Agar: A complex sulfated polymer of galactose units, extracted from Gelidium cartilagineum, Gracilaria confervoides, and related red algae. It is used as a gel in the preparation of solid culture media for microorganisms, as a bulk laxative, in making emulsions, and as a supporting medium for immunodiffusion and immunoelectrophoresis.Culture Media: Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.Microbial Sensitivity Tests: Any tests that demonstrate the relative efficacy of different chemotherapeutic agents against specific microorganisms (i.e., bacteria, fungi, viruses).Chromogenic Compounds: Colorless, endogenous or exogenous pigment precursors that may be transformed by biological mechanisms into colored compounds; used in biochemical assays and in diagnosis as indicators, especially in the form of enzyme substrates. Synonym: chromogens (not to be confused with pigment-synthesizing bacteria also called chromogens).Anti-Bacterial Agents: Substances that reduce the growth or reproduction of BACTERIA.Evaluation Studies as Topic: Studies determining the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies. For drugs and devices, CLINICAL TRIALS AS TOPIC; DRUG EVALUATION; and DRUG EVALUATION, PRECLINICAL are available.Colony Count, Microbial: Enumeration by direct count of viable, isolated bacterial, archaeal, or fungal CELLS or SPORES capable of growth on solid CULTURE MEDIA. The method is used routinely by environmental microbiologists for quantifying organisms in AIR; FOOD; and WATER; by clinicians for measuring patients' microbial load; and in antimicrobial drug testing.Bacteria: One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.Staphylococcus: A genus of gram-positive, facultatively anaerobic, coccoid bacteria. Its organisms occur singly, in pairs, and in tetrads and characteristically divide in more than one plane to form irregular clusters. Natural populations of Staphylococcus are found on the skin and mucous membranes of warm-blooded animals. Some species are opportunistic pathogens of humans and animals.Enterobacteriaceae: A family of gram-negative, facultatively anaerobic, rod-shaped bacteria that do not form endospores. Its organisms are distributed worldwide with some being saprophytes and others being plant and animal parasites. Many species are of considerable economic importance due to their pathogenic effects on agriculture and livestock.Blood: The body fluid that circulates in the vascular system (BLOOD VESSELS). Whole blood includes PLASMA and BLOOD CELLS.Food Microbiology: The presence of bacteria, viruses, and fungi in food and food products. This term is not restricted to pathogenic organisms: the presence of various non-pathogenic bacteria and fungi in cheeses and wines, for example, is included in this concept.Drug Resistance, Microbial: The ability of microorganisms, especially bacteria, to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).Feces: Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.Bacteria, AnaerobicPeptones: Derived proteins or mixtures of cleavage products produced by the partial hydrolysis of a native protein either by an acid or by an enzyme. Peptones are readily soluble in water, and are not precipitable by heat, by alkalis, or by saturation with ammonium sulfate. (Dorland, 28th ed)Staphylococcus aureus: Potentially pathogenic bacteria found in nasal membranes, skin, hair follicles, and perineum of warm-blooded animals. They may cause a wide range of infections and intoxications.Salmonella: A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria that utilizes citrate as a sole carbon source. It is pathogenic for humans, causing enteric fevers, gastroenteritis, and bacteremia. Food poisoning is the most common clinical manifestation. Organisms within this genus are separated on the basis of antigenic characteristics, sugar fermentation patterns, and bacteriophage susceptibility.Oxacillin: An antibiotic similar to FLUCLOXACILLIN used in resistant staphylococci infections.Streptococcus: A genus of gram-positive, coccoid bacteria whose organisms occur in pairs or chains. No endospores are produced. Many species exist as commensals or parasites on man or animals with some being highly pathogenic. A few species are saprophytes and occur in the natural environment.Candida: A genus of yeast-like mitosporic Saccharomycetales fungi characterized by producing yeast cells, mycelia, pseudomycelia, and blastophores. It is commonly part of the normal flora of the skin, mouth, intestinal tract, and vagina, but can cause a variety of infections, including CANDIDIASIS; ONYCHOMYCOSIS; vulvovaginal candidiasis (CANDIDIASIS, VULVOVAGINAL), and thrush (see CANDIDIASIS, ORAL). (From Dorland, 28th ed)Water Microbiology: The presence of bacteria, viruses, and fungi in water. This term is not restricted to pathogenic organisms.Cell Transformation, Neoplastic: Cell changes manifested by escape from control mechanisms, increased growth potential, alterations in the cell surface, karyotypic abnormalities, morphological and biochemical deviations from the norm, and other attributes conferring the ability to invade, metastasize, and kill.EsculinFamous PersonsAustralia: The smallest continent and an independent country, comprising six states and two territories. Its capital is Canberra.New Zealand: A group of islands in the southwest Pacific. Its capital is Wellington. It was discovered by the Dutch explorer Abel Tasman in 1642 and circumnavigated by Cook in 1769. Colonized in 1840 by the New Zealand Company, it became a British crown colony in 1840 until 1907 when colonial status was terminated. New Zealand is a partly anglicized form of the original Dutch name Nieuw Zeeland, new sea land, possibly with reference to the Dutch province of Zeeland. (From Webster's New Geographical Dictionary, 1988, p842 & Room, Brewer's Dictionary of Names, 1992, p378)HumanitiesEncyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)Personhood: The state or condition of being a human individual accorded moral and/or legal rights. Criteria to be used to determine this status are subject to debate, and range from the requirement of simply being a human organism to such requirements as that the individual be self-aware and capable of rational thought and moral agency.Glossitis: Inflammation of the tongue.Cosmetics: Substances intended to be applied to the human body for cleansing, beautifying, promoting attractiveness, or altering the appearance without affecting the body's structure or functions. Included in this definition are skin creams, lotions, perfumes, lipsticks, fingernail polishes, eye and facial makeup preparations, permanent waves, hair colors, toothpastes, and deodorants, as well as any material intended for use as a component of a cosmetic product. (U.S. Food & Drug Administration Center for Food Safety & Applied Nutrition Office of Cosmetics Fact Sheet (web page) Feb 1995)Laboratories: Facilities equipped to carry out investigative procedures.United States Food and Drug Administration: An agency of the PUBLIC HEALTH SERVICE concerned with the overall planning, promoting, and administering of programs pertaining to maintaining standards of quality of foods, drugs, therapeutic devices, etc.Clinical Laboratory Techniques: Techniques used to carry out clinical investigative procedures in the diagnosis and therapy of disease.Photography: Method of making images on a sensitized surface by exposure to light or other radiant energy.Animal Fins: Membranous appendage of fish and other aquatic organisms used for locomotion or balance.Growth Plate: The area between the EPIPHYSIS and the DIAPHYSIS within which bone growth occurs.Bone Plates: Implantable fracture fixation devices attached to bone fragments with screws to bridge the fracture gap and shield the fracture site from stress as bone heals. (UMDNS, 1999)Bacteriological Techniques: Techniques used in studying bacteria.Burn Units: Specialized hospital facilities which provide intensive care for burn patients.Government Programs: Programs and activities sponsored or administered by local, state, or national governments.Ontario: A province of Canada lying between the provinces of Manitoba and Quebec. Its capital is Toronto. It takes its name from Lake Ontario which is said to represent the Iroquois oniatariio, beautiful lake. (From Webster's New Geographical Dictionary, 1988, p892 & Room, Brewer's Dictionary of Names, 1992, p391)Rare Diseases: A large group of diseases which are characterized by a low prevalence in the population. They frequently are associated with problems in diagnosis and treatment.Canada: The largest country in North America, comprising 10 provinces and three territories. Its capital is Ottawa.Ross River virus: A species of ALPHAVIRUS associated with epidemic EXANTHEMA and polyarthritis in Australia.EncyclopediasDictionaries, MedicalDictionaries as Topic: Lists of words, usually in alphabetical order, giving information about form, pronunciation, etymology, grammar, and meaning.Molecular Sequence Annotation: The addition of descriptive information about the function or structure of a molecular sequence to its MOLECULAR SEQUENCE DATA record.Databases, Genetic: Databases devoted to knowledge about specific genes and gene products.

The Jun kinase 2 isoform is preferentially required for epidermal growth factor-induced transformation of human A549 lung carcinoma cells. (1/1701)

We have previously found that epidermal growth factor (EGF) mediates growth through the Jun N-terminal kinase/stress-activated kinase (JNK/SAPK) pathway in A549 human lung carcinoma cells. As observed here, EGF treatment also greatly enhances the tumorigenicity of A549 cells, suggesting an important role for JNK in cancer cell growth (F. Bost, R. McKay, N. Dean, and D. Mercola, J. Biol. Chem. 272:33422-33429, 1997). Several isoforms families of JNK, JNK1, JNK2, and JNK3, have been isolated; they arise from alternative splicing of three different genes and have distinct substrate binding properties. Here we have used specific phosphorothioate oligonucleotides targeted against the two major isoforms, JNK1 and JNK2, to discriminate their roles in EGF-induced transformation. Multiple antisense sequences have been screened, and two high-affinity and specific candidates have been identified. Antisense JNK1 eliminated steady-state mRNA and JNK1 protein expression with a 50% effective concentration (EC50) of <0.1 microM but did not alter JNK2 mRNA or protein levels. Conversely, antisense JNK2 specifically eliminated JNK2 steady-state mRNA and protein expression with an EC50 of 0.1 microM. Antisense JNK1 and antisense JNK2 inhibited by 40 and 70%, respectively, EGF-induced total JNK activity, whereas sense and scrambled-sequence control oligonucleotides had no effect. The elimination of mRNA, protein, and JNK activities lasted 48 and 72 h following a single Lipofectin treatment with antisense JNK1 and JNK2, respectively, indicating sufficient duration for examining the impact of specific elimination on the phenotype. Direct proliferation assays demonstrated that antisense JNK2 inhibited EGF-induced doubling of growth as well as the combination of active antisense oligonucleotides did. EGF treatment also induced colony formation in soft agar. This effect was completely inhibited by antisense JNK2 and combined-antisense treatment but not altered by antisense JNK1 alone. These results show that EGF doubles the proliferation (growth in soft agar as well as tumorigenicity in athymic mice) of A549 lung carcinoma cells and that the JNK2 isoform but not JNK1 is utilized for mediating the effects of EGF. This study represents the first demonstration of a cellular phenotype regulated by a JNK isoform family, JNK2.  (+info)

Effect of different lots of Mueller-Hinton agar on the interpretation of the gentamicin susceptibility of Pseudomonas aeruginosa. (2/1701)

Population distributions and quality control data for strains of Pseudomonas aeruginosa tested for gentamicin susceptibility on six lots of Mueller-Hinton agar were analyzed. The lots of agar were used in three University of Washington hospitals from April 1975 through October 1977. The analyses indicated that the performance of members of the P. aeruginosa populations in each hospital closely followed the performance of the quality control strain, P. aeruginosa ATCC 27853, when tested on each lot of Mueller-Hinton medium. The variability of zone diameters with the P. aeruginosa populations and the quality control strain indicated that a fixed indeterminate range (13 to 16 mm) of gentamicin susceptibility was not applicable to these organisms as it was with the Enterobacteriaceae. Variability in gentamicin susceptibility results was demonstrated in both minimal inhibitory concentration and disk diffusion tests when eight selected P. aeruginosa strains and the quality control strain were tested on each lot of medium. This variation in susceptibility to gentamicin was not related to the total Ca(2+), Mg(2+), or Zn(2+) content of each lot of medium. The data demonstrated that a moving indeterminate range of gentamicin susceptibility, 3 to 6 mm below the mean zone diameter of the quality control strain, was a suitable criterion for strains tested on a single medium lot. These results illustrate the importance of defining stringent performance standards for media used in the susceptibility testing of P. aeruginosa with gentamicin and other aminoglycoside antibiotics.  (+info)

Diffusion through agar blocks of finite dimensions: a theoretical analysis of three systems of practical significance in microbiology. (3/1701)

A number of experimental methods in biology depend on the kinetics of diffusion of a substance through a gel. This paper reviews the diffusion equations, gives the experimental limitations for some useful cases, and presents computer simulations for cases that cannot be treated analytically. While double diffusion is not considered, three single-diffusion situations are treated. (1) Systems for the study of chemotaxis in the gliding bacterium Myxococcus xanthus. Experimental designs used for this in many cases in the literature were inappropriate and mathematical analysis of these is presented. (2) The development of gradient plates. The time necessary for vertical diffusion to become substantially complete and before diffusion in the direction of the original slant has proceeded significantly is calculated. (3) The application to antimicrobial disk susceptibility tests. The basis of the measurement of antibiotic sensitivities with disks containing antimicrobial agents, as routinely used in clinical microbiological and testing laboratories, is analysed and the limitations are assessed and improvements suggested.  (+info)

Quantitative studies on competitive activities of skin bacteria growing on solid media. (4/1701)

Earlier quantitative investigations of antagonism between skin bacteria were based on the use of liquid cultures, but a more realistic model has now been devised, based on the use of the surfaces of solid media. Pure or mixed inocula were spread evenly over suitable agar media in Petri dishes marked out with a standard grid. Growth curves were constructed from viable counts of the surface bacteria after they had been removed from excised squares of the agar media and dispersed. The method was highly reproducible, and competitive interactions were revealed more clearly than in studies with liquid media. An antibiotic-producing strain of Staphylococcus epidermidis (S6+) readily suppressed strains of Micrococcus, Corynebacterium and Streptococcus species. However, a Staphylococcus aureus strain which was less sensitive to the antibiotic effect of S6+ interacted in a complex manner, depending on the absolute and relative size of the S6+ inoculum.  (+info)

Mad-overexpression down regulates the malignant growth and p53 mediated apoptosis in human hepatocellular carcinoma BEL-7404 cells. (5/1701)

Mad protein has been shown as an antagonist of c-Myc protein in some cell lines. The effect of Mad protein to the malignant phenotype of human hepatoma BEL-7404 cell line was investigated experimentally. An eukarryotic vector pCDNA III containing full ORF fragment of mad cDNA was transfected into targeted cells. Under G418 selection, stable Mad-overexpressed cells were cloned. Studies on the effect of Mad over-expression in cell proliferation and cell cycle revealed that cell morphology of the Mad-overexpressed BEL-7404-M1 cells was significantly different from the parent and control vector transfected cells. DNA synthesis, cell proliferation and anchorage-independent growth in soft-agar of the mad-transfected cells were partially inhibited in comparison to control cells. Flow Cytometry analysis indicated that mad over-expression might block more transfectant cells at G0/G1 phase, resulting in the retardation of cell proliferation. RT-PCR detected a marked inhibition of the expression of cdc25A, an important regulator gene of G0/G1 to S phase in cell cycle. It was also found that Mad protein overexpression could greatly suppress p53-mediated apoptosis in BEL-7404-M1 cells in the absence of serume. Thus, Mad proteins may function as a negative regulator antagonizing c-Myc activity in the control of cell growth and apoptosis in human hepatocellular carcinoma BEL-7404 cells.  (+info)

Use of Dorset egg medium for maintenance and transport of Neisseria meningitidis and Haemophilus influenzae type b. (6/1701)

Studies of bacterial meningitis are hampered by the inability to maintain the viability of etiological agents during transport to reference laboratories. The long-term survival rate of 20 isolates of Neisseria meningitidis and Haemophilus influenzae type b (Hib) on Dorset egg medium, supplemented Columbia agar base medium, chocolate agar, and Amies medium was compared with that on 70% GC agar (chocolate) transport medium. N. meningitidis isolates were also inoculated onto 5% horse blood agar, and Hib was inoculated onto Haemophilus test medium. All of the N. meningitidis isolates remained viable on Dorset egg medium for 21 days; viability on the other media was poor after only 7 days. Recovery rates of Hib isolates were similar on Dorset egg and Haemophilus test media (100% after 21 days) and significantly better than on the other media. Dorset egg medium is inexpensive and easy to make and may be invaluable for studies of bacterial meningitis in developing countries.  (+info)

Variation in Microbial Identification System accuracy for yeast identification depending on commercial source of Sabouraud dextrose agar. (7/1701)

The accuracy of the Microbial Identification System (MIS; MIDI, Inc. ) for identification of yeasts to the species level was compared by using 438 isolates grown on prepoured BBL Sabouraud dextrose agar (SDA) and prepoured Remel SDA. Correct identification was observed for 326 (74%) of the yeasts cultured on BBL SDA versus only 214 (49%) of yeasts grown on Remel SDA (P < 0.001). The commercial source of the SDA used in the MIS procedure significantly influences the system's accuracy.  (+info)

Visualization and modelling of the thermal inactivation of bacteria in a model food. (8/1701)

A large number of incidents of food poisoning have been linked to undercooked meat products. The use of mathematical modelling to describe heat transfer within foods, combined with data describing bacterial thermal inactivation, may prove useful in developing safer food products while minimizing thermal overprocessing. To examine this approach, cylindrical agar blocks containing immobilized bacteria (Salmonella typhimurium and Brochothrix thermosphacta) were used as a model system in this study. The agar cylinders were subjected to external conduction heating by immersion in a water bath. They were then incubated, sliced open, and examined by image analysis techniques for regions of no bacterial growth. A finite-difference scheme was used to model thermal conduction and the consequent bacterial inactivation. Bacterial inactivation rates were modelled with values for the time required to reduce bacterial number by 90% (D) and the temperature increase required to reduce D by 90% taken from the literature. Model simulation results agreed well with experimental results for both bacteria, demonstrating the utility of the technique.  (+info)

AIMS--To determine the best medium for the primary isolation of Helicobacter pylori. METHODS--Sixty six gastric mucosal biopsy specimens frozen in 1 ml Cysteine Albimi media with 20% glycerol from 22 histologically proven H pylori infected patients were cultured on brain heart infusion agar (BHIA) with 7% fresh whole defibrinated horse blood, egg yolk agar (EYA), Columbia blood agar-cyclodextrin agar (CBA-Cd), and commercial trypticase soy agar (TSA) supplemented with 5% sheep blood. RESULTS--Successful primary isolation of H pylori was 96% with BHIA, 78% with TSA, 64% for EYA, and 32% with CBA-Cd. Colonies appeared earlier on BHIA (4.7 +/- 0.1 days, 5.3 +/- 0.4 days, 5.3 +/- 0.4 days, and 7.1 +/- 0.9 days for BHIA, TSA, EYA, and CBA-Cd) and there were more colonies on BHIA than on CBA-Cd, EYA or TSA (599 +/- 88, 104 +/- 66, 260 +/- 107, and 358 +/- 89, respectively). CONCLUSIONS--Success of a medium for passage of isolates apparently does not reliably predict usefulness for primary isolation. ...
Tests for the presence of lipase and casease were negative, but B. pumilus did test positive for DNase. It does not hydrolyze starch or urea. It doesnt hydrolyze tryptophan either, and it cannot reduce sulfur. However, B. pumilus is motile. It cannot utilize citrate as a source of carbon, and it does not reduce nitrate. Being gram positive, B. pumilus does not grow on Eosin Methylene Blue agar, Hektoen Enteric agar, or MacConkey agar. It cannot decarboxylate arginine, lysine, or ornithine, and it does not deaminate the amino acid phenylalanine. B. pumilus does not grow on blood agar, or phenylethyl alcohol agar. It is not salt tolerant, so it will not grow on a Mannitol Salt agar or in a 6.5% NaCl broth. Infection of B. pumilus in humans is very rare, but it is possible. Its presence in rice has cause three cases of food poisoning. The symptoms included dizzyness, chills, stomach cramps, and diarrhea. There have also been three reported cases of cutaneous infection. The lesions that developed ...
Tests for the presence of lipase and casease were negative, but B. pumilus did test positive for DNase. It does not hydrolyze starch or urea. It doesnt hydrolyze tryptophan either, and it cannot reduce sulfur. However, B. pumilus is motile. It cannot utilize citrate as a source of carbon, and it does not reduce nitrate. Being gram positive, B. pumilus does not grow on Eosin Methylene Blue agar, Hektoen Enteric agar, or MacConkey agar. It cannot decarboxylate arginine, lysine, or ornithine, and it does not deaminate the amino acid phenylalanine. B. pumilus does not grow on blood agar, or phenylethyl alcohol agar. It is not salt tolerant, so it will not grow on a Mannitol Salt agar or in a 6.5% NaCl broth. Infection of B. pumilus in humans is very rare, but it is possible. Its presence in rice has cause three cases of food poisoning. The symptoms included dizzyness, chills, stomach cramps, and diarrhea. There have also been three reported cases of cutaneous infection. The lesions that developed ...
Chocolate agar (CHOC) or chocolate blood agar (CBA) - is a nonselective, enriched growth medium used for isolation of pathogenic bacteria. It is a variant of the blood agar plate, containing red blood cells that have been lysed by slowly heating to 80 °C. Chocolate agar is used for growing fastidious respiratory bacteria, such as Haemophilus influenzae and Neisseria meningitidis. In addition, some of these bacteria, most notably H. influenzae, need growth factors such as NAD (factor V) and hemin (factor X), which are inside red blood cells; thus, a prerequisite to growth for these bacteria is lysis of the red blood cells. The heat also inactivates enzymes which could otherwise degrade NAD. The agar is named for the color and contains no actual chocolate. Chocolate agar with the addition of bacitracin becomes selective, it is most critically, for the genus Haemophilus. Another variant of chocolate agar called Thayer-Martin agar contains an assortment of antibiotics which select for Neisseria ...
Mug Agar found in: Nutrient Agar w / MUG, 14mL, ECD MUG Agar, VRB MUG Agar, MUG Nutrient Agar, Plate Count MUG Agar, ECD MUG Agar, MacConkey MUG Agar, E...
High-throughput screens comparing growth rates of arrays of distinct micro-organism cultures on solid agar are useful, rapid methods of quantifying genetic interactions. Growth rate is an informative phenotype which can be estimated by measuring cell densities at one or more times after inoculation. Precise estimates can be made by inoculating cultures onto agar and capturing cell density frequently by plate-scanning or photography, especially throughout the exponential growth phase, and summarising growth with a simple dynamic model (e.g. the logistic growth model). In order to parametrize such a model, a robust image analysis tool capable of capturing a wide range of cell densities from plate photographs is required. Colonyzer is a collection of image analysis algorithms for automatic quantification of the size, granularity, colour and location of micro-organism cultures grown on solid agar. Colonyzer is uniquely sensitive to extremely low cell densities photographed after dilute liquid culture
Shop a large selection of E. coli Testing products and learn more about Thermo Scientific Blood Agar (TSA with Sheep Blood) Medium Blood Agar Blood Agar (TSA w/5% Sheep Blood);
Depending on the agar concentration, the film of liquid on the surface of the agar may be thick enough (?) to allow bacteria that have flagella to swim along the surface. Because the bacteria often move side-by-side in large groups this behaviour is called swarming. Often swarming on agar surfaces is facilitated by surfactants that the bacteria produce, which reduce the surface tension of the aqueous layer. Ive always assumed that bacteria living in soil and on other surfaces produce such surfactants as a way of getting surface-adsorbed nutrients into solution (thats how the surfactants we use in soaps and detergents do their job), but maybe surfactants are also advantageous for moving across surfaces with air-water interfaces, such as damp soil. The side-by-side cell orientation and movement may also be a consequence of surface-tension effects, as illustrated in this sketch ...
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The overall performance of SCITB agar plus the spot indole test for the detection of K. oxytoca was superior to that of MacConkey agar by McNemars test (P = 0.022). The sensitivities and specificities of the use of SCITB agar plus the spot indole test and MacConkey agar alone as a screening test were analyzed and are listed in Table 1 ...
BioAssay record AID 771708 submitted by ChEMBL: Antibacterial activity against Escherichia coli ATCC 25922 at 25 ug/disk after 24 hrs by agar diffusion assay.
Figure 3: Colonies from Sheeps blood agar plate. MICROBIOLOGY DIAGNOSIS:. Streptococcal pneumoniae is a Gram positive cocci arranged in chains and pairs. On blood agar plates, colonies are surrounded by a green alpha-hemolytic zone (due to production of pneumolysin which breaks hemoglobin into a green pigment). Optichin disks, which contain a quinine derivative, selectively inhibit the growth of S. pneumoniae on blood agar plates, and are used to differentiate pneumococcus from other viridans streptococci [1]. Susceptibility testing of the isolate revealed that it was sensitive to levofloxacin, erythromycin, tetracycline, sulfamethoxazol, chloramphenicol, and vancomycin. The MIC for penicillin and ceftriaxone were 0.023 µg/mL and 0.016 µg/mL, respectively.. This patients recurrent Streptococcus pneumoniae bacteremia, as well as his hyper-IgG, hypo-IgA and IgM prompted a search for an underlying immune disorder. PATIENT MANAGEMENT:. Upon identification of the Streptococcal pneumoniae, the ...
How is cycloserine-mannitol-blood agar , and cycloserine-cefoxitin agar abbreviated? M-CCA stands for cycloserine-mannitol-blood agar , and cycloserine-cefoxitin agar. M-CCA is defined as cycloserine-mannitol-blood agar , and cycloserine-cefoxitin agar rarely.
Specimen Collection: Consult listed references for information on specimen collection.(1,2,4,5,7-10) Infectious material should be submitted directly to the laboratory without delay and protected from excessive heat and cold. If there is to be a delay in processing, the specimen should be inoculated onto an appropriate transport media and refrigerated until inoculation. Consult the listed references for information regarding the processing and inoculation of specimens.(1,2,4,5,7-10) Method of Use: Allow media to warm to room temperature, and the agar surface to dry before inoculating. Inoculate and streak the specimen as soon as possible after collection. If the specimen to be cultured is on a swab, roll the swab over a small area of the agar surface. Streak for isolation with a sterile loop. Incubate plates in an inverted position. The caps of tubed media should be slightly loosened. Once inoculated, media should be protected from light and incubated aerobically at 25-35ºC. with increased ...
Specimen Collection: Consult listed references for information on specimen collection and processing of food, dairy, water samples, and other materials of sanitary significance. (1-8) Prior to inoculation, warm prepared media to room temperature. For melting bottled media: Autoclave at 121ºC. for one to three minutes or until melted. Alternatively, a covered, boiling waterbath (100ºC.) can be used. There should be enough water in the waterbath to reach the media line. A covered waterbath will help to reach and maintain the temperature. Heat in waterbath until melted. Spread Plate Method: 1. Prepare decimal dilutions in sterile diluent to obtain 30-300 CFU per plate. 2. Aseptically inoculate agar surface with 0.1ml of well mixed diluted sample. 3. Using a sterile spreader device, spread the dilution evenly over the surface of the agar. 4. Incubate plates aerobically for 48 +/- 2 hours at 35ºC. Pour Plate Method: 1. Melt agar by placing in a boiling waterbath until liquified. 2. Cool media to ...
Yeast And Mold Agar found in: M-Green Yeast and Mold Bottled Agar, Yeast Mold Agar (YM), Bottle Yeast Mold Agar 500G, Petrifilm™ Rapid Yeast and Mold Count..
Agar plates are commonly used in laboratories to grow bacteria for research purposes. The agar on the plate serves only as a medium to hold the bacteria, while added nutrients help the bacteria grow. Laboratories may use ready-made plates because they are consistent and faster than preparing plates from scratch.
Agar plate preparation. Young scientist pouring liquid agar growth medium into Petri dishes in a laminar flow cabinet. The agar will set into a gel when cool and is used as a medium for culturing micro-organisms such as bacteria and fungi. - Stock Image C028/7989
i have given an assignment to identify the fungi strain from about 50 of samples culture on agar plate. my lecturer have me to find the antibiotic and antifungal agent to add into the agar medium and redo the screening process of these samples ...
Hmmh... as far as I can tell that looks quite normal non-hemolytic growth typical to Bacillus species, though the angle and light are not ideal for spotting it. I am not sure what causes the darker coloration of the agar (Id call it deep red rather than black from what I see), but it is a common phenomenon possibly related to the use of other nutrients in the medium and to the change of pH or something. Bacteria break down and secrete various compounds, so I would not be surprised if some of those cause the darkening of the agar. Admittedly, I do not know the exact answer so if someone does, let us know. And the dark spot seems to be created by the administration of bacteriophages, as indicated in the text ...
If long term obsrvation is desired, a special slide needs to be made. Place masking tape on a slide, with a hold cut in the middle. Put a small drop of liquified NG agar on the slide and place a siliconized coverslip immediately on the agar. When the agar solidifies, remove the coverslip. Just prior to use, place a very small amount of E. coli on the agar, transfer a worm to the agar, and place a coverslip over the agar and the tape. Once the worm takes to eating the bacteria, it will settle down and can be readily observed for hours. ...
A blood agar plate is put in boiling water for about 1 hour until its colour changes from red to chocolate brown. Heating converts Haemoglobin into hematin ...
my competent cell grow on any agar plate - posted in Microbiology: I have been given a batch of XL1 blue cells. I made them competent using calcium chloride procedure. To test them, i plated them out on agar plate containing: Kanamycin, Tetracyclin, Ampicilin and control. After overnight incubation, there is bacteria colonies in all plates. These colonies are so dense we cant not see them separately.Since I have kept my original stock of cells before making them competent, I did the same t...
Introduction: Materials and Methods: 1.Using the swirled up Hay infusion from Lab 1 and 2, 100 ml of the hay infusion culture was added to 10mls of nutrient broth into a tube. This made a 1:100 dilution or 10^-2. Next 100microliters from that tube was placed into 10mls of nutrient broth to make a dilution of 1:10,000 or 10^-4. Next 100microliters from tube 4 was placed into 10mls of nutrient broth to make a dilution of 1:1,000,000 or 10^-6. Next 100microliters from tube 6 was placed into 10mls of nutrient broth to make a dilution of 1:10,000,000 or 10^-8. 2. Four nutrient agar plates were labeled. 10^-3, 10^-5,10^-7,10^-9. Another set of Agar plates with tetracycline were labeled with the same labels. 3. 100 microliters of the 10^-2 solution was placed on the surface of the 10^-3 agar plate and the 10^-3 agar plate with tetracycline. 100 microliters of the 10^-4 solution was placed on the surface of the 10^-5 agar plate and the 10^-5 agar plate with tetracycline. 100 microliters of the 10^-6 ...
Sigma-Aldrich offers Sigma-M7408, MacConkey Agar for your research needs. Find product specific information including CAS, MSDS, protocols and references.
Microbiological quality control of pharmaceutical water systems is of importance in ensuring that trends in contamination are detected and responded to. This is not least because water is a niche environment for many types of microorganisms and a vector for their transfer. Trending relates to actual microbial counts recorded, incidents and the types of species recovered. To facilitate species identification, microorganisms need to be subcultured from the isolation medium (R2A agar in Europe). Transfer onto the wrong media can result in the microorganism not growing. This paper describes research into three different media for subculturing: low nutrient (R2A); highly nutritious (TSA) and medium nutrient (R3A) and concludes that a higher recovery is obtained where R3A agar is used ...
We got back the agar that we made yesterday and we are supposed to interpret results. The different agars contain different substances which only allows some type of bacteria to grow on such as the MacConkey Agar (MAC) only allows gram-negative bacteria to grow. As for the agar that are in tubes, there was no bacteria on all of the samples except 1. I feel that it was because we did not smear it properly and hence we did not see any bacteria growth. We also get to use the microscope to see the different specimens. ...
The best way to grow bacteria on agar depends on the type of bacteria you want to grow. Agar is a substance extracted from red algae that forms a gel when mixed with water. It is a good medium for growing bacteria because it can be poured into forms such as a plate or test tube; it provides nutrients and moisture; and ...
PERFORMANCE OF THE ASSAY (Direct plate assay): Top agar in top agar tubes was melted by heating to 45 ± 2°C. The following solutions were successively added to 3 ml molten top agar: 0.1 mL of a fresh bacterial culture (10^9 cells/mL) of one of the tester strains, 0.1 mL of a dilution of the test item in DMSO and either 0.5 mL S9-mix (in case of activation assays) or 0.5 mL 0.1 M phosphate buffer (in case of non-activation assays). The ingredients were mixed on a Vortex and the content of the top agar tube was poured onto a selective agar plate. After solidification of the top agar, the plates were inverted and incubated in the dark at 37.0 ± 1.0 °C ...
agar polymerization - posted in General Lab Techniques: Hello everybody! I was wondering what could stop agar from polymerizing? I poured plates (5g agar, 0,9 g NaCL in 100ml H20) and left them to polymerize but in 24 hours they are still half liquid! What did I do wrong?
After meeting in art school on the outskirts of Paris, Clara and Armand came together to form techno pop duo Agar Agar. Hard synth bass lines bump behind Claras dark and sensual voice, at times giving their 80s era sound an almost angry intensity. Their first album Cardan was released in late 2016 and the pair is currently on tour in France.. ...
BioAssay record AID 1084736 submitted by ChEMBL: Antimicrobial activity against Escherichia coli 5933 after 24 hr by agar cup diffusion technique.
Ive got petri dishes, Ive got agar powder and dry malt extract. I did a prior run with ground up agar sticks into MEA, but when I put it into the petri dishes I had no idea  how much 
Nutrient agar medium for general bacteriological work. Supplied as 100cm3 lots in screw-cap bottles. This bulk pack is cost-effective, but the mediumtakes longer to melt so there is some risk of caramelisation, or contamination if not all used immediately. Pack size 2.
The noble agar assay is based on the principle that the cancer cells are anchorage-independent and do not show contact inhibition. This type of soft agar assay can give insight into two important properties of a subset of cancer cells. Firstly, the tumorigenic efficiency of cancer cells can be determined based on number of colonies that emerge. This is based on the ability of cells to grow unattached and to remain suspended in agar. Secondly, the self-renewal properties of cancer cells can be assessed based on the types of progeny produced in noble agar.. ...
Triple Sugar Iron Agar and Its Use and Interpretation see Difco Manual, 9th Ed, (1953) pp. 166-168 (Table on p. 161.) INTRODUCTION: Triple Sugar Iron medium is a differential medium that can distinguish between a number of Gram-negative enteric bacteria based on their physiological ability (or lack thereof) to: a. metabolize lactose and/or sucrose…
Bd bbl bcye selective agar wccvc is a highly selective medium consisting of bd bbl. Mold agar slants with chloramphenicol brand bd cheap price after look at the price. Uk, wed like to know more about.
Place a coffee filter in a strainer and hang the strainer over the opening of the clear bowl.. Slowly pour the broth into the filter, making sure that the broth doesnt overflow from the sides of the filter. If the filter becomes clogged with particles, remove the used filter, replace with a clean filter and continue pouring the broth.. Remove the strainer and filter from the top of the bowl and observe whether the broth appears clear (i.e., there are no suspended particles in the liquid). If particles are floating in the liquid, repeat Steps 1 and 2 using a second clear bowl and a fresh coffee filter.. Place the pot on the stove and pour the clear broth into the pot.. Add 16 ounces of distilled water to the pot containing the broth.. Turn on the stove and adjust the heat to medium-low. Heat the broth to a simmer (i.e., small bubbles appear near the side of the pot).. Add 1 teaspoon of the agar-agar powder to the simmering broth (approximately 2.5 grams) and stir the mixture until the powder is ...
At 11:17 AM 5/17/01 -0800, Karen Larison wrote: ,We first embed them in a ,melted agar-sucrose matrix, which allows us to orient them very ,precisely within the block for serial sectioning. Karen, What is the formula for the agar and sucrose? I am interested in trying this. Do you need to let the tissues infiltrate in the agar for a while? Connie McManus We then let the ,block solidify and then sink it in in sucrose before sectioning. The ,nice thing about these agar blocks is that they ribbon nicely. You ,can cut a series of sections with the antiroll plate, then use a ,brush to straighten them out, and then pick them up. Using this ,method, we are better able to position these tiny sections on a ,slide, we are less likely to experience leaping sections, and we ,can get 70-100 sections on one slide in very short order! , ,We also use Tissue Freezing Medium (made by TBS, available from ,Fisher Scientific) to adhere our agar block to the chuck, and find ,that this curls less, and appears to ...
L-Broth Agar is a nutritionally rich medium originally developed for growth and maintenance of recombinant E. coli strains. MP Biomedicals offers L-Broth Agar medium in an encapsulated format for convenience and ease of use.
PRE-INOCULATION: The Petri dish and agar are sterilised to kill any unwanted bacteria. This is achieved by passing the inoculation loop through a flame so that it becomes sterile. INOCULATION: The now sterile loop is dipped in a suspension of bacteria which are transferred to the agar (made of carbohydrates). The lid of the Petri dish is replaced quickly to prevent microbes from the air entering. POST-INOCULATION: The Petri dish is sealed with tape to prevent microbes from the air entering and contaminating the culture. It is important to not seal the dish all the way to ensure oxygen can enter. Otherwise harmful anaerobic bacteria would grow. The Petri dish is then incubated at 25oC max to allow the bacteria to grow. Above 25oC pathogens might grow instead. ...
1.2 Use half-strength (nutrient and agar) tryptic soy agar as the inoculum vehicle for bacteria and half-strength potato dextrose agar as the inoculum vehicle for mold conidia. Use of half-strength agars may reduce undue neutralization of an antimicrobial due to excessive organic load. ...
Each year, many people die of nosocominal infections - infections that the patients acquired in a hospital. However, these infections would be preventable in large part through better hygiene programs. Diseases caused by multi-resistant pathogens are especially critical because therapy options are very limited in these cases. But actually, what are pathogens exactly and where do they come from? In order to answer these questions, we start the teaching module with the topic "Microorganisms in our Environment". In this part, we explain which germs we encounter every day, and how be can best protect ourselves from them. In order to demonstrate how many and which microorganisms are our steady companions, we conduct an experiment with the students. A pair of students received 3 agar plates: one with regular LB agar, one plate with agar supplemented by an antibiotic, and one selective agar plate for yeasts and fungi. Equipped with the plates, the students wander through their school and take contact ...
Does anyone have any experience, tips, or suggestions for a multispore agar innoculation? I have spore prints, and I have read some of the documents regarding techniques. Any advice though would
Agar high purity, low ionic content, low gel strenght; CAS Number: 9002-18-0; EC Number: 232-658-1; Synonym: Agar-agar, Gum agar; Linear Formula: (C12H18O9)n; find Millipore-56763 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich.
Plant Agar is a multi-purpose agar useful in plant cell and tissue culture to be used at a minimum concentration of 5.5 g/l. Derived from red algae.
i had kept the rest of the almond milk in the fridge this whole time and adding the cold milk to the thickened heated agar milk mixture instantly gelled it! so it got clumpy! this is what i was trying hard to avoid in the first place, ugh! i tried beating it at high for a longer time but it still was clumpy. (the clumps taste more like clumps of agar than clumps of extra thick chocolatey stuff, so kinda not so great) oh well ...
An apparatus for automatically transferring a desired one of bacterial colonies grown in a medium in a culture Petri dish onto a medium in a test Petri dish or a test tube by using a bacterial colony pick-up/transfer element of the disposable type or a bacterial colony pick-up/transfer element of the reuse type and by using various mechanisms controlled by a computer.
Mineralstoff, Spurenelement, Vitamin, Magnesium, Calcium, Kalium, Eisen, Kupfer, Chrom, Bor, Molybd n, Selen, Mangan, Chromium, Potassium, Manganese, Selenium, Copper, Boron, Molybdenum, Zink, Zinc, Iron, Iodine, Jod, Trace Mins, Minerals,
They are known in Spanish as Dulce de Agar (Agar sweets). Agar-agar is an allowed nonorganic/nonsynthetic additive used as a ... Agar (pronounced /ˈeɪɡɑːr/, US: /ˈɑːɡər/) or agar-agar is a jelly-like substance, obtained from algae.[1] ... The word "agar" comes from agar-agar, the Malay name for red algae (Gigartina, Gracilaria) from which the jelly is produced.[14 ... Main article: Agar plate. An agar plate or Petri dish is used to provide a growth medium using a mix of agar and other ...
In the 17th century, it was discovered that freezing tokoroten would result in a stable and dry product known as kanten (agar ... Armisen, Rafael; Galatas, Fernando (1987). "PRODUCTION, PROPERTIES AND USES OF AGAR". In McHugh, Dennis J. Production and ... Shimamura, Natsu (August 4, 2010). "Agar". The Tokyo Foundation. Retrieved 19 December 2016. Stephen, Allistair M. (1995). Food ...
Agar • Aggregatibacter actinomycetemcomitans • Aim toothpaste • Akers' clasp • Alberta Dental Association and College • Alfred ...
Nita Agar; Antony R. Young (2005). "Review: Melanogenesis: a photoprotective response to DNA damage?". Mutation Research/ ...
Agar, N.; Young A. R. (2005). "Melanogenesis: a photoprotective response to DNA damage?". Mutation Research. 571 (1-2): 121-132 ...
Revised 2005 February Currow D; Agar MR; Abernethy AP (2011). "Tackling the Challenges of Clinical Trials in Palliative Care". ...
Agar, Jesse. "Tragedy of the Commons │ The Problem with Open Access". Youtube. This Place (youtube channel). Retrieved Jun 9, ...
The procedure starts with plating 0.1 ml of dilutions of heat-treated soil suspensions on glucose mineral base agar: 10g Glc; ... 1g (NH4)2SO4 or KNO3; 0.8g K2HPO4; 0.2g KH2PO4; 0.5g MgSO4·7H2O; 0.05g CaSO4·7H2O; 0.01g FeSO4·7H2O; 12g agar; distilled water ...
Agar, M; de Rooij, SE; Meagher, D; Trabucchi, M; MacLullich, AM (December 2013). "Consensus and variations in opinions on ...
Ben Agar. 1643. Hirschfeld, Heather Anne (17 January 2018). "Joint Enterprises: Collaborative Drama and the ...
Seth Agar. (David Lambert) 1768- 70. George Palmes. (Thomas Williamson) 1771-2. Sir Thomas Gascoigne, Bart. (Thomas Johnson) ...
Agar, R.; Asker, A. H.; Hanif, M. (1987), "Surface effects and tectonic setting of the 13 December 1982 North Yemen earthquake ...
Agar, Charles; Eveland, Jennifer (2005). Frommer's Southeast Asia. John Wiley & Sons. p. 158. ISBN 978-0-7645-7829-8. Retrieved ...
The organism is usually cultured in Burkholderia cepacia agar (BC agar) which contains crystal violet and bile salts to inhibit ... "OFPBL agar". Remel Technical Manual. Lenexa, Kan: Remel. 1997. Avgeri SG; Matthaiou DK; Dimopoulos G; Grammatikos AP; Falagas ... Alternatively, oxidation-fermentation polymyxin-bacitracin-lactose (OFPBL) agar can be used. OFPBL contains polymyxin (which ... which helps to distinguish it from other organisms that may grow on OFPBL agar, such as Candida species, Pseudomonas ...
"1999 - A Visit to Edgar". Bob Agar. 2000-04-25. Retrieved 2008-05-26. "1999 - A Visit to Edgar". Bob Agar. 2000-04-25. ... ISBN 978-0-9681973-0-1. "1999 - A Visit to Edgar". Bob Agar. 2000-04-25. Retrieved 2008-05-26. " ...
"Captain Augustus Agar, VC (Obituary)". The Times. 1 January 1969. p. 10 column F. Agar 1961, p. 147. Agar 1961, p. 149. Agar ... Agar 1961, p. 153. Agar 1961, p. 154. Agar 1961, p. 155. Hayward 1994, pp. 37-41. Christopher J White and Peter Robinson. "RFA ... Augustus Agar was chosen to lead the operation. Agar was an officer of the Royal Navy, who had been awarded the Victoria Cross ... Time was of the essence and Agar regretted that for the sake of secrecy he could not tell the workmen exactly what the ships ...
Agar, Charles (2009). Insiders' Guide to Colorado's Mountain. Guilford, CT: Globe Pequot Press. p. 137. ISBN 978-0-7627-5342-0 ...
Agar, Jon (2004). Constant Touch: a Global History of the Mobile Phone. Cambridge: Icon. ISBN 978-1840465419. Farley, Tom (2007 ...
Agar, Nicholas (2001). "Recent defenses of biocentrism". Life's intrinsic value: science, ethics, and nature. Columbia ...
Agar, Nicholas (2006). "Designer Babies: Ethical Considerations". ActionBioscience.org. Pang, Ronald T.K (January 2016). " ...
Agar, Jon (2003). Constant Touch: A Global History of the Mobile Phone. Cambridge: Icon. Carey, James (1989). Communication as ...
com Agar Scientific. 2015. Retrieved August 25, 2015. "AQUADAG E - Water Based Colloidal Graphite Resistance Coating" (PDF). ...
Agar, Charles (2006). Frommer's Vietnam. John Wiley and Sons. p. 146. ISBN 0-7645-9676-4. Official website of Đồng Xuân Market ...
BJP retains Agar; defeats Congress by over 27,000 votes Janpratinidhi Sitting and previous MLAs from Agar (SC) Assembly ... Parmar is a member of the Madhya Pradesh Legislative Assembly from the Agar constituency in Agar Malwa district. ...
Agar, Herbert (1960). The Saving Remnant: An Account Of Survival. New York: The Viking Press. p. 136. "Search Results - Record ... Agar 1960, p. 137. Verbannte - Rare Book and Special Collections - LibGuides at University of Newcastle Library[dead link] " ... The Navemar/Opinion of the Court at Wikisource Agar 1960, p. 136. Seitel, Becky. "The Crossing". Jenny Fried Cohen. Birmingham ...
They are known in Spanish as Dulce de Agar (Agar sweets). Agar-agar is an allowed nonorganic/nonsynthetic additive used as a ... Agar (pronounced /ˈeɪɡɑːr/, US: /ˈɑːɡər/) or agar-agar is a jelly-like substance, obtained from algae.[1] ... The word "agar" comes from agar-agar, the Malay name for red algae (Gigartina, Gracilaria) from which the jelly is produced.[14 ... Main article: Agar plate. An agar plate or Petri dish is used to provide a growth medium using a mix of agar and other ...
Nicholas Agar (born 1965) is a professor of ethics at the Victoria University of Wellington.[1] Agar has a BA from the ... Agars personal webpage. *Review of Agar, N: 2013, Truly Human Enhancement: A Philosophical Defense of Limits Med Health Care ... Agar is a member of the New Zealand Governments Environmental Risk Management Authority Ethics Advisory Council.[3] He was ... Agars main research interests are in the ethics of the new genetics. He has also published on personhood theory, environmental ...
Alternative form of agar-agar. Further reading[edit]. *"agar" in Diccionario de la lengua española, Vigésima tercera edición, ... agar. n-agar. hagar. t-agar. Note: Some of these forms may be hypothetical. Not every. possible mutated form of every word ... Synonym of agar-agar. Ido[edit]. Etymology[edit]. Borrowed from Esperanto agi, French agir, German agieren, Italian agire, ... agar. unchanged. n-agar. Note: Some of these forms may be hypothetical. Not every. possible mutated form of every word actually ...
Agar (sl); Agar (tl); Agaragar (hu); Агар-агар (tg); Agar-agar (id); agar-agar (pl); അഗർ (ml); Agar (sh); Agar-agar (oc); Agar ... agar-agar, kanten (it); Agar agar, Agar, 9002-18-0, E406, E 406, E-406 (fr); Agar-agar (et); Agar agar, Agarófita, Agár, Agar- ... Agar, Agarose, Agarosa (id); Agar agar (ca); Agar (ml); Agar agar, E406, Agar-Agar, Agaragar, Agarose (nl); Agar Agar (tr); ... Agar (la); अगर (hi); 洋菜 (zh-tw); Agar (fi); 洋菜 (zh-hans); agar-agar (ca); agar (cs); Agar-agar (tg-latn); agar agar (it); ágar- ...
Auclair JR, Salisbury JP, Johnson JL, Petsko GA, Ringe D, Bosco DA, Agar NY, Santagata S, Durham HD, Agar JN. ... Basu SS, Randall EC, Regan MS, Lopez BGC, Clark AR, Schmitt ND, Agar JN, Dillon DA, Agar NYR. ... Rawlins CM, Salisbury JP, Feldman DR, Isim S, Agar NY, Luther E, Agar JN. ... Agar JC, Cao Y, Naul B, Pandya S, van der Walt S, Luo AI, Maher JT, Balke N, Jesse S, Kalinin SV, Vasudevan RK, Martin LW. ...
Gonorrhea Laboratory Information Agar Dilution Antimicrobial Susceptibility Testing ... Agar-Dilution Susceptibility Test Procedure. *Grow pure cultures of isolates to be tested on chocolate agar at 35 C to 36.5 C ... Use pure cultures on chocolate agar to prepare the inoculum. Do not use the first subculture from a frozen culture; subculture ...
Note: add 3 g agar to Difcos dehydrated starch agar.. Hypertext Source: Bacteriological Analytical Manual, 8th Edition, ... Heat to dissolve agar in 500 ml water. Dissolve starch in 250 ml water. Combine and dilute to 1 liter. Autoclave 15 min at 121° ...
Tryptic soy agar, tryptic soy agar blood base, or trypticase soy agar [soybean-casein digest agar (M152)] may be used as the ... Commercially available sheep blood agar plates are satisfactory. Blood agar base is available from Difco, BBL, and Oxoid. ... Autoclave 15 min at 121°C. Cool to 50°C. Add 5 ml defibrinated sheep red blood cells to 100 ml melted agar. Mix and pour 20 ml ...
Sabouraud Agar Commonly used for fungus cultures[11] Thayer-Martin Agar Chocolate agar designed to isolate gram-negative ... XLD Agar Sources. There are different kinds of oceanic plant life from which agar can be extracted. The following are some of ... Neomycin Agar A kind of blood agar, which is selective Useful for the isolation of S. pyogenes S. and agalactiae in suspected ... Types of laboratory agar. A variety of agar types have been developed for various purposes. ...
Download this Agar Plates With Bacterias photo now. And search more of the webs best library of royalty-free stock images from ... Agar plates with bacterias - Stock image. .... Laboratory, Achievement, Agar Jelly, Analyzing, Bacterium. ...
... Kimberly Walker walker at umbi.umd.edu Fri Mar 14 17:57:44 EST 1997 *Previous message: transient transfection ...
Agar-Agar at Agar-Agar.org "Agar-Agar". Botanical.com. Retrieved 22 January 2017. "III: Properties, Manufacture, and ... Agar (pronounced /ˈeɪɡɑːr/, US: /ˈɑːɡər/) or agar-agar is a jelly-like substance, obtained from algae. Agar forms the ... They are known in Spanish as Dulce de Agar (Agar sweets) Agar-agar is an allowed nonorganic/nonsynthetic additive used as a ... The word "agar" comes from agar-agar, the Malay name for red algae (Gigartina, Gracilaria) from which the jelly is produced. It ...
Learn more about Agar uses, effectiveness, possible side effects, interactions, dosage, user ratings and products that contain ... Medications taken by mouth (Oral drugs) interacts with AGAR. Agar is a thick gel. Agar might stick to some medications in the ... AGAR. OTHER NAME(S): Agar-Agar, Agarose, Agarose Gel, Agaropectin, Agarweed, Algue de Java, Chinese Gelatin, Colle du Japon, ... In Japan agar is called "kanten," and it is the main ingredient in "the kanten plan" or "the kanten diet.". People use agar for ...
Baird-Parker Agar) which is used for the selective isolation and enumeration of coagulase-positive %3Cem class=%22MMM--txt_ ... Medium for the preparation of egg-tellurite-glycine-pyruvate agar ( ... Medium for the preparation of egg-tellurite-glycine-pyruvate agar (Baird-Parker Agar) which is used for the selective isolation ...
How Agar Art Is Made Sunnybrook Health Sciences Centre Written by Sybil Millar, Communications Advisor for the Ross Tilley Burn ...
Agar comes from seaweed that grows on the rocky areas of tidal waters of Japan, China and Sri... ... Agar has been used in Asian cooking for hundreds of years. ... "agar nutritional value,agar diet benefits,agar uses ... Agar comes from seaweed that grows on the rocky areas of tidal waters of Japan, China and Sri Lanka. Agar is white and semi- ... Agar contains no cholesterol.. Benefits. Agar acts as an appetite suppressant; it becomes gelatinous when wet, swells and gives ...
... M Des kayamicdes at hotmail.com Tue Nov 29 09:22:59 EST 2005 *Previous message: Cloning 101 ... agar, seeded with the indicator bacteria). We also deposed a spot (10 uL) directly on the agar. After incubation, absolutely no ... We supposed that the proteins agglomerate together and remained trapped in the agar matrix, but adding Tween in the fractions ...
The word agar comes from the Malay word agar-agar (meaning "jelly"). It is also known as kanten or agal-agal (Ceylon agar). ... EMB agar causes E. coli colonies to have a metallic green sheen, and MSA (Mannitol Salt Agar) turns yellow in the presence of ... Agar is typically sold as packaged strips of washed and dried seaweed, or in powdered form. Raw agar is white and semi- ... An agar plate is a sterile petri dish that contains agar plus nutrients (growth media), used to culture microorganisms. ...
Endo agar (also called Endos medium) is a microbiological growth medium with a faint pink colour. Originally developed for the ... agar Mondofacto Becton, Dickinson and Company, [1], 2006 EMD Chemicals, "Archived copy". Archived from the original on 2009-04- ... Endo agar typically contains (w/v): 1.0 % peptone 0.25 % dipotassium hydrogen phosphate (K2HPO4) 1.0 % lactose 0.33 % anhydrous ...
EWGs Skin Deep® database gives you practical solutions to protect yourself and your family from everyday exposures to chemicals in personal care products.
Make research projects and school reports about agar easy with credible articles from our FREE, online encyclopedia and ... Nutrient agar consists of a broth made from beef extract or blood that is gelled with agar and used for the cultivation of ... When heated with water and subsequently cooled to about 45°C, agar readily forms a gel (jelly); an agar gel supplemented with ... When heated with water and subsequently cooled to about 45°C, agar readily forms a gel (jelly); an agar gel supplemented with ...
Agar Art winner Neurons. Posted October 22, 2015 11:53:53 The winning entry in the 2015 ASM Agar Art competition: Neurons by ... The challenge was to create a piece of art using microbes as the paint and agar as the canvas. ...
When should I use broth, agar plates, agar slants or agar deeps in culturing bacteria? ... Agar slants and deeps are mainly used to store bacteria for a long time (months, years). Broth is used when you have 1 single, ... Agar plates when you want to count colonies, or when you start with a mixed community (with multiple species of bacteria), and ... Because Agar is the suitable place for the bacteria to live and reproduce.It is a cultural media for bacteria. ...
Privacy Policy - Copyright Policies Tree of Life design and icons copyright © 1995-2004 Tree of Life Project. All rights reserved.. ...
Be sure to call ahead with Kristin Agar to book an appointment. ... Kristin Agar, LCSW is a Social Worker in Little Rock, AR. She ... Kristin Agar, LCSW is a Social Worker in Little Rock, AR. She is accepting new patients. Be sure to call ahead with Kristin ... Kristin Agar, LCSW is a Social Worker in Little Rock, AR. She is accepting new patients. Be sure to call ahead with Kristin ...
  • Grow pure cultures of isolates to be tested on chocolate agar at 35 C to 36.5 C for 16 to 18 h in a carbon dioxide-enriched (5%) atmosphere. (cdc.gov)
  • Use pure cultures on chocolate agar to prepare the inoculum. (cdc.gov)
  • Chocolate agar, however, does not reveal hemolysis data, so species differentiation among the members of Haemophilus must be performed in another manner. (sciencebuddies.org)
  • Chapter 2, to profile the top manufacturers of Agar, with price, sales, revenue and global market share of Agar in 2017 and 2018. (openpr.com)
  • Agar is actually the resulting mixture of two components: the linear polysaccharide agarose, and a heterogeneous mixture of smaller molecules called agaropectin . (wikipedia.org)
  • Agar is an unbranched polysaccharide extracted from the cell walls of some species of red algae or seaweed and having major economic importance. (newworldencyclopedia.org)
  • Agar is a polysaccharide complex obtained through bleaching and hot water extraction of agarocytes from the red alga Rhodophyceae, found in the Pacific and Indian Oceans and in the Sea of Japan. (thomassci.com)
  • Agar forms the supporting structure in the cell walls of certain species of algae, and which is released on boiling. (wikipedia.org)
  • Furthermore, as a derivative of marine algae, agar is speculated to provide improved moisture-retention, which may help to reduce skin dryness. (lorealparisusa.com)
  • Chemically, agar is a polymer made up of subunits of the sugar galactose, and is a component of the cell walls of several species of red algae that are usually harvested in eastern Asia and California. (sciencebuddies.org)
  • Agar consists of a mixture of two polysaccharides: agarose and agaropectin, with agarose making up about 70% of the mixture. (wikipedia.org)
  • YES Agar is a nutritionally rich medium for the growth of S. pombe, containing dextrose, yeast extract and agar. (mpbio.com)
  • The yeast did grow but it is rather clumpy with the rough agar surface and not easy to find a clean colony. (homebrewersassociation.org)
  • When I used baby food jars, I just streaked with a loop to get yeast "lawn" and then poured sterile wort from another jar onto the agar to resurrect the yeast. (homebrewersassociation.org)
  • Laboratories participate in the AGAR surveys by providing laboratory data and isolates to the AGAR coordinating laboratories. (safetyandquality.gov.au)
  • A total of 28 isolates were isolated with Cetrimide agar from raw water samples and identified using biochemical tests and commercial identification kits. (scirp.org)
  • We describe a newly developed agar-based method employing disk diffusion assay to test the susceptibility of 47 isolates of dermatophytes against 8 antifungals. (asm.org)
  • In laboratories, The agar gel is usually placed in a Petri Dish (or Petri Plate), and inoculated with one or more bacterial or fungal samples. (conservapedia.com)
  • I would like to know what is the reason behind researchers using 1.5% agar plate? (protocol-online.org)
  • however, as it can ferment the glucose in the media, it appears bright pink on this V.R.B.G. agar plate. (asm.org)
  • Specifications for bacteriological grade agar include good clarity, controlled gelation temperature, controlled melting temperature, good diffusion characteristics, absence of toxic bacterial inhibitors and relative absence of metabolically useful minerals and compounds. (sciencebuddies.org)
  • Inspired by observing bacterial growth in agar and by the transfer of information through simple agar simulations, the cognitive simulation of Noble Ape (originally developed in 1996) has defined itself as both a philosophical simulation tool and a processor metric. (igi-global.com)
  • There are thousands of different recipes but the one we use in Mediamatic is Potato Dextrose Agar ( abbreviation PDA), is also the one I tried first so I can report here the recipe I used. (mediamatic.net)
  • However, Potato dextrose agar 0 was used for Candida albicans and the incubation conditions of 28[degrees]C and 48 hours of incubation were 0 used. (thefreedictionary.com)
  • Sterilized Potato Dextrose agar was poured into nine Petri dishes and allowed to solidify at room temperature. (thefreedictionary.com)
  • Organisms were subcultured on potato dextrose agar (PDA) or oatmeal agar (for T. rubrum ) at 30°C for 4 to 15 days. (asm.org)
  • aeruginosa showed that the growth of accompanying flora may be suppressed by adding 375-μg/mL BKC into Cetrimide agar and incubating at an elevated temperature of 42°C. (scirp.org)
  • According to QuiteHealthy.com, a 1-ounce serving size, or 28 grams, of agar contains 0.9 grams of total fat, 87 calories, 22.93 grams of carbohydrate and 1.76 grams of protein. (livestrong.com)
  • Use cubes of agar to investigate how size impacts diffusion. (exploratorium.edu)
  • By infusing cubes of agar with a pH indicator, and then soaking the treated cubes in vinegar, you can model how diffusion occurs in cells. (exploratorium.edu)
  • Measurement of dye diffusion in agar gel by use of low-coherence interferometry. (nih.gov)
  • In the current study, we optimized an agar-based disk diffusion method to determine the susceptibility of dermatophytes to various antifungals. (asm.org)
  • Young scientist pouring liquid agar growth medium into Petri dishes in a laminar flow cabinet. (sciencephoto.com)
  • Though frequently treated and described as a vegan gelatin substitute in other parts of the world, win (วุ้น) or agar (also "agar agar") is used to make snacks/desserts throughout East and Southeast Asia where people-let me know if I'm wrong on this-consume, because they like it, not because they want to eat JELL-O but have to settle for something similar. (seriouseats.com)
  • Agar-Agar (Gelatin Substitute) is vegan, kosher, and gluten-free. (naturesflavors.com)
  • Beginning in the late 19th century, agar began to be used heavily as a solid medium for growing various microbes. (wikipedia.org)
  • In 1882, Fannie Hesse came up the creative idea of using agar as a solid medium, after combining her knowledge of her husband's work with her knowledge of cooking. (conservapedia.com)
  • A sample of the blended formulation is used to prepare agar medium. (mpbio.com)
  • It is possible to make different types of medium with agar with very simple and quick preparation, in this article I will describe some of the more common recipes. (mediamatic.net)
  • Agar Leukocyte Conditioned Medium is a source of colony-stimulating factors for assays of hematopoietic progenitor cells from light density fractions of human bone marrow and peripheral blood. (stemcell.com)
  • Agar Leukocyte Conditioned Medium is prepared using normal human peripheral blood leukocytes in an agar-containing medium. (stemcell.com)
  • does one have to add extra medium during the growing time or will they survive with the nutrients and the moisture in the agar? (protocol-online.org)
  • When suspended in a liquid medium and heated to 212°F (100°C), the agar dissolves. (thefreedictionary.com)
  • Add 7.5 g Bacto agar to empty 1 L bottles, aliquot 500 ml of medium to each bottle. (openwetware.org)
  • General purpose relatively non-selective agar medium, containing two peptones, which will support the growth of a wide variety of organisms. (lamotte.com)
  • I am not sure what causes the darker coloration of the agar (I'd call it deep red rather than black from what I see), but it is a common phenomenon possibly related to the use of other nutrients in the medium and to the change of pH or something. (biology-online.org)
  • MUG Violet Red Bile Agar is therefore recommended for the specific detection of E. coli (1, 2-4). (thomassci.com)
  • CRITERION™ Violet Red Bile Agar with MUG is primarily for use in the detection of Escherichia coli and total coliforms in food and dairy products. (thomassci.com)
  • add 3 g agar to Difco's dehydrated starch agar. (fda.gov)
  • Tryptic Soy Agar is used for the preparation and maintenance of test strains used in growth promotion tests, suitability of the counting methods and as negative controls as described in the Harmonized USP/EP/JP. (neogen.com)
  • Tryptic Soy Agar is recommended in multiple water & wastewater applications, and numerous standard methods for food testing. (lamotte.com)
  • Heat to dissolve agar in 500 ml water. (fda.gov)
  • Heat with agitation to dissolve agar. (fda.gov)
  • The challenge was to create a piece of art using microbes as the paint and agar as the canvas. (abc.net.au)
  • Research For Market in this report on Global Agar Market will keep you apprised in this CAGR growing market by letting you know in-depth how the key players and brands are driving the market in the forecast period of 2019-2025. (openpr.com)
  • Chapter 4, the Agar breakdown data are shown at the regional level, to show the sales, revenue and growth by regions, from 2014 to 2019. (openpr.com)
  • Boettcher, P.A., Agar, E. , Dennison, C., Kumbur, E.C. (2016) "Modeling of ion crossover in vanadium redox flow batteries: A computationally-efficient lumped parameter approach for extended cycling," Journal of the Electrochemical Society 163:1 pp. (uml.edu)
  • Agar exhibits hysteresis , melting at 85 °C (358 K, 185 °F) and solidifying from 32-40 °C (305-313 K, 90-104 °F). This property lends a suitable balance between easy melting and good gel stability at relatively high temperatures. (wikipedia.org)