A membrane-bound or cytosolic enzyme that catalyzes the synthesis of CYCLIC ADP-RIBOSE (cADPR) from nicotinamide adenine dinucleotide (NAD). This enzyme generally catalyzes the hydrolysis of cADPR to ADP-RIBOSE, as well, and sometimes the synthesis of cyclic ADP-ribose 2' phosphate (2'-P-cADPR) from NADP.
A bifunctional enzyme that catalyzes the synthesis and HYDROLYSIS of CYCLIC ADP-RIBOSE (cADPR) from NAD+ to ADP-RIBOSE. It is a cell surface molecule which is predominantly expressed on LYMPHOID CELLS and MYELOID CELLS.
An enzyme of the lyase class that catalyzes the formation of CYCLIC AMP and pyrophosphate from ATP. EC 4.6.1.1.
A pentose active in biological systems usually in its D-form.
An enzyme that catalyzes the conversion of GTP to 3',5'-cyclic GMP and pyrophosphate. It also acts on ITP and dGTP. (From Enzyme Nomenclature, 1992) EC 4.6.1.2.
A coenzyme composed of ribosylnicotinamide 5'-diphosphate coupled to adenosine 5'-phosphate by pyrophosphate linkage. It is found widely in nature and is involved in numerous enzymatic reactions in which it serves as an electron carrier by being alternately oxidized (NAD+) and reduced (NADH). (Dorland, 27th ed)
One of the virulence factors produced by virulent BORDETELLA organisms. It is a bifunctional protein with both ADENYLYL CYCLASES and hemolysin components.
The rate dynamics in chemical or physical systems.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
Adenosine 5'-(trihydrogen diphosphate). An adenine nucleotide containing two phosphate groups esterified to the sugar moiety at the 5'-position.
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.

Purification and characterization of ADP-ribosyl cyclase from Euglena gracilis. (1/575)

ADP-ribosyl cyclase, which catalyzes the conversion from NAD+ to cyclic adenosine diphosphoribose (cADPR), is proposed to participate in cell cycle regulation in Euglena gracilis. This enzyme, which was found as a membrane-bound protein, was purified almost the homogeneity after solubilization with deoxycholate, and found to be a monomeric protein with a molecular mass of 40 kDa. Its Km value for NAD+ was estimated to be 0.4 mM, and cADPR, a product of the enzyme, inhibited the enzyme competitively with respect to NAD+ whereas another product, nicotinamide, showed noncompetitive (mixed-type) inhibition. In contrast to mammalian CD38 and BST-1, Euglena ADP-ribosyl cyclase lacked cADPR hydrolase activity.  (+info)

Characterization of viral dynamics in human immunodeficiency virus type 1-infected patients treated with combination antiretroviral therapy: relationships to host factors, cellular restoration, and virologic end points. (2/575)

Biphasic plasma viral decays were modeled in 48 patients treated with ritonavir, zidovudine, and lamivudine. Estimated first- and second-phase decay rates were d1 as 0.47/day and d2 as 0.04/day. Interpatient differences in both decay rates were significant. The d1 was directly correlated with baseline CD4+, CD4+CD28+, and CD8+CD28+ T lymphocyte counts (P<.05) and inversely correlated with baseline virus load (P=.044) and the magnitude of CD4+ and CD8+ T lymphocyte recovery (P<.01). The d2 was directly correlated with baseline percentage of CD8+ T lymphocytes (P=.023), the CD8+CD38+ cell number (P=.024), and the level of IgG that binds to human immunodeficiency virus (HIV) type 1 gp120 (P=.02). Viral decay rates were not predictive of treatment failure or durability of viral suppression. These exploratory findings are consistent with a model in which immunologic factors contribute to elimination of HIV-infected cells and suggest a dynamic interplay between regulation of HIV expression and lymphocyte activation and recovery.  (+info)

Shorter survival in advanced human immunodeficiency virus type 1 infection is more closely associated with T lymphocyte activation than with plasma virus burden or virus chemokine coreceptor usage. (3/575)

To define predictors of survival time in late human immunodeficiency virus type 1 (HIV-1) disease, long- and short-duration survivors were studied after their CD4+ T cells fell to +info)

IL-5 induces IgG1 isotype switch recombination in mouse CD38-activated sIgD-positive B lymphocytes. (4/575)

Mouse B cells express CD38, whose ligation by anti-CD38 Ab induces their proliferation and protection from apoptosis. We previously showed that stimulation of mouse splenic B cells with IL-5 together with CS/2, an anti-mouse CD38 mAb, induces production of IgG1 and IgM. Here we examined the role of IL-5 and CS/2 in the expression of germline gamma1 transcripts and the generation of reciprocal products forming DNA circles as byproducts of mu-gamma1 switch recombination. By itself, CS/2 induced significant expression of germline gamma1 transcripts in splenic naive B cells, whereas IL-5 neither induced nor enhanced germline gamma1 expression. Increased cellular content of reciprocal product, which is characteristic of mu-gamma1 recombination, was not observed after culturing B cells with CS/2, but increased reciprocal product, along with high levels of lgG1 secretion, was found when B cells were cultured with CS/2 plus IL-5. Although IL-4 did not, by itself, induce mu-gamma1 recombination in B cells stimulated with CS/2, in conjunction with CS/2 plus IL-5, IL-4 dramatically enhanced sterile gamma1 transcription and IgG1 production. These results demonstrate that CD38 ligation induces only germline gamma1 transcription and that IL-5 promotes both mu-gamma1 switch recombination and lgG1 secretion in an IL-4-independent manner.  (+info)

Stable transduction of quiescent CD34(+)CD38(-) human hematopoietic cells by HIV-1-based lentiviral vectors. (5/575)

We compared the efficiency of transduction by an HIV-1-based lentiviral vector to that by a Moloney murine leukemia virus (MLV) retroviral vector, using stringent in vitro assays of primitive, quiescent human hematopoietic progenitor cells. Each construct contained the enhanced green fluorescent protein (GFP) as a reporter gene. The lentiviral vector, but not the MLV vector, expressed GFP in nondivided CD34(+) cells (45.5% GFP+) and in CD34(+)CD38(-) cells in G0 (12.4% GFP+), 48 hr after transduction. However, GFP could also be detected short-term in CD34(+) cells transduced with a lentiviral vector that contained a mutated integrase gene. The level of stable transduction from integrated vector was determined after extended long-term bone marrow culture. Both MLV vectors and lentiviral vectors efficiently transduced cytokine-stimulated CD34(+) cells. The MLV vector did not transduce more primitive, quiescent CD34(+)CD38(-) cells (n = 8). In contrast, stable transduction of CD34(+)CD38(-) cells by the lentiviral vector was seen for over 15 weeks of extended long-term culture (9.2 +/- 5.2%, n = 7). GFP expression in clones from single CD34(+)CD38(-) cells confirmed efficient, stable lentiviral transduction in 29% of early and late-proliferating cells. In the absence of growth factors during transduction, only the lentiviral vector was able to transduce CD34(+) and CD34(+)CD38(-) cells (13.5 +/- 2.5%, n = 11 and 12.2 +/- 9.7%, n = 4, respectively). The lentiviral vector is clearly superior to the MLV vector for transduction of quiescent, primitive human hematopoietic progenitor cells and may provide therapeutically useful levels of gene transfer into human hematopoietic stem cells.  (+info)

The metamorphosis of a molecule: from soluble enzyme to the leukocyte receptor CD38. (6/575)

Human CD38 is a 45-kDa type II membrane glycoprotein with an intricate pattern of expression in leukocytes, although evidence is accumulating of its quite widespread expression in cells of nonvascular origin. CD38 is a member of a nascent eukaryotic gene family encoding cytosolic and membrane-bound enzymes whose substrate is NAD, a coenzyme ubiquitously distributed in nature. Functionally, CD38 is an eclectic molecule with the ability not only to catalyze but also to signal, to mobilize calcium, and to adhere to itself, to hyaluronan, and to other ligands. Interaction with CD38 on various leukocyte subpopulations has profound though diverse consequences on their life-span, but these effects seem to be independent of the enzymatic activity of the molecule. CD38 challenges our expectations of a surface molecule and we must sift through its many guises to unmask its true nature.  (+info)

Evidence of a role for cyclic ADP-ribose in long-term synaptic depression in hippocampus. (7/575)

Ca2+ released from presynaptic and postsynaptic intracellular stores plays important roles in activity-dependent synaptic plasticity, including long-term depression (LTD) of synaptic strength. At Schaffer collateral-CA1 synapses in the hippocampus, presynaptic ryanodine receptor-gated stores appear to mobilize some of the Ca2+ necessary to induce LTD. Cyclic ADP-ribose (cADPR) has recently been proposed as an endogenous activator of ryanodine receptors in sea urchin eggs and several mammalian cell types. Here, we provide evidence that cADPR-mediated signaling pathways play a key role in inducing LTD. We show that biochemical production of cGMP increases cADPR concentration in hippocampal slices in vitro, and that blockade of cGMP-dependent protein kinase, cADPR receptors, or ryanodine-sensitive Ca2+ stores each prevent the induction of LTD at Schaffer collateral-CA1 synapses. A lack of effect of postsynaptic infusion of either cADPR antagonist indicates a probable presynaptic site of action.  (+info)

Expression of CD28 and CD38 by CD8+ T lymphocytes in HIV-1 infection correlates with markers of disease severity and changes towards normalization under treatment. The Swiss HIV Cohort Study. (8/575)

The relationship between blood CD8+ T lymphocyte subsets, as defined by CD28 and CD38 expression, and plasma viraemia and CD4+ T cells in HIV-1 infection was investigated. In a cross-sectional study of 46 patients with either no or stable anti-retroviral treatment, there was a strong negative correlation between the percentage of CD8+CD28- and the percentage of CD4+ T cells (r = -0.75, P < 0.0001), and a positive correlation between absolute numbers of CD8+CD28+ and CD4+ T cells (r = 0.56, P < 0.0001). In contrast, the expression of CD38 by CD8+ T lymphocytes correlated primarily with plasma viraemia (e.g. the percentage of CD38+ in CD8bright cells, r = 0.76, P < 0.0001). In the 6 months following triple therapy initiation in 32 subjects, there was a close correlation between changes (delta) in CD8+CD28+ or CD8+CD28- and in CD4+ T cells (e.g. delta % CD8+CD28+ versus delta % CD4+, r = 0.37, P = 0.0002; delta % CD8+CD28- versus delta % CD4+, r = -0.66, P < 0.0001). A marked decline of the number of CD8+ T cells expressing CD38 was also observed. These results suggest the existence of a T cell homeostasis mechanism operating in blood with CD4+ and CD8+CD28+ cells on the one hand, and with CD8+CD28- cells on the other. In addition, the percentage of CD38+ cells in CD8+ cells, generally considered an independent prognostic factor, could merely reflect plasma viral load.  (+info)

CD38 (ADP Ribosyl Cyclase I) Antibody - Without BSA and Azide, Mouse Monoclonal Antibody [Clone AT2 ] validated in IF, FC (AH12737-100), Abgent
Looking for online definition of ADP-ribosyl cyclase 2 in the Medical Dictionary? ADP-ribosyl cyclase 2 explanation free. What is ADP-ribosyl cyclase 2? Meaning of ADP-ribosyl cyclase 2 medical term. What does ADP-ribosyl cyclase 2 mean?
Accepted name: NAD+ glycohydrolase. Reaction: NAD+ + H2O = ADP-D-ribose + nicotinamide. Glossary: ADP-D-ribose = adenosine 5′-(5-deoxy-D-ribofuranos-5-yl diphosphate). Other name(s): NAD glycohydrolase; nicotinamide adenine dinucleotide glycohydrolase; β-NAD+ glycohydrolase; DPNase (ambiguous); NAD hydrolase (ambiguous); diphosphopyridine nucleosidase (ambiguous); nicotinamide adenine dinucleotide nucleosidase (ambiguous); NAD nucleosidase (ambiguous); DPN hydrolase (ambiguous); NADase (ambiguous); nga (gene name). Systematic name: NAD+ glycohydrolase. Comments: This enzyme catalyses the hydrolysis of NAD+, without associated ADP-ribosyl cyclase activities (unlike the metazoan enzyme EC 3.2.2.6, bifunctional ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase). The enzyme from Group A streptococci has been implicated in the pathogenesis of diseases such as streptococcal toxic shock-like syndrome (STSS) and necrotizing fasciitis. The enzyme from the venom of the snake Agkistrodon acutus also ...
Although activation of M2 muscarinic receptors is classically known to inhibit adenylyl cyclase activity (Peralta et al., 1988) or to modify membrane potential by inhibiting Ca2+-activated K+ channel (Kotlikoff et al., 1992), it has been recently proposed that M2 muscarinic receptors may induce Ca2+ signals by activation of the cADPR pathway (White et al., 2003) or by stimulation of a voltage-dependent Ca2+ channel (Cav1.2b) via the phosphatidylinositol 3-kinase/PKC pathway (Callaghan et al., 2004). Activation of the cADPR pathway by ACh in duodenum myocytes is shown by: (1) inhibition of Ca2+ oscillations by application of the cADPR competitive antagonist (8Br-cADPR), (2) inhibition of ACh-induced Ca2+ oscillations by inhibitors of ADP-ribosyl cyclase (ZnCl2, anti-CD38 antibody) and (3) detection of ADP-ribosyl cyclase activity by fluorescence experiments as the enzyme cyclizes NGD+ (non fluorescent) to produce cGDPR, a fluorescent compound (Graeff et al., 1994). This method has been used ...
A Membrane-bound or cytosolic enzyme that catalyzes the synthesis of Cyclic ADP-Ribose (cADPR) from Nicotinamide Adenine Dinucleotide (NAD). This enzyme generally catalyzes the Hydrolysis of cADPR to ADP-Ribose, as well, and sometimes the synthesis of Cyclic ADP-Ribose 2 phosphate (2-P-cADPR) from NADP ...
CD38 (NAD+ glycohydrolase) is a type II transmembrane glycoprotein able to induce activation, proliferation and differentiation of mature lymphocytes and mediate apoptosis of myeloid and lymphoid progenitor cells. Another role of CD38 is provided by enzymatic activity of its extracellular part. CD38 acts as NAD+ glycohydrolase converting NAD+ into ADP-ribose, as ADP-ribosyl cyclase producing cADPR and as cADPR hydrolase, thus affecting levels of calcium-mobilizing metabolites. ADPR produced by CD38 serves as an important second messenger of neutrophil and dendritic cell migration ...
CD38 is a 42-kilodalton glycoprotein expressed extensively on B and T lymphocytes. CD38 exhibits a structural homology to Aplysia adenosine diphosphate (ADP)-ribosyl cyclase. This enzyme catalyzes the synthesis of cyclic ADP-ribose (cADPR), a metabolite of nicotinamide adenine dinucleotide (NAD +) with calcium-mobilizing activity. A complementary DNA encoding the extracellular domain of murine CD38 was constructed and expressed, and the resultant recombinant soluble CD38 was purified to homogeneity. Soluble CD38 catalyzed the formation and hydrolysis of cADPR when added to NAD+. Purified cADPR augmented the proliferative response of activated murine B cells, potentially implicating the enzymatic activity of CD38 in lymphocyte function ...
The human lymphocyte antigen CD38 has been shown to share sequence homology with ADP-ribosyl cyclase, the enzyme that catalyzes the conversion of NAD+ to cyclic ADP-ribose (cADPR), a potent Ca(2+)-mobilizing agent. In this study COS1 cells from African Green Monkey kidney were transiently transfected with CD38 cDNA, inducing expression of authentic CD38 on the cell surface. We demonstrate that CD38 expressed in this manner can convert NAD+ to cADPR in the extracellular medium as assessed by Ca2+ release from sea-urchin egg microsomes.
ACROBiosystems provides a unique set of CD47-relevant products, including mutilple avi tag pre-biotinylated proteins, for rapid high throughput screening.
Cyclic adenosine diphosphate ribose (cADPR) is a potent endogenous calcium-mobilizing agent synthesized from beta-NAD+ by ADP-ribosyl cyclases in sea urchin eggs and in several mammalian cells (Galione, A., and White, A. (1994) Trends Cell Biol. 4, 431 436). Pharmacological studies suggest that cADPR is an endogenous modulator of Ca2+-induced Ca2+ release mediated by ryanodine-sensitive Ca2+ release channels. An unresolved question is whether cADPR can act as a Ca2+-mobilizing intracellular messenger. We show that exogenous application of nitric oxide (NO) mobilizes Ca2+ from intracellular stores in intact sea urchin eggs and that it releases Ca2+ and elevates cADPR levels in egg homogenates. 8-Amino-cADPR, a selective competitive antagonist of cADPR-mediated Ca2+ release, and nicotinamide, an inhibitor of ADP-ribosyl cyclase, inhibit the Ca2+-mobilizing actions of NO, while, heparin, a competitive antagonist of the inositol 1,4,5-trisphosphate receptor, did not affect NO-induced Ca2+ release. Since the
Clone REA465 recognizes the human CD157 antigen, a glycosylphosphatidylinositol (GPI) linked protein, which is also known as bone marrow stromal antigen 1 (BST-1) or cyclic ADP-ribose hydrolase 2. CD157, a member of the CD38 gene family, is an NAD-metabolizing ectoenzyme and a signaling molecule, which synthesizes the second messengers cyclic ADP-ribose and nicotinate-adenine dinucleotide phosphate, former a second messenger that elicits calcium release from intracellular stores. It is mainly expressed by cells of the myeloid lineage, bone marrow stroma, and vascular endothelium. CD157 is also expressed by ovarian cancer epithelium and by peritoneal mesothelial cells, where it is implicated in tumor dissemination. Further, it is endowed with receptor-like features observed in different cell types and transduces signals by interacting with transmembrane partner molecules, a strategy shared by other GPI-anchored molecules. CD157 is involved in neutrophil polarization, adhesion, and motility and controls
Chemical-registry-number] 0 / Antibodies, Neoplasm; 0 / Antigens, CD; 0 / Antigens, CD7; 0 / Antigens, Differentiation; 0 / Antineoplastic Agents, Phytogenic; 0 / Carrier Proteins; 0 / Immunoglobulin G; 0 / Immunotoxins; 0 / Lipoproteins, HDL; 0 / Membrane Glycoproteins; 0 / Plant Proteins; 0 / RNA-Binding Proteins; 0 / Receptors, Lipoprotein; 0 / Ribosome Inactivating Proteins, Type 1; 0 / high density lipoprotein receptors; 147605-06-9 / high density lipoprotein binding protein; EC 3.2.2.- / N-Glycosyl Hydrolases; EC 3.2.2.22 / saporin; EC 3.2.2.5 / ADP-ribosyl Cyclase; EC 3.2.2.5 / Antigens, CD38; EC 3.2.2.5 / Cd38 protein, mouse; EC 3.2.2.5 / NAD+ ...
Poly(ADP-ribosyl)ation is a post-translational modification of proteins involved in regulation of many cellular pathways. Poly(ADP-ribose) (PAR) consists of chains of repeating ADP-ribose nucleotide units and is synthesized by the family of enzymes called poly(ADP-ribose) polymerases (PARPs). This modification can be removed by the hydrolytic action of poly(ADP-ribose) glycohydrolase (PARG) and ADP-ribosylhydrolase 3 (ARH3). Hydrolytic activity of macrodomain proteins (MacroD1, MacroD2 and TARG1) is responsible for the removal of terminal ADP-ribose unit and for complete reversion of protein ADP-ribosylation. Poly(ADP-ribosyl)ation is widely utilized in eukaryotes and PARPs are present in representatives from all six major eukaryotic supergroups, with only a small number of eukaryotic species that do not possess PARP genes. The last common ancestor of all eukaryotes possessed at least five types of PARP proteins that include both mono and poly(ADP-ribosyl) transferases. Distribution of PARGs strictly
Cyclic ADP-ribose (cADPR) is a putative second messenger that has been demonstrated to mobilize Ca2+ in many cell types. Its postulated role as the endogenous regulator of ryanodine-sensitive Ca2+ release channels has been greatly supported by the advent and use of specific cADPR receptor antagonists such as 8-NH2-cADPR (Walseth, T. F., and Lee, H. C. (1993) Biochim. Biophys. Acta 1178, 235-242). However, investigations of the role of cADPR in physiological responses, such as fertilization, stimulus-secretion coupling, and excitation-contraction coupling, have been hindered by the susceptibility of cADPR receptor antagonists to hydrolysis and the need to introduce these molecules into cells by microinjection or patch clamp techniques. We have recently reported on the discovery of a poorly hydrolyzable analogue of cADPR, 7-deaza-cADPR (Bailey, V. C., Sethi, J. K., Fortt, S. M., Galione, A., and Potter, B. V. L. (1997) Chem. Biol. 4, 41-51) but this, like cADPR, is an agonist of ryanodine-sensitive Ca2+
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Clone REA976 recognizes the human CD370 antigen, also known as C-type lectin domain family 9 member A (CLEC9A). CD370 is a type II transmembrane glycoprotein member of 241 amino acids with a predicted molecular mass of ~30 kDa. It is also known as DNGR-1 and is expressed on BDCA-3+ myeloid dendritic cells from peripheral blood and lymphoid tissues. CD370 acts as a receptor for necrotic cells and plays an important role in cross-presentation. Additional information: Clone REA976 displays negligible binding to Fc receptors. - Great Britain
PE anti-human CD13 Antibody - CD13 is a 150-170 kD type II transmembrane glycoprotein also known as aminopeptidase N, APN, and gp150.
Cadp-ribose/ACM119340535 can be provided in Alfa Chemistry. We are dedicated to provide our customers the best products and services.
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
Background: Poly (ADP-ribosyl) polymerase 1 (PARP1) is important in maintaining genomic stability, repairing DNA damage, and regulating transcriptional processe
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MarketResearchReports.biz has recently announced the addition of a market study Tumor Necrosis Factor Receptor Superfamily Member 5 (B Cell Surface Antigen CD40 or Bp50 or CDw40 or CD40L Receptor or TNFRSF5 or CD40) - Pipeline Review, H2 2016 , is a comparative analysis of the global market.. Tumor Necrosis Factor Receptor Superfamily Member 5 (B Cell Surface Antigen CD40 or Bp50 or CDw40 or CD40L Receptor or TNFRSF5 or CD40) - Pipeline Review, H2 2016. Summary. Global Markets Directs, Tumor Necrosis Factor Receptor Superfamily Member 5 (B Cell Surface Antigen CD40 or Bp50 or CDw40 or CD40L Receptor or TNFRSF5 or CD40) - Pipeline Review, H2 2016, provides in depth analysis on Tumor Necrosis Factor Receptor Superfamily Member 5 (B Cell Surface Antigen CD40 or Bp50 or CDw40 or CD40L Receptor or TNFRSF5 or CD40) targeted pipeline therapeutics.. The report provides comprehensive information on the Tumor Necrosis Factor Receptor Superfamily Member 5 (B Cell Surface Antigen CD40 or Bp50 or CDw40 or ...
Nicotinic acid adenine dinucleotide phosphate (NAADP) is the most potent Ca(2+)-mobilizing intracellular messenger and is linked to a variety of stimuli and cell surface receptors. However, the enzyme responsible for endogenous NAADP synthesis in vivo is unknown, and it has been proposed that another enzyme differing from ADP-ribosyl cyclase family members may exist. The ecto-enzyme CD38, involved in many functions as diverse as cell proliferation and social behavior, represents an important alternative. In pancreatic acinar cells, the hormone cholecystokinin (CCK) stimulates NAADP production evoking Ca(2+) signals by discharging acidic Ca(2+) stores and leading to digestive enzyme secretion. From cells derived from CD38(-/-) mice, we provide the first physiological evidence that CD38 is required for endogenous NAADP generation in response to CCK stimulation. Furthermore, CD38 expression in CD38-deficient pancreatic AR42J cells remodels Ca(2+)-signaling pathways in these cells by restoring Ca(2+)
BACKGROUND AND PURPOSE: Recently, a number of mimics of the second messenger cyclic ADP-ribose (cADPR) with replacement of adenosine by inosine were introduced. In addition, various alterations in the molecule ranging from substitutions at C8 of the base up to full replacement of the ribose moieties still retained biological activity. However, nothing is known about the metabolic stability and cellular effects of these novel analogues. EXPERIMENTAL APPROACH: cADPR and the inosine-based analogues were incubated with CD38, ADP-ribosyl cyclase and NAD-glycohydrolase and metabolism was analysed by RP-HPLC. Furthermore, the effect of the analogues on cytokine expression and proliferation was investigated in primary T-lymphocytes and T-lymphoma cells. KEY RESULTS: Incubation of cADPR with CD38 resulted in degradation to adenosine diphosphoribose. ADP-ribosyl cyclase weakly catabolised cADPR whereas NAD-glycohydrolase showed no such activity. In contrast, N1-cyclic inosine 5-diphosphoribose (N1-cIDPR) was not
NAADP (nicotinic acid-adenine dinucleotide phosphate), the most potent Ca2+-mobilizing second messenger, is active in a wide range of organisms and cell types. Until now, all NAADP-producing enzymes have been thought to be members of the ADP-ribosyl cyclase family. ADP-ribosyl cyclases exhibit promiscuous substrate selectivity, synthesize a variety of products and are regulated in a limited manner, which may be non-physiological. In the present paper, we report the presence of an enzyme on the surface of sea urchin sperm that exhibits bell-shaped regulation by Ca2+ over a range (EC(50) of 10 nM and IC(50) of 50 microM) that is physiologically relevant. Uniquely, this surface enzyme possesses complete selectivity for nucleotides with a 2-phosphate group and exhibits only base-exchange activity without any detectable cyclase activity. Taken together, these findings indicate that this novel enzyme should be considered as the first true NAADP synthase.
TY - JOUR. T1 - Drosophila forkhead homologues are expressed in CD34+HLA-DR- primitive human hematopoietic progenitors. AU - Hromas, Robert. AU - Klemsz, Michael. AU - Amaravadi, Lakshmi. AU - Hufford, Tricia. AU - Huang, Irene. AU - Desai, Alpana. AU - Srour, Edward. AU - Bruno, Edward. AU - Hoffman, Ronald. PY - 1994. Y1 - 1994. N2 - The Forkhead gene (FKH) regulates morphogenesis in Drosophila. It is the prototype of a new family of transcriptional activators. We used the polymerase chain reaction (PCR) to analyze the expression pattern of this new transcriptional regulatory gene family in primitive hematopoeitic progenitors. Partially degenerate oligonucleotides to two conserved amino acid sequences of this family were used to prime a PCR amplification of cDNA synthesized from CD34+HLA-DR- hematopoietic cells. Known and novel FKH genes were found to be expressed in these cells.. AB - The Forkhead gene (FKH) regulates morphogenesis in Drosophila. It is the prototype of a new family of ...
Cyclic ADP-ribose (cADPR) and nicotinic acid adenine dinucleotide phosphate (NAADP) mobilize Ca2+ from two different types of intracellular stores and through completely independent mechanisms. The two Ca2+ messengers are also structurally distinct. cADPR is a cyclic nucleotide derived from NAD, whi …
Cyclic ADP-ribose and hydrogen peroxide synergize with ADP-ribose in the activation of TRPM2 channels. Mol Cell. 2005 Apr 1;18(1):61-9. PMED ID: 15808509. ...
Abstract. Evidence has been provided recently that shows that high concentrations of cytokines can fulfill functions previously attributed to stromal cells, su
productone is a polyclonal antibody of high purity and binding affinity for the antigen that it is risen against. Properly used, this antibody will ensure excellent and reproducible results with guaranteed success for the applications that it is tested in. Polyclonal antibodies have series of advantages - larger batches can be supplied at a time, they are inexpensive to manufacture and respectively to buy, the time needed for production is considerably shorter. Polyclonal antibodies generally are more stable and retain their reactivity under unfavorable conditions. To obtain more detailed information on productone, please, refer to the full product datasheet ...
This multiunctional enzyme catalyses both the synthesis and hydrolysis of cyclic ADP-ribose, a calcium messenger that can mobilize intracellular Ca2+ stores and activate Ca2+ influx to regulate a wide range of physiological processes. In addition, the enzyme also catalyses EC 2.4.99.20, 2-phospho-ADP-ribosyl cyclase/2-phospho-cyclic-ADP-ribose transferase. cf. EC 3.2.2.5, NAD+ glycohydrolase ...
A bifunctional enzyme that catalyzes the synthesis and HYDROLYSIS of CYCLIC ADP-RIBOSE (cADPR) from NAD+ to ADP-RIBOSE. It is a cell surface molecule which is predominantly expressed on LYMPHOID CELLS and MYELOID CELLS ...
Felhívjuk felhasználóink figyelmét arra, hogy a DEA Egyetemi IP és Könyvtári számítógépek elérési szintű dokumentumai kizárólag oktatási, kutatási, valamint saját tanulási célokra használhatóak fel, azt nem oszthatják meg az interneten és nem terjeszthetik. A dokumentum és a pdf megjelenítő védelmének megkerülése (másolás, nyomtatás, letöltés korlátozása) tilos ...
Increasing evidence has indicated that NAD+ and NADH play critical roles not only in energy metabolism, but also in cell death and various cellular functions including regulation of calcium homeostasis and gene expression. It has also been indicated that NAD+ and NADH are mediators of multiple major biological processes including aging. NAD+ and NADH produce the biological effects by regulating numerous NAD+/NADH-dependent enzymes, including dehydrogenases, poly(ADP-ribose) polymerases, Sir2 family proteins (sirtuins), mono(ADP-ribosyl)transferases, and ADP-ribosyl cyclases. Of particular interest, NAD+-dependent generation of ADP-ribose, cyclic ADP-ribose and O-acetyl-ADP-ribose can mediate calcium homeostasis by affecting TRPM2 receptors and ryanodine receptors; and sirtuins and PARPs appear to play key roles in aging, cell death and a variety of cellular functions. It has also been indicated that NADH and NAD+ can be transported across plasma membranes of cells, and that extracellular NAD+ ...
Purified anti-human CD70 Antibody - CD70, also known as CD27L, is a 50 kD type II transmembrane glycoprotein and member of the tumor necrosis factor superfamily.
This gene encodes a type II transmembrane glycoprotein that is the highly polymorphic Kell blood group antigen. The Kell glycoprotein links via a single disulfide bond to the XK membrane protein that carries the Kx antigen. The encoded protein contains sequence and structural similarity to members of the neprilysin (M13) family of zinc endopeptidases ...
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a type II transmembrane glycoprotein that is the highly polymorphic Kell blood group antigen. The Kell glycoprotein links via a single disulfide bond to the XK membrane protein that carries the Kx antigen. The encoded protein contains sequence and structural similarity to members of the neprilysin (M13) family of zinc endopeptidases. [provided by RefSeq, Jul 2008 ...
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008 ...
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TY - JOUR. T1 - Aberrant cyclization affords a C-6 modified cyclic adenosine 5′-diphosphoribose analogue with biological activity in Jurkat T cells. AU - Moreau, Christelle. AU - Kirchberger, Tanja. AU - Zhang, Bo. AU - Thomas, Mark P.. AU - Weber, Karin. AU - Guse, Andreas H.. AU - Potter, Barry V. L.. PY - 2012. Y1 - 2012. N2 - Two nicotinamide adenine dinucleotide (NAD +) analogues modified at the 6 position of the purine ring were synthesized, and their substrate properties toward Aplysia californica ADP-ribosyl cyclase were investigated. 6-N-Methyl NAD + (6-N-methyl nicotinamide adenosine 5′-dinucleotide 10) hydrolyzes to give the linear 6-N-methyl ADPR (adenosine 5′-diphosphoribose, 11), whereas 6-thio NHD + (nicotinamide 6-mercaptopurine 5′-dinucleotide, 17) generates a cyclic dinucleotide. Surprisingly, NMR correlation spectra confirm this compound to be the N1 cyclic product 6-thio N1-cIDPR (6-thio cyclic inosine 5′-diphosphoribose, 3), although the corresponding 6-oxo ...
The only curative therapy for sickle cell disease (SCD) is allogeneic hematopoietic stem cell (HSC) transplantation. Gene therapy approaches for autologous HSC transplantation are being developed. Although earlier engraftment is seen when cells from GCSF-mobilized blood are transplanted than when bone marrow is transplanted, administration of GCSF to patients with SCD can cause significant morbidity. We tested whether primitive hematopoietic progenitors are spontaneously mobilized in the blood of patients with SCD during acute crisis (AC-SCD patients). The frequency of myeloid-lymphoid-initiating cells (ML-ICs) and SCID-repopulating cells (SRCs) was significantly higher in blood from AC-SCD patients than in blood from patients with steady-state SCD or from normal donors. The presence of SRCs in peripheral blood was not associated with detection of long-term culture-initiating cells, consistent with the notion that SRCs are more primitive than long-term culture-initiating cells. As ML-ICs and ...
Since tumor necrosis factor (TNF)-alpha, interferon (IFN)-gamma, and transforming growth factor (TGF)-beta have all been shown to be specific inhibitors of early human hematopoiesis, we wanted to investigate the interactions of these three cytokines on very primitive human adult bone marrow CD34++CD38- hematopoietic progenitor cells, using a pre-colony-forming cell (pre-CFC) assay, which detects the effects of these cytokines on the initial phases of the differentiation of these primitive progenitors, which are unresponsive to interleukin (IL) 3 alone. Surprisingly, TNF-alpha was a very potent stimulator of the proliferation of CD34++CD38- cells and was the most potent synergistic factor for the IL-3-induced proliferation of these cells of all cytokines tested (IL-1, IL-6, granulocyte colony-stimulating factor, kit ligand). TNF-alpha was the only cytokine that, as a single added factor, induced substantial proliferation in CD34++CD38- cells in the presence of IL-3, except for kit ligand, which ...
Hypothalamic oxytocin (OT) is released into the brain by cyclic ADP-ribose (cADPR) with or without depolarizing stimulation. Previously, we showed that the intracellular free calcium concentration ([Ca2+]i) that seems to trigger OT release can be elevated by -NAD+, cADPR, and ADP in mouse oxytocinergic neurons. As these -NAD+ metabolites activate warm-sensitive TRPM2 cation channels, when the incubation temperature is increased, the [Ca2+]i in hypothalamic neurons is elevated. However, it has not been determined whether OT release is facilitated by heat in vitro or hyperthermia in vivo in combination with cADPR. Furthermore, it has not been examined whether CD38 and TRPM2 exert their functions on OT release during stress or stress-induced hyperthermia in relation to the anxiolytic roles and social behaviors of OT under stress conditions. Here, we report that OT release from the isolated hypothalami of male mice in culture was enhanced by extracellular application of cADPR or increasing the
Nicotinamide adenine dinucleotide (NAD+) is the universal currency of energy metabolism and electron transfer. Recent studies indicate that apart from its role as a coenzyme, NAD+ and its metabolites also function in cell signaling pathways; for example, they are substrates for nucleotide-metabolizing enzymes and ligands for extra- and intracellular receptors and ion channels. Moreover, the NAD+ and NAD+ phosphate metabolites adenosine 5′-diphosphoribose (ADP-ribose), cyclic ADP-ribose, and nicotinic acid adenine dinucleotide phosphate (NAADP) have emerged as key second messengers in Ca2+ signaling. A symposium in Hamburg, Germany, brought together 120 researchers from various fields, who were all engaged in the molecular characterization of the key players of NAD+ signaling (www.NAD2008.de).. ...
Ligation of the T-cell receptor/CD3 complex results in global Ca(2+) signals that are essential for T-cell activation. We have recently reported that these global Ca(2+) signals are preceded by localized pacemaker Ca(2+) signals. Here, we demonstrate for the first time for human T cells that an increase in signal frequency of subcellular pacemaker Ca(2+) signals at sites close to the plasma membrane, in the cytosol and in the nucleus depends on the type 3 ryanodine receptor (RyR) and its modulation by cyclic ADP-ribose. The spatial distribution of D-myo-inositol 1,4,5-trisphosphate receptors and RyRs indicates a concerted action of both of these receptors/Ca(2+) channels in the generation of initial pacemaker signals localized close to the plasma membrane. Inhibition or knockdown of RyRs resulted in significant decreases in (1) the frequency of initial pacemaker signals localized close to the plasma membrane, and (2) the frequency of localized pacemaker Ca(2+) signals in the inner cytosol. Moreover,
Ofatumumab (oh fa toom ue mab) is a human monoclonal IgG1 antibody to the cell surface antigen CD20 (also known as human B lymphocyte restricted differentiation antigen: Bp35), which is found on mature B cells as well as 90% of neoplastic B cell such as occur in chronic lymphocytic leukemia. CD20 is not present on pro-B cells, hematopoietic stem cells, normal plasma cells or other normal lymphocytes, circulating cells or tissues. Engagement of ofatumumab with CD20 leads to B cell lysis and depletion of circulating and tissue B cells for an extended period, up to 6 to 8 months. There is an accompanying mild decrease in IgM, but no change in IgG or IgA levels. ...
Description: This gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. This gene, which encodes a 100-kD type II transmembrane glycoprotein, exists in a single copy of greater than 45 kb. The 5 untranslated region of this gene is alternatively spliced, resulting in four separate mRNA transcripts. The coding region is ...
Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. This gene, which encodes a 100-kD type II transmembrane glycoprotein, exists in a single copy of greater than 45 kb. The 5' untranslated region of this gene is ...
FUNCTION: This gene encodes a member of the nucleoside pyrophosphatase/phosphodiesterase family of enzymes that catalyzes the hydrolysis of pyrophosphate and phosphodiester bonds in nucleotide triphosphates and oligonucleotides, respectively, to generate nucleoside 5'-monophosphates. The encoded protein is a type II transmembrane glycoprotein that negatively regulates bone mineralization. Mice harboring a nonsense mutation in this gene, termed tiptoe walking (ttw), exhibit ectopic ossification of the spinal ligaments. The encoded protein binds to the insulin receptor, inhibits downstream signaling events and induces insulin resistance and glucose tolerance. This gene is located adjacent to a paralog on chromosome 10. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015 ...
CD20 is a B cell-specific 35/37 kDa integral membrane protein which modulates proliferation and differentiation of normal resting B cells when stimulated by CD20 antibodies. An increase in c-myc mRNA levels occurs within hours after treatment of resting B cells with CD20 mAb; however earlier events in the CD20 signal transduction pathway have not been described. Here we demonstrate that anti-CD20 mediated induction of c-myc mRNA is inhibited by the tyrosine kinase inhibitor herbimycin A, that CD20 is associated with both tyrosine and serine kinase activity, and that tyrosine phosphorylation of multiple substrates is induced within minutes upon ligation of CD20 with mAb. Association of the tyrosine and serine kinases with CD20 was stable in lysis buffer containing 1% NP40 and 0.25% deoxycholate. Under the same conditions, antibodies against several other B cell surface molecules failed to co-precipitate tyrosine kinase activity, however, a serine kinase was precipitated by the anti-CD19 mAb, B43. ...
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... whereas its two products are ADP-ribose and nicotinamide. Unlike ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase (EC 3.2.2.6), ... "ENZYME - 3.2.2.6 ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase". enzyme.expasy.org. Retrieved 2022-07-11. Hofmann EC, ... "Crystallographic studies on human BST-1/CD157 with ADP-ribosyl cyclase and NAD glycohydrolase activities". Journal of Molecular ... which catalyzes the same reaction, this reaction does not proceed through a cyclic ADP-ribose. This enzyme belongs to the ...
January 1998). "Human CD38 (ADP-ribosyl cyclase) is a counter-receptor of CD31, an Ig superfamily member". Journal of ...
Overview of all the structural information available in the PDB for UniProt: Q10588 (Human ADP-ribosyl cyclase/cyclic ADP- ... Bst1 (Bone marrow stromal cell antigen 1, ADP-ribosyl cyclase 2, CD157) is an enzyme that in humans is encoded by the BST1 gene ... CD157 and CD38 are both members of the ADP-ribosyl cyclase family of enzymes that catalyze the formation of nicotinamide and ... 2002). "Crystallographic studies on human BST-1/CD157 with ADP-ribosyl cyclase and NAD glycohydrolase activities". J. Mol. Biol ...
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1) at the PDBe-KB. (Articles with short description, Short description matches ... ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase), to chromosome 4p15". Cytogenetics and Cell Genetics. 69 (1-2): 38-9. doi: ... ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase): organization, nucleotide sequence and alternative splicing". Gene. 186 (2): ... ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase): organization, nucleotide sequence and alternative splicing". Gene. 186 (2): ...
... catalyzed by ADP-ribosyl cyclases) which are a family of enzymes that include CD38 and CD157 in mammals (and orthologs in sea ... because genetic knockout or knock-down of ADP-ribosyl cyclases has no effect on NAADP production in some cell types), and there ... "Acidic residues at the active sites of CD38 and ADP-ribosyl cyclase determine nicotinic acid adenine dinucleotide phosphate ( ... Lee HC, Zhao YJ (2019). "Resolving the topological enigma in Ca 2+ signaling by cyclic ADP-ribose and NAADP". Journal of ...
Toxins that interfere with enzymes ADP-ribosyl-transferases, and invasive adenylate cyclases. Cholera toxin is an AB toxin that ... Adenylate cyclase is activated by a range of signaling molecules through the activation of adenylate cyclase stimulatory G (Gs ... Adenylate cyclase is inhibited by agonists of adenylate cyclase inhibitory G (Gi)-protein-coupled receptors. Liver adenylate ... This occurs through inhibition of the cAMP-producing enzyme, adenylate cyclase, as a side-effect of glucose transport into the ...
ADP-ribosyl cyclase allows for synthesis from nicotinamide in the salvage pathway, and NADP+ phosphatase can convert NADPH back ...
... adp-ribosyl cyclase MeSH D08.811.913.400.725.115.680 - pertussis toxin MeSH D08.811.913.400.725.115.690 - poly(adp-ribose) ... adp-ribosyl cyclase MeSH D08.811.277.450.737.400.060.500 - antigens, cd38 MeSH D08.811.277.450.770 - oligo-1,6-glucosidase MeSH ... adenylate cyclase MeSH D08.811.520.650.200.040 - adenylate cyclase toxin MeSH D08.811.520.650.600 - guanylate cyclase MeSH ... adp-ribosylation factors MeSH D08.811.277.040.330.300.400.100.100 - ADP-ribosylation factor 1 MeSH D08.811.277.040.330.300. ...
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase EC 3.2.2.7: adenosine nucleosidase EC 3.2.2.8: ribosylpyrimidine nucleosidase ... ADP-ribosyl-[dinitrogen reductase] hydrolase EC 3.2.2.25: N-methyl nucleosidase EC 3.2.2.26: futalosine hydrolase EC 3.2.2.27: ... ADP-dependent short-chain-acyl-CoA hydrolase EC 3.1.2.19: ADP-dependent medium-chain-acyl-CoA hydrolase EC 3.1.2.20: acyl-CoA ... ADP-phosphoglycerate phosphatase EC 3.1.3.29: N-acylneuraminate-9-phosphatase EC 3.1.3.30: The activity may be that of an acid ...
During the late 1980s, ADP-ribosyl cyclases, which catalyze the addition of cyclic-ADP-ribose groups to proteins, were ... ADP-ribosyl)transferases can perform two types of modifications: mono(ADP-ribosyl)ation and poly(ADP-ribosyl)ation. Mono(ADP- ... ADP-ribosyl)ation and short poly(ADP-ribosyl)ation and serves to activate PARP1. The PARPs have many protein targets at the ... ADP-ribosyl)transferase, has been shown to affect STAT transcription factor binding. Other (ADP-ribosyl)transferases have been ...
Prasad GS, McRee DE, Stura EA, Levitt DG, Lee HC, Stout CD (1996). "Crystal structure of Aplysia ADP-ribosyl cyclase, a homolog ... cADPR is produced from nicotinamide adenine dinucleotide (NAD+) by ADP-ribosyl cyclases (EC 3.2.2.5) as part of a second ... monofunctional ADP ribosyl cyclase of the mollusc Aplysia). The same enzymes are also capable of hydrolyzing cADPR to ADPR. ... Hon Cheung Lee, the discoverer of cyclic ADP-ribose. Cyclic ADP-ribose and NAADP. The first book on these two second messengers ...
In enzymology, a ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase (EC 3.2.2.6) is a bifunctional enzyme that catalyzes the ... "ENZYME - 3.2.2.6 ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase". enzyme.expasy.org. Retrieved 2022-07-11. ALIVISATOS SG, ... The reaction proceeds through cyclic ADP-ribose (cADPR) as intermediate, which is then hydrolyzed into ADP-ribose. This makes ... ADP-ribose + nicotinamide The 3 substrates of this enzyme are NAD+ and H2O, whereas its two products are ADP-ribose and ...
5-phospho-β-D-ribosyl)acetamidine ⇌ {\displaystyle \rightleftharpoons } ADP + 5-amino-1-(5-phospho-β-D-ribosyl)imidazole + ... It is a sequential mechanism in which ATP binds first to the enzyme and ADP is released last. This enzyme hydrolyzes ATP to ... The systematic name of this enzyme class is 2-(formamido)-N1-(5-phosphoribosyl)acetamidine cyclo-ligase (ADP-forming). Other ... ADP-forming), phosphoribosylaminoimidazole synthetase, and phosphoribosylformylglycinamidine cyclo-ligase. Purines are one of ...
... phospho-ADP-ribosyl cyclase/2′-phospho-cyclic-ADP-ribose transferase (*) EC 2.4.99.21: dolichyl-phosphooligosaccharide-protein ... ADP-dependent phosphofructokinase EC 2.7.1.147: ADP-dependent glucokinase EC 2.7.1.148: 4-(cytidine 5′-diphospho)-2-C-methyl-D- ... NAD+ ADP-ribosyltransferase EC 2.4.2.31: NAD+-protein-arginine ADP-ribosyltransferase EC 2.4.2.32: dolichyl-phosphate D- ... NAD+-diphthamide ADP-ribosyltransferase EC 2.4.2.37: NAD+ -dinitrogen-reductase ADP-D-ribosyltransferase EC 2.4.2.38: ...
... which is produced from NAD+ by ADP-ribosyl cyclases, as part of a second messenger system. This molecule acts in calcium ... Poly(ADP-ribosyl)ation is carried out by the poly(ADP-ribose) polymerases. The poly(ADP-ribose) structure is involved in the ... or the transferral of ADP-ribose to proteins in long branched chains, which is called poly(ADP-ribosyl)ation. Mono-ADP- ... ADP-ribosylation involves either the addition of a single ADP-ribose moiety, in mono-ADP-ribosylation, ...
fGAR + L-Glutamine + ATP → fGAM + L-Glutamate + ADP + Pi The fifth is catalyzed by AIR synthetase (FGAM cyclase). fGAM + ATP → ... A key regulatory step is the production of 5-phospho-α-D-ribosyl 1-pyrophosphate (PRPP) by ribose phosphate pyrophosphokinase, ... PRPP + L-Glutamine + H2O → PRA + L-Glutamate + PPi In the second step react PRA, glycine and ATP to create GAR, ADP, and ... CAIR + L-Aspartate + ATP → SAICAR + ADP + Pi The eight is catalyzed by adenylosuccinate lyase. SAICAR → AICAR + Fumarate The ...
Mass spectrometry analysis revealed that SidJ is a glutamylase that modifies the catalytic glutamate in the mono-ADP ribosyl ... They also established the tightly light-regulated guanylyl-cyclase opsin CyclOp that enabled rapid light-triggered cGMP ... In addition, they studied synaptic transmission after photostimulation, using ChR2 and a photoactivated adenylyl cyclase (PAC ... "Optogenetic manipulation of cGMP in cells and animals by the tightly light-regulated guanylyl-cyclase opsin CyclOp". Nat Commun ...

No data available that match "adp ribosyl cyclase"


ADP-ribosyl Cyclase 1. Antigens, CD4. CD4 Antigens. Antigens, CD40. CD40 Antigens. ...
3I9L: Crystal structure of ADP ribosyl cyclase complexed with N1-cIDPR Liu Q., Graeff R., Kriksunov IA., Jiang H., Zhang B., ...
Identification and localisation of novel ADP-ribosyl cyclases in sea urchin eggs ... Identification and localisation of novel ADP-ribosyl cyclases in sea urchin eggs ...
CD38/ADP-ribosyl cyclase in the rat sublingual gland: Subcellular localization under resting and saliva-secreting conditions. ... CD38/ADP-ribosyl cyclase in the rat sublingual gland : Subcellular localization under resting and saliva-secreting conditions. ... CD38/ADP-ribosyl cyclase in the rat sublingual gland : Subcellular localization under resting and saliva-secreting conditions. ... T1 - CD38/ADP-ribosyl cyclase in the rat sublingual gland. T2 - Subcellular localization under resting and saliva-secreting ...
ADP ribosyl cyclase 1 antibody. *ADP ribosyl cyclase antibody. *ADP ribosyl cyclase/cyclic ADP-ribose hydrolase antibody ... Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. ...
MeSH Terms: ADP-ribosyl Cyclase 1/deficiency*; ADP-ribosyl Cyclase 1/metabolism; Administration, Inhalation; Allergens/ ...
ADP-Ribosyl Cyclase as a Therapeutic Target for Central Nervous System Diseases. Journal: Central Nervous System Agents in ... Poly (ADP-Ribosyl) Polymerase 1 Inhibitors: A Patent Review. Journal: Recent Patents on Anti-Cancer Drug Discovery. Volume: 12 ... Poly(ADP-Ribosyl)ation Enzyme-1 as a Target for Neuroprotection in Acute Central Nervous System Injury. Journal: Current Drug ... ADP-Ribosyltransferases and Poly ADP-Ribosylation. Journal: Current Protein & Peptide Science. Volume: 16 Issue: 6 Year: 2015 ...
ADP-ribosyl Cyclase / genetics* Actions. * Search in PubMed * Search in MeSH * Add to Search ...
ADP-ribosyl Cyclase 1 / immunology* Actions. * Search in PubMed * Search in MeSH ...
... phospho-cyclic-ADP-ribose transferase; ADP-ribosyl cyclase 1; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; ADPRC 1; cADPr ... Protein Aliases: 2-phospho-ADP-ribosyl cyclase; 2-phospho-ADP-ribosyl cyclase/2-phospho-cyclic-ADP-ribose transferase; 2- ... CD38 functions as a multi-catalytic ectoenzyme serving as ADP-ribosyl cyclase, cyclic ADP-ribose hydrolase and possibly NAD+ ... CD38, a counter-receptor for CD31, is an ectoenzyme with cyclase and hydrolase enzymatic activity and is speculated to play a ...
It is an ectoenzyme that has both cyclic ADP-ribose hydrolase and ADP-ribosyl cyclase activities. CD157 is expressed as a ... It is an ectoenzyme that has both cyclic ADP-ribose hydrolase and ADP-ribosyl cyclase activities. CD157 is expressed as a ... ADP-ribosyl cyclase 2, and cADPr hydrolase 2. CD157 is a 40-46 kDa glycophosphatidylinositol-linked cell membrane glycoprotein ... ADP-ribosyl cyclase 2, and cADPr hydrolase 2. CD157 is a 40-46 kDa glycophosphatidylinositol-linked cell membrane glycoprotein ...
T10, ADP-ribosyl cyclase Isotype Mouse IgG1, κ Ave. Rating Submit a Review Product Citations publications Human peripheral ... Ecto-ADP-ribosyl cyclase, calcium signaling, cell activation Ligand/Receptor CD31, hyaluronic acid Cell Type B cells, Dendritic ... ADP-ribosyl cyclase, ectoenzyme, type II glycoprotein, 45 kD Distribution T cells, B cells, NK, myeloid, plasma, and dendritic ... It is an ADP-ribosyl hydrolase expressed at variable levels on hematopoietic cells and in some non-hematopoietic tissues (such ...
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2 Show on y-axis - ...
ADP-ribosyl cyclase/Cyclic ADP-ribose hydrolase)to chromosome 4p15. Cytogenet.Cell Genet.69. 38-39 (1995). *. Related Report. ... Cloning and characterization of cDNA encoding rat ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase(homologue to human CD38)from ... Publications] Y.Hirata,Y.Ohsugi,H.Okamoto,T.Kaisho,T.Hirano,et all.: ADP ribosyl cyclase activity of a novel bone marrow ... The structure of the Aplysia kurodai gene encoding ADP-ribosyl cyclase,a secondmessenger enzyme. Gene.(in press).. *. Related ...
ADP-ribosyl cyclase, T10. Molecular mass of antigen [kDa]. 34. Distribution of antigen. B cells, monocytes, NK cells, T cells, ...
Evolution and function of the ADP ribosyl cyclase/CD38 gene family in physiology and pathology. Physiol Rev 88: 841-886. ... Evolution and function of the ADP ribosyl cyclase/CD38 gene family in physiology and pathology. Physiol Rev 88: 841-886.) and ...
CD38 , ADP-ribosyl cyclase 1 , CD38 antigen (p45) , CD38 antigen , Cyclic ADP-ribose hydrolase 1 , T10 , CADPr hydrolase 1 , ...
It has also been reported that T3 may induce the activation of ADP-ribosyl cyclase, which promotes Ca2+ release and therefore ...
Yamamoto-Katayama, S. et al., Crystallographic studies on human BST-1/CD157 with ADP-ribosyl cyclase and NAD glycohydrolase ...
cADPR is a Ca 2 -modulating second messenger formed by the cyclisation of NAD+ by ADP-ribosyl cyclases. While three different ... In addition; NAD is a precursor to biologically active metabolites such as ADPR, ADP_ribosyl-derivatives and cADPR. ... The aim of this work is thus to synthesise base-modified NAD+ analogues that can reach the cyclase active site, and be ... Such analogues may facilitate establishing the location and mechanism of the cyclase and also the metabolic pathway of cADPR. ...
ADP-ribosyl Cyclase [D08.811.277.450.430.400.060] * ADP-ribosyl Cyclase 1 [D08.811.277.450.430.400.060.500] ... 2-Phospho-adenosine Diphosphate Ribose Cyclase ADP Ribose Cyclase ADPR Cyclase Adenosine Diphosphate Ribose Cyclase Cyclic ADP ... ADP Ribose Transferases [D08.811.913.400.725.115] * NAD+ Nucleosidase [D08.811.913.400.725.115.660] * ADP-ribosyl Cyclase [ ... and sometimes the synthesis of cyclic ADP-ribose 2 phosphate (2-P-cADPR) from NADP.. Terms. ADP-ribosyl Cyclase Preferred ...
Identification of ADP-ribosyl cyclase in Arabidopsis. Tanvir Shams Qureshi. Department of Civil Engineering, Leading University ...
Deaglio S, Morra M, Mallone R. Human CD38 (ADP-ribosyl cyclase) is a counter-receptor of CD31, an Ig superfamily member. J ...
ADP-ribosyl cyclase 1; NAD(+) nucleosidase. CD39. ENTPD1. CD39. ATPDase; CD39; NTPDase-1. ...
ADP-ribosyl Cyclase 1 Medicine & Life Sciences 19% * CD34 Antigens Medicine & Life Sciences 17% ...
ADP-ribosyl Cyclase [D08.811.277.450.430.400.060] * ADP-ribosyl Cyclase 1 [D08.811.277.450.430.400.060.500] ... 2-Phospho-adenosine Diphosphate Ribose Cyclase ADP Ribose Cyclase ADPR Cyclase Adenosine Diphosphate Ribose Cyclase Cyclic ADP ... ADP Ribose Transferases [D08.811.913.400.725.115] * NAD+ Nucleosidase [D08.811.913.400.725.115.660] * ADP-ribosyl Cyclase [ ... and sometimes the synthesis of cyclic ADP-ribose 2 phosphate (2-P-cADPR) from NADP.. Terms. ADP-ribosyl Cyclase Preferred ...
... bpoc adp-ribosyl cyclase/cyclic adp-ribose hydrolase,adp-ribosyl cyclase\/cyclic adp-ribose hydrolase,C1413221,cd38 molecule ... guanylate cyclase, soluble, beta-2,gngm female genital vein,female genital vein,C0729954,female genital vein,bpoc cacna1a, ... trf1-interacting ankyrin-related adp-ribose polymerase wt allele,gngm tinf2,tinf2,C1336631,tinf2,gngm pc,pc,C1418943,proc,gngm ...
Subcellular localization of cyclic ADP-ribosyl cyclase and cyclic ADP-ribose hydrolase activities in porcine airway smooth ... Role of cyclic ADP-ribose in the regulation of [Ca2+](i) in porcine tracheal smooth muscle. Prakash, Y. S., Kannan, M. S., ... The Role of Cyclic-ADP-Ribose-Signaling Pathway in Oxytocin-Induced Ca 2+ Transients in Human Myometrium Cells. Barata, H., ... Soluble guanylate cyclase modulators blunt hyperoxia effects on calcium responses of developing human airway smooth muscle. ...
  • The type II transmembrane glycoprotein CD38 (ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase) has been proposed as a mediator of insulin secretion from pancreatic β-cells and as a candidate for autoimmune reactions in type 2 diabetes. (elsevier.com)
  • By functioning as both a cyclase and a hydrolase, CD38 mediates lymphocyte activation, adhesion, and the metabolism of cADPR and NAADP. (biolegend.com)
  • 10 mU/mg, as measured under the described conditions using BioVision's CD38 (Cyclase) Activity Assay Kit (Cat. (transcriptionfactor.org)
  • CD38, also known as cyclic ADP ribose hydrolase is a glycoprotein found on the surface of many immune cells (white blood cells), including CD4+, CD8+, B lymphocytes and natural killer cells. (transcriptionfactor.org)
  • CD38 is a multifunctional ectoenzyme that catalyzes the synthesis and hydrolysis of cyclic ADP-ribose (cADPR) from NAD+ to ADP-ribose. (transcriptionfactor.org)
  • Biomolecule/Target: CD38Synonyms: CD38, T10, cADPr hydrolase 1Alternates names: CD38, T10, cADPr hydrolase 1Taglines: Synthesizes the second messagers cyclic ADP-ribose and nicotinate-adenine. (transcriptionfactor.org)
  • Both are metabolites of pyridine nucleotides, and may be produced by the same class of enzymes, ADP-ribosyl cyclases, such as CD38. (ox.ac.uk)
  • 20,Evolution and function of the ADP ribosyl cyclase/CD38 gene family in physiology and pathology. (nmninfo.com)
  • The signaling cascade triggered by ABA in insulin releasing cells sequentially involves a pertussis toxin-sensitive G protein, cAMP overproduction, protein kinase A-mediated activation of the ADP-ribosyl cyclase CD38, and cyclic ADP-ribose overproduction. (unige.it)
  • We found that HOBIT human osteoblastic cells, which are known to express ADP-ribosyl cyclase/CD38 activity, respond to micromolar concentrations of extracellular NAD(+) with oscillatory increases of the cytosolic Ca(2+) concentration. (heightquest.com)
  • remain to be clearly identified (20), although members of the ADP-ribosyl cyclase family are possibly involved because the purified CD38 and ADP-ribosyl cyclases have been shown to be capable of NAADP synthesis (21,C23). (casein-kinases.com)
  • In enzymology, a ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase (EC 3.2.2.6) is a bifunctional enzyme that catalyzes the chemical reaction NAD+ + H2O ⇌ {\displaystyle \rightleftharpoons } cADPR + H2O + nicotinamide ⇌ {\displaystyle \rightleftharpoons } ADP-ribose + nicotinamide The 3 substrates of this enzyme are NAD+ and H2O, whereas its two products are ADP-ribose and nicotinamide. (wikipedia.org)
  • It is an ADP-ribosyl hydrolase expressed at variable levels on hematopoietic cells and in some non-hematopoietic tissues (such as brain, muscles, and kidney). (biolegend.com)
  • Synthesizes the second messengers cyclic ADP-ribose and nicotinate-adenine dinucleotide phosphate, the former a second messenger for glucose-induced insulin secretion. (euromabnet.com)
  • From trials, NAADP and the related Ca2+-mobilizing messenger, cyclic ADP-ribose (cADPR) can end up being synthesized by Compact disc38 using alternative substrates and with different pH dependences (24). (casein-kinases.com)
  • Rabbit Polyclonal to OR2B6 Nevertheless, amazingly, just one research provides connected the creation of endogenous NAADP to any member of the ADP-ribosyl cyclase family members (25) and a physical function for Compact disc38 as an NAADP synthase provides been reigned over out by some (17, 18). (casein-kinases.com)
  • In mammals, mono-ADP-ribosylation serves to regulate protein functions, and it is catalysed by two families of toxin-related cellular ADP-ribosyltransferases: ecto-enzymes that modify various cell-surface proteins, like integrins and receptors, and intracellular enzymes that act on a variety of nuclear and cytosolic proteins. (imrpress.com)
  • 16,Crosstalk between poly(ADP-ribose) polymerase and sirtuin enzymes. (nmninfo.com)
  • 17,New insights into the molecular and cellular functions of poly(ADP-ribose) and PARPs. (nmninfo.com)
  • G244-LM is a potent and specific small-molecule tankyrase inhibitors, which reduces Wnt/β-catenin signaling by preventing poly(ADP-ribosyl)ation-dependent AXIN degradation, thereby promoting β-catenin destabilization. (biochempartner.com)
  • 2008 ) Regulation of nuclear Ca2+ signaling by translocation of the Ca2+ messenger synthesizing enzyme ADP-ribosyl cyclase during neuronal depolarization. (neurotree.org)
  • First enzymatic synthesis of an N1-cyclised cADPR (cyclic-ADP ribose) analogue with a hypoxanthine partial structure: discovery of a membrane permeant cADPR agonist. (ox.ac.uk)
  • Mono-ADP-ribosylation is a post-translational modification that was discovered more than five decades ago, and it consists of the enzymatic transfer of ADP-ribose from NAD + to acceptor proteins. (imrpress.com)
  • Edema factor, the enzymatic A subunit of EdTx, is an adenylate cyclase. (aai.org)
  • Recently, new examples of ADP-ribosylation of proteins involved in signal transduction and intracellular trafficking have been discovered, thus opening the route to the better molecular understanding of this reaction and of its role in human cell physiology and pathology. (imrpress.com)
  • In addition, two members of the structurally non-related sirtuin family can also modify cellular proteins by mono-ADP-ribosylation. (imrpress.com)
  • In viruses and prokaryotes, mono-ADP-ribosylation is mainly, but not exclusively, a mechanism used to take control of the host cell. (imrpress.com)
  • The reaction proceeds through cyclic ADP-ribose (cADPR) as intermediate, which is then hydrolyzed into ADP-ribose. (wikipedia.org)
  • Nicotinamide 8-Br-hypoxanthine dinucleotide (8-Br-NHD+) was cyclised at the N1 position by the ADP-ribosyl cyclase from Aplysia californica to give cyclic 8-Br-inosine diphosphoribose (8-Br-N1-cIDPR), a novel membrane-permeant agonist of Ca2+ release in human T cells. (ox.ac.uk)
  • Abscisic acid is an endogenous stimulator of insulin release from human pancreatic islets with cyclic ADP-ribose as second messenger. (unige.it)
  • Diadenosine homodinucleotide products of ADP-ribosyl cyclases behave as modulators of the purinergic receptor P2X7. (unige.it)
  • We examined lysates and microsomes of T. gondii and detected evidence of cADPR (cyclic ADP ribose) cyclase and hydrolase activities, the two enzymes that control the second messenger cADPR, which causes calcium release from RyR (ryanodine receptor). (elsevier.com)
  • Spatiotemporal Changes in NADH and Cyclic ADP Ribose During Ischemia One of the strengths of IMS lies in its ability to identify metabolites in a nontargeted manner. (cancer-ecosystem.com)
  • In this study, NADH and cyclic ADP-ribose (cADPR) are among such metabolites identified retrospectively by tandem MS/MS analyses (Table 1). (cancer-ecosystem.com)
  • Abstract: CD157 is a GPI-anchored ectoenzyme belonging to the NADase/ADP-ribosyl cyclase gene family involved in the control of human neutrophils and monocytes adhesion, migration and diapedesis. (unito.it)
  • It is an ADP-ribosyl hydrolase expressed at variable levels on hematopoietic cells and in some non-hematopoietic tissues (such as brain, muscles, and kidney). (biolegend.com)
  • Maps for ATP, ADP, and BYL719 inhibitor database AMP are constructed on the same tissue. (cancer-ecosystem.com)
  • A poly(ADP-ribose) polymerase (PARP) -1 and -2 inhibitor with chemosensitizing and antitumor activities. (mt911.com)
  • BST-1(bone marrow stromal cell antigen 1), also named as CD157 or ADP-ribosyl cyclase 2, is a glycosyl phosphotidylinositol-anchored protein that stimulates pre-B-cell growth and has adenosine diphosphate (ADP)-ribosyl cyclase and cyclic ADP-ribose (cADPR) hydrolase activity (PMID: 9473467, 11866528). (ptglab.com)
  • Since the Ca2+-mobilizing effects of NO can be mimicked by cGMP, are inhibited by the cGMP-dependent-protein kinase inhibitor, Rp-8-pCPT-cGMPS, and in egg homogenates show a requirement for the guanylyl cyclase substrate, GTP, we suggest a novel action of NO in mobilizing intracellular calcium from microsomal stores via a signaling pathway involving cGMP and cADPR. (ox.ac.uk)
  • However, to date, the upstream guanylyl cyclase (GC) subtype eliciting cGMP production is unknown. (frontiersin.org)
  • Crystallographic studies on human BST-1/CD157 with ADP-ribosyl cyclase and NAD glycohydrolase activities. (expasy.org)
  • Titolo: Functional Role and Prognostic Significance of CD157 in Ovarian Carcinoma Abstract: Background: CD157, an ADP-ribosyl cyclase-related cell surface molecule, regulates leukocyte diapedesis during inflammation. (unito.it)
  • A membrane-bound or cytosolic enzyme that catalyzes the synthesis of CYCLIC ADP-RIBOSE (cADPR) from nicotinamide adenine dinucleotide ( NAD ). (nih.gov)
  • This enzyme generally catalyzes the hydrolysis of cADPR to ADP-RIBOSE , as well, and sometimes the synthesis of cyclic ADP-ribose 2' phosphate (2'-P-cADPR) from NADP . (nih.gov)
  • A bifunctional enzyme that catalyzes the synthesis and HYDROLYSIS of CYCLIC ADP-RIBOSE (cADPR) from NAD+ to ADP-RIBOSE. (lookformedical.com)
  • mice that absence the main calcium-mobilizing second messenger cyclic ADP ribose. (bus2226w16.com)
  • Nitric oxide-induced mobilization of intracellular calcium via the cyclic ADP-ribose signaling pathway. (ox.ac.uk)
  • Cyclic adenosine diphosphate ribose (cADPR) is a potent endogenous calcium-mobilizing agent synthesized from beta-NAD+ by ADP-ribosyl cyclases in sea urchin eggs and in several mammalian cells (Galione, A., and White, A. (1994) Trends Cell Biol. (ox.ac.uk)
  • OHCs in these frequency regions in young GC-A knockout mice exhibited diminished levels of KCNQ4 expression, which is the dominant K + channel in OHCs, and decreased activation of poly (ADP-ribose) polymerase-1, an enzyme involved in DNA repair. (frontiersin.org)
  • NAD is a precursor to biologically active metabolites such as ADPR, ADP_ribosyl-derivatives and cADPR. (bl.uk)
  • While three different enzymes have been identified as cyclases, the mechanism and site of activity of such enzymes has not yet been fully established. (bl.uk)
  • Protein mono-ADP-ribosylation is a reversible post-translational modification that plays a role in the regulation of cellular activities [4]. (enzyme-database.org)
  • The enzyme from some, but not all, species can also use NADP + as acceptor (giving rise to N ω -[(2′-phospho-ADP)- D -ribosyl]-protein- L -arginine as the product), but more slowly [1,5]. (enzyme-database.org)
  • Corda, D. and Di Girolamo, M. Functional aspects of protein mono-ADP-ribosylation. (enzyme-database.org)
  • In the rat, we have previously reported strong ADP-ribosyl cyclase activity in the sublingual salivary gland (Masuda W. and Noguchi T. Biochem. (elsevier.com)
  • The enzyme catalyses the NAD + -dependent activation of EC 4.6.1.1 , adenylate cyclase. (enzyme-database.org)
  • The aim of this work is thus to synthesise base-modified NAD+ analogues that can reach the cyclase active site, and be enzymatically converted to cyclic products that fluoresce at wavelengths not harmful to cells. (bl.uk)
  • cADPR is a Ca 2 -modulating second messenger formed by the cyclisation of NAD+ by ADP-ribosyl cyclases. (bl.uk)