Affinity Labels: Analogs of those substrates or compounds which bind naturally at the active sites of proteins, enzymes, antibodies, steroids, or physiological receptors. These analogs form a stable covalent bond at the binding site, thereby acting as inhibitors of the proteins or steroids.DiazomethaneProlyl Hydroxylases: Enzymes that specifically hydroxylate PROLINE residues on proteins.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Kinetics: The rate dynamics in chemical or physical systems.Alkylation: The covalent bonding of an alkyl group to an organic compound. It can occur by a simple addition reaction or by substitution of another functional group.Binding, Competitive: The interaction of two or more substrates or ligands with the same binding site. The displacement of one by the other is used in quantitative and selective affinity measurements.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Chromatography, Affinity: A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Biotin: A water-soluble, enzyme co-factor present in minute amounts in every living cell. It occurs mainly bound to proteins or polypeptides and is abundant in liver, kidney, pancreas, yeast, and milk.Adenosine Diphosphate: Adenosine 5'-(trihydrogen diphosphate). An adenine nucleotide containing two phosphate groups esterified to the sugar moiety at the 5'-position.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Cyanogen Bromide: Cyanogen bromide (CNBr). A compound used in molecular biology to digest some proteins and as a coupling reagent for phosphoroamidate or pyrophosphate internucleotide bonds in DNA duplexes.Peptide Fragments: Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.Indicators and Reagents: Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)Glutamate Dehydrogenase: An enzyme that catalyzes the conversion of L-glutamate and water to 2-oxoglutarate and NH3 in the presence of NAD+. (From Enzyme Nomenclature, 1992) EC 1.4.1.2.Amino Acids: Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.Chromatography, High Pressure Liquid: Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.Molecular Structure: The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.Cysteine: A thiol-containing non-essential amino acid that is oxidized to form CYSTINE.Structure-Activity Relationship: The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.Lysine: An essential amino acid. It is often added to animal feed.Spin Labels: Molecules which contain an atom or a group of atoms exhibiting an unpaired electron spin that can be detected by electron spin resonance spectroscopy and can be bonded to another molecule. (McGraw-Hill Dictionary of Chemical and Technical Terms, 4th ed)Peptide Mapping: Analysis of PEPTIDES that are generated from the digestion or fragmentation of a protein or mixture of PROTEINS, by ELECTROPHORESIS; CHROMATOGRAPHY; or MASS SPECTROMETRY. The resulting peptide fingerprints are analyzed for a variety of purposes including the identification of the proteins in a sample, GENETIC POLYMORPHISMS, patterns of gene expression, and patterns diagnostic for diseases.Molecular Weight: The sum of the weight of all the atoms in a molecule.Adenosine: A nucleoside that is composed of ADENINE and D-RIBOSE. Adenosine or adenosine derivatives play many important biological roles in addition to being components of DNA and RNA. Adenosine itself is a neurotransmitter.NAD: A coenzyme composed of ribosylnicotinamide 5'-diphosphate coupled to adenosine 5'-phosphate by pyrophosphate linkage. It is found widely in nature and is involved in numerous enzymatic reactions in which it serves as an electron carrier by being alternately oxidized (NAD+) and reduced (NADH). (Dorland, 27th ed)Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Trypsin: A serine endopeptidase that is formed from TRYPSINOGEN in the pancreas. It is converted into its active form by ENTEROPEPTIDASE in the small intestine. It catalyzes hydrolysis of the carboxyl group of either arginine or lysine. EC 3.4.21.4.Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Antibody Affinity: A measure of the binding strength between antibody and a simple hapten or antigen determinant. It depends on the closeness of stereochemical fit between antibody combining sites and antigen determinants, on the size of the area of contact between them, and on the distribution of charged and hydrophobic groups. It includes the concept of "avidity," which refers to the strength of the antigen-antibody bond after formation of reversible complexes.Hydrogen-Ion Concentration: The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Liver: A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Receptor-mediated targeting of fluorescent probes in living cells. (1/2197)

A strategy was developed to label specified sites in living cells with a wide selection of fluorescent or other probes and applied to study pH regulation in Golgi. cDNA transfection was used to target a single-chain antibody to a specified site such as an organelle lumen. The targeted antibody functioned as a high affinity receptor to trap cell-permeable hapten-fluorophore conjugates. Synthesized conjugates of a hapten (4-ethoxymethylene-2-phenyl-2-oxazolin-5-one, phOx) and fluorescent probes (Bodipy Fl, tetramethylrhodamine, fluorescein) were bound with high affinity (approximately 5 nM) and specific localization to the single-chain antibody expressed in the endoplasmic reticulum, Golgi, and plasma membrane of living Chinese hamster ovary cells. Using the pH-sensitive phOx-fluorescein conjugate and ratio imaging microscopy, pH was measured in the lumen of Golgi (pH 6.25 +/- 0.06). Measurements of pH-dependent vacuolar H+/ATPase pump activity and H+ leak in Golgi provided direct evidence that resting Golgi pH is determined by balanced leak-pump kinetics rather than the inability of the H+/ATPase to pump against an electrochemical gradient. Like expression of the green fluorescent protein, the receptor-mediated fluorophore targeting approach permits specific intracellular fluorescence labeling. A significant advantage of the new approach is the ability to target chemical probes with custom-designed spectral and indicator properties.  (+info)

Interaction of purified human proteinase 3 (PR3) with reconstituted lipid bilayers. (2/2197)

Proteinase 3 (PR3), the major target autoantigen in Wegener's granulomatosis is a serine proteinase that is normally stored intracellularly in the primary granules of quiescent neutrophils and monocytes. Upon cell activation, a significant portion of this antigen is detected on the cell surface membrane. The nature of the association of PR3 with the membrane and its functional significance are unknown. We investigated the interaction of purified human PR3 with mixtures of zwitterionic (dimyristoyl-L-alpha-phosphatidylcholine, DMPC) and anionic (dimyristoyl-L-alpha-phosphatidylglycerol, DMPG) phospholipids in reconstituted lipid bilayers using differential scanning calorimetry and lipid photolabeling, and measured the affinity of this interaction using spectrophotometry. Two other primary granule constituents, human neutrophil elastase (HNE) and myeloperoxidase (MPO) were investigated for comparison. In calorimetric assays, using lipid vesicles of mixed DMPC/DMPG, increasing PR3 concentrations (protein/lipid molar ratio from 0 to 1 : 110) induced a significant decrease of the main chain transition enthalpy and a shift in chain melting temperatures which is indicative of partial insertion of PR3 into the hydrophobic region of the lipid membranes. This was confirmed by hydrophobic photolabeling using liposomes containing trace amounts of the photoactivable [125I]-labeled phosphatidylcholine analog TID-PC/16. The molar affinity of PR3, HNE, and MPO to lipid vesicles of different DMPC/DMPG ratios was then determined by spectrophotometry. At a DMPC/DMPG ratio of 1 : 1, molar affinities of PR3, Kd = 4.5 +/- 0.3 microm; HNE, 14.5 +/- 1.2 microm; and MPO, 50 +/- 5 microm (n = 3) were estimated. The lipid-associated PR3 exhibited two-fold lower Vmax and Km values, and its enzyme activity was slightly more inhibited (Ki) by the natural alpha1-proteinase inhibitor (alpha1-PI) or an autoantibody to PR3.  (+info)

Affinity labelling with MgATP analogues reveals coexisting Na+ and K+ forms of the alpha-subunits of Na+/K+-ATPase. (3/2197)

To test the hypothesis that Na+/K+-ATPase works as an (alpha beta)2-diprotomer with interacting catalytic alpha-subunits, tryptic digestion of pig kidney enzyme, that had been inactivated with substitution-inert MgATP complex analogues, was performed. This led to the demonstration of coexisting C-terminal Na+-like 80-kDa as well as K+-like 60-kDa peptides and N-terminal 40-kDa peptides of the alpha-subunit. To localize the ATP binding sites on tryptic peptides, studies with radioactive MgATP complex analogues were performed: Co(NH3)4-8-N3-ATP specifically modified the E2ATP (low affinity) binding site of Na+/K+-ATPase with an inactivation rate constant (k2) of 12 x 10-3.min-1 at 37 degrees C and a dissociation constant (Kd) of 207 +/- 28 microm. Tryptic digestion of the [gamma32P]Co(NH3)4-8-N3-ATP-inactivated and photolabelled alpha-subunit (Mr = 100 kDa) led, in the absence of univalent cations, to a K+-like C-terminal 60-kDa fragment which was labelled in addition to an unlabelled Na+-like C-terminal 80-kDa fragment. Tryptic digestion of [alpha32P]-or [gamma32P]Cr(H2O)4ATP - bound to the E1ATP (high affinity) site - led to the labelling of a Na+-like 80-kDa fragment besides the immediate formation of an unlabelled K+-like N-terminal 40-kDa fragment and a C-terminal 60-kDa fragment. Because a labelled Na+-like 80-kDa fragment cannot result from an unlabelled K+-like 60-kDa fragment, and because unlabelled alpha-subunits did not show any catalytic activity, the findings are consistent with a situation in which Na+- and K+-like conformations are stabilized by tight binding of substitution-inert MgATP complex analogues to the E1ATP and E2ATP sites. Hence, all data are consistent with the hypothesis that ATP binding induces coexisting Na+ and K+ conformations within an (alphabeta)2-diprotomeric Na+/K+-ATPase.  (+info)

Identification, purification, and characterization of the rat liver golgi membrane ATP transporter. (4/2197)

Phosphorylation of secretory and integral membrane proteins and of proteoglycans also occurs in the lumen of the Golgi apparatus. ATP, the phosphate donor in these reactions, must first cross the Golgi membrane before it can serve as substrate. The existence of a specific ATP transporter in the Golgi membrane has been previously demonstrated in vitro using intact Golgi membrane vesicles from rat liver and mammary gland. We have now identified and purified the rat liver Golgi membrane ATP transporter. The transporter was purified to apparent homogeneity by a combination of conventional ion exchange, dye color, and affinity chromatography. An approximately 70,000-fold purification (2% yield) was achieved starting from crude rat liver Golgi membranes. A protein with an apparent molecular mass of 60 kDa was identified as the putative transporter by a combination of column chromatography, photoaffinity labeling with an analog of ATP, and native functional size determination on a glycerol gradient. The purified transporter appears to exist as a homodimer within the Golgi membrane, and when reconstituted into phosphatidylcholine liposomes, was active in ATP but not nucleotide sugar or adenosine 3'-phosphate 5'-phosphosulfate transport. The transport activity was saturable with an apparent Km very similar to that of intact Golgi vesicles.  (+info)

Ectodomain cleavage and shedding of the type III transforming growth factor-beta receptor in lung membranes effect of temperature, ligand binding and membrane solubilization. (5/2197)

Previous studies from our laboratory [Philip, A. & O'Connor-McCourt, M. D. (1991) J. Biol. Chem. 266, 22290--22296] have shown that the lung exhibited the highest uptake of circulating [125I]-transforming growth factor-beta1 (TGF-beta1) on a per gram basis. This observation, together with the lack of information on TGF-beta receptor expression in the lung, prompted us to attempt to characterize TGF-beta receptors in this tissue. In the present report we show that the type III TGF-beta receptor is the most abundant TGF-beta binding protein in rat lung membranes and that it exhibits a 10-fold higher affinity for TGF-beta2 than for TGF-beta1. We observed that the majority of the type III receptor population in lung membranes is cleaved at a site in the central portion of the ectodomain, the resulting two fragments (95 kDa and 58 kDa) being held together by disulfide bonds. Furthermore, we demonstrate that a soluble form of the ectodomain of the type III receptor is shed from rat lung membranes in an efficient manner, with protease cleavage occurring at a site close to the transmembrane domain. This shedding is controllable by temperature, thus providing a system to study the mechanism of ectodomain release. Using this system, we show that the shedding is inhibited by prior ligand binding and by membrane solubilization. The identification of a membrane preparation which exhibits controllable and quantitative release of the type III receptor ectodomain provides a unique cell-free system for further studies of the mechanism of shedding of the type III TGF-beta receptor ectodomain.  (+info)

Photoaffinity labeling and mass spectrometry identify ribosomal protein S3 as a potential target for hybrid polar cytodifferentiation agents. (6/2197)

The ability of a novel class of hybrid polar compounds (HPCs) to induce differentiation and consequent cessation of proliferation of transformed cells has led to their development as potential chemotherapeutic agents in the treatment of cancer. Suberoylanilide hydroxamic acid (SAHA) is a prototype of a family of hydroxamic acid based compounds (SAHA-like HPCs) that can, at micromolar concentrations, induce a variety of transformed cell lines to differentiate. The mechanism of action of the HPCs is not entirely understood. Searching for a cellular target of the SAHA-like HPCs, we synthesized a photoaffinity labeling reagent structurally based on SAHA, and probed for SAHA-binding proteins in murine erythroleukemia (MEL) cells. Photoaffinity labeling in cell free extracts identified a 32-kDa protein (p32) that was specifically labeled by the photoaffinity reagent. Cell fractionation assays localized p32 to the P100 fraction. p32 was partially purified and identified by mass spectrometry as the 40 S ribosomal protein S3. Expression of epitope-tagged S3 in bacterial lysates followed by photoaffinity labeling confirmed its specific labeling. Identification of a cytodifferentiation agent target may shed light on the mechanism by which the SAHA-like HPCs exert their antitumor effects.  (+info)

Comparison of paclitaxel-, 5-fluoro-2'-deoxyuridine-, and epidermal growth factor (EGF)-induced apoptosis. Evidence for EGF-induced anoikis. (7/2197)

Epidermal growth factor (EGF), a hormone that stimulates proliferation of many cell types, induces apoptosis in some cell lines that overexpress the EGF receptor. To evaluate the mechanism of EGF-induced apoptosis, MDA-MB-468 breast cancer cells were examined by microscopy, flow cytometry, immunoblotting, enzyme assays, and affinity labeling after treatment with EGF, paclitaxel, or 5-fluoro-2'-deoxyuridine (5FUdR). Apoptosis induced by all three agents was accompanied by activation of caspases-3, -6, and -7, as indicated by disappearance of the corresponding zymogens from immunoblots, cleavage of substrate polypeptides in situ, and detection of active forms of these caspases in cytosol and nuclei using fluorogenic assays and affinity labeling. Further analysis indicated involvement of the cytochrome c/Apaf-1/caspase-9 pathway of caspase activation, but not the Fas/Fas ligand pathway. Interestingly, caspase activation was consistently lower after EGF treatment than after paclitaxel or 5FUdR treatment. Additional experiments revealed that the majority of cells detaching from the substratum after EGF (but not paclitaxel or 5FUdR) were morphologically normal and retained the capacity to readhere, suggesting that EGF-induced apoptosis involves cell detachment followed by anoikis. These observations not only indicate that EGF- and chemotherapy-induced apoptosis in this cell line involve the same downstream pathways but also suggest that detachment-induced apoptosis is responsible for the paradoxical antiproliferative effects of EGF.  (+info)

Opening mechanism of a cyclic nucleotide-gated channel based on analysis of single channels locked in each liganded state. (8/2197)

Cyclic nucleotide-gated channels contain four subunits, each with a binding site for cGMP or cAMP in the cytoplasmic COOH-terminal domain. Previous studies of the kinetic mechanism of activation have been hampered by the complication that ligands are continuously binding and unbinding at each of these sites. Thus, even at the single channel level, it has been difficult to distinguish changes in behavior that arise from a channel with a fixed number of ligands bound from those that occur upon the binding and unbinding of ligands. For example, it is often assumed that complex behaviors like multiple conductance levels and bursting occur only as a consequence of changes in the number of bound ligands. We have overcome these ambiguities by covalently tethering one ligand at a time to single rod cyclic nucleotide-gated channels (Ruiz, ML., and J.W. Karpen. 1997. Nature. 389:389-392). We find that with a fixed number of ligands locked in place the channel freely moves between three conductance states and undergoes bursting behavior. Furthermore, a thorough kinetic analysis of channels locked in doubly, triply, and fully liganded states reveals more than one kinetically distinguishable state at each conductance level. Thus, even when the channel contains a fixed number of bound ligands, it can assume at least nine distinct states. Such complex behavior is inconsistent with simple concerted or sequential allosteric models. The data at each level of liganding can be successfully described by the same connected state model (with different rate constants), suggesting that the channel undergoes the same set of conformational changes regardless of the number of bound ligands. A general allosteric model, which postulates one conformational change per subunit in both the absence and presence of ligand, comes close to providing enough kinetically distinct states. We propose an extension of this model, in which more than one conformational change per subunit can occur during the process of channel activation.  (+info)

*Affinity label

Affinity labels are molecules similar in structure to a particular substrate for a specific enzyme and are considered to be a ... The label binds covalently to the enzyme so that the substrate can no longer bind, causing a permanent and irreversible change ...

*David Rasnick

... received a Ph.D. in chemistry from Georgia Tech in 1978; his thesis was entitled "Affinity Labeling of ... Rasnick, David William (1978). "Affinity labeling of metalloendoproteases" (electronic thesis or dissertation). Retrieved 28 ...

*Quiet Now

It was released in 1981 on the Affinity label. In 1999, Polygram issued a compilation titled Quiet Now: Never Let Me Go which, ...

*Phosphoribosylglycinamide formyltransferase

Inglese, J.; Johnson, D.L.; Shiau, A.; Smith, J.M.; Benkovic, S.J. (1990). "Subcloning, Characterization, and Affinity Labeling ... Using a bromoacetyl dideazafolate affinity analog James Inglese and colleagues first identified Asp144 as an active site ...

*Acrylfentanyl

Maryanoff, Bruce E.; Simon, Eric J.; Gioannini, Theresa; Gorissen, H. (August 1982). "Potential affinity labels for the opiate ... Essawi, M. Y. (April 1999). "Fentanyl analogues with a modified propanamido group as potential affinity labels: synthesis and ... "Potential affinity labels for the opiate receptor based on fentanyl and related compounds". Journal of Medicinal Chemistry. 25 ...

*Ribosome

Affinity label for the tRNA binding sites on the E. coli ribosome allowed the identification of A and P site proteins most ... During 1977, Czernilofsky published research that used affinity labeling to identify tRNA-binding sites on rat liver ribosomes ... "SITE OF REACTION ON RIBOSOMAL-PROTEIN L27 WITH AN AFFINITY LABEL DERIVATIVE OF TRANSFER-RNA-F(MET)". FEBS Letters. ELSEVIER ... "IDENTIFICATION OF TRNA-BINDING SITES ON RAT-LIVER RIBOSOMES BY AFFINITY LABELING". Molecular and General Genetics. Springer ...

*Outer membrane phospholipase A1

"Inactivation of Escherichia coli outer-membrane phospholipase a by the affinity label hexadecanesulfonyl fluoride. Evidence for ...

*40S ribosomal protein S5

Affinity labelling of the 40S subunits by derivatives of oligoribonucleotides containing the AUG codon". Biochim. Biophys. Acta ...

*RPS17

Affinity labelling of the 40S subunits by derivatives of oligoribonucleotides containing the AUG codon". Biochim. Biophys. Acta ...

*Methods to investigate protein-protein interactions

Mamunul; Lee, Jun-Seok (2017-06-24). "Photo-affinity labeling (PAL) in chemical proteomics: a handy tool to investigate protein ... In a label transfer reaction, a known protein is tagged with a detectable label. The label is then passed to an interacting ... Affinity electrophoresis as used for estimation of binding constants, as for instance in lectin affinity electrophoresis or ... Label transfer can also detect weak or transient interactions that are difficult to capture using other in vitro detection ...

*Glutathione S-transferase

Vargo MA, Colman RF (January 2001). "Affinity labeling of rat glutathione S-transferase isozyme 1-1 by 17beta -iodoacetoxy- ... Because the GST protein has a strong binding affinity for GSH, beads coated with the compound can be added to the protein ... Affinity chromatography Bacterial glutathione transferase Glutathione S-transferase Mu 1 Glutathione S-transferase, C-terminal ... Benard V, Bokoch GM (2002). "Assay of Cdc42, Rac, and Rho GTPase activation by affinity methods". Meth. Enzymol. 345: 349-59. ...

*Glycoside hydrolase family 14

Sakiyama F, Nitta Y, Isoda Y, Toda H (1989). "Identification of glutamic acid 186 affinity-labeled by 2,3-epoxypropyl alpha-D- ...

*AKR1B1

Affinity labeling of an active site lysine by pyridoxal 5'-phosphate and pyridoxal 5'-diphospho-5'-adenosine". The Journal of ...

*Meir Wilchek

... for affinity chromatography, affinity labeling, affinity therapy, and the avidin-biotin system. The Avidin-biotin complex is ... Specific uses of affinity chromatography include antibody affinity, Immobilized metal ion affinity chromatography and ... Strausbauch, P.H., Weinstein, Y., Wilchek, M., Shaltiel, S., Givol, D. (1971). "A homologous series of affinity labeling ... thus laying the grounds for modern hemoperfusion Affinity label is a molecule that is similar in structure to a particular ...

*2-hydroxymuconate tautomerase

... affinity labeling and heteronuclear NMR studies". Biochemistry. 35 (3): 803-813. doi:10.1021/bi951077g. PMID 8547260. Wang, S.C ...

*Lanosterol synthase

Affinity Labeling with Carbocations Derived from Mechanism-Based Analogs of 2, 3-Oxidosqualene and Site-Directed Mutagenesis ... Since the enzyme affinity for this second substrate is greater than for the monoepoxy (S)-2,3-epoxysqualene, under partial ...

*Corticosteroid 11-beta-dehydrogenase isozyme 2

1996). "Purification of 11 beta-hydroxysteroid dehydrogenase type 2 from human placenta utilizing a novel affinity labelling ... and binds with equal affinity to the mineralocorticoid receptor, thereby out-competing aldosterone in cells that do not produce ...

*Squalene-hopene cyclase

Zheng, Y.; Abe, I.; Prestwich, G. (1998). "Inhibition kinetics and affinity labeling of bacterial squalene:hopene cyclase by ...

*Transfer RNA

The P-site protein L27 has been determined by affinity labeling by E. Collatz and A.P. Czernilofsky (FEBS Lett., Vol. 63, pp ... sites have been determined by affinity labeling by A.P. Czernilofsky et al. (Proc. Natl. Acad. Sci, USA, pp 230-234, 1974). ...

*HSD17B1

Identification of a single histidine residue affinity-labeled by both 3-bromoacetoxyestrone and 12 beta-bromoacetoxy-4-estrene- ...

*Protein-ribulosamine 3-kinase

"Mapping of the ATP-binding domain of human fructosamine 3-kinase-related protein by affinity labelling with 5′-[p-( ...

*Diazirine

3H-diazirin-3-yl functional group for photo-affinity labeling". Bioorganic & Medicinal Chemistry. 17 (15): 5388-5395. doi: ... Yet, as this feature minimizes unspecific labeling, it is actually an advantage of using diazirines. Diazirines are often used ... Diazirines are widely used in receptor labeling studies. This is because diazirine-containing analogs of various ligands can be ... They are often used in photoaffinity labeling studies to observe a variety of interactions, including ligand-receptor, ligand- ...

*John Katzenellenbogen

Katzenellenbogen developed the first affinity label for the estrogen receptor that was widely used to characterize its physical ... "Identification of cysteine 530 as the covalent attachment site of an affinity-labeling estrogen (ketononestrol aziridine) and ... Liu, A.; Carlson, K. E.; Katzenellenbogen, J. A. (1992-05-29). "Synthesis of high affinity fluorine-substituted ligands for the ... 18-labeled estrogens and their selective uptakes in target tissues of immature rats". Journal of Nuclear Medicine: Official ...

*Fatty acid synthase

Joshi AK, Rangan VS, Smith S (February 1998). "Differential affinity labeling of the two subunits of the homodimeric animal ...

*HSD3B1

... change that mediates the sequential 3 beta-hydroxysteroid dehydrogenase/isomerase activities is supported by affinity labeling ...

*Chilean art

The group is characterised by their affinity with Impressionism and Fauvism, and their command of drawing and harmonious ... its importance for Chilean art become noticeable in the generation labelled by Antonio Romera as the "Generación del medio ...
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Find quality suppliers and manufacturers of 1717-59-5(2-(Fluorosulfonyl)difluoroacetic acid) for price inquiry. where to buy 1717-59-5(2-(Fluorosulfonyl)difluoroacetic acid).Also offer free database of 1717-59-5(2-(Fluorosulfonyl)difluoroacetic acid) including MSDS sheet(poisoning, toxicity, hazards and safety),chemical properties,Formula, density and structure, solution etc.
Development of photoaffinity analogs has provided a powerful tool for the investigation of different aspects of enzyme-substrate or enzyme-inhibitor interactions. Since UGTs are closely related membrane-bound isoenzymes, photoaffinity experiments previously published were performed on microsomal membrane fractions containing the entire range of UGT isoforms with overlapping substrate specificity (3, 4, 7, 10, 15). Development of specific UGT cDNAs and their expression in various cellular systems allows characterization to be carried out with individual UGTs in isolation from other isoforms.. The work presented here demonstrates that [β32P]5N3UDP-GlcUA is a specific photoaffinity probe for recombinant human liver UGT1*6. Photoaffinity labeling followed the criteria established by Haley for active site photolabeling (17): First, proteins which specifically bind UDP-GlcUA are photolabeled. As shown in fig. 4, two polypeptides in the molecular weight range of UGTs are photolabeled in membrane ...
You are viewing an interactive 3D depiction of the molecule 2-({6-[(bromoacetyl)amino]hexanoyl}amino)-2-deoxy-d-glucose (C14H25BrN2O7) from the PQR.
Use Absolute Mag™ Bromoacetyl Magnetic Particles, Silica, 1.0 μm for fast, easy, and consistent Proteins/Ligands Immobilization
Sulfonated 2-acryloyltaxol and sulfonated 2-O-acyl acid taxol derivatives are synthesized which have improved water solubility and stability while maintaining bio-activity. In particular, 2-[(3-sulfo-1-oxopropyl)oxy]taxol sodium salt is synthesized by reacting taxol with acrylic acid, and subsequently reacting the 2-acryloyltaxol with bisulfite in a Michael reaction. 2-{[4-((2-sulfoethyl)amino)-1,4-dioxobutyl]oxy}taxol sodium salt and 2-{[4-((3-sulfopropyl)amino-1,4-dioxobutyl]oxy}taxol sodium salt are synthesized by reacting 2-succinyltaxol with the tetrabutylammonium salts of taurine and 3-aminopropyl sulfonic acid, respectively, and subsequently exchanging the ammonium with sodium. Glycol derivatives of 2-O-acyl acid taxols with improved water solubility are synthesized by reaction of a glycol with 2-O-acyl acid taxol.
A novel process is disclosed for preparing (ω-fluorosulfonyl)haloaliphatic carboxylic acid fluorides by electrolytic fluorination, simply and efficiently.
Tracing cell division using a fluorescent label allows scientists to detect and quantify cell proliferation through multiple generations (Figure 1). Our Cell-ID™ Cell Proliferation Kits (Table 1) contain specially designed fluorescent labels, which can easily cross the plasma membrane and covalently label with protein inside cells without affecting morphology or physiology. During cell division, the fluorescent label is divided evenly between two daughter cells. Based on the fluorescence intensity, the proliferating cells can be easily detected up to 6 to 10 generations, even after several days in a cell culture environment or following fixation (Figure 2 ...
87075-47-6 - VPYLECDBJZNETQ-HLGREYGBSA-N - 2,3-Dialdehyde NADP - Similar structures search, synonyms, formulas, resource links, and other chemical information.
Our PARP1-Trap is a high quality PARP1-binding protein coupled to a monovalent matrix (agarose particles) for biochemical analysis of PARP1 and interacting partners.
Type II iodothyronine 5-deiodinase (5D-II) catalyzes the intracellular conversion of thyroxine (T4) to 3,5,3-triiodothyronine (T3), producing greater than 90% of the bioactive thyroid hormone in the cerebral cortex. In cultured glial cells, expression of this enzyme is cAMP dependent. Exploiting the cAMP-dependent nature of this enzyme in these cells and utilizing N-bromoacetyl-L-3- or 5-[125I]thyroxine (BrAc[125I]T4) to affinity label cellular proteins, a 27-kDa protein with the properties of this enzyme was identified. Intact cells labeled with BrAc[125I]T4 showed three prominent radiolabeled bands of proteins of Mr 55,000, 27,000, and 18,000 (p55, p27, p18, respectively) which incorporated approximately 80% of the affinity label. All three affinity-labeled proteins were membrane associated. One protein (p27) increased 5-6-fold after treating the cells for 16 h with dibutyryl cAMP; maximal specific incorporation of affinity label into the stimulated p27 was approximately 2 pmol/mg of cell protein
3-[(4-Ethoxy-1,4-dioxobutyl)amino]-2-pyridinecarboxylic acid ethyl ester/ACM676596615 can be provided in Alfa Chemistry. We are dedicated to provide our customers the best products and services.
Is specificity an important issue regarding affinity reagents? What about the validation of affinity reagents today, is it good enough? This depends on the application and the producer of the reagent. Validation should be the most important marketing argument that can be found.Today there is a continuous growth of both the number of affinity reagents that are produced and the different types of affinity reagents that are developed. In proteomics they become more and more important in exploring the human proteome. Therefore, validated affinity reagents should be on top of every proteomic researchers list. How should this be accomplished?Better international agreements on how affinity reagents should be tested to be regarded as functional reagents are needed. One of the most important issues is the specificity of the affinity reagent. An international standard for which specific validation that is needed for different kinds of applications would be very useful.In this thesis, it is shown that the ...
Smolinsky, G. and Pryde, G. A. (1971) The chemistry of vinyl azides, in The Azido Group (1971) (ed S. Patai), John Wiley & Sons, Ltd., Chichester, UK. doi: 10.1002/9780470771266.ch10 ...
Synonyms C.I. 77947; C.I. Pigment White 4; Zinc oxide [USAN]; zincoxideheavy; flowers of zinc; zinc white; zinc oxide,edible; active zinc oxide; zinkoxyd aktiv; zinci oxidum; activox; activox b; actox14; zine oxide; zine white; zincoxide; actox16; actox216; ai3-00277; akro-zincbar85; akro-zincbar90; amalox; azo22; azo-33; azo-55; azo-55tt; azo-66; azo-66tt; ZNO; oxozinc; zinc oxygen(-2) anion ...
The Myc-Trap is a single chain polypeptide antibody coupled to agarose beads for immunoprecipitation / pull down of Myc-tagged proteins.
3-Azido-3-deoxy-myo-inositol (3-AMI) is a D-3-deoxy-3-substituted myo-inositol derivative and intracellular phosphatidinylinositol signal inhibitor. It was
In this article, we are discussing the description "Thermal labels" that often leads to many queries when raised by a customer buying the product. With the purpose of avoiding mistakes in ordering and customer returns when buying these thermal labels, here is the information about common types of thermal labels:- Direct Thermal Coated Labels These ...
A number of reactive dichlorotriazine dyes specifically and irreversibly inactivate pig heart lactate dehydrogenase, yeast glucose 6-phosphate dehydrogenase and yeast hexokinase at sites competitive with NAD+, NADP+, and ATP respectively. Monochlorotriazine dyes, including Cibacron Blue F3G-A, do not inactivate lactate dehydrogenase but display high affinity and thus inhibit the inactivation by dichlorotriazine dyes. These data are interpreted in terms of the ability of nucleotide-binding enzymes to bind polysulphonated aromatic chromophores.. ...
To investigate the role of the coreceptor CD8 and lipid rafts in cytotoxic T lymphocyte (CTL) activation, we used soluble mono-and multimeric H-2K,sup,d,/sup,-peptide complexes and cloned S14 CTL specific for a photoreactive derivative of the Plasmodium berghei circumsporozoite (PbCS) peptide 252-260 [PbCS(ABA)]. We report that activation of CTL in suspension requires multimeric K,sup,d,/sup,-PbCS(ABA) complexes co-engaging TCR and CD8. Using TCR ligand photo-cross-linking, we find that monomeric K,sup,d,/sup,-PbCS(ABA) complexes promote association of TCR/CD3 with CD8/p56,sup,lck,/sup,. Dimerization of these adducts results in activation of p56lck in lipid rafts, where phosphatases are excluded. Additional cross-linking further increases p56,sup,lck,/sup, kinase activity, induces translocation of TCR/CD3 and other signaling molecules to lipid rafts and intracellular calcium mobilization. These events are prevented by blocking Src kinases or CD8 binding to TCR-associated K,sup,d,/sup, molecules, ...
Azido azetikoa konposatu organiko bat da. Formula kimiko hau du: CH3-COOH (C2H4O2).. Kolorerik gabeko likidoa da. Azido azetikoa ozpinaren osagai nagusia da. Azido ahultzat sailkatzen den arren, kontzentrazio altuetan korrosiboa da, eta gizakiaren larruazalari kalte egin diezaioke.. Azido azetiko sinpleena azido karboxilikoa da. Konposatu garrantzitsua da erreaktiboetan eta industria kimikoan, batez ere zeluloide azetatoa, argazki filma eta polibinil azetatoa ekoizteko. Zuntz sintetikoetan ere erabil daiteke. Etxebizitzetan, diluituta erabiltzen da. Kaltzioa kentzeko (E260) azidotasun erregulatzailetzat eta ongailutzat erabiltzen da.. ...
Photoaffinity cross‐linking is a rapidly developing technology for studying biomolecular interactions, including protein ligand‐receptor binding
Photoaffinity cross‐linking is a rapidly developing technology for studying biomolecular interactions, including protein ligand‐receptor binding
Azido maliko (HO2CCH2CHOHCO2H) landare eta fruitu ugaritan -sagar berdeetan, batik bat- izaten den bi azidodun alkohola da. Landareen eta animalien zelulen metabolismoan parte hartzen du. 100 °C-tan urtzen diren kristalak eratzen ditu, eta uretan eta alkoholean urtzen da. Sintesiz ere lor daiteke, azido maleikoa berotuz, edo azido tartarikotik, sukzinikotik eta aspartikotik.. ...
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Read the Label Article - When purchasing grains, it is important to read and understand the label to ensure that the products you purchase are what were intended and are of the best possible quality.
Labels dont define but can be used to provide an illustrative and explanative outline. A label, like an idea, can be changed, altered, morphed, redefined. A brand cannot. I have been labelled,...
The report generally describes 5-bromoacetyl salicylamide, examines its uses, production methods, patents. 5-Bromoacetyl salicylamide market situation
Uridine-5-diphospho-α-D-galactose disodium salt (CAS 137868-52-1) Market Research Report 2018 aims at providing comprehensive data on uridine-5-diphospho-α-d-galactose
Excellent cycling performance of an electrode composed of silicon alone was achieved in a bis(fluorosulfonyl)amide (FSA)‐based electrolyte, with a high discharge capacity of 950 mA h g−1 observed even at the 500th cycle. To elucidate the reaction behavior of the Si electrode in an FSA‐based ionic liquid electrolyte, we investigated the change in the cross‐sectional morphology of the Si‐active material layer, the distribution of Li in the layer, and the crystallinity of Si on the electrode surface. By cross‐sectional scanning electron microscopy, we confirmed that the electrode thickness increased with the cycle number. The increase in thickness was less noticeable in the FSA‐based electrolyte than in an organic electrolyte. An elemental analysis of the electrode material revealed that a film derived from the electrolyte was formed not only on the surface but also inside of the electrode. Soft X‐ray emission spectroscopy demonstrated that the distribution of Li in the FSA‐based ...
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Wollscheid B., Bausch-Fluck D., Henderson C., OBrien R., Bibel M., Schiess R., Aebersold R., Watts J.D.. Although the classification of cell types often relies on the identification of cell surface proteins as differentiation markers, flow cytometry requires suitable antibodies and currently permits detection of only up to a dozen differentiation markers in a single measurement. We use multiplexed mass-spectrometric identification of several hundred N-linked glycosylation sites specifically from cell surface-exposed glycoproteins to phenotype cells without antibodies in an unbiased fashion and without a priori knowledge. We apply our cell surface-capturing (CSC) technology, which covalently labels extracellular glycan moieties on live cells, to the detection and relative quantitative comparison of the cell surface N-glycoproteomes of T and B cells, as well as to monitor changes in the abundance of cell surface N-glycoprotein markers during T-cell activation and the controlled differentiation of ...
5. Click Next to open the Create a Service , Hosted Service page. Type a valid third-level DNS name in the Service Name text box and click Check Availability. When moving a service, the name is the same as that for the original version. (You might need to wait a few minutes for the service deletion to propagate through the data center.). 6. Youll probably want all (or at least most) of your Hosted Service and Storage Accounts to share the same affinity group, so select the Yes, This Service Is Related to Some of My Other Hosted Service or Storage Accounts option button.. 7. If this is the first service or account in the Affinity Group, click the Create a New Affinity Group radio button, type a name for the new Affinity Group in the text box (USA-SouthCentral for this example), and select a data center location in the Region list:. ...
Boc Sciences offers cas 168567-90-6 2,3,4,6-Tetra-O-acetyl-1-azido-1-deoxy-a-D-galactopyranosyl cyanide in bulk,please inquire us to get a quote for 168567-90-6 2,3,4,6-Tetra-O-acetyl-1-azido-1-deoxy-a-D-galactopyranosyl cyanide.
The IUPHAR/BPS Guide to Pharmacology. [125I]7-azido-8-iodoketanserine ligand page. Quantitative data and detailed annnotation of the targets of licensed and experimental drugs.
Updated on 10 April 2010, 11.09pm We are having our next round of Burberry Blue Label preorders~ To get an inspiration of what bags to get, you can check out https://burberrybluelabel.wordpress.com. We are more likely to get items from this years collection but if youd like to order mid-2009 designs, well take in your order…
Accepted options are: - label: label to associate with the element - label_attributes: attributes to use when the label is rendered - label_options: label specific options. ...
We are drawn to easy labels. ... Its a human characteristic to dichotomize things into black and white because it makes things seem so clear. But if you did the real story about a triangle like this, it wouldnt be as interesting. - Elayne Rapping
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ラベルから何番目の命令の直前にブレークポイントを入れられるようにする. 実装した手続きのテストはREPLで試したが,テストの記述は省略. (define (set-breakpoint machine label n) ((machine set-breakpoint) label n)) (define (proc…
Be yourself, even if that means that there isnt a label for you. Explain to anyone who matters who you are. Youre not your labels.
Be yourself, even if that means that there isnt a label for you. Explain to anyone who matters who you are. Youre not your labels.
Ribulose-5-phosphate kinase from maize (Zea mays) can exist in either a reduced, active form or an oxidized, inactive form. Reduced ribulose-5-phosphate kinase is rapidly and irreversibly inactivated by the dichlorotriazine dye Reactive Red 1 (Procion Red MX-2B), but the irreversible inactivation of the oxidized form of ribulose-5-phosphate kinase occurs at only 0.05% of this rate. The rate of inactivation of the reduced enzyme by Reactive Red 1 (apparent bimolecular rate constant 10(4)M-1 X s-1 at pH 7.4 and 25 degrees C) is several orders of magnitude greater than previous estimates of the rates of dye-mediated inactivation of other enzymes. The dye-dependent inactivation of the reduced enzyme is inhibited by Hg2+ or p-mercuribenzoate (thiol reagents that reversibly inhibit ribulose-5-phosphate kinase activity), or by ATP and ADP, the nucleotide substrates of the enzyme. Hydrolysed Reactive Red 1, which does not inactivate the enzyme, is a reversible inhibitor of ribulose-5-phosphate kinase. ...
BioAssay record AID 656250 submitted by ChEMBL: Binding affinity to PS1 Carboxy-terminal fragment in human HeLa cell membrane at 20 nM by photo-affinity in-gel fluorescence method in presence of GSI L458.
The covalent incorporation of [(3)H]all-trans-retinoic acid into proteins has been studied in tumoural Leydig (MLTC-1) cells. The maximum retinoylation activity of MLTC-1 cell proteins was 710+/-29 mean+/-SD) fmoles/8 x 10(4) cells at 37 degrees C. About 90% of [(3)H]retinoic acid was trichloroacetic acid-soluble after proteinase-K digestion and about 65--75% after ...
Suppliers List, E-mail/RFQ Form, Molecular Structure, Weight, Formula, IUPAC, Synonyms for (2-benzoyl-3-methylbenzoyl) 2-benzoyl-3-methylbenzenecarboperoxoate (CAS No. 96662-04-3)
JoVE publishes peer-reviewed scientific video protocols to accelerate biological, medical, chemical and physical research. Watch our scientific video articles.
First things first - most labels from our genre dont pay for tracks, because they cant afford to pay for everything. The best way to set up a label is to do it with friends, that share the vision and have the skills - you need a mastering engineer, a designer and the manager/head of label. And since people are getting more and more into labels and music, the first two wont be that difficult to find. Most engineers/designers work with more than a single label and you already have the label manager ...
I would need to double-check but label should be straight/no formatting/single line text controls while the textarea are rich/with formatting/multi line text controls. Im surprised that carriage return actually work with labels, i would have expected that it made the text disappear from the area for the label (i.e. it would show up if the label control was given more height ...
Even though we rank our top 1,500 labels here, we actually sell items from almost 7,000 record labels. To search thru all record labels, use the search box on the top of the screen ...
By stepping out, you are labeled whether or not you care to claim or accept that label. It is human nature to name and label, no matter if the label is actually true. The safety and security for most comes from the label, not the correctness (or lack thereof) or the label. That which is labeled is safe and predictable and therefore should be boxed appropriately. Left to wander free, the being could change the thoughts of others and even bring out more of those with the same label. This would be considered detrimental because with each newly labeled person, the label changes slightly ...
Girardin Gueuze 1882 (Black Label) is a Gueuze style beer brewed by Brouwerij Girardin in Sint-Ulriks-Kapelle, Belgium. 4.29 average with 1194 ratings, reviews and opinions.
Girardin Gueuze 1882 (Black Label) is a Gueuze style beer brewed by Brouwerij Girardin in Sint-Ulriks-Kapelle, Belgium. 4.29 average with 1199 ratings, reviews and opinions.
Today you are going to learn how supplements arent always what they seem. The label can be deceiving, and could cause you harm.
Diecutter, sheeter Contech Goddard KS USA The Small Format Web-Fed Die-Cutter operates at high speeds. Cycling at 90 hits per minute, the 12 x 16 diecutter is optically registered for cutting accuracy and can thermal die
Kickstarter is an kick-ass way for bands to raise money for projects without relying on a record label. But what if youre a band OUTSIDE the U.S.? Well, then
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C-Box မွာေမးခြန္းေမးသူမ်ားအေနႏွင့္ C-Box ေအာက္က က႑အလိုက္ ခြဲထားတဲ့ ေခါင္းစဥ္ (Labels) ေတြထဲမွာ မိမိသိလိုသည့္ ေရာဂါ (သို႔မဟုတ္) က်န္းမာေရးဆိုင္ရာ ဗဟုသုအေၾကာင္းေရးထားၿပီးသား ပို႔စ္ရွိမရွိ အရင္ရွာၾကည့္ၿပီး ေရးထားၿပီးသားပို႔စ္ မရွိမွသာ C-Box မွာ ေမးပါရန္ ေမတၱာရပ္ခံပါတယ္။ မိမိသိလိုသည့္ အေၾကာင္းအရာကို ပို႔စ္ထဲမွာဖတ္လို႔ မရွင္းလင္းပါက ပို႔စ္ေအာက္က Comment ေနရာမွာ ေမးခြန္း ...
Professor Rudnick is a graduate of Antioch College, where he received a B.S. in Chemistry in 1968. He performed graduate studies in the enzymology of amino acid racemases in the laboratory of Robert H. Abeles in the Graduate Department of Biochemistry at Brandeis University, receiving a Ph.D. in Biochemistry in 1974. His graduate studies led to an understanding of the structure and mechanism of proline racemase that was confirmed by the crystal structure of a homologous protein in 2006. From 1973-1975, Professor Rudnick performed postdoctoral research on lactose permease with H. Ronald Kaback at the Roche Institute of Molecular Biology. This work provided a greater understanding of binding and transport reactions using photoaffinity reagents and substrate analogs. In 1975, he left Roche to become an Assistant Professor in the Department of Pharmacology at Yale, and was promoted to Associate Professor in 1980 and Professor in 1991 ...
Affinity Labels; Animals; Binding, Competitive; Cell Membrane; Electrophoresis, Polyacrylamide Gel; GTP-Binding Proteins; Guanosine 5-O-(3-Thiotriphosphate); Guanylyl Imidodiphosphate; Kinetics; Molecular Weight; Rats; Rats, Inbred Strains; Receptors, Neurokinin-1; Receptors, Neurotransmitter; purification; Submandibular Gland. ...
Find quality suppliers and manufacturers of 6066-82-6(N-Hydroxysuccinimide) for price inquiry. where to buy 6066-82-6(N-Hydroxysuccinimide).Also offer free database of 6066-82-6(N-Hydroxysuccinimide) including MSDS sheet(poisoning, toxicity, hazards and safety),chemical properties,Formula, density and structure, solution etc.
Sicherheitshinweise: P280-P303+P361+P353-P305+P351+P338-P310a Wear protective gloves/protective clothing/eye protection/face protection. IF ON SKIN (or hair): Remove/Take off immediately all contaminated clothing. Rinse skin with water/shower. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER/doctor ...
Preparative-scale photolabeling, isolation of photolabeled Torpedo nAChR fragments, and identification of photolabeled amino acids by automated Edman protein microsequencing were performed as detailed previously (Chiara et al., 2009; Hamouda et al., 2013). In brief, Torpedo nAChR-rich membranes (2 mg of protein/ml; 10 mg of protein and ∼13 nmol ACh binding sites per condition) were photolabeled with 1 μM [3H]CMPI (80 μCi per condition) in the absence and presence of 1 mM Carb, and the nAChR subunits were resolved on an 8% SDS-PAGE gel. The nAChR β, γ, and δ subunits were recovered by passive elution from the gel bands excised from the stained gel and resuspended in digestion buffer (15 mM Tris, 0.5 mM EDTA, 0.1% SDS, pH 8.1). The gel bands containing the [3H]CMPI-photolabeled nAChR α subunit were excised and transferred to 15% polyacrylamide mapping gels and subjected to in-gel digestion with 100 μg of V8 protease to generate nAChR α subunit fragments (αV8-20, αV8-18, αV8-10, and ...
Hello, I am attempting to perform some experiments that inwolve cross-linking with Pierce photoreactive bifunctional cross-linker APDP. As I have no practice using that reagent, any advice would be most welcome. I plan to label one protein via sulphydryl end of APDP and then use the labelled protein in a reaction. After that the glycerol gradient will be run, and appropriate fractions will be flashed with light to activate the photoreactive group of APDP and cross-link that protein to whatever it has in proximity. I have read the papers provided by Pierce, but still have some questions, as follows: - Does APDP decompose thermally readily? Will it be possible to heat APDP-bound protein (that first one) for some 10 min, or will it destroy the photoreactive end (as I am affraid)? And how long can I keep APDP-bound protein sample before actual cross-linking with flashlight? - How photosensitive it is? Do I have to worry about ambient light when labelling the first protein by sulphydryl? - During the ...
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer ...
An apparatus and associated method for applying a label to an article comprising a supporting structure, a label receiver having a receiving face and being adapted to receive and releasably retain a label, and a label dispenser. The label dispenser being adapted to dispense a label onto the receiving face. The dispenser being further adapted to blow a flow of gas against the trailing and lower edges of the label as it is being transferred from the label dispenser onto the receiving face to assist in moving the label onto the receiving face. The receiving face is fitted with a stop plate which prevents the label from being pushed beyond the receiving face and from being mis-positioned. After positioning, the label on the receiving face, the label is applied to the article.
3-[(6-azido-9H-purin-9-yl)methyl]phenylformamide - chemical structural formula, chemical names, chemical properties, synthesis references
You are viewing an interactive 3D depiction of the molecule [[(2S,3R,4R,5R)-5-(6-aminopurine-1,3,7-triium-9-yl)-3-azido-4-hydroxy-tetrahydrofuran-2-yl]methoxy-hydroxy-phosphoryl] phosphono hydrogen phosphate (C10H16N8O12P3) from the PQR.
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Okayama, Japan (PRWEB UK) 5 October 2016 -- The production of proteins at distinct times and locations regulates cell functions, such as cell development and
Development of Novel Affinity Reagents. During the past fiscal year, the Office of Science and Technology Partnerships (OSTP) has continued to support an initia...
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We prepared 1-(4′-azido-2′-deoxy-2′-fluoro-D-arabinofuranosyl)cytosine (10) and its hydrochloride sodium (11) while potential antiviral real estate agents based on the good antiviral information of 4′-substituted nucleosides. (10) (Fig. 1) like a novel potent NRTI that couldovercome drug -resistanceproblems [ 19-21]. Fig. 1 Structures of 1-(4′-Azido-2′-deoxy-2′-fluoro-D-arabinofuranosyl)cytosine (10) and Its Design Lead 4′-Azido-thymidine. 2 Chemistry The synthesis of 10 …Read More. ...
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While the transport of cell surface proteins is relatively easily studied, visualizing the trafficking of intracellular proteins is...
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For the past eight years or so, I have been privileged through this column to share my thoughts, ideas, and observations with the readership of Label & Narrow Web magazine. Each issue has presented me with the challenge of utilizing 1,200 to 1,400 wo
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Chelsea midfielder Frank Lampard believes Rafa Benitez did all that could be expected of him during his difficult period in charge of the club.
Interactions of benzophenone (BP) with the Torpedo nicotinic acetylcholine receptor (nAChR) were characterized by electrophysiological analyses, radioligand binding assays, and photolabeling of nAChR-rich membranes with [3H]BP to identify the amino acids contributing to its binding sites. BP acted as a low potency noncompetitive antagonist, reversibly inhibiting the ACh responses of nAChRs expressed in Xenopus oocytes (IC50 = 600 卩 and the binding of the noncompetitive antagonist [3H]tetracaine to nAChR-rich membranes (IC50 = 150 卩. UV irradiation at 365 nm resulted in covalent incorporation of [3H]BP into the nAChR subunits (d , a ߠ, ?), with photoincorporation limited to the nAChR transmembrane domain. Comparison of nAChR photolabeling in the closed state (absence of agonist) and desensitized state (equilibrated with agonist) revealed selective desensitized state labeling in the d subunit of dPhe-232 in dM1 and dPro-286/dIle-288 near the beginning of dM3 that are within a pocket at the ...
4. A photoreactive polymer comprising a repeating unit represented by the following formula 2a or 2b: ##STR00041## wherein in the formulas 2a and 2b, independently, m is 50 to 5,000; q is an integer from 0 to 4; and at least one of R1, R2, R3 and R4 is a radical represented by the following formula 1a, among the R1 to R4, the remainders other than the radical of the formula 1a are the same as or different from one another and independently selected from the group consisting of hydrogen; halogen; substituted or unsubstituted linear or branched alkyl having 1 to 20 carbon atoms; substituted or unsubstituted linear or branched alkenyl having 2 to 20 carbon atoms; substituted or unsubstituted linear or branched alkynyl having 2 to 20 carbon atoms; substituted or unsubstituted cycloalkyl having 3 to 12 carbon atoms; substituted or unsubstituted aryl having 6 to 40 carbon atoms; substituted or unsubstituted arylalkyl having 5 to 12 carbon atoms; and a polar functional group comprising at least one of ...
Intrigued by similar specificities of the hydra feeding receptor and γ-glutamyl transferase activity toward GSH, we examined the possibility that these two GSH-bihding activities might reside in the same protein. We find that the two activities differ in specificity toward the γ-glutamyl moiety of GSH. The hydra transferase recognizes L-azaserine, L-Glu, D-Glu and L-Gln. The feeding receptor recognizes only L-GlU and L-Gln; L-azaserine and D-Glu have no effect. L-azaserine, known to bind covalently to the γ-glutamyl donor site of mammalian transferase, irreversibly inactivates hydra transferase activity. The transferase affinity label, however, has no effect on the GSH-stimulated feeding response, permitting us to demonstrate that these two activities have different GSH recognition sites and appear to reside in different proteins.. Note: ...
In one aspect, affinity tags for recombinant protein purification are described herein which, in some embodiments, can mitigate or overcome disadvantages of prior affinity tag systems. In some embodiments, for example, affinity tags described herein permit efficient elution of desired recombinant proteins with simplified solution systems, such as alkali metal salt solutions. An affinity tag described herein comprises an amino acid sequence including a repeating amino acid unit of BXXXBXX, wherein B is an amino acid selected from the group consisting of histidine, lysine and arginine and X is an amino acid selected from the group consisting of amino acids other than histidine, lysine and arginine
Opioid Receptor, Peptides, Inhibition, Adenylyl Cyclase, Dynorphin, Cyclization, Kappa Opioid Receptor, Tryptophan, Lead, Esterification, Lysine, Affinity Labels, Concentrations, Damgo, Knowledge, Mu Opioid Receptor, Allylglycine, Concentration, Cyclic Peptides, Cyclizations
Membrane-bound α-bungarotoxin-binding entities derived from rat brain are found to interact specifically with the affinity reagents maleimidobenzyltrimethylammonium (MBTA) and bromoacetylcholine (BAC), originally designed to label nicotinic acetylcholine receptors from electroplax and skeletal muscle. Following treatment of membranes with dithiothreitol, all specffic toxin binding sites are irreversibly blocked by reaction with MBTA or BAC. Affinity reagent labeling of dithiothreitol-reduced membranes is prevented (toxin binding sites are not blocked) by prior alkylation with N-ethylmaleimide, by prior oxidation with dithiobis(2-nitrobenzoic acid), or by incubation with neurotoxin. Reversibly associating cholinergic agonists and antagonists retard the rate of affinity reagent interaction with toxin receptors. The apparent rates of affinity reagent alkylation of toxin receptors, and the influences of other sulfhydryl/disulfide reagents on affinity labeling are comparable to those observed for ...
PMA is a high affinity photoreactive DNA binding dye developed by Biotium. The dye is weakly fluorescent by itself but becomes highly fluorescent upon binding to nucleic acids. It preferentially binds to dsDNA with high affinity. Upon photolysis, the photoreactive azido group on the dye is converted to a highly reactive nitrene radical, which readily reacts with any hydrocarbon moiety at the binding site to form a stable covalent nitrogen-carbon bond, thus resulting in permanent DNA modification. The dye is cell membrane-impermeable and thus can be used to selectively modify DNA from dead cells with compromised membrane integrity, while leaving DNA from viable cells intact. PMA inhibits PCR amplification of modified DNA templates by a combination of removal of modified DNA during purification and inhibition of template amplification by DNA polymerases (see Reference 1 under the references tab). Consequently the dye is useful in the selective detection of viable pathogenic cells by quantitative ...
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The LIFT Fund is the first affinity endowment fund at MCCF, and President and CEO of MCCF Jennifer S. Maddox is excited to be a member of this new affinity fund, which was inspired by board member Carolyn J. Kline. This fund is the culmination of hard work starting in the fall of 2010. Founding donors and members of the LIFT Group include Joan M. Bess, Julie A. Bess, Rosalie Beigh Bonine, Pamela C. Buxton, Anne O. Duff, Carolyn J. Kline, Kathleen Daly Kline, the late Rita Lawson, Kathleen J. Lintner, Jennifer S. Maddox, Nancy Nowalk McKinnis, Jennifer K. Morgan, Ginny Bess Munroe, Emily Payson Ryman, Susan Campbell Thews, and Barbara Winters ...
7-benzoyl-3-methylsulfanyl-1,3-dihydroindol-2-one - chemical structural formula, chemical names, chemical properties, synthesis references
3-(2-Isopropyl-imidazol-1-yl)-propylamine/AFI733756666 can be provided in Alfa Chemistry. We are dedicated to provide our customers the best products and services.
Methods and kits for the quantitation of cellular DNA and cell numbers are provided. Passive element uptake, element-labeled DNA intercalators, and element labeled affinity reagents are used to quantify DNA and cells. The DNA and the cells are analyzed by elemental analysis, including ICP-MS. The methods and kits provide a fast and accurate analysis of cellular DNA and cell numbers.
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Multidrug-resistant cells are thought to maintain low intracellular cytotoxic drug concentration though the active efflux of drugs across the cell membrane. It is presently believed that P-glycoprotein mediates this energy-dependent drug efflux by interacting directly with various lipophilic compounds. In this report, we have used [3H]azidopine in a photoaffinity labeling assay to study the effect of detergents and denaturing agents on P-glycoprotein drug binding in intact cells. Nonionic detergents such as Triton X-100 or Nonidet P-40 at very low concentrations were found to completely abolish azidopine photolabeling to P-glycoprotein and are able to reverse the multidrug resistance phenotype. In contrast, high concentrations of the denaturing agent urea or the zwitterionic detergent 1-[(3-cholamidopropyl)dimethylamino]-1-propanesulfonate did not inhibit azidopine photolabeling to P-glycoprotein. A comparison between verapamil and Triton X-100 revealed that the latter was more effective in ...
Sequence requirements of the ATP-binding site within the C-terminal nucleotide-binding domain (NBD2) of mouse P-glycoprotein were investigated by using two recombinantly expressed soluble proteins of different lengths and photoactive ATP analogues, 8-azidoadenosine triphosphate (8N(3)-ATP) and 2,3,4-O-(2,4,6-trinitrophenyl)-8-azidoadenosine triphosphate (TNP-8N(3)-ATP). The two proteins, Thr(1044)-Thr(1224) (NBD2(short)) and Lys(1025)-Ser(1276) (NBD2(long)), both incorporated the four consensus sequences of ABC (ATP-binding cassette) transporters, Walker A and B motifs, the Q-loop, and the ABC signature, while differing in N-terminal and C-terminal extensions. Radioactive photolabeling of both proteins was characterized by hyperbolic dependence on nucleotide concentration and high-affinity binding with K(0.5)(8N(3)-ATP) = 36-37 microM and K(0.5)(TNP-8N(3)-ATP) = 0.8-2.6 microM and was maximal at acidic pH. Photolabeling was strongly inhibited by TNP-ATP (K(D) = 0.1-5 microM) and ATP (K(D) = 0.5-2.7
Identification of differential expressions of proteins in proteomic profiles of biological samples shows great potential as a valuable technique for the early diagnosis of various diseases. An important challenge in modern protein profiling approaches is to reduce the complexity of the samples by limiting the number of proteins that need to be evaluated for distinction in the expression between normal and deceased cells. In this research, an affinity based approach for the enrichment of nucleotide and nucleoside binding proteins from a complex cell proteome has been developed. To achieve this goal, new N6-biotinylated-8-azido-adenosine probes (AdoRs) have been designed and synthesized to photolabel the nucleotide and nucleoside binding proteins. These probes contain a reactive group that forms a covalent bond with the target proteins, as well as a biotin tag for affinity enrichment using avidin chromatography. Further, a mass spectrometric protein profiling approach is employed to quantitatively

Affinity labeling | definition of affinity labeling by Medical dictionaryAffinity labeling | definition of affinity labeling by Medical dictionary

... affinity labeling explanation free. What is affinity labeling? Meaning of affinity labeling medical term. What does affinity ... Looking for online definition of affinity labeling in the Medical Dictionary? ... Affinity Labelling. (redirected from affinity labeling). Also found in: Encyclopedia. Affinity Labelling. A method for studying ... Affinity labeling , definition of affinity labeling by Medical dictionary https://medical-dictionary.thefreedictionary.com/ ...
more infohttps://medical-dictionary.thefreedictionary.com/affinity+labeling

Affinity label - WikipediaAffinity label - Wikipedia

Affinity labels are molecules similar in structure to a particular substrate for a specific enzyme and are considered to be a ... The label binds covalently to the enzyme so that the substrate can no longer bind, causing a permanent and irreversible change ...
more infohttps://en.wikipedia.org/wiki/Affinity_label

A Photochemical Affinity-Labelling Reagent for the Opiate Receptor(s) | Molecular PharmacologyA Photochemical Affinity-Labelling Reagent for the Opiate Receptor(s) | Molecular Pharmacology

A Photochemical Affinity-Labelling Reagent for the Opiate Receptor(s) Message Subject (Your Name) has forwarded a page to you ... A Photochemical Affinity-Labelling Reagent for the Opiate Receptor(s). BETTY A. WINTER and AVRAM GOLDSTEIN ... A Photochemical Affinity-Labelling Reagent for the Opiate Receptor(s). BETTY A. WINTER and AVRAM GOLDSTEIN ... A Photochemical Affinity-Labelling Reagent for the Opiate Receptor(s). BETTY A. WINTER and AVRAM GOLDSTEIN ...
more infohttp://molpharm.aspetjournals.org/content/8/6/601

Affinity Labeling Detection of Endogenous Receptors from Zebrafish Embryos | Protocol (Translated to Hindi)Affinity Labeling Detection of Endogenous Receptors from Zebrafish Embryos | Protocol (Translated to Hindi)

Molina-Villa, T., Mendoza, V., López-Casillas, F. Affinity Labeling Detection of Endogenous Receptors from Zebrafish Embryos. J ... Identification of a nerve growth factor receptor protein in sympathetic ganglia membranes by affinity labeling. J. Biol. Chem. ... Imaging Glycans in Zebrafish Embryos by Metabolic Labeling and Bioorthogonal Click Chemistry… ...
more infohttps://www.jove.com/video/54405/zebrafish-?language=Hindi

Triazine dyes, a new class of affinity labels for nucleotide-dependent enzymes | Biochemical JournalTriazine dyes, a new class of affinity labels for nucleotide-dependent enzymes | Biochemical Journal

Triazine dyes, a new class of affinity labels for nucleotide-dependent enzymes. Y D Clonis, C R Lowe ... Triazine dyes, a new class of affinity labels for nucleotide-dependent enzymes ... Triazine dyes, a new class of affinity labels for nucleotide-dependent enzymes ... Triazine dyes, a new class of affinity labels for nucleotide-dependent enzymes ...
more infohttp://www.biochemj.org/content/191/1/247

Decrease of Metastatogenic Potential by Pregraft Treatment of Lewis Lung Carcinoma Cells with Proteinase and Protein Kinase...Decrease of Metastatogenic Potential by Pregraft Treatment of Lewis Lung Carcinoma Cells with Proteinase and Protein Kinase...

Metastatogenic Potential by Pregraft Treatment of Lewis Lung Carcinoma Cells with Proteinase and Protein Kinase Affinity Labels ... Metastatogenic Potential by Pregraft Treatment of Lewis Lung Carcinoma Cells with Proteinase and Protein Kinase Affinity Labels ... Metastatogenic Potential by Pregraft Treatment of Lewis Lung Carcinoma Cells with Proteinase and Protein Kinase Affinity Labels ... Metastatogenic Potential by Pregraft Treatment of Lewis Lung Carcinoma Cells with Proteinase and Protein Kinase Affinity Labels ...
more infohttp://cancerres.aacrjournals.org/content/45/11_Part_1/5335

An affinity label for the regulatory dithiol of ribulose-5-phosphate kinase from maize (Zea mays) | Biochemical JournalAn affinity label for the regulatory dithiol of ribulose-5-phosphate kinase from maize (Zea mays) | Biochemical Journal

An affinity label for the regulatory dithiol of ribulose-5-phosphate kinase from maize (Zea mays). A R Ashton ... This inhibition is competitive with respect to ATP (Ki approximately 0.5 mM). The dye appears to act as an affinity label for ... An affinity label for the regulatory dithiol of ribulose-5-phosphate kinase from maize (Zea mays) ... An affinity label for the regulatory dithiol of ribulose-5-phosphate kinase from maize (Zea mays) ...
more infohttp://www.biochemj.org/content/217/1/79

The Synthesis of DAMGO-Based Potential Affinity Labels with High Mu Opioid Receptor Affinity and the Formation of Cyclic O...The Synthesis of DAMGO-Based Potential Affinity Labels with High Mu Opioid Receptor Affinity and the Formation of Cyclic O...

Opioid Receptor Peptide Affinity Affinity Label Protected Peptide Parent Ligand This is a preview of subscription content, log ... The Synthesis of DAMGO-Based Potential Affinity Labels with High Mu Opioid Receptor Affinity and the Formation of Cyclic O- ... The Synthesis of DAMGO-Based Potential Affinity Labels with High Mu Opioid Receptor Affinity and the Formation of Cyclic O- ...
more infohttps://rd.springer.com/chapter/10.1007/978-0-387-73657-0_119

Wellcome Library | Affinity labelling and cloning of steroid and thyroid hormone receptorsWellcome Library | Affinity labelling and cloning of steroid and thyroid hormone receptors

Affinity labelling and cloning of steroid and thyroid hormone receptors Author. *Gronemeyer, H ...
more infohttps://wellcomelibrary.org/item/b18029310

Steroid derivatives for electrophilic affinity labelling of glucocorticoid binding sites: interaction with the glucocorticoid...Steroid derivatives for electrophilic affinity labelling of glucocorticoid binding sites: interaction with the glucocorticoid...

The affinity of DXM-M for the glucocorticoid receptor, measured by competitive binding assay, was 1/15 that of DXM. ... Polyacrylamide gel electrophoresis in sodium dodecyl sulphate of the [3H]-DXM-M labeled glucocorticoid receptor revealed a ... 6 cells and it acts as a slowly reversible glucocorticoid agonist at concentrations which correlate with the affinity of DXM-M ... To investigate the possible use of electrophilic affinity labelling for the characterization of glucocorticoid receptors, ...
more infohttps://www.semanticscholar.org/paper/Steroid-derivatives-for-electrophilic-affinity-of-Weisz-Buzard/f0c06d0e0c1e4bffbabe5be72ec6b8808b17d902

A simple photo-affinity labeling protocol<...A simple photo-affinity labeling protocol<...

"A simple photo-affinity labeling protocol",. abstract = "After photo-crosslinking and proteolysis of a photoaffinity labeled ... Li HY, Liu Y, Fang K, Nakanishi K. A simple photo-affinity labeling protocol. Chemical Communications. 1999 Feb 21;(4):365-366. ... Li, HY, Liu, Y, Fang, K & Nakanishi, K 1999, A simple photo-affinity labeling protocol, Chemical Communications, no. 4, pp. ... Li, H. Y., Liu, Y., Fang, K., & Nakanishi, K. (1999). A simple photo-affinity labeling protocol. Chemical Communications, (4), ...
more infohttps://arizona.pure.elsevier.com/en/publications/a-simple-photo-affinity-labeling-protocol

Identification and extraction of the Na+/D-glucose co-transporter (SGLT1)-related polypeptide using affinity labeling and...Identification and extraction of the Na+/D-glucose co-transporter (SGLT1)-related polypeptide using affinity labeling and...

Identification and extraction of the Na+/D-glucose co-transporter (SGLT1)-related polypeptide using affinity labeling and ... related polypeptide using affinity labeling and immunoprecipitation. ... related polypeptide using affinity labeling and immunoprecipitation ...
more infohttp://pubman.mpdl.mpg.de/pubman/faces/viewItemOverviewPage.jsp?itemId=escidoc:1833666

Synthesis and pre-clinical evaluation of a new class of high-affinity 18F-labeled PSMA ligands for detection of prostate cancer...Synthesis and pre-clinical evaluation of a new class of high-affinity 18F-labeled PSMA ligands for detection of prostate cancer...

... mainly features 68Ga-labeled tracers, notably [68Ga]Ga-PSMA-HBED-CC. The longer half-life of... ... Synthesis and pre-clinical evaluation of a new class of high-affinity 18F-labeled PSMA ligands for detection of prostate cancer ... Click labelling in PET radiochemistry. J Label Compd Radiopharm. 2009;52:407-14.CrossRefGoogle Scholar ... We aimed to develop high-affinity PSMA inhibitors labeled with fluorine-18 as alternative tracers for prostate cancer. ...
more infohttps://link.springer.com/article/10.1007%2Fs00259-016-3556-5

Affinity labeling of Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase with the 2′,3′-dialdehyde derivative of ATP<...Affinity labeling of Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase with the 2′,3′-dialdehyde derivative of ATP<...

Affinity labeling of Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase with the 2′,3′-dialdehyde derivative of ATP. / ... Affinity labeling of Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase with the 2′,3′-dialdehyde derivative of ATP. En ... Affinity labeling of Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase with the 2′,3′-dialdehyde derivative of ATP. ... T1 - Affinity labeling of Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase with the 2′,3′-dialdehyde derivative of ...
more infohttps://researchers.unab.cl/es/publications/affinity-labeling-of-saccharomyces-cerevisiae-phosphoenolpyruvate

Novel Gpr39 Agonists: Correlation Of Binding Affinity Using Label-Free Back-Scattering Interferometry With Potency In...Novel Gpr39 Agonists: Correlation Of Binding Affinity Using Label-Free Back-Scattering Interferometry With Potency In...

Novel Gpr39 Agonists: Correlation Of Binding Affinity Using Label-Free Back-Scattering Interferometry With Potency In ... The difficulty associated with obtaining small molecule affinity data for functionally intact GPCRs effectively restricts the ... Back-scattering interferometry (BSI) is an emerging label-free, conformation-sensitive detection technology for quantitative ... The ability to measure the affinity of small molecule agonists such as Zn2+is especially novel, given the unfavorable mass ...
more infohttps://www.technologynetworks.com/tn/posters/novel-gpr39-agonists-correlation-of-binding-affinity-using-labelfree-backscattering-interferometry-with-potency-in-functional-assays-229694

Affinity Ranking | Label-free Agile R100 Kinetic Characterization SystemAffinity Ranking | Label-free Agile R100 Kinetic Characterization System

Graphene biosensor Agile R100 provides an 11-log dynamic range to enable accurate and reliable affinity ranking for hit ... Affinity ranking with a label-free kinetic binding platform can increase the efficiency of the drug discovery process. ... The affinity (KD) of each TNFα and inhibitor interaction calculated by Agile R100 confirms the previously-reported IC50 rank ... Agile R100 has an 11-log dynamic range, letting you use a single platform to measure very high affinity antibodies as well as ...
more infohttps://www.nanomedicaldiagnostics.com/affinity-ranking/

AID 598215 - Binding affinity to recombinant 15N-labelled PSD95-PDZ1 domain expressed in Escherichia coli BL21 assessed as...AID 598215 - Binding affinity to recombinant 15N-labelled PSD95-PDZ1 domain expressed in Escherichia coli BL21 assessed as...

Binding affinity to recombinant 15N-labelled PSD95-PDZ1 domain expressed in Escherichia coli BL21 assessed as chemical shift ...
more infohttps://pubchem.ncbi.nlm.nih.gov/bioassay/598215

An Electron Paramagnetic Resonance Method for Measuring the Affinity of a Spin-Labeled Analog of Cholesterol for Phospholipids,...An Electron Paramagnetic Resonance Method for Measuring the Affinity of a Spin-Labeled Analog of Cholesterol for Phospholipids,...

"An Electron Paramagnetic Resonance Method for Measuring the Affinity of a Spin-Labeled Analog of Cholesterol for Phospholipids ... An Electron Paramagnetic Resonance Method for Measuring the Affinity of a Spin-Labeled Analog of... Williams, Justin; Wassall, ... An Electron Paramagnetic Resonance Method for Measuring the Affinity of a Spin-Labeled Analog of Cholesterol for Phospholipids ... An Electron Paramagnetic Resonance Method for Measuring the Affinity of a Spin-Labeled Analog of Cholesterol for Phospholipids ...
more infohttps://www.deepdyve.com/lp/springer_journal/an-electron-paramagnetic-resonance-method-for-measuring-the-affinity-Fw5KhsMUK0

Differential Processing of Autoantigens in Lysosomes from Human Monocyte-Derived and Peripheral Blood Dendritic Cells | The...Differential Processing of Autoantigens in Lysosomes from Human Monocyte-Derived and Peripheral Blood Dendritic Cells | The...

Affinity labeling of active proteases. For labeling of active cysteine proteases, cell lysates (5 μg) were incubated with ... the affinity-labeling type of experiments with the CatG-reactive affinity probe DAP (30) were performed (Fig. 7⇓b). A strong ... Activity-based affinity labeling using the active site-directed chemical probe DCG-0N was used to differentiate between the ... In addition, we failed to detect CatL and/or CatC in CD1c-DC using the affinity-labeling technique, in contrast to MO-DC, where ...
more infohttps://www.jimmunol.org/content/175/9/5940?ijkey=59ddf579bad8461546cb1f0c567fad26bee7fc83&keytype2=tf_ipsecsha

3H]A-317491, a Novel High-Affinity Non-Nucleotide Antagonist That Specifically Labels Human P2X2/3 and P2X3 Receptors | Journal...3H]A-317491, a Novel High-Affinity Non-Nucleotide Antagonist That Specifically Labels Human P2X2/3 and P2X3 Receptors | Journal...

Under these conditions, [3H]A-317491 also labeled high-affinity (Kd = 9 nM) binding sites. Lower affinity binding components (K ... A-317491 specifically labeled high-affinity (Kd = 0.9 nM) recognition sites. High-affinity [3H]A-317491 binding was not ... 3H]A-317491, a Novel High-Affinity Non-Nucleotide Antagonist That Specifically Labels Human P2X2/3 and P2X3 Receptors. Michael ... 3H]A-317491, a Novel High-Affinity Non-Nucleotide Antagonist That Specifically Labels Human P2X2/3 and P2X3 Receptors. Michael ...
more infohttp://jpet.aspetjournals.org/content/310/1/407

Rabbit anti human antiplasmin, affinity purified, biotin labeledRabbit anti human antiplasmin, affinity purified, biotin labeled

... biotin labeled - Biotin labeled polyclonal antibody. Affinity purified by immobilized human antiplasmin. ... Rabbit anti human antiplasmin, affinity purified, biotin labeled. Biotin labeled polyclonal antibody (host rabbit). Affinity ... All Products / Polyclonal Antibodies / Anti Human Targets / Rabbit anti human antiplasmin, affinity purified, biotin labeled. ... Rabbit anti human antithrombin III IgG fraction, HRP labeled. $355 - $2,830 Cat #: ASHATIII-GF-HRPSelect options ...
more infohttps://mol-innov.com/products/rabbit-anti-human-antiplasmin-affinity-purified-biotin-labeled/

Word of Mouth: Jameel Bruner of The Internet Has Worldly Taste And An Affinity For Anime | Green LabelWord of Mouth: Jameel Bruner of The Internet Has Worldly Taste And An Affinity For Anime | Green Label

Word of Mouth is a series on Green Label wherein we get you guys onto cool things, via cool people. Jameel Bruner of The ... Check The Label: Get Ready for the Waves of Coastal.... When big brands lost that special something, GreyxWaves tried to ... Word of Mouth: Jameel Bruner of The Internet Has Worldly Taste And An Affinity For Anime ... Watch the Trailer for Last Days of JKWON, Green Labels.... Skaters know no boundaries. ...
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Ethylene Rapidly Up-Regulates the Activities of Both Monomeric GTP-Binding Proteins and Protein Kinase(s) in Epicotyls of Pea |...Ethylene Rapidly Up-Regulates the Activities of Both Monomeric GTP-Binding Proteins and Protein Kinase(s) in Epicotyls of Pea |...

Affinity Labeling with [α-32P]GTP. Affinity labeling of G-proteins was carried out according to the method of Löw et al. (1992) ... 1992) Affinity labeling of GTP-binding proteins in cellular extracts. FEBS Lett 303:64-68. ... After labeling, the proteins were precipitated with 80% (v/v) acetone at −20°C and pelleted by centrifugation. The pellets were ... Labeled proteins were resolved using SDS-PAGE according toLaemmli (1970) or two-dimensional electrophoresis. Bio-Rad Mini- ...
more infohttp://www.plantphysiol.org/node/23738.full.print

Meir Wilchek - WikipediaMeir Wilchek - Wikipedia

Affinity labeling[edit]. Affinity label is a molecule that is similar in structure to a particular substrate for a specific ... for affinity chromatography, affinity labeling, affinity therapy, and the avidin-biotin system. The Avidin-biotin complex is ... Specific uses of affinity chromatography include antibody affinity, Immobilized metal ion affinity chromatography and ... Affinity chromatography[edit]. Affinity chromatography[6] is a method of separating biochemical mixtures, based on a highly ...
more infohttps://en.wikipedia.org/wiki/Meir_Wilchek

Use of real-time, label-free analysis in revealing low-affinity binding to blood group antigens by Helicobacter pyloriUse of real-time, label-free analysis in revealing low-affinity binding to blood group antigens by Helicobacter pylori

... Fei, Y ... OI-RD microscopy also confirmed the high-affinity binding of H. pylori outer-membrane protein BabA to Le(b). The OI-RD ... low-affinity microbial attachment mechanisms that may be essential for infectious processes. OI-RD microscopy was used to ... to immobilized glycoconjugates in real time with no need for labeling tags. The results suggest the presence of additional ...
more infohttp://umu.diva-portal.org/smash/record.jsf?pid=diva2:661810
  • Back-scattering interferometry (BSI) is an emerging label-free, conformation-sensitive detection technology for quantitative mass- and matrix-independent biophysical characterization of small molecule interaction with complex drug target proteins under native-like conditions (1). (technologynetworks.com)
  • We present an experimental system, oblique-incidence reflectivity difference (OI-RD) microscopy, which allows the detection of novel, low-affinity microbial attachment mechanisms that may be essential for infectious processes. (diva-portal.org)
  • Chemistry in living cells: detection of active proteasomes by a two-step labeling strategy. (semanticscholar.org)
  • The effectiveness of a photolabel with light of wavelength 365 nm depends on the labeling amino acid (L-4'-nitrophenylalanine, L-4'-diazoniumphenylalanine, or L-4'-azidophenylalanine) and on its position in the peptide (replacing Tyr4 and/or Phe8). (mendeley.com)
  • Aspartyl peptide labeled by 2-(4-bromo-2,3-dioxobutylthio)adenosine 5'-diphosphate in the allosteric ADP site of pig heart NAD+-dependent isocitrate dehydrogenase. (nih.gov)
  • Steric compatibility of the rigid steroid moiety for ordered saturated chains contributes to the high affinity that holds chol and sphingomyelin together in lipid rafts whereas, conversely, poor affinity of the sterol for highly disordered polyunsaturated fatty acids (PUFAs) is hypothesized to drive the formation of PUFA-containing phospholipid domains depleted in chol. (deepdyve.com)
  • A radioactive analogue of levorphanol, [ 3 H] N -β-( p -azidophenyl)ethylnorlevorphanol (compound 6 ), has been synthesized as a photochemical affinity label for the opiate receptor site(s). (aspetjournals.org)
  • Monochlorotriazine dyes, including Cibacron Blue F3G-A, do not inactivate lactate dehydrogenase but display high affinity and thus inhibit the inactivation by dichlorotriazine dyes. (biochemj.org)
  • The results indicate that oATP can be used as an affinity label for yeast phosphoenolpyruvate carboxykinase. (unab.cl)
  • Partition coefficients ( $$ K_{\text{B}}^{\text{A}} $$ K B A ) between lipids derived from our results for chlstn agree with values obtained for chol and confirm that decreased affinity for the sterol accompanies increasing acyl chain unsaturation. (deepdyve.com)
  • Biodistribution studies of the two tracers with the highest imaged-derived tumor uptake and highest PSMA affinity were undertaken at 1 h, 2 h and 4 h post-injection (p.i.), and co-administration of PMPA was used to determine whether uptake was PSMA-specific. (springer.com)