ADP-Ribosylation Factors: MONOMERIC GTP-BINDING PROTEINS that were initially recognized as allosteric activators of the MONO(ADP-RIBOSE) TRANSFERASE of the CHOLERA TOXIN catalytic subunit. They are involved in vesicle trafficking and activation of PHOSPHOLIPASE D. This enzyme was formerly listed as EC 3.6.1.47ADP-Ribosylation Factor 1: ADP-RIBOSYLATION FACTOR 1 is involved in regulating intracellular transport by modulating the interaction of coat proteins with organelle membranes in the early secretory pathway. It is a component of COAT PROTEIN COMPLEX I. This enzyme was formerly listed as EC 3.6.1.47.Brefeldin A: A fungal metabolite which is a macrocyclic lactone exhibiting a wide range of antibiotic activity.Coated Vesicles: Vesicles formed when cell-membrane coated pits (COATED PITS, CELL-MEMBRANE) invaginate and pinch off. The outer surface of these vesicles are covered with a lattice-like network of coat proteins, such as CLATHRIN, coat protein complex proteins, or CAVEOLINS.Coatomer Protein: A 700-kDa cytosolic protein complex consisting of seven equimolar subunits (alpha, beta, beta', gamma, delta, epsilon and zeta). COATOMER PROTEIN and ADP-RIBOSYLATION FACTOR 1 are principle components of COAT PROTEIN COMPLEX I and are involved in vesicle transport between the ENDOPLASMIC RETICULUM and the GOLGI APPARATUS.Guanine Nucleotide Exchange Factors: Protein factors that promote the exchange of GTP for GDP bound to GTP-BINDING PROTEINS.GTP-Binding Proteins: Regulatory proteins that act as molecular switches. They control a wide range of biological processes including: receptor signaling, intracellular signal transduction pathways, and protein synthesis. Their activity is regulated by factors that control their ability to bind to and hydrolyze GTP to GDP. EC 3.6.1.-.Coat Protein Complex I: A protein complex comprised of COATOMER PROTEIN and ADP RIBOSYLATION FACTOR 1. It is involved in transport of vesicles between the ENDOPLASMIC RETICULUM and the GOLGI APPARATUS.Golgi Apparatus: A stack of flattened vesicles that functions in posttranslational processing and sorting of proteins, receiving them from the rough ENDOPLASMIC RETICULUM and directing them to secretory vesicles, LYSOSOMES, or the CELL MEMBRANE. The movement of proteins takes place by transfer vesicles that bud off from the rough endoplasmic reticulum or Golgi apparatus and fuse with the Golgi, lysosomes or cell membrane. (From Glick, Glossary of Biochemistry and Molecular Biology, 1990)GTPase-Activating Proteins: Proteins that activate the GTPase of specific GTP-BINDING PROTEINS.Adenosine Diphosphate Ribose: An ester formed between the aldehydic carbon of RIBOSE and the terminal phosphate of ADENOSINE DIPHOSPHATE. It is produced by the hydrolysis of nicotinamide-adenine dinucleotide (NAD) by a variety of enzymes, some of which transfer an ADP-ribosyl group to target proteins.Phospholipase D: An enzyme found mostly in plant tissue. It hydrolyzes glycerophosphatidates with the formation of a phosphatidic acid and a nitrogenous base such as choline. This enzyme also catalyzes transphosphatidylation reactions. EC 3.1.4.4.Guanosine 5'-O-(3-Thiotriphosphate): Guanosine 5'-(trihydrogen diphosphate), monoanhydride with phosphorothioic acid. A stable GTP analog which enjoys a variety of physiological actions such as stimulation of guanine nucleotide-binding proteins, phosphoinositide hydrolysis, cyclic AMP accumulation, and activation of specific proto-oncogenes.Guanosine Triphosphate: Guanosine 5'-(tetrahydrogen triphosphate). A guanine nucleotide containing three phosphate groups esterified to the sugar moiety.Nucleoside Diphosphate SugarsAdenosine Diphosphate Sugars: Esters formed between the aldehydic carbon of sugars and the terminal phosphate of adenosine diphosphate.Endocytosis: Cellular uptake of extracellular materials within membrane-limited vacuoles or microvesicles. ENDOSOMES play a central role in endocytosis.Protein Transport: The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Ribose: A pentose active in biological systems usually in its D-form.Membrane Proteins: Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.HeLa Cells: The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.ADP Ribose Transferases: Enzymes that transfer the ADP-RIBOSE group of NAD or NADP to proteins or other small molecules. Transfer of ADP-ribose to water (i.e., hydrolysis) is catalyzed by the NADASES. The mono(ADP-ribose)transferases transfer a single ADP-ribose. POLY(ADP-RIBOSE) POLYMERASES transfer multiple units of ADP-ribose to protein targets, building POLY ADENOSINE DIPHOSPHATE RIBOSE in linear or branched chains.Carrier Proteins: Transport proteins that carry specific substances in the blood or across cell membranes.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Cholera Toxin: An ENTEROTOXIN from VIBRIO CHOLERAE. It consists of two major protomers, the heavy (H) or A subunit and the B protomer which consists of 5 light (L) or B subunits. The catalytic A subunit is proteolytically cleaved into fragments A1 and A2. The A1 fragment is a MONO(ADP-RIBOSE) TRANSFERASE. The B protomer binds cholera toxin to intestinal epithelial cells, and facilitates the uptake of the A1 fragment. The A1 catalyzed transfer of ADP-RIBOSE to the alpha subunits of heterotrimeric G PROTEINS activates the production of CYCLIC AMP. Increased levels of cyclic AMP are thought to modulate release of fluid and electrolytes from intestinal crypt cells.Recombinant Fusion Proteins: Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Pertussis Toxin: One of the virulence factors produced by BORDETELLA PERTUSSIS. It is a multimeric protein composed of five subunits S1 - S5. S1 contains mono ADPribose transferase activity.Virulence Factors, Bordetella: A set of BACTERIAL ADHESINS and TOXINS, BIOLOGICAL produced by BORDETELLA organisms that determine the pathogenesis of BORDETELLA INFECTIONS, such as WHOOPING COUGH. They include filamentous hemagglutinin; FIMBRIAE PROTEINS; pertactin; PERTUSSIS TOXIN; ADENYLATE CYCLASE TOXIN; dermonecrotic toxin; tracheal cytotoxin; Bordetella LIPOPOLYSACCHARIDES; and tracheal colonization factor.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Adenosine Diphosphate: Adenosine 5'-(trihydrogen diphosphate). An adenine nucleotide containing two phosphate groups esterified to the sugar moiety at the 5'-position.Poly(ADP-ribose) Polymerases: Enzymes that catalyze the transfer of multiple ADP-RIBOSE groups from nicotinamide-adenine dinucleotide (NAD) onto protein targets, thus building up a linear or branched homopolymer of repeating ADP-ribose units i.e., POLY ADENOSINE DIPHOSPHATE RIBOSE.Cell Membrane: The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.Botulinum Toxins: Toxic proteins produced from the species CLOSTRIDIUM BOTULINUM. The toxins are synthesized as a single peptide chain which is processed into a mature protein consisting of a heavy chain and light chain joined via a disulfide bond. The botulinum toxin light chain is a zinc-dependent protease which is released from the heavy chain upon ENDOCYTOSIS into PRESYNAPTIC NERVE ENDINGS. Once inside the cell the botulinum toxin light chain cleaves specific SNARE proteins which are essential for secretion of ACETYLCHOLINE by SYNAPTIC VESICLES. This inhibition of acetylcholine release results in muscular PARALYSIS.NAD: A coenzyme composed of ribosylnicotinamide 5'-diphosphate coupled to adenosine 5'-phosphate by pyrophosphate linkage. It is found widely in nature and is involved in numerous enzymatic reactions in which it serves as an electron carrier by being alternately oxidized (NAD+) and reduced (NADH). (Dorland, 27th ed)Adenylate Cyclase: An enzyme of the lyase class that catalyzes the formation of CYCLIC AMP and pyrophosphate from ATP. EC 4.6.1.1.Adenylate Cyclase Toxin: One of the virulence factors produced by virulent BORDETELLA organisms. It is a bifunctional protein with both ADENYLYL CYCLASES and hemolysin components.Poly Adenosine Diphosphate Ribose: A polynucleotide formed from the ADP-RIBOSE moiety of nicotinamide-adenine dinucleotide (NAD) by POLY(ADP-RIBOSE) POLYMERASES.Phosphatidylinositol 4,5-Diphosphate: A phosphoinositide present in all eukaryotic cells, particularly in the plasma membrane. It is the major substrate for receptor-stimulated phosphoinositidase C, with the consequent formation of inositol 1,4,5-triphosphate and diacylglycerol, and probably also for receptor-stimulated inositol phospholipid 3-kinase. (Kendrew, The Encyclopedia of Molecular Biology, 1994)Bacterial Toxins: Toxic substances formed in or elaborated by bacteria; they are usually proteins with high molecular weight and antigenicity; some are used as antibiotics and some to skin test for the presence of or susceptibility to certain diseases.Kinetics: The rate dynamics in chemical or physical systems.Adaptor Proteins, Vesicular Transport: A class of proteins involved in the transport of molecules via TRANSPORT VESICLES. They perform functions such as binding to the cell membrane, capturing cargo molecules and promoting the assembly of CLATHRIN. The majority of adaptor proteins exist as multi-subunit complexes, however monomeric varieties have also been found.rhoA GTP-Binding Protein: A RHO GTP-BINDING PROTEIN involved in regulating signal transduction pathways that control assembly of focal adhesions and actin stress fibers. This enzyme was formerly listed as EC 3.6.1.47.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Azaguanine: One of the early purine analogs showing antineoplastic activity. It functions as an antimetabolite and is easily incorporated into ribonucleic acids.Intracellular Membranes: Thin structures that encapsulate subcellular structures or ORGANELLES in EUKARYOTIC CELLS. They include a variety of membranes associated with the CELL NUCLEUS; the MITOCHONDRIA; the GOLGI APPARATUS; the ENDOPLASMIC RETICULUM; LYSOSOMES; PLASTIDS; and VACUOLES.Enzyme Activation: Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.Biological Transport: The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Proteins: Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.NAD+ NucleosidaseCloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Tankyrases: A group of telomere associated proteins that interact with TRF1 PROTEIN, contain ANKYRIN REPEATS and have poly(ADP-ribose) polymerase activity.Cricetinae: A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.CHO Cells: CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). The species is a favorite for cytogenetic studies because of its small chromosome number. The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells.Mitochondrial ADP, ATP Translocases: A class of nucleotide translocases found abundantly in mitochondria that function as integral components of the inner mitochondrial membrane. They facilitate the exchange of ADP and ATP between the cytosol and the mitochondria, thereby linking the subcellular compartments of ATP production to those of ATP utilization.Signal Transduction: The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.

Identification of a new Pyk2 target protein with Arf-GAP activity. (1/1111)

Protein tyrosine kinase Pyk2 is activated by a variety of G-protein-coupled receptors and by extracellular signals that elevate intracellular Ca2+ concentration. We have identified a new Pyk2 binding protein designated Pap. Pap is a multidomain protein composed of an N-terminal alpha-helical region with a coiled-coil motif, followed by a pleckstrin homology domain, an Arf-GAP domain, an ankyrin homology region, a proline-rich region, and a C-terminal SH3 domain. We demonstrate that Pap forms a stable complex with Pyk2 and that activation of Pyk2 leads to tyrosine phosphorylation of Pap in living cells. Immunofluorescence experiments demonstrate that Pap is localized in the Golgi apparatus and at the plasma membrane, where it is colocalized with Pyk2. In addition, in vitro recombinant Pap exhibits strong GTPase-activating protein (GAP) activity towards the small GTPases Arf1 and Arf5 and weak activity towards Arf6. Addition of recombinant Pap protein to Golgi preparations prevented Arf-dependent generation of post-Golgi vesicles in vitro. Moreover, overexpression of Pap in cultured cells reduced the constitutive secretion of a marker protein. We propose that Pap functions as a GAP for Arf and that Pyk2 may be involved in regulation of vesicular transport through its interaction with Pap.  (+info)

ARF6 requirement for Rac ruffling suggests a role for membrane trafficking in cortical actin rearrangements. (2/1111)

The ARF6 GTPase regulates a novel endosomal-plasma membrane recycling pathway and influences cortical actin remodeling. Here we examined the relationship between ARF6 and Rac1, a Rho family GTPase, implicated in cortical actin rearrangements. Endogenous Rac1 colocalized with ARF6 at the plasma membrane and on the ARF6 recycling endosome in untransfected HeLa and primary human fibroblast cells. In transfected HeLa cells Rac1 and ARF6 also colocalized. Cells expressing wild-type ARF6 or Rac1 formed actin-containing surface protrusions and membrane ruffles, respectively, upon treatment with the G protein activator aluminum fluoride. Aluminum fluoride-treatment of cells transfected with equivalent amounts of plasmid resulted in enhanced membrane ruffling, with protrusions appearing as Rac expression was lowered. Co-expression of the dominant negative, GTP binding-defective ARF6 T27N mutant inhibited the aluminum fluoride-induced ruffling observed in cells expressing Rac1, and the constitutive ruffling observed in cells expressing the activated Rac1 Q61L mutant. In contrast, co-expression of the GTP-binding-defective, T17N mutant of either Rac1 or Cdc42 with ARF6 did not inhibit the aluminum fluoride-induced surface protrusions, nor did inactivation of Rho with C3-transferase. These observations suggest that ARF6, a non-Rho family GTPase, can, by itself, alter cortical actin and can influence the ability of Rac1 to form lamellipodia, in part, by regulating its trafficking to the plasma membrane.  (+info)

Coupling of coat assembly and vesicle budding to packaging of putative cargo receptors. (3/1111)

COPI-coated vesicle budding from lipid bilayers whose composition resembles mammalian Golgi membranes requires coatomer, ARF, GTP, and cytoplasmic tails of putative cargo receptors (p24 family proteins) or membrane cargo proteins (containing the KKXX retrieval signal) emanating from the bilayer surface. Liposome-derived COPI-coated vesicles are similar to their native counterparts with respect to diameter, buoyant density, morphology, and the requirement for an elevated temperature for budding. These results suggest that a bivalent interaction of coatomer with membrane-bound ARF[GTP] and with the cytoplasmic tails of cargo or putative cargo receptors is the molecular basis of COPI coat assembly and provide a simple mechanism to couple uptake of cargo to transport vesicle formation.  (+info)

GCS1, an Arf guanosine triphosphatase-activating protein in Saccharomyces cerevisiae, is required for normal actin cytoskeletal organization in vivo and stimulates actin polymerization in vitro. (4/1111)

Recent cloning of a rat brain phosphatidylinositol 3,4, 5-trisphosphate binding protein, centaurin alpha, identified a novel gene family based on homology to an amino-terminal zinc-binding domain. In Saccharomyces cerevisiae, the protein with the highest homology to centaurin alpha is Gcs1p, the product of the GCS1 gene. GCS1 was originally identified as a gene conditionally required for the reentry of cells into the cell cycle after stationary phase growth. Gcs1p was previously characterized as a guanosine triphosphatase-activating protein for the small guanosine triphosphatase Arf1, and gcs1 mutants displayed vesicle-trafficking defects. Here, we have shown that similar to centaurin alpha, recombinant Gcs1p bound phosphoinositide-based affinity resins with high affinity and specificity. A novel GCS1 disruption strain (gcs1Delta) exhibited morphological defects, as well as mislocalization of cortical actin patches. gcs1Delta was hypersensitive to the actin monomer-sequestering drug, latrunculin-B. Synthetic lethality was observed between null alleles of GCS1 and SLA2, the gene encoding a protein involved in stabilization of the actin cytoskeleton. In addition, synthetic growth defects were observed between null alleles of GCS1 and SAC6, the gene encoding the yeast fimbrin homologue. Recombinant Gcs1p bound to actin filaments, stimulated actin polymerization, and inhibited actin depolymerization in vitro. These data provide in vivo and in vitro evidence that Gcs1p interacts directly with the actin cytoskeleton in S. cerevisiae.  (+info)

EFA6, a sec7 domain-containing exchange factor for ARF6, coordinates membrane recycling and actin cytoskeleton organization. (5/1111)

We have identified a human cDNA encoding a novel protein, exchange factor for ARF6 (EFA6), which contains Sec7 and pleckstrin homology domains. EFA6 promotes efficient guanine nucleotide exchange on ARF6 and is distinct from the ARNO family of ARF1 exchange factors. The protein localizes to a dense matrix on the cytoplasmic face of plasma membrane invaginations, induced on its expression. We show that EFA6 regulates endosomal membrane recycling and promotes the redistribution of transferrin receptors to the cell surface. Furthermore, expression of EFA6 induces actin-based membrane ruffles that are inhibited by co-expression of dominant-inhibitory mutant forms of ARF6 or Rac1. Our results demonstrate that by catalyzing nucleotide exchange on ARF6 at the plasma membrane and by regulating Rac1 activation, EFA6 coordinates endocytosis with cytoskeletal rearrangements.  (+info)

Structural basis for the inhibitory effect of brefeldin A on guanine nucleotide-exchange proteins for ADP-ribosylation factors. (6/1111)

Protein secretion through the endoplasmic reticulum and Golgi vesicular trafficking system is initiated by the binding of ADP-ribosylation factors (ARFs) to donor membranes, leading to recruitment of coatomer, bud formation, and eventual vesicle release. ARFs are approximately 20-kDa GTPases that are active with bound GTP and inactive with GDP bound. Conversion of ARF-GDP to ARF-GTP is regulated by guanine nucleotide-exchange proteins. All known ARF guanine nucleotide-exchange proteins contain a Sec7 domain of approximately 200 amino acids that includes the active site and fall into two classes that differ in molecular size and susceptibility to inhibition by the fungal metabolite brefeldin A (BFA). To determine the structural basis of BFA sensitivity, chimeric molecules were constructed by using sequences from the Sec7 domains of BFA-sensitive yeast Sec7 protein (ySec7d) and the insensitive human cytohesin-1 (C-1Sec7). Based on BFA inhibition of the activities of these molecules with recombinant yeast ARF2 as substrate, the Asp965-Met975 sequence in ySec7d was shown to be responsible for BFA sensitivity. A C-1Sec7 mutant in which Ser199, Asn204, and Pro209 were replaced with the corresponding ySec7d amino acids, Asp965, Gln970, and Met975, exhibited BFA sensitivity similar to that of recombinant ySec7d (rySec7d). Single replacement in C-1Sec7 of Ser199 or Pro209 resulted in partial inhibition by BFA, whereas replacement of Gln970 in ySec7d with Asn (as found in C-1Sec7) had no effect. As predicted, the double C-1Sec7 mutant with S199D and P209M was BFA-sensitive, demonstrating that Asp965 and Met975 in ySec7d are major molecular determinants of BFA sensitivity.  (+info)

Characterization of the regulation of phospholipase D activity in the detergent-insoluble fraction of HL60 cells by protein kinase C and small G-proteins. (7/1111)

Phospholipase D (PLD) activity has been shown to be GTP-dependent both in vivo and in vitro. One protein that confers GTP sensitivity to PLD activity in vitro is the low-molecular-mass G-protein ADP-ribosylation factor (Arf). However, members of the Rho family and protein kinase C (PKC) have also been reported to activate PLD in various cell systems. We have characterized the stimulation of PLD in HL60 cell membranes by these proteins. The results demonstrate that a considerable proportion of HL60 PLD activity is located in a detergent-insoluble fraction of the cell membrane that is unlikely to be a caveolae-like domain, but is probably cytoskeletal. This PLD activity required the presence of Arf1, a Rho-family member and PKC for efficient catalysis of the lipid substrate, suggesting that the activity represents PLD1. We show that recombinant human PLD1b is regulated in a similar manner to HL60-membrane PLD, and that PKCalpha and PKCdelta are equally effective PLD activators. Therefore maximum PLD activity requires Arf, a Rho-family member and PKC, emphasizing the high degree of regulation of this enzyme.  (+info)

The ADP-ribosylation factor (ARF)-related GTPase ARF-related protein binds to the ARF-specific guanine nucleotide exchange factor cytohesin and inhibits the ARF-dependent activation of phospholipase D. (8/1111)

ADP-ribosylation factor-related protein (ARP) is a membrane-associated GTPase with remote similarity to the family of ADP-ribosylation factors (ARF). In a yeast two-hybrid screen designed to identify proteins interacting with ARP, we isolated a partial cDNA of the ARF-specific guanine nucleotide exchange factor mSec7-1/cytohesin encoding its N terminus and most of the Sec7 domain (codons 1-200). ARP and ARP-Q79L (GTPase-negative ARP) exhibited a higher affinity to mSec7-1-(1-200) than ARP-T31N (nucleotide exchange-defective ARP) in the two-hybrid assay. Similarly, full-length [35S]mSec7-1/cytohesin was specifically adsorbed to glutathione-Sepharose loaded with glutathione S-transferase (GST)-ARP-Q79L, GST-ARP, or GST-ARP-T31N, the latter exhibiting the lowest binding affinity. Overexpression of ARP-Q79L, but not of ARP-T31N, in COS-7 cells reduced the fluorescence from co-expressed green fluorescent protein fused with mSec7-1/cytohesin or mSec7-2/ARNO in plasma membranes as detected by deconvolution microscopy. Recombinant ARP and ARP-Q79L, but not ARP-T31N, inhibited the phospholipase D (PLD) activity stimulated by mSec7-2/ARNO and ARF in a system of isolated membranes. Furthermore, transfection of HEK-293 cells with ARP or ARP-Q79L, but not ARP-T31N, inhibited the muscarinic acetylcholine receptor-3 induced PLD stimulation and translocation of ARF from cytosol to membranes. These data suggest that the GTP-bound form of ARP specifically binds mSec7-1/cytohesin, and that ARP may be involved in a pathway inhibiting the ARF-controlled activity of PLD.  (+info)

Looking for online definition of ADP-ribosylation factor-like tumour-suppressor protein 1 in the Medical Dictionary? ADP-ribosylation factor-like tumour-suppressor protein 1 explanation free. What is ADP-ribosylation factor-like tumour-suppressor protein 1? Meaning of ADP-ribosylation factor-like tumour-suppressor protein 1 medical term. What does ADP-ribosylation factor-like tumour-suppressor protein 1 mean?
Buy ARFGAP1 recombinant protein, ADP-ribosylation factor GTPase-activating protein 1 (ARFGAP1) Recombinant Protein-NP_001268411.1 (MBS1301288) product datasheet at MyBioSource, Recombinant Proteins
Myc-DDK-tagged ORF clone of Homo sapiens ADP-ribosylation factor-like 5C (ARL5C) as transfection-ready DNA - 10 µg - OriGene - cdna clones
Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). Required for normal cytokinesis and cilia signaling. Requires assistance from GTPase-activating proteins (GAPs) like RP2 and PDE6D, in order to cycle between inactive GDP-bound and active GTP-bound forms. Required for targeting proteins such as NPHP3 to the ciliary membrane by releasing myristoylated NPHP3 from UNC119B cargo adapter into the cilium. Does not act as an allosteric activator of the cholera toxin catalytic subunit ...
Guanine nucleotide exchange factor for Arf GTPases, stimulating the nucleotide exchange from the GDP-bound to the GTP-bound form. Catalyzes both the GDP release by and the GTP binding to ARF2. Has no exhange activity on Rab GTPases. Involved in vesicular transport.
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The KOMP Repository is located at the University of California Davis and Childrens Hospital Oakland Research Institute. Question? Comments? For Mice, Cells, and germplasm please contact us at [email protected], US 1-888-KOMP-MICE or International +1-530-752-KOMP, or for vectors [email protected] or +1-510-450-7917 ...
TY - JOUR. T1 - ADP ribosylation factor 6 regulates neuronal migration in the developing cerebral cortex through FIP3/arfophilin-1-dependent endosomal trafficking of N-cadherin. AU - Hara, Yoshinobu. AU - Fukaya, Masahiro. AU - Hayashi, Kanehiro. AU - Kawauchi, Takeshi. AU - Nakajima, Kazunori. AU - Sakagami, Hiroyuki. PY - 2016. Y1 - 2016. N2 - During neural development, endosomal trafficking controls cell shape and motility through the polarized transport of membrane proteins related to cellcell and cellextracellular matrix interactions. ADP ribosylation factor 6 (Arf6) is a critical small GTPase that regulates membrane trafficking between the plasma membrane and endosomes. We herein demonstrated that the knockdown of endogenous Arf6 in mouse cerebral cortices led to impaired neuronal migration in the intermediate zone and cytoplasmic retention of N-cadherin and syntaxin12 in migrating neurons. Rescue experiments with separation-of-function Arf6 mutants identified Rab11 familyinteracting ...
Ver más] The small GTP-binding protein ADP-ribosylation factor 1 (ARF1) is an essential component of the molecular machinery that catalyzes the formation of membranebound transport intermediates. By using an in vitro assay that reproduces recruitment of cytosolic proteins onto purified, high salt-washed Golgi membranes, we have analyzed the role of cAMP-dependent protein kinase A (PKA) on ARF1 incorporation. Addition to this assay of either pure catalytic subunits of PKA (C-PKA) or cAMP increased ARF1 binding. By contrast, ARF1 association was inhibited following C-PKA inactivation with either PKA inhibitory peptide or RIIa as well as after cytosol depletion of C-PKA. C-PKA also stimulated recruitment and activation of a recombinant form of human ARF1 in the absence of additional cytosolic components. The binding step could be dissociated from the activation reaction and found to be independent of guanine nucleotides and saturable. This step was stimulated by C-PKA in an ATP-dependent manner. ...
ADP-ribosylation factors (ARFs) are approximately 20-kDa guanine nucleotide-binding proteins recognized as critical components in intracellular vesicular transport and phospholipase D activation. Both guanine nucleotide-exchange proteins and GTPase-activating proteins (GAPs) for ARFs have been cloned recently. A zinc finger motif near the amino terminus of the ARF1 GAP was required for stimulation of GTP hydrolysis. ARD1 is an ARF family member that differs from other ARFs by the presence of a 46-kDa amino-terminal extension. We had reported that the ARF domain of ARD1 binds specifically GDP and GTP and that the amino-terminal extension acts as a GAP for the ARF domain of ARD1 but not for ARF proteins. The GAP domain of ARD1, synthesized in Escherichia coli, stimulated hydrolysis of GTP bound to the ARF domain of ARD1. Using ARD1 truncations, it appears that amino acids 101-190 are critical for GAP activity, whereas residues 190-333 are involved in physical interaction between the two domains of ...
ADP-ribosylation factor-like protein 4A (ARL4A) is a developmentally regulated member of the ARF/ARL GTPase family. The primary structure of ARL4A is very similar to that of other ARF/ARL molecules, but its function remains unclear. The trans-Golgi network golgin GCC185 is required for maintenance of Golgi structure and distinct endosome-to-Golgi transport. We show here that GCC185 acts as a new effector for ARL4 to modulate Golgi organization. ARL4A directly interacts with GCC185 in a GTP-dependent manner. Sub-coiled-coil regions of the CC2 domain of GCC185 are required for the interaction between GCC185 and ARL4A. Depletion of ARL4A reproduces the GCC185-depleted phenotype, causing fragmentation of the Golgi compartment and defects in endosome-to-Golgi transport. GCC185 and ARL4A localize to the Golgi independently of each other. Deletion of the ARL4A-interacting region of GCC185 results in inability to maintain Golgi structure. Depletion of ARL4A impairs the interaction between GCC185 and ...
TY - JOUR. T1 - Active Arf6 recruits ARNO/cytohesin GEFs to the PM by binding their PH domains. AU - Cohen, Lee Ann. AU - Honda, Akira. AU - Várnai, P.. AU - Brown, Fraser D.. AU - Balla, Tamas. AU - Donaldson, Julie G.. PY - 2007/6. Y1 - 2007/6. N2 - ARNO is a soluble guanine nucleotide exchange factor (GEF) for the Arf family of GTPases. Although in biochemical assays ARNO prefers Arf1 over Arf6 as a substrate, its localization in cells at the plasma membrane (PM) suggests an interaction with Arf6. In this study, we found that ARNO activated Arf1 in HeLa and COS-7 cells resulting in the recruitment of Arf1 on to dynamic PM ruffles. By contrast, Arf6 was activated less by ARNO than EFA6, a canonical Arf6 GEF. Remarkably, Arf6 in its GTP-bound form recruited ARNO to the PM and the two proteins could be immunoprecipitated. ARNO binding to Arf6 was not mediated through the catalytic Sec7 domain, but via the pleckstrin homology (PH) domain. Active Arf6 also bound the PH domain of Grp1, another ...
This gene encodes a member of an ADP-ribosylation factor GTPase-activating protein family involved in membrane trafficking and cytoskeleton dynamics. This gene functions as a direct regulator of the adaptor-related protein complex 3 on endosomes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011 ...
ARF1 - ARF1 (untagged)-Human ADP-ribosylation factor 1 (ARF1), transcript variant 4 available for purchase from OriGene - Your Gene Company.
We have demonstrated previously the potent activation of PLD by the chemokine IL-8 in T lymphocytes (18). We have now extended our findings to include the C-C chemokine RANTES in demonstrating that in the Jurkat T cell line, the activation of this enzyme occurs at subnanomolar concentrations and is dependent on the activation of small GTP-binding protein cofactors. RANTES-induced PLD activation is consistently maximal at 1 nM, a concentration corresponding to the optimal chemotaxis-inducing dose in normal T lymphocytes. Interestingly, PLD activation in T lymphocytes and Jurkat T cells appears to be an important biologic consequence of chemokine action and more readily measurable (at nanomolar concentrations) than readouts of receptor activation such as calcium flux. It was also apparent that RANTES is the only chemokine tested to date (RANTES, MIP-1α, MIP-1β, MCP-1, MCP-3, lymphotactin) that induces as robust a response as seen in this study, although the others listed were capable of low ...
Arl1 (ARF like protein1) is a poorly understood member of ARF family small GTPases. This thesis presents an original characterization of Arl1 and its effectors. Arl1 was localized to the tans Golgi under EM. Over expression of guanine nucleotide mutants of Arl1 dramatically affects the structure and function of Golgi apparatus. Arl1-GTP was found to interact with GRIP domain of Golgins (Golgin-97, Golgin-245, GCC1 and KIAA0336). The interaction was dependent on the conserved amino acids on both switch II region of Arl1 and the GRIP domain. Collectively, the research presented in this thesis reveals Arl1 is a new regulator of Golgi structure and function and one mechanism of Arl1a??s function is that it recruits and regulates its effectors a?? GRIP domain Golgins to Golgi ...
Human gamma-adaptin-related protein 2 ELISA Kit;Human golgi-localized, gamma ear-containing, ARF-binding protein 2 ELISA Kit;Human VHS domain and ear domain of gamma-adaptin ELISA Kit;Human VEAR ELISA Kit;Human KIAA1080 ELISA Kit;Human golgi associated, gamma adaptin ear containing, ARF binding protein 2 ELISA Kit;Human ADP-ribosylation factor-binding protein GGA2 ELISA Kit;Human VHS domain and ear domain-containing protein ELISA Kit ...
Abbreviations: ABC, ATP-binding cassette transporter; ANGPTL3, angiopoietin-like 3; apo, apolipoprotein; ARL7, ADP-ribosylation factor-like 7; CAD, coronary artery disease; CXCR4, CXC receptor 4; EC, endothelial cell; FOXA2, forkhead box A2; GPAM, glycerol-3-phosphate acyltransferase 1; HDL, high-density lipoprotein; HDL-C, HDL-cholesterol; HMGCR, 3-hydroxy-3-methylglutaryl-CoA reductase; IL, interleukin; LDL, low-density lipoprotein; LDLR, LDL receptor; LNA, locked nucleic acid; LOX-1, oxidized LDL receptor 1; LPL, lipoprotein lipase; LPS, lipopolysaccharide; LXR, liver X receptor; Mct1, monocarboxylate transporter 1; MI, myocardial infarction; miRNA, microRNA; Mtpn, myotrophin; NFAT, nuclear factor of activated T-cells; NF-κB, nuclear factor κB; p27Kip1, cyclin-dependent kinase inhibitor 1B; p57Kip2, cyclin-dependent kinase inhibitor 1C; oxLDL, oxidized LDL; PEPCK, phosphoenolpyruvate carboxykinase; PI3K, phosphoinositide 3-kinase; PPAR, peroxisome proliferator-activated receptor; pre-miRNA, ...
The small GTP-binding proteins of the ARF family play a central role in membrane dynamics and protein transport in eukaryotic cells. The GEFs that catalyze GDP/GTP exchange on ARF are of critical importance to ARF function, as they determine when and where ARF proteins will be activated within the cell (reviewed by Jackson and Casanova, 2000). We present here an in vivo characterization of the Gea1p and Gea2p ARF GEFs in the secretory pathway of S. cerevisiae. The Gea1p and Gea2p proteins are members of a subfamily of ARF GEFs with members in plants and animals, as well as in yeast ( Claude et al., 1999; Steinmann et al., 1999). This subfamily is distinct from that of Sec7p and its mammalian orthologues BIG1 and BIG2 ( Mansour et al., 1999; Morinaga et al., 1997; Togawa et al., 1999).. The Gea1p and Gea2p proteins are functionally redundant, but at least one is necessary for viability in yeast. We have generated three temperature-sensitive gea mutants and have examined their phenotypes. The ...
D. Jones, B. Bax, S. Cockcroft; ADP-ribosylation factor GTPases in signal transduction and membrane traffic: independent functions?. Biochem Soc Trans 1 August 1999; 27 (4): 642-647. doi: https://doi.org/10.1042/bst0270642. Download citation file:. ...
Top performende anti-Ratte (Rattus) ADP-Ribosylation Factor 1 Antikörper für Immunofluorescence (Paraffin-embedded Sections) (IF (p)) vergleichen & kaufen.
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the GIT protein family, which interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. GIT proteins traffic between cytoplasmic complexes, focal adhesions, and the cell periphery, and interact with Pak interacting exchange factor beta (PIX) to form large oligomeric complexes that transiently recruit other proteins. GIT proteins regulate cytoskeletal dynamics and participate in receptor internalization and membrane trafficking. This gene has been shown to repress lamellipodial extension and focal adhesion turnover, and is thought to regulate cell motility. This gene undergoes extensive alternative splicing to generate multiple isoforms, but the full-length nature of some of these variants has not been determined. The various isoforms have functional differences, with respect to ARF GAP activity ...
Rafiq et al. demonstrate that the small G protein ARF1 and its activator, cytohesin 2 (ARNO), are required for podosome formation in macrophage-like cells and fibroblasts. Inhibition of ARNO-ARF1 signaling results in increased RhoA activity and disassembly of podosomes in a myosin-IIA-dependent fashion. In fibroblasts that normally do not form podosomes, constitutively active ARF1 induces actin-rich puncta associated with sites of matrix degradation, putative precursors of podosomes. ...
Cell migration is an orchestrated and highly coordinated multi-step process that is central to the development and maintenance of multicellular organisms. Dysregulated migration however, is associated with pathological states such as tumor formation and metastasis; thus a clear understanding of the molecular mechanisms that drive this process is critical to the development of counteracting therapeutics. Cell migration and adhesion-dependent cell spreading share a number of features. For example, both processes rely on the activation of mechanisms for the coordinated spatial and temporal assembly/disassembly of focal adhesions, as well as mechanisms controlling actin rearrangements and directed vesicular trafficking. Actin remodeling and vesicular trafficking events are in turn, implicated functions of a variety of small GTPases of the Ras superfamily, which include the Rho and Arf subfamilies. Thus towards efforts of further characterizing the molecular pathways that drive cell spreading, I pursued aims
Two closely related human Arls, Arl8a and Arl8b, were found to localise to lysosomes in mammalian cells. conventionally, membrane binding of Arf and Arl proteins is mediated by both an N-terminal myristoyl group and an N-terminal amphipathic helix that are inserted into the lipid bilayer upon activation of the GTPase. Arl8 GPTases lack myristolylation sites, and examination of the N-terminus of Arl8b revealed that it contains an acetyl group instead, and this acetylated methionine is necessary for its lysosomal location. Lysosomes of cells overexpressing Arl8b move more frequently, suggesting a role for Arl8a and Arl8b as positive regulators of lysosomal transport. Arl4a, Arl4c and Arl4d are very similar in sequence and were found to act in a pathway upstream of Arf6, Arf6 is a regulator of key processes at the plasma membrane, such as endocytosis, actin dynamics and cell adhesion. One of the major activators of Arf6 is the exchange factor ARNO (Arf nucleotide binding site opener). In order to ...
... Arf 6 Activation Assay Kit bases on the configuration-specific anti-Arf 6-GTP monoclonal antibody to measure the active Arf 6-GTP levels, either from cell extracts or from in vitro GTPγS loading Arf 6 activation assays. Briefly, anti-active Arf 6 mouse monoclonal antibody will be incubated with cell lysates containing Arf 6-GTP. The bound active Arf 6 will then be pulled down by protein A/G agarose. The precipitated active Arf 6 will be detected by immunoblot analysis using an anti-Arf 6 rabbit polyclonal antibody, respectively. ...
COPⅠ囊泡:最初研究者利用三磷酸鳥苷(GTP)衍生物GTPγS(一種富含高爾基體膜的細胞質與抗水解的GTP衍生物)共培養時,發現高爾基體池之間存在一種囊泡轉運結構[9](後來在真核細胞中也證實此結構的存在[10])。除了脂質成分外,參與此囊泡形成的成份還有7種外被體蛋白(即外被體α、β、β′、γ、δ、ε、ζ)。這些外被體蛋白相互作用形成的復合物就是COPⅠ囊泡[11][12]。亞單位α、β′、ε在結構上與網格蛋白及COPⅡ囊泡的外層組分具有較高的一致性,形成復合物的內層組分稱為B亞復合物(主要負責與靶蛋白結合),而亞單位β、γ、δ、ζ 與網格蛋白及COPⅡ囊泡的內層組分相似,形成復合物的內層組分稱為F亞復合物,該亞復合物主要負責與靶蛋白結合,並且直接與COPⅠ囊泡形成的招募者ADP核糖基化因子(英语:ADP ribosylation factor)(ADP ribosylation ...
Arf-like protein 1 (Arl1) is a member of the Arf family of regulatory GTPases, within the Ras superfamily of GTPases, and with highly conserved orthologs throughout eukaryotes. Arl1 is essential for early embryonic development in Drosophila and in Caenorhabditis elegans. Arl1 is most similar in primary sequence, cellular location, and function (regulation of membrane traffic) to Arf1-6 and even shares several common binding partners. In addition to its function in membrane traffic at the Golgi/trans-Golgi network, there are reports indicating a possible role for Arl1 in ion homeostasis in yeast. ...
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy ...
Other than a role for Ca2+ store depletion, the molecular mechanisms that regulate antigen-stimulated Ca2+ influx into mast cells are not well-understood. The observation that CT dramatically enhances 45Ca2+ influx into RBL-2H3 cells suggests that this reagent might be a useful tool to study the Ca2+ entry pathway (Narasimhan et al., 1988). That CT amplifies both antigen-evoked ICRAC and 45Ca2+ influx to a similar extent bolsters the idea that CRAC channels are a major pathway for FcεRI-mediated Ca2+ uptake into RBL-2H3 mast cells (Zhang and McCloskey, 1995).. Two hypotheses to explain the effect of CT on 45Ca2+ influx are immediately testable by patch clamping. First, it is possible that CT activates Cl− or K+ channels, and thereby increases the electrical force propelling Ca2+ entry. This indirect mechanism cannot explain the enhancement of Ca2+ influx currents that we observed, because voltage-clamp measurements eliminate any difference in membrane potential between control and CT-treated ...
CONTACT: [email protected] GENBANK UPDATE: 22 July 1997 28 unique yeast ORFs ORF NAME LOCUS PRODUCT/DESCRIPTION -------- ----- ---------------------------------------- YFL039C ACT1 Actin YDL192W ARF1 ADP-ribosylation factor YDL137W ARF2 ADP-ribosylation factor 2 YJR121W ATP2 F(1)F(0)-ATPase complex beta subunit, mitochondrial YER177W BMH1 Homolog of mammalian 14-3-3 proteins YLR229C CDC42 member of the Rho subfamily of Ras-like proteins YER133W GLC7 protein phosphatase type I YLR293C GSP1 GTP-binding protein YOR185C GSP2 GTP-binding protein YBR009C HHF1 Histone H4 (HHF1 and HHF2 code for identical proteins) YNL030W HHF2 Histone H4 (HHF1 and HHF2 code for identical proteins) YBR010W HHT1 Histone H3 (HHT1 and HHT2 code for identical proteins) YNL031C HHT2 Histone H3 (HHT1 and HHT2 code for identical proteins) YDR224C HTB1 Histone H2B (HTB1 and HTB2 code for nearly identical proteins) YBL002W HTB2 Histone H2B (HTB1 and HTB2 code for nearly identical proteins) YBL087C RPL17A Ribosomal protein ...
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Buy our Recombinant Human ARL15 protein. Ab113118 is a full length protein produced in Escherichia coli and has been validated in SDS-PAGE, MS. Abcam provides…
Recombinant Human ARL3 protein is an Escherichia coli Full length protein 1 to 182 aa range, | 95% purity and validated in SDS-PAGE, MS.
Complete information for ARF6 gene (Protein Coding), ADP Ribosylation Factor 6, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Complete information for ARL6IP6 gene (Protein Coding), ADP Ribosylation Factor Like GTPase 6 Interacting Protein 6, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
ARFGAP3 antibody, C-term (ADP-ribosylation factor GTPase activating protein 3) for WB. Anti-ARFGAP3 pAb (GTX89988) is tested in Mouse samples. 100% Ab-Assurance.
Homo sapiens golgi associated, gamma adaptin ear containing, ARF binding protein 1 (GGA1), transcript variant 2, mRNA. (H00026088-R01) - Products - Abnova
I want to BRAG big time about Becky and Sheila for great Tourism Currents track at Blogworld LA. Their positive energy comes through in every tweet and photos people are sharing.. I also want to brag again on my girl Jeanne Robertson. She was in 5 cities in 5 days. She is remarkable. btw, at 68 she is busier and more popular than ever. She credits modern tools like Facebook and YouTube for her new career. She has been a professional speaker for 48 years.. Gee, when Becky said "you are in charge of the brag basket" she probably didnt mean just me. What wonderful things have yall been doing this week?. ...
ADP-ribosylation factor, GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the ...
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
ARFIP2 antibody [N1C2] (ADP-ribosylation factor interacting protein 2) for IHC-P, WB. Anti-ARFIP2 pAb (GTX104241) is tested in Human samples. 100% Ab-Assurance.
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
BFA induces the ADP-ribosylation of BARS-50 and GAPDH in permeabilized cells. (A) RBL cells were permeabilized with 3 U/ml SLO and exposed to 10 μg/ml BFA
FUNCTION: Guanine nucleotide-exchange factor (GEF) required for the formation or budding of transport vesicles from the ER. This function involves the cytoplasmic domain of the protein, which is thought to interact with the small GTP-binding protein SAR1. Required for autophagy. MISCELLANEOUS: In the process of transport, SEC12 itself may migrate to the Golgi apparatus and function in subsequent transport events. MISCELLANEOUS: Present with 6160 molecules/cell in log phase SD medium ...
Arginine adenosine-5′-diphosphoribosylation (ADP-ribosylation) is an enzyme-catalyzed potentially reversible posttranslational changes where the ADP-ribose moiety is transferred from NAD+ towards the guanidino CHR2797 moiety of arginine. proteins with binding companions e.g. toxin-catalyzed ADP-ribosylation of actin at R177 blocks actin polymerization sterically. In case there is the nucleotide-gated P2X7 ion route ADP-ribosylation at R125 near the ligand-binding site causes route Rabbit Polyclonal to SF3B3. gating. Arginine-specific ADP-ribosyltransferases (ARTs) bring a quality R-S-EXE theme that distinguishes these enzymes from structurally related enzymes which catalyze ADP-ribosylation of additional amino acid part chains DNA or little substances. Arginine-specific ADP-ribosylation could be inhibited by little molecule arginine analogues such as for example CHR2797 agmatine or meta-iodobenzylguanidine (MIBG) which themselves can serve as focuses on for arginine-specific ARTs. ...
Summary Arl (Arf-like) proteins, GTP-binding proteins of the Ras superfamily, are molecular switches that cycle between a GDP-bound inactive and GTP-bound active state. There are 16 members of the Arl subfamily in the human genome whose basic mechanistic function is unknown. The interactome of Arl2/3 includes proteins involved in retinopathies and other ciliary diseases such as Leber¿s Congenital Amaurosis (LCA) and kidney diseases such as nephronophthisis. Arl6 has been found mutated in Bardet Biedl Syndrome, another pleiotropic ciliary disease. In the proposed interdisciplinary project I want to explore the function of the protein network of Arl2/3 and Arl6 by a combination of biochemical, biophysical and structural methods and use the knowledge obtained to probe their function in live cells. As with other subfamily proteins of the Ras superfamily which have been found to mediate similar biological functions I want to derive a basic understanding of the function of Arl proteins and how it ...
ADP ribosylation factor 1 (Arf1). Arl1 subfamily. Arl1 (Arf-like 1) localizes to the Golgi complex, where it is believed to recruit effector proteins to the trans-Golgi network. Like most members of the Arf family, Arl1 is myristoylated at its N-terminal helix and mutation of the myristoylation site disrupts Golgi targeting. In humans, the Golgi-localized proteins golgin-97 and golgin-245 have been identified as Arl1 effectors. Golgins are large coiled-coil proteins found in the Golgi, and these golgins contain a C-terminal GRIP domain, which is the site of Arl1 binding. Additional Arl1 effectors include the GARP (Golgi-associated retrograde protein)/VFT (Vps53) vesicle-tethering complex and Arfaptin 2. Arl1 is not required for exocytosis, but appears necessary for trafficking from the endosomes to the Golgi. In Drosophila zygotes, mutation of Arl1 is lethal, and in the host-bloodstream form of Trypanosoma brucei, Arl1 is essential for viability. ...
ADP-ribosylation factor 1 is a protein that in humans is encoded by the ARF1 gene. ADP-ribosylation factor 1 (ARF1) is a member ... Frank S, Upender S, Hansen SH, Casanova JE (1998). "ARNO is a guanine nucleotide exchange factor for ADP-ribosylation factor 6 ... ARF1 ADP-ribosylation factor 1". Mitchell R, Robertson DN, Holland PJ, Collins D, Lutz EM, Johnson MS (September 2003). "ADP- ... 1991). "ADP-ribosylation factor is a subunit of the coat of Golgi-derived COP-coated vesicles: a novel role for a GTP-binding ...
Activation of the purified enzymes by phosphatidylinositol 4,5-bisphosphate, ADP-ribosylation factor, and Rho family monomeric ... ADP-ribosylation factor regulates hPLD2". J. Biol. Chem. 273 (21): 12846-52. doi:10.1074/jbc.273.21.12846. PMID 9582313. Kim JH ... "Human ADP-ribosylation factor-activated phosphatidylcholine-specific phospholipase D defines a new and highly conserved gene ... "Activation of phospholipase D1 by direct interaction with ADP-ribosylation factor 1 and RalA". FEBS Lett. 430 (3): 231-5. doi: ...
ADP-ribosylation factor-like protein 13B (ARL13B), also known as ADP-ribosylation factor-like protein 2-like 1, is a protein ... This gene encodes a member of the ADP-ribosylation factor-like family. The encoded protein is a small GTPase that contains both ... ADP-ribosylation factor-like 13B". Cantagrel V, Silhavy JL, Bielas SL, Swistun D, Marsh SE, Bertrand JY, Audollent S, Attié- ...
ADP-ribosylation factor-like protein 8A is a protein that in humans is encoded by the ARL8A gene. GRCh38: Ensembl release 89: ... "Entrez Gene: ARL8A ADP-ribosylation factor-like 8A". Human ARL8A genome location and ARL8A gene details page in the UCSC Genome ...
ADP-ribosylation factor-like protein 6 is a protein that in humans is encoded by the ARL6 gene. The protein encoded by this ... "Entrez Gene: ARL6 ADP-ribosylation factor-like 6". GeneReviews/NIH/NCBI/UW entry on Bardet-Biedl Syndrome Human ARL6 genome ... 1999). "ADP-ribosylation factor (ARF)-like 4, 6, and 7 represent a subgroup of the ARF family characterization by rapid ... 1999). "A novel ADP-ribosylation like factor (ARL-6), interacts with the protein-conducting channel SEC61beta subunit". FEBS ...
ADP-ribosylation factor-like protein 2 is a protein that in humans is encoded by the ARL2 gene. The ADP-ribosylation factor ( ... "Entrez Gene: ARL2 ADP-ribosylation factor-like 2". Kobayashi A, Kubota S, Mori N, McLaren MJ, Inana G (January 2003). " ... Beghin A, Honore S, Messana C, Matera EL, Aim J, Burlinchon S, Braguer D, Dumontet C (2007). "ADP ribosylation factor like 2 ( ... Bhamidipati A, Lewis SA, Cowan NJ (May 2000). "ADP ribosylation factor-like protein 2 (Arl2) regulates the interaction of ...
ADP-ribosylation factor regulates hPLD2". J Biol Chem. 273 (21): 12846-52. doi:10.1074/jbc.273.21.12846. PMID 9582313. "Entrez ... "Cardiac phospholipase D2 localizes to sarcolemmal membranes and is inhibited by alpha-actinin in an ADP-ribosylation factor- ... "Cardiac phospholipase D2 localizes to sarcolemmal membranes and is inhibited by alpha-actinin in an ADP-ribosylation factor- ... "The direct interaction of phospholipase C-gamma 1 with phospholipase D2 is important for epidermal growth factor signaling". J ...
BFA blocks the activation of some ADP-ribosylation factors (ARFs). ARFs are small GTPases which regulate vesicular trafficking ... BFA inhibits the function of several guanine nucleotide exchange factors (GEFs) that mediate GTP-binding of ARFs. Treatment of ...
Premont RT, Claing A, Vitale N, Perry SJ, Lefkowitz RJ (July 2000). "The GIT family of ADP-ribosylation factor GTPase- ... a G protein-coupled receptor kinase-associated ADP ribosylation factor GTPase-activating protein". Proc. Natl. Acad. Sci. U.S.A ... receptor kinase 2 in a signaling unit that regulates p42/p44 mitogen-activated protein kinase by epidermal growth factor". J. ...
Premont RT, Claing A, Vitale N, Perry SJ, Lefkowitz RJ (August 2000). "The GIT family of ADP-ribosylation factor GTPase- ... a G protein-coupled receptor kinase-associated ADP ribosylation factor GTPase-activating protein". Proc Natl Acad Sci U S A. 95 ... Zhao ZS, Manser E, Loo TH, Lim L (September 2000). "Coupling of PAK-interacting exchange factor PIX to GIT1 promotes focal ... Zhao ZS, Manser E, Loo TH, Lim L (2000). "Coupling of PAK-interacting exchange factor PIX to GIT1 promotes focal complex ...
ADP-ribosylation factor 6 (ARF6) is a member of the ADP ribosylation factor family of GTP-binding proteins. ARF6 has a variety ... Frank S, Upender S, Hansen SH, Casanova JE (1998). "ARNO is a guanine nucleotide exchange factor for ADP-ribosylation factor 6 ... "ADP-ribosylation factor 6 as a target of guanine nucleotide exchange factor GRP1". J. Biol. Chem. 274 (38): 27099-104. doi: ... Amor JC, Harrison DH, Kahn RA, Ringe D (1995). "Structure of the human ADP-ribosylation factor 1 complexed with GDP". Nature. ...
ADP-ribosylation factor 4 is a protein that in humans is encoded by the ARF4 gene. ADP-ribosylation factor 4 (ARF4) is a member ... ARF4 ADP-ribosylation factor 4". Kim SW, Hayashi M, Lo JF, Yang Y, Yoo JS, Lee JD (Jan 2003). "ADP-ribosylation factor 4 small ... "ADP-ribosylation factor 4 small GTPase mediates epidermal growth factor receptor-dependent phospholipase D2 activation". J. ... Kahn RA, Kern FG, Clark J, Gelmann EP, Rulka C (1991). "Human ADP-ribosylation factors. A functionally conserved family of GTP- ...
ADP-ribosylation factor-like 15 is a protein in humans that is encoded by the ARL15 gene. GRCh38: Ensembl release 89: ... ADP-ribosylation factor-like 15". Retrieved 2013-03-16. ...
ADP-ribosylation factor 3 is a protein that in humans is encoded by the ARF3 gene. ADP-ribosylation factor 3 (ARF3) is a member ... "Entrez Gene: ARF3 ADP-ribosylation factor 3". Kanoh H, Williger BT, Exton JH (February 1997). "Arfaptin 1, a putative cytosolic ... Williger BT, Provost JJ, Ho WT, Milstine J, Exton JH (July 1999). "Arfaptin 1 forms a complex with ADP-ribosylation factor and ... Lee CM, Haun RS, Tsai SC, Moss J, Vaughan M (1992). "Characterization of the human gene encoding ADP-ribosylation factor 1, a ...
"Entrez Gene: ARL6IP5 ADP-ribosylation-like factor 6 interacting protein 5". Human ARL6IP5 genome location and ARL6IP5 gene ... 1999). "A novel ADP-ribosylation like factor (ARL-6), interacts with the protein-conducting channel SEC61beta subunit". FEBS ...
ADP-ribosylation factor-like protein 6-interacting protein 1 is a protein that in humans is encoded by the ARL6IP1 gene. GRCh38 ... ARL6IP1 ADP-ribosylation factor-like 6 interacting protein 1". Human ARL6IP1 genome location and ARL6IP1 gene details page in ... "A novel ADP-ribosylation like factor (ARL-6), interacts with the protein-conducting channel SEC61beta subunit". FEBS Lett. 459 ... ADP-ribosylation-like factor-6 interacting protein (ARL6)". Genomics. 68 (3): 351-4. doi:10.1006/geno.2000.6278. PMID 10995579 ...
"Entrez Gene: ARFIP1 ADP-ribosylation factor interacting protein 1 (arfaptin 1)". Tsai SC, Adamik R, Hong JX, et al. (1998). " ... Williger BT, Provost JJ, Ho WT, Milstine J, Exton JH (Aug 1999). "Arfaptin 1 forms a complex with ADP-ribosylation factor and ... Ho WT, Exton JH, Williger BT (2003). "Arfaptin 1 inhibits ADP-ribosylation factor-dependent matrix metalloproteinase-9 ... a putative cytosolic target protein of ADP-ribosylation factor, is recruited to Golgi membranes". J Biol Chem. 272 (9): 5421-9 ...
ADP-ribosylation factor-like protein 4A is a protein that in humans is encoded by the ARL4A gene. ADP-ribosylation factor-like ... Jacobs S, Schilf C, Fliegert F, Koling S, Weber Y, Schürmann A, Joost HG (1999). "ADP-ribosylation factor (ARF)-like 4, 6, and ... ARL4A ADP-ribosylation factor-like 4A". Lin CY, Huang PH, Liao WL, Cheng HJ, Huang CF, Kuo JC, Patton WA, Massenburg D, Moss J ... 4A is a member of the ADP-ribosylation factor family of GTP-binding proteins. ARL4A is similar to ARL4C and ARL4D and each has ...
ADP-ribosylation factor-like protein 4D is a protein that in humans is encoded by the ARL4D gene. ADP-ribosylation factor 4D is ... "Entrez Gene: ARL4D ADP-ribosylation factor-like 4D". "DEXA data for Arl4d". Wellcome Trust Sanger Institute. "Radiography data ... Jacobs S, Schilf C, Fliegert F, Koling S, Weber Y, Schürmann A, Joost HG (Aug 1999). "ADP-ribosylation factor (ARF)-like 4, 6, ... "Recognition of ADP-ribosylation factor 4-like by HLA-A2-restricted and tumor-reactive cytotoxic T lymphocytes from patients ...
ADP-ribosylation factor-like) family of proteins, which are structurally related to ADP-ribosylation factors (ARFs). ARFs, ... ADP-ribosylation factor-like protein 1 is a protein that in humans is encoded by the ARL1 gene. The protein encoded by this ... Van Valkenburgh H, Shern JF, Sharer JD, Zhu X, Kahn RA (June 2001). "ADP-ribosylation factors (ARFs) and ARF-like 1 (ARL1) have ... Van Valkenburgh H, Shern JF, Sharer JD, Zhu X, Kahn RA (2001). "ADP-ribosylation factors (ARFs) and ARF-like 1 (ARL1) have both ...
ADP ribosylation factor like GTPase 13A is a protein that in humans is encoded by the ARL13A gene. GRCh38: Ensembl release 89: ... ADP ribosylation factor like GTPase 13A". Retrieved 2017-02-09. ...
"Entrez Gene: ARFIP2 ADP-ribosylation factor interacting protein 2 (arfaptin 2)". D'Souza-Schorey, C; Boshans R L; McDonough M; ... Shin, O H; Exton J H (August 2001). "Differential binding of arfaptin 2/POR1 to ADP-ribosylation factors and Rac1". Biochem. ... Kanoh H, Williger BT, Exton JH (April 1997). "Arfaptin 1, a putative cytosolic target protein of ADP-ribosylation factor, is ... Shin OH, Exton JH (2001). "Differential binding of arfaptin 2/POR1 to ADP-ribosylation factors and Rac1". Biochem. Biophys. Res ...
It is a guanine nucleotide exchange factor that promotes binding of GTP to ADP ribosylation factor protein ARF6 and to a lesser ... Hiroi T, Someya A, Thompson W, Moss J, Vaughan M (2006). "GEP100/BRAG2: Activator of ADP-ribosylation factor 6 for regulation ... IQ motif and SEC7 domain-containing protein 1 also known as ARF-GEP100 (ADP-Ribosylation Factor - Guanine nucleotide-Exchange ... a guanine nucleotide-exchange protein for ADP-ribosylation factor 6". Proc. Natl. Acad. Sci. U.S.A. 98 (5): 2413-8. doi:10.1073 ...
"Entrez Gene: ARFGEF1 ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited)". Padilla PI, Chang MJ ... ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. The protein encoded by this gene ... "Isolation of a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP ribosylation factor (ARF) 1 and ARF3 that ... "Purification and cloning of a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP-ribosylation factors". The ...
ADP-ribosylation factor 5 is a protein that in humans is encoded by the ARF5 gene. ADP-ribosylation factor 5 (ARF5) is a member ... "Entrez Gene: ARF5 ADP-ribosylation factor 5". Kanoh, H; Williger B T; Exton J H (February 1997). "Arfaptin 1, a putative ... Shin OH, Couvillon AD, Exton JH (2001). "Arfophilin is a common target of both class II and class III ADP-ribosylation factors ... Shin, O H; Exton J H (August 2001). "Differential binding of arfaptin 2/POR1 to ADP-ribosylation factors and Rac1". Biochem. ...
ARL6IP1: encoding protein ADP-ribosylation factor-like protein 6-interacting protein 1 ... PMFBP1: encoding protein Polyamine-modulated factor 1-binding protein 1. *POLR3K: encoding enzyme DNA-directed RNA polymerase ...
Synonyms: ADP-ribosylation factor-like protein 6-interacting protein 6, AIP-6, ARL-6-interacting protein 6, Aip-6, MGC33864 ...
ADP-ribosylation factor-like 5C. Synonyms: Arl12. Gene nomenclature, locus information, and GO, OMIM, and PMID associations are ... Vega: OTTMUSG2915 (Arl5c, ADP-ribosylation factor-like 5C)*CCDS: 25334, 25334.1*Gene Ontology: Arl5c *Mouse Phenome DB: Arl5c * ...
Myc-DDK-tagged ORF clone of Homo sapiens ADP-ribosylation factor-like 5C (ARL5C) as transfection-ready DNA - 10 µg - OriGene - ... home , products , origene , myc-ddk-tagged orf clone of homo sapiens adp-ribosylation factor-like 5c (arl5c) as transfection- ... Myc-DDK-tagged ORF clone of Homo sapiens ADP-ribosylation factor-like 5C (ARL5C) as transfection-ready DNA - RC227851 - 10 µg ... TrueORF Myc-DDK-tagged ORF clone of Homo sapiens ADP-ribosylation factor-like 5C (ARL5C) as transfection-ready DNA ...
Mouse ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited) (Arfgef1), (10ug), 10 µg. ... Home » cDNA » Mouse cDNA » Arfgef1 (untagged) - Mouse ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A- ... Properties for Arfgef1 (untagged) - Mouse ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited) ( ... MC224976 Arfgef1 (untagged) - Mouse ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited) ( ...
Here, we characterized IQ-ArfGEF/BRAG1, a guanine nucleotide exchange factor (GEF) for Arf6, in the mouse brain. In vivo Arf ... ADP ribosylation factor 6 (Arf6) is a small GTPase that regulates dendritic differentiation possibly through the organization ... ADP ribosylation factor 6 (Arf6) is a small GTPase that regulates dendritic differentiation possibly through the organization ... Here, we characterized IQ-ArfGEF/BRAG1, a guanine nucleotide exchange factor (GEF) for Arf6, in the mouse brain. In vivo Arf ...
Among the small GTP-binding proteins, ARF1 (ADP-ribosylation factor 1) and SAR1 (Secretion-Associated RAS super family 1) are ... Coatomer and dimeric ADP ribosylation factor 1 promote distinct steps in membrane scission. J. Cell Biol 2011, 194, 765-777. [ ... Overexpression of ADP-ribosylation factor 1 in human gastric carcinoma and its clinicopathological. Cancer Sci 2012, 103, 1136- ... 2. ARF1 (ADP-Ribosylation Factor 1) and SAR1 (Secretion-Associated RAS Super Family 1) GTPases. Protein coats are classified ...
ADP-ribosylation factor 1 (ARF1, P84077), PIP2, RhoA, PKC evoked phosphorylation, RalA [1-2] ... Activation of the purified enzymes by phosphatidylinositol 4,5-bisphosphate, ADP-ribosylation factor, and Rho family monomeric ...
ADP ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily. ARF family ... The small ADP ribosylation factor (Arf) GTP-binding proteins are major regulators of vesicle biogenesis in intracellular ... Kahn RA, Kern FG, Clark J, Gelmann EP, Rulka C (1991). "Human ADP-ribosylation factors. A functionally conserved family of GTP- ... Amor JC, Harrison DH, Kahn RA, Ringe D (1994). "Structure of the human ADP-ribosylation factor 1 complexed with GDP". Nature. ...
ARL1 ADP ribosylation factor like GTPase 1 [Homo sapiens] ARL1 ADP ribosylation factor like GTPase 1 [Homo sapiens]. Gene ID: ... ADP-ribosylation factor-like protein 1. Names. ADP-ribosylation factor-like 1. ... ADP-ribosylation factor-like) family of proteins, which are structurally related to ADP-ribosylation factors (ARFs). ARFs, ... ADP ribosylation factor like GTPase 1provided by HGNC. Primary source. HGNC:HGNC:692 See related. Ensembl:ENSG00000120805 MIM: ...
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
ADP-ribosylation factor 4. Names. ADP-ribosylation factor 2. NP_001651.1. *EC 3.6.5.1 ... ARF4 ADP ribosylation factor 4 [Homo sapiens] ARF4 ADP ribosylation factor 4 [Homo sapiens]. Gene ID:378 ... ADP ribosylation factor 4provided by HGNC. Primary source. HGNC:HGNC:655 See related. Ensembl:ENSG00000168374 MIM:601177 Gene ... found ADP-ribosylation factor (ARF) 4 is one of the putative target genes of Mir-221, and the direct binding relationship was ...
ADP-ribosylation factor 6Add BLAST. 174. Amino acid modifications. Feature key. Position(s). DescriptionActions. Graphical view ... sp,P62332,ARF6_RAT ADP-ribosylation factor 6 OS=Rattus norvegicus GN=Arf6 PE=1 SV=2 ... Activation is generally mediated by guanine exchange factor (GEF), while inactivation through hydrolysis of bound GTP is ...
ADP Ribosylation Factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily. ARF family ... Retrieved from "https://www.wikidoc.org/index.php?title=ADP_ribosylation_factor&oldid=157557" ... GTPase activating proteins (GAPs) force ARF to hydrolyze bound GTP to GDP, and Guanine nucleotide exchange factors force ARF to ...
ADP-ribosylation factor-like protein 3. Details. Name. ADP-ribosylation factor-like protein 3. Kind. protein. Organism. Humans ... ADP-ribosylation factor-like protein 3. P36405. Details. Drug Relations. Drug Relations. DrugBank ID. Name. Drug group. ...
ADP-ribosylation factor guanine nucleotide-exchange factor 2 variantImported. ,p>Information which has been imported from ... tr,Q59FR3,Q59FR3_HUMAN ADP-ribosylation factor guanine nucleotide-exchange factor 2 variant (Fragment) OS=Homo sapiens OX=9606 ... ADP ribosylation factor guanine nucleotide exchange factor 2. PANTR. 1792. ADP ribosylation factor guanine nucleotide exchange ...
Compare ADP ribosylation factor like GTPase 13A ELISA Kits from leading suppliers on Biocompare. View specifications, prices, ... ADP ribosylation factor like GTPase 13A ELISA Kits. The ELISA (enzyme-linked immunosorbent assay) is a well-established ... Your search returned 26 ADP ribosylation factor like GTPase 13A ELISA ELISA Kit across 2 suppliers. ... Background: Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a rational target... read more ...
Compare ADP-ribosylation factor like GTPase 11 ELISA Kits from leading suppliers on Biocompare. View specifications, prices, ... ADP-ribosylation factor like GTPase 11 ELISA Kits. The ELISA (enzyme-linked immunosorbent assay) is a widely used application ... Your search returned 24 ADP-ribosylation factor like GTPase 11 ELISA ELISA Kit across 2 suppliers. ...
ADP-RIBOSYLATION FACTOR, ADP-ribosylation factor 1, ADP-RIBOSYLATION FACTOR 1A, arf1, arf2, arf3, ATARF, ATARF1, ATARFA1A, ... ADP-ribosylation factor 1 , adp-ribosylation factor 1 , ADP-ribosylation factor domain protein 1 ... ADP-Ribosylation Factor 1 (ARF1) Antigen Profile Antigen Summary ADP-ribosylation factor 1 (ARF1) is a member of the human ARF ... Browse our anti-ADP-Ribosylation Factor 1 (ARF1) Antibodies. Full name:. anti-ADP-Ribosylation Factor 1 Antibodies (ARF1). On ...
ADP-ribosylation factor-like 2 (ARL2) has recently been implicated in OS formation through its association with Binder of ARL2 ... ADP ribosylation factor like 2 (Arl2) protein influences microtubule dynamics in breast cancer cells. Exp. Cell Res. 313, 473- ... ADP-ribosylation factor-like 2 (ARL2) has recently been implicated in OS formation through its association with Binder of ARL2 ... Specifically, the ADP-ribosylation factor-like 2 (ARL2) small GTPase was implicated in the process of OS formation in ...
ADP-ribosylation factor 4 , ADP-ribosylation factor 2 , ADP-ribosylation factor family-like protein ... ADP-Ribosylation Factor GTPase Activating Protein 3 ELISA Kits * ADP-Ribosylation Factor Guanine Nucleotide-Exchange Factor 1( ... Browse our ADP-Ribosylation Factor 4 (ARF4) ELISA Kits. Full name:. ADP-Ribosylation Factor 4 ELISA Kits (ARF4). On www. ... ADP-Ribosylation Factor GTPase Activating Protein 1 ELISA Kits * ADP-Ribosylation Factor GTPase Activating Protein 2 ELISA Kits ...
ADP-ribosylation factor 4 , ADP-ribosylation factor 2 , ADP-ribosylation factor family-like protein ... ADP-Ribosylation Factor GTPase Activating Protein 3 ELISA Kits * ADP-Ribosylation Factor Guanine Nucleotide-Exchange Factor 1( ... ADP-Ribosylation Factor 4 (ARF4) ELISA Kits. Bezeichnung:. ADP-Ribosylation Factor 4 ELISA Kits (ARF4). Auf www.antikoerper- ... ADP-Ribosylation Factor GTPase Activating Protein 1 ELISA Kits * ADP-Ribosylation Factor GTPase Activating Protein 2 ELISA Kits ...
Synonyms: ADP-ribosylation factor-like protein 13B, ADP-ribosylation factor-like protein 2-like 1, ARL2-like protein 1, ARL2L1 ...
ADP-ribosylation factor-like 4C LOC367311 similar to ADP-ribosylation factor-like protein 4C (ADP-ribosylation factor-like 7) ... ADP-ribosylation factor-like 4C; ADP-ribosylation factor-like 4C, pseudogene 1; ADP-ribosylation factor-like protein 4C; Arl4c- ... ARL4C (ADP ribosylation factor like GTPase 4C). RGD. RGD. Mus musculus (house mouse):. Arl4c (ADP-ribosylation factor-like 4C) ... ADP-ribosylation factor like GTPase 4C Name changed. 629549. APPROVED. 2015-11-19. Arl4c ADP-ribosylation factor like GTPase 4C ...
ADP-ribosylation factor-like 9 explanation free. What is ADP-ribosylation factor-like 9? Meaning of ADP-ribosylation factor- ... Looking for online definition of ADP-ribosylation factor-like 9 in the Medical Dictionary? ... ADP-ribosylation factor-like 9 , definition of ADP-ribosylation factor-like 9 by Medical dictionary https://medical-dictionary. ... redirected from ADP-ribosylation factor-like 9) ARL9. A gene on chromosome 4q12 that encodes a GTPase which is similar to the ...
ADP-ribosylation factor-like 17 explanation free. What is ADP-ribosylation factor-like 17? Meaning of ADP-ribosylation factor- ... Looking for online definition of ADP-ribosylation factor-like 17 in the Medical Dictionary? ... ADP-ribosylation factor-like 17 , definition of ADP-ribosylation factor-like 17 by Medical dictionary https://medical- ... redirected from ADP-ribosylation factor-like 17) ARL17B. A gene on chromosome 17q21.31 that encodes a GTP-binding protein, ...
  • 1.-Marshansky V - Identification of ADP-ribosylation factor-6 in brush-border membrane and early endosomes of human kidney proximal tubules. (medimabs.com)
  • Chemotactic Factor-Induced Recruitment and Activation of Tec Family Kinases in Human Neutrophils. (jimmunol.org)
  • The activation of the human polymorphonuclear neutrophils by the vast majority of the agents inducing its directed migration to sites of injury or infection (chemotactic factors and chemokines) is mediated by their interaction with G protein-coupled receptors (GPCRs) 3 ( 1 ). (jimmunol.org)
  • Of particular relevance to the subject of the present investigation, stimulation of human neutrophils by chemotactic factors has very recently been observed to be associated with the translocation and activation of the PH domain-containing tyrosine kinases of the Tec family termed Tec, Btk and Bmx ( 16 ). (jimmunol.org)
  • PA5-21607 targets ADP-ribosylation factor 3 in WB applications and shows reactivity with Human samples. (fishersci.com)
  • It has previously been shown that human granulocyte-macrophage colony-stimulating factor (GM-CSF) can be fused to a truncated diphtheria toxin (DT) to produce a recombinant fusion toxin that kills GM-CSF receptor-bearing cells. (bloodjournal.org)
  • We chose to use diphtheria toxin as the toxophore and human granulocyte-macrophage colony-stimulating factor (GM-CSF) as the haptophore or ligand. (bloodjournal.org)
  • Cellular levels of phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) are rapidly elevated in response to activation of growth factor receptor tyrosine kinases. (umassmed.edu)
  • Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. (novusbio.com)