AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and Processes
AdipogenesisAdipocytesPPAR gammaCCAAT-Enhancer-Binding Protein-alphaCell DifferentiationCCAAT-Enhancer-Binding Protein-betaAdipose TissueAdipose Tissue, WhiteAdipocytes, WhiteAzo CompoundsAdipocytes, Brown3T3 CellsMesenchymal Stromal CellsReceptors, Cytoplasmic and NuclearThiazolidinedionesGene Expression RegulationObesityOsteogenesisTranscription FactorsCCAAT-Enhancer-Binding ProteinsAnti-Obesity AgentsFatty Acid-Binding ProteinsLipid MetabolismCCAAT-Enhancer-Binding Protein-deltaSubcutaneous FatGene Knockdown TechniquesCells, CulturedMice, ObeseRNA, Small InterferingMice, Inbred C57BLSignal TransductionInsulinRNA, MessengerReverse Transcriptase Polymerase Chain ReactionEndrinFibroblastsLipodystrophy1-Methyl-3-isobutylxanthineWnt ProteinsDexamethasoneOsteoblastsAdiposityGraves OphthalmopathyMice, KnockoutStem CellsLipogenesisAdipose Tissue, BrownRNA InterferenceCell LineStromal CellsOrbitNuclear Receptor Subfamily 1, Group D, Member 1Down-Regulationbeta CateninChromatin ImmunoprecipitationIntercellular Signaling Peptides and ProteinsNIH 3T3 CellsTrialkyltin CompoundsBlotting, Western
Adipogenesis
The differentiation of pre-adipocytes into mature ADIPOCYTES.
Adipocytes
Cells in the body that store FATS, usually in the form of TRIGLYCERIDES. WHITE ADIPOCYTES are the predominant type and found mostly in the abdominal cavity and subcutaneous tissue. BROWN ADIPOCYTES are thermogenic cells that can be found in newborns of some species and hibernating mammals.
PPAR gamma
A nuclear transcription factor. Heterodimerization with RETINOID X RECEPTOR ALPHA is important in regulation of GLUCOSE metabolism and CELL GROWTH PROCESSES. It is a target of THIAZOLIDINEDIONES for control of DIABETES MELLITUS.
CCAAT-Enhancer-Binding Protein-alpha
A CCAAT-enhancer-binding protein found in LIVER; ADIPOSE TISSUE; INTESTINES; LUNG; ADRENAL GLANDS; PLACENTA; OVARY and peripheral blood mononuclear cells (LEUKOCYTES, MONONUCLEAR). Experiments with knock-out mice have demonstrated that CCAAT-enhancer binding protein-alpha is essential for the functioning and differentiation of HEPATOCYTES and ADIPOCYTES.
Cell Differentiation
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
CCAAT-Enhancer-Binding Protein-beta
A CCAAT-enhancer-binding protein found in LIVER; INTESTINES; LUNG and ADIPOSE TISSUE. It is an important mediator of INTERLEUKIN-6 signaling.
Adipose Tissue
Specialized connective tissue composed of fat cells (ADIPOCYTES). It is the site of stored FATS, usually in the form of TRIGLYCERIDES. In mammals, there are two types of adipose tissue, the WHITE FAT and the BROWN FAT. Their relative distributions vary in different species with most adipose tissue being white.
Adipose Tissue, White
Fatty tissue composed of WHITE ADIPOCYTES and generally found directly under the skin (SUBCUTANEOUS FAT) and around the internal organs (ABDOMINAL FAT). It has less vascularization and less coloration than the BROWN FAT. White fat provides heat insulation, mechanical cushion, and source of energy.
Adipocytes, White
Fat cells with light coloration and few MITOCHONDRIA. They contain a scant ring of CYTOPLASM surrounding a single large lipid droplet or vacuole.
Azo Compounds
Adipocytes, Brown
Fat cells with dark coloration due to the densely packed MITOCHONDRIA. They contain numerous small lipid droplets or vacuoles. Their stored lipids can be converted directly to energy as heat by the mitochondria.
3T3 Cells
Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). Lines have been developed using several different strains of mice. Tissues are usually fibroblasts derived from mouse embryos but other types and sources have been developed as well. The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION.
Mesenchymal Stromal Cells
Bone-marrow-derived, non-hematopoietic cells that support HEMATOPOETIC STEM CELLS. They have also been isolated from other organs and tissues such as UMBILICAL CORD BLOOD, umbilical vein subendothelium, and WHARTON JELLY. These cells are considered to be a source of multipotent stem cells because they include subpopulations of mesenchymal stem cells.
Receptors, Cytoplasmic and Nuclear
Intracellular receptors that can be found in the cytoplasm or in the nucleus. They bind to extracellular signaling molecules that migrate through or are transported across the CELL MEMBRANE. Many members of this class of receptors occur in the cytoplasm and are transported to the CELL NUCLEUS upon ligand-binding where they signal via DNA-binding and transcription regulation. Also included in this category are receptors found on INTRACELLULAR MEMBRANES that act via mechanisms similar to CELL SURFACE RECEPTORS.
Thiazolidinediones
THIAZOLES with two keto oxygens. Members are insulin-sensitizing agents which overcome INSULIN RESISTANCE by activation of the peroxisome proliferator activated receptor gamma (PPAR-gamma).
Gene Expression Regulation
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
Obesity
A status with BODY WEIGHT that is grossly above the acceptable or desirable weight, usually due to accumulation of excess FATS in the body. The standards may vary with age, sex, genetic or cultural background. In the BODY MASS INDEX, a BMI greater than 30.0 kg/m2 is considered obese, and a BMI greater than 40.0 kg/m2 is considered morbidly obese (MORBID OBESITY).
Osteogenesis
The process of bone formation. Histogenesis of bone including ossification.
Transcription Factors
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
CCAAT-Enhancer-Binding Proteins
A class of proteins that were originally identified by their ability to bind the DNA sequence CCAAT. The typical CCAAT-enhancer binding protein forms dimers and consists of an activation domain, a DNA-binding basic region, and a leucine-rich dimerization domain (LEUCINE ZIPPERS). CCAAT-BINDING FACTOR is structurally distinct type of CCAAT-enhancer binding protein consisting of a trimer of three different subunits.
Anti-Obesity Agents
Agents that increase energy expenditure and weight loss by neural and chemical regulation. Beta-adrenergic agents and serotoninergic drugs have been experimentally used in patients with non-insulin dependent diabetes mellitus (NIDDM) to treat obesity.
Fatty Acid-Binding Proteins
Intracellular proteins that reversibly bind hydrophobic ligands including: saturated and unsaturated FATTY ACIDS; EICOSANOIDS; and RETINOIDS. They are considered a highly conserved and ubiquitously expressed family of proteins that may play a role in the metabolism of LIPIDS.
Lipid Metabolism
Physiological processes in biosynthesis (anabolism) and degradation (catabolism) of LIPIDS.
CCAAT-Enhancer-Binding Protein-delta
A member of the C-EBP protein family of transcription factors. It plays a key role in G0 PHASE mammary EPITHELIAL CELL growth arrest, and it is involved in transcriptional regulation of INTERLEUKIN 1; INTERLEUKIN 6; and TUMOR NECROSIS FACTOR-ALPHA.
Subcutaneous Fat
Fatty tissue under the SKIN through out the body.
Gene Knockdown Techniques
The artificial induction of GENE SILENCING by the use of RNA INTERFERENCE to reduce the expression of a specific gene. It includes the use of DOUBLE-STRANDED RNA, such as SMALL INTERFERING RNA and RNA containing HAIRPIN LOOP SEQUENCE, and ANTI-SENSE OLIGONUCLEOTIDES.
Cells, Cultured
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Mice, Obese
Mutant mice exhibiting a marked obesity coupled with overeating, hyperglycemia, hyperinsulinemia, marked insulin resistance, and infertility when in a homozygous state. They may be inbred or hybrid.
RNA, Small Interfering
Small double-stranded, non-protein coding RNAs (21-31 nucleotides) involved in GENE SILENCING functions, especially RNA INTERFERENCE (RNAi). Endogenously, siRNAs are generated from dsRNAs (RNA, DOUBLE-STRANDED) by the same ribonuclease, Dicer, that generates miRNAs (MICRORNAS). The perfect match of the siRNAs' antisense strand to their target RNAs mediates RNAi by siRNA-guided RNA cleavage. siRNAs fall into different classes including trans-acting siRNA (tasiRNA), repeat-associated RNA (rasiRNA), small-scan RNA (scnRNA), and Piwi protein-interacting RNA (piRNA) and have different specific gene silencing functions.
Mice, Inbred C57BL
Signal Transduction
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Insulin
A 51-amino acid pancreatic hormone that plays a major role in the regulation of glucose metabolism, directly by suppressing endogenous glucose production (GLYCOGENOLYSIS; GLUCONEOGENESIS) and indirectly by suppressing GLUCAGON secretion and LIPOLYSIS. Native insulin is a globular protein comprised of a zinc-coordinated hexamer. Each insulin monomer containing two chains, A (21 residues) and B (30 residues), linked by two disulfide bonds. Insulin is used as a drug to control insulin-dependent diabetes mellitus (DIABETES MELLITUS, TYPE 1).
RNA, Messenger
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Reverse Transcriptase Polymerase Chain Reaction
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
Endrin
An organochlorine compound that was formerly used as an insecticide. Its manufacture and use has been discontinued in the United States. (From Merck Index, 11th ed)
Fibroblasts
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
Lipodystrophy
A collection of heterogenous conditions resulting from defective LIPID METABOLISM and characterized by ADIPOSE TISSUE atrophy. Often there is redistribution of body fat resulting in peripheral fat wasting and central adiposity. They include generalized, localized, congenital, and acquired lipodystrophy.
1-Methyl-3-isobutylxanthine
A potent cyclic nucleotide phosphodiesterase inhibitor; due to this action, the compound increases cyclic AMP and cyclic GMP in tissue and thereby activates CYCLIC NUCLEOTIDE-REGULATED PROTEIN KINASES
Wnt Proteins
Wnt proteins are a large family of secreted glycoproteins that play essential roles in EMBRYONIC AND FETAL DEVELOPMENT, and tissue maintenance. They bind to FRIZZLED RECEPTORS and act as PARACRINE PROTEIN FACTORS to initiate a variety of SIGNAL TRANSDUCTION PATHWAYS. The canonical Wnt signaling pathway stabilizes the transcriptional coactivator BETA CATENIN.
Dexamethasone
An anti-inflammatory 9-fluoro-glucocorticoid.
Osteoblasts
Bone-forming cells which secrete an EXTRACELLULAR MATRIX. HYDROXYAPATITE crystals are then deposited into the matrix to form bone.
Adiposity
The amount of fat or lipid deposit at a site or an organ in the body, an indicator of body fat status.
Graves Ophthalmopathy
An autoimmune disorder of the EYE, occurring in patients with Graves disease. Subtypes include congestive (inflammation of the orbital connective tissue), myopathic (swelling and dysfunction of the extraocular muscles), and mixed congestive-myopathic ophthalmopathy.
Mice, Knockout
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
Stem Cells
Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.
Lipogenesis
De novo fat synthesis in the body. This includes the synthetic processes of FATTY ACIDS and subsequent TRIGLYCERIDES in the LIVER and the ADIPOSE TISSUE. Lipogenesis is regulated by numerous factors, including nutritional, hormonal, and genetic elements.
Adipose Tissue, Brown
A thermogenic form of adipose tissue composed of BROWN ADIPOCYTES. It is found in newborns of many species including humans, and in hibernating mammals. Brown fat is richly vascularized, innervated, and densely packed with MITOCHONDRIA which can generate heat directly from the stored lipids.
RNA Interference
A gene silencing phenomenon whereby specific dsRNAs (RNA, DOUBLE-STRANDED) trigger the degradation of homologous mRNA (RNA, MESSENGER). The specific dsRNAs are processed into SMALL INTERFERING RNA (siRNA) which serves as a guide for cleavage of the homologous mRNA in the RNA-INDUCED SILENCING COMPLEX. DNA METHYLATION may also be triggered during this process.
Cell Line
Established cell cultures that have the potential to propagate indefinitely.
Stromal Cells
Connective tissue cells of an organ found in the loose connective tissue. These are most often associated with the uterine mucosa and the ovary as well as the hematopoietic system and elsewhere.
Orbit
Bony cavity that holds the eyeball and its associated tissues and appendages.
Nuclear Receptor Subfamily 1, Group D, Member 1
A DNA-binding orphan nuclear receptor that negatively regulates expression of ARNTL TRANSCRIPTION FACTORS and plays a role as a regulatory component of the circadian clock system. The Nr1d1 nuclear receptor expression is cyclically-regulated by a feedback loop involving its positive regulation by CLOCK PROTEIN; BMAL1 PROTEIN heterodimers and its negative regulation by CRYPTOCHROME and PERIOD PROTEINS.
Down-Regulation
A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
beta Catenin
A multi-functional catenin that participates in CELL ADHESION and nuclear signaling. Beta catenin binds CADHERINS and helps link their cytoplasmic tails to the ACTIN in the CYTOSKELETON via ALPHA CATENIN. It also serves as a transcriptional co-activator and downstream component of WNT PROTEIN-mediated SIGNAL TRANSDUCTION PATHWAYS.
Chromatin Immunoprecipitation
A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of CHROMATIN to crosslink the DNA-BINDING PROTEINS to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific ANTIBODIES. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing.
Intercellular Signaling Peptides and Proteins
Regulatory proteins and peptides that are signaling molecules involved in the process of PARACRINE COMMUNICATION. They are generally considered factors that are expressed by one cell and are responded to by receptors on another nearby cell. They are distinguished from HORMONES in that their actions are local rather than distal.
NIH 3T3 Cells
A continuous cell line of high contact-inhibition established from NIH Swiss mouse embryo cultures. The cells are useful for DNA transfection and transformation studies. (From ATCC [Internet]. Virginia: American Type Culture Collection; c2002 [cited 2002 Sept 26]. Available from http://www.atcc.org/)
Trialkyltin Compounds
Organometallic compounds which contain tin and three alkyl groups.
Blotting, Western
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.

Induction of chondro-, osteo- and adipogenesis in embryonic stem cells by bone morphogenetic protein-2: effect of cofactors on differentiating lineages. (1/1177)

BACKGROUND: Recently, tissue engineering has merged with stem cell technology with interest to develop new sources of transplantable material for injury or disease treatment. Eminently interesting, are bone and joint injuries/disorders because of the low self-regenerating capacity of the matrix secreting cells, particularly chondrocytes. ES cells have the unlimited capacity to self-renew and maintain their pluripotency in culture. Upon induction of various signals they will then differentiate into distinctive cell types such as neurons, cardiomyocytes and osteoblasts. RESULTS: We present here that BMP-2 can drive ES cells to the cartilage, osteoblast or adipogenic fate depending on supplementary co-factors. TGFbeta1, insulin and ascorbic acid were identified as signals that together with BMP-2 induce a chondrocytic phenotype that is characterized by increased expression of cartilage marker genes in a timely co-ordinated fashion. Expression of collagen type IIB and aggrecan, indicative of a fully mature state, continuously ascend until reaching a peak at day 32 of culture to approximately 80-fold over control values. Sox9 and scleraxis, cartilage specific transcription factors, are highly expressed at very early stages and show decreased expression over the time course of EB differentiation. Some smaller proteoglycans, such as decorin and biglycan, are expressed at earlier stages. Overall, proteoglycan biosynthesis is up-regulated 7-fold in response to the supplements added. BMP-2 induced chondrocytes undergo hypertrophy and begin to alter their expression profile towards osteoblasts. Supplying mineralization factors such as beta-glycerophosphate and vitamin D3 with the culture medium can facilitate this process. Moreover, gene expression studies show that adipocytes can also differentiate from BMP-2 treated ES cells. CONCLUSIONS: Ultimately, we have found that ES cells can be successfully triggered to differentiate into chondrocyte-like cells, which can further alter their fate to become hypertrophic, and adipocytes. Compared with previous reports using a brief BMP-2 supplementation early in differentiation, prolonged exposure increased chondrogenic output, while supplementation with insulin and ascorbic acid prevented dedifferentiation. These results provide a foundation for the use of ES cells as a potential therapy in joint injury and disease.  (+info)

Role of Gas-6 in adipogenesis and nutritionally induced adipose tissue development in mice. (2/1177)

OBJECTIVE: A potential role of growth arrest-specific gene 6 (Gas-6) in energy storage in adipose tissue was investigated in murine models of obesity. Gas-6 is a ligand for the Axl, C-Mer, and Sky family of tyrosine kinase receptors. METHODS AND RESULTS: Whereas Gas-6, C-Mer, and Sky were expressed in mature murine adipocytes, the expression of Axl was restricted to the stromal-vascular fraction, which includes pre-adipocytes. During the in vitro conversion of adipogenic 3T3-F442A cells into mature adipocytes, the expression of Gas-6 increased in undifferentiated confluent pre-adipocytes during a transient phase of growth arrest. On treatment of these cells with an adipogenic medium, Gas-6 expression decreased sharply, coinciding with expression of early adipocytes markers. This modulation was not observed in the nonadipogenic 3T3-C2 cells. The Gas-6 mRNA level was transiently downregulated during nutritionally induced expansion of adipose tissues in vivo. When kept on a standard diet, no significant difference in either total body weight or weight of gonadal or subcutaneous fat pads was observed between Gas-6 deficient and wild-type mice. On exposure to a high-fat diet, however, Gas-6-deficient mice had significantly less fat mass than their wild-type counterparts. CONCLUSIONS: Gas-6 enhances the accumulation of adipose tissue in diet-induced obese mice.  (+info)

Mesenchymal stem cells from the outer ear: a novel adult stem cell model system for the study of adipogenesis. (3/1177)

Adipocytes arise from multipotent stem cells of mesodermal origin, which also give rise to the muscle, bone, and cartilage lineages. However, signals and early molecular events that commit multipotent stem cells into the adipocyte lineage are not well established mainly due to lack of an adequate model system. We have identified a novel source of adult stem cells from the external murine ears referred to here as an ear mesenchymal stem cells (EMSC). EMSC have been isolated from several standard and mutant strains of mice. They are self-renewing, clonogenic, and multipotent, since they give rise to osteocytes, chondrocytes, and adipocytes. The in vitro characterization of EMSC indicates very facile adipogenic differentiation. Morphological, histochemical, and molecular analysis after the induction of differentiation showed that EMSC maintain adipogenic potentials up to fifth passage. A comparison of EMSC to the stromal-vascular (S-V) fraction of fat depots, under identical culture conditions (isobutyl-methylxanthine, dexamethasone, and insulin), revealed much more robust and consistent adipogenesis in EMSC than in the S-V fraction. In summary, we show that EMSC can provide a novel, easily obtainable, primary culture model for the study of adipogenesis.  (+info)

Generation of a vascularized organoid using skeletal muscle as the inductive source. (4/1177)

The technology required for creating an in vivo microenvironment and a neovasculature that can grow with and service new tissue is lacking, precluding the possibility of engineering complex three-dimensional organs. We have shown that when an arterio-venous (AV) loop is constructed in vivo in the rat groin, and placed inside a semisealed chamber, an extensive functional vasculature is generated. To test whether this unusually angiogenic environment supports the survival and growth of implanted tissue or cells, we inserted various preparations of rat and human skeletal muscle. We show that after 6 weeks incubation of muscle tissue, the chamber filled with predominantly well-vascularized recipient-derived adipose tissue, but some new donor-derived skeletal muscle and connective tissue were also evident. When primary cultured myoblasts were inserted into the chamber with the AV loop, they converted to mature striated muscle fibers. Furthermore, we identify novel adipogenesis-inducing properties of skeletal muscle. This represents the first report of a specific three-dimensional tissue grown on its own vascular supply.  (+info)

The transcription factor GATA2 regulates differentiation of brown adipocytes. (5/1177)

Brown adipose tissue (BAT) is a specialized mammalian tissue and a site of adaptive thermogenesis. Although the metabolic functions of brown and white adipocytes are distinct, terminal differentiation of both adipocyte lineages is regulated by well-characterized common transcription factors. However, the early stages of adipocyte differentiation and regulation of precursor cells are not well understood. We report here that GATA2 is expressed in brown adipocyte precursors, and its expression is downregulated in a differentiation-dependent manner. Constitutive expression of GATA2 suppressed expression of BAT-specific genes in brown adipocytes, whereas disruption of a GATA2 allele in brown preadipocytes resulted in significantly elevated differentiation and expression of several markers of brown adipogenesis. Collectively, these results show that GATA2 functions to suppress brown adipocyte differentiation, whereas reduction of GATA2 promotes brown adipogenesis.  (+info)

The G0/G1 switch gene 2 is a novel PPAR target gene. (6/1177)

PPARs (peroxisome-proliferator-activated receptors) alpha, beta/delta and gamma are a group of transcription factors that are involved in numerous processes, including lipid metabolism and adipogenesis. By comparing liver mRNAs of wild-type and PPARalpha-null mice using microarrays, a novel putative target gene of PPARalpha, G0S2 (G0/G1 switch gene 2), was identified. Hepatic expression of G0S2 was up-regulated by fasting and by the PPARalpha agonist Wy14643 in a PPARalpha-dependent manner. Surprisingly, the G0S2 mRNA level was highest in brown and white adipose tissue and was greatly up-regulated during mouse 3T3-L1 and human SGBS (Simpson-Golabi-Behmel syndrome) adipogenesis. Transactivation, gel shift and chromatin immunoprecipitation assays indicated that G0S2 is a direct PPARgamma and probable PPARalpha target gene with a functional PPRE (PPAR-responsive element) in its promoter. Up-regulation of G0S2 mRNA seemed to be specific for adipogenesis, and was not observed during osteogenesis or myogenesis. In 3T3-L1 fibroblasts, expression of G0S2 was associated with growth arrest, which is required for 3T3-L1 adipogenesis. Together, these data indicate that G0S2 is a novel target gene of PPARs that may be involved in adipocyte differentiation.  (+info)

Brain and muscle Arnt-like protein-1 (BMAL1), a component of the molecular clock, regulates adipogenesis. (7/1177)

Brain and muscle Arnt-like protein-1 (BMAL1; also known as MOP3 or Arnt3) is a transcription factor known to regulate circadian rhythm. Here, we established its involvement in the control of adipogenesis and lipid metabolism activity in mature adipocytes. During adipose differentiation in 3T3-L1 cells, the level of BMAL1 mRNA began to increase 4 days after induction and was highly expressed in differentiated cells. In white adipose tissues isolated from C57BL/6J mice, BMAL1 was predominantly expressed in a fraction containing adipocytes, as compared with the stromal-vascular fraction. BMAL1 knockout mice embryonic fibroblast cells failed to be differentiated into adipocytes. Importantly, adding BMAL1 back by adenovirus gene transfer restored the ability of BMAL1 knockout mice embryonic fibroblast cells to differentiate. Knock-down of BMAL1 expression in 3T3-L1 cells by an RNA interference technique allowed the cells to accumulate only minimum amounts of lipid droplets in the cells. Adenovirus-mediated expression of BMAL1 in 3T3-L1 adipocytes resulted in induction of several factors involved in lipogenesis. The promoter activity of these genes was stimulated in a BMAL1-dependent manner. Interestingly, expression of these factors showed clear circadian rhythm in mice adipose tissue. Furthermore, overexpression of BMAL1 in adipocytes increased lipid synthesis activity. These results indicate that BMAL1, a master regulator of circadian rhythm, also plays important roles in the regulation of adipose differentiation and lipogenesis in mature adipocytes.  (+info)

Gene expression analysis suggests that EBF-1 and PPARgamma2 induce adipogenesis of NIH-3T3 cells with similar efficiency and kinetics. (8/1177)

Differentiation of multipotent mesenchymal stem cells into lipid-accumulating adipocytes is a physiological process induced by transcription factors in combination with hormonal stimulation. We have used Affymetrix microarrays to compare the adipogenic differentiation pathways of NIH-3T3 fibroblasts induced to undergo in vitro differentiation by ectopic expression of early B cell factor (EBF)-1 or peroxisome proliferator-activated receptor (PPAR)gamma2. These experiments revealed that commitment to the adipogenic pathway in the NIH-3T3 cells was not reflected in gene expression until 4 days after induction of differentiation. Furthermore, gene expression patterns at the earlier time points after stimulation indicated that EBF-1 and PPARgamma2 induced different sets of genes, while the similarities increased upon differentiation, and that several genes linked to adipocyte differentiation were also transiently induced in the vector-transduced cells. These data suggest that the initial activation of genes associated with adipocyte development is independent of commitment to the adipogenic pathway and that EBF-1 and PPARgamma2 induce adipocyte differentiation with comparable kinetics and efficiency.  (+info)

Comprehensive transcriptome analysis of mouse embryonic stem cell adipogenesis unravels new processes of adipocyte development ...Comprehensive transcriptome analysis of mouse embryonic stem cell adipogenesis unravels new processes of adipocyte development ...
The current epidemic of obesity has caused a surge of interest in the study of adipose tissue formation. While major progress has been made in defining the molecular networks that control adipocyte terminal differentiation, the early steps of adipocyte development and the embryonic origin of this lineage remain largely unknown. Here we performed genome-wide analysis of gene expression during adipogenesis of mouse embryonic stem cells (ESCs). We then pursued comprehensive bioinformatic analyses, including de novo functional annotation and curation of the generated data within the context of biological pathways, to uncover novel biological functions associated with the early steps of adipocyte development. By combining in-depth gene regulation studies and in silico analysis of transcription factor binding site enrichment, we also provide insights into the transcriptional networks that might govern these early steps. This study supports several biological findings: firstly, adipocyte development in mouse
https://genomebiology.biomedcentral.com/articles/10.1186/gb-2010-11-8-r80
PLOS ONE: Zfp423 Promotes Adipogenic Differentiation of Bovine Stromal Vascular CellsPLOS ONE: Zfp423 Promotes Adipogenic Differentiation of Bovine Stromal Vascular Cells
Intramuscular fat or marbling is critical for the palatability of beef. In mice, very recent studies show that adipocytes and fibroblasts share a common pool of progenitor cells, with Zinc finger protein 423 (Zfp423) as a key initiator of adipogenic differentiation. To evaluate the role of Zfp423 in intramuscular adipogenesis and marbling in beef cattle, we sampled beef muscle for separation of stromal vascular cells. These cells were immortalized with pCI neo-hEST2 and individual clones were selected by G418. A total of 288 clones (3×96 well plates) were isolated and induced to adipogenesis. The presence of adipocytes was assessed by Oil-Red-O staining. Three clones with high and low adipogenic potential respectively were selected for further analyses. In addition, fibro/adipogenic progenitor cells were selected using a surface marker, platelet derived growth factor receptor (PDGFR) α. The expression of Zfp423 was much higher (307.4±61.9%, P|0.05) in high adipogenic cells, while transforming growth
http://journals.plos.org/plosone/article/comments?id=10.1371/journal.pone.0047496&imageURI=info:doi/10.1371/journal.pone.0047496.g001
COL18A1 is highly expressed during human adipocyte differentiation and the SNP c.1136C | T in its frizzled motif is...COL18A1 is highly expressed during human adipocyte differentiation and the SNP c.1136C | T in its frizzled motif is...
ERRERA, Flavia I.V. et al. COL18A1 is highly expressed during human adipocyte differentiation and the SNP c.1136C , T in its frizzled motif is associated with obesity in diabetes type 2 patients. An. Acad. Bras. Ciênc. [online]. 2008, vol.80, n.1, pp.167-177. ISSN 1678-2690. http://dx.doi.org/10.1590/S0001-37652008000100012.. Collagen XVIII can generate two fragments, NC11-728 containing a frizzled motif which possibly acts in Wnt signaling and Endostatin, which is cleaved from the NC1 and is a potent inhibitor of angiogenesis. Collagen XVIII and Wnt signaling have recently been associated with adipogenic differentiation and obesity in some animal models, but not in humans. In the present report, we have shown that COL18A1 expression increases during human adipogenic differentiation. We also tested if polymorphisms in the Frizzled (c.1136C,T; Thr379Met) and Endostatin (c.4349G,A; Asp1437Asn) regions contribute towards susceptibility to obesity in patients with type 2 diabetes (113 obese, BMI ...
https://www.scielo.br/scielo.php?script=sci_abstract&pid=S0001-37652008000100012&lng=pt&nrm=iso&tlng=en
Adipogenesis in Obesity Requires Close Interplay Between Differentiating Adipocytes, Stromal Cells, and Blood Vessels | DiabetesAdipogenesis in Obesity Requires Close Interplay Between Differentiating Adipocytes, Stromal Cells, and Blood Vessels | Diabetes
Despite detailed knowledge about the molecular mechanisms that control adipocyte differentiation in vitro, little is known about how adipogenesis progresses in vivo, particularly in obesity. Our live-cell imaging has revealed that adipogenesis takes place within adipogenic/angiogenic cell clusters that also contain various stromal cells and blood vessels and that angiogenesis is an essential part of adipogenesis in obesity.. Macrophages reportedly accumulate within obese adipose tissue (11,28) so that the presence of macrophage markers is significantly and positively correlated with both adipocyte size and body mass (34). However, little is known about the roles such macrophages play in adipogenesis and obesity. Although infiltration by macrophages of the area surrounding small adipocytes has been previously described in older obese animals (11,28), their functions have been related to adipocyte cell death (22). The present report is the first, to our knowledge, to describe adipocyte-associated ...
https://diabetes.diabetesjournals.org/content/56/6/1517.full
Dynamics of mRNA and polysomal abundance in early 3T3-L1 adipogenesis | BMC Genomics | Full TextDynamics of mRNA and polysomal abundance in early 3T3-L1 adipogenesis | BMC Genomics | Full Text
Adipogenesis is a complex process, in which immature pre-adipocytes change morphology, micro-anatomy and physiology to become mature adipocytes. These store and accumulate fat and release diverse hormones. Massive changes in protein content and protein composition of the transforming cell take place within a short time-frame. In a previous study we analyzed changes in the abundance of free and polysomal, i.e. ribosome bound, RNAs in the first hours of adipogenesis in the murine cell line 3T3-L1. Here we analyze changes of mRNA levels and their potential contribution to the changing protein pool by determination of mRNA levels and ribosome binding to mRNAs in 3T3-L1 cells stimulated for adipogenesis. We grouped mRNA species into categories with respect to up- or down-regulated transcription and translation and analyzed the groups regarding specific functionalities based on Gene Ontology (GO). A shift towards up-regulation of gene expression in early adipogenesis was detected. Genes up-regulated at the
https://0-bmcgenomics-biomedcentral-com.brum.beds.ac.uk/articles/10.1186/1471-2164-15-381
Louisiana Tech Digital Commons - Undergraduate Research Symposium: 06. The Influence of MED12 Knockdown on AdipogenesisLouisiana Tech Digital Commons - Undergraduate Research Symposium: 06. The Influence of MED12 Knockdown on Adipogenesis
The ubiquity of obesity has increased exponentially, and the health burden of obesity-related diseases including type 2 diabetes, metabolic disorders, heart diseases, and some types of cancers is growing. Obesity is characterized by the excess accumulation of fat and adipose tissue and driven by adipogenesis, which is the process in which stem cells differentiate into adipocytes. We utilize human adipose derived stem cells (hASCs) isolated from adult fat tissue to study adipogenesis (the formation of fat tissue). This physiological potential, combined with non-invasive collection methods, make hASCs favorable in the search for new clinical stem cell treatments and for the study of cellular processes and differentiation. We are interested in understanding the function of MED12 in adipogenesis and determining its role in initiating cell type specific gene expression in hopes that this research can be used in treatments for obesity and related metabolic disorders. MED12 is a subunit of the Mediator complex
https://digitalcommons.latech.edu/undergraduate-research-symposium/2021/poster-presentations/4/
Ursolic Acid Inhibits Adipogenesis in 3T3-L1 Adipocytes through LKB1/AMPK PathwayUrsolic Acid Inhibits Adipogenesis in 3T3-L1 Adipocytes through LKB1/AMPK Pathway
Background Ursolic acid (UA) is a triterpenoid compound with multiple biological functions. This compound has recently been reported to possess an anti-obesity effect; however, the mechanisms are less understood. Objective As adipogenesis plays a critical role in obesity, the present study was conducted to investigate the effect of UA on adipogenesis and mechanisms of action in 3T3-L1 preadipocytes. Methods and Results The 3T3-L1 preadipocytes were induced to differentiate in the presence or absence of UA for 6 days. The cells were determined for proliferation, differentiation, fat accumulation as well as the protein expressions of molecular targets that regulate or are involved in fatty acid synthesis and oxidation. The results demonstrated that ursolic acid at concentrations ranging from 2.5 µM to 10 µM dose-dependently attenuated adipogenesis, accompanied by reduced protein expression of CCAAT element binding protein β (C/EBPβ), peroxisome proliferator-activated receptor γ (PPARγ), CCAAT
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0070135
Regulation of brown fat adipogenesis by protein tyrosine phosphatase 1B<...
TY - JOUR. T1 - Regulation of brown fat adipogenesis by protein tyrosine phosphatase 1B. AU - Matsuo, Kosuke. AU - Bettaieb, Ahmed. AU - Nagata, Naoto. AU - Matsuo, Izumi. AU - Keilhack, Heike. AU - Haj, Fawaz. PY - 2011. Y1 - 2011. N2 - Background: Protein-tyrosine phosphatase 1B (PTP1B) is a physiological regulator of insulin signaling and energy balance, but its role in brown fat adipogenesis requires additional investigation. Methodology/Principal Findings: To precisely determine the role of PTP1B in adipogenesis, we established preadipocyte cell lines from wild type and PTP1B knockout (KO) mice. In addition, we reconstituted KO cells with wild type, substratetrapping (D/A) and sumoylation-resistant (K/R) PTP1B mutants, then characterized differentiation and signaling in these cells. KO, D/A- and WT-reconstituted cells fully differentiated into mature adipocytes with KO and D/A cells exhibiting a trend for enhanced differentiation. In contrast, K/R cells exhibited marked attenuation in ...
https://ucdavis.pure.elsevier.com/en/publications/regulation-of-brown-fat-adipogenesis-by-protein-tyrosine-phosphat
PSRC - Platelet-rich Plasma Promotes Fat Graft Survival via Stemness and Angiogenesis on Adipose-derived Stem CellsPSRC - Platelet-rich Plasma Promotes Fat Graft Survival via Stemness and Angiogenesis on Adipose-derived Stem Cells
Human Platelets were purchased from HEMOCARE for the production of PRP. Human adipose-derived stem cells were isolated as per laboratory protocol. The ASCs were cultured under four conditions: 1. Regular DMEM medium, 2. Regular DMEM medium with PRP 3. Adipogenic medium 4. Adipogenic medium with PRP. The cell proliferation was assessed by CYQUANT and the adipogenesis were evaluated by AdipoRed stain and the qPCR of PPAR-gamma and FABP4 gene expression. The mRNA of stemness gene expression of ASCs was compared by qPCR of SOX-2, Nanog and Oct-4 .The angiogenesis of ASCs was evaluated by qPCR of VEGF gene expression and endothelium tube formation assay. The nude mice were implant with fat graft with PRP as experiment and fat graft only as control group. The survival rate was analyzed by volume retention, the histomophometry of mature adipocyte area and vessel density assay by CD31 immunohistochemical stain ...
http://ps-rc.org/meeting/abstracts/2014/130.cgi
Akt‐ and Foxo1‐interacting WD‐repeat‐FYVE protein promotes adipogenesis | The EMBO Journal
We have previously identified a WD‐repeat propeller‐FYVE protein, ProF, as an interaction partner for the kinases PKCζ and Akt in 3T3‐L1 cells (Fritzius et al, 2006). Furthermore, we have shown that ProF forms a trimeric complex with PKCζ and its substrate VAMP2. In this complex, ProF was found to act as an adaptor‐like protein for facilitated substrate phosphorylation of VAMP2 by the active kinase PKCζ (Fritzius et al, 2007).. In this study, we identified a role for ProF during adipogenesis and showed complex formation of ProF with the kinase Akt and the kinase substrate Foxo1. The protein kinase Akt was found to affect adipogenesis after the expression of C/EBPβ and C/EBPδ, but before the expression of PPARγ and C/EBPα (Peng et al, 2003; Baudry et al, 2006), which resembled the effects of ProF on adipogenesis. Akt phosphorylation of Foxo1 at Ser253, in the Foxo1 DNA binding domain, has been demonstrated to decrease its transcriptional activity (Zhang et al, 2002). We have shown ...
http://emboj.embopress.org/content/27/9/1399
CXCL3 positively regulates adipogenic differentiation<...
TY - JOUR. T1 - CXCL3 positively regulates adipogenic differentiation. AU - Kusuyama, Joji. AU - Komorizono, Anna. AU - Bandow, Kenjiro. AU - Ohnishi, Tomokazu. AU - Matsuguchi, Tetsuya. PY - 2016/10. Y1 - 2016/10. N2 - Chemokines are a family of cytokines inducing cell migration and inflammation. Recent reports have implicated the roles of chemokines in cell differentiation. However, little is known about the functional roles of chemokines in adipocytes. Here, we explored gene expression levels of chemokines and chemokine receptors during adipogenic differentiation. We have found that two chemokines, chemokine (C-X-C motif) ligand 3 (CXCL3) and CXCL13, as well as CXC chemokine receptor 2(CXCR2), a CXCL3 receptor, are highly expressed in mature adipocytes. When 3T3-L1 cells and ST2 cells were induced to differentiate, both the number of lipid droplets and the expression levels of adipogenic markers were significantly promoted by the addition of CXCL3, but not CXCL13. Conversely, gene knockdown ...
https://tohoku.pure.elsevier.com/ja/publications/cxcl3-positively-regulates-adipogenic-differentiation
PSRC - REGULATION OF WNT SIGNALING IN ADIPOSE DERIVED STEM CELLS IMPROVES ADIPOGENIC POTENTIALPSRC - REGULATION OF WNT SIGNALING IN ADIPOSE DERIVED STEM CELLS IMPROVES ADIPOGENIC POTENTIAL
University of Pittsburgh Introduction: Human adipose derived stem cells (ASC) may have broad applications to plastic and reconstructive surgery. For soft tissue reconstruction, the ability to induce adipogenesis and angiogenesis is vital for long term graft survival. However the gene expression and cellular fate of these cells is governed by molecular signaling. Wnt signaling is well evidenced in inhibiting adipogenesis and influence cell differentiation. We hypothesized that regulation of this signaling cascade in adipose stem cells could enhance adipogenesis.. Purpose: This study aims to antagonize Wnt signaling by lentiviral overexpression of secreted frizzled-related protein1 (sFRP1) in ASCs to assess adipogenesis and angiogenic growth factor secretion.. Methods: Wnt antagonistic studies were carried out in lentiviral sFRP1 transfected and flow cytometry selected GFP reporter positive ASCs. mRNA gene expression of signaling cascade were analyzed for adipogenic marker genes (PPARy, FABP4, ...
http://www.ps-rc.org/meeting/abstracts/2013/169.cgi
Increased lipid accumulation and adipogenic gene expression of adipocytes in 3D bioprinted nanocellulose scaffoldsIncreased lipid accumulation and adipogenic gene expression of adipocytes in 3D bioprinted nanocellulose scaffolds
Compared to standard 2D culture systems, new methods for 3D cell culture of adipocytes could provide more physiologically accurate data and a deeper understanding of metabolic diseases such as diabetes. By resuspending living cells in a bioink of nanocellulose and hyaluronic acid, we were able to print 3D scaffolds with uniform cell distribution. After one week in culture, cell viability was 95%, and after two weeks the cells displayed a more mature phenotype with larger lipid droplets than standard 2D cultured cells. Unlike cells in 2D culture, the 3D bioprinted cells did not detach upon lipid accumulation. After two weeks, the gene expression of the adipogenic marker genes PPAR. and FABP4 was increased 2.0- and 2.2-fold, respectively, for cells in 3D bioprinted constructs compared with 2D cultured cells. Our 3D bioprinted culture system produces better adipogenic differentiation of mesenchymal stem cells and a more mature cell phenotype than conventional
https://research.chalmers.se/publication/249137
The role of E2F4 in adipogenesis is independent of its cell cycle regulatory activity | PNASThe role of E2F4 in adipogenesis is independent of its cell cycle regulatory activity | PNAS
In this study, we have continued to investigate the roles of the E2F and pocket proteins in the regulation of adipocyte differentiation. It was previously shown that hormone-induced adipogenesis is promoted by the loss of either E2F4 or p107 and p130 (25, 26). It seemed highly likely that the shared activity of E2F4 and p107/p130 simply reflects their participation in transcriptionally repressive complexes. However, our current analyses of compound mutant MEFs do not support this hypothesis. Instead, they suggest that the E2F and pocket proteins contribute to the regulation of adipocyte differentiation through three distinct mechanisms. Moreover, each one of these can be separated from effects on cell cycle control.. The first mechanism involves the E2F4 transcription factor. We have found that E2F4 loss predisposes MEFs to undergo adipogenesis. This phenotype includes increasing the proportion of cells that differentiate in response to the standard hormone treatment as well as enabling ...
https://www.pnas.org/content/100/5/2456
Comparison of maternal isocaloric high carbohydrate and high fat diets on osteogenic and adipogenic genes expression in...Comparison of maternal isocaloric high carbohydrate and high fat diets on osteogenic and adipogenic genes expression in...
To our knowledge, these are the first results that demonstrate the effects of maternal isocaloric pair-fed high-carbohydrate (LF-HCD) versus high-fat diet (HF-LCD) during gestation and lactation on gene expression and serum levels of formation and resorption markers in bone, as well as adipogenic and lipogenic markers in retroperitoneal fat mass of mice offspring at adolescence. The results of the present study showed that maternal LF-HCD during gestation and lactation lead to up-regulation of Runx2 and Ctnnb1, as well as Runx2, OPG, OPG/RANK-L ratio and Ctnnb1 mRNA expression in bone of female and male offspring, respectively. Also, serum levels of OPG/RNK-L ratio which is the marker of osteogenesis [22] were increased in the LF-HCD-fed group, compared with the HF-LCD. PPARγ2 mRNA expression, as well as other adipogenic genes measured in the current study and serum levels of proteins were increased in the offspring of HF-LCD-fed mothers. Our results showed that mRNA expression of OPG and ...
https://nutritionandmetabolism.biomedcentral.com/articles/10.1186/s12986-016-0130-x
The transforming growth factor-beta/bone morphogenetic protein signalling pathway in adipogenesisThe transforming growth factor-beta/bone morphogenetic protein signalling pathway in adipogenesis
Rising obesity epidemic makes the better understanding of transcription factor networks regulating adipogenesis very challenging. Adipogenesis begins with the commitment of pluripotent mesenchymal stem cells to the adipocyte lineage, followed by terminal differentiation of preadipocytes to mature ad …
https://pubmed.ncbi.nlm.nih.gov/22226816/
Analysis of mitochondria morphology dynamics during adipogenesis, UNCG NC DOCKS (North Carolina Digital Online Collection of...
Abstract: There is great concern with an increase in the number of Americans who are overweight and obese. Fat cells or adipocytes play a central role in obesity. These cells are metabolically active and play a fundamental role in energy allocation and storage. The adipocyte functions as the energy storage cell by storing excess energy in the forms of triglycerides in lipid vesicles within the cell. The morphology of mitochondria is a dynamic process that varies from cell type to cell type and in response to a variety of signals and conditions (Wilson-Fritch, 2002; Wilson-Fritch, 2004). The morphology of mitochondria in the cell often reflects the functions of that type of cell. In my thesis I characterize the changes in mitochondrial morphology and actin during adipogenesis. In this thesis I found that mitochondria undergo a radical change in morphology during the first two days of adipogenesis. In the pre-adipocyte cell mitochondria assume a reticular morphology that is distributed uniformly ...
http://libres.uncg.edu/ir/uncg/listing.aspx?styp=ti&id=1185
Plus itPlus it
CUL4B participates in the regulation of a broad spectrum of biological processes. In the current study, we provided several lines of evidence that CUL4B functions as a negative regulator of adipogenesis. First, CUL4B expression was downregulated during adipocyte differentiation in obese mice and was inversely correlated with BMI. Second, knockdown of CUL4B in 3T1-L1 cells led to increased adipocyte differentiation, whereas the overexpression of CUL4B had the opposite effect. Third, most importantly, the deletion of CUL4B in adipose tissues greatly facilitated adipogenesis. When challenged with HFD, AKO mice exhibited increased body weight gain and fat mass. Mechanistically, we demonstrated that the negative regulation of adipogenesis by CUL4B is mediated by the polyubiquitination of PPARγ, a master regulator of adipogenesis and insulin sensitivity. In particular, the treatment with PPARγ inhibitor GW9662 in HFD-fed AKO mice could efficiently block the increased adipogenesis and decreased ...
http://diabetes.diabetesjournals.org/content/66/2/300
development | AlleleBlogdevelopment | AlleleBlog
Title:. A Novel pro-adipogenesis factor abundant in adipose tissues and over-expressed in obesity acts upstream of PPARg and C/EBPa. Authors:. Yuhui Ni, Chenbo Ji, Bin Wang, Jie Qiu, Jiwu Wang, Xirong Guo Abstract:. An important question about adipogenesis is how master adipogenesis factors (defined as being able to initiate adipogenesis when expressed alone) peroxisome proliferator-activated receptor (PPAR) initiate adipogenesis only in differentiating preadipocytes. The objective of our research was to find previously unidentified factors that are unique or highly enriched in cells of the adipocyte lineage during adipogenesis that may provide functional tissue specificity to preadipocytes. We reasoned that such factors may alter expression profile specifically in obese individuals. Omental adipose tissues were obtained from obese and non-obese male patients undergoing emergency abdominal surgery. mRNAs extracted from either group were used for suppression subtraction hybridization (SSH). Genes ...
http://blog.allelebiotech.com/tag/development/
Quantification of Human Adipogenesis - No Study Results Posted - ClinicalTrials.govQuantification of Human Adipogenesis - No Study Results Posted - ClinicalTrials.gov
The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details ...
https://clinicaltrials.gov/ct2/show/results/NCT01257997
The combination of DHEA, histamine, and insulin increases adipogenic differentiation and enhances tissue transplantation...
TY - JOUR. T1 - The combination of DHEA, histamine, and insulin increases adipogenic differentiation and enhances tissue transplantation outcome in mice. AU - Park, Yoorim. AU - Jung, Min Kyung. AU - Yoon, Sun Young. AU - Lee, Ha Reum. AU - Hur, Dae Young. AU - Kim, Daejin. AU - Yang, Yoolhee. AU - Kim, Tae Sung. AU - Kim, Seonghan. AU - Yoon, Suk Ran. AU - Park, Hyun Jeong. AU - Bang, Sa Ik. AU - Cho, Dae Ho. PY - 2013/5/1. Y1 - 2013/5/1. N2 - Adipose stem cells (ASCs) are pluripotent cells that can generate pure fat tissue for regeneration. Differentiated adipose cells have been generated by a common inducer cocktail composed of dexamethasone, insulin, and isobutylmethylxanthine (DIM). The major drawbacks of adipose cells are their tendency to float on the culture media and their cost. To overcome some of these disadvantages, a new inducer cocktail that includes insulin, dehydroepiandrosterone, and histamine (DHIH) was tested. As a result, lipid accumulation was elevated more than twofold with ...
https://koreauniv.pure.elsevier.com/en/publications/the-combination-of-dhea-histamine-and-insulin-increases-adipogeni
Inhibition of Adipogenesis by Wnt Signaling | ScienceInhibition of Adipogenesis by Wnt Signaling | Science
Adipocytes arise from mesodermal stem cells, which have the capacity to differentiate into a variety of other cell types, including myocytes (1). Once committed to the adipocyte lineage, preadipocytes can remain quiescent, multiply, or undergo differentiation and become adipocytes. 3T3-L1 and 3T3-F442A cells are established mouse preadipocyte models. Both cell lines can be induced to differentiate in cell culture, but 3T3-F442A cells are thought to be arrested at a later point in development (2). Studies of these cellular models have revealed some of the molecular events that orchestrate adipogenesis, including the role of C/EBPs and PPARγ in mediating the expression of adipocyte-specific genes (3, 4).. Wnts are a family of paracrine and autocrine factors that regulate cell growth and cell fate (5). Signaling is initiated when Wnt ligands bind to transmembrane receptors of the Frizzled family. In the canonical Wnt signaling pathway, Frizzleds signal through Dishevelled to inhibit the kinase ...
https://science.sciencemag.org/content/289/5481/950?ijkey=1b84671ddd53ae05b86b4456f0c39ddd563ec201&keytype2=tf_ipsecsha
Inhibition of adipocyte differentiation by mechanical stretching through ERK-mediated downregulation of PPARγ2 | Journal of...Inhibition of adipocyte differentiation by mechanical stretching through ERK-mediated downregulation of PPARγ2 | Journal of...
The present results provide direct evidence for a regulatory role of mechanical stress in adipocyte differentiation, mediated through the activation of the ERK/MAPK system. Controversial observations concerning the role of ERK/MAPK in adipocyte differentiation have been reported by several laboratories - the activation of the ERK/MAPK pathway has been shown to be involved in both the inhibition (Font de Mora et al., 1997; Hu et al., 1996; Kim et al., 2001; Shimba et al., 2001) and the promotion (Bost et al., 2002; Klemm et al., 2001; Machinal-Quelin et al., 2002; Prusty et al., 2002; Zhang et al., 1996) of adipocyte differentiation. Along these lines, Prusty et al. recently suggested that stimulation of the ERK/MAPK pathway might have opposing effects in the process of adipogenesis, depending on the time of activation during the differentiation process (Prusty et al., 2002). In the present study, the activated state of ERK1/2 was more prolonged during the induction period in response to the ...
http://jcs.biologists.org/content/117/16/3605
Other Peptide Receptors - Small-molecule XIAP inhibitors derepress downstream effector caspasesOther Peptide Receptors - Small-molecule XIAP inhibitors derepress downstream effector caspases
People who have Type 2 diabetes mellitus (T2DM) have reduced bone LY2784544 tissue nutrient density and an elevated threat of fractures because of altered mesenchymal stem cell (MSC) differentiation in the bone tissue marrow. of metformin had been seen in multipotent C3H10T1/2 MSCs where metformin exerted reciprocal control over the actions of Runx2 as well as the adipogenic transcription aspect PPARγ resulting in suppression of adipogenesis. These effects were unbiased of AMPK activation but through the suppression from the mTOR/p70S6K signalling pathway rather. Basal AMPK and mTOR/p70S6K activity do seem to be necessary for adipogenesis as showed through the AMPK inhibitor substance C. This observation was additional supported through the use of AMPK knockout mouse embryo fibroblasts (MEFs) where adipogenesis as evaluated by decreased lipid deposition and expression from the adipogeneic transcription aspect C/EBPβ was discovered to display a total requirement of AMPK. Further activation of ...
https://rmrfotoarts.com/category/other-peptide-receptors/
Repressor transcription factor 7-like 1 promotes adipogenic competency in precursor cells | PNASRepressor transcription factor 7-like 1 promotes adipogenic competency in precursor cells | PNAS
Adipose tissue is a highly specialized compartment of cells actively involved in maintaining global metabolic homeostasis through lipid synthesis and storage, adipokine secretion, and insulin responsiveness (1). Adipocytes compose the majority of cells in adipose tissue and play a critical role in normal physiology, but their dysfunction is also at the center of a diverse range of diseases, including obesity, diabetes, and lipodystrophies (2). Furthermore, primary preadipocytes and adipose-derived stem cells have shown promise in treating multiple conditions (3-5). Therefore, it is critical to understand the process by which spindly fibroblastic precursor cells undergo conversion into round lipid-laden fat cells.. In vitro models of adipogenesis, such as the extensively studied committed preadipocyte cell line 3T3-L1 cells, have elucidated two major phases of adipogenesis: commitment and terminal differentiation (6, 7). Terminal differentiation is characterized by the induction of metabolic ...
https://www.pnas.org/content/108/39/16271
Search results | TNO PublicationsSearch results | TNO Publications
The effect of n-6 polyunsaturated fatty acids (n-6 PUFAs) on adipogenesis and obesity is controversial. Using in vitro cell culture models, we show that n-6 PUFAs was pro-adipogenic under conditions with base-line levels of cAMP, but anti-adipogenic when the levels of cAMP were elevated. The anti-adipogenic action of n-6 PUFAs was dependent on a ...
https://repository.tno.nl/islandora/search/subject%3A%22Mice%252C%255C%2BInbred%255C%2BC57BL%22
Early-Life Programming of Adipogenesis and Adiposity - Adipose Tissue in Health and Disease - Cripps - Wiley Online LibraryEarly-Life Programming of Adipogenesis and Adiposity - Adipose Tissue in Health and Disease - Cripps - Wiley Online Library
Cripps, R. L. and Ozanne, S. E. (2010) Early-Life Programming of Adipogenesis and Adiposity, in Adipose Tissue in Health and Disease (eds T. Leff and J. G. Granneman), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527629527.ch24 ...
http://onlinelibrary.wiley.com/doi/10.1002/9783527629527.ch24/references
Expression of Nuclear Receptors during Adipogenesis of 3T3-L1 Cells COUP-TFI COUP-TFII PPAR  VDR GCNF ROR  HNF4  LXR  LXR ...Expression of Nuclear Receptors during Adipogenesis of 3T3-L1 Cells COUP-TFI COUP-TFII PPAR  VDR GCNF ROR  HNF4  LXR  LXR ...
LXR α 0h 4h 8h 18h 1D 2D 3D 4D 5D 6D 7D 8D h 2d d Induction MediumDifferentiation Medium NR Expression during Adipogenesis of 3T3-L1 Cells (2) Fu et al., Mol. Endo. 19: 2437 (2005) Nur77 PPAR γ NUR77 LXR  PPAR 
http://slideplayer.com/slide/3347478/
Differential effects of fibroblast growth factor and tumor promoters on the initiation and maintenance of adipocyte...Differential effects of fibroblast growth factor and tumor promoters on the initiation and maintenance of adipocyte...
Fibroblast growth factor (FGF) has been shown to inhibit the differentiation of myogenic and adipogenic cell lines without inducing a proliferative response. We have previously shown that agents capable of activating protein kinase C (PKC), such as FGF and the phorbol ester tetradecanoyl phorbol-13-acetate (TPA), inhibit the differentiation of the adipogenic cell line TA1, as measured by the rapid loss of adipocyte-specific RNAs. We report here that the treatment of fully differentiated TA1 adipocytes with FGF at 10 ng/ml induces the reversal of adipocyte differentiation, even in cells where PKC activity has been down-regulated by TPA pretreatment. In contrast, TPA or lower concentrations of FGF (1 ng/ml), both effective inducers of c-fos RNA in adipocytes, fail to reverse adipocyte differentiation. The adipocytes, however, will extinguish differentiation-specific functions in response to TPA by the addition of a calcium ionophore. Therefore, we propose that there are two FGF-sensitive pathways ...
https://rupress.org/jcb/article/109/4/1857/28719/Differential-effects-of-fibroblast-growth-factor
JCI - Deciphering the cellular interplays underlying obesity-induced adipose tissue fibrosisJCI - Deciphering the cellular interplays underlying obesity-induced adipose tissue fibrosis
Adipose tissue progenitors (or precursors), often located in the vicinity of the vascular network, constitute a heterogeneous population. They can be discriminated through their capacity to differentiate into mature adipocytes and also by their level of commitment into the adipocyte differentiation program. The application of flow cytometry using various markers as well as single-cell RNA sequencing has enabled the identification of multiple cell populations. The CD9hi progenitors exhibited very limited adipogenic capacity with a high propensity for the production of extracellular matrix components. CD9hi progenitors include mesothelial cells, whose contribution in adipose tissue remodeling is currently unresolved. Further investigations are still needed to establish the relationship between these various populations of progenitors. In addition, a better understanding of the critical functional determinants and whether acquired phenotypes are reversible is needed ...
https://516shouji.com.mobile.jci.org/articles/view/129192/figure/1
Srujana Rayalam, PhDSrujana Rayalam, PhD
Dr. Rayalam has worked in the areas of obesity, body weight regulation, phytochemicals and adipocyte biochemistry for over 8 years. Her research interests include: 1) to study the adipocyte life cycle and to understand the interaction of adipocytes with other cell types as an approach to address several problems associated with obesity; 2) to develop novel treatment strategies for obesity by inducing transdifferentiation of white to beige adipocytes and to inhibit lipid accumulation in white adipocytes; and 3) to identify combinations of phytochemicals and vitamins that have synergistic anti-adipogenic effects with an ultimate goal of developing pharmaceuticals or nutraceuticals for prevention and treatment of obesity and associated disorders. Aging is accompanied by an accumulation of adipocytes in bone marrow and Dr. Rayalams other interest is to understand the fat-bone interaction and to identify molecular targets for the prevention of weight gain and bone loss associated with aging. Dr. ...
https://www.pcom.edu/academics/faculty/srujanara.html
Kindkes Scrap Notes: High glucose induces adipogenic differentiationKindkes Scrap Notes: High glucose induces adipogenic differentiation
I think this is part of the puzzle as to why refined carbs in particular can be so fattening. It is well documented that the digestibility of carbohydrates determines the corresponding postprandial blood sugar spike ( glycemic index ). In addition, I suppose you could say that, being insulin resistant in muscle leads to exaggerated and prolonged elevated postprandial glucose levels, and these high glucose concentrations ( could potentially ) activate adipogenic pathways, in both muscle and fat tissue ...
https://kindkehealthnotes.blogspot.com/2013/07/high-glucose-induces-adipogenic.html
Molecules and CellsMolecules and Cells
PPARγ plays a central role in regulating the expression of a wide range of genes involved in adipogenesis, lipid metabolism, insulin sensitivity, energy storage, inflammation and differentiation (Kroker and Bruning, 2015; Lefterova and Lazar, 2009). In particular, the crucial role of PPARγ in adipogenesis has been extensively studied by many researchers (Lefterova and Lazar, 2009). Although PPARγ functions as the master transcriptional regulator of adipocyte differentiation, few genes have been validated as direct PPARγ regulators or targets (Lefterova and Lazar, 2009). Thus, the identification of novel regulators of PPARγ is important for understanding the transcriptional regulatory mechanism of PPARγ in various cell types or tissues. PPARγ contains AF-1 and DBD in the N-terminal region, the flexible hinge domain in the middle, and LBD and AF-2 in the C-terminal region (Kroker and Bruning, 2015). Upon the binding of the PPARγ ligand, PPARγ forms a heterodimer with RXRα through their ...
http://www.molcells.org/journal/view.html?doi=10.14348/molcells.2020.0143&sort=start_Page
Pennington Biomedical Research CenterPennington Biomedical Research Center
Dr. Marlatt is interested in the role of dietary and exercise interventions to facilitate healthy aging and metabolic health as it relates to women, particularly in the transition through menopause. She currently is focusing her research efforts on the impact of a drug intervention in post-menopausal women; the impact of hormones and race on adipogenesis and adipocyte morphology; as well as intermittent hypoxia in individuals with diabetes ...
https://www.pbrc.edu/training-and-education/postdocs/?postdocid=192
Plus it
Pharmacological dosing of all-trans-retinoic acid (atRA) controls adiposity in rodents by inhibiting adipogenesis and inducing fatty acid oxidation. Retinol dehydrogenases (Rdh) catalyze the first reaction that activate retinol into atRA. This study examined post-natal contributions of Rdh10 to atRA biosynthesis and physiological functions of endogenous atRA. Embryonic fibroblasts from Rdh10 heterozygote hypomorphs or with a total Rdh10 knockout exhibit decreased atRA biosynthesis and escalated adipogenesis. atRA or a RAR pan-agonist reversed the phenotype. Eliminating one Rdh10 copy in vivo (Rdh10+/-) yielded a modest decrease (≤25%) in the atRA concentration of liver and adipose, but increased adiposity in male and female mice fed a high-fat diet, increased liver steatosis, glucose intolerance and insulin resistance in males fed a high-fat diet, and activated bone marrow adipocyte formation in females, regardless of dietary fat. Chronic dosing with low dose atRA corrected the metabolic ...
http://hw-f5-diabetes.highwire.org/content/early/2018/01/09/db17-0946
Conferința Structure-specific zinc adipogenesis. An in vitro structure-bioactivity correlation study in Diabetes mellitusConferința Structure-specific zinc adipogenesis. An in vitro structure-bioactivity correlation study in Diabetes mellitus
HUMAN EXCRETORY SYSTEM - ppt downloadHUMAN EXCRETORY SYSTEM - ppt download
carbon skeleton use as energy source or converted to glycogen or fat Formation of Urea urea Growth and repair O H synthesis NH3 (ammonia) HO C C NH2 R excess O H HO C C NH2 amino acid R carbon skeleton use as energy source or converted to glycogen or fat
http://slideplayer.com/slide/4249740/
Anti-Adipogenic | GreenMedInfo | Pharmacological Action | NaturalAnti-Adipogenic | GreenMedInfo | Pharmacological Action | Natural
By default, all articles on GreenMedInfo.com are sorted based on the content type which best reflects the data which most users are searching for. For instance, people viewing substances are generally most interested in viewing diseases that these substances have shown to have positive influences. This section is for allowing more advanced sorting methods. Currently, these advanced sorting methods are available for members only. If you are already a member, you can sign in by clicking here. If you do not currently have a user account, and would like to create one/become a member, click here to begin the singup process ...
http://www.greenmedinfo.com/pharmacological-action/anti-adipogenic
MORC2 Polyclonal Antibody, HRP Conjugated | EpiGentekMORC2 Polyclonal Antibody, HRP Conjugated | EpiGentek
Background Exhibits a cytosolic function in lipogenesis, adipogenic differentiation, and lipid homeostasis by increasing the activity of ACLY, possibly preventing its dephosphorylation. May act as a transcriptional repressor....
https://www.epigentek.com/catalog/morc2-polyclonal-antibody-hrp-conjugated-p-15182.html
rs10483032 - SNPediars10483032 - SNPedia
PMID 22527884] Genetic variation in the carbonyl reductase 3 gene confers risk of type 2 diabetes and insulin resistance: a potential regulator of adipogenesis ...
https://snpedia.com/index.php/Rs10483032
Dynamic upregulation of CD24 in pre-adipocytes promotes adipogenesis - Memorial University Research RepositoryDynamic upregulation of CD24 in pre-adipocytes promotes adipogenesis - Memorial University Research Repository
The development of mature adipocytes from pre-adipocytes is a highly regulated process. CD24 is a glycophosphatidylinositol-linked cell surface receptor that has been identified as a critical cell surface marker for identifying pre-adipocytes that are able to reconstitute white adipose tissue (WAT) in vivo. Here, we examined the role and regulation of CD24 during adipogenesis in vitro. We found that CD24 mRNA and protein expression is upregulated early during adipogenesis in the 3T3-L1 pre-adipocytes and in murine primary pre-adipocytes isolated from subcutaneous and visceral WAT, followed by downregulation in mature adipocytes. CD24 mRNA expression was found to be dependent on increased transcription due to increased promoter activity in response to activation of a preexisting transcriptional regulator. Furthermore, either intracellular cAMP or dexamethasone were sufficient to increase expression in pre-adipocytes, while both additively increased CD24 expression. Preventing the increase in CD24 ...
https://research.library.mun.ca/9790/
MCP-1 Induced Protein Promotes Adipogenesis via Oxidative Stress, Endo by Craig Younce and Pappachan KolattukudyMCP-1 Induced Protein Promotes Adipogenesis via Oxidative Stress, Endo by Craig Younce and Pappachan Kolattukudy
Obesity involves inflammation. MCP-1, an inflammatory chemokine, and MCP-1-induced protein (MCPIP) are known to induce adipogenesis that causes increase in the number of adipocytes. Here we elucidate the intermediate processes through which MCPIP induces adipogenesis. Forced expression of MCPIP in 3T3-L1 preadipocytes caused increased reactive oxygen/nitrogen species (ROS/RNS) production and inducible-nitric oxide synthase (iNOS) expression, endoplasnnic reticulum stress (ER), as indicated by expression of ER chaperones and protein disulfide isomerase, and autophagy as indicated by expression of beclin-1 and cleavage of LC3. Treatment of ROS inhibitor, apocynin attenuated MCPIP induction of adipogenesis as measured by the induction of transcription factors involved in adipogenesis, adipocyte markers and lipid droplet accumulation. Inhibition of ER stress with taurursodeoxycholate or knockdown of inositol requiring enzyme 1 (IRE1) inhibited MCPIP induced autophagy and adipogenesis. Preadipocytes in
https://stars.library.ucf.edu/facultybib2010/3538/
Proteomic Analysis of Bovine Longissimus Muscle Satellite Cells during Adipogenic DifferentiationProteomic Analysis of Bovine Longissimus Muscle Satellite Cells during Adipogenic Differentiation
Satellite cells are skeletal muscle progenitor/stem cells that reside between the basal lamina and plasma membranes of skeletal fibers in vivo. These cells can give rise to both myogenic and adipogenic cells. Given the possible role for differentiation of satellite cells into adipocytes in marbling and in some pathological disorders like sarcopenia, knowledge of the proteins involved in such process remains obscure. Using two-dimensional polyacrylamide gel electrophoresis coupled with mass spectrometry, we investigated the proteins that are differentially expressed during adipogenic differentiation of satellite cells from bovine longissimus muscle. Our proteome mapping strategy to identify the differentially expressed intracellular proteins during adipogenic differentiation revealed a total of 25 different proteins. The proteins up-regulated during adipogenic differentiation of satellite cells like Cathepsin H precursor, Retinal dehydrogenase 1, Enoyl-CoA hydratase, Ubiquinol-cytochrome-c ...
https://www.ajas.info/journal/view.php?number=22508
Decreased RB1 mRNA, Protein, and Activity Reflect Obesity-Induced Altered Adipogenic Capacity in Human Adipose TissueDecreased RB1 mRNA, Protein, and Activity Reflect Obesity-Induced Altered Adipogenic Capacity in Human Adipose Tissue
eng] Retinoblastoma (Rb1) has been described as an essential player in white adipocyte differentiation in mice. No studies have been reported thus far in human adipose tissue or human adipocytes. We aimed to investigate the possible role and regulation of RB1 in adipose tissue in obesity using human samples and animal and cell models. Adipose RB1 (mRNA, protein, and activity) was negatively associated with BMI and insulin resistance (HOMA-IR) while positively associated with the expression of adipogenic genes (PPARγ and IRS1) in both visceral and subcutaneous human adipose tissue. BMI increase was the main contributor to adipose RB1 downregulation. In rats, adipose Rb1 gene expression and activity decreased in parallel to dietary-induced weight gain and returned to baseline with weight loss. RB1 gene and protein expression and activity increased significantly during human adipocyte differentiation. In fully differentiated adipocytes, transient knockdown of Rb1 led to loss of the adipogenic ...
https://dspace.uib.es/xmlui/handle/11201/152113
Effect of germinated brown rice extracts on pancreatic lipase, adipogenesis and lipolysis in 3T3-L1 adipocytes<...
TY - JOUR. T1 - Effect of germinated brown rice extracts on pancreatic lipase, adipogenesis and lipolysis in 3T3-L1 adipocytes. AU - See Meng, Lim. AU - Goh, Yong Meng. AU - Kuan, Wen Bin. AU - Loh, Su Peng. PY - 2014/11/3. Y1 - 2014/11/3. N2 - Background: This study investigated anti-obesity effects of seven different solvent (n-hexane, toluene, dicholoromethane, ethyl acetate, absolute methanol, 80% methanol and deionized water) extracts of germinated brown rice (GBR) on pancreatic lipase activity, adipogenesis and lipolysis in 3T3-L1 adipocytes. Methods: GBR were extracted separately by employing different solvents with ultrasound-assisted. Pancreatic lipase activity was determined spectrophotometrically by measuring the hydrolysis of p-nitrophenyl butyrate (p-NPB) to p-nitrophenol at 405 nm. Adipogenesis and lipolysis were assayed in fully differentiated 3T3-L1 adipocytes by using Oil Red O staining and glycerol release measurement. Results: GBR extract using hexane showed the highest ...
https://ukm.pure.elsevier.com/en/publications/effect-of-germinated-brown-rice-extracts-on-pancreatic-lipase-adi
The retinoblastoma-histone deacetylase 3 complex inhibits PPARgamma and adipocyte differentiationThe retinoblastoma-histone deacetylase 3 complex inhibits PPARgamma and adipocyte differentiation
The retinoblastoma protein (RB) has previously been shown to facilitate adipocyte differentiation by inducing cell cycle arrest and enhancing the transactivation by the adipogenic CCAAT/enhancer binding proteins (C/EBP). We show here that the peroxisome proliferator-activated receptor gamma (PPARgamma), a nuclear receptor pivotal for adipogenesis, promotes adipocyte differentiation more efficiently in the absence of RB. PPARgamma and RB were shown to coimmunoprecipitate, and this PPARgamma-RB complex also contains the histone deacetylase HDAC3, thereby attenuating PPARgammas capacity to drive gene expression and adipocyte differentiation. Dissociation of the PPARgamma-RB-HDAC3 complex by RB phosphorylation or by inhibition of HDAC activity stimulates adipocyte differentiation. These observations underscore an important function of both RB and HDAC3 in fine-tuning PPARgamma activity and adipocyte differentiation.. Keywords: Thiazolidinediones. ...
https://infoscience.epfl.ch/record/135337
Beta-mecaptoethanol suppresses inflammation and induces adipogenic differentiation in 3T3-F442A murine preadipocytes<...
TY - JOUR. T1 - Beta-mecaptoethanol suppresses inflammation and induces adipogenic differentiation in 3T3-F442A murine preadipocytes. AU - Guo, Wen. AU - Li, Yahui. AU - Liang, Wentao. AU - Wong, Siu. AU - Apovian, Caroline. AU - Kirkland, James L.. AU - Corkey, Barbara E.. PY - 2012/7/23. Y1 - 2012/7/23. N2 - Preadipocytes are present in adipose tissues throughout adult life that can proliferate and differentiate into mature adipocytes in response to environmental cues. Abnormal increase in adipocyte number or size leads to fat tissue expansion. However, it is now recognized that adipocyte hypertrophy is a greater risk factor for metabolic syndrome whereas fat tissue that continues to produce newer and smaller fat cells through preadipocyte differentiation is metabolically healthy. Because adipocyte hypertrophy is often associated with increased oxidant stress and low grade inflammation, both are linked to disturbed cellular redox, we tested how preadipocyte differentiation may be regulated ...
https://mayoclinic.pure.elsevier.com/en/publications/beta-mecaptoethanol-suppresses-inflammation-and-induces-adipogeni
CD90 serves as differential modulator of subcutaneous and visceral adipose-derived stem cells by regulating AKT activation that...CD90 serves as differential modulator of subcutaneous and visceral adipose-derived stem cells by regulating AKT activation that...
White adipose tissue includes subcutaneous and visceral adipose tissue (SAT and VAT) with different metabolic features. SAT protects from metabolic disorders, while VAT promotes them. The proliferative and adipogenic potentials of adipose-derived stem cells (ADSCs) are critical for maintaining adipose tissue homeostasis through driving adipocyte hyperplasia and inhibiting pathological hypertrophy. However, it remains to be elucidated the critical molecules that regulate different potentials of subcutaneous and visceral ADSCs (S-ADSCs, V-ADSCs) and mediate distinct metabolic properties of SAT and VAT. CD90 is a glycosylphosphatidylinositol-anchored protein on various cells, which is also expressed on ADSCs. However, its expression patterns and differential regulation on S-ADSCs and V-ADSCs remain unclear. S-ADSCs and V-ADSCs were detected for CD90 expression. Proliferation, colony formation, cell cycle, mitotic clonal expansion, and adipogenic differentiation were assayed in S-ADSCs, V-ADSCs, or CD90
https://stemcellres.biomedcentral.com/articles/10.1186/s13287-019-1459-7/figures/4
In todays study we investigated the consequences of genistein on adipogenic | Application of Computational Methods for the...In todays study we investigated the consequences of genistein on adipogenic | Application of Computational Methods for the...
In todays study we investigated the consequences of genistein on adipogenic differentiation of mouse bone tissue marrow-derived mesenchymal stem cell (BMSC) cultures and its own potential signaling pathway. differentiation. Genistein decreased the phosphorylation of ERK1/2 in mouse BMSC ethnicities dose-dependently. Genistein incubation for the whole tradition period in adition to that applied through the early stage of the tradition period considerably inhibited Rabbit Polyclonal to Cox1. Vorinostat the adipogenic Vorinostat differentiation of mouse BMSC ethnicities. While genistein was incubated in the past due stage (after day time 9) no inhibitory influence on adipogenic differentiation was noticed. BMSC ethnicities treated with genistein in the current presence of fibroblast growth element-2 (FGF-2) an activator from the ERK1/2 signaling pathway indicated normal degrees of ERK1/2 activity and by doing this can handle going through adipogenesis. Our outcomes claim Vorinostat that activation ...
http://world-of-links.com/in-todays-study-we-investigated-the-consequences-of-genistein-on-adipogenic/
Dynamic complexes of A-type lamins and emerin influence adipogenic capacity of the cell via nucleocytoplasmic distribution of β...Dynamic complexes of A-type lamins and emerin influence adipogenic capacity of the cell via nucleocytoplasmic distribution of β...
Marian Blanca Ramírez from the CSIC in Spain has been studying the effects of LRRK2, a protein associated with Parkinsons disease, on cell motility. A Travelling Fellowship from Journal of Cell Science allowed her to spend time in Prof Maddy Parsons lab at Kings College London, learning new cell migration assays and analysing fibroblasts cultured from individuals with Parkinsons. Read more on her story here. Where could your research take you? The deadline to apply for the current round of Travelling Fellowships is 30 Nov 2017. Apply now!. ...
http://jcs.biologists.org/content/early/2009/01/06/jcs.026179
OPUS 4 | SearchOPUS 4 | Search
Artificial adipose tissue (AT) constructs are urgently needed to treat severe wounds, to replace removed tissue, or for the use as in vitro model to screen for potential drugs or study metabolic pathways. The clinical translation of products is mostly prevented by the absence of a vascular component that would allow a sustainable maintenance and an extension of the construct to a relevant size. With this study, we aimed to evaluate the suitability of a novel material based on bacterial cellulose (CBM) on the defined adipogenic differentiation of human adipose-derived stem cells (ASCs) and the maintenance of the received adipocytes (diffASCs) and human microvascular endothelial cells (mvECs) in mono- and coculture. A slight acceleration of adipogenic differentiation over regular tissue culture polystyrene (TCPS) was seen on CBM under defined conditions, whereas on the maintenance of the generated adipocytes, comparable effects were detected for both materials. CBM facilitated the formation of ...
https://publikationen.reutlingen-university.de/solrsearch/index/search/searchtype/authorsearch/author/Petra+Kluger/rows/20/start/0/author_facetfq/Hack%2C+Larissa/yearfq/2019
Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose...Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose...
הבידול של adipocytes לבן ובייז של רקמת שומן אבות כלי הדם נושא פוטנציאל שיפור חילוף החומרים ההשמנה. נתאר פרוטוקולים של CD34 + CD31 +...
https://www.jove.com/v/57804?language=Hebrew
Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose...Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose...
Yağ dokusu vasküler ataları gelen beyaz ve bej adiposit farklılaşma obezite metabolik iyileştirme için potansiyel taşımaktadır. CD34 +...
https://www.jove.com/video/57804/yaltm-genileme-ve-adipojenik-indksiyon-cd34-cd31-insan-omental-ve?language=Turkish
Human Preadipocytes-subcutaneous | Creative BioarrayHuman Preadipocytes-subcutaneous | Creative Bioarray
Adipocytes play an important role in energy storage and metabolism. Adipocyte differentiation is a developmental process that is critical for metabolic homeostasis and nutrient signaling. It is controlled by complex actions involving gene expression and signal transduction. Preadipocytes are present throughout adult life in adipose tissues and can proliferate and differentiate into mature adipocytes according to the energy balance. The proliferation and differentiation of these preadipocytes contribute to increases in adipose tissue mass. In vitro study indicates that different tissue-derived preadipocytes exhibit differently in lipid accumulation, adipogenic transcription factor expression, and TNF?-induced apoptosis. It has also been demonstrated that there is a close relationship between adipocyte differentiation and many physiological and pathological processes including fat metabolism, energy balance, obesity, diabetes, hyperlipidemia and breast cancer. HPA-s from Bioarray Research ...
https://www.creative-bioarray.com/Human-Preadipocytes-subcutaneous-CSC-7736W-item-1791.htm
Identification of potential specific biomarkers and key signaling pathways between osteogenic and adipogenic differentiation of...Identification of potential specific biomarkers and key signaling pathways between osteogenic and adipogenic differentiation of...
The differentiation of bone mesenchymal stem cells (BMSCs) into adipogenesis (AD) rather than osteogenesis (OS) is an important pathological feature of osteoporosis. Illuminating the detailed mechanisms of the differentiation of BMSCs into OS and AD would contribute to the interpretation of osteoporosis pathology. To identify the regulated mechanism in lineage commitment of the BMSCs into OS and AD in the early stages, the gene expression profiles with temporal series were downloaded to reveal the distinct fates when BMSCs adopt a committed lineage. For both OS and AD lineages, the profiles of days 2-4 were compared with day 0 to screen the differentially expressed genes (DEGs), respectively. Next, the functional enrichment analysis was utilized to find out the biological function, and protein-protein interaction network to predict the central genes. Finally, experiments were performed to verify our finding. FoxO signaling pathway with central genes like FoxO3, IL6, and CAT is the crucial mechanism of
https://josr-online.biomedcentral.com/articles/10.1186/s13018-020-01965-3
PLSCR3 (phospholipid scramblase 3 Scr3) is one of the superfamily of - Small Molecule Inhibitors of Protein Arginine...PLSCR3 (phospholipid scramblase 3 Scr3) is one of the superfamily of - Small Molecule Inhibitors of Protein Arginine...
PLSCR3 (phospholipid scramblase 3 Scr3) is one of the superfamily of membrane-associated transcription regulators named Tubby-like protein (TULPs). medium LAQ824 by means of extracellular microvesicles (exosomes). Alternatively Scr3 expression didnt decrease as well as the secretion of Scr3 significantly?in 3T3 Swiss-albino fibroblasts (a parental cell-line of 3T3-L1) had not been increased by differentiation treatment. Overexpression of human being Scr3 during 3T3-L1 differentiation suppressed triacylglycerol build up and inhibited induction from the mRNAs lately stage pro-adipogenic transcription elements [CCAAT/enhancer-binding proteins α (C/EBPα) and peroxisome proliferator-activated receptor γ (PPARγ)] and X-box-binding proteins 1 (XBP1). Manifestation of early stage pro-adipogenic transcription elements (C/EBPβ and C/EBPδ) had not been considerably affected. These outcomes claim that Scr3 features as a poor regulator of adipogenesis in 3T3-L1 cells at a particular differentiation ...
http://welbourneprimary.com/plscr3-phospholipid-scramblase-3-scr3-is-one-of-the-superfamily-of/
Medical Advisor Journals, Kyle J. Norton Site, Health Tips for Better Living and Living Health: The Effects of Hormone...Medical Advisor Journals, Kyle J. Norton Site, Health Tips for Better Living and Living Health: The Effects of Hormone...
Adipocytes and fat cells play critical roles in the regulation of energy homeostasis. Adipogenesis (adipocyte differentiation) is regulated via a complex process including coordinated changes in hormone sensitivity and gene expression. According to the study by the Osaka University of Pharmaceutical Sciences, Prostaglandins (PGs), which are lipid mediators, are associated with the regulation of PPARγ function in adipocytes. Prostacyclin promotes the differentiation of adipocyte-precursor cells to adipose cells via activation of the expression of C/EBPβ and δ. These proteins are important transcription factors in the activation of the early phase of adipogenesis, and they activate the expression of PPARγ, which event precedes the maturation of adipocytes. PGE(2) and PGF(2α) strongly suppress the early phase of adipocyte differentiation by enhancing their own production via receptor-mediated elevation of the expression of cycloxygenase-2, and they also suppress the function of PPARγ(24). ...
https://kylejnorton.blogspot.ca/2013/12/the-effects-of-hormone-prostaglandins3.html
Cdk6 blocks myeloid differentiation by interfering with Runx1 DNA binding and Runx1‐C/EBPα interaction | The EMBO Journal
Coordinating terminal differentiation and cell cycle arrest involves coupling the activity of the transcriptional regulators that activate lineage‐specific gene expression programs to the cell cycle machinery. The importance of such coordination is illustrated by the observation that ectopic expression of cell cycle promoting factors is able to interfere with differentiation of numerous cell types. Well‐characterized examples include the ability of the c‐Myc oncoprotein to block the differentiation of adipocytes by repressing the transcription of C/EBPα, a key inducer of adipogenesis (Freytag and Geddes, 1992), and the ability of Cyclin D1/Cdk4 to inhibit myogenesis through binding to MyoD (Zhang et al, 1999). However, in other cases, the molecular mechanisms are not clear. E2F‐1 can block granulopoiesis (Strom et al, 1998), adipogenesis (Porse et al, 2001) and myogenesis (Wang et al, 1995), but the relevant molecular targets are not defined. Cdk6 inhibits osteogenic differentiation by ...
http://emboj.embopress.org/content/26/9/2361
Acquisition of human alveolar bone-derived stromal cells using minimally irrigated implant osteotomy: in vitro and in vivo...Acquisition of human alveolar bone-derived stromal cells using minimally irrigated implant osteotomy: in vitro and in vivo...
Bone chips were obtained by minimally irrigated implant drilling technique from 10 human donors. Isolated cells were studied with respect to their colony-forming efficiency, surface marker expression by immunofluorescence staining, fluorescence-activated cell sorting analysis and self-renewal potency. To verify the differentiation activity, in vitro osteogenic and adipogenic gene expressions were evaluated by reverse transcription-polymerase chain reaction, and in vitro formation of mineralized nodule and adipocytes was also evaluated. In vivo bone-forming activity was assessed by ectopic transplantation in immunocompromised mice (n = 5 ...
https://pesquisa.bvsalud.org/odontologia/resource/pt/mdl-22420633
Plus itPlus it
The number of overweight and obese individuals continues to increase in both the U.S. and worldwide. This increase has led to a significant increase in obesity-related medical problems including diabetes mellitus, cardiovascular disease and cancer. In obesity, the differentiation of adipocytes is suppressed. Although adipocyte differentiation is associated with changes in glucose metabolism, little is known about the potential of enzymes involved in glucose metabolism to modulate this process. Pyruvate kinase (PK) mediates the rate-limiting step of glycolysis. The M2 isoform of PK (PKM2) is expressed in adipocytes but its role in adipogenesis is unknown. Here we demonstrate that PKM2 regulates the differentiation of both human and mouse adipocytes. Silencing of PKM2 in preadipocytes led to increased lipid accumulation, enhanced expression of markers (FABP4, PPARgamma, C/EBPBeta) of adipocyte differentiation and caused a shift in the pattern of enzymes involved in glucose metabolism favoring the ...
http://cancerpreventionresearch.aacrjournals.org/content/8/10_Supplement/B36
Evidence of impaired adipogenesis in insulin resistanceEvidence of impaired adipogenesis in insulin resistance
To elucidate the roles of adipose tissue and skeletal muscle in the early development of insulin resistance, we characterized gene expression profiles of isolated adipose cells and skeletal muscle of non-diabetic insulin-resistant first-degree relatives of type 2 diabetic patients using oligonucleot …
https://pubmed.ncbi.nlm.nih.gov/15094374/?dopt=Abstract
The Other Side of Life 24/7: ScienceDaily: Top Health News
A research team has managed to decode the process of adipogenesis by identifying the precise proteins that play the leading roles in fat absorption. There are many actors involved in the process of adipogenesis, used by the body to store the fat that it absorbs from food. Up to now there had been some uncertainty as to how it was regulated. Yet, understanding this mechanism is of crucial importance to prevent the diseases related to fat accumulation in adipose tissue ...
http://wyld-side.blogspot.com/2014/10/sciencedaily-top-health-news_3.html
Evaluation Of Properties On Controlling The Adipogenesis Process In Citrullus Colocynthis Seed Extracts - Free Essay Example |...Evaluation Of Properties On Controlling The Adipogenesis Process In Citrullus Colocynthis Seed Extracts - Free Essay Example |...
Obesity is a growing epidemic around the world and dramatically increases the risk of developing a number of chronic diseases (Gambero and Ribeiro, 2015).
https://eduzaurus.com/free-essay-samples/evaluation-of-properties-on-controlling-the-adipogenesis-process-in-citrullus-colocynthis-seed-extracts/
A high-throughput, image-based screen to identify kinases involved in brown adipocyte development | Science SignalingA high-throughput, image-based screen to identify kinases involved in brown adipocyte development | Science Signaling
Thank you for your interest in spreading the word about Science Signaling.. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.. ...
http://stke.sciencemag.org/content/10/466/eaaf5357.full
Oil Red O Lipid Stain for adipocites and neutral trigliceridesOil Red O Lipid Stain for adipocites and neutral triglicerides
Oil Red O Lipid Stain kit is used to demonstrate adipocites and neutral triglicerides. This is also useful to demonstrate adipogenesis. Fat cells and neutral fat will be coloured in red and the nuclei in blue.
https://www.gbiosciences.com/Histology_Cytology_Reagents/Histological_Stains/Oil_Red_O_Lipid_Stain
Creb3l4-KO mice showed adipocyte hyperplasia, lead to i | Open-iCreb3l4-KO mice showed adipocyte hyperplasia, lead to i | Open-i
Creb3l4-KO mice showed adipocyte hyperplasia, lead to improved metabolic parameters. (a) Adipogenic potential of mouse embryonic fibroblasts (MEFs) derived from
https://openi.nlm.nih.gov/detailedresult.php?img=PMC4260748_cddis2014490f9&req=4
Characteristics of human MSCs. (A) Cells in culture on | Open-iCharacteristics of human MSCs. (A) Cells in culture on | Open-i
Characteristics of human MSCs. (A) Cells in culture on day 3 (left) and day 8 (right) from subculture from passage 4. (B) Adipogenic differentiation of human MS
https://openi.nlm.nih.gov/detailedresult.php?img=PMC4373437_jcmm0015-2684-f1&req=4
CiNii 論文 - Cryptic fragment α4 LG4-5 derived from laminin α4 chain inhibits de novo...
Cryptic fragment α4 LG4-5 derived from laminin α4 chain inhibits de novo adipogenesis by modulating the effect of fibroblast growth factor-2 ...
https://ci.nii.ac.jp/naid/10021095600
Dual functions of TAF7L in adipocyte differentiation | eLifeDual functions of TAF7L in adipocyte differentiation | eLife
A combination of cellular, biochemical, genetic and genomic techniques have revealed a new molecular player in the production of fat cells in mice, which could improve our understanding of obesity.
https://elifesciences.org/articles/00170
AdipogenesisAdipogenesis
... and triiodothyronine effectively induce adipogenesis in preadipocytes. Insulin regulates adipogenesis through insulin-like ... Reduced adipogenesis in obese persons is due to increased senescent cells in adipose tissue rather than reduced numbers of stem ... Adipogenesis is the formation of adipocytes (fat cells) from stem cells. It involves 2 phases, determination, and terminal ... cAMP, an inducer of adipogenesis, can promote expression of C/EBPβ and C/EBPδ. At the early stage of differentiation, the ...
https://en.wikipedia.org/wiki/Adipogenesis
Forkhead box protein O1Forkhead box protein O1
The failure to commit to adipogenesis is primarily due to active FOXO1 arresting the cell in G0/G1 through activation of yet ... FOXO1 negatively regulates adipogenesis. Presently, the exact mechanism by which this is accomplished is not entirely ... Rising levels of PPARG are required to initiate adipogenesis; by preventing its transcription, FOXO1 is preventing the onset of ... In the currently accepted model, FOXO1 negatively regulates adipogenesis by binding to the promoter sites of PPARG and ...
https://en.wikipedia.org/wiki/Forkhead_box_protein_O1
FirreFirre
It plays a role in pluripotency and adipogenesis. Long non-coding RNA GRCh38: Ensembl release 89: ENSG00000213468 - Ensembl, ... "Long noncoding RNAs regulate adipogenesis". Proceedings of the National Academy of Sciences of the United States of America. ...
https://en.wikipedia.org/wiki/Firre
MicroRNAMicroRNA
Studies to determine what role pluripotent stem cells play in adipogenesis, were examined in the immortalized human bone marrow ... Romao JM, Jin W, Dodson MV, Hausman GJ, Moore SS, Guan LL (September 2011). "MicroRNA regulation in mammalian adipogenesis". ... Conversely, ectopic expression of the miRNAs 155, 221, and 222 significantly inhibited adipogenesis and repressed induction of ... alpha expression by C/EBP beta during adipogenesis requires a peroxisome proliferator-activated receptor-gamma-associated ...
https://en.wikipedia.org/wiki/MicroRNA
Yihai CaoYihai Cao
Cao, Yihai (2007-09-01). "Angiogenesis modulates adipogenesis and obesity". The Journal of Clinical Investigation. 117 (9): ...
https://en.wikipedia.org/wiki/Yihai_Cao
LipoblastLipoblast
Adipogenesis Adipose differentiation-related protein Lipoblastoma List of human cell types derived from the germ layers Michael ... Dani C (1999). "Embryonic stem cell-derived adipogenesis". Cells Tissues Organs (Print). 165 (3-4): 173-80. doi:10.1159/ ...
https://en.wikipedia.org/wiki/Lipoblast
DEPTORDEPTOR
... cell-autonomously regulates adipogenesis. In the muscle, Baf60c promotes a switch from oxidative to glycolytic myofiber ... "DEPTOR cell-autonomously promotes adipogenesis, and its expression is associated with obesity". Cell Metabolism. 16 (2): 202-12 ...
https://en.wikipedia.org/wiki/DEPTOR
WNT10BWNT10B
2000). "Inhibition of adipogenesis by Wnt signaling". Science. 289 (5481): 950-3. Bibcode:2000Sci...289..950R. doi:10.1126/ ... a molecular switch that governs adipogenesis. Gain-of-function of Wnt10b in mouse hearts has shown to improve cardiac tissue ...
https://en.wikipedia.org/wiki/WNT10B
GPR 120GPR 120
... is also a key regulator of adipogenesis, including adipocyte development and differentiation. Oh, Da Young; Talukdar, ... 2007). "The regulation of adipogenesis through GPR120". Biochemical and Biophysical Research Communications. 354 (2): 591-7. ...
https://en.wikipedia.org/wiki/GPR_120
STC1STC1
Serlachius M, Andersson LC (Jun 2004). "Upregulated expression of stanniocalcin-1 during adipogenesis". Experimental Cell ...
https://en.wikipedia.org/wiki/STC1
Let-7 microRNA precursorLet-7 microRNA precursor
Sun T, Fu M, Bookout AL, Kliewer SA, Mangelsdorf DJ (2009). "MicroRNA let-7 Regulates 3T3-L1 Adipogenesis". Mol Endocrinol. 23 ...
https://en.wikipedia.org/wiki/Let-7_microRNA_precursor
NEDD8NEDD8
PPARγ has a crucial role in adipogenesis and lipid accumulation within adipocytes (fat cells). Activated NEDD8 stabilizes PPARγ ... allowing increased adipogenesis. In experiments with mice, Pevonedistat, a drug inhibiting activation of NEDD8, prevented high- ... "PPARγ neddylation essential for adipogenesis is a potential target for treating obesity". Cell Death and Differentiation. 23 (8 ...
https://en.wikipedia.org/wiki/NEDD8
ADAM12ADAM12
"ADAM 12 protease induces adipogenesis in transgenic mice". Am. J. Pathol. 160 (5): 1895-903. doi:10.1016/S0002-9440(10)61136-4 ...
https://en.wikipedia.org/wiki/ADAM12
CycloleucineCycloleucine
In a 2015 study on the role of N6-methyladenosine (m6A) demethylation on adipogenesis, researchers treated porcine adipocytes ... Wang, Xinxia; Zhu, Linna; Chen, Jingqing; Wang, Yizhen (2015-04-03). "mRNA m6A Methylation Downregulates Adipogenesis In ...
https://en.wikipedia.org/wiki/Cycloleucine
FollistatinFollistatin
August 2009). "Role of follistatin in promoting adipogenesis in women". The Journal of Clinical Endocrinology and Metabolism. ...
https://en.wikipedia.org/wiki/Follistatin
N6-MethyladenosineN6-Methyladenosine
"FTO influences adipogenesis by regulating mitotic clonal expansion". Nature Communications. 6: 6792. Bibcode:2015NatCo...6.6792 ... "FTO-dependent demethylation of N6-methyladenosine regulates mRNA splicing and is required for adipogenesis". Cell Research. 24 ...
https://en.wikipedia.org/wiki/N6-Methyladenosine
Agouti-signaling proteinAgouti-signaling protein
"Adipogenesis and obesity: rounding out the big picture". Cell. 87 (3): 377-89. doi:10.1016/S0092-8674(00)81359-8. PMID 8898192 ...
https://en.wikipedia.org/wiki/Agouti-signaling_protein
OxylipinOxylipin
Barquissau V, Ghandour RA, Ailhaud G, Klingenspor M, Langin D, Amri EZ, Pisani DF (2017). "Control of adipogenesis by oxylipins ...
https://en.wikipedia.org/wiki/Oxylipin
CathepsinCathepsin
Mouse cathepsin L is homologous to human cathepsin V. Mouse cathepsin L has been shown to play a role in adipogenesis and ... "Cathepsin L activity controls adipogenesis and glucose tolerance". Nat. Cell Biol. 9 (8): 970-7. doi:10.1038/ncb1623. PMC ...
https://en.wikipedia.org/wiki/Cathepsin
Jeffrey FlierJeffrey Flier
Cited 1373 times, June 2022 Spiegelman, B. M.; Flier, J. S. (1996-11-01). "Adipogenesis and obesity: rounding out the big ...
https://en.wikipedia.org/wiki/Jeffrey_Flier
KMT2DKMT2D
KMT2C and KMT2D are essential for adipogenesis and myogenesis. Similar functions are seen in neuronal and osteoblast ... "MLL3/MLL4 are required for CBP/p300 binding on enhancers and super-enhancer formation in brown adipogenesis". Nucleic Acids ... recruits and requires KMT2D to establish a subset of adipogenic enhancers during adipogenesis. Depletion of KMT2D prior to ...
https://en.wikipedia.org/wiki/KMT2D
OprelvekinOprelvekin
Synonyms are: AGIF Adipogenesis inhibitory factor Interleukin-11 precursor. Oprelvekin is produced in Escherichia coli (E. coli ... the inhibition of adipogenesis, the induction of acute phase protein synthesis (e.g., fibrinogen), and inhibition of ...
https://en.wikipedia.org/wiki/Oprelvekin
Sodium pyruvateSodium pyruvate
"Exogenous Sodium Pyruvate Stimulates Adipogenesis of 3T3-L1 Cells". Journal of Cellular Biochemistry. 117 (1): 39-48. doi: ...
https://en.wikipedia.org/wiki/Sodium_pyruvate
Super-enhancerSuper-enhancer
"Molecular architecture of transcription factor hotspots in early adipogenesis". Cell Reports. 7 (5): 1434-42. doi:10.1016/j. ...
https://en.wikipedia.org/wiki/Super-enhancer
Acetyl-CoA carboxylaseAcetyl-CoA carboxylase
"A biotin analog inhibits acetyl-CoA carboxylase activity and adipogenesis". The Journal of Biological Chemistry. 277 (19): ...
https://en.wikipedia.org/wiki/Acetyl-CoA_carboxylase
Bone morphogenetic proteinBone morphogenetic protein
BMPs are also involved in adipogenesis and functional regulation of adipose tissue. BMP4 favors white adipogenesis, whereas ...
https://en.wikipedia.org/wiki/Bone_morphogenetic_protein
MiR-27MiR-27
Lin Q, Gao Z, Alarcon RM, Ye J, Yun Z (2009). "A role of miR-27 in the regulation of adipogenesis". FEBS J. 276 (8): 2348-58. ... acting by blocking the expression of two main regulators of adipogenesis. MicroRNAs miR-27a and -27b have been found to ...
https://en.wikipedia.org/wiki/MiR-27
ChemerinChemerin
... a new adipokine that modulates adipogenesis via its own receptor". Biochem. Biophys. Res. Commun. 362 (4): 1013-8. doi:10.1016/ ... a novel adipokine that regulates adipogenesis and adipocyte metabolism". J. Biol. Chem. 282 (38): 28175-88. doi:10.1074/jbc. ...
https://en.wikipedia.org/wiki/Chemerin
CMKLR1CMKLR1
As an adipokine receptor it has a role in adipogenesis and adipocyte maturation. It seems also to have a role in peripheral ... a novel adipokine that regulates adipogenesis and adipocyte metabolism". The Journal of Biological Chemistry. 282 (38): 28175- ...
https://en.wikipedia.org/wiki/CMKLR1
ObesogenObesogen
Janesick A, Blumberg B (March 2011). "Endocrine disrupting chemicals and the developmental programming of adipogenesis and ... "Endocrine-disrupting organotin compounds are potent inducers of adipogenesis in vertebrates" (PDF). Mol. Endocrinol. 20 (9): ... Monoethyl-hexyl-phthalate Is a Selective Peroxisome Proliferator-activated Receptor γ Modulator That Promotes Adipogenesis". J ...
https://en.wikipedia.org/wiki/Obesogen
Chemerin--a new adipokine that modulates adipogenesis via its own receptorChemerin--a new adipokine that modulates adipogenesis via its own receptor
... Biochem Biophys Res Commun. 2007 Nov 3;362(4):1013-8 ...
https://pubmed.ncbi.nlm.nih.gov/17767914/?dopt=Abstract
NOVEL ROLES OF THE CILIARY GENES, THM1 AND THM2, IN ADIPOGENESIS, SKELETAL DEVELOPMENT, AND SPERMATOGENESISNOVEL ROLES OF THE CILIARY GENES, THM1 AND THM2, IN ADIPOGENESIS, SKELETAL DEVELOPMENT, AND SPERMATOGENESIS
... ... shRNA-mediated Thm1 knockdown in 3T3-L1 mouse pre-adipocytes reveals Thm1 deficiency increases adipogenesis and insulin ... Thus, our studies establish novel roles for Thm1 and Thm2 in adipogenesis, skeletal development, and spermatogenesis and ...
https://kuscholarworks.ku.edu/handle/1808/27752
Role of murine macrophage in temporal regulation of cortisol- and serotonin-induced adipogenesis in pre-adipocytes when grown...Role of murine macrophage in temporal regulation of cortisol- and serotonin-induced adipogenesis in pre-adipocytes when grown...
Regulation of adipogenesis, the root cause for obesity, is very poorly understood. However, studies have presented evidence of ... Kinetic profile of adipogenesis genes. (A) Differentially expressed genes in co-cultured adipocytes treated with cortisol, ... Kinetic profile of adipogenesis genes. (A) Differentially expressed genes in co-cultured adipocytes treated with cortisol, ... Role of macrophage in TNF and associated signalling to induce adipogenesis in pre-adipocytes. Studies have associated inflamed ...
https://journals.biologists.com/bio/article/7/8/bio034629/2139/Role-of-murine-macrophage-in-temporal-regulation
FGF signaling promotes precursor spreading for adult adipogenesis in Drosophila - preLightsFGF signaling promotes precursor spreading for adult adipogenesis in Drosophila - preLights
The authors then turn to the role of FGF signalling in adult adipogenesis. They find expression of the FGF receptor heartless ( ... Thus, it provides strong evidence for de novo adipogenesis rather than for derivation of the adult fat body from persisting ... FGF signaling promotes precursor spreading for adult adipogenesis in Drosophila. Yuting Lei, Yuwei Huang, Ke Yang, Xueya Cao, ... does it come from the persisting larval cells or is there de novo adult adipogenesis? In this study, Lei et al., identify the ...
https://prelights.biologists.com/highlights/fgf-signaling-promotes-precursor-spreading-for-adult-adipogenesis-in-drosophila/
HOX-7 suppresses body weight gain and adipogenesis-related gene expression in high-fat-diet-induced obese mice | BMC...HOX-7 suppresses body weight gain and adipogenesis-related gene expression in high-fat-diet-induced obese mice | BMC...
From: HOX-7 suppresses body weight gain and adipogenesis-related gene expression in high-fat-diet-induced obese mice ...
https://bmccomplementmedtherapies.biomedcentral.com/articles/10.1186/1472-6882-14-505/tables/1
Fibrogenesis and Adipogenesis in Farm Animals and Rodents by Chaoyang Li"Fibrogenesis and Adipogenesis in Farm Animals and Rodents" by Chaoyang Li
Excessive adipogenesis along with fibrogenesis in the chicken breast, unlike in the beef steak, would exert an unpleasant ... we aimed to provide additional insight into the mechanisms of fibrogenesis and adipogenesis based on different animal models to ... Excessive adipogenesis along with fibrogenesis in the chicken breast, unlike in the beef steak, would exert an unpleasant ... In this dissertation, we aimed to provide additional insight into the mechanisms of fibrogenesis and adipogenesis based on ...
https://digitalcommons.lsu.edu/gradschool_dissertations/5747/
ETO/MTG8 is an inhibitor of C/EBP beta activity and a regulator of early adipogenesis<...ETO/MTG8 is an inhibitor of C/EBP beta activity and a regulator of early adipogenesis<...
ETO/MTG8 is an inhibitor of C/EBP beta activity and a regulator of early adipogenesis. / Rochford, JJ; Semple, RK; Laudes, M et ... ETO/MTG8 is an inhibitor of C/EBP beta activity and a regulator of early adipogenesis. Molecular and Cellular Biology. 2004 Nov ... ETO/MTG8 is an inhibitor of C/EBP beta activity and a regulator of early adipogenesis. In: Molecular and Cellular Biology. 2004 ... Dive into the research topics of ETO/MTG8 is an inhibitor of C/EBP beta activity and a regulator of early adipogenesis. ...
https://abdn.pure.elsevier.com/en/publications/etomtg8-is-an-inhibitor-of-cebp-beta-activity-and-a-regulator-of-
Adipogenesis | Pharmacognosy JournalAdipogenesis | Pharmacognosy Journal
Inhibitory Effect of Carallia Brachiata Extract Through Regulation of Adipogenesis Pathways in 3T3-L1 Cells. 1 month 1 week ago ...
https://phcogj.com/tags/adipogenesis
IMSEAR at SEARO: Cellular and molecular biology of adiposity and adipogenesis.
Cellular and molecular biology of adiposity and adipogenesis. Journal of the Association of Physicians of India. 2002 Oct; 50 ...
https://imsear.searo.who.int/handle/123456789/93141?locale=zh_TW
adipogenesis - ZooTerms (Dictionary of Invertebrate Zoology)adipogenesis - ZooTerms (Dictionary of Invertebrate Zoology)
adipogenesis (noun; Latin adeps, fat; Greek gennaein, to produce): The formation of fat or fatty tissue. ... Retrieved from "https://species-id.net/zt/index.php?title=adipogenesis&oldid=79915" ...
https://species-id.net/zt/index.php?title=adipogenesis&printable=yes
Direct conversion of human umbilical cord mesenchymal stem cells into retinal pigment epithelial cells for treatment of retinal...Direct conversion of human umbilical cord mesenchymal stem cells into retinal pigment epithelial cells for treatment of retinal...
For adipogenesis, the cells were cultured in adipogenic induction medium (DMEM supplemented with 10% FBS, 10−7 M dexamethasone ... Induction of adipogenesis, osteogenesis, and chondrogenesis. hUCMSCs were induced to differentiate into adipocytes, osteoblasts ...
https://www.nature.com/articles/s41419-022-05199-5?error=cookies_not_supported&code=b11dc825-6928-452c-80f4-a99b44f581cb
Temporal expression pattern of Bardet-Biedl syndrome genes in adipogenesis<...Temporal expression pattern of Bardet-Biedl syndrome genes in adipogenesis<...
keywords = "3T3-F442A, Adipogenesis, Adipose tissue, Bardet-Biedl syndrome, Obesity",. author = "Efrat Forti and Olga Aksanov ... Forti, Efrat ; Aksanov, Olga ; Birk, Ruth Z. / Temporal expression pattern of Bardet-Biedl syndrome genes in adipogenesis. In: ... Forti, E., Aksanov, O., & Birk, R. Z. (2007). Temporal expression pattern of Bardet-Biedl syndrome genes in adipogenesis. ... To date, 11 BBS genes have been cloned (BBS1-BBS11). However, the function of BBS genes in adipogenesis is unknown. Moreover, ...
https://cris.bgu.ac.il/en/publications/temporal-expression-pattern-of-bardet-biedl-syndrome-genes-in-adi
497 THE ROLE OF FSH IN CASTRATION INDUCED ADIPOGENESIS. HIGHLIGHTING DIFFERENCES BETWEEN ORCHIECTOMY, GNRH AGONISTS AND...
497 THE ROLE OF FSH IN CASTRATION INDUCED ADIPOGENESIS. HIGHLIGHTING DIFFERENCES BETWEEN ORCHIECTOMY, GNRH AGONISTS AND ...
https://experts.mcmaster.ca/display/publication460568
High hydrostatic pressure extract of Siegesbeckia orientalis inhibits adipogenesis through the activation of the Wnt/β-catenin...High hydrostatic pressure extract of Siegesbeckia orientalis inhibits adipogenesis through the activation of the Wnt/β-catenin...
SPW-HHPE inhibited adipogenesis by reducing intracellular lipid accumulation. SPW-HHPE reduced the mRNA and protein expression ... SPW-HHPE inhibited adipogenesis by reducing intracellular lipid accumulation. SPW-HHPE reduced the mRNA and protein expression ... SPW-HHPE inhibited adipogenesis by reducing intracellular lipid accumulation. SPW-HHPE reduced the mRNA and protein expression ... SPW-HHPE inhibited adipogenesis by reducing intracellular lipid accumulation. SPW-HHPE reduced the mRNA and protein expression ...
https://yonsei.pure.elsevier.com/en/publications/high-hydrostatic-pressure-extract-of-siegesbeckia-orientalis-inhi
A circadian-regulated gene, Nocturnin, promotes adipogenesis by stimulating PPAR-gamma nuclear translocationA circadian-regulated gene, Nocturnin, promotes adipogenesis by stimulating PPAR-gamma nuclear translocation
EFFECT OF HYPEROSIDE ON THE INHIBITION OF ADIPOGENESIS IN 3T3-L1 ADIPOCYTES | AVESİSEFFECT OF HYPEROSIDE ON THE INHIBITION OF ADIPOGENESIS IN 3T3-L1 ADIPOCYTES | AVESİS
Keywords: adipogenesis, hyperoside, transcriptional factors, adipogenic genes, adipokines, 3T3-L1 adipocytes, PPAR-GAMMA, ... EFFECT OF HYPEROSIDE ON THE INHIBITION OF ADIPOGENESIS IN 3T3-L1 ADIPOCYTES ...
https://avesis.yyu.edu.tr/yayin/0f1fa330-0071-48f5-a180-05234f649b16/effect-of-hyperoside-on-the-inhibition-of-adipogenesis-in-3t3-l1-adipocytes
Bisecting GlcNAc protein N-glycosylation is characteristic of human adipogenesis - Projects - Macquarie UniversityBisecting GlcNAc protein N-glycosylation is characteristic of human adipogenesis - Projects - Macquarie University
Bisecting GlcNAc protein N-glycosylation is characteristic of human adipogenesis. Katherine Wongtrakul-Kish, Benjamin R. ...
https://researchers.mq.edu.au/en/publications/bisecting-glcnac-protein-n-glycosylation-is-characteristic-of-hum/projects/?status=RUNNING
Pyrocatechol, a component of coffee, suppresses LPS-induced inflammatory responses by inhibiting NF-κB and activating Nrf2 |...Pyrocatechol, a component of coffee, suppresses LPS-induced inflammatory responses by inhibiting NF-κB and activating Nrf2 |...
Coffee extract inhibits adipogenesis in 3T3-L1 preadipocyes by interrupting insulin signaling through the downregulation of ...
https://www.nature.com/articles/s41598-020-59380-x?error=cookies_not_supported&code=970d1222-7b9c-4f10-a5de-fa36a01afc50
The obesity susceptibility gene TMEM18 promotes adipogenesis through direct activation of PPARG signalling :: MPG.PuReThe obesity susceptibility gene TMEM18 promotes adipogenesis through direct activation of PPARG signalling :: MPG.PuRe
The obesity susceptibility gene TMEM18 promotes adipogenesis through direct activation of PPARG signalling ... 2019). The obesity susceptibility gene TMEM18 promotes adipogenesis through direct activation of PPARG signalling. Diabetologia ... The obesity susceptibility gene TMEM18 promotes adipogenesis through direct activation of PPARG signalling ...
https://pure.mpg.de/pubman/faces/ViewItemOverviewPage.jsp?itemId=item_3169892
Plus itPlus it
2. Adipogenesis. Synovial membrane-derived cells were seeded (2 × 105 cells/8.6 cm2) and cultured in a one-well chamber slide ... Adipogenesis was induced by culturing for 3 weeks using adipogenic differentiation medium (Cambrex). After fixation in 4% ... adipogenesis by using a stain for lipids, and osteogenesis by detecting calcium deposits. Coexpression of CD44, CD73, CD90, and ...
https://www.jrheum.org/content/38/2/339
Infantile Hemangioma Medication: Beta-adrenergic Blocker, Antiglaucoma, Beta-Blockers, Corticosteroids, Interferons, Biologic...Infantile Hemangioma Medication: Beta-adrenergic Blocker, Antiglaucoma, Beta-Blockers, Corticosteroids, Interferons, Biologic...
Mesenchymal stem cells and adipogenesis in hemangioma involution. Stem Cells. 2006 Jun. 24(6):1605-12. [QxMD MEDLINE Link]. ...
https://www.medscape.com/answers/1083849-100852/what-is-the-role-of-becaplermin-in-the-treatment-of-infantile-hemangiomas
Adenovirus 36 DNA in Adipose Tissue of Patient with Unusual Visceral Obesity - Volume 16, Number 5-May 2010 - Emerging...
Rogers PM, Fusinski KA, Rathod MA, Loiler SA, Pasarica M, Shaw MK, Human adenovirus Ad-36 induces adipogenesis via its E4 orf-1 ... Adv 36 causes adipogenesis in animals and humans. Development of massive lipomatosis may be caused by Adv 36. ...
https://wwwnc.cdc.gov/eid/article/16/5/09-1271
Infantile Hemangioma Clinical Presentation: History, Physical Examination, ComplicationsInfantile Hemangioma Clinical Presentation: History, Physical Examination, Complications
Mesenchymal stem cells and adipogenesis in hemangioma involution. Stem Cells. 2006 Jun. 24(6):1605-12. [QxMD MEDLINE Link]. ...
http://emedicine.medscape.com/article/1083849-clinical
A metabolite of daidzein, 6,7,4-trihydroxyisoflavone, suppresses adipogenesis in 3T3-L1 preadipocytes via ATP-competitive...A metabolite of daidzein, 6,7,4'-trihydroxyisoflavone, suppresses adipogenesis in 3T3-L1 preadipocytes via ATP-competitive...
... suppresses adipogenesis in 3T3-L1 preadipocytes via ATP-competitive inhibition of PI3K. ... 3T3-L1 Cells, Adenosine Triphosphate, Adipogenesis, Animals, Binding, Competitive, CCAAT-Enhancer-Binding Protein-alpha, Cell ... Our objective was to determine the effects of 6,7,4-THIF on adipogenesis in 3T3-L1 preadipocytes and elucidate the mechanisms ... METHODS AND RESULTS: Adipogenesis was stimulated in 3T3-L1 preadipocytes. Both 6,7,4-THIF and daidzein were treated in the ...
https://fwcs.oregonstate.edu/biblio/metabolite-daidzein-674-trihydroxyisoflavone-suppresses-adipogenesis-3t3-l1-preadipocytes-atp
MetabolismMetabolism
The mitophagy receptor Bcl-2-like protein 13 stimulates adipogenesis by regulating mitochondrial oxidative phosphorylation and ...
https://www.jbc.org/Metabolism?startPage=&KeywordRaw=energy%20metabolism
Interleukin-11 Receptor Subunit Alpha-1 is Required for Maximal Airway Responsiveness to Methacholine After Acute Exposure to...Interleukin-11 Receptor Subunit Alpha-1 is Required for Maximal Airway Responsiveness to Methacholine After Acute Exposure to...
Interleukin (IL)-11, a pleiotropic, cationic cytokine, contributes to numerous biological processes, including adipogenesis, ...
https://www.cdc.gov/niosh/data/datasets/rd-1047-2022-0/default.html
The Beneficial Effects of Quercetin, Curcumin, and Resveratrol in ObesityThe Beneficial Effects of Quercetin, Curcumin, and Resveratrol in Obesity
... inhibiting adipogenesis and lipogenesis, and suppressing the differentiation of preadipocytes to mature adipocytes. ... Resveratrol can inhibit adipogenesis by reducing the stability and transcriptional activity of PPARγ [78, 79] and prevent ... S. Rayalam, J. Y. Yang, S. Ambati, M. A. Della-Fera, and C. A. Baile, "Resveratrol induces apoptosis and inhibits adipogenesis ... Curcumin may have a significant effect on adipogenesis. In primary human adipocytes and murine 3T3-L1 adipocytes, curcumin ...
https://www.hindawi.com/journals/omcl/2017/1459497/
Brown adipose tissue and aging : Current Opinion in Clinical Nutrition & Metabolic CareBrown adipose tissue and aging : Current Opinion in Clinical Nutrition & Metabolic Care
adipogenesis , IDEAL-MRI , PET/computed tomography scan Search for Similar Articles. You may search for similar articles that ...
https://journals.lww.com/co-clinicalnutrition/Abstract/2011/01000/Brown_adipose_tissue_and_aging.2.aspx
Endoplasmic Reticulum Stress Regulates Adipocyte Resistin Expression | Diabetes | American Diabetes AssociationEndoplasmic Reticulum Stress Regulates Adipocyte Resistin Expression | Diabetes | American Diabetes Association
PPARγ, which is crucial for adipogenesis (34,35), also induces resistin expression during adipocyte differentiation (36). ... Stimulation of adipogenesis in fibroblasts by PPAR gamma 2, a lipid-activated transcription factor ... Sequential gene promoter interactions by C/EBPβ, C/EBPα, and PPARγ during adipogenesis ...
https://diabetesjournals.org/diabetes/article/58/8/1879/15142/Endoplasmic-Reticulum-Stress-Regulates-Adipocyte
Suppresses adipogenesisAdiposePromotesOsteogenesisRegulationInhibitory EffectAdipocytesAccumulationPreadipocyteMolecular biologyCellsMechanismsReceptorDifferentiationTemporalEarlyExpressionEnvironmentalAnimalsFunctionRoleMouseMesenchymal stemPromotes adipogenesisPreadipocytesRole in adipogenesisCapable of promoting adipogenesisHuman AdipogenesisInduces adipogenesisInhibit adipogenesisLipidEarly phase of adipogenesisMaster regulator of adipogenesisGenesInhibits adipogenesis viaProcess called adipogenesisPPARRegulatorPPARgFunction and adipogenesisAdipose tissueVivoInsulin ResistanceVitro adipogenesisControls adipogenesisMyogenesisInhibitionMRNAInduction2019InhibitorTranscriptionFibroblastsFetalOsteoblastogenesisDiet-induced obesityBonePathways
Suppresses adipogenesis3
  • These results suggest that SPW-HHPE suppresses adipogenesis by stimulating Wnt/β-catenin pathway. (elsevier.com)
  • A metabolite of daidzein, 6,7,4'-trihydroxyisoflavone, suppresses adipogenesis in 3T3-L1 preadipocytes via ATP-competitive inhibition of PI3K. (oregonstate.edu)
  • Our results suggest that 6,7,4'-THIF suppresses adipogenesis in 3T3-L1 preadipocytes by directly targeting PI3K. (oregonstate.edu)
Adipose2
  • These polyphenols may play beneficial effects on adipose tissue under obese condition by alleviating intracellular oxidative stress, reducing chronic low-grade inflammation, inhibiting adipogenesis and lipogenesis, and suppressing the differentiation of preadipocytes to mature adipocytes. (hindawi.com)
  • The aim of this thesis was to develop an in vitro method for studying effects of environmental contaminants on adipogenesis in polar bear by using adipose tissue-derived stem cells derived from polar bear (pbASCs). (uib.no)
Promotes1
  • Environmental exposure to benzyl butyl phthalate promotes adipogenesis in the preadipocyte 3T3-L1. (cdc.gov)
Osteogenesis4
  • Based on MSC-derived bone formation assays both in vivo and in vitro, azoramide treatment displayed a cell fate determining ability in favor of adipogenesis over osteogenesis. (biomedcentral.com)
  • Moreover, both in vitro and in vivo studies have shown that a reciprocal fate decision is frequently observed between adipogenesis and osteogenesis. (biomedcentral.com)
  • Chondrogenesis was assessed by staining for proteoglycans and collagen type II, adipogenesis by using a stain for lipids, and osteogenesis by detecting calcium deposits. (jrheum.org)
  • The differentiation potentials of osteogenesis, chondrogenesis, and adipogenesis were evaluated with histological staining and quantitative polymerase chain reaction of differentiation marker genes. (researchsquare.com)
Regulation2
  • Regulation of adipogenesis, the root cause for obesity, is very poorly understood. (biologists.com)
  • EDCs may interact with nuclear receptors (NRs), including NRs involved in regulation of metabolism and adipogenesis, e.g. peroxisome proliferator- activated receptor gamma (PPARγ). (uib.no)
Inhibitory Effect1
  • The inhibitory effect of SPW-HHPE on adipogenesis was mainly attributed to the enhancement of the Wnt/β-catenin signaling pathway. (elsevier.com)
Adipocytes1
  • shRNA-mediated Thm1 knockdown in 3T3-L1 mouse pre-adipocytes reveals Thm1 deficiency increases adipogenesis and insulin sensitivity, providing an additional mechanism underlying Thm1-deficient obesity. (ku.edu)
Accumulation3
  • ETO prevents both the transcriptional activation of the C/EBPalpha promoter by C/EBPbeta and its concurrent accumulation in centromeric sites during early adipogenesis. (elsevier.com)
  • SPW-HHPE inhibited adipogenesis by reducing intracellular lipid accumulation. (elsevier.com)
  • 6,7,4'-THIF significantly suppressed MDI-induced lipid accumulation in the early stage of adipogenesis, attributable to a suppression of cell proliferation and the induction of cell cycle arrest. (oregonstate.edu)
Preadipocyte1
  • 3T3-F442A preadipocyte cells were harvested throughout the adipogenesis process (from day 1 to 8) at 1-day intervals. (bgu.ac.il)
Molecular biology1
  • IMSEAR at SEARO: Cellular and molecular biology of adiposity and adipogenesis. (who.int)
Cells1
  • However, the origins of the adult fat body are much less clear: does it come from the persisting larval cells or is there de novo adult adipogenesis? (biologists.com)
Mechanisms2
  • In this dissertation, we aimed to provide additional insight into the mechanisms of fibrogenesis and adipogenesis based on different animal models to help the discovery of new treatments in cardiovascular disease, beef breeding strategies, and feed nutrition modifications. (lsu.edu)
  • Our objective was to determine the effects of 6,7,4'-THIF on adipogenesis in 3T3-L1 preadipocytes and elucidate the mechanisms of action involved. (oregonstate.edu)
Receptor1
  • We observed that 6,7,4'-THIF, but not daidzein, inhibited MDI-induced adipogenesis significantly at 40 and 80 μM, associated with decreased peroxisome proliferator-activated receptor-γ and C/EBP-α protein expression. (oregonstate.edu)
Differentiation1
  • In this study, microcomputed tomography and histological analysis on bone morphogenetic protein (BMP)2-induced parietal periosteum bone formation assays, C3H10T1/2 and mouse bone marrow MSC-derived bone formation and adipogenesis assays, and specific staining for bone tissue and lipid droplets were used to evaluate the role of azoramide on the lineage determination of MSC differentiation. (biomedcentral.com)
Temporal1
  • These findings show for the first time a unique, temporal and synchronized expression of BBS genes during adipogenesis. (bgu.ac.il)
Early2
  • Here we show that ETO is highly expressed in preadipocytes and acts as an inhibitor of C/EBPbeta during early adipogenesis, contributing to its characteristically delayed activation. (elsevier.com)
  • High-mobility group box 2 protein is essential for the early phase of adipogenesis. (amedeo.com)
Expression2
  • ETO expression rapidly reduces after the initiation of adipogenesis, and this is essential to the normal induction of adipogenic gene expression. (elsevier.com)
  • The aim of our study was to investigate the expression of BBS genes throughout adipogenesis. (bgu.ac.il)
Environmental2
  • Interference by environmental contaminants on NRs regulating adipogenesis in polar bear may affect lipid metabolism and thereby decrease their ability to respond to climate change. (uib.no)
  • The use of pbASCs as an in vitro model for effects of environmental contaminants on adipogenesis can provide valuable information on how contaminant exposure can affect energy homeostasis in the polar bear. (uib.no)
Animals3
  • Adv 36 causes adipogenesis in animals and humans. (cdc.gov)
  • Adipogenesis in mice has alternating genetic requirements throughout the animals' lives. (the-scientist.com)
  • These changes are thought to be caused by the action causes adipogenesis in animals and humans. (cdc.gov)
Function3
  • Moreover, cAMP-protein kinase A (PKA)-mediated nuclear β-catenin activity was responsible for the negative function of azoramide on bone formation in favor of adipogenesis. (biomedcentral.com)
  • However, the function of BBS genes in adipogenesis is unknown. (bgu.ac.il)
  • BET bromodomain proteins regulate enhancer function during adipogenesis. (bvsalud.org)
Role1
  • The authors then turn to the role of FGF signalling in adult adipogenesis. (biologists.com)
Mouse1
  • In addition, loss of β-catenin in the mesenchyme of the developing mouse uterus was found to be a switch to adipogenesis in the myometrium [ 6 ]. (biomedcentral.com)
Mesenchymal stem2
  • Taken together, our study demonstrates that SCARA5 is a positive regulator in adipocyte lineage commitment and early adipogenesis in mesenchymal stem cells. (yonsei.ac.kr)
  • GNPDA2 Gene Affects Adipogenesis and Alters the Transcriptome Profile of Human Adipose-Derived Mesenchymal Stem Cells. (cdc.gov)
Promotes adipogenesis4
  • Vkorc1l1 promotes adipogenesis and possibly obesity. (nih.gov)
  • 7. Hydrogen sulfide promotes adipogenesis in 3T3L1 cells. (nih.gov)
  • The obesity-susceptibility gene TMEM18 promotes adipogenesis through activation of PPARG. (mpg.de)
  • Environmental exposure to benzyl butyl phthalate promotes adipogenesis in the preadipocyte 3T3-L1. (cdc.gov)
Preadipocytes7
  • Adipogenesis is induced by treating confluent preadipocytes with the adipogenic cocktail, which activates transcription factors (TFs) glucocorticoid receptor (GR) and CREB within minutes and increases expression of TFs C/EBPβ, C/EBPδ, KLF4, and Krox20 within hours. (nih.gov)
  • However, it has remained unclear whether endogenous KLF4 and Krox20 are required for adipogenesis in culture and in vivo Using conditional knockout mice and derived white and brown preadipocytes, we show that endogenous KLF4 and Krox20 are dispensable for adipogenesis in culture and for brown adipose tissue development in mice. (nih.gov)
  • When ectopically expressed in preadipocytes, each of these TFs is capable of promoting adipogenesis in culture. (nih.gov)
  • Using conditional knockout mice and derived preadipocytes, we demonstrate that endogenous GR is largely dispensable for adipogenesis in culture and brown adipose tissue development in mice. (nih.gov)
  • We hypothesized that exposing preadipocytes to TBBPA could influence adipogenesis via genes other than those in the PPARγ pathway due to its structural similarity to bisphenol A, which demonstrates varied endocrine disrupting activities. (nih.gov)
  • Using embryonic stem cells and 3T3-L1 preadipocytes, we show that calreticulin modulates adipogenesis. (rupress.org)
  • Wnt10b, an endogenous inhibitor of adipogenesis, maintains preadipocytes within an undifferentiated condition by suppressing adipogenic transcription elements. (lifescienceexec.com)
Role in adipogenesis2
  • These data suggest stereospecificity of CLA isomers and a possible regulatory role in adipogenesis. (nih.gov)
  • [ 16 ] Potential mechanisms through which TCF7L2 variants influence T2DM include its role in adipogenesis, myogenesis, and pancreatic islet development, as well as in beta-cell survival and insulin secretory granule function. (medscape.com)
Capable of promoting adipogenesis1
  • All of these TFs have been shown to be capable of promoting adipogenesis in culture when they are overexpressed. (nih.gov)
Human Adipogenesis4
  • A novel selective 11beta-hydroxysteroid dehydrogenase type 1 inhibitor prevents human adipogenesis. (ox.ac.uk)
  • The increase in 11beta-HSD1 mRNA expression and activity is essential for the induction of human adipogenesis. (ox.ac.uk)
  • Our previous data, illustrating decreased HA production during human adipogenesis, suggested an inhibitory role. (cardiff.ac.uk)
  • In human subjects, circulating HA correlated negatively with BMI and triglycerides (r = −0.396 (p = 0.002), r = −0.269 (p = 0.038), respectively), confirming an inhibitory role of HA in human adipogenesis. (cardiff.ac.uk)
Induces adipogenesis1
  • Peroxisome proliferator-activated receptor-gamma activation by thiazolidinediones induces adipogenesis in bone marrow stromal cells. (aspetjournals.org)
Inhibit adipogenesis1
  • AD did not inhibit adipogenesis at 10 μM concentration and also did not inhibitmyogenesis at 10 μM concentration. (scirp.org)
Lipid1
  • Placental DNA methylation was measured for 12 target candidate genes that are related to fetal growth, adipogenesis, lipid and energy metabolism, or long interspersed nuclear elements. (elsevier.com)
Early phase of adipogenesis2
  • These results challenge the existing model on transcriptional regulation in the early phase of adipogenesis and highlight the need of studying adipogenesis in vivo. (nih.gov)
  • We also show that while endogenous Krox20 and KLF4 are transiently induced in the early phase of adipogenesis in culture, they are dispensable for adipogenesis in vitro and in vivo. (nih.gov)
Master regulator of adipogenesis3
  • High throughput screening and binding assays have identified TBBPA as an agonist for human peroxisome proliferator-activated receptor gamma (PPARγ), the master regulator of adipogenesis. (nih.gov)
  • MED12 knockdown has produced similar results, but a potential interaction between MED12 and the master regulator of adipogenesis, PPARG, has been revealed through the application of delayed knockdown assays. (latech.edu)
  • PPARγ is a ligand-activated transcription factor (TF) and a master regulator of adipogenesis. (nih.gov)
Genes2
  • RNA sequencing analysis revealed decreased enrichment of genes associated with adipogenesis in inguinal white adipose of FMRKO mice. (endocrine.org)
  • H3K9 methyltransferase G9a represses PPARγ expression and adipogenesis ( EMBO J 2013 ) while H3K27 methyltransferase Ezh2 represses Wnt genes to facilitate adipogenesis ( PNAS 2010 ). (nih.gov)
Inhibits adipogenesis via1
  • Calreticulin inhibits adipogenesis via a negative feedback mechanism whereby the expression of calreticulin is initially up-regulated by peroxisome proliferator-activated receptor γ (PPARγ). (rupress.org)
Process called adipogenesis2
  • When hair growth begins, the fat layer expands in a process called adipogenesis. (nextbigfuture.com)
  • FKBP51 expression in adipose tissue increases with the formation of new fat cells, a process called adipogenesis. (uky.edu)
PPAR6
  • Additionally, we observed that DIM treatment (40 and 60 μM), but not I3C treatment, significantly inhibited MDI-induced adipogenesis by reducing the levels of several adipogenic proteins such as PPAR-γ and C/EBPα. (nih.gov)
  • MDI: adipogenesis positive control, ROSI: rosiglitazone and MDI (PPARγ agonist positive control). (nih.gov)
  • H3K4 methyltransferases MLL3/4 and associated PTIP directly control the induction of principal adipogenic TFs PPARγ and C/EBPα and are essential for adipogenesis ( Cell Metab 2009 , eLife 2013 ). (nih.gov)
  • PPARγ: a circadian transcription factor in adipogenesis and osteogenesis. (nih.gov)
  • Activation of transcription factor PPARγ plays an essential role in the adipogenesis. (scielo.org)
  • Certain fatty acids are PPARγ ligands and can control adipogenesis. (scielo.org)
Regulator1
  • 2013). Rap1 is a major telomere binding protein that also acts as a transcriptional regulator controlling adipogenesis. (mskcc.org)
PPARg2
  • While PPARg and C/EBPa/b/d have been shown to be critical for adipogenesis in mice, it has remained unclear whether endogenous GR, KLF4 and Krox20 are also required for adipogenesis in vivo. (nih.gov)
  • The induction of PPARg is required for adipogenesis, and this is regulated by a coordination of hormonal, epigenomic, and transcriptional factors that define a novel early stage of adipognesis. (nih.gov)
Function and adipogenesis2
  • CRISPR editing of the PPARGC1A Gly482ser (rs8192678) polymorphism in human white adipose cells shows differential effects on mitochondrial function and adipogenesis. (lu.se)
  • MYH10 governs adipocyte function and adipogenesis through its interaction with GLUT4. (tau.ac.il)
Adipose tissue4
  • Obesity is caused by an increase in adipose tissue mass, which results from the multiplication of fat cells through adipogenesis and the increased deposition of cytoplasmic triglycerides [ 2 ]. (springer.com)
  • Epigenomic mechanisms play critical roles in adipose tissue development (adipogenesis, reviewed in MCB 2019 ) and expansion. (nih.gov)
  • 2020) Sex differences in human adipose tissue gene expression and genetic regulation involve adipogenesis. (news-medical.net)
  • 3D Adipose Tissue Culture Links the Organotypic Microenvironment to Improved Adipogenesis. (ki.se)
Vivo3
  • Thus, the in-vitro results of AD on adipogenesis correlated with the in-vivo results of AD on fat-mass from clinical trials and suggested a possible difference in biological action between weak androgens (AD, DHEA) and strong androgens (T, DHT) on adipogenesis. (scirp.org)
  • Core fucosylation was also significantly increased during in vivo adipogenesis but did not correlate with an increase in Fut8 gene transcript. (edu.au)
  • Here, we hypothesized that supplementation with the insulin inhibitor and mitochondrial uncoupler, Tyrphostin (T-AG17), in vitro and in vivo inhibits adipogenesis and adipocyte hypertrophy. (uanl.mx)
Insulin Resistance2
  • Further studies are needed to delineate the underlying regulation of maximal triglyceride storage capacity, adipogenesis, and mediators of insulin resistance, and the unique biological properties of the small, as compared with large, adipose cells. (grantome.com)
  • Hypoxia affects several biological functions including adipogenesis, inflammation, insulin resistance and metabolism affecting the overall degree of adiposity. (endocrine-abstracts.org)
Vitro adipogenesis1
  • Here, we report upregulated Mecp2 in white adipose tissues (WAT) of obese humans, as well as in obese mice and during in vitro adipogenesis. (diabetesjournals.org)
Controls adipogenesis1
  • Cathepsin L activity controls adipogenesis and glucose tolerance. (harvard.edu)
Myogenesis2
  • Addition of bicalutamide, an androgen receptor (AR) antagonist decreased myogenesis and increased adipogenesis, indicating that the effect of AD was mediated through AR. (scirp.org)
  • The epigenomic reader Brd4 binds to active enhancers to control cell identity gene induction in adipogenesis and myogenesis. (nih.gov)
Inhibition1
  • Xanthohumol also regulates various metabolic processes including the inhibition of triglyceride formation, atherosclerotic plaque and adipogenesis. (sigmaaldrich.com)
MRNA1
  • In addition, fat depots responded differently to estrogen withdrawal (e.g., selective mRNA enhancement of adipogenesis markers in subcutaneous and of inflammation in visceral fat pads) and replacement challenges. (inserm.fr)
Induction2
  • MLL3/4-associated PAGR1 regulates adipogenesis by controlling induction of C/EBPβ and C/EBPδ ( MCB 2020 ). (nih.gov)
  • Brd4 binding on active enhancers controls cell identity gene induction in adipogenesis ( Nat Commun 2017 ). (nih.gov)
20191
  • 2019. Cell shape alteration during adipogenesis is associated with coordinated matrix cues. (tau.ac.il)
Inhibitor1
  • Blocking adipogenesis with a novel and specific 11beta-HSD1 inhibitor may represent a novel approach to treat obesity in patients with MS. (ox.ac.uk)
Transcription2
  • It also decreased adipogenesis-related transcription factor expression, including peroxisome proliferator-activated receptor and CCAAT/enhancer-binding protein. (hindawi.com)
  • The transcription factor SOX6 contributes to the developmental origins of obesity by promoting adipogenesis. (gusto.sg)
Fibroblasts1
  • They observed reactive adipogenesis in some types of fibroblasts. (nih.gov)
Fetal2
  • We have shown that maternal obesity increases fetal intramuscular adipogenesis at mid-gestation. (uthscsa.edu)
  • Overexpression of let-7g decreased expression of inflammatory cytokines.Conclusion:Fetal muscle miRNA expression was altered due to maternal obesity, and let-7g downregulation may enhance intramuscular adipogenesis during fetal muscle development in the setting of maternal obesity. (uthscsa.edu)
Osteoblastogenesis1
  • A chromosomal inversion within a quantitative trait locus has a major effect on adipogenesis and osteoblastogenesis. (jax.org)
Diet-induced obesity1
  • We evaluated the safety of T-AG17 and its effects on physiological and molecular metabolic parameters including hormonal profile, glucose levels, adipogenesis and adipocyte hypertrophy in a diet-induced obesity model using C57BL/6 mice. (uanl.mx)
Bone1
  • However, the efficacy of the technique is controversial, and it has little effects on inhibiting bone marrow adipogenesis and fat cell hypertrophy [ 8 ]. (biomedcentral.com)
Pathways2
  • Although molecular pathways governing adipogenesis are well delineated, the structure of the nuclear lamina and nuclear-cytoskeleton junction in this process are not. (maastrichtuniversity.nl)
  • This study will describe the signaling pathways of adipogenesis and marbling by transcriptome sequencing of steers that received vitamin A during the neonatal phase as a strategy to increase IMF deposition. (fapesp.br)
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