Afipia
Cat-Scratch Disease
A self-limiting bacterial infection of the regional lymph nodes caused by AFIPIA felis, a gram-negative bacterium recently identified by the Centers for Disease Control and Prevention and by BARTONELLA HENSELAE. It usually arises one or more weeks following a feline scratch, with raised inflammatory nodules at the site of the scratch being the primary symptom.
Afipia felis induces uptake by macrophages directly into a nonendocytic compartment. (1/7)
Afipia felis is a Gram-negative bacterium that causes some cases of human Cat Scratch Disease. A. felis can survive and multiply in several mammalian cell types, including macrophages, but the precise intracellular compartmentalization of A. felis-containing phagosomes is unknown. Here, we demonstrate that, in murine macrophages, most A. felis-containing phagosomes exclude lysosomal tracer loaded into macrophage lysosomes before, as well as endocytic tracer loaded after, establishment of an infection. Established Afipia-containing phagosomes possess neither early endosomal marker proteins [early endosome antigen 1 (EEA1), Rab5, transferrin receptor, trytophane aspartate containing coat protein (TACO)] nor late endosomal or lysosomal proteins [cathepsin D, beta-glucuronidase, vacuolar proton-pumping ATPase, rab7, mannose-6-phosphate receptor, vesicle-associated membrane protein 8, lysosome-associated membrane proteins LAMP-1 and LAMP-2]. Those bacteria that will be found in a nonendosomal compartment enter the macrophage via an EEA1-negative compartment, which remains negative for LAMP-1. The smaller subpopulation of afipiae whose phagosomes will be part of the endocytic system enters into an EEA1-positive compartment, which also subsequently acquires LAMP-1. Killing of Afipia or opsonization with immune antibodies leads to a strong increase in the percentage of A. felis-containing phagosomes that interact with the endocytic system. We conclude that most phagosomes containing A. felis are disconnected from the endosome-lysosome continuum, that their unusual compartmentalization is decided at uptake, and that this compartmentalization requires bacterial viability. (+info)tfdA-like genes in 2,4-dichlorophenoxyacetic acid-degrading bacteria belonging to the Bradyrhizobium-Agromonas-Nitrobacter-Afipia cluster in alpha-Proteobacteria. (2/7)
The 2,4-dichlorophenoxyacetate (2,4-D)/alpha-ketoglutarate dioxygenase gene (tfdA) homolog designated tfdAalpha was cloned and characterized from 2,4-D-degrading bacterial strain RD5-C2. This Japanese upland soil isolate belongs to the Bradyrhizobium-Agromonas-Nitrobacter-Afipia cluster in the alpha subdivision of the class Proteobacteria on the basis of its 16S ribosomal DNA sequence. Sequence analysis showed 56 to 60% identity of tfdAalpha to representative tfdA genes. A MalE-TfdAalpha fusion protein expressed in Escherichia coli exhibited about 10 times greater activity for phenoxyacetate than 2,4-D in an alpha-ketoglutarate- and Fe(II)-dependent reaction. The deduced amino acid sequence of TfdAalpha revealed a conserved His-X-Asp-X(146)-His-X(14)-Arg motif characteristic of the active site of group II alpha-ketoglutarate-dependent dioxygenases. The tfdAalpha genes were also detected in 2,4-D-degrading alpha-Proteobacteria previously isolated from pristine environments in Hawaii and in Saskatchewan, Canada (Y. Kamagata, R. R. Fulthorpe, K. Tamura, H. Takami, L. J. Forney, and J. M. Tiedje, Appl. Environ. Microbiol. 63:2266-2272, 1997). These findings indicate that the tfdA genes in beta- and gamma-Proteobacteria and the tfdAalpha genes in alpha-Proteobacteria arose by divergent evolution from a common ancestor. (+info)Description of Afipia birgiae sp. nov. and Afipia massiliensis sp. nov. and recognition of Afipia felis genospecies A. (3/7)
On the basis of phenotypic characterization and DNA relatedness, two novel species are proposed, Afipia birgiae sp. nov. (type strain 34632T = CIP 106344T = CCUG 43108T) and Afipia massiliensis sp. nov. (type strain 34633T = CIP 107022T = CCUG 45153T). A new genospecies is described, named Afipia felis genospecies A, closely related to Afipia felis. The complexity encountered in the taxonomy of the Bradyrhizobiaceae group within the alpha-2 subgroup of the Proteobacteria is discussed and the description of these novel species highlights the need for new tools for phylogenetic analysis in the group. The novel species herein described are fastidious bacteria isolated from a hospital water supply in co-culture with amoebae. It is hypothesized that this group of bacteria are a potential cause of nosocomial infections. (+info)Usefulness of rpoB gene sequencing for identification of Afipia and Bosea species, including a strategy for choosing discriminative partial sequences. (4/7)
Bacteria belonging to the genera Afipia and Bosea are amoeba-resisting bacteria that have been recently reported to colonize hospital water supplies and are suspected of being responsible for intensive care unit-acquired pneumonia. Identification of these bacteria is now based on determination of the 16S ribosomal DNA sequence. However, the 16S rRNA gene is not polymorphic enough to ensure discrimination of species defined by DNA-DNA relatedness. The complete rpoB sequences of 20 strains were first determined by both PCR and genome walking methods. The percentage of homology between different species ranged from 83 to 97% and was in all cases lower than that observed with the 16S rRNA gene; this was true even for species that differed in only one position. The taxonomy of Bosea and Afipia is discussed in light of these results. For strain identification that does not require the complete rpoB sequence (4,113 to 4,137 bp), we propose a simple computerized method that allows determination of nucleotide positions of high variability in the sequence that are bordered by conserved sequences and that could be useful for design of universal primers. A fragment of 740 to 752 bp that contained the most highly variable area (positions 408 to 420) was amplified and sequenced with these universal primers for 47 strains. The variability of this sequence allowed identification of all strains and correlated well with results of DNA-DNA relatedness. In the future, this method could be also used for the determination of variability "hot spots" in sets of housekeeping genes, not only for identification purposes but also for increasing the discriminatory power of sequence typing techniques such as multilocus sequence typing. (+info)Rare bacterium of new genus isolated with prolonged enrichment culture. (5/7)
Dynamic change in microbial flora was monitored with an oxygen electrode. The 1st phase microorganisms, which first grew well in LB medium, were followed by the 2nd phase microorganisms, which supposedly assimilated microbial cells of the 1st phase and their metabolites. In a similar way, a change in microbial flora was observed from the 1st phase to the 4th phase in 84 hr. Based on this observation, prolonged enrichment culture was done for as long as two months to increase the ratio of existence of rare microorganisms. From these culture liquids, four slow-growing bacteria (provisionally named Shinshu-ah1, -ah2, -ah3, and -ah4), which formed scarcely visible small colonies, were isolated. Sequence analysis of their 16S rDNA showed that Shinshu-ah1 had 97% homology with Bradyrhizobium japonicum and uncultured alpha proteobacterium clone blaii 16, Shinshu-ah2 91% with Rasbo bacterium, Alpha proteobacterium 34619, Bradyrhizobium genosp. P, Afipia felis and an unidentified bacterium, Shinshu-ah3 99% with Methylobacterium mesophilicum, and Shinshu-ah4 95% with Agromyces ramosus DSM 43045. Phylogenetic study indicated that Shinshu-ah2 had a possibility to form a new family, Shinshu-ah1 a new genus, and Shinshu-ah4 a new species. (+info)Genome sequence of Afipia birgiae, a rare bacterium associated with Amoebae. (6/7)
(+info)Isolation of novel Afipia septicemium and identification of previously unknown bacteria Bradyrhizobium sp. OHSU_III from blood of patients with poorly defined illnesses. (7/7)
(+info)
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Afipia
... is a genus of bacteria in the Nitrobacteraceae family. The currently accepted taxonomy is based on the List of ... "Isolation of Novel Afipia septicemium and Identification of Previously Unknown Bacteria Bradyrhizobium sp. OHSU_III from Blood ...
Afipia clevelandensis
November 1991). "Proposal of Afipia gen. nov., with Afipia felis sp. nov. (formerly the cat scratch disease bacillus), Afipia ... Afipia clevelandensis is a species of the Afipia bacterial genus. It is a gram-negative, oxidase-positive, non-fermentative rod ... ISBN 3-8055-6649-2. LPSN "Afipia clevelandensis" at the Encyclopedia of Life Type strain of Afipia clevelandensis at BacDive - ... Drancourt M, Brouqui P, Raoult D (November 1997). "Afipia clevelandensis antibodies and cross-reactivity with Brucella spp. and ...
Afipia felis
Proposal of Afipia gen. nov., with Afipia felis sp. nov. (Formerly the Cat Scratch Disease Bacillus), Afipia clevelandensis sp ... Afipia felis is the type species of the Afipia bacterial genus. It was formerly thought to cause cat-scratch disease. It is a ... ISBN 3-8055-6649-2. LPSN "Afipia felis" at the Encyclopedia of Life Type strain of Afipia felis at BacDive - the Bacterial ... Birkness KA, George VG, White EH, Stephens DS, Quinn FD (June 1992). "Intracellular growth of Afipia felis, a putative ...
Afipia carboxidovorans
... is a Gram-negative soil bacterium. It is aerobic, the cells are rod-shaped. Hördt, Anton; López, Marina ...
Afipia birgiae
La Scola, B. (2002). "Description of Afipia birgiae sp. nov. and Afipia massiliensis sp. nov. and recognition of Afipia felis ... Afipia birgiae is a species in the Afipia bacterial genus. It is a gram-negative, oxidase-positive rod in the alpha-2 subgroup ... LPSN "Afipia birgiae" at the Encyclopedia of Life Type strain of Afipia birgiae at BacDive - the Bacterial Diversity ... and Afipia massiliensis sp. nov. and recognition of Afipia felis genospecies A". International Journal of Systematic and ...
Afipia broomeae
November 1991). "Proposal of Afipia gen. nov., with Afipia felis sp. nov. (formerly the cat scratch disease bacillus), Afipia ... Afipia broomeae is a species of the Afipia bacterial genus. It is a gram-negative, oxidase-positive, non-fermentative rod in ... ISBN 3-8055-6649-2. LPSN "Afipia broomeae" at the Encyclopedia of Life Type strain of Afipia broomeae at BacDive - the ... formerly the Cleveland Clinic Foundation strain), Afipia broomeae sp. nov., and three unnamed genospecies". Journal of Clinical ...
Afipia massiliensis
... is a species of the Afipia bacterial genus. It is a gram-negative, oxidase-positive rod in the alpha-2 ... and Afipia massiliensis sp. nov. and recognition of Afipia felis genospecies A". International Journal of Systematic and ... ISBN 3-8055-6649-2. LPSN "Afipia massiliensis" at the Encyclopedia of Life Type strain of Afipia massiliensis at BacDive - the ... Its type strain is 34633T (=CIP 107022T =CCUG 45153T). La Scola, B. (2002). "Description of Afipia birgiae sp. nov. ...
Nitrobacteraceae
This group [Afipia] is commonly found in aquatic environments and is known to use dimethyl sulfone (DMSO2) as a sole carbon ... The genus Afipia has also been found in the atmosphere, where it uses methylsulfonylmethane as a carbon source. The bacteria of ... It also contains animal-associated bacteria such as Afipia felis, formerly thought to cause cat-scratch disease. Others are ... Based on the taxonomical classification of the SSU rRNA gene sequences recovered, Afipia spp. (Alphaproteobacteria) comprised ...
Methylsulfonylmethane
This group [Afipia] is commonly found in aquatic environments and is known to use dimethyl sulfone (DMSO2) as a sole carbon ... where it is used as a carbon source by the airborne bacteria Afipia. Oxidation of dimethyl sulfoxide produces the sulfone, both ...
Mesorhizobium
"Isolation and Characterization of Two Novel Bacteria Afipia cberi and Mesorhizobium hominis from Blood of a Patient Afflicted ...
Bartonellosis
isolated and cultured a bacterium that was named Afipia felis in 1992 after the team at the Armed Forces Institute of Pathology ...
List of bacterial genera named after institutions
Afipia - AFIP (Armed Force Institute of Pathology), USA Basfia - BASF SE (a chemical company in Ludwigshafen, Germany) Cedecea ...
Cat-scratch disease
In 1983, the Warthin-Starry silver stain was used to discover a Gram-negative bacillus which was named Afipia felis in 1991 ... The causative organism of CSD was originally believed to be Afipia felis, but this was disproved by immunological studies in ...
Nitrobacter
... and the human pathogens Afipia felis and Afipia clevelandensis. Bacteria within the genus Nitrobacter are presumed to have ...
List of MeSH codes (B03)
Afipia MeSH B03.440.400.425.200.090 - Bradyrhizobium MeSH B03.440.400.425.200.520 - Nitrobacter MeSH B03.440.400.425.200.700 - ... Afipia MeSH B03.440.400.425.117 - alcaligenaceae MeSH B03.440.400.425.117.024 - Achromobacter MeSH B03.440.400.425.117.024.100 ... Afipia MeSH B03.660.050.035.090 - Bradyrhizobium MeSH B03.660.050.035.520 - Nitrobacter MeSH B03.660.050.035.700 - ...
Unraveling Mysteries Associated with Cat-Scratch Disease, Bacillary Angiomatosis, and Related Syndromes - Volume 1, Number 1...
Brenner DJ, Hollis DG, Moss CW, English CK, Proposal to Afipia gen. nov., with Afipia felis sp. nov. (Formerly the Cat Scratch ... By 1992, this agent was characterized fully, given the name Afipia felis (Afipia being a latinized acronym for the source of ... Enter Afipia felis Enter New Syndromes Enter Rochalimaea henselae The Cat-scratch Connection: A Synthesis Felis domesticus: A ... Enter Afipia felis. During the past 44 years, a variety of microbial agents, including herpes viruses and bacteria of the ...
Bacillary Angiomatosis Workup: Approach Considerations, Procedures, Laboratory Studies
DeCS - Termos Novos
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WikiGenes
Isolation and properties of methanesulfonate-degrading Afipia felis from Antarctica and comparison with other strains of A. ... Free-living Afipia strains occurring widely in the natural environment may be significant as methylotrophs, degrading C(1)- ... This is the first report of methanesulfonate-degrading bacteria from the Antarctic and of methylotrophy in Afipia, and the ... Isolation and properties of methanesulfonate-degrading Afipia felis from Antarctica and comparison with other strains of A. ...
Bacterial Skin Diseases
Unraveling Mysteries Associated with Cat-Scratch Disease, Bacillary Angiomatosis, and Related Syndromes - Volume 1, Number 1...
Brenner DJ, Hollis DG, Moss CW, English CK, Proposal to Afipia gen. nov., with Afipia felis sp. nov. (Formerly the Cat Scratch ... By 1992, this agent was characterized fully, given the name Afipia felis (Afipia being a latinized acronym for the source of ... Enter Afipia felis Enter New Syndromes Enter Rochalimaea henselae The Cat-scratch Connection: A Synthesis Felis domesticus: A ... Enter Afipia felis. During the past 44 years, a variety of microbial agents, including herpes viruses and bacteria of the ...
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Keywords
Fondation Recherches 3R / Projets / 3R-Project 99-05
Dynacin, Minocin (minocycline) dosing, indications, interactions, adverse effects, and more
DeCS - Términos Nuevos
DeCS
Afipia - Preferred Concept UI. M0328409. Scope note. A genus of gram-negative, oxidase-positive, nonfermentative rods which are ... Afipia felis Scope note:. Género de bacilos gramnegativos, oxidasa positivos, no fermentadores, móviles por un único flagelo. ... Afipia felis y BARTONELLA HENSELAE son agentes causales de la ENFERMEDAD DE ARAÑAZO DE GATO. (Bergeys Manual of Determinative ... Afipia felis and BARTONELLA HENSELAE are causative agents of CAT-SCRATCH DISEASE. (From Bergeys Manual of Determinative ...
Code System Concept
DeCS - Términos Nuevos
DeCS - Termos Novos
DeCS - Términos Nuevos
DeCS - New terms
DeCS - New terms
DeCS - Termos Novos
DeCS - Términos Nuevos
DeCS - New terms
DeCS - Termos Novos
DeCS - Términos Nuevos
DeCS - New terms
DeCS - New terms
DeCS - New terms
Felis1
- Afipia felis and BARTONELLA HENSELAE are causative agents of CAT-SCRATCH DISEASE. (bvsalud.org)
Species1
- Although the causative agent has not been isolated with certainty, it is believed to be a species of Rochalimaea or Afipia. (cliffsnotes.com)
Felis and Rochalimaea henselae2
- Dual role for Afipia felis and Rochalimaea henselae in cat-scratch disease. (nih.gov)
- The results of studies on the serologic responses to Afipia felis and Rochalimaea henselae in suspected patients for Cat Scratch Disease (CSD) are illustrated. (elsevier.com)
Bartonella1
- Afipia felis and BARTONELLA HENSELAE are causative agents of CAT-SCRATCH DISEASE . (nih.gov)
Bacteria2
- Other than in portable water and air, bacteria and fungi typically co-exist in [http://en.wikipedia.org/wiki/Biofilm biofilms] on surfaces, including water pipe lines. (kenyon.edu)
- Genome sequences for three strains of denitrifying bacteria (Alphaproteobacteria-Afipia sp. (uic.edu)
Strains1
- 2005). Isolation and properties of methanesulfonate-degrading Afipia felis from Antarctica and comparison with other strains of A. felis . (up.pt)