MONOMERIC GTP-BINDING PROTEINS that were initially recognized as allosteric activators of the MONO(ADP-RIBOSE) TRANSFERASE of the CHOLERA TOXIN catalytic subunit. They are involved in vesicle trafficking and activation of PHOSPHOLIPASE D. This enzyme was formerly listed as EC 3.6.1.47
ADP-RIBOSYLATION FACTOR 1 is involved in regulating intracellular transport by modulating the interaction of coat proteins with organelle membranes in the early secretory pathway. It is a component of COAT PROTEIN COMPLEX I. This enzyme was formerly listed as EC 3.6.1.47.
A fungal metabolite which is a macrocyclic lactone exhibiting a wide range of antibiotic activity.
Vesicles formed when cell-membrane coated pits (COATED PITS, CELL-MEMBRANE) invaginate and pinch off. The outer surface of these vesicles are covered with a lattice-like network of coat proteins, such as CLATHRIN, coat protein complex proteins, or CAVEOLINS.
A 700-kDa cytosolic protein complex consisting of seven equimolar subunits (alpha, beta, beta', gamma, delta, epsilon and zeta). COATOMER PROTEIN and ADP-RIBOSYLATION FACTOR 1 are principle components of COAT PROTEIN COMPLEX I and are involved in vesicle transport between the ENDOPLASMIC RETICULUM and the GOLGI APPARATUS.
Protein factors that promote the exchange of GTP for GDP bound to GTP-BINDING PROTEINS.
Regulatory proteins that act as molecular switches. They control a wide range of biological processes including: receptor signaling, intracellular signal transduction pathways, and protein synthesis. Their activity is regulated by factors that control their ability to bind to and hydrolyze GTP to GDP. EC 3.6.1.-.
A protein complex comprised of COATOMER PROTEIN and ADP RIBOSYLATION FACTOR 1. It is involved in transport of vesicles between the ENDOPLASMIC RETICULUM and the GOLGI APPARATUS.
A stack of flattened vesicles that functions in posttranslational processing and sorting of proteins, receiving them from the rough ENDOPLASMIC RETICULUM and directing them to secretory vesicles, LYSOSOMES, or the CELL MEMBRANE. The movement of proteins takes place by transfer vesicles that bud off from the rough endoplasmic reticulum or Golgi apparatus and fuse with the Golgi, lysosomes or cell membrane. (From Glick, Glossary of Biochemistry and Molecular Biology, 1990)
Proteins that activate the GTPase of specific GTP-BINDING PROTEINS.
An ester formed between the aldehydic carbon of RIBOSE and the terminal phosphate of ADENOSINE DIPHOSPHATE. It is produced by the hydrolysis of nicotinamide-adenine dinucleotide (NAD) by a variety of enzymes, some of which transfer an ADP-ribosyl group to target proteins.
An enzyme found mostly in plant tissue. It hydrolyzes glycerophosphatidates with the formation of a phosphatidic acid and a nitrogenous base such as choline. This enzyme also catalyzes transphosphatidylation reactions. EC 3.1.4.4.
Guanosine 5'-(trihydrogen diphosphate), monoanhydride with phosphorothioic acid. A stable GTP analog which enjoys a variety of physiological actions such as stimulation of guanine nucleotide-binding proteins, phosphoinositide hydrolysis, cyclic AMP accumulation, and activation of specific proto-oncogenes.
Guanosine 5'-(tetrahydrogen triphosphate). A guanine nucleotide containing three phosphate groups esterified to the sugar moiety.
Esters formed between the aldehydic carbon of sugars and the terminal phosphate of adenosine diphosphate.
Cellular uptake of extracellular materials within membrane-limited vacuoles or microvesicles. ENDOSOMES play a central role in endocytosis.
The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
A pentose active in biological systems usually in its D-form.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
Enzymes that transfer the ADP-RIBOSE group of NAD or NADP to proteins or other small molecules. Transfer of ADP-ribose to water (i.e., hydrolysis) is catalyzed by the NADASES. The mono(ADP-ribose)transferases transfer a single ADP-ribose. POLY(ADP-RIBOSE) POLYMERASES transfer multiple units of ADP-ribose to protein targets, building POLY ADENOSINE DIPHOSPHATE RIBOSE in linear or branched chains.
Transport proteins that carry specific substances in the blood or across cell membranes.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
An ENTEROTOXIN from VIBRIO CHOLERAE. It consists of two major protomers, the heavy (H) or A subunit and the B protomer which consists of 5 light (L) or B subunits. The catalytic A subunit is proteolytically cleaved into fragments A1 and A2. The A1 fragment is a MONO(ADP-RIBOSE) TRANSFERASE. The B protomer binds cholera toxin to intestinal epithelial cells, and facilitates the uptake of the A1 fragment. The A1 catalyzed transfer of ADP-RIBOSE to the alpha subunits of heterotrimeric G PROTEINS activates the production of CYCLIC AMP. Increased levels of cyclic AMP are thought to modulate release of fluid and electrolytes from intestinal crypt cells.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
One of the virulence factors produced by BORDETELLA PERTUSSIS. It is a multimeric protein composed of five subunits S1 - S5. S1 contains mono ADPribose transferase activity.
A set of BACTERIAL ADHESINS and TOXINS, BIOLOGICAL produced by BORDETELLA organisms that determine the pathogenesis of BORDETELLA INFECTIONS, such as WHOOPING COUGH. They include filamentous hemagglutinin; FIMBRIAE PROTEINS; pertactin; PERTUSSIS TOXIN; ADENYLATE CYCLASE TOXIN; dermonecrotic toxin; tracheal cytotoxin; Bordetella LIPOPOLYSACCHARIDES; and tracheal colonization factor.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Adenosine 5'-(trihydrogen diphosphate). An adenine nucleotide containing two phosphate groups esterified to the sugar moiety at the 5'-position.
Enzymes that catalyze the transfer of multiple ADP-RIBOSE groups from nicotinamide-adenine dinucleotide (NAD) onto protein targets, thus building up a linear or branched homopolymer of repeating ADP-ribose units i.e., POLY ADENOSINE DIPHOSPHATE RIBOSE.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Toxic proteins produced from the species CLOSTRIDIUM BOTULINUM. The toxins are synthesized as a single peptide chain which is processed into a mature protein consisting of a heavy chain and light chain joined via a disulfide bond. The botulinum toxin light chain is a zinc-dependent protease which is released from the heavy chain upon ENDOCYTOSIS into PRESYNAPTIC NERVE ENDINGS. Once inside the cell the botulinum toxin light chain cleaves specific SNARE proteins which are essential for secretion of ACETYLCHOLINE by SYNAPTIC VESICLES. This inhibition of acetylcholine release results in muscular PARALYSIS.
A coenzyme composed of ribosylnicotinamide 5'-diphosphate coupled to adenosine 5'-phosphate by pyrophosphate linkage. It is found widely in nature and is involved in numerous enzymatic reactions in which it serves as an electron carrier by being alternately oxidized (NAD+) and reduced (NADH). (Dorland, 27th ed)
An enzyme of the lyase class that catalyzes the formation of CYCLIC AMP and pyrophosphate from ATP. EC 4.6.1.1.
One of the virulence factors produced by virulent BORDETELLA organisms. It is a bifunctional protein with both ADENYLYL CYCLASES and hemolysin components.
A polynucleotide formed from the ADP-RIBOSE moiety of nicotinamide-adenine dinucleotide (NAD) by POLY(ADP-RIBOSE) POLYMERASES.
A phosphoinositide present in all eukaryotic cells, particularly in the plasma membrane. It is the major substrate for receptor-stimulated phosphoinositidase C, with the consequent formation of inositol 1,4,5-triphosphate and diacylglycerol, and probably also for receptor-stimulated inositol phospholipid 3-kinase. (Kendrew, The Encyclopedia of Molecular Biology, 1994)
Toxic substances formed in or elaborated by bacteria; they are usually proteins with high molecular weight and antigenicity; some are used as antibiotics and some to skin test for the presence of or susceptibility to certain diseases.
The rate dynamics in chemical or physical systems.
A class of proteins involved in the transport of molecules via TRANSPORT VESICLES. They perform functions such as binding to the cell membrane, capturing cargo molecules and promoting the assembly of CLATHRIN. The majority of adaptor proteins exist as multi-subunit complexes, however monomeric varieties have also been found.
A RHO GTP-BINDING PROTEIN involved in regulating signal transduction pathways that control assembly of focal adhesions and actin stress fibers. This enzyme was formerly listed as EC 3.6.1.47.
Proteins prepared by recombinant DNA technology.
One of the early purine analogs showing antineoplastic activity. It functions as an antimetabolite and is easily incorporated into ribonucleic acids.
Thin structures that encapsulate subcellular structures or ORGANELLES in EUKARYOTIC CELLS. They include a variety of membranes associated with the CELL NUCLEUS; the MITOCHONDRIA; the GOLGI APPARATUS; the ENDOPLASMIC RETICULUM; LYSOSOMES; PLASTIDS; and VACUOLES.
Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
Established cell cultures that have the potential to propagate indefinitely.
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
A group of telomere associated proteins that interact with TRF1 PROTEIN, contain ANKYRIN REPEATS and have poly(ADP-ribose) polymerase activity.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). The species is a favorite for cytogenetic studies because of its small chromosome number. The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells.
A class of nucleotide translocases found abundantly in mitochondria that function as integral components of the inner mitochondrial membrane. They facilitate the exchange of ADP and ATP between the cytosol and the mitochondria, thereby linking the subcellular compartments of ATP production to those of ATP utilization.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.

Identification of a new Pyk2 target protein with Arf-GAP activity. (1/1111)

Protein tyrosine kinase Pyk2 is activated by a variety of G-protein-coupled receptors and by extracellular signals that elevate intracellular Ca2+ concentration. We have identified a new Pyk2 binding protein designated Pap. Pap is a multidomain protein composed of an N-terminal alpha-helical region with a coiled-coil motif, followed by a pleckstrin homology domain, an Arf-GAP domain, an ankyrin homology region, a proline-rich region, and a C-terminal SH3 domain. We demonstrate that Pap forms a stable complex with Pyk2 and that activation of Pyk2 leads to tyrosine phosphorylation of Pap in living cells. Immunofluorescence experiments demonstrate that Pap is localized in the Golgi apparatus and at the plasma membrane, where it is colocalized with Pyk2. In addition, in vitro recombinant Pap exhibits strong GTPase-activating protein (GAP) activity towards the small GTPases Arf1 and Arf5 and weak activity towards Arf6. Addition of recombinant Pap protein to Golgi preparations prevented Arf-dependent generation of post-Golgi vesicles in vitro. Moreover, overexpression of Pap in cultured cells reduced the constitutive secretion of a marker protein. We propose that Pap functions as a GAP for Arf and that Pyk2 may be involved in regulation of vesicular transport through its interaction with Pap.  (+info)

ARF6 requirement for Rac ruffling suggests a role for membrane trafficking in cortical actin rearrangements. (2/1111)

The ARF6 GTPase regulates a novel endosomal-plasma membrane recycling pathway and influences cortical actin remodeling. Here we examined the relationship between ARF6 and Rac1, a Rho family GTPase, implicated in cortical actin rearrangements. Endogenous Rac1 colocalized with ARF6 at the plasma membrane and on the ARF6 recycling endosome in untransfected HeLa and primary human fibroblast cells. In transfected HeLa cells Rac1 and ARF6 also colocalized. Cells expressing wild-type ARF6 or Rac1 formed actin-containing surface protrusions and membrane ruffles, respectively, upon treatment with the G protein activator aluminum fluoride. Aluminum fluoride-treatment of cells transfected with equivalent amounts of plasmid resulted in enhanced membrane ruffling, with protrusions appearing as Rac expression was lowered. Co-expression of the dominant negative, GTP binding-defective ARF6 T27N mutant inhibited the aluminum fluoride-induced ruffling observed in cells expressing Rac1, and the constitutive ruffling observed in cells expressing the activated Rac1 Q61L mutant. In contrast, co-expression of the GTP-binding-defective, T17N mutant of either Rac1 or Cdc42 with ARF6 did not inhibit the aluminum fluoride-induced surface protrusions, nor did inactivation of Rho with C3-transferase. These observations suggest that ARF6, a non-Rho family GTPase, can, by itself, alter cortical actin and can influence the ability of Rac1 to form lamellipodia, in part, by regulating its trafficking to the plasma membrane.  (+info)

Coupling of coat assembly and vesicle budding to packaging of putative cargo receptors. (3/1111)

COPI-coated vesicle budding from lipid bilayers whose composition resembles mammalian Golgi membranes requires coatomer, ARF, GTP, and cytoplasmic tails of putative cargo receptors (p24 family proteins) or membrane cargo proteins (containing the KKXX retrieval signal) emanating from the bilayer surface. Liposome-derived COPI-coated vesicles are similar to their native counterparts with respect to diameter, buoyant density, morphology, and the requirement for an elevated temperature for budding. These results suggest that a bivalent interaction of coatomer with membrane-bound ARF[GTP] and with the cytoplasmic tails of cargo or putative cargo receptors is the molecular basis of COPI coat assembly and provide a simple mechanism to couple uptake of cargo to transport vesicle formation.  (+info)

GCS1, an Arf guanosine triphosphatase-activating protein in Saccharomyces cerevisiae, is required for normal actin cytoskeletal organization in vivo and stimulates actin polymerization in vitro. (4/1111)

Recent cloning of a rat brain phosphatidylinositol 3,4, 5-trisphosphate binding protein, centaurin alpha, identified a novel gene family based on homology to an amino-terminal zinc-binding domain. In Saccharomyces cerevisiae, the protein with the highest homology to centaurin alpha is Gcs1p, the product of the GCS1 gene. GCS1 was originally identified as a gene conditionally required for the reentry of cells into the cell cycle after stationary phase growth. Gcs1p was previously characterized as a guanosine triphosphatase-activating protein for the small guanosine triphosphatase Arf1, and gcs1 mutants displayed vesicle-trafficking defects. Here, we have shown that similar to centaurin alpha, recombinant Gcs1p bound phosphoinositide-based affinity resins with high affinity and specificity. A novel GCS1 disruption strain (gcs1Delta) exhibited morphological defects, as well as mislocalization of cortical actin patches. gcs1Delta was hypersensitive to the actin monomer-sequestering drug, latrunculin-B. Synthetic lethality was observed between null alleles of GCS1 and SLA2, the gene encoding a protein involved in stabilization of the actin cytoskeleton. In addition, synthetic growth defects were observed between null alleles of GCS1 and SAC6, the gene encoding the yeast fimbrin homologue. Recombinant Gcs1p bound to actin filaments, stimulated actin polymerization, and inhibited actin depolymerization in vitro. These data provide in vivo and in vitro evidence that Gcs1p interacts directly with the actin cytoskeleton in S. cerevisiae.  (+info)

EFA6, a sec7 domain-containing exchange factor for ARF6, coordinates membrane recycling and actin cytoskeleton organization. (5/1111)

We have identified a human cDNA encoding a novel protein, exchange factor for ARF6 (EFA6), which contains Sec7 and pleckstrin homology domains. EFA6 promotes efficient guanine nucleotide exchange on ARF6 and is distinct from the ARNO family of ARF1 exchange factors. The protein localizes to a dense matrix on the cytoplasmic face of plasma membrane invaginations, induced on its expression. We show that EFA6 regulates endosomal membrane recycling and promotes the redistribution of transferrin receptors to the cell surface. Furthermore, expression of EFA6 induces actin-based membrane ruffles that are inhibited by co-expression of dominant-inhibitory mutant forms of ARF6 or Rac1. Our results demonstrate that by catalyzing nucleotide exchange on ARF6 at the plasma membrane and by regulating Rac1 activation, EFA6 coordinates endocytosis with cytoskeletal rearrangements.  (+info)

Structural basis for the inhibitory effect of brefeldin A on guanine nucleotide-exchange proteins for ADP-ribosylation factors. (6/1111)

Protein secretion through the endoplasmic reticulum and Golgi vesicular trafficking system is initiated by the binding of ADP-ribosylation factors (ARFs) to donor membranes, leading to recruitment of coatomer, bud formation, and eventual vesicle release. ARFs are approximately 20-kDa GTPases that are active with bound GTP and inactive with GDP bound. Conversion of ARF-GDP to ARF-GTP is regulated by guanine nucleotide-exchange proteins. All known ARF guanine nucleotide-exchange proteins contain a Sec7 domain of approximately 200 amino acids that includes the active site and fall into two classes that differ in molecular size and susceptibility to inhibition by the fungal metabolite brefeldin A (BFA). To determine the structural basis of BFA sensitivity, chimeric molecules were constructed by using sequences from the Sec7 domains of BFA-sensitive yeast Sec7 protein (ySec7d) and the insensitive human cytohesin-1 (C-1Sec7). Based on BFA inhibition of the activities of these molecules with recombinant yeast ARF2 as substrate, the Asp965-Met975 sequence in ySec7d was shown to be responsible for BFA sensitivity. A C-1Sec7 mutant in which Ser199, Asn204, and Pro209 were replaced with the corresponding ySec7d amino acids, Asp965, Gln970, and Met975, exhibited BFA sensitivity similar to that of recombinant ySec7d (rySec7d). Single replacement in C-1Sec7 of Ser199 or Pro209 resulted in partial inhibition by BFA, whereas replacement of Gln970 in ySec7d with Asn (as found in C-1Sec7) had no effect. As predicted, the double C-1Sec7 mutant with S199D and P209M was BFA-sensitive, demonstrating that Asp965 and Met975 in ySec7d are major molecular determinants of BFA sensitivity.  (+info)

Characterization of the regulation of phospholipase D activity in the detergent-insoluble fraction of HL60 cells by protein kinase C and small G-proteins. (7/1111)

Phospholipase D (PLD) activity has been shown to be GTP-dependent both in vivo and in vitro. One protein that confers GTP sensitivity to PLD activity in vitro is the low-molecular-mass G-protein ADP-ribosylation factor (Arf). However, members of the Rho family and protein kinase C (PKC) have also been reported to activate PLD in various cell systems. We have characterized the stimulation of PLD in HL60 cell membranes by these proteins. The results demonstrate that a considerable proportion of HL60 PLD activity is located in a detergent-insoluble fraction of the cell membrane that is unlikely to be a caveolae-like domain, but is probably cytoskeletal. This PLD activity required the presence of Arf1, a Rho-family member and PKC for efficient catalysis of the lipid substrate, suggesting that the activity represents PLD1. We show that recombinant human PLD1b is regulated in a similar manner to HL60-membrane PLD, and that PKCalpha and PKCdelta are equally effective PLD activators. Therefore maximum PLD activity requires Arf, a Rho-family member and PKC, emphasizing the high degree of regulation of this enzyme.  (+info)

The ADP-ribosylation factor (ARF)-related GTPase ARF-related protein binds to the ARF-specific guanine nucleotide exchange factor cytohesin and inhibits the ARF-dependent activation of phospholipase D. (8/1111)

ADP-ribosylation factor-related protein (ARP) is a membrane-associated GTPase with remote similarity to the family of ADP-ribosylation factors (ARF). In a yeast two-hybrid screen designed to identify proteins interacting with ARP, we isolated a partial cDNA of the ARF-specific guanine nucleotide exchange factor mSec7-1/cytohesin encoding its N terminus and most of the Sec7 domain (codons 1-200). ARP and ARP-Q79L (GTPase-negative ARP) exhibited a higher affinity to mSec7-1-(1-200) than ARP-T31N (nucleotide exchange-defective ARP) in the two-hybrid assay. Similarly, full-length [35S]mSec7-1/cytohesin was specifically adsorbed to glutathione-Sepharose loaded with glutathione S-transferase (GST)-ARP-Q79L, GST-ARP, or GST-ARP-T31N, the latter exhibiting the lowest binding affinity. Overexpression of ARP-Q79L, but not of ARP-T31N, in COS-7 cells reduced the fluorescence from co-expressed green fluorescent protein fused with mSec7-1/cytohesin or mSec7-2/ARNO in plasma membranes as detected by deconvolution microscopy. Recombinant ARP and ARP-Q79L, but not ARP-T31N, inhibited the phospholipase D (PLD) activity stimulated by mSec7-2/ARNO and ARF in a system of isolated membranes. Furthermore, transfection of HEK-293 cells with ARP or ARP-Q79L, but not ARP-T31N, inhibited the muscarinic acetylcholine receptor-3 induced PLD stimulation and translocation of ARF from cytosol to membranes. These data suggest that the GTP-bound form of ARP specifically binds mSec7-1/cytohesin, and that ARP may be involved in a pathway inhibiting the ARF-controlled activity of PLD.  (+info)

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Buy ARFGAP1 recombinant protein, ADP-ribosylation factor GTPase-activating protein 1 (ARFGAP1) Recombinant Protein-NP_001268411.1 (MBS1301288) product datasheet at MyBioSource, Recombinant Proteins
ID: http://www.ncbi.nlm.nih.gov/gene/390790 Type: http://bio2vec.net/ontology/gene Label: ARL5C Synonyms: ARL5C, ARL12, ADP ribosylation factor like GTPase 5C, putative ADP-ribosylation factor-like protein 5C, ADP-ribosylation factor-like 5C, ADP-ribosylation factor-like protein 12 Alternative IDs: als API: GO SPARQL: GO ...
Myc-DDK-tagged ORF clone of Homo sapiens ADP-ribosylation factor-like 5C (ARL5C) as transfection-ready DNA - 10 µg - OriGene - cdna clones
Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). Required for normal cytokinesis and cilia signaling. Requires assistance from GTPase-activating proteins (GAPs) like RP2 and PDE6D, in order to cycle between inactive GDP-bound and active GTP-bound forms. Required for targeting proteins such as NPHP3 to the ciliary membrane by releasing myristoylated NPHP3 from UNC119B cargo adapter into the cilium. Does not act as an allosteric activator of the cholera toxin catalytic subunit ...
Guanine nucleotide exchange factor for Arf GTPases, stimulating the nucleotide exchange from the GDP-bound to the GTP-bound form. Catalyzes both the GDP release by and the GTP binding to ARF2. Has no exhange activity on Rab GTPases. Involved in vesicular transport.
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The KOMP Repository is located at the University of California Davis and Childrens Hospital Oakland Research Institute. Question? Comments? For Mice, Cells, and germplasm please contact us at [email protected], US 1-888-KOMP-MICE or International +1-530-752-KOMP, or for vectors [email protected] or +1-510-450-7917 ...
TY - JOUR. T1 - ADP ribosylation factor 6 regulates neuronal migration in the developing cerebral cortex through FIP3/arfophilin-1-dependent endosomal trafficking of N-cadherin. AU - Hara, Yoshinobu. AU - Fukaya, Masahiro. AU - Hayashi, Kanehiro. AU - Kawauchi, Takeshi. AU - Nakajima, Kazunori. AU - Sakagami, Hiroyuki. PY - 2016. Y1 - 2016. N2 - During neural development, endosomal trafficking controls cell shape and motility through the polarized transport of membrane proteins related to cellcell and cellextracellular matrix interactions. ADP ribosylation factor 6 (Arf6) is a critical small GTPase that regulates membrane trafficking between the plasma membrane and endosomes. We herein demonstrated that the knockdown of endogenous Arf6 in mouse cerebral cortices led to impaired neuronal migration in the intermediate zone and cytoplasmic retention of N-cadherin and syntaxin12 in migrating neurons. Rescue experiments with separation-of-function Arf6 mutants identified Rab11 familyinteracting ...
Ver más] The small GTP-binding protein ADP-ribosylation factor 1 (ARF1) is an essential component of the molecular machinery that catalyzes the formation of membranebound transport intermediates. By using an in vitro assay that reproduces recruitment of cytosolic proteins onto purified, high salt-washed Golgi membranes, we have analyzed the role of cAMP-dependent protein kinase A (PKA) on ARF1 incorporation. Addition to this assay of either pure catalytic subunits of PKA (C-PKA) or cAMP increased ARF1 binding. By contrast, ARF1 association was inhibited following C-PKA inactivation with either PKA inhibitory peptide or RIIa as well as after cytosol depletion of C-PKA. C-PKA also stimulated recruitment and activation of a recombinant form of human ARF1 in the absence of additional cytosolic components. The binding step could be dissociated from the activation reaction and found to be independent of guanine nucleotides and saturable. This step was stimulated by C-PKA in an ATP-dependent manner. ...
ADP-ribosylation factors (ARFs) are approximately 20-kDa guanine nucleotide-binding proteins recognized as critical components in intracellular vesicular transport and phospholipase D activation. Both guanine nucleotide-exchange proteins and GTPase-activating proteins (GAPs) for ARFs have been cloned recently. A zinc finger motif near the amino terminus of the ARF1 GAP was required for stimulation of GTP hydrolysis. ARD1 is an ARF family member that differs from other ARFs by the presence of a 46-kDa amino-terminal extension. We had reported that the ARF domain of ARD1 binds specifically GDP and GTP and that the amino-terminal extension acts as a GAP for the ARF domain of ARD1 but not for ARF proteins. The GAP domain of ARD1, synthesized in Escherichia coli, stimulated hydrolysis of GTP bound to the ARF domain of ARD1. Using ARD1 truncations, it appears that amino acids 101-190 are critical for GAP activity, whereas residues 190-333 are involved in physical interaction between the two domains of ...
ADP-ribosylation factors (ARFs) are approximately 20-kDa guanine nucleotide-binding proteins recognized as critical components in intracellular vesicular transport and phospholipase D activation. Both guanine nucleotide-exchange proteins and GTPase-activating proteins (GAPs) for ARFs have been cloned recently. A zinc finger motif near the amino terminus of the ARF1 GAP was required for stimulation of GTP hydrolysis. ARD1 is an ARF family member that differs from other ARFs by the presence of a 46-kDa amino-terminal extension. We had reported that the ARF domain of ARD1 binds specifically GDP and GTP and that the amino-terminal extension acts as a GAP for the ARF domain of ARD1 but not for ARF proteins. The GAP domain of ARD1, synthesized in Escherichia coli, stimulated hydrolysis of GTP bound to the ARF domain of ARD1. Using ARD1 truncations, it appears that amino acids 101-190 are critical for GAP activity, whereas residues 190-333 are involved in physical interaction between the two domains of ...
ADP-ribosylation factor-like protein 4A (ARL4A) is a developmentally regulated member of the ARF/ARL GTPase family. The primary structure of ARL4A is very similar to that of other ARF/ARL molecules, but its function remains unclear. The trans-Golgi network golgin GCC185 is required for maintenance of Golgi structure and distinct endosome-to-Golgi transport. We show here that GCC185 acts as a new effector for ARL4 to modulate Golgi organization. ARL4A directly interacts with GCC185 in a GTP-dependent manner. Sub-coiled-coil regions of the CC2 domain of GCC185 are required for the interaction between GCC185 and ARL4A. Depletion of ARL4A reproduces the GCC185-depleted phenotype, causing fragmentation of the Golgi compartment and defects in endosome-to-Golgi transport. GCC185 and ARL4A localize to the Golgi independently of each other. Deletion of the ARL4A-interacting region of GCC185 results in inability to maintain Golgi structure. Depletion of ARL4A impairs the interaction between GCC185 and ...
TY - JOUR. T1 - Active Arf6 recruits ARNO/cytohesin GEFs to the PM by binding their PH domains. AU - Cohen, Lee Ann. AU - Honda, Akira. AU - Várnai, P.. AU - Brown, Fraser D.. AU - Balla, Tamas. AU - Donaldson, Julie G.. PY - 2007/6. Y1 - 2007/6. N2 - ARNO is a soluble guanine nucleotide exchange factor (GEF) for the Arf family of GTPases. Although in biochemical assays ARNO prefers Arf1 over Arf6 as a substrate, its localization in cells at the plasma membrane (PM) suggests an interaction with Arf6. In this study, we found that ARNO activated Arf1 in HeLa and COS-7 cells resulting in the recruitment of Arf1 on to dynamic PM ruffles. By contrast, Arf6 was activated less by ARNO than EFA6, a canonical Arf6 GEF. Remarkably, Arf6 in its GTP-bound form recruited ARNO to the PM and the two proteins could be immunoprecipitated. ARNO binding to Arf6 was not mediated through the catalytic Sec7 domain, but via the pleckstrin homology (PH) domain. Active Arf6 also bound the PH domain of Grp1, another ...
ADP-ribosylation factor (ARF) and ARF-like (ARL) proteins are small GTPases that undergo a GDP/GTP nucleotide exchange cycle. ARF proteins are important regulators of cellular trafficking. Knowledge of plant ARF proteins and their regulators is emerging from genetic and cellular studies. The Arabidopsis BIG2 ARF-guanine nucleotide exchange factor (GEF) gene is expressed in several tissues and the encoded protein is a typical Arabidopsis ARF-GEF. We address here the specificity of BIG2 for different Arabidopsis ARF proteins. The in vitro effect of the catalytic Sec7 domain of BIG2 on the guanine nucleotide exchange rate of five ARF and ARL proteins was measured using real time fluorescence spectroscopy. The Sec7 domain catalyzed nucleotide exchange on ARF1, but had essentially no effect on the exchange rate of ARF8, ARF9, ARL1, and ARL8a. In Western blots with an anti-BIG2 polyclonal antibody, a BIG2 fraction was detected in membranes, especially those deriving from the Golgi apparatus. The ...
This gene encodes a member of an ADP-ribosylation factor GTPase-activating protein family involved in membrane trafficking and cytoskeleton dynamics. This gene functions as a direct regulator of the adaptor-related protein complex 3 on endosomes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011 ...
ARF1 - ARF1 (untagged)-Human ADP-ribosylation factor 1 (ARF1), transcript variant 4 available for purchase from OriGene - Your Gene Company.
We have demonstrated previously the potent activation of PLD by the chemokine IL-8 in T lymphocytes (18). We have now extended our findings to include the C-C chemokine RANTES in demonstrating that in the Jurkat T cell line, the activation of this enzyme occurs at subnanomolar concentrations and is dependent on the activation of small GTP-binding protein cofactors. RANTES-induced PLD activation is consistently maximal at 1 nM, a concentration corresponding to the optimal chemotaxis-inducing dose in normal T lymphocytes. Interestingly, PLD activation in T lymphocytes and Jurkat T cells appears to be an important biologic consequence of chemokine action and more readily measurable (at nanomolar concentrations) than readouts of receptor activation such as calcium flux. It was also apparent that RANTES is the only chemokine tested to date (RANTES, MIP-1α, MIP-1β, MCP-1, MCP-3, lymphotactin) that induces as robust a response as seen in this study, although the others listed were capable of low ...
Arl1 (ARF like protein1) is a poorly understood member of ARF family small GTPases. This thesis presents an original characterization of Arl1 and its effectors. Arl1 was localized to the tans Golgi under EM. Over expression of guanine nucleotide mutants of Arl1 dramatically affects the structure and function of Golgi apparatus. Arl1-GTP was found to interact with GRIP domain of Golgins (Golgin-97, Golgin-245, GCC1 and KIAA0336). The interaction was dependent on the conserved amino acids on both switch II region of Arl1 and the GRIP domain. Collectively, the research presented in this thesis reveals Arl1 is a new regulator of Golgi structure and function and one mechanism of Arl1a??s function is that it recruits and regulates its effectors a?? GRIP domain Golgins to Golgi ...
Human gamma-adaptin-related protein 2 ELISA Kit;Human golgi-localized, gamma ear-containing, ARF-binding protein 2 ELISA Kit;Human VHS domain and ear domain of gamma-adaptin ELISA Kit;Human VEAR ELISA Kit;Human KIAA1080 ELISA Kit;Human golgi associated, gamma adaptin ear containing, ARF binding protein 2 ELISA Kit;Human ADP-ribosylation factor-binding protein GGA2 ELISA Kit;Human VHS domain and ear domain-containing protein ELISA Kit ...
Abbreviations: ABC, ATP-binding cassette transporter; ANGPTL3, angiopoietin-like 3; apo, apolipoprotein; ARL7, ADP-ribosylation factor-like 7; CAD, coronary artery disease; CXCR4, CXC receptor 4; EC, endothelial cell; FOXA2, forkhead box A2; GPAM, glycerol-3-phosphate acyltransferase 1; HDL, high-density lipoprotein; HDL-C, HDL-cholesterol; HMGCR, 3-hydroxy-3-methylglutaryl-CoA reductase; IL, interleukin; LDL, low-density lipoprotein; LDLR, LDL receptor; LNA, locked nucleic acid; LOX-1, oxidized LDL receptor 1; LPL, lipoprotein lipase; LPS, lipopolysaccharide; LXR, liver X receptor; Mct1, monocarboxylate transporter 1; MI, myocardial infarction; miRNA, microRNA; Mtpn, myotrophin; NFAT, nuclear factor of activated T-cells; NF-κB, nuclear factor κB; p27Kip1, cyclin-dependent kinase inhibitor 1B; p57Kip2, cyclin-dependent kinase inhibitor 1C; oxLDL, oxidized LDL; PEPCK, phosphoenolpyruvate carboxykinase; PI3K, phosphoinositide 3-kinase; PPAR, peroxisome proliferator-activated receptor; pre-miRNA, ...
The small GTP-binding proteins of the ARF family play a central role in membrane dynamics and protein transport in eukaryotic cells. The GEFs that catalyze GDP/GTP exchange on ARF are of critical importance to ARF function, as they determine when and where ARF proteins will be activated within the cell (reviewed by Jackson and Casanova, 2000). We present here an in vivo characterization of the Gea1p and Gea2p ARF GEFs in the secretory pathway of S. cerevisiae. The Gea1p and Gea2p proteins are members of a subfamily of ARF GEFs with members in plants and animals, as well as in yeast ( Claude et al., 1999; Steinmann et al., 1999). This subfamily is distinct from that of Sec7p and its mammalian orthologues BIG1 and BIG2 ( Mansour et al., 1999; Morinaga et al., 1997; Togawa et al., 1999).. The Gea1p and Gea2p proteins are functionally redundant, but at least one is necessary for viability in yeast. We have generated three temperature-sensitive gea mutants and have examined their phenotypes. The ...
D. Jones, B. Bax, S. Cockcroft; ADP-ribosylation factor GTPases in signal transduction and membrane traffic: independent functions?. Biochem Soc Trans 1 August 1999; 27 (4): 642-647. doi: https://doi.org/10.1042/bst0270642. Download citation file:. ...
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FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the GIT protein family, which interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. GIT proteins traffic between cytoplasmic complexes, focal adhesions, and the cell periphery, and interact with Pak interacting exchange factor beta (PIX) to form large oligomeric complexes that transiently recruit other proteins. GIT proteins regulate cytoskeletal dynamics and participate in receptor internalization and membrane trafficking. This gene has been shown to repress lamellipodial extension and focal adhesion turnover, and is thought to regulate cell motility. This gene undergoes extensive alternative splicing to generate multiple isoforms, but the full-length nature of some of these variants has not been determined. The various isoforms have functional differences, with respect to ARF GAP activity ...
Rafiq et al. demonstrate that the small G protein ARF1 and its activator, cytohesin 2 (ARNO), are required for podosome formation in macrophage-like cells and fibroblasts. Inhibition of ARNO-ARF1 signaling results in increased RhoA activity and disassembly of podosomes in a myosin-IIA-dependent fashion. In fibroblasts that normally do not form podosomes, constitutively active ARF1 induces actin-rich puncta associated with sites of matrix degradation, putative precursors of podosomes. ...
Cell migration is an orchestrated and highly coordinated multi-step process that is central to the development and maintenance of multicellular organisms. Dysregulated migration however, is associated with pathological states such as tumor formation and metastasis; thus a clear understanding of the molecular mechanisms that drive this process is critical to the development of counteracting therapeutics. Cell migration and adhesion-dependent cell spreading share a number of features. For example, both processes rely on the activation of mechanisms for the coordinated spatial and temporal assembly/disassembly of focal adhesions, as well as mechanisms controlling actin rearrangements and directed vesicular trafficking. Actin remodeling and vesicular trafficking events are in turn, implicated functions of a variety of small GTPases of the Ras superfamily, which include the Rho and Arf subfamilies. Thus towards efforts of further characterizing the molecular pathways that drive cell spreading, I pursued aims
Two closely related human Arls, Arl8a and Arl8b, were found to localise to lysosomes in mammalian cells. conventionally, membrane binding of Arf and Arl proteins is mediated by both an N-terminal myristoyl group and an N-terminal amphipathic helix that are inserted into the lipid bilayer upon activation of the GTPase. Arl8 GPTases lack myristolylation sites, and examination of the N-terminus of Arl8b revealed that it contains an acetyl group instead, and this acetylated methionine is necessary for its lysosomal location. Lysosomes of cells overexpressing Arl8b move more frequently, suggesting a role for Arl8a and Arl8b as positive regulators of lysosomal transport. Arl4a, Arl4c and Arl4d are very similar in sequence and were found to act in a pathway upstream of Arf6, Arf6 is a regulator of key processes at the plasma membrane, such as endocytosis, actin dynamics and cell adhesion. One of the major activators of Arf6 is the exchange factor ARNO (Arf nucleotide binding site opener). In order to ...
NewEast Biosciences Arf 6 Activation Assay Kit bases on the configuration-specific anti-Arf 6-GTP monoclonal antibody to measure the active Arf 6-GTP levels, either from cell extracts or from in vitro GTPγS loading Arf 6 activation assays. Briefly, anti-active Arf 6 mouse monoclonal antibody will be incubated with cell lysates containing Arf 6-GTP. The bound active Arf 6 will then be pulled down by protein A/G agarose. The precipitated active Arf 6 will be detected by immunoblot analysis using an anti-Arf 6 rabbit polyclonal antibody, respectively. ...
COPⅠ囊泡:最初研究者利用三磷酸鳥苷(GTP)衍生物GTPγS(一種富含高爾基體膜的細胞質與抗水解的GTP衍生物)共培養時,發現高爾基體池之間存在一種囊泡轉運結構[9](後來在真核細胞中也證實此結構的存在[10])。除了脂質成分外,參與此囊泡形成的成份還有7種外被體蛋白(即外被體α、β、β′、γ、δ、ε、ζ)。這些外被體蛋白相互作用形成的復合物就是COPⅠ囊泡[11][12]。亞單位α、β′、ε在結構上與網格蛋白及COPⅡ囊泡的外層組分具有較高的一致性,形成復合物的內層組分稱為B亞復合物(主要負責與靶蛋白結合),而亞單位β、γ、δ、ζ 與網格蛋白及COPⅡ囊泡的內層組分相似,形成復合物的內層組分稱為F亞復合物,該亞復合物主要負責與靶蛋白結合,並且直接與COPⅠ囊泡形成的招募者ADP核糖基化因子(英语:ADP ribosylation factor)(ADP ribosylation ...
Arf-like protein 1 (Arl1) is a member of the Arf family of regulatory GTPases, within the Ras superfamily of GTPases, and with highly conserved orthologs throughout eukaryotes. Arl1 is essential for early embryonic development in Drosophila and in Caenorhabditis elegans. Arl1 is most similar in primary sequence, cellular location, and function (regulation of membrane traffic) to Arf1-6 and even shares several common binding partners. In addition to its function in membrane traffic at the Golgi/trans-Golgi network, there are reports indicating a possible role for Arl1 in ion homeostasis in yeast. ...
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy ...
Other than a role for Ca2+ store depletion, the molecular mechanisms that regulate antigen-stimulated Ca2+ influx into mast cells are not well-understood. The observation that CT dramatically enhances 45Ca2+ influx into RBL-2H3 cells suggests that this reagent might be a useful tool to study the Ca2+ entry pathway (Narasimhan et al., 1988). That CT amplifies both antigen-evoked ICRAC and 45Ca2+ influx to a similar extent bolsters the idea that CRAC channels are a major pathway for FcεRI-mediated Ca2+ uptake into RBL-2H3 mast cells (Zhang and McCloskey, 1995).. Two hypotheses to explain the effect of CT on 45Ca2+ influx are immediately testable by patch clamping. First, it is possible that CT activates Cl− or K+ channels, and thereby increases the electrical force propelling Ca2+ entry. This indirect mechanism cannot explain the enhancement of Ca2+ influx currents that we observed, because voltage-clamp measurements eliminate any difference in membrane potential between control and CT-treated ...
CONTACT: [email protected] GENBANK UPDATE: 22 July 1997 28 unique yeast ORFs ORF NAME LOCUS PRODUCT/DESCRIPTION -------- ----- ---------------------------------------- YFL039C ACT1 Actin YDL192W ARF1 ADP-ribosylation factor YDL137W ARF2 ADP-ribosylation factor 2 YJR121W ATP2 F(1)F(0)-ATPase complex beta subunit, mitochondrial YER177W BMH1 Homolog of mammalian 14-3-3 proteins YLR229C CDC42 member of the Rho subfamily of Ras-like proteins YER133W GLC7 protein phosphatase type I YLR293C GSP1 GTP-binding protein YOR185C GSP2 GTP-binding protein YBR009C HHF1 Histone H4 (HHF1 and HHF2 code for identical proteins) YNL030W HHF2 Histone H4 (HHF1 and HHF2 code for identical proteins) YBR010W HHT1 Histone H3 (HHT1 and HHT2 code for identical proteins) YNL031C HHT2 Histone H3 (HHT1 and HHT2 code for identical proteins) YDR224C HTB1 Histone H2B (HTB1 and HTB2 code for nearly identical proteins) YBL002W HTB2 Histone H2B (HTB1 and HTB2 code for nearly identical proteins) YBL087C RPL17A Ribosomal protein ...
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Description : 29.4 protein.postranslational modification GTPase required for Golgi targeting of GRIP domain proteins. AtARL1 binds directly to the GRIP domain of AtGRIP in a GTP-dependent manner ADP-ribosylation factor 3 (ARF3). ...
Complete information for ARF6 gene (Protein Coding), ADP Ribosylation Factor 6, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Complete information for ARL6IP6 gene (Protein Coding), ADP Ribosylation Factor Like GTPase 6 Interacting Protein 6, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
ARFGAP3 antibody, C-term (ADP-ribosylation factor GTPase activating protein 3) for WB. Anti-ARFGAP3 pAb (GTX89988) is tested in Mouse samples. 100% Ab-Assurance.
Homo sapiens golgi associated, gamma adaptin ear containing, ARF binding protein 1 (GGA1), transcript variant 2, mRNA. (H00026088-R01) - Products - Abnova
I want to BRAG big time about Becky and Sheila for great Tourism Currents track at Blogworld LA. Their positive energy comes through in every tweet and photos people are sharing.. I also want to brag again on my girl Jeanne Robertson. She was in 5 cities in 5 days. She is remarkable. btw, at 68 she is busier and more popular than ever. She credits modern tools like Facebook and YouTube for her new career. She has been a professional speaker for 48 years.. Gee, when Becky said you are in charge of the brag basket she probably didnt mean just me. What wonderful things have yall been doing this week?. ...
ADP-ribosylation factor, GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the ...
ADP-ribosylation factor (ARF) proteins and inhibitory peptides derived from ARFs have demonstrated activities in a number of in vitro assays that measure ER-to-Golgi and intra-Golgi transport and endosome fusion. To better understand the roles of ARF proteins in vivo, stable cell lines were obtained from normal rat kidney (NRK) cells transfected with either wild-type or a dominant activating allele ([Q71L]) of the human ARF1 gene under the control of the interferon-inducible mouse Mx1 promoter. Upon addition of interferon, expression of ARF1 proteins increased with a half-time of 7-8 h, as determined by immunoblot analysis. Induction of mutant ARF1, but not wild-type ARF1, led to an inhibition of protein secretion with kinetics similar to that observed for induction of protein expression. Examination of the Golgi apparatus and the ER by indirect immunofluorescence or transmission electron microscopy revealed that expression of low levels of mutant ARF1 protein correlated with a dramatic increase ...
FUNCTION: Guanine nucleotide-exchange factor (GEF) required for the formation or budding of transport vesicles from the ER. This function involves the cytoplasmic domain of the protein, which is thought to interact with the small GTP-binding protein SAR1. Required for autophagy. MISCELLANEOUS: In the process of transport, SEC12 itself may migrate to the Golgi apparatus and function in subsequent transport events. MISCELLANEOUS: Present with 6160 molecules/cell in log phase SD medium ...
In eukaryotes, calcium signalling has been linked to hydrolysis of the phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P(2)). The final enzyme in the synthesis of this phosphoinositide, a Type I phosphatidylinositol 4-phosphate 5-kinase (PIP5K), is activated by the small G protein ADP-ribosylation factor 1 (ARF1). In mammals, the ARF-PIP5K pathway is a key regulator of cell motility, secretion and cell signalling. We report the characterisation of a unique, putative bifunctional PIP5K in the human malaria parasite Plasmodium falciparum. The protein comprises a C-terminal, functional PIP5K domain with catalytic specificity for phosphatidylinositol 4-phosphate. The recombinant enzyme is activated by ARF1 but not phosphatidic acid. The protein also incorporates an unusual N-terminal domain with potential helix-loop-helix EF-hand-like motifs that is a member of the neuronal calcium sensor family (NCS). Intriguingly, NCS-1 has been shown to stimulate phosphatidylinositol 4-phosphate synthesis by ...
Ich möchte nicht herumprahlen , aber ich habe mir mal ein sehr besonderes Bad eingelassen I dont mean to brag , but Im the greatest. I dont mean to brag , but Ive been handling this account on my own since I brought it in.. Ich habe diesen Kunden allein betreut , seit ich ihn bekommen habe. Inhalt möglicherweise unpassend Entsperren.. Ein Beispiel vorschlagen. Synonyme Konjugation Reverso Corporate. These examples are not to brag but to share what the possibilities are.. I tell you this by way of example, and certainly not to brag. Not to brag or anything, but I do have a couple of ladies into me, and one of them is an Orc chiefs daughter.. The Prime Minister likes to brag because he has never had ministers resign. We know the Liberals love to brag. Businesses like to brag in their advertising about quality of work, commitment to their customers, and excellent service.. Pop wasnt one to brag. And although I clearly do, I hate to brag. Six seconds, not to brag. Six secondes, sans me ...
Arginine adenosine-5′-diphosphoribosylation (ADP-ribosylation) is an enzyme-catalyzed potentially reversible posttranslational changes where the ADP-ribose moiety is transferred from NAD+ towards the guanidino CHR2797 moiety of arginine. proteins with binding companions e.g. toxin-catalyzed ADP-ribosylation of actin at R177 blocks actin polymerization sterically. In case there is the nucleotide-gated P2X7 ion route ADP-ribosylation at R125 near the ligand-binding site causes route Rabbit Polyclonal to SF3B3. gating. Arginine-specific ADP-ribosyltransferases (ARTs) bring a quality R-S-EXE theme that distinguishes these enzymes from structurally related enzymes which catalyze ADP-ribosylation of additional amino acid part chains DNA or little substances. Arginine-specific ADP-ribosylation could be inhibited by little molecule arginine analogues such as for example CHR2797 agmatine or meta-iodobenzylguanidine (MIBG) which themselves can serve as focuses on for arginine-specific ARTs. ...
ARF proteins, which mediate vesicular transport, have little or no intrinsic GTPase activity. They rely on the actions of GTPase-activating proteins (GAPs) for their function. The in vitro GTPase activity of the Saccharomyces cerevisiae ARF proteins Arf1 and Arf2 is stimulated by the yeast Gcs1 protein, and in vivo genetic interactions between arf and gcs1 mutations implicate Gcs1 in vesicular transport. However, the Gcs1 protein is dispensable, indicating that additional ARF GAP proteins exist. We show that the structurally related protein Glo3, which is also dispensable, also exhibits ARF GAP activity. Genetic and in vitro approaches reveal that Glo3 and Gcs1 have an overlapping essential function at the endoplasmic reticulum (ER)-Golgi stage of vesicular transport. Mutant cells deficient for both ARF GAPs cannot proliferate, undergo a dramatic accumulation of ER and are defective for protein transport between ER and Golgi. The glo3Delta and gcs1Delta single mutations each interact with a ...
Summary Arl (Arf-like) proteins, GTP-binding proteins of the Ras superfamily, are molecular switches that cycle between a GDP-bound inactive and GTP-bound active state. There are 16 members of the Arl subfamily in the human genome whose basic mechanistic function is unknown. The interactome of Arl2/3 includes proteins involved in retinopathies and other ciliary diseases such as Leber¿s Congenital Amaurosis (LCA) and kidney diseases such as nephronophthisis. Arl6 has been found mutated in Bardet Biedl Syndrome, another pleiotropic ciliary disease. In the proposed interdisciplinary project I want to explore the function of the protein network of Arl2/3 and Arl6 by a combination of biochemical, biophysical and structural methods and use the knowledge obtained to probe their function in live cells. As with other subfamily proteins of the Ras superfamily which have been found to mediate similar biological functions I want to derive a basic understanding of the function of Arl proteins and how it ...
ADP ribosylation factor 1 (Arf1). Arl1 subfamily. Arl1 (Arf-like 1) localizes to the Golgi complex, where it is believed to recruit effector proteins to the trans-Golgi network. Like most members of the Arf family, Arl1 is myristoylated at its N-terminal helix and mutation of the myristoylation site disrupts Golgi targeting. In humans, the Golgi-localized proteins golgin-97 and golgin-245 have been identified as Arl1 effectors. Golgins are large coiled-coil proteins found in the Golgi, and these golgins contain a C-terminal GRIP domain, which is the site of Arl1 binding. Additional Arl1 effectors include the GARP (Golgi-associated retrograde protein)/VFT (Vps53) vesicle-tethering complex and Arfaptin 2. Arl1 is not required for exocytosis, but appears necessary for trafficking from the endosomes to the Golgi. In Drosophila zygotes, mutation of Arl1 is lethal, and in the host-bloodstream form of Trypanosoma brucei, Arl1 is essential for viability. ...
TY - JOUR. T1 - Characterization of a rat brain phospholipase D isozyme. AU - Min, Do Sik. AU - Park, Seung Kiel. AU - Exton, John H.. PY - 1998/3/20. Y1 - 1998/3/20. N2 - We have recently cloned a cDNA encoding a phospholipase D (PLD) from rat brain and named it rPLD1. It shows 90% amino acid identity with the human PLD isoform hPLD1b. We have expressed rPLD1 as a histidine-tagged fusion protein in insect (Sf9) cells using the expression vector pBlueBacHis and purified the recombinant protein to homogeneity by Ni2+-agarose affinity chromatography. Phosphatidylinositol 4,5-P2 and phosphatidylinositol 3,4,5- P3 activated the PLD equipotently, but other acidic phospholipids were ineffective. The activity of rPLD1 was dependent on both Mg2+ and Ca2+. It was specific for phosphatidylcholine and showed a broad dependence on pH with optimum activity at pH 6.5-7.5. The enzyme was inhibited by oleate and activated by the small G proteins ARF3 and RhoA in the presence of guanosine ...
GTP-binding protein involved in protein trafficking that regulates endocytic recycling and cytoskeleton remodeling. Required for normal completion of mitotic cytokinesis. May also modulate vesicle budding and uncoating within the Golgi apparatus. Involved in the regulation of dendritic spine development, contributing to the regulation of dendritic branching and filopodia extension (PubMed:16672654). Involved in epithelial polarization (By similarity).
Hong Xu wrote: , , Dear Colleagues: , , Our group is using the Clontech s HeLa cDNA library in a yeast , two-hybrid screening with the bait fused to the GAL4AD and the yeast , strain CG 1945. Many of the clones sequenced show one of the following , sequences, including ITBA2 (whatever it is, since almost no references , can be found in Medline), ribosomal s20, transketolase, Ring12 (probably , a proteasome subunit), ADP ribosylation factor, etc., with those listed , above showing up most frequently. We would like to know whether or not , these factors have also been found in somebody else two-hybrid , screenings using the same Clontech HeLa library. , , You are welcome to post your answer to our question in this newsgroup. , Or you can call me at 973-781-7987. , , Thank you very much. , , Richard Dear Richard, try this URL and you find an overview of the most common trash found in two hybrid screens: http://www.fccc.edu/research/labs/golemis/main_false.html Hope this helps, Ricky ...
SWISS-MODEL Template Library (SMTL) entry for 4ylg.1. Structure of an ADP ribosylation factor from Entamoeba histolytica HM-1:IMSS bound to Mg-GDP
This gene encodes a phosphoinositide binding protein containing ARF-GAP, RHO-GAP, RAS-associating, and pleckstrin homology domains. The ARF-GAP and RHO-GAP domains cooperate in mediating rearrangements in the cell cytoskeleton and cell shape. It is a specific PtdIns(3,4,5)P3/PtdIns(3,4)P2-stimulated Arf6-GAP protein. An alternatively spliced transcript has been found for this gene, but its biological validity has not been determined ...
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Stimulates the exchange of guanyl nucleotides associated with the GTPase ARF. Under normal cellular physiological conditions, the concentration of GTP is higher than that of GDP, favoring the replacement of GDP by GTP in association with the GTPase ...
Human ARL1 partial ORF ( NP_001168, 72 a.a. - 181 a.a.) recombinant protein with GST-tag at N-terminal. (H00000400-Q01) - Products - Abnova
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said: u guys r so Imature. I believe her 2 i bet EVERYONE here that DOESNT believe her would fangirl and they r just NORMAL boys thats it. Just treat them like normal people they shouldnt be treated ANY different then us. I bet if u asked them they would say that they wanna be treated like normal people. And maybe if u go 2 a concert u might get there # and then u would wanna brag about it i know i would. So keep ur nasty comments 2 ur self. Just leave her alone! I want there #s 2 but she probably wont give it 2 me(i REALLY want it) but keep ur nasty comments 2 ur selves i bet if ANYONE from one direction saw these comments they wouldnt be happy with these comments except the nice ones ...
No. You are not. But, were odd like that. We also yell, No one expects the Spanish Inquisition! whenever anybody says that they didnt expect something. Im not sure these are things to brag about.. ...
Those who know me know I take every opportunity to brag on my family. This is the stuff of life; people are what really matter, and I could not be mor
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ADP-ribosylation factor regulates hPLD2". The Journal of Biological Chemistry. 273 (21): 12846-52. doi:10.1074/jbc.273.21.12846 ... "Cardiac phospholipase D2 localizes to sarcolemmal membranes and is inhibited by alpha-actinin in an ADP-ribosylation factor- ... "Cardiac phospholipase D2 localizes to sarcolemmal membranes and is inhibited by alpha-actinin in an ADP-ribosylation factor- ... May 2003). "The direct interaction of phospholipase C-gamma 1 with phospholipase D2 is important for epidermal growth factor ...
ADP-ribosylation factor 1 is a protein that in humans is encoded by the ARF1 gene. ADP-ribosylation factor 1 (ARF1) is a member ... Frank S, Upender S, Hansen SH, Casanova JE (1998). "ARNO is a guanine nucleotide exchange factor for ADP-ribosylation factor 6 ... ARF1 ADP-ribosylation factor 1". Mitchell R, Robertson DN, Holland PJ, Collins D, Lutz EM, Johnson MS (September 2003). "ADP- ... 1991). "ADP-ribosylation factor is a subunit of the coat of Golgi-derived COP-coated vesicles: a novel role for a GTP-binding ...
December 1995). "Human ADP-ribosylation factor-activated phosphatidylcholine-specific phospholipase D defines a new and highly ... July 1998). "Activation of phospholipase D1 by direct interaction with ADP-ribosylation factor 1 and RalA". FEBS Letters. 430 ( ... July 1998). "Activation of phospholipase D1 by direct interaction with ADP-ribosylation factor 1 and RalA". FEBS Letters. 430 ( ... Activation of the purified enzymes by phosphatidylinositol 4,5-bisphosphate, ADP-ribosylation factor, and Rho family monomeric ...
ADP-ribosylation factor 6 (ARF6) is a member of the ADP ribosylation factor family of GTP-binding proteins. ARF6 has a variety ... Frank S, Upender S, Hansen SH, Casanova JE (1998). "ARNO is a guanine nucleotide exchange factor for ADP-ribosylation factor 6 ... "ADP-ribosylation factor 6 as a target of guanine nucleotide exchange factor GRP1". J. Biol. Chem. 274 (38): 27099-104. doi: ... "Entrez Gene: ARF6 ADP-ribosylation factor 6". Claing A, Chen W, Miller WE, Vitale N, Moss J, Premont RT, Lefkowitz RJ (November ...
ADP-ribosylation factor-like protein 13B (ARL13B), also known as ADP-ribosylation factor-like protein 2-like 1, is a protein ... "Entrez Gene: ADP-ribosylation factor-like 13B". Cantagrel V, Silhavy JL, Bielas SL, Swistun D, Marsh SE, Bertrand JY, Audollent ... This gene encodes a member of the ADP-ribosylation factor-like family. The encoded protein is a small GTPase that contains both ...
ADP-ribosylation factor 4 is a protein that in humans is encoded by the ARF4 gene. ADP-ribosylation factor 4 (ARF4) is a member ... ARF4 ADP-ribosylation factor 4". Kim SW, Hayashi M, Lo JF, Yang Y, Yoo JS, Lee JD (Jan 2003). "ADP-ribosylation factor 4 small ... "ADP-ribosylation factor 4 small GTPase mediates epidermal growth factor receptor-dependent phospholipase D2 activation". J. ... Kahn RA, Kern FG, Clark J, Gelmann EP, Rulka C (1991). "Human ADP-ribosylation factors. A functionally conserved family of GTP- ...
ADP-ribosylation factor-like 15 is a protein in humans that is encoded by the ARL15 gene. GRCh38: Ensembl release 89: ... "Entrez Gene: ADP-ribosylation factor-like 15". Retrieved 2013-03-16. CS1 maint: discouraged parameter (link) v t e. ...
ADP-ribosylation factor 3 is a protein that in humans is encoded by the ARF3 gene. ADP-ribosylation factor 3 (ARF3) is a member ... "Entrez Gene: ARF3 ADP-ribosylation factor 3". Kanoh H, Williger BT, Exton JH (February 1997). "Arfaptin 1, a putative cytosolic ... Williger BT, Provost JJ, Ho WT, Milstine J, Exton JH (July 1999). "Arfaptin 1 forms a complex with ADP-ribosylation factor and ... Lee CM, Haun RS, Tsai SC, Moss J, Vaughan M (1992). "Characterization of the human gene encoding ADP-ribosylation factor 1, a ...
ADP-ribosylation factor-like protein 8A is a protein that in humans is encoded by the ARL8A gene. GRCh38: Ensembl release 89: ... "Entrez Gene: ARL8A ADP-ribosylation factor-like 8A". Human ARL8A genome location and ARL8A gene details page in the UCSC Genome ...
ADP-ribosylation factor-like protein 8B is a protein that in humans is encoded by the ARL8B gene. GRCh38: Ensembl release 89: ... "Entrez Gene: ARL8B ADP-ribosylation factor-like 8B". CS1 maint: discouraged parameter (link) Human ARL8B genome location and ...
ADP-ribosylation factor-like protein 4A is a protein that in humans is encoded by the ARL4A gene. ADP-ribosylation factor-like ... "Entrez Gene: ARL4A ADP-ribosylation factor-like 4A". CS1 maint: discouraged parameter (link) Lin CY, Huang PH, Liao WL, Cheng ... Jacobs S, Schilf C, Fliegert F, Koling S, Weber Y, Schürmann A, Joost HG (1999). "ADP-ribosylation factor (ARF)-like 4, 6, and ... 4A is a member of the ADP-ribosylation factor family of GTP-binding proteins. ARL4A is similar to ARL4C and ARL4D and each has ...
ADP-ribosylation factor-like protein 4D is a protein that in humans is encoded by the ARL4D gene. ADP-ribosylation factor 4D is ... "Entrez Gene: ARL4D ADP-ribosylation factor-like 4D". "DEXA data for Arl4d". Wellcome Trust Sanger Institute. "Radiography data ... Jacobs S, Schilf C, Fliegert F, Koling S, Weber Y, Schürmann A, Joost HG (Aug 1999). "ADP-ribosylation factor (ARF)-like 4, 6, ... "Recognition of ADP-ribosylation factor 4-like by HLA-A2-restricted and tumor-reactive cytotoxic T lymphocytes from patients ...
ADP-ribosylation factor-like) family of proteins, which are structurally related to ADP-ribosylation factors (ARFs). ARFs, ... ADP-ribosylation factor-like protein 1 is a protein that in humans is encoded by the ARL1 gene. The protein encoded by this ... "Entrez Gene: ARL1 ADP-ribosylation factor-like 1". CS1 maint: discouraged parameter (link) Lu L, Hong W (September 2003). " ... Van Valkenburgh H, Shern JF, Sharer JD, Zhu X, Kahn RA (June 2001). "ADP-ribosylation factors (ARFs) and ARF-like 1 (ARL1) have ...
ADP-ribosylation factor-like protein 3 is a protein that in humans is encoded by the ARL3 gene. ADP-ribosylation factor-like 3 ... "Entrez Gene: ARL3 ADP-ribosylation factor-like 3". Stelzl U, Worm U, Lalowski M, Haenig C, Brembeck FH, Goehler H, Stroedicke M ... Van Valkenburgh H, Shern JF, Sharer JD, Zhu X, Kahn RA (June 2001). "ADP-ribosylation factors (ARFs) and ARF-like 1 (ARL1) have ... Kim HS (1998). "Assignment of the human ADP-ribosylation factor-like 3 (ARL3) gene to chromosome 10 band q23.3 by radiation ...
ADP-ribosylation factor-like protein 6 is a protein that in humans is encoded by the ARL6 gene. The protein encoded by this ... "Entrez Gene: ARL6 ADP-ribosylation factor-like 6". GeneReviews/NIH/NCBI/UW entry on Bardet-Biedl Syndrome Human ARL6 genome ... 1999). "ADP-ribosylation factor (ARF)-like 4, 6, and 7 represent a subgroup of the ARF family characterization by rapid ... 1999). "A novel ADP-ribosylation like factor (ARL-6), interacts with the protein-conducting channel SEC61beta subunit". FEBS ...
ADP-ribosylation factor-related protein 1 is a protein that in humans is encoded by the ARFRP1 gene. The protein encoded by ... "Entrez Gene: ARFRP1 ADP-ribosylation factor related protein 1". Schürmann A, Schmidt M, Asmus M, Bayer S, Fliegert F, Koling S ... It is related to the ADP-ribosylation factor (ARF) and ARF-like (ARL) genes. The gene is located in a gene cluster that ... 1999). "The ADP-ribosylation factor (ARF)-related GTPase ARF-related protein binds to the ARF-specific guanine nucleotide ...
ADP ribosylation factor like GTPase 6 interacting protein 6 is a protein that in the humans is encoded by the ARL6IP6 gene. It ... "ARL6IP6 ADP ribosylation factor like GTPase 6 interacting protein 6 [Homo sapiens (human)] - Gene - NCBI". www.ncbi.nlm.nih.gov ... "ADP ribosylation factor like GTPase 6 interacting protein 6". NCBI Gene. "UniProtKB - Q8N6S5 (AR6P6_HUMAN)". uniprot.org. ...
"ARL14 ADP-ribosylation factor-like 14 [Homo sapiens (human)". Entrez Gene. "ARL14EP ADP-ribosylation factor-like 14 effector ... PSD4 as a guanine exchange factor, loads ARL14/ARF7 with GTP. Subsequently, ARF7EP interacts with MYO1E which binds itself to ... PSD4 (Pleckstrin and Sec7 Domain containing 4) is a GEF (Guanine nucleotide Exchange Factor) that loads ARL14/ARF7 with GTP. ... is due to mutations in the genes that code for transcription factors that regulate the expression of the MHC class II genes. It ...
Premont RT, Claing A, Vitale N, Perry SJ, Lefkowitz RJ (July 2000). "The GIT family of ADP-ribosylation factor GTPase- ... a G protein-coupled receptor kinase-associated ADP ribosylation factor GTPase-activating protein". Proc. Natl. Acad. Sci. U.S.A ... receptor kinase 2 in a signaling unit that regulates p42/p44 mitogen-activated protein kinase by epidermal growth factor". J. ...
Premont RT, Claing A, Vitale N, Perry SJ, Lefkowitz RJ (Jul 2000). "The GIT family of ADP-ribosylation factor GTPase-activating ... GIT proteins interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating ... "An ADP-ribosylation factor GTPase-activating protein Git2-short/KIAA0148 is involved in subcellular localization of paxillin ... a G protein-coupled receptor kinase-associated ADP ribosylation factor GTPase-activating protein". Proceedings of the National ...
BFA blocks the activation of some ADP-ribosylation factors (ARFs). ARFs are small GTPases which regulate vesicular trafficking ... BFA inhibits the function of several guanine nucleotide exchange factors (GEFs) that mediate GTP-binding of ARFs. Treatment of ...
ADP-ribosylation factor GTPase-activating protein 3 is a protein that in humans is encoded by the ARFGAP3 gene. The protein ... "Entrez Gene: ARFGAP3 ADP-ribosylation factor GTPase activating protein 3". Human ARFGAP1 genome location and ARFGAP1 gene ... which associates with the Golgi apparatus and which is thought to interact with ADP-ribosylation factor 1 (ARF1). The encoded ...
Premont RT, Claing A, Vitale N, Perry SJ, Lefkowitz RJ (2000). "The GIT family of ADP-ribosylation factor GTPase-activating ... Shin EY, Woo KN, Lee CS, Koo SH, Kim YG, Kim WJ, Bae CD, Chang SI, Kim EG (2004). "Basic fibroblast growth factor stimulates ... Rho guanine nucleotide exchange factor 7 is a protein that in humans is encoded by the ARHGEF7 gene. ARHGEF7 is commonly known ... βPIX contains a central DH/PH RhoGEF domain that functions as a guanine nucleotide exchange factor (GEF) for small GTPases of ...
Premont RT, Claing A, Vitale N, Perry SJ, Lefkowitz RJ (August 2000). "The GIT family of ADP-ribosylation factor GTPase- ... a G protein-coupled receptor kinase-associated ADP ribosylation factor GTPase-activating protein". Proc Natl Acad Sci U S A. 95 ... Zhao ZS, Manser E, Loo TH, Lim L (September 2000). "Coupling of PAK-interacting exchange factor PIX to GIT1 promotes focal ... Zhao ZS, Manser E, Loo TH, Lim L (2000). "Coupling of PAK-interacting exchange factor PIX to GIT1 promotes focal complex ...
"Entrez Gene: ARL6IP5 ADP-ribosylation-like factor 6 interacting protein 5". Human ARL6IP5 genome location and ARL6IP5 gene ... 1999). "A novel ADP-ribosylation like factor (ARL-6), interacts with the protein-conducting channel SEC61beta subunit". FEBS ...
"Entrez Gene: ARFIP2 ADP-ribosylation factor interacting protein 2 (arfaptin 2)". D'Souza-Schorey C, Boshans RL, McDonough M, ... Kanoh H, Williger BT, Exton JH (February 1997). "Arfaptin 1, a putative cytosolic target protein of ADP-ribosylation factor, is ... Shin OH, Exton JH (August 2001). "Differential binding of arfaptin 2/POR1 to ADP-ribosylation factors and Rac1". Biochemical ... Shin OH, Exton JH (August 2001). "Differential binding of arfaptin 2/POR1 to ADP-ribosylation factors and Rac1". Biochemical ...
ADP-ribosylation factor-like protein 6-interacting protein 1 is a protein that in humans is encoded by the ARL6IP1 gene. GRCh38 ... "Entrez Gene: ARL6IP1 ADP-ribosylation factor-like 6 interacting protein 1". Human ARL6IP1 genome location and ARL6IP1 gene ... "A novel ADP-ribosylation like factor (ARL-6), interacts with the protein-conducting channel SEC61beta subunit". FEBS Lett. 459 ... ADP-ribosylation-like factor-6 interacting protein (ARL6)". Genomics. 68 (3): 351-4. doi:10.1006/geno.2000.6278. PMID 10995579 ...
2000). "The GIT family of ADP-ribosylation factor GTPase-activating proteins. Functional diversity of GIT2 through alternative ... αPIX contains a central DH/PH RhoGEF domain that functions as a guanine nucleotide exchange factor (GEF) for small GTPases of ... Rho guanine nucleotide exchange factor 6 is a protein that, in humans, is encoded by the ARHGEF6 gene. ARHGEF6 is commonly ... "Entrez Gene: ARHGEF6 Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6". Zhou W, Li X, Premont RT (May 2016). "Expanding ...
ADP ribosylation factor (ARF) is a GTPase involved in membrane traffic. There are 6 mammalian ARFs which are regulated by over ... Coat protein, or COPI, is an ADP ribosylation factor (ARF)-dependent protein involved in membrane traffic. COPI was first ... "ADP-ribosylation factor is a subunit of the coat of Golgi-derived COP-coated vesicles: a novel role for a GTP-binding protein ... This receptor then binds to an ARF-GEF, a class of guanine nucleotide exchange factors. This protein in turn binds to the ARF. ...
"Entrez Gene: ARFIP1 ADP-ribosylation factor interacting protein 1 (arfaptin 1)". Tsai SC, Adamik R, Hong JX, et al. (1998). " ... Williger BT, Provost JJ, Ho WT, Milstine J, Exton JH (Aug 1999). "Arfaptin 1 forms a complex with ADP-ribosylation factor and ... Kanoh H, Williger BT, Exton JH (Apr 1997). "Arfaptin 1, a putative cytosolic target protein of ADP-ribosylation factor, is ... Ho WT, Exton JH, Williger BT (2003). "Arfaptin 1 inhibits ADP-ribosylation factor-dependent matrix metalloproteinase-9 ...
ARL6IP1: encoding protein ADP-ribosylation factor-like protein 6-interacting protein 1 ... PMFBP1: encoding protein Polyamine-modulated factor 1-binding protein 1. *POLR3K: encoding enzyme DNA-directed RNA polymerase ...
... and ADP-ribosylation. This affects their function of gene regulation. In general, genes that are active have less bound histone ... SBF is a transcription factor that is activated in late G1 phase, when it dissociates from its repressor Whi5. This occurs when ... and ADP-ribosylation. The core of the histones H2A and H2B can also be modified. Combinations of modifications are thought to ... The serotonylation potentiates the binding of the general transcription factor TFIID to the TATA box.[49] ...
In addition, the inactivation of Rho protein by ADP-ribosylation in Rac1 microinjection reduced the formation of actin stress ... This led to the conclusion that CNK1 couples specific Rho exchange factors to the JNK MAP kinase pathway, providing specificity ... The inhibition of Rho by C3 transferase ribosylation resulted in an inhibition of focal adhesion and stress fibre assembly, but ... Indeed, Rho-dependent response is stimulated by the action of growth factors on Rac protein.[8] ...
ADP-ribosyl)ation directs recruitment and activation of an ATP-dependent chromatin remodeler". Proc. Natl. Acad. Sci. U.S.A. ... "Epigenetic factors in cancer risk: effect of chemical carcinogens on global DNA methylation pattern in human TK6 cells". PLoS ... ADP)-ribose polymerase) and its product poly(ADP)-ribose (PAR) accumulate at sites of DNA damage as part of a repair process.[ ... Malanga M, Althaus FR (2005). "The role of poly(ADP-ribose) in the DNA damage signaling network". Biochem Cell Biol. 83 (3): ...
... and the A1 subunit is freed to bind with a human partner protein called ADP-ribosylation factor 6 (Arf6).[29] Binding exposes ... The A1 portion of the A subunit is an enzyme that ADP-ribosylates G proteins, while the A2 chain fits into the central pore of ... "Sources of Infection & Risk Factors". Centers for Disease Control and Prevention. November 7, 2014. Archived from the original ... Risk factors for the disease include poor sanitation, not enough clean drinking water, and poverty.[2] There are concerns that ...
ADP-ribosylation, and ubiquitination have also been reported.[3][22] These combinations of different covalent modifications on ... and the general transcription factors TFIIE and TFIIF.[4] Other substrates include the transcription factors Sp1, KLF5, FOXO1, ... MORF (MOZ-related factor) exhibits very close homology to MOZ throughout its entire length.[4] It contains an N-terminal ... TAFII250 is one of the TBP-associated factor subunits of TFIID, and it shares a Gly-X-Gly pattern with Gcn5 that is important ...
... to arginine or other guanidine compounds by utilizing ADP-ribosylation factors (ARFs). In the absence of arginine or simple ... Locht, C; Antoine, R (1995). "A proposed mechanism of ADP-ribosylation catalyzed by the pertussis toxin S1 subunit". Biochimie ... the A1 domain unfolds and is redirected back to the cytosol where it refolds and catalyzes ADP-ribosylation of certain G ... This is accomplished through fusion of epidermal growth factor(EGF) with SubAB's A subunit. Cancer cells that express receptors ...
ADP-ribosylation)". Nucleic Acids Research 19 (21): 5943-7. PMC 329051. PMID 1945881. doi:10.1093/nar/19.21.5943.. ... "Activation of the abundant nuclear factor poly(ADP-ribose) polymerase-1 by Helicobacter pylori". Proceedings of the National ... A PARP1 ou poli(ADP-ribosa) polimerase 1, tamén chamada NAD+ ADP-ribosiltransferase 1 ou poli(ADP-ribosa) sintase 1 é un encima ... ADP-ribosyl)ation develop normally but are susceptible to skin disease". Genes & Development 9 (5): 509-20. PMID 7698643. doi: ...
"ARNO is a guanine nucleotide exchange factor for ADP-ribosylation factor 6". J. Biol. Chem. 273 (1): 23-7. 1998. doi:10.1074/ ... "ADP-ribosylation factor is functionally and physically associated with the Golgi complex". Proc. Natl. Acad. Sci. U.S.A. 87 (3 ... ARF1, ADP ribosylation factor 1, PVNH8. معرفات خارجية. الوراثة المندلية البشرية عبر الإنترنت 103180 MGI: MGI:99431 HomoloGene: ... ARF1‏ (ADP ribosylation factor 1) هوَ بروتين يُشَفر بواسطة جين ARF1 في الإنسان.[1] ...
Proline hydroxylation is also a vital component of hypoxia response via hypoxia inducible factors. In some cases, proline may ... ADP-ribosylation. *Deamination. *Oxidative deamination to aldehyde. *O-glycosylation. *Imine formation. *Glycation ...
Corda D, Di Girolamo M «Functional aspects of protein mono-ADP-ribosylation». EMBO J., 22, 9, 2003, pàg. 1953-8. DOI: 10.1093/ ... The isolation and identification of the anti-black tongue factor» (PDF). J. Biol. Chem., 123, 1, 1938, pàg. 137-49. ... La poli-ADP-ribosilació té lloc gràcies a l'acció de les poli(ADP-ribosa) polimerases.[53][50] L'estructura poli(ADP-ribosa) ... que s'anomena poli-ADP-ribosilació.[50] La mono-ADP-ribosliació es va identificar per primera vegada en el mecanisme d'un ...
... such as the ADP-ribosylation factor or ARF family of small GTP-binding proteins that are involved in vesicle-mediated transport ... Translocation factors[edit]. For a discussion of Translocation factors and the role of GTP, see signal recognition particle ( ... Translation factor family[edit]. Multiple translation factor family GTPases play important roles in initiation, elongation and ... Kahn, RA; Gilman, AG (1986). "The protein cofactor necessary for ADP-ribosylation of Gs by cholera toxin is itself a GTP ...
nucleotide addition such as ADP-ribosylation. *phosphate ester (O-linked) or phosphoramidate (N-linked) formation * ... beta-Lysine addition on a conserved lysine of the elongation factor P (EFP) in most bacteria.[9] EFP is an homolog to eIF5A ( ... Modifications of translation factorsEdit. *diphthamide formation (on a histidine found in eEF2) ... 1989). "Murine elongation factor 1 alpha (EF-1 alpha) is posttranslationally modified by novel amide-linked ethanolamine- ...
"Functional aspects of protein mono-ADP-ribosylation". EMBO J. 22 (9): 1953-8. PMC 156081. PMID 12727863. doi:10.1093/emboj/ ... Chamaron cofermento ao factor non identificado responsable deste efecto. A través dunha longa e dificultosa purificación a ... A ADP-ribosilación implica quer a adición dun só grupo ADP-ribosa (mono-ADP-ribosilación) quer a transferencia de ADP-ribosa ás ... ADP-ribosil)ación lévana a cabo as poli(ADP-ribosa) polimerases.[53][56] A estrutura da poli-(ADP-ribosa) está implicada na ...
Toxin B (cytotoxin) induces actin depolymerization by a mechanism correlated with a decrease in the ADP-ribosylation of the low ... Risk factors[edit]. Antibiotics[edit]. C. difficile colitis is associated most strongly with the use of these antibiotics: ... Risk factors. Antibiotics, proton pump inhibitors, hospitalization, other health problems, older age[1]. ... Long-term hospitalization or residence in a nursing home within the previous year are independent risk factors for increased ...
... dependent ADP-ribosylation of elongation factor 2, thereby inhibiting protein synthesis in eukaryotic cells. Fragment B binds ... C. diphtheriae produces diphtheria toxin which alters protein function in the host by inactivating the elongation factor EF-2. ...
It acts similarly to the cholera toxin by raising cAMP levels through ADP-ribosylation of the alpha-subunit of a Gs protein ... factor. *Clumping factor A. *Fibronectin binding protein A. Mycotoxins. *Aflatoxin. *Amatoxin (alpha-amanitin, beta-amanitin, ...
D-glucose + ATP → D-glucose-6-phosphate + ADP. ΔG° = −16.7 kJ/mol (° indicates measurement at standard condition). Researcher D ... identify dynamic changes in the phosphorylation status of more than 6000 sites after stimulation with epidermal growth factor.[ ... is synthesized in the mitochondrion by addition of a third phosphate group to ADP in a process referred to as oxidative ... ADP-ribosylation. *Deamination. *Oxidative deamination to aldehyde. *O-glycosylation. *Imine formation. *Glycation ...
ADP-ribosyl)ation directs recruitment and activation of an ATP-dependent chromatin remodeler". Proc. Natl. Acad. Sci. U.S.A. ... Such effects on cellular and physiological phenotypic traits may result from external or environmental factors, or be part of ... ADP)-ribose polymerase) and its product poly(ADP)-ribose (PAR) accumulate at sites of DNA damage as part of a repair process.[ ... The yeast prion PSI is generated by a conformational change of a translation termination factor, which is then inherited by ...
regulation of protein ADP-ribosylation. • positive regulation of protein complex assembly. • positive regulation of protein ... Joseph AM, Kumar M, Mitra D (2005). "Nef: "necessary and enforcing factor" in HIV infection". Curr. HIV Res. 3 (1): 87-94. doi: ... positive regulation of tumor necrosis factor production. • CD8-positive, alpha-beta T cell differentiation involved in immune ... positive regulation of tumor necrosis factor biosynthetic process. • positive regulation of glycolytic process. • astrocyte ...
Shin OH, Exton JH (Aug 2001). "Differential binding of arfaptin 2/POR1 to ADP-ribosylation factors and Rac1". Biochemical and ... hepatocyte growth factor receptor signaling pathway. • regulation of stress fiber assembly. • positive regulation of protein ... vascular endothelial growth factor receptor signaling pathway. • substrate adhesion-dependent cell spreading. • cell ... of rac1 specifies interaction with a subset of guanine nucleotide exchange factors". The Journal of Biological Chemistry. 276 ( ...
Molecular characterization of the GTPase-activating domain of ADP-ribosylation factor domain protein 1 (ARD1)". J. Biol. Chem. ...
ADP-ribosylation involves either the addition of a single ADP-ribose moiety, in mono-ADP-ribosylation, or the transferral of ... In bacteriology, NAD, sometimes referred to factor V, is used a supplement to culture media for some fastidious bacteria.[90] ... ADP-ribose to proteins in long branched chains, which is called poly(ADP-ribosyl)ation.[54] Mono-ADP-ribosylation was first ... ADP-ribosyl)ation is carried out by the poly(ADP-ribose) polymerases.[54][57] The poly(ADP-ribose) structure is involved in the ...
CTA1 is then free to bind with a human partner protein called ADP-ribosylation factor 6 (Arf6); binding to Arf6 drives a change ... The CTA1 fragment catalyses ADP-ribosylation of the Gs alpha subunit (Gαs) proteins using NAD. The ADP-ribosylation causes the ... The A1 portion of the chain (CTA1) is a globular enzyme payload that ADP-ribosylates G proteins, while the A2 chain (CTA2) ... produced by Bordetella pertussis acts in a similar manner with the exception that it ADP-ribosylates the Gαi subunit, rendering ...
ADP ribosylation factor)(ADP ribosylation factors)結合,從而參與啟動囊泡的逆向轉運[11][12][13]。同時又發現COPγ、COPζ各有2種亞型,即γ1、γ2、ζ1、ζ2[14],這些亞型間的交叉組合 ... 循環細胞膜囊泡(Tissue factor-carrying microvesicles、TF-MV):是一種細胞在激活、受損或凋亡時從細胞膜上釋放的脂質囊泡(直徑約0.1~1 μm),具有促凝活性的TF-MV最主要來源於動脈粥樣硬化斑塊[35][36] ... Coronary no
"Activation of phospholipase D1 by direct interaction with ADP-ribosylation factor 1 and RalA". FEBS Letters. 430 (3): 231-5. ... "Activation of phospholipase D1 by direct interaction with ADP-ribosylation factor 1 and RalA". FEBS Letters. 430 (3): 231-5. ... a pleckstrin homology domain containing guanine nucleotide exchange factor for Ral". The Journal of Biological Chemistry. 275 ( ...
bHLH transcription factor binding. • NAD+ binding. • identical protein binding. • NAD+ ADP-ribosyltransferase activity. • ... protein ADP-ribosylation. • leptin-mediated signaling pathway. • negative regulation of apoptotic process. • negative ... cellular response to leukemia inhibitory factor. • positive regulation of angiogenesis. • transforming growth factor beta ... They add the acetyl group from the protein to the ADP-ribose component of NAD+ to form O-acetyl-ADP-ribose. The HDAC activity ...
A protein coat assembles and disassembles due to an ADP ribosylation factor (ARF) protein. ... carrying and transmitting virulence factors into host cells and modulating host defense and response.[14] ...
"Function of BRCA1 in the DNA damage response is mediated by ADP-ribosylation". Cancer Cell. 23 (5): 693-704. doi:10.1016/j.ccr. ... Kleiman FE, Manley JL (Sep 1999). "Functional interaction of BRCA1-associated BARD1 with polyadenylation factor CstF-50". ... Kleiman FE, Manley JL (Sep 1999). "Functional interaction of BRCA1-associated BARD1 with polyadenylation factor CstF-50". ... ADP-ribose) (PAR), which targets the BRCA1/BARD1 heterodimer to damaged DNA sites.[13] Double stranded breaks (DSB) in DNA ...
ADP ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily. ARF family ... The small ADP ribosylation factor (Arf) GTP-binding proteins are major regulators of vesicle biogenesis in intracellular ... Kahn RA, Kern FG, Clark J, Gelmann EP, Rulka C (1991). "Human ADP-ribosylation factors. A functionally conserved family of GTP- ... Amor JC, Harrison DH, Kahn RA, Ringe D (1994). "Structure of the human ADP-ribosylation factor 1 complexed with GDP". Nature. ...
ARL1 ADP ribosylation factor like GTPase 1 [Homo sapiens] ARL1 ADP ribosylation factor like GTPase 1 [Homo sapiens]. Gene ID: ... ADP-ribosylation factor-like protein 1. Names. ADP-ribosylation factor-like 1. ... ADP-ribosylation factor-like) family of proteins, which are structurally related to ADP-ribosylation factors (ARFs). ARFs, ... ADP ribosylation factor like GTPase 1provided by HGNC. Primary source. HGNC:HGNC:692 See related. Ensembl:ENSG00000120805 MIM: ...
Structure of human ADP-ribosylation factor 5. Atanassova, A., Tempel, W., Sundarajan, E., Dimov, S., Shehab, I., Lew, J., ... ADP-ribosylation factor 5. A. 192. Homo sapiens. Mutation(s): 0 Gene Names: ARF5. ...
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
ADP-ribosylation factor 4. Names. ADP-ribosylation factor 2. NP_001651.1. *EC 3.6.5.1 ... ARF4 ADP ribosylation factor 4 [Homo sapiens] ARF4 ADP ribosylation factor 4 [Homo sapiens]. Gene ID:378 ... ADP ribosylation factor 4provided by HGNC. Primary source. HGNC:HGNC:655 See related. Ensembl:ENSG00000168374 MIM:601177 Gene ... found ADP-ribosylation factor (ARF) 4 is one of the putative target genes of Mir-221, and the direct binding relationship was ...
ADP-ribosylation factor 6Add BLAST. 174. Amino acid modifications. Feature key. Position(s). DescriptionActions. Graphical view ... sp,P62332,ARF6_RAT ADP-ribosylation factor 6 OS=Rattus norvegicus GN=Arf6 PE=1 SV=2 ... Activation is generally mediated by guanine exchange factor (GEF), while inactivation through hydrolysis of bound GTP is ...
Compare ADP ribosylation factor interacting protein 1 ELISA Kits from leading suppliers on Biocompare. View specifications, ... ADP ribosylation factor interacting protein 1 ELISA Kits. The ELISA (enzyme-linked immunosorbent assay) is a widely used ... Bovine ADP-ribosylation factor-like protein 6-interacting protein 1 (ARL6IP1) ELISA Kit ... Canine ADP-ribosylation factor-like protein 6-interacting protein 1 (ARL6IP1) ELISA Kit ...
ADP Ribosylation Factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily. ARF family ... Retrieved from "https://www.wikidoc.org/index.php?title=ADP_ribosylation_factor&oldid=157557" ... GTPase activating proteins (GAPs) force ARF to hydrolyze bound GTP to GDP, and Guanine nucleotide exchange factors force ARF to ...
ADP-ribosylation factor-like protein 3. Details. Name. ADP-ribosylation factor-like protein 3. Kind. protein. Organism. Humans ... ADP-ribosylation factor-like protein 3. P36405. Details. Drug Relations. Drug Relations. DrugBank ID. Name. Drug group. ...
ADP-ribosylation factor guanine nucleotide-exchange factor 2 variantImported. ,p>Information which has been imported from ... tr,Q59FR3,Q59FR3_HUMAN ADP-ribosylation factor guanine nucleotide-exchange factor 2 variant (Fragment) OS=Homo sapiens OX=9606 ... ADP ribosylation factor guanine nucleotide exchange factor 2. PANTR. 1792. ADP ribosylation factor guanine nucleotide exchange ...
Molecular Aspects of the Cellular Activities of ADP-Ribosylation Factors. By Paul A. Randazzo, Zhongzhen Nie, Koichi Miura, ... Molecular Aspects of the Cellular Activities of ADP-Ribosylation Factors. By Paul A. Randazzo, Zhongzhen Nie, Koichi Miura, ... Molecular Aspects of the Cellular Activities of ADP-Ribosylation Factors Message Subject. (Your Name) has forwarded a page to ... Adenosine diphosphate-ribosylation factor (Arf) proteins are members of the Arf arm of the Ras superfamily of guanosine ...
Compare ADP ribosylation factor like GTPase 13A ELISA Kits from leading suppliers on Biocompare. View specifications, prices, ... ADP ribosylation factor like GTPase 13A ELISA Kits. The ELISA (enzyme-linked immunosorbent assay) is a well-established ... Your search returned 26 ADP ribosylation factor like GTPase 13A ELISA ELISA Kit across 2 suppliers. ... Background: Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a rational target... read more ...
ADP-RIBOSYLATION FACTOR, ADP-ribosylation factor 1, ADP-RIBOSYLATION FACTOR 1A, arf1, arf2, arf3, ATARF, ATARF1, ATARFA1A, ... ADP-ribosylation factor 1 , adp-ribosylation factor 1 , ADP-ribosylation factor domain protein 1 ... ADP-Ribosylation Factor 1 (ARF1) Antigen Profile Antigen Summary ADP-ribosylation factor 1 (ARF1) is a member of the human ARF ... Browse our anti-ADP-Ribosylation Factor 1 (ARF1) Antibodies. Full name:. anti-ADP-Ribosylation Factor 1 Antibodies (ARF1). On ...
ADP-ribosylation factor-like 2 (ARL2) has recently been implicated in OS formation through its association with Binder of ARL2 ... ADP ribosylation factor like 2 (Arl2) protein influences microtubule dynamics in breast cancer cells. Exp. Cell Res. 313, 473- ... ADP-ribosylation factor-like 2 (ARL2) has recently been implicated in OS formation through its association with Binder of ARL2 ... Specifically, the ADP-ribosylation factor-like 2 (ARL2) small GTPase was implicated in the process of OS formation in ...
ADP-ribosylation factor 4 , ADP-ribosylation factor 2 , ADP-ribosylation factor family-like protein ... ADP-Ribosylation Factor GTPase Activating Protein 3 ELISA Kits * ADP-Ribosylation Factor Guanine Nucleotide-Exchange Factor 1( ... Browse our ADP-Ribosylation Factor 4 (ARF4) ELISA Kits. Full name:. ADP-Ribosylation Factor 4 ELISA Kits (ARF4). On www. ... ADP-Ribosylation Factor GTPase Activating Protein 1 ELISA Kits * ADP-Ribosylation Factor GTPase Activating Protein 2 ELISA Kits ...
ADP-ribosylation factor 4 , ADP-ribosylation factor 2 , ADP-ribosylation factor family-like protein ... ADP-Ribosylation Factor GTPase Activating Protein 3 ELISA Kits * ADP-Ribosylation Factor Guanine Nucleotide-Exchange Factor 1( ... ADP-Ribosylation Factor 4 (ARF4) ELISA Kits. Bezeichnung:. ADP-Ribosylation Factor 4 ELISA Kits (ARF4). Auf www.antikoerper- ... ADP-Ribosylation Factor GTPase Activating Protein 1 ELISA Kits * ADP-Ribosylation Factor GTPase Activating Protein 2 ELISA Kits ...
Synonyms: ADP-ribosylation factor-like protein 13B, ADP-ribosylation factor-like protein 2-like 1, ARL2-like protein 1, ARL2L1 ...
ADP-ribosylation factor-like 4C LOC367311 similar to ADP-ribosylation factor-like protein 4C (ADP-ribosylation factor-like 7) ... ADP-ribosylation factor-like 4C; ADP-ribosylation factor-like 4C, pseudogene 1; ADP-ribosylation factor-like protein 4C; Arl4c- ... ARL4C (ADP ribosylation factor like GTPase 4C). RGD. RGD. Mus musculus (house mouse):. Arl4c (ADP-ribosylation factor-like 4C) ... ADP-ribosylation factor like GTPase 4C Name changed. 629549. APPROVED. 2015-11-19. Arl4c ADP-ribosylation factor like GTPase 4C ...
Synonyms: ADP-ribosylation factor-like protein 6-interacting protein 6, AIP-6, ARL-6-interacting protein 6, Aip-6, MGC33864 ...
ADP-ribosylation factor-like 9 explanation free. What is ADP-ribosylation factor-like 9? Meaning of ADP-ribosylation factor- ... Looking for online definition of ADP-ribosylation factor-like 9 in the Medical Dictionary? ... ADP-ribosylation factor-like 9 , definition of ADP-ribosylation factor-like 9 by Medical dictionary https://medical-dictionary. ... redirected from ADP-ribosylation factor-like 9) ARL9. A gene on chromosome 4q12 that encodes a GTPase which is similar to the ...
ADP-ribosylation factor-like 17 explanation free. What is ADP-ribosylation factor-like 17? Meaning of ADP-ribosylation factor- ... Looking for online definition of ADP-ribosylation factor-like 17 in the Medical Dictionary? ... ADP-ribosylation factor-like 17 , definition of ADP-ribosylation factor-like 17 by Medical dictionary https://medical- ... redirected from ADP-ribosylation factor-like 17) ARL17B. A gene on chromosome 17q21.31 that encodes a GTP-binding protein, ...
ARF, ADP-ribosylation factor, ARNO, ARF nucleotide-binding site opener, BFA, brefeldin A, COP, coat protein, GAP, GTPase- ... ADP-ribosylation factor GTPases in signal transduction and membrane traffic: independent functions? D. Jones D. Jones ... Potential regulation of ADP-ribosylation factor 6 signalling by phosphatidylinositol 3,4,5-trisphosphate Biochem Soc Trans ( ... ADP-ribosylation factor regulates spectrin skeleton assembly on the Golgi complex by stimulating phosphatidylinositol 4,5- ...
Vega: OTTMUSG6340 (Arf1, ADP-ribosylation factor 1)*CCDS: 24765, 24765.1*OMIM: ADP-RIBOSYLATION FACTOR 1; ARF1*Gene Ontology: ... ADP-ribosylation factor 1. Gene nomenclature, locus information, and GO, OMIM, and PMID associations are updated daily from MGI ...
ADP-ribosylation factor-like 5C. Synonyms: Arl12. Gene nomenclature, locus information, and GO, OMIM, and PMID associations are ... Vega: OTTMUSG2915 (Arl5c, ADP-ribosylation factor-like 5C)*CCDS: 25334, 25334.1*Gene Ontology: Arl5c *Mouse Phenome DB: Arl5c * ...
... stimulates cholera toxin ADP ribosylation. Mediates binding of non clathrin coated vesicles and AP1 (adaptor protein 1) of ... Arf6 - ADP ribosylation factor 6 (ARF6) is a member of the ADP ribosylation factor family of GTP binding proteins. ARF6 has a ... ADP ribosylation factor - Pfam box Symbol = Arf Name = width =250 caption =Membrane bound ADP ribosylation factor like protein ... ADP ribosylation factor 3, also known as ARF3, is a human gene.cite web , title = Entrez Gene: ARF3 ADP ribosylation factor 3, ...
ARF3A; ADP-ribosylation factor 3a; zgc:92190; wu:fc66h11; ADP-ribosylation factor 3 ...
Exosomal microRNA-122 mediates obesity-related cardiomyopathy through suppressing mitochondrial ADP-ribosylation factor-like 2 ... Mechanistically, miR-122 directly bound and suppressed mitochondrial protein ADP-ribosylation factor-like 2 (Arl-2) in vitro ... Exosomal microRNA-122 mediates obesity-related cardiomyopathy through suppressing mitochondrial ADP-ribosylation factor-like 2 ...
Top performende anti-Maus ADP-Ribosylation Factor 1 Antikörper für Immunofluorescence (Paraffin-embedded Sections) (IF (p)) ... Recommended ADP-Ribosylation Factor 1 Antibody (geliefert von: Anmelden zum Anzeigen ) Antigen ADP-Ribosylation Factor 1 (ARF1 ... anti-ADP-Ribosylation Factor 1 Antikörper * anti-Maus ADP-Ribosylation Factor 1 Antikörper für Immunofluorescence (Paraffin- ... Synonyms: ADP Ribosylation Factor 1, ADP-ribosylation factor 1, ARF 1, ARF1, ARF1_HUMAN, DJ133P16.1. ...
LdARL-3A, a Leishmania promastigote-specific ADP-ribosylation factor-like protein, is essential for flagellum integrity ... LdARL-3A, a Leishmania promastigote-specific ADP-ribosylation factor-like protein, is essential for flagellum integrity ... LdARL-3A, a Leishmania promastigote-specific ADP-ribosylation factor-like protein, is essential for flagellum integrity ... LdARL-3A, a Leishmania promastigote-specific ADP-ribosylation factor-like protein, is essential for flagellum integrity ...
ARF6 contains very low intrinsic GTP binding activity and depends on GEFs (guanine nucleotide exchange factors) such as CYTH3 ( ... ADP-ribosylation factor 6) small GTP binding protein plays critical roles in actin cytoskeleton rearrangements and membrane ... Guanine nucleotide exchange factors Is the Subject Area "Guanine nucleotide exchange factors" applicable to this article? Yes. ...
  • On www.antibodies-online.com are 194 ADP-Ribosylation Factor 1 (ARF1) Antibodies from 23 different suppliers available. (antibodies-online.com)
  • We report here that 2-methylcoprophilinamide (M- COPA (show COPA Antibodies )), a compound that induces disassembly of the Golgi apparatus by inactivating ADP-ribosylation factor 1 (Arf1 ), suppresses Stx (show ST8SIA2 Antibodies )-induced apoptosis. (antibodies-online.com)
  • ARF1 - ADP ribosylation factor 1, also known as ARF1, is a human gene. (academic.ru)
  • PBB Summary section title = summary text = ADP ribosylation factor 1 (ARF1) is a member of the human ARF gene family. (academic.ru)
  • Kinetics of Interaction between ADP-ribosylation Factor-1 (Arf1) and the Sec7 Domain of Arno Guanine Nucleotide Exchange Factor, Modulation by Allosteric Factors, and the Uncompetitive Inhibitor Brefeldin A. (inserm.fr)
  • The small GTP-binding protein ADP-ribosylation factor 1 (ARF1) is an essential component of the molecular machinery that catalyzes the formation of membranebound transport intermediates. (us.es)
  • The ADP-ribosylation factor 1 (Arf1) is required for maintenance of Golgi architecture and for vesicular trafficking, including the copper-responsive trafficking of ATP7A. (edu.au)
  • ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. (nih.gov)
  • Structure of an ADP-ribosylation factor, ARF1, from Entamoeba histolytica bound to Mg(2+)-GDP. (expasy.org)
  • Among the small GTP-binding proteins, ARF1 (ADP-ribosylation factor 1) and SAR1 (Secretion-Associated RAS super family 1) are commonly conserved among all eukaryotes with respect to both their functional and sequential characteristics. (mdpi.com)
  • ARF1 ‏ ( ADP ribosylation factor 1 ) هوَ بروتين يُشَفر بواسطة جين ARF1 في الإنسان . (wikipedia.org)
  • ADP ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily. (wikipedia.org)
  • The small ADP ribosylation factor (Arf) GTP-binding proteins are major regulators of vesicle biogenesis in intracellular traffic. (wikipedia.org)
  • GTPase activating proteins (GAPs) force ARF to hydrolyze bound GTP to GDP, and Guanine nucleotide exchange factors force ARF to adopt a new GTP molecule in place of a bound GDP. (wikipedia.org)
  • The protein encoded by this gene belongs to the ARL (ADP-ribosylation factor-like) family of proteins, which are structurally related to ADP-ribosylation factors (ARFs). (nih.gov)
  • This gene is a member of the human ARF gene family whose members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 5 ARF proteins and 11 ARF-like proteins and constitute one family of the RAS superfamily. (nih.gov)
  • Adenosine diphosphate-ribosylation factor (Arf) proteins are members of the Arf arm of the Ras superfamily of guanosine triphosphate (GTP)-binding proteins. (sciencemag.org)
  • The activities of some guanine nucleotide exchange factors (GEFs) and guanosine triphosphatase (GTPase)-activating proteins (GAPs) are stimulated by phosphoinositides, including phosphatidylinositol 3,4,5-trisphosphate (PIP3) and phosphatidylinositol 4,5-bisphosphate (PIP2), and phosphatidic acid (PA), likely providing both a means to respond to regulatory signals and a mechanism to coordinate GTP binding and hydrolysis. (sciencemag.org)
  • Additionally we are shipping ADP-Ribosylation Factor 1 Proteins (28) and many more products for this protein. (antibodies-online.com)
  • Additionally we are shipping ADP-Ribosylation Factor 4 Antibodies (70) and ADP-Ribosylation Factor 4 Proteins (18) and many more products for this protein. (antibodies-online.com)
  • ASAP1 is a scaffold bringing together the proteins needed for transport to the cilia including the GTP-binding protein Arf4 & the 2 G proteins of the Rab family-- Rab11 & Rab8 --linked by the Rab8 guanine nucleotide exchange factor Rabin8 . (antibodies-online.com)
  • Arf6 - ADP ribosylation factor 6 (ARF6) is a member of the ADP ribosylation factor family of GTP binding proteins. (academic.ru)
  • ADP-ribosylation factor (Arf) proteins are low molecular weight GTP binding proteins that belong to the Ras GTPase superfamily. (creativebiomart.net)
  • ADP-ribosylation factor 6, also known as ARF6, is a member of the ADP ribosylation factor family of GTP-binding proteins. (creativebiomart.net)
  • The ADP-ribosylation factor (ARF) genes are small GTP-binding proteins of the RAS superfamily. (biomol.com)
  • ADP-RIBOSYLATION FACTOR 1 is involved in regulating intracellular transport by modulating the interaction of coat proteins with organelle membranes in the early secretory pathway. (sickkids.ca)
  • Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). (enquirebio.com)
  • The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. (genecards.org)
  • The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. (nih.gov)
  • The trafficking of granule membrane proteins can be controlled by both luminal and cytosolic factors. (frontiersin.org)
  • Fundamental differences were previously discovered in the ADP-ribosylation of proteins from metaphase chromosomes and interphase nuclei of HeLa cells. (umn.edu)
  • Adolph, KW 1987, ' Factors influencing ADP-ribosylation differences between chromosomal proteins of interphase and metaphase HeLa cells ', Archives of Biochemistry and Biophysics , vol. 253, no. 1, pp. 176-188. (umn.edu)
  • Brefeldin A-inhibited guanine nucleotide-exchange proteins (GEPs) BIG1 and BIG2 activate ADP-ribosylation factor (ARF) GTPases, which are required for vesicular trafficking. (pnas.org)
  • ADP-ribosylation factors (ARFs) are 20-kDa guanine nucleotide-binding proteins with multiple intracellular functions, including critical roles in vesicular trafficking. (pnas.org)
  • Guanine-nucleotide exchange factors (GEFs), GDP dissociation inhibitors (GDIs), and GTPase-activating proteins are regulators of small GTP-binding proteins ( Figure 1 ). (mdpi.com)
  • BIG1 and BIG2: Brefeldin A-Inhibited Guanine Nucleotide-Exchange Proteins for ADP-Ribosylation Factors. (elsevier.com)
  • ADP-ribosylation factors (ARFs) are ∼20-kDa guanine nucleotide-binding proteins, which, like other members of the ras superfamily, are activated by exchanging bound GDP for GTP and inactivated through hydrolysis of the γ-phosphate of bound GTP to form GDP in a highly regulated cycle. (elsevier.com)
  • ADP-ribosylation factor (ARF) and ARF-like (ARL) proteins are small GTPases that undergo a GDP/GTP nucleotide exchange cycle. (regionh.dk)
  • ADP-ribosylation factors (ARF) are small G proteins that play key roles in vesicular transport processes. (biologists.com)
  • In addition to identifying most previously described PSD components, we also detected proteins involved in signaling to the nucleus as well as regulators of ADP-ribosylation factor signaling, ubiquitination, RNA trafficking, and protein translation. (mcponline.org)
  • There are 6 mammalian ARFs which are regulated by over 30 guanine nucleotide exchange factors (GEFs) and GTPase activating proteins (GAPs). (wikipedia.org)
  • The antibody recognizes the human human ADP ribosylation factor 6 (Arf6) protein. (medimabs.com)
  • ADP ribosylation factor 6 (Arf6) is a critical small GTPase that regulates membrane trafficking between the plasma membrane and endosomes. (elsevier.com)
  • ARF6 (ADP Ribosylation Factor 6) is a Protein Coding gene, and is affiliated with the lncRNA class. (genecards.org)
  • ADP ribosylation factor 6 (Arf6) is a small GTPase that regulates dendritic differentiation possibly through the organization of actin cytoskeleton and membrane traffic. (nih.gov)
  • Here, we characterized IQ-ArfGEF/BRAG1, a guanine nucleotide exchange factor (GEF) for Arf6, in the mouse brain. (nih.gov)
  • Your search returned 26 ADP ribosylation factor like GTPase 13A ELISA ELISA Kit across 2 suppliers. (biocompare.com)
  • Specifically, the ADP-ribosylation factor-like 2 (ARL2) small GTPase was implicated in the process of OS formation in photoreceptor cells through its interaction of the Retinitis Pigmentosa (RP)-linked gene, ARL2 Binding Protein (ARL2BP) 7 . (nature.com)
  • ADP ribosylation factor (ARF) is a GTPase involved in membrane traffic. (wikipedia.org)
  • Extragenic suppressors of hls1 were identified as mutations in AUXIN RESPONSE FACTOR 2 (ARF2). (nih.gov)
  • ARFs, described as activators of cholera toxin (CT) ADP-ribosyltransferase activity, regulate intracellular vesicular membrane trafficking, and stimulate a phospholipase D (PLD) isoform. (nih.gov)
  • Arfs are named for their activity as cofactors for cholera toxin-catalyzed adenosine diphosphate-ribosylation of the heterotrimeric G protein Gs. (sciencemag.org)
  • up-regulation of expression of microRNA-21-5p appears to increase IEC permeability and up-regulates ADP ribosylation factor 4 (Arf4/ARF4 ) in mouse and human cells. (antibodies-online.com)
  • Ubiquitous GTP-binding protein, approximately 20 kD, N-myristoylated, stimulates cholera toxin ADP-ribosylation. (academic.ru)
  • Because Gs and the A subunit of cholera toxin bind to ADP ribosylation factor (ARF) and could modulate its activity, we tested the sensitivity of antigen-evoked I CRAC to brefeldin A, an inhibitor of ARF-dependent functions, including vesicle transport. (rupress.org)
  • Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. (genecards.org)
  • A DNA sequence encoding the Homo sapiens (Human) ADP-ribosylation factor-like protein 2, was expressed in the hosts and tags indicated. (enquirebio.com)
  • On www.antibodies-online.com are 12 ADP-Ribosylation Factor 4 (ARF4) ELISA Kits from 1 different suppliers available. (antibodies-online.com)
  • Auf www.antikoerper-online.de finden Sie aktuell 12 ADP-Ribosylation Factor 4 (ARF4) ELISA Kits von 1 unterschiedlichen Herstellern. (antikoerper-online.de)
  • Cloning and characterization of the human ADP-ribosylation factor 4 gene. (nih.gov)
  • The authors investigated the relationship between PTEN status and the expression of a glioma-associated antigen, adenosine diphosphate-ribosylation factor 4-like (ARF4L) protein. (thejns.org)
  • found ADP-ribosylation factor (ARF) 4 is one of the putative target genes of Mir-221, and the direct binding relationship was further confirmed by dual-luciferase reporter assay. (nih.gov)
  • Distinct roles for the N- and C-terminal regions in the cytotoxicity of pierisin-1, a putative ADP-ribosylating toxin from cabbage butterfly, against mammalian cells. (biomedsearch.com)
  • Title: ARFRP1 functions upstream of ARL1 and ARL5 to coordinate recruitment of distinct tethering factors to the trans-Golgi network. (nih.gov)
  • The Golgi-localized, γ-ear containing, ADP-ribosylation factor binding (GGA) family of APs serve a similar role. (frontiersin.org)
  • ADP-ribosylation factor-like 2 (ARL2) has recently been implicated in OS formation through its association with Binder of ARL2 (BART or ARL2BP), a protein linked to inherited blinding disease. (nature.com)
  • Plays a role as an effector of the ADP-ribosylation factor-like protein 2, ARL2. (mybiosource.com)
  • Regulation of GRP1-catalyzed ADP ribosylation factor guanine nucleotid" by Jes K. Klarlund, Lucia E. Rameh et al. (umassmed.edu)
  • Moreover a global picture of alterations in signaling pathways in CRC was observed, including a significant up-regulation of different components of the epidermal growth factor receptor and Wnt/β-catenin pathways and the down-regulation of p14 ARF . (mcponline.org)
  • Tyrosine phosphorylation events play major roles in the initiation and regulation of several functional responses of human neutrophils stimulated by chemotactic factors such as the bacterially derived tripeptide formylmethionyl-leucyl-phenylalanine (fMet-Leu-Phe). (jimmunol.org)
  • LFM-A13 also negatively affected the translocation of Rac-2, RhoA, ADP ribosylation factor-1, Tec, Bmx, and Btk induced by fMet-Leu-Phe. (jimmunol.org)
  • Of particular relevance to the subject of the present investigation, stimulation of human neutrophils by chemotactic factors has very recently been observed to be associated with the translocation and activation of the PH domain-containing tyrosine kinases of the Tec family termed Tec, Btk and Bmx ( 16 ). (jimmunol.org)
  • Shin HW, Morinaga N, Noda M, Nakayama K. BIG2, a guanine nucleotide exchange factor for ADP-ribosylation factors: its localization to recycling endosomes and implication in the endosome integrity. (nih.gov)
  • The Arabidopsis BIG2 ARF-guanine nucleotide exchange factor (GEF) gene is expressed in several tissues and the encoded protein is a typical Arabidopsis ARF-GEF. (regionh.dk)
  • This reaction activates an ADP-ribosylation factor, a molecule that is involved in vesicle trafficking. (nih.gov)
  • Background: Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a rational target. (biocompare.com)
  • Epidermal growth factor receptor - This article is about a cell surface receptor. (academic.ru)
  • Is the Subject Area "Guanine nucleotide exchange factors" applicable to this article? (plos.org)
  • This receptor then binds to an ARF-GEF, a class of guanine nucleotide exchange factors. (wikipedia.org)
  • Activation is generally mediated by guanine exchange factor (GEF), while inactivation through hydrolysis of bound GTP is catalyzed by GTPases activating protein (GAP). (uniprot.org)
  • ADP-ribosylation factor GTPases in signal transduction and membrane traffic: independent functions? (portlandpress.com)
  • These studies demonstrate that both ethylene and light signals affect differential cell growth by acting through HLS1 to modulate the auxin response factors, pinpointing HLS1 as a key integrator of the signaling pathways that control hypocotyl bending. (nih.gov)
  • LOC101060021 (ADP-Ribosylation Factor 1 Pseudogene) is a Pseudogene. (genecards.org)
  • ADP-ribosylation factor-dependent phospholipase D2 activation is required for agonist-induced mu-opioid receptor endocytosis. (semanticscholar.org)
  • ADP-Ribosylation Factor 1" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (sickkids.ca)
  • 1.-Marshansky V - Identification of ADP-ribosylation factor-6 in brush-border membrane and early endosomes of human kidney proximal tubules. (medimabs.com)
  • Cellular levels of phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) are rapidly elevated in response to activation of growth factor receptor tyrosine kinases. (umassmed.edu)
  • Chemotactic Factor-Induced Recruitment and Activation of Tec Family Kinases in Human Neutrophils. (jimmunol.org)
  • A causative link between these two events is strongly suggested by the high affinity of the pleckstrin homology (PH) domains of Tec and Btk for PtdIns(3,4,5)P 3 ( 20 , 21 , 22 ) and by the wortmannin sensitivity of the recruitment of Tec kinases ( 16 ) and of the stimulation of the tyrosine phosphorylation cascade by chemotactic factors ( 23 ). (jimmunol.org)
  • Calcium influx is thought to be required for the secretion of inflammatory mediators, activation of transcription factors, and the elaboration of cytokines by rat mast cells stimulated through the high affinity receptor (FcεRI) for IgE. (rupress.org)
  • Cytohesin-2 phosphorylation by protein kinase C relieves the constitutive suppression of platelet dense granule secretion by ADP-ribosylation factor 6. (creativebiomart.net)
  • Brefeldin A blocked the enhancement of antigen-evoked I CRAC without inhibiting ADP ribosylation of Gsα, but it did not affect I CRAC induced by suboptimal antigen or by thapsigargin. (rupress.org)