than with PADO (20.6%). In series 2 the real-time PCR (23.2%) showed a lower prevalence for A.a. than PADO (36.4%). Only 10.3% of the patients in series 1 and 17.2% of the patients in series 2 were tested positive on A.a. with both tests. The used tests showed an agreement of k=0.72 for series 1 and k=0.41 for series 2. The test-differences for the rest bacteria-species were smaller or barely present ...
Gum disease is not limited to adults. Periodontitis (inflammation of the supporting tissues of the teeth or also known as the periodontium) can also occur in young people. Periodontal disease can generally be divided into different types including chronic, aggressive and necrotizing periodontal disease; with aggressive periodontitis commonly seen in the younger age group. Continue reading →. ...
Chronic periodontitis and aggressive periodontitis. Their etiopathogenesis, clinical features, risk factors & clinical photographs of patients.
0004] It is commonly known how to produce the RTX-toxin ApxI by culturing Actinobacillus pleuropneumoniae in a culturing medium to which a calcium salt (i.e. a chemical compound, based on an acid, formed by replacing all or part of the hydrogen ions of the acid with one or more calcium ions) is added. In particular, EP 0 453 024 already describes such a method (see "Example 2", paragraph 2 "Purification and characterisation of hemolysin", subparagraph "Methods"). Note that ApxI used to be referred to as "HLY" (see Frey et al. in "J Gen Microbiol. 1993 August; 139(8): 1723-8"). From this EP patent it is known to add a calcium compound (CaCl2) to the medium. Indeed, in Microbiol Pathogenesis 37 (2004) 29-33 it is stated that transcriptional activity of the ApxI operon is enhanced by addition of calcium to the growth medium. This way, high levels of ApxI can be provided for. The medium should support the growth of APP bacteria. It is commonly known how to constitute a medium that supports growth of ...
The complete nucleotide sequence of a 7.7kb mobilisable plasmid (pM3446F), isolated from a florfenicol resistant isolate of Actinobacillus pleuropneumoniae, showed extended similarity to plasmids found in other members of the Pasteurellaceae containing the floR gene as well as replication and mobilisation genes. Mobilisation into other Pasteurellaceae species confirmed that this plasmid can be transferred horizontally ...
MUNOZ, D; QUEZADA, M y RUIZ, A. Serological behaviour study of Actinobacillus pleuropneumoniae (App) in commercial swine herds from the central region of Chile. Arch. med. vet. [online]. 2008, vol.40, n.2, pp.147-153. ISSN 0301-732X. http://dx.doi.org/10.4067/S0301-732X2008000200006.. In Chile, there was only one existing study on App. This study was designed to determine the maternal immunity duration, the age of seroconversion and the apparent and true prevalence in animals from 7 swine commercial herds. 60 samples were taken per herd and divided into 10 serum samples from animals of 4, 6,10,14,18and21 weeks of age, which were analyzed by ELISA®. Out of the 420 samples, 134 were positive with 112 of them belonging to pigs under 10 weeks of age while only 22 were from animals over 10 weeks of age, which seroconverted presumably due to a natural infection. Regarding maternal immunity duration it was found that around the 10th week of age the animals lose their colostrum antibodies. In ...
Background: Mannheimia haemolytica is the primary bacterial pathogen in causing bovine respiratory disease with tremendous annual losses in the cattle industry. The leukotoxin from M. haemolytica is the predominant virulence factor. Several leukotoxin activity assays are available but not standardized regarding sample preparation and cell line. Furthermore, these assays suffer from a high standard error, a prolonged time consumption and often complex sample pretreatments, which is important from the bioprocess engineering point of view. Results: Within this study, an activity assay based on the continuous cell line BL3.1 combined with a commercial available adenosine triphosphate viability assay kit was established. The leukotoxin activity was found to be strongly dependent on the sample preparation. Furthermore, the interfering effect of lipopolysaccharides in the sample could be successfully suppressed by adding polymyxin B. We reached a maximum relative P95 value of 14%, which is more than ...
The investigators demonstrated the effects of such periodontopathic bacteria on HIV-1 replication. They found that P. gingivalis could strongly facilitate HIV-1 reactivation via chromatin modification. The bacteria produced high concentrations of butyric acid, a potent inhibitor of HDACs, and induced acetylation of histones, leading to reactivation of HIV-1 in latently infected cells. These results suggest that periodontal disease could act as a risk-factor for HIV-1 reactivation in latently infected individuals, and might contribute to the systemic dissemination of the virus causing clinical progression of acquired immunodeficiency syndrome (AIDS). The findings emphasize the essential role of maintaining oral hygiene and controlling oral diseases for the prevention of AIDS ...
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Aggregatibacter actinomycetemcomitans (Klinger 1912) Nørskov-LauritsenAggregatibacter actinomycetemcomitans (Klinger 1912) Nørskov-Lauritsen

Genomic DNA from Aggregatibacter actinomycetemcomitans strain HK1651 TypeStrain=False Application: ... Actinobacillus actinomycetemcomitans Strain Designations Genomic DNA from Aggregatibacter actinomycetemcomitans strain HK1651 [ ... nov., Aggregatibacter aphrophilus comb. nov. and Aggregatibacter segnis comb. nov., and emended description of Aggregatibacter ... Aggregatibacter actinomycetemcomitans (Klinger 1912) Nørskov-Lauritsen and Kilian 2006 ATCC® 700685D-5™ dried At least 5 µg in ...
more infohttps://www.atcc.org/en/Products/Nucleic_Acid_Proteins_and_Cell_Extracts/Genomic_DNA_and_RNA/From_Bacteria/700685D-5.aspx

Aggregatibacter actinomycetemcomitans (Klinger 1912) Nørskov-LauritsenAggregatibacter actinomycetemcomitans (Klinger 1912) Nørskov-Lauritsen

Genomic DNA from Aggregatibacter actinomycetemcomitans strain HK1651 TypeStrain=False Application: ... Actinobacillus actinomycetemcomitans Strain Designations Genomic DNA from Aggregatibacter actinomycetemcomitans strain HK1651 [ ... nov., Aggregatibacter aphrophilus comb. nov. and Aggregatibacter segnis comb. nov., and emended description of Aggregatibacter ... Aggregatibacter actinomycetemcomitans (Klinger 1912) Nørskov-Lauritsen and Kilian 2006 ATCC® 700685D-5™ dried At least 5 µg in ...
more infohttps://www.atcc.org/Products/All/700685D-5.aspx

Targeted delivery of a photosensitizer to Aggregatibacter actinomycetemcomitans biofilm  - Zurich Open Repository and ArchiveTargeted delivery of a photosensitizer to Aggregatibacter actinomycetemcomitans biofilm - Zurich Open Repository and Archive

Biofilm microcolonies of Aggregatibacter actinomycetemcomitans, a periodontal pathogen, were targeted with the multifunctional ... Biofilm microcolonies of Aggregatibacter actinomycetemcomitans, a periodontal pathogen, were targeted with the multifunctional ... Targeted delivery of a photosensitizer to Aggregatibacter actinomycetemcomitans biofilm. Antimicrobial Agents and Chemotherapy ... Streptavidin was used to couple biotinylated dodecamer to a biotinylated anti-A. actinomycetemcomitans antibody. This modular ...
more infohttp://www.zora.uzh.ch/id/eprint/42943/

Aggregatibacter actinomycetemcomitans H-NS promotes biofilm formation and alters protein dynamics of other species within a...Aggregatibacter actinomycetemcomitans H-NS promotes biofilm formation and alters protein dynamics of other species within a...

Aggregatibacter actinomycetemcomitans H-NS promotes biofilm formation and alters protein dynamics of other species within a ... Aggregatibacter actinomycetemcomitans is a Gram-negative organism, strongly associated with aggressive forms of periodontitis. ... Aggregatibacter actinomycetemcomitans, H-NS, oral biofilm, oral microbiology, aggressive periodontitis, proteomics Nationell ... To evaluate the role of H-NS in A. actinomycetemcomitans, hns mutant derivatives of serotype a strain D7S were generated. ...
more infohttp://umu.diva-portal.org/smash/record.jsf?pid=diva2%3A1213639&c=47&searchType=SIMPLE&language=sv&query=&af=%5B%5D&aq=%5B%5B%7B%22categoryId%22%3A%2211659%22%7D%5D%5D&aq2=%5B%5B%5D%5D&aqe=%5B%5D&noOfRows=50&sortOrder=author_sort_asc&sortOrder2=title_sort_asc&onlyFullText=false&sf=all

Cytotoxic mechanisms of Aggregatibacter actinomycetemcomitans leukotoxin - Angela BrownCytotoxic mechanisms of Aggregatibacter actinomycetemcomitans leukotoxin - Angela Brown

Aggregatibacter actinomycetemcomitans is a Gram-negative pathogen that is the etiologic agent of localized aggressive ... Cytotoxic mechanisms of Aggregatibacter actinomycetemcomitans leukotoxin Brown, Angela C. Lehigh University, Bethlehem, PA, ... Cytotoxic mechanisms of Aggregatibacter actinomycetemcomitans leukotoxin. Brown, Angela C. / Lehigh University. $279,310. ... Cytotoxic mechanisms of Aggregatibacter actinomycetemcomitans leukotoxin. Brown, Angela C. / Lehigh University. $246,691. ...
more infohttp://grantome.com/grant/NIH/R00-DE022795-03

Cardiobacterium Workup: Laboratory Studies, Imaging StudiesCardiobacterium Workup: Laboratory Studies, Imaging Studies

Aggregatibacter actinomycetemcomitans, Aggregatibacter aphrophilus, C hominis, Eikenella corrodens, and Kingella kingae), which ...
more infohttps://emedicine.medscape.com/article/214021-workup

KEGG GENOME: Aggregatibacter actinomycetemcomitans D11S-1KEGG GENOME: Aggregatibacter actinomycetemcomitans D11S-1

Genome sequence of Aggregatibacter actinomycetemcomitans serotype c strain D11S-1.. Journal. J Bacteriol 191:7378-9 (2009). DOI ... Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; Pasteurellaceae; Aggregatibacter. Data source. GenBank (Assembly ...
more infohttp://www.genome.jp/kegg-bin/show_organism?org=aat

Aggregatibacter actinomycetemcomitansAggregatibacter actinomycetemcomitans

... It has been a long-held supposition of many doctors and researchers that dental ... Aggregatibacter actinomycetemcomitans-induced hypercitrullination links periodontal infection to autoimmunity in rheumatoid ... Aggregatibacter actinomycetemcomitans (Aa), is one of a number of organisms contributing to periodontal gum disease that can ...
more infohttps://www.roadback.org/research/aggregatibacter-actinomycetemcomitans/

KEGG PATHWAY: Mismatch repair - Aggregatibacter actinomycetemcomitans D11S-1KEGG PATHWAY: Mismatch repair - Aggregatibacter actinomycetemcomitans D11S-1

Mismatch repair - Aggregatibacter actinomycetemcomitans D11S-1 [ Pathway menu , Organism menu , Pathway entry , Download KGML ...
more infohttp://www.genome.jp/kegg-bin/show_pathway?aat03430+D11S_1722

Avaliação qualitativa, quantitativa e genotípica de Aggregatibacter actinomycetemcomitans...Avaliação qualitativa, quantitativa e genotípica de Aggregatibacter actinomycetemcomitans...

Aggregatibacter actinomycetemcomitans e Fusobacterium nucleatum são microrganismos gram-negativos presentes nos processos ... Aggregatibacter actinomycetemcomitans. Fusobacterium nucleatum. Biofilme subgengival. Gengivite. Periodontite crônica. Resumo ... Aggregatibacter actinomycetemcomitans. Fusobacterium nucleatum. Chronic periodontitis. Gingivitis. Subgingival biofilm. Resumo ... Aggregatibacter actinomycetemcomitans e Fusobacterium nucleatum são microrganismos gram-negativos presentes nos processos ...
more infohttp://www.teses.usp.br/teses/disponiveis/42/42132/tde-26082015-191740/pt-br.php

Gangliosides block Aggregatibacter Actinomycetemcomitans leukotoxin (LtxA)-mediated hemolysis. | Sigma-AldrichGangliosides block Aggregatibacter Actinomycetemcomitans leukotoxin (LtxA)-mediated hemolysis. | Sigma-Aldrich

Gangliosides block Aggregatibacter Actinomycetemcomitans leukotoxin (LtxA)-mediated hemolysis.. [Michael S Forman, Jason B ... Aggregatibacter actinomycetemcomitans is an oral pathogen and etiologic agent of localized aggressive periodontitis. The ... A. actinomycetemcomitans produces leukotoxin (LtxA), an important virulence factor that targets white blood cells (WBCs) and ... actinomycetemcomitans. In this study, we further investigated how LtxA might recognize and lyse RBCs. We found that, in ...
more infohttps://www.sigmaaldrich.com/catalog/papers/22069577

Toxins | Free Full-Text | Gangliosides Block Aggregatibacter Actinomycetemcomitans Leukotoxin (LtxA)-Mediated HemolysisToxins | Free Full-Text | Gangliosides Block Aggregatibacter Actinomycetemcomitans Leukotoxin (LtxA)-Mediated Hemolysis

A. actinomycetemcomitans produces leukotoxin (LtxA), an important virulence factor that targets white blood cells (WBCs) and ... actinomycetemcomitans. In this study, we further investigated how LtxA might recognize and lyse RBCs. We found that, in ... Aggregatibacter actinomycetemcomitans is an oral pathogen and etiologic agent of localized aggressive periodontitis. The ... Gangliosides Block Aggregatibacter Actinomycetemcomitans Leukotoxin (LtxA)-Mediated Hemolysis Michael S. Forman, Jason B. ...
more infohttps://www.mdpi.com/2072-6651/2/12/2824/xml

Phosphorylcholine is located in Aggregatibacter actinomycetemcomitans fimbrial protein Flp 1Phosphorylcholine is located in Aggregatibacter actinomycetemcomitans fimbrial protein Flp 1

... Ihalin, Riikka Umeå University, ... Our aims were to determine the frequency of ChoP display among Aggregatibacter actinomycetemcomitans strains, to clarify which ... Aggregatibacter actinomycetemcomitans, Serotypes, Periodontitis, Phosphorylcholine, Fimbriae, Flp1, C-reactive protein, Serum ... It further seems that ChoP-positivity does not enhance but may reduce A. actinomycetemcomitans susceptibility to serum killing. ...
more infohttp://umu.diva-portal.org/smash/record.jsf?pid=diva2%3A1268262&c=3&searchType=LIST_LATEST&language=en&query=&af=%5B%5D&aq=%5B%5B%5D%5D&aq2=%5B%5B%5D%5D&aqe=%5B%5D&noOfRows=50&sortOrder=author_sort_asc&sortOrder2=title_sort_asc&onlyFullText=false&sf=all

Oil Essential Mouthwashes Antibacterial Activity against Aggregatibacter actinomycetemcomitans: A Comparison between...Oil Essential Mouthwashes Antibacterial Activity against Aggregatibacter actinomycetemcomitans: A Comparison between...

nov., Aggregatibacter aphrophilus comb. nov. and Aggregatibacter segnis comb. nov., and emended description of Aggregatibacter ... Aggregatibacter actinomycetemcomitans (Aa) is a gram-negative coccobacillus, facultative anaerobe, closely associated with the ... S. C. Kachlany, "Aggregatibacter actinomycetemcomitans leukotoxin: from threat to therapy," Journal of Dental Research, vol. 89 ... C. Y. Wang, H. C. Wang, J. M. Li et al., "Invasive infections of Aggregatibacter (Actinobacillus) Actinomycetemcomitans," ...
more infohttps://www.hindawi.com/journals/ijd/2013/164267/

Small Things Considered: Aggregatibacter actinomycetemcomitans: A Unique Exotoxin-Producing Oral BacteriumSmall Things Considered: Aggregatibacter actinomycetemcomitans: A Unique Exotoxin-Producing Oral Bacterium

... the Gram-negative Aggregatibacter actinomycetemcomitans. For readers with an interest in taxonomy, this microbe was originally ... Aggregatibacter actinomycetemcomitans: A Unique Exotoxin-Producing Oral Bacterium by Pedro Valero-Guillén ... Zijnge V, Kieselbach T, & Oscarsson J (2012). Proteomics of protein secretion by Aggregatibacter actinomycetemcomitans. PloS ... The Toxins of A. actinomycetemcomitans. It has been known for quite some time that A. actinomycetemcomitans makes two toxins, ...
more infohttps://schaechter.asmblog.org/schaechter/2013/07/aggregatibacter-actinomycetemcomitans-a-unique-exotoxin-producing-oral-bacterium-.html

Aggregatibacter actinomycetemcomitans - WikipediaAggregatibacter actinomycetemcomitans - Wikipedia

Aggregatibacter actinomycetemcomitans (previously Actinobacillus actinomycetemcomitans) is a Gram-negative, facultative ... nov., Aggregatibacter aphrophilus comb. nov. and Aggregatibacter segnis comb. nov., and emended description of Aggregatibacter ... Type strain of Aggregatibacter actinomycetemcomitans at BacDive - the Bacterial Diversity Metadatabase. ... It was reclassified as Actinobacillus actinomycetemcomitans by Topley & Wilson (1929) and as Haemophilus actinomycetemcomitans ...
more infohttps://en.wikipedia.org/wiki/Aggregatibacter_actinomycetemcomitans

CF65 02195 - Oxaloacetate decarboxylase alpha subunit,putative - Aggregatibacter actinomycetemcomitans HK1651 - CF65 02195 gene...CF65 02195 - Oxaloacetate decarboxylase alpha subunit,putative - Aggregatibacter actinomycetemcomitans HK1651 - CF65 02195 gene...

tr,X2JQV5,X2JQV5_AGGAC Oxaloacetate decarboxylase alpha subunit,putative OS=Aggregatibacter actinomycetemcomitans HK1651 OX= ... cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Aggregatibacter › ...
more infohttps://www.uniprot.org/uniprot/X2JQV5

Aggregatibacter actinomycetemcomitans-induced hypercitrullination links periodontal infection to autoimmunity in rheumatoid...Aggregatibacter actinomycetemcomitans-induced hypercitrullination links periodontal infection to autoimmunity in rheumatoid...

Among these, Aggregatibacter actinomycetemcomitans (Aa), but not other candidate pathogens, induced hypercitrullination in host ... Aggregatibacter actinomycetemcomitans generates citrullinated autoantigens involved in rheumatoid arthritis through its pore- ... Aggregatibacter actinomycetemcomitans generates citrullinated autoantigens involved in rheumatoid arthritis through its pore- ... Aggregatibacter actinomycetemcomitans-induced hypercitrullination links periodontal infection to autoimmunity in rheumatoid ...
more infohttps://stm.sciencemag.org/content/8/369/369ra176

DIGITAL.CSIC: Variability in the response of human dendritic cells stimulated with Porphyromonas gingivalis or Aggregatibacter...DIGITAL.CSIC: Variability in the response of human dendritic cells stimulated with Porphyromonas gingivalis or Aggregatibacter...

... actinomycetemcomitans. Although both pathogens induced a T helper 1 pattern of cytokine expression, A. actinomycetemcomitans- ... Results: Both P. gingivalis and A. actinomycetemcomitans led to dendritic cell maturation, but the expression of CD80 was ... While 106 bacteria/mL of P. gingivalis or 104 bacteria/mL of A. actinomycetemcomitans induced expression of interleukin-12p40, ... Conclusion: These results demonstrate that A. actinomycetemcomitans is a more potent immunogen than P. gingivalis because, at ...
more infohttps://digital.csic.es/handle/10261/56113

Utilization of Variant and Fusion Proteins To Functionally Map the Aggregatibacter actinomycetemcomitans Trimeric...Utilization of Variant and Fusion Proteins To Functionally Map the Aggregatibacter actinomycetemcomitans Trimeric...

Utilization of Variant and Fusion Proteins To Functionally Map the Aggregatibacter actinomycetemcomitans Trimeric ... Utilization of Variant and Fusion Proteins To Functionally Map the Aggregatibacter actinomycetemcomitans Trimeric ... Utilization of Variant and Fusion Proteins To Functionally Map the Aggregatibacter actinomycetemcomitans Trimeric ... Utilization of Variant and Fusion Proteins To Functionally Map the Aggregatibacter actinomycetemcomitans Trimeric ...
more infohttps://iai.asm.org/content/86/3/e00697-17/figures-only

Bdellovibrio bacteriovorus attacks Aggregatibacter actinomycetemcomitansBdellovibrio bacteriovorus attacks Aggregatibacter actinomycetemcomitans

... For figures, tables and references we refer the reader ... Aggregatibacter actinomycetemcomitans is considered to be a key periodontopathogen and has been associated with aggressive ... Predation of A. actinomycetemcomitans Biofilms The SEM images (Fig. 3⇓) show the breakdown of an A. actinomycetemcomitans 2751 ... Similar to the experiments with planktonic A. actinomycetemcomitans, Bdellovibrio was able to attack A. actinomycetemcomitans ...
more infohttp://dentisty.org/bdellovibrio-bacteriovorus-attacks-aggregatibacter-actinomycet.html

Microbiology Society Journals | Inner-membrane protein MorC is involved in fimbriae production and biofilm formation in...Microbiology Society Journals | Inner-membrane protein MorC is involved in fimbriae production and biofilm formation in...

These results suggest that MorC influences fimbrial secretion and microcolony formation in A. actinomycetemcomitans. ... secretion and assembly on the surface of the periodontal pathogen Aggregatibacter actinomycetemcomitans are essential for ... Tang G. , Mintz K. P. (2010). Glycosylation of the collagen adhesin EmaA of Aggregatibacter actinomycetemcomitans is dependent ... Smith K. P. (2015). A conserved inner membrane protein of Aggregatibacter actinomycetemcomitans is integral for membrane ...
more infohttp://mic.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000246

Positive Tested Species Reactivity: Aggregatibacter-actinomycetemcomitans, ManducaPositive Tested Species Reactivity: Aggregatibacter-actinomycetemcomitans, Manduca

Hybridoma technology is used to fuse fusion a B cell and myeloma to form a hybridoma that produces identical monoclonal antibodies.
more infohttps://dshb.biology.uiowa.edu/monoclonal/custitem34/Aggregatibacter-actinomycetemcomitans,Manduca

Positive Tested Species Reactivity: Aggregatibacter-actinomycetemcomitans, RodentPositive Tested Species Reactivity: Aggregatibacter-actinomycetemcomitans, Rodent

Hybridoma technology is used to fuse fusion a B cell and myeloma to form a hybridoma that produces identical monoclonal antibodies.
more infohttps://dshb.biology.uiowa.edu/monoclonal/custitem34/Aggregatibacter-actinomycetemcomitans,Rodent
  • Material and Methods: Using different concentrations of P. gingivalis ATCC 33277 and A. actinomycetemcomitans ATCC 33384, we determined the expression of the maturation markers CD80 and CD86 from purified human dendritic cells by flow cytometry. (csic.es)
  • We recently discovered that LtxA can also lyse red blood cells (RBCs) and hemolysis may be important for pathogenesis of A. actinomycetemcomitans. (sigmaaldrich.com)
  • The pore-forming toxin LtxA of A. actinomycetemcomitans may be a trigger of the autoimmune disease rheumatoid arthritis due to its ability to stimulate protein citrullination, a post-translational protein modification targeted by autoantibodies in this disease. (wikipedia.org)
  • It has been known for quite some time that A. actinomycetemcomitans makes two toxins, the leucotoxin (LtxA) and the c ytolethal d istending t oxin (CDT), to trigger disease. (asmblog.org)
  • Although both pathogens induced a T helper 1 pattern of cytokine expression, A. actinomycetemcomitans-stimulated dendritic cells expressed interleukin-1β, interleukin-12, interferon-γ, tumor necrosis factor-α and tumor necrosis factor-β at lower bacterial concentrations than P. gingivalis. (csic.es)
  • Light-induced activity of the targeted photosensitizer reduced the viability of A. actinomycetemcomitans biofilm, as indicated by membrane permeability to propidium iodide. (uzh.ch)
  • The toxin kills human and primate white blood cells and likely plays a role in A. actinomycetemcomitans evasion of the immune response during infection. (grantome.com)
  • Conclusion: These results demonstrate that A. actinomycetemcomitans is a more potent immunogen than P. gingivalis because, at least in vitro, it induces stronger differentiation and activation of dendritic cells. (csic.es)
  • The aim of this work is to determine the antibacterial activity of three marketed mouthwashes on suspended and sessile states of Aggregatibacter actinomycetemcomitans . (hindawi.com)