Aequorin
Scyphozoa
Luminescent Measurements
Ferrets
Calcium
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
Luminescence
Luminescent Proteins
Cnidaria
Thoracica
Papillary Muscles
Carnivora
Apoproteins
Bupranolol
Luminescent Agents
Caffeine
A methylxanthine naturally occurring in some beverages and also used as a pharmacological agent. Caffeine's most notable pharmacological effect is as a central nervous system stimulant, increasing alertness and producing agitation. It also relaxes SMOOTH MUSCLE, stimulates CARDIAC MUSCLE, stimulates DIURESIS, and appears to be useful in the treatment of some types of headache. Several cellular actions of caffeine have been observed, but it is not entirely clear how each contributes to its pharmacological profile. Among the most important are inhibition of cyclic nucleotide PHOSPHODIESTERASES, antagonism of ADENOSINE RECEPTORS, and modulation of intracellular calcium handling.
Strophanthidin
Firefly Luciferin
Arsenazo III
Ryanodine
A methylpyrrole-carboxylate from RYANIA that disrupts the RYANODINE RECEPTOR CALCIUM RELEASE CHANNEL to modify CALCIUM release from SARCOPLASMIC RETICULUM resulting in alteration of MUSCLE CONTRACTION. It was previously used in INSECTICIDES. It is used experimentally in conjunction with THAPSIGARGIN and other inhibitors of CALCIUM ATPASE uptake of calcium into SARCOPLASMIC RETICULUM.
Intracellular trafficking pathways in the assembly of connexins into gap junctions. (1/417)
Trafficking pathways underlying the assembly of connexins into gap junctions were examined using living COS-7 cells expressing a range of connexin-aequorin (Cx-Aeq) chimeras. By measuring the chemiluminescence of the aequorin fusion partner, the translocation of oligomerized connexins from intracellular stores to the plasma membrane was shown to occur at different rates that depended on the connexin isoform. Treatment of COS-7 cells expressing Cx32-Aeq and Cx43-Aeq with brefeldin A inhibited the movement of these chimera to the plasma membrane by 84 +/- 4 and 88 +/- 4%, respectively. Nocodazole treatment of the cells expressing Cx32-Aeq and Cx43-Aeq produced 29 +/- 16 and 4 +/- 7% inhibition, respectively. In contrast, the transport of Cx26 to the plasma membrane, studied using a construct (Cx26/43T-Aeq) in which the short cytoplasmic carboxyl-terminal tail of Cx26 was replaced with the extended carboxyl terminus of Cx43, was inhibited 89 +/- 5% by nocodazole and was minimally affected by exposure of cells to brefeldin A (17 +/-11%). The transfer of Lucifer yellow across gap junctions between cells expressing wild-type Cx32, Cx43, and the corresponding Cx32-Aeq and Cx43-Aeq chimeras was reduced by nocodazole treatment and abolished by brefeldin A treatment. However, the extent of dye coupling between cells expressing wild-type Cx26 or the Cx26/43T-Aeq chimeras was not significantly affected by brefeldin A treatment, but after nocodazole treatment, transfer of dye to neighboring cells was greatly reduced. These contrasting effects of brefeldin A and nocodazole on the trafficking properties and intercellular dye transfer are interpreted to suggest that two pathways contribute to the routing of connexins to the gap junction. (+info)Reactive oxygen metabolites increase mitochondrial calcium in endothelial cells: implication of the Ca2+/Na+ exchanger. (2/417)
In endothelial cells, a bolus of hydrogen peroxide (H2O2) or oxygen metabolites generated by hypoxanthine-xanthine oxidase (HX-XO) increased the mitochondrial calcium concentration [Ca2+]m. Both agents caused a biphasic increase in [Ca2+]m which was preceded by a rise in cytosolic free calcium concentration [Ca2+]c (18 and 6 seconds for H2O2 and HX-XO, respectively). The peak and plateau elevations of [Ca2+] were consistently higher in the mitochondrial matrix than in the cytosol. In Ca2+-free/EGTA medium, the plateau phase of elevated [Ca2+] evoked by H2O2 due to capacitative Ca2+ influx was abolished in the cytosol, but was maintained in the mitochondria. In contrast to H2O2 and HX-XO, ATP which binds the P2Y purinoceptors induced an increase in [Ca2+]m that was similar to that of [Ca2+]c. When cells were first stimulated with inositol 1,4, 5-trisphosphate-generating agonists or the Ca2+-ATPase inhibitor cyclopiazonic acid (CPA), subsequent addition of H2O2 did not affect [Ca2+]c, but still caused an elevation of [Ca2+]m. Moreover, the specific inhibitor of the mitochondrial Ca2+/Na+ exchanger, 7-chloro-3,5-dihydro-5-phenyl-1H-4.1-benzothiazepine-2-on (CGP37157), did not potentiate the effects of H2O2 and HX-XO on [Ca2+]m, while causing a marked increase in the peak [Ca2+]m and a significant attenuation of the rate of [Ca2+]m efflux upon addition of histamine or CPA. In permeabilized cells, H2O2 mimicked the effects of CGP37157 causing an increase in the basal level of matrix free Ca2+ and decreased efflux. Dissipation of the electrochemical proton gradient by carbonylcyanide p-(trifluoromethoxy) phenylhydrazone (FCCP), and blocade of the Ca2+ uptake by ruthenium red prevented [Ca2+]m increases evoked by H2O2. These results demonstrate that the H2O2-induced elevation in [Ca2+]m results from a transfer of Ca2+ secondary to increased [Ca2+]c, and an inhibition of the Ca2+/Na+ electroneutral exchanger of the mitochondria. (+info)Functional effects of endothelin and regulation of endothelin receptors in isolated human nonfailing and failing myocardium. (3/417)
BACKGROUND: An activated endothelin (ET) system may be of pathophysiological relevance in human heart failure. We characterized the functional effects of ET-1, ET receptors, and ET-1 peptide concentration in left ventricular myocardium from 10 nonfailing hearts (NF) and 27 hearts in end-stage failure due to idiopathic dilative cardiomyopathy (DCM). METHODS AND RESULTS: Inotropic effects were characterized in isolated muscle strips (1 Hz; 37 degrees C). ET-1 0.0001 to 0.3 micromol/L significantly (P<0.05) increased twitch force by maximally 59+/-10% in NF and by 36+/-11% in DCM (P<0.05 versus NF). Preincubation with propranolol 1 micromol/L and prazosin 0.1 micromol/L did not affect the response to ET-1, but the mixed ET receptor antagonist bosentan and the ETA receptor antagonist BQ-123 shifted the concentration-response curves for ET-1 rightward. The ETB receptor agonist sarafotoxin S6c 0.001 to 0.3 micromol/L had no functional effects. The inotropic response to ET-1 was not associated with increased intracellular Ca2+ transients, as assessed in aequorin-loaded muscle strips. ET receptor density (Bmax; radioligand binding) was 62.5+/-12.5 fmol/mg protein in NF and 122. 4+/-24.3 fmol/mg protein in DCM (P<0.05 versus NF). The increase in Bmax in DCM resulted from an increase in ETA receptors without change in ETB receptors. ET-1 peptide concentration (radioimmunoassay) was higher in DCM than in NF (14 447+/-2232 versus 4541+/-1340 pg/mg protein, P<0.05). CONCLUSIONS: ET-1 exerts inotropic effects in human myocardium through ETA receptor-mediated increases in myofibrillar Ca2+ responsiveness. In DCM, functional effects of ET-1 are attenuated, but ETA receptor density and ET-1 peptide concentration are increased, indicating an activated local cardiac ET system and possibly a reduced postreceptor signaling efficiency. (+info)Secretagogues modulate the calcium concentration in the endoplasmic reticulum of insulin-secreting cells. Studies in aequorin-expressing intact and permeabilized ins-1 cells. (4/417)
The precise regulation of the Ca2+ concentration in the endoplasmic reticulum ([Ca2+]er) is important for protein processing and signal transduction. In the pancreatic beta-cell, dysregulation of [Ca2+]er may cause impaired insulin secretion. The Ca2+-sensitive photoprotein aequorin mutated to lower its Ca2+ affinity was stably expressed in the endoplasmic reticulum (ER) of rat insulinoma INS-1 cells. The steady state [Ca2+]er was 267 +/- 9 microM. Both the Ca2+-ATPase inhibitor cyclopiazonic acid and 4-chloro-m-cresol, an activator of ryanodine receptors, caused an almost complete emptying of ER Ca2+. The inositol 1,4,5-trisphosphate generating agonists, carbachol, and ATP, reduced [Ca2+]er by 20-25%. Insulin secretagogues that raise cytosolic [Ca2+] by membrane depolarization increased [Ca2+]er in the potency order K+ >> glucose > leucine, paralleling their actions in the cytosolic compartment. Glucose, which augmented [Ca2+]er by about 25%, potentiated the Ca2+-mobilizing effect of carbachol, explaining the corresponding observation in cytosolic [Ca2+]. The filling of ER Ca2+ by glucose is not directly mediated by ATP production as shown by the continuous monitoring of cytosolic ATP in luciferase expressing cells. Both glucose and K+ increase [Ca2+]er, but only the former generated whereas the latter consumed ATP. Nonetheless, drastic lowering of cellular ATP with a mitochondrial uncoupler resulted in a marked decrease in [Ca2+]er, emphasizing the requirement for mitochondrially derived ATP above a critical threshold concentration. Using alpha-toxin permeabilized cells in the presence of ATP, glucose 6-phosphate did not change [Ca2+]er, invalidating the hypothesis that glucose acts through this metabolite. Therefore, insulin secretagogues that primarily stimulate Ca2+ influx, elevate [Ca2+]er to ensure beta-cell homeostasis. (+info)Reactive oxygen species activate a Ca2+-dependent cell death pathway in the unicellular organism Trypanosoma brucei brucei. (5/417)
Here we examine a cell death process induced by reactive oxygen species (ROS) in the haemoflagellate Trypanosoma brucei brucei. Ca2+ distribution in cellular compartments was measured with stable transformants expressing aequorin targeted to the cytosol, nucleus or mitochondrion. Within 1.5 h of ROS production, mitochondrial Ca2+ transport was impaired and the Ca2+ barrier between the nuclear envelope and cytosol was disrupted. Consequently the mitochondrion did not accumulate Ca2+ efficiently in response to an extracellular stimulus, and excess Ca2+ accumulated in the nucleus. The terminal transferase deoxytidyl uridine end labelling assay revealed that, 5 h after treatment with ROS, extensive fragmentation of nuclear DNA occurred in over 90% of the cells. Permeability changes in the plasma membrane did not occur until an additional 2 h had elapsed. The intracellular Ca2+ buffer, EGTA acetoxymethyl ester, prevented DNA fragmentation and prolonged the onset of changes in cell permeability. Despite some similarities to apoptosis, nuclease activation was not a consequence of caspase 3, caspase 1, calpain, serine protease, cysteine protease or proteasome activity. Moreover, trypanosomes expressing mouse Bcl-2 were not protected from ROS even though protection from mitochondrial dysfunction and ROS have been reported for mammalian cells. Overall, these results demonstrate that Ca2+ pathways can induce pathology in trypanosomes, although the specific proteins involved might be distinct from those in metazoans. (+info)The vacuolar Ca2+/H+ exchanger Vcx1p/Hum1p tightly controls cytosolic Ca2+ levels in S. cerevisiae. (6/417)
It is well established that the vacuole plays an important role in the cellular adaptation to growth in the presence of elevated extracellular Ca2+ concentrations in Saccharomyces cerevisiae. The Ca2+ ATPase Pmc1p and the Ca2+/H+ exchanger Vcx1p/Hum1p have been shown to facilitate Ca2+ sequestration into the vacuole. However, the distinct physiological roles of these two vacuolar Ca2+ transporters remain uncertain. Here we show that Vcx1p can rapidly sequester a sudden pulse of cytosolic Ca2+ into the vacuole, while Pmc1p carries out this function much less efficiently. This finding is consistent with the postulated role of Vcx1p as a high capacity, low affinity Ca2+ transporter and suggests that Vcx1p may act to attenuate the propagation of Ca2+ signals in this organism. (+info)Differential pharmacological properties and signal transduction of the sphingosine 1-phosphate receptors EDG-1, EDG-3, and EDG-5. (7/417)
Sphingosine 1-phosphate (SPP) is a potent lipid mediator released upon cellular activation. In this report, pharmacological properties of the three G-protein-coupled receptors (GPCRs) for SPP, EDG-1, -3, and -5 are characterized using a Xenopus oocyte expression system, which lacks endogenous SPP receptors. Microinjection of the EDG-3 and EDG-5 but not EDG-1 mRNA conferred SPP-responsive intracellular calcium transients; however, the EDG-5 response was quantitatively much less. Co-expression of EDG-1 receptor with the chimeric Galphaqi protein conferred SPP responsiveness. Galphaqi or Galphaq co-injection also potentiated the EDG-5 and EDG-3 mediated responses to SPP. These data suggest that SPP receptors couple differentially to the Gq and Gi pathway. All three GPCRs were also activated by sphingosylphosphorylcholine, albeit at higher concentrations. None of the other related sphingolipids tested stimulated or blocked SPP-induced calcium responses. However, suramin, a polycyclic anionic compound, selectively antagonized SPP-activated calcium transients in EDG-3 expressing oocytes with an IC50 of 22 microM, suggesting that it is an antagonist selective for the EDG-3 GPCR isotype. We conclude that the three SPP receptors signal differentially by coupling to different G-proteins. Furthermore, because only EDG-3 was antagonized by suramin, variations in receptor structure may determine differences in antagonist selectivity. This property may be exploited to synthesize receptor subtype-specific antagonists. (+info)Measurement of perimitochondrial Ca2+ concentration in bovine adrenal glomerulosa cells with aequorin targeted to the outer mitochondrial membrane. (8/417)
Microdomains of high cytosolic free Ca(2+) concentration in the proximity of mitochondria might have an important role in the stimulation of steroidogenesis in bovine adrenal glomerulosa cells. In the present study we have investigated local changes of free Ca(2+) concentration near the outer mitochondrial membrane ([Ca(2+)](om)) under stimulation with angiotensin II (Ang II) and K(+). Glomerulosa cells in primary culture were transfected with a recombinant cDNA encoding the N-terminal region of the human translocase protein 20 of the outer mitochondrial membrane, in frame with the Ca(2+)-sensitive photoprotein aequorin. This chimaeric aequorin (TomAeq) was associated with mitochondria-enriched subcellular fractions of transfected COS-7 cells and was susceptible to proteinase K, showing that it was targeted to the outer mitochondrial membrane, facing the cytosolic space. In bovine adrenal glomerulosa cells transfected with TomAeq cDNA, Ang II induced a transient [Ca(2+)](om) peak reaching 1.42+/-0.28 microM, which decreased immediately to the basal resting value. The peak response to Ang II was strikingly lower than the peak response of mitochondrial free Ca(2+) concentration, which increased to 5.4+/-1.2 microM. The smaller response of [Ca(2+)](om) to Ang II compared with the elevated matrix response did not result from buffering effects of the organelle, from altered mechanisms of intramitochondrial Ca(2+) transport or from differences in the affinity of the chimaeric aequorins for Ca(2+). This approach has allowed us to follow perimitochondrial Ca(2+) homeostasis in bovine glomerulosa cells under stimulation with Ca(2+)-mobilizing agonists and to reveal a strong gradient of Ca(2+) concentration between the mitochondrial matrix and the immediate environment of the organelle. (+info)
Changes in Intracellular Free Calcium Concentration during Illumination of Invertebrate Photoreceptors | JGP
Aequorin Mutants with Site-Specifically Incorporated Non-Natural Amino by Kristen Marie Grinstead
Cell lines expressing recombinant aequorin and a G-protein coupled receptor for functional screening ( INTRODUCTION ...
Similar papers for Changes in growth patterns and intracellular calcium concentrations in Aspergillus awamori treated with...
1emm - Proteopedia, life in 3D
PromoKine - Luminescent Calcium Indicators
Mitochondrial Ca2+ homeostasis in intact cells. | JCB
Microdomains with high Ca2+ close to IP3-sensitive channels that are sensed by neighboring mitochondria | Science
Quincy Bioscience
Apoaequorin Reviews - Does It Really Work & Is It Safe To Use?
Adenosine 5′-[α β-methylene]triphosphate potentiates the oscillatory cytosolic Ca2+ responses of hepatocytes to ATP, but not to...
Comparative analysis of MAMP-induced calcium influx in arabidopsis seedlings and protoplasts
Subcortical Ca2+ Waves Sneaking Under the Plasma Membrane in Endothelial Cells | Circulation Research
Subcortical Ca2+ Waves Sneaking Under the Plasma Membrane in Endothelial Cells | Circulation Research
Proposed new fluorpro charter -- comments welcome
Understanding Appetite using HiBiT Protein Tagging
Gentaur Molecular :Biotium \ COELENTERAZINE HCP \ 10113
Human HCRTR2/Apoaequorin Stable Cell Line-CHO - Creative Biogene
Prediction of bioluminescent proteins by using sequence-derived features and lineage-specific scheme | BMC Bioinformatics |...
Granules as Calcium Stores | Science Signaling
Principle Microbiology: 2011
AID 632132 - Antagonist activity at rat MCHR1 expressed in HEK293 cells by aequorin bioluminescence assay - PubChem
Safety assessment of Apoaequorin, a protein preparation: subchronic toxicity study in rats. | CureHunter
Patterns of free calcium in zebrafish embryos | Journal of Cell Science
Uncategorized - Page 2 - medntel.com
writing staff - Page 2 - medntel.com
FDSS7000EX Functional Drug Screening System | 浜松ホトニクス
Denver Doc Online
The role of Na(+)-Ca2+ exchange in paired pulse potentiation o...
Genomics, Medicine, and Pseudoscience: September 2015
Genomics, Medicine, and Pseudoscience: September 2015
Semi-Synthetic Organism Uses New DNA Bases | IFLScience
Development of novel hybridization assays and immunoassays based on bi by Eleftheria. Laios
SciCombinator - Genomic organization, evolution, and expression of photoprotein and opsin genes in Mnemiopsis leidyi: a new...
Viladevall L~Arino J, 2004 / Papers / YeastPhenome.org
Application of BRET to monitor ligand binding to GPCRs - Nottingham ePrints
Contrasting effects of ischemia on the kinetics of membrane voltage and intracellular calcium transient underlie electrical...
malignant leptomeningeal tumor drug therapy 2000:2010[pubdate] *count=100 - BioMedLib™ search engine
Patent US6316267 - Luminescent protein stains an their method of use - Google Patents
Luminescent Proteins | Applied and Environmental Microbiology
anthos Mikrosysteme GmbH Online - LUmo 13101100
5-Hydroxytryptamine 5-HT3A (Serotonin) AequoScreen Cell Line | PerkinElmer
Human PTGER4/Clytin Stable Cell Line-HEK293 CSC-RG0742 - Creative BioMart
CHO (+Gα16, medium level) AequoScreen parental cell line | PerkinElmer
Can pharmacy memory supplements actually help? - Ninche AG
Biology-Online • View topic - The Fiber Disease
Catalog - Hamamatsu
Purinergic P2Y11 AequoScreen Cell Line | PerkinElmer
Morestan | Semantic Scholar
When Do Ferrets Shed: 10 FAQs Answered - trifl.org
Aequorin
Chemical characterization of aequorin indicates the protein is somewhat resilient to harsh treatments. Aequorin is heat ... Shimomura O, Inouye S, Musicki B, Kishi Y (1990). "Recombinant aequorin and recombinant semi-synthetic aequorins. Cellular Ca2+ ... making aequorin suitable as a Ca2+ reporter in plants, fungi, and mammalian cells. Aequorin has a number of advantages over ... not aequorin, although both originally derive from the same animal. Apoaequorin, the protein portion of aequorin, is an ...
Bioluminescence
Other cofactors may be required, such as calcium (Ca2+) for the photoprotein aequorin, or magnesium (Mg2+) ions and ATP for the ... Furthermore, some of the blue light released by aequorin in contact with calcium ions is absorbed by a green fluorescent ... ISBN 978-1-139-45181-9. Shimomura, O. (August 1995). "A short story of aequorin". The Biological Bulletin. 189 (1): 1-5. doi: ... Shimomura, O.; Johnson, F. H.; Saiga, Y. (1962). "Extraction, purification and properties of aequorin, a bioluminescent protein ...
Aequorea victoria
The species is best known as the source of two proteins involved in bioluminescence, aequorin, a photoprotein, and green ... PDB: 1EMA Shimomura O (August 1995). "A short story of aequorin". The Biological Bulletin. 189 (1): 1-5. doi:10.2307/1542194. ... In 1967, Ridgeway and Ashley microinjected aequorin into single muscle fibers of barnacles, and observed transient calcium ion- ... In 1961, Shimomura and Johnson isolated the protein aequorin, and its small molecule cofactor, coelenterazine, from large ...
Photoprotein
For example, the photoprotein aequorin produces a flash of light when luciferin and calcium are added, rather than the ... Because of the kinetically slow step, each aequorin molecule must "recharge" with another molecule of luciferin before it can ... Shimomura O, Johnson FH (1975). "Regeneration of the photoprotein aequorin". Nature. 256 (5514): 236-238. Bibcode:1975Natur.256 ... often until the addition of another required factor such as Ca2+ in the case of aequorin. Shimomura, O. "Bioluminescence: ...
Douglas Prasher
He is known for his work to clone and sequence the genes for the photoprotein aequorin and green fluorescent protein (GFP) and ... Prasher, D., McCann, R.O., Cormier, M.J., Cloning and expression of the cDNA coding for aequorin, a bioluminescent calcium- ... aequorin. Photochem. Photobiol., 49(4), 509-512 (1989). Prasher, D.C., O'Kane, D., Lee, J., Woodward, B., The lumazine protein ... where he identified the gene sequence for aequorin. He then joined the Biology Department of the Woods Hole Oceanographic ...
Bioreporter
Aequorin has been incorporated into human B cell lines for the detection of pathogenic bacteria and viruses in what is referred ... GFP, like aequorin, produces a blue fluorescent signal, but without the required addition of an exogenous substrate. All that ... Aequorin is a photoprotein isolated from the bioluminescent jellyfish Aequorea victoria. Upon addition of calcium ions (Ca2+) ... In some instances, the signal only occurs when a secondary substrate is added to the bioassay (luxAB, Luc, and aequorin). For ...
Intracellular delivery
Borle, A (1986). "A Simple Method for Incorporating Aequorin into Mammalian-Cells". American Journal of Physiology. 251 (2): ...
John Woodland Hastings
Hastings, J.W.; Mitchell, G.W.; Mattingly, P.H.; Blinks, J.R.; Van Leeuwen, M. (1969). "Response of aequorin bioluminescence to ... aequorin), which alone emits blue light, to a secondary green emitter which they termed green fluorescent protein (GFP). Once ...
Aequorea vitrina
Feeds on brine shrimp (Artemia salina) and rotifers (Brachionus plicatilis). It is bioluminescent due to aequorin and green ...
Calcium imaging
Colocalization of aequorin with GFP facilitates BRET/CRET (Bioluminescence or Chemiluminescence Resonance Energy Transfer), ... Such systems may rely on aequorin and the luciferin coelenterazine. Ca2+ binding causes a conformational change that ... "Chimeric green fluorescent protein-aequorin as bioluminescent Ca2+ reporters at the single-cell level". Proceedings of the ...
Connexin
George CH, Kendall JM, Campbell AK, Evans WH (November 1998). "Connexin-aequorin chimerae report cytoplasmic calcium ... "Assembly of chimeric connexin-aequorin proteins into functional gap junction channels. Reporting intracellular and plasma ...
Calcein
Alternatively, Fura-2 , Furaptra , Indo-1 and aequorin may be used. An acetomethoxy group obscures the part of the molecule ...
Roger Y. Tsien
Aequorin is also a useful tool to indicate calcium level inside cells; however, it has some limitations, primarily is that its ...
Green fluorescent protein
GFP is co-expressed with aequorin in small granules around the rim of the jellyfish bell. The secondary excitation peak (480 nm ... In the 1960s and 1970s, GFP, along with the separate luminescent protein aequorin (an enzyme that catalyzes the breakdown of ... In A. victoria, GFP fluorescence occurs when aequorin interacts with Ca2+ ions, inducing a blue glow. Some of this luminescent ... The purpose of both the (primary) bioluminescence (from aequorin's action on luciferin) and the (secondary) fluorescence of GFP ...
History of science and technology in Japan
... along with the separate luminescent protein aequorin (an enzyme that catalyzes the breakdown of luciferin, releasing light), ... purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea". Journal of ...
Jellyfish
Shimomura, O.; Johnson, F. H.; Saiga, Y. (1962). "Extraction, purification and properties of aequorin, a bioluminescent protein ... In 1961, Osamu Shimomura extracted green fluorescent protein (GFP) and another bioluminescent protein, called aequorin, from ...
Fusion gene
Prendergast FG, Mann KG (August 1978). "Chemical and physical properties of aequorin and the green fluorescent protein isolated ...
Aequorea forskalea
Despite containing a bioluminescent protein, aequorin, this species (as well as all other species in the genus) are almost ... Prendergast, Franklyn G.; Mann, Kenneth G. (1978-08-22). "Chemical and physical properties of aequorin and the green ... and extracted aequorin and GFP from various Aequorea species including A. forskalea. To this day, A. victoria is still the ... aequorin) and GFP (green fluorescent protein) discovered and studied extensively by Dr. Osamu Shimomura in 1961. While this ...
Cell CANARY
At the final step of these reactions, Ca2+ ions are released, and in the presence of aequorin, photons are emitted. Aequorin is ... Aequorin," Photochem. Photobiol., vol. 49, no. 4, 1989, pp. 509-512. Petrovick, Martha S., James D. Harper, Frances E. Nargi, ...
ILCD
Aequorin-expressing yeast emits light under electric control.J Biotechnol. 2011 Mar 20;152(3):93-5. Official website J ... It is based on yeast cells expressing aequorin protein sensitive to change in intracellular calcium. Upon electrical ...
Luciferin
It is the prosthetic group in the protein aequorin responsible for the blue light emission. Dinoflagellate luciferin is a ...
Adult neurogenesis
Prendergast, Franklyn G.; Mann, Kenneth G. (1978-08-22). "Chemical and physical properties of aequorin and the green ...
Chemiluminescence
Another protein, aequorin, found in certain jellyfish, produces blue light in the presence of calcium. It can be used in ...
Calcium concentration microdomains
... can be visualised with fluorescence microscopy by using aequorin as a reporter protein. The ...
K-Strophanthidin
"The effects of digitalis on intracellular calcium transients in mammalian working myocardium as detected with aequorin". ...
Steven M. Smith
Knight, Marc R.; Campbell, Anthony K.; Smith, Steven M.; Trewavas, Anthony J. (8 August 1991). "Transgenic plant aequorin ... aequorin, to report calcium signalling in plants. Together they obtained funding, created the plants and showed that they could ...
Anthony Trewavas
Knight, M. R.; Campbell, A. K.; Smith, S. M.; Trewavas, A. J. (1991). "Transgenic plant aequorin reports the effects of touch ...
EF hand
In addition, aequorin has been used for years as an indicator of Ca2+ and has been shown to be safe and well tolerated by cells ... Aequorin belongs to the EF-hand family of CaBPs, with EF-hand loops that are closely related to CaBPs in mammals. ... Aequorin is made up of two components - the calcium binding component apoaequorin (AQ) and the chemiluminescent molecule ... EPS15 homology (EH) domain - InterPro: IPR000261 Aequorin is a calcium binding protein (CaBP) isolated from the cnidarian ...
UR-AK49
"Determination of affinity and activity of ligands at the human neuropeptide Y Y4 receptor by flow cytometry and aequorin ...
Osamu Shimomura
In 1962, their work culminated in the discovery of the proteins aequorin and green fluorescent protein (GFP) in A. victoria; ...
Aequorin's
Lair |
Flight Rising
Erratum: Effects of OR-1896, an active metabolite of levosimendan, on contractile force and aequorin light transients in intact...
T2 - Effects of OR-1896, an active metabolite of levosimendan, on contractile force and aequorin light transients in intact ... Erratum: Effects of OR-1896, an active metabolite of levosimendan, on contractile force and aequorin light transients in intact ... title = "Erratum: Effects of OR-1896, an active metabolite of levosimendan, on contractile force and aequorin light transients ... Erratum : Effects of OR-1896, an active metabolite of levosimendan, on contractile force and aequorin light transients in ...
A new short-term toxicity assay using Aspergillus awamori with recombinant aequorin gene | BMC Microbiology | Full Text
The recombinant aequorin gene from the jellyfish Aequorea victoria responsible for the expression of the Ca2+-sensitive ... aequorin photoprotein has been cloned in the filamentous fungus Aspergillus awamori. This has allowed real life monitoring of [ ... bioassay utilizing eukaryotic cells has been developed based on filamentous fungi transformed with the recombinant aequorin ... Recently recombinant aequorin gene has been expressed in filamentous fungi [10]. Aequorin is a Ca2+-sensitive photoprotein of ...
ANTICA TERRA Chardonnay, Willamette Valley 'Aequorin' 2019 - International Wine Report
Team:TzuChiU Formosa/background - 2009.igem.org
And when Ca2+ ions bind on aequorin, the aequorin will emit a glowing blue light and GFP will raise to excited state and emit ... Aequorin and GFP(Green Fluorescent Protein)has existed for more than one hundred millions years and were discovered only in one ... Aequorin is composed of two distinct units: a) the apoprotein: apoaequorin and b) the prosthetic group: coelenterazine (a ... In our research we use Aequorin-GFP fusion protein to be strong enough to illuminat and to replace streetlamp and hope one day ...
INTERCHIM: Vectors (Expression V., siRNA,...)
Why A Little Green Signaling Protein Prompted This Year's No... : Neurology Today
He found that calcium ions activate a jellyfish protein called aequorin. Once activated, aequorin produces blue light, but if ... Cloning and expression of the Cdna coding for aequorin, a bioluminescent calcium-binding protein. Biochem Biophys Res Comm 1985 ... Shimomura O, Johnson FH, Y Saiga Y. Extraction, purification and properties of aequorin, a bioluminescent protein from the ...
Search | The Embryo Project Encyclopedia
Find Research Projects - University of Edinburgh Research Explorer
Professor Kenneth Wann - People - Cardiff University
... and aequorin. This construct does in principle monitor levels of [Ca2+]i close to ion channel microdomains and, provide ... already transformed with firefly luciferase and transfected with the photoprotein aequorin, enabling changes in cytosolic free ... is one of the methods routinely used by cell biologists to measure free Ca2+ inside cells and in particular aequorin a ...
CIPSM - Research Area B
Comparison of Photina luminescent calcium mobilization assays on the FlexStation 3 reader and FLIPR Tetra System | Molecular...
The FLIPR Tetra System is now available with an aequorin option which includes a novel ICCD camera technology optimized for use ... A preconfigured aequorin assay protocol for the FlexStation 3 reader is available in the SoftMax Pro Software. ... The FlexStation 3 reader and the FLIPR Tetra System with aequorin option (ICCD camera) have been used to measure the Photina ... This application note provides a basic protocol for performing an adherent aequorin assay using the FlexStation 3 reader and ...
Team:Cambridge/BioluminescenceLinks - 2010.igem.org
Find Research outputs - Augusta University Research Profiles
Find Research outputs - Augusta University Research Profiles
Steven Smith - Profiles | University of Tasmania
Search | Page 3 | The Embryo Project Encyclopedia
Introduction to Fluorescent Proteins | Nikon's MicroscopyU
Likewise, Aequorea victoria green fluorescent protein is thought to participate in a tetrameric complex with aequorin, but this ... researchers determined that aequorin and the green fluorescent protein work together in the light organs of the jellyfish to ... which they named aequorin. During the isolation procedure, a second protein was observed that lacked the blue-emitting ... bioluminescent properties of aequorin, but was able to produce green fluorescence when illuminated with ultraviolet light. Due ...
OT receptor | Vasopressin and oxytocin receptors | IUPHAR/BPS Guide to PHARMACOLOGY
Download Free As In Freedom 20 Richard Stallman And The Free Software Revolution 0
GtR
Provigil cost 2020 - No prescription pharmacy
Buy janumet online with free samples
D-Luciferin, Potassium Salt (Proven and Published®) | GoldBio
... and aequorins. It is commonly used to monitor reporter genes in BRET (Bioluminescence Resonance Energy Transfer), ELISA and HTS ... and aequorins. It is commonly used to monitor reporter genes in BRET (Bioluminescence Resonance Energy Transfer), ELISA and HTS ... and aequorins. It is commonly used to monitor reporter genes in BRET (Bioluminescence Resonance Energy Transfer), ELISA and HTS ... and aequorins. It is commonly used to monitor reporter genes in BRET (Bioluminescence Resonance Energy Transfer), ELISA and HTS ...
Dopamine/Ecdysteroid receptor
Buy cheap Oxytrol online # Canada Drugstore
PEPSIC - pepsic.bvsalud.org
Jellyfish6
- The recombinant aequorin gene from the jellyfish Aequorea victoria responsible for the expression of the Ca 2+ -sensitive aequorin photoprotein has been cloned in the filamentous fungus Aspergillus awamori . (biomedcentral.com)
- Aequorin and GFP(Green Fluorescent Protein)has existed for more than one hundred millions years and were discovered only in one jellyfish species-Aequorea Victoria. (igem.org)
- He found that calcium ions activate a jellyfish protein called aequorin. (lww.com)
- Osamu Shimomura and Frank Johnson, working at the Friday Harbor Laboratories of the University of Washington in 1961, first isolated a calcium-dependent bioluminescent protein from the Aequorea victoria jellyfish, which they named aequorin . (microscopyu.com)
- Over the next two decades, researchers determined that aequorin and the green fluorescent protein work together in the light organs of the jellyfish to convert calcium-induced luminescent signals into the green fluorescence characteristic of the species. (microscopyu.com)
- In A. victoria , aequorin emits blue light which excites GFP giving the overall green glow to the jellyfish 1 . (biologists.com)
Recombinant aequorin2
- A novel toxicity bioassay utilizing eukaryotic cells has been developed based on filamentous fungi transformed with the recombinant aequorin gene. (biomedcentral.com)
- Bioluminescence inmunoassay for cortisol using recombinant aequorin as a label. (bvsalud.org)
Photoprotein3
- Aequorin photoprotein (untagged) is recombinantly produced in E. coli, purified via multi-step chromatography and appears as a yellow liquid. (nanolight.com)
- Aequorin photoprotein (untagged) is recombinantly produced in E. coli, purified via multi-step chromatography and appears as a white lyophilized powder. (nanolight.com)
- b) [Ca2+]c measurement with fura-2 followed by quantitative immunocytochemistry of single cells and iii) [Ca2+]c measurement of cell population upon cotransfection with the Ca(2+)-sensitive photoprotein, aequorin. (rupress.org)
Bioluminescent2
- During the isolation procedure, a second protein was observed that lacked the blue-emitting bioluminescent properties of aequorin, but was able to produce green fluorescence when illuminated with ultraviolet light. (microscopyu.com)
- This study utilized the bioluminescent Ca2+-indicator GFP-aequorin to monitor the nicotine-induced Ca2+-response within the mushroom bodies (MB) , a higher-order brain center in flies, and examined how DopEcR modulates these Ca2+-dynamics. (sdbonline.org)
Protein7
- Once Ca 2+ ions are bound to the three Ca 2+ -binding sites in aequorin, the protein is converted into an oxygenase. (biomedcentral.com)
- In our research we use Aequorin-GFP fusion protein to be strong enough to illuminat and to replace streetlamp and hope one day it can substitute daylight lamp without use of electricity. (igem.org)
- Once activated, aequorin produces blue light, but if the green fluorescent protein is nearby, the two proteins together yield a bright green signal. (lww.com)
- At that time, they called this protein aequorin. (coursera.org)
- This distinct discovery happened in a very interesting chance and then, because he was studying the how to make this protein Aequorin to have light, at that time, and then he tried so many solutions and the combinations and he failed. (coursera.org)
- But one day, when he was about to finish his experiment, he throw out his solutions into the sink, and then, of course, his left over Aequorin, this protein into sink as well, so all of a sudden, he saw in the sink the protein has very strong light over there. (coursera.org)
- And then the first time, he realized this protein, Aequorin here, might be very useful for biological studies and research. (coursera.org)
Proteins1
- Two fluorescent proteins were identified in this study: the first, named aequorin, exhibited a blue glow in the presence of Ca 2+ ions. (biologists.com)
Emit2
- Then Aequorin can emit the blue light (wavelenght= 469 nm) and supply energy for GFP. (igem.org)
- Aequorin was "charged" with unmodified (native) Coelenterazine and will emit light at 465 nm upon Ca 2+ contact. (nanolight.com)
Assay1
- Description: Agonist activity at MmOT receptor expressed in HEK293FT cells assessed as stimulation of calcium release by Aequorin based assay. (guidetopharmacology.org)
Coelenterazine1
- Aequorin is composed of two distinct units: a) the apoprotein: apoaequorin and b) the prosthetic group: coelenterazine (a luciferin). (igem.org)
Scope1
- Polysciences) was used in calculation of the interactions between AvicFP1 and aequorin are beyond the scope of this species in the weak dimer interface in the. (gotonextstep.com)
Light1
- Endoh, M. / Erratum : Effects of OR-1896, an active metabolite of levosimendan, on contractile force and aequorin light transients in intact rabbit ventricular myocardium (Journal of Cardiovascular Pharmacology (2000) 36 (118-125)) . (elsevier.com)
Change1
- The review will publish desired to high change aequorin. (vanpanhuys.com)
Review1
- For your download Batı'ya Yön Veren Metinler- III Aydınlanma Burjuvazi Yüzyılı Bilim Çağının Zaferi (1650- 1800) 2010 , are soon place magnetic aequorin like review or Social Security documents. (coldbacon.com)