Form of passive immunization where previously sensitized immunologic agents (cells or serum) are transferred to non-immune recipients. When transfer of cells is used as a therapy for the treatment of neoplasms, it is called adoptive immunotherapy (IMMUNOTHERAPY, ADOPTIVE).
Form of adoptive transfer where cells with antitumor activity are transferred to the tumor-bearing host in order to mediate tumor regression. The lymphoid cells commonly used are lymphokine-activated killer (LAK) cells and tumor-infiltrating lymphocytes (TIL). This is usually considered a form of passive immunotherapy. (From DeVita, et al., Cancer, 1993, pp.305-7, 314)
Transfer of immunity from immunized to non-immune host by administration of serum antibodies, or transplantation of lymphocytes (ADOPTIVE TRANSFER).
The introduction of functional (usually cloned) GENES into cells. A variety of techniques and naturally occurring processes are used for the gene transfer such as cell hybridization, LIPOSOMES or microcell-mediated gene transfer, ELECTROPORATION, chromosome-mediated gene transfer, TRANSFECTION, and GENETIC TRANSDUCTION. Gene transfer may result in genetically transformed cells and individual organisms.
An encapsulated lymphatic organ through which venous blood filters.
A critical subpopulation of T-lymphocytes involved in the induction of most immunological functions. The HIV virus has selective tropism for the T4 cell which expresses the CD4 phenotypic marker, a receptor for HIV. In fact, the key element in the profound immunosuppression seen in HIV infection is the depletion of this subset of T-lymphocytes.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
A critical subpopulation of regulatory T-lymphocytes involved in MHC Class I-restricted interactions. They include both cytotoxic T-lymphocytes (T-LYMPHOCYTES, CYTOTOXIC) and CD8+ suppressor T-lymphocytes.
The transfer of mammalian embryos from an in vivo or in vitro environment to a suitable host to improve pregnancy or gestational outcome in human or animal. In human fertility treatment programs, preimplantation embryos ranging from the 4-cell stage to the blastocyst stage are transferred to the uterine cavity between 3-5 days after FERTILIZATION IN VITRO.
The naturally occurring transmission of genetic information between organisms, related or unrelated, circumventing parent-to-offspring transmission. Horizontal gene transfer may occur via a variety of naturally occurring processes such as GENETIC CONJUGATION; GENETIC TRANSDUCTION; and TRANSFECTION. It may result in a change of the recipient organism's genetic composition (TRANSFORMATION, GENETIC).
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.
CD4-positive T cells that inhibit immunopathology or autoimmune disease in vivo. They inhibit the immune response by influencing the activity of other cell types. Regulatory T-cells include naturally occurring CD4+CD25+ cells, IL-10 secreting Tr1 cells, and Th3 cells.
The transfer of lymphocytes from a donor to a recipient or reinfusion to the donor.
A classification of T-lymphocytes, especially into helper/inducer, suppressor/effector, and cytotoxic subsets, based on structurally or functionally different populations of cells.
The specific failure of a normally responsive individual to make an immune response to a known antigen. It results from previous contact with the antigen by an immunologically immature individual (fetus or neonate) or by an adult exposed to extreme high-dose or low-dose antigen, or by exposure to radiation, antimetabolites, antilymphocytic serum, etc.
An albumin obtained from the white of eggs. It is a member of the serpin superfamily.
Mice homozygous for the mutant autosomal recessive gene "scid" which is located on the centromeric end of chromosome 16. These mice lack mature, functional lymphocytes and are thus highly susceptible to lethal opportunistic infections if not chronically treated with antibiotics. The lack of B- and T-cell immunity resembles severe combined immunodeficiency (SCID) syndrome in human infants. SCID mice are useful as animal models since they are receptive to implantation of a human immune system producing SCID-human (SCID-hu) hematochimeric mice.
Specialized cells of the hematopoietic system that have branch-like extensions. They are found throughout the lymphatic system, and in non-lymphoid tissues such as SKIN and the epithelia of the intestinal, respiratory, and reproductive tracts. They trap and process ANTIGENS, and present them to T-CELLS, thereby stimulating CELL-MEDIATED IMMUNITY. They are different from the non-hematopoietic FOLLICULAR DENDRITIC CELLS, which have a similar morphology and immune system function, but with respect to humoral immunity (ANTIBODY PRODUCTION).
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES.
They are oval or bean shaped bodies (1 - 30 mm in diameter) located along the lymphatic system.
Change in learning in one situation due to prior learning in another situation. The transfer can be positive (with second learning improved by first) or negative (where the reverse holds).
Immunized T-lymphocytes which can directly destroy appropriate target cells. These cytotoxic lymphocytes may be generated in vitro in mixed lymphocyte cultures (MLC), in vivo during a graft-versus-host (GVH) reaction, or after immunization with an allograft, tumor cell or virally transformed or chemically modified target cell. The lytic phenomenon is sometimes referred to as cell-mediated lympholysis (CML). These CD8-positive cells are distinct from NATURAL KILLER CELLS and NATURAL KILLER T-CELLS. There are two effector phenotypes: TC1 and TC2.
The altered state of immunologic responsiveness resulting from initial contact with antigen, which enables the individual to produce antibodies more rapidly and in greater quantity in response to secondary antigenic stimulus.
An experimental animal model for central nervous system demyelinating disease. Inoculation with a white matter emulsion combined with FREUND'S ADJUVANT, myelin basic protein, or purified central myelin triggers a T cell-mediated immune response directed towards central myelin. The pathologic features are similar to MULTIPLE SCLEROSIS, including perivascular and periventricular foci of inflammation and demyelination. Subpial demyelination underlying meningeal infiltrations also occurs, which is also a feature of ENCEPHALOMYELITIS, ACUTE DISSEMINATED. Passive immunization with T-cells from an afflicted animal to a normal animal also induces this condition. (From Immunol Res 1998;17(1-2):217-27; Raine CS, Textbook of Neuropathology, 2nd ed, p604-5)
Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.
Subset of helper-inducer T-lymphocytes which synthesize and secrete interleukin-2, gamma-interferon, and interleukin-12. Due to their ability to kill antigen-presenting cells and their lymphokine-mediated effector activity, Th1 cells are associated with vigorous delayed-type hypersensitivity reactions.
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
Disorders that are characterized by the production of antibodies that react with host tissues or immune effector cells that are autoreactive to endogenous peptides.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
A type of FLUORESCENCE SPECTROSCOPY using two FLUORESCENT DYES with overlapping emission and absorption spectra, which is used to indicate proximity of labeled molecules. This technique is useful for studying interactions of molecules and PROTEIN FOLDING.
The phenomenon of target cell destruction by immunologically active effector cells. It may be brought about directly by sensitized T-lymphocytes or by lymphoid or myeloid "killer" cells, or it may be mediated by cytotoxic antibody, cytotoxic factor released by lymphoid cells, or complement.
Experimentally induced tumor that produces MELANIN in animals to provide a model for studying human MELANOMA.
Manifestations of the immune response which are mediated by antigen-sensitized T-lymphocytes via lymphokines or direct cytotoxicity. This takes place in the absence of circulating antibody or where antibody plays a subordinate role.
Antigenic determinants recognized and bound by the T-cell receptor. Epitopes recognized by the T-cell receptor are often located in the inner, unexposed side of the antigen, and become accessible to the T-cell receptors after proteolytic processing of the antigen.
A strain of non-obese diabetic mice developed in Japan that has been widely studied as a model for T-cell-dependent autoimmune insulin-dependent diabetes mellitus in which insulitis is a major histopathologic feature, and in which genetic susceptibility is strongly MHC-linked.
Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
Molecules on the surface of T-lymphocytes that recognize and combine with antigens. The receptors are non-covalently associated with a complex of several polypeptides collectively called CD3 antigens (ANTIGENS, CD3). Recognition of foreign antigen and the major histocompatibility complex is accomplished by a single heterodimeric antigen-receptor structure, composed of either alpha-beta (RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA) or gamma-delta (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA) chains.
An increased reactivity to specific antigens mediated not by antibodies but by cells.
The small RNA molecules, 73-80 nucleotides long, that function during translation (TRANSLATION, GENETIC) to align AMINO ACIDS at the RIBOSOMES in a sequence determined by the mRNA (RNA, MESSENGER). There are about 30 different transfer RNAs. Each recognizes a specific CODON set on the mRNA through its own ANTICODON and as aminoacyl tRNAs (RNA, TRANSFER, AMINO ACYL), each carries a specific amino acid to the ribosome to add to the elongating peptide chains.
Subset of helper-inducer T-lymphocytes which synthesize and secrete the interleukins IL-4, IL-5, IL-6, and IL-10. These cytokines influence B-cell development and antibody production as well as augmenting humoral responses.
Substances that are recognized by the immune system and induce an immune reaction.
Immunosuppression by reduction of circulating lymphocytes or by T-cell depletion of bone marrow. The former may be accomplished in vivo by thoracic duct drainage or administration of antilymphocyte serum. The latter is performed ex vivo on bone marrow before its transplantation.
Interfacility or intrahospital transfer of patients. Intrahospital transfer is usually to obtain a specific kind of care and interfacility transfer is usually for economic reasons as well as for the type of care provided.
A ubiquitous family of proteins that transport PHOSPHOLIPIDS such as PHOSPHATIDYLINOSITOL and PHOSPHATIDYLCHOLINE between membranes. They play an important role in phospholipid metabolism during vesicular transport and SIGNAL TRANSDUCTION.
A soluble substance elaborated by antigen- or mitogen-stimulated T-LYMPHOCYTES which induces DNA synthesis in naive lymphocytes.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.
Resistance to a disease-causing agent induced by the introduction of maternal immunity into the fetus by transplacental transfer or into the neonate through colostrum and milk.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).
The transfer of bacterial DNA by phages from an infected bacterium to another bacterium. This also refers to the transfer of genes into eukaryotic cells by viruses. This naturally occurring process is routinely employed as a GENE TRANSFER TECHNIQUE.
A transmembrane protein present in the MYELIN SHEATH of the CENTRAL NERVOUS SYSTEM. It is one of the main autoantigens implicated in the pathogenesis of MULTIPLE SCLEROSIS.
The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.
Factor derived from leukocyte lysates of immune donors which can transfer both local and systemic cellular immunity to nonimmune recipients.
All of the processes involved in increasing CELL NUMBER including CELL DIVISION.
Bone marrow-derived lymphocytes that possess cytotoxic properties, classically directed against transformed and virus-infected cells. Unlike T CELLS; and B CELLS; NK CELLS are not antigen specific. The cytotoxicity of natural killer cells is determined by the collective signaling of an array of inhibitory and stimulatory CELL SURFACE RECEPTORS. A subset of T-LYMPHOCYTES referred to as NATURAL KILLER T CELLS shares some of the properties of this cell type.
Lymphocytes that show specificity for autologous tumor cells. Ex vivo isolation and culturing of TIL with interleukin-2, followed by reinfusion into the patient, is one form of adoptive immunotherapy of cancer.
Deliberate prevention or diminution of the host's immune response. It may be nonspecific as in the administration of immunosuppressive agents (drugs or radiation) or by lymphocyte depletion or may be specific as in desensitization or the simultaneous administration of antigen and immunosuppressive drugs.
A low affinity interleukin-2 receptor subunit that combines with the INTERLEUKIN-2 RECEPTOR BETA SUBUNIT and the INTERLEUKIN RECEPTOR COMMON GAMMA-CHAIN to form a high affinity receptor for INTERLEUKIN-2.
A cytokine produced by a variety of cell types, including T-LYMPHOCYTES; MONOCYTES; DENDRITIC CELLS; and EPITHELIAL CELLS that exerts a variety of effects on immunoregulation and INFLAMMATION. Interleukin-10 combines with itself to form a homodimeric molecule that is the biologically active form of the protein.
A subclass of winged helix DNA-binding proteins that share homology with their founding member fork head protein, Drosophila.
The grafting of skin in humans or animals from one site to another to replace a lost portion of the body surface skin.
Process of classifying cells of the immune system based on structural and functional differences. The process is commonly used to analyze and sort T-lymphocytes into subsets based on CD antigens by the technique of flow cytometry.
A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)
Techniques and strategies which include the use of coding sequences and other conventional or radical means to transform or modify cells for the purpose of treating or reversing disease conditions.
Elements of limited time intervals, contributing to particular results or situations.
A parasexual process in BACTERIA; ALGAE; FUNGI; and ciliate EUKARYOTA for achieving exchange of chromosome material during fusion of two cells. In bacteria, this is a uni-directional transfer of genetic material; in protozoa it is a bi-directional exchange. In algae and fungi, it is a form of sexual reproduction, with the union of male and female gametes.
Manipulation of the host's immune system in treatment of disease. It includes both active and passive immunization as well as immunosuppressive therapy to prevent graft rejection.
Differentiation antigens found on thymocytes and on cytotoxic and suppressor T-lymphocytes. CD8 antigens are members of the immunoglobulin supergene family and are associative recognition elements in MHC (Major Histocompatibility Complex) Class I-restricted interactions.
A specific immune response elicited by a specific dose of an immunologically active substance or cell in an organism, tissue, or cell.
Process whereby the immune system reacts against the body's own tissues. Autoimmunity may produce or be caused by AUTOIMMUNE DISEASES.
A proinflammatory cytokine produced primarily by T-LYMPHOCYTES or their precursors. Several subtypes of interleukin-17 have been identified, each of which is a product of a unique gene.
Transplantation between individuals of the same species. Usually refers to genetically disparate individuals in contradistinction to isogeneic transplantation for genetically identical individuals.
Reduction in the number of lymphocytes.
Proteins, glycoprotein, or lipoprotein moieties on surfaces of tumor cells that are usually identified by monoclonal antibodies. Many of these are of either embryonic or viral origin.
Methods of implanting a CELL NUCLEUS from a donor cell into an enucleated acceptor cell.
Endogenous tissue constituents that have the ability to interact with AUTOANTIBODIES and cause an immune response.
The process by which ELECTRONS are transported from a reduced substrate to molecular OXYGEN. (From Bennington, Saunders Dictionary and Encyclopedia of Laboratory Medicine and Technology, 1984, p270)
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
Inflammation of the RETINA. It is rarely limited to the retina, but is commonly associated with diseases of the choroid (CHORIORETINITIS) and of the OPTIC DISK (neuroretinitis).
Subset of helper-effector T-lymphocytes which synthesize and secrete IL-17, IL-17F, and IL-22. These cytokines are involved in host defenses and tissue inflammation in autoimmune diseases.
Antigens that exist in alternative (allelic) forms in a single species. When an isoantigen is encountered by species members who lack it, an immune response is induced. Typical isoantigens are the BLOOD GROUP ANTIGENS.
Subpopulation of CD4+ lymphocytes that cooperate with other lymphocytes (either T or B) to initiate a variety of immune functions. For example, helper-inducer T-cells cooperate with B-cells to produce antibodies to thymus-dependent antigens and with other subpopulations of T-cells to initiate a variety of cell-mediated immune functions.
An integrin alpha subunit of approximately 150-kDa molecular weight. It is expressed at high levels on monocytes and combines with CD18 ANTIGEN to form the cell surface receptor INTEGRIN ALPHAXBETA2. The subunit contains a conserved I-domain which is characteristic of several of alpha integrins.
An organism whose body contains cell populations of different genotypes as a result of the TRANSPLANTATION of donor cells after sufficient ionizing radiation to destroy the mature recipient's cells which would otherwise reject the donor cells.
Surgical removal of the thymus gland. (Dorland, 28th ed)
Surgical procedure by which a tendon is incised at its insertion and placed at an anatomical site distant from the original insertion. The tendon remains attached at the point of origin and takes over the function of a muscle inactivated by trauma or disease.
A family of non-enveloped viruses infecting mammals (MASTADENOVIRUS) and birds (AVIADENOVIRUS) or both (ATADENOVIRUS). Infections may be asymptomatic or result in a variety of diseases.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.
The rate dynamics in chemical or physical systems.
The process by which antigen is presented to lymphocytes in a form they can recognize. This is performed by antigen presenting cells (APCs). Some antigens require processing before they can be recognized. Antigen processing consists of ingestion and partial digestion of the antigen by the APC, followed by presentation of fragments on the cell surface. (From Rosen et al., Dictionary of Immunology, 1989)
A soluble factor produced by activated T-LYMPHOCYTES that induces the expression of MHC CLASS II GENES and FC RECEPTORS on B-LYMPHOCYTES and causes their proliferation and differentiation. It also acts on T-lymphocytes, MAST CELLS, and several other hematopoietic lineage cells.
A CD antigen that contains a conserved I domain which is involved in ligand binding. When combined with CD18 the two subunits form MACROPHAGE-1 ANTIGEN.
A heterogeneous group of immunocompetent cells that mediate the cellular immune response by processing and presenting antigens to the T-cells. Traditional antigen-presenting cells include MACROPHAGES; DENDRITIC CELLS; LANGERHANS CELLS; and B-LYMPHOCYTES. FOLLICULAR DENDRITIC CELLS are not traditional antigen-presenting cells, but because they hold antigen on their cell surface in the form of IMMUNE COMPLEXES for B-cell recognition they are considered so by some authors.
A single, unpaired primary lymphoid organ situated in the MEDIASTINUM, extending superiorly into the neck to the lower edge of the THYROID GLAND and inferiorly to the fourth costal cartilage. It is necessary for normal development of immunologic function early in life. By puberty, it begins to involute and much of the tissue is replaced by fat.
Established cell cultures that have the potential to propagate indefinitely.
Genes involved in activating the enzyme VDJ recombinase. RAG-1 is located on chromosome 11 in humans (chromosome 2 in mice) and is expressed exclusively in maturing lymphocytes.
Glycoproteins found on the membrane or surface of cells.
55-kDa antigens found on HELPER-INDUCER T-LYMPHOCYTES and on a variety of other immune cell types. CD4 antigens are members of the immunoglobulin supergene family and are implicated as associative recognition elements in MAJOR HISTOCOMPATIBILITY COMPLEX class II-restricted immune responses. On T-lymphocytes they define the helper/inducer subset. CD4 antigens also serve as INTERLEUKIN-15 receptors and bind to the HIV receptors, binding directly to the HIV ENVELOPE PROTEIN GP120.
Cell adhesion molecule and CD antigen that serves as a homing receptor for lymphocytes to lymph node high endothelial venules.
T-cell receptors composed of CD3-associated alpha and beta polypeptide chains and expressed primarily in CD4+ or CD8+ T-cells. Unlike immunoglobulins, the alpha-beta T-cell receptors recognize antigens only when presented in association with major histocompatibility (MHC) molecules.
The property of the T-CELL RECEPTOR which enables it to react with some antigens and not others. The specificity is derived from the structure of the receptor's variable region which has the ability to recognize certain antigens in conjunction with the MAJOR HISTOCOMPATIBILITY COMPLEX molecule.
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
Experimental transplantation of neoplasms in laboratory animals for research purposes.
Mice bearing mutant genes which are phenotypically expressed in the animals.
Inflammation of the COLON section of the large intestine (INTESTINE, LARGE), usually with symptoms such as DIARRHEA (often with blood and mucus), ABDOMINAL PAIN, and FEVER.
Transplantation between genetically identical individuals, i.e., members of the same species with identical histocompatibility antigens, such as monozygotic twins, members of the same inbred strain, or members of a hybrid population produced by crossing certain inbred strains.
Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.
An abundant cytosolic protein that plays a critical role in the structure of multilamellar myelin. Myelin basic protein binds to the cytosolic sides of myelin cell membranes and causes a tight adhesion between opposing cell membranes.
A subtype of DIABETES MELLITUS that is characterized by INSULIN deficiency. It is manifested by the sudden onset of severe HYPERGLYCEMIA, rapid progression to DIABETIC KETOACIDOSIS, and DEATH unless treated with insulin. The disease may occur at any age, but is most common in childhood or adolescence.
An immune response with both cellular and humoral components, directed against an allogeneic transplant, whose tissue antigens are not compatible with those of the recipient.
Receptors present on activated T-LYMPHOCYTES and B-LYMPHOCYTES that are specific for INTERLEUKIN-2 and play an important role in LYMPHOCYTE ACTIVATION. They are heterotrimeric proteins consisting of the INTERLEUKIN-2 RECEPTOR ALPHA SUBUNIT, the INTERLEUKIN-2 RECEPTOR BETA SUBUNIT, and the INTERLEUKIN RECEPTOR COMMON GAMMA-CHAIN.
A technique of culturing mixed cell types in vitro to allow their synergistic or antagonistic interactions, such as on CELL DIFFERENTIATION or APOPTOSIS. Coculture can be of different types of cells, tissues, or organs from normal or disease states.
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
The number of LYMPHOCYTES per unit volume of BLOOD.
An induced state of non-reactivity to grafted tissue from a donor organism that would ordinarily trigger a cell-mediated or humoral immune response.
Inflammation of part or all of the uvea, the middle (vascular) tunic of the eye, and commonly involving the other tunics (sclera and cornea, and the retina). (Dorland, 27th ed)
A group of differentiation surface antigens, among the first to be discovered on thymocytes and T-lymphocytes. Originally identified in the mouse, they are also found in other species including humans, and are expressed on brain neurons and other cells.
The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.
A calcium-dependent pore-forming protein synthesized in cytolytic LYMPHOCYTES and sequestered in secretory granules. Upon immunological reaction between a cytolytic lymphocyte and a target cell, perforin is released at the plasma membrane and polymerizes into transmembrane tubules (forming pores) which lead to death of a target cell.
Vaccines or candidate vaccines designed to prevent or treat cancer. Vaccines are produced using the patient's own whole tumor cells as the source of antigens, or using tumor-specific antigens, often recombinantly produced.
Allelic variants of the immunoglobulin light chains (IMMUNOGLOBULIN LIGHT CHAINS) or heavy chains (IMMUNOGLOBULIN HEAVY CHAINS) encoded by ALLELES of IMMUNOGLOBULIN GENES.
Family of RNA viruses that infects birds and mammals and encodes the enzyme reverse transcriptase. The family contains seven genera: DELTARETROVIRUS; LENTIVIRUS; RETROVIRUSES TYPE B, MAMMALIAN; ALPHARETROVIRUS; GAMMARETROVIRUS; RETROVIRUSES TYPE D; and SPUMAVIRUS. A key feature of retrovirus biology is the synthesis of a DNA copy of the genome which is integrated into cellular DNA. After integration it is sometimes not expressed but maintained in a latent state (PROVIRUSES).
Forceful administration into the peritoneal cavity of liquid medication, nutrient, or other fluid through a hollow needle piercing the abdominal wall.
A form of meningitis caused by LYMPHOCYTIC CHORIOMENINGITIS VIRUS. MICE and other rodents serve as the natural hosts, and infection in humans usually occurs through inhalation or ingestion of infectious particles. Clinical manifestations include an influenza-like syndrome followed by stiff neck, alterations of mentation, ATAXIA, and incontinence. Maternal infections may result in fetal malformations and injury, including neonatal HYDROCEPHALUS, aqueductal stenosis, CHORIORETINITIS, and MICROCEPHALY. (From Joynt, Clinical Neurology, 1996, Ch26, pp1-3)
Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.
The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.
Infections with bacteria of the genus LISTERIA.
A pathological process characterized by injury or destruction of tissues caused by a variety of cytologic and chemical reactions. It is usually manifested by typical signs of pain, heat, redness, swelling, and loss of function.
A type of acute or chronic skin reaction in which sensitivity is manifested by reactivity to materials or substances coming in contact with the skin. It may involve allergic or non-allergic mechanisms.
Antibodies produced by a single clone of cells.
A group of lymphocyte surface antigens located on mouse LYMPHOCYTES. Specific Ly antigens are useful markers for distinguishing subpopulations of lymphocytes.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
The type species of ARENAVIRUS, part of the Old World Arenaviruses (ARENAVIRUSES, OLD WORLD), producing a silent infection in house and laboratory mice. In humans, infection with LCMV can be inapparent, or can present with an influenza-like illness, a benign aseptic meningitis, or a severe meningoencephalomyelitis. The virus can also infect monkeys, dogs, field mice, guinea pigs, and hamsters, the latter an epidemiologically important host.
The capacity of a normal organism to remain unaffected by microorganisms and their toxins. It results from the presence of naturally occurring ANTI-INFECTIVE AGENTS, constitutional factors such as BODY TEMPERATURE and immediate acting immune cells such as NATURAL KILLER CELLS.
A melanosome-associated protein that plays a role in the maturation of the MELANOSOME.
Nonsusceptibility to the invasive or pathogenic effects of foreign microorganisms or to the toxic effect of antigenic substances.
A classification of B-lymphocytes based on structurally or functionally different populations of cells.
The demonstration of the cytotoxic effect on a target cell of a lymphocyte, a mediator released by a sensitized lymphocyte, an antibody, or complement.
Differentiation antigens expressed on B-lymphocytes and B-cell precursors. They are involved in regulation of B-cell proliferation.
A form of hypersensitivity affecting the respiratory tract. It includes ASTHMA and RHINITIS, ALLERGIC, SEASONAL.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
A specialized subset of T-LYMPHOCYTES that exhibit features of INNATE IMMUNITY similar to that of NATURAL KILLER CELLS. They are reactive to glycolipids presented in the context of the major histocompatibility complex (MHC) class I-like molecule, CD1D ANTIGEN.
Specialized tissues that are components of the lymphatic system. They provide fixed locations within the body where a variety of LYMPHOCYTES can form, mature and multiply. The lymphoid tissues are connected by a network of LYMPHATIC VESSELS.
Mouse strains constructed to possess identical genotypes except for a difference at a single gene locus.
Large, transmembrane, non-covalently linked glycoproteins (alpha and beta). Both chains can be polymorphic although there is more structural variation in the beta chains. The class II antigens in humans are called HLA-D ANTIGENS and are coded by a gene on chromosome 6. In mice, two genes named IA and IE on chromosome 17 code for the H-2 antigens. The antigens are found on B-lymphocytes, macrophages, epidermal cells, and sperm and are thought to mediate the competence of and cellular cooperation in the immune response. The term IA antigens used to refer only to the proteins encoded by the IA genes in the mouse, but is now used as a generic term for any class II histocompatibility antigen.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Experimentally induced new abnormal growth of TISSUES in animals to provide models for studying human neoplasms.
Experimentally induced neoplasms of CONNECTIVE TISSUE in animals to provide a model for studying human SARCOMA.
Irradiation of the whole body with ionizing or non-ionizing radiation. It is applicable to humans or animals but not to microorganisms.
A myelin protein found in the periaxonal membrane of both the central and peripheral nervous systems myelin sheaths. It binds to cells surface receptors found on AXONS and may regulate cellular interactions between MYELIN and AXONS.
A melanosome-specific protein that plays a role in the expression, stability, trafficking, and processing of GP100 MELANOMA ANTIGEN, which is critical to the formation of Stage II MELANOSOMES. The protein is used as an antigen marker for MELANOMA cells.
Antigens expressed on the cell membrane of T-lymphocytes during differentiation, activation, and normal and neoplastic transformation. Their phenotypic characterization is important in differential diagnosis and studies of thymic ontogeny and T-cell function.
Transport proteins that carry specific substances in the blood or across cell membranes.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
A heterodimeric cytokine that plays a role in innate and adaptive immune responses. Interleukin-12 is a 70 kDa protein that is composed of covalently linked 40 kDa and 35 kDa subunits. It is produced by DENDRITIC CELLS; MACROPHAGES and a variety of other immune cells and plays a role in the stimulation of INTERFERON-GAMMA production by T-LYMPHOCYTES and NATURAL KILLER CELLS.
Proteins secreted from an organism which form membrane-spanning pores in target cells to destroy them. This is in contrast to PORINS and MEMBRANE TRANSPORT PROTEINS that function within the synthesizing organism and COMPLEMENT immune proteins. These pore forming cytotoxic proteins are a form of primitive cellular defense which are also found in human LYMPHOCYTES.
The transference of BONE MARROW from one human or animal to another for a variety of purposes including HEMATOPOIETIC STEM CELL TRANSPLANTATION or MESENCHYMAL STEM CELL TRANSPLANTATION.
The major group of transplantation antigens in the mouse.
Measure of histocompatibility at the HL-A locus. Peripheral blood lymphocytes from two individuals are mixed together in tissue culture for several days. Lymphocytes from incompatible individuals will stimulate each other to proliferate significantly (measured by tritiated thymidine uptake) whereas those from compatible individuals will not. In the one-way MLC test, the lymphocytes from one of the individuals are inactivated (usually by treatment with MITOMYCIN or radiation) thereby allowing only the untreated remaining population of cells to proliferate in response to foreign histocompatibility antigens.
Resistance to a disease agent resulting from the production of specific antibodies by the host, either after exposure to the disease or after vaccination.
Genes that are introduced into an organism using GENE TRANSFER TECHNIQUES.
Small antigenic determinants capable of eliciting an immune response only when coupled to a carrier. Haptens bind to antibodies but by themselves cannot elicit an antibody response.
Subunits of the antigenic determinant that are most easily recognized by the immune system and thus most influence the specificity of the induced antibody.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
The transference of a heart from one human or animal to another.
An individual that contains cell populations derived from different zygotes.
Sites on an antigen that interact with specific antibodies.
A cell line derived from cultured tumor cells.
Substances that augment, stimulate, activate, potentiate, or modulate the immune response at either the cellular or humoral level. The classical agents (Freund's adjuvant, BCG, Corynebacterium parvum, et al.) contain bacterial antigens. Some are endogenous (e.g., histamine, interferon, transfer factor, tuftsin, interleukin-1). Their mode of action is either non-specific, resulting in increased immune responsiveness to a wide variety of antigens, or antigen-specific, i.e., affecting a restricted type of immune response to a narrow group of antigens. The therapeutic efficacy of many biological response modifiers is related to their antigen-specific immunoadjuvanticity.
Cytokine that stimulates the proliferation of T-LYMPHOCYTES and shares biological activities with IL-2. IL-15 also can induce proliferation and differentiation of B-LYMPHOCYTES.
A general term for the complex phenomena involved in allo- and xenograft rejection by a host and graft vs host reaction. Although the reactions involved in transplantation immunology are primarily thymus-dependent phenomena of cellular immunity, humoral factors also play a part in late rejection.
A malignant neoplasm derived from cells that are capable of forming melanin, which may occur in the skin of any part of the body, in the eye, or, rarely, in the mucous membranes of the genitalia, anus, oral cavity, or other sites. It occurs mostly in adults and may originate de novo or from a pigmented nevus or malignant lentigo. Melanomas frequently metastasize widely, and the regional lymph nodes, liver, lungs, and brain are likely to be involved. The incidence of malignant skin melanomas is rising rapidly in all parts of the world. (Stedman, 25th ed; from Rook et al., Textbook of Dermatology, 4th ed, p2445)
The survival of a graft in a host, the factors responsible for the survival and the changes occurring within the graft during growth in the host.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
ARTHRITIS that is induced in experimental animals. Immunological methods and infectious agents can be used to develop experimental arthritis models. These methods include injections of stimulators of the immune response, such as an adjuvant (ADJUVANTS, IMMUNOLOGIC) or COLLAGEN.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
Costimulatory T-LYMPHOCYTE receptors that have specificity for CD80 ANTIGEN and CD86 ANTIGEN. Activation of this receptor results in increased T-cell proliferation, cytokine production and promotion of T-cell survival.
The experimental joining of two individuals for the purpose of studying the effects of one on the other.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
Surgical reinnervation of a denervated peripheral target using a healthy donor nerve and/or its proximal stump. The direct connection is usually made to a healthy postlesional distal portion of a non-functioning nerve or implanted directly into denervated muscle or insensitive skin. Nerve sprouts will grow from the transferred nerve into the denervated elements and establish contact between them and the neurons that formerly controlled another area.
Syndromes in which there is a deficiency or defect in the mechanisms of immunity, either cellular or humoral.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
The largest lymphatic vessel that passes through the chest and drains into the SUBCLAVIAN VEIN.
MYELIN-specific proteins that play a structural or regulatory role in the genesis and maintenance of the lamellar MYELIN SHEATH structure.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
A costimulatory ligand expressed by ANTIGEN-PRESENTING CELLS that binds to CTLA-4 ANTIGEN with high specificity and to CD28 ANTIGEN with low specificity. The interaction of CD80 with CD28 ANTIGEN provides a costimulatory signal to T-LYMPHOCYTES, while its interaction with CTLA-4 ANTIGEN may play a role in inducing PERIPHERAL TOLERANCE.

Prevention of collagen-induced arthritis by gene delivery of soluble p75 tumour necrosis factor receptor. (1/4012)

Collagen type II-induced arthritis (CIA) in DBA/1 mice can be passively transferred to SCID mice with spleen B- and T-lymphocytes. In the present study, we show that infection ex vivo of splenocytes from arthritic DBA/1 mice with a retroviral vector, containing cDNA for the soluble form of human p75 receptor of tumour necrosis factor (TNF-R) before transfer, prevents the development of arthritis, bone erosion and joint inflammation in the SCID recipients. Assessment of IgG subclass levels and studies of synovial histology suggest that down-regulating the effector functions of T helper-type 1 (Th1) cells may, at least in part, explain the inhibition of arthritis in the SCID recipients. In contrast, the transfer of splenocytes infected with mouse TNF-alpha gene construct resulted in exacerbated arthritis and enhancement of IgG2a antibody levels. Intriguingly, infection of splenocytes from arthritic DBA/1 mice with a construct for mouse IL-10 had no modulating effect on the transfer of arthritis. The data suggest that manipulation of the immune system with cytokines, or cytokine inhibitors using gene transfer protocols can be an effective approach to ameliorate arthritis.  (+info)

Thymic selection by a single MHC/peptide ligand: autoreactive T cells are low-affinity cells. (2/4012)

In H2-M- mice, the presence of a single peptide, CLIP, bound to MHC class II molecules generates a diverse repertoire of CD4+ cells. In these mice, typical self-peptides are not bound to class II molecules, with the result that a very high proportion of H2-M- CD4+ cells are responsive to the various peptides displayed on normal MHC-compatible APC. We show here, however, that such "self" reactivity is controlled by low-affinity CD4+ cells. These cells give spectacularly high proliferative responses but are virtually unreactive in certain other assays, e.g., skin graft rejection; responses to MHC alloantigens, by contrast, are intense in all assays. Possible explanations for why thymic selection directed to a single peptide curtails self specificity without affecting alloreactivity are discussed.  (+info)

Rapid death of adoptively transferred T cells in acquired immunodeficiency syndrome. (3/4012)

Human immunodeficiency virus (HIV)-specific cytotoxic T lymphocytes (CTL) probably play the major role in controlling HIV replication. However, the value of adoptive transfer of HIV-specific CTL expanded in vitro to HIV+ patients has been limited: this contrasts with the success of CTL therapy in treating or preventing Epstein-Barr virus and cytomegalovirus disease after bone marrow transplantation (BMT). We investigated the fate of expanded HIV-specific CTL clones in vivo following adoptive transfer to a patient with acquired immunodeficiency syndrome (AIDS). Two autologous CTL clones specific for HIV Gag and Pol were expanded to large numbers (>10(9)) in vitro and infused into an HIV-infected patient whose viral load was rising despite antiretroviral therapy. The fate of one clone was monitored by staining peripheral blood mononuclear cells (PBMCs) with T-cell receptor-specific tetrameric major histocompatibility complex (MHC)-peptide complexes. Although the CTL transfer was well tolerated, there were no significant changes in CD4 and CD8 lymphocyte counts and virus load. By tracking an infused clone using soluble MHC-peptide complexes, we show that cells bearing the Gag-specific T-cell receptors were rapidly eliminated within hours of infusion through apoptosis. Thus, the failure of adoptively transferred HIV-specific CTL to reduce virus load in AIDS may be due to rapid apoptosis of the infused cells, triggered by a number of potential mechanisms. Further trials of adoptive transfer of CTL should take into account the susceptibility of infused cells to in vivo apoptosis.  (+info)

Chlamydia infections and heart disease linked through antigenic mimicry. (4/4012)

Chlamydia infections are epidemiologically linked to human heart disease. A peptide from the murine heart muscle-specific alpha myosin heavy chain that has sequence homology to the 60-kilodalton cysteine-rich outer membrane proteins of Chlamydia pneumoniae, C. psittaci, and C. trachomatis was shown to induce autoimmune inflammatory heart disease in mice. Injection of the homologous Chlamydia peptides into mice also induced perivascular inflammation, fibrotic changes, and blood vessel occlusion in the heart, as well as triggering T and B cell reactivity to the homologous endogenous heart muscle-specific peptide. Chlamydia DNA functioned as an adjuvant in the triggering of peptide-induced inflammatory heart disease. Infection with C. trachomatis led to the production of autoantibodies to heart muscle-specific epitopes. Thus, Chlamydia-mediated heart disease is induced by antigenic mimicry of a heart muscle-specific protein.  (+info)

Adoptive transfer of genetically modified macrophages elucidated TGF-beta-mediated 'self-defence' of the glomerulus against local action of macrophages. (5/4012)

TGF-beta has several anti-inflammatory properties which may be relevant to prevention of or recovery from acute glomerular inflammation. Using genetically modified mesangial cells and a technique for in vivo macrophage transfer, this article provides evidence for TGF-beta-mediated 'self-defence' of the glomerulus against macrophages. Rat mesangial cells stably transfected with TGF-beta1 showed a blunted response to the macrophage-derived, proinflammatory cytokine IL-1beta. In contrast, mesangial cells expressing the dominant-interfering TGF-beta receptor showed an enhanced response to IL-1. Similarly, externally added TGF-beta1 inhibited the cytokine response of normal glomeruli, and isolated nephritic glomeruli producing active TGF-beta1 showed a depressed response to IL-1beta, compared to normal glomeruli. Consistent with these in vitro results, in vivo transfer of activated macrophages revealed that the TGF-beta-producing glomeruli are insensitive to the effector action of macrophages. These results indicate that TGF-beta1 functions as an endogenous 'defender' that counteracts local action of activated macrophages in the glomerulus.  (+info)

Efficient IgG-mediated suppression of primary antibody responses in Fcgamma receptor-deficient mice. (6/4012)

IgG antibodies can suppress more than 99% of the antibody response against the antigen to which they bind. This is used clinically to prevent rhesus-negative (Rh-) women from becoming immunized against Rh+ erythrocytes from their fetuses. The suppressive mechanism is poorly understood, but it has been proposed that IgG/erythrocyte complexes bind to the inhibitory Fc receptor for IgG (FcgammaRIIB) on the B cell surface, thereby triggering negative signals that turn off the B cell. We show that IgG induces the same degree of suppression of the response to sheep erythrocytes in animals lacking the known IgG-binding receptors FcgammaRIIB, FcgammaRI + III, FcgammaRI + IIB + III, and FcRn (the neonatal Fc receptor) as in wild-type animals. Reinvestigation of the ability of F(ab')2 fragments to suppress antibody responses demonstrated that they were nearly as efficient as intact IgG. In addition, monoclonal IgE also was shown to be suppressive. These findings suggest that IgG inhibits antibody responses through Fc-independent mechanisms, most likely by masking of antigenic epitopes, thereby preventing B cells from binding and responding to antigen. In agreement with this, we show that T cell priming is not abolished by passively administered IgG. The results have implications for the understanding of in vivo regulation of antibody responses and Rh prophylaxis.  (+info)

Tolerance to antigen-presenting cell-depleted islet allografts is CD4 T cell dependent. (7/4012)

Pretreatment of pancreatic islets in 95% oxygen culture depletes graft-associated APCs and leads to indefinite allograft acceptance in immunocompetent recipients. As such, the APC-depleted allograft represents a model of peripheral alloantigen presentation in the absence of donor-derived costimulation. Over time, a state of donor-specific tolerance develops in which recipients are resistant to donor APC-induced graft rejection. Thus, persistence of the graft is sufficient to induce tolerance independent of other immune interventions. Donor-specific tolerance could be adoptively transferred to immune-deficient SCID recipient mice transplanted with fresh immunogenic islet allografts, indicating that the original recipient was not simply "ignorant" of donor antigens. Interestingly, despite the fact that the original islet allograft presented only MHC class I alloantigens, CD8+ T cells obtained from tolerant animals readily collaborated with naive CD4+ T cells to reject donor-type islet grafts. Conversely, tolerant CD4+ T cells failed to collaborate effectively with naive CD8+ T cells for the rejection of donor-type grafts. In conclusion, the MHC class I+, II- islet allograft paradoxically leads to a change in the donor-reactive CD4 T cell subset and not in the CD8 subset. We hypothesize that the tolerant state is not due to direct class I alloantigen presentation to CD8 T cells but, rather, occurs via the indirect pathway of donor Ag presentation to CD4 T cells in the context of host MHC class II molecules.  (+info)

Mucosal immunity to influenza without IgA: an IgA knockout mouse model. (8/4012)

IgA knockout mice (IgA-/-) were generated by gene targeting and were used to determine the role of IgA in protection against mucosal infection by influenza and the value of immunization for preferential induction of secretory IgA. Aerosol challenge of naive IgA-/- mice and their wild-type IgA+/+ littermates with sublethal and lethal doses of influenza virus resulted in similar levels of pulmonary virus infection and mortality. Intranasal and i.p. immunization with influenza vaccine plus cholera toxin/cholera toxin B induced significant mucosal and serum influenza hemagglutinin-specific IgA Abs in IgA+/+ (but not IgA-/-) mice as well as IgG and IgM Abs in both IgA-/- and IgA+/+ mice; both exhibited similar levels of pulmonary and nasal virus replication and mortality following a lethal influenza virus challenge. Monoclonal anti-hemagglutinin IgG1, IgG2a, IgM, and polymeric IgA Abs were equally effective in preventing influenza virus infection in IgA-/- mice. These results indicate that IgA is not required for prevention of influenza virus infection and disease. Indeed, while mucosal immunization for selective induction of IgA against influenza may constitute a useful approach for control of influenza and other respiratory viral infections, strategies that stimulate other Igs in addition may be more desirable.  (+info)

TY - JOUR. T1 - Role of T cell receptor affinity in the efficacy and specificity of adoptive T cell therapies. AU - Stone, Jennifer D.. AU - Kranz, David M.. PY - 2013. Y1 - 2013. N2 - Over the last several years, there has been considerable progress in the treatment of cancer using gene modified adoptive T cell therapies. Two approaches have been used, one involving the introduction of a conventional αβ T cell receptor (TCR) against a pepMHC cancer antigen, and the second involving introduction of a chimeric antigen receptor (CAR) consisting of a single-chain antibody as an Fvfragment linked to transmembrane and signaling domains. In this review, we focus on one aspect of TCR-mediated adoptive T cell therapies, the impact of the affinity of the aß TCR for the pepMHC cancer antigen on both efficacy and specificity. We discuss the advantages of higher-affinity TCRs in mediating potent activity of CD4 T cells. This is balanced with the potential disadvantage of higher-affinity TCRs in mediating ...
Chronic lymphocytic leukemia (CLL) is an incurable adult disease of unknown etiology. Understanding the biology of CLL cells, particularly cell maturation and growth in vivo, has been impeded by lack of a reproducible adoptive transfer model. We report a simple, reproducible system in which primary CLL cells proliferate in nonobese diabetes/severe combined immunodeficiency/γc(null) mice under the influence of activated CLL-derived T lymphocytes. By co-transferring autologous T lymphocytes, activated in vivo by alloantigens, the survival and growth of primary CFSE-labeled CLL cells in vivo is achieved and quantified. Using this approach, we have identified key roles for CD4(+) T cells in CLL expansion, a direct link between CD38 expression by leukemic B cells and their activation, and support for CLL cells preferentially proliferating in secondary lymphoid tissues. The model should simplify analyzing kinetics of CLL cells in vivo, deciphering involvement of nonleukemic elements and nongenetic ...
PubMedID: 26100671 | Tumor-Specific Effector CD8+ T Cells That Can Establish Immunological Memory in Humans after Adoptive Transfer Are Marked by Expression of IL7 Receptor and c-myc. | Cancer Research | 8/15/2015
Adoptive transfer assay for TRAIL and CD69 expression on liver natural killer cells in response to starvation.(A) Isolated TRAIL− natural killer (NK) cells we
Immunotherapy is emerging as a major treatment for patients with cancer, predominantly via blocking immune inhibitory pathways and through adoptive T cell therapy. However, only a subset of patients shows clinical responses to these interventions. Emerging data indicates a correlation between clinic …
यहां हम वीवो बी-1ए सेल माइग्रेशन और स्थानीयकरण में जांच करने के लिए रेट्रोवायरल अतिअभिव्यक्ति और मूत्र बी-1ए कोशिकाओं के दत्तक...
There are many ways to fight cancer using the bodys own immune system. Some methods include the administration of vaccines while others involve stimulatory factors injected near tumors. One promising method is enlisting the help of T cells. To fight cancer effectively, T cells must be able to recognize cancerous antigens and the environment in which these T cells reside must be conducive to their function, survival, and proliferation. This paper discusses a method of providing such an environment called adoptive cell transfer, as well as the elements that effect this protocol and the ways in which the environment can be manipulated to increase the effectiveness of adoptive cell transfer. Many factors contribute to the observation that the effectiveness of adoptive cell transfer increases as immunodepletion increases, namely, the depletion of regulatory T cells. Additionally, the existence of natural killer cells during adoptive cell transfer has been shown to decrease its effectiveness. Also, increased
Adoptive T cell therapy to treat cancer in combination with re-directing specificity through T cell receptor (TCR) gene transfer, represents an effective therapeutic option. However, reduced effector responses due to the immunosuppressive tumour microenvironment and insufficient long-term engraftment of transferred cells represent two potential limitations. Tumours often employ mechanisms to inhibit T cell responses including secretion of TGFβ and depleting the tumour microenvironment of amino acids. The main aim of this PhD project was to develop a strategy to enhance T cell function for tumour therapy. The mammalian target of rapamycin (mTOR) pathway regulates CD8 T cell differentiation such that high mTOR activation leads to enhanced effector whilst low mTOR activation leads to increased T cell memory formation. Two retrovirus constructs have been designed whereby one expresses the positive mTOR regulator Rheb and the other expresses the negative mTOR regulator Pras40. Rheb transduction into ...
Complete cancer regression occurs in a subset of patients following adoptive T cell therapy (ACT) of ex vivo expanded tumor-infiltrating lymphocytes (TILs). However, the low success rate presents a great challenge to broader clinical application. To provide insight into TIL-based immunotherapy, we studied a successful case of ACT where regression was observed against tumors carrying the hotspot mutation G12D in the KRAS oncogene. Four T cell receptors (TCRs) made up the TIL infusion and recognized two KRAS-G12D neoantigens, a nonamer and a decamer, all restricted by human leukocyte antigen (HLA) C*08:02. Three of them (TCR9a, 9b, and 9c) were nonamer-specific, while one was decamer-specific (TCR10). We show that only mutant G12D but not the wild-type peptides stabilized HLA-C*08:02 due to the formation of a critical anchor salt bridge to HLA-C. Therapeutic TCRs exhibited high affinities, ranging from nanomolar to low micromolar. Intriguingly, TCR binding affinities to HLA-C inversely correlated ...
Interleukin-7 (IL-7) is an essential cytokine required for the development and maintenance of mature T cell. Its availability is limited under normal conditions, but rises during lymphopenia, leading to increased T cell proliferation. The administration of recombinant IL-7 to normal or lymphopenic mice and humans results in increased T cell numbers and altered T cell phenotype. Hence, IL-7 administration could mediate therapeutic benefits in immunocompromised patients and is currently tested in several clinical trials. However, besides its well-studied effects on T cells little is known about the effect of IL-7 on other immune and non-immune cells and their influence on T cell homeostasis. Therefore, we evaluated the effect of IL-7 therapy on adoptively transferred T cells in IL-7 receptor (IL-7R)-competent and IL-7R-deficient lymphopenic mice. We confirm the benefits of IL-7 therapy on T cell responses but additionally show that many of these effects are dependent on IL-7R expression by host ...
Mechanistically, the authors showed again that peripheral CD4+CD25+Foxp3+ Tregs mediated tolerance, while the results of experiments in T cell receptor transgenic animals showed no evidence for clonal deletion of MBP-reactive effector T cells, despite some level of expression in the thymus of liver MBP-transgenic mice (20). Importantly, expression of MBP in the skin did not protect against EAE, which indicates that expression of this protein in the hepatic environment is critical. In an elegant set of experiments based on adoptive transfer of Tregs and effector T cells labeled with fluorescent dye, the authors showed that Tregs induced by hepatic expression can turn effector T cells into Tregs by inducing expression of the transcription factor Foxp3. Effector T cells lacking TGF-β receptor II were resistant to this infectious tolerance mechanism, indicating dependence on TGF-β signaling. These findings are reminiscent of TGF-β-dependent suppression of CD8+ T cells by CD4+CD25+Foxp3+ Tregs ...
Purpose: : Th17 cells were identified to be highly immunopathogenic in several autoimmune models, including experimental autoimmune uveitis (EAU). More recently, however, the Th17 population was reported by Cuas group to also include a subset of non-pathogenic cells (McGeachy et al., Nature Immunol, 2007). Here we examined this dichotomy by a system of adoptively transferred ocular inflammation. Methods: : Naïve CD4 TCR transgenic cells specific against hen egg lysozyme (HEL) were activated with APC and HEL (APC/HEL), or with plate-bound anti-CD3/CD28 antibodies (PbAb) during polarization with IL-6 and TGF-beta. The cultured cells were tested for production of cytokines, chemokines and chemokine receptors, as well as their capacity to adoptively transfer ocular inflammation in recipient mice expressing HEL in their eyes. Results: : The two culturing procedures yielded populations with similar levels of IL-17 producing cells (~35%) and similar levels of IL-10, but with remarkably different ...
The reported duality of the MALT1 protease to promote cell intrinsic T‐cell effector functions and to suppress activated T cells in a Treg‐dependent manner raises a number of interesting questions. Even though the adoptive transfer demonstrates the potential of Tregs to counteract the autoimmunity, it remains open, if the reduced number of Tregs is indeed sufficient to cause the inflammatory phenotype. In the adoptive transfer experiment, only very few Tregs are sufficient to rescue the autoimmune phenotype raising the possibility that MALT1C472A/C472A Tregs may be less functional. In addition, the increase in IFN‐γ and IL‐4 production in MALT1 protease mutant mice may also indicate that deregulated T‐cell effector functions could also be involved in the early onset of gastritis, which is no longer counteracted due to the loss of peripheral T‐cell tolerance. In this respect, it remains an unresolved question why the autoimmune phenotype is manifested as gastritis. One can speculate ...
Purpose: To determine the antitumor efficacy and toxicity of a novel combination approach involving adoptive T cell immunotherapy utilizing chimeric antigen receptor (CAR) T cells with an immunomodulatory reagent for blocking immunosuppression. Experimental Design: We examined whether administration of a PD-1 blocking antibody could increase the therapeutic activity of CAR T cells against two different Her-2+ tumors. The use of a self-antigen mouse model enabled investigation into the efficacy, mechanism and toxicity of this combination approach. Results: In this study we first demonstrated a significant increase in the level of PD-1 expressed on transduced anti-Her-2 CD8+ T cells following antigen-specific stimulation with PD-L1+ tumor cells and that markers of activation and proliferation were increased in anti-Her-2 T cells in the presence of anti-PD-1 antibody. In adoptive transfer studies in Her-2 transgenic recipient mice, we demonstrated a significant improvement in growth inhibition of ...
Jin, L.-P., Zhou, Y.-H., Zhu, X.-Y., Wang, M.-Y. and Li, D.-J. (2006), Adoptive Transfer of Paternal Antigen-Hyporesponsive T Cells Facilitates a Th2 Bias in Peripheral Lymphocytes and at Materno-Fetal Interface in Murine Abortion-prone Matings. American Journal of Reproductive Immunology, 56: 258-266. doi: 10.1111/j.1600-0897.2006.00425.x ...
Aims Type 1 diabetes (T1D) is characterized by autoimmune depletion of insulin-producing pancreatic beta cells. and T cells of NOD mice. In macrophages, 12/15-LO deletion leads to decreased proinflammatory cytokine mRNA and protein levels. Furthermore, splenocytes from NOD-mice are unable Pecam1 to transfer diabetes in an adoptive transfer model. In islets, expression of 12/15-LO in …Read More. ...
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Cytokine and Chemokine analysis. □ Adoptive T cell therapy. □ Biomarkers. FACS analysis of Tumor Infiltrating Lymphocytes (TIL) in tumor tissues ...
It remains difficult to treat the multiplicity of distinct viral infections that afflict immunocompromised patients. Adoptive transfer of virus-specific T cells (VSTs) can be safe and effective, but such cells have been complex to prepare and limited in antiviral range. We now demonstrate the feasib …
We have initiated a tumor-infiltrating lymphocyte (TIL) therapy program that has now treated over 70 metastatic melanoma patients with an ongoing 45-50% clinica...
Post-Doc position in adoptive T cell immunotherapySummary:Adoptive transfer of gene-modified T cells induces cancer regression in heavily pretreated patients. Treatment involves ex-vivo engineering of autologous lymphocytes, to express a chimeric ant ...
BACKGROUND. Chimeric antigen receptor (CAR) T cells can induce remission in highly refractory leukemia and lymphoma subjects, yet the parameters for achieving sustained relapse-free survival are not fully delineated. METHODS. We analyzed 43 pediatric and young adult subjects participating in a phase I trial of defined composition CD19 CAR T cells (, NCT02028455). CAR T cell phenotype, function, and expansion, as well as starting material T cell repertoire, were analyzed in relationship to therapeutic outcome (defined as achieving complete remission within 63 days) and duration of leukemia-free survival and B cell aplasia. RESULTS. These analyses reveal that initial therapeutic failures (n = 5) were associated with attenuated CAR T cell expansion and/or rapid attrition of functional CAR effector cells following adoptive transfer. The CAR T products were similar in phenotype and function when compared with products resulting in sustained remissions. However, the initial apheresed ...
New blood cancer breakthroughs - including several involving CAR T cell therapy - were announced by City of Hopes cancer center physicians at the 2017 American Society of Hematology (ASH) annual meeting in Atlanta.
Immunotherapy is an attractive option to extend remission rates in ovarian cancer. The use of adoptive cell transfer (ACT) of ex vivo generated tumor-antigen sp...
Intent on streamlining the production of T-cell therapies, manufacturers are looking into flexible cleanrooms, modular facilities, dynamic reactor environments, and co-culture technologies.
Find out more about Car T-Cell Therapy, its pros and cons and how it will affect the market, also from a Pharmaceutical Consulting point of view.
Adoptive cell transfer (ACT) is the transfer of cells into a patient. The cells may have originated from the patient or from another individual. The cells are most commonly derived from the immune system with the goal of improving immune functionality and characteristics. In autologous cancer immunotherapy, T cells are extracted from the patient, genetically modified and cultured in vitro and returned to the same patient. Comparatively, allogeneic therapies involve cells isolated and expanded from a donor separate from the patient receiving the cells. In the 1960s, lymphocytes were discovered to be the mediators of allograft rejection in animals. Attempts to use T cells to treat transplanted murine tumors required cultivating and manipulating T cells in culture. Syngeneic lymphocytes were transferred from rodents heavily immunized against the tumor to inhibit growth of small established tumors, becoming the first example of ACT. Description of T cell growth factor interleukin-2 (IL-2) in 1976 ...
We have examined the migration of murine macrophages from the vascular compartment to normal and inflammatory tissues by the adoptive transfer of resident peritoneal macrophages (RPM phi) fluorescently labeled with the hydrophobic dye 1,1-dioctadecyl 3,3,3,3-tetramethylindocarbocyanine perchlorate (DiI). After initial labeling of the plasma membrane of RPM phi, the dye accumulated stably in intracellular vesicles of low density (rho = 1.042-1.045 kg/l) and cells remained viable in culture for 4 weeks. Like the normal monocyte, DiI-RPM phi, but not exudate-derived or fixed cells, migrated to peritoneal exudates, following i.v. adoptive transfer, by a mechanism inhibitable by an antibody to the type 3 complement receptor. In the absence of an inflammatory stimulus there was no migration to the peritoneal cavity, and DiI-RPM phi accumulated within 4 h in the red pulp and marginal zone of the spleen. By day 6 these cells still formed a tight ring of fluorescence in the marginal zone alone, ...
This dose-escalation study investigated the tolerability and efficacy of adoptive transfer of CH 296 (RetroNectin)-induced T-cell therapy in patients with
Adoptive cell therapy can be an effective treatment for some patients with advanced cancer. This report summarizes clinical trial results from the Surgery Branch, NCI, investigating tumor infiltrating lymphocytes (TIL) and gene engineered peripheral blood T cells for the therapy of patients with melanoma and other solid tumors.. Keywords: TIL, melanoma, NY-ESO-1, interleukin-2, lymphodepletion ...
Adoptive transfer of T cells genetically improved to sole anti-TSLPR chimeric antigen receptors can cure B-ALL in xenograft kinds. Philadelphia chromosomeClike (Ph-like) leukemias react badly to regular chemotherapy RICTOR and possess Angiotensin III (human, mouse) high prices of relapse.17 Multiple groupings have got Angiotensin III (human, mouse) now demonstrated that rearrangements accounts for fifty percent of Ph-like ALL genomic alterations and are also highly associated with concomitant and stage mutations.17,18,20,21,32 We keep that the TSLPR features as an ALL oncoprotein provided its cell surface area reflection and association with poor scientific outcomes and thus might be an ideal immunotherapeutic focus on. Furthermore, TSLPR phrase in regular tissue shows up to end up being limited. We demonstrate that a TSLPR CAR may eradicate individual Web site Angiotensin III (human, mouse) completely. For structure of the lengthy CAR constructs, the CH2CH3 websites from IGHG1 ...
Patients are being offered admission to this study to test the side effects of an investigational treatment prepared from special immune cells (T cells) specific for melanoma. A T-cell is a type of lymphocyte. Lymphocytes are a type of white blood cell that protect people from viral infections; help other cells fight bacterial and fungal infections; produce antibodies; fight cancers; and coordinate the activities of other cells in the immune system. These special immune cells will be taken from a sample of the patients tumor tissue that will be surgically removed from their body and grown in the laboratory. They will then given back to the patient in their veins. These cells are called tumor infiltrating lymphocytes (TIL). We wish to study the side effects of TIL when they are given with two chemotherapy drugs to temporarily decrease the patients own immune cells and a drug called Interleukin-2 (IL-2). The two chemotherapy drugs called fludarabine and cytoxan are used to greatly reduce the ...
OT could not be induced in CCR9−/− or β7−/− mice, or when MAdCAM-1 was blocked in wild-type mice, indicating that gut-homing receptors are required for oral tolerization. Consistent with the role of all-trans retinoic acid in inducing gut homing T cells, OT could not be induced in mice depleted of vitamin A. OT was rescued in CCR9−/− mice following adoptive transfer of wild-type T cells, but not CCR9−/− or β7−/− T cells. Gut-homing T cells are therefore necessary and sufficient to induce OT. Wild-type TREG cells and IL-10 were required to restore OT to CCR9−/− mice, indicating that homing and functional differentiation of IL-10-producing TREG cells in the gut is required for OT. Conversely, transfer of CCR9−/− or β7−/− T cells to wild-type mice partially inhibited OT ...
Principal Investigator:ICHIDA Takafumi, Project Period (FY):2002 - 2003, Research Category:Grant-in-Aid for Scientific Research (C), Section:一般, Research Field:Gastroenterology
Hahm E, Wei C, Fernandez I, Li J, Tardi NJ, Tracy M, Wadhwani S, Cao Y, Peev V, Zloza A, Lusciks J, Hayek SS, OConnor C, Bitzer M, Gupta V, Sever S, Sykes DB, Scadden DT, Reiser J. Bone marrow-derived immature myeloid cells are a main source of circulating suPAR contributing to proteinuric kidney disease. Nat Med. 2017 01; 23(1):100-106 ...
Words starting with A (page 22): adoptionism, adoptionist, adoptions, adoptious, adoptive, adoptively, adopts, adorabilities, adorability, adorable, adorableness, adorably, adoral, adoration, adore, adored, adorer, adores, adoring, adoringly...
Lymph node activation is an intrinsic aspect of T cell activation by infectious pathogens and vaccines. We separated the T cell priming event from lymph node activation in space and time by exploiting an experimental model consisting of TCR-transgenic OT-I CD8+ T cells and engineered APCs expressing high levels of the cognate OVA-derived peptide epitope and the costimulatory ligand CD80. Prior work had demonstrated that a 20-h in vitro encounter with these APCs empowered naive OT-I cells to vigorously proliferate in vivo upon transfer to naive syngeneic recipient (15). Furthermore, these T cells were shown to develop into long-lived memory cells, capable of clonal expansion and protective effector function in response to secondary Ag encounter (16). Importantly, progression of the 20 h primed OT-I T cells through primary expansion, contraction, and memory phases required neither in vivo exposure to Ag nor the context of an inflamed, congested lymph node. This model therefore offered a unique ...
We explored the function of endogenous type I IFNs (IFN-1) in the colon using the T cell adoptive transfer model of colitis. Colon mononuclear phagocytes (MPs) constitutively produced IFN-1 in a Toll/IL-1R domain-containing adapter-inducing IFN-β-dependent manner. Transfer of CD4(+)CD45RB(hi) T cells from wild-type (WT) or IFN-α/β receptor subunit 1 knockout (IFNAR1(-/-)) mice into RAG(-/-) hosts resulted in similar onset and severity of colitis. In contrast, RAG(-/-) × IFNAR1(-/-) double knockout (DKO) mice developed accelerated severe colitis compared with RAG(-/-) hosts when transferred with WT CD4(+)CD45RB(hi) T cells. IFNAR signaling on host hematopoietic cells was required to delay colitis development. MPs isolated from the colon lamina propria of IFNAR1(-/-) mice produced less IL-10, IL-1R antagonist, and IL-27 compared with WT MPs. Accelerated colitis development in DKO mice was characterized by early T cell proliferation and accumulation of CD11b(+)CD103(-) dendritic cells in the mesenteric
Adoptive Transfer of Dendritic Cells Expressing Fas Ligand Modulates Intestinal Inflammation in a Model of Inflammatory Bowel Disease Abstract.
In recent years, the search for new cancer treatments has increasingly focused on immunotherapies that harness the bodys own defenses to fight tumors. Adoptive cell therapy (ACT) is a powerful immunotherapeutic strategy that can effectively control some cancers but that also has drawbacks.
The key component of adoptive T cell therapy is generation of functional tumor-specific T cells without immune tolerance to self-antigens. This can be accomplished by genetic modification of patient T cells using genes encoding CARs. Recently, the third-generation of CAR T cells has been developed, which includes an additional co-stimulatory domain, such as CD28 plus 4-1BB or CD28 plus OX40 together with CD3zeta along with the specific target. Results from several clinical trials in different settings including CAR design, culture techniques, lymphodepleting strategies, and target diseases, have provided valuable insights, and CAR T cells are emerging as a powerful therapy in hematologic malignancies [6]. Most clinical successes of CAR T cell therapy are recorded in the setting of B-cell malignancies, by targeting CD19 (Table 1). Several B cell antigens, such as CD20, CD22, CD23, and CD38 are under evaluation as alternative CAR T cell targets in B-cell malignancies. However, targets that are ...
Chemotherapy and IL-2 plus T-cells and the vaccine of dendritic cells received by vein (IV) about 4 hours after T-cells and again on Day 21 (+/- 7 days). Cyclophosphamide 60 mg/kg/d IV over 2 hours Days -7 and -6 (with Mesna) and Fludarabine 25 mg/m^2 IV daily Days -5 to -1 before T cell infusion. On Day 0, up to 1.5 x 10^11 T cells IV infusion over 30-60 minutes. Interleukin-2 12-16 hours after T cell infusion at standard dose of 720,000 IU/kg as intravenous bolus over 15 minute period every 8-16 hours for up to 15 doses on Days 1-5 and 22-26 ...
The data presented show that IFN-I and their signal transducer STAT-1 essentially contribute to the induction of OVA-specific CTL following immunization with OVA peptide and IC31 immune adjuvant. Surprisingly and contrasting many experimental situations with pathogen-specific CTL, OVA-specific CTL did not require IFN-I/STAT-1 as an activation/survival signal. This was shown by the use of conditional IFNAR1 deletion and by adoptive transfer of wt or STAT-1-mutant OT-1 CD8+ T cells into STAT-1-deficient animals. By contrast, adoptive transfer experiments with myeloid DC showed that IFN-I/STAT-1 are required to render these cells fully capable of stimulating CD8+ T cell activation. The IFN-I/STAT-1 effect most likely results from the ability to stimulate DC maturation and activation (35, 36, 37, 38). The reported stimulation of cross-presentation by DC may account for the stronger effect of IFN-I/STAT-1 in vivo when compared with our in vitro experiments, because immunization with the OVA/IC31 ...
TY - JOUR. T1 - Adoptive transfer of zoledronate-expanded autologous V 39Vδ 2 T-cells in patients with treatment-refractory non-small-cell lung cancer. T2 - A multicenter, open-label, single-arm, phase 2 study. AU - Kakimi, Kazuhiro. AU - Matsushita, Hirokazu. AU - Masuzawa, Keita. AU - Karasaki, Takahiro. AU - Kobayashi, Yukari. AU - Nagaoka, Koji. AU - Hosoi, Akihiro. AU - Ikemura, Shinnosuke. AU - Kitano, Kentaro. AU - Kawada, Ichiro. AU - Manabe, Tadashi. AU - Takehara, Tomohiro. AU - Ebisudani, Toshiaki. AU - Nagayama, Kazuhiro. AU - Nakamura, Yukio. AU - Suzuki, Ryuji. AU - Yasuda, Hiroyuki. AU - Sato, Masaaki. AU - Soejima, Kenzo. AU - Nakajima, Jun. PY - 2020/9/18. Y1 - 2020/9/18. N2 - Background Not all non-small cell lung cancer (NSCLC) patients possess drug-targetable driver mutations, and response rates to immune checkpoint blockade therapies also remain unsatisfactory. Therefore, more effective treatments are still needed. Here, we report the results of a phase 2 clinical trial of ...
Wilms tumor 1 antigen (WT1) is definitely overexpressed in acute myeloid leukemia (AML) a high-risk neoplasm warranting advancement of book immunotherapeutic strategies. (CTLs) demonstrated antigen-specific reactivity against WT1 and against WT1+ leukemia cells. SmartDC/tWT1 injected s.c. into Nod.Rag1?/?.IL2rγc?/? mice were viable for more than three weeks. Migration of human being T cells (huCTLs) to the immunization site was shown following adoptive transfer of huCTLs into mice immunized with SmartDC/tWT1. Furthermore SmartDC/tWT1 immunization plus adoptive transfer of T cells reactive against WT1 into mice resulted in growth arrest of a WT1+ tumor. Gene array analyses of SmartDC/tWT1 proven upregulation of several genes related to innate immunity. Therefore SmartDC/tWT1 can be produced in a single day time of gene transfer are highly viable culture methods or by gene transfer VER-50589 of transgenic T-cell receptors for adoptive immunotherapy (Ho are usually quiescent which may hamper ...
The Ivan Borrello Lab focuses on the development of a novel approach of adoptive T cell therapy utilizing marrow-infiltrating lymphocytes (MILs) as a more tumor-specific T cell approach. This has led to establishing the first adoptive T cell trials at Johns Hopkins and an exploration of this approach in other diseases, including nonhematologic malignancies. The lab also examines strategies for treating minimal residual disease (MRD) in myeloma with the combination of immune modulation and whole cell-based vaccines.. Research Areas: immunology, vaccines, multiple myeloma, cancer, translational research, immunotherapy, T cells ...
Further verification of a graft-vs-tumor effect came from efforts to treat patients for posttransplant leukemic recurrence by infusing viable donor lymphocytes, resulting in sustained complete remissions in most patients with chronic myelogenous leukemia and in some patients with other hematologic malignancies.11 By the mid-1980s, it was generally accepted that adoptive transfer of allogeneic T cells could be of benefit, but was of limited potency, sometimes associated with significant toxicity in the form of graft-vs-host disease and restricted in its application to the post-allogeneic transplant setting.12 To make adoptive cell therapy more effective and broadly applicable, a large number of challenges presented themselves, including the requirement to be able to clone and expand tumor-reactive T cells, to define conditions that allow for their expansion and survival after reinfusion, to identify appropriate tumor-associated targets, and to develop methods to genetically engineer T cells to ...
In the May 5, 2017, issue of Science Immunology, cancer researchers at the Medical University of South Carolina report that blood platelets blunt the immune response to cancer. Genetic inactivation of platelets improved the ability of T cells to fight melanoma in preclinical tests. Adoptive T cell therapies for cancer could be enhanced when combined with common antiplatelet drugs.
The medical centers listed below currently offer Chimeric Antigen Receptor T-cell (CAR T-cell) therapy that has been approved by the U.S. Food and Drug Administration (FDA). Each center has been carefully trained and certified to offer this new treatment.. These, and other medical centers, may also be testing other types of CAR T-cell therapy that are not yet approved by the FDA, but which may be available to you if you participate in a clinical trial. To learn more about CAR T-cell therapies currently being studied in a clinical trial click here [1].. ...
This page includes the following topics and synonyms: CAR T-Cell Therapy, CAR T Cell Therapy, Chimeric Antigen Receptor T Cell Therapy, Cytokine Release Syndrome, Tisagenlecleucel, Axicabtagene ciloleucel.
Twist Bioscience and Neogene Announce Broad Strategic Partnership for Next Generation Personalized T Cell Therapies - read this article along with other careers information, tips and advice on BioSpace
Passive transfer of DENV2 E85-VRP-immune serum or adoptive transfer of DENV2 E85-VRP-immune B cells can increase the viral RNA levels in the liver upon infectio
Eureka Therapeutics announced FDA allowance of its IND application to commence a phase I clinical trial for ET190L1-ARTEMIS T cells.
Eureka Therapeutics announced FDA allowance of its IND application to commence a phase I clinical trial for ET190L1-ARTEMIS T cells.
Unsure if you should select CAR T-Cell Therapy for your CLL? A CLL patient who was diagnosed with aggressive CLL shares his treatment journey and experience participating in a CAR T-cell clinical trial.
The side effects of CAR-T cell therapy manifest a few weeks after the therapy, letting the body adjust to new changes which can be managed with medication.
Stiprinošās lentas - Lentes - Ķīmiski preparāti - Darbnīcas aprīkojumi - Plašs produktu piedāvājums Transfer Multisort Elektronik firmā.
Rajesh's adoptive daughter; Rishabh and Geetanjali's sister-in-law and adoptive sister; Rajveer's childhood friend; Abhimanyu, ... Rajveer bids adieu saying he got transferred. Geetanjali and Abhimanyu reunite forever and start living happily ever after. ... Kavya's adoptive sister; Rajveer's childhood friend; Abhimanyu's wife; Ragini, Aditya and Kavya's sister-in-law; Varun's aunt ... Kavya's adoptive brother; Ragini's husband; Abhimanyu, Aditya and Kavya's brother-in-law; Varun's father Nivedita Bhattacharya ...
Radha is brought up by her adoptive family. When she was young, her father, a famous singer, died in a car accident. ... Thangapandian, an honest forest officer, is transferred to a village. The village chief cuts illegally the sandalwood trees. ...
Approaches include antibodies, checkpoint therapy, and adoptive cell transfer. Laser therapy uses high-intensity light to treat ...
Ongoing research is looking at treatment by adoptive cell transfer. Adoptive cell transfer refers to the application of pre- ... The second treatment, adoptive transfer of genetically altered autologous lymphocytes, depends on delivering genes that encode ... Hershkovitz L, Schachter J, Treves AJ, Besser MJ (2010). "Focus on adoptive T cell transfer trials in melanoma". Clinical & ... May 2010). "Clinical responses in a phase II study using adoptive transfer of short-term cultured tumor infiltration ...
Later in the series, Jun is revealed to be Kyōko's adoptive brother, as her mother adopted him as her own son after his parents ... She is a middle school student who recently transferred to Yokohama. She is a budding fashion designer and a fan of Prism Stone ... She is also Kyōko's mother, and Jun's adoptive mother. She divorced Kyōko's father because of his obsession with the Grateful ... Kaname's adoptive father. He once wanted to become a superstar, which is reflected in his current attire. Because of Sonata's ...
Clinical responses to adoptive transfer of T cells were observed in patients with metastatic melanoma resistant to multiple ... Adoptive cell transfer in vitro cultivates autologous, extracted T cells for later transfusion. Alternatively, Genetically ... Rosenberg SA, Restifo NP, Yang JC, Morgan RA, Dudley ME (April 2008). "Adoptive cell transfer: a clinical path to effective ... October 2012). "Efficacy of adoptive cell transfer of tumor-infiltrating lymphocytes after lymphopenia induction for metastatic ...
He is adoptive brother of Silvia Süller. Carluccio, José (2011-10-29). "Argentina: 1ra. "C" AFA 1988/89 - Torneo Octogonal" (in ... He returned to Argentina as he transferred to All Boys in 1992 and played 16 games for the club. Between 1993 and 2000 he ...
Marie Parry - Thomas's adoptive sister, whom he has a crush on. Mr Vishram - Thomas's boss at the Department of Transfer and ... Thomas Parry - protagonist Victor Parry - Thomas's adoptive father, a railroad engineer for the Red Quarter. ...
In Adoptive T cell transfer therapy, TILs are expanded ex vivo from surgically resected tumors that have been cut into small ... The use of TILs as an adoptive cell transfer therapy to treat cancer was pioneered by Dr. Steven Rosenberg and colleagues at ... Dudley ME, Rosenberg SA (September 2003). "Adoptive-cell-transfer therapy for the treatment of patients with cancer". Nature ... "Efficacy of adoptive cell transfer of tumor-infiltrating lymphocytes after lymphopenia induction for metastatic melanoma". ...
season 2) Christina Marie Karis as Kari Bennett, the adoptive mother of Iris and Hope, who was killed by Amelia at the ... Hal Cumpston as Silas Plaskett, a shy teenager who was transferred to the Campus Colony after committing a violent offense. ... Alexa Mansour as Hope Bennett, Iris' adoptive, rebellious and intellectual sister. ... the adoptive father of Iris and Hope, who is a scientist that is being held by the CRM. (season 2; recurring season 1) Natalie ...
"Erica Parsons' adoptive father released from federal custody, transferred to state prison". Salisbury Post. January 20, 2022. ... He said he had not seen his adoptive sister in almost two years. He told police that Erica had been abused, and that he ... Jamie testified that Erica's adoptive father, Sandy, would often get mad and punch Erica on her head. Soon after Erica was ... Federal prosecutor Anand Ramaswamy said there was no record of Erica during the time her adoptive parents illegally accepted ...
2005). "Adoptive transfer of cytomegalovirus-specific CTL to stem cell transplant patients after selection by HLA-peptide ... "Multimer technologies for detection and adoptive transfer of antigen-specific T cells". Cancer Immunol Immunother. 2010 (59): ... Uhlin; Okas M; Gertow J; Uzunel M; Brismar TB; Mattsson J. (2009). "A novel haplo-identical adoptive CTL therapy as a treatment ... 2009 a special dispensation was granted for a team to use them for isolating EBV-specific T cells for mother-daughter transfer ...
Adoptive T cell therapy is a form of passive immunization by the transfusion of T-cells (adoptive cell transfer). They are ... Another approach is adoptive transfer of haploidentical γδ T cells or NK cells from a healthy donor. The major advantage of ... "Successful adoptive transfer and in vivo expansion of haploidentical γδ T cells". Journal of Translational Medicine. 12: 45. ... The first 2 adoptive T-cell therapies, tisagenlecleucel and axicabtagene ciloleucel, were approved by the FDA in 2017. ...
Adoptive transfer of TILs can increase the survival of melanoma patients when it is used as an adjuvant therapy, i.e. after a ... Adoptive transfer involves either collecting from patients intratumoral or blood T cells, stimulate them in vitro against ... Adoptive TIL transfer in the adjuvant setting for melanoma: long-term patient survival ". Journal of Immunology Research. 2014 ... but also the adoptive transfer of T cells that recognize these antigens. Finally, antibodies that increase the general activity ...
Once all parties agree, the embryo is transferred to the adoptive mother's clinic for a frozen embryo transfer.[citation needed ... Embryo ownership is transferred directly from the genetic parents to the adoptive parents. Genetic parents may be updated by ... is a misnomer because the transfer of an embryo is handled as property transfer. Abortion rights advocates, advocates of ... These scientific advances set the stage to allow open and candid discussion of embryo donation and transfer as a solution to ...
Tisagenlecleucel is an adoptive cell transfer therapy for B-cell acute lymphoblastic leukemia; T cells from a person with ... The goal was to cure malignant brain tumors by using recombinant DNA to transfer a gene making the tumor cells sensitive to a ... Baum C, Düllmann J, Li Z, Fehse B, Meyer J, Williams DA, von Kalle C (March 2003). "Side effects of retroviral gene transfer ... The first therapeutic use of gene transfer as well as the first direct insertion of human DNA into the nuclear genome was ...
On 7 January 2008, he became English Premier League side Portsmouth F.C.'s first signing in the 2008 January transfer window. ... Danijel Subotić began his career at FC Basel in his adoptive country Switzerland. ...
"Recipient T Cell Exhaustion and Successful Adoptive Transfer of Haploidentical Natural Killer Cells". Biology of Blood and ... It was also shown that recipient T cell exhaustion provides sufficient conditions for NK cell transfer. While there are data ...
May 1995). "Prolonged disease-free period in patients with advanced epithelial ovarian cancer after adoptive transfer of tumor- ... Adoptive Immuno cell therapy of cancer was first introduced by Steven Rosenberg and his colleagues of National Institute of ... September 2000). "Adoptive immunotherapy to lower postsurgical recurrence rates of hepatocellular carcinoma: a randomised trial ... July 2003). "Tumor-localization by adoptively transferred, interleukin-2-activated NK cells leads to destruction of well- ...
and was also verified for HIV in the mouse model by adoptive transfer experiments. Non-human primate experiments also indicate ... Thus, the approach has also transferred well for the treatment of hepatitis B and HIV. One of the approaches for a protective ...
The adoptive transfer of tumor-specific effector T cells knocked out or knocked down for CISH resulted in a significant ... CISH knock out in T cells increased PD-1 expression and the adoptive transfer of CISH knock out T cells synergistically ... Adoptive cell transfer Cancer immunotherapy Chimeric antigen receptor Immune checkpoint Pardoll DM (March 2012). "The blockade ... Other modes of enhancing [adoptive] immunotherapy include targeting so-called intrinsic checkpoint blockades. Many of these ...
The adoptive parents hired an Illinois attorney, Denise Patton, to arrange the transfer of custody. The child was born on June ... After the adoptive parents filed for adoption in South Carolina, the biological father learned of the prospective adoption and ... Godwin was the attorney for the adoptive couple, not for the birth mother. Godwin said the US Supreme Court decision, which ... In this case, the prospective adoptive parents, Bobby and Diane Bixler, removed the child from Oklahoma without the Interstate ...
"Multifunctional T cell analyses to study response and progression in adoptive cell transfer immunotherapy". Cancer Discovery. 3 ... Economou's research interests in tumor immunology include dendritic cell vaccination, DNA vaccines and adoptive cell therapy ...
"Reversible MHC multimer staining for functional isolation of T-cell populations and effective adoptive transfer". Nat Med. 8 (6 ...
Around age 20, while he was in college, he lost his adoptive mother to cancer. His father quit his job and moved to Indiana to ... Clement stopped going to college in Massachusetts and transferred to Purdue University in Indiana. Eventually, he got tired of ... his Electronical Engineering program and switched to Psychology instead and transferred to Indiana University. He finished his ...
"Restoration of Viral Immunity in Immunodeficient Humans by the Adoptive Transfer of T Cell Clones". Science. 257 (5067): 238- ... His research is focused on T cell adoptive immune therapy. His group demonstrated the potential of isolating antigen-specific T ... "Reconstitution of Cellular Immunity against Cytomegalovirus in Recipients of Allogeneic Bone Marrow by Transfer of T-Cell ...
Adoptive cell transfer therapy following non-myeloablative but lymphodepleting chemotherapy for the treatment of patients with ... Restoration of immunity in lymphopenic individuals with cancer by vaccination and adoptive T-cell transfer. Nat Med 2005;11(11 ... Adoptive Cell Therapy for Patients With Metastatic Melanoma: Evaluation of Intensive Myeloablative Chemoradiation Preparative ... The latter situation -lymphodepletion- has been studied extensively and the proliferation of mature T cells upon transfer into ...
"Adoptive transfer of T-cell precursors enhances T-cell reconstitution after allogeneic hematopoietic stem cell transplantation ...
He transferred his patrimony to his adoptive son, Date Muneoki (伊達 宗興) and retired. But he returned to the Sonnō jōi movement ... and he became an adoptive son of his uncle, Date Moriaki (伊達 盛明). He inherited a patrimony by 12 years old, and was appointed " ...
ShK, ShK-Dap22, ShK-170 and PEGylated ShK-Q16K prevent adoptive-transfer EAE in Lewis rats, a model of multiple sclerosis. ... PEGylated ShK[Q16K] prevented adoptive-transfer experimental autoimmune encephalomyelitis in rats, a model for multiple ...
Mahapajapati Gotamī (aunt, adoptive mother). *Yaśodharā (wife). *Rāhula (son). *Ānanda (cousin). *Devadatta (cousin) ...
Zhu transferred Han to Pinglu Circuit (平盧, headquartered in modern Weifang, Shandong) and transferred the military governor of ... Meanwhile, Han sent threats to Li Maozhen's adoptive son Li Jitang (李繼瑭), who was then the military governor of Kuangguo, and ... They also forced Emperor Zhaozong to issue an edict transferring Wang Ke to Kuangguo, Wang Gong to Hezhong, and Wang Xingyue to ... Yang fled to Yang Fuguang's adoptive son Yang Shouliang the military governor of Shannan West, and they, along with Yang ...
Adoptive transfer of T cells expressing CARs is a promising anti-cancer therapeutic, because CAR-modified T cells can be ... "Safe engineering of CAR T cells for adoptive cell therapy of cancer using long-term episomal gene transfer". EMBO Molecular ... Bonini C, Ferrari G (1997). "HSV-TK gene transfer into donor lymphocytes for control of allogeneic graft-versus-leukemia". ... allowing adoptively transferred T cells to target HIV infected cells, although it failed to show any clinical improvement.[15] ...
His adoptive father was a hard-working coal miner, but the family still had trouble making ends meet. Gavin quit school after ... The 82nd would stay in the Netherlands until November 13, when it was transferred to new billets in Sisonne et Suippes, France ... Knowing that his adoptive parents would not consent, Gavin told the recruiter he was an orphan. The recruiter took him and a ...
The one exception to passive humoral immunity is the passive transfer of cell-mediated immunity, also called adoptive ... Passive immunity is the transfer of active humoral immunity of ready-made antibodies. Passive immunity can occur naturally, ... This condition is referred to as failure of passive transfer. It can be diagnosed by measuring the amount of IgG in a newborn's ... Passive immunity is also provided through colostrum and breast milk, which contain IgA antibodies that are transferred to the ...
The relatives on the mother's side and father's side are equally important for emotional ties or for transfer of property or ... blended families would also become problematic as the activities that were once performed in the old family may not transfer ...
Diocletian tried to solve this problem by re-establishing an adoptive succession with a senior (Augustus) and junior (Caesar) ... He now felt that he could dispense with Alaric's services and he nominally transferred Alaric's province to the West. This ...
Recently, phage display has also been used in the context of cancer treatments - such as the adoptive cell transfer approach. ...
These transfers were restored by Lobster Films of France, but inexplicably The Painted Desert is still in its truncated form, ... Years later the baby has now grown into a young man, Bill Holbrook (William Boyd), who works with his adoptive father on their ...
... while Emperor Zhuangzong's adoptive brother Li Siyuan had recently launched a successful surprise attack against Later Liang's ... and therefore tried to resign the chief of staff post and have it transferred to Li Shaohong, but Emperor Zhuangzong did not ...
He lived with his adoptive parents and older sister, Hung Tzu-yung. Hung attended the National Cheng Kung University, where he ... Hung was initially sent to Ten-Chen Medical Hospital, but was subsequently transferred to Tri-Service General Hospital when his ... The military investigation has led to the arrest and questioning of several officials, with the case eventually transferred to ... which included the abolition of court martial during peacetime and transfer of military prisoners to civilian prisons. Members ...
Gjerdingen DK, Froberg DG (January 1991). "The fourth stage of labor: the health of birth mothers and adoptive mothers at six- ... Loftus JR, Hill H, Cohen SE (August 1995). "Placental transfer and neonatal effects of epidural sufentanil and fentanyl ... "the process of childbirth is not finished until the baby has safely transferred from placental to mammary nutrition." It is ...
Peters stated of the film, "I have learned that he's my father at this point ... It's like an adoptive child or any kind of ... Following The Walt Disney Company's purchase of 21st Century Fox, all X-Men-related characters were transferred back to Marvel ...
Snatcher reveals that the Guild has been transferring the size of captured Underlings to "the Great One", and feeding him with ... Protagonist Arthur lives with his adoptive grandfather, William, in the complex network of tunnels beneath Ratbridge, where ... Still believing her a boxtroll, the Guild transfers Marjorie's size to the rat, leaving her only seven inches tall. Arthur is ...
He is the adoptive father of Max after his father died. Being an international counter-terrorism agency, N-Tek answers to the ... During the season 2 finale (S2E13 - "Breakout"), Psycho and Bio-Con break him out while he is being transferred to another ... working for his adoptive father, Jefferson Smith of N-Tek, which supplies such equipment. He later discovers that N-Tek is a ...
The robbers then took $1.6 million in cash and raced to transfer this into the waiting U-Haul. The truck was soon stopped by a ... was raised in Chicago by adoptive parents Bill Ayers and Bernardine Dohrn, who, like his parents, had been members of the ... more successful project in Great Meadows Prison in Comstock following his transfer to the eastern part of the state.[citation ...
Bittaker discovered that his adoptive parents had disowned him and moved to another state. He would never see his adoptive ... He was later transferred to the medical center for federal prisoners in Springfield, Missouri, to serve the remainder of his ... Bittaker's adoptive father worked in the aviation industry, which required the family to frequently move around the United ... He died of natural causes at the California Medical Facility on February 24, 2020, at the age of 72, having been transferred to ...
Sharon is the adoptive daughter of the popular 'supercouple'; Den and Angie. She was known to Den as his "princess" and he made ... The following month Rich is transferred to another division in Crawley, so Debbie decides to leave Walford with him. Tony ... On 14 April 2003, Tracey has a one-night stand with Dennis Rickman (Nigel Harman), the adoptive half-brother of former pub ...
However, after a transfer from Modern Times Group to Sony, the broadcast was ceased. In the United States, all episodes are ... Ryan Eggold as Tom Keen (seasons 1-5): Born Christopher Hargrave, later taking the adoptive name Jacob Phelps, Keen works as a ... In Italy, the first seven seasons and the first seventeen episodes of season 8 were broadcast on Fox Crime before a transfer to ... and Homeland due to the mutual connection between the characters of Reddington and Nicholas Brody and the transfer of Diego ...
51-308). Transfer of child charged with a felony to the regular criminal docket". Justia Law. Retrieved October 9, 2019. "2005 ... The offender is the other person's natural or adoptive parent, or a stepparent, or guardian, custodian, or person in loco ...
Poe iterated that she will continue and enhance the Conditional Cash Transfer Program (Pantawid Pamilyang Pilipino) or 4Ps and ... Sheryl Cruz, actress and adoptive cousin of Poe, withdrawn her support to the Senator's presidential campaign recently. Cruz ... Grace Poe Family members Neil Llamanzares, husband Brian Poe, son, CNN Philippines reporter Lovi Poe, adoptive half-sister ... Susan Roces, adoptive mother Presidents of the Philippines Joseph Estrada, 13th President of the Philippines (1998-2001) and ...
Children on their own would not survive in the camp but women would step forward and behave as surrogate/adoptive mothers, ... SOE agents who survived were Yvonne Baseden and Eileen Nearne, who was a prisoner in 1944 before being transferred to another ... Approximately 500 women from Ravensbrück were transferred to Dachau, where they were assigned as labourers to the Agfa-Commando ...
She is the adoptive daughter of Wu Shifu, learning kung fu and values under him in order to become the Guardian of the Sacred ... However, he is overpowered, so he transfers the title of Guardian to Marinette, losing all his memories in the process. He ...
They had two adoptive children, son (Simon d. 1981) and a daughter (Byrony). Akers-Jones died from colorectal cancer at Queen ... In the years leading up to the transfer of sovereignty from the UK to the People's Republic of China (PRC) in 1997, Akers-Jones ...
An unknown entity answered their prayer and though they briefly saw Bee's spirit, the entity convinced them to transfer its ... Twelve years later, after being involved in devil worship, a grown-up Annabelle returns home and murders her adoptive parents, ...
However, this transfer of power within the family seems to have been smooth, and it is likely that Arsames was still the ... and interviewed him and his adoptive father. Upon the shepherd's confession, Astyages sent Cyrus back to Persia to live with ...
Back in Springfield, Alan hired Bradley with the intent of transferring him out of town in order to separate Phillip and Beth. ... who was in search of her real mother now that her adoptive mother had died. In 1999, after telling Harley and Phillip her ... Although Phillip arranged for him to be transferred to a rehabilitation clinic, he could not be persuaded by Alan to stay in ...
Gladney then arranged the transfer of the children to the Texas Children's Home and Aid Society in Fort Worth run by Reverend I ... She helped to grant adoptive children the same rights as "natural" children and gave orphaned children and many birth mothers a ... She continued the work of Reverend Morris by placing abandoned children with adoptive families. She also expanded the society's ... Gladney placed over 10,000 babies with adoptive parents during her career and totally revolutionized adoption practices. ...
Marshall and his regiment were transferred to Andrew Jackson Smith's command in Missouri and took part in the pursuit of ... his adoptive state experienced a post-Civil-War surge of growth and development: its population doubled to 350,000, its ... He continued in brigade command when transferred to Mobile, Alabama to take part in the Battle of Fort Blakeley. Colonel ...
After her commander tested her shooting skills he promoted her to the rank of Senior Sergant allowed her to be transferred to ... During the Great Purge her adoptive uncle was arrested. Before his arrest he had encouraged Mametova to study medicine. After ...
Adoptive cell transfer (ACT) is the transfer of cells into a patient. The cells may have originated from the patient or from ... Adoptive Transfer at the US National Library of Medicine Medical Subject Headings (MeSH) Adoptive Immunotherapy at the US ... The adoptive transfer of tumor-specific effector T cells knocked out or knocked down for CISH resulted in a significant ... Marcus A, Eshhar Z (2011). "Allogeneic adoptive cell transfer therapy as a potent universal treatment for cancer". Oncotarget. ...
Preliminary studies on artificial immunization against Plasmodium berghei and adoptive transfer of this immunity by splenic T ... 1976)‎. Preliminary studies on artificial immunization against Plasmodium berghei and adoptive transfer of this immunity by ...
Deprez, F; Zattoni, M; Mura, M L; Frei, K; Fritschy, J M (2011). Adoptive transfer of T lymphocytes in immunodeficient mice ... Adoptive transfer of T lymphocytes in immunodeficient mice influences epileptogenesis and neurodegeneration in a model of ... Download PDF Adoptive transfer of T lymphocytes in immunodeficient mice influences epileptogenesis and neurodegeneration in a ... 31, 4037). Here, we investigated the specific role of adaptive immunity in this TLE model by adoptive i.v. transfer of ...
A mouse model of pemphigus vulgaris by adoptive transfer of naive splenocytes from desmoglein 3 knockout mice」の研究トピックを掘り下げます。これ ... A mouse model of pemphigus vulgaris by adoptive transfer of naive splenocytes from desmoglein 3 knockout mice. / Aoki-Ota, M.; ... A mouse model of pemphigus vulgaris by adoptive transfer of naive splenocytes from desmoglein 3 knockout mice. British Journal ... A mouse model of pemphigus vulgaris by adoptive transfer of naive splenocytes from desmoglein 3 knockout mice. In: British ...
Adoptive cell transfer: a clinical path to effective cancer immunotherapy. Nat Rev Cancer. 2008 Apr. 8(4):299-308. [QxMD ... Adoptive T-cell transfer was administered, with peptide-pulsed dendritic cell (DC) vaccines undertaken during the period of ... Neither administration of the DCs nor adoptive transfer of autologous natural killer T cells activated with αGalCer and IL-2 ... 14] This platform has been shown in a preclinical model to enhance the effectiveness of adoptively transferred CD8+ T cells in ...
Adoptive cell transfer: a clinical path to effective cancer immunotherapy. Nat Rev Cancer. 2008 Apr. 8(4):299-308. [QxMD ... Adoptive T-cell transfer was administered, with peptide-pulsed dendritic cell (DC) vaccines undertaken during the period of ... Neither administration of the DCs nor adoptive transfer of autologous natural killer T cells activated with αGalCer and IL-2 ... 14] This platform has been shown in a preclinical model to enhance the effectiveness of adoptively transferred CD8+ T cells in ...
Cell Labeling and Adoptive Transfer. For adoptive transfer, CD44hiCD62L− CD4+ T, naive CD44loCD62L+ CD4+ T, and naive B cells ... We could show this by adoptive transfer of CD44hiCD62L− T helper cells from BM. Within 1 day after transfer, 4% of CD44hiCD62L− ... To monitor transferred cells in host mice, cells were labeled before transfer with 1 μM of Carboxyfluorescein diacetate ... the latter after adoptive transfer of CD44hiCD62L− CD4+ T cells from BM or spleen into C57BL/6 mice. This professional help was ...
title = "Adoptive Cell Transfer Therapy",. abstract = "Adoptive cell transfer therapy has developed into a potent and effective ... Adoptive Cell Transfer Therapy. / Dudley, Mark E.; Rosenberg, Steven A.. In: Seminars in oncology, Vol. 34, No. 6, 12.2007, p. ... Dudley, Mark E. ; Rosenberg, Steven A. / Adoptive Cell Transfer Therapy. In: Seminars in oncology. 2007 ; Vol. 34, No. 6. pp. ... N2 - Adoptive cell transfer therapy has developed into a potent and effective treatment for patients with metastatic melanoma. ...
Adoptive cell transfer. How it works. This experimental new avenue uses white blood cells called tumor-infiltrating lymphocytes ...
Categories: Adoptive Transfer Image Types: Photo, Illustrations, Video, Color, Black&White, PublicDomain, CopyrightRestricted 7 ...
Adoptive cell transfers. A total of 5 × 105 Smarta or 3 × 105 P14 cells were either left untreated or stained with CellTrace ... Therefore, we performed adoptive transfers of CellTrace-labeled T cells from P14_CD90.1 mice in which most CD8 T cells express ... 5A, 5B). We further analyzed early T cell priming by adoptive transfer of CellTrace-labeled T cells from Smarta_CD90.1 mice in ... F) Activation status and IFN-γ production of Smarta T cells at day 29 after transfer and infection in the spleen. Contour plots ...
High levels of hEPO were detected in the serum of adoptively transferred normal mice after PE administration, and this ... were adoptively transferred to normal or mutant (EPO-deficient) hosts. ... Adoptive Transfer * Anemia / immunology * Anemia / therapy * Animals * B-Lymphocytes / immunology* * Drug Delivery Systems ...
Figure 3. Adoptive transfer of GFP+ monocytes in CD45.2 recipient mice. One million GFP+ monocytes were adoptively transferred ... Adoptive Transfer of Monocytes Sorted from Bone Marrow. 骨髓源性单核细胞的过继转移 Damya Laoui Eva Van Overmeire. Chloé Abels. Jiri Keirsse ... Keywords: Adoptive transfer (过继转移), Monocytes (单核细胞), Pre-cDCs (Pre-cDCs), Tumor-associated macrophages (肿瘤相关巨噬细胞), Tumor- ... Figure 2. Adoptive transfer of CellTrace-labeled CD45.1+ monocytes in CD45.2 recipient mice. One
"Targeted Cancer Nanotherapeutics by Adoptive Transfer of Mononuclear Splenocytes." (2014). ...
Maryland Transfer Form SR7 if transferring from a Maryland Public School. *Copy of last report card/high school transcript ...
However, additional adoptive transfer of MDSCs did not further ameliorate corneal survival in these allogeneic corneal ... Strikingly, iMDSC transferring significantly reduced neovascularization that was comparable to transferring of tMDSCs, without ... Myeloid cells were adoptively transferred to corneal recipients by retroorbital injection after corneal transplantation. ... BackgroundAdoptively transferring different sources of myeloid-derived suppressor cells (MDSCs) may assist in mice corneal ...
Consistently, adoptive transfer of Vγ2Vδ2 T cells attenuated TB pathology and contained lesions primarily in the infection site ... Consistently, adoptive transfer of Vγ2Vδ2 T cells attenuated TB pathology and contained lesions primarily in the infection site ... Consistently, adoptive transfer of Vγ2Vδ2 T cells attenuated TB pathology and contained lesions primarily in the infection site ... Consistently, adoptive transfer of Vγ2Vδ2 T cells attenuated TB pathology and contained lesions primarily in the infection site ...
Preliminary studies on artificial immunization against Plasmodium berghei and adoptive transfer of this immunity by splenic T ...
Cells were expanded and sorted by fluorescent marker before adoptive transfer.. Real-time PCR. RNA was isolated via RNeasy Plus ... c, MitoTracker geometric MFI of OT-I T cells transferred as in b. WT n = 7 mice, Blimp-1-deficient n = 8 mice. d, E8ICreERT2 ... Transfer of naive, Blimp-1-deficient OT-I TCR, Tg T cells into mice bearing B16OVA tumors (Extended Data Fig. 5b) showed that ... i, Flow cytometric quantification of OT-I T cells generated as in a, then adoptively transferred into B6 mice infected ...
Adoptive transfer of Chlamydia-pulsed ENO1 knockdown DCs were poorly immunogenic in vitro and in vivo, especially the ability ... T-cell activation and adoptive transfer assays were also used to study DC function. The results showed that ENO1 knockdown DCs ... To ensure quality control and to facilitate transfer of the assay to external laboratories or testing facilities, the in-house ... Transition planning discussions with a provider may increase rates of transfer, yet little is known about frequency and content ...
Moreover, depletion of macrophages attenuated the IL-25/IL-33-induced type 2 immunity in mice, while adoptive transfer of IL-33 ...
Adoptive transfer of retina-specific T cells and LXA4 treatment. Request a detailed protocol To elicit EAU by adoptive T cell ... In the adoptive transfer experiment of T cells, they transfer the T cells from the EAU mice. These T cells are already ... I) Fundoscopy scores of a representative adoptive transfer experiment out of 3. 5 × 106 R161H CD90.1 lymphocytes were activated ... adoptive transfer of either Alox5-/- or Fpr2-/- T cells to TCRb-/- recipients caused exacerbated EAU (Figure 5-figure ...
The adoptive cell transfer procedure (ACT) [43]. There are several types of ACT, but the most popular is (CAR) T-cell therapy ... Adoptive cell therapy (ACT) is a promising approach that involves the intervention of the patients immune system to fight ... adoptive T cell therapy, cancer vaccine, and oncolytic virus. ...
Adoptive cell transfer (ACT) is a form of personalised immunotherapy which has shown promising results in metastasised cancer. ... Adoptive cell transfer (ACT) is a form of personalised immunotherapy which has shown promising results in metastasised cancer. ...
Adoptive T cell transfer. The presence of tumor-reactive T cells has been associated with the success of ICIs (68,69). When ... Rohaan MW, van den Berg JH, Kvistborg P and Haanen JBAG: Adoptive transfer of tumor-infiltrating lymphocytes in melanoma: A ... Foley KC, Nishimura MI and Moore TV: Combination immunotherapies implementing adoptive T-cell transfer for advanced-stage ... The adoptive transfer of autologous T cells transduced with a retrovirus encoding a TCR against an HLA-A*0201 restricted NY-ESO ...
An adoptive transfer method to detect low-dose radiation-induced bystander effects in vivo. Radiat Res. 2010;173(2):125-37. ... Heat shock proteins are transferred between cell types in the nervous system. Thus, stress tolerance in neurons is not solely ... The availability of transgenic animals and gene transfer allowing over-expression of the gene encoding for HSP70, has revealed ... dependent on their own HSP, but can be supplemented by additional HSP transferred from adjacent glial cells. Therefore, ...
A phase II clinical trial of adoptive transfer of haploidentical natural killer cells for consolidation therapy of pediatric ... We use genome-wide CRISPR screens to understand the mechanisms of tumor response to and evasion from adoptive CAR T therapy. ... 1) monoclonal antibody therapy 2) cytokine biology 3) natural killer (NK) cell biology 4) adoptive CAR T therapy 5) mouse ... Altogether, these efforts are geared towards finding new adoptive therapies to treat patients with neuroblastoma. ...
The adoptive transfer of Treg cells prevented hypertension induced by AngII and aldosterone [27]. Implantation of normal thymus ... After electrophoresis, proteins were transferred to a transmembrane, and the membranes were incubated with antibodies against ...
Other Disease Areas and Adoptive Transfer Models. Gfap. (glial fibrillary acidic protein) ... Some Emerging Models require execution of Material Transfer Agreements (MTAs) before orders may be placed. Please check the ...
  • To examine whether tumors in these mice were principally accessible to immunotherapy, splenocytes from immune wild-type mice were adoptively transferred into CEA424-SV40 TAg transgenic mice. (
  • These studies highlighted a major role for CD8 + TILs in antitumor immune responses, supporting the use of tumor-specific CD8 + T cells in adoptive immunotherapy. (
  • Adoptive cell transfer (ACT), in particular, is a type of immunotherapy that strengthens a specific kind of immune cell: T cells. (
  • Recent years' success of cancer immunotherapy including monoclonal antibodies (mAbs), cancer vaccines, adoptive cancer therapy and the immune checkpoint therapy has revolutionized traditional cancer treatment. (
  • However, little is known regarding the role of Fas-mediated cytotoxicity by tumor-specific CD8 + CTL in causing immune evasion of Fas resistant variants during adoptive immunotherapy. (
  • Moreover, TEV were significantly less responsive to eradication by CTL adoptive immunotherapy paradigms as a consequence of increased Fas resistance. (
  • T cell transfer is an investigative immunotherapy in which T cells that target tumors are isolated from patients, manipulated, expanded and then transfused back into those patients. (
  • 2011 Durable and complete response for T cell transfer immunotherapy confirmed for patients with metastatic melanoma. (
  • NK cells play a pivotal role in rapid and highly efficient cancer surveillance and represent a promising avenue for adoptive cell transfer either as a mono- or combination immunotherapy. (
  • The National Cancer Institute (NCI) seeks research co-development partners and/or licensees for a method to identify T cells with preferred phenotypes for increased response from adoptive immunotherapy. (
  • Adoptive T cell immunotherapy is an efficient alternative for dealing with CML individuals, individuals with relapsed CML after HSCT particularly. (
  • Therefore, the ideal technique for adoptive T cell immunotherapy can be to infuse leukemic antigen-specific cytotoxic T lymphocytes (CTLs). (
  • This project holds the promise of increased efficacy and safety of Adoptive Cell Transfer (ACT) as a personalized immunotherapy for cancer patients. (
  • Methods: We adoptively transferred naive Dsg3 -/- splenocytes into Rag2 -/- mice and compared their PV phenoytpe with those mice receiving immunized Dsg3 -/- splenocytes. (
  • 2015). We have adoptively transferred Tregs to evaluate their immunosuppressive function in vivo . (
  • The effect of the adoptively transferred Tregs on tumor growth is then measured by caliper or by in vivo imaging techniques. (
  • 2018). Here, we describe a valuable method to adoptively transfer bone-marrow derived monocytes permitting the assessment of their recruitment and fate in tumors. (
  • These mice were then immunized with the model antigen phycoerythrin (PE), and immune splenocytes (or purified PE-specific B lymphocytes) were adoptively transferred to normal or mutant (EPO-deficient) hosts. (
  • High levels of hEPO were detected in the serum of adoptively transferred normal mice after PE administration, and this responsiveness was maintained for several months. (
  • Myeloid cells were adoptively transferred to corneal recipients by retroorbital injection after corneal transplantation. (
  • A first generation CAR containing a CD4 extracellular domain and a CD3ζ intracellular domain was used in the first clinical trial of chimeric antigen receptor T cells by the biotechnology company Cell Genesys in the mid 1990s, allowing adoptively transferred T cells to target HIV infected cells, although it failed to show any clinical improvement. (
  • To test for functionality, we adoptively transferred TCR-engineered T cells into mice bearing B16 melanomas and observed delayed growth of established tumors with pmel-1 TCR engineered CD8 + T cells and significant tumor regression with TRP-1 TCR transduced CD4 + T cells. (
  • A cloned culture of secondary anti-herpes simplex virus (anti-HSV) cytotoxic T lymphocytes (CTL) generated in vitro when adoptively transferred to intact or cyclophosphamide (CP) pretreated syngeneic mice protected the recipients from death following intraperitoneal infection with HSV-1. (
  • The protective effect of adoptively transferred unstimulated splenocytes shows the presence of ingested interferon α-activated regulatory splenic cell populations that may work via increased interleukin 4 or interleukin 10 production. (
  • While increased CD16 expression was transient in nature, this may not negatively impact the therapeutic potential of engineered NK cells due to the short persistence of adoptively transferred NK cells. (
  • In agreement with the data, beta2TTT/AAA-integrin knock-in DCs brought on a larger T cell response in the spleen than WT DCs (Physique 1F), although as this experiment used small amounts of adoptively transferred DC, which have to survive and migrate in the WT hosts, the CD4 T cell response was not upregulated as much as in Physique 1A, as expected. (
  • Syngeneic lymphocytes were transferred from rodents heavily immunized against the tumor to inhibit growth of small established tumors, becoming the first example of ACT. (
  • In 2002, lymphodepletion using a nonmyeloablative chemotherapy regimen administered immediately before TIL transfer increased cancer regression, as well as the persistent oligoclonal repopulation of the host with the transferred lymphocytes. (
  • Splenic CD4+ T lymphocytes were magnetically separated, incubated with 2.5 {mu}g/ml anti-CD40 ligand ({alpha}CD40L) overnight, and transferred into NP rats on day 12 of gestation (NP+RUPP CD4+ T+anti-CD40L). (
  • In the mid-1980s, Rosenberg and colleagues defined tumor-infiltrating lymphocytes (TILs) as a subset of highly cytotoxic lymphocytes isolated from tumor-bearing patients that exhibited objective responses following adoptive transfer in human cancer patients [ 2 , 3 ]. (
  • The immunogenic nature of melanoma has been useful for the development of adoptive transfer of ex-vivo expanded tumor infiltrating lymphocytes (TIL). (
  • CT056): Durable complete responses to adoptive cell transfer using tumor infiltrating lymphocytes (TIL) in non-small cell lung cancer (NSCLC): a phase I trial. (
  • This report describes an ongoing Phase I clinical trial testing the safety of adoptive cell therapy (ACT) using autologous tumor-infiltrating lymphocytes (TIL) in patients with primary hepatocellular carcinoma (HCC). (
  • The transfer of lymphocytes or ethylene di-isocyanate (HDI), which are used in a serum from sensitized mice can cause clinical disease in naive variety of industries including polyurethane foam mice. (
  • The TCR may be used in an adoptive cell therapy approach utilizing genetically engineered lymphocytes to treat HPV-positive malignancies. (
  • Here, we investigated the specific role of adaptive immunity in this TLE model by adoptive i.v. transfer of immunopurified T cells in mutant mice lacking either CD4(+) T cells (MHCII-knockout), CD8(+) T cells (β2-microglobulin-knockout), or both populations (RAG1-knockout mice). (
  • EEG analysis in mutants mice injected with KA two days after the T cell transfer revealed that grafting of the missing T cell population had no influence on seizure onset, but strongly influenced F4/80(+) macrophage-like cell infiltration in the dentate gyrus. (
  • Specifically, CD8(+) T cells in β2-microgloblin-knockout mice enhanced macrophage recruitment, whereas CD4(+) T cells transferred in MHCII-knockout and in RAG1-knockout mice blocked macrophage infiltration, leading to reduced granule cell dispersion and survival, thereby worsening the KA-induced lesion. (
  • Recently, we developed a PV mouse model by adoptive transfer of splenocytes from recombinant Dsg3-immunized Dsg3 -/- mice to Rag2 -/- immunodeficient mice that expressed Dsg3. (
  • Objectives: We determined whether the adoptive transfer of naive splenocytes from nonimmunized Dsg3 -/- mice induces the anti-Dsg3 IgG production and the PV phenoytpe in recipient mice. (
  • Proteolipid protein peptide 139-151 (PLP 139-151) sensitized spleen cells from female SJL mice were more effective at transferring disease than male cells. (
  • No differences were found for proliferation of transferred TCR-transgenic cells during the early phase of infection, suggesting that T cell priming occurred with the same efficiency in wild-type and ΔDC mice. (
  • Effects of Adoptive Transferring Different Sources of Myeloid-Derived Suppressor Cells in Mice Corneal Transplant Survival. (
  • BackgroundAdoptively transferring different sources of myeloid-derived suppressor cells (MDSCs) may assist in mice corneal transplant survival.MethodsAllogeneic full thickness corneal transplantation (donor C57BL/6 to recipient Balb/c mice) was performed. (
  • However, additional adoptive transfer of MDSCs did not further ameliorate corneal survival in these allogeneic corneal transplantation mice.ConclusionsInflammation-induced MDSC transfer could reduce corneal neovascularization and prolong corneal allograft survival. (
  • Moreover, depletion of macrophages attenuated the IL-25/IL-33-induced type 2 immunity in mice, while adoptive transfer of IL-33-activated macrophages into mice with a chronic Heligmosomoides bakeri infection induced worm expulsion accompanied by a potent type 2 protective immune response. (
  • Adoptive transfer of lung APCs from nCB-exposed wild type to susceptible mice increased tumor incidence and caused early metastasis. (
  • The paper relates how the cancer drug cyclophosphamide (CTX) and OX86 an antibody that activates a molecule named OX40 on T cells were combined with a cutting-edge therapy known as adoptive T cell transfer to eradicate advanced melanoma tumors in mice. (
  • Weizmann Institute scientists have now tested in mice a new form of adoptive cell transfer, which overcomes these limitations while enhancing the tumor-fighting ability of the transferred cells. (
  • If this method works in humans as well as it did in mice, it could lead to an affordable cell transfer therapy for a wide variety of cancers. (
  • Moreover, adoptive transfer of eosinophils significantly reduced liver injury and mortality rate in WT mice. (
  • The extent of bone and cartilage destruction was analysed in human tumour necrosis factor α transgenic (hTNFα tg) mice after adoptive transfer with myeloid specific Atg7-deficient bone marrow. (
  • Transfer experiments into germ-free mice indicated that the HSD-associated microbiota profile is critically dependent on continued exposure to dietary salt. (
  • To investigate the role of the integrin-kindlin-3 conversation in DC-mediated immune responses using MHC class II tetramers following the adoptive transfer of either WT or knock-in LPS-activated peptide-loaded DCs into WT recipient mice. (
  • A number of prospective adoptive parents will relocate interstate at some point during the course of their adoption application. (
  • U.S. Citizenship and Immigration Services Guidance on Determining Suitability of Prospective Adoptive Parents - published on November 9, 2018, this memo discusses UCT as a form of child abuse or neglect. (
  • At one time, prospective adoptive parents may have pursued intercountry adoption in order to avoid dealing with original parents, but today an increasing number of adoptive parents are more informed about the importance of preserving family connections and are creating open adoption relationships with their children's original families abroad, before and after finalization. (
  • Passive immunity is the transfer of active humoral immunity of ready-made antibodies . (
  • Passive immunity is also provided through colostrum and breast milk, which contain IgA antibodies that are transferred to the gut of the infant, providing local protection against disease causing bacteria and viruses until the newborn can synthesize its own antibodies. (
  • Memory T Cells Specific for Murine Cytomegalovirus Re-Emerge after Multiple Challenges and Recapitulate Immunity in Various Adoptive Transfer Scenarios. (
  • The cells taken at these times also contained mediators of antiviral immunity, as determined by a marked reduction of virus titres in the ears of infected animals 1 to 3 days after transfer. (
  • Draining lymph node cells taken at later times contained mediators of virus immunity, but titres were not reduced until day 5 after the transfer. (
  • Transfer of antigen-specific TCR genes into recipient T cells using transgenic method will lead to the transfer of leukemic-specific T cell immunity. (
  • Vγ2Vδ2 T cells for adoptive transfer displayed central/effector memory and mounted effector functions, including the production of anti-M. tuberculosis cytokines and inhibition of intracellular mycobacteria. (
  • My research will focus on the use of ex vivo generation of T regulatory cells for adoptive transfer to patients in different transplant and immunological imbalance settings. (
  • We have previously shown that adoptive transfer of CD4+ T cells from the reduced uterine perfusion pressure (RUPP) rat model of PE increases blood pressure, oxidative stress (ROS), and inflammation in normal pregnant recipient rats. (
  • We have shown that adoptive transfer of haploidentical NK cells after lymphodepleting chemotherapy can induce complete remissions in 30C50% of patients with refractory acute myeloid leukemia (AML) when given with IL-2 to stimulate in vivo donor NK cell growth (2,3). (
  • Previous research has already shown that adoptive transfer of haploidentical NK cells can be effective in reducing disease without the side effects of graft-versus-host disease," said Solving Kids' Cancer Executive Director Scott Kennedy. (
  • Adoptive parents provide a child with a new home and family for life. (
  • Once an adoption order is granted, full parental rights and responsibilities are transferred to the adoptive parents. (
  • We need adoptive parents for single children and to keep brothers and sisters together. (
  • Adoptive parents will be allocated a supervising social worker who will undertake their assessment and provide support, advice and guidance until the adoption process is complete. (
  • HOUSTON ( KIAH ) - The adoptive parents of a young boy who was found dead in a washing machine in July are facing capital murder charges. (
  • Doctors' statements are routinely accepted on faith by the U.S. Embassy, Guatemalan authorities and adoptive American parents. (
  • Adoption - Legal process where parental rights are transferred from birth parents to adoptive parents. (
  • Adoption Assistance - Monthly or one-time only subsidy payments to help adoptive parents raise children with special needs. (
  • Adoption assistance" can also refer to any help given to adoptive parents. (
  • Adopted individuals, birth parents, adoptive parents of minor children, siblings, and others touched by adoption may choose to engage in a search process for a variety of reasons. (
  • National Adoption Information Clearinghouse (NAIC), Access to Family Information by Adopted Persons: Summary of State Laws] Adopted persons age 19 and older and adopted or adoptive parents of minor children may obtain non-identifying medical and background information from an adoption record without an adoption agency violating confidentiality. (
  • A festival of activities and events for families and adoptive parents, to learn, laugh and enjoy. (
  • A Charleston judge on Monday ordered the immediate transfer of 3-year-old Veronica to her adoptive parents and called for action from state and federal authorities after the girl's birth father failed to appear for a court-ordered visit the day before. (
  • The adoptive parents gave the following reason for Karolin's move: "Peer pressure and the need [to] identify and relate to people her own age made our relationship very tense, defying and a battle of willpower. (
  • The adoptive parents and petitioner agree that this arrangement is in the best interests of Karolin, and Karolin wants to live with petitioner. (
  • Petitioner contends that Karolin resides with her and that Karolin's adoptive parents have relinquished custody and control of Karolin to her. (
  • Respondent contends that petitioner has failed to rebut the presumption that Karolin's residence is with her adoptive parents. (
  • 5) Orientation, assessment, and approval of adoptive parents. (
  • Investigations precondition for adoption - contents of investigation report - how conducted - assessments of adoptive parents, contents - waiving of investigation, when - fees - preference to foster parents, when. (
  • The agency and court shall give preference and first consideration for adoptive placements to foster parents. (
  • Similar rules have also been introduced for adoptive parents. (
  • According to the Government Decree No. 1853 of 11/18/2020 (clause 18), Minors under the age of 14 are required to check in the hotel with the documents of their parents (adoptive parents, guardians). (
  • There has been a shift in the kinds of children needing adoptive families, where those children come from, who their original parents are, the kinds of families that adopt them, and the evidence-based principles now considered best practice for adoption policy and practice. (
  • the child's original parents and adoptive parents exchange identifying information and have some form of contact before and after the adoption is finalized. (
  • Decades ago, adoptive parents were generally told, "You must resolve your infertility issues before you can adopt. (
  • How much money do adoptive parents receive? (
  • The adoptive parents of the child eligible to receive title IV-E adoption assistance payments and SSI benefits may make application for both programs and the child, if eligible, may receive benefits from both programs. (
  • Do adoptive parents get paid monthly? (
  • [ 19 ] In addition, impressive clinical responses were observed upon adoptive transfer of tumor-specific autologous T cells using the generation of T cells that expressed cloned T-cell receptors (TCRs) or chimeric antigen receptors (CAR). (
  • Our GMP unit has long-standing experience on the implementation of processing and preparation procedures for adoptive transfer of tumor-reactive T cells and holds official permission for the adoptive transfer of autologous T cells expanded ex vivo. (
  • In this study, we made use of an adoptive transfer model of experimental lung metastasis using tumor-specific CTL as a relevant immune-based selective pressure, and wherein the Fas ligand pathway was involved in the antitumor response. (
  • Adoptive transfer of haploidentical natural killer (NK) cells has shown promise as a treatment option to target and kill cancer cells in a less toxic way than conventional therapies. (
  • En 2018 Sylvain devra subir une deuxième greffe cardiaque après une attente de 4 ans. (
  • This review describes current approaches and their advancement related to monoclonal antibody-related clinical trials, new cytokine therapy, a checkpoint inhibitor, adoptive T cell therapy, cancer vaccine, and oncolytic virus. (
  • In the past decades several clinical trials aimed at eliciting T-cell response with local or systemic immunomodulatory drugs such as interferon (IFN)-α ( 7 , 8 ), interleukin (IL)-2 ( 9 , 10 ), cancer vaccines ( 11 , 12 ) and adoptive cell transfer ( 13 ). (
  • [15] Similar early clinical trials of CAR T cells in solid tumors in the 1990s using first generation CARs targeting a solid tumor antigens such as MUC1 did not show long-term persistence of the transferred T cells or result in significant remissions. (
  • Early studies on the transfer of unmodified autologous or allogeneic NK cells have established their clinical safety and demonstrated modest anti-tumor efficacy. (
  • Only 10% of the human population is homozygous for this polymorphism 8 , suggesting that genetic manipulation of NK cells to express CD16-158V prior to adoptive transfer may improve clinical success not only of NK cell therapies but also anti-tumor IgG1 antibodies. (
  • Non-viral engineering of NK cells using MaxCyte mRNA electroporation provides significant benefits including high efficiency and low toxicity as well as clinical scalability enabling rapid development of novel adoptive cell therapy approaches. (
  • Potentially curative therapies for multiple myeloma (MM) based on various methods of adoptive immune cell transfer are advancing rapidly in clinical trials. (
  • An Associate Professor in the Department of Pathology, Dr. Krogsgaard and her collaborators propose the development of a humanized transgenic mouse model by building artificial human chromosomes that will provide a robust and broadly applicable system for T-cell Receptor (TCR) identification for clinical adoptive cell strategies. (
  • Under this act an employee is entitled to a minimum of 24 consecutive weeks' adoptive leave and an optional 16 weeks' additional adoptive leave. (
  • Should the employee fall ill during the additional adoptive leave, or if he/she has indicated that he/she has the intention to avail of additional adoptive leave and then falls ill, the employee may apply to commence sick leave instead of taking all or the remaining additional adoptive leave. (
  • In vivo adoptive transfer experiments showed that in contrast to naive eosinophils, those pretreated with COX inhibitors failed to attenuate AILI. (
  • The establishment of an in vivo adoptive T-B transfer system could determine for the first time the role of ICOS in T-B cooperation in early immune response stages on antigen specific T and B cell levels. (
  • This is the first comparison of the efficacy of adoptive T cell transfer between transplanted s.c. tumors and autochthonous tumors in the same animals. (
  • am) Transfer guardianship and custody of the child to a county department authorized to accept guardianship under s. 48.57 (1) (hm) for placement of the child for adoption by the child's foster parent, if the county department has agreed to accept guardianship and custody of the child and the foster parent has agreed to adopt the child. (
  • c) Appoint a guardian under s. 48.977 and transfer guardianship and custody of the child to the guardian. (
  • 5) In placing an Indian child in a preadoptive placement following a transfer of guardianship and custody under sub. (
  • Late Wednesday of last week the South Carolina Supreme Court issued a controversial order to the state's family court calling for an expedited transfer of custody to the South Carolina-based adoptive couple without a hearing of best interest for Veronica. (
  • As a result the South Carolina Supreme Court transferred custody to Mr. Brown. (
  • The Uniform Law Commission (ULC) convened a drafting committee to begin development of a draft uniform state law on the unregulated custody transfer (UCT) of adopted children. (
  • After two courts ordered Lisa to transfer custody of her biological daughter to a woman with no adoptive rights, she felt she had no choice but to flee the country. (
  • One very real scenario is that gay adoption will be sought by lesbians who will want the right to obtain or purchase sperm from a sperm bank or an individual man, and then have the state transfer the responsibilities and rights of parenthood from that child's father to an adoptive lesbian … what shall we call her? (
  • The preferred terms, instead, are "the extended family of adoption," "the adoption circle," or "the adoption constellation" in order to capture the realities that adoption joins a child's extended biological and adoptive families in a complex interpersonal network influenced by many societal institutions that play a role in the process. (
  • For example, lifesaving health care information may be unknown to the child's original family at the time of the adoption, so the ongoing communication between the original and adoptive families is essential for the child's emotional and medical well-being. (
  • The monthly adoption assistance payments are determined based upon the child's special needs and the adoptive family's circumstances. (
  • The first section contains the main body of the child health questionnaire along with items transferred from the sample child's person record. (
  • It is standard procedure that adoption proceedings require a hearing to determine the best interest of the child in advance of any transfer proceedings, an essential step the South Carolina Supreme Court failed to take, thus denying Veronica the right to have her best interests considered. (
  • Adoption Attorney - Lawyers who arrange adoptive placements and specialize in adoption. (
  • In transferring from the process and the culture of infertility and its treatment to the process and culture of adoption, consumers are expected to make a huge shift. (
  • 409.1664 Adoption benefits for qualifying adoptive employees of state agencies, veterans, servicemembers, and law enforcement officers. (
  • Applications must be on forms approved by the department and must include a certified copy of the final order of adoption naming the applicant as the adoptive parent. (
  • Adoption requirements: You must be at least 18 to become an adoptive parent. (
  • A case of adoption by a female tufted capuchin who spontaneously adopted a newborn immediately after he was abandoned, then raised him successfully with her own infant, showing no difference in the active care of the adoptive mother. (
  • Adoption assistance (also known as adoption subsidy) provides support that helps adoptive families access medical care, counseling or therapy, special equipment, tutoring programs, and other supports that help them raise their children who have special needs. (
  • 2003). Major techniques used include a wide array of immunological assays, flow cytometry, FACSorting, adoptive in vivo cell transfer and bone marrow transplantation (Binder et al. (
  • Areas covered This review describes the basics of mRNA biotechnology and provides insight into the recent advances in the use of mRNA for the local and systemic delivery of immunostimulatory antibodies, proinflammatory cytokines or for optimizing adoptive T-cell therapy. (
  • Other biotechnology strategies such as IL-12-engineered local adoptive cell therapy and pro-cytokines can also be used to improve results and broaden the therapeutic window. (
  • There was somewhat limited NK PK [pharmacokinetics] in the absence of cytokines, which is a recurring theme, I think, in the allogeneic adoptive-transfer field," he added. (
  • 2010 Study suggests that adoptive transfer of genetically engineered autologous cells can treat patients with b-cell lymphomas. (
  • Using both in vitro coculture assays and a rat model of adoptive transfer after IR, we determined that CDC-conditioned macrophages attenuated cardiomyocyte apoptosis and reduced infarct size, thereby recapitulating the beneficial effects of CDC therapy. (
  • Adoptive transfer of M CDC macrophages reduces infarct size when administered 20 minutes following reperfusion. (
  • We show that adoptive transfer of SL9-specific iPSC-CTLs greatly suppressed EcoHIV replication in the peritoneal macrophages and spleen in the animal model. (
  • This assumption is mainly based on experiments demonstrating that DCs are efficiently infected by LCMV ( 5 ) and efficient T cell priming could be achieved by transfer of LCMV peptide-pulsed DCs ( 6 ), targeting of LCMV peptides for specific uptake by DCs ( 7 ), or selective expression of LCMV peptides in DCs ( 8 ). (
  • In memory recall experiments, performed using OT-I CD8+ T as donor cells, TCXCR4 cells once transferred in vivo maintained higher proportions of CD44Hi CD62LHi CD127+ phenotype cells, underwent higher levels of proliferation and produced higher levels of IFN-γ, early post vaccination with relevant antigen. (
  • Bone marrow adoptive transfers experiments defined the role of hCD39 expression on bone marrow progenitor cells in comparison to tissue expression. (
  • Current immunotherapies involve enhancing the activity of antigen-specific CD8 + TILs through cytokine treatment, immune checkpoint blockade, chimeric antigen receptor therapy, and adoptive T cell transfer (ACT) [ 13 ]. (
  • The adoptive transfer of antigen-specific CD8+ T cells is a promising approach for the treatment of chronic viral and malignant diseases. (
  • We have previously shown that ingested type 1 interferon inhibits chronic relapsing experimental autoimmune encephalomyelitis and the adoptive transfer of experimental autoimmune encephalomyelites by T cells, and decreases both antigen-specific and mitogen-induced pro-inflammatory cytokine secretion in this disorder. (
  • Nevertheless, application of the setting of leukemic antigen-specific T cell adoptive transfer can be often limiting as the isolation and development of leukemic antigen-specific T cells can be labor-intensive and time-consuming [9]. (
  • Therefore, specific TCR gene transfer is an attractive strategy for the fast generation of sufficient numbers of antigen-specific T cells [9]. (
  • Adoptive T cell therapy has shown some promise in treating cancers, but more research is necessary before doctors can use it more widely. (
  • These Guidelines aim to ensure consistent procedures across States and Territories to minimise the disruption to relocating prospective adoptive families. (
  • The "traders" operate like a chain with agents including American host families who provide homes for the pregnant women flown from Israel, doctors, channels for transferring money, and adoptive families who receive the babies one way or another after birth. (
  • CatNap is a private, nonprofit organization dedicated to creating lifelong relationships between companion animals and their adoptive families. (
  • Memory CD8 + T cells (T mem ) are superior mediators of adoptive cell therapy (ACT) compared with effector CD8 + T cells (T eff ) due to increased persistence in vivo . (
  • Adoptive cell transfer therapy has developed into a potent and effective treatment for patients with metastatic melanoma. (
  • Rosenberg, Steven A. / Adoptive Cell Transfer Therapy . (
  • Adoptive T cell therapy, also known as T cell transfer therapy, enhances the body's ability to fight cancer. (
  • In fact, Adoptive cell therapy (ACT) is a promising approach that involves the intervention of the patient's immune system to fight against cancer/tumor cells. (
  • This will enable him to generate chordoma-reactive T cells in the lab and thereby validate these immune targets for adoptive cellular therapy . (
  • Dr. Yee brings together a team of individuals with highly specialized expertise in identification of immune targets, including a computational biologist, an experienced sarcoma clinician, and a T cell laboratory that over the last 20 years has developed a unique platform for validating these immune targets and taking them into the clinic in the form adoptive cell therapy. (
  • We use genome-wide CRISPR screens to understand the mechanisms of tumor response to and evasion from adoptive CAR T therapy. (
  • August 29, 2022 - Notice of Pre-application Webinar for Cancer Adoptive Cellular Therapy Network (Can-ACT) for Adult (RFA-CA-22-028) and Pediatric Cancers (RFA-CA-22-029), and Coordinating Center (RFA-CA-22-030). (
  • Introduction Immune checkpoint inhibitors and adoptive T-cell therapy based on chimeric antigen receptors are the spearhead strategies to exploit the immune system to fight cancer. (
  • In order to improve adoptive T cell therapy (ATT) of cancer, recent strategies aim at the antibody-based blockade of immunosuppressive signaling pathways in CD8+ T cells. (
  • Adoptive T cell therapy is well established in the treatment of haematopoietic cancers, and is under investigation for solid malignancies. (
  • Adoptive transfer of Natural Killer (NK) cells holds promise for a new, non-toxic therapy for OS. (
  • Furthermore, we demonstrate that the adoptive transfer significantly reduced expression of PD-1 on CD4+ T cells in the spleen and generated persistent anti-HIV memory T cells. (
  • They also suppressed the adoptive primary response of spleen cells to FγG in irradiated hosts. (
  • When these cells were transferred together with normal spleen cells into irradiated hosts, suppression of the primary adoptive response to FγG was no longer observed. (
  • Inhibition of an adoptive secondary response to FγG was obtained by transferring tolerant spleen cells with primed B cells provided high doses of tolerant cells were used. (
  • To date, the successful transfer of TCR genes specific for virus-specific and tumor-associated antigens, such as EBV and MART-1 and Wilms' tumor antigen 1 (WT1), has been shown to have specific cytotoxicity for EBV+ lymphoma, leukemia and melanoma [10C13]. (
  • T-cells expressing synthetic chimeric antigen receptors (CARs) have revolutionized immuno-oncology and highlighted the use of adoptive cell transfer, for the treatment of cancer. (
  • Other species besides humans transfer maternal antibodies before birth, including primates and lagomorphs (which includes rabbits and hares). (
  • All other mammalian species predominantly or solely transfer maternal antibodies after birth through milk. (
  • Strikingly, iMDSC transferring significantly reduced neovascularization that was comparable to transferring of tMDSCs, without additional immunosuppression. (
  • Interestingly, the test group of macaques receiving transfer of Vγ2Vδ2 T cells at weeks 1 and 3 after high-dose (500 CFU) M. tuberculosis infection exhibited significantly lower levels of M. tuberculosis infection burdens in lung lobes and extrapulmonary organs than did the control groups receiving PBLs or saline. (
  • Bone marrow transplantation and adoptive transfer approaches demonstrate that ISG15 alters dendritic cell (DC) responses in the ACD mouse model. (
  • Administration of IL-2 after cell transfer enhanced therapeutic potential. (
  • Approaches to target transferred therapeutic T cells to the sites of disease and increase their function and engraftment are desirable. (
  • This initial data suggests that the strategy of inducing CXCR4 over-expression in therapeutic T cells is an attractive approach to enhance malignant disease targeting and favour long-term engraftment of transferred cells. (
  • One of the latest attempts to boost the body's defenses against cancer is called adoptive cell transfer, in which patients receive a therapeutic injection of their own immune cells. (
  • As such, this adoptive transfer could have a great therapeutic impact, in the adjuvant setting, on many different cancer types. (
  • Adoptive transfer of unstimulated splenocytes secreting interleukin 4 and interleukin 10 from fed interferon α donors suppressed spontaneous diabetes mellitus in recipients. (
  • These results indicate that preclinical murine models of adoptive immunotherapies are more practical using γ-retroviral rather than lentiviral vectors. (
  • These Tscm cells, generated using GSK-3beta inhibitors, display enhanced survival and proliferation upon transfer, have multipotent capacity to generate all memory and effector T cell subsets, and show increased anti-tumor activity in a humanized mouse tumor model. (
  • Murine norovirus infection does not alter the CD4 + CD45RB high T cell adoptive transfer mouse model of inflammatory bowel disease. (
  • The frequency of anti-Dsg3 IgG production depended on the number of transferred naive splenocytes. (
  • Karolin came to live with petitioner in respondent's district because she could not get along with her adoptive mother and father. (
  • 4. The assessment of petitioner or petitioners shall be submitted to the petitioner and to the court prior to the scheduled hearing of the adoptive petition. (
  • 2) The disability or disease of a prospective adoptive parent shall not constitute a basis for a determination that the petitioner is unfit or not suitable to be an adoptive parent without a specific showing that there is a causal relationship between the disability or disease and a substantial and significant risk of harm to a child. (
  • The six clinically eligible patients from this trial were then 'enrolled into one cohort of an ongoing phase II pilot trial of adoptive cell transfer. (
  • Adoptive transfer of normal T cells loaded with oncolytic virus into individuals with cancer would be technically easy to implement both to reduce the distribution of metastases and to vaccinate the affected individual in situ against micrometastatic disease. (