A carbon-nitrogen ligase. During purine ribonucleotide biosynthesis, this enzyme catalyzes the synthesis of adenylosuccinate from GTP; IMP; and aspartate with the formation of orthophosphate and GDP. EC 6.3.4.4.
An enzyme that, in the course of purine ribonucleotide biosynthesis, catalyzes the conversion of 5'-phosphoribosyl-4-(N-succinocarboxamide)-5-aminoimidazole to 5'-phosphoribosyl-4-carboxamide-5-aminoimidazole and the conversion of adenylosuccinic acid to AMP. EC 4.3.2.2.
Inosine 5'-Monophosphate. A purine nucleotide which has hypoxanthine as the base and one phosphate group esterified to the sugar moiety.

Combinatorial interactions regulate cardiac expression of the murine adenylosuccinate synthetase 1 gene. (1/71)

The mammalian heart begins contracting at the linear tube stage during embryogenesis and continuously pumps, nonstop, throughout the entire lifetime of the animal. Therefore, the cardiac energy metabolizing pathways must be properly established and efficiently functioning. While the biochemistry of these pathways is well defined, limited information regarding the regulation of cardiac metabolic genes is available. Previously, we reported that 1.9 kilobase pairs of murine adenylosuccinate synthetase 1 gene (Adss1) 5'-flanking DNA directs high levels of reporter expression to the adult transgenic heart. In this report, we define the 1.9-kilobase pair fragment as a cardiac-specific enhancer that controls correct spatiotemporal expression of a reporter similar to the endogenous Adss1 gene. A 700-base pair fragment within this region activates a heterologous promoter specifically in adult transgenic hearts. Proteins present in a cardiac nuclear extract interact with potential transcription factor binding sites of this region and these cis-acting sites play important regulatory roles in the cardiac expression of this reporter. Finally, we report that several different cardiac transcription factors trans-activate the 1.9HSCAT construct through these sites and that combinations result in enhanced reporter expression. Adss1 appears to be one of the first target genes identified for the bHLH factors Hand1 and Hand2.  (+info)

Effectors of the stringent response target the active site of Escherichia coli adenylosuccinate synthetase. (2/71)

Guanosine 5'-diphosphate 3'-diphosphate (ppGpp), a pleiotropic effector of the stringent response, potently inhibits adenylosuccinate synthetase from Escherichia coli as an allosteric effector and/or as a competitive inhibitor with respect to GTP. Crystals of the synthetase grown in the presence of IMP, hadacidin, NO3-, and Mg2+, then soaked with ppGpp, reveal electron density at the GTP pocket which is consistent with guanosine 5'-diphosphate 2':3'-cyclic monophosphate. Unlike ligand complexes of the synthetase involving IMP and GDP, the coordination of Mg2+ in this complex is octahedral with the side chain of Asp13 in the inner sphere of the cation. The cyclic phosphoryl group interacts directly with the side chain of Lys49 and indirectly through bridging water molecules with the side chains of Asn295 and Arg305. The synthetase either directly facilitates the formation of the cyclic nucleotide or scavenges trace amounts of the cyclic nucleotide from solution. Regardless of its mode of generation, the cyclic nucleotide binds far more tightly to the active site than does ppGpp. Conceivably, synthetase activity in vivo during the stringent response may be sensitive to the relative concentrations of several effectors, which together exercise precise control over the de novo synthesis of AMP.  (+info)

Adenylosuccinate synthase from Saccharomyces cerevisiae: homologous overexpression, purification and characterization of the recombinant protein. (3/71)

Adenylosuccinate synthase (EC 6.3.4.4) catalyses the first committed step in the synthesis of adenosine. We have overexpressed the cloned gene of Saccharomyces cerevisiae (ADE12) in S. cerevisiae. The recombinant enzyme exhibits similar kinetic behaviour to that of the native enzyme purified from S. cerevisiae. This ter-reactant dimeric enzyme shows Michaelis-Menten kinetics only with IMP. l-Aspartate and GTP display a weak negative co-operativity (Hill coefficient 0. 8-0.9). This negative co-operativity has not yet been reported for adenylosuccinate synthases from other organisms. Another unusual feature of the enzyme from S. cerevisiae is its negligible inhibition by adenine nucleotides and its pronounced inhibition by Cl(-) ions.  (+info)

Electrical stimulation of neonatal cardiac myocytes activates the NFAT3 and GATA4 pathways and up-regulates the adenylosuccinate synthetase 1 gene. (4/71)

Electrically stimulated pacing of cultured cardiomyocytes serves as an experimentally convenient and physiologically relevant in vitro model of cardiac hypertrophy. Electrical pacing triggers a signaling cascade that results in the activation of the muscle-specific Adss1 gene and the repression of the nonmuscle Adss2 isoform. Activation of the Adss1 gene involves the calcineurin-mediated dephosphorylation of NFAT3, allowing its translocation to the nucleus, where it can directly participate in Adss1 gene activation. Mutational studies show that an NFAT binding site located in the Adss1 5'-flanking region is essential for this activation. Electrical pacing also results in the increased synthesis of GATA4, another critical cardiac transcription factor required for Adss1 gene expression. MEF2C also produces transactivation of the Adss1 gene reporter in control and paced cardiac myocytes. Using the Adss1 gene as a model, these studies are the first to demonstrate that electrical pacing activates the calcineurin/NFAT3 and GATA4 pathways as a means of regulating cardiac gene expression.  (+info)

Investigation of various genotype characteristics for inosine accumulation in Escherichia coli W3110. (5/71)

For the derivation of an inosine-overproducing strain from the wild type microorganism, it is known that the addition of an adenine requirement, removal of purine nucleoside hydrolyzing activity, removal of the feedback inhibition, and repression of key enzymes in the purine nucleotides biosynthetic pathway are essential. Thus, the disruption of purA (adenine requirement), deoD (removal of purine nucleosides phosphorylase activity), purR (derepression of the regulation of purine nucleotides biosynthetic pathway), and the insensitivity of the feedback inhibition of phosphoribosylpyrophosphate (PRPP) amidotransferase by adenosine 5'-monophosphate (AMP) and guanosine 5'-monophosphate (GMP) were done in the Escherichia coli strain W3110, and then the inosine productivity was estimated. In the case of using a plasmid harboring the PRPP amidotransferase gene (purF) that encoded a desensitized PRPP amidotransferase, purF disrupted mutants were used as the host strains. It was found that the innovation of the four genotypes brought about a small amount of inosine accumulation. Furthermore, an adenine auxotrophic mutant of E. coli showed inappropriate adenine use because its growth could not respond efficiently to the concentration of adenine added. As the presence of adenosine deaminase is well known in E. coli and it is thought to be involved in adenine use, a mutant disrupted adenosine deaminase gene (add) was constructed and tested. The mutant, which is deficient in purF, purA, deoD, purR, and add genes, and harboring the desensitized purF as a plasmid, accumulated about 1 g of inosine per liter. Although we investigated the effects of purR disruption and purF gene improvement, unexpectedly an increase in the inosine productivity could not be found with this mutant.  (+info)

Recombinant mouse muscle adenylosuccinate synthetase: overexpression, kinetics, and crystal structure. (6/71)

Vertebrates possess two isozymes of adenylosuccinate synthetase. The acidic isozyme is similar to the synthetase from bacteria and plants, being involved in the de novo biosynthesis of AMP, whereas the basic isozyme participates in the purine nucleotide cycle. Reported here is the first instance of overexpression and crystal structure determination of a basic isozyme of adenylosuccinate synthetase. The recombinant mouse muscle enzyme purified to homogeneity in milligram quantities exhibits a specific activity comparable with that of the rat muscle enzyme isolated from tissue and K(m) parameters for GTP, IMP, and l-aspartate (12, 45, and 140 microm, respectively) similar to those of the enzyme from Escherichia coli. The mouse muscle and E. coli enzymes have similar polypeptide folds, differing primarily in the conformation of loops, involved in substrate recognition and stabilization of the transition state. Residues 65-68 of the muscle isozyme adopt a conformation not observed in any previous synthetase structure. In its new conformation, segment 65-68 forms intramolecular hydrogen bonds with residues essential for the recognition of IMP and, in fact, sterically excludes IMP from the active site. Observed differences in ligand recognition among adenylosuccinate synthetases may be due in part to conformational variations in the IMP pocket of the ligand-free enzymes.  (+info)

IMP Alone Organizes the Active Site of Adenylosuccinate Synthetase from Escherichia coli. (7/71)

A complete set of substrate/substrate analogs of adenylosuccinate synthetase from Escherichia coli induces dimer formation and a transition from a disordered to an ordered active site. The most striking of the ligand-induced effects is the movement of loop 40-53 by up to 9 A. Crystal structures of the partially ligated synthetase, which either combine IMP and hadacidin or IMP, hadacidin, and Mg(2+)-pyrophosphate, have ordered active sites, comparable with the fully ligated enzyme. More significantly, a crystal structure of the synthetase with IMP alone exhibits a largely ordered active site, which includes the 9 A movement of loop 40-53 but does not include conformational adjustments to backbone carbonyl 40 (Mg(2+) interaction element) and loop 298-304 (L-aspartate binding element). Interactions involving the 5'-phosphoryl group of IMP evidently trigger the formation of salt links some 30 A away. The above provides a structural basis for ligand binding synergism, effects on k(cat) due to mutations far from the site of catalysis, and the complete loss of substrate efficacy due to minor alterations of the 5'-phosphoryl group of IMP.  (+info)

Determinants of L-aspartate and IMP recognition in Escherichia coli adenylosuccinate synthetase. (8/71)

Adenylosuccinate synthetase governs the first committed step in the de novo synthesis of AMP. Mutations of conserved residues in the synthetase from Escherichia coli reveal significant roles for Val(273) and Thr(300) in the recognition of l-aspartate, even though these residues do not or cannot hydrogen bond with the substrate. The mutation of Thr(300) to alanine increases the K(m) for l-aspartate by 30-fold. In contrast, its mutation to valine causes no more than a 4-fold increase in the K(m) for l-aspartate, while increasing k(cat) by 3-fold. Mutations of Val(273) to alanine, threonine, or asparagine increase the K(m) for l-aspartate from 15- to 40-fold, and concomitantly decrease the K(i) for dicarboxylate analogues of l-aspartate by up to 40-fold. The above perturbations are comparable with those resulting from the elimination of a hydrogen bond between the enzyme and substrate: alanine mutations of Thr(128) and Thr(129) increase the K(m) for IMP by up to 30-fold and the alanine mutation of Thr(301) abolishes catalysis supported by l-aspartate, but has no effect on catalysis supported by hydroxylamine. Structure-based mechanisms, by which the above residues influence substrate recognition, are presented.  (+info)

This gene encodes a member of the adenylosuccinate synthase family of proteins. The encoded muscle-specific enzyme plays a role in the purine nucleotide cycle by catalyzing the first step in the conversion of inosine monophosphate (IMP) to adenosine monophosphate (AMP). Mutations in this gene may cause adolescent onset distal myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016 ...
TY - JOUR. T1 - Role of purine synthesis on renal function. T2 - effect of adenylosuccinate synthetase inhibition.. AU - Lien, Y. H.. AU - Lai, L. W.. AU - Cheung, C.. AU - Patterson, D.. AU - Chan, L.. PY - 1991. Y1 - 1991. UR - http://www.scopus.com/inward/record.url?scp=0026298144&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0026298144&partnerID=8YFLogxK. M3 - Article. C2 - 1807901. AN - SCOPUS:0026298144. VL - 95. SP - 112. EP - 119. JO - Contributions to Nephrology. JF - Contributions to Nephrology. SN - 0302-5144. ER - ...
Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.
Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.
Adenylosuccinate synthetase; Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP; Belongs to the adenylosuccinate synthetase family (333 aa ...
1CG4: Mechanistic implications from crystalline complexes of wild-type and mutant adenylosuccinate synthetases from Escherichia coli.
1CG3: Mechanistic implications from crystalline complexes of wild-type and mutant adenylosuccinate synthetases from Escherichia coli.
ID D1YV79_METPS Unreviewed; 337 AA. AC D1YV79; DT 09-FEB-2010, integrated into UniProtKB/TrEMBL. DT 09-FEB-2010, sequence version 1. DT 11-DEC-2019, entry version 67. DE RecName: Full=Adenylosuccinate synthetase {ECO:0000256,HAMAP-Rule:MF_00011, ECO:0000256,RuleBase:RU000520}; DE Short=AMPSase {ECO:0000256,HAMAP-Rule:MF_00011}; DE Short=AdSS {ECO:0000256,HAMAP-Rule:MF_00011}; DE EC=6.3.4.4 {ECO:0000256,HAMAP-Rule:MF_00011, ECO:0000256,RuleBase:RU000520}; DE AltName: Full=IMP--aspartate ligase {ECO:0000256,HAMAP-Rule:MF_00011}; GN Name=purA {ECO:0000256,HAMAP-Rule:MF_00011, GN ECO:0000313,EMBL:BAI60351.1}; GN OrderedLocusNames=MCP_0279 {ECO:0000313,EMBL:BAI60351.1}; OS Methanocella paludicola (strain DSM 17711 / JCM 13418 / NBRC 101707 / OS SANAE). OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia; OC Methanocellales; Methanocellaceae; Methanocella. OX NCBI_TaxID=304371 {ECO:0000313,EMBL:BAI60351.1, ECO:0000313,Proteomes:UP000001882}; RN [1] {ECO:0000313,Proteomes:UP000001882} RP ...
K. Eaazhisai, R. Jayalakshmi, P. Gayathri, R. P. Anand, K. Sumathy, H. Balaram and M. R. N. Murthy, Crystal structure of fully-ligated adenylosuccinate synthetase from Plasmodium falciparum, Journal of Molecular Biology 335, 1251 - 1264 (2004 ...
K. Eaazhisai, R. Jayalakshmi, P. Gayathri, R. P. Anand, K. Sumathy, H. Balaram and M. R. N. Murthy, Crystal structure of fully-ligated adenylosuccinate synthetase from Plasmodium falciparum, Journal of Molecular Biology 335, 1251 - 1264 (2004 ...
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Adenylosuccinate lyase is an enzyme of fumarase superfamily that participates in the purine biosynthetic pathway, catalysing the nonhydrolytic cleavage of succinyl groups from SAICA ribotide and adenylosuccinate. Enzyme defects are associated with a
SWISS-MODEL Repository entry for A8Q0E3 (PURA_MALGO), Adenylosuccinate synthetase. Malassezia globosa (strain ATCC MYA-4612 / CBS 7966)(Dandruff-associated fungus)
Spontaneous mutants arise among nondividing populations of Escherichia coli in apparent response to selective conditions. In this report we investigate several hypotheses to account for the role of selection in the production of these directed or adaptive mutations. We found that the Lac+ phenotypes of some mutants that arise late after lactose selection are due to suppressor mutations that are unlinked to the mutant lacZ allele; thus the production of these Lac+ mutants does not require an information flow from successful proteins back to the DNA that encodes them. Transcriptional induction of the lac operon, even in the presence of another, utilizable carbon source, did not stimulate the occurrence of Lac+ mutants in the absence of lactose, indicating that the role of the selective agent is not merely to induce transcription. The absence of two DNA repair pathways-methyl-directed mismatch repair and alkylation repair-also did not result in an accumulation of Lac+ mutants in the absence of ...
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Definition of adenylosuccinate lyase. Provided by Stedmans medical dictionary and Drugs.com. Includes medical terms and definitions.
Adenylosuccinate lyase (ASL) catalyzes 2 reactions in the de novo synthesis of purine nucleotides. In humans, point mutations of this enzyme are associated with mental retardation, autism, and muscle wasting. The sequence of B. subtilis ASL is 27% identical and 17% similar to the human enzyme. We have cloned, expressed, and purified B. subtilis ASL; the crystal structure of a 73% homologous bacterial enzyme is known (PDB #lc3u). We separately mutated 2 amino acids in the B. subtilis ASL (M10L & N276C) equivalent to the mutations identified in human ASL deficiency (M26L & R303C). The purified M10L and N276C possess specific activities of 0.86 and 0.38 units/mg, respectively, as compared to 1.56 units/mg for wild type (WT) enzyme. Both enzymes are similar to WT in conformation and oligomeric state as indicated by circular dichroism and light scattering. The Km for adenylosuccinate (SAMP) is not appreciably different between M1OL (5.6 uM) and WT (4.9 uM); and the pH dependence of Vmax is also ...
Berry, A and Vamplew, P (2004) PoD Can Mutate: A Simple Dynamic Directed Mutation Approach for Genetic Algorithms. In: AISAT2004: International Conference on Artificial Intelligence in Science and Technology, 21-25 November 2004, Hobart, Tasmania, Australia. ...
Adenylosuccinate Lyase; Catalyzes Two Steps In The de Novo Purine Nucleotide Biosynthetic Pathway; Expression Is Repressed By Adenine And Activated By Bas1p And Pho2p; Mutations In Human Ortholog ADSL Cause Adenylosuccinase Deficiency
Adenylosuccinate Lyase; Catalyzes Two Steps In The de Novo Purine Nucleotide Biosynthetic Pathway; Expression Is Repressed By Adenine And Activated By Bas1p And Pho2p; Mutations In Human Ortholog ADSL Cause Adenylosuccinase Deficiency
Banerjee, Sanchari and Agrawal, Monika J and Mishra, Diptimayee and Sharan, Siddharth and Balaram, Hemalatha and Savithri, Handanhal S and Murthy, Mathur RN (2014) Structural and kinetic studies on adenylosuccinate lyase from Mycobacterium smegmatis and Mycobacterium tuberculosis provide new insights on the catalytic residues of the enzyme. In: FEBS JOURNAL, 281 (6). pp. 1642-1658. Chittori, Sagar and Simanshu, Dhirendra Kumar and Banerjee, Sanchari and Murthy, Ambika Mosale Venkatesh and Mathivanan, Subashini and Savithri, Handanahal Subbarao and Murthy, Mathur Ramabhadrashastry Narasimha (2013) Mechanistic features of Salmonella typhimurium propionate kinase (TdcD): Insights from kinetic and crystallographic studies. In: BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1834 (10). pp. 2036-2044. ...
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A good complement to a junk DNA FAQ would be a mechanisms of mutation FAQ, which I dont think exists anywhere. People seem to treat mutation as some kind of magically process and therefore get mislead into all kinds of directed mutation, etc. stuff ...
A good complement to a junk DNA FAQ would be a mechanisms of mutation FAQ, which I dont think exists anywhere. People seem to treat mutation as some kind of magically process and therefore get mislead into all kinds of directed mutation, etc. stuff ...
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Terms of use. Disclaimer. Privacy Statement.. The information contained on this site is for general informational purposes only. It is not a substitute for expert medical advice, and does not take your medical history or specific circumstances into consideration. Always seek your doctors advice if you have a medical or health-related condition. While every effort has been made to ensure that the above information was correct at the time of writing; medical knowledge is constantly being reviewed and updated, and the above information may therefore be superseded by additional knowledge.. ...
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TY - JOUR. T1 - Genetic demonstration of bidirectionality in the high affinity purine base transporter of mutant mouse S49 cells.. AU - Beck, J. T.. AU - Ullman, B.. N1 - Copyright: Medline is the source for the citation and abstract of this record.. PY - 1987/2/15. Y1 - 1987/2/15. N2 - A mutant cell line was selected from wild type S49 lymphoblasts that expressed a novel high affinity purine base transport system not found in parental cells or any other mammalian cell line (Aronow, B., Toll, D., Patrick, J., Hollingsworth, P., McCartan, K., and Ullman, B. (1986) Mol. Cell. Biol. 6, 2957-2962). In order to determine whether this nucleobase transport system was bidirectional, mutant cell lines possessing this high affinity base transport capability were derived from a nucleoside transport-deficient derivative of an adenylosuccinate synthetase-deficient S49 cell line. The resulting progeny excreted significantly greater amounts of purine into the cell culture medium than parental cells. This ...
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Terms of use. Disclaimer. Privacy Statement.. The information contained on this site is for general informational purposes only. It is not a substitute for expert medical advice, and does not take your medical history or specific circumstances into consideration. Always seek your doctors advice if you have a medical or health-related condition. While every effort has been made to ensure that the above information was correct at the time of writing; medical knowledge is constantly being reviewed and updated, and the above information may therefore be superseded by additional knowledge.. ...
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Humans express two adenylosuccinate synthase isozymes: Adenylosuccinate+synthase at the US National Library of Medicine Medical ... In molecular biology, adenylosuccinate synthase (or adenylosuccinate synthetase) (EC 6.3.4.4) is an enzyme that plays an ... Adenylosuccinate synthetase has been characterised from various sources ranging from Escherichia coli (gene purA) to vertebrate ... Structures of adenylosuccinate synthetase from Triticum aestivum and Arabidopsis thaliana when compared with the known ...
Adenylosuccinate synthase like 1 is a protein that in humans is encoded by the ADSS1 gene. This gene encodes a member of the ... "Entrez Gene: Adenylosuccinate synthase like 1". Retrieved 2018-04-09. "Cure ADSSL1". Retrieved 2022-01-30. Sun H, Li N, Wang X ... adenylosuccinate synthase family of proteins. The encoded muscle-specific enzyme plays a role in the purine nucleotide cycle by ... "Molecular cloning and characterization of a novel muscle adenylosuccinate synthetase, AdSSL1, from human bone marrow stromal ...
First, GTP hydrolysis fuels the addition of aspartate to IMP by adenylosuccinate synthase, substituting the carbonyl oxygen for ... This step is catalyzed by adenylosuccinate lyase. Inosine monophosphate is converted to guanosine monophosphate by the ... a nitrogen and forming the intermediate adenylosuccinate. Fumarate is then cleaved off forming adenosine monophosphate. ...
... this is subsequently acted upon by adenylosuccinate synthase and adenylosuccinate lyase, in a two step process, to convert it ... kinetic and catalytic mechanism of adenylosuccinate lyase from Plasmodium falciparum". Biochimica et Biophysica Acta (BBA) - ...
The enzyme adenylosuccinate synthase carries out the reaction by the addition of aspartate to IMP and requires the input of ... Adenylosuccinate lyase deficiency Purine nucleotide cycle Figures 20.4 and 20.7 in Textbook of Biochemistry, with clinical ... Adenylosuccinate is an intermediate in the interconversion of purine nucleotides inosine monophosphate (IMP) and adenosine ...
... adenylosuccinate synthase, phosphopyruvate hydratase, RNA-associated protein, threonine synthase, aspartate aminotransferase, ... precorrin-8x methylmutase, protoporphyrin IX magnesium chelatase and geranylgeranylglyceryl phosphate synthase-like protein. ...
GMP back into IMP adenylosuccinate synthase converts IMP to adenylosuccinate adenylosuccinate lyase converts adenylosuccinate ... AICAR + fTHF → FAICAR + THF The last step is catalyzed by Inosine monophosphate synthase. FAICAR → IMP + H2O In eukaryotes the ... CAIR + L-Aspartate + ATP → SAICAR + ADP + Pi The eight is catalyzed by adenylosuccinate lyase. SAICAR → AICAR + Fumarate The ... IMP dehydrogenase (IMPDH) converts IMP into XMP GMP synthase converts XMP into GMP GMP reductase converts ...
... adenylosuccinate synthase, adenine phosphoribosyltransferases, and guanine phosphoribosyltransferases. The human subjects ...
... a type of fibre optic cable used by electrical power transmission companies Adenylosuccinate synthase, an enzyme This ...
IMP may also be generated into AMP by adenylosuccinate synthetase and then adenylosuccinate lyase. GMP synthase is also ... GMP synthase forms a tetramer in an open box shape, which is a dimer of dimers. The R interfaces are held together with a ... GMP synthase is the second step in the generation of GMP from IMP; the first step occurs when IMP dehydrogenase generates XMP, ... PDBe-KB provides an overview of all the structure information available in the PDB for Human GMP synthase [glutamine- ...
... adenylosuccinate synthase MeSH D08.811.464.259.200 - amide synthases MeSH D08.811.464.259.200.200 - aspartate-ammonia ligase ... riboflavin synthase MeSH D08.811.913.225.825 - spermidine synthase MeSH D08.811.913.225.912 - spermine synthase MeSH D08.811. ... nitric oxide synthase type i MeSH D08.811.682.664.500.772.500 - nitric oxide synthase type ii MeSH D08.811.682.664.500.772.750 ... glycogen synthase kinases MeSH D08.811.913.696.620.682.700.429.500 - glycogen synthase kinase 3 MeSH D08.811.913.696.620.682. ...
An example of such a conformational disease is ALAD porphyria, which results from a mutation of porphobilinogen synthase that ... Palenchar, Jennifer Brosius; Colman, Roberta F. (2003). "Characterization of a Mutant Bacillus subtilis Adenylosuccinate Lyase ... The one protein that is established to function as a morpheein is porphobilinogen synthase, though there are suggestions ... An inhibitor of porphobilinogen synthase with this mechanism of action has been documented. The morpheein model of allosteric ...
... adenylosuccinate synthase EC 6.3.4.5: argininosuccinate synthase EC 6.3.4.6: urea carboxylase EC 6.3.4.7: ribose-5-phosphate- ... c-diamide synthase EC 6.3.5.12: Ni-sirohydrochlorin a,c-diamide synthase * EC 6.3.5.13: lipid II isoglutaminyl synthase ( ... thioglycine synthase * EC 6.2.2.2: oxazoline synthase * EC 6.2.2.3: thiazoline synthase * * No Wikipedia article EC 6.3.1.1: ... 2-hydroxypropanoate synthase * EC 6.3.2.56: staphyloferrin B synthase * EC 6.3.2.57: staphyloferrin A synthase * EC 6.3.2.58: D ...
11-diene synthase EC 4.2.3.25: S-linalool synthase EC 4.2.3.26: R-linalool synthase EC 4.2.3.27: isoprene synthase EC 4.2.3.28 ... adenylosuccinate lyase EC 4.3.2.3: ureidoglycolate lyase EC 4.3.2.4: purine imidazole-ring cyclase EC 4.3.2.5: ... α-santalene synthase EC 4.2.3.83: β-santalene synthase EC 4.2.3.84: 10-epi-γ-eudesmol synthase EC 4.2.3.85: α-eudesmol synthase ... cubebol synthase EC 4.2.3.92: (+)-γ-cadinene synthase EC 4.2.3.93: δ-guaiene synthase EC 4.2.3.94: γ-curcumene synthase EC 4.2. ...
It was recently demonstrated that a homologue of adenylosuccinate synthase (PurZ) and a dATP triphosphohydrolase (DatZ) are two ... It was recently demonstrated that a homologue of adenylosuccinate synthetase (PurZ) and a dATP triphosphohydrolase (DatZ) are ...
adenylosuccinate synthase. 9. SEQF2091,AEHQ01000092.1. EFO69943.1 jb [NA] [AA] 222/73. 12780-13001. hypothetical protein. ... hydroxymethylglutaryl-CoA synthase. 87. SEQF2091,AEHQ01000087.1. EFO70021.1 jb [NA] [AA] 1131/376. 16756-15626. CamS sex ...
We previously discovered that monosulfuron ester sodium (MES), an acetolactate synthase (ALS) inhibitor of the herbicide ... adenylosuccinate synthase (At3g57610), transketolase family protein (At2g34590), and pyruvate kinase family protein (At3g22960 ... Unraveling the role of fermentation in the mode of action of acetolactate synthase inhibitors by metabolic profiling. J Plant ... Crystal structures of two novel sulfonylurea herbicides in complex with Arabidopsis thaliana acetohydroxyacid synthase. FEBS J ...
adenylosuccinate synthase 2 [Sou.... NM_001127235. 65056. GPBP1. GC-rich promoter binding protein.... ...
Modification pertaining to: Adenylosuccinate synthase doasage amounts sufficient to supply lifetime. in U.S. Civil War. ...
Adenylosuccinate synthase Increases the Usefulness regarding APR-246 via Induction associated with G. in U.S. Civil War. ...
Adenylosuccinate synthase - Concept préféré Concept UI. M0000415. Terme préféré. Adenylosuccinate synthase Synonymes. IMP- ... During purine ribonucleotide biosynthesis, this enzyme catalyzes the synthesis of adenylosuccinate from GTP; IMP; and aspartate ... Adenylosuccinate synthase Descripteur en anglais: Adenylosuccinate Synthase Descripteur en espagnol: Adenilosuccinato Sintasa ...
Adenylosuccinate synthetase ADSS AT3G57610.1 Solyc02g085520.2.1 SL2.40ch02 NA adenylosuccinate synthase [104] ...
adenylosuccinate synthase activity. IEP. Enrichment. MF. GO:0004190. aspartic-type endopeptidase activity. IEP. Enrichment. ... proton-transporting ATP synthase activity, rotational mechanism. IEP. Enrichment. MF. GO:0050136. NADH dehydrogenase (quinone) ... indole-3-glycerol-phosphate synthase activity. IEP. Enrichment. MF. GO:0004476. mannose-6-phosphate isomerase activity. IEP. ...
Adenylosuccinate synthase Deficiency. *. Alexander disease. *. Alpers-Huttenlocher syndrome. *. Amyloidoses. *. Argyrophilic ...
adenylosuccinate synthase activity. IEP. Enrichment. MF. GO:0004177. aminopeptidase activity. IEP. Enrichment. ...
adenylosuccinate synthetase like 1. 12 11565 Gene Ontology adenylosuccinate synthase activity , adenylosuccinate synthase ...
... adenylosuccinate synthase 1, and spinocerebellar ataxia.. The news is yet another bump in the road for the close-knit DMD ...
Specifically, we identified the adenylosuccinate synthase mutated strain (ΔpurA) infection exhibited a robust activation of NF- ... and the mRNA level of putative CMP-Neu5Ac synthase (css) is upregulated compared to that in the non-pathogenic strain. However ...
Adenylosuccinate lyase (ADSL) deficiency is an autosomal recessive defect of purine metabolism, affecting purinosome assembly ... Uridine monophosphate synthase deficiency (UMPS, OMIM 613891). • Beta-ureidopropionase deficiency (UPB1, OMIM 613161) ... Type I adenylosuccinate lyase deficiency (severe). Most patients reported so far have adenylosuccinate lyase deficiency type I ... Adenylosuccinate lyase deficiency. Adenylosuccinate lyase deficiency, the first enzyme deficiency reported in the DNPS pathway ...
Adenylosuccinate Synthase [D08.811.464.259.100] Adenylosuccinate Synthase * Amide Synthases [D08.811.464.259.200] ... Peptide Synthases - Preferred Concept UI. M0016235. Scope note. Ligases that catalyze the joining of adjacent AMINO ACIDS by ... Peptide Synthases Entry term(s). Acid Amino Acid Ligases Acid-Amino-Acid Ligases Ligases, Acid-Amino-Acid Peptide Synthetases ...
XTP + IMP + L-aspartate <=> XDP + phosphate + adenylosuccinate 6.3.4.4 adenylosuccinate synthase - BRENDA: BS711 ...
AASS: aminoadipate-semialdehyde synthase. *ABAT: 4-aminobutyrate aminotransferase. *ABCA1: ATP binding cassette subfamily A ... ADSL: adenylosuccinate lyase. *AFF2: ALF transcription elongation factor 2. *AFF4: ALF transcription elongation factor 4 ...
As proteínas Adenylosuccinate lyase (ADSL) e Alpha-enolase (ENO1), foram observadas apenas em amostras de animais castrados. A ... Fatty acid synthase (FASN), Peroxisome proliferator activated receptor gamma (PPARG) and Stearoyl-CoA desaturase (SCD) in the ... The proteins Adenylosuccinate lyase (ADSL) e Alpha-enolase (ENO1) were observed only in samples from steers. Castration, ... Fatty acid synthase (FASN), Peroxisome proliferator activated receptor gamma (PPARG) e Stearoyl-CoA desaturase (SCD) no músculo ...
EC 6.3.4.3 formate-tetrahydrofolate ligase EC 6.3.4.4 adenylosuccinate synthase EC 6.3.4.5 argininosuccinate synthase EC 6.3. ... N6-hydroxylysine synthase EC 6.3.2.39 aerobactine synthase EC 6.3.2.40 cyclopeptine synthase EC 6.3.2.41 N- ... EC 6.3.5.1 NAD+ synthase (glutamine-hydrolyse) EC 6.3.5.2 GMP synthase (glutamine-hydrolyse) EC 6.3.5.3 ... acetylaspartylglutamate synthase EC 6.3.2.42 N-acetylaspartylglutamylglutamate synthase EC 6.3.3 Cyclo-Ligases (des enzymes qui ...
Adenylosuccinate Lyase Deficiency (PathBank: SMP0000167). *Gout or Kelley-Seegmiller Syndrome (PathBank: SMP0000365) ... Cystathionine beta-Synthase Deficiency (PathBank: SMP0000177). *Hypermethioninemia (PathBank: SMP0000341). *S- ...
pseudouridine synthase. 93. SEQF2753,CP012074.1. AKU68404.1 jb [NA] [AA] 1347/448. 135924-134578. adenylosuccinate lyase. ... lipid-A-disaccharide synthase. 139. SEQF2753,CP012074.1. AKU68450.1 jb [NA] [AA] 774/257. 215521-214748. stationary phase ... molybdopterin-synthase small subunit. 85. SEQF2753,CP012074.1. AKU68396.1 jb [NA] [AA] 603/200. 125211-125813. hypothetical ... 1-deoxy-D-xylulose-5-phosphate synthase. 102. SEQF2753,CP012074.1. AKU68413.1 jb [NA] [AA] 1341/446. 152324-153664. potassium ...
ATP synthase delta (OSCP) subunit [Interproscan].","protein_coding" "CPD24366","atpF","Staphylococcus aureus","ATP synthase B ... ","adenylosuccinate lyase [Ensembl]. Adenylosuccinate lyase C-terminus [Interproscan].","protein_coding" "CPD13571","rplW"," ... ","CTP synthase [Ensembl]. CTP synthase N-terminus, Glutamine amidotransferase class-I [Interproscan].","protein_coding" " ... ","Tryptophan synthase alpha chain [Ensembl]. Tryptophan synthase alpha chain [Interproscan].","protein_coding" "AKP15633","rnc ...
... "adenylosuccinate synthetase,adenylosuccinate synthase," YLR322W -0.015784 INESSENTIAL "biological_process unknown, molecular_ ... "CTP synthase, highly homologus to URA8 CTP synthase,CTP synthase, " YMR191W -0.088604 INESSENTIAL "biological_process unknown, ... "para-aminobenzoate synthase, PABA synthase, ribosomal large subunit nucleus export, para-aminobenzoic acid (PABA) synthase, ... "alpha-isopropylmalate synthase (2-Isopropylmalate Synthase),2-isopropylmalate synthase, " YPR075C 5.027926 INESSENTIAL OPY2 ...
Name: ceramide synthase 5. Synonyms: 2310081H14Rik, Trh4, CerS5, Lass5. Type: Gene. Species: Mus musculus (mouse) ... Name: adenylosuccinate synthetase like 1. Synonyms: Adss. Type: Gene. Species: Mus musculus (mouse) ...
Adenylosuccinate synthase, GTP-binding site 30 Domain IPR002192:Pyruvate phosphate dikinase, PEP/pyruvate-binding 30 Domain ... Adenylosuccinate synthase, active site 33 Family IPR016215:Nitrilotriacetate monooxygenase component A/pristinamycin IIA ... NAD/GMP synthase 76 Domain IPR001667:DDH domain 76 Domain IPR013747:3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III, C- ... Thiamine phosphate synthase/TenI 36 Active_site IPR019810:Citrate synthase active site 36 Active_site IPR033524:Leu/Phe/Val ...
Asparagine synthase (glutamine-hydrolysing); Potential phenazine-modifying enzyme; asn_synth_AEB: asparagine synthase ... Adenylosuccinate synthetase; Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the ... Asparagine synthase (glutamine-hydrolysing); Potential phenazine-modifying enzyme; asn_synth_AEB: asparagine synthase ... Asparagine synthase (glutamine-hydrolysing); Potential phenazine-modifying enzyme; asn_synth_AEB: asparagine synthase ...
  • It was recently demonstrated that a homologue of adenylosuccinate synthetase (PurZ) and a dATP triphosphohydrolase (DatZ) are two important pieces. (researchgate.net)
  • Adenylosuccinate lyase deficiency is a rare, autosomal recessive defect of purine metabolism affecting purinosome assembly and reducing metabolite fluxes through both de novo purine synthesis (DNPS) and purine nucleotide recycling pathways. (medlink.com)
  • Clinically, adenylosuccinate lyase deficiency is generally categorized into three phenotypes: a fatal neonatal form, a severe form (type I), and a milder form (type II). (medlink.com)
  • Neurologic symptoms are the most common and prominent clinical problems associated with adenylosuccinate lyase deficiency. (medlink.com)
  • Diagnosis of adenylosuccinate lyase deficiency may be delayed or missed because patients can present with nonspecific features, such as developmental delay, autism spectrum disorder, or epilepsy. (medlink.com)
  • Adenylosuccinate lyase deficiency can be diagnosed by detection of elevated metabolites along the DNPS pathway (succinylpurines) in body fluids. (medlink.com)
  • No specific FDA-approved treatment is available for adenylosuccinate lyase deficiency. (medlink.com)
  • The clinical presentation of adenylosuccinate lyase deficiency varies greatly with respect to age of onset, clinical manifestations, and rate of disease progression. (medlink.com)
  • Patients with adenylosuccinate lyase deficiency can present with nonspecific symptoms, such as developmental delay, autism spectrum disorder, or epilepsy, including infantile spasms. (medlink.com)
  • Selective screening for adenylosuccinate lyase deficiency should be performed in infants who have neurologic disease without clear etiology, especially if supportive MRI findings are also present (eg, delayed or lack of myelination, abnormal white matter signal, and atrophy of the cerebrum or cerebellum). (medlink.com)
  • Detection of succinylpurines in body fluids by high-performance liquid chromatography or liquid chromatography-tandem mass spectrometry is the preferred biochemical test for adenylosuccinate lyase deficiency. (medlink.com)
  • Corinna's research uses C. elegans with a reduced adenylosuccinate lyase activity to study neurobehavioral manifestations associated with the human disease adenylosuccinate lyase deficiency (ASLD). (psu.edu)
  • The purinosome is a multienzyme complex of DNPS enzymes (including adenylosuccinate lyase) that cells transiently assemble in their cytosol to address both depletion of and increased demand for purines. (medlink.com)
  • We previously discovered that monosulfuron ester sodium (MES), an acetolactate synthase (ALS) inhibitor of the herbicide sulfonylurea family, can induce rapeseed ( Brassica napus L.) male sterility at approximately 1% concentration required for its herbicidal activity. (biomedcentral.com)
  • Attention cependant à ne pas confondre les enzymes synthétases avec les enzymes synthases (qui sont toutes deux des enzymes du groupe des ligases). (wikipedia.org)
  • En biochimie , une ligase est une enzyme qui catalyse la jonction de deux molécules (en anglais ligation ) par de nouvelles liaisons covalentes avec hydrolyse concomitante de l' ATP ou d'autres molécules similaires. (wikipedia.org)
  • As proteínas Adenylosuccinate lyase (ADSL) e Alpha-enolase (ENO1), foram observadas apenas em amostras de animais castrados. (unesp.br)
  • Observou-se maiores expressões dos genes lipogênicos: Acetyl-CoA carboxylase alpha (ACACA), Fatty acid binding protein 4, adipocyte (FABP4), Fatty acid synthase (FASN), Peroxisome proliferator activated receptor gamma (PPARG) e Stearoyl-CoA desaturase (SCD) no músculo longissimus thoracis de animais castrados, quando comparada aos inteiros. (unesp.br)
  • Belongs to the argininosuccinate synthase family. (string-db.org)
  • We examined whether ITP can be used by adenosine triphosphatase (ATPase) in human erythrocytes or recombinant human adenylosuccinate synthase (ADSS). (nih.gov)
  • Three enzymes involved in catalyzing the formation of NTP analogs were noted to be highly expressed in surviving cells: adenosine kinase, adenylosuccinate synthase, and NUDT2. (azolifesciences.com)
  • When the product is more important, synthase may be used in the name, e.g. phosphosulfolactate synthase (EC 4.4.1.19, Michael addition of sulfite to phosphoenolpyruvate). (ipfs.io)
  • Encodes a phosphatidylglycerolphosphate synthase 2C which is dual-targeted into chloroplasts and mitochondria. (or.jp)
  • This gene encodes a member of the adenylosuccinate synthase family of proteins. (nih.gov)