ADP-Ribosylation Factors: MONOMERIC GTP-BINDING PROTEINS that were initially recognized as allosteric activators of the MONO(ADP-RIBOSE) TRANSFERASE of the CHOLERA TOXIN catalytic subunit. They are involved in vesicle trafficking and activation of PHOSPHOLIPASE D. This enzyme was formerly listed as EC 3.6.1.47ADP-Ribosylation Factor 1: ADP-RIBOSYLATION FACTOR 1 is involved in regulating intracellular transport by modulating the interaction of coat proteins with organelle membranes in the early secretory pathway. It is a component of COAT PROTEIN COMPLEX I. This enzyme was formerly listed as EC 3.6.1.47.Brefeldin A: A fungal metabolite which is a macrocyclic lactone exhibiting a wide range of antibiotic activity.Coated Vesicles: Vesicles formed when cell-membrane coated pits (COATED PITS, CELL-MEMBRANE) invaginate and pinch off. The outer surface of these vesicles are covered with a lattice-like network of coat proteins, such as CLATHRIN, coat protein complex proteins, or CAVEOLINS.Coatomer Protein: A 700-kDa cytosolic protein complex consisting of seven equimolar subunits (alpha, beta, beta', gamma, delta, epsilon and zeta). COATOMER PROTEIN and ADP-RIBOSYLATION FACTOR 1 are principle components of COAT PROTEIN COMPLEX I and are involved in vesicle transport between the ENDOPLASMIC RETICULUM and the GOLGI APPARATUS.Guanine Nucleotide Exchange Factors: Protein factors that promote the exchange of GTP for GDP bound to GTP-BINDING PROTEINS.GTP-Binding Proteins: Regulatory proteins that act as molecular switches. They control a wide range of biological processes including: receptor signaling, intracellular signal transduction pathways, and protein synthesis. Their activity is regulated by factors that control their ability to bind to and hydrolyze GTP to GDP. EC 3.6.1.-.Coat Protein Complex I: A protein complex comprised of COATOMER PROTEIN and ADP RIBOSYLATION FACTOR 1. It is involved in transport of vesicles between the ENDOPLASMIC RETICULUM and the GOLGI APPARATUS.Golgi Apparatus: A stack of flattened vesicles that functions in posttranslational processing and sorting of proteins, receiving them from the rough ENDOPLASMIC RETICULUM and directing them to secretory vesicles, LYSOSOMES, or the CELL MEMBRANE. The movement of proteins takes place by transfer vesicles that bud off from the rough endoplasmic reticulum or Golgi apparatus and fuse with the Golgi, lysosomes or cell membrane. (From Glick, Glossary of Biochemistry and Molecular Biology, 1990)GTPase-Activating Proteins: Proteins that activate the GTPase of specific GTP-BINDING PROTEINS.Adenosine Diphosphate Ribose: An ester formed between the aldehydic carbon of RIBOSE and the terminal phosphate of ADENOSINE DIPHOSPHATE. It is produced by the hydrolysis of nicotinamide-adenine dinucleotide (NAD) by a variety of enzymes, some of which transfer an ADP-ribosyl group to target proteins.Phospholipase D: An enzyme found mostly in plant tissue. It hydrolyzes glycerophosphatidates with the formation of a phosphatidic acid and a nitrogenous base such as choline. This enzyme also catalyzes transphosphatidylation reactions. EC 3.1.4.4.Guanosine 5'-O-(3-Thiotriphosphate): Guanosine 5'-(trihydrogen diphosphate), monoanhydride with phosphorothioic acid. A stable GTP analog which enjoys a variety of physiological actions such as stimulation of guanine nucleotide-binding proteins, phosphoinositide hydrolysis, cyclic AMP accumulation, and activation of specific proto-oncogenes.Guanosine Triphosphate: Guanosine 5'-(tetrahydrogen triphosphate). A guanine nucleotide containing three phosphate groups esterified to the sugar moiety.Nucleoside Diphosphate SugarsAdenosine Diphosphate Sugars: Esters formed between the aldehydic carbon of sugars and the terminal phosphate of adenosine diphosphate.Endocytosis: Cellular uptake of extracellular materials within membrane-limited vacuoles or microvesicles. ENDOSOMES play a central role in endocytosis.Protein Transport: The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Ribose: A pentose active in biological systems usually in its D-form.Membrane Proteins: Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.HeLa Cells: The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.ADP Ribose Transferases: Enzymes that transfer the ADP-RIBOSE group of NAD or NADP to proteins or other small molecules. Transfer of ADP-ribose to water (i.e., hydrolysis) is catalyzed by the NADASES. The mono(ADP-ribose)transferases transfer a single ADP-ribose. POLY(ADP-RIBOSE) POLYMERASES transfer multiple units of ADP-ribose to protein targets, building POLY ADENOSINE DIPHOSPHATE RIBOSE in linear or branched chains.Carrier Proteins: Transport proteins that carry specific substances in the blood or across cell membranes.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Cholera Toxin: An ENTEROTOXIN from VIBRIO CHOLERAE. It consists of two major protomers, the heavy (H) or A subunit and the B protomer which consists of 5 light (L) or B subunits. The catalytic A subunit is proteolytically cleaved into fragments A1 and A2. The A1 fragment is a MONO(ADP-RIBOSE) TRANSFERASE. The B protomer binds cholera toxin to intestinal epithelial cells, and facilitates the uptake of the A1 fragment. The A1 catalyzed transfer of ADP-RIBOSE to the alpha subunits of heterotrimeric G PROTEINS activates the production of CYCLIC AMP. Increased levels of cyclic AMP are thought to modulate release of fluid and electrolytes from intestinal crypt cells.Recombinant Fusion Proteins: Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Pertussis Toxin: One of the virulence factors produced by BORDETELLA PERTUSSIS. It is a multimeric protein composed of five subunits S1 - S5. S1 contains mono ADPribose transferase activity.Virulence Factors, Bordetella: A set of BACTERIAL ADHESINS and TOXINS, BIOLOGICAL produced by BORDETELLA organisms that determine the pathogenesis of BORDETELLA INFECTIONS, such as WHOOPING COUGH. They include filamentous hemagglutinin; FIMBRIAE PROTEINS; pertactin; PERTUSSIS TOXIN; ADENYLATE CYCLASE TOXIN; dermonecrotic toxin; tracheal cytotoxin; Bordetella LIPOPOLYSACCHARIDES; and tracheal colonization factor.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Adenosine Diphosphate: Adenosine 5'-(trihydrogen diphosphate). An adenine nucleotide containing two phosphate groups esterified to the sugar moiety at the 5'-position.Poly(ADP-ribose) Polymerases: Enzymes that catalyze the transfer of multiple ADP-RIBOSE groups from nicotinamide-adenine dinucleotide (NAD) onto protein targets, thus building up a linear or branched homopolymer of repeating ADP-ribose units i.e., POLY ADENOSINE DIPHOSPHATE RIBOSE.Cell Membrane: The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.Botulinum Toxins: Toxic proteins produced from the species CLOSTRIDIUM BOTULINUM. The toxins are synthesized as a single peptide chain which is processed into a mature protein consisting of a heavy chain and light chain joined via a disulfide bond. The botulinum toxin light chain is a zinc-dependent protease which is released from the heavy chain upon ENDOCYTOSIS into PRESYNAPTIC NERVE ENDINGS. Once inside the cell the botulinum toxin light chain cleaves specific SNARE proteins which are essential for secretion of ACETYLCHOLINE by SYNAPTIC VESICLES. This inhibition of acetylcholine release results in muscular PARALYSIS.NAD: A coenzyme composed of ribosylnicotinamide 5'-diphosphate coupled to adenosine 5'-phosphate by pyrophosphate linkage. It is found widely in nature and is involved in numerous enzymatic reactions in which it serves as an electron carrier by being alternately oxidized (NAD+) and reduced (NADH). (Dorland, 27th ed)Adenylate Cyclase: An enzyme of the lyase class that catalyzes the formation of CYCLIC AMP and pyrophosphate from ATP. EC 4.6.1.1.Adenylate Cyclase Toxin: One of the virulence factors produced by virulent BORDETELLA organisms. It is a bifunctional protein with both ADENYLYL CYCLASES and hemolysin components.Poly Adenosine Diphosphate Ribose: A polynucleotide formed from the ADP-RIBOSE moiety of nicotinamide-adenine dinucleotide (NAD) by POLY(ADP-RIBOSE) POLYMERASES.Phosphatidylinositol 4,5-Diphosphate: A phosphoinositide present in all eukaryotic cells, particularly in the plasma membrane. It is the major substrate for receptor-stimulated phosphoinositidase C, with the consequent formation of inositol 1,4,5-triphosphate and diacylglycerol, and probably also for receptor-stimulated inositol phospholipid 3-kinase. (Kendrew, The Encyclopedia of Molecular Biology, 1994)Bacterial Toxins: Toxic substances formed in or elaborated by bacteria; they are usually proteins with high molecular weight and antigenicity; some are used as antibiotics and some to skin test for the presence of or susceptibility to certain diseases.Kinetics: The rate dynamics in chemical or physical systems.Adaptor Proteins, Vesicular Transport: A class of proteins involved in the transport of molecules via TRANSPORT VESICLES. They perform functions such as binding to the cell membrane, capturing cargo molecules and promoting the assembly of CLATHRIN. The majority of adaptor proteins exist as multi-subunit complexes, however monomeric varieties have also been found.rhoA GTP-Binding Protein: A RHO GTP-BINDING PROTEIN involved in regulating signal transduction pathways that control assembly of focal adhesions and actin stress fibers. This enzyme was formerly listed as EC 3.6.1.47.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Azaguanine: One of the early purine analogs showing antineoplastic activity. It functions as an antimetabolite and is easily incorporated into ribonucleic acids.Intracellular Membranes: Thin structures that encapsulate subcellular structures or ORGANELLES in EUKARYOTIC CELLS. They include a variety of membranes associated with the CELL NUCLEUS; the MITOCHONDRIA; the GOLGI APPARATUS; the ENDOPLASMIC RETICULUM; LYSOSOMES; PLASTIDS; and VACUOLES.Enzyme Activation: Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.Biological Transport: The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Proteins: Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.NAD+ NucleosidaseCloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Tankyrases: A group of telomere associated proteins that interact with TRF1 PROTEIN, contain ANKYRIN REPEATS and have poly(ADP-ribose) polymerase activity.Cricetinae: A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.CHO Cells: CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). The species is a favorite for cytogenetic studies because of its small chromosome number. The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells.Mitochondrial ADP, ATP Translocases: A class of nucleotide translocases found abundantly in mitochondria that function as integral components of the inner mitochondrial membrane. They facilitate the exchange of ADP and ATP between the cytosol and the mitochondria, thereby linking the subcellular compartments of ATP production to those of ATP utilization.Signal Transduction: The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.

Identification of a new Pyk2 target protein with Arf-GAP activity. (1/1111)

Protein tyrosine kinase Pyk2 is activated by a variety of G-protein-coupled receptors and by extracellular signals that elevate intracellular Ca2+ concentration. We have identified a new Pyk2 binding protein designated Pap. Pap is a multidomain protein composed of an N-terminal alpha-helical region with a coiled-coil motif, followed by a pleckstrin homology domain, an Arf-GAP domain, an ankyrin homology region, a proline-rich region, and a C-terminal SH3 domain. We demonstrate that Pap forms a stable complex with Pyk2 and that activation of Pyk2 leads to tyrosine phosphorylation of Pap in living cells. Immunofluorescence experiments demonstrate that Pap is localized in the Golgi apparatus and at the plasma membrane, where it is colocalized with Pyk2. In addition, in vitro recombinant Pap exhibits strong GTPase-activating protein (GAP) activity towards the small GTPases Arf1 and Arf5 and weak activity towards Arf6. Addition of recombinant Pap protein to Golgi preparations prevented Arf-dependent generation of post-Golgi vesicles in vitro. Moreover, overexpression of Pap in cultured cells reduced the constitutive secretion of a marker protein. We propose that Pap functions as a GAP for Arf and that Pyk2 may be involved in regulation of vesicular transport through its interaction with Pap.  (+info)

ARF6 requirement for Rac ruffling suggests a role for membrane trafficking in cortical actin rearrangements. (2/1111)

The ARF6 GTPase regulates a novel endosomal-plasma membrane recycling pathway and influences cortical actin remodeling. Here we examined the relationship between ARF6 and Rac1, a Rho family GTPase, implicated in cortical actin rearrangements. Endogenous Rac1 colocalized with ARF6 at the plasma membrane and on the ARF6 recycling endosome in untransfected HeLa and primary human fibroblast cells. In transfected HeLa cells Rac1 and ARF6 also colocalized. Cells expressing wild-type ARF6 or Rac1 formed actin-containing surface protrusions and membrane ruffles, respectively, upon treatment with the G protein activator aluminum fluoride. Aluminum fluoride-treatment of cells transfected with equivalent amounts of plasmid resulted in enhanced membrane ruffling, with protrusions appearing as Rac expression was lowered. Co-expression of the dominant negative, GTP binding-defective ARF6 T27N mutant inhibited the aluminum fluoride-induced ruffling observed in cells expressing Rac1, and the constitutive ruffling observed in cells expressing the activated Rac1 Q61L mutant. In contrast, co-expression of the GTP-binding-defective, T17N mutant of either Rac1 or Cdc42 with ARF6 did not inhibit the aluminum fluoride-induced surface protrusions, nor did inactivation of Rho with C3-transferase. These observations suggest that ARF6, a non-Rho family GTPase, can, by itself, alter cortical actin and can influence the ability of Rac1 to form lamellipodia, in part, by regulating its trafficking to the plasma membrane.  (+info)

Coupling of coat assembly and vesicle budding to packaging of putative cargo receptors. (3/1111)

COPI-coated vesicle budding from lipid bilayers whose composition resembles mammalian Golgi membranes requires coatomer, ARF, GTP, and cytoplasmic tails of putative cargo receptors (p24 family proteins) or membrane cargo proteins (containing the KKXX retrieval signal) emanating from the bilayer surface. Liposome-derived COPI-coated vesicles are similar to their native counterparts with respect to diameter, buoyant density, morphology, and the requirement for an elevated temperature for budding. These results suggest that a bivalent interaction of coatomer with membrane-bound ARF[GTP] and with the cytoplasmic tails of cargo or putative cargo receptors is the molecular basis of COPI coat assembly and provide a simple mechanism to couple uptake of cargo to transport vesicle formation.  (+info)

GCS1, an Arf guanosine triphosphatase-activating protein in Saccharomyces cerevisiae, is required for normal actin cytoskeletal organization in vivo and stimulates actin polymerization in vitro. (4/1111)

Recent cloning of a rat brain phosphatidylinositol 3,4, 5-trisphosphate binding protein, centaurin alpha, identified a novel gene family based on homology to an amino-terminal zinc-binding domain. In Saccharomyces cerevisiae, the protein with the highest homology to centaurin alpha is Gcs1p, the product of the GCS1 gene. GCS1 was originally identified as a gene conditionally required for the reentry of cells into the cell cycle after stationary phase growth. Gcs1p was previously characterized as a guanosine triphosphatase-activating protein for the small guanosine triphosphatase Arf1, and gcs1 mutants displayed vesicle-trafficking defects. Here, we have shown that similar to centaurin alpha, recombinant Gcs1p bound phosphoinositide-based affinity resins with high affinity and specificity. A novel GCS1 disruption strain (gcs1Delta) exhibited morphological defects, as well as mislocalization of cortical actin patches. gcs1Delta was hypersensitive to the actin monomer-sequestering drug, latrunculin-B. Synthetic lethality was observed between null alleles of GCS1 and SLA2, the gene encoding a protein involved in stabilization of the actin cytoskeleton. In addition, synthetic growth defects were observed between null alleles of GCS1 and SAC6, the gene encoding the yeast fimbrin homologue. Recombinant Gcs1p bound to actin filaments, stimulated actin polymerization, and inhibited actin depolymerization in vitro. These data provide in vivo and in vitro evidence that Gcs1p interacts directly with the actin cytoskeleton in S. cerevisiae.  (+info)

EFA6, a sec7 domain-containing exchange factor for ARF6, coordinates membrane recycling and actin cytoskeleton organization. (5/1111)

We have identified a human cDNA encoding a novel protein, exchange factor for ARF6 (EFA6), which contains Sec7 and pleckstrin homology domains. EFA6 promotes efficient guanine nucleotide exchange on ARF6 and is distinct from the ARNO family of ARF1 exchange factors. The protein localizes to a dense matrix on the cytoplasmic face of plasma membrane invaginations, induced on its expression. We show that EFA6 regulates endosomal membrane recycling and promotes the redistribution of transferrin receptors to the cell surface. Furthermore, expression of EFA6 induces actin-based membrane ruffles that are inhibited by co-expression of dominant-inhibitory mutant forms of ARF6 or Rac1. Our results demonstrate that by catalyzing nucleotide exchange on ARF6 at the plasma membrane and by regulating Rac1 activation, EFA6 coordinates endocytosis with cytoskeletal rearrangements.  (+info)

Structural basis for the inhibitory effect of brefeldin A on guanine nucleotide-exchange proteins for ADP-ribosylation factors. (6/1111)

Protein secretion through the endoplasmic reticulum and Golgi vesicular trafficking system is initiated by the binding of ADP-ribosylation factors (ARFs) to donor membranes, leading to recruitment of coatomer, bud formation, and eventual vesicle release. ARFs are approximately 20-kDa GTPases that are active with bound GTP and inactive with GDP bound. Conversion of ARF-GDP to ARF-GTP is regulated by guanine nucleotide-exchange proteins. All known ARF guanine nucleotide-exchange proteins contain a Sec7 domain of approximately 200 amino acids that includes the active site and fall into two classes that differ in molecular size and susceptibility to inhibition by the fungal metabolite brefeldin A (BFA). To determine the structural basis of BFA sensitivity, chimeric molecules were constructed by using sequences from the Sec7 domains of BFA-sensitive yeast Sec7 protein (ySec7d) and the insensitive human cytohesin-1 (C-1Sec7). Based on BFA inhibition of the activities of these molecules with recombinant yeast ARF2 as substrate, the Asp965-Met975 sequence in ySec7d was shown to be responsible for BFA sensitivity. A C-1Sec7 mutant in which Ser199, Asn204, and Pro209 were replaced with the corresponding ySec7d amino acids, Asp965, Gln970, and Met975, exhibited BFA sensitivity similar to that of recombinant ySec7d (rySec7d). Single replacement in C-1Sec7 of Ser199 or Pro209 resulted in partial inhibition by BFA, whereas replacement of Gln970 in ySec7d with Asn (as found in C-1Sec7) had no effect. As predicted, the double C-1Sec7 mutant with S199D and P209M was BFA-sensitive, demonstrating that Asp965 and Met975 in ySec7d are major molecular determinants of BFA sensitivity.  (+info)

Characterization of the regulation of phospholipase D activity in the detergent-insoluble fraction of HL60 cells by protein kinase C and small G-proteins. (7/1111)

Phospholipase D (PLD) activity has been shown to be GTP-dependent both in vivo and in vitro. One protein that confers GTP sensitivity to PLD activity in vitro is the low-molecular-mass G-protein ADP-ribosylation factor (Arf). However, members of the Rho family and protein kinase C (PKC) have also been reported to activate PLD in various cell systems. We have characterized the stimulation of PLD in HL60 cell membranes by these proteins. The results demonstrate that a considerable proportion of HL60 PLD activity is located in a detergent-insoluble fraction of the cell membrane that is unlikely to be a caveolae-like domain, but is probably cytoskeletal. This PLD activity required the presence of Arf1, a Rho-family member and PKC for efficient catalysis of the lipid substrate, suggesting that the activity represents PLD1. We show that recombinant human PLD1b is regulated in a similar manner to HL60-membrane PLD, and that PKCalpha and PKCdelta are equally effective PLD activators. Therefore maximum PLD activity requires Arf, a Rho-family member and PKC, emphasizing the high degree of regulation of this enzyme.  (+info)

The ADP-ribosylation factor (ARF)-related GTPase ARF-related protein binds to the ARF-specific guanine nucleotide exchange factor cytohesin and inhibits the ARF-dependent activation of phospholipase D. (8/1111)

ADP-ribosylation factor-related protein (ARP) is a membrane-associated GTPase with remote similarity to the family of ADP-ribosylation factors (ARF). In a yeast two-hybrid screen designed to identify proteins interacting with ARP, we isolated a partial cDNA of the ARF-specific guanine nucleotide exchange factor mSec7-1/cytohesin encoding its N terminus and most of the Sec7 domain (codons 1-200). ARP and ARP-Q79L (GTPase-negative ARP) exhibited a higher affinity to mSec7-1-(1-200) than ARP-T31N (nucleotide exchange-defective ARP) in the two-hybrid assay. Similarly, full-length [35S]mSec7-1/cytohesin was specifically adsorbed to glutathione-Sepharose loaded with glutathione S-transferase (GST)-ARP-Q79L, GST-ARP, or GST-ARP-T31N, the latter exhibiting the lowest binding affinity. Overexpression of ARP-Q79L, but not of ARP-T31N, in COS-7 cells reduced the fluorescence from co-expressed green fluorescent protein fused with mSec7-1/cytohesin or mSec7-2/ARNO in plasma membranes as detected by deconvolution microscopy. Recombinant ARP and ARP-Q79L, but not ARP-T31N, inhibited the phospholipase D (PLD) activity stimulated by mSec7-2/ARNO and ARF in a system of isolated membranes. Furthermore, transfection of HEK-293 cells with ARP or ARP-Q79L, but not ARP-T31N, inhibited the muscarinic acetylcholine receptor-3 induced PLD stimulation and translocation of ARF from cytosol to membranes. These data suggest that the GTP-bound form of ARP specifically binds mSec7-1/cytohesin, and that ARP may be involved in a pathway inhibiting the ARF-controlled activity of PLD.  (+info)

*GPLD1

2005). "Exocytosis of CTLA-4 is dependent on phospholipase D and ADP ribosylation factor-1 and stimulated during activation of ... 2005). "Characterization of primate trypanosome lytic factors". Mol. Biochem. Parasitol. 138 (1): 9-20. doi:10.1016/j. ...

*ADP ribosylation factor

ADP ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily. ARF family ... The small ADP ribosylation factor (Arf) GTP-binding proteins are major regulators of vesicle biogenesis in intracellular ... Kahn RA, Kern FG, Clark J, Gelmann EP, Rulka C (1991). "Human ADP-ribosylation factors. A functionally conserved family of GTP- ... Amor JC, Harrison DH, Kahn RA, Ringe D (1994). "Structure of the human ADP-ribosylation factor 1 complexed with GDP". Nature. ...

*ARF1

ADP-ribosylation factor 1 is a protein that in humans is encoded by the ARF1 gene. ADP-ribosylation factor 1 (ARF1) is a member ... Frank S, Upender S, Hansen SH, Casanova JE (1998). "ARNO is a guanine nucleotide exchange factor for ADP-ribosylation factor 6 ... ARF1 ADP-ribosylation factor 1". Mitchell R, Robertson DN, Holland PJ, Collins D, Lutz EM, Johnson MS (September 2003). "ADP- ... 1991). "ADP-ribosylation factor is a subunit of the coat of Golgi-derived COP-coated vesicles: a novel role for a GTP-binding ...

*Phospholipase D1

Activation of the purified enzymes by phosphatidylinositol 4,5-bisphosphate, ADP-ribosylation factor, and Rho family monomeric ... ADP-ribosylation factor regulates hPLD2". J. Biol. Chem. 273 (21): 12846-52. doi:10.1074/jbc.273.21.12846. PMID 9582313. Kim JH ... "Human ADP-ribosylation factor-activated phosphatidylcholine-specific phospholipase D defines a new and highly conserved gene ... "Activation of phospholipase D1 by direct interaction with ADP-ribosylation factor 1 and RalA". FEBS Lett. 430 (3): 231-5. doi: ...

*PLD2

ADP-ribosylation factor regulates hPLD2". J Biol Chem. 273 (21): 12846-52. doi:10.1074/jbc.273.21.12846. PMID 9582313. "Entrez ... "Cardiac phospholipase D2 localizes to sarcolemmal membranes and is inhibited by alpha-actinin in an ADP-ribosylation factor- ... "Cardiac phospholipase D2 localizes to sarcolemmal membranes and is inhibited by alpha-actinin in an ADP-ribosylation factor- ... "The direct interaction of phospholipase C-gamma 1 with phospholipase D2 is important for epidermal growth factor signaling". J ...

*ARL13B

ADP-ribosylation factor-like protein 13B (ARL13B), also known as ADP-ribosylation factor-like protein 2-like 1, is a protein ... This gene encodes a member of the ADP-ribosylation factor-like family. The encoded protein is a small GTPase that contains both ... ADP-ribosylation factor-like 13B". Cantagrel V, Silhavy JL, Bielas SL, Swistun D, Marsh SE, Bertrand JY, Audollent S, Attié- ...

*ARL8A

ADP-ribosylation factor-like protein 8A is a protein that in humans is encoded by the ARL8A gene. GRCh38: Ensembl release 89: ... "Entrez Gene: ARL8A ADP-ribosylation factor-like 8A". Human ARL8A genome location and ARL8A gene details page in the UCSC Genome ...

*ARL6

ADP-ribosylation factor-like protein 6 is a protein that in humans is encoded by the ARL6 gene. The protein encoded by this ... "Entrez Gene: ARL6 ADP-ribosylation factor-like 6". GeneReviews/NIH/NCBI/UW entry on Bardet-Biedl Syndrome Human ARL6 genome ... 1999). "ADP-ribosylation factor (ARF)-like 4, 6, and 7 represent a subgroup of the ARF family characterization by rapid ... 1999). "A novel ADP-ribosylation like factor (ARL-6), interacts with the protein-conducting channel SEC61beta subunit". FEBS ...

*ARL2

ADP-ribosylation factor-like protein 2 is a protein that in humans is encoded by the ARL2 gene. The ADP-ribosylation factor ( ... "Entrez Gene: ARL2 ADP-ribosylation factor-like 2". Kobayashi A, Kubota S, Mori N, McLaren MJ, Inana G (January 2003). " ... Beghin A, Honore S, Messana C, Matera EL, Aim J, Burlinchon S, Braguer D, Dumontet C (2007). "ADP ribosylation factor like 2 ( ... Bhamidipati A, Lewis SA, Cowan NJ (May 2000). "ADP ribosylation factor-like protein 2 (Arl2) regulates the interaction of ...

*ARF6

ADP-ribosylation factor 6 (ARF6) is a member of the ADP ribosylation factor family of GTP-binding proteins. ARF6 has a variety ... Frank S, Upender S, Hansen SH, Casanova JE (1998). "ARNO is a guanine nucleotide exchange factor for ADP-ribosylation factor 6 ... "ADP-ribosylation factor 6 as a target of guanine nucleotide exchange factor GRP1". J. Biol. Chem. 274 (38): 27099-104. doi: ... Amor JC, Harrison DH, Kahn RA, Ringe D (1995). "Structure of the human ADP-ribosylation factor 1 complexed with GDP". Nature. ...

*ARF4

ADP-ribosylation factor 4 is a protein that in humans is encoded by the ARF4 gene. ADP-ribosylation factor 4 (ARF4) is a member ... ARF4 ADP-ribosylation factor 4". Kim SW, Hayashi M, Lo JF, Yang Y, Yoo JS, Lee JD (Jan 2003). "ADP-ribosylation factor 4 small ... "ADP-ribosylation factor 4 small GTPase mediates epidermal growth factor receptor-dependent phospholipase D2 activation". J. ... Kahn RA, Kern FG, Clark J, Gelmann EP, Rulka C (1991). "Human ADP-ribosylation factors. A functionally conserved family of GTP- ...

*ARL15

ADP-ribosylation factor-like 15 is a protein in humans that is encoded by the ARL15 gene. GRCh38: Ensembl release 89: ... ADP-ribosylation factor-like 15". Retrieved 2013-03-16. ...

*ARF3

ADP-ribosylation factor 3 is a protein that in humans is encoded by the ARF3 gene. ADP-ribosylation factor 3 (ARF3) is a member ... "Entrez Gene: ARF3 ADP-ribosylation factor 3". Kanoh H, Williger BT, Exton JH (February 1997). "Arfaptin 1, a putative cytosolic ... Williger BT, Provost JJ, Ho WT, Milstine J, Exton JH (July 1999). "Arfaptin 1 forms a complex with ADP-ribosylation factor and ... Lee CM, Haun RS, Tsai SC, Moss J, Vaughan M (1992). "Characterization of the human gene encoding ADP-ribosylation factor 1, a ...

*ARL4A

ADP-ribosylation factor-like protein 4A is a protein that in humans is encoded by the ARL4A gene. ADP-ribosylation factor-like ... Jacobs S, Schilf C, Fliegert F, Koling S, Weber Y, Schürmann A, Joost HG (1999). "ADP-ribosylation factor (ARF)-like 4, 6, and ... ARL4A ADP-ribosylation factor-like 4A". Lin CY, Huang PH, Liao WL, Cheng HJ, Huang CF, Kuo JC, Patton WA, Massenburg D, Moss J ... 4A is a member of the ADP-ribosylation factor family of GTP-binding proteins. ARL4A is similar to ARL4C and ARL4D and each has ...

*ARL4D

ADP-ribosylation factor-like protein 4D is a protein that in humans is encoded by the ARL4D gene. ADP-ribosylation factor 4D is ... "Entrez Gene: ARL4D ADP-ribosylation factor-like 4D". "DEXA data for Arl4d". Wellcome Trust Sanger Institute. "Radiography data ... Jacobs S, Schilf C, Fliegert F, Koling S, Weber Y, Schürmann A, Joost HG (Aug 1999). "ADP-ribosylation factor (ARF)-like 4, 6, ... "Recognition of ADP-ribosylation factor 4-like by HLA-A2-restricted and tumor-reactive cytotoxic T lymphocytes from patients ...

*ARL1

ADP-ribosylation factor-like) family of proteins, which are structurally related to ADP-ribosylation factors (ARFs). ARFs, ... ADP-ribosylation factor-like protein 1 is a protein that in humans is encoded by the ARL1 gene. The protein encoded by this ... Van Valkenburgh H, Shern JF, Sharer JD, Zhu X, Kahn RA (June 2001). "ADP-ribosylation factors (ARFs) and ARF-like 1 (ARL1) have ... Van Valkenburgh H, Shern JF, Sharer JD, Zhu X, Kahn RA (2001). "ADP-ribosylation factors (ARFs) and ARF-like 1 (ARL1) have both ...

*ARL13A

ADP ribosylation factor like GTPase 13A is a protein that in humans is encoded by the ARL13A gene. GRCh38: Ensembl release 89: ... ADP ribosylation factor like GTPase 13A". Retrieved 2017-02-09. ...

*Golgi apparatus

BFA blocks the activation of some ADP-ribosylation factors (ARFs). ARFs are small GTPases which regulate vesicular trafficking ... BFA inhibits the function of several guanine nucleotide exchange factors (GEFs) that mediate GTP-binding of ARFs. Treatment of ...

*Beta adrenergic receptor kinase

Premont RT, Claing A, Vitale N, Perry SJ, Lefkowitz RJ (July 2000). "The GIT family of ADP-ribosylation factor GTPase- ... a G protein-coupled receptor kinase-associated ADP ribosylation factor GTPase-activating protein". Proc. Natl. Acad. Sci. U.S.A ... receptor kinase 2 in a signaling unit that regulates p42/p44 mitogen-activated protein kinase by epidermal growth factor". J. ...

*GIT1

Premont RT, Claing A, Vitale N, Perry SJ, Lefkowitz RJ (August 2000). "The GIT family of ADP-ribosylation factor GTPase- ... a G protein-coupled receptor kinase-associated ADP ribosylation factor GTPase-activating protein". Proc Natl Acad Sci U S A. 95 ... Zhao ZS, Manser E, Loo TH, Lim L (September 2000). "Coupling of PAK-interacting exchange factor PIX to GIT1 promotes focal ... Zhao ZS, Manser E, Loo TH, Lim L (2000). "Coupling of PAK-interacting exchange factor PIX to GIT1 promotes focal complex ...

*ARFRP1

ADP-ribosylation factor-related protein 1 is a protein that in humans is encoded by the ARFRP1 gene. The protein encoded by ... "Entrez Gene: ARFRP1 ADP-ribosylation factor related protein 1". Schürmann A, Schmidt M, Asmus M, Bayer S, Fliegert F, Koling S ... It is related to the ADP-ribosylation factor (ARF) and ARF-like (ARL) genes. The gene is located in a gene cluster that ... 1999). "The ADP-ribosylation factor (ARF)-related GTPase ARF-related protein binds to the ARF-specific guanine nucleotide ...

*ARL6IP1

ADP-ribosylation factor-like protein 6-interacting protein 1 is a protein that in humans is encoded by the ARL6IP1 gene. GRCh38 ... ARL6IP1 ADP-ribosylation factor-like 6 interacting protein 1". Human ARL6IP1 genome location and ARL6IP1 gene details page in ... "A novel ADP-ribosylation like factor (ARL-6), interacts with the protein-conducting channel SEC61beta subunit". FEBS Lett. 459 ... ADP-ribosylation-like factor-6 interacting protein (ARL6)". Genomics. 68 (3): 351-4. doi:10.1006/geno.2000.6278. PMID 10995579 ...

*ARFIP1

"Entrez Gene: ARFIP1 ADP-ribosylation factor interacting protein 1 (arfaptin 1)". Tsai SC, Adamik R, Hong JX, et al. (1998). " ... Williger BT, Provost JJ, Ho WT, Milstine J, Exton JH (Aug 1999). "Arfaptin 1 forms a complex with ADP-ribosylation factor and ... Ho WT, Exton JH, Williger BT (2003). "Arfaptin 1 inhibits ADP-ribosylation factor-dependent matrix metalloproteinase-9 ... a putative cytosolic target protein of ADP-ribosylation factor, is recruited to Golgi membranes". J Biol Chem. 272 (9): 5421-9 ...

*GIT2

Premont RT, Claing A, Vitale N, Perry SJ, Lefkowitz RJ (Jul 2000). "The GIT family of ADP-ribosylation factor GTPase-activating ... GIT proteins interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating ... "An ADP-ribosylation factor GTPase-activating protein Git2-short/KIAA0148 is involved in subcellular localization of paxillin ... a G protein-coupled receptor kinase-associated ADP ribosylation factor GTPase-activating protein". Proceedings of the National ...

*ARL3

ADP-ribosylation factor-like protein 3 is a protein that in humans is encoded by the ARL3 gene. ADP-ribosylation factor-like 3 ... Van Valkenburgh H, Shern JF, Sharer JD, Zhu X, Kahn RA (June 2001). "ADP-ribosylation factors (ARFs) and ARF-like 1 (ARL1) have ... Van Valkenburgh H, Shern JF, Sharer JD, Zhu X, Kahn RA (2001). "ADP-ribosylation factors (ARFs) and ARF-like 1 (ARL1) have both ... Kim HS (March 1999). "Assignment of the human ADP-ribosylation factor-like 3 (ARL3) gene to chromosome 10 band q23.3 by ...

*CENTG2

... belongs to an ADP-ribosylation factor GTPase-activating (ARF-GAP) protein family involved in membrane traffic and actin ... phosphoinositide-dependent ADP-ribosylation factor GTPase-activating protein that affects actin cytoskeleton". The Journal of ...

*ARFGAP3

ADP-ribosylation factor GTPase-activating protein 3 is a protein that in humans is encoded by the ARFGAP3 gene. The protein ... "Entrez Gene: ARFGAP3 ADP-ribosylation factor GTPase activating protein 3". Human ARFGAP1 genome location and ARFGAP1 gene ... which associates with the Golgi apparatus and which is thought to interact with ADP-ribosylation factor 1 (ARF1). The encoded ...
Looking for online definition of ADP-ribosylation factor-like tumour-suppressor protein 1 in the Medical Dictionary? ADP-ribosylation factor-like tumour-suppressor protein 1 explanation free. What is ADP-ribosylation factor-like tumour-suppressor protein 1? Meaning of ADP-ribosylation factor-like tumour-suppressor protein 1 medical term. What does ADP-ribosylation factor-like tumour-suppressor protein 1 mean?
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Myc-DDK-tagged ORF clone of Homo sapiens ADP-ribosylation factor-like 5C (ARL5C) as transfection-ready DNA - 10 µg - OriGene - cdna clones
Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. Regulates formation of new microtubules and centrosome integrity. Prevents the TBCD-induced microtubule destruction. Participates in association with TBCD, in the disassembly of the apical junction complexes. Antagonizes the effect of TBCD on epithelial cell detachment and tight and adherens junctions disassembly. Together with ARL2, plays a role in the nuclear translocation, retention and transcriptional activity of STAT3. Component of a regulated secretory pathway involved in Ca(2+)-dependent release of acetylcholine. Required for normal progress through the cell cycle.
Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). Required for normal cytokinesis and cilia signaling. Requires assistance from GTPase-activating proteins (GAPs) like RP2 and PDE6D, in order to cycle between inactive GDP-bound and active GTP-bound forms. Required for targeting proteins such as NPHP3 to the ciliary membrane by releasing myristoylated NPHP3 from UNC119B cargo adapter into the cilium. Does not act as an allosteric activator of the cholera toxin catalytic subunit ...
Guanine nucleotide exchange factor for Arf GTPases, stimulating the nucleotide exchange from the GDP-bound to the GTP-bound form. Catalyzes both the GDP release by and the GTP binding to ARF2. Has no exhange activity on Rab GTPases. Involved in vesicular transport.
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The KOMP Repository is located at the University of California Davis and Childrens Hospital Oakland Research Institute. Question? Comments? For Mice, Cells, and germplasm please contact us at [email protected], US 1-888-KOMP-MICE or International +1-530-752-KOMP, or for vectors [email protected] or +1-510-450-7917 ...
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Ver más] The small GTP-binding protein ADP-ribosylation factor 1 (ARF1) is an essential component of the molecular machinery that catalyzes the formation of membranebound transport intermediates. By using an in vitro assay that reproduces recruitment of cytosolic proteins onto purified, high salt-washed Golgi membranes, we have analyzed the role of cAMP-dependent protein kinase A (PKA) on ARF1 incorporation. Addition to this assay of either pure catalytic subunits of PKA (C-PKA) or cAMP increased ARF1 binding. By contrast, ARF1 association was inhibited following C-PKA inactivation with either PKA inhibitory peptide or RIIa as well as after cytosol depletion of C-PKA. C-PKA also stimulated recruitment and activation of a recombinant form of human ARF1 in the absence of additional cytosolic components. The binding step could be dissociated from the activation reaction and found to be independent of guanine nucleotides and saturable. This step was stimulated by C-PKA in an ATP-dependent manner. ...
ADP-ribosylation factor-like protein 4A (ARL4A) is a developmentally regulated member of the ARF/ARL GTPase family. The primary structure of ARL4A is very similar to that of other ARF/ARL molecules, but its function remains unclear. The trans-Golgi network golgin GCC185 is required for maintenance of Golgi structure and distinct endosome-to-Golgi transport. We show here that GCC185 acts as a new effector for ARL4 to modulate Golgi organization. ARL4A directly interacts with GCC185 in a GTP-dependent manner. Sub-coiled-coil regions of the CC2 domain of GCC185 are required for the interaction between GCC185 and ARL4A. Depletion of ARL4A reproduces the GCC185-depleted phenotype, causing fragmentation of the Golgi compartment and defects in endosome-to-Golgi transport. GCC185 and ARL4A localize to the Golgi independently of each other. Deletion of the ARL4A-interacting region of GCC185 results in inability to maintain Golgi structure. Depletion of ARL4A impairs the interaction between GCC185 and ...
This gene encodes a member of an ADP-ribosylation factor GTPase-activating protein family involved in membrane trafficking and cytoskeleton dynamics. This gene functions as a direct regulator of the adaptor-related protein complex 3 on endosomes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011 ...
ARL2 - ARL2 (untagged)-Human ADP-ribosylation factor-like 2 (ARL2), transcript variant 1 available for purchase from OriGene - Your Gene Company.
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We have demonstrated previously the potent activation of PLD by the chemokine IL-8 in T lymphocytes (18). We have now extended our findings to include the C-C chemokine RANTES in demonstrating that in the Jurkat T cell line, the activation of this enzyme occurs at subnanomolar concentrations and is dependent on the activation of small GTP-binding protein cofactors. RANTES-induced PLD activation is consistently maximal at 1 nM, a concentration corresponding to the optimal chemotaxis-inducing dose in normal T lymphocytes. Interestingly, PLD activation in T lymphocytes and Jurkat T cells appears to be an important biologic consequence of chemokine action and more readily measurable (at nanomolar concentrations) than readouts of receptor activation such as calcium flux. It was also apparent that RANTES is the only chemokine tested to date (RANTES, MIP-1α, MIP-1β, MCP-1, MCP-3, lymphotactin) that induces as robust a response as seen in this study, although the others listed were capable of low ...
Arl1 (ARF like protein1) is a poorly understood member of ARF family small GTPases. This thesis presents an original characterization of Arl1 and its effectors. Arl1 was localized to the tans Golgi under EM. Over expression of guanine nucleotide mutants of Arl1 dramatically affects the structure and function of Golgi apparatus. Arl1-GTP was found to interact with GRIP domain of Golgins (Golgin-97, Golgin-245, GCC1 and KIAA0336). The interaction was dependent on the conserved amino acids on both switch II region of Arl1 and the GRIP domain. Collectively, the research presented in this thesis reveals Arl1 is a new regulator of Golgi structure and function and one mechanism of Arl1a??s function is that it recruits and regulates its effectors a?? GRIP domain Golgins to Golgi ...
Abbreviations: ABC, ATP-binding cassette transporter; ANGPTL3, angiopoietin-like 3; apo, apolipoprotein; ARL7, ADP-ribosylation factor-like 7; CAD, coronary artery disease; CXCR4, CXC receptor 4; EC, endothelial cell; FOXA2, forkhead box A2; GPAM, glycerol-3-phosphate acyltransferase 1; HDL, high-density lipoprotein; HDL-C, HDL-cholesterol; HMGCR, 3-hydroxy-3-methylglutaryl-CoA reductase; IL, interleukin; LDL, low-density lipoprotein; LDLR, LDL receptor; LNA, locked nucleic acid; LOX-1, oxidized LDL receptor 1; LPL, lipoprotein lipase; LPS, lipopolysaccharide; LXR, liver X receptor; Mct1, monocarboxylate transporter 1; MI, myocardial infarction; miRNA, microRNA; Mtpn, myotrophin; NFAT, nuclear factor of activated T-cells; NF-κB, nuclear factor κB; p27Kip1, cyclin-dependent kinase inhibitor 1B; p57Kip2, cyclin-dependent kinase inhibitor 1C; oxLDL, oxidized LDL; PEPCK, phosphoenolpyruvate carboxykinase; PI3K, phosphoinositide 3-kinase; PPAR, peroxisome proliferator-activated receptor; pre-miRNA, ...
D. Jones, B. Bax, S. Cockcroft; ADP-ribosylation factor GTPases in signal transduction and membrane traffic: independent functions?. Biochem Soc Trans 1 August 1999; 27 (4): 642-647. doi: https://doi.org/10.1042/bst0270642. Download citation file:. ...
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The tiny G protein Arf1 regulates Golgi traffic and it is activated by two related types of guanine nucleotide exchange factor (GEF). We validate this using the Arf1 orthologue (Arf79F) as well as the related course II Arf (Arf102F) which demonstrated a similar design of effector binding. Applying the technique towards the Arf-like G proteins Arl1 we discovered that it binds right to Sec71 the ortholog of BIG1 TNFSF14 and BIG2 via an N-terminal area. We display that in mammalian cells Arl1 is essential for Golgi recruitment of BIG2 and BIG1 however not GBF1. Thus Arl1 works to immediate a trans-Golgi-specific Arf1 GEF and therefore active Arf1 towards the trans part from the Golgi. Intro The members from the ADP ribosylation element (Arf) category of little G proteins are crucial regulators of membrane visitors and cytoskeletal systems (DSouza-Schorey and Chavrier 2006 Biopterin Gillingham and Munro 2007 Donaldson and Jackson 2011 Distinct through the other members from the Ras superfamily of ...
In addition, the arf1‐11∆arf2 mutation results in aggregated Fzo1, which is removed by overexpression of Cdc48. We identified Cdc48 as an interactor of Arf1‐GTP. Thus, a third function could be the recruitment of the AAA‐ATPase Cdc48 to mitochondria to remove Fzo1 and potentially other mitochondrial outer membrane proteins. In the wild‐type situation, Arf1 would promote recruitment of Cdc48 to mitochondria to maintain proper Fzo1 homeostasis, whereas in the mutant situation aberrant Fzo1 would accumulate because Cdc48 is recruited less efficiently. Cdc48 has a known role in the quality control of mitochondrial outer membrane proteins in yeast and worms and promotes degradation of Fzo1 (Heo et al, 2010). Consistently, the mammalian Cdc48 homologue p97 promotes ubiquitylation and proteasome‐dependent degradation of the mitofusins Mfn1 and Mfn2 (Tanaka et al, 2010). Our data are in agreement with the recently reported role of Cdc48/p97/VCP in the retro‐translocation of mitochondrial ...
Rafiq et al. demonstrate that the small G protein ARF1 and its activator, cytohesin 2 (ARNO), are required for podosome formation in macrophage-like cells and fibroblasts. Inhibition of ARNO-ARF1 signaling results in increased RhoA activity and disassembly of podosomes in a myosin-IIA-dependent fashion. In fibroblasts that normally do not form podosomes, constitutively active ARF1 induces actin-rich puncta associated with sites of matrix degradation, putative precursors of podosomes. ...
Cell migration is an orchestrated and highly coordinated multi-step process that is central to the development and maintenance of multicellular organisms. Dysregulated migration however, is associated with pathological states such as tumor formation and metastasis; thus a clear understanding of the molecular mechanisms that drive this process is critical to the development of counteracting therapeutics. Cell migration and adhesion-dependent cell spreading share a number of features. For example, both processes rely on the activation of mechanisms for the coordinated spatial and temporal assembly/disassembly of focal adhesions, as well as mechanisms controlling actin rearrangements and directed vesicular trafficking. Actin remodeling and vesicular trafficking events are in turn, implicated functions of a variety of small GTPases of the Ras superfamily, which include the Rho and Arf subfamilies. Thus towards efforts of further characterizing the molecular pathways that drive cell spreading, I pursued aims
Two closely related human Arls, Arl8a and Arl8b, were found to localise to lysosomes in mammalian cells. conventionally, membrane binding of Arf and Arl proteins is mediated by both an N-terminal myristoyl group and an N-terminal amphipathic helix that are inserted into the lipid bilayer upon activation of the GTPase. Arl8 GPTases lack myristolylation sites, and examination of the N-terminus of Arl8b revealed that it contains an acetyl group instead, and this acetylated methionine is necessary for its lysosomal location. Lysosomes of cells overexpressing Arl8b move more frequently, suggesting a role for Arl8a and Arl8b as positive regulators of lysosomal transport. Arl4a, Arl4c and Arl4d are very similar in sequence and were found to act in a pathway upstream of Arf6, Arf6 is a regulator of key processes at the plasma membrane, such as endocytosis, actin dynamics and cell adhesion. One of the major activators of Arf6 is the exchange factor ARNO (Arf nucleotide binding site opener). In order to ...
... Arf 6 Activation Assay Kit bases on the configuration-specific anti-Arf 6-GTP monoclonal antibody to measure the active Arf 6-GTP levels, either from cell extracts or from in vitro GTPγS loading Arf 6 activation assays. Briefly, anti-active Arf 6 mouse monoclonal antibody will be incubated with cell lysates containing Arf 6-GTP. The bound active Arf 6 will then be pulled down by protein A/G agarose. The precipitated active Arf 6 will be detected by immunoblot analysis using an anti-Arf 6 rabbit polyclonal antibody, respectively. ...
COPⅠ囊泡:最初研究者利用三磷酸鳥苷(GTP)衍生物GTPγS(一種富含高爾基體膜的細胞質與抗水解的GTP衍生物)共培養時,發現高爾基體池之間存在一種囊泡轉運結構[9](後來在真核細胞中也證實此結構的存在[10])。除了脂質成分外,參與此囊泡形成的成份還有7種外被體蛋白(即外被體α、β、β′、γ、δ、ε、ζ)。這些外被體蛋白相互作用形成的復合物就是COPⅠ囊泡[11][12]。亞單位α、β′、ε在結構上與網格蛋白及COPⅡ囊泡的外層組分具有較高的一致性,形成復合物的內層組分稱為B亞復合物(主要負責與靶蛋白結合),而亞單位β、γ、δ、ζ 與網格蛋白及COPⅡ囊泡的內層組分相似,形成復合物的內層組分稱為F亞復合物,該亞復合物主要負責與靶蛋白結合,並且直接與COPⅠ囊泡形成的招募者ADP核糖基化因子(英语:ADP ribosylation factor)(ADP ribosylation ...
Arf-like protein 1 (Arl1) is a member of the Arf family of regulatory GTPases, within the Ras superfamily of GTPases, and with highly conserved orthologs throughout eukaryotes. Arl1 is essential for early embryonic development in Drosophila and in Caenorhabditis elegans. Arl1 is most similar in primary sequence, cellular location, and function (regulation of membrane traffic) to Arf1-6 and even shares several common binding partners. In addition to its function in membrane traffic at the Golgi/trans-Golgi network, there are reports indicating a possible role for Arl1 in ion homeostasis in yeast. ...
Other than a role for Ca2+ store depletion, the molecular mechanisms that regulate antigen-stimulated Ca2+ influx into mast cells are not well-understood. The observation that CT dramatically enhances 45Ca2+ influx into RBL-2H3 cells suggests that this reagent might be a useful tool to study the Ca2+ entry pathway (Narasimhan et al., 1988). That CT amplifies both antigen-evoked ICRAC and 45Ca2+ influx to a similar extent bolsters the idea that CRAC channels are a major pathway for FcεRI-mediated Ca2+ uptake into RBL-2H3 mast cells (Zhang and McCloskey, 1995).. Two hypotheses to explain the effect of CT on 45Ca2+ influx are immediately testable by patch clamping. First, it is possible that CT activates Cl− or K+ channels, and thereby increases the electrical force propelling Ca2+ entry. This indirect mechanism cannot explain the enhancement of Ca2+ influx currents that we observed, because voltage-clamp measurements eliminate any difference in membrane potential between control and CT-treated ...
CONTACT: [email protected] GENBANK UPDATE: 22 July 1997 28 unique yeast ORFs ORF NAME LOCUS PRODUCT/DESCRIPTION -------- ----- ---------------------------------------- YFL039C ACT1 Actin YDL192W ARF1 ADP-ribosylation factor YDL137W ARF2 ADP-ribosylation factor 2 YJR121W ATP2 F(1)F(0)-ATPase complex beta subunit, mitochondrial YER177W BMH1 Homolog of mammalian 14-3-3 proteins YLR229C CDC42 member of the Rho subfamily of Ras-like proteins YER133W GLC7 protein phosphatase type I YLR293C GSP1 GTP-binding protein YOR185C GSP2 GTP-binding protein YBR009C HHF1 Histone H4 (HHF1 and HHF2 code for identical proteins) YNL030W HHF2 Histone H4 (HHF1 and HHF2 code for identical proteins) YBR010W HHT1 Histone H3 (HHT1 and HHT2 code for identical proteins) YNL031C HHT2 Histone H3 (HHT1 and HHT2 code for identical proteins) YDR224C HTB1 Histone H2B (HTB1 and HTB2 code for nearly identical proteins) YBL002W HTB2 Histone H2B (HTB1 and HTB2 code for nearly identical proteins) YBL087C RPL17A Ribosomal protein ...
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A careful structural analysis suggested that four of these residues may have crucial functions in the specificity of the ARF6-JIP4 interaction. First, ARF6‐Thr 79 at the C‐terminus of switch II, which makes a double hydrogen bond with Lys 417 and Asn 418 of JIP4, is replaced by a glutamine residue in ARF1 (Gln 83). Owing to its longer side chain, ARF1‐Gln 83 cannot make an hydrogen bond with Lys 417 and Asn 418 from JIP4 because of steric hindrance (Figure 6B). Second, ARF6‐Thr 53 at the interswitch region does not make any direct contact with JIP4‐LZII, but in ARF1 it is replaced by the longer and negatively charged Glu 57 residue that would face Asp 432 of JIP4, generating charge repulsion (Figure 6C). Note that this charge repulsion would be more important in the case of binding to JIP3 with a glutamate residue corresponding to Asp 432 of JIP4 (Figure 2B). Thus, ARF1‐Glu 57 and ARF1‐Gln 83 should induce charge repulsion and/or steric hindrance, providing an explanation for the ...
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Complete information for ARF6 gene (Protein Coding), ADP Ribosylation Factor 6, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Complete information for ARL6IP6 gene (Protein Coding), ADP Ribosylation Factor Like GTPase 6 Interacting Protein 6, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
ARFGAP3 antibody, C-term (ADP-ribosylation factor GTPase activating protein 3) for WB. Anti-ARFGAP3 pAb (GTX89988) is tested in Mouse samples. 100% Ab-Assurance.
Homo sapiens golgi associated, gamma adaptin ear containing, ARF binding protein 1 (GGA1), transcript variant 2, mRNA. (H00026088-R01) - Products - Abnova
ADP-ribosylation factor, GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the ...
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
Arf1 antibody (ADP-ribosylation factor 1) for IHC-P, WB. Anti-Arf1 pAb (GTX124347) is tested in Zebrafish samples. 100% Ab-Assurance.
BFA induces the ADP-ribosylation of BARS-50 and GAPDH in permeabilized cells. (A) RBL cells were permeabilized with 3 U/ml SLO and exposed to 10 μg/ml BFA
Being in the state playoffs is something special at Eisenhower.``It`s something the kids can brag about forever,`` said Eisenhower coach Jim Cygan. ``I told them on the bus ride home (after
A synthetic N-myristoylated peptide corresponding to the amino-terminal domain of ADP-ribosylation factor 1 (ARF1) markedly increases, in a cell-free system using post-nuclear supernatant from PC12 cells, the biogenesis of constitutive secretory vesicles and immature secretory granules from the trans-Golgi network (TGN). The related N-myristoylated ARF4 peptide only weakly stimulates, and the non-myristoylated ARF1 and ARF4 peptides inhibit, the biogenesis of these secretory vesicles. In a modified cell-free system using TGN membranes, coatomer-depleted cytosol supports the biogenesis of TGN-derived secretory vesicles to the same extent as control cytosol. These results suggest a role for ARF1, but not the COP I coat, in secretory vesicle biogenesis from the TGN, possibly via the activation of phospholipase D.
FUNCTION: Guanine nucleotide-exchange factor (GEF) required for the formation or budding of transport vesicles from the ER. This function involves the cytoplasmic domain of the protein, which is thought to interact with the small GTP-binding protein SAR1. Required for autophagy. MISCELLANEOUS: In the process of transport, SEC12 itself may migrate to the Golgi apparatus and function in subsequent transport events. MISCELLANEOUS: Present with 6160 molecules/cell in log phase SD medium ...
Arginine adenosine-5′-diphosphoribosylation (ADP-ribosylation) is an enzyme-catalyzed potentially reversible posttranslational changes where the ADP-ribose moiety is transferred from NAD+ towards the guanidino CHR2797 moiety of arginine. proteins with binding companions e.g. toxin-catalyzed ADP-ribosylation of actin at R177 blocks actin polymerization sterically. In case there is the nucleotide-gated P2X7 ion route ADP-ribosylation at R125 near the ligand-binding site causes route Rabbit Polyclonal to SF3B3. gating. Arginine-specific ADP-ribosyltransferases (ARTs) bring a quality R-S-EXE theme that distinguishes these enzymes from structurally related enzymes which catalyze ADP-ribosylation of additional amino acid part chains DNA or little substances. Arginine-specific ADP-ribosylation could be inhibited by little molecule arginine analogues such as for example CHR2797 agmatine or meta-iodobenzylguanidine (MIBG) which themselves can serve as focuses on for arginine-specific ARTs. ...
Summary Arl (Arf-like) proteins, GTP-binding proteins of the Ras superfamily, are molecular switches that cycle between a GDP-bound inactive and GTP-bound active state. There are 16 members of the Arl subfamily in the human genome whose basic mechanistic function is unknown. The interactome of Arl2/3 includes proteins involved in retinopathies and other ciliary diseases such as Leber¿s Congenital Amaurosis (LCA) and kidney diseases such as nephronophthisis. Arl6 has been found mutated in Bardet Biedl Syndrome, another pleiotropic ciliary disease. In the proposed interdisciplinary project I want to explore the function of the protein network of Arl2/3 and Arl6 by a combination of biochemical, biophysical and structural methods and use the knowledge obtained to probe their function in live cells. As with other subfamily proteins of the Ras superfamily which have been found to mediate similar biological functions I want to derive a basic understanding of the function of Arl proteins and how it ...
ADP ribosylation factor 1 (Arf1). Arl1 subfamily. Arl1 (Arf-like 1) localizes to the Golgi complex, where it is believed to recruit effector proteins to the trans-Golgi network. Like most members of the Arf family, Arl1 is myristoylated at its N-terminal helix and mutation of the myristoylation site disrupts Golgi targeting. In humans, the Golgi-localized proteins golgin-97 and golgin-245 have been identified as Arl1 effectors. Golgins are large coiled-coil proteins found in the Golgi, and these golgins contain a C-terminal GRIP domain, which is the site of Arl1 binding. Additional Arl1 effectors include the GARP (Golgi-associated retrograde protein)/VFT (Vps53) vesicle-tethering complex and Arfaptin 2. Arl1 is not required for exocytosis, but appears necessary for trafficking from the endosomes to the Golgi. In Drosophila zygotes, mutation of Arl1 is lethal, and in the host-bloodstream form of Trypanosoma brucei, Arl1 is essential for viability. ...
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Intracellular transport is accomplished by the regulated formation of membrane‐bound carriers that shuttle between the different intracellular membrane compartments of the secretory pathway (Rothman and Wieland, 1996; Schekman and Orci, 1996). Studies of the endoplasmic reticulum (ER) and the Golgi complex, that have provided much of our current understanding of how intracellular transport is regulated, suggest that a cytosolic coat protein complex, COPI, attaches to membranes to initiate the formation of transport vesicles (Orci et al., 1989). These coated vesicles then target to another compartment where COPI must be released to the cytosol before the vesicles can fuse with their target compartment (Orci et al., 1989).. Recruitment of COPI to membranes is regulated by a small GTPase named ADP‐ribosylation factor 1 (ARF1). Activation of ARF1 by its binding of GTP (ARF1‐GTP) recruits COPI from the cytosol to membranes (Donaldson et al., 1992; Palmer et al., 1993), while ARF1 inactivation ...
Hong Xu wrote: , , Dear Colleagues: , , Our group is using the Clontech s HeLa cDNA library in a yeast , two-hybrid screening with the bait fused to the GAL4AD and the yeast , strain CG 1945. Many of the clones sequenced show one of the following , sequences, including ITBA2 (whatever it is, since almost no references , can be found in Medline), ribosomal s20, transketolase, Ring12 (probably , a proteasome subunit), ADP ribosylation factor, etc., with those listed , above showing up most frequently. We would like to know whether or not , these factors have also been found in somebody else two-hybrid , screenings using the same Clontech HeLa library. , , You are welcome to post your answer to our question in this newsgroup. , Or you can call me at 973-781-7987. , , Thank you very much. , , Richard Dear Richard, try this URL and you find an overview of the most common trash found in two hybrid screens: http://www.fccc.edu/research/labs/golemis/main_false.html Hope this helps, Ricky ...
This gene encodes a phosphoinositide binding protein containing ARF-GAP, RHO-GAP, RAS-associating, and pleckstrin homology domains. The ARF-GAP and RHO-GAP domains cooperate in mediating rearrangements in the cell cytoskeleton and cell shape. It is a specific PtdIns(3,4,5)P3/PtdIns(3,4)P2-stimulated Arf6-GAP protein. An alternatively spliced transcript has been found for this gene, but its biological validity has not been determined ...
ADP-ribosylation Factor GTPase Activating Protein (ARF GAP); Involved In ER-Golgi Transport; Required For Prospore Membrane Formation; Regulates Phospholipase Spo14p; Shares Functional Similarity With Glo3p; GCS1 Has A Paralog, SPS18, That Arose From The Whole Genome Duplication
Lymphocytes express a flurry of nucleotide-metabolizing ectoenzymes, including ENTPDases (CD39), pyrophosphatases (CD203), 5′-nucleotidase (CD73), NADases (CD38, CD157), and ARTs (37, 38), the function of which has puzzled investigators. One hypothesis holds that these enzymes catabolize ATP, NAD, and other nucleotides released from dying cells in inflamed tissues to recycle nucleotides by providing precursors for uptake by proliferating lymphocytes (37). Another hypothesis proposes that these enzymes control the availability of ATP and its metabolites ADP, AMP, and adenosine as ligands for specific receptors (P2X and P2Y family of purinergic receptors, P1 family of adenosine receptors) (24, 28, 29). In this study, we explore the hypothesis that CD38 may similarly affect the availability of NAD as an extracellular signaling molecule by controlling the concentration of NAD and the duration of its presence as a substrate for ART2-catalyzed ADP-ribosylation of cell surface proteins.. Our results ...
Human ARL1 partial ORF ( NP_001168, 72 a.a. - 181 a.a.) recombinant protein with GST-tag at N-terminal. (H00000400-Q01) - Products - Abnova
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said: u guys r so Imature. I believe her 2 i bet EVERYONE here that DOESNT believe her would fangirl and they r just NORMAL boys thats it. Just treat them like normal people they shouldnt be treated ANY different then us. I bet if u asked them they would say that they wanna be treated like normal people. And maybe if u go 2 a concert u might get there # and then u would wanna brag about it i know i would. So keep ur nasty comments 2 ur self. Just leave her alone! I want there #s 2 but she probably wont give it 2 me(i REALLY want it) but keep ur nasty comments 2 ur selves i bet if ANYONE from one direction saw these comments they wouldnt be happy with these comments except the nice ones ...
No. You are not. But, were odd like that. We also yell, No one expects the Spanish Inquisition! whenever anybody says that they didnt expect something. Im not sure these are things to brag about.. ...
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CIN85 is a multidomain adaptor protein involved in Cbl-mediated down-regulation of epidermal growth factor (EGF) receptors. CIN85 src homology 3 domains specifically bind to a proline-arginine (PxxxPR) motif in Cbl, and this association seems to be important for EGF receptor endocytosis. Here, we report identification of novel CIN85 effectors, all containing one or more PxxxPR motifs, that are indispensable for their mutual interactions. These effectors include phosphatidyl-inositol phosphatases SHIP-1 and synaptojanin 2B1, Arf GTPase-activating proteins ASAP1 and ARAP3, adaptor proteins Hip1R and STAP1, and a Rho exchange factor, p115Rho GEF. Acting as a molecular scaffold, CIN85 clusters its effectors and recruits them to high-molecular-weight complexes in cytosolic extracts of cells. Further characterization of CIN85 binding to ASAP1 revealed that formation of the complex is independent on cell stimulation. Overexpression of ASAP1 increased EGF receptor recycling, whereas ASAP1 containing ...
The ADP-ribosylation factor (Arf) proteins are GTP-binding proteins that control vesicular transport and remodeling of the actin cytoskeleton (DSouza-Schorey and Chavrier, 2006; Gillingham and Munro, 2007; Donaldson and Jackson, 2011). These proteins cycle between active GTP-bound and inactive GDP-bound forms with distinct functions. The tight control of this cycle relies on two key families of proteins. Arf-Guanine nucleotide exchange factors (Arf-GEFs) catalyze the exchange of GDP, whereas Arf-GTPase activating proteins (Arf-GAPs) induce hydrolysis of GTP bound to Arf proteins. Besides their catalytic functions, both GEFs and GAPs can serve as scaffold for numerous effector proteins and can thus regulate cellular physiology in an Arf-independent manner (Inoue and Randazzo, 2007; Randazzo et al., 2007).. In humans, 31 predicted ArfGAPs have been classified into ten subfamilies (Kahn et al., 2008). In addition to a GAP domain, four subfamilies (ASAP, ACAP, AGAP and ARAP), comprising 20 proteins ...
The intracellular signaling pathways through ADP-ribosylation factors (Arfs) of the small GTPase family control cell morphological changes by regulating membrane components and/or cytoskeletal protein dynamics. We previously reported that cytohesin-2 (CYTH2), an Arf-guanine-nucleotide exchange factor (GEF), binds to the cytoskeletal scaffold protein paxillin through C-terminal region of CYTH2 and promotes the migration of mouse 3T3-L1 fibroblasts. In mammals, CYTH family GEFs are composed of four subfamilies. Among them, CYTH2 and CYTH3 are widely expressed in tissues and it remains to be clarified to determine whether they have specific biochemical and cellular functions or are redundant. Here, we show that the C-terminal short polybasic region of CYTH2 is necessary and sufficient for binding to paxillin to mediate cell migration. Although 3T3-L1 cells primarily express CYTH2 and CYTH3 of four CYTH family members, neither knockdown of CYTH3 by the specific siRNA nor expression of its C-terminal region
ARF GAP Lis a kind of important regulator of introcellular transport. Recently, a novel human gene has been found from a cDNA library of second trimester human fetal liver. The amino acid sequence encoded by the novel gene has 32% similarity to rat ARF1 GAP, was thus termed as ARFGAP3. Functional studies of the new gene were performed. The full-length cDNA of ARFGAP3 was amplified from the human total placenta RNA by RT-PCR technique, then subcloned into pGEM-T vector and sequenced. The RNA Master blot and multiple tissue Northern blot analysis were used to define the expression profile and the transcript size of ARFGAP3 in human tissues. It was shown that ARFGAP3 was strongly expressed in glands and testis and that ARFGAP3 mRNA existed as only one kind of transcript of 2.7 kb in various human tissues. Then, the expression and purification of the recombinant human ARFGAP3 (rhARFGAP3) were performed. It was demonstrated that rhARFGAP3 exhibited strong GTPase-activating protein ( GAP) activity ...
(His)6-GBF1 is a BFA-resistant ARF-GEF. (A) Fractions enriched in (His)6-GBF1 display a GEF specific for ARFs. Identical volumes (5 μl) of the 50 mM imidazole
VHS domains are found at the N-termini of select proteins involved inintracellular membrane trafficking. We have determined the crystalstructure of the VHS domain of the human Tom1 (target of myb 1) protein to1.5 A resolution. The domain consists of eight helices arranged in asuperhelix. The surface of the domain has two main features: (1) a basicpatch on one side due to several conserved positively charged residues onhelix 3 and (2) a negatively charged ridge on the opposite side, formed byresidues on helix 2. We compare our structure to the recently obtainedstructure of tandem VHS-FYVE domains from Hrs [Mao, Y., Nickitenko, A.,Duan, X., Lloyd, T. E., Wu, M. N., Bellen, H., and Quiocho, F. A. (2000)Cell 100, 447-456]. Key features of the interaction surface between theFYVE and VHS domains of Hrs, involving helices 2 and 4 of the VHS domain,are conserved in the VHS domain of Tom1, even though Tom1 does not have aFYVE domain. We also compare the structures of the VHS domains of Tom1 andHrs to the ...
The American Radio Relay League (ARRL) is the national association for amateur radio, connecting hams around the U.S. with news, information and resources.
DI-fusion, le Dépôt institutionnel numérique de lULB, est loutil de référencementde la production scientifique de lULB.Linterface de recherche DI-fusion permet de consulter les publications des chercheurs de lULB et les thèses qui y ont été défendues.
This patent search tool allows you not only to search the PCT database of about 2 million International Applications but also the worldwide patent collections. This search facility features: flexible search syntax; automatic word stemming and relevance ranking; as well as graphical results.
Arno Kwade is the author of this article in the Journal of Visualized Experiments: Development of an Experimental Setup for the Measurement of the Coefficient of Restitution under Vacuum Conditions
Inquires can be sent to: [email protected] For further information, please visit: https://www.arl.army.mil/opencampus/ARLSouth. ...
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Brefeldin A-inhibited guanine nucleotide-exchange protein 3 (BIG3) has been identified recently as a novel regulator of estrogen signalling in breast cancer cells. Despite being a potential target for new breast cancer treatment, its amino acid sequence suggests no association with any well-characterized protein family and provides little clues as to its molecular function. In this paper, we predicted the structure, function and interactions of BIG3 using a range of bioinformatic tools. Homology search results showed that BIG3 had distinct features from its paralogues, BIG1 and BIG2, with a unique region between the two shared domains, Sec7 and DUF1981. Although BIG3 contains Sec7 domain, the lack of the conserved motif and the critical glutamate residue suggested no potential guaninyl-exchange factor (GEF) activity. Fold recognition tools predicted BIG3 to adopt an α-helical repeat structure similar to that of the armadillo (ARM) family. Using state-of-the-art methods, we predicted interaction sites
The adipocyte-derived protein adiponectin is highly heritable and inversely associated with risk of type 2 diabetes mellitus (T2D) and coronary heart disease (CHD). We meta-analyzed 3 genome-wide association studies for circulating adiponectin levels (n = 8,531) and sought validation of the lead single nucleotide polymorphisms (SNPs) in 5 additional cohorts (n = 6,202). Five SNPs were genome-wide significant in their relationship with adiponectin (P≤5×10−8). We then tested whether these 5 SNPs were associated with risk of T2D and CHD using a Bonferroni-corrected threshold of P≤0.011 to declare statistical significance for these disease associations. SNPs at the adiponectin-encoding ADIPOQ locus demonstrated the strongest associations with adiponectin levels (P-combined = 9.2×10−19 for lead SNP, rs266717, n = 14,733). A novel variant in the ARL15 (ADP-ribosylation factor-like 15) gene was associated with lower circulating levels of adiponectin (rs4311394-G, P-combined = 2.9×10−8, n = ...
Small GTPases largely control membrane traffic, which is essential for the survival of all eukaryotes. Among the small GTP-binding proteins, ARF1 (ADP-ribosylation factor 1) and SAR1 (Secretion-Associated RAS super family 1) are commonly conserved among all eukaryotes with respect to both their functional and sequential characteristics. The ARF1 and SAR1 GTP-binding proteins are involved in the formation and budding of vesicles throughout plant endomembrane systems. ARF1 has been shown to play a critical role in COPI (Coat Protein Complex I)-mediated retrograde trafficking in eukaryotic systems, whereas SAR1 GTPases are involved in intracellular COPII-mediated protein trafficking from the ER to the Golgi apparatus. This review offers a summary of vesicular trafficking with an emphasis on the ARF1 and SAR1 expression patterns at early growth stages and in the de-etiolation process.
The molecular machinery responsible for the generation of transport carriers moving from the Golgi complex to the plasma membrane relies on a tight interplay between proteins and lipids. Among the lipid-binding proteins of this machinery, we previously identified the four-phosphate adaptor protein FAPP2, the pleckstrin homology domain of which binds phosphatidylinositol 4-phosphate and the small GTPase ARF1. FAPP2 also possesses a glycolipid-transfer-protein homology domain. Here we show that human FAPP2 is a glucosylceramide-transfer protein that has a pivotal role in the synthesis of complex glycosphingolipids, key structural and signalling components of the plasma membrane. The requirement for FAPP2 makes the whole glycosphingolipid synthetic pathway sensitive to regulation by phosphatidylinositol 4-phosphate and ARF1. Thus, by coupling the synthesis of glycosphingolipids with their export to the cell surface, FAPP2 emerges as crucial in determining the lipid identity and composition of the plasma
Issuu is a digital publishing platform that makes it simple to publish magazines, catalogs, newspapers, books, and more online. Easily share your publications and get them in front of Issuus millions of monthly readers. Title: The Brag #492, Author: Peer Group Media / Furst Media, Name: The Brag #492, Length: 56 pages, Page: 1, Published: 2012-12-10
Inhibitors of the Hedgehog (Hh) pathway transducer Smoothened (Smo) have been approved for cancer treatment, but Smo mutations often lead to tumor resistance and it remains unclear how Smo is regulated. In this study, we identified the small GTPase Arl13b as a novel partner and regulator of Smo. Arl13b regulated Smo stability, trafficking, and localization, which are each crucial for Hh signaling. In gastric cancer cells, Arl13b stimulated proliferation, migration, and invasion in vitro and in vivo. In clinical specimens of gastric cancer, Arl13b expression correlated strongly with tumor size and depth of invasion; patients with high levels of Arl13b had a poor prognosis. Our results show how Arl13b participates in Hh pathway activation in gastric cancer. Cancer Res; 77(15); 1-14. ©2017 AACR. ...
You went where?! Thats the question that separates tourists from traveler-and the one we secretly love to be asked. Here are ten adventures that will help you make sure you always have something to brag about.
Filediva lets you search for shared files from various file hosting sites like: Uploaded.to, Rapidgator.net, Netload.in, 4shared.com, Extabit.com, Turbobit.net, Mediafire.com, Rapidgator.net, Netload.in, 4shared.com, Extabit.com, Turbobit.net, Mediafire.com and many others. Last searches: Hugo_2011_dvdrip_rarf+2014+u629678+RapidmovieGianna+Michaels+ICP2+btswwwmp3prcomSombat+Phi+2012+P4+mp4elena_matureFire+Birds+1990+DVDRip+XViD-NoGrp+rarA+novinha+do+Col%C3%A9gio+Universo+de+Bel%C3%A9m+%E2%80%93+Caiu+na+net+mp4MM11+SamanthaSaint+mp4Adobe.Acrobat.v9.3.Pro.Mualexa_benson_mp4_wmv_aviTetrahymena_thermophilaColt+Pounds+Kellan+FHD.mp4the_playmates_don039t_go_homebbe12444+3000+mp4Scooby+Doo+The+Mystery+BeMEN+LandonM+DatoF+360p+mp4zack_petrocSexyTamara+-+Dirty+Pizza+Express+Sahne+Satt+flvRains_from_the_ashes ...
Wednesday is Gal Pal golf day. We teed off this morning at 10:00 am, off the back nine. The 11th hole is over the water and always gives me trouble, but today I parred it! Everything seemed to be working today, driving, fairway shots, chipping and putting. I was feelin real good until the 14th hole when I hit my 2nd shot into the creek, then my 4th shot into the sand for a 9 for the hole, ouch! Back on track on the next hole I shot a bogey (over water), double bogey, birdie!, and bogey, to end up with a personal best, a 47! Ive been trying to break 50 for the longest time and did it today, hoorah for me ...
so, i just bought a Kustom Tube 12. 12 watts, 8 inch Celestion speaker, all tube (i think), and it sounds amazing. of course i am comparing it to my o...
Buy online, view images and see past prices for Arno Malinowski for Georg Jensen Ltd, a Danish. Invaluable is the worlds largest marketplace for art, antiques, and collectibles.
hook the teachers on blogging in the classroom. Not to brag, but I really think we did a pretty good job! Lori uses EduBlogs with her kids and I use KidBlog, so between the two of us we had quite a bit of information to share about each platform. About half of the session time was spent allowing the teachers to play around with the blogging platforms. It was a great day ...
I dont know if the NAET treatments are finally working or Jasper has just grown out of some of his "issues" but he has been such a good boy lately that you must allow me to brag!. This past Sunday …. Filed under: allergies, NAET by ...
We recently reported that brefeldin A-inhibited guanine nucleotide-exchange proteins 3 (BIG3) binds Prohibitin 2 (PHB2) in cytoplasm, thereby leading to a reduction of function of the PHB2 growth suppressor in the nuclei of breasts tumor cells. PHB2 nuclear transfer may offer restorative strategies for managing Elizabeth2/Emergency room signs in breasts tumor cells. Introduction Prohibitin 1 and 2 (PHB and buy 808118-40-3 PHB2) proteins are highly conserved in eukaryotic cells and exhibit diverse subcellular localization with different functions [1C3]. These molecules are primarily observed in inner mitochondrial membranes via their buy 808118-40-3 N-terminal transmembrane domain but are also present in several other localizations such as the cytosol, endoplasmic reticulum, nucleus, and plasma membrane [1]. Both proteins form hetero-oligomeric ring structures in the inner mitochondrial membrane and function as chaperones buy 808118-40-3 that maintain mitochondrial integrity and stabilize ...
Common genetic variants at the ARL15 locus are associated with plasma adiponectin, insulin and HDL cholesterol concentrations, obesity, and coronary atherosclerosis. The ARL15 gene encodes a small GTP-binding protein whose function is currently unknown. In this study adipocyte-autonomous roles for ARL15 were investigated using conditional knockdown of Arl15 in murine 3T3-L1 (pre)adipocytes. Arl15 knockdown in differentiated adipocytes impaired adiponectin secretion but not adipsin secretion or insulin action, while in preadipocytes it impaired adipogenesis. In differentiated adipocytes GFP-tagged ARL15 localized predominantly to the Golgi with lower levels detected at the plasma membrane and intracellular vesicles, suggesting involvement in intracellular trafficking. Sequencing of ARL15 in 375 severely insulin resistant patients identified four rare heterozygous variants, including an early nonsense mutation in a proband with femorogluteal lipodystrophy and non classical congenital adrenal ...
ADP-ribosylation is the addition of one or more ADP-ribose moieties to a protein. It is a reversible post-translational modification that is involved in many cellular processes, including cell signaling, DNA repair, gene regulation and apoptosis. Improper ADP-ribosylation has been implicated in some forms of cancer. It is also the basis for the toxicity of bacterial compounds such as cholera toxin, diphtheria toxin, and others. The first suggestion of ADP-ribosylation surfaced during the early 1960s. At this time, Pierre Chambon and coworkers observed the incorporation of ATP into hen liver nuclei extract. After extensive studies on the acid insoluble fraction, several different research laboratories were able to identify ADP-ribose, derived from NAD+, as the incorporated group. Several years later, the enzymes responsible for this incorporation were identified and given the name poly (ADP-ribose) polymerase. Originally, this group was thought to be a linear sequence of ADP-ribose units ...
TABLE-US-00004 TABLE 4 Topotecan Predictor Set of Gene Expression Profiles Representative Probe Set ID Gene Title Gene Sym UniGene Public ID 200050_at zinc finger protein 146 /// zinc finger ZNF146 301819 NM_007145 protein 146 200065_s_at ADP-ribosylation factor 1 /// ADP- ARF1 286221 AF052179 ribosylation factor 1 200077_s_at ornithine decarboxylase antizyme 1 /// OAZ1 446427 D87914 ornithine decarboxylase antizyn 200710_at acyl-Coenzyme A dehydrogenase, very ACADVL 437178 NM_000018 long chain 200717_x_at ribosomal protein L7 RPL7 421257 NM_000971 200819_s_at ribosomal protein S15 RPS15 406683 NM_001018 200839_s_at cathepsin B CTSB 520898 NM_001908 200949_x_at ribosomal protein S20 RPS20 8102 NM_001023 201193_at isocitrate dehydrogenase 1 (NADP+), IDH1 11223 NM_005896 soluble 201219_at C-terminal binding protein 2 /// CTBP2 /// I 501345 AW269836 LOC440008 201381_x_at calcyclin binding protein CACYBP 508524 AF057356 201434_at tetratricopeptide repeat domain 1 TTC1 519718 NM_003314 201482_at ...
Deregulation of translational control can promote cellular transformation. Protein synthesis and the expression of components of the translation machinery are elevated in cancers and contribute to tumorigenesis (1-5, 7). Here, we show that nuclear ErbB2 promotes binding of RNA Pol I to rDNA, co-occupies the rRNA gene with β-actin and RNA Pol I, and stimulates rRNA production and protein translation independently of traditional ErbB2 downstream PI3-K and ERK signalings, suggesting that nuclear ErbB2 may contribute to oncogenesis by upregulating total cellular translation. rRNA synthesis by RNA Pol I plays a critical role in production of mature ribosomes that are central protein synthesis machinery of the cells. Perturbation of RNA Pol I activity as well as rRNA and protein biosynthesis (i.e., translation control) by oncoproteins such as Myc or tumor suppressors p53, RB, and ADP ribosylation factor has been reported to be associated with tumor development (1, 2, 7-10). The capability of Myc to ...
ADP-Ribosylation Factor 1 is involved in regulating intracellular transport by modulating the interaction of Coat Proteins with Organelle Membranes in the early Secretory Pathway. It is a component of Coat Protein Complex I. This enzyme was formerly listed as EC 3.6.1.47 ...
Small GTP-binding proteins (G proteins) are monomeric G proteins with a low molecular weight of 20 to 40 kDa. A small G protein acts as a molecular switch that cycles between inactive GDP-bound and active GTP-bound forms. Thus far, ,100 small G proteins have been identified in eukaryotes from yeast to humans. The small G proteins in this superfamily are structurally classified into ≥5 families: the Ras, Rho, Rab, Sar/Arf, and Ran families. In general, the Ras family mainly regulates gene expression, the Rho family regulates both cytoskeletal reorganization and gene expression, the Rab and Sar1/Arf families regulate intracellular vesicle trafficking, and the Ran family regulates nucleocytoplasmic transport and microtubule organization during the cell cycle.1. Multiple downstream effectors of small G proteins, some of them being protein kinases, have been identified. Ras mediates its effect on cell proliferation mainly by activation of its effector Raf to initiate the mitogen-activated protein ...
Two widely expressed mammalian phosphatidylcholine (PC)-specific phospholipases D (PLD), PLD1 and PLD2, have been identified. Recombinantly expressed PLD2 has high basal activity and is insensitive to GTP-binding protein activators of PLD1 [Colley, W. C., et al. (1997) Curr. Biol. 7, 191-201]. To investigate the regulation of PLD2 we isolated PLD2, from mouse brain by immunoaffinity chromatography. The native and recombinant proteins have indistinguishable properties: PLD2 is potently activated by phosphoinositides with a vicinal 4,5-phosphate pair but is not stimulated by guanosine 5-O-(3-thio triphosphate)-activated ADP-ribosylation factor-1, Rho family GTP-binding proteins, or protein kinases C-alpha, or -beta1 ...
Evolution of tumor suppressor genes can involve a trade-off because the acquisition of certain anti-cancer characteristics diminishes the ability to regenerate damaged tissue.
PARP catalysed ADP-ribosylation is a post-translational modification involved in several physiological and pathological processes, including cellular stress. In order to visualise both Poly-, and Mono-, ADP-ribosylation in vivo, we engineered specific fluorescent probes. Using them, we show that amino-acid starvation triggers an unprecedented display of mono-ADP-ribosylation that governs the formation ... read more of Sec body, a recently identified stress assembly that forms in Drosophila cells. We show that dPARP16 catalytic activity is necessary and sufficient for both amino-acid starvation induced mono-ADP-ribosylation and subsequent Sec body formation and cell survival. Importantly, dPARP16 catalyses the modification of Sec16, a key Sec body component, and we show that it is a critical event for the formation of this stress assembly. Taken together our findings establish a novel example for the role of mono-ADP-ribosylation in the formation of stress assemblies, and link this modification ...
ADP-ribosylation of proteins occurs in many eukaryotes, and it is also the mechanism of action of a growing number of important bacterial toxins. To date, however, there is only one well-characterized ADP-ribosylation system where the ADP-ribosyltransferase and the substrate protein are both bacterial in origin, namely within the nitrogen-fixing bacterium Rhodospirillum rubrum. The present paper demonstrates the endogenous ADP-ribosylation of two proteins of Mr 32,000 and 20,000 within Pseudomonas maltophilia, a Gram-negative aerobe. The proteins have been partially purified: two apparently separate species of modified protein can be separated by ion-exchange chromatography and gel filtration (V0 and Mr 158,000 - Vi). The substrate protein(s) either has, or is co-eluted with, NAD+ glycohydrolase activity. The modification is mono-ADP-ribosyl in nature. The linkage between the acceptor amino acid and the ADP-ribose moiety is alkali-labile and stable to hydroxylamine, possibly indicating an ...
PIKE-A (PIKE-activating gene differs from PIKE-S with the addition of a 40-kDa C-terminal extension containing Arf-GAP and two ankyrin-repeat domains. and peripheral bloodstream leukocytes (4-6). Weve proven that PIKE-A is certainly coamplified with CDK4 on chromosome 12 in a number of human malignancies including sarcoma RG7112 neuroblastoma and glioblastoma (32). PIKE-A is certainly readily discovered in 12q-amplified cell lines including RMS13 rhabdomyosarcoma and OSA osteosarcoma however not in regular muscles (6). PIKE-A provides the GTPase PH ArfGAP and two Ankyrin repeats domains within PIKE-L but does not have the N-terminal proline-rich area which binds proteins 4.1N PLC-γ1 and PI3-kinase. PIKE-A particularly binds to energetic Akt and up-regulates its activity within a GTP-dependent way mediating human cancers cell invasion (32). Akt/PKB is an essential regulator of divergent cellular procedures including apoptosis proliferation fat burning capacity and differentiation. Constitutive ...
ADP-Ribosylation Factors/immunology/*metabolism, Adaptor Proteins; Vesicular Transport/immunology/*metabolism, Amino Acid Motifs/immunology, Antigens; CD/immunology, Brefeldin A/pharmacology, Carrier Proteins/immunology/metabolism, Cell Adhesion Molecules; Neuronal/immunology/*metabolism, Cell Compartmentation/drug effects/immunology, Cells; Cultured, Dexamethasone/pharmacology, Endocytosis/drug effects/physiology, Endosomes/drug effects/immunology/*metabolism, Flow Cytometry, Humans, Interleukin-4/pharmacology, Macrophages/drug effects/immunology/*metabolism, Membrane Glycoproteins/immunology/metabolism, Platelet Membrane Glycoproteins/immunology, Protein Binding/physiology, Protein Structure; Tertiary/physiology, Protein Transport/drug effects/immunology, Receptors; Immunologic/immunology, Research Support; Non-U.S. Govt, Signal Transduction/drug effects/immunology, Transferrin/metabolism, trans-Golgi Network/drug effects/*immunology/metabolism ...
Auxin is a key signaling molecule for most organogenesis and patterning processes occurring during plant development [50]. The auxin transduction pathway is mainly comprised of two transcriptional regulator families: ARFs and Aux/IAAs [37, 51]. ARFs directly bind to down-stream target genes and regulate their expression during development [52]. ARFs are also involved in the reproduction of various plant species [3, 53]. Characterization and analysis of CpARFs allowed us to reveal the mechanisms behind auxin involvement in fruit and flower development of papaya [54].. In this study, the reference genome sequence of papaya, which is relatively small in size (372 Mbp) [55], was used to identify the complete CpARF family. The number of CpARF genes was less than that in Arabidopsis (23 ARFs) [37]. Protein domain analysis provided us useful information on the biological function of ARFs. A typical ARF contains a DBD, an MR, and a CTD [37]. Aux/IAAs bind to CTDs of ARFs and form heterodimers. The ...

WikiGenes - ARL6IP6 - ADP-ribosylation factor-like 6 interacting...WikiGenes - ARL6IP6 - ADP-ribosylation factor-like 6 interacting...

Synonyms: ADP-ribosylation factor-like protein 6-interacting protein 6, AIP-6, ARL-6-interacting protein 6, Aip-6, MGC33864 ...
more infohttps://www.wikigenes.org/e/gene/e/151188.html

ARL5C (ADP-ribosylation factor-like 5C) - KOMP (Knockout Mouse Project)ARL5C (ADP-ribosylation factor-like 5C) - KOMP (Knockout Mouse Project)

ADP-ribosylation factor-like 5C. Synonyms: Arl12. Gene nomenclature, locus information, and GO, OMIM, and PMID associations are ... Vega: OTTMUSG2915 (Arl5c, ADP-ribosylation factor-like 5C)*CCDS: 25334, 25334.1*Gene Ontology: Arl5c *Mouse Phenome DB: Arl5c * ...
more infohttps://www.komp.org/geneinfo.php?geneid=23736

Myc-DDK-tagged ORF clone of Homo sapiens ADP-ribosylation factor-like 5C (ARL5C) as transfection-ready DNA - RC227851 - 10 µg -...Myc-DDK-tagged ORF clone of Homo sapiens ADP-ribosylation factor-like 5C (ARL5C) as transfection-ready DNA - RC227851 - 10 µg -...

Myc-DDK-tagged ORF clone of Homo sapiens ADP-ribosylation factor-like 5C (ARL5C) as transfection-ready DNA - 10 µg - OriGene - ... home , products , origene , myc-ddk-tagged orf clone of homo sapiens adp-ribosylation factor-like 5c (arl5c) as transfection- ... Myc-DDK-tagged ORF clone of Homo sapiens ADP-ribosylation factor-like 5C (ARL5C) as transfection-ready DNA - RC227851 - 10 µg ... TrueORF Myc-DDK-tagged ORF clone of Homo sapiens ADP-ribosylation factor-like 5C (ARL5C) as transfection-ready DNA ...
more infohttps://www.bioscience.co.uk/product~184687

Arfgef1 (untagged) - Mouse ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited) (Arfgef1), (10ug...Arfgef1 (untagged) - Mouse ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited) (Arfgef1), (10ug...

Mouse ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited) (Arfgef1), (10ug), 10 µg. ... Home » cDNA » Mouse cDNA » Arfgef1 (untagged) - Mouse ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A- ... Properties for Arfgef1 (untagged) - Mouse ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited) ( ... MC224976 Arfgef1 (untagged) - Mouse ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited) ( ...
more infohttps://www.acris-antibodies.com/cdna/mouse-cdna/arfgef1-untagged-mouse-adp-ribosylation-factor-guanine-nucleotide-exchange-factor-1-brefeldin-a-inhibited-arfgef1-10ug-mc224976.htm

Effect of protein kinase a activity on the association of ADP-ribosylation factor 1 to Golgi membranesEffect of protein kinase a activity on the association of ADP-ribosylation factor 1 to Golgi membranes

Ver más] The small GTP-binding protein ADP-ribosylation factor 1 (ARF1) is an essential component of the molecular machinery ... The small GTP-binding protein ADP-ribosylation factor 1 (ARF1) is an essential component of the molecular machinery that ... Effect of protein kinase a activity on the association of ADP-ribosylation factor 1 to Golgi membranes. ...
more infohttps://idus.us.es/xmlui/handle/11441/40671

IJMS  | Free Full-Text | ARF1 and SAR1 GTPases in Endomembrane Trafficking  in Plants | HTMLIJMS | Free Full-Text | ARF1 and SAR1 GTPases in Endomembrane Trafficking in Plants | HTML

Among the small GTP-binding proteins, ARF1 (ADP-ribosylation factor 1) and SAR1 (Secretion-Associated RAS super family 1) are ... Coatomer and dimeric ADP ribosylation factor 1 promote distinct steps in membrane scission. J. Cell Biol 2011, 194, 765-777. [ ... Overexpression of ADP-ribosylation factor 1 in human gastric carcinoma and its clinicopathological. Cancer Sci 2012, 103, 1136- ... 2. ARF1 (ADP-Ribosylation Factor 1) and SAR1 (Secretion-Associated RAS Super Family 1) GTPases. Protein coats are classified ...
more infohttp://mdpi.com/1422-0067/14/9/18181/htm

ADP ribosylation factor - wikidocADP ribosylation factor - wikidoc

ADP Ribosylation Factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily. ARF family ... Retrieved from "https://www.wikidoc.org/index.php?title=ADP_ribosylation_factor&oldid=157557" ... GTPase activating proteins (GAPs) force ARF to hydrolyze bound GTP to GDP, and Guanine nucleotide exchange factors force ARF to ...
more infohttp://wikidoc.org/index.php?title=ADP_ribosylation_factor&printable=yes

ADP-ribosylation factor-like protein 3 - DrugBankADP-ribosylation factor-like protein 3 - DrugBank

ADP-ribosylation factor-like protein 3. Details. Name. ADP-ribosylation factor-like protein 3. Kind. protein. Organism. Humans ... ADP-ribosylation factor-like protein 3. P36405. Details. Drug Relations. Drug Relations. DrugBank ID. Name. Drug group. ...
more infohttps://www.drugbank.ca/bio_entities/BE0004638

ARF4 ADP ribosylation factor 4 [Homo sapiens (human)] - Gene - NCBIARF4 ADP ribosylation factor 4 [Homo sapiens (human)] - Gene - NCBI

ADP-ribosylation factor 4. Names. ADP-ribosylation factor 2. NP_001651.1. *EC 3.6.5.1 ... ARF4 ADP ribosylation factor 4 [Homo sapiens] ARF4 ADP ribosylation factor 4 [Homo sapiens]. Gene ID:378 ... ADP ribosylation factor 4provided by HGNC. Primary source. HGNC:HGNC:655 See related. Ensembl:ENSG00000168374 MIM:601177 Gene ... found ADP-ribosylation factor (ARF) 4 is one of the putative target genes of Mir-221, and the direct binding relationship was ...
more infohttps://www.ncbi.nlm.nih.gov/gene/378

ADP-Ribosylation Factor 1 (ARF1) AntibodiesADP-Ribosylation Factor 1 (ARF1) Antibodies

ADP-RIBOSYLATION FACTOR, ADP-ribosylation factor 1, ADP-RIBOSYLATION FACTOR 1A, arf1, arf2, arf3, ATARF, ATARF1, ATARFA1A, ... ADP-ribosylation factor 1 , adp-ribosylation factor 1 , ADP-ribosylation factor domain protein 1 ... ADP-Ribosylation Factor 1 (ARF1) Antigen Profile Antigen Summary ADP-ribosylation factor 1 (ARF1) is a member of the human ARF ... Browse our anti-ADP-Ribosylation Factor 1 (ARF1) Antibodies. Full name:. anti-ADP-Ribosylation Factor 1 Antibodies (ARF1). On ...
more infohttp://www.antibodies-online.com/transition-metal-ion-homeostasis-pathway-58/arf1-antibody-206/

RCSB PDB - Protein Feature View 









 - ADP-ribosylation factor 1 - Q7KQL3 (ARF1 PLAF7)RCSB PDB - Protein Feature View - ADP-ribosylation factor 1 - Q7KQL3 (ARF1 PLAF7)

The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
more infohttp://www.rcsb.org/pdb/protein/Q7KQL3

RCSB PDB - Protein Feature View 









 - ADP-ribosylation factor 6 - P62332 (ARF6 RAT)RCSB PDB - Protein Feature View - ADP-ribosylation factor 6 - P62332 (ARF6 RAT)

The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
more infohttp://www.rcsb.org/pdb/protein/P62332

WikiGenes - ARL13B - ADP-ribosylation factor-like 13BWikiGenes - ARL13B - ADP-ribosylation factor-like 13B

Synonyms: ADP-ribosylation factor-like protein 13B, ADP-ribosylation factor-like protein 2-like 1, ARL2-like protein 1, ARL2L1 ...
more infohttps://www.wikigenes.org/e/gene/e/200894.html

ADP-ribosylation factor like GTPase 11 ELISA Kits | Biocompare.comADP-ribosylation factor like GTPase 11 ELISA Kits | Biocompare.com

Compare ADP-ribosylation factor like GTPase 11 ELISA Kits from leading suppliers on Biocompare. View specifications, prices, ... ADP-ribosylation factor like GTPase 11 ELISA Kits. The ELISA (enzyme-linked immunosorbent assay) is a widely used application ... Your search returned 24 ADP-ribosylation factor like GTPase 11 ELISA ELISA Kit across 2 suppliers. ...
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ADP ribosylation factor like GTPase 13A ELISA Kits | Biocompare.comADP ribosylation factor like GTPase 13A ELISA Kits | Biocompare.com

Compare ADP ribosylation factor like GTPase 13A ELISA Kits from leading suppliers on Biocompare. View specifications, prices, ... ADP ribosylation factor like GTPase 13A ELISA Kits. The ELISA (enzyme-linked immunosorbent assay) is a well-established ... Your search returned 26 ADP ribosylation factor like GTPase 13A ELISA ELISA Kit across 2 suppliers. ... Background: Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a rational target... read more ...
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ADP-Ribosylation Factor 4 (ARF4) ELISA KitsADP-Ribosylation Factor 4 (ARF4) ELISA Kits

ADP-ribosylation factor 4 , ADP-ribosylation factor 2 , ADP-ribosylation factor family-like protein ... ADP-Ribosylation Factor GTPase Activating Protein 3 ELISA Kits * ADP-Ribosylation Factor Guanine Nucleotide-Exchange Factor 1( ... Browse our ADP-Ribosylation Factor 4 (ARF4) ELISA Kits. Full name:. ADP-Ribosylation Factor 4 ELISA Kits (ARF4). On www. ... ADP-Ribosylation Factor GTPase Activating Protein 1 ELISA Kits * ADP-Ribosylation Factor GTPase Activating Protein 2 ELISA Kits ...
more infohttp://www.antibodies-online.com/egfr-signaling-pathway-pathway-26/arf4-elisa-kit-15744/

ADP-Ribosylation Factor 4 (ARF4) ELISA KitsADP-Ribosylation Factor 4 (ARF4) ELISA Kits

ADP-ribosylation factor 4 , ADP-ribosylation factor 2 , ADP-ribosylation factor family-like protein ... ADP-Ribosylation Factor GTPase Activating Protein 3 ELISA Kits * ADP-Ribosylation Factor Guanine Nucleotide-Exchange Factor 1( ... ADP-Ribosylation Factor 4 (ARF4) ELISA Kits. Bezeichnung:. ADP-Ribosylation Factor 4 ELISA Kits (ARF4). Auf www.antikoerper- ... ADP-Ribosylation Factor GTPase Activating Protein 1 ELISA Kits * ADP-Ribosylation Factor GTPase Activating Protein 2 ELISA Kits ...
more infohttp://www.antikoerper-online.de/egfr-signaling-pathway-pathway-26/arf4-elisa-kit-15744/

Arf6 - ADP-ribosylation factor 6 - Rattus norvegicus (Rat) - Arf6 gene & proteinArf6 - ADP-ribosylation factor 6 - Rattus norvegicus (Rat) - Arf6 gene & protein

ADP-ribosylation factor 6Add BLAST. 174. Amino acid modifications. Feature key. Position(s). DescriptionActions. Graphical view ... sp,P62332,ARF6_RAT ADP-ribosylation factor 6 OS=Rattus norvegicus GN=Arf6 PE=1 SV=2 ... Activation is generally mediated by guanine exchange factor (GEF), while inactivation through hydrolysis of bound GTP is ...
more infohttp://www.uniprot.org/uniprot/P62332

ARL6 ADP ribosylation factor like GTPase 6 [Homo sapiens (human)] - Gene - NCBIARL6 ADP ribosylation factor like GTPase 6 [Homo sapiens (human)] - Gene - NCBI

ARL6 ADP ribosylation factor like GTPase 6 [Homo sapiens] ARL6 ADP ribosylation factor like GTPase 6 [Homo sapiens]. Gene ID: ... ADP ribosylation factor like GTPase 6provided by HGNC. Primary source. HGNC:HGNC:13210 See related. Ensembl:ENSG00000113966 MIM ... ARL6 ADP ribosylation factor like GTPase 6 [ Homo sapiens (human) ] Gene ID: 84100, updated on 7-Dec-2018 ... The protein encoded by this gene belongs to the ARF-like (ADP ribosylation factor-like) sub-family of the ARF family of GTP- ...
more infohttps://www.ncbi.nlm.nih.gov/gene/84100

ARL5B - ADP-ribosylation factor-like 5B - Homo sapiens (Human) - ARL5B gene & proteinARL5B - ADP-ribosylation factor-like 5B - Homo sapiens (Human) - ARL5B gene & protein

ADP-ribosylation factor-like 5BImported. ,p>Information which has been imported from another database using automatic ... ADP-ribosylation factor-like protein 5BImported. Automatic assertion inferred from database entriesi ... tr,B0YIW9,B0YIW9_HUMAN ADP-ribosylation factor-like 5B OS=Homo sapiens OX=9606 GN=ARL5B PE=3 SV=1 ... ADP-ribosylation factor-like protein 5B. ). HUMAN. 179. UniRef100_Q96KC2. ADP-ribosylation factor-like protein 5B. MOUSE ...
more infohttps://www.uniprot.org/uniprot/B0YIW9

ADP-ribosylation factorADP-ribosylation factor

... stimulates cholera toxin ADP ribosylation. Mediates binding of non clathrin coated vesicles and AP1 (adaptor protein 1) of ... Arf6 - ADP ribosylation factor 6 (ARF6) is a member of the ADP ribosylation factor family of GTP binding proteins. ARF6 has a ... ADP ribosylation factor - Pfam box Symbol = Arf Name = width =250 caption =Membrane bound ADP ribosylation factor like protein ... ADP ribosylation factor 3, also known as ARF3, is a human gene.cite web , title = Entrez Gene: ARF3 ADP ribosylation factor 3, ...
more infohttp://en.academic.ru/dic.nsf/en_molecular_biology/178/ADP

Arl4c (ADP ribosylation factor like GTPase 4C) - Rat Genome DatabaseArl4c (ADP ribosylation factor like GTPase 4C) - Rat Genome Database

ADP-ribosylation factor-like 4C LOC367311 similar to ADP-ribosylation factor-like protein 4C (ADP-ribosylation factor-like 7) ... ADP-ribosylation factor-like 4C; ADP-ribosylation factor-like 4C, pseudogene 1; ADP-ribosylation factor-like protein 4C; Arl4c- ... ARL4C (ADP ribosylation factor like GTPase 4C). RGD. RGD. Mus musculus (house mouse):. Arl4c (ADP-ribosylation factor-like 4C) ... ADP-ribosylation factor like GTPase 4C Name changed. 629549. APPROVED. 2015-11-19. Arl4c ADP-ribosylation factor like GTPase 4C ...
more infohttps://rgd.mcw.edu/rgdweb/report/gene/main.html?id=1589685

arf3a, ADP-ribosylation factor 3a - Creative Biogenearf3a, ADP-ribosylation factor 3a - Creative Biogene

ARF3A; ADP-ribosylation factor 3a; zgc:92190; wu:fc66h11; ADP-ribosylation factor 3 ...
more infohttps://www.creative-biogene.com/symbolsearch_arf3a.html

ARF1 (ADP-ribosylation factor 1) - KOMP (Knockout Mouse Project)ARF1 (ADP-ribosylation factor 1) - KOMP (Knockout Mouse Project)

Vega: OTTMUSG6340 (Arf1, ADP-ribosylation factor 1)*CCDS: 24765, 24765.1*OMIM: ADP-RIBOSYLATION FACTOR 1; ARF1*Gene Ontology: ... ADP-ribosylation factor 1. Gene nomenclature, locus information, and GO, OMIM, and PMID associations are updated daily from MGI ...
more infohttps://www.komp.org/geneinfo.php?geneid=23594

ADP ribosylation factor - WikipediaADP ribosylation factor - Wikipedia

ADP ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily. ARF family ... The small ADP ribosylation factor (Arf) GTP-binding proteins are major regulators of vesicle biogenesis in intracellular ... Kahn RA, Kern FG, Clark J, Gelmann EP, Rulka C (1991). "Human ADP-ribosylation factors. A functionally conserved family of GTP- ... Amor JC, Harrison DH, Kahn RA, Ringe D (1994). "Structure of the human ADP-ribosylation factor 1 complexed with GDP". Nature. ...
more infohttps://en.wikipedia.org/wiki/ADP_ribosylation_factor
  • found ADP-ribosylation factor (ARF) 4 is one of the putative target genes of Mir-221, and the direct binding relationship was further confirmed by dual-luciferase reporter assay. (nih.gov)