Adhesins escherichia coli. Medical search. Definitions

Uropathogenic Escherichia coli: Strains of Escherichia coli that preferentially grow and persist within the urinary tract. They exhibit certain virulence factors and strategies that cause urinary tract infections.Urinary Tract Infections: Inflammatory responses of the epithelium of the URINARY TRACT to microbial invasions. They are often bacterial infections with associated BACTERIURIA and PYURIA.Adhesins, Escherichia coli: Thin, filamentous protein structures, including proteinaceous capsular antigens (fimbrial antigens), that mediate adhesion of E. coli to surfaces and play a role in pathogenesis. They have a high affinity for various epithelial cells.Fimbriae, Bacterial: Thin, hairlike appendages, 1 to 20 microns in length and often occurring in large numbers, present on the cells of gram-negative bacteria, particularly Enterobacteriaceae and Neisseria. Unlike flagella, they do not possess motility, but being protein (pilin) in nature, they possess antigenic and hemagglutinating properties. They are of medical importance because some fimbriae mediate the attachment of bacteria to cells via adhesins (ADHESINS, BACTERIAL). Bacterial fimbriae refer to common pili, to be distinguished from the preferred use of "pili", which is confined to sex pili (PILI, SEX).Escherichia coli Infections: Infections with bacteria of the species ESCHERICHIA COLI.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Urinary Tract: The duct which coveys URINE from the pelvis of the KIDNEY through the URETERS, BLADDER, and URETHRA.Escherichia coli Proteins: Proteins obtained from ESCHERICHIA COLI.Bacterial Adhesion: Physicochemical property of fimbriated (FIMBRIAE, BACTERIAL) and non-fimbriated bacteria of attaching to cells, tissue, and nonbiological surfaces. It is a factor in bacterial colonization and pathogenicity.Fimbriae Proteins: Proteins that are structural components of bacterial fimbriae (FIMBRIAE, BACTERIAL) or sex pili (PILI, SEX).DNA Probes: Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.Adhesins, Bacterial: Cell-surface components or appendages of bacteria that facilitate adhesion (BACTERIAL ADHESION) to other cells or to inanimate surfaces. Most fimbriae (FIMBRIAE, BACTERIAL) of gram-negative bacteria function as adhesins, but in many cases it is a minor subunit protein at the tip of the fimbriae that is the actual adhesin. In gram-positive bacteria, a protein or polysaccharide surface layer serves as the specific adhesin. What is sometimes called polymeric adhesin (BIOFILMS) is distinct from protein adhesin.Diarrhea: An increased liquidity or decreased consistency of FECES, such as running stool. Fecal consistency is related to the ratio of water-holding capacity of insoluble solids to total water, rather than the amount of water present. Diarrhea is not hyperdefecation or increased fecal weight.Adhesins, Escherichia coli: Thin, filamentous protein structures, including proteinaceous capsular antigens (fimbrial antigens), that mediate adhesion of E. coli to surfaces and play a role in pathogenesis. They have a high affinity for various epithelial cells.Awards and Prizes Bacterial Adhesion: Physicochemical property of fimbriated (FIMBRIAE, BACTERIAL) and non-fimbriated bacteria of attaching to cells, tissue, and nonbiological surfaces. It is a factor in bacterial colonization and pathogenicity.Fimbriae Proteins: Proteins that are structural components of bacterial fimbriae (FIMBRIAE, BACTERIAL) or sex pili (PILI, SEX).Diarrhea, Infantile: DIARRHEA occurring in infants from newborn to 24-months old.Escherichia coli Infections: Infections with bacteria of the species ESCHERICHIA COLI.Fimbriae, Bacterial: Thin, hairlike appendages, 1 to 20 microns in length and often occurring in large numbers, present on the cells of gram-negative bacteria, particularly Enterobacteriaceae and Neisseria. Unlike flagella, they do not possess motility, but being protein (pilin) in nature, they possess antigenic and hemagglutinating properties. They are of medical importance because some fimbriae mediate the attachment of bacteria to cells via adhesins (ADHESINS, BACTERIAL). Bacterial fimbriae refer to common pili, to be distinguished from the preferred use of "pili", which is confined to sex pili (PILI, SEX).Shiga Toxin: A toxin produced by SHIGELLA DYSENTERIAE. It is the prototype of class of toxins that inhibit protein synthesis by blocking the interaction of ribosomal RNA; (RNA, RIBOSOMAL) with PEPTIDE ELONGATION FACTORS.Shiga-Toxigenic Escherichia coli: Strains of ESCHERICHIA COLI with the ability to produce at least one or more of at least two antigenically distinct, usually bacteriophage-mediated cytotoxins: SHIGA TOXIN 1 and SHIGA TOXIN 2. These bacteria can cause severe disease in humans including bloody DIARRHEA and HEMOLYTIC UREMIC SYNDROME.Shiga Toxins: A class of toxins that inhibit protein synthesis by blocking the interaction of ribosomal RNA; (RNA, RIBOSOMAL) with PEPTIDE ELONGATION FACTORS. They include SHIGA TOXIN which is produced by SHIGELLA DYSENTERIAE and a variety of shiga-like toxins that are produced by pathologic strains of ESCHERICHIA COLI such as ESCHERICHIA COLI O157.Escherichia coli Infections: Infections with bacteria of the species ESCHERICHIA COLI.Escherichia coli O157: A verocytotoxin-producing serogroup belonging to the O subfamily of Escherichia coli which has been shown to cause severe food-borne disease. A strain from this serogroup, serotype H7, which produces SHIGA TOXINS, has been linked to human disease outbreaks resulting from contamination of foods by E. coli O157 from bovine origin.Escherichia coli Proteins: Proteins obtained from ESCHERICHIA COLI.Adhesins, Bacterial: Cell-surface components or appendages of bacteria that facilitate adhesion (BACTERIAL ADHESION) to other cells or to inanimate surfaces. Most fimbriae (FIMBRIAE, BACTERIAL) of gram-negative bacteria function as adhesins, but in many cases it is a minor subunit protein at the tip of the fimbriae that is the actual adhesin. In gram-positive bacteria, a protein or polysaccharide surface layer serves as the specific adhesin. What is sometimes called polymeric adhesin (BIOFILMS) is distinct from protein adhesin.Shiga Toxin 2: A toxin produced by certain pathogenic strains of ESCHERICHIA COLI such as ESCHERICHIA COLI O157. It shares 50-60% homology with SHIGA TOXIN and SHIGA TOXIN 1.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Shiga Toxin 1: A toxin produced by certain pathogenic strains of ESCHERICHIA COLI such as ESCHERICHIA COLI O157. It is closely related to SHIGA TOXIN produced by SHIGELLA DYSENTERIAE.Food Microbiology: The presence of bacteria, viruses, and fungi in food and food products. This term is not restricted to pathogenic organisms: the presence of various non-pathogenic bacteria and fungi in cheeses and wines, for example, is included in this concept.Listeria monocytogenes: A species of gram-positive, rod-shaped bacteria widely distributed in nature. It has been isolated from sewage, soil, silage, and from feces of healthy animals and man. Infection with this bacterium leads to encephalitis, meningitis, endocarditis, and abortion.Foodborne Diseases: Acute illnesses, usually affecting the GASTROINTESTINAL TRACT, brought on by consuming contaminated food or beverages. Most of these diseases are infectious, caused by a variety of bacteria, viruses, or parasites that can be foodborne. Sometimes the diseases are caused by harmful toxins from the microbes or other chemicals present in the food. Especially in the latter case, the condition is often called food poisoning.Campylobacter jejuni: A species of bacteria that resemble small tightly coiled spirals. Its organisms are known to cause abortion in sheep and fever and enteritis in man and may be associated with enteric diseases of calves, lambs, and other animals.Escherichia coli O157: A verocytotoxin-producing serogroup belonging to the O subfamily of Escherichia coli which has been shown to cause severe food-borne disease. A strain from this serogroup, serotype H7, which produces SHIGA TOXINS, has been linked to human disease outbreaks resulting from contamination of foods by E. coli O157 from bovine origin.Cronobacter: A genus of gram-negative opportunistic foodborne pathogens.Cronobacter sakazakii: A species of gram-negative bacteria in the genus CHRONOBACTER, found in the environment and in foods.Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Truncated Hemoglobins: A family of hemoglobin-like proteins found in BACTERIA; PLANTS; and unicellular eukaryotes. Truncated hemoglobins are distantly related to vertebrate hemoglobins and are typically shorter than vertebrate hemoglobins by 20-40 residues.

(1/525) Molecular basis for the enterocyte tropism exhibited by Salmonella typhimurium type 1 fimbriae.

Salmonella typhimurium exhibits a distinct tropism for mouse enterocytes that is linked to their expression of type 1 fimbriae. The distinct binding traits of Salmonella type 1 fimbriae is also reflected in their binding to selected mannosylated proteins and in their ability to promote secondary bacterial aggregation on enterocyte surfaces. The determinant of binding in Salmonella type 1 fimbriae is a 35-kDa structurally distinct fimbrial subunit, FimHS, because inactivation of fimHS abolished binding activity in the resulting mutant without any apparent effect on fimbrial expression. Surprisingly, when expressed in the absence of other fimbrial components and as a translational fusion protein with MalE, FimHS failed to demonstrate any specific binding tropism and bound equally to all cells and mannosylated proteins tested. To determine if the binding specificity of Salmonella type 1 fimbriae was determined by the fimbrial shaft that is intimately associated with FimHS, we replaced the amino-terminal half of FimHS with the corresponding sequence from Escherichia coli FimH (FimHE) that contains the receptor binding domain of FimHE. The resulting hybrid fimbriae bearing FimHES on a Salmonella fimbrial shaft exhibited binding traits that resembled that of Salmonella rather than E. coli fimbriae. Apparently, the quaternary constraints imposed by the fimbrial shaft on the adhesin determine the distinct binding traits of S. typhimurium type 1 fimbriae. (+info)

(2/525) P fimbriae and other adhesins enhance intestinal persistence of Escherichia coli in early infancy.

Resident and transient Escherichia coli strains were identified in the rectal flora of 22 Pakistani infants followed from birth to 6 months of age. All strains were tested for O-antigen expression, adhesin specificity (P fimbriae, other mannose-resistant adhesins or type 1 fimbriae) and adherence to the colonic cell line HT-29. Resident strains displayed higher mannose-resistant adherence to HT-29 cells, and expressed P fimbriae (P = 0.0036) as well as other mannose-resistant adhesins (P = 0.012) more often than transient strains. In strains acquired during the first month of life, P fimbriae were 12 times more frequent in resident than in transient strains (P = 0.0006). The O-antigen distribution did not differ between resident and transient strains, and none of the resident P-fimbriated strains belonged to previously recognized uropathogenic clones. The results suggest that adhesins mediating adherence to intestinal epithelial cells, especially P fimbriae, enhance the persistence of E. coli in the large intestine of infants. (+info)

(3/525) Organization of biogenesis genes for aggregative adherence fimbria II defines a virulence gene cluster in enteroaggregative Escherichia coli.

Several virulence-related genes have been described for prototype enteroaggregative Escherichia coli (EAEC) strain 042, which has been shown to cause diarrhea in human volunteers. Among these factors are the enterotoxins Pet and EAST and the fimbrial antigen aggregative adherence fimbria II (AAF/II), all of which are encoded on the 65-MDa virulence plasmid pAA2. Using nucleotide sequence analysis and insertional mutagenesis, we have found that the genes required for the expression of each of these factors, as well as the transcriptional activator of fimbrial expression AggR, map to a distinct cluster on the pAA2 plasmid map. The cluster is 23 kb in length and includes two regions required for expression of the AAF/II fimbria. These fimbrial biogenesis genes feature a unique organization in which the chaperone, subunit, and transcriptional activator lie in one cluster, whereas the second, unlinked cluster comprises a silent chaperone gene, usher, and invasin reminiscent of Dr family fimbrial clusters. This plasmid-borne virulence locus may represent an important set of virulence determinants in EAEC strains. (+info)

(4/525) Adhesins as targets for vaccine development.

Blocking the primary stages of infection, namely bacterial attachment to host cell receptors and colonization of the mucosal surface, may be the most effective strategy to prevent bacterial infections. Bacterial attachment usually involves an interaction between a bacterial surface protein called an adhesin and the host cell receptor. Recent preclinical vaccine studies with the FimH adhesin (derived from uropathogenic Escherichia coli) have confirmed that antibodies elicited against an adhesin can impede colonization, block infection, and prevent disease. The studies indicate that prophylactic vaccination with adhesins can block bacterial infections. With recent advances in the identification, characterization, and isolation of other adhesins, similar approaches are being explored to prevent infections, from otitis media and dental caries to pneumonia and sepsis. (+info)

(5/525) The mast cell tumor necrosis factor alpha response to FimH-expressing Escherichia coli is mediated by the glycosylphosphatidylinositol-anchored molecule CD48.

Mast cells are well known for their harmful role in IgE-mediated hypersensitivity reactions, but their physiological role remains a mystery. Several recent studies have reported that mast cells play a critical role in innate immunity in mice by releasing tumor necrosis factor alpha (TNF-alpha) to recruit neutrophils to sites of enterobacterial infection. In some cases, the mast cell TNF-alpha response was triggered when these cells directly bound FimH on the surface of Escherichia coli. We have identified CD48, a glycosylphosphatidylinositol-anchored molecule, to be the complementary FimH-binding moiety in rodent mast cell membrane fractions. We showed that (i) pretreatment of mast cell membranes with antibodies to CD48 or phospholipase C inhibited binding of FimH+ E. coli, (ii) FimH+ E. coli but not a FimH- derivative bound isolated CD48 in a mannose-inhibitable manner, (iii) binding of FimH+ bacteria to Chinese hamster ovary (CHO) cells was markedly increased when these cells were transfected with CD48 cDNA, and (iv) antibodies to CD48 specifically blocked the mast cell TNF-alpha response to FimH+ E. coli. Thus, CD48 is a functionally relevant microbial receptor on mast cells that plays a role in triggering inflammation. (+info)

(6/525) Decay-accelerating factor and cytoskeleton redistribution pattern in HeLa cells infected with recombinant Escherichia coli strains expressing Dr family of adhesins.

Escherichia coli strains expressing Dr fimbriae are able to enter epithelial cells by interacting with a complement-regulatory protein, decay-accelerating factor. This model of bacterial internalization, with a well-characterized bacterial ligand and host receptor, provides a unique opportunity to investigate the early stages of invasion. We used immunofluorescence staining techniques to examine the distribution of receptor and cytoskeletal proteins in HeLa cells infected with E. coli recombinant strains that expressed Dr family of adhesins: Dr, Dr-II, F1845, AFA-I, and AFA-III. A major rearrangement of decay-accelerating factor was found at the adherence sites of recombinant strains expressing Dr, Dr-II, and F1845 adhesins. The changes in the distribution of receptor were significantly smaller on HeLa cells infected with E. coli bearing AFA-I or AFA-III afimbrial adhesins. Receptor aggregation was associated with the redistribution of cytoskeleton-associated proteins such as actin, alpha-actinin, ezrin, and occasionally tropomyosin. Purified Dr fimbriae coated on polystyrene beads were capable of triggering clustering of receptor and accumulating actin at the adhesion sites of beads to HeLa cells. Using scanning and transmission electron microscopic techniques, we have shown that beads coated with Dr fimbriae, as opposed to beads coated with bovine serum albumin, were enwrapped by cellular microvilli and ultimately internalized into HeLa cells. This indicates that interaction of Dr fimbriae with decay-accelerating factor is associated with redistribution of receptor and is sufficient to promote bacterial internalization. (+info)

(7/525) X-ray structure of the FimC-FimH chaperone-adhesin complex from uropathogenic Escherichia coli.

Type 1 pili-adhesive fibers expressed in most members of the Enterobacteriaceae family-mediate binding to mannose receptors on host cells through the FimH adhesin. Pilus biogenesis proceeds by way of the chaperone/usher pathway. The x-ray structure of the FimC-FimH chaperone-adhesin complex from uropathogenic Escherichia coli at 2.5 angstrom resolution reveals the basis for carbohydrate recognition and for pilus assembly. The carboxyl-terminal pilin domain of FimH has an immunoglobulin-like fold, except that the seventh strand is missing, leaving part of the hydrophobic core exposed. A donor strand complementation mechanism in which the chaperone donates a strand to complete the pilin domain explains the basis for both chaperone function and pilus biogenesis. (+info)

(8/525) Virulence characteristics of Escherichia coli in acute bacterial prostatitis.

To assess the urovirulence characteristics of Escherichia coli strains causing acute prostatitis, urinary isolates from men with acute prostatitis (n=107) and from women with acute uncomplicated pyelonephritis (n=76) were examined for the prevalence of sfa, foc, and 3 papG allele genotypes and phenotypes and for the production of alpha-hemolysin and cytotoxic necrotizing factor 1. The papG allele III and foc gene were found more frequently and the papG allele II less frequently among prostatitis than from pyelonephritis isolates. A higher proportion of hly+ cnf1+ genotype in prostatitis strains (64% vs. 36%) was particularly striking. Both prostatitis and pyelonephritis strains expressed virulence factors similarly except for a higher proportion of nonhemolytic prostatitis isolates. Although the pathogenetic mechanisms of urinary tract infections in men and women may differ, virulence factors such as adhesins and cytotoxins may have important roles in the pathogenesis of acute prostatitis. (+info)

Bacterial adhesin

The effectiveness of anti-adhesin antibodies is illustrated by studies with FimH, the adhesin of uropathogenic Escherichia coli ... Schembri MA, Klemm P (May 1998). "Heterobinary adhesins based on the Escherichia coli FimH fimbrial protein". Appl. Environ. ... August 1999). "X-ray structure of the FimC-FimH chaperone-adhesin complex from uropathogenic Escherichia coli". Science. 285 ( ... October 2011). "Type 1 fimbrial adhesin FimH elicits an immune response that enhances cell adhesion of Escherichia coli". ...

Trimeric autotransporter adhesin

2008). "UpaG, a new member of the trimeric autotransporter family of adhesins in uropathogenic Escherichia coli". J Bacteriol. ... YadA stands for Yersinia adhesin protein A. This protein domain is an example of Trimeric Autotransporter Adhesins, and it was ... "Oligomeric coiled-coil adhesin YadA is a double-edged sword". PLoS ONE. 5 (12): e15159. doi:10.1371/journal.pone.0015159. PMC ... Trimeric autotransporter adhesins have a unique structure. The structure they hold is crucial to their function. They all ...

Omptin

Found in the outer membrane of gram-negative enterobacteria such as Shigella flexneri, Yersinia pestis, Escherichia coli, and ... Kukkonen M, Korhonen TK (July 2004). "The omptin family of enterobacterial surface proteases/adhesins: from housekeeping in ... Escherichia coli to systemic spread of Yersinia pestis". Int. J. Med. Microbiol. 294 (1): 7-14. doi:10.1016/j.ijmm.2004.01.003 ...

List of MeSH codes (D12.776.097)

File "2006 MeSH Trees".) MeSH D12.776.097.120.050.040 -- adhesins, escherichia coli MeSH D12.776.097.120.300.500 -- transferrin ...

Jan Maree Tennent

The PapG adhesin of uropathogenic Escherichia coli contains separate regions for receptor binding and for the incorporation ... Tennent JM, Lindberg F, Normark S. Integrity of Escherichia coli P pili during biogenesis: properties and role of PapJ. Mol ... Her research into the genetic mechanism and control of host attachment by uropathogenic Escherichia coli was an important part ... Horizontal gene transfer of the Escherichia coli pap and prs pili operons as a mechanism for the development of tissue-specific ...

List of MeSH codes (D23)

File "2006 MeSH Trees".) MeSH D23.050.161.050 --- adhesins, bacterial MeSH D23.050.161.050.040 --- adhesins, escherichia coli ...

List of MeSH codes (D12.776.543)

File "2006 MeSH Trees".) MeSH D12.776.543.100.050.040 -- adhesins, escherichia coli MeSH D12.776.543.325.100.100 -- gtp-binding ...

Enteroinvasive Escherichia coli

EIEC are highly invasive, and they use adhesin proteins to bind to and enter intestinal cells. They produce no toxins, but ... Enterovirulent classes of E. coli are referred to as the EEC group (enterovirulent E. coli): Enteroinvasive E. coli (EIEC) ... "Shigella and enteroinvasive Escherichia coli strains are derived from distinct ancestral strains of E. coli". Microbiology. 144 ... Enteroinvasive Escherichia coli (EIEC) is a type of pathogenic bacteria whose infection causes a syndrome that is identical to ...

Pathogenic Escherichia coli

The Dr adhesins bind Dr blood group antigen (Dra) which is present on decay accelerating factor (DAF) on erythrocytes and other ... List of strains of Escherichia coli "Escherichia coli O157:H7". CDC Division of Bacterial and Mycotic Diseases. Retrieved 2011- ... "Escherichia coli in chronic inflammatory bowel diseases: An update on adherent invasive Escherichia coli pathogenicity". World ... Escherichia coli (/ˌɛʃəˈrɪkiə ˈkoʊlɪ/ Anglicized to /ˌɛʃəˈrɪkiə ˈkoʊlaɪ/; commonly abbreviated E. coli) is a gram-negative, rod ...

Enteroaggregative Escherichia coli

... typically associated with Shiga toxin-producing Escherichia coli, which often encode the adhesin intimin). The putative cause ... Enteroaggregative Escherichia coli (EAEC or EAggEC) are a pathotype of Escherichia coli associated with acute and chronic ... "Outbreak of Escherichia coli O104:H4 Infections Associated with Sprout Consumption - Europe and North America, May-July 2011". ... Ruan, Xiaosai; Crupper, Scott S.; Schultz, Bruce D.; Robertson, Donald C.; Zhang, Weiping (2012-08-15). "Escherichia coli ...

YadA bacterial adhesin protein domain

The Eib immunoglobulin-binding proteins from Escherichia coli were third, followed by the DsrA proteins of Haemophilus ducreyi ... and IgG Fc by distinct sequence segments of the EibF cell surface protein of Escherichia coli". Infect. Immun. 69 (12): 7293- ... YadA, an adhesin from Yersinia, was the first member of this family to be characterised. UspA2 from Moraxella was second. ... The importance of adhesins to YadA function and Yersinia survival is huge. Attachment further allows more interactions and ...

Staphylococcus saprophyticus

September 2012). "Effects of cranberry extracts on growth and biofilm production of Escherichia coli and Staphylococcus species ... The adhesin for S. saprophyticus is a lactosamine structure. S. saprophyticus produces no exotoxins. Patients with urinary ... after Escherichia coli. Sexual activity increases the risk of S. saprophyticus UTIs because bacteria are displaced from the ...

Virulence factor

Bacteria like Escherichia coli O157:H7 gain the majority of their virulence from mobile genetic elements. Gram-negative ... Bacteria produce various adhesins including lipoteichoic acid, trimeric autotransporter adhesins and a wide variety of other ... Exotoxins are also produced by a range of other bacteria including Escherichia coli; Vibrio cholerae (causative agent of ... These factors include adhesins, invasins, and antiphagocytic factors. The factors, including toxins, hemolysins, and proteases ...

Fim switch

The fim switch in Escherichia coli is the mechanism by which the fim gene cluster, encoding Type I Pili, is transcriptionally ... coding for an adhesin at the tip, to name just a few important elements. The fim S region is flanked by 9bp repeats that are ... Klemm, P (1986). "Two regulatory fim genes, fimB and fimE, control the phase variation of type 1 fimbriae in Escherichia coli ... These pili are virulence factors involved in adhesion, especially important in uropathogenic Escherichia coli. The gene ...

OmpA domain

OmpA from Escherichia coli is required for pathogenesis, and can interact with host receptor molecules. MotB (and MotA) serve ... De Mot R, Proost P, Van Damme J, Vanderleyden J (February 1992). "Homology of the root adhesin of Pseudomonas fluorescens OE ... Freudl R, Klose M, Henning U (June 1990). "Export and sorting of the Escherichia coli outer membrane protein OmpA". J. Bioenerg ... Hosking ER, Vogt C, Bakker EP, Manson MD (December 2006). "The Escherichia coli MotAB proton channel unplugged". J. Mol. Biol. ...

Bacterial display

Wang Y (2002). "The Function of OmpA in Escherichia coli". Biochem Biophys Res Commun. 292: 396-401. doi:10.1006/bbrc.2002.6657 ... Klemm P & Schembri MA (2000). "Bacterial Adhesins:Function and Structure". Int J Med Microbiol. 290: 27-35. doi:10.1016/S1438- ... "Cell Surface Exposure of the Outer Membrane Protein OmpA of Escherichia coli K-12". J Mol Biol. 188 (3): 491-4. doi:10.1016/ ... Many types of bacteria have cell surface proteins such as the enteropathogenic E. coli intimin protein which is involved in ...

Haemolysin expression modulating protein family

This family consists of haemolysin expression modulating protein (Hha) from Escherichia coli and its enterobacterial homologues ... YmoA modulates the expression of various virulence factors, such as Yop proteins and YadA adhesin, in response to temperature. ...

Protein tag

Bedouelle, Hugues; Duplay, Pascale (Feb 1988). "Production in Escherichia coli and one-step purification of bifunctional hybrid ... through engineering a bacterial adhesin". Proceedings of the National Academy of Sciences. 109 (12): E690-7. Bibcode:2012PNAS.. ... Solubilization tags are used, especially for recombinant proteins expressed in chaperone-deficient species such as E. coli, to ...

Dispersin B

... in Staphylococcus species and PGA in Escherichia coli. By degrading the biofilm matrix, Dispersin B allows for the release of ... Mack D, Fischer W, Krokotsch A, Leopold K, Hartmann R, Egge H, Laufs R (1996). "The intercellular adhesin involved in biofilm ... The three-dimensional structure of dispersin B was determined by expressing the protein in E. coli, purifying it from cultures ... and proteinaceous adhesins. It allows bacteria to adhere to host surfaces, protects the bacterial cells from host defenses, ...

Pathogenicity island

2001 Structural and sequence diversity of the pathogenicity island of uropathogenic Escherichia coli which encodes the USP ... Pathogenicity islands carry genes encoding one or more virulence factors, including, but not limited to, adhesins, secretion ... mosomal regions coding for fimbriae and hemolysins occur in vivo and in vitro in various extraintestinal Escherichia coli iso- ...

Chaperone-Usher fimbriae

2011). "Urinary tract infections of Escherichia coli strains of chaperone-usher system". Polish journal of microbiology. 60: ... Immune evasion is also facilitated by the tip adhesin, for example the binding of Decay accelerating factor (DAF) by gamma3 ... uropathogenic Escherichia coli) and Pseudomonas aeruginosa. All chaperone/usher systems are found within gene clusters ...

Bacterial small RNA

5 prime ureB sRNA Aar small RNA, an sRNA produced by species of Acinetobacter Bacillus subtilis BSR sRNAs Escherichia coli sRNA ... Outer membrane proteins (OMPs) include porins and adhesins. Numerous sRNAs regulate the expression of OMPs. The porins OmpC and ... MicF in E. coli was found to regulate the expression of a key structural gene that makes up the outer membrane of the E. coli ... Hershberg R, Altuvia S, Margalit H (April 2003). "A survey of small RNA-encoding genes in Escherichia coli". Nucleic Acids Res ...

Bacterial morphological plasticity

The exposure to β-lactam antibiotics induced the SOS response in Escherichia coli. During repair of DNA damage, the SOS ... with an increased number of adhesins participating in the interaction, making even harder the work for (PMN). The interaction ... This mechanism has been described in bacteria such as Escherichia coli and Helicobacter pylori. Oxidative stress, nutrient ... Justice, Sheryl S.; Hunstad (2006). "Filamentation by Escherichia coli subverts innate defenses during urinary tract infection ...

Secretion

The molecules secreted vary in size from the small Escherichia coli peptide colicin V, (10 kDa) to the Pseudomonas fluorescens ... Gerlach, R; Hensel, M (2007). "Protein secretion systems and adhesins: The molecular armory of Gram-negative pathogens". ... A common example of an autotransporter that uses this secretion system is the Trimeric Autotransporter Adhesins. Type VI ... "Structural Features of the Pseudomonas fluorescens Biofilm Adhesin LapA Required for LapG-Dependent Cleavage, Biofilm Formation ...

Sepsis

Other commonly implicated bacteria include Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa, and Klebsiella ... Bacterial virulence factors, such as glycocalyx and various adhesins, allow colonization, immune evasion, and establishment of ...

Gabriel Waksman

CU) pili have clear relevance in the pathogenicity of uropathogenic Escherichia coli, where CU pili mediate bacterial tropism ... coli adhesin to its human kidney receptor". Cell. 105 (6): 733-43. doi:10.1016/S0092-8674(01)00388-9. PMID 11440716. Sauer FG, ...

Transcriptional organization of the F1845 fimbrial adhesin determinant of Escherichia coli. - PubMed - NCBI

Transcriptional organization of the F1845 fimbrial adhesin determinant of Escherichia coli.. Bilge SS1, Apostol JM Jr, Fullner ... transcriptional organization of the gene cluster encoding the F1845 fimbrial adhesin of a diarrhoea-associated Escherichia coli ... were determined to share limited homology with the papB and papI genes of the P fimbrial adhesin, respectively. The 5 termini ...

https://www.ncbi.nlm.nih.gov/pubmed/8097864?dopt=Abstract

Phenotypical characterization and adhesin identification in Escherichia coli strains isolated from dogs with urinary tract...

Phenotypical characterization and adhesin identification in Escherichia coli strains isolated from dogs with urinary tract ... Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic Escherichia coli (EPEC) from calves and lambs with diarrhoea ... Escherichia coli is the most common pathogen associated with urinary tract infection (UTI) (5, 10, 30). E. coli that are ... Escherichia coli mediated urinary tract infections: Are there distinct uropathogenic E. coli (UPEC) pathotypes? FEMS Microbiol ...

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100045&lng=pt&nrm=iso&tlng=en

Regulatory Interactions among adhesin gene systems of uropathogenic Escherichia coli

... Lindberg, Stina Umeå University, Faculty ... Uropathogenic Escherichia coli strain J96 carries multiple determinants for fimbrial adhesins. The regulatory protein PapB of P ... coli Abstract [en]. The main causative agent of human urinary tract infections is the uropathogenic Escherichia coli (UPEC) ... 1. Molecular analysis of transcription factors in uropathogenic E. coli adhesin operons. Open this publication in new window or ...

http://umu.diva-portal.org/smash/record.jsf?pid=diva2:202063

Globoside-specific adhesins of uropathogenic Escherichia coli are encoded by similar trans-complementable gene clusters. |...

Globoside-specific adhesins of uropathogenic Escherichia coli are encoded by similar trans-complementable gene clusters.. B ... Globoside-specific adhesins of uropathogenic Escherichia coli are encoded by similar trans-complementable gene clusters. ... Globoside-specific adhesins of uropathogenic Escherichia coli are encoded by similar trans-complementable gene clusters. ... Globoside-specific adhesins of uropathogenic Escherichia coli are encoded by similar trans-complementable gene clusters. ...

https://jb.asm.org/content/162/3/1293?ijkey=2144c7e3b34e0509bec888d422bab4fb7b05348e&keytype2=tf_ipsecsha

Fimbrial adhesins produced by atypical enteropathogenic Escherichia coli strains | Murdoch Children's Research Institute

Fimbrial adhesins produced by atypical enteropathogenic Escherichia coli strains. Applied and environmental microbiology (2011 ...

https://www.mcri.edu.au/publications/fimbrial-adhesins-produced-atypical-enteropathogenic-escherichia-coli-strains

JCI - Localization of a domain in the FimH adhesin of Escherichia coli type 1 fimbriae capable of receptor recognition and use...

Localization of a domain in the FimH adhesin of Escherichia coli type 1 fimbriae capable of receptor recognition and use of a ... The FimH subunit of type 1-fimbriated Escherichia coli has been implicated as an important determinant of bacterial adherence ... coli. Only the antibody directed at the putative binding region of FimH (anti- s-FimH1-25) significantly reduced E. coli ... coli to the bladder mucosa in situ and in vivo in an established mouse model of cystitis. We generated translational fusion ...

https://www.jci.org/articles/view/119623

Afa, a Diffuse Adherence Fibrillar Adhesin Associated with Enteropathogenic Escherichia coli | Infection and Immunity

Diffusely adhering Escherichia coli (DAEC) strains of fecal origin rarely express F1845 adhesin. Microbiol. Immunol. 43:167-170 ... Afa, a Diffuse Adherence Fibrillar Adhesin Associated with Enteropathogenic Escherichia coli Rogéria Keller, Juana G. Ordoñez, ... Afa, a Diffuse Adherence Fibrillar Adhesin Associated with Enteropathogenic Escherichia coli Rogéria Keller, Juana G. Ordoñez, ... Afa, a Diffuse Adherence Fibrillar Adhesin Associated with Enteropathogenic Escherichia coli Rogéria Keller, Juana G. Ordoñez, ...

https://iai.asm.org/content/70/5/2681

Detection of pap, sfa, afa, foc, and fim Adhesin-Encoding Operons in Uropathogenic Escherichia coli Isolates Collected From...

Detection of pap, sfa, afa, foc, and fim Adhesin-Encoding Operons in Uropathogenic Escherichia coli Isolates Collected From ... Detection of pap, sfa, afa, foc, and fim Adhesin-Encoding Operons in Uropathogenic Escherichia coli Isolates Collected From ... Uropathogenic Escherichia coli (UPEC) with its virulence factors is the most prevalent cause of urinary tract infection (UTI). ... Adhesion Proteins-Encoding Operons; Urinary Tract Infections; Uropathogenic Escherichia coli; Virulence Genes ...

https://www.ncbi.nlm.nih.gov/pubmed/26464770

9 DNA PROBES FOR DETECTION OF TOXIN AND ADHESIN GENES IN ESCHERICHIA-COLI ISOLATED FROM DIARRHEAL DISEASE IN ANIMALS - Surrey...

WOODWARD, MJ and WRAY, C (1990) 9 DNA PROBES FOR DETECTION OF TOXIN AND ADHESIN GENES IN ESCHERICHIA-COLI ISOLATED FROM ... 9 DNA PROBES FOR DETECTION OF TOXIN AND ADHESIN GENES IN ESCHERICHIA-COLI ISOLATED FROM DIARRHEAL DISEASE IN ANIMALS ...

http://epubs.surrey.ac.uk/824427/

The TibA Adhesin/Invasin from Enterotoxigenic Escherichia coli Is Self Recognizing and Induces Bacterial Aggregation and...

The TibA Adhesin/Invasin from Enterotoxigenic Escherichia coli Is Self Recognizing and Induces Bacterial Aggregation and ... The TibA Adhesin/Invasin from Enterotoxigenic Escherichia coli Is Self Recognizing and Induces Bacterial Aggregation and ... The TibA Adhesin/Invasin from Enterotoxigenic Escherichia coli Is Self Recognizing and Induces Bacterial Aggregation and ... The TibA Adhesin/Invasin from Enterotoxigenic Escherichia coli Is Self Recognizing and Induces Bacterial Aggregation and ...

https://iai.asm.org/content/73/4/1954/article-info

UpaG, a new member of the trimeric autotransporter family of adhesins in uropathogenic Escherichia coli

... ... The ability of Escherichia coli to colonize both intestinal and extraintestinal sites is driven by the presence of specific ... In this study, we characterized a new trimeric AT adhesin (UpaG) from uropathogenic E. coli (UPEC). Molecular analysis of UpaG ... Thus, UpaG is a novel trimeric AT adhesin from E. coli that mediates aggregation, biofilm formation, and adhesion to various ...

https://research-repository.griffith.edu.au/handle/10072/25865

The sweet connection: Solving the riddle of multiple sugar-binding fimbrial adhesins in Escherichia coli: Multiple E. coli...

CU fimbriae homologues in the genome of commensal and pathogenic Escherichia coli and closely related bacteria. We propose that ... Among them, chaperone-usher (CU) fimbriae adhesins, related to prototypical type 1 fimbriae, interact in highly specific ways ... The sweet connection: Solving the riddle of multiple sugar-binding fimbrial adhesins in Escherichia coli: Multiple E. coli ... Solving the riddle of multiple sugar-binding fimbrial adhesins in Escherichia coli: Multiple E. coli fimbriae form a versatile ...

https://www.semanticscholar.org/paper/The-sweet-connection%3A-Solving-the-riddle-of-in-E.-a-Korea-Ghigo/ab3c05709df23efc82115ce04fa2f6c4b8fa2078

The pgaABCD Locus of Escherichia coli Promotes the Synthesis of a Polysaccharide Adhesin Required for Biofilm Formation |...

The pgaABCD Locus of Escherichia coli Promotes the Synthesis of a Polysaccharide Adhesin Required for Biofilm Formation. Xin ... Previous studies of the regulation of Escherichia coli biofilm formation suggested the involvement of an unknown adhesin. We ... The pgaABCD Locus of Escherichia coli Promotes the Synthesis of a Polysaccharide Adhesin Required for Biofilm Formation ... The pgaABCD Locus of Escherichia coli Promotes the Synthesis of a Polysaccharide Adhesin Required for Biofilm Formation ...

https://jb.asm.org/content/186/9/2724?ijkey=c6cce6f21cd173eba15e38d6ab29f814de5dc705&keytype2=tf_ipsecsha

A novel putative extraintestinal pathogenic Escherichia coli fimbrial adhesin identified via Signature-tagged Mutagenesis -...

A novel putative extraintestinal pathogenic Escherichia coli fimbrial adhesin identified via Signature-tagged Mutagenesis (2010 ...

https://bib.vetmed.fu-berlin.de/pubdb/pub/1662/

Novel roles for the AIDA adhesin from diarrheagenic Escherichia coli: : Cell aggregation and biofilm formation - Danish...

Diarrhea-causing Escherichia coli strains are responsible for numerous cases of gastrointestinal disease and constitute a ... AIDA is a potent bacterial adhesin associated with some diarrheagenic E. coli strains. AIDA mediates bacterial attachment to a ... Additionally, AIDA expression dramatically enhances biofilm formation by E. coli on abiotic surfaces in How chambers. Type: ... Here, we show that AIDA possesses self-association characteristics and can mediate autoaggregation of E. coli cells. We ...

https://www.forskningsdatabasen.dk/en/catalog/2389402030

ChemIDplus - 139246-84-7 - Adhesin (Escherichia coli clone pPLHD2 gene F17-G protein moiety reduced) - Searchable synonyms,...

Escherichia coli clone pPLHD2 gene F17-G protein moiety reduced) - Searchable synonyms, formulas, resource links, and other ... Substance Name: Adhesin (Escherichia coli clone pPLHD2 gene F17-G protein moiety reduced). RN: 139246-84-7. ...

https://chem.nlm.nih.gov/chemidplus/rn/139246-84-7

Recombinant Escherichia coli Type 1 fimbrin D-mannose specific adhesin(fimH) - Cusabio

It is produced in E.coli. High purity. Good price. ... Purchase Recombinant Escherichia coli Type 1 fimbrin D-mannose ... Recombinant Escherichia coli Type 1 fimbrin D-mannose specific adhesin(fimH). Recombinant Escherichia coli Type 1 fimbrin D- ... E.coli. CSB-BP362349ENV. CSB-MP362349ENV. Recombinant Escherichia coli Type 1 fimbrin D-mannose specific adhesin(fimH). ... E.coli. CSB-EP362349ENV. Recombinant Escherichia coli Type 1 fimbrin D-mannose specific adhesin(fimH). ...

https://www.cusabio.com/Recombinant-Protein/Recombinant-Escherichia-coli-protein-FimH-fimH--12922177.html

Subtractive hybridization and identification of putative adhesins in a Shiga toxin-producing eae-negative Escherichia coli. -...

We recently showed that STEC strains isolated in Chile displayed a wide variety of adhesins; here we demonstrate that some of ... The eae-encoded protein intimin is the main adhesin implicated in intestinal colonization in vivo. ... coli. The DNA sequence of the STEC 472-1 psu-int region identified five open reading frames with homology to phage genes. We ... coli (EHEC) O157 : H7 prototype strain EDL933. This phenotype is associated with the presence of adherence factors different ...

https://www.semanticscholar.org/paper/Subtractive-hybridization-and-identification-of-pu-Vidal-Prado/63c6cbf823aaf988e3cdb68d9b4e8744360da6a7

Structural Sampling of Glycan Int... preview & related info | Mendeley

... evolutionarily adapted by Escherichia coli strains for the colonization of epithelial linings. Using glycan arrays of the ... coli (ETEC and of the FimH adhesin from uropathogenic E. coli. This has led to the discovery of a more specific receptor for ... Sampling of Glycan Interaction Profiles Reveals Mucosal Receptors for Fimbrial Adhesins of Enterotoxigenic Escherichia coli. * ... Sampling of Glycan Interaction Profiles Reveals Mucosal Receptors for Fimbrial Adhesins of Enterotoxigenic Escherichia coli. ...

https://www.mendeley.com/catalogue/structural-sampling-glycan-interaction-profiles-reveals-mucosal-receptors-fimbrial-adhesins-enteroto/

Adhesins of Escherichia c… - Göteborgs universitet

Adhesins of Escherichia coli associated with extra-intestinal pathogenicity confer binding to colonic epithelial cells.. ... Escherichia coli adhesins are virulence factors in intestinal and extra-intestinal infections, but their role in normal ... Adhesins, Escherichia coli, metabolism, Bacterial Adhesion, Bacterial Proteins, metabolism, Cells, Cultured, Colon, cytology, ... E. coli with S-fimbrial adhesins (Sfa I or Sfa II), P or type 1 fimbriae, adhered in a non-polarized manner, and in similar ...

https://www.gu.se/forskning/publikation?publicationId=171745

Uropathogenic Escherichia coli strain J96 carries multiple determinants for fimbrial adhesins. (diva-portal.org)
The main causative agent of human urinary tract infections is the uropathogenic Escherichia coli (UPEC) pathotype. (diva-portal.org)
Globoside-specific adhesins of uropathogenic Escherichia coli are encoded by similar trans-complementable gene clusters. (asm.org)
Uropathogenic Escherichia coli frequently express globoside-specific adhesins, shown to mediate binding to uroepithelial cells. (asm.org)
Detection of pap, sfa, afa, foc, and fim Adhesin-Encoding Operons in Uropathogenic Escherichia coli Isolates Collected From Patients With Urinary T. (nih.gov)
Uropathogenic Escherichia coli (UPEC) with its virulence factors is the most prevalent cause of urinary tract infection (UTI). (nih.gov)
Here we report the structure-function relationships of Antigen 43 (Ag43a), a prototypic self-associating AT protein from uropathogenic Escherichia coli . (pnas.org)
Thus, ec240 dysregulated several uropathogenic Escherichia coli (UPEC) virulence factors through different mechanisms and independent of its effects on type 1 pilus biogenesis and may have potential as an antivirulence compound. (asm.org)
The purpose of this study was to elucidate what virulence factors uropathogenic Escherichia coli (UPEC) use to modulate NLRP3 inflammasome activation and subsequent IL-1β release and the role of NLRP3 for UPEC colonization of bladder epithelial cells. (frontiersin.org)
The effectiveness of anti-adhesin antibodies is illustrated by studies with FimH, the adhesin of uropathogenic Escherichia coli (UPEC). (wikipedia.org)

The UPEC strains generally carry multiple determinants for fimbrial adhesins. (diva-portal.org)
E. coli that are associated with UTI are commonly named uropathogenic isolates (UPEC), although there is evidence that different pathotypes may be related to UTI (19). (scielo.br)
In this study, we characterized a new trimeric AT adhesin (UpaG) from uropathogenic E. coli (UPEC). (edu.au)
Urinary tract infection (UTI), the majority caused by uropathogenic E. coli (UPEC), is one of the most common human infections and 60% of all women are expected to report at least one episode of UTI during their lifetime. (frontiersin.org)

Localization of a domain in the FimH adhesin of Escherichia coli type 1 fimbriae capable of receptor recognition and use of a domain-specific antibody to confer protection against experimental urinary tract infection. (jci.org)
The FimH subunit of type 1-fimbriated Escherichia coli has been implicated as an important determinant of bacterial adherence and colonization of the urinary tract. (jci.org)
Here, we sought to localize the functionally important domain(s) within the FimH molecule and to determine if antibodies against this domain would block adherence of type 1-fimbriated E. coli to the bladder mucosa in situ and in vivo in an established mouse model of cystitis. (jci.org)
Next, we compared the ability of mice passively immunized intraperitoneally with antisera raised against residues 1-25 and 253-264 of FimH or 1-13 of FimA to resist bladder colonization in vivo after intravesicular challenge with type 1-fimbriated E. coli. (jci.org)
Only the antibody directed at the putative binding region of FimH (anti- s-FimH1-25) significantly reduced E. coli bladder infections in the experimental mouse model of urinary tract infections. (jci.org)
Here we report the crystal structure of the full-length FimD usher bound to the FimC-FimH chaperone-adhesin complex and that of the unbound form of the FimD translocation domain. (nature.com)
FimH adhesin of type 1 pili is assembled into a fibrillar tip structure in the Enterobacteriaceae. (nature.com)
The best characterized bacterial adhesin is the type 1 fimbrial FimH adhesin. (wikipedia.org)

By restriction enzyme mapping, DNA hybridization, DNA sequencing, and protein expression in minicells, we show that three gene clusters encoding globoside binding have a very similar structure and gene organization, although they were cloned from different E. coli isolates. (asm.org)
The operons encoding for afa afimbrial adhesins were identified in 12% of isolates. (nih.gov)
Fifty seven isolates of thermotolerant E. coli were recovered from 188 drinking water sources, 45 (78.9%) were typable of which 15 (26.3%) were pathogenic serotypes. (springer.com)
Distribution of drb genes coding for Dr binding adhesins among uropathogenic and fecal Escherichia coli isolates and identification of new subtypes. (ebi.ac.uk)

Shear-enhanced binding of intestinal colonization factor antigen I of enterotoxigenic Escherichia coli. (semanticscholar.org)
Enterotoxigenic Escherichia coli TibA glycoprotein adheres to human intestine epithelial cells. (springer.com)

Adherence to epithelial cells by specific adhesins is a characteristic of Shiga toxin-producing Escherichia coli (STEC) strains. (semanticscholar.org)
here we demonstrate that some of these STEC strains are eae-negative and still adhere to epithelial cells at a level 100-fold higher than enterohaemorrhagic E. coli (EHEC) O157 : H7 prototype strain EDL933. (semanticscholar.org)
Adhesins of Escherichia coli associated with extra-intestinal pathogenicity confer binding to colonic epithelial cells. (gu.se)

Transcriptional organization of the F1845 fimbrial adhesin determinant of Escherichia coli. (nih.gov)
The transcriptional organization of the gene cluster encoding the F1845 fimbrial adhesin of a diarrhoea-associated Escherichia coli was investigated. (nih.gov)
The nucleotide sequence of daaA and that of an upstream open reading frame encoded on the opposite strand, designated daaF, were determined to share limited homology with the papB and papI genes of the P fimbrial adhesin, respectively. (nih.gov)

Molecular structure of the Dr adhesin: nucleotide sequence and mapping of receptor-binding domain by use of fusion constructs. (ebi.ac.uk)
Exploring the 3D molecular architecture of Escherichia coli type 1 pili. (nature.com)
In molecular biology, trimeric autotransporter adhesins (TAAs), are proteins found on the outer membrane of Gram-negative bacteria. (wikipedia.org)

Taken together, the results that we present expand our knowledge about a regulatory network for different adhesin gene systems in uropathogenic E. coli and suggest a hierarchy for expression of the fimbrial adhesins. (diva-portal.org)
Factor Afa (encoded by afa gene) is an afimbrial adhesin, and together with P and S fimbriae, have been epidemiologically related to E. coli strains that cause UTI in humans and pets (4). (scielo.br)
Major differences between the adhesin clones were restricted to the central part of the pilin gene (papA) and to one of the two adhesin gene (papG). (asm.org)
the two remaining ones shared identity to a gene encoding a hypothetical protein of uropathogenic E. coli. (semanticscholar.org)
Suitable primers were designed based on specific gene fimA of Salmonella and gene afa of pathogenic E. coli for amplification. (biomedsearch.com)

Although described as an afimbrial adhesin, we show that both AfaE-1 and EPEC Afa possess fine fibrillar structures. (asm.org)

Trimeric autotransporter adhesins have a unique structure. (wikipedia.org)

Escherichia coli is the most common pathogen associated with urinary tract infection (UTI) (5, 10, 30). (scielo.br)
150 women with urinary tract infection caused by Escherichia coli randomly allocated into three groups. (bmj.com)
Women who had a urinary tract infection caused by Escherichia coli (≥10 5 colony forming units/ml in clean voided midstream urine) and were not taking antimicrobial prophylaxis were invited to participate. (bmj.com)
Since common urinary tract-infection bacteria such as E. coli dislike acidic surroundings, physicians concluded that they had discovered a scientific explanation for the traditional uses of cranberry. (epnet.com)

Among them, chaperone-usher (CU) fimbriae adhesins, related to prototypical type 1 fimbriae, interact in highly specific ways with different ligands at different stages of bacterial infection or surface colonisation. (semanticscholar.org)
AIDA is a potent bacterial adhesin associated with some diarrheagenic E. coli strains. (forskningsdatabasen.dk)
Adhesion and bacterial adhesins are also a potential target for prophylaxis or treatment of bacterial infections. (wikipedia.org)
In the crudest sense, bacterial adhesins serve as anchors allowing bacteria to overcome these environmental shear forces, thus remaining in their desired environment. (wikipedia.org)
However, bacterial adhesins do not serve as a sort of universal bacterial Velcro. (wikipedia.org)
Bacterial adhesins provide species and tissue tropism. (wikipedia.org)
Today many different types and subclasses of bacterial adhesins may be observed in the literature. (wikipedia.org)
The bacterial adhesion consists primarily of an intramembranous structural protein which provides a scaffold upon which several extracellular adhesins may be attached. (wikipedia.org)
This basic structure is conserved across type 1 fimbrial adhesins though recent studies have shown that in vitro induced mutations can lead to the addition of C-terminal domain specificity resulting in a bacterial adhesion with dual bending sites and related binding phenotypes. (wikipedia.org)
A large number of bacterial adhesins with individual receptor specificities have been identified. (wikipedia.org)
Many bacterial pathogens are able to express an array of different adhesins. (wikipedia.org)
This has led to the exploration of adhesin activity interruption as a method of bacterial infection treatment. (wikipedia.org)

Fimbrial adhesins are particularly important for the initial establishment of infection in the urinary tract. (diva-portal.org)
Biofilms protect pathogens from attack by the immune system, complicate chronic infections that are difficult to eliminate with antibiotic therapy, and are involved in prostatitis, biliary tract infection, and urinary catheter cystitis caused by Escherichia coli ( 10 , 18 ). (asm.org)
2000). Pathogenic mechanism of mouse brain damage caused by oral infection with shiga toxin producing Escherichia coli 0157: H7. (springer.com)
Additionally, Adhesins are attractive vaccine candidates because they are often essential to infection and are surface-located, making them readily accessible to antibodies. (wikipedia.org)

Localization of EPEC DA adhesin genes. (asm.org)
Although some strains of E. coli synthesize cellulose and the biosynthetic genes are present in E. coli K-12, this strain does not synthesize cellulose under known conditions ( 70 ). (asm.org)

Colanic acid of E. coli affects biofilm architecture but not the adhesion of bacteria to surfaces or to themselves ( 14 , 32 ). (asm.org)
Thus, UpaG is a novel trimeric AT adhesin from E. coli that mediates aggregation, biofilm formation, and adhesion to various ECM proteins. (edu.au)
Adhesins are cell-surface components or appendages of bacteria that facilitate adhesion or adherence to other cells or to surfaces, usually the host they are infecting or living in. (wikipedia.org)
This adhesin is responsible for D-mannose sensitive adhesion. (wikipedia.org)

Here, we show that AIDA possesses self-association characteristics and can mediate autoaggregation of E. coli cells. (forskningsdatabasen.dk)
Binding to HT-29 cells paralleled binding to colonic enterocytes for all adhesin specificities except CFA/I. The results suggest that Dr hemagglutinin, P-, type 1- and S-fimbrial adhesins mediate binding to both colonic and ileal enterocytes. (gu.se)

We investigated the intestinal adherence of E. coli with Dr hemagglutinin, S fimbriae, CFA/I or CFA/II, using freshly isolated ileal or colonic enterocytes and cells from the human colonic cell line HT-29. (gu.se)
To effectively achieve adherence to host surfaces, many bacteria produce multiple adherence factors called adhesins. (wikipedia.org)

Diarrheagenic Escherichia coli . (springer.com)

Crystal structure and mutational analysis of the DaaE adhesin of Escherichia coli. (ebi.ac.uk)

Isolation of E. coli 0157 from raw meat products. (springer.com)

In vivo bioluminescence imaging for the study of intestinal colonization by Escherichia coli in mice. (semanticscholar.org)

Previous studies of the regulation of Escherichia coli biofilm formation suggested the involvement of an unknown adhesin. (asm.org)
The length of the O-antigenic side chain of lipopolysaccharide is inversely correlated with biofilm formation by Salmonella enterica ( 45 ) and E. coli (X. Wang and T. Romeo, unpublished data). (asm.org)
Staphylococcus epidermidis and S. aureus produce β-1,6- N -acetyl- d -glucosamine polymers (β-1,6-GlcNAc) that serve as adhesins and are required for biofilm formation. (asm.org)
Additionally, AIDA expression dramatically enhances biofilm formation by E. coli on abiotic surfaces in How chambers. (forskningsdatabasen.dk)

Binding of Dr adhesins of Escherichia coli to carcinoembryonic antigen triggers receptor dissociation. (ebi.ac.uk)

Duguid JP, Clegg S, Wilson MI (1979) The fimbrial and non-fimbrial hemagglutinins of Escherichia coli . (springer.com)

This is the first characterization and demonstration of an Afa adhesin associated with EPEC. (asm.org)

Prevalence analysis revealed that upaG is strongly associated with E. coli strains from the B2 and D phylogenetic groups, while deletion of upaG had no significant effect on the ability of CFT073 to colonize the mouse urinary tract. (edu.au)

E. coli O55 is one of the most frequently isolated serogroups of EPEC implicated in infantile diarrhea ( 11 ). (asm.org)

In this study the development of a PCR-based technique for the rapid identification of the food-borne pathogens Salmonella and Escherichia coli was undertaken. (biomedsearch.com)

Escherichia coli 0157: H7 and other enterohaemorrhagic E. coli . (springer.com)

Pathogenic strains of Escherichia coli are the most common bacteria associated with urinary tract infections in both humans and companion animals. (scielo.br)
Therefore, we propose that PGA serves as an adhesin that stabilizes biofilms of E. coli and other bacteria. (asm.org)
Adhesins are expressed by both pathogenic bacteria and saprophytic bacteria. (wikipedia.org)
The study of adhesins as a point of exploitation for vaccines comes from early studies which indicated that an important component of protective immunity against certain bacteria came from an ability to prevent adhesin binding. (wikipedia.org)

These specificities may contribute to the establishment of E. coli in the intestinal microflora, which precedes their spread to extra-intestinal sites. (gu.se)

These studies have established that fimA and afa primers were specific for detecting Salmonella and pathogenic E. coli, respectively, in the environmental samples. (biomedsearch.com)

11) reported that pathogenic E. coli strains may pose a risk to either humans or animals. (scielo.br)
Humans and dogs could acquire pathogenic E. coli strains from one another. (scielo.br)

E. coli 0157: H7 associated with human infections in the United Kingdom. (springer.com)

Hybridization with a DNA probe ( daaC probe) developed to detect DA E. coli (DAEC) ( 5 ) indicated that such strains possess a specific DA adhesin. (asm.org)