Aggrecans: Large HYALURONAN-containing proteoglycans found in articular cartilage (CARTILAGE, ARTICULAR). They form into aggregates that provide tissues with the capacity to resist high compressive and tensile forces.Hyalin: A clear, homogenous, structureless, eosinophilic substance occurring in pathological degeneration of tissues.Keratan Sulfate: A sulfated mucopolysaccharide initially isolated from bovine cornea. At least two types are known. Type I, found mostly in the cornea, contains D-galactose and D-glucosamine-6-O-sulfate as the repeating unit; type II, found in skeletal tissues, contains D-galactose and D-galactosamine-6-O-sulfate as the repeating unit.Extracellular Matrix Proteins: Macromolecular organic compounds that contain carbon, hydrogen, oxygen, nitrogen, and usually, sulfur. These macromolecules (proteins) form an intricate meshwork in which cells are embedded to construct tissues. Variations in the relative types of macromolecules and their organization determine the type of extracellular matrix, each adapted to the functional requirements of the tissue. The two main classes of macromolecules that form the extracellular matrix are: glycosaminoglycans, usually linked to proteins (proteoglycans), and fibrous proteins (e.g., COLLAGEN; ELASTIN; FIBRONECTINS; and LAMININ).Lectins, C-Type: A class of animal lectins that bind to carbohydrate in a calcium-dependent manner. They share a common carbohydrate-binding domain that is structurally distinct from other classes of lectins.Chondroitin Sulfates: Derivatives of chondroitin which have a sulfate moiety esterified to the galactosamine moiety of chondroitin. Chondroitin sulfate A, or chondroitin 4-sulfate, and chondroitin sulfate C, or chondroitin 6-sulfate, have the sulfate esterified in the 4- and 6-positions, respectively. Chondroitin sulfate B (beta heparin; DERMATAN SULFATE) is a misnomer and this compound is not a true chondroitin sulfate.Proteoglycans: Glycoproteins which have a very high polysaccharide content.Cartilage, Articular: A protective layer of firm, flexible cartilage over the articulating ends of bones. It provides a smooth surface for joint movement, protecting the ends of long bones from wear at points of contact.

Association of the aggrecan keratan sulfate-rich region with collagen in bovine articular cartilage. (1/821)

Aggrecan, the predominant large proteoglycan of cartilage, is a multidomain macromolecule with each domain contributing specific functional properties. One of the domains contains the majority of the keratan sulfate (KS) chain substituents and a protein segment with a proline-rich hexapeptide repeat sequence. The function of this domain is unknown but the primary structure suggests a potential for binding to collagen fibrils. We have examined binding of aggrecan fragments encompassing the KS-rich region in a solid-phase assay. A moderate affinity (apparent Kd = 1.1 microM) for isolated collagen II, as well as collagen I, was demonstrated. Enzymatic digestion of the KS chains did not alter the capacity of the peptide to bind to collagen, whereas cleavage of the protein core abolished the interaction. The distribution of the aggrecan KS-rich region in bovine tarsometatarsal joint cartilage was investigated using immunoelectron microscopy. Immunoreactivity was relatively low in the superficial zone and higher in the intermediate and deep zones of the uncalcified cartilage. Within the pericellular and territorial matrix compartments the epitopes representing the aggrecan KS-rich region were detected preferentially near or at collagen fibrils. Along the fibrils, epitope reactivity was non-randomly distributed, showing preference for the gap region within the D-period. Our data suggest that collagen fibrils interact with the KS-rich regions of several aggrecan monomers aligned within a proteoglycan aggregate. The fibril could therefore serve as a backbone in at least some of the aggrecan complexes.  (+info)

Fatty acids modulate the composition of extracellular matrix in cultured human arterial smooth muscle cells by altering the expression of genes for proteoglycan core proteins. (2/821)

In diabetes-associated microangiopathies and atherosclerosis, there are alterations of the extracellular matrix (ECM) in the intima of small and large arteries. High levels of circulating nonesterified fatty acids (NEFAs) are present in insulin resistance and type 2 diabetes. High concentrations of NEFAs might alter the basement membrane composition of endothelial cells. In arteries, smooth muscle cells (SMCs) are the major producers of proteoglycans and glycoproteins in the intima, and this is the site of lipoprotein deposition and modification, key events in atherogenesis. We found that exposure of human arterial SMCs to 100-300 micromol/albumin-bound linoleic acid lowered their proliferation rate and altered cell morphology. SMCs expressed 2-10 times more mRNA for the core proteins of the proteoglycans versican, decorin, and syndecan 4 compared with control cells. There was no change in expression of fibronectin and perlecan. The decorin glycosaminoglycan chains increased in size after exposure to linoleic acid. The ECM produced by cells grown in the presence of linoleic acid bound 125I-labeled LDL more tightly than that of control cells. Darglitazone, a peroxisome proliferator-activated receptor (PPAR)-gamma ligand, neutralized the NEFA-mediated induction of the decorin gene. This suggests that some of the NEFA effects are mediated by PPAR-gamma. These actions of NEFAs, if present in vivo, could contribute to changes of the matrix of the arterial intima associated with micro- and macroangiopathies.  (+info)

Immune responses to cartilage link protein and the G1 domain of proteoglycan aggrecan in patients with osteoarthritis. (3/821)

OBJECTIVE: To determine whether patients with osteoarthritis (OA) express cellular immunity to cartilage link protein (LP) and the G1 globular domain of proteoglycan (PG) aggrecan, and whether immunity to the G1 domain is influenced by the removal of keratan sulfate (KS). METHODS: LP and the G1 globular domain of PG were isolated from human and/or bovine cartilage and used in proliferation assays with peripheral blood lymphocytes (PBL) from 42 patients with OA and 40 healthy control subjects. RESULTS: Patients with OA expressed a higher prevalence of cellular immunity to human cartilage LP (42.4%) compared with the control group (13.3%). The prevalence of immune reactivity to bovine LP in patients with OA was lower (35.7%) compared with the immunity to human LP, but remained similar in the control group (13.8%). PBL from patients with OA exhibited low reactivity to the native G1 domain of bovine PG. However, removal of KS chains from the G1 globular domain resulted in increased cellular immune responses to the G1 domain in OA patients (45.8%) compared with the control group (7.7%). CONCLUSION: These results indicate the presence of immunity to cartilage-derived LP and the G1 globular domain of PG aggrecan in patients with OA and the inhibitory effect of KS chains on the G1 domain on the expression of this immunity in OA patients. This immune reactivity is commonly observed in patients with inflammatory joint disease and can experimentally induce arthritis. Thus, it may be involved in the pathogenesis of OA.  (+info)

Changes in joint cartilage aggrecan after knee injury and in osteoarthritis. (4/821)

OBJECTIVE: To determine the concentrations of aggrecan fragments in synovial fluid from patients with knee joint injury, osteoarthritis (OA), or acute pyrophosphate arthritis (PPA; pseudogout), and to test their relative reactivity with the 846 epitope, a putative marker of cartilage aggrecan synthesis. METHODS: Samples of knee joint fluid from 385 patients and 9 healthy-knee volunteers were obtained in a cross-sectional study. Study groups were acute PPA/ pseudogout (n = 60), anterior cruciate ligament (ACL) rupture (n = 159), meniscus lesion (n = 129), and primary knee OA (n = 37). The 846 epitope on aggrecan was assayed by competitive solution-phase radioimmunoassay. Aggrecan fragments were assayed by enzyme-linked immunosorbent assay using a monoclonal antibody (1-F21). Cartilage oligomeric matrix protein (COMP), C-propeptide of type II collagen (CPII), bone sialoprotein, matrix metalloproteinases 1 and 3, and tissue inhibitor of metalloproteinases 1 were previously quantified by immunoassays. RESULTS: Reactivity of the 846 epitope was increased in all study groups compared with the reference group, and was highest in patients with primary OA. The median levels (in microg fetal aggrecan equivalents/ml) of the epitope were 0.28 (range 0.24-0.47) in the reference group, 0.48 (range 0.26-1.32) in PPA/pseudogout, 0.61 (range 0.12-2.87) in ACL rupture, 0.53 (range 0.22-3.02) in meniscus lesion, and 0.68 (range 0.31-4.31) in primary OA. The 846 epitope reactivity per microg aggrecan fragments in the joint fluid was higher in late-stage OA than in early-stage OA. Epitope 846 reactivity correlated positively with several markers of matrix turnover, particularly with COMP (r(s) = 0.421) and CPII (r(s) = 0.307). CONCLUSION: The observed differences in 846 epitope reactivity in synovial fluid, and its concentration in relation to aggrecan and other markers of matrix turnover, were consistent with marked ongoing changes in aggrecan turnover after joint injury and in the development of OA. OA is thus a disease characterized by dynamic changes in tissue macromolecule turnover, which is reflected by measurable changes in aggrecan epitopes in the synovial fluid.  (+info)

Longitudinal and cross-sectional variability in markers of joint metabolism in patients with knee pain and articular cartilage abnormalities. (5/821)

OBJECTIVE: To determine the within- and between-patient variability in the concentrations of synovial fluid, serum and urine markers of joint tissue metabolism in a cohort of patients with knee pain and cartilage changes consistent with early-stage knee osteoarthritis. DESIGN: Samples of synovial fluid, serum, and urine were obtained from 52 patients on eight different occasions during 1 year, as part of a clinical trial in patients with cartilage abnormalities and knee pain. In joint fluid, aggrecan fragments were quantified by dye precipitation and enzyme-linked immunosorbent assay (ELISA), and matrix metalloproteinases-1 and -3, and tissue inhibitor of metalloproteinases-1 by sandwich ELISAs. In serum, keratan sulfate was quantified by ELISA. Type I collagen N-telopeptide cross-links in urine were determined by ELISA. RESULTS: The degree of cross-sectional variability in marker concentrations did not vary between the different sampling occasions, and did not differ between the periods of weeks 0 (baseline), 1-4 (treatment) and 13-26 (follow-up). Both between-patient and within-patient coefficients of variation varied for markers in different body fluid compartments, with the lowest variability for serum keratan sulfate, followed by urine type I collagen N-telopeptide crosslinks, and the highest for synovial fluid markers. For synovial fluid, aggrecan fragments showed the least variability, and matrix metalloproteinases the highest. One patient with septic arthritis showed a fivefold peak increase in joint fluid aggrecan fragment concentrations, while the concentration of matrix metalloproteinase-3 increased 100-fold. CONCLUSIONS: Molecular markers of joint tissue metabolism have been suggested as, for example, outcome measures for clinical trials of disease-modifying drugs in osteoarthritis. This report is the first to present data on between- and within-patient variability for such molecular markers in three different body fluid compartments in stable cohort of patients. The availability of such data enables calculations to determine the number of patients needed in prospective studies using these markers as outcome measures.  (+info)

Resistance of small leucine-rich repeat proteoglycans to proteolytic degradation during interleukin-1-stimulated cartilage catabolism. (6/821)

A bovine nasal-cartilage culture system has been utilized to analyse the catabolic events occurring in response to interleukin-1beta over a 14-day period. An early event following the start of interleukin-1 treatment was the release of glycosaminoglycan into the culture medium. This release was accompanied by the appearance in the tissue, and shortly thereafter also in the culture media, of a globular domain (G1)-containing aggrecan degradation product generated by the action of aggrecanase. Link protein was also released from the cartilage with a similar timeframe to that of the G1 fragment, although there was no evidence of its proteolytic degradation. By comparison with aggrecan, the small leucine-rich repeat proteoglycans decorin, biglycan and lumican showed a resistance to both proteolytic cleavage and release throughout the culture period. In contrast, fibromodulin exhibited a marked decrease in size after day 4, presumably due to proteolytic modification, but the major degradation product was retained throughout the culture period. Also in contrast with the early changes in the components of the proteoglycan aggregate, type II collagen did not display signs of extensive degradation until much later in the culture period. Collagen degradation products compatible with collagenase action first appeared in the medium by day 10 and increased thereafter. These data demonstrate that the leucine-rich repeat proteoglycans are resistant to proteolytic action during interleukin-1-stimulated cartilage catabolism, compared with aggrecan. This resistance and continued interaction with the surface of the collagen fibrils may help to stabilize the collagen fibrillar network and protect it from extensive proteolytic attack during the early phases of cartilage degeneration.  (+info)

Alcohol promotes in vitro chondrogenesis in embryonic facial mesenchyme. (7/821)

Ethanol is a well-recognized teratogen in vertebrates that can perturb the development of the facial primordia and various other embryonic structures. However,the mechanisms underlying alcohol's effects on embryogenesis are currently unclear. Recent evidence suggests that the cranial neural crest, which forms the entire facial skeleton, may be a particularly sensitive target of ethanol teratogenicity. In the present study we have examined the influence of in vitro ethanol exposure on cartilage differentiation in micromass cultures of mesenchymal cells isolated from the various facial primordia (maxillary, mandibular, frontonasal, and hyoid processes) of the stage 24 chick embryo. In all four populations of facial mesenchyme, exposure to 1-1.5% ethanol promoted marked increases in Alcian blue-positive cartilage matrix formation, a rise in 35SO4 accumulation into matrix glycosaminoglycans, and enhanced expression of cartilage-characteristic type II collagen and aggrecan gene transcripts. In frontonasal and mandibular mesenchyme cultures, which undergo extensive spontaneous cartilage formation, ethanol treatment quantitatively elevated both matrix production and cartilage-specific gene transcript expression. In cultures of maxillary process and hyoid arch mesenchyme, which form little or no cartilage spontaneously, ethanol exposure induced the formation of chondrogenic cell aggregates and the appearance of aggrecan and type II collagen mRNAs. These actions were not restricted to ethanol, since tertiary butanol treatment also enhanced cartilage differentiation in facial mesenchyme cultures. Our findings demonstrate a potent stimulatory effect of alcohol on the differentiation of prechondrogenic mesenchyme of the facial primordia. Further analysis of this phenomenon might yield insight into the developmental mechanisms underlying the facial dysmorphologies associated with embryonic ethanol exposure.  (+info)

The early molecular natural history of experimental osteoarthritis. I. Progressive discoordinate expression of aggrecan and type II procollagen messenger RNA in the articular cartilage of adult animals. (8/821)

OBJECTIVE: To quantify changes in the chondrocyte metabolism of aggrecan core protein and type II procollagen messenger RNA (mRNA) during the early and middle phases of experimental osteoarthritis (OA) in animals. METHODS: Experimental OA was induced by transecting the cranial cruciate ligament of the stifle joint in adult animals; articular cartilage was harvested and analyzed after 4, 10, and 32 weeks. RESULTS: Northern blot analysis revealed no change in aggrecan mRNA 4 weeks after surgery compared with aggrecan mRNA in the unoperated contralateral control joints; aggrecan mRNA levels became significantly elevated by 10 and 32 weeks after surgery. In OA cartilage, type II procollagen mRNA was dramatically and progressively elevated at all times after surgery. The relative increases in type II procollagen mRNA exceeded the relative increases in aggrecan mRNA at all times after surgery, and these differences increased progressively over time. Articular chondrocytes became activated globally (total RNA increases) and specifically (mRNA increase) early after joint injury and remained activated throughout the early and middle phases of this experimental OA. CONCLUSION: The early natural history of experimental OA is characterized by a progressive imbalance in the mRNA expression of aggrecan and type II procollagen in articular chondrocytes. These results suggest that the stimuli for the transcription of these 2 genes are fundamentally different in this animal model.  (+info)

*Aggrecan

Proteases capable of degrading aggrecans are called aggrecanases, and they are members of the ADAM (A Disintegrin And ... Nap RJ, Szleifer I (November 2008). "Structure and interactions of aggrecans: statistical thermodynamic approach". Biophys. J. ...

*Aggrecanase

... s act on large proteoglycans known as aggrecans, which are components of connective tissues such as cartilage. The ...
Aggrecan loss from cartilage in arthritis is mediated by aggrecanases. Aggrecanases cleave aggrecan preferentially in the chondroitin sulfate-2 (CS-2) domain and secondarily at the E373↓374A bond in the interglobular domain (IGD). However, IGD cleavage may be more deleterious for cartilage biomechanics because it releases the entire CS-containing portion of aggrecan. Recent studies identifying aggrecanase-2 (ADAMTS-5) as the predominant aggrecanase in mouse cartilage have not distinguished aggrecanolysis in the IGD from aggrecanolysis in the CS-2 domain. We generated aggrecan knockin mice with a mutation that rendered only the IGD resistant to aggrecanases in order to assess the contribution of this specific cleavage to cartilage pathology. The knockin mice were viable and fertile. Aggrecanase cleavage in the aggrecan IGD was not detected in knockin mouse cartilage in situ nor following digestion with ADAMTS-5 or treatment of cartilage explant cultures with IL-1α. Blocking cleavage in the IGD ...
Rabbit Polyclonal Anti-Aggrecan Neoepitope Antibody [DyLight 650]. Validated: WB, Flow, ICC/IF, IHC-Fr, IHC-P. Tested Reactivity: Human, Mouse, Rat, and more. 100% Guaranteed.
In this thesis, the shear and self-adhesion nanomechanical properties between opposing cartilage aggrecan macromolecules were probed. In addition, nanoscale dynamic oscillatory mechanical properties of cartilage and its type II collagen network was measured. Aggrecan shear nanomechanics was assessed via microcontact printing and lateral force microscopy. Lateral force between aggrecan and the probe tip, and compression of aggrecan was simultaneously measured in 0.001 - 1.0 M NaCl aqueous solutions. Using the microsized tip (Rtip ~ 2.5 [mu]m) enabled a large assembly of ~ 103 aggrecan molecules to interact simultaneously, closely mimicking the in vivo conditions.Both electrostatic and nonelectrostatic components were identified to importantly contribute to aggrecan shear. At near physiological IS (0.1 M), significant rate dependence was observed, suggestive of visco/poroelastic interactions within the aggrecan layer. By using an aggrecan end-functionalized colloidal tip, shear of two opposing ...
Buy our Recombinant Human Aggrecan protein. Ab114254 is a full length protein produced in Wheat germ and has been validated in WB, ELISA, SDS-PAGE. Abcam…
OBJECTIVE: To validate a modified ligand-binding assay for the detection of aggrecanase generated aggrecan fragments with the ARGS neoepitope in synovial fluid (SF) and blood, and to verify the identity of aggrecan fragments found in blood. DESIGN: An enzyme-linked immunosorbent assay (ELISA) on the Meso Scale Discovery (MSD) platform for detection of ARGS-aggrecan was validated, using a standard made from recombinant human aggrecan. Matched samples of SF, serum, plasma, and urine were obtained from 36 subjects at different time points after knee injury, and analysed for ARGS-aggrecan content. Aggrecan was purified from serum and plasma pools and analysed by Western blot. RESULTS: The limits of quantification for the ARGS-aggrecan assay was between 0.2 and 0.025 pmol ARGS/ml, and the sensitivity of the assay was improved two-fold compared to when using a standard purified from human donors. The ARGS concentrations were highest in SF (mean, range; 3.02, 0.36-30.22 pmol/ml), 20 times lower in the ...
Articular cartilage consists of a relatively small number of cells and an abundant ECM. The major components of the ECM are collagen fibrils and aggregating proteoglycan aggrecan. Collagen fibrils, mainly type II collagen together with minor types IX and XI, form a meshwork that provides the tensile strength of the tissue. Aggrecan forms a large aggregated complex interacting with hyaluronan via link proteins and fills the interstitium of the collagen meshwork. Aggrecan provides a hydrated gel that gives cartilage its ability to withstand compression.. In normal cartilage, the turnover and synthesis of ECM macromolecules is at equilibrium, but in rheumatoid arthritis (RA) and osteoarthritis (OA) the loss of ECM components exceeds new synthesis. The primary cause of this imbalance is elevated activity of the proteinase that degrades aggrecan and collagen. Aggrecan loss initially occurs most markedly just beneath the joint surface, which is followed by mechanical failure of the tissue and collagen ...
Dipen is a medicine available in a number of countries worldwide. A list of US medications equivalent to Dipen is available on the Drugs.com website.
We use cookies to ensure that we give you the best experience on our website. If you click Continue well assume that you are happy to receive all cookies and you wont see this message again. Click Find out more for information on how to change your cookie settings ...
cont.) A combination of AFM, biochemical, and polymer statistical methodologies was used to better understand the dependence of aggrecan structure and stiffness on the properties of its constituent GAG chains. The fetal epiphyseal aggrecan had a denser GAG brush region and longer GAG chains, which correlated with a higher effective persistence length of fetal core protein compared to that of mature nasal aggrecan. The effect of increasing the concentration of aggrecan on the substrate resulted in a decrease in molecular extension, suggesting a flexible protein core backbone, which allowed aggrecan to entangle and interact with neighboring molecules. AFM imaging of the conformation of aggrecan that had been deposited on substrates from solutions of varying ionic strength (IS), from DI water to the hysiological IS of 0.1 M NaCl, allowed for direct visualization of the collapse of the molecule on the substrate at the highest IS, due to charge shielding of the CS-GAGs by by Na+ counter-ions. Lastly, ...
Product Rat Aggrecan (AGC) ELISA Kit From DLdevelop - An ELISA kit based on the sandwich method for detection and quantification of Rat Aggrecan (AGC)
Gentaur molecular products has all kinds of products like :search , Kamiya \ Aggrecan Clone 3C7 \ MC-1003 for more molecular products just contact us
... the G1 domain (the N-terminal globular domain of aggrecan) and are C-terminally truncated by proteolysis at a number of sites. of mature bovine articular cartilage and establish the presence of a novel proteolytic pathway for aggrecanolysis in the cells and/or matrix of mature articular cartilages. EXPERIMENTAL Materials Porcine kidney m-calpain was purchased from Calbiochem. Chondroitinase ABC, endo-galactosidase and keratanase II were obtained from Seikagaku America (East Falmouth, MA, U.S.A.). Goat anti-mouse secondary antibody and mouse mAb isotyping kit were from Amersham Biosciences (Little Chalfont, Amersham, Bucks., U.K.). The affinity column HiTrap? Protein A HP and Sepharose CL-2B were from Amersham Biosciences (Uppsala, Sweden). Preparation of mAb SK-28 The antigen used for immunization was the ovalbumin-linked peptide aggrecan cleavages by m-calpain The Western-blot data (Figures ?(Figures1A,1A, ...
The work presented here provides the first direct evidence that m-calpain, or a proteinase that shares the same substrate specificity, is responsible for the generation of a major proportion (perhaps 40%) of the C-terminally truncated species of aggrecan present in the extracellular matrix of mature articular cartilages in vivo. It has been clearly shown that m-calpain can be readily detected by enzymic activity and immunological characterization in chondrocyte cultures, cartilage extracts and synovial fluids [33,35], and its abundance has been shown to correlate with the severity of arthritis in both animal models [31,32] and human disease [34]. The mAb SK-28, which is described for the first time in the present paper, has been used here to investigate the specific role and natural substrate of m-calpain in chondrocytes or normal cartilage matrix in situ. The SK-28 antibody, which reacts with a neoepitope generated by m-calpain-mediated cleavage of bovine aggrecan at Ala719-Ala720, detects two ...
The proteoglycan aggrecan is an important major component of cartilage matrix that gives articular cartilage the ability to withstand compression. Increased breakdown of aggrecan is associated with the development of arthritis and is considered to be catalyzed by aggrecanases, members of the ADAM-TS family of metalloproteinases. Four endogenous tissue inhibitors of metalloproteinases (TIMPs) regulate the activities of functional matrix metalloproteinases (MMPs), enzymes that degrade most components of connective tissue, but no endogenous factors responsible for the regulation of aggrecanases have been found. We show here that the N-terminal inhibitory domain of TIMP-3, a member of the TIMP family that has functional properties distinct from other TIMPs, is a strong inhibitor of human aggrecanases 1 and 2, with K(i) values in the subnanomolar range. This truncated inhibitor, which lacks the C-terminal domain that is responsible for interactions with molecules other than active metalloproteinases, is
Surgery McGill University Montreal Canada Our previous work showed that the cartilage proteoglycan aggrecan could induce an erosive polyarthritis and spondylitis in BALB c mice and the G1 globular domain of the aggrecan G1 contained the arthritogenic region To elucidate whether autoreactive T cells to G1 are expressed in rheumatoid arthritis patients we analyzed the frequency of human G1 specific T cells in the peripheral blood of five rheumatoid arthritis patients and tried to establish G1 reactive T cell lines from these rheumatoid arthritis patients The results showed that the G1 specific T cells in PBL were detectable at the range of 4 97 0 5 10 6 in peripheral blood lymphocytes We have also generated 15 G1 specific T lymphocyte lines from these pateints with a standard split well method All these cells expressed fine specificity to human recombinant G1 but not to unrelated antigen All the 15 lines expressed a pan T cell marker and 13 of them selectively used the ab T cell receptor Two of ...
Adamalysin-like metalloproteinases with thrombospondin (TS) motifs (ADAMTS)-5 is the multi-domain metalloproteinase that most potently degrades aggrecan proteoglycan in the cartilage and its activity is implicated in the development of osteoarthritis (OA). To generate specific exosite inhibitors for it, we screened a phage display antibody library in the presence of the zinc-chelating active site-directed inhibitor GM6001 (Ilomastat) and isolated four highly selective inhibitory antibodies. Two antibodies were mapped to react with exosites in the catalytic/disintegrin domains (Cat/Dis) of the enzyme, one in the TS domain and one in the spacer domain (Sp). The antibody reacting with the Sp blocked the enzyme action only when aggrecan or the Escherichia coli-expressed aggrecan core protein were substrates, but not against a peptide substrate. The study with this antibody revealed the importance of the Sp for effective aggrecanolytic activity of ADAMTS-5 and that this domain does not interact with sulfated
Wang L, Pawlak E, Johnson PJ, Belknap JK, Alfandari D, Black SJ. Effects of cleavage by a disintegrin and metalloproteinase with thrombospondin motifs-4 on gene expression and protein content of versican and aggrecan in the digital laminae of horses with starch gruel-induced laminitis. Am J Vet Res. 2012 Jul; 73(7):1047-56 ...
swig_c++.tcl # Provides a simple object oriented interface using # SWIGs low level interface. # proc new {objectType handle_r args} { # Creates a new SWIG object of the given type, # returning a handle in the variable "handle_r". # # Also creates a procedure for the object and a trace on # the handle variable that deletes the object when the # handle variable is overwritten or unset upvar $handle_r handle # # Create the new object # eval set handle \[new_$objectType $args\] # # Set up the object procedure # proc $handle {cmd args} "eval ${objectType}_\$cmd $handle \$args" # # And the trace ... # uplevel trace variable $handle_r uw "{deleteObject $objectType $handle}" # # Return the handle so that new can be used as an argument to a procedure # return $handle } proc deleteObject {objectType handle name element op} { # # Check that the object handle has a reasonable form # if {![regexp {_[0-9a-f]*_(.+)_p} $handle]} { error "deleteObject: not a valid object handle: $handle" } # # Remove the ...
I got args halfway into my season and i only got to ride them five times or so, since i do not have a place in the mountains i feel like this may be just to muc
File lib/spec/example/shared_example_group.rb, line 49 def initialize(*args, &example_group_block) set_description(*args) @example_group_block = example_group_block end ...
File lib/spec/example/example_group_factory.rb, line 23 def create_shared_example_group(*args, &example_group_block) # :nodoc: ::Spec::Example::SharedExampleGroup.register(*args, &example_group_block) end ...
11:05:18] ,Darke, So far every file has been named by the primary class inside it, so Args.cc,h has a class Args { definition, etc. The problem is what to do with files that lack class? Ive got the holdover from exult common_types.h, now logically I should rename it to CommonTypes.hpp, just because its the same capitalisation as the other files. But it doesnt contain a CommonTypes class ...
Information on Middlesex University's Research Repository: a online collection of Middlesex University's research outputs
Introduction. Intervertebral disc (IVD) degeneration is a common disorder that affects a significant proportion of the human population. Spontaneously occurring IVD degeneration is also a common problem in dogs, and therefore, the dog is considered an unique animal model for IVD degeneration research. The current purpose of a treatment is ... read more to alleviate pain. Because the current treatments do not solve the problem, there is increasing interest in regenerative therapies, like treatment with TGF-β. In this study, we have investigated whether caveolin-1, acknowledged for its physiological importance in tissue repair and fibrosis, has an additive effect on TGF-β treatment of degenerated canine nucleus pulposus cells (chondrocyte-like nucleus pulposus cells, CLCs). Materials and methods. Degenerated nucleus pulposus cells (CLCs) were used from twelve canine donors; the donors were all chondrodystrophic dogs (Beagles), aged 19 - 32 months, eight females and four males. The CLC pellets ...
We use cookies to enhance your experience on our website. By continuing to use our website, you are agreeing to our use of cookies. You can change your cookie settings at any time.Find out more ...
Aggrecanases, in particular aggrecanase-2 (ADAMTS-5), are considered the principal proteases responsible for aggrecan degradation in osteoarthritis. For this reason, considerable effort has been put on the discovery and development of aggrecanase inhibitors able to slow down or halt the progression of osteoarthritis. We report herein the synthesis and biological evaluation of a series of arylsulfonamido-based hydroxamates as aggrecanase inhibitors. Compound 18 was found to have a nanomolar activity for ADAMTS-5, ADAMTS-4 and MMP-13 and high selectivity over MMP-1 and MMP-14. Furthermore, this compound proved to be effective in blocking ex vivo cartilage degradation without having effect on cell cytotoxicity.
1. *Primorac D., Velleman S., Clark S., Chandrasekaran L., Tanzer M., Rowe, D. Defective aggrecan mRNA processing associated with nanomelia, a heritable chondrodystrophy of chicken. J. Bone Min. Res. 7(1):S132, 1992. 2. *Primorac D., Stover M., Liu S., McKinstry M., Rowe D. Defective Col1A1 mRNA splicing causing a null allele in type I osteogenesis imperfecta. J. Bone Min. Res. 7(1):S132, 1992. 3. *Primorac D., Velleman S., Clark S., Chandrasekaran L., Tanzer M., Rowe D. Defective aggrecan mRNA processing associated with nanomelia, a heritable chondrodystrophy of chicken. 12th Ann. Mtg. East Coast Connective Tissue Society, Philadelphia, March 1993 Hosted by Philadelphia Connective Tissue Group. (Book of abstracts). 4. *Primorac D., Stover M.L., McKinstry BM, Redford-Badwal D., Clark S.H., Rowe D. Reduced type II collagen in nanomelic cultured chondrocytes: An example of extracellular matrix/collagen feedback regulation? 13th Ann. Mtg. East Coast Connective Tissue Society, Baltimore, March 1994 ...
OBJECTIVES: Proteolytic degradation of aggrecan is a hallmark of the pathology of osteoarthritis. The aim of this study was to develop enzyme-linked immunosorbent assay (ELISA) to quantify the serum levels of specific aggrecan fragments generated by
Thank you for your interest in spreading the word about Biochemical Society Transactions.. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.. ...
src/Elasticsearch/Query/QueryBuilder.php public function addFilter(\Elastica\Query\AbstractQuery $filter) public function addPostFilter(\Elastica\Query\AbstractQuery $postFilter) ./src/Elasticsearch/Provider/Visibility/StrategyInterface.php public function elasticAddFilters(User $user, \Elastica\Query\BoolQuery $filter, Visibility $provider); ./clients/base/api/BulkApi.php public function bulkCall(ServiceBase $api, array $args) ./clients/base/api/CalendarEventsApi.php public function updateCalendarEvent(ServiceBase $api, array $args) public function deleteCalendarEvent(ServiceBase $api, array $args) public function updateRecurringCalendarEvent(SugarBean $bean, ServiceBase $api, array &$args) public function deleteRecordAndRecurrences(ServiceBase $api, array $args) public function deleteRecurrences(SugarBean $bean) protected function initializeArgs(array $args, SugarBean $bean = null) protected function adjustStartDate(array &$args) protected function filterOutRecurrenceFields(array $args) ...
El Sistema de Salud de la Universidad de Miami ofrece la atención más avanzada del sur de la Florida. Para más información, llame al 305-243-4000.
Is there a way to have a metaclass similar to our ClasscallMetaclass at all, in Python3?? I just tested, having class Classcall(type): def __call__(cls, *args, **opts): try: classcall = cls.__classcall__ except AttributeError: return type.__call__(cls, *args, **opts) return classcall(cls, *args, **opts) class MyUniqueRepresentation: __metaclass__ = Classcall @staticmethod def __classcall__(cls, *args, **opts): out = super(cls,cls).__new__(cls, *args, **opts) print(classcall got,type(out), cls) cls.__init__(out,*args,**opts) out._reduction = (type(out), args, opts) return out Loading the above into python2, I get ,,, class Foo(MyUniqueRepresentation): ... def __init__(self, a,b): ... self.a = a ... self.b = b ... ,,, A = Foo(A,B) (classcall got, ,class __main__.Foo,, ,class __main__.Foo,) ,,, A._reduction (,class __main__.Foo,, (A, B), {}) But doing the same in python3, it gives ,,, class Foo(MyUniqueRepresentation): ... def __init__(self, a,b): ... self.a = a ... self.b = b ... ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Aggrecan antibody [7D4] (aggrecan) for ELISA, IHC-Fr, IHC-P, IP, WB. Anti-Aggrecan mAb (GTX31216) is tested in Human, Bovine samples. 100% Ab-Assurance.
from genshi.core import Markup from trac.wiki.macros import WikiMacroBase class HelloWorldMacro(WikiMacroBase): """Simple HelloWorld macro. Note that the name of the class is meaningful: - it must end with "Macro" - what comes before "Macro" ends up being the macro name The documentation of the class (i.e. what youre reading) will become the documentation of the macro, as shown by the !MacroList macro (usually used in the WikiMacros page). """ revision = "$Rev$" url = "$URL$" def expand_macro(self, formatter, name, text, args): """Return some output that will be displayed in the Wiki content. `name` is the actual name of the macro (no surprise, here itll be `HelloWorld`), `text` is the text enclosed in parenthesis at the call of the macro. Note that if there are no parenthesis (like in, e.g. [[HelloWorld]]), then `text` is `None`. `args` are the arguments passed when HelloWorld is called using a `#!HelloWorld` code block. """ return Hello World, text = %s, args = %s % \ ...
from genshi.core import Markup from trac.wiki.macros import WikiMacroBase class HelloWorldMacro(WikiMacroBase): """Simple HelloWorld macro. Note that the name of the class is meaningful: - it must end with "Macro" - what comes before "Macro" ends up being the macro name The documentation of the class (i.e. what youre reading) will become the documentation of the macro, as shown by the !MacroList macro (usually used in the WikiMacros page). """ revision = "$Rev$" url = "$URL$" def expand_macro(self, formatter, name, text, args): """Return some output that will be displayed in the Wiki content. `name` is the actual name of the macro (no surprise, here itll be `HelloWorld`), `text` is the text enclosed in parenthesis at the call of the macro. Note that if there are no parenthesis (like in, e.g. [[HelloWorld]]), then `text` is `None`. `args` are the arguments passed when HelloWorld is called using a `#!HelloWorld` code block. """ return Hello World, text = %s, args = %s % \ ...
mikelove: trying out: alias rhelp="Rscript -e args ,- commandArgs(TRUE); help(args[2], package=args[3], help_type=html); Sys.sleep(5) --args" (5 Aug ...
O esperado é algo assim? [09-Nov-2017 04:51:35 UTC] hook http_request_args: 1510203095 [09-Nov-2017 04:51:46 UTC] hook… 2 months ago. ...
Versican is a large extracellular matrix proteoglycan that is present in a variety of human tissues. It is encoded by the VCAN gene. Versican is a large chondroitin sulfate proteoglycan with an apparent molecular mass of more than 1000kDa. In 1989, Zimmermann and Ruoslahti cloned and sequenced the core protein of fibroblast chondroitin sulfate proteoglycan. They designated it versican in recognition of its versatile modular structure. Versican belongs to the lectican protein family, with aggrecan (abundant in cartilage), brevican and neurocan (nervous system proteoglycans) as other members. Versican is also known as chondroitin sulfate proteoglycan core protein 2 or chondroitin sulfate proteoglycan 2 (CSPG2), and PG-M. These proteoglycans share a homologous globular N-terminal, C-terminal, and glycosaminoglycan (GAG) binding regions. The N-terminal (G1) globular domain consists of Ig-like loop and two link modules, and has Hyaluronan (HA) binding properties. Versican occurs in 5 isoforms : V0, ...
CCN2/CTGF, which consists of four domains (IGFBP, VWC, TSP1 and CT domains), regulates the proliferation and differentiation of chondrocytes as well as the production of ECM in cartilage. CCN2/CTGF stimulates aggrecan synthesis in HCS-2/8 chondrocytic cells and primary rabbit chondrocytes [6]. In the present study, for the first time, we provide experimental evidence that CCN2/CTGF binds to aggrecan through its N-terminal domains (IGFBP and VWC domains). Furthermore, we have shown that the N-terminal half of CCN2/CTGF (IGFBP-VWC domain) strongly enhances aggrecan synthesis and secretion by HCS-2/8 chondrocytes. The same domain has been reported to interact with IGF [27], TGF-β and BMP-4 [16]; thus this N-terminal part of CCN2/CTGF may be important in transducing cell-surface signalling into the cells.. Previously, we have shown that the C-terminal CT domain of CCN2/CTGF binds to ECM proteins, such as fibronectin, and enhances cell adhesion to fibronectin [11]. In light of other reports on the ...
We aim at determining the changes in the expression of NF-kB signaling pathway in degenerative intervertebral discs. We collected normal and degenerated intervertebral discs tissues. The normal and degenerated cells were cultivated and their histopathology and immunofluoresence studies were used to observe the position of NF-kB p65 in the cell. We also treated the nucleus pulposus cells with inflammatory factors and inhibitors. Western blot was used to analyze the expression of different proteins. Real time fluorescence-based quantitative PCR was used for observation of NF-kB regulation of change in gene expression. Immunofluorescence showed that in the non-degenerative group the p65 was found in the cytoplasm of the nucleus pulposus cell while in the degenerated cell group the p65 protein was found in the nucleus of the cell. The expression of p65 increased with increase in the degree of degenerative change of the nucleus pulposus cell. RT-PCR showed that the expression of matrix ...
Antarctica is known for its pristine environments. A variety of unsuitable environmental conditions were once thought to render the continent unsuitable for sustaining life. However, metagenomic data have revealed a wealth of species diversity in a range of biotopes.Hypolithons, photosynthetic communities which live under translucent rocks in climatically extreme environments, are an important input source for both carbon (C) and nitrogen (N) in this hyperarid desert environment. Microbial contribution to biogeochemical cycling resulting in fixation of both C and N remains poorly understood. Moreover, there is a reported close interplay between both cycles, with nitrogen being reported to be a limiting factor in carbon assimilation.In this study the diversity of C and N fixing organisms was investigated by using the cbbL and nifH genes as phylogenetic and functional markers. High Molecular weight metagenomic DNA and RNA was extracted from hypolithons. PCR amplification was carried out using cbbL ...
The proteoglycan aggrecan, which is a major structural component of cartilage, has been identified as a candidate autoantigen in rheumatoid arthritis (RA). This is principally due to its degradation early in the disease pathology, its ability to induce an RA-like disease in mouse models and the presence of elevated numbers of reactive T and B cells in RA patients. Studies have also defined an essential requirement for autoantigen-specific B cells as antigen presenting cells (APC) in RA although the cellular mechanisms involved in antigen processing and presentation of joint-derived autoantigens by B cells remains unknown. To investigate the role of autoreactive B cells as APC in RA, I have used two complimentary approaches to generate B cells expressing an aggrecan-specific B cell receptor (BCR). The first was based on a modified monoclonal antibody production protocol and the second involved the transfection of B lymphoma cells with newly generated plasmids encoding an aggrecan-specific BCR. ...
1 1 and polycystin 4 are also expressed in the buy viagra cardiff MM Dudley et al 1998 pronephroi do participate in normal and there is an autosomal dominant medullary cystic disease to describe the expression of desmosomal components during kidney development Glomerulogenesis and nephrogenesis J Clin Endocrinol Metab 18 549a 561 Krapf R Alpern R J Wrong O M Slaney S F a 1993 Developmental regulation and delivery of large irregular sinusoidal capillaries in the. 5 1 1 6 ng ml of 1 mg kga 1 per dose 6a 9 h after the first collagen proteoglycan with heparan sulfate proteoglycan core protein mRNA persisted at relatively high if the disorder Hyp and Gy mice have mutations affecting pronephric function Development 155 2963a 2940 Fu W and Bruns G A John E and F are early tailbud distinct lateral and medial domains exist within native basement membranes black The vascular status is now outdated Impaired oxygen delivery to the orbital septum It can contaminate and grow principally by cell pH of the two ...
Array ( [1516013294] => Array ( [do_pings] => Array ( [40cd750bba9870f18aada2478b24840a] => Array ( [schedule] => [args] => Array ( ) ) ) ) [1516013830] => Array ( [do_pings] => Array ( [40cd750bba9870f18aada2478b24840a] => Array ( [schedule] => [args] => Array ( ) ) ) ) [1516015285] => Array ( [do_pings] => Array ( [40cd750bba9870f18aada2478b24840a] => Array ( [schedule] => [args] => Array ( ) ) ) ) [1516015507] => Array ( [do_pings] => Array ( [40cd750bba9870f18aada2478b24840a] => Array ( [schedule] => [args] => Array ( ) ) ) ) [1516016369] => Array ( [do_pings] => Array ( [40cd750bba9870f18aada2478b24840a] => Array ( [schedule] => [args] => Array ( ) ) ) ) [1516016636] => Array ( [do_pings] => Array ( [40cd750bba9870f18aada2478b24840a] => Array ( [schedule] => [args] => Array ( ) ) ) ) [1516017377] => Array ( [do_pings] => Array ( [40cd750bba9870f18aada2478b24840a] => Array ( [schedule] => [args] => Array ( ) ) ) ) [1516018506] => Array ( [do_pings] => Array ( ...
In the project root set the following alias $: alias render=mvn exec:java -Dexec.mainClass=Main # Using OPSIN to load porphyrin and generate a PDF $: render -Dexec.args="-name porphyrin -pdf" # Highlight one of the pyrrole in porphyrin $: render -Dexec.args="-name porphyrin -pdf -sma n1cccc1" # Show atom numbers $: render -Dexec.args="-name porphyrin -pdf -atom-numbers" # Show CIP labels $: render -Dexec.args="-name (2R)-butan-2-ol -pdf -cip-labels" # Generate a PDF / SVG for ethanol SMILES $: render -Dexec.args="-smi CCO -pdf ethanol.pdf -svg ethanol.svg" # Load a molfile $: render -Dexec.args="-mol ChEBI_6701.mol -pdf chebi-6701.pdf ...
In the project root set the following alias $: alias render=mvn exec:java -Dexec.mainClass=Main # Using OPSIN to load porphyrin and generate a PDF $: render -Dexec.args="-name porphyrin -pdf" # Highlight one of the pyrrole in porphyrin $: render -Dexec.args="-name porphyrin -pdf -sma n1cccc1" # Show atom numbers $: render -Dexec.args="-name porphyrin -pdf -atom-numbers" # Show CIP labels $: render -Dexec.args="-name (2R)-butan-2-ol -pdf -cip-labels" # Generate a PDF / SVG for ethanol SMILES $: render -Dexec.args="-smi CCO -pdf ethanol.pdf -svg ethanol.svg" # Load a molfile $: render -Dexec.args="-mol ChEBI_6701.mol -pdf chebi-6701.pdf ...
Title : seqname Usage : $obj-,seq_id($newval) Function: There are many cases when you make a feature that you do know the sequence name, but do not know its actual sequence. This is an attribute such that you can store the seqname. This attribute should *not* be used in GFF dumping, as that should come from the collection in which the seq feature was found. Returns : value of seqname Args : newvalue (optional ...
Aggrecan (ACAN), also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1, is a protein that in humans is encoded by the ACAN gene. This gene is a member of the lectican (chondroitin sulfate proteoglycan) family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Aggrecan is a proteoglycan, or a protein modified with large carbohydrates; the human form of the protein is 2316 amino acids long and can be expressed in multiple isoforms due to alternative splicing. Aggrecan is a high molecular weight (1x106 < M < 3x106) proteoglycan. It exhibits a bottlebrush structure, in which chondroitin sulfate and keratan sulfate glycosaminoglycan (GAG) chains are attached to an extended protein core. Aggrecan has a molecular mass >2,500 kDa. The core protein (~300 kDa) has around 100 GAG chains attached to it. Aggrecan consists of two globular structural domains (G1 and G2) at ...
Abbaszade I, Liu RQ, Yang F, Rosenfeld SA, Ross OH, Link JR., Ellis DM, Tortorella MD, Pratta MA, Hollis JM, Wynn R, Duke JL, George HJ, Hillman MC, Murphy K, Wiswall BH, Copeland RA, Decicco CP, Bruckner R, Nagase H, Itoh Y, Newton RC, Magolda RL, Trzaskos JM (1999) Cloning and characterization of ADAMTS11, an aggrecanase from the ADAMTS family. J Biol Chem 274: 23443-23450.. Barry FP, Neame PJ, Sasse J, Pearson D (1994) Length variation in the keratan sulfate domain of mammalian aggrecan. Matrix 14: 323-328.. Barry FP, Rosenberg LC, Gaw JU, Koob TJ, Neame PJ (1995) N- and O-linked keratan sulfate on the hyaluronan binding region of aggrecan from mature and immature bovine cartilage. J Biol Chem 270: 20516-20524.. Bredrup C, Knappskog PM, Majewski J, Rodahl E, Boman H (2005) Congenital stromal dystrophy of the cornea caused by a mutation in the decorin gene. Invest Ophthalmol Vis Sci 46: 420-426.. Brown GM, Huckerby TN, Bayliss MT, Nieduszynski IA (1998) Human aggrecan keratan sulfate undergoes ...
Hyaluronan (HA) is widely detected in biological samples and its concentration is most commonly determined by the use of a labeled specific HA binding protein (aggrecan G1-IGD-G2, HABP), employing membrane blotting and sandwich enzyme-linked immunosorbent assay (ELISA)-like methods. However, the detected signal intensity or the quantified value obtained by using these surface-based methods is related to the molecular mass (M) of HA, especially for HA in the low M range below ~150 kDa. At the same mass or mass concentration, higher M HA gives a higher signal than lower M HA. We have experimentally determined the quantitative relationship between the M of HA (in the range 20-150 kDa) and the relative signal intensity in comparison with a standard HA, in a sandwich ELISA-like assay. An M-dependent signal correction factor (SCF) was calculated and used to correct the signal intensity, so that the corrected concentration value would more accurately reflect the true HA concentration in solution. The ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ id Title : id Usage : $obj-,id(3); $id_integer = $obj-,id(); Function: Sets or returns the id of the translation table. IDs are integers from 0 (special ATG-only start) to 25, excluding 7-8 and 17-20 which have been removed. If an invalid ID is given the method returns 1, the standard table. Example : Returns : value of id, a scalar, warn and fall back to 1 (standard table) if specified id is not valid Args : newvalue (optional) name Title : name Usage : $obj-,name() Function: returns the descriptive name of the translation table Example : Returns : A string Args : None tables Title : tables Usage : $obj-,tables() or Bio::Tools::CodonTable-,tables() Function: returns a hash reference where each key is a valid codon table id() number, and each value is the corresponding codon table name() string Example : Returns : A hashref Args : None translate Title : translate Usage : ...
usr/bin/env desres-exec #{ # exec desres-cleanenv -c --user-env \ # -m Python/2.7.1-06A/bin \ # -m destro/0.6.2/lib-python \ # -m scipy/0.12.0-01/lib-python \ # -m destiny/0.4.47-3/lib-python \ # -m molpro/2012.1.12-desres-3-02/serial/bin \ # -m psi4/4.0b5-desres-2-01/bin \ # -- python -u $0 [email protected] #} import sys sys.path.append(/u/en/donchev/pyprogs) sys.path.append(/u/en/donchev/pyprogs/chemsys) sys.path.append(/d/en/ffdevel-0/bin/pyqmdata) sys.path.append(/u/en/donchev/playground/qmdata6/) import math, numpy import subprocess import time import copy import string import units import prepmpr import pth4 as pth import frame import class_qmjob import class_scannmer class MultiScanNmer(object): def __init__(self, finput, opts, molpro_args=[]): self.molpro_args = molpro_args self.opts = opts #self.basopt = self.opts.basopt #self.basene = self.opts.basene #self.basesl = self.opts.basesl self.ncores = self.opts.ncores self.verbose = self.opts.verbose self.rerun = self.opts.rerun self.pinput = ...
1, post_type => kwlogos, post_status => publish, orderby => rand, taxonomy => kwlogos-carousel, term => banner); $banner=get_posts($args); $thumbnail_id = get_post_thumbnail_id($banner[0]->ID); $image = wp_get_attachment_image_src($thumbnail_id, full ...
Anybody ever use this function? It looks like exactly what I need. Im trying to get the content of a couple of resources into fred windows. In classic style, there is no documentation and C-x C-a says the args are named arg-1 arg-2 &key key-1 key-2 key-3 key-4 key-5. Maybe they figure what we dont know cant hurt us? Blake Meike [email protected] ...
A biotinylated complex of aggrecan G1-domain and link protein was used to characterize the distribution of hyaluronan in paraffin-embedded sections of adult human and canine intervertebral disc and cartilage endplate. Limited chondroitinase ABC and trypsin digestions of the sections before staining was utilized to expose hyaluronan potentially masked by aggrecan. Hyaluronan concentration and hyaluronan to uronic acid ratio in different parts of the discs were measured as a background for the histological analysis. Hyaluronan staining was strong in the nucleus pulposus and inner parts of annulus fibrosus of both species, corroborated by biochemical assays of the same compartments. Particularly in human samples, hyaluronan in the interterritorial matrix of nucleus pulposus and annulus fibrosus was readily accessible to the probe without enzyme treatments. In contrast, the cell-associated hyaluronan signal was enhanced after trypsin or limited chondroitinase ABC-treatment of the sections, ...
Chondrodysplasia in the autosomal recessive cartilage matrix deficiency (cmd) mutant is caused by lack of the proteoglycan aggrecan arising from a mutation in the gene. Homozygous cmd/cmd mice are characterized by disorganisation of chondrocytes in the growth plate, disproportionate dwarfism, cleft palate, and perinatal lethality. We have studied the impact of the aggrecan deficiency on the expression of other matrix genes during the differentiation of chondrocytes in the growth plate of cmd/cmd 18.5 day fetuses. Compared with the wild-type, there are significant differences in the growth plates of cmd mutants in the combinations of co-expression of genes encoding the glycoprotein link protein, proteoglycan syndecan 3, collagens α1(X) [Col 1Oa1], α2(XI) [Col 11a2], and the alternative transcripts of α (11) [Col2a1 type IIA form], and α1(IX) [Co1 9 a1 long and short forms]. The discordance of gene expression in cmd chondrocytes may be additional factors contributing to the disrupted cellular ...
Fibulin-2 participates in the assembly of extracellular matrix parts through interactions with multiple ligands and promotes connections between cells and their encircling environment. of Fibulin-2 by ADAMTS-5 can be counteracted by ADAMTS-12, a metalloprotease that interacts with Fibulin-2. General, our outcomes offer immediate proof suggesting that Fibulin-2 can be a book substrate of ADAMTS-5 Abiraterone and that this proteolysis could alter the mobile microenvironment influencing the stability between protumor and antitumor results connected to both Fibulin-2 and the ADAMTSs metalloproteases. biochemical techniques [19]. By comparison, Fibulin-1 is highly resistant to the proteolysis mediated by this gelatinase [18] also. ADAMTS-5 and ADAMTS-4 are members of the ADAMTS family members of secreted metalloproteases Abiraterone [20]. Both digestive enzymes are also known as aggrecanases still to pay their capability to cleave cartilage aggrecan [21]. Additionally, aggrecanases can degrade ECM ...
Although the etiology of intervertebral disc degeneration is poorly understood, one possible approach to regulate the process of intervertebral disc degeneration may include the inhibition of apoptosis. We investigated the anti-apoptotic effects of b
The nucleus pulposus is the jelly-like substance in the middle of the disc. It functions to distribute loading-pressure in all directions within each disc. The nucleus pulposus consists of chondrocyte-like cells, collagen fibrils, and proteoglycan aggrecans, which allow it to draw water into the nucleus pulposus.As we age, the intervertebral disc begins to degenerate and dehydrate after repetitive exposure to ordinary wear and tear or mechanical loading. The intervertebral disc in older spines has a smaller concentration of proteoglycans in the nucleus pulposus, causing a decreased ability to stay hydrated and an altered disc structure, often in the appearance of a thinner disc. The inner annulus fibrosous then desiccates, causing the outer annulus fibrosous to deteriorate, leading to a loss of overall structure of the functional spinal unit. Over time, degeneration can lead to disc bulges, protrusions and extrusions.[1]Trauma on the Degenerative SpineTrauma, no matter how mild, has the ...
Ms. Bhumika D. Patel is working as Assistant professor in Department of Pharmaceutical Chemistry. She has 6 year and 11 months of teaching experience. She has attended many national and international level seminars and conferences. She has 7 international publications and 12 presentations in her credit. Currently she is pursuing Ph. D under Prof. Manjunath Ghate, Director and Head, Dept. of Pharmaceutical Chemistry, Institute of Pharmacy, Nirma Univeristy. She has received a 3 year minor research project grant of Rs. 5,00,000/- from GUJCOST in the year 2013. She has guided 4 PG students as co-guide and her area of research includes design and synthesis of different heterocycles under various therapeutic classes. She has a sound knowledge of drug designing softwares like Sybyl, Discovery Studio, Gold, Schrodinger etc. She is a life member of professional bodies like APTI, IPA and ISCB(Indian Society of Chemists and Biologists ...
Kaleem, Bilal, "Low-Energy Impact of Human Cartilage: Predictors for Fracturing the Network of Collagen" (2016). Masters Theses. 948 ...
Kuzhuvelil B. Harikumar, Ajaikumar B. Kunnumakkara, Nobuo Ochi, Zhimin Tong, Amit Deorukhkar, Bokyung Sung, Lloyd Kelland, Stephen Jamieson, Rachel Sutherland, Tony Raynham, Mark Charles, Azadeh Bagherzadeh, Caroline Foxton, Alexandra Boakes, Muddasar Farooq, Dipen Maru, Parmeswaran Diagaradjane, Yoichi Matsuo, James Sinnett-Smith, Juri Gelovani, Sunil Krishnan, Bharat B. Aggarwal, Enrique Rozengurt, Christopher R. Ireson and Sushovan Guha ...
I was logged in when the first server went live and I had (still have, written down) a key to help open it, but got no message about a reward once I entered the key (and I realized after that that my MSN profile was empty of my address and personal info ...
Use Bio-Rads PrimePCR assays, controls, templates for your target gene. Every primer pair is optimized, experimentally validated, and performance guaranteed.
class BubbleSort { public static void sort(int[] a) { int i; int j; for (i = 0 ; i , a.length; i++) { /* move the largest number up */ for (j = 0 ; j , a.length - i - 1; j++) { if (a[j] , a[j+1]) ArrayUtil.swap(a, j, j + 1); } } } } public class BubbleSortTest { public static void main(String[] args) { int[] a = ArrayUtil.randomIntArray(20, 100); ArrayUtil.print(a); BubbleSort.sort(a); ArrayUtil.print(a ...
The nonlinear, rate-independent behavior of human intervertebral discs is studied with a finite element model which incorporates a nonlinear elastic constitutive relation for the annulus fibrosis. The elastic coefficients and a nonlinear constitutive parameter for the annulus fibrosis were obtained by matching experimental results for the discs load-deflection behavior. The axisymmetric finite el
OBJECTIVE: To determine the effects of hypoxia on both anabolic and catabolic pathways of metabolism in human articular cartilage and to elucidate the roles played by hypoxia-inducible factors (HIFs) in these responses. METHODS: Normal human articular cartilage from a range of donors was obtained at the time of above-the-knee amputations due to sarcomas not involving the joint space. Fresh cartilage tissue explants and isolated cells were subjected to hypoxia and treatment with interleukin-1α. Cell transfections were performed on isolated human chondrocytes. RESULTS: Using chromatin immunoprecipitation, we found that hypoxia induced cartilage production in human tissue explants through direct binding of HIF-2α to a specific site in the master-regulator gene SOX9. Importantly, hypoxia also suppressed spontaneous and induced destruction of human cartilage in explant culture. We found that anticatabolic responses were predominantly mediated by HIF-1α. Manipulation of the hypoxia-sensing pathway through
An In Vitro Culture System to Study Human Mesenchymal Stem Cell / Nucleus Pulposus Cell Interactions : Implications for Intervertebral Disc Regeneration ...
Despite the high prevalence of intervertebral disc disease, little is known about changes in intervertebral disc cells and their regenerative potential with ageing and intervertebral disc degeneration. Here we identify populations of progenitor cells that are Tie2 positive (Tie2+) and disialoganglioside 2 positive (GD2+), in the nucleus pulposus from mice and humans. These cells form spheroid colonies that express type II collagen and aggrecan. They are clonally multipotent and differentiated into mesenchymal lineages and induced reorganization of nucleus pulposus tissue when transplanted into non-obese diabetic/severe combined immunodeficient mice. The frequency of Tie2+ cells in tissues from patients decreases markedly with age and degeneration of the intervertebral disc, suggesting exhaustion of their capacity for regeneration. However, progenitor cells (Tie2+GD2+) can be induced from their precursor cells (Tie2+GD2-) under simple culture conditions. Moreover, angiopoietin-1, a ligand of Tie2, is
J:83512 Hikake T, Mori T, Iseki K, Hagino S, Zhang Y, Takagi H, Yokoya S, Wanaka A, Comparison of expression patterns between CREB family transcription factor OASIS and proteoglycan core protein genes during murine tooth development. Anat Embryol (Berl). 2003 Apr;206(5):373-80 ...
Most human cancers acquire tens to hundreds of somatic mutations (termed the "tumor mutome") during their development (1). Each of these mutations has the potential to generate one or more novel T-cell antigens (termed "neoepitopes") uniquely specific to each individual patients tumor. Because these neoepitopes are not present in the germline, and are not encountered until after the onset of oncogenesis, repertoires of high-avidity T cells capable of recognizing them may avoid central tolerance and escape deletion in the thymus. For these reasons, numerous investigators have proposed that the tumor mutome provides an attractive source of antigenic targets for developing patient-specific tumor vaccines (2-5).. Because it is already possible to rapidly and comprehensively identify tumor mutations using next-generation DNA- and RNA-sequencing technologies (1), the first technical hurdle for the development of this approach has been overcome. However, it may not be practical to target the full ...
Blood Pressure - Corolater (Brand name: cardizem) Diltiazem, Acalix,Acasmul,Adizem,Alandiem,Aldizem,Altiazem,Altizem,Angiazem,Angiodrox,Angiolong,Angiotrofin,Angiozem,Angitil,Angizem,Balcor,Beatizem,Bi-tildiem,Blocalcin,Cal-antagon,Calnurs,Cardiser,Cardium,Carreldon,Cartia,Channel,Clarute,Clobendian,Cohlen,Conductil,Coramil,Coras,Corazem,Cordisil,Cordizem,Coridil,Corodrox,Coroherser,Corolater,Cortiazem,Corzem,Cronodine,Daltazen gmp,Dasav,Dazil,Deltazen lp,Denazox,Diacor,Diacordin,Dial,Diazem,Dil-sanorania,Dilaclan,Dilacor xr,Diladel,Dilatam,Dilcardia,Dilcontin,Dilcor,Dilem,Dilfar,Dilgard,Dilgina,Diliter,Dilmacor,Dilmen,Dilocard,Dilrene,Dilsal,Dilt-cd,Dilta-hexal,Diltahexal,Diltam,Diltaretard,Diltelan,Diltenk,Dilti,Diltiagamma,Diltiangina,Diltiastad,Diltiasyn,Diltiax,Diltia xt,Diltiazemum,Diltiem,Dilti sr,Diltiwas,Diltor,Diltzac,Dilzacard,Dilzem,Dilzen-g,Dilzene,Dinisor,Dipen,Doclis,Dodexen,Elvesil,Entrydil,Ergoclavin,Ergolan,Etizem,Etyzem,Evascon,Frotty,Grifodilzem,Hart,Hemarekeat,Herbesser,Hesor
Dr. Kenil A. Lathia - Fellowship in aesthetic medicine, Dr. Fatema Faizullabhoy - MDS, Dr. Dipen Patel - BDS, Dr. Jugal Sherdiwala - MDS, Dr. Ravi Agarwal, Dr. Manish Sinha - MDS, Dr. Prashant Rupabhinda - BDS, Dr. Rateesha Bawa - MDS, Dr. YOGESH KANTIBHAI TAVETHIYA - BDS, Dr. dhaval patel - BDS, Dr. Tejas Gandhi - BDS, Dr. Bhavesh D. Trivedi - MDS, Dr. Sejal Shah, Dr. Jaydeep Mali - MDS
Warning: Declaration of PLL_Walker_List::walk($elements, $args = Array) should be compatible with Walker::walk($elements, $max_depth, ...$args) in /home/hydrog63/public_html/wp-content/plugins/polylang/include/walker-list.php on line 0 ...
During appendicular skeletal development, the bi-potential cartilage anlagen gives rise to transient cartilage, which is eventually replaced by bone, and to articular cartilage that caps the ends of individual skeletal elements. While the molecular mechanism that regulates transient cartilage differentiation is relatively well understood, the mechanism of articular cartilage differentiation has only begun to be unraveled. Furthermore, the molecules that coordinate the articular and transient cartilage differentiation processes are poorly understood. Here, we have characterized in chick the regulatory roles of two transcription factors, NFIA and GATA3, in articular cartilage differentiation, maintenance and the coordinated differentiation of articular and transient cartilage. Both NFIA and GATA3 block hypertrophic differentiation. Our results suggest that NFIA is not sufficient but necessary for articular cartilage differentiation. Ectopic activation of GATA3 promotes articular cartilage ...
Background: Recent studies have provided evidence that integrins play roles in recognition of mechanical stimuli and its translation into a cellular response. Integrin signaling may be regulated by a number of mechanisms including accessory proteins such as CD98 (4F2 antigen). Objectives: To determine CD98 expression by human articular chondrocytes and its involvement in human articular mechanotransduction. Methods: CD98 expression was assessed by immunostaining of cryostat sections of snap frozen articular cartilage and in cultured cells by western blotting. Chondrocytes enzymatically isolated from macroscopically normal and osteoarthritic (OA) articular cartilage were grown in short term, primary monolayer culture and used in a resting state or following mechanical stimulation at 0.33Hz. Results: Human articular chondrocytes express CD98 and immunoreactivity revealed a similar heterogeneous pattern of CD98 in both normal and osteoarthritic (OA) human articular cartilage. No role of CD98 was detected
Prudnikova, K., E. J. Vresilovic and M. Marcolongo (2012). "New Strategy for Treatment of Lower Back Pain: Molecular Restoration of Intervertebral Disc Using an Injection of Biomimetic Aggrecan." The Spine Journal 12(9): S30.. Massey, C. J., C. C. van Donkelaar, E. Vresilovic, A. Zavaliangos and M. Marcolongo (2012). "Effects of aging and degeneration on the human intervertebral disc during the diurnal cycle: A finite element study." Journal of Orthopaedic Research 30(1): 122-128.. Kirzner, Y., M. Marcolongo and S. K. Bhatia (2012). "Advances in Biomaterials for the Treatment of Intervertebral Disc Degeneration." Journal of Long-Term Effects of Medical Implants 22(1): 73-84.. Jost, M., K. Barbee, D. Rothstein, N. Robertson, K. Berkowitz and M. Marcolongo (2012). "Modeling L-Selectin Mediated Attachment Strength during Embryo Implantation." Gender Medicine: The Journal for the Study of Sex & Gender Differences 9(1): S95-S96.. Green, A. P., E. Chen, K. Pourrezaei and M. Marcolongo (2011). ...
The composition of the extracellular matrix of cartilage dictates its mechanical properties. Proteoglycans (PG) and collagen are two important structural components of the cartilage extracellular matrix. The ability to measure changes both in the amount and distribution of cartilage matrix constituents is essential in understanding early pathological changes of joint diseases. Previous studies hav
Degenerative disc disease (DDD) describes the natural breakdown of anintervertebral disc of the spine. Despite its name, DDD is not considered a disease, nor is it progressively degenerative. On the contrary, disc degeneration is often the effect of natural daily stresses and minor injuries that cau
PHILLIPS, Kate Louise Eve, JORDAN-MAHY, Nikki, NICKLIN, Martin J. H. and LE MAITRE, Christine Lyn (2013). Interleukin-1 receptor antagonist deficient mice provide insights into pathogenesis of human intervertebral disc degeneration. Annals of the Rheumatic Diseases, 72, 1860-1867 ...
Background Osteoarthrosis is characterized by cartilage erosion, proteolysis of aggrecan and collagen, and disturbed rates of synthesis of aggrecan and hyaluronan by chondrocytes, with hyaluronan...
Physical work, not the main cause of intervertebral disc degeneration Even if you do not suffer from pain in the intervertebral discs in your lower back, it is a sure bet that you know someone who does. Yet, surprisingly little is known about what causes this disc degeneration . ...
Principal Investigator:ITO Akira, Project Period (FY):1997 - 1998, Research Category:Grant-in-Aid for Scientific Research (C), Section:一般, Research Field:老化(加齢)
Callstack: at Proteins/JCSG/282372 at Template:Protein at Template:Pathway at tmdb.RecordList MindTouch.Deki.Script.Runtime.DekiScriptRemoteException: Access denied for user [email protected] (using password: YES) at MindTouch.Deki.Script.Runtime.TargetInvocation.DekiScriptRemoteInvocationTarget.InvokeList (MindTouch.Deki.Script.DekiScriptRuntime runtime, MindTouch.Deki.Script.Expr.DekiScriptList args) [0x00000] in ,filename unknown,:0 at MindTouch.Deki.Script.Runtime.TargetInvocation.ADekiScriptInvocationTarget.Invoke (MindTouch.Deki.Script.DekiScriptRuntime runtime, MindTouch.Deki.Script.Expr.DekiScriptLiteral args) [0x00000] in ,filename unknown,:0 at MindTouch.Deki.Script.DekiScriptRuntime.Invoke (Location location, MindTouch.Dream.XUri uri, MindTouch.Deki.Script.Expr.DekiScriptLiteral args, MindTouch.Deki.Script.Runtime.DekiScriptEnv env) [0x00000] in ,filename unknown,:0 ...
Callstack: at Proteins/JCSG/2raa at Template:Protein at Template:Pathway at tmdb.RecordList MindTouch.Deki.Script.Runtime.DekiScriptRemoteException: Access denied for user [email protected] (using password: YES) at MindTouch.Deki.Script.Runtime.TargetInvocation.DekiScriptRemoteInvocationTarget.InvokeList (MindTouch.Deki.Script.DekiScriptRuntime runtime, MindTouch.Deki.Script.Expr.DekiScriptList args) [0x00000] in ,filename unknown,:0 at MindTouch.Deki.Script.Runtime.TargetInvocation.ADekiScriptInvocationTarget.Invoke (MindTouch.Deki.Script.DekiScriptRuntime runtime, MindTouch.Deki.Script.Expr.DekiScriptLiteral args) [0x00000] in ,filename unknown,:0 at MindTouch.Deki.Script.DekiScriptRuntime.Invoke (Location location, MindTouch.Dream.XUri uri, MindTouch.Deki.Script.Expr.DekiScriptLiteral args, MindTouch.Deki.Script.Runtime.DekiScriptEnv env) [0x00000] in ,filename unknown,:0 ...
BrianObjectExceptionTraceback (most recent call last) ,ipython-input-11-d086eea0b2de, in ,module,() 3 4 G = NeuronGroup(1, eqs) ----, 5 run(100*ms) /home/marcel/programming/brian2/brian2/units/fundamentalunits.pyc in new_f(*args, **kwds) 2353 get_dimensions(newkeyset[k])) 2354 -, 2355 result = f(*args, **kwds) 2356 if result in au: 2357 if au[result] == bool: /home/marcel/programming/brian2/brian2/core/magic.pyc in run(duration, report, report_period, namespace, profile, level) 369 370 return magic_network.run(duration, report=report, report_period=report_period, --, 371 namespace=namespace, profile=profile, level=2+level) 372 run.__module__ = __name__ 373 /home/marcel/programming/brian2/brian2/core/magic.pyc in run(self, duration, report, report_period, namespace, profile, level) 229 self._update_magic_objects(level=level+1) 230 Network.run(self, duration, report=report, report_period=report_period, --, 231 namespace=namespace, profile=profile, level=level+1) 232 233 def store(self, ...
BrianObjectExceptionTraceback (most recent call last) ,ipython-input-11-d086eea0b2de, in ,module,() 3 4 G = NeuronGroup(1, eqs) ----, 5 run(100*ms) /home/marcel/programming/brian2/brian2/units/fundamentalunits.pyc in new_f(*args, **kwds) 2353 get_dimensions(newkeyset[k])) 2354 -, 2355 result = f(*args, **kwds) 2356 if result in au: 2357 if au[result] == bool: /home/marcel/programming/brian2/brian2/core/magic.pyc in run(duration, report, report_period, namespace, profile, level) 369 370 return magic_network.run(duration, report=report, report_period=report_period, --, 371 namespace=namespace, profile=profile, level=2+level) 372 run.__module__ = __name__ 373 /home/marcel/programming/brian2/brian2/core/magic.pyc in run(self, duration, report, report_period, namespace, profile, level) 229 self._update_magic_objects(level=level+1) 230 Network.run(self, duration, report=report, report_period=report_period, --, 231 namespace=namespace, profile=profile, level=level+1) 232 233 def store(self, ...
url(r^password_change/done/$, lambda *args, **kwargs: self.password_change_done(*args, **kwargs), name=%sadmin_password_change_done % self.name), ...
A free platform for explaining your research in plain language, and managing how you communicate around it - so you can understand how best to increase its impact.
sys_var (sys_var_chain *chain, const char *name_arg, const char *comment, int flag_args, ptrdiff_t off, int getopt_id, enum get_opt_arg_type getopt_arg_type, SHOW_TYPE show_val_type_arg, longlong def_val, PolyLock *lock, enum binlog_status_enum binlog_status_arg, on_check_function on_check_func, on_update_function on_update_func, const char *substitute, int parse_flag ...
You need to allow cookies if you want the configuration to be stored. If not, remember/bookmark the right URL that will be provided after submitting changes. You can override the settings by providing an extra arg to the URL (e.g. &buildd=1 if you want to display the buildd column). These args will not change the custom settings you defined before (the cookie is not modified ...
引用Abcams Anti-CD68抗体[PG-M1] (ab783)的参考文献列表。为您列举引用本产品的发表文章,并提供信息包括论文文献数据库中的检索编号以便您搜寻文章
PHILLIPS, K.L.E., CULLEN, K., CHIVERTON, N., MICHAEL, A.L.R., COLE, A.A., BREAKWELL, L.M., HADDOCK, G., BUNNING, R.A.D, CROSS, A.K. and LE MAITRE, C.L. (2015). Potential roles of cytokines and chemokines in human intervertebral disc degeneration: interleukin-1 is a master regulator of catabolic processes. Osteoarthritis and Cartilage, 23 (7), 1165-1177 ...
Sciences, University of Southern California, Los Angeles, CA, U.S.A. "Medicine provides the means to treat diseases. Knowledge is the foundation of good health." E. J. Lien. Osteoarthritis (OA) is a chronic joint disease that is estimated to affect almost 5 million Canadians (16% of the population) by the year 2016.1 This degenerative disorder is one of the primary causes of pain and long-term disability in the elderly. The disease is characterized by the progressive deterioration of the articular cartilage, the protective "cushion" at the articulating surfaces of bones. This degenerative process is caused primarily by a defect in the metabolism of the component macromolecules including proteoglycans (aggrecans) and type II collagen.. The non-steroidal anti-inflammatory drugs (NSAIDs) like aspirin, ibuprofen, indomethacin and piroxicam are the most widely used medications for the treatment of patients with symptomatic OA.. ...
European Cells & Materials Journal - Open and Free Access - The Official Research Journal of AOCMF, AOTrauma, European Orthopaedic Research Society (EORS), Swiss Society for Biomaterials (SSB) and Tissue & Cell Engineering Society (TCES)
Chemically modified glucosamine inhibits the release of proteoglycan in a model system of articular cartilage degradation [Abstract]. International Journal of Experimental Pathology 90 (1) , A78-A79. 10.1111/j.1365-2613.2008.00612.x ...
TY - JOUR. T1 - The characterization of versican and its message in human articular cartilage and intervertebral disc. AU - Sztrolovics, Robert. AU - Grover, Judy. AU - Cs-Szabo, Gabriella. AU - Shi, Shui Liang. AU - Zhang, Yiping. AU - Mort, John S.. AU - Roughley, Peter J.. PY - 2002. Y1 - 2002. N2 - Splicing variation of the versican message and size heterogeneity of the versican core protein were analyzed in human articular cartilage and intervertebral disc. Splicing variation of the message was studied by PCR analysis to detect the presence or absence of exons 7 and 8, which encode large chondroitin sulfate attachment regions. At all ages in normal cartilage from the third trimester fetus to the mature adult, the presence of the versican isoform possessing exon 8 but not exon 7 (V1) could be readily detected. The message isoforms possessing neither exon 7 nor 8 (V3) or both exons 7 and 8 (V0) were only detectable in the fetus, and the isoform possessing only exon 7 (V2) was never detected. ...

Aggrecans | Profiles RNSAggrecans | Profiles RNS

"Aggrecans" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject ... This graph shows the total number of publications written about "Aggrecans" by people in this website by year, and whether " ... Below are the most recent publications written about "Aggrecans" by people in Profiles. ...
more infohttps://profiles.umassmed.edu/display/121946

Aggrecans | Colorado PROFILESAggrecans | Colorado PROFILES

"Aggrecans" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject ... This graph shows the total number of publications written about "Aggrecans" by people in this website by year, and whether " ... Below are the most recent publications written about "Aggrecans" by people in Profiles. ...
more infohttps://profiles.ucdenver.edu/display/207887

Brevican
     Summary Report | CureHunterBrevican Summary Report | CureHunter

Aggrecans (Aggrecan) 3. Messenger RNA (mRNA) 4. Complementary DNA (cDNA) 5. Versicans (Versican) ...
more infohttp://www.curehunter.com/public/keywordSummaryD058581.do

Triflex - GNC Joint Product - ProgressiveHealth.comTriflex - GNC Joint Product - ProgressiveHealth.com

... chondroitin sulfate refers to a group of glycosaminoglycans called aggrecans. Aggrecans aggregate to form the cartilage. ... Aggrecans such as chondroitin bind to hyaluronic acid to form superstructures of negatively charged aggregates. These ...
more infohttp://www.progressivehealth.com/triflex.htm

US20010014475A1 - Method for fabricating cell-containing implants 
      - Google PatentsUS20010014475A1 - Method for fabricating cell-containing implants - Google Patents

102000016284 Aggrecans Human genes 0 description 2 * 210000001188 Cartilage, Articular Anatomy 0 description 2 ...
more infohttps://patents.google.com/patent/US20010014475A1/en

Miller, R. J.<...Miller, R. J.<...

Our laboratory is interested in the role of receptors in the regulation of nerve cell function.We are particularly interested in the role of cytokines in nerve cell biology.The effects of cytokines on neural stem cell development and in the genesis of chronic pain syndromes are of current interest.We study these phenomena using biochemistry,molecular biology,electrophysiology,mouse genetics and imaging.Projects in our laboratory involve the development of drugs that act on cytokine receptors as well as the molecular basis for pain in osteoarthritis and diabetic neuropathy. ...
more infohttps://www.scholars.northwestern.edu/en/persons/richard-j-miller

Identification of two novel mutations in the COMP gene in six families with pseudoachondroplasiaIdentification of two novel mutations in the COMP gene in six families with pseudoachondroplasia

... aggrecans and collagens. COMP is a pentameric glycoprotein of the cartilage extracellular matrix that is localized ...
more infohttps://www.spandidos-publications.com/10.3892/mmr.2016.5486

Gene expression profiling of mouse articular and growth plate cartilage<...Gene expression profiling of mouse articular and growth plate cartilage<...

TY - JOUR. T1 - Gene expression profiling of mouse articular and growth plate cartilage. AU - Yamane, Shintaro. AU - Cheng, Ewana. AU - You, Zongbing. AU - Reddi, A Hari. PY - 2007/9. Y1 - 2007/9. N2 - Articular cartilage is recalcitrant to repair and regeneration. Tissue engineering and regenerative medicine are potential strategies to treat the damage to articular cartilage. A thorough understanding of the gene expression profiles in articular cartilage and growth plate chondrocytes will be an important prerequisite for tissue engineering of cartilage. Regeneration is a recapitulation of embryonic development and morphogenesis. We used laser capture microdissection to capture the surface articular chondrocytes and the resting zone chondrocytes of growth plate from 14-day-old C57BL/6J mice. Total RNA was individually purified, pooled, and amplified by two rounds of in vitro transcription. Labeled cRNA probes were analyzed using the Affymetrix GeneChip® Mouse Genome 430 2.0 Array. We identified ...
more infohttps://ucdavis.pure.elsevier.com/en/publications/gene-expression-profiling-of-mouse-articular-and-growth-plate-car

Mezőgazdasági Biotechnológiai Kutatóintézet - Research Output
     - Hungarian ConsortiumMezőgazdasági Biotechnológiai Kutatóintézet - Research Output - Hungarian Consortium

Aigner, B., Pambalk, K., Reichart, U., Besenfelder, U., Bösze, Z., Renner, M., Günzburg, W. H., Wolf, E., Müller, M. & Brem, G., ápr. 21 1999, In : Biochemical and Biophysical Research Communications. 257, 3, p. 843-850 8 p.. Research output: Article ...
more infohttps://hungary.pure.elsevier.com/hu/organisations/agricultural-biotechnology-center/publications/?page=6

Aggrecan - WikipediaAggrecan - Wikipedia

Proteases capable of degrading aggrecans are called aggrecanases, and they are members of the ADAM (A Disintegrin And ... Nap RJ, Szleifer I (November 2008). "Structure and interactions of aggrecans: statistical thermodynamic approach". Biophys. J. ...
more infohttps://en.wikipedia.org/wiki/Aggrecan

Prospective evaluation of serum biomarker levels and cartilage repair by autologous chondrocyte transplantation and subchondral...Prospective evaluation of serum biomarker levels and cartilage repair by autologous chondrocyte transplantation and subchondral...

HA plays the key role in immobilizing aggrecans in articular cartilage; this balances the tension and compressive resilience in ... HA decreases the molecular size of the cartilage matrix and increases its proportion to the aggrecans by age-related change [25 ...
more infohttps://arthritis-research.biomedcentral.com/articles/10.1186/ar2709

Full text] New developments in the treatment of osteoarthritis - focus on b | OARRRFull text] New developments in the treatment of osteoarthritis - focus on b | OARRR

Recent studies support the promise of using lubricin as a therapy for OA.6 Aggrecans are formed by a core protein which is ... Other proteins include noncollagenous, aggrecans, leucine-rich repeated, structural, regulatory, and others (Table 1, Figures 1 ... MMP-13 being the most involved in cleaving type II collagen and aggrecans and MMP-7 as an early predictor of OA, as much as 10 ... 47 It has been proven that IL-10 is involved in stimulating the synthesis of type II collagen and aggrecans and decreases TNFα ...
more infohttps://www.dovepress.com/new-developments-in-the-treatment-of-osteoarthritis-ndash-focus-on-bio-peer-reviewed-fulltext-article-OARRR

Synthesis and sorting of proteoglycans | Journal of Cell ScienceSynthesis and sorting of proteoglycans | Journal of Cell Science

1996). Variations in the chondroitin sulfate-protein linkage region of aggrecans from bovine nasal and human articular ...
more infohttps://jcs.biologists.org/content/113/2/193?ijkey=3a3e202747517f8246880c79793767efe0c87d30&keytype2=tf_ipsecsha

Direct linkDirect link

In cartilage, ECM forms a major fraction of the tissue, type II collagen and aggrecans being the most abundant macromolecules. ...
more infohttp://umu.diva-portal.org/smash/record.jsf?pid=diva2%3A843208

Barry Katz - Publications
     - Indiana University School of MedicineBarry Katz - Publications - Indiana University School of Medicine

Rethman, M. P., Beitrán-Aguilar, E. D., Billings, R. J., Burne, R. A., Clark, M., Donly, K. J., Hujoei, P. P., Katz, B., Milgrom, P., Sohn, W., Stamm, J. W., Gene Watson, W., Wolff, M., Wright, J. T., Zero, D., Krishna Aravamudhan, A., Frantsve-Hawley, J. & Meyer, D. M., Sep 2011, In : Journal of the American Dental Association. 142, 9, p. 1065-1071 7 p.. Research output: Contribution to journal › Article ...
more infohttps://indiana.pure.elsevier.com/en/persons/barry-katz/publications/?type=%2Fdk%2Fatira%2Fpure%2Fresearchoutput%2Fresearchoutputtypes%2Fcontributiontojournal%2Farticle&page=1

Satoshi Hirohata - Research Output
     - Okayama UniversitySatoshi Hirohata - Research Output - Okayama University

Hirohata, A., Yamamoto, K., Miyoshi, T., Hatanaka, K., Hirohata, S., Yamawaki, H., Komatsubara, I., Murakami, M., Hirose, E., Sato, S., Ohkawa, K., Ishizawa, M., Yamaji, H., Kawamura, H., Kusachi, S., Murakami, T., Hina, K. & Ohe, T., Mar 9 2010, In : Journal of the American College of Cardiology. 55, 10, p. 976-982 7 p.. Research output: Contribution to journal › Article ...
more infohttps://okayama.pure.elsevier.com/en/persons/satoshi-hirohata/publications/

CARTILAGE - howMedCARTILAGE - howMed

Aggrecans. n large proteoglycan molecules. Protein core. Glycosaminoglycans (chondroitin 4-sulfate and chondroitin 6-sulfate) ...
more infohttp://howmed.net/anatomy/histology/cartilage-2/

Extracellular Matrix Proteins  < Scleroproteins  << Proteins (Protein Science)  <<< Compounds of Life  @...Extracellular Matrix Proteins < Scleroproteins << Proteins (Protein Science) <<< Compounds of Life @...

Aggrecans In our body, an Aggrecans, as an extracellular matrix protein (ecm protein), is a large hyaluronan-containing ... Aggrecans *Cartilage Oligomeric Matrix Protein *CCN Intercellular Signaling Proteins *Connective Tissue Growth Factor *Cysteine ...
more infohttp://wellnessadvocate.com/?dgl=70212

Characterization of dermacan, a novel zebrafish lectican gene, expressed in dermal bones<...Characterization of dermacan, a novel zebrafish lectican gene, expressed in dermal bones<...

TY - JOUR. T1 - Characterization of dermacan, a novel zebrafish lectican gene, expressed in dermal bones. AU - Kang, Jeong Suk. AU - Oohashi, Toshitaka. AU - Kawakami, Yasuhiko. AU - Bekku, Yoko. AU - Izpisúa Belmonte, Juan Carlos. AU - Ninomiya, Yoshifumi. PY - 2004/3. Y1 - 2004/3. N2 - We report here the isolation and characterization of a cDNA encoding zebrafish dermacan, a novel member of hyaluronan (HA)-binding proteoglycans, which was termed after its characteristic expression in the zebrafish dermal bones. The deduced protein sequence shares the typical modular elements of lecticans. Sequence comparison covering the C-terminal globular domain demonstrated that dermacan shows high homology with zebrafish versican but is distinct from any other identified lecticans. Genomic DNA analysis demonstrated that dermacan and versican were encoded by distinct genes in the zebrafish genome. The expression of dermacan is initiated in the sclerotome and cephalic paraxial mesoderm at 16 h ...
more infohttps://okayama.pure.elsevier.com/en/publications/characterization-of-dermacan-a-novel-zebrafish-lectican-gene-expr

Comparison of transforming growth factor-beta1 and lovastatin on differentiating mesenchymal stem cells toward nucleus pulposus...Comparison of transforming growth factor-beta1 and lovastatin on differentiating mesenchymal stem cells toward nucleus pulposus...

TY - JOUR. T1 - Comparison of transforming growth factor-beta1 and lovastatin on differentiating mesenchymal stem cells toward nucleus pulposus-like phenotype. T2 - An in vitro cell culture study. AU - Yang, Shu Hua. AU - Yang, Kai Chiang. AU - Chen, Chih Wei. AU - Huang, Ting Chun. AU - Sun, Yuan Hui. AU - Hu, Ming Hsiao. PY - 2019/1/1. Y1 - 2019/1/1. N2 - Study Design: In vitro cell culture study. Purpose: This study aims to investigate the impact of transforming growth factor-beta1 (TGF-ß1) and lovastatin on differentiating human mesenchymal stem cells (MSCs) toward nucleus pulposus (NP)-like phenotype. Overview of Literature: MSCs offer a cell source to the cell-based therapy for intervertebral disc degeneration. TGF-ß1 is used to induce MSCs to differentiate into NP-like cells; however, an undesired expression of collagen type I has been reported. Statins reportedly stimulate expression of bone morphogenetic protein-2 (BMP-2) and promote the chondrogenic phenotype to NP cells. However, ...
more infohttps://tmu.pure.elsevier.com/zh/publications/comparison-of-transforming-growth-factor-beta1-and-lovastatin-on-

Fibrin and poly(lactic-co-glycolic acid) hybrid scaffold promotes early chondrogenesis of articular chondrocytes: An in vitro...Fibrin and poly(lactic-co-glycolic acid) hybrid scaffold promotes early chondrogenesis of articular chondrocytes: An in vitro...

TY - JOUR. T1 - Fibrin and poly(lactic-co-glycolic acid) hybrid scaffold promotes early chondrogenesis of articular chondrocytes. T2 - An in vitro study. AU - ShaBan, Munirah. AU - Kim, Soon Hee. AU - Idrus, Ruszymah. AU - Khang, Gilson. PY - 2008. Y1 - 2008. N2 - Background. Synthetic- and naturally derived- biodegradable polymers have been widely used to construct scaffolds for cartilage tissue engineering. Poly(lactic-co-glycolic acid) (PLGA) are bioresorbable and biocompatible, rendering them as a promising tool for clinical application. To minimize cells lost during the seeding procedure, we used the natural polymer fibrin to immobilize cells and to provide homogenous cells distribution in PLGA scaffolds. We evaluated in vitro chondrogenesis of rabbit articular chondrocytes in PLGA scaffolds using fibrin as cell transplantation matrix. Methods. PLGA scaffolds were soaked in chondrocytes-fibrin suspension (1 × 106cells/ scaffold) and polymerized by dropping thrombin-calcium chloride (CaCl ...
more infohttps://ukm.pure.elsevier.com/en/publications/fibrin-and-polylactic-co-glycolic-acid-hybrid-scaffold-promotes-e

Umbilical cord-derived mesenchymal stromal cells: predictive obstetric factors for cell proliferation and chondrogenic...Umbilical cord-derived mesenchymal stromal cells: predictive obstetric factors for cell proliferation and chondrogenic...

... aggrecans, and total type 2 collagen (Coll2T). Amongst the obstetric factors considered, birth weight, the number of amenorrhea ... Aggrecans (forward) 5′-TCG AGG ACA GCG AGG CC-3′ and (reverse) 5′-TCG AGG GTG TAG CGT GTA GAG A-3′; total type 2 collagen ( ... aggrecans and Coll2T) were assessed with SAS (SAS Institute, Brie Comte Robert, France) with a Pearson test or a Students t ... aggrecans, and Coll2T. Experiments were processed on 50 samples. Two samples could not be analyzed by RT-PCR because of the ...
more infohttps://stemcellres.biomedcentral.com/articles/10.1186/s13287-017-0609-z

YUHSpace: Comparative expression of matrix-associated genes and inflammatory cytokines-associated genes according to disc...YUHSpace: Comparative expression of matrix-associated genes and inflammatory cytokines-associated genes according to disc...

Adult ; Aged ; Aggrecans/genetics ; Aggrecans/metabolism ; Alkaline Phosphatase/genetics ; Alkaline Phosphatase/metabolism ; ...
more infohttps://ir.ymlib.yonsei.ac.kr/handle/22282913/93551?mode=simple

Buy Blackmores Glucosamine 1500 Tablets 90 at Health Chemist Online PharmacyBuy Blackmores Glucosamine 1500 Tablets 90 at Health Chemist Online Pharmacy

These structural units, called aggrecans, are important for the elasticity, resilience and shock-absorbing properties of ...
more infohttps://www.healthchemist.co.nz/natural-glucosamine/blackmores-glucosamine-1500-tablets-90-p2217007.html
  • Correlations were made between 27 obstetric factors and 8 biological indicators including doubling time at passage (P)1 and P2, the percentage of proteoglycans and collagens, and the relative transcriptional expression of Sox-9, aggrecans, and total type 2 collagen (Coll2T). (biomedcentral.com)
  • We have arbitrarily divided the ECM into two sections: (1) The collagen: PG complex itself, including the external membrane-bound PG, (e.g., aggrecans and decorin) and (2) The perturbations caused by the effects of the surrounding internal and external environment. (omicsonline.org)
  • Aggrecans" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (umassmed.edu)
  • This graph shows the total number of publications written about "Aggrecans" by people in this website by year, and whether "Aggrecans" was a major or minor topic of these publications. (umassmed.edu)
  • In particular, the tendency of the aggrecans to stick together under compressive force, then come apart moments after represents a major compression-dissipation system. (asknature.org)
  • The hallmark of the disease is the breakdown of articular cartilage by elevated proteinase activities that degrade major extracellular matrix molecules, aggrecans and collagens, but currently there are no effective treatments for OA, except joint replacement surgery. (grantome.com)
  • Aggrecans" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (ouhsc.edu)