MONOMERIC GTP-BINDING PROTEINS that were initially recognized as allosteric activators of the MONO(ADP-RIBOSE) TRANSFERASE of the CHOLERA TOXIN catalytic subunit. They are involved in vesicle trafficking and activation of PHOSPHOLIPASE D. This enzyme was formerly listed as EC 3.6.1.47
ADP-RIBOSYLATION FACTOR 1 is involved in regulating intracellular transport by modulating the interaction of coat proteins with organelle membranes in the early secretory pathway. It is a component of COAT PROTEIN COMPLEX I. This enzyme was formerly listed as EC 3.6.1.47.
A fungal metabolite which is a macrocyclic lactone exhibiting a wide range of antibiotic activity.
Vesicles formed when cell-membrane coated pits (COATED PITS, CELL-MEMBRANE) invaginate and pinch off. The outer surface of these vesicles are covered with a lattice-like network of coat proteins, such as CLATHRIN, coat protein complex proteins, or CAVEOLINS.
A 700-kDa cytosolic protein complex consisting of seven equimolar subunits (alpha, beta, beta', gamma, delta, epsilon and zeta). COATOMER PROTEIN and ADP-RIBOSYLATION FACTOR 1 are principle components of COAT PROTEIN COMPLEX I and are involved in vesicle transport between the ENDOPLASMIC RETICULUM and the GOLGI APPARATUS.
Protein factors that promote the exchange of GTP for GDP bound to GTP-BINDING PROTEINS.
Regulatory proteins that act as molecular switches. They control a wide range of biological processes including: receptor signaling, intracellular signal transduction pathways, and protein synthesis. Their activity is regulated by factors that control their ability to bind to and hydrolyze GTP to GDP. EC 3.6.1.-.
A protein complex comprised of COATOMER PROTEIN and ADP RIBOSYLATION FACTOR 1. It is involved in transport of vesicles between the ENDOPLASMIC RETICULUM and the GOLGI APPARATUS.
A stack of flattened vesicles that functions in posttranslational processing and sorting of proteins, receiving them from the rough ENDOPLASMIC RETICULUM and directing them to secretory vesicles, LYSOSOMES, or the CELL MEMBRANE. The movement of proteins takes place by transfer vesicles that bud off from the rough endoplasmic reticulum or Golgi apparatus and fuse with the Golgi, lysosomes or cell membrane. (From Glick, Glossary of Biochemistry and Molecular Biology, 1990)
Proteins that activate the GTPase of specific GTP-BINDING PROTEINS.
An ester formed between the aldehydic carbon of RIBOSE and the terminal phosphate of ADENOSINE DIPHOSPHATE. It is produced by the hydrolysis of nicotinamide-adenine dinucleotide (NAD) by a variety of enzymes, some of which transfer an ADP-ribosyl group to target proteins.
An enzyme found mostly in plant tissue. It hydrolyzes glycerophosphatidates with the formation of a phosphatidic acid and a nitrogenous base such as choline. This enzyme also catalyzes transphosphatidylation reactions. EC 3.1.4.4.
Guanosine 5'-(trihydrogen diphosphate), monoanhydride with phosphorothioic acid. A stable GTP analog which enjoys a variety of physiological actions such as stimulation of guanine nucleotide-binding proteins, phosphoinositide hydrolysis, cyclic AMP accumulation, and activation of specific proto-oncogenes.
Guanosine 5'-(tetrahydrogen triphosphate). A guanine nucleotide containing three phosphate groups esterified to the sugar moiety.
Esters formed between the aldehydic carbon of sugars and the terminal phosphate of adenosine diphosphate.
Cellular uptake of extracellular materials within membrane-limited vacuoles or microvesicles. ENDOSOMES play a central role in endocytosis.
The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
A pentose active in biological systems usually in its D-form.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
Enzymes that transfer the ADP-RIBOSE group of NAD or NADP to proteins or other small molecules. Transfer of ADP-ribose to water (i.e., hydrolysis) is catalyzed by the NADASES. The mono(ADP-ribose)transferases transfer a single ADP-ribose. POLY(ADP-RIBOSE) POLYMERASES transfer multiple units of ADP-ribose to protein targets, building POLY ADENOSINE DIPHOSPHATE RIBOSE in linear or branched chains.
Transport proteins that carry specific substances in the blood or across cell membranes.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
An ENTEROTOXIN from VIBRIO CHOLERAE. It consists of two major protomers, the heavy (H) or A subunit and the B protomer which consists of 5 light (L) or B subunits. The catalytic A subunit is proteolytically cleaved into fragments A1 and A2. The A1 fragment is a MONO(ADP-RIBOSE) TRANSFERASE. The B protomer binds cholera toxin to intestinal epithelial cells, and facilitates the uptake of the A1 fragment. The A1 catalyzed transfer of ADP-RIBOSE to the alpha subunits of heterotrimeric G PROTEINS activates the production of CYCLIC AMP. Increased levels of cyclic AMP are thought to modulate release of fluid and electrolytes from intestinal crypt cells.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
One of the virulence factors produced by BORDETELLA PERTUSSIS. It is a multimeric protein composed of five subunits S1 - S5. S1 contains mono ADPribose transferase activity.
A set of BACTERIAL ADHESINS and TOXINS, BIOLOGICAL produced by BORDETELLA organisms that determine the pathogenesis of BORDETELLA INFECTIONS, such as WHOOPING COUGH. They include filamentous hemagglutinin; FIMBRIAE PROTEINS; pertactin; PERTUSSIS TOXIN; ADENYLATE CYCLASE TOXIN; dermonecrotic toxin; tracheal cytotoxin; Bordetella LIPOPOLYSACCHARIDES; and tracheal colonization factor.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Adenosine 5'-(trihydrogen diphosphate). An adenine nucleotide containing two phosphate groups esterified to the sugar moiety at the 5'-position.
Enzymes that catalyze the transfer of multiple ADP-RIBOSE groups from nicotinamide-adenine dinucleotide (NAD) onto protein targets, thus building up a linear or branched homopolymer of repeating ADP-ribose units i.e., POLY ADENOSINE DIPHOSPHATE RIBOSE.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Toxic proteins produced from the species CLOSTRIDIUM BOTULINUM. The toxins are synthesized as a single peptide chain which is processed into a mature protein consisting of a heavy chain and light chain joined via a disulfide bond. The botulinum toxin light chain is a zinc-dependent protease which is released from the heavy chain upon ENDOCYTOSIS into PRESYNAPTIC NERVE ENDINGS. Once inside the cell the botulinum toxin light chain cleaves specific SNARE proteins which are essential for secretion of ACETYLCHOLINE by SYNAPTIC VESICLES. This inhibition of acetylcholine release results in muscular PARALYSIS.
A coenzyme composed of ribosylnicotinamide 5'-diphosphate coupled to adenosine 5'-phosphate by pyrophosphate linkage. It is found widely in nature and is involved in numerous enzymatic reactions in which it serves as an electron carrier by being alternately oxidized (NAD+) and reduced (NADH). (Dorland, 27th ed)
An enzyme of the lyase class that catalyzes the formation of CYCLIC AMP and pyrophosphate from ATP. EC 4.6.1.1.
One of the virulence factors produced by virulent BORDETELLA organisms. It is a bifunctional protein with both ADENYLYL CYCLASES and hemolysin components.
A polynucleotide formed from the ADP-RIBOSE moiety of nicotinamide-adenine dinucleotide (NAD) by POLY(ADP-RIBOSE) POLYMERASES.
A phosphoinositide present in all eukaryotic cells, particularly in the plasma membrane. It is the major substrate for receptor-stimulated phosphoinositidase C, with the consequent formation of inositol 1,4,5-triphosphate and diacylglycerol, and probably also for receptor-stimulated inositol phospholipid 3-kinase. (Kendrew, The Encyclopedia of Molecular Biology, 1994)
Toxic substances formed in or elaborated by bacteria; they are usually proteins with high molecular weight and antigenicity; some are used as antibiotics and some to skin test for the presence of or susceptibility to certain diseases.
The rate dynamics in chemical or physical systems.
A class of proteins involved in the transport of molecules via TRANSPORT VESICLES. They perform functions such as binding to the cell membrane, capturing cargo molecules and promoting the assembly of CLATHRIN. The majority of adaptor proteins exist as multi-subunit complexes, however monomeric varieties have also been found.
A RHO GTP-BINDING PROTEIN involved in regulating signal transduction pathways that control assembly of focal adhesions and actin stress fibers. This enzyme was formerly listed as EC 3.6.1.47.
Proteins prepared by recombinant DNA technology.
One of the early purine analogs showing antineoplastic activity. It functions as an antimetabolite and is easily incorporated into ribonucleic acids.
Thin structures that encapsulate subcellular structures or ORGANELLES in EUKARYOTIC CELLS. They include a variety of membranes associated with the CELL NUCLEUS; the MITOCHONDRIA; the GOLGI APPARATUS; the ENDOPLASMIC RETICULUM; LYSOSOMES; PLASTIDS; and VACUOLES.
Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
Established cell cultures that have the potential to propagate indefinitely.
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
A group of telomere associated proteins that interact with TRF1 PROTEIN, contain ANKYRIN REPEATS and have poly(ADP-ribose) polymerase activity.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). The species is a favorite for cytogenetic studies because of its small chromosome number. The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells.
A class of nucleotide translocases found abundantly in mitochondria that function as integral components of the inner mitochondrial membrane. They facilitate the exchange of ADP and ATP between the cytosol and the mitochondria, thereby linking the subcellular compartments of ATP production to those of ATP utilization.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.

Identification of a new Pyk2 target protein with Arf-GAP activity. (1/429)

Protein tyrosine kinase Pyk2 is activated by a variety of G-protein-coupled receptors and by extracellular signals that elevate intracellular Ca2+ concentration. We have identified a new Pyk2 binding protein designated Pap. Pap is a multidomain protein composed of an N-terminal alpha-helical region with a coiled-coil motif, followed by a pleckstrin homology domain, an Arf-GAP domain, an ankyrin homology region, a proline-rich region, and a C-terminal SH3 domain. We demonstrate that Pap forms a stable complex with Pyk2 and that activation of Pyk2 leads to tyrosine phosphorylation of Pap in living cells. Immunofluorescence experiments demonstrate that Pap is localized in the Golgi apparatus and at the plasma membrane, where it is colocalized with Pyk2. In addition, in vitro recombinant Pap exhibits strong GTPase-activating protein (GAP) activity towards the small GTPases Arf1 and Arf5 and weak activity towards Arf6. Addition of recombinant Pap protein to Golgi preparations prevented Arf-dependent generation of post-Golgi vesicles in vitro. Moreover, overexpression of Pap in cultured cells reduced the constitutive secretion of a marker protein. We propose that Pap functions as a GAP for Arf and that Pyk2 may be involved in regulation of vesicular transport through its interaction with Pap.  (+info)

GCS1, an Arf guanosine triphosphatase-activating protein in Saccharomyces cerevisiae, is required for normal actin cytoskeletal organization in vivo and stimulates actin polymerization in vitro. (2/429)

Recent cloning of a rat brain phosphatidylinositol 3,4, 5-trisphosphate binding protein, centaurin alpha, identified a novel gene family based on homology to an amino-terminal zinc-binding domain. In Saccharomyces cerevisiae, the protein with the highest homology to centaurin alpha is Gcs1p, the product of the GCS1 gene. GCS1 was originally identified as a gene conditionally required for the reentry of cells into the cell cycle after stationary phase growth. Gcs1p was previously characterized as a guanosine triphosphatase-activating protein for the small guanosine triphosphatase Arf1, and gcs1 mutants displayed vesicle-trafficking defects. Here, we have shown that similar to centaurin alpha, recombinant Gcs1p bound phosphoinositide-based affinity resins with high affinity and specificity. A novel GCS1 disruption strain (gcs1Delta) exhibited morphological defects, as well as mislocalization of cortical actin patches. gcs1Delta was hypersensitive to the actin monomer-sequestering drug, latrunculin-B. Synthetic lethality was observed between null alleles of GCS1 and SLA2, the gene encoding a protein involved in stabilization of the actin cytoskeleton. In addition, synthetic growth defects were observed between null alleles of GCS1 and SAC6, the gene encoding the yeast fimbrin homologue. Recombinant Gcs1p bound to actin filaments, stimulated actin polymerization, and inhibited actin depolymerization in vitro. These data provide in vivo and in vitro evidence that Gcs1p interacts directly with the actin cytoskeleton in S. cerevisiae.  (+info)

EFA6, a sec7 domain-containing exchange factor for ARF6, coordinates membrane recycling and actin cytoskeleton organization. (3/429)

We have identified a human cDNA encoding a novel protein, exchange factor for ARF6 (EFA6), which contains Sec7 and pleckstrin homology domains. EFA6 promotes efficient guanine nucleotide exchange on ARF6 and is distinct from the ARNO family of ARF1 exchange factors. The protein localizes to a dense matrix on the cytoplasmic face of plasma membrane invaginations, induced on its expression. We show that EFA6 regulates endosomal membrane recycling and promotes the redistribution of transferrin receptors to the cell surface. Furthermore, expression of EFA6 induces actin-based membrane ruffles that are inhibited by co-expression of dominant-inhibitory mutant forms of ARF6 or Rac1. Our results demonstrate that by catalyzing nucleotide exchange on ARF6 at the plasma membrane and by regulating Rac1 activation, EFA6 coordinates endocytosis with cytoskeletal rearrangements.  (+info)

Characterization of the regulation of phospholipase D activity in the detergent-insoluble fraction of HL60 cells by protein kinase C and small G-proteins. (4/429)

Phospholipase D (PLD) activity has been shown to be GTP-dependent both in vivo and in vitro. One protein that confers GTP sensitivity to PLD activity in vitro is the low-molecular-mass G-protein ADP-ribosylation factor (Arf). However, members of the Rho family and protein kinase C (PKC) have also been reported to activate PLD in various cell systems. We have characterized the stimulation of PLD in HL60 cell membranes by these proteins. The results demonstrate that a considerable proportion of HL60 PLD activity is located in a detergent-insoluble fraction of the cell membrane that is unlikely to be a caveolae-like domain, but is probably cytoskeletal. This PLD activity required the presence of Arf1, a Rho-family member and PKC for efficient catalysis of the lipid substrate, suggesting that the activity represents PLD1. We show that recombinant human PLD1b is regulated in a similar manner to HL60-membrane PLD, and that PKCalpha and PKCdelta are equally effective PLD activators. Therefore maximum PLD activity requires Arf, a Rho-family member and PKC, emphasizing the high degree of regulation of this enzyme.  (+info)

Structural and functional analysis of the ARF1-ARFGAP complex reveals a role for coatomer in GTP hydrolysis. (5/429)

The crystal structure of the complex of ARF1 GTPase bound to GDP and the catalytic domain of ARF GTPase-activating protein (ARFGAP) has been determined at 1.95 A resolution. The ARFGAP molecule binds to switch 2 and helix alpha3 to orient ARF1 residues for catalysis, but it supplies neither arginine nor other amino acid side chains to the GTPase active site. In the complex, the effector-binding region appears to be unobstructed, suggesting that ARFGAP could stimulate GTP hydrolysis while ARF1 maintains an interaction with its effector, the coatomer complex of COPI-coated vesicles. Biochemical experiments show that coatomer directly participates in the GTPase reaction, accelerating GTP hydrolysis a further 1000-fold in an ARFGAP-dependent manner. Thus, a tripartite complex controls the GTP hydrolysis reaction triggering disassembly of COPI vesicle coats.  (+info)

Expression and distribution of adenosine diphosphate-ribosylation factors in the rat kidney. (6/429)

BACKGROUND: Adenosine diphosphate (ADP)-ribosylation factors (ARFs) are small guanosine triphosphatases involved in membrane traffic regulation. Aiming to explore the possible involvement of ARF1 and ARF6 in the reabsorptive properties of the nephron, we evaluated their distribution along the different renal epithelial segments. METHODS: ARFs were detected by immunofluorescence and immunogold cytochemistry on renal sections, using specific anti-ARF antibodies. RESULTS: ARF1 was detected in proximal and distal tubules, thick ascending limbs of Henle's loops, and cortical and medullary collecting ducts. By immunofluorescence, labeling was mostly localized to the cell cytoplasm, particularly in Golgi areas. By electron microscopy, the Golgi apparatus and the endosomal compartment of proximal and distal tubular cells were labeled. ARF6 immunofluorescence was observed in brush border membranes and the cytoplasm of proximal convoluted tubular cells, whereas it was restricted to the apical border of proximal straight tubules. ARF6 immunogold labeling was detected over microvilli and endocytic compartments of proximal tubular cells. CONCLUSIONS: This study demonstrates the following: (a) the heterogeneous distributions of ARF1 and ARF6 along the nephron, (b) the existence of cytosolic and membrane-bound forms for both ARFs, and (c) their association with microvilli and endocytic compartments, suggesting an active participation in renal reabsorption.  (+info)

Purification and cloning of a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP-ribosylation factors. (7/429)

Activation of ADP-ribosylation factors (ARFs), approximately 20-kDa guanine nucleotide-binding proteins that play an important role in intracellular vesicular trafficking, depends on guanine nucleotide-exchange proteins (GEPs), which accelerate replacement of bound GDP with GTP. Two major families of ARF GEPs are known: approximately 200-kDa molecules that are inhibited by brefeldin A (BFA), a fungal metabolite that blocks protein secretion and causes apparent disintegration of Golgi structure, and approximately 50-kDa GEPs that are insensitive to BFA. We describe here two human brain cDNAs that encode BFA-inhibited GEPs. One is a approximately 209-kDa protein 99.5% identical in deduced amino acid sequence (1, 849 residues) to a BFA-inhibited ARF GEP (p200) from bovine brain. The other smaller protein, which is approximately 74% identical (1, 785 amino acids), represents a previously unknown gene. We propose that the former, p200, be named BIG1 for (brefeldin A-inhibited GEP1) and the second, which encodes a approximately 202-kDa protein, BIG2. A protein containing sequences found in BIG2 had been purified earlier from bovine brain. Human tissues contained a 7.5-kilobase BIG1 mRNA and a 9.4-kilobase BIG2 transcript. The BIG1 and BIG2 genes were localized, respectively, to chromosomes 8 and 20. BIG2, synthesized as a His6 fusion protein in Sf9 cells, accelerated guanosine 5'-3-O-(thio)triphosphate binding by recombinant ARF1, ARF5, and ARF6. It activated native ARF (mixture of ARF1 and ARF3) more effectively than it did any of the nonmyristoylated recombinant ARFs. BIG2 activity was inhibited by BFA in a concentration-dependent manner but not by B17, a structural analog without effects on Golgi function. Although several clones for approximately 50-kDa BFA-insensitive ARF GEPs are known, these new clones for the approximately 200-kDa BIG1 and BIG2 should facilitate characterization of this rather different family of proteins as well as the elucidation of mechanisms of regulation of BFA-sensitive ARF function in Golgi transport.  (+info)

Structural elements of ADP-ribosylation factor 1 required for functional interaction with cytohesin-1. (8/429)

ADP-ribosylation factor 1 (ARF1) is a 20-kDa guanine nucleotide-binding protein involved in vesicular trafficking. Conversion of inactive ARF-GDP to active ARF-GTP is catalyzed by guanine nucleotide exchange proteins such as cytohesin-1. Cytohesin-1 and its Sec7 domain (C-1Sec7) exhibit guanine nucleotide exchange protein activity with ARF1 but not ARF-like protein 1 (ARL1), which is 57% identical in amino acid sequence. With chimeric proteins composed of ARF1 (F) and ARL1 (L) sequences we identified three structural elements responsible for this specificity. Cytohesin-1 increased [35S]guanosine 5'-(gamma-thio)triphosphate binding to L28/F (first 28 residues of L, remainder F) and to a much lesser extent F139/L, and mut13F139/L (F139/L with random sequence in the first 13 positions) but not Delta13ARF1 that lacks the first 13 amino acids; therefore, a nonspecific ARF N terminus was required for cytohesin-1 action. The N terminus was not, however, required for that of C-1Sec7. Both C-1Sec7 and cytohesin-1 effectively released guanosine 5'-(gamma-thio)triphosphate from ARF1, but only C-1Sec7 displaced the nonhydrolyzable GTP analog bound to mut13F139/L, again indicating that structure in addition to the Sec7 domain is involved in cytohesin-1 interaction. Some element(s) of the C-terminal region is also involved, because replacement of the last 42 amino acids with ARL sequence in F139L decreased markedly the interaction with cytohesin-1. Participation of both termini is consistent with the crystallographic structure of ARF in which the two terminal alpha-helices are in close proximity. ARF1 residues 28-50 are also important in the interaction with cytohesin-1; replacement of Lys-38 with Gln, the corresponding residue in ARL1, abolished the ability to serve as substrate for cytohesin-1 or C-1Sec7. These studies have defined multiple structural elements in ARF1, including switch 1 and the N and C termini, that participate in functional interactions with cytohesin-1 (or its catalytic domain C-1Sec7), which were not apparent from crystallographic analysis.  (+info)

ADP-ribosylation factor (ARF) proteins and inhibitory peptides derived from ARFs have demonstrated activities in a number of in vitro assays that measure ER-to-Golgi and intra-Golgi transport and endosome fusion. To better understand the roles of ARF proteins in vivo, stable cell lines were obtained from normal rat kidney (NRK) cells transfected with either wild-type or a dominant activating allele ([Q71L]) of the human ARF1 gene under the control of the interferon-inducible mouse Mx1 promoter. Upon addition of interferon, expression of ARF1 proteins increased with a half-time of 7-8 h, as determined by immunoblot analysis. Induction of mutant ARF1, but not wild-type ARF1, led to an inhibition of protein secretion with kinetics similar to that observed for induction of protein expression. Examination of the Golgi apparatus and the ER by indirect immunofluorescence or transmission electron microscopy revealed that expression of low levels of mutant ARF1 protein correlated with a dramatic increase ...
Promotes guanine-nucleotide exchange on ARF1 and ARF6. Promotes the activation of ARF factors through replacement of GDP with GTP. Play a role in the epithelial polarization (By similarity).
ADP-ribosylation factors (ARFs) are approximately 20-kDa guanine nucleotide-binding proteins recognized as critical components in intracellular vesicular transport and phospholipase D activation. Both guanine nucleotide-exchange proteins and GTPase-activating proteins (GAPs) for ARFs have been cloned recently. A zinc finger motif near the amino terminus of the ARF1 GAP was required for stimulation of GTP hydrolysis. ARD1 is an ARF family member that differs from other ARFs by the presence of a 46-kDa amino-terminal extension. We had reported that the ARF domain of ARD1 binds specifically GDP and GTP and that the amino-terminal extension acts as a GAP for the ARF domain of ARD1 but not for ARF proteins. The GAP domain of ARD1, synthesized in Escherichia coli, stimulated hydrolysis of GTP bound to the ARF domain of ARD1. Using ARD1 truncations, it appears that amino acids 101-190 are critical for GAP activity, whereas residues 190-333 are involved in physical interaction between the two domains of ...
Ver más] The small GTP-binding protein ADP-ribosylation factor 1 (ARF1) is an essential component of the molecular machinery that catalyzes the formation of membranebound transport intermediates. By using an in vitro assay that reproduces recruitment of cytosolic proteins onto purified, high salt-washed Golgi membranes, we have analyzed the role of cAMP-dependent protein kinase A (PKA) on ARF1 incorporation. Addition to this assay of either pure catalytic subunits of PKA (C-PKA) or cAMP increased ARF1 binding. By contrast, ARF1 association was inhibited following C-PKA inactivation with either PKA inhibitory peptide or RIIa as well as after cytosol depletion of C-PKA. C-PKA also stimulated recruitment and activation of a recombinant form of human ARF1 in the absence of additional cytosolic components. The binding step could be dissociated from the activation reaction and found to be independent of guanine nucleotides and saturable. This step was stimulated by C-PKA in an ATP-dependent manner. ...
TY - JOUR. T1 - ADP ribosylation factor 6 regulates neuronal migration in the developing cerebral cortex through FIP3/arfophilin-1-dependent endosomal trafficking of N-cadherin. AU - Hara, Yoshinobu. AU - Fukaya, Masahiro. AU - Hayashi, Kanehiro. AU - Kawauchi, Takeshi. AU - Nakajima, Kazunori. AU - Sakagami, Hiroyuki. PY - 2016. Y1 - 2016. N2 - During neural development, endosomal trafficking controls cell shape and motility through the polarized transport of membrane proteins related to cellcell and cellextracellular matrix interactions. ADP ribosylation factor 6 (Arf6) is a critical small GTPase that regulates membrane trafficking between the plasma membrane and endosomes. We herein demonstrated that the knockdown of endogenous Arf6 in mouse cerebral cortices led to impaired neuronal migration in the intermediate zone and cytoplasmic retention of N-cadherin and syntaxin12 in migrating neurons. Rescue experiments with separation-of-function Arf6 mutants identified Rab11 familyinteracting ...
D. Jones, B. Bax, S. Cockcroft; ADP-ribosylation factor GTPases in signal transduction and membrane traffic: independent functions?. Biochem Soc Trans 1 August 1999; 27 (4): 642-647. doi: https://doi.org/10.1042/bst0270642. Download citation file:. ...
Top performende anti-Ratte (Rattus) ADP-Ribosylation Factor 1 Antikörper für Immunofluorescence (Paraffin-embedded Sections) (IF (p)) vergleichen & kaufen.
Cell migration is an orchestrated and highly coordinated multi-step process that is central to the development and maintenance of multicellular organisms. Dysregulated migration however, is associated with pathological states such as tumor formation and metastasis; thus a clear understanding of the molecular mechanisms that drive this process is critical to the development of counteracting therapeutics. Cell migration and adhesion-dependent cell spreading share a number of features. For example, both processes rely on the activation of mechanisms for the coordinated spatial and temporal assembly/disassembly of focal adhesions, as well as mechanisms controlling actin rearrangements and directed vesicular trafficking. Actin remodeling and vesicular trafficking events are in turn, implicated functions of a variety of small GTPases of the Ras superfamily, which include the Rho and Arf subfamilies. Thus towards efforts of further characterizing the molecular pathways that drive cell spreading, I pursued aims
COPⅠ囊泡:最初研究者利用三磷酸鳥苷(GTP)衍生物GTPγS(一種富含高爾基體膜的細胞質與抗水解的GTP衍生物)共培養時,發現高爾基體池之間存在一種囊泡轉運結構[9](後來在真核細胞中也證實此結構的存在[10])。除了脂質成分外,參與此囊泡形成的成份還有7種外被體蛋白(即外被體α、β、β′、γ、δ、ε、ζ)。這些外被體蛋白相互作用形成的復合物就是COPⅠ囊泡[11][12]。亞單位α、β′、ε在結構上與網格蛋白及COPⅡ囊泡的外層組分具有較高的一致性,形成復合物的內層組分稱為B亞復合物(主要負責與靶蛋白結合),而亞單位β、γ、δ、ζ 與網格蛋白及COPⅡ囊泡的內層組分相似,形成復合物的內層組分稱為F亞復合物,該亞復合物主要負責與靶蛋白結合,並且直接與COPⅠ囊泡形成的招募者ADP核糖基化因子(英语:ADP ribosylation factor)(ADP ribosylation ...
Angiogenesis: Growth of new blood vessels by sprouting from existing ones. Anoxia: a condition characterized by an absence of oxygen supply to an organ or a tissue Apoptosis: Form of cell death, also known as programmed cell death, in which a suicide program is activated within the cell, leading to fragmentation of the DNA, shrinkage of the cytoplasm, membrane changes and cell death without lysis or damage to neighboring cells. It is a normal phenomenon, occurring frequently in a multicellular organism. ARF: ADP Ribosylation Factor (ARF) is a member of the GTP-binding proteins responsible for regulating both COPI coat assembly and clathrin coat assembly at Golgi membranes. ATM: a protein that regulates several cellular responses to DNA breaks. C. elegans: Caenorhabditis elegans is a nematode (unsegmented) worm with very simple anatomy. Chaperone (molecular chaperone): Protein that helps other proteins avoid misfolding pathways that produce inactive or aggregated polypeptides. Drosophila: ...
The protein encoded by this gene catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. The activity of the encoded enzyme is enhanced by phosphatidylinositol 4,5-bisphosphate and ADP-ribosylation factor-1. This protein localizes to the peripheral membrane and may be involved in cytoskeletal organization, cell cycle control, transcriptional regulation, and/or regulated secretion. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011 ...
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy ...
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Complete information for ARF6 gene (Protein Coding), ADP Ribosylation Factor 6, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
ARF1 - ARF1 (untagged)-Human ADP-ribosylation factor 1 (ARF1), transcript variant 4 available for purchase from OriGene - Your Gene Company.
Complete information for ARL6IP6 gene (Protein Coding), ADP Ribosylation Factor Like GTPase 6 Interacting Protein 6, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
ARFGAP3 antibody, C-term (ADP-ribosylation factor GTPase activating protein 3) for WB. Anti-ARFGAP3 pAb (GTX89988) is tested in Mouse samples. 100% Ab-Assurance.
ADP-ribosylation factor, GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the ...
ARFIP2 antibody [N1C2] (ADP-ribosylation factor interacting protein 2) for IHC-P, WB. Anti-ARFIP2 pAb (GTX104241) is tested in Human samples. 100% Ab-Assurance.
Brefeldin A-inhibited guanine nucleotide-exchange protein 3 (BIG3) has been identified recently as a novel regulator of estrogen signalling in breast cancer cells. Despite being a potential target for new breast cancer treatment, its amino acid sequence suggests no association with any well-characterized protein family and provides little clues as to its molecular function. In this paper, we predicted the structure, function and interactions of BIG3 using a range of bioinformatic tools. Homology search results showed that BIG3 had distinct features from its paralogues, BIG1 and BIG2, with a unique region between the two shared domains, Sec7 and DUF1981. Although BIG3 contains Sec7 domain, the lack of the conserved motif and the critical glutamate residue suggested no potential guaninyl-exchange factor (GEF) activity. Fold recognition tools predicted BIG3 to adopt an α-helical repeat structure similar to that of the armadillo (ARM) family. Using state-of-the-art methods, we predicted interaction sites
This gene encodes a member of the GIT protein family, which interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. GIT proteins traffic between cytoplasmic complexes, focal adhesions, and the cell periphery, and interact with Pak interacting exchange factor beta (PIX) to form large oligomeric complexes that transiently recruit other proteins. GIT proteins regulate cytoskeletal dynamics and participate in receptor internalization and membrane trafficking. This gene has been shown to repress lamellipodial extension and focal adhesion turnover, and is thought to regulate cell motility. This gene undergoes extensive alternative splicing to generate multiple isoforms, but the full-length nature of some of these variants has not been determined. The various isoforms have functional differences, with respect to ARF GAP activity and to G protein-coupled receptor kinase 2 binding. [provided by RefSeq, Sep 2008 ...
Coat protein complex I (COPI) vesicles are involved in transport processes within the early secretory pathway (Bethune et al., 2006). For their biogenesis, the small GTPase ADP ribosylation factor 1 (Arf1) in its GDP-bound form is recruited to the Golgi membrane by dimeric transmembrane proteins of the p24 family (Gommel et al., 2001) or by interaction with membrin (Honda et al., 2005). The membrane-associated Arf guanine nucleotide exchange factor GBF1 catalyzes exchange of the bound GDP to GTP (Zhao et al., 2006). Arf1-GTP dissociates from the p24 proteins and is inserted into the Golgi membrane (Franco et al., 1996; Antonny et al., 1997) as a dimer (Beck et al., 2008) to recruit the heptameric protein complex coatomer (Palmer et al., 1993). Coatomer polymerization leads to the formation of a COPI-coated vesicle (Bremser et al., 1999; Reinhard et al., 1999). Arf GTPase-activating proteins (GAPs) catalyze hydrolysis of the GTP bound to Arf1 followed by dissociation of the coat (Tanigawa et al., ...
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Arl1 (ARF like protein1) is a poorly understood member of ARF family small GTPases. This thesis presents an original characterization of Arl1 and its effectors. Arl1 was localized to the tans Golgi under EM. Over expression of guanine nucleotide mutants of Arl1 dramatically affects the structure and function of Golgi apparatus. Arl1-GTP was found to interact with GRIP domain of Golgins (Golgin-97, Golgin-245, GCC1 and KIAA0336). The interaction was dependent on the conserved amino acids on both switch II region of Arl1 and the GRIP domain. Collectively, the research presented in this thesis reveals Arl1 is a new regulator of Golgi structure and function and one mechanism of Arl1a??s function is that it recruits and regulates its effectors a?? GRIP domain Golgins to Golgi ...
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the GIT protein family, which interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. GIT proteins traffic between cytoplasmic complexes, focal adhesions, and the cell periphery, and interact with Pak interacting exchange factor beta (PIX) to form large oligomeric complexes that transiently recruit other proteins. GIT proteins regulate cytoskeletal dynamics and participate in receptor internalization and membrane trafficking. This gene has been shown to repress lamellipodial extension and focal adhesion turnover, and is thought to regulate cell motility. This gene undergoes extensive alternative splicing to generate multiple isoforms, but the full-length nature of some of these variants has not been determined. The various isoforms have functional differences, with respect to ARF GAP activity ...
In eukaryotes, calcium signalling has been linked to hydrolysis of the phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P(2)). The final enzyme in the synthesis of this phosphoinositide, a Type I phosphatidylinositol 4-phosphate 5-kinase (PIP5K), is activated by the small G protein ADP-ribosylation factor 1 (ARF1). In mammals, the ARF-PIP5K pathway is a key regulator of cell motility, secretion and cell signalling. We report the characterisation of a unique, putative bifunctional PIP5K in the human malaria parasite Plasmodium falciparum. The protein comprises a C-terminal, functional PIP5K domain with catalytic specificity for phosphatidylinositol 4-phosphate. The recombinant enzyme is activated by ARF1 but not phosphatidic acid. The protein also incorporates an unusual N-terminal domain with potential helix-loop-helix EF-hand-like motifs that is a member of the neuronal calcium sensor family (NCS). Intriguingly, NCS-1 has been shown to stimulate phosphatidylinositol 4-phosphate synthesis by ...
Two widely expressed mammalian phosphatidylcholine (PC)-specific phospholipases D (PLD), PLD1 and PLD2, have been identified. Recombinantly expressed PLD2 has high basal activity and is insensitive to GTP-binding protein activators of PLD1 [Colley, W. C., et al. (1997) Curr. Biol. 7, 191-201]. To investigate the regulation of PLD2 we isolated PLD2, from mouse brain by immunoaffinity chromatography. The native and recombinant proteins have indistinguishable properties: PLD2 is potently activated by phosphoinositides with a vicinal 4,5-phosphate pair but is not stimulated by guanosine 5-O-(3-thio triphosphate)-activated ADP-ribosylation factor-1, Rho family GTP-binding proteins, or protein kinases C-alpha, or -beta1 ...
Other than a role for Ca2+ store depletion, the molecular mechanisms that regulate antigen-stimulated Ca2+ influx into mast cells are not well-understood. The observation that CT dramatically enhances 45Ca2+ influx into RBL-2H3 cells suggests that this reagent might be a useful tool to study the Ca2+ entry pathway (Narasimhan et al., 1988). That CT amplifies both antigen-evoked ICRAC and 45Ca2+ influx to a similar extent bolsters the idea that CRAC channels are a major pathway for FcεRI-mediated Ca2+ uptake into RBL-2H3 mast cells (Zhang and McCloskey, 1995).. Two hypotheses to explain the effect of CT on 45Ca2+ influx are immediately testable by patch clamping. First, it is possible that CT activates Cl− or K+ channels, and thereby increases the electrical force propelling Ca2+ entry. This indirect mechanism cannot explain the enhancement of Ca2+ influx currents that we observed, because voltage-clamp measurements eliminate any difference in membrane potential between control and CT-treated ...
GTP-binding protein involved in protein trafficking that regulates endocytic recycling and cytoskeleton remodeling. Required for normal completion of mitotic cytokinesis. May also modulate vesicle budding and uncoating within the Golgi apparatus. Involved in the regulation of dendritic spine development, contributing to the regulation of dendritic branching and filopodia extension (PubMed:16672654). Involved in epithelial polarization (By similarity).
ADP ribosylation factor 1 (Arf1). Arl1 subfamily. Arl1 (Arf-like 1) localizes to the Golgi complex, where it is believed to recruit effector proteins to the trans-Golgi network. Like most members of the Arf family, Arl1 is myristoylated at its N-terminal helix and mutation of the myristoylation site disrupts Golgi targeting. In humans, the Golgi-localized proteins golgin-97 and golgin-245 have been identified as Arl1 effectors. Golgins are large coiled-coil proteins found in the Golgi, and these golgins contain a C-terminal GRIP domain, which is the site of Arl1 binding. Additional Arl1 effectors include the GARP (Golgi-associated retrograde protein)/VFT (Vps53) vesicle-tethering complex and Arfaptin 2. Arl1 is not required for exocytosis, but appears necessary for trafficking from the endosomes to the Golgi. In Drosophila zygotes, mutation of Arl1 is lethal, and in the host-bloodstream form of Trypanosoma brucei, Arl1 is essential for viability. ...
BFA induces the ADP-ribosylation of BARS-50 and GAPDH in permeabilized cells. (A) RBL cells were permeabilized with 3 U/ml SLO and exposed to 10 μg/ml BFA
Stimulates the exchange of guanyl nucleotides associated with the GTPase ARF. Under normal cellular physiological conditions, the concentration of GTP is higher than that of GDP, favoring the replacement of GDP by GTP in association with the GTPase ...
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Arfgef1 (untagged) - Mouse ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited) (Arfgef1), (10ug), 10 µg.
We recently reported that brefeldin A-inhibited guanine nucleotide-exchange proteins 3 (BIG3) binds Prohibitin 2 (PHB2) in cytoplasm, thereby leading to a reduction of function of the PHB2 growth suppressor in the nuclei of breasts tumor cells. PHB2 nuclear transfer may offer restorative strategies for managing Elizabeth2/Emergency room signs in breasts tumor cells. Introduction Prohibitin 1 and 2 (PHB and buy 808118-40-3 PHB2) proteins are highly conserved in eukaryotic cells and exhibit diverse subcellular localization with different functions [1C3]. These molecules are primarily observed in inner mitochondrial membranes via their buy 808118-40-3 N-terminal transmembrane domain but are also present in several other localizations such as the cytosol, endoplasmic reticulum, nucleus, and plasma membrane [1]. Both proteins form hetero-oligomeric ring structures in the inner mitochondrial membrane and function as chaperones buy 808118-40-3 that maintain mitochondrial integrity and stabilize ...
FUNCTION: Guanine nucleotide-exchange factor (GEF) required for the formation or budding of transport vesicles from the ER. This function involves the cytoplasmic domain of the protein, which is thought to interact with the small GTP-binding protein SAR1. Required for autophagy. MISCELLANEOUS: In the process of transport, SEC12 itself may migrate to the Golgi apparatus and function in subsequent transport events. MISCELLANEOUS: Present with 6160 molecules/cell in log phase SD medium ...
The small GTP-binding proteins of the ARF family play a central role in membrane dynamics and protein transport in eukaryotic cells. The GEFs that catalyze GDP/GTP exchange on ARF are of critical importance to ARF function, as they determine when and where ARF proteins will be activated within the cell (reviewed by Jackson and Casanova, 2000). We present here an in vivo characterization of the Gea1p and Gea2p ARF GEFs in the secretory pathway of S. cerevisiae. The Gea1p and Gea2p proteins are members of a subfamily of ARF GEFs with members in plants and animals, as well as in yeast ( Claude et al., 1999; Steinmann et al., 1999). This subfamily is distinct from that of Sec7p and its mammalian orthologues BIG1 and BIG2 ( Mansour et al., 1999; Morinaga et al., 1997; Togawa et al., 1999).. The Gea1p and Gea2p proteins are functionally redundant, but at least one is necessary for viability in yeast. We have generated three temperature-sensitive gea mutants and have examined their phenotypes. The ...
Looking for online definition of ADP-ribosylation factor-like tumour-suppressor protein 1 in the Medical Dictionary? ADP-ribosylation factor-like tumour-suppressor protein 1 explanation free. What is ADP-ribosylation factor-like tumour-suppressor protein 1? Meaning of ADP-ribosylation factor-like tumour-suppressor protein 1 medical term. What does ADP-ribosylation factor-like tumour-suppressor protein 1 mean?
Arginine adenosine-5′-diphosphoribosylation (ADP-ribosylation) is an enzyme-catalyzed potentially reversible posttranslational changes where the ADP-ribose moiety is transferred from NAD+ towards the guanidino CHR2797 moiety of arginine. proteins with binding companions e.g. toxin-catalyzed ADP-ribosylation of actin at R177 blocks actin polymerization sterically. In case there is the nucleotide-gated P2X7 ion route ADP-ribosylation at R125 near the ligand-binding site causes route Rabbit Polyclonal to SF3B3. gating. Arginine-specific ADP-ribosyltransferases (ARTs) bring a quality R-S-EXE theme that distinguishes these enzymes from structurally related enzymes which catalyze ADP-ribosylation of additional amino acid part chains DNA or little substances. Arginine-specific ADP-ribosylation could be inhibited by little molecule arginine analogues such as for example CHR2797 agmatine or meta-iodobenzylguanidine (MIBG) which themselves can serve as focuses on for arginine-specific ARTs. ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
ADP-Ribosylation Factor 1 is involved in regulating intracellular transport by modulating the interaction of Coat Proteins with Organelle Membranes in the early Secretory Pathway. It is a component of Coat Protein Complex I. This enzyme was formerly listed as EC 3.6.1.47 ...
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
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Microinjection of the slowly hydrolyzable GTP analogue GTP(gamma)S or the ectopic expression of a GTP restricted mutant of the small GTPase arf1 (arf1[Q71L]) leads to the rapid accumulation of COPI coated vesicles and buds in living cells. This effect is blocked at 15 degrees C and by microinjection of antibodies against (beta)-COP. Anterograde and retrograde membrane protein transport markers, which have been previously shown to be incorporated into COPI vesicles between the endoplasmic reticulum and Golgi complex, are depleted from the GTP(gamma)S or arf1[Q71L] induced COPI coated vesicles and buds. In contrast, in control cells 30 to 60% of the COPI carriers co-localize with these markers. These in vivo data corroborate recent in vitro work, suggesting that GTP(gamma)S and arf1[Q71L] interfere with the sorting of membrane proteins into Golgi derived COPI vesicles, and provide the first in vivo evidence for a role of GTP hydrolysis by arf1 in the sorting of cargo into COPI coated vesicles and ...
ADP ribosylation factor 6 (Arf6) is a small GTPase that regulates dendritic differentiation possibly through the organization of actin cytoskeleton and membrane traffic. Here, we characterized IQ-ArfGEF/BRAG1, a guanine nucleotide exchange factor (GEF) for Arf6, in the mouse brain. In vivo Arf pull …
CIN85 is a multidomain adaptor protein involved in Cbl-mediated down-regulation of epidermal growth factor (EGF) receptors. CIN85 src homology 3 domains specifically bind to a proline-arginine (PxxxPR) motif in Cbl, and this association seems to be important for EGF receptor endocytosis. Here, we report identification of novel CIN85 effectors, all containing one or more PxxxPR motifs, that are indispensable for their mutual interactions. These effectors include phosphatidyl-inositol phosphatases SHIP-1 and synaptojanin 2B1, Arf GTPase-activating proteins ASAP1 and ARAP3, adaptor proteins Hip1R and STAP1, and a Rho exchange factor, p115Rho GEF. Acting as a molecular scaffold, CIN85 clusters its effectors and recruits them to high-molecular-weight complexes in cytosolic extracts of cells. Further characterization of CIN85 binding to ASAP1 revealed that formation of the complex is independent on cell stimulation. Overexpression of ASAP1 increased EGF receptor recycling, whereas ASAP1 containing ...
Small GTPases largely control membrane traffic, which is essential for the survival of all eukaryotes. Among the small GTP-binding proteins, ARF1 (ADP-ribosylation factor 1) and SAR1 (Secretion-Associated RAS super family 1) are commonly conserved among all eukaryotes with respect to both their functional and sequential characteristics. The ARF1 and SAR1 GTP-binding proteins are involved in the formation and budding of vesicles throughout plant endomembrane systems. ARF1 has been shown to play a critical role in COPI (Coat Protein Complex I)-mediated retrograde trafficking in eukaryotic systems, whereas SAR1 GTPases are involved in intracellular COPII-mediated protein trafficking from the ER to the Golgi apparatus. This review offers a summary of vesicular trafficking with an emphasis on the ARF1 and SAR1 expression patterns at early growth stages and in the de-etiolation process.
Hong Xu wrote: , , Dear Colleagues: , , Our group is using the Clontech s HeLa cDNA library in a yeast , two-hybrid screening with the bait fused to the GAL4AD and the yeast , strain CG 1945. Many of the clones sequenced show one of the following , sequences, including ITBA2 (whatever it is, since almost no references , can be found in Medline), ribosomal s20, transketolase, Ring12 (probably , a proteasome subunit), ADP ribosylation factor, etc., with those listed , above showing up most frequently. We would like to know whether or not , these factors have also been found in somebody else two-hybrid , screenings using the same Clontech HeLa library. , , You are welcome to post your answer to our question in this newsgroup. , Or you can call me at 973-781-7987. , , Thank you very much. , , Richard Dear Richard, try this URL and you find an overview of the most common trash found in two hybrid screens: http://www.fccc.edu/research/labs/golemis/main_false.html Hope this helps, Ricky ...
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Frank S, Upender S, Hansen SH, Casanova JE (1998). "ARNO is a guanine nucleotide exchange factor for ADP-ribosylation factor 6 ... ADP-ribosylation factor 1 is a protein that in humans is encoded by the ARF1 gene. ADP-ribosylation factor 1 (ARF1) is a member ... 1991). "ADP-ribosylation factor is a subunit of the coat of Golgi-derived COP-coated vesicles: a novel role for a GTP-binding ... "Entrez Gene: ARF1 ADP-ribosylation factor 1". Mitchell R, Robertson DN, Holland PJ, Collins D, Lutz EM, Johnson MS (September ...
ADP-ribosylation factor-like) family of proteins, which are structurally related to ADP-ribosylation factors (ARFs). ARFs, ... ADP-ribosylation factor-like protein 1 is a protein that in humans is encoded by the ARL1 gene. The protein encoded by this ... "Entrez Gene: ARL1 ADP-ribosylation factor-like 1". CS1 maint: discouraged parameter (link) Lu L, Hong W (September 2003). " ... Van Valkenburgh H, Shern JF, Sharer JD, Zhu X, Kahn RA (June 2001). "ADP-ribosylation factors (ARFs) and ARF-like 1 (ARL1) have ...
ADP-ribosylation factor-related protein 1 is a protein that in humans is encoded by the ARFRP1 gene. The protein encoded by ... "Entrez Gene: ARFRP1 ADP-ribosylation factor related protein 1". Schürmann A, Schmidt M, Asmus M, Bayer S, Fliegert F, Koling S ... It is related to the ADP-ribosylation factor (ARF) and ARF-like (ARL) genes. The gene is located in a gene cluster that ... 1999). "The ADP-ribosylation factor (ARF)-related GTPase ARF-related protein binds to the ARF-specific guanine nucleotide ...
ADP-ribosylation factor-like protein 6-interacting protein 1 is a protein that in humans is encoded by the ARL6IP1 gene. GRCh38 ... "Entrez Gene: ARL6IP1 ADP-ribosylation factor-like 6 interacting protein 1". Human ARL6IP1 genome location and ARL6IP1 gene ... "A novel ADP-ribosylation like factor (ARL-6), interacts with the protein-conducting channel SEC61beta subunit". FEBS Lett. 459 ... ADP-ribosylation-like factor-6 interacting protein (ARL6)". Genomics. 68 (3): 351-4. doi:10.1006/geno.2000.6278. PMID 10995579 ...
"Entrez Gene: ARFIP1 ADP-ribosylation factor interacting protein 1 (arfaptin 1)". Tsai SC, Adamik R, Hong JX, et al. (1998). " ... a putative cytosolic target protein of ADP-ribosylation factor, is recruited to Golgi membranes". J Biol Chem. 272 (9): 5421-9 ... Arfaptin-1 is a protein that in humans is encoded by the ARFIP1 gene. ARFIP1 has been shown to interact with ARF3. GRCh38: ... 537 (1-3): 91-5. doi:10.1016/S0014-5793(03)00098-X. PMID 12606037. S2CID 44551128. Ota T, Suzuki Y, Nishikawa T, et al. (2004 ...
Frank S, Upender S, Hansen SH, Casanova JE (1998). "ARNO is a guanine nucleotide exchange factor for ADP-ribosylation factor 6 ... Lee SY, Pohajdak B (2000). "N-terminal targeting of guanine nucleotide exchange factors (GEF) for ADP ribosylation factors (ARF ... Claing A, Chen W, Miller WE, Vitale N, Moss J, Premont RT, Lefkowitz RJ (2001). "beta-Arrestin-mediated ADP-ribosylation factor ... "beta-Arrestin-mediated ADP-ribosylation factor 6 activation and beta 2-adrenergic receptor endocytosis". J. Biol. Chem. 276 (45 ...
This protein is also a member of the ADP ribosylation factor family of guanine nucleotide-binding family of proteins. Its ... Vitale N, Horiba K, Ferrans VJ, Moss J, Vaughan M (Jul 1998). "Localization of ADP-ribosylation factor domain protein 1 (ARD1) ... Vitale N, Moss J, Vaughan M (Oct 1997). "Characterization of a GDP dissociation inhibitory region of ADP-ribosylation factor ... Vitale N, Moss J, Vaughan M (Jan 1998). "Molecular characterization of the GTPase-activating domain of ADP-ribosylation factor ...
"Activation of phospholipase D1 by direct interaction with ADP-ribosylation factor 1 and RalA". FEBS Letters. 430 (3): 231-5. ... "Activation of phospholipase D1 by direct interaction with ADP-ribosylation factor 1 and RalA". FEBS Letters. 430 (3): 231-5. ... a pleckstrin homology domain containing guanine nucleotide exchange factor for Ral". The Journal of Biological Chemistry. 275 ( ... 4 (1): 73-8. doi:10.1038/ncb720. PMID 11744922. S2CID 9528945. Clough RR, Sidhu RS, Bhullar RP (Aug 2002). "Calmodulin binds ...
December 1995). "Human ADP-ribosylation factor-activated phosphatidylcholine-specific phospholipase D defines a new and highly ... July 1998). "Activation of phospholipase D1 by direct interaction with ADP-ribosylation factor 1 and RalA". FEBS Letters. 430 ( ... July 1998). "Activation of phospholipase D1 by direct interaction with ADP-ribosylation factor 1 and RalA". FEBS Letters. 430 ( ... Activation of the purified enzymes by phosphatidylinositol 4,5-bisphosphate, ADP-ribosylation factor, and Rho family monomeric ...
1 (3): 287-92. doi:10.1093/embo-reports/kvd058. PMC 1083732. PMID 11256614. Deloukas P, Matthews LH, Ashurst J, et al. (2002 ... 2000). "Specific functional interaction of human cytohesin-1 and ADP-ribosylation factor domain protein (ARD1)". J. Biol. Chem ...
"Entrez Gene: ARFIP2 ADP-ribosylation factor interacting protein 2 (arfaptin 2)". D'Souza-Schorey C, Boshans RL, McDonough M, ... Shin OH, Exton JH (August 2001). "Differential binding of arfaptin 2/POR1 to ADP-ribosylation factors and Rac1". Biochemical ... Shin OH, Exton JH (August 2001). "Differential binding of arfaptin 2/POR1 to ADP-ribosylation factors and Rac1". Biochemical ... a putative cytosolic target protein of ADP-ribosylation factor, is recruited to Golgi membranes". The Journal of Biological ...
... binds to 2 molecules of ADP-ribosylation factor 1 (Arf1) as shown in this figure. A hinge region of exomer is thought to ...
ADP-ribosylation factor 5 is a protein that in humans is encoded by the ARF5 gene. ADP-ribosylation factor 5 (ARF5) is a member ... "Entrez Gene: ARF5 ADP-ribosylation factor 5". Kanoh, H; Williger B T; Exton J H (February 1997). "Arfaptin 1, a putative ... Shin OH, Couvillon AD, Exton JH (2001). "Arfophilin is a common target of both class II and class III ADP-ribosylation factors ... Shin, O H; Exton J H (August 2001). "Differential binding of arfaptin 2/POR1 to ADP-ribosylation factors and Rac1". Biochem. ...
2002). "Recruitment to Golgi membranes of ADP-ribosylation factor 1 is mediated by the cytoplasmic domain of p23". EMBO J. 20 ( ... 200 (1-2): 149-56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149. Gommel D, Orci L, Emig EM, et al. (1999). "p24 and p23, the ... 138 (1-2): 171-4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298. Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). " ... 1 (1): 139-53. doi:10.1016/S1534-5807(01)00004-1. PMID 11703931. Gommel DU, Memon AR, Heiss A, et al. ( ...
ADP-ribosylation factor GTPase-activating protein 1 is an enzyme that in humans is encoded by the ARFGAP1 gene. Two transcript ... which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). The encoded protein ...
ADP-ribosylation factor-like protein 3 is a protein that in humans is encoded by the ARL3 gene. ADP-ribosylation factor-like 3 ... "Entrez Gene: ARL3 ADP-ribosylation factor-like 3". Stelzl U, Worm U, Lalowski M, Haenig C, Brembeck FH, Goehler H, Stroedicke M ... Van Valkenburgh H, Shern JF, Sharer JD, Zhu X, Kahn RA (June 2001). "ADP-ribosylation factors (ARFs) and ARF-like 1 (ARL1) have ... Kim HS (1998). "Assignment of the human ADP-ribosylation factor-like 3 (ARL3) gene to chromosome 10 band q23.3 by radiation ...
Van Valkenburgh H, Shern JF, Sharer JD, Zhu X, Kahn RA (June 2001). "ADP-ribosylation factors (ARFs) and ARF-like 1 (ARL1) have ... Van Valkenburgh H, Shern JF, Sharer JD, Zhu X, Kahn RA (June 2001). "ADP-ribosylation factors (ARFs) and ARF-like 1 (ARL1) have ... 534 (1-3): 26-32. doi:10.1016/S0014-5793(02)03766-3. PMID 12527357. S2CID 22603052. Stelzl U, Worm U, Lalowski M, Haenig C, ... 534 (1-3): 26-32. doi:10.1016/S0014-5793(02)03766-3. PMID 12527357. S2CID 22603052. Rual JF, Venkatesan K, Hao T, Hirozane- ...
Van Valkenburgh, H; Shern J F; Sharer J D; Zhu X; Kahn R A (June 2001). "ADP-ribosylation factors (ARFs) and ARF-like 1 (ARL1) ... 2001). "ADP-ribosylation factors (ARFs) and ARF-like 1 (ARL1) have both specific and shared effectors: characterizing ARL1- ... 36 (1): 40-45. doi:10.1038/ng1285. PMID 14702039. Anderson NL, Polanski M, Pieper R, et al. (2004). "The human plasma proteome ... 6 (1): 37-44. doi:10.1093/dnares/6.1.37. PMID 10231028. Eichmuller S, Usener D, Dummer R, et al. (2001). "Serological detection ...
Pacheco-Rodriguez G, Meacci E, Vitale N, Moss J, Vaughan M (1998). "Guanine nucleotide exchange on ADP-ribosylation factors ... "The ADP-ribosylation factor (ARF)-related GTPase ARF-related protein binds to the ARF-specific guanine nucleotide exchange ... "The ADP-ribosylation factor (ARF)-related GTPase ARF-related protein binds to the ARF-specific guanine nucleotide exchange ... Sec7 domain and its interaction with the GTPase ADP ribosylation factor 1". Proc. Natl. Acad. Sci. U.S.A. 95 (14): 7909-14. doi ...
1998). "Nerve growth factor- and epidermal growth factor-stimulated translocation of the ADP-ribosylation factor-exchange ... 1998). "ARNO3, a Sec7-domain guanine nucleotide exchange factor for ADP ribosylation factor 1, is involved in the control of ... and it may have a physiological role in regulating ADP-ribosylation factor protein 6 (ARF) functions, in addition to acting on ... guanine nucleotide-exchange proteins for ADP-ribosylation factors". J. Biol. Chem. 275 (5): 3221-30. doi:10.1074/jbc.275.5.3221 ...
... guanine nucleotide-exchange proteins for ADP-ribosylation factors". J. Biol. Chem. 275 (5): 3221-30. doi:10.1074/jbc.275.5.3221 ... 2008). "GEP100 links epidermal growth factor receptor signalling to Arf6 activation to induce breast cancer invasion". Nat. ... Venkateswarlu K (2003). "Interaction protein for cytohesin exchange factors 1 (IPCEF1) binds cytohesin 2 and modifies its ... 10 (1): 85-92. doi:10.1038/ncb1672. PMID 18084281. Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and ...
ADP-ribosylation factor 6 (ARF6) is a member of the ADP ribosylation factor family of GTP-binding proteins. ARF6 has a variety ... Frank S, Upender S, Hansen SH, Casanova JE (1998). "ARNO is a guanine nucleotide exchange factor for ADP-ribosylation factor 6 ... "ADP-ribosylation factor 6 as a target of guanine nucleotide exchange factor GRP1". J. Biol. Chem. 274 (38): 27099-104. doi: ... "Entrez Gene: ARF6 ADP-ribosylation factor 6". Claing A, Chen W, Miller WE, Vitale N, Moss J, Premont RT, Lefkowitz RJ (November ...
ADP-ribosylation factor 3 is a protein that in humans is encoded by the ARF3 gene. ADP-ribosylation factor 3 (ARF3) is a member ... "Entrez Gene: ARF3 ADP-ribosylation factor 3". Kanoh H, Williger BT, Exton JH (February 1997). "Arfaptin 1, a putative cytosolic ... Lee CM, Haun RS, Tsai SC, Moss J, Vaughan M (1992). "Characterization of the human gene encoding ADP-ribosylation factor 1, a ... Boman AL, Zhang Cj, Zhu X, Kahn RA (April 2000). "A family of ADP-ribosylation factor effectors that can alter membrane ...
April 2005). "Exocytosis of CTLA-4 is dependent on phospholipase D and ADP ribosylation factor-1 and stimulated during ... July 2020). "Blood factors transfer beneficial effects of exercise on neurogenesis and cognition to the aged brain". Science. ... Lugli EB, Pouliot M, Portela M, Loomis MR, Raper J (November 2004). "Characterization of primate trypanosome lytic factors". ... 53 (1): 21-38. PMID 11939716. Jaworek J, Bonio J, Leja-Szpa A, Nawrot K, Tomaszewska MR, Stachura J, et al. (March 2002). " ...
"Entrez Gene: ARFGEF1 ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited)". Padilla PI, Chang MJ ... ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. The protein encoded by this gene ... "Isolation of a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP ribosylation factor (ARF) 1 and ARF3 that ... "Purification and cloning of a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP-ribosylation factors". The ...
... and 3 ADP-ribosylation factors with adaptor protein complexes 1 and 3". Biochemistry. 41 (14): 4669-77. doi:10.1021/bi016064j. ... 271 (1): 9-17. doi:10.1006/viro.2000.0277. PMID 10814565. Crump CM, Xiang Y, Thomas L, Gu F, Austin C, Tooze SA, Thomas G (May ... AP-3 complex subunit delta-1 is a protein that in humans is encoded by the AP3D1 gene. AP3D1 is a subunit of the AP3 adaptor- ... 21 (1): 111-22. doi:10.1016/S0896-6273(00)80519-X. PMID 9697856. S2CID 17237941. Rehling P, Darsow T, Katzmann DJ, Emr SD (2000 ...
... and 3 ADP-ribosylation factors with adaptor protein complexes 1 and 3". Biochemistry. 41 (14): 4669-77. doi:10.1021/bi016064j. ... Eiraku M, Hirata Y, Takeshima H, Hirano T, Kengaku M (Jul 2002). "Delta/notch-like epidermal growth factor (EGF)-related ... AP-1 complex subunit gamma-1 is a protein that in humans is encoded by the AP1G1 gene. Adaptins are important components of ... 22 (1): 78-88. doi:10.1093/emboj/cdg015. PMC 140067. PMID 12505986. Horikawa HP, Kneussel M, El Far O, Betz H (Nov 2002). " ...
In vitro it shows strong GTPase-activating protein (GAP) activity towards the small GTPases ADP-ribosylation factor (ARF) 1 and ... 2001). "Pag3/Papα/Kiaa0400, a Gtpase-Activating Protein for Adp-Ribosylation Factor (Arf), Regulates Arf6 in Fcγ Receptor- ... 2000). "A New Paxillin-binding Protein, PAG3/Papα/KIAA0400, Bearing an ADP-Ribosylation Factor GTPase-activating Protein ... Kim H, Lee Y (2002). "Interaction of poly(A) polymerase with the 25-kDa subunit of cleavage factor I". Biochem. Biophys. Res. ...
2000). "Type I phosphatidylinositol 4-phosphate 5-kinase directly interacts with ADP-ribosylation factor 1 and is responsible ... Phosphatidylinositol-4-phosphate 5-kinase type-1 alpha is an enzyme that in humans is encoded by the PIP5K1A gene. GRCh38: ... 2006). "The DNA sequence and biological annotation of human chromosome 1". Nature. 441 (7091): 315-21. Bibcode:2006Natur.441.. ...
2001). "Identification and characterization of RA-GEF-2, a Rap guanine nucleotide exchange factor that serves as a downstream ... 2003). "Characterisation of PDZ-GEFs, a family of guanine nucleotide exchange factors specific for Rap1 and Rap2". Biochim. ... Mitochondrial GTPase 1 is an enzyme that in humans is encoded by the MTG1 gene. GRCh38: Ensembl release 89: ENSG00000148824 - ... 36 (1): 40-5. doi:10.1038/ng1285. PMID 14702039. Deloukas P, Earthrowl ME, Grafham DV, et al. (2004). "The DNA sequence and ...
ARL6IP1: encoding protein ADP-ribosylation factor-like protein 6-interacting protein 1 ... PMFBP1: encoding protein Polyamine-modulated factor 1-binding protein 1. *POLR3K: encoding enzyme DNA-directed RNA polymerase ... ISBN 978-1-136-84407-2.. *^ a b Genome Decoration Page, NCBI. Ideogram data for Homo sapience (850 bphs, Assembly GRCh38.p3). ... SHCBP1: encoding protein SHC SH2 domain-binding protein 1. *SLZ1: encoding protein SLX1 structure-specific endonuclease subunit ...
... and ADP-ribosylation. This affects their function of gene regulation. In general, genes that are active have less bound histone ... SBF is a transcription factor that is activated in late G1 phase, when it dissociates from its repressor Whi5. This occurs when ... and ADP-ribosylation. The core of the histones H2A and H2B can also be modified. Combinations of modifications are thought to ... The serotonylation potentiates the binding of the general transcription factor TFIID to the TATA box.[49] ...
In addition, the inactivation of Rho protein by ADP-ribosylation in Rac1 microinjection reduced the formation of actin stress ... This led to the conclusion that CNK1 couples specific Rho exchange factors to the JNK MAP kinase pathway, providing specificity ... The inhibition of Rho by C3 transferase ribosylation resulted in an inhibition of focal adhesion and stress fibre assembly, but ... Indeed, Rho-dependent response is stimulated by the action of growth factors on Rac protein.[8] ...
ADP-ribosyl)ation directs recruitment and activation of an ATP-dependent chromatin remodeler". Proc. Natl. Acad. Sci. U.S.A. ... "Epigenetic factors in cancer risk: effect of chemical carcinogens on global DNA methylation pattern in human TK6 cells". PLoS ... ADP)-ribose polymerase) and its product poly(ADP)-ribose (PAR) accumulate at sites of DNA damage as part of a repair process.[ ... Malanga M, Althaus FR (2005). "The role of poly(ADP-ribose) in the DNA damage signaling network". Biochem Cell Biol. 83 (3): ...
... and the A1 subunit is freed to bind with a human partner protein called ADP-ribosylation factor 6 (Arf6).[29] Binding exposes ... The A1 portion of the A subunit is an enzyme that ADP-ribosylates G proteins, while the A2 chain fits into the central pore of ... "Sources of Infection & Risk Factors". Centers for Disease Control and Prevention. November 7, 2014. Archived from the original ... Risk factors for the disease include poor sanitation, not enough clean drinking water, and poverty.[2] There are concerns that ...
ADP-ribosylation, and ubiquitination have also been reported.[3][22] These combinations of different covalent modifications on ... and the general transcription factors TFIIE and TFIIF.[4] Other substrates include the transcription factors Sp1, KLF5, FOXO1, ... MORF (MOZ-related factor) exhibits very close homology to MOZ throughout its entire length.[4] It contains an N-terminal ... TAFII250 is one of the TBP-associated factor subunits of TFIID, and it shares a Gly-X-Gly pattern with Gcn5 that is important ...
... to arginine or other guanidine compounds by utilizing ADP-ribosylation factors (ARFs). In the absence of arginine or simple ... Locht, C; Antoine, R (1995). "A proposed mechanism of ADP-ribosylation catalyzed by the pertussis toxin S1 subunit". Biochimie ... the A1 domain unfolds and is redirected back to the cytosol where it refolds and catalyzes ADP-ribosylation of certain G ... This is accomplished through fusion of epidermal growth factor(EGF) with SubAB's A subunit. Cancer cells that express receptors ...
Proline hydroxylation is also a vital component of hypoxia response via hypoxia inducible factors. In some cases, proline may ... ADP-ribosylation. *Deamination. *Oxidative deamination to aldehyde. *O-glycosylation. *Imine formation. *Glycation ... 978-1-119-45166-2. .. *^ Co and Post-Translational Modifications of Therapeutic Antibodies and Proteins, John Wiley & Sons, ... ISBN 1-57259-153-6.[page needed] *^ Zurlo, Giada; Guo, Jianping; Takada, Mamoru; Wei, Wenyi; Zhang, Qing (December 2016). "New ...
Corda D, Di Girolamo M «Functional aspects of protein mono-ADP-ribosylation». EMBO J., 22, 9, 2003, pàg. 1953-8. DOI: 10.1093/ ... The isolation and identification of the anti-black tongue factor» (PDF). J. Biol. Chem., 123, 1, 1938, pàg. 137-49. ... La poli-ADP-ribosilació té lloc gràcies a l'acció de les poli(ADP-ribosa) polimerases.[53][50] L'estructura poli(ADP-ribosa) ... que s'anomena poli-ADP-ribosilació.[50] La mono-ADP-ribosliació es va identificar per primera vegada en el mecanisme d'un ...
ARL6IP1: encoding protein ADP-ribosylation factor-like protein 6-interacting protein 1 ... PMFBP1: encoding protein Polyamine-modulated factor 1-binding protein 1. *POLR3K: encoding enzyme DNA-directed RNA polymerase ... 978-1-136-84407-2. .. *^ a b Genome Decoration Page, NCBI. Ideogram data for Homo sapience (850 bphs, Assembly GRCh38.p3). Last ... 978-1-4673-1921-8. .. *^ Genome Decoration Page, NCBI. Ideogram data for Homo sapience (850 bphs, Assembly GRCh38.p3). Last ...
nucleotide addition such as ADP-ribosylation. *phosphate ester (O-linked) or phosphoramidate (N-linked) formation * ... beta-Lysine addition on a conserved lysine of the elongation factor P (EFP) in most bacteria.[9] EFP is an homolog to eIF5A ( ... Modifications of translation factorsEdit. *diphthamide formation (on a histidine found in eEF2) ... 1989). "Murine elongation factor 1 alpha (EF-1 alpha) is posttranslationally modified by novel amide-linked ethanolamine- ...
"Functional aspects of protein mono-ADP-ribosylation". EMBO J. 22 (9): 1953-8. PMC 156081. PMID 12727863. doi:10.1093/emboj/ ... Chamaron cofermento ao factor non identificado responsable deste efecto. A través dunha longa e dificultosa purificación a ... A ADP-ribosilación implica quer a adición dun só grupo ADP-ribosa (mono-ADP-ribosilación) quer a transferencia de ADP-ribosa ás ... ADP-ribosil)ación lévana a cabo as poli(ADP-ribosa) polimerases.[53][56] A estrutura da poli-(ADP-ribosa) está implicada na ...
... dependent ADP-ribosylation of elongation factor 2, thereby inhibiting protein synthesis in eukaryotic cells. Fragment B binds ... C. diphtheriae produces diphtheria toxin which alters protein function in the host by inactivating the elongation factor EF-2. ...
It acts similarly to the cholera toxin by raising cAMP levels through ADP-ribosylation of the alpha-subunit of a Gs protein ... The heat-labile enterotoxin is inactivated at high temperatures.[1][2]. ... factor. *Clumping factor A. *Fibronectin binding protein A. Mycotoxins. *Aflatoxin. *Amatoxin (alpha-amanitin, beta-amanitin, ...
D-glucose + ATP → D-glucose-6-phosphate + ADP. ΔG° = −16.7 kJ/mol (° indicates measurement at standard condition). Researcher D ... identify dynamic changes in the phosphorylation status of more than 6000 sites after stimulation with epidermal growth factor.[ ... is synthesized in the mitochondrion by addition of a third phosphate group to ADP in a process referred to as oxidative ... 1 (3): 327-41. doi:10.1586/14789450.1.3.327. PMID 15966829.. *^ Chen H, Kovar J, Sissons S, Cox K, Matter W, Chadwell F, Luan P ...
ADP-ribosyl)ation directs recruitment and activation of an ATP-dependent chromatin remodeler". Proc. Natl. Acad. Sci. U.S.A. ... Such effects on cellular and physiological phenotypic traits may result from external or environmental factors, or be part of ... ADP)-ribose polymerase) and its product poly(ADP)-ribose (PAR) accumulate at sites of DNA damage as part of a repair process.[ ... The yeast prion PSI is generated by a conformational change of a translation termination factor, which is then inherited by ...
regulation of protein ADP-ribosylation. • positive regulation of protein complex assembly. • positive regulation of protein ... Joseph AM, Kumar M, Mitra D (2005). "Nef: "necessary and enforcing factor" in HIV infection". Curr. HIV Res. 3 (1): 87-94. doi: ... positive regulation of tumor necrosis factor production. • CD8-positive, alpha-beta T cell differentiation involved in immune ... positive regulation of tumor necrosis factor biosynthetic process. • positive regulation of glycolytic process. • astrocyte ...
Shin OH, Exton JH (Aug 2001). "Differential binding of arfaptin 2/POR1 to ADP-ribosylation factors and Rac1". Biochemical and ... hepatocyte growth factor receptor signaling pathway. • regulation of stress fiber assembly. • positive regulation of protein ... vascular endothelial growth factor receptor signaling pathway. • substrate adhesion-dependent cell spreading. • cell ... of rac1 specifies interaction with a subset of guanine nucleotide exchange factors". The Journal of Biological Chemistry. 276 ( ...
Molecular characterization of the GTPase-activating domain of ADP-ribosylation factor domain protein 1 (ARD1)". J. Biol. Chem. ... Najpoznatiji članovi su Ras GTPaze, i iz tog razloga se ova familija enzima ponekad naziva Ras superfamilijom GTPaza.[1][2][3][ ...
ADP-ribosylation involves either the addition of a single ADP-ribose moiety, in mono-ADP-ribosylation, or the transferral of ... In bacteriology, NAD, sometimes referred to factor V, is used a supplement to culture media for some fastidious bacteria.[90] ... ADP-ribose to proteins in long branched chains, which is called poly(ADP-ribosyl)ation.[54] Mono-ADP-ribosylation was first ... ADP-ribosyl)ation is carried out by the poly(ADP-ribose) polymerases.[54][57] The poly(ADP-ribose) structure is involved in the ...
CTA1 is then free to bind with a human partner protein called ADP-ribosylation factor 6 (Arf6); binding to Arf6 drives a change ... The CTA1 fragment catalyses ADP-ribosylation of the Gs alpha subunit (Gαs) proteins using NAD. The ADP-ribosylation causes the ... The A1 portion of the chain (CTA1) is a globular enzyme payload that ADP-ribosylates G proteins, while the A2 chain (CTA2) ... produced by Bordetella pertussis acts in a similar manner with the exception that it ADP-ribosylates the Gαi subunit, rendering ...
ADP ribosylation factor)(ADP ribosylation factors)結合,從而參與啟動囊泡的逆向轉運[11][12][13]。同時又發現COPγ、COPζ各有2種亞型,即γ1、γ2、ζ1、ζ2[14],這些亞型間的交叉組合 ... 循環細胞膜囊泡(Tissue factor-carrying microvesicles、TF-MV):是一種細胞在激活、受損或凋亡時從細胞膜上釋放的脂
bHLH transcription factor binding. • NAD+ binding. • identical protein binding. • NAD+ ADP-ribosyltransferase activity. • ... protein ADP-ribosylation. • leptin-mediated signaling pathway. • negative regulation of apoptotic process. • negative ... cellular response to leukemia inhibitory factor. • positive regulation of angiogenesis. • transforming growth factor beta ... They add the acetyl group from the protein to the ADP-ribose component of NAD+ to form O-acetyl-ADP-ribose. The HDAC activity ...
A protein coat assembles and disassembles due to an ADP ribosylation factor (ARF) protein. ... 7 (1): 1535750. doi:10.1080/20013078.2018.1535750. PMC 6322352. PMID 30637094.. *^ a b c d e van der Pol, Edwin; Böing, Anita N ... 14 (1): 48-56. doi:10.1111/jth.13190. PMID 26564379.. *^ Mateescu B, Kowal EJ, van Balkom BW, et al. (2017). "Obstacles and ... carrying and transmitting virulence factors into host cells and modulating host defense and response.[14] ...
"Function of BRCA1 in the DNA damage response is mediated by ADP-ribosylation". Cancer Cell. 23 (5): 693-704. doi:10.1016/j.ccr. ... Kleiman FE, Manley JL (Sep 1999). "Functional interaction of BRCA1-associated BARD1 with polyadenylation factor CstF-50". ... Kleiman FE, Manley JL (Sep 1999). "Functional interaction of BRCA1-associated BARD1 with polyadenylation factor CstF-50". ... ADP-ribose) (PAR), which targets the BRCA1/BARD1 heterodimer to damaged DNA sites.[13] Double stranded breaks (DSB) in DNA ...
ADP-ribosyl)ation". Nat. Cell Biol. 9 (10): 1175-83. doi:10.1038/ncb1638. PMID 17891139.. ... "The zinc finger cluster domain of RanBP2 is a specific docking site for the nuclear export factor, exportin-1". J. Biol. Chem. ... "Entrez Gene: XPO1 exportin 1 (CRM1 homolog, yeast)".. *^ a b Tickenbrock L, Cramer J, Vetter IR, Muller O (August 2002). "The ... Exportin 1 (XPO1), also known as chromosomal maintenance 1 (CRM1), is an eukaryotic protein that mediates the nuclear export of ...
Zhao L, Helms JB, Brunner J, Wieland FT (1999). "GTP-dependent binding of ADP-ribosylation factor to coatomer in close ... Koatomerna podjedinica gama-2 je protein koji je kod ljudi kodiran COPG2 genom.[1][2][3] ... Biol. 3 (1): 89. PMC 1847948. PMID 17353931. doi:10.1038/msb4100134. CS1 održavanje: Eksplicitna upotreba et al. (link) ... Genet. 36 (1): 40-5. PMID 14702039. doi:10.1038/ng1285. CS1 održavanje: Eksplicitna upotreba et al. (link) ...
ARFGEF2: ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) ... ISBN 978-1-136-84407-2.. *^ a b Genome Decoration Page, NCBI. Ideogram data for Homo sapience (850 bphs, Assembly GRCh38.p3). ... 1 (3): 225-229. doi:10.1089/gte.1997.1.225. PMID 10464650.. *^ Genome Decoration Page, NCBI. Ideogram data for Homo sapience ( ... BPIFB1: encoding protein BPI fold containing family B, member 1. *BPIFB4: encoding protein BPI fold containing family B, member ...
... belongs to an ADP-ribosylation factor GTPase-activating (ARF-GAP) protein family involved in membrane traffic and actin ... phosphoinositide-dependent ADP-ribosylation factor GTPase-activating protein that affects actin cytoskeleton". The Journal of ... 128 (1): 9-13. doi:10.1016/j.cell.2006.12.018. PMID 17218247. S2CID 18872015. White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, ... Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 is an enzyme that in humans is encoded by the AGAP1 gene. ...
ADP-ribosylation factor-like) family of proteins, which are structurally related to ADP-ribosylation factors (ARFs). ARFs, ... ADP ribosylation factor like GTPase 1provided by HGNC. Primary source. HGNC:HGNC:692 See related. Ensembl:ENSG00000120805 MIM: ... ARL1 ADP ribosylation factor like GTPase 1 [Homo sapiens] ARL1 ADP ribosylation factor like GTPase 1 [Homo sapiens]. Gene ID: ... ARL1 ADP ribosylation factor like GTPase 1 [ Homo sapiens (human) ] Gene ID: 400, updated on 17-May-2020 ...
Q7KQL3 - Plasmodium falciparum 1 * Q3THZ2 - Mus musculus no matching PDB entries * P10947 - Homo sapiens no matching PDB ...
GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase ...
Compare ADP ribosylation factor interacting protein 1 ELISA Kits from leading suppliers on Biocompare. View specifications, ... ADP ribosylation factor interacting protein 1 ELISA Kits. The ELISA (enzyme-linked immunosorbent assay) is a widely used ... Bovine ADP-ribosylation factor-like protein 6-interacting protein 1 (ARL6IP1) ELISA Kit ... Canine ADP-ribosylation factor-like protein 6-interacting protein 1 (ARL6IP1) ELISA Kit ...
anti-ADP-Ribosylation Factor GTPase Activating Protein 2 Antibodies * anti-ADP-Ribosylation Factor GTPase Activating Protein 3 ... ADP-RIBOSYLATION FACTOR, ADP-ribosylation factor 1, ADP-RIBOSYLATION FACTOR 1A, arf1, arf2, arf3, ATARF, ATARF1, ATARFA1A, ... Vascular endothelial growth factor receptor-2 (show KDR Antibodies) activates ADP-ribosylation factor 1 to promote endothelial ... anti-ADP-Ribosylation Factor Guanine Nucleotide-Exchange Factor 1(brefeldin A-Inhibited) Antibodies ...
ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited)Imported. ,p>Information which has been ... tr,I3K9S1,I3K9S1_ORENI ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) OS=Oreochromis ... ADP-ribosylation factor guanine nuc.... ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) ... 1.10.1000.11, 1 hit. InterProi. View protein in InterPro. IPR016024 ARM-type_fold. IPR032629 DCB_dom. IPR015403 Sec7_C. ...
GDP/GTP exchange modulates the interaction of the small G-protein ADP-ribosylation factor-1 with membrane lipids: if ARF(GDP) ... N-terminal hydrophobic residues of the G-protein ADP-ribosylation factor-1 insert into membrane phospholipids upon GDP to GTP ... from vesicles by factors of 7 and 100, respectively. This strongly suggests that, upon GDP/GTP exchange, the N-terminal helix ... Antonny B1, Beraud-Dufour S, Chardin P, Chabre M.. Author information. 1. CNRS, Institut de Pharmacologie Moleculaire et ...
ADP-ribosylation factor GTPase-activating protein 2 isoform 1Imported. ,p>Information which has been imported from another ... tr,F7A1C9,F7A1C9_CALJA ADP-ribosylation factor GTPase-activating protein 2 isoform 1 OS=Callithrix jacchus OX=9483 GN=ARFGAP2 ... ADP-ribosylation factor GTPase-activating protein 3. ). HUMAN. 516. UniRef50_Q9NP61. ADP-ribosylation factor GTPase-activating ... 3.30.40.160, 1 hit. InterProi. View protein in InterPro. IPR037278 ARFGAP/RecO. IPR001164 ArfGAP_dom. IPR038508 ArfGAP_dom_sf ...
OMIM: ADP-RIBOSYLATION FACTOR 1; ARF1*Gene Ontology: Arf1 *Mouse Phenome DB: Arf1 *UCSC: Chr.11:59,211,412-59,228,267(-)*IMPC: ... ADP-ribosylation factor 1. Gene nomenclature, locus information, and GO, OMIM, and PMID associations are updated daily from MGI ... Vega: OTTMUSG6340 (Arf1, ADP-ribosylation factor 1)*CCDS: 24765, 24765.1* ... Question? Comments? For Mice, Cells, and germplasm please contact us at [email protected], US 1-888-KOMP-MICE or International + ...
What is ADP-ribosylation factor-like 2-like 1? Meaning of ADP-ribosylation factor-like 2-like 1 medical term. What does ADP- ... Looking for online definition of ADP-ribosylation factor-like 2-like 1 in the Medical Dictionary? ADP-ribosylation factor-like ... redirected from ADP-ribosylation factor-like 2-like 1) ARL13B. A gene on chromosome 3q11.1 that encodes a GTP-binding protein ... ADP-Ribosylation Factor 4-Like. *ADP-Ribosylation Factor 5. *ADP-ribosylation factor 5, OTTHUMP00000024864, OTTHUMP00000196795 ...
Top performende anti-Maus ADP-Ribosylation Factor 1 Antikörper für Immunofluorescence (Paraffin-embedded Sections) (IF (p)) ... Recommended ADP-Ribosylation Factor 1 Antibody (geliefert von: Anmelden zum Anzeigen ) Antigen ADP-Ribosylation Factor 1 (ARF1 ... anti-ADP-Ribosylation Factor 1 Antikörper * anti-Maus ADP-Ribosylation Factor 1 Antikörper für Immunofluorescence (Paraffin- ... anti-Maus ADP-Ribosylation Factor 1 Antikörper für Immunofluorescence (Paraffin-embedded Sections). ...
ADP-Ribosylation Factor 1 Antikörper für Immunofluorescence (Paraffin-embedded Sections) (IF (p)) vergleichen & kaufen. ... Recommended ADP-Ribosylation Factor 1 Antibody (geliefert von: Anmelden zum Anzeigen ) Antigen ADP-Ribosylation Factor 1 (ARF1 ... anti-ADP-Ribosylation Factor 1 Antikörper * anti-Ratte (Rattus) ADP-Ribosylation Factor 1 Antikörper für Immunofluorescence ( ... anti-Ratte (Rattus) ADP-Ribosylation Factor 1 Antikörper für Immunofluorescence (Paraffin-embedded Sections). ...
Recombinant Human ADP-ribosylation Factor 1, His-tagged. Download Datasheet See All ARF1 Products. Bring this labeled protein ... ARF1, also known as ADP-ribosylation factor 1, is a small guanine nucleotide-binding protein that enhances the enzymatic ... Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated ...
Mouse ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited) (Arfgef1), (10ug), 10 µg. ... Home » cDNA » Mouse cDNA » Arfgef1 (untagged) - Mouse ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A- ... Properties for Arfgef1 (untagged) - Mouse ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited) ( ... MC224976 Arfgef1 (untagged) - Mouse ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited) ( ...
With BFA, the association rate of the abortive complex is drastically reduced by a factor of 42, and by contrast, the 15-fold ... potentiates by a factor of 2 the allosteric effect of GDP. Then we explored the uncompetitive inhibitory mechanism of brefeldin ... The GDP/GTP nucleotide exchange of Arf1 is catalyzed by nucleotide exchange factors (GEF), such as Arno, which act through ... Kinetics of Interaction between ADP-ribosylation Factor-1 (Arf1) and the Sec7 Domain of Arno Guanine Nucleotide Exchange Factor ...
ADP-ribosylation factor GTPase-activating protein 1 (ARFGAP1) Recombinant Protein-NP_001268411.1 (MBS1301288) product datasheet ... ADP-ribosylation factor GTPase-activating protein 1 isoform c NCBI Official Synonym Full Names ADP-ribosylation factor GTPase ... ADP-ribosylation factor GTPase-activating protein 1 (ARFGAP1), Recombinant Protein. Also Known As Recombinant Human ADP- ... ADP-ribosylation factor GTPase-activating protein 1 UniProt Synonym Protein Names ADP-ribosylation factor 1 GTPase-activating ...
"ADP-Ribosylation Factor 1" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical ... ADP-RIBOSYLATION FACTOR 1 is involved in regulating intracellular transport by modulating the interaction of coat proteins with ... This graph shows the total number of publications written about "ADP-Ribosylation Factor 1" by people in this website by year, ... Below are the most recent publications written about "ADP-Ribosylation Factor 1" by people in Profiles. ...
Ver más] The small GTP-binding protein ADP-ribosylation factor 1 (ARF1) is an essential component of the molecular machinery ... The small GTP-binding protein ADP-ribosylation factor 1 (ARF1) is an essential component of the molecular machinery that ... Effect of protein kinase a activity on the association of ADP-ribosylation factor 1 to Golgi membranes. ...
HCA RNA Cell Line for ADP-ribosylation factor-like protein 6-interacting protein 1. ... Compartment GO Terms for ADP-ribosylation factor-like protein 6-interacting protein 1. ...
Recombinant Human ADP-ribosylation factor-like protein 2 Protein. Synthesized in e. coli. Protein Tag: GST. Purity: Greater ... Recombinant Human ADP-ribosylation factor-like protein 1. $ 398.00 - $ 1,728.00. Please Select Product Options Below To View ... Be the first to review "Recombinant Human ADP-ribosylation factor-like protein 1" Cancel reply. Your email address will not be ... Human ADP-ribosylation factor-like protein 2 Recombinant Protein Product Attributes. Product Type: Recombinant Protein ...
Hara Y, Fukaya M, Hayashi K, Kawauchi T, Nakajima K, Sakagami H. ADP ribosylation factor 6 regulates neuronal migration in the ... Hara, Y., Fukaya, M., Hayashi, K., Kawauchi, T., Nakajima, K., & Sakagami, H. (2016). ADP ribosylation factor 6 regulates ... ADP ribosylation factor 6 (Arf6) is a critical small GTPase that regulates membrane trafficking between the plasma membrane and ... Hara, Y, Fukaya, M, Hayashi, K, Kawauchi, T, Nakajima, K & Sakagami, H 2016, ADP ribosylation factor 6 regulates neuronal ...
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
ADP ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily. ARF family ... The small ADP ribosylation factor (Arf) GTP-binding proteins are major regulators of vesicle biogenesis in intracellular ... Kahn RA, Kern FG, Clark J, Gelmann EP, Rulka C (1991). "Human ADP-ribosylation factors. A functionally conserved family of GTP- ... Amor JC, Harrison DH, Kahn RA, Ringe D (1994). "Structure of the human ADP-ribosylation factor 1 complexed with GDP". Nature. ...
ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide- ... ADP ribosylation factor 1. Enable Javascript to view the expand/collapse boxes.. Open All Close All ... Microbial infection) Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. ... binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking ...
ADP-Ribosylation Factor 1 Pseudogene, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards ... LOC101060021 (ADP-Ribosylation Factor 1 Pseudogene) is a Pseudogene. Additional gene information for LOC101060021 Gene ... Transcription Factor. Binding Sites. Gene Targets. GH12J058396. Enhancer. 0.3. Ensembl 600.7. +1.2. 1186. 0.4. CEBPB JUND ... 1 5 6 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z ... ADP-Ribosylation Factor 1 Pseudogene. GCID:. GC12P058364 GIFtS: ...
ADP-Ribosylation Factor 6 Expression and Activation Are Reduced in Myometrium in Complicated Pregnancies by: Venkat, ... ADP-ribosylation factor 6 regulates endothelin-1-induced lipolysis in adipocytes by: Venkat, Kanamarlapudi Published: (2014) ... an ADP-Ribosylation Factor 6 GTPase Activating Protein, Inhibits 2-Adrenoceptor Internalization / J. Lawrence; S. J. Mundell; H ... an ADP-Ribosylation factor 6 GTPase activating protein, inhibits beta(2)-Adrenoceptor internalization,journal = Molecular ...
ADP Ribosylation Factor 1 Pseudogene 2, including: function, proteins, disorders, pathways, orthologs, and expression. ... ARF1P2 (ADP Ribosylation Factor 1 Pseudogene 2) is a Pseudogene. Additional gene information for ARF1P2 Gene ... Transcription Factor. Binding Sites. Gene Targets. GH12J120840. Enhancer. 0.7. dbSUPER 600.7. +1.7. 1743. 6.6. PRDM1 ZNF24 ... 1 5 6 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z ... Transcription Factor Targets and HOMER Transcription for ARF1P2 ...
The GGA (Golgi-localized, -ear containing, ADP-ribosylation factor binding) family of adaptor proteins serve a similar role. ... A cargo/PACS-1/AP-1A complex is necessary to drive the appropriate transport of several cargo proteins within the regulated ... We review the functions of AP-1A, PACS-1 and GGAs in facilitating the retrieval of proteins from immature SGs and review ... AP-1A recruitment to membranes can be modulated by PACS-1 (Phosphofurin Acidic Cluster Sorting protein 1), a cytosolic protein ...
Frank S, Upender S, Hansen SH, Casanova JE (1998). "ARNO is a guanine nucleotide exchange factor for ADP-ribosylation factor 6 ... ADP-ribosylation factor 1 is a protein that in humans is encoded by the ARF1 gene. ADP-ribosylation factor 1 (ARF1) is a member ... 1991). "ADP-ribosylation factor is a subunit of the coat of Golgi-derived COP-coated vesicles: a novel role for a GTP-binding ... "Entrez Gene: ARF1 ADP-ribosylation factor 1". Mitchell R, Robertson DN, Holland PJ, Collins D, Lutz EM, Johnson MS (September ...
anti-A2MP1 (ADP-ribosylation factor 1) antibody *. Applications: WB, ELISA. *. Reactivity: Human (Hu) ... Ly-6G/Ly-6C (Gr-1) Antibody for Flow Cytometry Using this antibody you can stain for granulocytes and macrophages and analyse ... A2MP1 encodes the human gene, Alpha-2-macroglobulin pseudogene 1. It may also be known as: alpha-2-macroglobulin pseudogene 1; ...
  • The protein encoded by this gene belongs to the ARL (ADP-ribosylation factor-like) family of proteins, which are structurally related to ADP-ribosylation factors (ARFs). (nih.gov)
  • GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. (rcsb.org)
  • Your search returned 25 ADP ribosylation factor interacting protein 1 ELISA ELISA Kit across 2 suppliers. (biocompare.com)
  • Additionally we are shipping ADP-Ribosylation Factor 1 Proteins (28) and many more products for this protein. (antibodies-online.com)
  • Lu, Horstmann, Ng, Hong: Regulation of Golgi structure and function by ARF-like protein 1 (Arl1). (antibodies-online.com)
  • N-terminal hydrophobic residues of the G-protein ADP-ribosylation factor-1 insert into membrane phospholipids upon GDP to GTP exchange. (nih.gov)
  • GDP/GTP exchange modulates the interaction of the small G-protein ADP-ribosylation factor-1 with membrane lipids: if ARF(GDP) is mostly soluble, ARF(GTP) binds tightly to lipid vesicles. (nih.gov)
  • A gene on chromosome 3q11.1 that encodes a GTP-binding protein of the ADP-ribosylation factor-like family, which participates in small GTPase-mediated signal transduction. (thefreedictionary.com)
  • ARF1, also known as ADP-ribosylation factor 1, is a small guanine nucleotide-binding protein that enhances the enzymatic activities of cholera toxin. (creativebiomart.net)
  • This protein is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). (mybiosource.com)
  • The small GTP-binding protein ADP-ribosylation factor 1 (ARF1) is an essential component of the molecular machinery that catalyzes the formation of membranebound transport intermediates. (us.es)
  • Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). (enquirebio.com)
  • A DNA sequence encoding the Homo sapiens (Human) ADP-ribosylation factor-like protein 2, was expressed in the hosts and tags indicated. (enquirebio.com)
  • Rescue experiments with separation-of-function Arf6 mutants identified Rab11 familyinteracting protein 3 (FIP3)/Arfophilin-1, a dual effector for Arf6 and Rab11, as a downstream effector of Arf6 in migrating neurons. (elsevier.com)
  • AP-1A recruitment to membranes can be modulated by Phosphofurin Acidic Cluster Sorting protein 1 (PACS-1), a cytosolic protein which interacts with both AP-1A and cargo that has been phosphorylated by casein kinase II. (frontiersin.org)
  • Structural studies of Arf1 and Arf6 have revealed that although these proteins feature the switch 1 and 2 conformational changes, they depart from other small GTP-binding proteins in that they use an additional, unique switch to propagate structural information from one side of the protein to the other. (wikipedia.org)
  • ADP-ribosylation factor 1 is a protein that in humans is encoded by the ARF1 gene. (wikipedia.org)
  • Putative target protein of ADP-ribosylation factor. (hoelzel-biotech.com)
  • MS/MS spectrum of the identified peptide (FLQGIQLNTILPDAR) corresponding to the protein LAMP-1 from ovarian OVCAR3 cells treated with anti- or pro-growth stimuli for 48 h. (mcponline.org)
  • Regulation of growth factor receptor degradation by ADP-ribosylation factor domain protein (ARD) 1. (nih.gov)
  • Below are the list of possible ADP-ribosylation factor-like protein 6-interacting protein products. (mybiosource.com)
  • Also known as ADP-ribosylation factor-like protein 6-interacting protein 1 (ARL-6-interacting protein 1) (Aip-1) (Apoptotic regulator in the membrane of the endoplasmic reticulum). (mybiosource.com)
  • Also known as ADP-ribosylation factor-like protein 6-interacting protein 4 (ARL-6-interacting protein 4) (Aip-4). (mybiosource.com)
  • ARL-6-interacting protein 1. (or.jp)
  • The protein encoded by this gene belongs to the ARF-like (ADP ribosylation factor-like) sub-family of the ARF family of GTP-binding proteins which are involved in regulation of intracellular traffic. (genscript.com)
  • ADP-Ribosylation Factor Interacting Protein 1 (ARFIP1) contains 1 AH domain and is a putative target protein of ADP-ribosylation factor. (prospecbio.com)
  • ARFIP1 (NP_001020766.1, 1 a.a. ~ 373 a.a) full-length human protein. (abnova.com)
  • Because hypertriglyceridemia is an independent risk factor for atherosclerosis, the observation that RORα regulates apoA-V 5 may indicate that this protein must also be taken into account when explaining the antiatherogenic effect of RORα. (ahajournals.org)
  • Several proteins have been identified on these droplets, the most well known being the PAT domain proteins, 1-3 including the perilipins, adipocyte differentiation-related protein (ADRP or adipophilin) and Tip 47. (ahajournals.org)
  • 7. The expression vector according to claim 5, wherein the recombinant nucleic acid according to claim 1 further comprises a protein coding sequence that encodes a reporter protein or a therapeutic protein. (freepatentsonline.com)
  • 100 µg, ABP-PAB-11315]] The guanine nucleotide binding protein (G protein) alpha inhibiting activity polypeptide 1 (GNAI1) is acomponent of the pertussis toxin-sensitive heterotrimeric. (allelebiotech.com)
  • 100 μg, ABP-PAB-01107]] (100 μg) The ADP-ribosylation factor related protein 1 (ARFRP1) is a membrane-associated GTP-ase and localizes to the plasma membrane. (allelebiotech.com)
  • ARFRP1 is a 25-kDa protein which is related to the ADP-ribosylation factor (ARF) and. (allelebiotech.com)
  • To identify which segment of CgA (chromogranin A) is important to induce the formation of such granule-like structures, a series of deletion constructs fused to either GFP (green fluorescent protein) or a short epitope tag was expressed in COS-1 fibroblast cells and analysed by fluorescence and electron microscopy and pulse-chase labelling. (portlandpress.com)
  • Their activity is controlled by specific guanine exchange factors (ARF-GEFs) that activate ARFs by catalyzing a GDP-to -GTP exchange that ultimately recruits vesicle coat proteins, and by ARF- GTPase ACTIVATING PROTEINs that hydrolyze GTP back to GDP for protein inactivation ( Singh and Jürgens, 2018 ). (plantcell.org)
  • A target of phosphatidylinositol 3,4,5-trisphosphate with a zinc finger motif similar to that of the ADP-ribosylation-factor GTPase-activating protein and two pleckstrin homology domains. (embl-heidelberg.de)
  • The activation of the human polymorphonuclear neutrophils by the vast majority of the agents inducing its directed migration to sites of injury or infection (chemotactic factors and chemokines) is mediated by their interaction with G protein-coupled receptors (GPCRs) 3 ( 1 ). (jimmunol.org)
  • Ubiquitous GTP-binding protein, approximately 20 kD, N-myristoylated, stimulates cholera toxin ADP-ribosylation. (academic.ru)
  • Mediates binding of non-clathrin coated vesicles and AP1 (adaptor-protein 1) of clathrin-coated vesicles to Golgi membranes. (academic.ru)
  • ADP-RIBOSYLATION FACTOR 1 is involved in regulating intracellular transport by modulating the interaction of coat proteins with organelle membranes in the early secretory pathway. (sickkids.ca)
  • The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. (nih.gov)
  • The trafficking of granule membrane proteins can be controlled by both luminal and cytosolic factors. (frontiersin.org)
  • A cargo/PACS-1/AP-1A complex is necessary to drive the appropriate transport of several cargo proteins within the regulated secretory pathway. (frontiersin.org)
  • We review the functions of AP-1A, PACS-1, and GGAs in facilitating the retrieval of proteins from immature SGs and review examples of cargo proteins whose trafficking within the regulated secretory pathway is governed by APs. (frontiersin.org)
  • ADP ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily. (wikipedia.org)
  • The small ADP ribosylation factor (Arf) GTP-binding proteins are major regulators of vesicle biogenesis in intracellular traffic. (wikipedia.org)
  • In the GTP-bound form of these proteins, the interswitch undergoes a two-residue register shift that pulls switch 1 and switch 2 up, restoring an active conformation that can bind GTP. (wikipedia.org)
  • GTPase activating proteins (GAPs) force ARF to hydrolyze bound GTP to GDP, and Guanine nucleotide exchange factors force ARF to adopt a new GTP molecule in place of a bound GDP. (wikipedia.org)
  • There are currently 6 known mammalian ARF proteins, which are divided into three classes of ARFs: class 1: ARF1, ARF2, ARF3 class 2: ARF4, ARF5 class 3: ARF6. (wikipedia.org)
  • Among the small GTP-binding proteins, ARF1 (ADP-ribosylation factor 1) and SAR1 (Secretion-Associated RAS super family 1) are commonly conserved among all eukaryotes with respect to both their functional and sequential characteristics. (mdpi.com)
  • Guanine-nucleotide exchange factors (GEFs), GDP dissociation inhibitors (GDIs), and GTPase-activating proteins are regulators of small GTP-binding proteins ( Figure 1 ). (mdpi.com)
  • The ADP-ribosylation factor (Arf) family comprises a group of structurally and functionally conserved 21 kDa proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. (novusbio.com)
  • Arf has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D. The Arf family is divided functionally into the Arf and the Arf-like (Arl) proteins. (novusbio.com)
  • ARL1 is part of the ARL (ADP-ribosylation factor-like) family of proteins, which are structurally associated to ADP-ribosylation factors (ARFs). (prospecbio.com)
  • Upon DNA break induction by irradiation or alkylating agents, PARP-1 binds to DNA break sites and, by using NAD + , catalyzes poly(ADP-ribose) formation onto nuclear acceptor proteins [ 1 , 2 ]. (hindawi.com)
  • We reported previously that RalA interacts with PLD activating ADP ribosylation factor (ARF) proteins. (asm.org)
  • Functional interaction of the active zone proteins Munc 13-1 and RIM 1 in synaptic vesicle priming. (mpg.de)
  • ADP-RIBOSYLATION FACTOR (ARF) proteins play essential roles in vesicle trafficking by regulating the formation of membrane vesicles that move cargo throughout the cell. (plantcell.org)
  • ADP-ribosylation factors (ARFs) are approximately 20-kDa guanine nucleotide-binding proteins recognized as critical components in intracellular vesicular transport and phospholipase D activation. (embl-heidelberg.de)
  • Arf6 - ADP ribosylation factor 6 (ARF6) is a member of the ADP ribosylation factor family of GTP binding proteins. (academic.ru)
  • ADP ribosylation factor 6 (Arf6) is a critical small GTPase that regulates membrane trafficking between the plasma membrane and endosomes. (elsevier.com)
  • The small GTPase ADP-Ribosylation Factor 1 mediates the sensitivity of triple negative breast cancer cells to EGFR tyrosine kinase inhibitors. (ptglab.com)
  • Title: ARFRP1 functions upstream of ARL1 and ARL5 to coordinate recruitment of distinct tethering factors to the trans-Golgi network. (nih.gov)
  • We report here that 2-methylcoprophilinamide (M- COPA (show COPA Antibodies )), a compound that induces disassembly of the Golgi apparatus by inactivating ADP-ribosylation factor 1 (Arf1 ), suppresses Stx (show ST8SIA2 Antibodies )-induced apoptosis. (antibodies-online.com)
  • The Golgi-localized, γ-ear containing, ADP-ribosylation factor binding (GGA) family of APs serve a similar role. (frontiersin.org)
  • In 84.6 ± 3.6% (mean ± SEM) of cells expressing YFP-PH, HA did not reach the apical membrane ( Fig. 1 , outlined cells and cells with asterisks) but colocalized with YFP-PH in the Golgi. (rupress.org)
  • Moreover, Exo1 induces the rapid release of ADP-ribosylation factor (ARF) 1 from Golgi membranes but has less effect on the organization of the trans-Golgi network. (axonmedchem.com)
  • ARFs, described as activators of cholera toxin (CT) ADP-ribosyltransferase activity, regulate intracellular vesicular membrane trafficking, and stimulate a phospholipase D (PLD) isoform. (nih.gov)
  • This antibody will label ARF 1 isoform 1, 2, 3 and 4. (abcam.com)
  • Immunocytochemistry/ Immunofluorescence: ARF1 Antibody (1D9) [NB300-505] - Analysis of ADP-Ribosylation Factor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. (novusbio.com)
  • ADP-ribosylation factor 3 antibody. (acris-antibodies.com)
  • ARF3 antibody (TA308849) dilution: 1:500. (acris-antibodies.com)
  • Immunofluorescence analysis of methanol-fixed HeLa, using ARF3(TA308849) antibody at 1:500 dilution. (acris-antibodies.com)
  • HeLa cells were subjected to SDS PAGE followed by western blot with 10790-1-AP (ARF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. (ptglab.com)
  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10790-1-AP (ARF1 antibody) at dilution of 1:200 (under 10x lens. (ptglab.com)
  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10790-1-AP (ARF1 antibody) at dilution of 1:200 (under 40x lens. (ptglab.com)
  • Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 10790-1-AP (ARF1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). (ptglab.com)
  • Bos taurus ADP-ribosylation factor-like 1 (ARL1), mRNA. (bio-rad.com)
  • Lu & Werner in "The complete cDNA sequence of mouse elongation factor 1 alpha (EF 1 alpha) mRNA" (NAR, 1989, vol. 17, p. 442). (freepatentsonline.com)
  • PIP2 may be hydrolyzed by phospholipase C (PLC) to generate inositol-1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) second messengers or phosphorylated in the D3 position by class 1 phosphatidylinositol 3-kinase (PI3K) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). (frontiersin.org)
  • LOC101060021 (ADP-Ribosylation Factor 1 Pseudogene) is a Pseudogene. (genecards.org)
  • A2MP1 encodes the human gene, Alpha-2-macroglobulin pseudogene 1. (biocompare.com)
  • ARFIP1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 364 amino acids (1-341 a.a) and having a molecular mass of 41.0kDa. (hoelzel-biotech.com)
  • 2. The recombinant nucleic acid of claim 1, wherein said first region and said second region are in proximity to each other or are adjacent to each other or overlap with each other or one encompasses the other. (freepatentsonline.com)
  • 4. The recombinant nucleic acid of claim 1, wherein the nucleic acid is DNA, RNA, or PNA. (freepatentsonline.com)
  • 5. An expression vector comprising the recombinant nucleic acid according to claim 1. (freepatentsonline.com)
  • The µ1 subunit of AP-1 in Arabidopsis ( Arabidopsis thaliana ) was recently identified at the TGN / EE and shown to be essential for cytokinesis. (plantphysiol.org)
  • Glia maturation factor-γ mediates neutrophil chemotaxis. (nih.gov)
  • Translational efficiency is regulated by the length of the 3′ untranslated region," Molecular and Cellular Biology, 1996, vol. 16, No. 1, pp. 146-156. (freepatentsonline.com)
  • Dissection of Arabidopsis ADP-RIBOSYLATION FACTOR 1 function in epidermal cell polarity. (semanticscholar.org)
  • Additionally, RalA, PLD1, or PLD2 could cooperate with either c-Src or the epidermal growth factor (EGF) receptor (EGFR) to transform rat fibroblasts ( 38 , 44 ). (asm.org)
  • Epidermal growth factor receptor - This article is about a cell surface receptor. (academic.ru)
  • 100 µg, ABP-PAB-11610]] (100 µg) PDPK1 is a mediator of several signaling pathways coupled to growth factor receptor activation in human cancers. (allelebiotech.com)
  • RalA has also been implicated in the activation of PLD by phorbol esters ( 60 ) and by growth factor receptor tyrosine kinases ( 30 , 73 ). (asm.org)
  • Expression of apoA-V is increased by interaction of the heterodimer PPARα (peroxisome proliferator-activator receptor)/RXR (retinoid X receptor) with a "Direct Repeat"1 (DR1) sequence in the apoA-V promoter. (ahajournals.org)
  • Prieurianin/endosidin 1 is an actin-stabilizing small molecule identified from a chemical genetic screen for circadian clock effectors in Arabidopsis thaliana. (semanticscholar.org)
  • The 3′-end of the human β-actin gene enhances activity of the β-actin expression vector system: construction of improved vectors," Journal of Biochemical and Biophysical Methods, 1997, vol. 36, No. 1, pp. 63-72. (freepatentsonline.com)
  • ADP-Ribosylation Factor 1" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (sickkids.ca)
  • This page contains a compilation of Rel/NF-kappaB target genes that is derived from the survey paper Activators and target genes of Rel/NF-kappaB transcription factors (Pahl, Oncogene 1999), the Rel/NF-kappaB transcription factors website of TD Gilmore, and additional search with PubMed. (lifl.fr)
  • There are seventeen genes encoding PARP enzymes in mammalians, including PARP-1, PARP-2, PARP-3, and PARP-4/vPARP: PARP-5/Tankyrases-1 and PARP-6/Tankyrases-2 [ 7 ]. (hindawi.com)
  • Active RalA-PLD complexes also contain ADP ribosylation factor (ARF) GTPases ( 47 ), which activate PLD1 ( 7 , 15 , 27 ). (asm.org)
  • ARF1 - ADP ribosylation factor 1, also known as ARF1, is a human gene. (academic.ru)
  • 1 Because hypertriglyceridemia is an independent risk factor for the development of atherosclerosis and cardiovascular diseases, elucidation of the mechanism behind the regulation of apoA-V is of great importance. (ahajournals.org)
  • 3,4 In this issue of Arteriosclerosis, Thrombosis, and Vascular Biology , Genoux et al 5 show that the retinoid-related orphan receptors alpha (RORα) 1 and 4 are also involved in the regulation of the apoA-V gene by interaction with the DR1 binding site. (ahajournals.org)
  • Moreover, it has been shown to inhibit inflammatory responses in smooth muscle cells by transcriptional regulation of the I-κBα gene, which codes for the inhibitor of NF-κB transcription factor activity. (ahajournals.org)
  • Tyrosine phosphorylation events play major roles in the initiation and regulation of several functional responses of human neutrophils stimulated by chemotactic factors such as the bacterially derived tripeptide formylmethionyl-leucyl-phenylalanine (fMet-Leu-Phe). (jimmunol.org)
  • LAMP-1 is not stained in normal ovarian tissue sections ( B-E ). Sections stained with mouse IgG as a negative control. (mcponline.org)
  • LAMP-1 is expressed in serous epithelial ovarian adenocarcinoma tissue sections ( G-J ). LAMP-1 expression is specifically observed in the cytoplasm of epithelial cells. (mcponline.org)
  • LAMP-1 expression in ovarian carcinoma tissue cores. (mcponline.org)
  • ADP-Ribosylation Factor 6 Expression and Activation Are Reduced in Myometrium in Complicated Pregnancies by: Venkat, Kanamarlapudi, et al. (swan.ac.uk)
  • PIP2 also serves as a precursor for second messengers inositol triphosphate (IP3), diacylglycerol (DAG), and phosphatidylinositol 3,4,5-triphosphate (PIP3) (Figure 1 B), which are essential for the activation of the signaling pathways regulating efficient cytokine production, cell cycle progression, survival, and T cell metabolism ( 5 ). (frontiersin.org)
  • ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. (nih.gov)
  • Phosphatidylinositol 4,5-biphosphate kinases (PIP5Ks) are a family of isoenzymes that mediate the phosphorylation of phosphatidylinositol 4-phosphate on the D5 position of the inositol ring, thus inducing the production of phosphatidylinositol 4,5-biphosphate (PIP2) ( 1 ) (Figure 1 ). (frontiersin.org)
  • Chemotactic Factor-Induced Recruitment and Activation of Tec Family Kinases in Human Neutrophils. (jimmunol.org)
  • Of particular relevance to the subject of the present investigation, stimulation of human neutrophils by chemotactic factors has very recently been observed to be associated with the translocation and activation of the PH domain-containing tyrosine kinases of the Tec family termed Tec, Btk and Bmx ( 16 ). (jimmunol.org)
  • PBB Summary section title = summary text = ADP ribosylation factor 1 (ARF1) is a member of the human ARF gene family. (academic.ru)
  • Munc18-1 promotes larger dense-core vesicle docking. (mpg.de)
  • Grube and Bürkle [ 14 ] first described a positive correlation between the poly(ADP-ribosyl)ation capacity of mononuclear blood cells with longevity of mammalian species. (hindawi.com)