Phylogenetic analysis of the genus Aeromonas based on two housekeeping genes. (1/55)

The phylogenetic relationships of all known species of the genus Aeromonas, and especially Aeromonas bestiarum and Aeromonas salmonicida, were investigated on 70 strains using the rpoD sequence, which encodes the sigma70 factor. This analysis was complemented with the sequence of gyrB, which has already proven useful for determining the phylogenetic relationships in the genus. Nucleotide sequences of rpoD and gyrB showed that both genes had similar substitution rates (< 2 %) and a similar number of variable positions (34 % for rpoD versus 32 % for gyrB). Strain groupings by analysis of rpoD, gyrB and a combination of both genes were consistent with the taxonomic organization of all Aeromonas species described to date. However, the simultaneous analysis of both clocks improved the reliability and the power to differentiate, in particular, closely related taxa. At the inter-species level, gyrB showed a better resolution for differentiating Aeromonas sp. HG11/Aeromonas encheleia and Aeromonas veronii/Aeromonas culicicola/Aeromonas allosaccharophila, while rpoD more clearly differentiated A. salmonicida from A. bestiarum. The analysis of rpoD provided initial evidence for clear phylogenetic divergence between the latter two species.  (+info)

Structural studies of the capsular polysaccharide and lipopolysaccharide O-antigen of Aeromonas salmonicida strain 80204-1 produced under in vitro and in vivo growth conditions. (2/55)

Aeromonas salmonicida is a pathogenic aquatic bacterium and the causal agent of furunculosis in salmon. In the course of this study, it was found that when grown in vitro on tryptic soy agar, A. salmonicida strain 80204-1 produced a capsular polysaccharide with the identical structure to that of the lipopolysaccharide O-chain polysaccharide. A combination of 1D and 2D NMR methods, including a series of 1D analogues of 3D experiments, together with capillary electrophoresis-electrospray MS (CE-ES-MS), compositional and methylation analyses and specific modifications was used to determine the structure of these polysaccharides. Both polymers were shown to be composed of linear trisaccharide repeating units consisting of 2-acetamido-2-deoxy-D-galacturonic acid (GalNAcA), 3-[(N-acetyl-L-alanyl)amido]-3,6-dideoxy-D-glucose[3-[(N-acetyl-L-alanyl)amido]-3 -deoxy-D-quinovose, Qui3NAlaNAc] and 2-acetamido-2,6-dideoxy-D-glucose (2-acetamido-2-deoxy-D-quinovose, QuiNAc) and having the following structure: [-->3)-alpha-D-GalpNAcA-(1-->3)-beta-D-QuipNAc-(1-->4)-beta-D-Quip3NAlaNAc-(1-]n, where GalNAcA is partly presented as an amide and AlaNAc represents N-acetyl-L-alanyl group. CE-ES-MS analysis of CPS and O-chain polysaccharide confirmed that 40% of GalNAcA was present in the amide form. Direct CE-ES-MS/MS analysis of in vivo cultured cells confirmed the formation of a novel polysaccharide, a structure also formed in vitro, which was previously undetectable in bacterial cells grown within implants in fish, and in which GalNAcA was fully amidated.  (+info)

Genetic diversity among A-proteins of atypical strains of Aeromonas salmonicida. (3/55)

The virulence array protein gene A (vapA) encoding the A-protein subunit of the surface layer of 23 typical and atypical strains of Aeromonas salmonicida from salmonids and marine fish species were sequenced, and the deduced A-protein sequences compared. The A-proteins of the typical A. salmonicida ssp. salmonicida strains were shown to be identical, while amino acid variability was revealed among A-proteins of atypical strains. The highest amino acid variability appears to be in a predicted surface exposed region and is believed to result in antigenic differences among the atypical strains of A. salmonicida.  (+info)

A deterministic model for the dynamics of furunculosis in chinook salmon Oncorhynchus tshawytscha. (4/55)

Studies were undertaken to determine the parameters of transmission of Aeromonas salmonicida in chinook salmon Oncorhynchus tshawytscha, and to develop a deterministic model of the dynamics of experimental furunculosis. For determination of disease transmission coefficient (beta), disease-related mortality rate (alpha) and natural mortality rate (gamma), fish in 70 tanks (approximately 42 fish tank(-1)) were each exposed to a single infectious donor fish, 7 tanks were randomly selected daily and all individuals were examined for the presence of A. salmonicida in the kidney. The proportion of susceptible (S), infected (I) and removed (R, dead) individuals were determined daily. The parameters beta, alpha, gamma, reproductive ratio (R0) and threshold density were estimated to be 0.0214 infected ind. d(-1), 0.29 infected ind. d(-1), 0.00015 ind. d(-1), 3.23 and 13.56 ind., respectively. Using these parameters, a deterministic disease model of A. salmonicida infection as a cause of furunculosis was constructed. The net rate at which new individuals became infected (the incidence rate) per unit time was proportional to S x I x beta. The model-produced data for S were significantly associated with experimental data (r2 = 0.92). In brief, a simple SIR (susceptible-infected-removed) model was successfully utilized to simulate observed data  (+info)

Attenuated virulence of an Aeromonas salmonicida subsp. salmonicida type III secretion mutant in a rainbow trout model. (5/55)

Aeromonas salmonicida subsp. salmonicida is the causative agent of furunculosis, a severe systemic disease affecting salmonid fish. This bacterium contains a type III protein secretion system that is responsible for the secretion and translocation of the ADP-ribosylating toxin, AexT, into the cytosol of fish cells. This study showed that inactivation of the type III secretion system by marker-replacement mutagenesis of the gene ascV, which encodes an inner-membrane component of the type III secretion system, attenuated virulence in a rainbow trout model. The isogenic ascV deletion mutant was phagocytosed by peripheral blood leukocytes but the wild-type (wt) A. salmonicida subsp. salmonicida isolate was not. Histological examination of fish experimentally infected with the wt bacterium revealed extensive tissue necrosis and bacterial aggregates in all organs examined, including the heart, kidney and liver, indicating that the isolate established a systemic infection. Cumulative mortality of fish experimentally infected with the wt bacterium reached 88%. In contrast, no mortality was observed among fish infected with the same dose of the ascV mutant, and histological examination of fish infected with this strain revealed healthy organs. The results indicate that the type III secretion system of A. salmonicida subsp. salmonicida is required to establish systemic infection.  (+info)

Standardization of a broth microdilution susceptibility testing method to determine minimum inhibitory concentrations of aquatic bacteria. (6/55)

A multiple laboratory study was conducted in accordance with the standards established by the Clinical and Laboratory Standards Institute (CLSI), formerly the National Committee for Clinical Laboratory Standards (NCCLS), for the development of quality control (QC) ranges using dilution antimicrobial susceptibility testing methods for bacterial isolates from aquatic animal species. QC ranges were established for Escherichia coli ATCC 25922 and Aeromonas salmonicida subsp. salmonicida ATCC 33658 when testing at 22, 28 and 35 degrees C (E. coli only) for 10 different antimicrobial agents (ampicillin, enrofloxacin, erythromycin, florfenicol, flumequine, gentamicin, ormetoprim/sulfadimethoxine, oxolinic acid, oxytetracycline and trimethoprim/sulfamethoxazole). Minimum inhibitory concentration (MIC) QC ranges were determined using dry- and frozen-form 96-well plates and cation-adjusted Mueller-Hinton broth. These QC ranges were accepted by the CLSI/NCCLS Subcommittee on Veterinary Antimicrobial Susceptibility Testing in January 2004. This broth microdilution testing method represents the first standardized method for determining MICs of bacterial isolates whose preferred growth temperatures are below 35 degrees C. Methods and QC ranges defined in this study will enable aquatic animal disease researchers to reliably compare quantitative susceptibility testing data between laboratories, and will be used to ensure both precision and inter-laboratory harmonization.  (+info)

Metabolic changes in Atlantic salmon exposed to Aeromonas salmonicida detected by 1H-nuclear magnetic resonance spectroscopy of plasma. (7/55)

1H-NMR (nuclear magnetic resonance)-based chemometric methods have been applied for the first time to investigate changes in the plasma metabolite profiles of Atlantic salmon Salmo salar as a result of exposure to Aeromonas salmonicida subsp. salmonicida, a Gram-negative bacterium that is the etiological agent of furunculosis. Plasma samples were obtained from salmon that survived 21 d post exposure to A. salmonicida, and from a control group maintained under similar conditions. 1D 1H-NMR spectra were acquired and principal components analysis (PCA) was used to assess differences between the spectral profiles of plasma from salmon that survived an A. salmonicida challenge, and non-infected controls. PCA enables simultaneous comparison of spectra, presenting a simplified overview of the relationship between spectral data, where spectra cluster based on metabolite profile similarities and differences; information regarding the metabolite variations can therefore be readily deciphered. The major metabolite changes responsible for the spectral differences were related to modification in the lipoprotein profile and choline-based residues, with minor changes in carbohydrates, glycerol, trimethylamine-N-oxide and betaine. These changes indicated that exposure to A. salmonicida induced a characteristic biochemical response which could be used to determine the health status of salmon. This study suggests that with further development this metabolite profiling technique may be a useful tool for diagnosis of disease states in salmon and could provide a better understanding of the host-pathogen relationship which at present is poorly understood for A. salmonicida and Atlantic salmon.  (+info)

Characterisation of atypical Aeromonas salmonicida infection in Arctic charr Salvelinus alpinus and European grayling Thymallus thymallus. (8/55)

Cultured stocks of Arctic charr Salvelinus alpinus and European grayling Thymallus thymallus are vulnerable to infection by achromogenic atypical Aeromonas salmonicida (AAS). In Finland, natural stocks of both fish species have to be supported by restocking, and AAS infection poses a threat to successful restocking because no preventive means are available. In this study, we analysed AAS isolates from Arctic charr and European grayling and from other sources genetically, and characterised the signs and pathology of AAS infection in Arctic charr and European grayling both under farming conditions and after experimental challenge. AAS outbreaks were recorded in 1 fish farm over an 8 yr period. Among various salmonid fishes under farming conditions, only Arctic charr and European grayling were susceptible to AAS infection. The disease caused by AAS could be reproduced in both species using the same AAS strain in an experimental challenge. The course of the disease and pathology of natural and experimental AAS infection differed between the 2 species, even though only 1 strain was used for challenge. Isolates of AAS from Arctic charr and European grayling were genetically identical within a single river water basin. However, genetic heterogeneity was observed among the isolates from different water basins. In both species, AAS caused systemic infection. The results suggest that the same AAS strain could be used to develop a vaccine to protect both Arctic charr and European grayling from AAS infection.  (+info)

This review summarizes the results of studies on atypical Aeromonas salmonicida (aAS) -infection among farmed Arctic charr (Salvelinus alpinus) and European grayling (Thymallus thymallus) (later referred as charr and grayling, respectively). Specifically, I aimed to characterize the causative agent of aAS -infection, to describe putative virulence factors of the bacteria and cross pathology of the infection in charr and grayling. I also aimed to describe some of the immune defense functions in charr. I also described a concomitant presence of a common fresh water fish parasite, Diplostomum spathaceum, and aAS -bacteria in manifestation of the bacterial infection. The experimental juvenile charr and grayling, originated from Vuoksi, Kymijoki and Inari watercourses in Finland. Several (n=66) aAS isolates from 7 watercourses in Finland and from the Baltic sea were characterized in the study. These were compared to 11 Swedish, 1 Icelandic aAS isolates, to 3 A. salmonicida subsp. salmonicida (ASS) ...
Aeromonas salmonicida subsp. salmonicida ATCC ® 33658™ Designation: NCMB 1102 TypeStrain=True Application: Quality control strain Susceptibility disc testing Testing
In this study we have examined the levels of protection against infection with a Danish strain of A. salmonicida subsp. salmonicida in both non-vaccinated, as well as vaccinated rainbow trout. Both a commercial vaccine (AlphaJect 3000, PHARMAQ AS) as well as an experimental auto-vaccine was tested. For comparison, the isolated adjuvant used in the commercial vaccine, as well as the one used in the experimental vaccine was included in the experimental setup. The protective effects of the vaccines were tested by bacterial challenge 18 weeks post vaccination, and during the 18 weeks, the development of specific antibodies was monitored using ELISA assays ...
The study was cancelled mid-way through its schedule when we learned that the fish used to putatively recover EEDV from archived material carried an atypical Aeromonas salmonicida infection whose clinical presentation mimicked EED. We had relied on clinical presentation as an indicator of EEDV infection. Subsequent challenges of young-of-the-year lake trout from another source were unsuccessful in producing EEDV infection. Thus, while we thought we had recovered EEDV from archived material, in fact, we had not and did not have the clinical material to continue the study. ...
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Abstract: In vitro susceptibilities of 30 Aeromonas salmonicida strains isolated from rainbow trout (Oncorhynchus mykiss) were determined against to 23 antimicrobials by using disc diffusion method. According to antimicrobial susceptibility tests results, A. salmonicida strains were found susceptible to all antibiotics tested except for the ampicilin and vancomycine. The major fatty acids, used as indicators for identification of the bacteria, were found as 14:0 3OH/16:1 ISO I, 16:1 w7c/15 ISO 2OH, 16:0 and 18:1 w7c, respectively. ...
Aeromonas salmonicida has been recognized as a pathogen of fish for over 100 years. In 1894 Emmerich and Weibel made the first report of its isolation during a disease outbreak at a Bavarian brown trout hatchery. The manifestations of the disease include furuncle-like swelling and, at a later stage, ulcerative lesions on infected trout. Since that time a number of subspecies of Aeromonas salmonicida have been recognized, although the taxonomy of the species is far from settled. While Aeromonas salmonicida was traditionally thought of as a pathogen of salmonids, global reports now confirm that this pathogen has been associated with clinical or covert disease in a variety of salmonid and non-salmonid species in freshwater, brackish water and sea water. Aeromonas salmonicida (strain A449) was isolated from a brown trout in the Eure River, France in 1975 and harbors one chromosome and 5 plasmids. Comparison to the related A.hydrophilia ATCC 7966 (AERHH) show the presence of numerous insertion ...
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Mariculture in Denmark is based on production of rainbow trout grown two years in fresh water followed by one growth season in sea cages. Although the majority of rainbow trout are vaccinated against the most serious bacterial pathogens - Aeromonas salmonicida subsp. salmonicida, Vibrio anguillarum and Yersinia ruckeri, by the use of commercially available vaccines, disease outbreaks requiring treatment with antibiotics still occur. The present study tested the potential of a new experimental multicomponent vaccine that is based on local bacterial strains, isolated from rainbow trout in Danish waters, and thus custom-designed for Danish rainbow trout mariculture. The vaccination with the multicomponent vaccine resulted in protection against three relevant bacterial diseases (yersiniosis, furunculosis, vibriosis) under experimental conditions. We showed that i.p. injection of the vaccine induced specific antibody responses in trout against the different bacterial antigens and regulated expression ...
The results of this study revealed that a bacterial communication system termed quorum sensing is involved in regulation of bacterial pathogenicity and production of AsaP1, cytotoxic activity, and a brown pigment secreted by the bacterium. A RT-qPCR based analysis of the innate and adaptive immune response in Arctic charr showed that the immune response triggered by a wild type strain and an AsaP1-deletion strain was significantly different, indicating the importance of AsaP1 in the development of the host defense. Mutants of AsaP1 lacking toxic, but retaining immunogenic properties of the native enzyme, were constructed and transformed into the wild type strain. A bacterin based on a mutant of the bacterium secreting an AsaP1-toxoid was used to vaccinate Arctic charr and found to induce good protection against atypical furunculosis. The results add to the understanding of the pathogen and its interaction with Arctic charr during infection and immunization.. Um doktorsefnið. Johanna Mareile ...
Pharmacokinetics describes the time course of drug absorption, distribution, metabolism and excretion. Pharmacodynamics is the relationship between unbound drug concentration over time and the resulting antimicrobial effect. Pharmacokinetic/ pharmacodynamic (PK/PD) indices quantify the relationship between pharmacokinetic parameters (i.e., area under the concentration-time curve, AUC) and microbiological parameters (i.e., minimal inhibitory concentrations, MICs), and are used to establish interpretive criteria or clinical breakpoints. The three primary PK/PD indices used are the AUC over 24 h at steady-state/MIC (AUCss/MIC), the peak concentration/MIC (Cmax/MIC), and the percentage of time over 24 hours that the drug concentration exceeds the MIC at steady-state pharmacokinetic conditions (T,MIC). These indices can be used to determine both appropriate dosage regimens and index magnitudes required for efficacy and reduced antimicrobial resistance emergence. The goal of this work was to determine ...
Phage therapy may represent a viable alternative to antibiotics to inactivate fish pathogenic bacteria. Its use, however, requires the awareness of novel kinetics phenomena not applied to conventional drug treatments. The main objective of this work was to isolate bacteriophages with potential to inactivate fish pathogenic bacteria, without major effects on the structure of natural bacterial communities of aquaculture waters. The survival was determined in marine water, through quantification by the soft agar overlay technique. The host specificity was evaluated by cross infection. The ecological impact of phage addition on the structure of the bacterial community was evaluated by DGGE of PCR amplified 16S rRNA gene fragments. The survival period varied between 12 and 91 days, with a higher viability for Aeromonas salmonicida phages. The phages of Vibrio parahaemolyticus and of A. salmonicida infected bacteria of different families with a high efficacy of plating. The specific phages of pathogenic
NIH Funding Opportunities and Notices in the NIH Guide for Grants and Contracts: ImmuneChip: Engineering Microphysiological Immune Tissue Platforms (U01 Clinical Trial Not Allowed) PAR-19-138. NIBIB
BACKGROUND: Transmitted HIV-1 clade B or C R5 viruses have been reported to infect macrophages inefficiently, while other studies have described R5 viruses in late disease with either an enhanced macrophage-tropism or carrying envelopes with an increased positive charge and fitness. In contrast, our previous data suggested that viruses carrying non-macrophage-tropic R5 envelopes were still predominant in immune tissue of AIDS patients. To further investigate the tropism and charge of HIV-1 viruses in late disease, we evaluated the properties of HIV-1 envelopes amplified from immune and brain tissues of AIDS patients with neurological complications. RESULTS: Almost all envelopes amplified were R5. There was clear compartmentalization of envelope sequences for four of the five subjects. However, strong compartmentalization of macrophage-tropism in brain was observed even when brain and immune tissue envelope sequences were not segregated. R5 envelopes from immune tissue of four subjects carried a higher
Non-mac-tropic HIV-1 R5 viruses are predominantly transmitted and persist in immune tissue even in AIDS patients who carry highly mac-tropic variants in the brain. Non-mac-tropic R5 envelopes (Envs) require high CD4 levels for infection contrasting with highly mac-tropic Envs, which interact more efficiently with CD4 and mediate infection of macrophages that express low CD4. Non-mac-tropic R5 Envs predominantly target T-cells during transmission and in immune tissue where they must outcompete mac-tropic variants. Here, we investigated whether Env+ pseudoviruses bearing transmitted/founder (T/F), early and late disease non-mac-tropic R5 envelopes mediated more efficient infection of CD4+ T-cells compared to those with highly mac-tropic Envs. Results: Highly mac-tropic Envs mediated highest infectivity for primary T-cells, Jurkat/CCR5 cells, myeloid dendritic cells, macrophages, and HeLa TZM-bl cells, although this was most dramatic on macrophages. Infection of primary T-cells mediated by all Envs ...
Aurigon is a research institute dedicated to preclinical services for human and veterinary pharmaceuticals, food and chemicals. It provides a full range of advisory and experimental services in pharmacology, bio-/analytics and toxicology.
Aurigon is a research institute dedicated to preclinical services for human and veterinary pharmaceuticals, food and chemicals. It provides a full range of advisory and experimental services in pharmacology, bio-/analytics and toxicology.
The A. salmonicida A450 LPS O-antigen, encoded by the wbsalmo gene cluster, is exported through an ABC-2 transporter-dependent pathway. It represents the first example of an O-antigen LPS polysaccharide with three different monosaccharides in their repeating unit assembled by this pathway. Until now, only repeating units with one or two different monosaccharides have been described. Functional genomic analysis of this wbsalmo region is mostly in agreement with the LPS O-antigen structure of acetylated l-rhamnose (Rha), d-glucose (Glc), and 2-amino-2-deoxy-d-mannose (ManN). Between genes of the wbsalmo we found the genes responsible for the biosynthesis and assembly of the S-layer (named A-layer in these strains). Through comparative genomic analysis and in-frame deletions of some of the genes, we concluded that all the A. salmonicida typical and atypical strains, other than A. salmonicida subsp. pectinolytica strains, shared the same wbsalmo and presence of A-layer. A. salmonicida subsp. pectinolytica
The Atlantic cod (Gadus morhua) is an important species for global fisheries and aquaculture industries. A thorough knowledge of the genes and molecular pathways involved in Atlantic cod immune responses will likely lead to the development of new diagnostics, vaccines, and other methods of combating infectious diseases that threaten these industries. Using functional genomic approaches, this research investigated the innate immune response in immune tissues (head kidney and spleen) of Atlantic cod following treatment with bacterial antigens (i.e. formalin-killed, atypical Aeromonas salmonicida, referred to as ASAL) or a viral mimic (i.e. polyriboinosinic polyribocytidylic acid, referred to as pIC). This research led to the identification of 4154 expressed sequence tags (ESTs) that were generated from cDNA libraries enriched for transcripts dysregulated following stimulation with ASAL. From these transcripts, 10 genes with immune-relevant functional annotations were selected for quantitative ...
An oxidase-negative Aeromonas salmonicida was isolated from coho salmon (Oncorhynchus kisutch) suffering from an epizootic of furunculosis at the state hatchery near Belfair, Washington. Typical, oxidase-positive A. salmonicida was isolated concurrently from the same population of fish. Mortality was controlled with medicated feed treatments. Evidence supporting the identification of the two types of A. salmonicida is presented. Methods for the proper identification of oxidase-negative A. salmonicida isolates are evaluated ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
A Inactivação fotodinâmica é um método simples e eficiente na inactivação de microorganismos. Inactivação fotodinâmica combina o uso de luz com um fotosensibilizador, como por exemplo porfirinas, que na presença de oxigénio gera a formação de espécies reactivas de oxigénio, como o oxigénio singleto e radicais livres, que são capazes de oxidar componentes membranares vitais. As principais vantagens da Terapia Fotodinâmica (TFD) são a sua eficiência na inactivação de bactérias, fungos, leveduras e protozoários; o baixo nível de indução de resistência; e o uso de fontes de luz baratas. Para melhor compreender esta técnica, é fundamental compreender o seu mecanismo de acções em alvos celulares. Os lípidos são importantes componentes nas membranas bacterianas, que muito recentemente foram reconhecidos como um dos alvos da PDI, e que podem estar envolvidos no processo de inactivação bacteriana. O principal objectivo deste estudo foi avaliar o efeito de quatro ...
Images of Furunculosis (Boil, Furuncle) Close Video. Etiology of Interdigital Furunculosis in Dogs The most common cause of interdigital furunculosis is a deep bacterial infection. Perianal fistula, which is also known as anal furunculosis, occurs when a dog experiences chronic lesions around their anus that worsen, leading to deep ulcers that drain and are painful to the dog. 12 Other bacteria or fungi occasionally cause furuncles or … This can cause inflammation and secondary bacterial infections. Furunculosis is a skin condition characterized by the formation of lump filled with pus. verify here. Lesions that recur despite therapy can also be a sign of an underlying disease, eg, atopy, hypothyroidism, or concurrent Malassezia infection. Abscesses in dogs are pockets of pus somewhere in the body, commonly including the skin, mouth, between the toes, or within the body cavity. Also see pet health content regarding abscesses between the toes in dogs. It has an acute onset and is highly ...
Defined deletion mutants demonstrate that the major secreted toxins are not essential for the virulence of Aeromonas salmonicida. - R Vipond, I R Bricknell, E Durant, T J Bowden, A E Ellis, M Smith, S MacIntyre
Aeromonas salmonicida was grown in a supplemented 3% (w/v) tryptone soya broth medium at 10 degrees C, a temperature at the lower end of the range over which furunculosis has been observed to occur in the field, and 25 ...
Dataset Title: Development of DNA primers to identify a Romet-resistance gene in Aeromonas salmonicida and its subsequent use for epidemiological studies ...
Cases reported • Furunculosis; Boils. On-line free medical diagnosis assistant. Ranked list of possible diseases from either several symptoms or a full patient history. A similarity measure between symptoms and diseases is provided.
Semantic Scholar extracted view of What is your diagnosis? Canine eosinophilic furunculosis (folliculitis) of the face. by Sven Van Poucke
Furunculosis answers are found in the 5-Minute Clinical Consult powered by Unbound Medicine. Available for iPhone, iPad, Android, and Web.
The Southern African Bursars of Independent Schools Association (SABISA) is a professional membership organisation for bursars and business managers at ISASA-affiliated schools.. ...
In this study, the immune reactions of rainbow trout (Oncorhynchus mykiss) were examined, after exposure to 10, 30 and 70% of tertiary-treated municipal sewage effluent for 27 days. Exposures were conducted concurrently with and without an immune challenge using intraperitoneal injections of inactivated Aeromonas salmonicida salmonicida. Due to the time required to prepare and analyse samples, fish sampling was conducted over two consecutive days. There was no trout mortality for any of the Show moreIn this study, the immune reactions of rainbow trout (Oncorhynchus mykiss) were examined, after exposure to 10, 30 and 70% of tertiary-treated municipal sewage effluent for 27 days. Exposures were conducted concurrently with and without an immune challenge using intraperitoneal injections of inactivated Aeromonas salmonicida salmonicida. Due to the time required to prepare and analyse samples, fish sampling was conducted over two consecutive days. There was no trout mortality for any of the ...
Definition of vibrio salmonicida in the Definitions.net dictionary. Meaning of vibrio salmonicida. What does vibrio salmonicida mean? Information and translations of vibrio salmonicida in the most comprehensive dictionary definitions resource on the web.
Behaviour of early life stages of the salmonid European grayling Thymallus thymallus was investigated by assessing the timing of larval downstream movement from spawning areas, the depth at which larvae moved and the distribution of juvenile fish during summer in two large connected river systems in Norway. Trapping of larvae moving downstream and electroshing surveys revealed that T. thymallus larvae emerging from the spawning gravel moved downstream predominantly during the night, despite light levels sufficient for orientation in the high-latitude study area. Larvae moved in the water mostly at the bottom layer close to the substratum, while drifting debris was caught in all layers of the water column. Few young-of-the-year still resided close to the spawning areas in autumn, suggesting large-scale movement (several km). Together, these observations show that there may be a deliberate, active component to downstream movement of T. thymallus during early life stages. This research ...
Affiliation:Faculty of Agriculture,Miyazaki University,農学部,教授, Research Field:General fisheries,Applied veterinary science, Keywords:魚類病原菌,Fish-pathogenic bacteria,Vibrio anguillarum,溶血素遺伝子,Aeromonas hydrophila,Pasteurella piscicida,Aeromonas salmonicida,魚類のグロビン遺伝子,溶血素,グロビンの塩基配列, # of Research Projects:7, # of Research Products:0
Aeromonas calmonicida is a Gram negative rod shaped bacterium capable of causing furunculosis in salmonid fish and other chronic and inflammatory diseases in goldfish, carp and also salmonids. The surface layer of A. salmonicida, the A-layer, has been demonstrated to be a major virulence factor for the organism , and its subunit A -protein has been purified and its Structural gene vapA has been cloned. The vapA gene from A. salmonicida strain A450 was subcloned (pSC150) and expressed in Escherichia coli. Its DNA sequence was then determined to consist of 1,506 bp encoding a 502-amino acid residue protein, containing a 21- residue signal peptide and a mature protein of 50,778 Dalton. The A-protein assembled on the cell surface in the form of an S-layer was refractile to trypsin cleavage while trypsin digestion of the purified mature protein revealed a highly resistant 39,400 Dalton N-terminal fragment and a 16,700 Dalton C-terminal fragment with moderate resistance, These trypsin-resistant ...
The story of kidnapping and torture victim Patrica Isasa (and her ultimate victory). Some background: The history of Argentina is full of change. Argentina is a very large country, and it became a strong economy after 1875. It received foreign investment, a lot of immigration from Europe, and it developed very effective modern agriculture. Argentina didnt have free elections until 1916. Then, Hipolito Yrigoyen won and became the first elected president. Like many of Argentinas presidents, Yrigoyen became unpopular for economic reasons. The Depression of 1929 resulted in a military coup in Argentina. After World War Two, free elections returned, and Juan Peron was elected president in 1946. His second wife, Eva Peron, was very popular. She became the subject of a famous American popular musical called Evita. However, she died in 1952. From 1948 to 1951, the peso lost value and inflation increased greatly. Once again, economic troubles in Argentina led to another military coup in 1955. This ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
NMR in biology. Learn. A2 Biology; Friday, 22 February 2013. Synergism between elevated temperature and nitrate: Impact on aerobic capacity of European grayling, Thymallusthymallus in warm, eutrophic waters. 1049-1054, 2011. The system can then be said to feed back into itself. Aquatic Conservation: Marine and Freshwater Ecosystems. Gracie Today, 10:45. Request PDF , The effect of network biology on drug toxicology , Introduction: The high failure rate of drug candidates due to toxicity, during clinical trials, is a critical issue in drug discovery. www.jeseh.net Effect of Cooperative Learning Strategy on Students Acquisition and Practice of Scientific Skills in Biology Hanadi Chatila, Fatima Al Husseiny Lebanese University To cite this article: Chatila, H. & Husseiny, F. (2017). UniProjects aim of providing this Effect Of Socio Economic Background On Academic Performance Of Secondary School Biology Students project … The filtrate becomes progressively more hyposmotic as it descends the loop. ...
Biology Assignment Help, The mirrows reach or grayling zone, The mirrows reach or grayling zone This zone has a less steep gradient, than the trout beck. The river here still flows swiftly, though the current is not fast and conditions are not torrential. Erosion is thus comparatively less. Some silt may
The collection of microbes that live in and on the human body is known as the microbiota. Bacteria live on the skin, in the nose and ears, and, most of all, in the gut. The role of the microbiome is so central to the bodys operations that it essentially acts as an organ . The microbiome impacts aging, digestion, the immune system, mood, and cognitive function. Your gut and your immune system are very closely linked, and 70 to 80 percent of your immune tissue is situated in your digestive tract. ...
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Bacteriophages are an important repository of genetic diversity. As one of the major constituents of terrestrial biomass, they exert profound effects on the earths ecology and microbial evolution by mediating horizontal gene transfer between bacteria and controlling their growth. Only limited genomic sequence data are currently available for phages but even this reveals an overwhelming diversity in their gene sequences and genomes. The contribution of the T4-like phages to this overall phage diversity is difficult to assess, since only a few examples of complete genome sequence exist for these phages. Our analysis of five T4-like genomes represents half of the known T4-like genomes in GenBank. Here, we have examined in detail the genetic diversity of the genomes of five relatives of bacteriophage T4: the Escherichia coli phages RB43, RB49 and RB69, the Aeromonas salmonicida phage 44RR2.8t (or 44RR) and the Aeromonas hydrophila phage Aeh1. Our data define a core set of conserved genes common to these
Aeromonas hydrophila. Coloured transmission electron micrograph of thin-sectioned cells of Aeromonas hydrophila bacteria. It is a Gram- negative, rod-shaped bacillus. Aeromonas hydrophila is associated with septicaemia, pneumonia and gastroenteritis in humans. In particular it causes opportunistic infections in patients with lowered immunity, such as in cancer and liver disease. Aeromonas hydrophila has been isolated from urine, sputum, faeces and bile. It is resistant to many types of antibiotic including penicillin, but can be controlled with tetracyclins and gentamicin. Magnification: x28,800 at 6x4.5cm size. - Stock Image B220/0809
Furunculosis Multidisciplinary Fish Disease Research by Eva-Maria Bernoth http://i.imgur.com/raL2R3J.jpg Pages: 529 Publisher: -- Edition: 1997 Language: English ISBN: 9780120930401
Aeromonas hydrophila ATCC ® 7966D-5™ Designation: Genomic DNA from Aeromonas hydrophila TypeStrain=True Application: Water testing
Infeksi bakteri merupakan salah satu masalah yang serius dalam pemeliharaan ikan, karena itu diagnosa yang dilakukan terhadap penyakit bakterial harus dilakukan dengan setepat mungkin. Selama bertahun-tahun banyak bakteri yang sudah dapat diidentifikasi sebagai penyebab sakit pada ikan salah satunya Aeromonas (Dixon, 1990). Aeromonas terdapat di air tawar, tanah dan pada ikan (Post, 1987). Merupakan bakteri…
Biohazard level, growth media and temperature, gram stain, industrial applications and more information for Aeromonas ichthiosmia.
Aeromonas dhakensis was first isolated from children with diarrhea in Dhaka, Bangladesh and described in 2002. In the past decade… Expand ...
While HIV cell-to-cell transmission has been observed in test tube experiments, this is the first study to capture these interactions in a living animal. Although cell-to-cell infection does result in release of abundant solo viral particles, direct transmission from HIV-infected immune cells to other cells-which can then replicate in clusters of these cells-is a much more efficient route to quickly spread the virus, researchers say. It may be particularly important in allowing the virus to spread in the body even before it is detectable in the blood.. Previous studies in cell culture have indicated that cell-to-cell infection may help HIV to resist antibodies and potent therapies. This study provides direct evidence that these interactions do occur in infected immune tissues, and highlight the importance of considering cell-to-cell transmission in developing new treatments.. All HIV treatment to date has been based on the free-floating virion model, says Benjamin K. Chen, MD, PhD, an ...
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Scottish Government (February 2018). "Aeromonas Salmonicida". Retrieved 31 March 2020. Responsible Use of Medicines in ... Aeromonas salmonicida) in 1994, which significantly reduced the need for antibiotic treatments, but the trout sector is still ...
Bacteria which give negative results for the indole test include: Actinobacillus spp., Aeromonas salmonicida, Alcaligenes sp., ... Bacteria that test positive for cleaving indole from tryptophan include: Aeromonas hydrophila, Aeromonas punctata, Bacillus ...
"Haemorrhagic septicaemia by Aeromonas salmonicida subsp. salmonicida in a black-tip reef shark (Carcharhinus melanopterus)". ... caused by the bacterium Aeromonas salmonicida subsp. salmonicida. As often the most abundant apex predator within its ecosystem ...
R.C. Cipriano; G.L. Bullock (2001). Furunculosis And Other Diseases Caused By Aeromonas salmonicida (PDF) (Report). Fish ...
Research suggests two pathogens are responsible for the disease, Listonella anguillarum and Aeromonas salmonicida. Infection ...
Aeromonas salmonicida, a Gram-negative bacteria, causes the disease furunculosis in marine and freshwater fish. Streptococcus ...
Aeromonas salmonicida, a Gram-negative bacteria, causes the disease furunculosis in marine and freshwater fish. Streptococcus ... "Furunculosis And Other Diseases Caused By Aeromonas salmonicida" Archived 2009-05-07 at the Wayback Machine Fish Disease ...
This group includes the phages Aeromonas salmonicida phage 56, Vibrio cholerae phages 138 and CP-T1, Bdellovibrio phage φ1422 ... Iodobacter phage phiPLPE Species Aeromonas phage 56 Aggregatibacter phage Aaphi23 Bdellovibrio phage phi1402 Bdellovibrio phage ...
Immobilized Aeromonas salmonicida ATCC 27013, when exposed to thymidine and 5-fluorouracil in phosphate buffer at room ...
However, unlike many other Aeromonas species such as A. hydrophilia and A. punctata, A. salmonicida is indole negative, which ... including Aeromonas salmonicida (mistaken by ID32 GN) and Sphingobacterium spp. (mistaken by Vitek 2), may be confused with ...
Species known to enter a VBNC state: E.M.S Aeromonas salmonicida Agrobacterium tumefaciens Burkholderia cepacia Burkholderia ...
Aeromonas salmonicida, Helicobacter pylori, Vibrio cholerae and Yersinia pestis. Thus, LysE family members are found widely ...
Aeromonas MeSH B03.440.450.019.025.380 - Aeromonas hydrophila MeSH B03.440.450.019.025.690 - Aeromonas salmonicida MeSH B03.440 ... Aeromonas MeSH B03.660.250.017.025.380 - Aeromonas hydrophila MeSH B03.660.250.017.025.690 - Aeromonas salmonicida MeSH B03.660 ... Vibrio salmonicida MeSH B03.440.450.900.859.900 - Vibrio vulnificus MeSH B03.440.450.980 - Zymomonas MeSH B03.440.475.100 - ... Vibrio salmonicida MeSH B03.660.250.830.830.900 - Vibrio vulnificus MeSH B03.660.250.915 - xanthomonadaceae MeSH B03.660. ...
Elizabethkingia meningoseptica may be mistaken for Aeromonas salmonicida. There is literature on this subject, but many papers ... Aeromonas aides leeches in digesting blood meals. H. medicinalis used after surgery has led to Aeromonas infections, most ... Aeromonas pneumonia due to episodes of near-drowning are frequently complicated by bacteremia and death. Aeromonas species are ... Members of the genus Aeromonas can be referred to as aeromonads (viz. trivialisation of names). Parte, A. C. "Aeromonas". LPSN ...
This toxin has been characterized in A. hydrophila (human diarrhoeal isolate), A. salmonicida (fish pathogen), and A. jandaei ... Aeromonas hydrophila Oregon State University. Arrow Scientific. Aeromonas hydrophila "Aeromonas hydrophila." Bad Bug Book ... "Georgia woman with flesh-eating disease leaves hospital" Aeromonas hydrophila ATCC7966 Type strain of Aeromonas hydrophila at ... Prevalence and distribution of Aeromonas hydrophila in the United States Clinical and microbiological features of Aeromonas ...
Type strain of Aeromonas salmonicida subsp. smithia at BacDive - the Bacterial Diversity Metadatabase Type strain of Aeromonas ... salmonicida at BacDive - the Bacterial Diversity Metadatabase Type strain of Aeromonas salmonicida subsp. achromogenes at ... Rose, A; Ellis, E (1990). "The survival of Aeromonas salmonicida subsp. salmonicida in sea water". The Journal of Fish Disease ... "The genome of Aeromonas salmonicida subsp. salmonicida A449: insights into the evolution of a fish pathogen". BMC Genomics. 9: ...
Aeromonas salmonicida. *Cyanobacteria. *Cyanobiont. *Cyanotoxin. *Enteric redmouth disease. *Flavobacterium. *Flavobacterium ...
Aeromonas salmonicida. *Cyanobacteria. *Cyanobiont. *Cyanotoxin. *Enteric redmouth disease. *Flavobacterium. *Flavobacterium ...
Aeromonas salmonicida. *Cyanobacteria. *Cyanobiont. *Cyanotoxin. *Enteric redmouth disease. *Flavobacterium. *Flavobacterium ...
Aeromonas salmonicida. *Columnaris. *Enteric redmouth. *Fin rot. *Fish dropsy. *Flavobacterium. *Hematopoietic necrosis ...
Aeromonas salmonicida. *Cyanobacteria. *Cyanobiont. *Cyanotoxin. *Enteric redmouth disease. *Flavobacterium. *Flavobacterium ...
Aeromonas salmonicida. *Columnaris. *Enteric redmouth. *Fin rot. *Fish dropsy. *Flavobacterium. *Hematopoietic necrosis ...
Aeromonas salmonicida. *Cyanobacteria. *Cyanobiont. *Cyanotoxin. *Enteric redmouth disease. *Flavobacterium. *Flavobacterium ...
nov., Aliivibrio salmonicida comb. nov. and Aliivibrio wodanis comb. nov". International Journal of Systematic and Evolutionary ... Genetic studies have shown the other two original members-Aeromonas and Plesiomonas-belong to separate families. The family ... Vibrio salmonicida and Vibrio wodanis as Aliivibrio fischeri gen. nov., comb. nov., Aliivibrio logei comb. ...
Development of Standardized Antimicrobial Susceptibility Testing Methods and Aeromonas salmonicida Epidemiologic Cutoff Values ... against Aeromonas salmonicida, causative agent of furunculosis in salmonids. To achieve this goal we first established a ... salmonicida-challenged fish. We confirmed OTC to be highly efficacious against a susceptible A. salmonicida strain in vivo, and ... salmonicida isolates (1 μg/mL) yields a product greater than this AUCss/MIC target of ≥5. This work demonstrates PK/PD indices ...
First isolation of Vibrio tapetis and an atypical strain of Aeromonas salmonicida from skin ulcerations in common dab (Limanda ...
The small subunit ribosomal RNA (SSU rRNA) encoding genes from reference strains of Aeromonas salmonicida subsp, smithia and ... Thornton, JM (1)Subjectaeromonas salmonicida (1)haemophilus piscium (1) ribosomal rna genes (1) ... Small subunit rrna gene sequences of aeromonas salmonicida subsp. smithia and haemophilus piscium reveal pronounced ... salmonicida Thornton, JM; Austin, DA; Austin, B; Powell, R (Inter-Research Science Center, 1999-01-01) ...
Aeromonas salmonicida * Scanning Transmission Electron Microscopy * Type III secretion translocation pores of Yersinia ...
... an Endogenous Strain from Brook Charr with Antagonistic Properties Against Aeromonas salmonicida subsp. salmonicida. Genome ... The etiological agent, Aeromonas salmonicida, is naturally present in the resident microbial community of salmonids. ... Fig I. TEM micrograph of Aeromonas salmonicida, the causative agent of salmonid furunculosis,. ... and Two Endogenous Probiotics That Inhibit Aeromonas salmonicida In Vitro. Microorganisms. 2019 Jul 6;7(7). pii: E193. ...
Role of surface components catholic senior singles online dating site in serum resistance of virulent aeromonas salmonicida. ...
... with aeromonas salmonicida subsp. Alcamim guesthouse åpnes i sextips for henne fettkul på magen et nytt vindu. Romerske menn ...
Transcriptional changes in innate immunity genes in head kidneys from Aeromonas salmonicida-challenged rainbow trout fed a ...
Transcriptional changes in innate immunity genes in head kidneys from Aeromonas salmonicida-challenged rainbow trout fed a ...
Transcriptional changes in innate immunity genes in head kidneys from Aeromonas salmonicida-challenged rainbow trout fed a ...
Transcriptional changes in innate immunity genes in head kidneys from Aeromonas salmonicida-challenged rainbow trout fed a ...
Transcriptional changes in innate immunity genes in head kidneys from Aeromonas salmonicida-challenged rainbow trout fed a ...
Transcriptional changes in innate immunity genes in head kidneys from Aeromonas salmonicida-challenged rainbow trout fed a ...
Transcriptional changes in innate immunity genes in head kidneys from Aeromonas salmonicida-challenged rainbow trout fed a ...
Transcriptional changes in innate immunity genes in head kidneys from Aeromonas salmonicida-challenged rainbow trout fed a ...
Transcriptional changes in innate immunity genes in head kidneys from Aeromonas salmonicida-challenged rainbow trout fed a ...
Transcriptional changes in innate immunity genes in head kidneys from Aeromonas salmonicida-challenged rainbow trout fed a ...
Transcriptional changes in innate immunity genes in head kidneys from Aeromonas salmonicida-challenged rainbow trout fed a ...
Aeromonas popoffii * "Aeromonas proteolytica" * Aeromonas rivipollensis * Aeromonas rivuli * Aeromonas salmonicida * Aeromonas ... Parent taxon: Aeromonas Stanier 1943 (Approved Lists 1980) Assigned by: Snieszko SF. Genus IV. Aeromonas Kluyver and van Niel ... Aeromonas punctata is the correct name if this species is regarded as a separate species within a separate genus Aeromonas. ... Aeromonas punctata (Zimmermann 1890) Snieszko 1957 (Approved Lists 1980), Aeromonas punctata subsp. caviae (Scherago 1936) ...
Aeromonas salmonicida * Cyanobacteria * Cyanobiont * Cyanotoxin * Enteric redmouth disease * Flavobacterium * Flavobacterium ...
Aeromonas caviae Ae398 (incomplete) *Aeromonas hydrophila subsp. hydrophila ATCC 7966 (complete) *Aeromonas salmonicida subsp. ... Aliivibrio salmonicida LFI1238 (complete) *Alkalilimnicola ehrlichii MLHE-1 (complete) *Allochromatium vinosum DSM 180 ( ... salmonicida A449 (complete) *Aggregatibacter actinomycetemcomitans D11S-1 (complete) *Aggregatibacter actinomycetemcomitans D7S ...
Jeffs Ph. D. will allow research and development of endogenous probiotic-based therapeutic tools against opportunistic infections in salmonid aquaculture. ...
  • Aeromonas salmonicida subsp. (atcc.org)
  • PstI differentiated all the atypical A. salmonicida from A. salmonicida subsp. (nih.gov)
  • Effective qPCR methodology to quantify the expression of virulence genes in Aeromonas salmonicida subsp. (nih.gov)
  • This study aimed to select and validate different methodological strategies to quantify the expression of the virulence genes ascC and ascV by qPCR in Aeromonas salmonicida subsp. (nih.gov)
  • Through comparative genomic analysis and in-frame deletions of some of the genes, we concluded that all the A. salmonicida typical and atypical strains, other than A. salmonicida subsp. (mdpi.com)
  • A. salmonicida subsp. (mdpi.com)
  • Intraperitoneal injection of a lethal dose of Aeromonas salmonicida subsp. (hindawi.com)
  • Molecular Characterization and Quantitative Analysis of Superoxide Dismutases in Virulent and Avirulent Strains of Aeromonas salmonicida subsp. (asm.org)
  • Levels of SOD were significantly higher in virulent than in avirulent strains of A . salmonicida subsp. (asm.org)
  • Prophylactic measures against Aeromonas salmonicida subsp. (ovid.com)
  • These were compared to 11 Swedish, 1 Icelandic aAS isolates, to 3 A. salmonicida subsp. (jyu.fi)
  • salmonici da (ASS) isolates and to ATCC 33659, a type strain for A. salmonicida subsp. (jyu.fi)
  • The genome of Aeromonas salmonicida subsp. (biomedcentral.com)
  • Potential role of specific antibodies in vaccine-induced protection against Aeromonas salmonicida subsp. (dtu.dk)
  • Furunculosis caused by infection with Aeromonas salmonicida subsp. (dtu.dk)
  • In this study we have examined the levels of protection against infection with a Danish strain of A. salmonicida subsp. (dtu.dk)
  • Structure determination of the virulence factor AsaP1 from Aeromonas salmonicida subsp. (technologynetworks.com)
  • Characterization and diversity of phages infecting Aeromonas salmonicida subsp. (nature.com)
  • Aeromonas hydrophila subsp. (ebi.ac.uk)
  • CCEB 705 Aeromonas salmonicida subsp. (straininfo.net)
  • Crystallization and Preliminary X-Ray Diffraction Studies of Asap1_E294A and Asap1_E294Q, Two Inactive Mutants of the Toxic Zinc Metallopeptidase Asap1 from Aeromonas Salmonicida Subsp. (rcsb.org)
  • The Asap1 Peptidase of Aeromonas Salmonicida Subsp. (rcsb.org)
  • 1H-NMR (nuclear magnetic resonance)-based chemometric methods have been applied for the first time to investigate changes in the plasma metabolite profiles of Atlantic salmon Salmo salar as a result of exposure to Aeromonas salmonicida subsp. (canada.ca)
  • The extract obtained in late spring showed strong antibacterial activity against Aeromonas salmonicida subsp. (frontiersin.org)
  • salmonicida, Vibrio alginolyticus , and V. vulnificus , and moderate activity against Photobacterium damselae subsp. (frontiersin.org)
  • Susceptibility of Atlantic cod Gadus morhua, halibut Hippoglossus hippoglossus and wrasse (Labridae) to Aeromonas salmonicida subsp. (bibsys.no)
  • Experimental infection of turbot Scophthalmus maximus and halibut Hippoglossus hippoglossus yolk sac larvae with Aeromonas salmonicida subsp. (bibsys.no)
  • The susceptibility of early life stages of turbot Scophthalmus maximus and halibut Hippoglossus hippoglossus to Aeromonas salmonicida subsp. (bibsys.no)
  • Inaktivert Aeromonas salmonicida subsp. (felleskatalogen.no)
  • Atlantisk laks (Salmo salar L.): Aktiv immunisering for å redusere kliniske symptomer og dødelighet forårsaket av infeksjoner med Aeromonas salmonicida subsp. (felleskatalogen.no)
  • Salmon Trout Cyprinids Pike Perch Bullheads Turbot Halibut A. salmonicida, an airborne pathogen, can travel 104 cm from its host into the atmosphere and back to the water, thus making it difficult to control. (wikipedia.org)
  • The S-layer of the fish pathogen Aeromonas salmonicida , the causative agent of furunculosis in salmonids, is probably the best characterized S-layer in regards to function. (springer.com)
  • salmonicida interacts with its host and the environment, and therefore to the prevention of epizootics due to this pathogen. (nih.gov)
  • The recruitment of cellular factors of innate immunity rather than induced expression of pathogen receptors is hence of key importance for mounting a first immune defense against invading A. salmonicida . (hindawi.com)
  • This first report of defined deletion mutations within any proposed extracellular virulence factor of A. salmonicida raises crucial questions about the pathogenesis of this important fish pathogen. (curehunter.com)
  • An aberrant strain of the bacterial fish pathogen Aeromonas salmonicida isolated from a marine host, the sablefish ( Anaplopoma fimbria ), and from two species of cultured Pacific Salmon. (microbiologyresearch.org)
  • Interaction of the fish pathogen Aeromonas salmonicida with ra. (mysciencework.com)
  • Immunostimulants were given to rainbow trout for assaying effects on modulating non-specific defense mechanisms, specific immune response, and protection levels against pathogen challenge with Aeromonas salmonicida. (elsevier.com)
  • Challenge tests with the virulent pathogen, A. salmonicida, showed a 5-6 day delay in the onset of mortalities in the fish given the immunostimulants alone, and a 12-14 day delay when immunostimulants given were combined with the bacterin. (elsevier.com)
  • The Gram-negative bacterium Aeromonas salmonicida is a fish pathogen for various fish species worldwide. (rcsb.org)
  • This study suggests that with further development this metabolite profiling technique may be a useful tool for diagnosis of disease states in salmon and could provide a better understanding of the host-pathogen relationship which at present is poorly understood for A. salmonicida and Atlantic salmon. (canada.ca)
  • The Gram negative bacterial fish pathogen Aeromonas salmonicida is the causative agent of furunculosis in salmonid fishes. (mun.ca)
  • Aeromonas salmonicida is an important fish pathogen that produces a wide and varied array of virulence factors. (biomedcentral.com)
  • Class 1 integrons were found in 26 of 40 antibiotic-resistant isolates of the fish pathogen Aeromonas salmonicida from Northern Europe and North America. (qxmd.com)
  • . A. salmonicida has been recognized as a pathogen of fish for over 100 years (Abbott et al. (ispub.com)
  • While A. salmonicida was traditionally thought of as a pathogen of salmonids, global reports now confirm that this pathogen has been associated with clinical or covert disease in a variety of salmonid and non-salmonid species in freshwater, brackish water and sea water. (ispub.com)
  • 23 °C. While co-infection of smallmouth bass by both A. salmonicida and largemouth bass virus can be devastating to juvenile smallmouth bass, the optimal temperatures of each pathogen are 7-10 °C apart, making their synergistic effects highly unlikely under field conditions. (biomedcentral.com)
  • salmonicida strain was evaluated by three quantification models, including single (least or most stable) or three most stable reference genes, combined with constant or specific gene amplification efficiency. (nih.gov)
  • salmonicida strain A449 when cultured in vitro and in vivo. (asm.org)
  • We confirmed OTC to be highly efficacious against a susceptible A. salmonicida strain in vivo, and through pharmacokinetics studies, calculated the OTC AUCss in healthy and challenged fish to be 27.2 and 20.1 μg∙h/mL, respectively. (umd.edu)
  • Resistance to ampicllin was induced experimentally in A. salmonicida CECT894T (AsWT), creating a derivate ampicillin resistant strain (AsR). (ua.pt)
  • This loss may have occurred for the type strain of A. salmonicida ssp. (biomedcentral.com)
  • The biochemical activities of the isolated strains were identical to the activities of the standard strain of A. salmonicida , which served as a control. (ispub.com)
  • salmonicida is a facultatively intracellular gram-negative bacterium that is the etiological agent of furunculosis, a bacterial septicemia of salmonids that causes significant economic loss to the salmon farming industry. (asm.org)
  • salmonicida, the causative agent of furunculosis, have been an active field of research for decades, with studies mainly focused on Atlantic salmon (Salmo salar). (ovid.com)
  • The goal of this work was to determine the relevant PK/PD index target (AUCss/MIC) for oxytetracycline (OTC) against Aeromonas salmonicida, causative agent of furunculosis in salmonids. (umd.edu)
  • A comprehensive bacteriophage typing scheme for Aeromonas salmonicida , the causal agent of furunculosis in fish, is described. (microbiologyresearch.org)
  • salmonicida, a Gram-negative bacterium that is the etiological agent of furunculosis. (canada.ca)
  • A. salmonicida isolates flourish when grown on blood agar or tyrosine. (wikipedia.org)
  • To achieve this goal we first established a standardized MIC testing method for aquatic bacterial pathogens, then used this method to determine the in vitro susceptibility cutoff concentration (epidemiologic cutoff value) for OTC (and three other antimicrobial agents) against 217 A. salmonicida isolates. (umd.edu)
  • Either of the AUCss values divided by the current epidemiologic cutoff value for A. salmonicida isolates (1 μg/mL) yields a product greater than this AUCss/MIC target of ≥5. (umd.edu)
  • The plasmid profiles of oxytetracycline- and streptomycin-resistant isolates of Aeromonas salmonicida, Vibrio anguillarum, and Vibrio ordalii were examined by agarose gel electrophoresis. (islandscholar.ca)
  • were predominantly plasmidless, except for a 47-kilobase (kb) Show more The plasmid profiles of oxytetracycline- and streptomycin-resistant isolates of Aeromonas salmonicida, Vibrio anguillarum, and Vibrio ordalii were examined by agarose gel electrophoresis. (islandscholar.ca)
  • Atlantic coast isolates of A. salmonicida possessed four or six plasmids, with four smaller plasmids ranging in size from 4.3 to 8.1 kb being consistently observed. (islandscholar.ca)
  • The plasmid profiles of the Pacific coast isolates of A. salmonicida varied slightly from those of the Atlantic coast isolates with six plasmids observed, ranging in size from 4.2 to 8.9 kb. (islandscholar.ca)
  • In the present study, we compile A-layer typing results from a total of 675 A. salmonicida isolates, recovered over a 59-year period from 50 different fish species in 26 countries. (wur.nl)
  • Here we used iron deprivation by addition of the chelator 2'2-dipyridyl to induce the expression of several such virulence factors in three isolates of Aeromonas salmonicida (one avirulent and two virulent). (biomedcentral.com)
  • Methods for the proper identification of oxidase-negative A. salmonicida isolates are evaluated. (cefas.co.uk)
  • salmonicida was provided by the recovery of eight atypical isolates from ulcers (but not from kidney tissue) on goldfish, carp and roach. (coursehero.com)
  • Atypical Aeromonas salmonicida vapA type V and Vibrio spp. (stir.ac.uk)
  • Papadopoulou A, Wallis T, Ramirez-Paredes JG, Monaghan SJ, Davie A, Migaud H & Adams A (2020) Atypical Aeromonas salmonicida vapA type V and Vibrio spp. (stir.ac.uk)
  • Contains formalin inactivated cultures of Aeromonas salmonicida , Vibrio anguillarum serotypes I & II, Vibrio ordalii and Vibrio salmonicida serotypes I & II in liquid emulsion with an oil based adjuvant. (drugs.com)
  • Vibrio anguillarum , V. ordalii and Aliivibrio salmonicida . (cabi.org)
  • Antibodies against Vibrio salmonicida lipopolysaccharide (LPS) and whole bacteria in sera from Atlantic salmon ( Salmo salar L.) vaccinated during the smolting and early post smolt period. (uib.no)
  • Typically, Aeromonas hydrophilia, Pseudomonas and Vibrio . (fishdoc.co.uk)
  • Salmonicida RPS ≥70, Vibrio anguillarum serovar O1 og O2α, RPS 60 ≥75, Vibrio salmonicida RPS 60 ≥90, Moritella viscosa RPS ≥70, formalininaktivert infeksiøs pankreasnekrosevirus (IPNV) dyrket på cellekultur (≥1 ELISA-enheter), emulgatorer. (felleskatalogen.no)
  • Stimulerer aktiv immunitet mot Aeromonas salmonicida, Vibrio anguillarum serovar O1 og O2α, Vibrio salmonicida, Moritella viscosa og IPNV. (felleskatalogen.no)
  • salmonicida (furunkulose), Vibrio anguillarum serovar O1 og O2α (vibriose), Vibrio salmonicida (kaldtvannsvibriose), IPNV (infeksiøs pankreasnekrosevirus) og Moritella viscosa (vintersår). (felleskatalogen.no)
  • Southern analyses showed that both ISAS1 and ISAS2 were restricted to A. salmonicida strains A449 and A450 where they were present in low copy number. (nih.gov)
  • ABSTRACT: The survival of 'atypical', cytochrome oxidase-negative Aeromonas salmonicida in microcosms containing sterilized water and sediment was assessed using the plate count method. (int-res.com)
  • In vitro susceptibilities of 30 Aeromonas salmonicida strains isolated from rainbow trout ( Oncorhynchus mykiss ) were determined against to 23 antimicrobials by using disc diffusion method. (scialert.net)
  • Anderson, DP & Jeney, G 1992, ' Immunostimulants added to injected Aeromonas salmonicida bacterin enhance the defense mechanisms and protection in rainbow trout (Oncorhynchus mykiss) ', Veterinary Immunology and Immunopathology , vol. 34, no. 3-4, pp. 379-389. (elsevier.com)
  • The lipopolysaccharide (LPS) from A. salmonicida, a major factor in determining bacterial virulence, was incorporated into liposomes to determine the ability of these formulations to function as delivery vehicles and immunological adjuvants in rainbow trout (Oncorhynchus mykiss). (mun.ca)
  • The aim was to determine if the bacteria Aeromonas salmonicida was the cause of the disease furunculosis, in Californian trout ( Oncorhynchus mykiss , Walbaum 1792), as well as certain biochemical activities of the isolated strains. (ispub.com)
  • Isolation and phenotypic characterization of an oxidase-negative Aeromonas salmonicida causing furunculosis in coho salmon (Oncorhynchus kisutch). (cefas.co.uk)
  • An oxidase-negative Aeromonas salmonicida was isolated from coho salmon (Oncorhynchus kisutch) suffering from an epizootic of furunculosis at the state hatchery near Belfair, Washington. (cefas.co.uk)
  • Contribution of food deprivation to the immune response in rainbow trout ( Oncorhynchus mykiss ) vaccinated against Cryptobia salmositica and Aeromonas salmonicida . (uoguelph.ca)
  • Effect of water temperature on the growth of Aeromonas salmonicida inoculated into yamame, Oncorhynchus masou. (cefas.co.uk)
  • The type species is Aeromonas hydrophila . (thefreedictionary.com)
  • Species Aeromonas salmonicida and A. hydrophila with related species A. bestiarum are included in the so-called 'A. hydrophila' complex (Janda and Abott, 1998). (ispub.com)
  • Aeromonas salmonicida and A. hydrophila . (cabi.org)
  • The supplementation of AiiA AI96 into fish feed by oral administration significantly attenuated Aeromonas hydrophila infection in zebrafish. (asm.org)
  • Aeromonas salmonicida's ability to infect a variety of hosts, multiply, and adapt, make it a prime virulent bacterium. (wikipedia.org)
  • The disease is caused by Aeromonas salmonicida, a Gram nagative bacterium that is highly virulent and is readily transmitted horozontally. (nasa.gov)
  • The tetragonal paracrystalline surface protein array (A-layer) of the fish pathogenic bacterium Aeromonas salmonicida is a virulence factor and bacteria which are unable to produce A-layer are attenuated in their ability to kill fish. (nih.gov)
  • Aeromonas salmonicida are specific bacteria that can cause infections and death to the cultivation of carp (Cyprinus carpio ) during larval stage. (unja.ac.id)
  • In tendency of determination the presence of this bacteria in trout it has been done the rapid agglutination with the "O" antiserum against A. salmonicida which was a positive reaction. (ispub.com)
  • Several fish-pathogenic bacteria and parasites were reported from fish collected during the course of mortality episode examinations, such as Aeromonas spp. (biomedcentral.com)
  • AF218037 Aeromonas salmonicida RTX protein (asx) gene, complete cds. (atcc.org)
  • Mutagenesis of the paracrystalline surface protein array of Aeromonas salmonicida by endogenous insertion elements. (nih.gov)
  • Ten independent mutants of Aeromonas salmonicida which were unable to produce A-layer were isolated by growth at 30 degrees C. These mutants displayed either reduced synthesis of the A-layer subunit, synthesis of a truncated subunit, or complete loss of the ability to produce the subunit protein. (nih.gov)
  • The ability of typical and atypical strains of Aeromonas salmonicida to utilize non-haem sources of protein-bound iron was evaluated. (microbiologyresearch.org)
  • These results indicate that typical and atypical strains of A. salmonicida differ in their mechanism of utilization of non-haem protein-bound sources of iron. (microbiologyresearch.org)
  • An intact crystalline protein array, the A-layer, mediated adherence of A. salmonicida cells to M phi even in the absence of opsonins. (mysciencework.com)
  • A recently described typing system based on sequence variation in the virulence array protein (vapA) gene, encoding the A-layer surface protein array, allows unambiguous subtyping of Aeromonas salmonicida. (wur.nl)
  • Protein expression and transcription profiles of three strains of Aeromonas salmonicida ssp. (biomedcentral.com)
  • Our collective findings provide new insights into protein phosphorylation patterns, which may serve as effective indicators of A. salmonicida infection in Atlantic salmon. (fis.com)
  • The A-layer protein of Aeromonas salmonicida : further characterization and a new isolation procedure. (nii.ac.jp)
  • The extracellular products of A. salmonicida consist of 25 proteins, enzymes, and toxins, and many more. (wikipedia.org)
  • In a previous study, we identified proteins that are significantly upregulated in kidneys of Atlantic salmon challenged with A. salmonicida. (fis.com)
  • To our knowledge, this is the first study to characterize phosphorylation events in proteins from A. salmonicida-infected Atlantic salmon. (fis.com)
  • Aeromonas salmonicida is a pathogenic bacterium that severely impacts salmonid populations and other species. (wikipedia.org)
  • Aeromonas salmonicida (A. salmonicida) is a pathogenic bacterium that causes furunculosis and poses a significant global risk, particularly in economic activities such as Atlantic salmon (Salmo salar) farming. (fis.com)
  • Aeromonas salmonicida is a Gram-negative, facultatively anaerobic, nonmotile bacterium. (wikipedia.org)
  • Using this method and a variety of well-characterized Aeromonas salmonicida strains with normal or altered cell surfaces, we investigated the role of this unusual bacterial surface in the bacterium-M phi interaction. (mysciencework.com)
  • Aeromonas salmonicida is a bacterium that includes Quarantine Fish Disease and has spread on the island of Java. (journalcra.com)
  • The main goal of this study was to evaluate the charge effect of four porphyrin derivatives used as photosensitizers in PDI in the photo-oxidation of membrane lipids of Aeromonas salmonicida and relate with the inactivation of this bacterium. (ua.pt)
  • X74681 A.salmonicida salmonicida (ATCC 33658T) gene for 16S ribosomal RNA. (atcc.org)
  • Ten strains of the species A. salmonicida were isolated from samples taken from changed skin areas on fish raised in fisheries and all were immobile. (ispub.com)
  • Aeromonas salmonicida is a gram negative, immobile, rod-shaped species of Aeromonodicae, size 1.3 - 2.0 by 0.8 - 1.3 µm that grows at the temperature of 22-23°C, but never at 37°C (Inglis et al. (ispub.com)
  • In addition to trout, A. salmonicida is present in some other fish species, such as, carp ( Cyprinus carpio , L. 1758), perch ( Perca fluviatilis, L. 1758), tench ( Tinca tinca , L. 1758) and pike ( Esox lucius , L. 1758). (ispub.com)
  • A species-specific DNA fragment was cloned from Aeromonas salmonicida by RAPD-PCR.A set of PCR primers was developed for the detection of the speciesspecific DNA region from fish tissues which were infected with A.salmonicida. (nii.ac.jp)
  • U68075 Aeromonas salmonicida coded portion of proteolysis tag tmRNA gene. (atcc.org)
  • The A. salmonicida A450 LPS O-antigen, encoded by the wb salmo gene cluster, is exported through an ABC-2 transporter-dependent pathway. (mdpi.com)
  • Cloning and study of the genetic organization of the exe gene cluster of Aeromonas salmonicida. (kingston.ac.uk)
  • Karlyshev, A V and MacIntyre, S (1995) Cloning and study of the genetic organization of the exe gene cluster of Aeromonas salmonicida. (kingston.ac.uk)
  • Rainbow trout have been found to carry A. salmonicida up to two years after initial infection without re-exposure. (wikipedia.org)
  • We conducted additional in vivo studies using rainbow trout to monitor achievable serum OTC concentrations in both healthy and A. salmonicida-challenged fish. (umd.edu)
  • salmonicida in both non-vaccinated, as well as vaccinated rainbow trout. (dtu.dk)
  • Immunization experiments demonstrated that multi- and unilamellar vesicles composed of both positively and negatively charged lipid are capable of prolonging the humoral immune response to LPS from A. salmonicida in rainbow trout when compared to the non-incorporated LPS. (mun.ca)
  • First described in the 19th century, Aeromonas salmonicida is considered today as one of the oldest known fish pathogens. (biomedcentral.com)
  • Thus, although these two toxins may confer some competitive advantage to A. salmonicida, neither toxin is essential for the very high virulence of A. salmonicida in Atlantic salmon. (curehunter.com)
  • Effects of immunization with T-dependent and T-independent Aeromonas salmonicida antigens on antibody production and immunological memory in cultured Atlantic salmon, Salmo salar L. (canada.ca)
  • Combined ribopatterns ClaI/PstI divided pigment producing atypical strains into four major groups B/B, G/G, G/T, F/F. Most of the achromogenic oxidase-positive strains belonged to two major groups H/H or U/U. Cluster analysis of ribopatterns and plasmid profile analysis also supported the division of atypical oxidase-positive A. salmonicida into pigment-producing and achromogenic groups. (nih.gov)
  • Defined deletion mutants demonstrate that the major secreted toxins are not essential for the virulence of Aeromonas salmonicida. (curehunter.com)
  • In this study, the genes encoding GCAT ( satA ) and AspA (aspA) have been cloned and mutagenized by marker replacement of internal deletions, and the constructs have been used for the creation of isogenic satA and aspA mutants of A. salmonicida. (curehunter.com)
  • Two A. salmonicida mutants possessing the A-layer in different disorganized states had a reduced ability to interact with M phi. (mysciencework.com)
  • Comparison of whole genome phylogeny and A-layer typing for a subset of strains further resulted in compatible tree topologies, indicating the utility of vapA as a phylogenetic as well as an epizootiological marker in A. salmonicida. (wur.nl)
  • A significant correlation was found between the level of A. salmonicida-specific antibodies measured prior to challenge and the endpoint survival of each group after the experimental infection, and furthermore, the levels of these antibodies remained elevated for at least 14 months post vaccination. (ovid.com)
  • Investigations of the lipopolysaccharide fractions from Aeromonassalmonicida smooth and rough forms. (microbiologyresearch.org)
  • Some clinical furunculosis survivors of an infected trout population became A. salmonicida carriers. (wikipedia.org)
  • Only A+ A. salmonicida cells were highly cytotoxic to trout M phi, especially after being coated with hemin, presumably due to a more focused targeting of the bacterial cell onto the M phi surface and/or into the intracellular regions of the M phi. (mysciencework.com)
  • Three kind of hemolysin genes were cloned and analyzed from A.salmonicida. (nii.ac.jp)
  • 5. Aeromonadaceae representative (Aeromonas salmonicida). (buecher.de)
  • Induzimos resistência in vitro para ampicilina em A. salmonicida CECT894T (AsWT) obtendo uma estirpe resistente (AsR). (ua.pt)
  • While observing chum salmon in a density of 14.7 fish per square meter, 12.4% were infected with A. salmonicida, whereas, densities at 4.9 fish per square meter were infection-free. (wikipedia.org)
  • This review summarizes the results of studies on atypical Aeromonas salmonicida (aAS) -infection among farmed Arctic charr (Salvelinus alpinus) and European grayling (Thymallus thymallus) (later referred as charr and grayling, respectively). (jyu.fi)
  • This study was conducted to test the effectiveness of ciprofloxacin in the treatment of A. salmonicida infection. (journalcra.com)
  • salmonicida has now been a known threat to aquaculture for more than a century. (dtu.dk)
  • In conclusion, we found that A. salmonicida quickly responds to a β-lactam challenge, activating their intrinsic mechanisms of resistance and concurrently producing an adaptive response involving different processes, such as "persister" phenotype (reduced growth), growth in biofilm-mode, SOS response with eventual hypermutation. (ua.pt)
  • Recent work has described an R-plasmid mediated resistance in many of the resistant A. salmonicida strains. (nasa.gov)
  • salmonicida grown at elevated temperatures, i.e. 30-37°C. The appearance of motility was also accompanied by variation in sugar fermentation patterns, the loss of ability to degrade complex molecules and an increase in antibiotic resistance. (coursehero.com)
  • These changes indicated that exposure to A. salmonicida induced a characteristic biochemical response which could be used to determine the health status of salmon. (canada.ca)
  • When injected in combination with an A. salmonicida O-antigen bacterin, the non-specific factors were further elevated, and the specific response was raised over samples taken from fish given the bacterin without the immunostimulants. (elsevier.com)
  • The oxidase-negative strains formed a distinct group which differed from oxidase-positive atypical A. salmonicida type biochemically and in terms of ribopatterns. (nih.gov)