Escherichia coli
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Adhesins, Escherichia coli
Adhesins, Bacterial
Cell-surface components or appendages of bacteria that facilitate adhesion (BACTERIAL ADHESION) to other cells or to inanimate surfaces. Most fimbriae (FIMBRIAE, BACTERIAL) of gram-negative bacteria function as adhesins, but in many cases it is a minor subunit protein at the tip of the fimbriae that is the actual adhesin. In gram-positive bacteria, a protein or polysaccharide surface layer serves as the specific adhesin. What is sometimes called polymeric adhesin (BIOFILMS) is distinct from protein adhesin.
Escherichia coli O157
A verocytotoxin-producing serogroup belonging to the O subfamily of Escherichia coli which has been shown to cause severe food-borne disease. A strain from this serogroup, serotype H7, which produces SHIGA TOXINS, has been linked to human disease outbreaks resulting from contamination of foods by E. coli O157 from bovine origin.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Base Sequence
Plasmids
Amino Acid Sequence
Escherichia coli K12
Cloning, Molecular
Bacterial Adhesion
Mutation
Gene Expression Regulation, Bacterial
Operon
Fimbriae, Bacterial
Thin, hairlike appendages, 1 to 20 microns in length and often occurring in large numbers, present on the cells of gram-negative bacteria, particularly Enterobacteriaceae and Neisseria. Unlike flagella, they do not possess motility, but being protein (pilin) in nature, they possess antigenic and hemagglutinating properties. They are of medical importance because some fimbriae mediate the attachment of bacteria to cells via adhesins (ADHESINS, BACTERIAL). Bacterial fimbriae refer to common pili, to be distinguished from the preferred use of "pili", which is confined to sex pili (PILI, SEX).
Bacterial Outer Membrane Proteins
RNA, Bacterial
Chromosomes, Bacterial
Genetic Complementation Test
Fimbriae Proteins
Enterotoxigenic Escherichia coli
Strains of ESCHERICHIA COLI that produce or contain at least one member of either heat-labile or heat-stable ENTEROTOXINS. The organisms colonize the mucosal surface of the small intestine and elaborate their enterotoxins causing DIARRHEA. They are mainly associated with tropical and developing countries and affect susceptible travelers to those places.
Sequence Homology, Amino Acid
Enteropathogenic Escherichia coli
Strains of ESCHERICHIA COLI characterized by attaching-and-effacing histopathology. These strains of bacteria intimately adhere to the epithelial cell membrane and show effacement of microvilli. In developed countries they are associated with INFANTILE DIARRHEA and infantile GASTROENTERITIS and, in contrast to ETEC strains, do not produce ENDOTOXINS.
Genes
Restriction Mapping
Shiga-Toxigenic Escherichia coli
Binding Sites
Culture Media
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Bacterial Toxins
Temperature
Conjugation, Genetic
A parasexual process in BACTERIA; ALGAE; FUNGI; and ciliate EUKARYOTA for achieving exchange of chromosome material during fusion of two cells. In bacteria, this is a uni-directional transfer of genetic material; in protozoa it is a bi-directional exchange. In algae and fungi, it is a form of sexual reproduction, with the union of male and female gametes.
Enterohemorrhagic Escherichia coli
Electrophoresis, Polyacrylamide Gel
Substrate Specificity
Uropathogenic Escherichia coli
Virulence
Transcription, Genetic
Virulence Factors
Those components of an organism that determine its capacity to cause disease but are not required for its viability per se. Two classes have been characterized: TOXINS, BIOLOGICAL and surface adhesion molecules that effect the ability of the microorganism to invade and colonize a host. (From Davis et al., Microbiology, 4th ed. p486)
Escherichia
Diarrhea
Protein Binding
Urinary Tract Infections
Recombinant Fusion Proteins
Models, Molecular
Protein Conformation
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
Sequence Analysis, DNA
Feces
Drug Resistance, Microbial
Carrier Proteins
Enterotoxins
Mutagenesis, Site-Directed
beta-Galactosidase
Chromosome Mapping
Escherichia coli Vaccines
Sequence Alignment
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Membrane Transport Proteins
Bacteriophage lambda
Chloramphenicol
An antibiotic first isolated from cultures of Streptomyces venequelae in 1947 but now produced synthetically. It has a relatively simple structure and was the first broad-spectrum antibiotic to be discovered. It acts by interfering with bacterial protein synthesis and is mainly bacteriostatic. (From Martindale, The Extra Pharmacopoeia, 29th ed, p106)
Membrane Proteins
Hemagglutination
Species Specificity
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
DNA-Directed RNA Polymerases
Enzymes that catalyze DNA template-directed extension of the 3'-end of an RNA strand one nucleotide at a time. They can initiate a chain de novo. In eukaryotes, three forms of the enzyme have been distinguished on the basis of sensitivity to alpha-amanitin, and the type of RNA synthesized. (From Enzyme Nomenclature, 1992).
Transduction, Genetic
Hydrogen-Ion Concentration
DNA Transposable Elements
Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.
Polymerase Chain Reaction
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Mutagenesis
Colicins
DNA Restriction Enzymes
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
Genetics, Microbial
Cell Membrane
Shiga Toxin 1
Galactosidases
DNA Primers
Lac Operon
Adenosine Triphosphatases
Hemagglutinins
Protein Structure, Tertiary
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
SOS Response (Genetics)
An error-prone mechanism or set of functions for repairing damaged microbial DNA. SOS functions (a concept reputedly derived from the SOS of the international distress signal) are involved in DNA repair and mutagenesis, in cell division inhibition, in recovery of normal physiological conditions after DNA repair, and possibly in cell death when DNA damage is extensive.
Promoter Regions, Genetic
Transformation, Bacterial
Recombination, Genetic
Pyelonephritis
Nucleic Acid Conformation
Salmonella typhimurium
Genes, Regulator
Ribosomes
beta-Lactamases
Sequence Homology, Nucleic Acid
Mutagenesis, Insertional
Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene or extragenic sequence. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA insertions into or adjacent to a cellular proto-oncogene can interrupt GENETIC TRANSLATION of the coding sequences or interfere with recognition of regulatory elements and cause unregulated expression of the proto-oncogene resulting in tumor formation.
Lysogeny
The phenomenon by which a temperate phage incorporates itself into the DNA of a bacterial host, establishing a kind of symbiotic relation between PROPHAGE and bacterium which results in the perpetuation of the prophage in all the descendants of the bacterium. Upon induction (VIRUS ACTIVATION) by various agents, such as ultraviolet radiation, the phage is released, which then becomes virulent and lyses the bacterium.
Periplasmic Binding Proteins
Rec A Recombinases
A family of recombinases initially identified in BACTERIA. They catalyze the ATP-driven exchange of DNA strands in GENETIC RECOMBINATION. The product of the reaction consists of a duplex and a displaced single-stranded loop, which has the shape of the letter D and is therefore called a D-loop structure.
Anaerobiosis
Crystallography, X-Ray
Suppression, Genetic
Mutation process that restores the wild-type PHENOTYPE in an organism possessing a mutationally altered GENOTYPE. The second "suppressor" mutation may be on a different gene, on the same gene but located at a distance from the site of the primary mutation, or in extrachromosomal genes (EXTRACHROMOSOMAL INHERITANCE).
Structure-Activity Relationship
Shiga Toxin 2
Phenotype
Gene Deletion
Maltose-Binding Proteins
Enterobacteriaceae
A family of gram-negative, facultatively anaerobic, rod-shaped bacteria that do not form endospores. Its organisms are distributed worldwide with some being saprophytes and others being plant and animal parasites. Many species are of considerable economic importance due to their pathogenic effects on agriculture and livestock.
DNA, Recombinant
Meningitis, Escherichia coli
A form of gram-negative meningitis that tends to occur in neonates, in association with anatomical abnormalities (which feature communication between the meninges and cutaneous structures) or as OPPORTUNISTIC INFECTIONS in association with IMMUNOLOGIC DEFICIENCY SYNDROMES. In premature neonates the clinical presentation may be limited to ANOREXIA; VOMITING; lethargy; or respiratory distress. Full-term infants may have as additional features FEVER; SEIZURES; and bulging of the anterior fontanelle. (From Menkes, Textbook of Child Neurology, 5th ed, pp398-400)
Gene Expression
RNA, Transfer
The small RNA molecules, 73-80 nucleotides long, that function during translation (TRANSLATION, GENETIC) to align AMINO ACIDS at the RIBOSOMES in a sequence determined by the mRNA (RNA, MESSENGER). There are about 30 different transfer RNAs. Each recognizes a specific CODON set on the mRNA through its own ANTICODON and as aminoacyl tRNAs (RNA, TRANSFER, AMINO ACYL), each carries a specific amino acid to the ribosome to add to the elongating peptide chains.
Serotyping
Lactose
Sigma Factor
Cattle
Amino Acids
Ribosomal Proteins
Microbial Sensitivity Tests
Shiga Toxins
A class of toxins that inhibit protein synthesis by blocking the interaction of ribosomal RNA; (RNA, RIBOSOMAL) with PEPTIDE ELONGATION FACTORS. They include SHIGA TOXIN which is produced by SHIGELLA DYSENTERIAE and a variety of shiga-like toxins that are produced by pathologic strains of ESCHERICHIA COLI such as ESCHERICHIA COLI O157.
Oxidoreductases
The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)
Open Reading Frames
Protein Biosynthesis
Hemolysin Proteins
Biofilms
Encrustations, formed from microbes (bacteria, algae, fungi, plankton, or protozoa) embedding in extracellular polymers, that adhere to surfaces such as teeth (DENTAL DEPOSITS); PROSTHESES AND IMPLANTS; and catheters. Biofilms are prevented from forming by treating surfaces with DENTIFRICES; DISINFECTANTS; ANTI-INFECTIVE AGENTS; and antifouling agents.
Drug Resistance, Bacterial
F Factor
Phosphotransferases
Colony Count, Microbial
Enumeration by direct count of viable, isolated bacterial, archaeal, or fungal CELLS or SPORES capable of growth on solid CULTURE MEDIA. The method is used routinely by environmental microbiologists for quantifying organisms in AIR; FOOD; and WATER; by clinicians for measuring patients' microbial load; and in antimicrobial drug testing.
Bacteria
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
DNA-Binding Proteins
Macromolecular Substances
Protein Structure, Secondary
Adenosine Triphosphate
Ultraviolet Rays
That portion of the electromagnetic spectrum immediately below the visible range and extending into the x-ray frequencies. The longer wavelengths (near-UV or biotic or vital rays) are necessary for the endogenous synthesis of vitamin D and are also called antirachitic rays; the shorter, ionizing wavelengths (far-UV or abiotic or extravital rays) are viricidal, bactericidal, mutagenic, and carcinogenic and are used as disinfectants.
R Factors
Isopropyl Thiogalactoside
Periplasm
Nalidixic Acid
Streptomycin
Codon
A set of three nucleotides in a protein coding sequence that specifies individual amino acids or a termination signal (CODON, TERMINATOR). Most codons are universal, but some organisms do not produce the transfer RNAs (RNA, TRANSFER) complementary to all codons. These codons are referred to as unassigned codons (CODONS, NONSENSE).
Biological Transport
O Antigens
The lipopolysaccharide-protein somatic antigens, usually from gram-negative bacteria, important in the serological classification of enteric bacilli. The O-specific chains determine the specificity of the O antigens of a given serotype. O antigens are the immunodominant part of the lipopolysaccharide molecule in the intact bacterial cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Enzyme Stability
Catalysis
Nucleic Acid Hybridization
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
Food Microbiology
Microscopy, Electron
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
DNA Repair
The reconstruction of a continuous two-stranded DNA molecule without mismatch from a molecule which contained damaged regions. The major repair mechanisms are excision repair, in which defective regions in one strand are excised and resynthesized using the complementary base pairing information in the intact strand; photoreactivation repair, in which the lethal and mutagenic effects of ultraviolet light are eliminated; and post-replication repair, in which the primary lesions are not repaired, but the gaps in one daughter duplex are filled in by incorporation of portions of the other (undamaged) daughter duplex. Excision repair and post-replication repair are sometimes referred to as "dark repair" because they do not require light.
Carbon Isotopes
Spheroplasts
Klebsiella pneumoniae
Repressor Proteins
Porins
Porins are protein molecules that were originally found in the outer membrane of GRAM-NEGATIVE BACTERIA and that form multi-meric channels for the passive DIFFUSION of WATER; IONS; or other small molecules. Porins are present in bacterial CELL WALLS, as well as in plant, fungal, mammalian and other vertebrate CELL MEMBRANES and MITOCHONDRIAL MEMBRANES.
Genotype
Shigella
Cell-Free System
A fractionated cell extract that maintains a biological function. A subcellular fraction isolated by ultracentrifugation or other separation techniques must first be isolated so that a process can be studied free from all of the complex side reactions that occur in a cell. The cell-free system is therefore widely used in cell biology. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p166)
Hemolytic-Uremic Syndrome
RNA, Ribosomal
The most abundant form of RNA. Together with proteins, it forms the ribosomes, playing a structural role and also a role in ribosomal binding of mRNA and tRNAs. Individual chains are conventionally designated by their sedimentation coefficients. In eukaryotes, four large chains exist, synthesized in the nucleolus and constituting about 50% of the ribosome. (Dorland, 28th ed)
DNA
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Heat-Shock Proteins
Genetic Engineering
Multienzyme Complexes
Serine Endopeptidases
Swine
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
Maltose
Shiga Toxin
Lipopolysaccharides
Lipid-containing polysaccharides which are endotoxins and important group-specific antigens. They are often derived from the cell wall of gram-negative bacteria and induce immunoglobulin secretion. The lipopolysaccharide molecule consists of three parts: LIPID A, core polysaccharide, and O-specific chains (O ANTIGENS). When derived from Escherichia coli, lipopolysaccharides serve as polyclonal B-cell mitogens commonly used in laboratory immunology. (From Dorland, 28th ed)
Pseudomonas aeruginosa
Hydro-Lyases
Lyases
Tryptophan
An essential amino acid that is necessary for normal growth in infants and for NITROGEN balance in adults. It is a precursor of INDOLE ALKALOIDS in plants. It is a precursor of SEROTONIN (hence its use as an antidepressant and sleep aid). It can be a precursor to NIACIN, albeit inefficiently, in mammals.
Mannose
Crystallization
Amino Acyl-tRNA Synthetases
Genetic Vectors
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
Oxidation-Reduction
A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).
Hemagglutination Tests
Molecular basis for the enterocyte tropism exhibited by Salmonella typhimurium type 1 fimbriae. (1/525)
Salmonella typhimurium exhibits a distinct tropism for mouse enterocytes that is linked to their expression of type 1 fimbriae. The distinct binding traits of Salmonella type 1 fimbriae is also reflected in their binding to selected mannosylated proteins and in their ability to promote secondary bacterial aggregation on enterocyte surfaces. The determinant of binding in Salmonella type 1 fimbriae is a 35-kDa structurally distinct fimbrial subunit, FimHS, because inactivation of fimHS abolished binding activity in the resulting mutant without any apparent effect on fimbrial expression. Surprisingly, when expressed in the absence of other fimbrial components and as a translational fusion protein with MalE, FimHS failed to demonstrate any specific binding tropism and bound equally to all cells and mannosylated proteins tested. To determine if the binding specificity of Salmonella type 1 fimbriae was determined by the fimbrial shaft that is intimately associated with FimHS, we replaced the amino-terminal half of FimHS with the corresponding sequence from Escherichia coli FimH (FimHE) that contains the receptor binding domain of FimHE. The resulting hybrid fimbriae bearing FimHES on a Salmonella fimbrial shaft exhibited binding traits that resembled that of Salmonella rather than E. coli fimbriae. Apparently, the quaternary constraints imposed by the fimbrial shaft on the adhesin determine the distinct binding traits of S. typhimurium type 1 fimbriae. (+info)P fimbriae and other adhesins enhance intestinal persistence of Escherichia coli in early infancy. (2/525)
Resident and transient Escherichia coli strains were identified in the rectal flora of 22 Pakistani infants followed from birth to 6 months of age. All strains were tested for O-antigen expression, adhesin specificity (P fimbriae, other mannose-resistant adhesins or type 1 fimbriae) and adherence to the colonic cell line HT-29. Resident strains displayed higher mannose-resistant adherence to HT-29 cells, and expressed P fimbriae (P = 0.0036) as well as other mannose-resistant adhesins (P = 0.012) more often than transient strains. In strains acquired during the first month of life, P fimbriae were 12 times more frequent in resident than in transient strains (P = 0.0006). The O-antigen distribution did not differ between resident and transient strains, and none of the resident P-fimbriated strains belonged to previously recognized uropathogenic clones. The results suggest that adhesins mediating adherence to intestinal epithelial cells, especially P fimbriae, enhance the persistence of E. coli in the large intestine of infants. (+info)Organization of biogenesis genes for aggregative adherence fimbria II defines a virulence gene cluster in enteroaggregative Escherichia coli. (3/525)
Several virulence-related genes have been described for prototype enteroaggregative Escherichia coli (EAEC) strain 042, which has been shown to cause diarrhea in human volunteers. Among these factors are the enterotoxins Pet and EAST and the fimbrial antigen aggregative adherence fimbria II (AAF/II), all of which are encoded on the 65-MDa virulence plasmid pAA2. Using nucleotide sequence analysis and insertional mutagenesis, we have found that the genes required for the expression of each of these factors, as well as the transcriptional activator of fimbrial expression AggR, map to a distinct cluster on the pAA2 plasmid map. The cluster is 23 kb in length and includes two regions required for expression of the AAF/II fimbria. These fimbrial biogenesis genes feature a unique organization in which the chaperone, subunit, and transcriptional activator lie in one cluster, whereas the second, unlinked cluster comprises a silent chaperone gene, usher, and invasin reminiscent of Dr family fimbrial clusters. This plasmid-borne virulence locus may represent an important set of virulence determinants in EAEC strains. (+info)Adhesins as targets for vaccine development. (4/525)
Blocking the primary stages of infection, namely bacterial attachment to host cell receptors and colonization of the mucosal surface, may be the most effective strategy to prevent bacterial infections. Bacterial attachment usually involves an interaction between a bacterial surface protein called an adhesin and the host cell receptor. Recent preclinical vaccine studies with the FimH adhesin (derived from uropathogenic Escherichia coli) have confirmed that antibodies elicited against an adhesin can impede colonization, block infection, and prevent disease. The studies indicate that prophylactic vaccination with adhesins can block bacterial infections. With recent advances in the identification, characterization, and isolation of other adhesins, similar approaches are being explored to prevent infections, from otitis media and dental caries to pneumonia and sepsis. (+info)The mast cell tumor necrosis factor alpha response to FimH-expressing Escherichia coli is mediated by the glycosylphosphatidylinositol-anchored molecule CD48. (5/525)
Mast cells are well known for their harmful role in IgE-mediated hypersensitivity reactions, but their physiological role remains a mystery. Several recent studies have reported that mast cells play a critical role in innate immunity in mice by releasing tumor necrosis factor alpha (TNF-alpha) to recruit neutrophils to sites of enterobacterial infection. In some cases, the mast cell TNF-alpha response was triggered when these cells directly bound FimH on the surface of Escherichia coli. We have identified CD48, a glycosylphosphatidylinositol-anchored molecule, to be the complementary FimH-binding moiety in rodent mast cell membrane fractions. We showed that (i) pretreatment of mast cell membranes with antibodies to CD48 or phospholipase C inhibited binding of FimH+ E. coli, (ii) FimH+ E. coli but not a FimH- derivative bound isolated CD48 in a mannose-inhibitable manner, (iii) binding of FimH+ bacteria to Chinese hamster ovary (CHO) cells was markedly increased when these cells were transfected with CD48 cDNA, and (iv) antibodies to CD48 specifically blocked the mast cell TNF-alpha response to FimH+ E. coli. Thus, CD48 is a functionally relevant microbial receptor on mast cells that plays a role in triggering inflammation. (+info)Decay-accelerating factor and cytoskeleton redistribution pattern in HeLa cells infected with recombinant Escherichia coli strains expressing Dr family of adhesins. (6/525)
Escherichia coli strains expressing Dr fimbriae are able to enter epithelial cells by interacting with a complement-regulatory protein, decay-accelerating factor. This model of bacterial internalization, with a well-characterized bacterial ligand and host receptor, provides a unique opportunity to investigate the early stages of invasion. We used immunofluorescence staining techniques to examine the distribution of receptor and cytoskeletal proteins in HeLa cells infected with E. coli recombinant strains that expressed Dr family of adhesins: Dr, Dr-II, F1845, AFA-I, and AFA-III. A major rearrangement of decay-accelerating factor was found at the adherence sites of recombinant strains expressing Dr, Dr-II, and F1845 adhesins. The changes in the distribution of receptor were significantly smaller on HeLa cells infected with E. coli bearing AFA-I or AFA-III afimbrial adhesins. Receptor aggregation was associated with the redistribution of cytoskeleton-associated proteins such as actin, alpha-actinin, ezrin, and occasionally tropomyosin. Purified Dr fimbriae coated on polystyrene beads were capable of triggering clustering of receptor and accumulating actin at the adhesion sites of beads to HeLa cells. Using scanning and transmission electron microscopic techniques, we have shown that beads coated with Dr fimbriae, as opposed to beads coated with bovine serum albumin, were enwrapped by cellular microvilli and ultimately internalized into HeLa cells. This indicates that interaction of Dr fimbriae with decay-accelerating factor is associated with redistribution of receptor and is sufficient to promote bacterial internalization. (+info)X-ray structure of the FimC-FimH chaperone-adhesin complex from uropathogenic Escherichia coli. (7/525)
Type 1 pili-adhesive fibers expressed in most members of the Enterobacteriaceae family-mediate binding to mannose receptors on host cells through the FimH adhesin. Pilus biogenesis proceeds by way of the chaperone/usher pathway. The x-ray structure of the FimC-FimH chaperone-adhesin complex from uropathogenic Escherichia coli at 2.5 angstrom resolution reveals the basis for carbohydrate recognition and for pilus assembly. The carboxyl-terminal pilin domain of FimH has an immunoglobulin-like fold, except that the seventh strand is missing, leaving part of the hydrophobic core exposed. A donor strand complementation mechanism in which the chaperone donates a strand to complete the pilin domain explains the basis for both chaperone function and pilus biogenesis. (+info)Virulence characteristics of Escherichia coli in acute bacterial prostatitis. (8/525)
To assess the urovirulence characteristics of Escherichia coli strains causing acute prostatitis, urinary isolates from men with acute prostatitis (n=107) and from women with acute uncomplicated pyelonephritis (n=76) were examined for the prevalence of sfa, foc, and 3 papG allele genotypes and phenotypes and for the production of alpha-hemolysin and cytotoxic necrotizing factor 1. The papG allele III and foc gene were found more frequently and the papG allele II less frequently among prostatitis than from pyelonephritis isolates. A higher proportion of hly+ cnf1+ genotype in prostatitis strains (64% vs. 36%) was particularly striking. Both prostatitis and pyelonephritis strains expressed virulence factors similarly except for a higher proportion of nonhemolytic prostatitis isolates. Although the pathogenetic mechanisms of urinary tract infections in men and women may differ, virulence factors such as adhesins and cytotoxins may have important roles in the pathogenesis of acute prostatitis. (+info)Identification and characterization of the locus for diffuse adherence, which encodes a novel afimbrial adhesin found in...
Adhesive factor/rabbit 2, a new fimbrial adhesin and a virulence factor from Escherichia coli O103, a serogroup...
Improved detection and performance of surface expression from the AIDA-I autotransporter
Autodisplay Biotech GmbH - Screening Systems
Use of a wild-type gene fusion to determine the influence of environmental conditions on expression of the S fimbrial adhesin...
Autodisplay Biotech GmbH - Technology
Papež na sklonku dne by Ralf Dahrendorf - Project Syndicate
Exercise and Electrolytes | Dr Family Fitness
phoA protein, E coli | Semantic Scholar
1 - Diagram fasa binary options
Set Percubaan Fasa 1/2 untuk Menguji ASC618, Terapi Gen untuk Hemofilia A
Publications | SBRC
Maumivu ya nyonga | Chanzo, Vipimo, Ushauri na TIba
Items where Author is Seifert, R. - ePIC
ENERGY-TRANS - Forschung - Publikationen
Aggregative adherence fimbriae II of enteroaggregative Escherichia coli are required for adherence and barrier disruption...
FimH family of type 1 fimbrial adhesins: functional heterogeneity due to minor sequence variations among fimH genes. | Journal...
Adhesion of Enteroaggregative E. coli Strains to HEK293 ...
Misfolding of a bacterial autotransporter - Forschungsinformationssystem | Universität Kassel - CONVERIS...
Process optimization for increased yield of surface-expressed protein in Escherichia coli
The Level of Satisfaction of Family Physician Program in Urban Population of Fasa from 2014 to 2015
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Omptin
Found in the outer membrane of gram-negative enterobacteria such as Shigella flexneri, Yersinia pestis, Escherichia coli, and ... Kukkonen M, Korhonen TK (July 2004). "The omptin family of enterobacterial surface proteases/adhesins: from housekeeping in ... Escherichia coli to systemic spread of Yersinia pestis". Int. J. Med. Microbiol. 294 (1): 7-14. doi:10.1016/j.ijmm.2004.01.003 ...
Lipopolysaccharide
Other studies have shown that purified endotoxin from Escherichia coli can induce obesity and insulin-resistance when injected ... Kukkonen, Maini; Korhonen, Timo K. (July 2004). "The omptin family of enterobacterial surface proteases/adhesins: from ... housekeeping in Escherichia coli to systemic spread of Yersinia pestis". International Journal of Medical Microbiology. 294 (1 ... Bacterial genera associated with endotoxin-related obesity effects include Escherichia and Enterobacter. There is experimental ...
N-glycosyltransferase
Escherichia coli and Burkholderia sp.) is linked to trimeric autotransporter adhesins and the second has enzymes genomically ... and later in other bacterial species such as Escherichia coli. N-glycosyltransferases usually target adhesin proteins, which ... Enterotoxigenic Escherichia coli uses a N-glycosyltransferase called EtpC to modify the EtpA protein, which is orthologous to ... Other homologues have been found in Burkholderia species, Escherichia coli, Haemophilus ducreyi, Mannheimia species, ...
Jan Maree Tennent
"The PapG adhesin of uropathogenic Escherichia coli contains separate regions for receptor binding and for the incorporation ... She researched the genetic mechanism and control of host attachment by uropathogenic Escherichia coli as part of the Normark ... Tennent, J. M.; Lindberg, F.; Normark, S. (May 1990). "Integrity of Escherichia coli P pili during biogenesis: properties and ... "Horizontal gene transfer of the Escherichia coli pap and prs pili operons as a mechanism for the development of tissue-specific ...
Staphylococcus saprophyticus
September 2012). "Effects of cranberry extracts on growth and biofilm production of Escherichia coli and Staphylococcus species ... The adhesin for S. saprophyticus is a lactosamine structure. S. saprophyticus produces no exotoxins. Patients with urinary ... after Escherichia coli. Sexual activity increases the risk of S. saprophyticus UTIs because bacteria are displaced from the ...
List of MeSH codes (D12.776.097)
MeSH D12.776.097.120.050.040 - adhesins, escherichia coli MeSH D12.776.097.120.300.500 - transferrin-binding protein a MeSH ...
List of MeSH codes (D23)
MeSH D23.050.161.050 - adhesins, bacterial MeSH D23.050.161.050.040 - adhesins, escherichia coli MeSH D23.050.161.386 - ...
List of MeSH codes (D12.776.543)
MeSH D12.776.543.100.050.040 - adhesins, escherichia coli MeSH D12.776.543.325.100.100 - gtp-binding protein alpha subunits, ...
Virulence factor
Bacteria like Escherichia coli O157:H7 gain the majority of their virulence from mobile genetic elements. Gram-negative ... Bacteria produce various adhesins including lipoteichoic acid, trimeric autotransporter adhesins and a wide variety of other ... Exotoxins are also produced by a range of other bacteria including Escherichia coli; Vibrio cholerae (causative agent of ... These factors include adhesins, invasins, and antiphagocytic factors. The factors, including toxins, hemolysins and proteases, ...
Fim switch
The fim switch in Escherichia coli is the mechanism by which the fim gene cluster, encoding Type I Pili, is transcriptionally ... coding for an adhesin at the tip, to name just a few important elements. The fim S region is flanked by 9bp repeats that are ... Klemm, P (1986). "Two regulatory fim genes, fimB and fimE, control the phase variation of type 1 fimbriae in Escherichia coli ... These pili are virulence factors involved in adhesion, especially important in uropathogenic Escherichia coli. The gene ...
Pathogenic Escherichia coli
The Dr adhesins bind Dr blood group antigen (Dra) which is present on decay accelerating factor (DAF) on erythrocytes and other ... coli. List of strains of Escherichia coli "Escherichia coli O157:H7". CDC Division of Bacterial and Mycotic Diseases. Retrieved ... "Escherichia coli in chronic inflammatory bowel diseases: An update on adherent invasive Escherichia coli pathogenicity". World ... Escherichia coli (Latin pronunciation: [eskeˈrikja ˈkoli] Anglicized to /ˌɛʃəˈrɪkiə ˈkoʊlaɪ/; commonly abbreviated E. coli) is ...
Enteroaggregative Escherichia coli
... typically associated with Shiga toxin-producing Escherichia coli, which often encode the adhesin intimin). The putative cause ... coli (EPEC), enterotoxigenic E.coli (ETEC), Shiga toxin-producing E. coli (STEC) and Enteroaggregative E. coli (EAEC). E. coli ... Enteroaggregative Escherichia coli (EAEC or EAggEC) are a pathotype of Escherichia coli which cause acute and chronic diarrhea ... Nataro, James P.; Steiner, Theodore (2002), "Enteroaggregative and Diffusely Adherent Escherichia Coli", Escherichia Coli, ...
Enteroinvasive Escherichia coli
EIEC are highly invasive, and they use adhesin proteins to bind to and enter intestinal cells. They produce no toxins, but ... "Shigella and enteroinvasive Escherichia coli strains are derived from distinct ancestral strains of E. coli". Microbiology. 144 ... Enteroinvasive Escherichia coli (EIEC) is a type of pathogenic bacteria whose infection causes a syndrome that is identical to ... Escherichia coli, enteroinvasive Material Data Safety Sheets (Articles with short description, Short description is different ...
Alpha-D-phosphohexomutase superfamily
"Characterization of the essential gene glmM encoding phosphoglucosamine mutase in Escherichia coli". The Journal of Biological ... "The Haemophilus influenzae HMW1 adhesin is glycosylated in a process that requires HMW1C and phosphoglucomutase, an enzyme ... "Molecular cloning and characterization of the pgm gene encoding phosphoglucomutase of Escherichia coli". Journal of ... Enzymes from this superfamily are ubiquitous in organisms from E. Coli to humans, and catalyze a phosphoryl transfer reaction ...
Bacterial adhesin
The effectiveness of anti-adhesin antibodies is illustrated by studies with FimH, the adhesin of uropathogenic Escherichia coli ... Schembri MA, Klemm P (May 1998). "Heterobinary adhesins based on the Escherichia coli FimH fimbrial protein". Appl. Environ. ... August 1999). "X-ray structure of the FimC-FimH chaperone-adhesin complex from uropathogenic Escherichia coli". Science. 285 ( ... October 2011). "Type 1 fimbrial adhesin FimH elicits an immune response that enhances cell adhesion of Escherichia coli". ...
Trimeric autotransporter adhesin
2008). "UpaG, a new member of the trimeric autotransporter family of adhesins in uropathogenic Escherichia coli". J Bacteriol. ... YadA stands for Yersinia adhesin protein A. This protein domain is an example of Trimeric Autotransporter Adhesins, and it was ... Trimeric autotransporter adhesins have a unique structure. The structure they hold is crucial to their function. They all ... All Trimeric Autotransporter Adhesins are crucial virulence factors that cause serious disease in humans. The most-studied and ...
OmpA domain
OmpA from Escherichia coli is required for pathogenesis, and can interact with host receptor molecules. MotB (and MotA) serve ... De Mot R, Proost P, Van Damme J, Vanderleyden J (February 1992). "Homology of the root adhesin of Pseudomonas fluorescens OE ... Freudl R, Klose M, Henning U (June 1990). "Export and sorting of the Escherichia coli outer membrane protein OmpA". J. Bioenerg ... Hosking ER, Vogt C, Bakker EP, Manson MD (December 2006). "The Escherichia coli MotAB proton channel unplugged". J. Mol. Biol. ...
YadA bacterial adhesin protein domain
The Eib immunoglobulin-binding proteins from Escherichia coli were third, followed by the DsrA proteins of Haemophilus ducreyi ... and IgG Fc by distinct sequence segments of the EibF cell surface protein of Escherichia coli". Infect. Immun. 69 (12): 7293- ... YadA, an adhesin from Yersinia, was the first member of this family to be characterised. UspA2 from Moraxella was second. ... The importance of adhesins to YadA function and Yersinia survival is huge. Attachment further allows more interactions and ...
Edward McSweegan
"Identification and characterization of mouse small intestine mucosal receptors for Escherichia coli K-12(K88ab)". Infection and ... McSweegan, E; Walker, R I (1986). "Identification and characterization of two Campylobacter jejuni adhesins for cellular and ... published research on the disease-causing mechanisms of the bacteria Campylobacter jejuni and Enterotoxigenic Escherichia coli ...
Antibiotic
July 2016). "Escherichia coli Harboring mcr-1 and blaCTX-M on a Novel IncF Plasmid: First Report of mcr-1 in the United States ... adhesins, internalins), coordinate the activation of virulence genes (e.g. quorum sensing), and cause disease (e.g. exotoxins ... For example, glucose, mannitol, and fructose reduce antibiotic tolerance in Escherichia coli and Staphylococcus aureus, ... "Antimicrobial Activity of Selected Phytochemicals against Escherichia coli and Staphylococcus aureus and Their Biofilms". ...
Bacterial display
Freudl R, MacIntyre S, Degen M, Henning U (1986). "Cell Surface Exposure of the Outer Membrane Protein OmpA of Escherichia coli ... Klemm P, Schembri MA (2000). "Bacterial Adhesins:Function and Structure". Int J Med Microbiol. 290 (1): 27-35. doi:10.1016/ ... Wang Y (2002). "The Function of OmpA in Escherichia coli". Biochem Biophys Res Commun. 292 (2): 396-401. doi:10.1006/bbrc. ... Many types of bacteria have cell surface proteins such as the enteropathogenic E. coli intimin protein which is involved in ...
Synthetic biology
Gardner TS, Cantor CR, Collins JJ (January 2000). "Construction of a genetic toggle switch in Escherichia coli". Nature. 403 ( ... coli to target surfaces, cells, and tumors with synthetic adhesins". ACS Synthetic Biology. 4 (4): 463-73. doi:10.1021/ ... 15 May 2019). "Total synthesis of Escherichia coli with a recoded genome". Nature. 569 (7757): 514-518. Bibcode:2019Natur.569.. ... 2005). ""Synthetic biology " engineering Escherichia coli to see light". Nature. 438 (7067): 441-442. Bibcode:2005Natur.438.. ...
Haemolysin expression modulating protein family
This family consists of haemolysin expression modulating protein (Hha) from Escherichia coli and its enterobacterial homologues ... YmoA modulates the expression of various virulence factors, such as Yop proteins and YadA adhesin, in response to temperature. ...
Dispersin B
... in Staphylococcus species and PGA in Escherichia coli. By degrading the biofilm matrix, Dispersin B allows for the release of ... Mack D, Fischer W, Krokotsch A, Leopold K, Hartmann R, Egge H, Laufs R (1996). "The intercellular adhesin involved in biofilm ... The three-dimensional structure of dispersin B was determined by expressing the protein in E. coli, purifying it from cultures ... and proteinaceous adhesins. It allows bacteria to adhere to host surfaces, protects the bacterial cells from host defenses, ...
Sepsis
Other commonly implicated bacteria include Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa, and Klebsiella ... Bacterial virulence factors, such as glycocalyx and various adhesins, allow colonization, immune evasion, and establishment of ... The bacteria Streptococcus pneumoniae, Escherichia coli, Proteus spp., Pseudomonas aeruginosa, Staphylococcus aureus, ...
P fimbriae
"Escherichia coli mediated urinary tract infections: are there distinct uropathogenic E. coli (UPEC) pathotypes?" (PDF). FEMS ... PapGI adhesins bind preferentially to globotriaosylceramide (GbO3), while the isoreceptors of PapGIV are unknown. E. coli ... Johanson IM, Plos K, Marklund BI, Svanborg C (August 1993). "Pap, papG and prsG DNA sequences in Escherichia coli from the ... Wullt B, Bergsten G, Samuelsson M, Svanborg C (June 2002). "The role of P fimbriae for Escherichia coli establishment and ...
Pilus
Cookson, AL; Cooley, WA; Woodward, MJ (2002), "The role of type 1 and curli fimbriae of Shiga toxin-producing Escherichia coli ... They contain FimH adhesins at the "tips". The chaperone-usher pathway is responsible for moving many types of fimbriae out of ... Rice JC, Peng T, Spence JS, Wang HQ, Goldblum RM, Corthésy B, Nowicki BJ (December 2005). "Pyelonephritic Escherichia coli ... Perhaps the most well-studied is the F-pilus of Escherichia coli, encoded by the F sex factor. A sex pilus is typically 6 to 7 ...
Antivirulence
Uropathogenic Escherichia coli (UPEC) is the major aetiological agent of Urinary Tract Infections (UTIs) and is often studied ... are non proteinaceous adhesins like Wall Teichoic acids (WTAs) and lipoteichoic acids. Since WTAs are required for host ... Pilicides inhibit the FGL chaperone/usher assisted biogenesis of thefimbrial polyadhesin from uropathogenic Escherichia coli. ...
Bacterial secretion system
The secreted molecules vary in size from the small Escherichia coli peptide colicin V, which is 10 kDa, to the Pseudomonas ... An example of autotransporter is the Trimeric Autotransporter Adhesins. Type VI secretion systems (T6SS) were discovered by the ... Crane JM, Randall LL (November 2017). "The Sec System: Protein Export in Escherichia coli". EcoSal Plus. 7 (2): ESP-0002-2017. ... "The twin arginine consensus motif of Tat signal peptides is involved in Sec-independent protein targeting in Escherichia coli ...
Mycoplasma pneumoniae
This is in contrast to another model organism, Escherichia coli, in which only 15% of its metabolic enzymes are essential. In ... The P1 adhesin (trypsin-sensitive protein) is a 120 kDa protein highly clustered on the surface of the attachment organelle tip ... This may be due to the fact that Mycoplasma pneumoniae's metabolome is less efficient than that of Escherichia coli. The ... Chowdhury S, Hepper S, Lodi MK, Saier MH, Uetz P (April 2021). "The Protein Interactome of Glycolysis in Escherichia coli". ...
Protein tag
Bedouelle, Hugues; Duplay, Pascale (Feb 1988). "Production in Escherichia coli and one-step purification of bifunctional hybrid ... through engineering a bacterial adhesin". Proceedings of the National Academy of Sciences. 109 (12): E690-7. Bibcode:2012PNAS.. ... The iCapTag™-target protein complex can be expressed in a wide range of expression hosts (e.g. CHO and E.coli cells). It is not ... Solubilization tags are used, especially for recombinant proteins expressed in species such as E. coli, to assist in the proper ...
Gabriel Waksman
CU) pili have clear relevance in the pathogenicity of uropathogenic Escherichia coli, where CU pili mediate bacterial tropism ... coli adhesin to its human kidney receptor". Cell. 105 (6): 733-43. doi:10.1016/S0092-8674(01)00388-9. PMID 11440716. S2CID ...
Salmonella
Winfield MD, Groisman EA (July 2003). "Role of nonhost environments in the lifestyles of Salmonella and Escherichia coli". ... In the study by Kisela et al., more pathogenic serovars of S. enterica were found to have certain adhesins in common that have ... in which they adhere to a host cell using bacterial adhesins and a type three-secretion system, Invasion, in which Salmonella ... "Evolution of Salmonella enterica virulence via point mutations in the fimbrial adhesin". PLOS Pathogens. 8 (6): e1002733. doi: ...
Intimin
... is a virulence factor (adhesin) of EPEC (e.g. E. coli O127:H6) and EHEC (e.g. E. coli O157:H7) E. coli strains. It is ... "A cloned pathogenicity island from enteropathogenic Escherichia coli confers the attaching and effacing phenotype on E. coli K- ... Jerse AE, Yu J, Tall BD, Kaper JB (October 1990). "A genetic locus of enteropathogenic Escherichia coli necessary for the ... by intimin from enteropathogenic Escherichia coli". The EMBO Journal. 19 (11): 2452-64. doi:10.1093/emboj/19.11.2452. PMC ...
Type three secretion system
... coli proteins Esc: Escherichia secretion (component) Esp: Escherichia secretion protein Ces: Chaperone of E. coli secretion ... It needs to have a minimal length so that other extracellular bacterial structures (adhesins and the lipopolysaccharide layer, ... Escherichia coli (Gut flora, some strains cause food poisoning), Vibrio (gastroenteritis and diarrhea), Burkholderia (glanders ... coli adhesion". Journal of Microbiological Methods. 184: 106201. doi:10.1016/j.mimet.2021.106201. PMID 33713725. Kimura K, ...
Uromodulin
A role in bacterial binding and sequestration is suggested by studies showing that Escherichia coli which express MS (mannose- ... "Structure of the decoy module of human glycoprotein 2 and uromodulin and its interaction with bacterial adhesin FimH". Nat. ...
Bacterial small RNA
5 prime ureB sRNA Aar small RNA, an sRNA produced by species of Acinetobacter Bacillus subtilis BSR sRNAs Escherichia coli sRNA ... Outer membrane proteins (OMPs) include porins and adhesins. Numerous sRNAs regulate the expression of OMPs. The porins OmpC and ... MicF in E. coli was found to regulate the expression of a key structural gene that makes up the outer membrane of the E. coli ... Hershberg R, Altuvia S, Margalit H (April 2003). "A survey of small RNA-encoding genes in Escherichia coli". Nucleic Acids ...
Pathogenicity island
2001 Structural and sequence diversity of the pathogenicity island of uropathogenic Escherichia coli which encodes the USP ... Pathogenicity islands carry genes encoding one or more virulence factors, including, but not limited to, adhesins, secretion ... mosomal regions coding for fimbriae and hemolysins occur in vivo and in vitro in various extraintestinal Escherichia coli iso- ...
Bacterial morphological plasticity
This mechanism has been described in bacteria such as Escherichia coli and Helicobacter pylori. Oxidative stress, nutrient ... with an increased number of adhesins participating in the interaction, making even harder the work for (PMN). The interaction ... Justice, Sheryl S.; Hunstad (2006). "Filamentation by Escherichia coli subverts innate defenses during urinary tract infection ... "The role of DNA base excision repair in filamentation in Escherichia coli K-12 adhered to epithelial HEp-2 cells". Antonie van ...
Chlamydia trachomatis
... is very similar to a homologous pair of proteins found in Escherichia coli (E. coli), though the reaction's speed is slower in ... When they come into contact with a new host cell, the elementary bodies bind to the cell via interaction between adhesins on ...
Figure - Fluoroquinolone-resistant Escherichia coli, Indonesia - Volume 11, Number 9-September 2005 - Emerging Infectious...
... putative adhesin-siderophore); bmaE (M fimbriae); gafD (G fimbriae); F17a (F17a fimbriae); clpG (CS31A adhesin); afaE8 ( ... Fluoroquinolone-resistant Escherichia coli, Indonesia Kuntaman Kuntaman*1, Endang Sri Lestari†1, Juliëtte A. Severin‡1, Irma M ... Box plot of relative mutation rate of 10 fluoroquinolone (FQ)-resistant (FQREC) and 10 FQ-sensitive (FQSEC) Escherichia coli. ... 3ExPEC: papEF (P fimbrial tip pilins); papG (P adhesin); papG alleles I, II, and III; sfaS (S fimbriae); focG (F1C fimbriae); ...
Blueberry: MedlinePlus Supplements
Guidelines for Prevention of Nosocomial Pneumonia
7 23 4 0 Escherichia coli 6 14 8 0 Serratia sp. 5 0 0 1 Proteus sp. 3 11 15 1 Citrobacter sp. 1 0 2 0 Acinetobacter ... Bacterial adherence: adhesin-receptor interactions mediating the attachment of bacteria to mucosal surfaces. J Infect Dis 1981; ... Adherence of Streptococcus pyogenes, Escherichia coli, and Pseudomonas aeruginosa to fibronectin-coated and uncoated epithelial ... Escherichia coli, Serratia marcescens, and Proteus sp. comprised 50% of the isolates from cultures of respiratory tract ...
Advanced Search Results - Public Health Image Library(PHIL)
Frontiers | Turn Up the Heat-Food and Clinical Escherichia coli Isolates Feature Two Transferrable Loci of Heat Resistance
... including Escherichia coli isolates from food production settings and clinical ESBL-producing E. coli isolates. Here, we ... coli isolates. Here, we describe the presence of two distinct LHR variants within a particularly heat resistant E. coli isolate ... The plasmid was highly transferable to other E. coli strains, including Shiga-toxin-producing strains, and conferred LHR- ... The plasmid was highly transferable to other E. coli strains, including Shiga-toxin-producing strains, and conferred LHR- ...
Bacterial adhesin - Wikipedia
The effectiveness of anti-adhesin antibodies is illustrated by studies with FimH, the adhesin of uropathogenic Escherichia coli ... Schembri MA, Klemm P (May 1998). "Heterobinary adhesins based on the Escherichia coli FimH fimbrial protein". Appl. Environ. ... August 1999). "X-ray structure of the FimC-FimH chaperone-adhesin complex from uropathogenic Escherichia coli". Science. 285 ( ... October 2011). "Type 1 fimbrial adhesin FimH elicits an immune response that enhances cell adhesion of Escherichia coli". ...
Acute Pyelonephritis: Practice Essentials, Background, Pathophysiology
Mannose-sensitive adhesins (usually type 1 fimbriae) are present on essentially all E coli. They contribute to colonization (eg ... Most bacterial data are derived from research with Escherichia coli, which accounts for 70-90% of uncomplicated UTIs and 21-54 ... A subset of E coli, the uropathogenic E coli (UPEC), also termed extraintestinal pathogenic E coli (ExPEC), accounts for most ... resistant Escherichia coli. Korean J Intern Med. 2016 Jan. 31 (1):145-55. [QxMD MEDLINE Link]. [Full Text]. ...
Guidelines for Prevention of Nosocomial Pneumonia
7 23 4 0 Escherichia coli 6 14 8 0 Serratia sp. 5 0 0 1 Proteus sp. 3 11 15 1 Citrobacter sp. 1 0 2 0 Acinetobacter ... Bacterial adherence: adhesin-receptor interactions mediating the attachment of bacteria to mucosal surfaces. J Infect Dis 1981; ... Adherence of Streptococcus pyogenes, Escherichia coli, and Pseudomonas aeruginosa to fibronectin-coated and uncoated epithelial ... Escherichia coli, Serratia marcescens, and Proteus sp. comprised 50% of the isolates from cultures of respiratory tract ...
Suppression subtractive hybridization identifies an autotransporter adhesin gene of E. coli IMT5155 specifically associated...
One of these loci contained a gene encoding a putative autotransporter adhesin. The open reading frame of the gene spans a ... Due to the nucleotide sequence similarity of 98% to a recently published adhesin-related gene, located on plasmid pAPEC-O1- ... In accordance with this hypothesis, the adhesin was found to be present not only in different phylogenetic groups of ... A specific antibody was raised against this protein and expression of the adhesin was shown under laboratory conditions. ...
Chemotaxis towards autoinducer 2 mediates autoaggregation in Escherichia coli | Nature Communications
... coli adhesin (antigen 43) or by curli fibres. Furthermore, AI-2-dependent autoaggregation enhances bacterial stress resistance ... Autoinducer 2 (AI-2) is the only known quorum-sensing molecule produced by Escherichia coli but its physiological role remains ... but its role in Escherichia coli is unknown. Here, Laganenka et al. show that chemotaxis towards self-produced AI-2 mediates ... Here we show that chemotaxis towards self-produced AI-2 can mediate collective behaviour-autoaggregation-of E. coli. ...
Publications | Molecular mechanisms of bacteria in infectious diseases and health
The O75X adhesin of uropathogenic Escherichia coli: receptor-active domains in the canine urinary tract and in vitro ... The O75X adhesin of uropathogenic Escherichia coli is a type IV collagen-binding protein. Mol Microbiol. 3:329-337. Blackwell ... Molecular structure of adhesin domains in Escherichia coli fimbriae. Invited review. Int. J. Med. Microbiol. 295:479-486. ... The gaf gene cluster of Escherichia coli expresses a full-size and a truncated soluble adhesin protein. J. Bacteriol. 183:512- ...
Ingegerd Adlerberth | University of Gothenburg
Adhesins of Escherichia coli associated with extra-intestinal pathogenicity confer binding to colonic epithelial cells. ... P fimbriae and other adhesins enhance intestinal persistence of Escherichia coli in early infancy. ... Decreased expression of mannose-specific adhesins by Escherichia coli in the colonic microflora of immunoglobulin A-deficient ... Reduced phase switch capacity and functional adhesin expression of type 1-fimbriated Escherichia coli from immunoglobulin A- ...
Professor Mark Schembri - UQ Researchers
Schembri, Mark A. and Klemm, Per (1998). Heterobinary adhesins based on the Escherichia coli FimH fimbrial protein. Applied and ... Fimbrial adhesins from extraintestinal Escherichia coli. Klemm, Per, Hancock, Viktoria and Schembri, Mark A. (2010). Fimbrial ... Valency conversion in the type 1 fimbrial adhesin of Escherichia coli. Sokurenko, E. V., Schembri, M. A., Trintchina, E., ... Molecular characterization of the Escherichia coli FimH adhesin. Schembri, M. A., Kjaergaard, K., Sokurenko, E. V. and Klemm, P ...
SCOPe 2.06: Species: Escherichia coli [TaxId: 562]
Superfamily b.2.3: Bacterial adhesins [49401] (7 families) *. Family b.2.3.5: F17c-type adhesin [89215] (3 proteins). ... Timeline for Species Escherichia coli [TaxId:562] from b.2.3.5 Fimbrial lectin GafD: *Species Escherichia coli [TaxId:562] from ... Species Escherichia coli [TaxId:562] from b.2.3.5 Fimbrial lectin GafD appears in SCOPe 2.05. *Species Escherichia coli [TaxId: ... PDB entry in Species: Escherichia coli [TaxId: 562]:. *Domain(s) for 1oio: *. Domain d1oioa_: 1oio A: [93069]. complexed with ...
SCOP 1.75: Domain d1oioa : 1oio A
Thieme E-Journals - Zeitschrift für Phytotherapie / Abstract
26 Schembri MA, Kjaergaard K, Sokurenko EV, Klemm P. Molecular characterization of the Escherichia coli FimH adhesin. J Infect ... Influence of berberine sulfate on synthesis and expression of pap fimbrial adhesin in uropathogenic Escherichia coli. ... Inhibition of uropathogenic Escherichia coli by cranberry juice: a new antiadherence assay. J Agric Food Chem 2005; 53: 8940- ... 23 Parkkinen J, Ristimäki A, Westerlund B. Binding of Escherichia coli S fimbriae to cultured human endothelial cells. Infect ...
Efficient immune responses against Intimin and EspB of enterohaemorragic Escherichia coli after intranasal vaccination using...
Escherichia coli secreted protein B) from enterohaemorragic E. coli co-administered with a pegylated derivative of the TLR2/6 ... Adhesins, Bacterial * Adjuvants, Immunologic * Antibodies, Bacterial * Bacterial Outer Membrane Proteins * EaeB protein, E coli ... Escherichia coli secreted protein B) from enterohaemorragic E. coli co-administered with a pegylated derivative of the TLR2/6 ... Efficient immune responses against Intimin and EspB of enterohaemorragic Escherichia coli after intranasal vaccination using ...
Figure - Fluoroquinolone-resistant Escherichia coli, Indonesia - Volume 11, Number 9-September 2005 - Emerging Infectious...
... putative adhesin-siderophore); bmaE (M fimbriae); gafD (G fimbriae); F17a (F17a fimbriae); clpG (CS31A adhesin); afaE8 ( ... Fluoroquinolone-resistant Escherichia coli, Indonesia Kuntaman Kuntaman*1, Endang Sri Lestari†1, Juliëtte A. Severin‡1, Irma M ... Box plot of relative mutation rate of 10 fluoroquinolone (FQ)-resistant (FQREC) and 10 FQ-sensitive (FQSEC) Escherichia coli. ... 3ExPEC: papEF (P fimbrial tip pilins); papG (P adhesin); papG alleles I, II, and III; sfaS (S fimbriae); focG (F1C fimbriae); ...
Analysis of the fim cluster of an avian O2 strain of Escherichia coli: serogroup-specific sites within fimA and nucleotide...
... coli and Klebsiella pneumoniae revealed that most differences were clustered in four well defined regions. A PCR assay, based ... Escherichia coli MT78, an avian pathogenic strain of serogroup O2, produces a variant form of type 1 fimbriae with distinct ... Gyimah J. E., Panigrahy B. Adhesin-receptor interactions mediating the attachment of pathogenic Escherichia coli to chicken ... Krogfelt K. A. Bacterial adhesion: genetics, biogenesis, and role in pathogenesis of fimbrial adhesins of Escherichia coli. Rev ...
Methicillin-Resistant Staphylococcus aureus Biofilms and Their Influence on Bacterial Adhesion and Cohesion
"The pgaABCD locus of Escherichia coli promotes the synthesis of a polysaccharide adhesin required for biofilm formation," ... by DNase I treatment of Gram-negative cells such as Pseudomonas aeruginosa and Escherichia coli, as well as Gram-positive cells ... D. Mack, W. Fischer, A. Krokotsch et al., "The intercellular adhesin involved in biofilm accumulation of Staphylococcus ... H. Rohde, E. C. Burandt, N. Siemssen et al., "Polysaccharide intercellular adhesin or protein factors in biofilm accumulation ...
Publications list | The James Hutton Institute
An investigation of the expression and adhesin function of H7 flagella in the interaction of Escherichia coli O157:H7 with ... Temporal and spatial patterns of bovine Escherichia coli O157 prevalence and comparison of temporal changes in the patterns of ... Spread of E. coli O157 infection among Scottish cattle farms: Stochastic models and model selection. Epidemics. 2:11-20. ... phage types associated with bovine shedding and human E. coli O157 cases in Scotland between 1998-2000 and 2002-2004. BMC ...
Grand Challenges Africa | The AAS
Project - Anti-adhesins with therapeutic potential for enteroaggregative Escherichia coli diarrhoea. Iruka N Okeke is a ... Project - Anti-adhesins with therapeutic potential for enteroaggregative Escherichia coli diarrhoea. The relatively unknown and ... Escherichia coli and Klebsiella pneumoniae in pediatric and maternal populations within Uganda. ... understudied enteric pathogen enteroaggregative Escherichia coli (EAEC) is an important cause of infantile diarrhoea in Nigeria ...
Medicine
- Table of Contents
Role of Escherichia coli O157:H7 virulence factors in colonization at the bovine terminal rectal mucosa
Outer membrane protein A (OmpA) of extraintestinal pathogenic Escherichia coli | BMC Research Notes | Full Text
... are responsible for host diseases such as Neonatal Meningitis Escherichia coli (NMEC), the second-leading cause of neonatal ... Although OmpA is present in virtually all E. coli, differences in its amino acid residues have yet to be surveyed in ExPEC. ... bacterial meningitis, Avian Pathogenic E. coli (APEC), a cause of extraintestinal disease in poultry, and Uropathogenic E. coli ... of chromosomal history and polymorphism patterns in a virulence factor has precedence as polymorphisms in the adhesin FimH, a ...
Adhesins, Bacterial | Profiles RNS
Adhesins, Escherichia coli. publications Timeline , Most Recent This graph shows the total number of publications written about ... What is sometimes called polymeric adhesin (BIOFILMS) is distinct from protein adhesin. ... "Adhesins, Bacterial" by people in this website by year, and whether "Adhesins, Bacterial" was a major or minor topic of these ... "Adhesins, Bacterial" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical ...
Blood group antigens. Medical search
Adhesins, Escherichia coli. Thin, filamentous protein structures, including proteinaceous capsular antigens (fimbrial antigens ... ProtozoanAdhesins, Escherichia coliBase SequencePlasmodium vivaxAllelesHLA AntigensAntigens, Polyomavirus TransformingColonic ... ProtozoanAdhesins, Escherichia coliHLA AntigensAntigens, Polyomavirus TransformingAntibodiesRhamnoseAntigens, FungalAntigens, ... Ab113153 is a full length protein produced in Escherichia coli ... This protein is the basis of the ABO blood group system. The ...
Roberto Carlos Carrillo Torres - Research Output
- Universidad de Sonora
EnterotoxigenicO157:H7Pathogenic Escherichia coliETECProteinsFimbriaeIsolatesGenesUropathogenic EscherichiaUPECProteinIntestinalToxinsBiofilmFimHCharacterizationPiliPutative adhesinReceptorsEnteroaggregativeAbstractPathogenicityAdherenceStaphylococcusAntimicrobial resistanceAntigenStrainLectinPathogensInfectionSpeciesGeneKlebsiellaAutotransporter adhesinOperonPhylogeneticEnterobacteriaceaeAdhesionShigaMutantsGenomeAmino acidStreptococcusBacteriumAntigensSalmonella
Enterotoxigenic10
- Enterotoxigenic Escherichia coli (ETEC) is closely associated with diarrhoea in children in resource-limited countries and of travellers' diarrhoea. (researchsquare.com)
- Enterotoxigenic Escherichia coli (ETEC) is a major cause of diarrhoea in children in resource-limited countries and of travellers' diarrhoea 1 . (researchsquare.com)
- Structural and functional insight into the carbohydrate receptor binding of F4 fimbriae-producing enterotoxigenic Escherichia coli. (ac.be)
- Enterotoxigenic Escherichia coli (ETEC) strains are important causes of intestinal disease in humans and lead to severe production losses in animal farming. (ac.be)
- Enterotoxigenic Escherichia coli (ETEC) expressing the colonization pili CFA/I are common causes of diarrhoeal infections in humans. (edu.sa)
- F4-positive enterotoxigenic Escherichia coli (ETEC) strains are a frequent cause of porcine post-weaning diarrhea. (european-biotechnology.com)
- The interactions between enterotoxigenic Escherichia coli (ETEC), the main pathogenic agent of travelers' diarrhea, intestinal mucus and human gut microbiota remain unknown. (nature.com)
- However, our studies show we can obtain elevated Th2 cell (IL-4- and IL-13-dependent) immune responses, followed by a delayed onset of Th1 cells to colonization factor antigen I (CFA/I), from human enterotoxigenic Escherichia coli (ETEC). (ufl.edu)
- Post-Weaning Diarrhea (PWD) due to Enterotoxigenic E. coli (ETEC) in pigs is a worldwide economically important disease associated with abnormal fecal consistency and higher use of antimicrobials. (biomedres.us)
- Enterotoxigenic E. coli (ETEC) is regarded the most important cause of PWD. (biomedres.us)
O157:H714
- Detection of Escherichia coli O157:H7 virulence genes in isolates from beef, pork, water, human and animal species in the northwest province, South Africa: Public health implications. (uptc.edu.co)
- Molecular characteristics and genotypic diversity of enterohaemorrhagic Escherichia coli O157:H7 isolates in Gauteng region, South Africa. (uptc.edu.co)
- Escherichia coli O157:H7 str. (weizmann.ac.il)
- However, a sub-group of non-STEC have increasingly been associated with serious illness called Heamolytic Uremic Syndrome (kidney failure) that has hitherto being mainly restricted to the E. coli O157:H7 serotype. (chestervetclinic.com)
- The E. coli O157:H7 then produces a protein called intimin (encoded by eae gene) and Tir (encoded by tir gene) that facilitates attachment to the epithelial cell surface. (chestervetclinic.com)
- E. coli O157:H7 also forms a tube (Type III secretion system) to enable proteins to be transferred from the bacteria into the host. (chestervetclinic.com)
- Although there are 25 proteins transferred from E. coli O157:H7 into the epithelial cell the most significant is the Shiga like toxin (encoded by stx gene). (chestervetclinic.com)
- E. coli O157:H7 also produces a heamolysin that dissolves blood cells thereby contributing to the virulence of the pathogen. (chestervetclinic.com)
- When E. coli O157:H7 cells are ingested, the infected person will start developing the common characteristics of foodborne illness such as fever, nausea, stomach cramps and diarrhea. (chestervetclinic.com)
- Escherichia coli O157:H7 carried on plant surfaces, including alfalfa sprouts, has been implicated in food poisoning and outbreaks of disease in the United States. (utmb.edu)
- Several E. coli O157:H7 surface proteins are thought to be important for adhesion and/or biofilm formation. (utmb.edu)
- In contrast, deletion of one or more of these genes in a strain of E. coli O157:H7 did not affect its ability to bind to alfalfa. (utmb.edu)
- In contrast, the E. coli O157:H7 ompA and tdcA ompA mutant strains were only slightly affected in adhesion to Caco-2 cells and during biofilm formation. (utmb.edu)
- These findings suggest that some adhesins alone are sufficient to promote binding to alfalfa and that they may exist in E. coli O157:H7 as redundant systems, allowing it to compensate for the loss of one or more of these systems. (utmb.edu)
Pathogenic Escherichia coli4
- Gyimah J. E. , Panigrahy B. Adhesin-receptor interactions mediating the attachment of pathogenic Escherichia coli to chicken tracheal epithelium. (microbiologyresearch.org)
- Sekizaki T. , Ito H. , Asawa T. , Nonomura I. DNA sequence of type 1 fimbrin, Fpull, gene from a chicken pathogenic Escherichia coli serotype O78. (microbiologyresearch.org)
- Members of the Extraintestinal Pathogenic Escherichia coli (ExPEC) pathotype are adapted for an extraintestinal lifestyle. (biomedcentral.com)
- Phenotypic and genotypic analysis of pathogenic Escherichia coli virulence genes recovered from Riyadh, Saudi Arabia. (uptc.edu.co)
ETEC4
- This study aims to investigate the change of ileal mucosal microbiome and ileal protein expression as well as their correlation in pigs by E. coli K88 (ETEC). (researchsquare.com)
- ETEC adheres to the intestinal epithelial cells (IEC) in the jejunum and ileum through adhesins interacting with specific receptors and secrets enterotoxins to cause perturbation of hydroelectrolytic secretions resulting in a rapid onset of secretory diarrhoea leading to dehydration 5 . (researchsquare.com)
- A range of fimbrial adhesins in ETEC strains determines host and tissue tropism. (ac.be)
- The ETEC pathotype is typically characterized by the presence of fimbrial adhesins, which mediate attachment to porcine intestinal enterocytes, and enterotoxins, which disrupt fluid homeostasis in the small intestine. (biomedres.us)
Proteins8
- At the end of each fimbria are special proteins called adhesins. (web.app)
- Recombinant CspA (rCspA) and CspB (rCspB) proteins were generated in Escherichia coli and used to produce pAbs. (microbiologyresearch.org)
- Localized adherence by enteropathogenic escherichia coli is an inducible phenotype associated with the expression of new outer membrane proteins. (web.app)
- Eiec are highly invasive, and they use adhesin proteins to bind to and enter intestinal cells. (web.app)
- Although each family of adhesion proteins is generally associated with a specific human disease, the Dr family from Escherichia coli is a notable exception, as its members are associated with both diarrheal and urinary tract infections. (pasteur.fr)
- Trimeric autotransporter adhesins (TAAs) are modular, highly repetitive surface proteins that mediate adhesion to host cells in a broad range of Gram-negative pathogens. (mpg.de)
- Morgan Dasovich, Morgan Q. Beckett, Scott Bailey, Shao-En Ong, Marc M. Greenberg, and Anthony K. L. Leung (2021) Identifying Poly(ADP-ribose)-Binding Proteins with Photoaffinity-Based Proteomics. (jhu.edu)
- We also mined a series of novel promising virulence-associated factors in our study compared with those in previous reports, such as some moonlighting adhesins, transporters, lipoate-protein ligase, and ribonuclease and several hypothetical proteins with conserved functional domains, deserving further research. (hindawi.com)
Fimbriae22
- The plasmid was highly transferable to other E. coli strains, including Shiga-toxin-producing strains, and conferred LHR-dependent heat resistance as well as type 3 fimbriae-dependent biofilm formation capabilities. (frontiersin.org)
- Most fimbria of gram-negative bacteria function as adhesins, but in many cases it is a minor subunit protein at the tip of the fimbriae that is the actual adhesin. (wikipedia.org)
- The response regulator RcsB activates expression of Mat fimbriae in meningitic Escherichia coli . (helsinki.fi)
- The fimbriae activator MatA switches off motility in Escherichia coli by repression of the flagellar master operon flhDC . (helsinki.fi)
- Mat fimbriae promote biofilm formation by meningitis-associated Escherichia coli . (helsinki.fi)
- 2009. The SfaX(II) protein from newborn meningitis E. coli is involved in regulation of motility and type 1 fimbriae expression. (helsinki.fi)
- Escherichia coli MT78, an avian pathogenic strain of serogroup O2, produces a variant form of type 1 fimbriae with distinct antigenic properties and apparent mol. (microbiologyresearch.org)
- Blomfield I. C. , McClain M. S. , Eisenstein B. I. Type 1 fimbriae mutants of Escherichia coli K12: characterization of recognized afimbriate strains and construction of new fim deletion mutants. (microbiologyresearch.org)
- Most fimbriae (FIMBRIAE, BACTERIAL) of gram-negative bacteria function as adhesins, but in many cases it is a minor subunit protein at the tip of the fimbriae that is the actual adhesin. (jefferson.edu)
- Flagella are long, helical filaments made of a single type of… The importance of p and type 1 fimbriae for the persistence of escherichia coli in the human gut volume 108 issue 3 k. tullus, i. kühn, i. (web.app)
- Fimbriae are av B Wullt · 2001 · Citerat av 93 - Bacterial adhesion to the bladder mucosa is a critical step for the establishment of Escherichia coli bacteriuria. (web.app)
- The specific type of adhesin varies by type of bacteria, but regardless of Fimbriae are a major bacterial virulence factor (something that helps a bacterium cause disease). (web.app)
- Fimbriae, Bacterial Research Article Altered Regulation of the Diguanylate Cyclase YaiC Reduces Production of Type 1 Fimbriae in a Pst Mutant of Uropathogenic Escherichia coli CFT073 (a) Bacteria containing fimbriae are called fimbriate bacteria. (web.app)
- A hemagglutination-based assay with E. coli expressing mutant F4ad fimbriae confirmed the elucidated co-complex structure. (ac.be)
- Orally administered F4 fimbriae or FaeG, the major subunit and adhesin of F4, induce a protective mucosal immune response in F4 receptor-positive piglets. (european-biotechnology.com)
- The genes with the highest circulation were Stx1, Stx2 coding for toxins, Saa for adhesins, ehxA for enterohemolysin , eaeA for intimin, and IpfA for fimbriae. (uptc.edu.co)
- Huvudskillnad - APA vs Harvard Referensreferenser är en viktig aktivitet som bör vara exakt känd av akademiska forskare Skillnaden mellan Pili och Fimbriae. (web.app)
- Published Biofilm formation is mediated by Csu pili, assembled via the "archaic" suggesting that archaic pili use tip-fingers to detect and bind to hydrophobic cavities in escherichia-coli, pilus, biogenesis, yersinia-pestis, psa fimbriae, adhesin, fimh, The shaft of the type 1 fimbriae regulates an external force to match the FimH catch pneumoniae-A Comparison between Helix‐like and Open Coil‐like Pili. (web.app)
- Pili vs Fimbriae Pili en fimbriae staan bekend als filamenteuze aanhangsels, die voornamelijk worden gebruikt voor adhesie. (web.app)
- De termen Pili en fimbriae Pili vs. (web.app)
- Les mots pili et fimbriae sont souvent interchangeables, mais dans le domaine de la bactériologie, on appelle plutôt « fimbria » (fimbriae au pluriel) les pili de petite taille qui caractérisent de nombreuses bactéries (pili est le pluriel de pilus, qui en latin désigne le poil ou le cheveu). (web.app)
- Other fimbriae such as F5 (K99), F6 (987P) and F41 rarely occur in E. coli isolates from PWD [9-13]. (biomedres.us)
Isolates7
- A recently identified locus of heat resistance (LHR) has been shown to be present in and confer heat resistance to a variety of Enterobacteriaceae , including Escherichia coli isolates from food production settings and clinical ESBL-producing E. coli isolates. (frontiersin.org)
- Identification of a Latin American-specific BabA adhesin variant through whole genome sequencing of Helicobacter pylori patient isolates from Nicaragua. (cdc.gov)
- This study determined the frequency of diarrhoeagenic E. coli isolates collected from children with acute diarrhoea (n = 50) and a control group (n = 50) at an Iranian referral paediatric centre during a 1-year period. (who.int)
- Characterization of AfaE adhesins produced by extraintestinal and intestinal human Escherichia coli isolates: PCR assays for detection of Afa adhesins that do or do not recognize Dr blood group antigens. (childrensmercy.org)
- Genomic and phylogenetic characteristics of E. coli pneumonia isolates from critically ill patients indicate that they belong to the extraintestinal pathogenic E. coli pathovar but have distinguishable lung-specific traits. (cdc.gov)
- we then hybridized isolates with DNA probes and identified enteropathogenic E. coli (EPEC), enteroaggregative E. coli (EAEC), and diffusely adherent E. coli (DAEC). (cdc.gov)
- We conducted a case-control study on E. coli isolates that were categorized as EPEC, EAEC, and DAEC by adherence tests and DNA probing. (cdc.gov)
Genes15
- Whereas most genes were well conserved relative to fim genes previously described, comparison of the fimA gene from strain MT78 with homologous sequences from other strains of E. coli and Klebsiella pneumoniae revealed that most differences were clustered in four well defined regions. (microbiologyresearch.org)
- In O104:H4 strains, the missing central portion of the LEE locus was replaced by a pathogenicity island carrying the aidA (adhesin involved in diffuse adherence) gene and antibiotic resistance genes commonly carried on plasmids. (pacb.com)
- Enteroaggregative E. coli-specific virulence genes and European outbreak O104:H4-specific stx2-encoding Escherichia P13374 or Escherichia TL-2011c bacteriophages were missing in some of the O104:H4 genome sequences available from public databases. (pacb.com)
- In the present work a new set of escherichia coli k12 genes conferring. (web.app)
- Genes and expression of virulence factors in Escherichia coli isolated from production animals. (uptc.edu.co)
- The present review aims to describe virulence factors and genes encoding them in E. coli strains isolated from production animals and food products. (uptc.edu.co)
- Finally, E. coli has been the workhorse of molecular biology and provided a wealth of information on the function of genes and how these can be manipulated to produce valuable products such as insulin used to treat diabetes. (chestervetclinic.com)
- Indeed, it is the acquisition of genes that gave rise to a sub-set of E. coli termed pathogenic E. coli . (chestervetclinic.com)
- Escherichia coli harboring shiga toxin(s) genes collectively fall with the STEC group and encompasses over 200 different serotypes (Couturier et al. . (chestervetclinic.com)
- Therefore, we examined whether mutations in several genes encoding potential adhesins and regulators of adherence have an effect on bacterial binding to plants and also examined the role of these genes during adhesion to Caco-2 cells and during biofilm formation on plastic in vitro. (utmb.edu)
- The genes tested included those encoding adhesins (cah, aidA1, and ompA) and mediators of hyperadherence (tdcA,yidE, waaI, and cadA) and those associated with fimbria formation (csgA, csgD, and lpfD2). (utmb.edu)
- The introduction of some of these genes (cah, aidA1, and csg loci) into an E. coli K-12 strain markedly increased its ability to bind to alfalfa sprouts and seed coats. (utmb.edu)
- Two papers in Nature report synthetic biological circuits , a genetic toggle switch and a biological clock, by combining genes within E. coli cells. (biolaw.one)
- Prevalence of urovirulence genes in Escherichia coli strains isolated from the feces of dogs (n = 61), their owners (61), and non-dog owners (30). (avma.org)
- The quantitative level of resistance was >256 mg/L. PFGE showed a dominance of genotype A. PCR revealed more than half of the MRSA strains, while only 1/3 of the MSSA ones to possess four adhesin genes in various combinations. (otka-palyazat.hu)
Uropathogenic Escherichia5
- The effectiveness of anti-adhesin antibodies is illustrated by studies with FimH, the adhesin of uropathogenic Escherichia coli (UPEC). (wikipedia.org)
- Host‐specificity of uropathogenic Escherichia coli depends on differences in binding specificity to Gal alpha 1‐4Gal‐containing isoreceptors. (google.com.co)
- Isolation of trna from uropathogenic escherichia coli. (web.app)
- Inhibition of uropathogenic escherichia coli by cranberry. (web.app)
- The aim of this study is that, extraction of trna from uropathogenic escherichia coli then detect the presence of such molecules after extraction and measure the purity of the trna extract solutions. (web.app)
UPEC5
- Im Rahmen einer systematischen Untersuchung traditionell gegen UTI eingesetzter Arzneipflanzen wurde innerhalb einer In-vitro-Teilstudie untersucht, inwieweit solche Drogen potenzielle antiadhäsive Effekte gegenüber uropathogenen Escherichia coli (UPEC) und deren Erkennungs- und Anheftungsstrategien an humane Blasenzellen aufweisen. (thieme-connect.com)
- Within a systematic project of medicinal plants, used traditionally against uncomplicated urinary tract infections (UTI), this study investigated anti-adhesive effects of plant extracts against uropathogenic E. coli (UPEC). (thieme-connect.com)
- Extraintestinal Pathogenic E. coli (ExPEC), are responsible for host diseases such as Neonatal Meningitis Escherichia coli (NMEC), the second-leading cause of neonatal bacterial meningitis, Avian Pathogenic E. coli (APEC), a cause of extraintestinal disease in poultry, and Uropathogenic E. coli (UPEC), the most common cause of urinary tract infections. (biomedcentral.com)
- With SadA from Salmonella enterica, EhaG from enteropathogenic Escherichia coli (EHEC), and UpaG from uropathogenic E. coli (UPEC), we present three representative structures of a complex adhesin that occur in a conserved genomic context in Enterobacteria and is essential in the infection process of uropathogenic E. coli. (mpg.de)
- The E. coli uropathogenic strains (UPEC) are responsible for about 90% of uncomplicated cystitis. (infodeakos.com)
Protein16
- In gram-positive bacteria, a protein or polysaccharide surface layer serves as the specific adhesin. (wikipedia.org)
- The bacterial adhesion consists primarily of an intramembranous structural protein which provides a scaffold upon which several extracellular adhesins may be attached. (wikipedia.org)
- The phylogenetic trees were calculated with the Neighbor-Joining-Algorithm on the basis of a ClustalW multiple alignment of 24 protein sequences from known adhesins of the autotransporter family including AatA. (biomedcentral.com)
- A major determinant of autoaggregation in E. coli is antigen 43 (Ag43), the abundant outer membrane protein that belongs to the autotransporter family and is secreted via the type V secretion system 4 . (nature.com)
- Two distinct regions in the model protein Peb1 are critical for its heterologous transport out of Escherichia coli . (helsinki.fi)
- Mucosal vaccine formulations based on purified recombinant C280 gamma-Intimin and EspB (Escherichia coli secreted protein B) from enterohaemorragic E. coli co-administered with a pegylated derivative of the TLR2/6 agonist MALP-2 (macrophage-activating lipopeptide) as adjuvant were evaluated in BALB/c mice. (nih.gov)
- What is sometimes called polymeric adhesin (BIOFILMS) is distinct from protein adhesin. (jefferson.edu)
- In an initial step, Mycobacterum tuberculosis (Mtb) binds alveolar MOs through mycobacterial adhesins or opsonins that interact with a variety of receptors, including CR1, CR3, and CR4, IgGFc, surfactant A protein and C-type lectins [ 3 ]. (biomedcentral.com)
- A Protein Synthesis in Prokaryotes: The mechanism of protein synthesis has been thoroughly investigated in Escherichia coli. (speedweb.shop)
- The process of protein synthesis in E. coli involves the following steps: 1. (speedweb.shop)
- Current work has adapted the rodent EAE model to test whether our tolerogen vaccine delivery platform, the reovirus adhesin, protein sigma 1 (pσ1), can improve mucosal auto-antigen uptake. (ufl.edu)
- It consists of a thick peptidoglycan layer, teichoic acid, protein-A, and cell surface adhesins (e.g. clumping factor). (notesmed.com)
- protein_coding" "AAC73318","yafS","Escherichia coli","putative S-adenosyl-L-methionine-dependent methyltransferase [Ensembl]. (ntu.edu.sg)
- protein_coding" "AAC73721","dpiA","Escherichia coli","response regulator in two-component regulatory system with CitA [Ensembl]. (ntu.edu.sg)
- protein_coding" "AAC74828","ynjF","Escherichia coli","CDP-alcohol phosphatidyltransferase family inner membrane protein [Ensembl]. (ntu.edu.sg)
- Recombinant protein production was carried out with E. coli BL21 (DE3) as the host [61]. (pdgfrsignals.com)
Intestinal3
- According to the source of the infection, pathogenic E. coli can be classified as intestinal (diarrheagenic) and extraintestinal (ExPEC). (opensourcebiology.eu)
- When E. coli O157 is ingested it can survive the acid of the stomach and eventually reaches the colon where it binds to a receptor on the epithelial cells of the gastro-intestinal tract. (chestervetclinic.com)
- Several alternative strategies have been explored to increase intestinal health and decrease incidence of PWD due to E. coli in post-weaned piglets [21-23]. (biomedres.us)
Toxins2
- The initial step of numerous prokaryotic and viral infections consists in the binding of adhesins of pathogens and toxins secreted by bacteria to the cell surface of susceptible target cells of the host, whereupon the actual disease is triggered. (internet1.de)
- The mechanisms of evasion from host immunity and action of adhesins, toxins and other virulence factors are analyzed in detail using prototypic examples of human and animal pathogens. (vscht.cz)
Biofilm6
- Autoinducer 2 (AI-2) is the only known quorum-sensing molecule produced by Escherichia coli but its physiological role remains elusive, although it is known to regulate biofilm formation and virulence in other bacterial species. (nature.com)
- The Gram-negative bacterium Escherichia coli is one of the model organisms for studying both cell aggregation and biofilm formation. (nature.com)
- in biofilm development lead to the finding that conjugative pili might act as cell adhesin interconnecting and stabilizing biofilm structure. (kenyon.edu)
- Second, deleting all innate determinants of biofilm formation, including cell surface structures and displaying artificial adhesins (e.g. nanobodies) on the envelope of the resulting naked strain. (vdl-lab.com)
- The gene icaADBC has been found to code for both the polysaccharide capsule and the polysaccharide intracellular adhesin used in biofilm formation. (joerggraflab.com)
- LapG mediates biofilm dispersal in Vibrio fischeri by controlling maintenance of the VCBS-containing adhesin LapV. (luc.edu)
FimH1
- The best characterized bacterial adhesin is the type 1 fimbrial FimH adhesin. (wikipedia.org)
Characterization1
- Pdf characterization of colicinogenic escherichia coli. (web.app)
Pili3
- Suwanichkul A. , Panigrahy B. , Wagner R. M. Antigenic relatedness and partial amino acid sequences of pili of Escherichia coli serotypes 01, 02, and 078 pathogenic to poultry. (microbiologyresearch.org)
- Hull R. A. , Gill R. E. , Hsu P. , Minshew B. H. , Falkow S. Construction and expression of recombinant plasmids encoding type 1 or D-mannose-resistant pili from a urinary tract infection Escherichia coli isolate. (microbiologyresearch.org)
- RESULT: We observed staining of endometrial glands, spiral arterioles, and myometrial arteries with Dr adhesin (pili) and anti-DAF (SCR-3) IgG, and found variation in distribution and amount of Dr ligands in different individuals. (okstate.edu)
Putative adhesin1
- The gene product encoded by AidA, an auto-secreted putative adhesin, has been found to be up-regulated during infection, using a gene discovery method known as In-vivo Induced Antigen Technology (IVIAT). (uwyo.edu)
Receptors2
- 3 Edén CS, Leffler H. Glycosphingolipids of human urinary tract epithelial cells as possible receptors for adhering Escherichia coli bacteria . (thieme-connect.com)
- For example, E. coli utilizes them to get attached to the mannose receptors. (web.app)
Enteroaggregative2
- Enteroaggregative escherichia coli eaec is a heterogeneous emerging enteric pathogen identified during the 1980s when eaec strains were isolated from cases of acute and persistent diarrhea among infants in developing countries as well as travelers diarrhea. (web.app)
- patients and controls did not differ in the rate of isolation of diffusely adhering E. coli (DAEC) (31% and 32%, respectively) or enteroaggregative E. coli (EAEC) (10% and 8%, respectively). (cdc.gov)
Abstract1
- ABSTRACT Diarrhoeagenic Escherichia coli can be considered as the most important etiologic agents of diarrhoea in the Islamic Republic of Iran, particularly in children. (who.int)
Pathogenicity1
- Escherichia coli and how the O104:H4 serotype has changed our understanding of pathogenicity of E. coli . (chestervetclinic.com)
Adherence3
- Adhesins are cell-surface components or appendages of bacteria that facilitate adhesion or adherence to other cells or to surfaces, usually in the host they are infecting or living in. (wikipedia.org)
- To effectively achieve adherence to host surfaces, many bacteria produce multiple adherence factors called adhesins. (wikipedia.org)
- HEp-2-adherent Escherichia coli strains that show localized adherence (LA), aggregative adherence (AA), diffuse adherence (DA), and localized adherence-like (LAL) patterns have been implicated as diarrheal pathogens (1) . (cdc.gov)
Staphylococcus3
- Adhesive polypeptides of Staphylococcus aureus identified using a novel secretion library technique in Escherichia coli . (helsinki.fi)
- Dairy cows often develop different degrees of endometritis after calving and this is attributed to pathogenic bacterial infections such as by Escherichia coli and Staphylococcus aureus . (biomedcentral.com)
- The results of UV-visible and energy-dispersive spectrometry confirmed their important carbon polymerization structures and the activity of the nitro group, which had an evident inhibitory effect on P. gingivalis , but almost no effect on other bacteria, including Escherichia coli , Staphylococcus aureus and Prevotella nigrescens . (dovepress.com)
Antimicrobial resistance3
- However, it has still to be shown in practice that the vaccine that fights of pathogenic E.coli bacteria is effective and safe and can help to fight the global health threat of antimicrobial resistance (AMR). (european-biotechnology.com)
- Virulence determinants and antimicrobial resistance of E. coli isolated from bovine clinical mastitis in some selected dairy farms of Bangladesh. (uptc.edu.co)
- The disease is currently controlled using antimicrobials, although the emergence of antimicrobial resistance in E. coli strains isolated from cases of PWD urges the need for alternative control measures [14-18]. (biomedres.us)
Antigen3
- These effects are observed regardless whether cell-cell interactions under particular growth conditions are mediated by the major E. coli adhesin (antigen 43) or by curli fibres. (nature.com)
- by immunoblot, it was found that the plasma membranes of phosphate-deprived bacilli express the adhesins PstS-1, LpqH, LprG, and the APA antigen. (biomedcentral.com)
- we found that together with PstS-1, the membranes of Pi-deprived mycobacteria express the mycobacterial adhesins LpqH, LprG, and the APA antigen, glycoproteins that are not directly involved in phosphate regulation. (biomedcentral.com)
Strain3
- The intimin (eae) gene, involved in the attaching-effacing phenotype of diarrheagenic E. coli, was not found in either strain. (pacb.com)
- A hypothesis on strain evolution and pathogenic potential of various H-serotypes of E. coli O104 strains is proposed. (pacb.com)
- The E. coli strain DH5α was used as a standard cloning host [59]. (pdgfrsignals.com)
Lectin1
- 4b4p (Br: 13) - Crystal Structure of the Lectin Domain of F18 Fimbrial Adhesin Fedf. (atomistry.com)
Pathogens3
- Many bacterial pathogens are able to express an array of different adhesins. (wikipedia.org)
- For example, E. coli produces Vitamin K that we use as part of cell repair (blood clotting) and are far more effective probiotics than lactic acid bacteria in out-competing would be pathogens such as Salmonella and Clostridium difficile . (chestervetclinic.com)
- Its presence indicates the potential presence of virulent pathogens such as Salmonella , E. coli O157 and Shigella , amongst others. (chestervetclinic.com)
Infection5
- Expression of these adhesins at different phases during infection play the most important role in adhesion based virulence. (wikipedia.org)
- This has led to the exploration of adhesin activity interruption as a method of bacterial infection treatment. (wikipedia.org)
- Additionally, Adhesins are attractive vaccine candidates because they are often essential to infection and are surface-located, making them readily accessible to antibodies. (wikipedia.org)
- Enteroinvasive escherichia coli eiec is a type of pathogenic bacteria whose infection causes a syndrome that is identical to shigellosis, with profuse diarrhea and high fever. (web.app)
- Infection of the bovine en. (biomedcentral.com)
Species1
- Bacterial adhesins provide species and tissue tropism. (wikipedia.org)
Gene2
- Prevalence and virulence gene profiles of Escherichia coli O157 from cattle slaughtered in Buea, Cameroon. (uptc.edu.co)
- In a prospective, nationwide study in France of Escherichia coli responsible for pneumonia in patients receiving mechanical ventilation, we determined E. coli antimicrobial susceptibility, phylotype, O-type, and virulence factor gene content. (cdc.gov)
Klebsiella1
- Predictability of phenotype in relation to common β-lactam resistance mechanisms in Escherichia coli and Klebsiella pneumoniae. (cdc.gov)
Autotransporter adhesin1
- 4 'Suzuki, A.' 5 'Linke, D.' 6 'Lupas, A.N.' 7 'Hori, K.' 8 # _citation.id primary _citation.title ;Structural Basis for Toughness and Flexibility in the C-terminal Passenger Domain of an Acinetobacter Trimeric Autotransporter Adhesin. (rcsb.org)
Operon2
- Neely MN, Dell CL, Olson ER: Roles of LysP and CadC in mediating the lysine requirement for acid induction of the Escherichia coli cad operon. (cetp-signal.com)
- Jacob and Monod postulate cellular regulation by molecular networks from their study of the lac operon in E. coli and envisioned the ability to assemble new systems from molecular components. (biolaw.one)
Phylogenetic1
- Phylogenetic Group -Associated Differences in Regulation of the Common Colonization Factor Mat Fimbria in Escherichia coli . (helsinki.fi)
Enterobacteriaceae1
- According to the World Health Organization, Enterobacteriaceae , including Escherichia coli , are among the critical priority antibiotic-resistant bacteria ( 7 ). (cdc.gov)
Adhesion5
- Adhesion and bacterial adhesins are also a potential target for prophylaxis or treatment of bacterial infections. (wikipedia.org)
- This adhesin is responsible for D-mannose sensitive adhesion. (wikipedia.org)
- This basic structure is conserved across type 1 fimbrial adhesins though recent studies have shown that in vitro induced mutations can lead to the addition of C-terminal domain specificity resulting in a bacterial adhesion with dual bending sites and related binding phenotypes. (wikipedia.org)
- Although their sizes may differ by more than one order of magnitude, they all follow the same basic head-stalk-anchor architecture, where the head mediates adhesion and autoagglutination, the stalk projects the head from the bacterial surface, and the anchor provides the export function and attaches the adhesin to the bacterial outer membrane after export is complete. (mpg.de)
- Moreover, the fungal adhesins playing a role in adhesion to host have been only explored in yeasts. (pasteur.fr)
Shiga3
- Genome sequencing and comparative genomics provides insights on the evolutionary dynamics and pathogenic potential of different H-serotypes of Shiga toxin-producing Escherichia coli O104. (pacb.com)
- Various H-serotypes of the Shiga toxin-producing Escherichia coli (STEC) O104, including H4, H7, H21, and H¯, have been associated with sporadic cases of illness and have caused food-borne outbreaks globally. (pacb.com)
- The outbreak of Shiga Toxin producing Escherichia coli O104:H4 linked to bean sprouts led to over 3800 confirmed cases of illness that included more than 823 cases of Heamolytic Uremic Syndrome (HUS) and 44 deaths (Frank et al . (chestervetclinic.com)
Mutants1
- Bruni CB, Colantuoni V, Sbordone L, Cortese R, Blasi F: Biochemical and regulatory properties of Escherichia coli K-12 hisT mutants. (cetp-signal.com)
Genome1
- Researchers report the production of a new synthetic (possibly artificial ) form of viable life , a variant of the bacteria Escherichia coli , by reducing the natural number of 64 codons in the bacterial genome to 59 codons instead, in order to encode 20 amino acids . (biolaw.one)
Amino acid1
- Although OmpA is present in virtually all E. coli , differences in its amino acid residues have yet to be surveyed in ExPEC. (biomedcentral.com)
Streptococcus1
- Stubbs HE , Bensing BA , Yamakawa I , Sharma P , Yu H , Chen X , Sullam PM , Iverson TM , Tandem sialoglycan-binding modules in a Streptococcus sanguinis serine-rich repeat adhesin create target dependent avidity effects Journal of Biological Chemistry. (vanderbilt.edu)
Bacterium2
- Numerous studies have shown that inhibiting a single adhesin in this coordinated effort can often be enough to make a pathogenic bacterium non-virulent. (wikipedia.org)
- One of the main features that makes E. coli easy to manipulate in genetic engineering is the ease with which genetic material can be introduced and alter the physiological traits of the bacterium. (chestervetclinic.com)
Antigens1
- Dho-Moulin M. , van den Bosch J. F. , Girardeau J. P. , Bree A. , Lafont J. P. Surface antigens from Escherichia coli 02 and 078 strains of avian origin. (microbiologyresearch.org)
Salmonella1
- Escherichia coli , # Neisseria meningitidis , ° Haemophilus influenzae , + Yersinia enterocolitica , ' Moraxella catarrhalis , ´´ Helicobacter pylori , $ Xylella fastidiosa , ** Salmonella Typhimurium, and & Bordetella pertussis . (biomedcentral.com)