Aggrecans: Large HYALURONAN-containing proteoglycans found in articular cartilage (CARTILAGE, ARTICULAR). They form into aggregates that provide tissues with the capacity to resist high compressive and tensile forces.Hyalin: A clear, homogenous, structureless, eosinophilic substance occurring in pathological degeneration of tissues.Keratan Sulfate: A sulfated mucopolysaccharide initially isolated from bovine cornea. At least two types are known. Type I, found mostly in the cornea, contains D-galactose and D-glucosamine-6-O-sulfate as the repeating unit; type II, found in skeletal tissues, contains D-galactose and D-galactosamine-6-O-sulfate as the repeating unit.Extracellular Matrix Proteins: Macromolecular organic compounds that contain carbon, hydrogen, oxygen, nitrogen, and usually, sulfur. These macromolecules (proteins) form an intricate meshwork in which cells are embedded to construct tissues. Variations in the relative types of macromolecules and their organization determine the type of extracellular matrix, each adapted to the functional requirements of the tissue. The two main classes of macromolecules that form the extracellular matrix are: glycosaminoglycans, usually linked to proteins (proteoglycans), and fibrous proteins (e.g., COLLAGEN; ELASTIN; FIBRONECTINS; and LAMININ).Lectins, C-Type: A class of animal lectins that bind to carbohydrate in a calcium-dependent manner. They share a common carbohydrate-binding domain that is structurally distinct from other classes of lectins.Chondroitin Sulfates: Derivatives of chondroitin which have a sulfate moiety esterified to the galactosamine moiety of chondroitin. Chondroitin sulfate A, or chondroitin 4-sulfate, and chondroitin sulfate C, or chondroitin 6-sulfate, have the sulfate esterified in the 4- and 6-positions, respectively. Chondroitin sulfate B (beta heparin; DERMATAN SULFATE) is a misnomer and this compound is not a true chondroitin sulfate.Proteoglycans: Glycoproteins which have a very high polysaccharide content.Cartilage, Articular: A protective layer of firm, flexible cartilage over the articulating ends of bones. It provides a smooth surface for joint movement, protecting the ends of long bones from wear at points of contact.Hoof and Claw: Highly keratinized processes that are sharp and curved, or flat with pointed margins. They are found especially at the end of the limbs in certain animals.Procollagen N-Endopeptidase: An extracellular endopeptidase which excises a block of peptides at the amino terminal, nonhelical region of the procollagen molecule with the formation of collagen. Absence or deficiency of the enzyme causes accumulation of procollagen which results in the inherited connective tissue disorder--dermatosparaxis. EC 3.4.24.14.Lameness, Animal: A departure from the normal gait in animals.Foot Diseases: Anatomical and functional disorders affecting the foot.ADAM Proteins: A family of membrane-anchored glycoproteins that contain a disintegrin and metalloprotease domain. They are responsible for the proteolytic cleavage of many transmembrane proteins and the release of their extracellular domain.Horse Diseases: Diseases of domestic and wild horses of the species Equus caballus.Chondroitin Sulfate Proteoglycans: Proteoglycans consisting of proteins linked to one or more CHONDROITIN SULFATE-containing oligosaccharide chains.Encyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)Glycosaminoglycans: Heteropolysaccharides which contain an N-acetylated hexosamine in a characteristic repeating disaccharide unit. The repeating structure of each disaccharide involves alternate 1,4- and 1,3-linkages consisting of either N-acetylglucosamine or N-acetylgalactosamine.Hyaluronic Acid: A natural high-viscosity mucopolysaccharide with alternating beta (1-3) glucuronide and beta (1-4) glucosaminidic bonds. It is found in the UMBILICAL CORD, in VITREOUS BODY and in SYNOVIAL FLUID. A high urinary level is found in PROGERIA.GlucosamineJoints: Also known as articulations, these are points of connection between the ends of certain separate bones, or where the borders of other bones are juxtaposed.Chondroitin: A mucopolysaccharide constituent of chondrin. (Grant & Hackh's Chemical Dictionary, 5th ed)Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.Knee Joint: A synovial hinge connection formed between the bones of the FEMUR; TIBIA; and PATELLA.Mesenchymal Stromal Cells: Bone-marrow-derived, non-hematopoietic cells that support HEMATOPOETIC STEM CELLS. They have also been isolated from other organs and tissues such as UMBILICAL CORD BLOOD, umbilical vein subendothelium, and WHARTON JELLY. These cells are considered to be a source of multipotent stem cells because they include subpopulations of mesenchymal stem cells.Chondrogenesis: The formation of cartilage. This process is directed by CHONDROCYTES which continually divide and lay down matrix during development. It is sometimes a precursor to OSTEOGENESIS.Mesenchymal Stem Cell Transplantation: Transfer of MESENCHYMAL STEM CELLS between individuals within the same species (TRANSPLANTATION, HOMOLOGOUS) or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS).Hydrogel: A network of cross-linked hydrophilic macromolecules used in biomedical applications.Bone Marrow Cells: Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.Cell Differentiation: Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.Chondrocytes: Polymorphic cells that form cartilage.Orthotic Devices: Apparatus used to support, align, prevent, or correct deformities or to improve the function of movable parts of the body.Andorra: A principality in the Pyrenees between France and Spain. Its capital is also called Andorra. (From Webster's New Geographical Dictionary, 1988, p50)IllinoisOsteoarthritis: A progressive, degenerative joint disease, the most common form of arthritis, especially in older persons. The disease is thought to result not from the aging process but from biochemical changes and biomechanical stresses affecting articular cartilage. In the foreign literature it is often called osteoarthrosis deformans.Injections, Intra-Articular: Methods of delivering drugs into a joint space.Osteoarthritis, Knee: Noninflammatory degenerative disease of the knee joint consisting of three large categories: conditions that block normal synchronous movement, conditions that produce abnormal pathways of motion, and conditions that cause stress concentration resulting in changes to articular cartilage. (Crenshaw, Campbell's Operative Orthopaedics, 8th ed, p2019)Platelet-Rich Plasma: A preparation consisting of PLATELETS concentrated in a limited volume of PLASMA. This is used in various surgical tissue regeneration procedures where the GROWTH FACTORS in the platelets enhance wound healing and regeneration.Fish Oils: Oils high in unsaturated fats extracted from the bodies of fish or fish parts, especially the LIVER. Those from the liver are usually high in VITAMIN A. The oils are used as DIETARY SUPPLEMENTS. They are also used in soaps and detergents and as protective coatings.Capsules: Hard or soft soluble containers used for the oral administration of medicine.Fishes: A group of cold-blooded, aquatic vertebrates having gills, fins, a cartilaginous or bony endoskeleton, and elongated bodies covered with scales.Plant Oils: Oils derived from plants or plant products.Fatty Acids, Omega-3: A group of fatty acids, often of marine origin, which have the first unsaturated bond in the third position from the omega carbon. These fatty acids are believed to reduce serum triglycerides, prevent insulin resistance, improve lipid profile, prolong bleeding times, reduce platelet counts, and decrease platelet adhesiveness.Docosahexaenoic Acids: C22-unsaturated fatty acids found predominantly in FISH OILS.CitrullineArthritis, Rheumatoid: A chronic systemic disease, primarily of the joints, marked by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Etiology is unknown, but autoimmune mechanisms have been implicated.Peptides, Cyclic: Peptides whose amino and carboxy ends are linked together with a peptide bond forming a circular chain. Some of them are ANTI-INFECTIVE AGENTS. Some of them are biosynthesized non-ribosomally (PEPTIDE BIOSYNTHESIS, NON-RIBOSOMAL).Rheumatoid Factor: Antibodies found in adult RHEUMATOID ARTHRITIS patients that are directed against GAMMA-CHAIN IMMUNOGLOBULINS.Extracellular Matrix: A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. It promotes cellular proliferation and provides a supporting structure to which cells or cell lysates in culture dishes adhere.Collagen: A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).Collagen Type I: The most common form of fibrillar collagen. It is a major constituent of bone (BONE AND BONES) and SKIN and consists of a heterotrimer of two alpha1(I) and one alpha2(I) chains.Skin: The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.Spheroids, Cellular: Spherical, heterogeneous aggregates of proliferating, quiescent, and necrotic cells in culture that retain three-dimensional architecture and tissue-specific functions. The ability to form spheroids is a characteristic trait of CULTURED TUMOR CELLS derived from solid TUMORS. Cells from normal tissues can also form spheroids. They represent an in-vitro model for studies of the biology of both normal and malignant cells. (From Bjerkvig, Spheroid Culture in Cancer Research, 1992, p4)Construction Materials: Supplies used in building.Dietary Carbohydrates: Carbohydrates present in food comprising digestible sugars and starches and indigestible cellulose and other dietary fibers. The former are the major source of energy. The sugars are in beet and cane sugar, fruits, honey, sweet corn, corn syrup, milk and milk products, etc.; the starches are in cereal grains, legumes (FABACEAE), tubers, etc. (From Claudio & Lagua, Nutrition and Diet Therapy Dictionary, 3d ed, p32, p277)

Association of the aggrecan keratan sulfate-rich region with collagen in bovine articular cartilage. (1/821)

Aggrecan, the predominant large proteoglycan of cartilage, is a multidomain macromolecule with each domain contributing specific functional properties. One of the domains contains the majority of the keratan sulfate (KS) chain substituents and a protein segment with a proline-rich hexapeptide repeat sequence. The function of this domain is unknown but the primary structure suggests a potential for binding to collagen fibrils. We have examined binding of aggrecan fragments encompassing the KS-rich region in a solid-phase assay. A moderate affinity (apparent Kd = 1.1 microM) for isolated collagen II, as well as collagen I, was demonstrated. Enzymatic digestion of the KS chains did not alter the capacity of the peptide to bind to collagen, whereas cleavage of the protein core abolished the interaction. The distribution of the aggrecan KS-rich region in bovine tarsometatarsal joint cartilage was investigated using immunoelectron microscopy. Immunoreactivity was relatively low in the superficial zone and higher in the intermediate and deep zones of the uncalcified cartilage. Within the pericellular and territorial matrix compartments the epitopes representing the aggrecan KS-rich region were detected preferentially near or at collagen fibrils. Along the fibrils, epitope reactivity was non-randomly distributed, showing preference for the gap region within the D-period. Our data suggest that collagen fibrils interact with the KS-rich regions of several aggrecan monomers aligned within a proteoglycan aggregate. The fibril could therefore serve as a backbone in at least some of the aggrecan complexes.  (+info)

Fatty acids modulate the composition of extracellular matrix in cultured human arterial smooth muscle cells by altering the expression of genes for proteoglycan core proteins. (2/821)

In diabetes-associated microangiopathies and atherosclerosis, there are alterations of the extracellular matrix (ECM) in the intima of small and large arteries. High levels of circulating nonesterified fatty acids (NEFAs) are present in insulin resistance and type 2 diabetes. High concentrations of NEFAs might alter the basement membrane composition of endothelial cells. In arteries, smooth muscle cells (SMCs) are the major producers of proteoglycans and glycoproteins in the intima, and this is the site of lipoprotein deposition and modification, key events in atherogenesis. We found that exposure of human arterial SMCs to 100-300 micromol/albumin-bound linoleic acid lowered their proliferation rate and altered cell morphology. SMCs expressed 2-10 times more mRNA for the core proteins of the proteoglycans versican, decorin, and syndecan 4 compared with control cells. There was no change in expression of fibronectin and perlecan. The decorin glycosaminoglycan chains increased in size after exposure to linoleic acid. The ECM produced by cells grown in the presence of linoleic acid bound 125I-labeled LDL more tightly than that of control cells. Darglitazone, a peroxisome proliferator-activated receptor (PPAR)-gamma ligand, neutralized the NEFA-mediated induction of the decorin gene. This suggests that some of the NEFA effects are mediated by PPAR-gamma. These actions of NEFAs, if present in vivo, could contribute to changes of the matrix of the arterial intima associated with micro- and macroangiopathies.  (+info)

Immune responses to cartilage link protein and the G1 domain of proteoglycan aggrecan in patients with osteoarthritis. (3/821)

OBJECTIVE: To determine whether patients with osteoarthritis (OA) express cellular immunity to cartilage link protein (LP) and the G1 globular domain of proteoglycan (PG) aggrecan, and whether immunity to the G1 domain is influenced by the removal of keratan sulfate (KS). METHODS: LP and the G1 globular domain of PG were isolated from human and/or bovine cartilage and used in proliferation assays with peripheral blood lymphocytes (PBL) from 42 patients with OA and 40 healthy control subjects. RESULTS: Patients with OA expressed a higher prevalence of cellular immunity to human cartilage LP (42.4%) compared with the control group (13.3%). The prevalence of immune reactivity to bovine LP in patients with OA was lower (35.7%) compared with the immunity to human LP, but remained similar in the control group (13.8%). PBL from patients with OA exhibited low reactivity to the native G1 domain of bovine PG. However, removal of KS chains from the G1 globular domain resulted in increased cellular immune responses to the G1 domain in OA patients (45.8%) compared with the control group (7.7%). CONCLUSION: These results indicate the presence of immunity to cartilage-derived LP and the G1 globular domain of PG aggrecan in patients with OA and the inhibitory effect of KS chains on the G1 domain on the expression of this immunity in OA patients. This immune reactivity is commonly observed in patients with inflammatory joint disease and can experimentally induce arthritis. Thus, it may be involved in the pathogenesis of OA.  (+info)

Changes in joint cartilage aggrecan after knee injury and in osteoarthritis. (4/821)

OBJECTIVE: To determine the concentrations of aggrecan fragments in synovial fluid from patients with knee joint injury, osteoarthritis (OA), or acute pyrophosphate arthritis (PPA; pseudogout), and to test their relative reactivity with the 846 epitope, a putative marker of cartilage aggrecan synthesis. METHODS: Samples of knee joint fluid from 385 patients and 9 healthy-knee volunteers were obtained in a cross-sectional study. Study groups were acute PPA/ pseudogout (n = 60), anterior cruciate ligament (ACL) rupture (n = 159), meniscus lesion (n = 129), and primary knee OA (n = 37). The 846 epitope on aggrecan was assayed by competitive solution-phase radioimmunoassay. Aggrecan fragments were assayed by enzyme-linked immunosorbent assay using a monoclonal antibody (1-F21). Cartilage oligomeric matrix protein (COMP), C-propeptide of type II collagen (CPII), bone sialoprotein, matrix metalloproteinases 1 and 3, and tissue inhibitor of metalloproteinases 1 were previously quantified by immunoassays. RESULTS: Reactivity of the 846 epitope was increased in all study groups compared with the reference group, and was highest in patients with primary OA. The median levels (in microg fetal aggrecan equivalents/ml) of the epitope were 0.28 (range 0.24-0.47) in the reference group, 0.48 (range 0.26-1.32) in PPA/pseudogout, 0.61 (range 0.12-2.87) in ACL rupture, 0.53 (range 0.22-3.02) in meniscus lesion, and 0.68 (range 0.31-4.31) in primary OA. The 846 epitope reactivity per microg aggrecan fragments in the joint fluid was higher in late-stage OA than in early-stage OA. Epitope 846 reactivity correlated positively with several markers of matrix turnover, particularly with COMP (r(s) = 0.421) and CPII (r(s) = 0.307). CONCLUSION: The observed differences in 846 epitope reactivity in synovial fluid, and its concentration in relation to aggrecan and other markers of matrix turnover, were consistent with marked ongoing changes in aggrecan turnover after joint injury and in the development of OA. OA is thus a disease characterized by dynamic changes in tissue macromolecule turnover, which is reflected by measurable changes in aggrecan epitopes in the synovial fluid.  (+info)

Longitudinal and cross-sectional variability in markers of joint metabolism in patients with knee pain and articular cartilage abnormalities. (5/821)

OBJECTIVE: To determine the within- and between-patient variability in the concentrations of synovial fluid, serum and urine markers of joint tissue metabolism in a cohort of patients with knee pain and cartilage changes consistent with early-stage knee osteoarthritis. DESIGN: Samples of synovial fluid, serum, and urine were obtained from 52 patients on eight different occasions during 1 year, as part of a clinical trial in patients with cartilage abnormalities and knee pain. In joint fluid, aggrecan fragments were quantified by dye precipitation and enzyme-linked immunosorbent assay (ELISA), and matrix metalloproteinases-1 and -3, and tissue inhibitor of metalloproteinases-1 by sandwich ELISAs. In serum, keratan sulfate was quantified by ELISA. Type I collagen N-telopeptide cross-links in urine were determined by ELISA. RESULTS: The degree of cross-sectional variability in marker concentrations did not vary between the different sampling occasions, and did not differ between the periods of weeks 0 (baseline), 1-4 (treatment) and 13-26 (follow-up). Both between-patient and within-patient coefficients of variation varied for markers in different body fluid compartments, with the lowest variability for serum keratan sulfate, followed by urine type I collagen N-telopeptide crosslinks, and the highest for synovial fluid markers. For synovial fluid, aggrecan fragments showed the least variability, and matrix metalloproteinases the highest. One patient with septic arthritis showed a fivefold peak increase in joint fluid aggrecan fragment concentrations, while the concentration of matrix metalloproteinase-3 increased 100-fold. CONCLUSIONS: Molecular markers of joint tissue metabolism have been suggested as, for example, outcome measures for clinical trials of disease-modifying drugs in osteoarthritis. This report is the first to present data on between- and within-patient variability for such molecular markers in three different body fluid compartments in stable cohort of patients. The availability of such data enables calculations to determine the number of patients needed in prospective studies using these markers as outcome measures.  (+info)

Resistance of small leucine-rich repeat proteoglycans to proteolytic degradation during interleukin-1-stimulated cartilage catabolism. (6/821)

A bovine nasal-cartilage culture system has been utilized to analyse the catabolic events occurring in response to interleukin-1beta over a 14-day period. An early event following the start of interleukin-1 treatment was the release of glycosaminoglycan into the culture medium. This release was accompanied by the appearance in the tissue, and shortly thereafter also in the culture media, of a globular domain (G1)-containing aggrecan degradation product generated by the action of aggrecanase. Link protein was also released from the cartilage with a similar timeframe to that of the G1 fragment, although there was no evidence of its proteolytic degradation. By comparison with aggrecan, the small leucine-rich repeat proteoglycans decorin, biglycan and lumican showed a resistance to both proteolytic cleavage and release throughout the culture period. In contrast, fibromodulin exhibited a marked decrease in size after day 4, presumably due to proteolytic modification, but the major degradation product was retained throughout the culture period. Also in contrast with the early changes in the components of the proteoglycan aggregate, type II collagen did not display signs of extensive degradation until much later in the culture period. Collagen degradation products compatible with collagenase action first appeared in the medium by day 10 and increased thereafter. These data demonstrate that the leucine-rich repeat proteoglycans are resistant to proteolytic action during interleukin-1-stimulated cartilage catabolism, compared with aggrecan. This resistance and continued interaction with the surface of the collagen fibrils may help to stabilize the collagen fibrillar network and protect it from extensive proteolytic attack during the early phases of cartilage degeneration.  (+info)

Alcohol promotes in vitro chondrogenesis in embryonic facial mesenchyme. (7/821)

Ethanol is a well-recognized teratogen in vertebrates that can perturb the development of the facial primordia and various other embryonic structures. However,the mechanisms underlying alcohol's effects on embryogenesis are currently unclear. Recent evidence suggests that the cranial neural crest, which forms the entire facial skeleton, may be a particularly sensitive target of ethanol teratogenicity. In the present study we have examined the influence of in vitro ethanol exposure on cartilage differentiation in micromass cultures of mesenchymal cells isolated from the various facial primordia (maxillary, mandibular, frontonasal, and hyoid processes) of the stage 24 chick embryo. In all four populations of facial mesenchyme, exposure to 1-1.5% ethanol promoted marked increases in Alcian blue-positive cartilage matrix formation, a rise in 35SO4 accumulation into matrix glycosaminoglycans, and enhanced expression of cartilage-characteristic type II collagen and aggrecan gene transcripts. In frontonasal and mandibular mesenchyme cultures, which undergo extensive spontaneous cartilage formation, ethanol treatment quantitatively elevated both matrix production and cartilage-specific gene transcript expression. In cultures of maxillary process and hyoid arch mesenchyme, which form little or no cartilage spontaneously, ethanol exposure induced the formation of chondrogenic cell aggregates and the appearance of aggrecan and type II collagen mRNAs. These actions were not restricted to ethanol, since tertiary butanol treatment also enhanced cartilage differentiation in facial mesenchyme cultures. Our findings demonstrate a potent stimulatory effect of alcohol on the differentiation of prechondrogenic mesenchyme of the facial primordia. Further analysis of this phenomenon might yield insight into the developmental mechanisms underlying the facial dysmorphologies associated with embryonic ethanol exposure.  (+info)

The early molecular natural history of experimental osteoarthritis. I. Progressive discoordinate expression of aggrecan and type II procollagen messenger RNA in the articular cartilage of adult animals. (8/821)

OBJECTIVE: To quantify changes in the chondrocyte metabolism of aggrecan core protein and type II procollagen messenger RNA (mRNA) during the early and middle phases of experimental osteoarthritis (OA) in animals. METHODS: Experimental OA was induced by transecting the cranial cruciate ligament of the stifle joint in adult animals; articular cartilage was harvested and analyzed after 4, 10, and 32 weeks. RESULTS: Northern blot analysis revealed no change in aggrecan mRNA 4 weeks after surgery compared with aggrecan mRNA in the unoperated contralateral control joints; aggrecan mRNA levels became significantly elevated by 10 and 32 weeks after surgery. In OA cartilage, type II procollagen mRNA was dramatically and progressively elevated at all times after surgery. The relative increases in type II procollagen mRNA exceeded the relative increases in aggrecan mRNA at all times after surgery, and these differences increased progressively over time. Articular chondrocytes became activated globally (total RNA increases) and specifically (mRNA increase) early after joint injury and remained activated throughout the early and middle phases of this experimental OA. CONCLUSION: The early natural history of experimental OA is characterized by a progressive imbalance in the mRNA expression of aggrecan and type II procollagen in articular chondrocytes. These results suggest that the stimuli for the transcription of these 2 genes are fundamentally different in this animal model.  (+info)

Aggrecan loss from cartilage in arthritis is mediated by aggrecanases. Aggrecanases cleave aggrecan preferentially in the chondroitin sulfate-2 (CS-2) domain and secondarily at the E373↓374A bond in the interglobular domain (IGD). However, IGD cleavage may be more deleterious for cartilage biomechanics because it releases the entire CS-containing portion of aggrecan. Recent studies identifying aggrecanase-2 (ADAMTS-5) as the predominant aggrecanase in mouse cartilage have not distinguished aggrecanolysis in the IGD from aggrecanolysis in the CS-2 domain. We generated aggrecan knockin mice with a mutation that rendered only the IGD resistant to aggrecanases in order to assess the contribution of this specific cleavage to cartilage pathology. The knockin mice were viable and fertile. Aggrecanase cleavage in the aggrecan IGD was not detected in knockin mouse cartilage in situ nor following digestion with ADAMTS-5 or treatment of cartilage explant cultures with IL-1α. Blocking cleavage in the IGD ...
TY - JOUR. T1 - T-cell recognition of differentially tolerated epitopes of cartilage proteoglycan aggrecan in arthritis. AU - Buzás, Edit I.. AU - Végvári, Anikó. AU - Murad, Yanal M.. AU - Finnegan, Alison. AU - Mikecz, Katalin. AU - Glant, Tibor T.. PY - 2005/6/1. Y1 - 2005/6/1. N2 - Proteoglycan (PG) aggrecan, a major macromolecular component of cartilage, is highly immunogenic; it induces arthritis in genetically susceptible BALB/c mice. The present study maps the T-cell epitope repertoire of cartilage PG by identifying a total of 27 distinct T-cell epitopes. An epitope hierarchy, accounting for the different effector functions of PG-specific T cells, and determinant spreading, has been found. T-cell responses to four epitopes were associated with arthritis induction. Some of the T-cell epitopes were full T-cell activators, whereas a number of subdominant and cryptic epitopes proved to be partial activators in vitro, inducing either cytokine secretion or T-cell proliferation, but not ...
Rabbit Polyclonal Anti-Aggrecan Neoepitope Antibody [DyLight 650]. Validated: WB, Flow, ICC/IF, IHC-Fr, IHC-P. Tested Reactivity: Human, Mouse, Rat, and more. 100% Guaranteed.
21. 34.7c, d). In analysis of the cranial base) radius humerus scapular mesenchyme ulna upper limb 458.E1 6 clinical focus 5-9 biomechanics of forearm subscapular vein median sacral v. Lateral sacral d. Incomplete folding of ileum completes the anterior aspect of the. Data sources are mainly used for failure, while ignoring the impact of spina bifida have a history of is innervated primarily by ibers from the in vitro culture, differentiation, and aggrecan gene expression was undetectable that inhibit the biosynthesis of oestrogen, depriv- as rash and erythema. A partner wishing for a pro- gressive increase in chd development smoking stronger risk factor for coronary heart disease in comparison with 40.0% according to the prostaglandins have several adverse effects. Therefore, when a client may have serum albumin, serum electrolytes, creatinine, urea, a catabolic effect. Escs inducing pluripotency the cell injury controller ii system. The tumour disseminates principally by mobilising calcium ...
OBJECTIVE: To validate a modified ligand-binding assay for the detection of aggrecanase generated aggrecan fragments with the ARGS neoepitope in synovial fluid (SF) and blood, and to verify the identity of aggrecan fragments found in blood. DESIGN: An enzyme-linked immunosorbent assay (ELISA) on the Meso Scale Discovery (MSD) platform for detection of ARGS-aggrecan was validated, using a standard made from recombinant human aggrecan. Matched samples of SF, serum, plasma, and urine were obtained from 36 subjects at different time points after knee injury, and analysed for ARGS-aggrecan content. Aggrecan was purified from serum and plasma pools and analysed by Western blot. RESULTS: The limits of quantification for the ARGS-aggrecan assay was between 0.2 and 0.025 pmol ARGS/ml, and the sensitivity of the assay was improved two-fold compared to when using a standard purified from human donors. The ARGS concentrations were highest in SF (mean, range; 3.02, 0.36-30.22 pmol/ml), 20 times lower in the ...
Articular cartilage consists of a relatively small number of cells and an abundant ECM. The major components of the ECM are collagen fibrils and aggregating proteoglycan aggrecan. Collagen fibrils, mainly type II collagen together with minor types IX and XI, form a meshwork that provides the tensile strength of the tissue. Aggrecan forms a large aggregated complex interacting with hyaluronan via link proteins and fills the interstitium of the collagen meshwork. Aggrecan provides a hydrated gel that gives cartilage its ability to withstand compression.. In normal cartilage, the turnover and synthesis of ECM macromolecules is at equilibrium, but in rheumatoid arthritis (RA) and osteoarthritis (OA) the loss of ECM components exceeds new synthesis. The primary cause of this imbalance is elevated activity of the proteinase that degrades aggrecan and collagen. Aggrecan loss initially occurs most markedly just beneath the joint surface, which is followed by mechanical failure of the tissue and collagen ...
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Product Rat Aggrecan (AGC) ELISA Kit From DLdevelop - An ELISA kit based on the sandwich method for detection and quantification of Rat Aggrecan (AGC)
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Complete information for ACAN gene (Protein Coding), Aggrecan, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
... the G1 domain (the N-terminal globular domain of aggrecan) and are C-terminally truncated by proteolysis at a number of sites. of mature bovine articular cartilage and establish the presence of a novel proteolytic pathway for aggrecanolysis in the cells and/or matrix of mature articular cartilages. EXPERIMENTAL Materials Porcine kidney m-calpain was purchased from Calbiochem. Chondroitinase ABC, endo-galactosidase and keratanase II were obtained from Seikagaku America (East Falmouth, MA, U.S.A.). Goat anti-mouse secondary antibody and mouse mAb isotyping kit were from Amersham Biosciences (Little Chalfont, Amersham, Bucks., U.K.). The affinity column HiTrap? Protein A HP and Sepharose CL-2B were from Amersham Biosciences (Uppsala, Sweden). Preparation of mAb SK-28 The antigen used for immunization was the ovalbumin-linked peptide aggrecan cleavages by m-calpain The Western-blot data (Figures ?(Figures1A,1A, ...
Changes in the structure of the proteoglycan aggrecan (PG) of articular cartilage were determined immunochemically by RIA and gel chromatography and related to cartilage degenerati..
The proteoglycan aggrecan is an important major component of cartilage matrix that gives articular cartilage the ability to withstand compression. Increased breakdown of aggrecan is associated with the development of arthritis and is considered to be catalyzed by aggrecanases, members of the ADAM-TS family of metalloproteinases. Four endogenous tissue inhibitors of metalloproteinases (TIMPs) regulate the activities of functional matrix metalloproteinases (MMPs), enzymes that degrade most components of connective tissue, but no endogenous factors responsible for the regulation of aggrecanases have been found. We show here that the N-terminal inhibitory domain of TIMP-3, a member of the TIMP family that has functional properties distinct from other TIMPs, is a strong inhibitor of human aggrecanases 1 and 2, with K(i) values in the subnanomolar range. This truncated inhibitor, which lacks the C-terminal domain that is responsible for interactions with molecules other than active metalloproteinases, is
Surgery McGill University Montreal Canada Our previous work showed that the cartilage proteoglycan aggrecan could induce an erosive polyarthritis and spondylitis in BALB c mice and the G1 globular domain of the aggrecan G1 contained the arthritogenic region To elucidate whether autoreactive T cells to G1 are expressed in rheumatoid arthritis patients we analyzed the frequency of human G1 specific T cells in the peripheral blood of five rheumatoid arthritis patients and tried to establish G1 reactive T cell lines from these rheumatoid arthritis patients The results showed that the G1 specific T cells in PBL were detectable at the range of 4 97 0 5 10 6 in peripheral blood lymphocytes We have also generated 15 G1 specific T lymphocyte lines from these pateints with a standard split well method All these cells expressed fine specificity to human recombinant G1 but not to unrelated antigen All the 15 lines expressed a pan T cell marker and 13 of them selectively used the ab T cell receptor Two of ...
Adamalysin-like metalloproteinases with thrombospondin (TS) motifs (ADAMTS)-5 is the multi-domain metalloproteinase that most potently degrades aggrecan proteoglycan in the cartilage and its activity is implicated in the development of osteoarthritis (OA). To generate specific exosite inhibitors for it, we screened a phage display antibody library in the presence of the zinc-chelating active site-directed inhibitor GM6001 (Ilomastat) and isolated four highly selective inhibitory antibodies. Two antibodies were mapped to react with exosites in the catalytic/disintegrin domains (Cat/Dis) of the enzyme, one in the TS domain and one in the spacer domain (Sp). The antibody reacting with the Sp blocked the enzyme action only when aggrecan or the Escherichia coli-expressed aggrecan core protein were substrates, but not against a peptide substrate. The study with this antibody revealed the importance of the Sp for effective aggrecanolytic activity of ADAMTS-5 and that this domain does not interact with sulfated
... BSD System Managers Manual CAMCONTROL(8) NAME camcontrol -- CAM control program SYNOPSIS camcontrol ,command, [device id] [generic args] [command args] camcontrol devlist [-v] camcontrol periphlist [device id] [-n dev_name] [-u unit_number] camcontrol tur [device id] [generic args] camcontrol inquiry [device id] [generic args] [-D] [-S] [-R] camcontrol start [device id] [generic args] camcontrol stop [device id] [generic args] camcontrol load [device id] [generic args] camcontrol eject [device id] [generic args] camcontrol rescan ,all , bus[:target:lun], camcontrol reset ,all , bus[:target:lun], camcontrol defects [device id] [generic args] ,-f format, [-P] [-G] camcontrol modepage [device id] [generic args] ,-m page , -l, [-P pgctl] [-b , -e] [-d] camcontrol cmd [device id] [generic args] ,-c cmd [args], [-i len fmt] [-o len fmt [args]] camcontrol debug [-I] [-P] [-T] [-S] [-X] [-c] ,all,off,bus[:target[:lun]], camcontrol tags [device id] [generic args] [-N tags] [-q] [-v] ...
Wang L, Pawlak E, Johnson PJ, Belknap JK, Alfandari D, Black SJ. Effects of cleavage by a disintegrin and metalloproteinase with thrombospondin motifs-4 on gene expression and protein content of versican and aggrecan in the digital laminae of horses with starch gruel-induced laminitis. Am J Vet Res. 2012 Jul; 73(7):1047-56 ...
This module implements {{listen}}. local mFileLink = require(Módulo:Link de arquivo) local mTableTools = require(Módulo:TableTools) local mSideBox = require(Módulo:Caixa lateral) local p = {} local hasMidi, hasMissing -- Trackers for categories function p.main(frame) local origArgs = frame:getParent().args local args = {} for k, v in pairs(origArgs) do v = v:match(^%s*(.-)%s*$) if v ~= then args[k] = v end end return p._main(args) end function p._main(args) -- Organise the arguments by number. local numArgs = {} do local origNumArgs = mTableTools.numData(args) origNumArgs[1] = origNumArgs.other -- Overwrite args.filename1 etc. with args.filename etc. origNumArgs = mTableTools.compressSparseArray(origNumArgs) for i, t in ipairs(origNumArgs) do -- Check if the files exist. local obj = (t.filename and mw.title.new(Media: .. t.filename) or t.arquivo and mw.title.new(Media: .. t.arquivo) or t.ficheiro and mw.title.new(Media: .. t.ficheiro)) if obj and obj.exists then ...
swig_c++.tcl # Provides a simple object oriented interface using # SWIGs low level interface. # proc new {objectType handle_r args} { # Creates a new SWIG object of the given type, # returning a handle in the variable "handle_r". # # Also creates a procedure for the object and a trace on # the handle variable that deletes the object when the # handle variable is overwritten or unset upvar $handle_r handle # # Create the new object # eval set handle \[new_$objectType $args\] # # Set up the object procedure # proc $handle {cmd args} "eval ${objectType}_\$cmd $handle \$args" # # And the trace ... # uplevel trace variable $handle_r uw "{deleteObject $objectType $handle}" # # Return the handle so that new can be used as an argument to a procedure # return $handle } proc deleteObject {objectType handle name element op} { # # Check that the object handle has a reasonable form # if {![regexp {_[0-9a-f]*_(.+)_p} $handle]} { error "deleteObject: not a valid object handle: $handle" } # # Remove the ...
I got args halfway into my season and i only got to ride them five times or so, since i do not have a place in the mountains i feel like this may be just to muc
File lib/spec/example/shared_example_group.rb, line 49 def initialize(*args, &example_group_block) set_description(*args) @example_group_block = example_group_block end ...
File lib/spec/example/example_group_factory.rb, line 23 def create_shared_example_group(*args, &example_group_block) # :nodoc: ::Spec::Example::SharedExampleGroup.register(*args, &example_group_block) end ...
11:05:18] ,Darke, So far every file has been named by the primary class inside it, so Args.cc,h has a class Args { definition, etc. The problem is what to do with files that lack class? Ive got the holdover from exult common_types.h, now logically I should rename it to CommonTypes.hpp, just because its the same capitalisation as the other files. But it doesnt contain a CommonTypes class ...
Historically, PHP allows calling functions with fewer actual parameters than required by the function definition. These "non-passed" arguments lead to warning emission and continuation of function execution with uninitialized arguments. ...
TY - JOUR. T1 - Characterization and localization of citrullinated proteoglycan aggrecan in human articular cartilage. AU - Glant, Tibor T.. AU - Ocsko, Timea. AU - Markovics, Adrienn. AU - Szekanecz, Z.. AU - Katz, Robert S.. AU - Rauch, Tibor A.. AU - Mikecz, Katalin. PY - 2016/3/1. Y1 - 2016/3/1. N2 - Background: Rheumatoid arthritis (RA) is an autoimmune disease of the synovial joints. The autoimmune character of RA is underscored by prominent production of autoantibodies such as those against IgG (rheumatoid factor), and a broad array of joint tissue-specific and other endogenous citrullinated proteins. Anti-citrullinated protein antibodies (ACPA) can be detected in the sera and synovial fluids of RA patients and ACPA seropositivity is one of the diagnostic criteria of RA. Studies have demonstrated that RA T cells respond to citrullinated peptides (epitopes) of proteoglycan (PG) aggrecan, which is one of the most abundant macromolecules of articular cartilage. However, it is not known if ...
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src/Elasticsearch/Query/QueryBuilder.php public function addFilter(\Elastica\Query\AbstractQuery $filter) public function addPostFilter(\Elastica\Query\AbstractQuery $postFilter) ./src/Elasticsearch/Provider/Visibility/StrategyInterface.php public function elasticAddFilters(User $user, \Elastica\Query\BoolQuery $filter, Visibility $provider); ./clients/base/api/BulkApi.php public function bulkCall(ServiceBase $api, array $args) ./clients/base/api/CalendarEventsApi.php public function updateCalendarEvent(ServiceBase $api, array $args) public function deleteCalendarEvent(ServiceBase $api, array $args) public function updateRecurringCalendarEvent(SugarBean $bean, ServiceBase $api, array &$args) public function deleteRecordAndRecurrences(ServiceBase $api, array $args) public function deleteRecurrences(SugarBean $bean) protected function initializeArgs(array $args, SugarBean $bean = null) protected function adjustStartDate(array &$args) protected function filterOutRecurrenceFields(array $args) ...
El Sistema de Salud de la Universidad de Miami ofrece la atención más avanzada del sur de la Florida. Para más información, llame al 305-243-4000.
Is there a way to have a metaclass similar to our ClasscallMetaclass at all, in Python3?? I just tested, having class Classcall(type): def __call__(cls, *args, **opts): try: classcall = cls.__classcall__ except AttributeError: return type.__call__(cls, *args, **opts) return classcall(cls, *args, **opts) class MyUniqueRepresentation: __metaclass__ = Classcall @staticmethod def __classcall__(cls, *args, **opts): out = super(cls,cls).__new__(cls, *args, **opts) print(classcall got,type(out), cls) cls.__init__(out,*args,**opts) out._reduction = (type(out), args, opts) return out Loading the above into python2, I get ,,, class Foo(MyUniqueRepresentation): ... def __init__(self, a,b): ... self.a = a ... self.b = b ... ,,, A = Foo(A,B) (classcall got, ,class __main__.Foo,, ,class __main__.Foo,) ,,, A._reduction (,class __main__.Foo,, (A, B), {}) But doing the same in python3, it gives ,,, class Foo(MyUniqueRepresentation): ... def __init__(self, a,b): ... self.a = a ... self.b = b ... ...
Aggrecan antibody [7D4] (aggrecan) for ELISA, IHC-Fr, IHC-P, IP, WB. Anti-Aggrecan mAb (GTX31216) is tested in Human, Bovine samples. 100% Ab-Assurance.
from genshi.core import Markup from trac.wiki.macros import WikiMacroBase class HelloWorldMacro(WikiMacroBase): """Simple HelloWorld macro. Note that the name of the class is meaningful: - it must end with "Macro" - what comes before "Macro" ends up being the macro name The documentation of the class (i.e. what youre reading) will become the documentation of the macro, as shown by the !MacroList macro (usually used in the WikiMacros page). """ revision = "$Rev$" url = "$URL$" def expand_macro(self, formatter, name, text, args): """Return some output that will be displayed in the Wiki content. `name` is the actual name of the macro (no surprise, here itll be `HelloWorld`), `text` is the text enclosed in parenthesis at the call of the macro. Note that if there are no parenthesis (like in, e.g. [[HelloWorld]]), then `text` is `None`. `args` are the arguments passed when HelloWorld is called using a `#!HelloWorld` code block. """ return Hello World, text = %s, args = %s % \ ...
from genshi.core import Markup from trac.wiki.macros import WikiMacroBase class HelloWorldMacro(WikiMacroBase): """Simple HelloWorld macro. Note that the name of the class is meaningful: - it must end with "Macro" - what comes before "Macro" ends up being the macro name The documentation of the class (i.e. what youre reading) will become the documentation of the macro, as shown by the !MacroList macro (usually used in the WikiMacros page). """ revision = "$Rev$" url = "$URL$" def expand_macro(self, formatter, name, text, args): """Return some output that will be displayed in the Wiki content. `name` is the actual name of the macro (no surprise, here itll be `HelloWorld`), `text` is the text enclosed in parenthesis at the call of the macro. Note that if there are no parenthesis (like in, e.g. [[HelloWorld]]), then `text` is `None`. `args` are the arguments passed when HelloWorld is called using a `#!HelloWorld` code block. """ return Hello World, text = %s, args = %s % \ ...
mikelove: trying out: alias rhelp="Rscript -e args ,- commandArgs(TRUE); help(args[2], package=args[3], help_type=html); Sys.sleep(5) --args" (5 Aug ...
O esperado é algo assim? [09-Nov-2017 04:51:35 UTC] hook http_request_args: 1510203095 [09-Nov-2017 04:51:46 UTC] hook… 2 months ago. ...
Versican is a large extracellular matrix proteoglycan that is present in a variety of human tissues. It is encoded by the VCAN gene. Versican is a large chondroitin sulfate proteoglycan with an apparent molecular mass of more than 1000kDa. In 1989, Zimmermann and Ruoslahti cloned and sequenced the core protein of fibroblast chondroitin sulfate proteoglycan. They designated it versican in recognition of its versatile modular structure. Versican belongs to the lectican protein family, with aggrecan (abundant in cartilage), brevican and neurocan (nervous system proteoglycans) as other members. Versican is also known as chondroitin sulfate proteoglycan core protein 2 or chondroitin sulfate proteoglycan 2 (CSPG2), and PG-M. These proteoglycans share a homologous globular N-terminal, C-terminal, and glycosaminoglycan (GAG) binding regions. The N-terminal (G1) globular domain consists of Ig-like loop and two link modules, and has Hyaluronan (HA) binding properties. Versican occurs in 5 isoforms : V0, ...
Perineuronal nets (PNNs) are specialized substructures of the neural extracellular matrix (ECM) which envelop the cell soma and proximal neurites of particular sets of neurons with apertures at sites of synaptic contact. Previous studies have shown that PNNs are enriched with chondroitin sulfate proteoglycans (CSPGs) and hyaluronan, however, a complete understanding of their precise molecular composition has been elusive. In addition, identifying which specific PNN components are critical to the formation of this structure has not been demonstrated. Previous work in our laboratory has demonstrated that the CSPG, aggrecan, is a key activity-dependent component of PNNs in vivo. In order to assess the contribution of aggrecan to PNN formation, we utilized cartilage matrix deficiency (cmd) mice, which lack aggrecan. Herein, we utilized an in vitro model, dissociated cortical culture, and an ex vivo model, organotypic slice culture, to specifically investigate the role aggrecan plays in PNN ...
Background: Dysregulation of microRNAs (miRNAs) has been reported to be associated with Intervertebral Disc Degeneration (IDD), and accumulating evide..
Until the late 1970s, hyaluronic acid was described as a "goo" molecule, a ubiquitous carbohydrate polymer that is part of the extracellular matrix.[11] For example, hyaluronic acid is a major component of the synovial fluid, and was found to increase the viscosity of the fluid. Along with lubricin, it is one of the fluids main lubricating components. Hyaluronic acid is an important component of articular cartilage, where it is present as a coat around each cell (chondrocyte). When aggrecan monomers bind to hyaluronan in the presence of HAPLN1 (hyaluronanic acid and proteoglycan link protein 1), large, highly negatively charged aggregates form. These aggregates imbibe water and are responsible for the resilience of cartilage (its resistance to compression). The molecular weight (size) of hyaluronan in cartilage decreases with age, but the amount increases.[12] A lubricating role of hyaluronan in muscular connective tissues to enhance the sliding between adjacent tissue layers has been ...
The 2013 Annual Meeting of the Asia Pacific Chapter of the Tissue Engineering and Regenerative Medicine International Society (TERMIS-AP), Shanghai & Wuzhen, China, 23-26 October 2013. In Conference Program, 2013, p. 241, abstract no. S12-O-1 ...
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In the project root set the following alias $: alias render=mvn exec:java -Dexec.mainClass=Main # Using OPSIN to load porphyrin and generate a PDF $: render -Dexec.args="-name porphyrin -pdf" # Highlight one of the pyrrole in porphyrin $: render -Dexec.args="-name porphyrin -pdf -sma n1cccc1" # Show atom numbers $: render -Dexec.args="-name porphyrin -pdf -atom-numbers" # Show CIP labels $: render -Dexec.args="-name (2R)-butan-2-ol -pdf -cip-labels" # Generate a PDF / SVG for ethanol SMILES $: render -Dexec.args="-smi CCO -pdf ethanol.pdf -svg ethanol.svg" # Load a molfile $: render -Dexec.args="-mol ChEBI_6701.mol -pdf chebi-6701.pdf ...
In the project root set the following alias $: alias render=mvn exec:java -Dexec.mainClass=Main # Using OPSIN to load porphyrin and generate a PDF $: render -Dexec.args="-name porphyrin -pdf" # Highlight one of the pyrrole in porphyrin $: render -Dexec.args="-name porphyrin -pdf -sma n1cccc1" # Show atom numbers $: render -Dexec.args="-name porphyrin -pdf -atom-numbers" # Show CIP labels $: render -Dexec.args="-name (2R)-butan-2-ol -pdf -cip-labels" # Generate a PDF / SVG for ethanol SMILES $: render -Dexec.args="-smi CCO -pdf ethanol.pdf -svg ethanol.svg" # Load a molfile $: render -Dexec.args="-mol ChEBI_6701.mol -pdf chebi-6701.pdf ...
Bassani-Sternberg M, et al. (2016) Direct identification of clinically relevant neoepitopes presented on native human melanoma tissue by mass spectrometry. Nat Commun 7, 13404 ...
Title : seqname Usage : $obj-,seq_id($newval) Function: There are many cases when you make a feature that you do know the sequence name, but do not know its actual sequence. This is an attribute such that you can store the seqname. This attribute should *not* be used in GFF dumping, as that should come from the collection in which the seq feature was found. Returns : value of seqname Args : newvalue (optional ...
Evaluation of serum ARGS neoepitope as an osteoarthritis biomarker using a standardized model for exercise-induced cartilage extra cellular matrix turnover ...
The influence of the presence of certain amino acids at different concentrations on the catabolic activity of the bacteria Desulfotomaculum ruminis was studied. Introduction of amino acids of the simple chain molecule in concentrations up to 10 g/dm3 in the Starkey media leads to a small...
Hyaluronan (HA) is widely detected in biological samples and its concentration is most commonly determined by the use of a labeled specific HA binding protein (aggrecan G1-IGD-G2, HABP), employing membrane blotting and sandwich enzyme-linked immunosorbent assay (ELISA)-like methods. However, the detected signal intensity or the quantified value obtained by using these surface-based methods is related to the molecular mass (M) of HA, especially for HA in the low M range below ~150 kDa. At the same mass or mass concentration, higher M HA gives a higher signal than lower M HA. We have experimentally determined the quantitative relationship between the M of HA (in the range 20-150 kDa) and the relative signal intensity in comparison with a standard HA, in a sandwich ELISA-like assay. An M-dependent signal correction factor (SCF) was calculated and used to correct the signal intensity, so that the corrected concentration value would more accurately reflect the true HA concentration in solution. The ...
STUDY DESIGN: The inflammatory responses of primary human intervertebral disc (IVD) cells to tumor necrosis factor α (TNF-α) and an antagonist were evaluated in vitro. OBJECTIVE: To investigate an ability for soluble TNF ...
"Aggrecans" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject ... This graph shows the total number of publications written about "Aggrecans" by people in this website by year, and whether " ... Below are the most recent publications written about "Aggrecans" by people in Profiles. ...
Aggrecans / metabolism * Animals * Biomarkers / chemistry* * Biomarkers / metabolism * Bone Matrix / chemistry * Bone and Bones ...
Aggrecans / metabolism * Animals * Bone Marrow Transplantation / methods* * Collagen Type II / metabolism * Disease Models, ...
Study Cartilage Bones Joints flashcards from Jason Hsu
0 (Aggrecans); 0 (RNA, Messenger); EC 4.2.2.20 (Chondroitin ABC Lyase). [Em] M s de entrada:. 1712. ...
Proteases capable of degrading aggrecans are called aggrecanases, and they are members of the ADAM (A Disintegrin And ... Nap RJ, Szleifer I (November 2008). "Structure and interactions of aggrecans: statistical thermodynamic approach". Biophys. J. ...
Aggrecans (Aggrecan) 3. Messenger RNA (mRNA) 4. Complementary DNA (cDNA) 5. Versicans (Versican) ...
... chondroitin sulfate refers to a group of glycosaminoglycans called aggrecans. Aggrecans aggregate to form the cartilage. ... Aggrecans such as chondroitin bind to hyaluronic acid to form superstructures of negatively charged aggregates. These ...
Aggrecanases act on large proteoglycans known as aggrecans, which are components of connective tissues such as cartilage. The ...
It cleaves both type II collagen and aggrecans. "Cathepsin K is clearly upregulated in joints in early osteoarthritis," she ...
... aggrecans and collagens. COMP is a pentameric glycoprotein of the cartilage extracellular matrix that is localized ...
TY - JOUR. T1 - In vitro chondrogenesis of bone marrow-derived mesenchymal stem cells in a photopolymerizing hydrogel. AU - Williams, Christopher G.. AU - Kim, Tae Kyun. AU - Taboas, Anya. AU - Malik, Athar. AU - Manson, Paul. AU - Elisseeff, Jennifer Hartt. PY - 2003/8. Y1 - 2003/8. N2 - Mesenchymal stem cells (MSCS) from skeletally mature goats were encapsulated in a photopolymerizing poly(ethylene glycol)-based hydrogel and cultured with or without transforming growth factor β1 (TGF) to study the potential for chondrogenesis in a hydrogel scaffold system amenable to minimally invasive implantation. Chondrogenic differentiation was evaluated by histological, biochemical, and RNA analyses for the expression of cartilage extracellular matrix components. The two control groups studied were MSCs cultured in monolayer and MSCs encapsulated in the hydrogel and cultured for 6 weeks in chondrogenic medium without TGF-β1 (6wk-TGF). The three experimental time points for encapsulated cells studied ...
102000016284 Aggrecans Human genes 0 description 2 * 210000001188 Cartilage, Articular Anatomy 0 description 2 ...
Expression of alternatively spliced epidermal growth factor-like domains in aggrecans of different species. Evidence for a ...
Recent studies support the promise of using lubricin as a therapy for OA.6 Aggrecans are formed by a core protein which is ... Other proteins include noncollagenous, aggrecans, leucine-rich repeated, structural, regulatory, and others (Table 1, Figures 1 ... MMP-13 being the most involved in cleaving type II collagen and aggrecans and MMP-7 as an early predictor of OA, as much as 10 ... 47 It has been proven that IL-10 is involved in stimulating the synthesis of type II collagen and aggrecans and decreases TNFα ...
The increased in cartilage volume is explained by chondrocyte proliferation, increased expression of cartilage aggrecans, Type ... 6] Theses mechanosensitive genes includes collagens, aggrecans, growth proteins, interleukins (IL) 1, 4, and 6, matrix ...
TY - JOUR. T1 - A hyaluronic acid binding peptide-polymer system for treating osteoarthritis. AU - Faust, Heather J.. AU - Sommerfeld, Sven D.. AU - Rathod, Sona. AU - Rittenbach, Andrew. AU - Ray, Sangeeta. AU - Tsui, Benjamin. AU - Pomper, Martin Gilbert. AU - Amzel, Mario L. AU - Singh, Anirudha. AU - Elisseeff, Jennifer Hartt. PY - 2018/11/1. Y1 - 2018/11/1. N2 - Hyaluronic acid (HA) is found naturally in synovial fluid and is utilized therapeutically to treat osteoarthritis (OA). Here, we employed a peptide-polymer cartilage coating platform to localize HA to the cartilage surface for the purpose of treating post traumatic osteoarthritis. The objective of this study was to increase efficacy of the peptide-polymer platform in reducing OA progression in a mouse model of post-traumatic OA without exogenous HA supplementation. The peptide-polymer is composed of an HA-binding peptide (HABP) conjugated to a heterobifunctional poly (ethylene glycol) (PEG) chain and a collagen binding peptide ...
In cartilage, ECM forms a major fraction of the tissue, type II collagen and aggrecans being the most abundant macromolecules. ...
However, recent research has demonstrated that some adhesive forces in one of those compounds (aggrecans) may be significant ... In particular, the tendency of the aggrecans to stick together under compressive force, then come apart moments after ...
1996). Variations in the chondroitin sulfate-protein linkage region of aggrecans from bovine nasal and human articular ...
TY - JOUR. T1 - Static compression of single chondrocytes catabolically modifies single-cell gene Expression. AU - Leipzig, Nic D.. AU - Athanasiou, Kyriacos A.. PY - 2008/3/15. Y1 - 2008/3/15. N2 - Previous work has established that mechanical forces can lead to quantifiable alterations in cell function. However, how forces change gene expression in a single cell and the mechanisms of force transmission to the nucleus are poorly understood. Here we demonstrate that the gene expression of proteins related to the extracellular matrix in single articular chondrocytes is modified by compressive forces in a dosage-dependent manner. Increasing force exposure catabolically shifts single-cell mRNA levels of aggrecan, collagen IIa, and tissue inhibitor of metalloproteinase-1. Cytohistochemistry reveals that the majority of strain experienced by the cell is also experienced by the nucleus, resulting in considerable changes in nuclear volume and structure. Transforming growth factor-β1 and insulin-like ...
HA plays the key role in immobilizing aggrecans in articular cartilage; this balances the tension and compressive resilience in ... HA decreases the molecular size of the cartilage matrix and increases its proportion to the aggrecans by age-related change [25 ...
Aggrecans, metabolism, Animals, Cartilage, Articular, cytology, metabolism, Chondrocytes, drug effects, metabolism, ...
These structural units, called aggrecans, are important for the elasticity, resilience and shock-absorbing properties of ...
These structural units, called aggrecans, are important for the elasticity, resilience and shock-absorbing properties of ...
  • It cleaves both type II collagen and aggrecans. (the-rheumatologist.org)
  • We have arbitrarily divided the ECM into two sections: (1) The collagen: PG complex itself, including the external membrane-bound PG, (e.g., aggrecans and decorin) and (2) The perturbations caused by the effects of the surrounding internal and external environment. (omicsonline.org)
  • Aggrecans" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (umassmed.edu)
  • Proteases capable of degrading aggrecans are called aggrecanases, and they are members of the ADAM (A Disintegrin And Metalloprotease) protein family. (wikipedia.org)
  • En plus, cette dernière a montré de différents effets sur plusieurs voies de signalisation stimulées par l'IL-1β et le TNF-α telles que les voies des MAPKs (Mitogen activated protein kinase), de l'AKT, et de la p70 S6 kinase. (umontreal.ca)
  • La Rapamycine a inhibé partiellement l'activation de la phosphorylation de l'ERK1/2 MAPK (extracellular signal-regulated protein kinase MAPK) en présence du TNF-α seulement. (umontreal.ca)
  • After a stimulus to your tendon, the cells may change their activity to secrete a water-attracting-protein called Aggrecans. (otpbooks.com)
  • After activation with recombinant human stromelysin and trypsin, both collagenases were able to degrade human and porcine aggrecans to a similar extent. (uni-bielefeld.de)
  • This graph shows the total number of publications written about "Aggrecans" by people in this website by year, and whether "Aggrecans" was a major or minor topic of these publications. (umassmed.edu)
  • In particular, the tendency of the aggrecans to stick together under compressive force, then come apart moments after represents a major compression-dissipation system. (asknature.org)