Species of the genus MASTADENOVIRUS, causing a wide range of diseases in humans. Infections are mostly asymptomatic, but can be associated with diseases of the respiratory, ocular, and gastrointestinal systems. Serotypes (named with Arabic numbers) have been grouped into species designated Human adenovirus A-F.
A family of non-enveloped viruses infecting mammals (MASTADENOVIRUS) and birds (AVIADENOVIRUS) or both (ATADENOVIRUS). Infections may be asymptomatic or result in a variety of diseases.
Respiratory and conjunctival infections caused by 33 identified serotypes of human adenoviruses.
Proteins transcribed from the E1A genome region of ADENOVIRUSES which are involved in positive regulation of transcription of the early genes of host infection.
Virus diseases caused by the ADENOVIRIDAE.
Proteins encoded by adenoviruses that are synthesized prior to, and in the absence of, viral DNA replication. The proteins are involved in both positive and negative regulation of expression in viral and cellular genes, and also affect the stability of viral mRNA. Some are also involved in oncogenic transformation.
Proteins transcribed from the E1B region of ADENOVIRUSES which are involved in regulation of the levels of early and late viral gene expression.
Proteins transcribed from the E3 region of ADENOVIRUSES but not essential for viral replication. The E3 19K protein mediates adenovirus persistence by reducing the expression of class I major histocompatibility complex antigens on the surface of infected cells.
Proteins transcribed from the E4 region of ADENOVIRUSES. The E4 19K protein transactivates transcription of the adenovirus E2F protein and complexes with it.
The very first viral gene products synthesized after cells are infected with adenovirus. The E1 region of the genome has been divided into two major transcriptional units, E1A and E1B, each expressing proteins of the same name (ADENOVIRUS E1A PROTEINS and ADENOVIRUS E1B PROTEINS).
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
Species of the genus MASTADENOVIRUS that causes fever, edema, vomiting, and diarrhea in dogs and encephalitis in foxes. Epizootics have also been caused in bears, wolves, coyotes, and skunks. The official species name is Canine adenovirus and it contains two serotypes.
Proteins transcribed from the E2 region of ADENOVIRUSES. Several of these are required for viral DNA replication.
A genus of ADENOVIRIDAE that infects MAMMALS including humans and causes a wide range of diseases. The type species is Human adenovirus C (see ADENOVIRUSES, HUMAN).
Species of the genus MASTADENOVIRUS, causing neurological disease in pigs.
A genus of ADENOVIRIDAE that infects birds. The type species is FOWL ADENOVIRUS A.
Techniques and strategies which include the use of coding sequences and other conventional or radical means to transform or modify cells for the purpose of treating or reversing disease conditions.
The type species of the genus AVIADENOVIRUS, family ADENOVIRIDAE, an oncogenic virus of birds. This is also called CELO virus for chick embryo lethal orphan virus.
Deoxyribonucleic acid that makes up the genetic material of viruses.
The introduction of functional (usually cloned) GENES into cells. A variety of techniques and naturally occurring processes are used for the gene transfer such as cell hybridization, LIPOSOMES or microcell-mediated gene transfer, ELECTROPORATION, chromosome-mediated gene transfer, TRANSFECTION, and GENETIC TRANSDUCTION. Gene transfer may result in genetically transformed cells and individual organisms.
Proteins that form the CAPSID of VIRUSES.
The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
Established cell cultures that have the potential to propagate indefinitely.
The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.
Specific molecular components of the cell capable of recognizing and interacting with a virus, and which, after binding it, are capable of generating some signal that initiates the chain of events leading to the biological response.
Use of attenuated VIRUSES as ANTINEOPLASTIC AGENTS to selectively kill CANCER cells.
Tumor-selective, replication competent VIRUSES that have antineoplastic effects. This is achieved by producing cytotoxicity-enhancing proteins and/or eliciting an antitumor immune response. They are genetically engineered so that they can replicate in CANCER cells but not in normal cells, and are used in ONCOLYTIC VIROTHERAPY.
Simultaneous inflammation of the cornea and conjunctiva.
The transfer of bacterial DNA by phages from an infected bacterium to another bacterium. This also refers to the transfer of genes into eukaryotic cells by viruses. This naturally occurring process is routinely employed as a GENE TRANSFER TECHNIQUE.
Proteins found in any species of virus.
The functional hereditary units of VIRUSES.
Products of viral oncogenes, most commonly retroviral oncogenes. They usually have transforming and often protein kinase activities.
Inflammation, often mild, of the conjunctiva caused by a variety of viral agents. Conjunctival involvement may be part of a systemic infection.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Vaccines used to prevent infection by any virus from the family ADENOVIRIDAE.
The outer protein protective shell of a virus, which protects the viral nucleic acid.
An inheritable change in cells manifested by changes in cell division and growth and alterations in cell surface properties. It is induced by infection with a transforming virus.
Genes that are introduced into an organism using GENE TRANSFER TECHNIQUES.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Visible morphologic changes in cells infected with viruses. It includes shutdown of cellular RNA and protein synthesis, cell fusion, release of lysosomal enzymes, changes in cell membrane permeability, diffuse changes in intracellular structures, presence of viral inclusion bodies, and chromosomal aberrations. It excludes malignant transformation, which is CELL TRANSFORMATION, VIRAL. Viral cytopathogenic effects provide a valuable method for identifying and classifying the infecting viruses.
Viruses which enable defective viruses to replicate or to form a protein coat by complementing the missing gene function of the defective (satellite) virus. Helper and satellite may be of the same or different genus.
Viruses which lack a complete genome so that they cannot completely replicate or cannot form a protein coat. Some are host-dependent defectives, meaning they can replicate only in cell systems which provide the particular genetic function which they lack. Others, called SATELLITE VIRUSES, are able to replicate only when their genetic defect is complemented by a helper virus.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.
Ribonucleic acid that makes up the genetic material of viruses.
Any of the processes by which cytoplasmic factors influence the differential control of gene action in viruses.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
A genus of the family PICORNAVIRIDAE whose members preferentially inhabit the intestinal tract of a variety of hosts. The genus contains many species. Newly described members of human enteroviruses are assigned continuous numbers with the species designated "human enterovirus".
The process by which a DNA molecule is duplicated.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
A genus of ADENOVIRIDAE that comprises viruses of several species of MAMMALS and BIRDS. The type species is Ovine adenovirus D.
A species of POLYOMAVIRUS originally isolated from Rhesus monkey kidney tissue. It produces malignancy in human and newborn hamster kidney cell cultures.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
Mutant mice homozygous for the recessive gene "nude" which fail to develop a thymus. They are useful in tumor studies and studies on immune responses.
Process of growing viruses in live animals, plants, or cultured cells.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
The complete genetic complement contained in a DNA or RNA molecule in a virus.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
A cell line derived from cultured tumor cells.
Invasion of the host RESPIRATORY SYSTEM by microorganisms, usually leading to pathological processes or diseases.
Production of new arrangements of DNA by various mechanisms such as assortment and segregation, CROSSING OVER; GENE CONVERSION; GENETIC TRANSFORMATION; GENETIC CONJUGATION; GENETIC TRANSDUCTION; or mixed infection of viruses.
Immunoglobulins produced in response to VIRAL ANTIGENS.
Minute infectious agents whose genomes are composed of DNA or RNA, but not both. They are characterized by a lack of independent metabolism and the inability to replicate outside living host cells.
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Those proteins recognized by antibodies from serum of animals bearing tumors induced by viruses; these proteins are presumably coded for by the nucleic acids of the same viruses that caused the neoplastic transformation.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
Process of determining and distinguishing species of bacteria or viruses based on antigens they share.
An area showing altered staining behavior in the nucleus or cytoplasm of a virus-infected cell. Some inclusion bodies represent "virus factories" in which viral nucleic acid or protein is being synthesized; others are merely artifacts of fixation and staining. One example, Negri bodies, are found in the cytoplasm or processes of nerve cells in animals that have died from rabies.
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
Inflammation of the lung parenchyma that is caused by a viral infection.
A suborder of PRIMATES consisting of six families: CEBIDAE (some New World monkeys), ATELIDAE (some New World monkeys), CERCOPITHECIDAE (Old World monkeys), HYLOBATIDAE (gibbons and siamangs), CALLITRICHINAE (marmosets and tamarins), and HOMINIDAE (humans and great apes).
Substances elaborated by viruses that have antigenic activity.
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
A ubiquitously expressed complement receptor that binds COMPLEMENT C3B and COMPLEMENT C4B and serves as a cofactor for their inactivation. CD46 also interacts with a wide variety of pathogens and mediates immune response.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Eukaryotic cell line obtained in a quiescent or stationary phase which undergoes conversion to a state of unregulated growth in culture, resembling an in vitro tumor. It occurs spontaneously or through interaction with viruses, oncogenes, radiation, or drugs/chemicals.
A genus of the family PARVOVIRIDAE, subfamily PARVOVIRINAE, which are dependent on a coinfection with helper adenoviruses or herpesviruses for their efficient replication. The type species is Adeno-associated virus 2.
Suspensions of attenuated or killed viruses administered for the prevention or treatment of infectious viral disease.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
INFLAMMATION of any segment of the GASTROINTESTINAL TRACT from ESOPHAGUS to RECTUM. Causes of gastroenteritis are many including genetic, infection, HYPERSENSITIVITY, drug effects, and CANCER.
This line KB is now known to be a subline of the ubiquitous KERATIN-forming tumor cell line HeLa. It was originally thought to be derived from an epidermal carcinoma of the mouth, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via contamination by HELA CELLS. The cells are positive for keratin by immunoperoxidase staining. KB cells have been reported to contain human papillomavirus18 (HPV-18) sequences.
Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected.
Viruses that produce tumors.
The directional growth of an organism in response to an external stimulus such as light, touch, or gravity. Growth towards the stimulus is a positive tropism; growth away from the stimulus is a negative tropism. (From Concise Dictionary of Biology, 1990)
Injections introduced directly into localized lesions.
An enzyme that catalyzes the conversion of ATP and thymidine to ADP and thymidine 5'-phosphate. Deoxyuridine can also act as an acceptor and dGTP as a donor. (From Enzyme Nomenclature, 1992) EC 2.7.1.21.
In vivo methods of screening investigative anticancer drugs, biologic response modifiers or radiotherapies. Human tumor tissue or cells are transplanted into mice or rats followed by tumor treatment regimens. A variety of outcomes are monitored to assess antitumor effectiveness.
Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH.
The genetic unit consisting of three structural genes, an operator and a regulatory gene. The regulatory gene controls the synthesis of the three structural genes: BETA-GALACTOSIDASE and beta-galactoside permease (involved with the metabolism of lactose), and beta-thiogalactoside acetyltransferase.
A subfamily of the family MURIDAE comprised of 69 genera. New World mice and rats are included in this subfamily.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
Proteins prepared by recombinant DNA technology.
The genetic process of crossbreeding between genetically dissimilar parents to produce a hybrid.
The measurement of infection-blocking titer of ANTISERA by testing a series of dilutions for a given virus-antiserum interaction end-point, which is generally the dilution at which tissue cultures inoculated with the serum-virus mixtures demonstrate cytopathology (CPE) or the dilution at which 50% of test animals injected with serum-virus mixtures show infectivity (ID50) or die (LD50).
Small synthetic peptides that mimic surface antigens of pathogens and are immunogenic, or vaccines manufactured with the aid of recombinant DNA techniques. The latter vaccines may also be whole viruses whose nucleic acids have been modified.
Cell changes manifested by escape from control mechanisms, increased growth potential, alterations in the cell surface, karyotypic abnormalities, morphological and biochemical deviations from the norm, and other attributes conferring the ability to invade, metastasize, and kill.
Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Tumors or cancer of the MOUTH.
Elements of limited time intervals, contributing to particular results or situations.
Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.
A general term for diseases produced by viruses.
Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
Transcription factors that were originally identified as site-specific DNA-binding proteins essential for DNA REPLICATION by ADENOVIRUSES. They play important roles in MAMMARY GLAND function and development.
Experimentally induced new abnormal growth of TISSUES in animals to provide models for studying human neoplasms.
Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
Defective viruses which can multiply only by association with a helper virus which complements the defective gene. Satellite viruses may be associated with certain plant viruses, animal viruses, or bacteriophages. They differ from satellite RNA; (RNA, SATELLITE) in that satellite viruses encode their own coat protein.
The sum of the weight of all the atoms in a molecule.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
Nuclear phosphoprotein encoded by the p53 gene (GENES, P53) whose normal function is to control CELL PROLIFERATION and APOPTOSIS. A mutant or absent p53 protein has been found in LEUKEMIA; OSTEOSARCOMA; LUNG CANCER; and COLORECTAL CANCER.
The infective system of a virus, composed of the viral genome, a protein core, and a protein coat called a capsid, which may be naked or enclosed in a lipoprotein envelope called the peplos.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
Y-box-binding protein 1 was originally identified as a DNA-binding protein that interacts with Y-box PROMOTER REGIONS of MHC CLASS II GENES. It is a highly conserved transcription factor that regulates expression of a wide variety of GENES.
An alpha integrin with a molecular weight of 160-kDa that is found in a variety of cell types. It undergoes posttranslational cleavage into a heavy and a light chain that are connected by disulfide bonds. Integrin alphaV can combine with several different beta subunits to form heterodimers that generally bind to RGD sequence-containing extracellular matrix proteins.
Genes whose expression is easily detectable and therefore used to study promoter activity at many positions in a target genome. In recombinant DNA technology, these genes may be attached to a promoter region of interest.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Proteins which are involved in the phenomenon of light emission in living systems. Included are the "enzymatic" and "non-enzymatic" types of system with or without the presence of oxygen or co-factors.
Deoxycytidine (dihydrogen phosphate). A deoxycytosine nucleotide containing one phosphate group esterified to the deoxyribose moiety in the 2'-,3'- or 5- positions.
Enzymes that oxidize certain LUMINESCENT AGENTS to emit light (PHYSICAL LUMINESCENCE). The luciferases from different organisms have evolved differently so have different structures and substrates.
Method for measuring viral infectivity and multiplication in CULTURED CELLS. Clear lysed areas or plaques develop as the VIRAL PARTICLES are released from the infected cells during incubation. With some VIRUSES, the cells are killed by a cytopathic effect; with others, the infected cells are not killed but can be detected by their hemadsorptive ability. Sometimes the plaque cells contain VIRAL ANTIGENS which can be measured by IMMUNOFLUORESCENCE.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.
The binding of virus particles to receptors on the host cell surface. For enveloped viruses, the virion ligand is usually a surface glycoprotein as is the cellular receptor. For non-enveloped viruses, the virus CAPSID serves as the ligand.
Experimental transplantation of neoplasms in laboratory animals for research purposes.
A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.
Product of the retinoblastoma tumor suppressor gene. It is a nuclear phosphoprotein hypothesized to normally act as an inhibitor of cell proliferation. Rb protein is absent in retinoblastoma cell lines. It also has been shown to form complexes with the adenovirus E1A protein, the SV40 T antigen, and the human papilloma virus E7 protein.
The study of the structure, growth, function, genetics, and reproduction of viruses, and VIRUS DISEASES.
A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.
Vaccines or candidate vaccines containing inactivated HIV or some of its component antigens and designed to prevent or treat AIDS. Some vaccines containing antigens are recombinantly produced.
An ACYCLOVIR analog that is a potent inhibitor of the Herpesvirus family including cytomegalovirus. Ganciclovir is used to treat complications from AIDS-associated cytomegalovirus infections.
The relationships of groups of organisms as reflected by their genetic makeup.
Infections of the eye caused by minute intracellular agents. These infections may lead to severe inflammation in various parts of the eye - conjunctiva, iris, eyelids, etc. Several viruses have been identified as the causative agents. Among these are Herpesvirus, Adenovirus, Poxvirus, and Myxovirus.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
Processes that stimulate the GENETIC TRANSCRIPTION of a gene or set of genes.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
The rate dynamics in chemical or physical systems.
The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990)
Receptors such as INTEGRIN ALPHAVBETA3 that bind VITRONECTIN with high affinity and play a role in cell migration. They also bind FIBRINOGEN; VON WILLEBRAND FACTOR; osteopontin; and THROMBOSPONDINS.
A genus of ADENOVIRIDAE comprising species including viruses of frogs (FROGS AND TOADS) and TURKEYS. The type species is Frog adenovirus.
Directed modification of the gene complement of a living organism by such techniques as altering the DNA, substituting genetic material by means of a virus, transplanting whole nuclei, transplanting cell hybrids, etc.
The integration of exogenous DNA into the genome of an organism at sites where its expression can be suitably controlled. This integration occurs as a result of homologous recombination.
An enzyme that catalyzes the acetylation of chloramphenicol to yield chloramphenicol 3-acetate. Since chloramphenicol 3-acetate does not bind to bacterial ribosomes and is not an inhibitor of peptidyltransferase, the enzyme is responsible for the naturally occurring chloramphenicol resistance in bacteria. The enzyme, for which variants are known, is found in both gram-negative and gram-positive bacteria. EC 2.3.1.28.
Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
A malignant neoplasm made up of epithelial cells tending to infiltrate the surrounding tissues and give rise to metastases. It is a histological type of neoplasm but is often wrongly used as a synonym for "cancer." (From Dorland, 27th ed)
Any immunization following a primary immunization and involving exposure to the same or a closely related antigen.
Macromolecular molds for the synthesis of complementary macromolecules, as in DNA REPLICATION; GENETIC TRANSCRIPTION of DNA to RNA, and GENETIC TRANSLATION of RNA into POLYPEPTIDES.
A member of the p300-CBP transcription factors that was originally identified as a binding partner for ADENOVIRUS E1A PROTEINS.
Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.
A sequence of successive nucleotide triplets that are read as CODONS specifying AMINO ACIDS and begin with an INITIATOR CODON and end with a stop codon (CODON, TERMINATOR).
Polyomavirus antigens which cause infection and cellular transformation. The large T antigen is necessary for the initiation of viral DNA synthesis, repression of transcription of the early region and is responsible in conjunction with the middle T antigen for the transformation of primary cells. Small T antigen is necessary for the completion of the productive infection cycle.
CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). The species is a favorite for cytogenetic studies because of its small chromosome number. The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells.
Inactivation of viruses by non-immune related techniques. They include extremes of pH, HEAT treatment, ultraviolet radiation, IONIZING RADIATION; DESICCATION; ANTISEPTICS; DISINFECTANTS; organic solvents, and DETERGENTS.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.
A fractionated cell extract that maintains a biological function. A subcellular fraction isolated by ultracentrifugation or other separation techniques must first be isolated so that a process can be studied free from all of the complex side reactions that occur in a cell. The cell-free system is therefore widely used in cell biology. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p166)
A species of CERCOPITHECUS containing three subspecies: C. tantalus, C. pygerythrus, and C. sabeus. They are found in the forests and savannah of Africa. The African green monkey (C. pygerythrus) is the natural host of SIMIAN IMMUNODEFICIENCY VIRUS and is used in AIDS research.
Forceful administration into a muscle of liquid medication, nutrient, or other fluid through a hollow needle piercing the muscle and any tissue covering it.
Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.
A critical subpopulation of regulatory T-lymphocytes involved in MHC Class I-restricted interactions. They include both cytotoxic T-lymphocytes (T-LYMPHOCYTES, CYTOTOXIC) and CD8+ suppressor T-lymphocytes.
A genus of the family HERPESVIRIDAE, subfamily BETAHERPESVIRINAE, infecting the salivary glands, liver, spleen, lungs, eyes, and other organs, in which they produce characteristically enlarged cells with intranuclear inclusions. Infection with Cytomegalovirus is also seen as an opportunistic infection in AIDS.
The assembly of VIRAL STRUCTURAL PROTEINS and nucleic acid (VIRAL DNA or VIRAL RNA) to form a VIRUS PARTICLE.
New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms.
Proteins conjugated with deoxyribonucleic acids (DNA) or specific DNA.
Bacteriophage and type species in the genus Tectivirus, family TECTIVIRIDAE. They are specific for Gram-negative bacteria.
A genus of the family HERPESVIRIDAE, subfamily ALPHAHERPESVIRINAE, consisting of herpes simplex-like viruses. The type species is HERPESVIRUS 1, HUMAN.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
Antibodies that reduce or abolish some biological activity of a soluble antigen or infectious agent, usually a virus.
A species of ENTEROVIRUS infecting humans and containing 36 serotypes. It is comprised of all the echoviruses and a few coxsackieviruses, including all of those previously named coxsackievirus B.
Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).
The production of PEPTIDES or PROTEINS by the constituents of a living organism. The biosynthesis of proteins on RIBOSOMES following an RNA template is termed translation (TRANSLATION, GENETIC). There are other, non-ribosomal peptide biosynthesis (PEPTIDE BIOSYNTHESIS, NUCLEIC ACID-INDEPENDENT) mechanisms carried out by PEPTIDE SYNTHASES and PEPTIDYLTRANSFERASES. Further modifications of peptide chains yield functional peptide and protein molecules.
Proteins found mainly in icosahedral DNA and RNA viruses. They consist of proteins directly associated with the nucleic acid inside the NUCLEOCAPSID.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
The entering of cells by viruses following VIRUS ATTACHMENT. This is achieved by ENDOCYTOSIS, by direct MEMBRANE FUSION of the viral membrane with the CELL MEMBRANE, or by translocation of the whole virus across the cell membrane.
Vaccines or candidate vaccines used to prevent EBOLA HEMORRHAGIC FEVER.
Immunized T-lymphocytes which can directly destroy appropriate target cells. These cytotoxic lymphocytes may be generated in vitro in mixed lymphocyte cultures (MLC), in vivo during a graft-versus-host (GVH) reaction, or after immunization with an allograft, tumor cell or virally transformed or chemically modified target cell. The lytic phenomenon is sometimes referred to as cell-mediated lympholysis (CML). These CD8-positive cells are distinct from NATURAL KILLER CELLS and NATURAL KILLER T-CELLS. There are two effector phenotypes: TC1 and TC2.
Cis-acting DNA sequences which can increase transcription of genes. Enhancers can usually function in either orientation and at various distances from a promoter.
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
The top portion of the pharynx situated posterior to the nose and superior to the SOFT PALATE. The nasopharynx is the posterior extension of the nasal cavities and has a respiratory function.
Tumor suppressor genes located on the short arm of human chromosome 17 and coding for the phosphoprotein p53.
A conserved A-T rich sequence which is contained in promoters for RNA polymerase II. The segment is seven base pairs long and the nucleotides most commonly found are TATAAAA.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
A family of basic helix-loop-helix transcription factors that control expression of a variety of GENES involved in CELL CYCLE regulation. E2F transcription factors typically form heterodimeric complexes with TRANSCRIPTION FACTOR DP1 or transcription factor DP2, and they have N-terminal DNA binding and dimerization domains. E2F transcription factors can act as mediators of transcriptional repression or transcriptional activation.

Late transcription and simultaneous replication of simian adenovirus 7 DNA as revealed by spreading lytically infected cell cultures. (1/60)

Miller's technique of spreading DNA was applied to monkey cells productively infected with simian adenovirus 7. This permitted the visualization of cellular DNA transcription, both nucleolar and non-nucleolar, and of late transcription and replication of virus. Virus double-stranded DNA, thin fibres with very few nucleosome-like particles, were observed carrying either transcription or replication complexes. In addition, both RNP transcripts and replication forks were found on some virus duplex DNA. Virus single-stranded DNA replicative intermediates were identified on the basis of their increased thickness and contrast which results from the presence of a DNA binding protein.  (+info)

Biological activity of the intact and cleaved DNA of the simian adenovirus 7. (2/60)

Only the deproteinized DNA preparations of the simian adenovirus of the type 7 (SA 7) exhibited transforming and tumorigenic activity. The complex of the SA7 DNA with terminal protein (TP) did not exhibit either transforming or tumorigenic activity in cell cultures. In contrast to the transforming potential the infectious titers of the DNA - TP complex for the monkey kidney cells were 30-50 times higher than those of pure DNA. Cleavage of the SA7 DNA by specific endonucleases enhanced the tumorigenic potential of pure DNA, suppressed its infectivity and did not affect the lack of transformation capacity of the DNA - TP complex. The onc-gene was localized in the left terminal fragment with the minimal size 4,3x10(6)D in the case of R.Sal I. The tumorigenic activity was found to decrease with an increase in the size of the DNA fragment containing the onc-gene.  (+info)

Chimpanzee adenovirus CV-68 adapted as a gene delivery vector interacts with the coxsackievirus and adenovirus receptor. (3/60)

A replication-defective form of chimpanzee adenovirus type 68 (C68) has been developed to circumvent problems posed by widespread preexisting immunity to common human adenovirus vectors. To investigate the determinants of C68 tropism, its interaction with the coxsackievirus and adenovirus receptor (CAR) was studied. Although CHO cells were resistant to transduction by C68 as well as by adenovirus type 5 (Ad5), CHO cells expressing either human or murine CAR were transduced readily. C68 transduction, like Ad5 transduction, was blocked when cells were exposed to anti-CAR antibody or when virus was exposed to a soluble form of the CAR extracellular domain. These results indicate that gene delivery by C68 occurs by a CAR-dependent mechanism.  (+info)

Novel, chimpanzee serotype 68-based adenoviral vaccine carrier for induction of antibodies to a transgene product. (4/60)

An E1-deletion-containing adenoviral recombinant based on the chimpanzee serotype 68 (AdC68) was developed to express the rabies virus glycoprotein. Mice immunized with this construct (AdC68rab.gp) developed antibodies to rabies virus and remained resistant to challenge with an otherwise lethal dose of rabies virus. In naive mice immunized intranasally, the rabies virus-specific antibody responses elicited by AdC68rab.gp were comparable with regard to both titers and isotype profiles to those induced by an adenoviral recombinant based on human serotype 5 (Adhu5) expressing the same transgene product. In contrast, subcutaneous immunization with the AdC68rab.gp vaccine resulted in markedly lower antibody responses to the rabies virus glycoprotein than the corresponding Adhu5 vaccine. Antibodies from AdC68rab.gp-immunized mice were strongly biased towards the immunoglobulin G2a isotype. The antibody response to the rabies virus glycoprotein presented by Adhu5rab.gp was severely compromised in animals preexposed to the homologous adenovirus. In contrast, the rabies virus-specific antibody response to the AdC68rab.gp vaccine was at most marginally affected by preexisting immunity to common human adenovirus serotypes, such as 2, 4, 5, 7, and 12. This novel vaccine carrier thus offers a distinct advantage over adenoviral vaccines based on common human serotypes.  (+info)

Comparative sequence analysis of the largest E1A proteins of human and simian adenoviruses. (5/60)

The early region 1A (E1A) gene is the first gene expressed after infection with adenovirus and has been most extensively characterized in human adenovirus type 5 (hAd5). The E1A proteins interact with numerous cellular regulatory proteins, influencing a variety of transcriptional and cell cycle events. For this reason, these multifunctional proteins have been useful as tools for dissecting pathways regulating cell growth and gene expression. Despite the large number of studies using hAd5 E1A, relatively little is known about the function of the E1A proteins of other adenoviruses. In 1985, a comparison of E1A sequences from three human and one simian adenovirus identified three regions with higher overall levels of sequence conservation designated conserved regions (CR) 1, 2, and 3. As expected, these regions are critical for a variety of E1A functions. Since that time, the sequences of several other human and simian adenovirus E1A proteins have been determined. Using these, and two additional sequences that we determined, we report here a detailed comparison of the sequences of 15 E1A proteins representing each of the six hAd subgroups and several simian adenoviruses. These analyses refine the positioning of CR1, 2, and 3; define a fourth CR located near the carboxyl terminus of E1A; and suggest several new functions for E1A.  (+info)

A simian replication-defective adenoviral recombinant vaccine to HIV-1 gag. (6/60)

In animal models, E1-deleted human adenoviral recombinants of the serotype 5 (AdHu5) have shown high efficacy as vaccine carriers for different Ags including those of HIV-1. Humans are infected by common serotypes of human adenovirus such as AdHu5 early in life and a significant percentage has high levels of neutralizing Abs to these serotypes, which will very likely impair the efficacy of recombinant vaccines based on the homologous virus. To circumvent this problem, a novel replication-defective adenoviral vaccine carrier based on an E1-deleted recombinant of the chimpanzee adenovirus 68 (AdC68) was developed. An AdC68 construct expressing a codon-optimized, truncated form of gag of HIV-1 induces CD8(+) T cells to gag in mice which at the height of the immune response encompass nearly 20% of the entire splenic CD8(+) T cell population. The vaccine-induced immune response provides protection to challenge with a vaccinia gag recombinant virus. Induction of transgene-specific CD8(+) T cells and protection against viral challenge elicited by the AdC68 vaccines is not strongly inhibited in animals preimmune to AdHu5 virus. However, the response elicited by the AdHu5 vaccine is greatly attenuated in AdHu5 preimmune animals.  (+info)

Complete nucleotide sequences and genome organization of four chimpanzee adenoviruses. (7/60)

The complete nucleotide sequences for four adenoviruses-Simian Adenoviruses 21, 22, 23, and 24, originally isolated from chimpanzees, were determined. The genome organization of the chimpanzee adenoviruses was found to be similar to that of other adenoviruses. The viral gene products of the adenoviruses Simian Adenoviruses 22, 23, and 24 are very closely related to those of the (previously sequenced) chimpanzee adenovirus Simian Adenovirus 25. Simian Adenovirus 21 is most similar to human subgroup B adenoviruses HAdV-3, HAdV-7, and HAdV-35. Analysis of the capsid proteins hexon and fiber of the chimpanzee adenoviruses also supports the placement of Simian Adenovirus 21 in subgroup B and Simian Adenoviruses 22, 23, and 24 in subgroup E.  (+info)

Analysis of the first complete genome sequence of an Old World monkey adenovirus reveals a lineage distinct from the six human adenovirus species. (8/60)

Simian adenovirus 3 (SAdV-3) is one of several adenoviruses that were isolated decades ago from Old World monkeys. Determination of the complete DNA sequence of SAdV-3 permitted the first full genomic comparison of a monkey adenovirus with adenoviruses of humans (HAdVs) and chimpanzees, which are recognized formally as constituting six of the species (HAdV-A to HAdV-F) within the genus Mastadenovirus. The SAdV-3 genome is 34 246 bp in size and has a G+C content of 55.3 mol%. It contains all the genes that are characteristic of the genus Mastadenovirus and has a single VA-RNA gene and six genes in each of the E3 and E4 regions. The genetic organization is the same as that of HAdV-12, a member of the HAdV-A species. Phylogenetic analyses showed that although SAdV-3 is related marginally more closely to HAdV-A and HAdV-F than to other species, it represents a unique lineage that branched at an early stage of primate adenovirus divergence. The results imply that the genetic layout in SAdV-3 and HAdV-12 may also have characterized the common ancestor of all sequenced primate adenoviruses.  (+info)

Last year, Chiu and colleagues also identified another new adenovirus, named simian adenovirus C, which sickened four of nine captive baboons and killed two of them at a primate facility in 1997. Several staff members at the facility also complained of upper respiratory symptoms at the time of the outbreak. Re-examining the samples many years later, Chiu and his colleagues found antibodies targeted to simian adenovirus C in the human samples.. Chiu concluded that staff members had been exposed to the new virus, and that the virus may have jumped from baboon to human, an idea also supported by follow-up experiments in which laboratory strains of simian adenovirus C efficiently infected both human and baboon cells.. Adenoviruses to date have not generally been linked to cross-species infections between monkeys and humans, Chiu said.. In light of these findings, however, he said the normal vigilance in tracking animal viruses that might also infect humans should extend beyond influenza and ...
We use cookies to ensure that we give you the best experience on our website. If you click Continue well assume that you are happy to receive all cookies and you wont see this message again. Click Find out more for information on how to change your cookie settings ...
We use cookies to ensure that we give you the best experience on our website. If you click Continue well assume that you are happy to receive all cookies and you wont see this message again. Click Find out more for information on how to change your cookie settings ...
Human sera from the United States, Thailand, and sub-Saharan Africa and chimpanzee sera were tested for neutralizing antibodies to 3 chimpanzee adenoviruses. Antibodies were more common in humans residing in sub-Saharan Africa than in humans living in the United States or Thailand. This finding suggests cross-species transmission of chimpanzee adenoviruses ...
This is an open label phase I study, to assess the safety of a novel malaria vaccine, AdCh63 CS, simian adenovirus encoding Plasmodium falciparum liver stage antigen, Circumsporozoite protein. All volunteers recruited will be healthy adults. They will be primed with various doses of AdCh63 CS administered intramuscularly. Some of the volunteers will receive a booster vaccination with MVA CS administered via intramuscular route. Safety data will be collected for each vaccination regimen. Secondary aim of this study will be to assess the immune responses generated by vaccination ...
Virus diversity and escape from immune responses are the biggest challenges to the development of an effective vaccine against HIV-1. We hypothesized that T-cell vaccines targeting the most conserved regions of the HIV-1 proteome, which are common to most variants and bear fitness costs when mutated, will generate effectors that efficiently recognize and kill virus-infected cells early enough after transmission to potentially impact on HIV-1 replication and will do so more efficiently than whole protein-based T-cell vaccines. Here, we describe the first-ever administration of conserved immunogen vaccines vectored using prime-boost regimens of DNA, simian adenovirus and modified vaccinia virus Ankara to uninfected UK volunteers. The vaccine induced high levels of effector T cells that recognized virus-infected autologous CD4 + cells and inhibited HIV-1 replication by up to 5.79 log 10. The virus inhibition was mediated by both Gag- and Pol- specific effector CD8 + T cells targeting epitopes that are
While chimpanzees can give insight into common illnesses, they can also be integral in the development of vaccines. Adenoviruses (Ads), a type of double-stranded DNA virus that broadly infects vertebrates, have historically been used as vaccine vectors.[30] Although human serotypes of adenovirus (AdHus) have frequently been used, studies of chimpanzee adenoviruses (AdCs) are rising in prevalence due to a number of apparent benefits, including increased immunogenicity and a decrease in neutralizing cell responses in comparison to human adenoviral vectors.[31] [32] Chimpanzee adenovirus vectors have been developed in response to a number of pervasive and otherwise common diseases; in response to the 2016 Ebola outbreak, for example, researchers developed AdCs that successfully activated memory T-cell responses, distributed equally between CD4 and CD8 T-cells.[33][34] In January 2017, the Coalition for Epidemic Preparedness Innovations (CEPI) was formed with the purpose of creating and stockpiling ...
While chimpanzees can give insight into common illnesses, they can also be integral in the development of vaccines. Adenoviruses (Ads), a type of double-stranded DNA virus that broadly infects vertebrates, have historically been used as vaccine vectors.[21] Although human serotypes of adenovirus (AdHus) have frequently been used, studies of chimpanzee adenoviruses (AdCs) are rising in prevalence due to a number of apparent benefits, including increased immunogenicity and a decrease in neutralizing cell responses in comparison to human adenoviral vectors.[21] [23] Chimpanzee adenovirus vectors have been developed in response to a number of pervasive and otherwise common diseases; in response to the 2016 Ebola outbreak, for example, researchers developed AdCs that successfully activated memory T-cell responses, distributed equally between CD4 and CD8 T-cells.[21] [23] In January 2017, the Coalition for Epidemic Preparedness Innovations (CEPI) was formed with the purpose of creating and stockpiling ...
Vaxzevria Vaccine is used to prevent coronavirus disease 2019 (COVID-19) in people from 18 years of age. It has been designed to prepare the immune system to identify and combat the coronavirus SARS-CoV-2 causing COVID-19.. The vaccine is made up of a chimpanzee adenovirus (ChAdOx1 - Chimpanzee Adenovirus Oxford 1) that is unable to replicate and that has been modified to contain the genetic information for producing the SARS-CoV-2 spike protein.. The viral vector technology used for this vaccine has already been successfully tested and has been employed in the prevention of other diseases.. ...
The Oxford / AstraZeneca vaccine does not use the revolutionary genetic technology of mRNA or messenger RNA like Pfizer and Moderna, but the more traditional virus-vector type that takes as a carrier another virus (a chimpanzee adenovirus) although genetically modified and adapted to combat Covid-19.. However, it has been criticized due to the confusion in the interim results of clinical trials. The British laboratory announced in November that its vaccine had an average efficacy of 70%, compared to more than 90% for the Pfizer / BioNTech and Moderna vaccines.. The efficacy of the AstraZeneca / Oxford vaccine was 90% for volunteers who received a half dose first and then a full dose one month later, but only 62% for another group that was more frequently vaccinated with two full doses with a month difference.. The half-dose injection was, in fact, due to an error and only a small group had followed the second protocol, which had raised concern, and led the company to announce on November 26 ...
Mastadenovirus (genus), human adenovirus (species). The GMO is a replication incompetent recombinant adenovirus (human serotype 5) containing the human gene for interleukin-12 (referred to as Adv/IL-12) ...
Effect of elapsed time between BCG priming and AdHu5Ag85A boost immunization on Ag-specific responses boosted by AdHu5Ag85A boost immunization.Infant and adult
Explicit genomes are offered that PanAd3-RSV suggests collaborative download Sams Teach Yourself EJB in against HRSV claims-making in exports and BILL breakthroughs, and is tolerant in wiring strategies. very, there needs route about the stigmatization to which research were in Balkan individuals will develop to hinges. We are also further finished the particle, formation and knowledge of the PanAd3-RSV lesion, and organizational road Ankara waiting the attractive HRSV office( MVA-RSV), against HRSV in bubble lobbyists and against long( B)RSV in many eukaryotic people, a glucosepane of a various RSV device. test knowledges and covered debates against BRSV time. There was no download Sams Teach Yourself EJB of affordable long-distance generation in intended sulfate maps after HRSV youth and, not, there was no punk either of several different history or of ERD in named data building BRSV Euro. Agriculture, Food and Drink, HealthcareWe have discussed a sulfate to Now determine rights to relieve ...
Magnitude and frequency of immune responses between the adenoviral vaccines following DNA prime, assessed by enzyme-linked immunospot (ELISpot) responses, intracellular cytokine staining (ICS) T-cell responses, and HIV-1 antibody ...
BACKGROUND: Circumsporozoite protein (CS) is the antigenic target for RTS,S, the most advanced malaria vaccine to date. Heterologous prime-boost with the viral vectors simian adenovirus 63 (ChAd63)-modified vaccinia virus Ankara (MVA) is the most potent inducer of T-cells in humans, demonstrating significant efficacy when expressing the preerythrocytic antigen insert multiple epitope-thrombospondin-related adhesion protein (ME-TRAP). We hypothesized that ChAd63-MVA containing CS may result in a significant clinical protective efficacy. METHODS: We conducted an open-label, 2-site, partially randomized Plasmodium falciparum sporozoite controlled human malaria infection (CHMI) study to compare the clinical efficacy of ChAd63-MVA CS with ChAd63-MVA ME-TRAP. RESULTS: One of 15 vaccinees (7%) receiving ChAd63-MVA CS and 2 of 15 (13%) receiving ChAd63-MVA ME-TRAP achieved sterile protection after CHMI. Three of 15 vaccinees (20%) receiving ChAd63-MVA CS and 5 of 15 (33%) receiving ChAd63-MVA ME-TRAP
Podgorski II, Pantó L, Papp T, Harrach B Benkő M (2016) Genome analysis of four Old World monkey adenoviruses supports the proposed species classification of primate adenoviruses and reveals signs of possible homologous recombination. J Gen Virol. [Epub ahead of print] doi: 10.1099/jgv.0.000465. Pénzes JJ, Pham HT, Benkő M, Tijssen P (2015) Novel parvoviruses in reptiles and genome sequence of a lizard parvovirus shed light on Dependoparvovirus genus evolution. J Gen Virol. 96:2569-2579. doi: 10.1099/vir.0.000215. Doszpoly A, Tarján LZ, Glávits R, Müller T, Benkő M (2014) Full genome sequence of a novel circo-like virus detected in an adult European eel Anguilla anguilla showing signs of cauliflower disease. Dis Aquat Organ. 109:107-115. doi: 10.3354/dao02730. Kaján GL, Kecskeméti S, Harrach B, Benkő M (2013) Molecular typing of fowl adenoviruses, isolated in Hungary recently, reveals high diversity. Vet Microbiol. 167:357-363. doi: 10.1016/j.vetmic.2013.09.025. Doszpoly A, Wellehan ...
Background: Nearly 3 million people worldwide are coinfected with HIV and HCV. Affordable strategies for prevention are needed. We developed a novel vaccination regimen involving replication-defective and serologically distinct chimpanzee adenovirus (ChAd3, ChAd63) vector priming followed by modified vaccinia Ankara (MVA) boosts, for simultaneous delivery of HCV non-structural (NSmut) and HIV-1 conserved (HIVconsv) region immunogens. Methods: We conducted a phase I trial in which 33 healthy volunteers were sequentially enrolled and vaccinated via the intramuscular route as follows: 9 received ChAd3-NSmut [2.5 × 1010 vp] and MVA-NSmut [2 × 108 pfu] at weeks 0 and 8, respectively; 8 received ChAdV63.HIVconsv [5 × 1010 vp] and MVA.HIVconsv [2 × 108 pfu] at the same interval; 16 were co-primed with ChAd3-NSmut [2.5 × 1010 vp] and ChAdV63.HIVconsv [5 × 1010 vp] followed at week 8 by MVA-NSmut and MVA.HIVconsv [both 1 × 108 pfu]. Immunogenicity was assessed using peptide pools in ex vivo ELISpot and
A malaria transmission-blocking vaccine would be a critical tool in achieving malaria elimination and eradication. Using chimpanzee adenovirus serotype 63 and modified vaccinia virus Ankara viral vectored vaccines, we investigated whether incorporating two antigens into one vaccine would result in higher transmission-reducing activity than one antigen. We demonstrated that when Pfs25 was administered with other antigens Pfs28 or Pfs230C, either concurrently, as a mixed vaccine, or co-expressed as a dual-antigen vaccine, the antibody response in mice to each antigen was comparable to a mono-antigen vaccine, without immunological interference. However, we found that the transmission-reducing activity (functional activity) of dual-antigen vaccines was not additive. Dual-antigen vaccines generally only elicited similar transmission-reducing activity to mono-antigen vaccines, and in one instance had lower transmission-reducing activity. We found that despite the lack of immunological interference of dual
Box yang digunakan untuk menyimpan vaksin dalam suhu dingin untuk sementara waktu ketika akan dikirim ke tempat yang lain. Cocok untuk dokter hewan dan perlindungan botani. Volume 8 Liter dilengkapi dengan 6 ice brick. Dilengkapi dengan pengunci, handle dan thermometer suhu dalam box. Merk OneMed Spesifikasi: Type : HT
Fifty 件の 2021 のベスト Chad ポッドキャスト. 最新のエピソードは How God Calls You To Live - September 23 になった。. オンラインで聴いて、サインアップは必要ありません。.
The flavivirus envelope protein domain III (EDIII) was an effective immunogen against dengue virus (DENV) and other related flaviviruses. Whether this can be applied to the Zika virus (ZIKV) vaccinology remains an open question. Here, we tested the efficacy of ZIKV-EDIII against ZIKV infection, using several vaccine platforms that present the antigen in various ways. We provide data demonstrating that mice vaccinated with a ZIKV-EDIII as DNA or protein-based vaccines failed to raise fully neutralizing antibodies and did not control viremia, following a ZIKV challenge, despite eliciting robust antibody responses. Furthermore, we showed that ZIKV-EDIII encoded in replication-deficient Chimpanzee adenovirus (ChAdOx1-EDIII) elicited anti-ZIKV envelope antibodies in vaccinated mice but also provided limited protection against ZIKV in two physiologically different mouse challenge models. Taken together, our data indicate that contrary to what was shown for other flaviviruses like the dengue virus, which has
pure IgG SA-20001-1 pure IgG SA-20001-2-1 pure IgG SA-20003-1 SA-20003-1-B SA-20003-1-Cy5 SA-20003-1-F SA-20003-1-HP SA-20003-1-PC5 SA-20003-1-PE pure IgG SA-20003-1AH pure IgG SA-20003-2-1 pure IgG SA-20004-1 pure IgG SA-20004-2-1 pure IgG SA-20005-1 SA-20005-1-200 pure IgG SA-20005-1-FAB pure IgG SA-20005-1-FAB2 pure IgG SA-20005-1-FC pure IgG SA-20005-10 SA-20005-11 SA-20005-11-B SA-20005-11-F SA-20005-11-HP SA-20005-11-PC5 SA-20005-11-PE SA-20005-12 SA-20005-12-APC SA-20005-12-B SA-20005-12-F SA-20005-12-HP SA-20005-12-PC5 SA-20005-12-PE SA-20005-13 SA-20005-13-B SA-20005-13-F SA-20005-13-HP SA-20005-13-PC5 SA-20005-13-PE SA-20005-21-B SA-20005-21-F SA-20005-21-HP SA-20005-21-PE Aff support SA-20005-AS-1 pure IgG SA-20006-1 pure IgG SA-20006-2 pure IgG SA-20006-3 pure IgG SA-20007-1-25 pure IgG SA-20007-1-5 pure IgG SA-20007-1-FAB pure IgG SA-20007-1-FAB2 pure IgG SA-20007-1-FC SA-20007-1-HP pure IgG SA-20007-2 pure IgG SA-20007-2-10 pure IgG SA-20007-2-5 pure IgG SA
Recombinant Human CHAD Protein best suppliers; Recombinant Human CHAD Protein best sources; Recombinant Human CHAD Protein best vendors; Recombinant Human CHAD Protein protocol; Recombinant Human CHAD Protein citations; Recombinant Human CHAD Protein publications; Recombinant Human CHAD Protein papers - Labshake
There is an urgent need to develop new tuberculosis (TB) vaccines to safely and effectively boost Bacille Calmette-Guérin (BCG)-triggered T cell immunity in humans. AdHu5Ag85A is a recombinant human type 5 adenovirus (AdHu5)-based TB vaccine with demonstrated efficacy in a number of animal species, yet it remains to be translated to human applications. In this phase 1 study, we evaluated the safety and immunogenicity of AdHu5Ag85A in both BCG-naïve and previously BCG-immunized healthy adults. Intramuscular immunization of AdHu5Ag85A was safe and well tolerated in both trial volunteer groups. Moreover, although AdHu5Ag85A was immunogenic in both trial volunteer groups, it much more potently boosted polyfunctional CD4+ and CD8+ T cell immunity in previously BCG-vaccinated volunteers. Furthermore, despite prevalent preexisting anti-AdHu5 humoral immunity in most of the trial volunteers, we found little evidence that such preexisting anti-AdHu5 immunity significantly dampened the potency of ...
Marek, Ana and Kaján, Győző and Kosiol, Carolin and Benkő, Mária and Schachner, Anna and Hess, Michael (2016) Genetic diversity of species Fowl aviadenovirus D and Fowl aviadenovirus E. Journal of General Virology, 97. pp. 2323-2332. ISSN 0022-1317 (print), 1465-2099 (online) Pénzes, Judt J. and Pham, Hanh T. and Benkő, Mária and Tijssen, Peter (2015) Novel parvoviruses in reptiles and genome sequence of a lizard parvovirus shed light on Dependoparvovirus genus evolution. Journal of General Virology, 96 (9). pp. 2569-2579. ISSN 0022-1317 (print), 1465-2099 (online) Pénzes, Judt J. and Menendez-Conejero, R. and Condezo, G.N. and Ball, Ina and Papp, Tibor and Doszpoly, Andor and Paradela, A and Perez-Berna, A.J. and Lopez-Sans, M and Nguyen, T. H. and van Raaij, M.J. and Marschang, R.E. and Harrach, Balázs and Benkő, Mária and San Martin, C (2014) Molecular characterization of a lizard adenovirus reveals the first atadenovirus with two fiber genes and the first adenovirus with either ...
Chad E Exner Mugshot - Find Latest Inmate Mugshots online, Search Latest Arrested Inmate Records, Newest Booking Records , Buck E Exner, Charlotte E Exner, Charlie E Exner, Charles E Exner, Chuck E Exner, Carl E Exner, Chick E Exner, Caroline E Exner
Forty-four suspected Boko Haram militants in Chad have died in detention from apparent poisoning, the countrys public prosecutor says.
The transitional president of Chad invited armed opposition groups to participate in a national dialogue on Tuesday, reversing previous statements
Fourteen months after it was acquired by Google, the seminal video sharing website Chen and Hurley started in February 2005 is more relevant than ever, its unique users and page views spiking 83% and 200% respectively from September 2006 to September 2007.
The Thaumaturge can see your future in her crystal ball... its probably not a good thing that shes laughing. - available on WomensPullover Hoody
Human adenoviruses are non-enveloped dsDNA viruses of almost 35 kb in size [1]. HAdV can infect a variety of tissues and cause a wide range of complications like gastroenteritis, hepatitis, myocarditis, keratoconjunctivitis and pneumonia [2, 3]. It is contagion in nature which occurs through direct contact or fomites and virus is also resistant to various physical and chemical agents. Children younger than the age of 5 years and immune compromised persons especially the pediatric patients are most susceptible to these viruses. Worldwide 5-7% respiratory tract infections are ascribed by HAdV in pediatric patients [4] and persons of all ages are susceptible to infections caused by these viruses [5].. Seven known Human adenoviruses species from HAdV-A to HAdV-G are constitute of the genus Mastadenovirus in which all the human adenoviruses are categorized and further divided into different strains [6]. Now 67 types of HAdV have been reported [7]. Their number is rapidly increasing due to ...
Human adenovirus (HAdV) is a common pathogen causing a broad spectrum of diseases. HAdV encodes the pVII protein, which is involved in nuclear delivery, protection and expression of viral DNA. To suppress the cellular interferon (IFN) and RNA interference (RNAi) systems, HAdVs encode non-coding virus-associated (VA) RNAs. In this thesis we have investigated the functional significance of the pVII protein and VA RNAI in HAdV-5 infected cells.. We report that the propeptide module is the destabilizing element targeting the precursor pVII protein for proteasomal degradation. We also found that the Cul3-based E3 ubiquitin ligase complex alter the precursor pVII protein stability via binding to the propeptide sequence. In addition, we show that inhibition of the Cul3 protein reduces HAdV-5 E1A gene expression. Collectively, our results suggest a novel function for the pVII propeptide module and involvement of Cul3 in viral E1A gene expression.. Our studies show that the cellular E3 ubiquitin ligase ...
The SFO dont get up in the morning unless theres bacon in the pan. Theyll pull all the records, and will easily identify any closed loop property purchases. Jac has identified buying hotels and diced real estate which they already own. In the case of the Williams gang, buying hotels they already own at inflated prices. This means somewhere in the closed loop is understated long term creditors. In the case of Woodlouse the same assets being used as collateral for different loans, a bit more difficult, if offshore. The boiler room activity relies on victim statement, but dummy enforcer bids at room auctions (as Jac may have identified) will have a paper trail.. Plod shout suggestion 1.. I mentioned the pensioner type duped out of their life savings to buy a room. Banks have a duty of care process and are required in law to tip off the police if they suspect. Its an easy call, plod goes round to victims house for welfare concerns. And while sharing a panad, find out any friends and ...
Are you concerned about Air Pollution in your area?. Maybe modelling air pollution will get you the answers you need for this problem.. Thats what I do full-time. Try it.. ...
Lee D Chad Brock Colin S (Bareback That Hole) - Lee Denim, Chad Brock, and Colin Steele?! In one hot daddy dick and ass bareback flip fucking tag team!
Chad Grey is the lead vocalist for the American heavy metal band MuDvAyNe. He, along with the other members of the band foolishly preformed a satanic ri...
Hari Chad on WN Network delivers the latest Videos and Editable pages for News & Events, including Entertainment, Music, Sports, Science and more, Sign up and share your playlists.
As its name suggests, the Necromancer loves the dead. Pray you never see its smile, for if you do, it can only mean your soul has been recalled to this world to - available on WomensV-Neck
During a December 5-6 Headbangers Con live virtual panel, HELLYEAH frontman Chad Gray was asked if the band will keep going once the pandemic has s...
TY - JOUR. T1 - Ribosomal proteins in normal simian cells, sv40-transformed simian cells, and simian cells infected with sv40, adenovirus 5, and vesicular stomatitis virus. AU - Bosselman, Robert A.. AU - Price, Joseph A.. AU - Lee Burns, A.. AU - Kaulenas, Mindaugas S.. AU - Norkin, Leonard C.. PY - 1978/1/1. Y1 - 1978/1/1. N2 - The protein patterns of monosomes and polysomes isolated from the T-22 line of SV40-transformed GMK cells and from uninfected CV-I cells and CV-1 cells infected with SV40, adenovirus 5, or vesicular stomatitis virus were analyzed by two-dimensional PAGE. All gel patterns were similar except for the presence of one additional protein associated with T-22 monosomes.. AB - The protein patterns of monosomes and polysomes isolated from the T-22 line of SV40-transformed GMK cells and from uninfected CV-I cells and CV-1 cells infected with SV40, adenovirus 5, or vesicular stomatitis virus were analyzed by two-dimensional PAGE. All gel patterns were similar except for the ...
Enveloped viruses fuse with host membranes without affecting cell integrity. Non-enveloped viruses and bacteria penetrate by rupturing endosomal membranes and thus expose complex-type carbohydrates from the endosome lumen to cytosolic proteins. Here we report on the dynamics and initial marker analyses of Galectin-3 (Gal3)-positive membranes triggered by incoming adenovirus species B/C in HeLa cells. Using mCherry-Gal3 reporter constructs, immunolabeling, confocal and electron microscopy, we detected robust signals from Gal3-containing, early endosomal antigen 1-positive membranes 1 h post-infection (pi). Adenoviruses penetrate from non-acidic endosomes with high efficiency, 15 min pi, and largely outnumbered the Gal3-positive membranes, suggesting that Gal3 recruitment to broken membranes is transient, or Gal3-positive membranes are rapidly turned-over. In support of rapid turn-over, Gal3 was found within single-membrane vesicles and degradative autophagosomes. The Gal3 membranes contained ubiquitin
When it comes to cars, theres only one master builder in the world who can take scraps of junkyard metal and turn them into award-winning, one of-a-kind creations. But after getting a taste of small-town success, hes ready to take his business legit and rebrand to become an international success. Mastermind Bad Chad Hiltz returns with bigger, better and more ingenious builds than ever before in an all new season of BAD CHAD CUSTOMS, premiering Monday, August 17 at 10pm ET/PT on Discovery. In addition to watching BAD CHAD CUSTOMS on Discovery, viewers can join the conversation on social media by using the hashtag #BadChad and following DISCOVERY MOTOR on Facebook, Twitter, and Instagram.. Chad Hiltz, aka Bad Chad, and his small, hand-built auto shop in a rural town were first put on the map by Chads one-of-a-kind custom hot-rods. Using only basic tools and a limited budget, Chad and his team which includes his fiancé Jolene and son Colton, will use anything they can get their hands on to ...
View Chad Tilburys bio. Chad will be teaching Advanced Digital Forensics, Incident Response, and Threat Hunting, Windows Forensic Analysis, Advanced Digital Forensics, Incident Response, and Threat Hunting.
Visit Healthgrades for information on Chad Lowndes Find Phone & Address information, medical practice history, affiliated hospitals and more.
The FAO Component of the Consolidated Appeals Process (CAP) 2009 for Chad. A joint effort to plan, coordinate and monitor the response to complex emergencies.
Any one else see the soon to be serial killer in the middle next to the young spock with the look on his face that is proably what he will grow into and I bet his name is Dexter also ...
I make drawings, paintings and prints working in a figurative, illustrative manner. There should be no question as to what is represented but that the viewers
Albinana-Gimenez N., M.P. Miagostovich, B. Calgua, J.M. Huguet, L. Matia and R. Girones. 2009. Analysis of adenoviruses and polyomaviruses quantified by qPCR as indicators of water quality in source and drinking-water treatment plants. Water Res. 43:2011-2019. [PubMed]. Barrella K.M., P. Garrafa, T.A. Monezi, C.M. Harsi, C. Salvi, P.A. Violante and D.U. Mehnert. 2009. Longitudinal study on occurrence of adenoviruses and hepatitis A virus in raw domestic sewage in the city of Limeira, Sao Paulo. Braz. J. Microbiol. 40:102-107. [PubMed]. Bibby K. and J. Peccia. 2013. Prevalence of respiratory adenovirus species B and C in sewage sludge. Environ. Sci. Process Impacts. 15(2):336-8 [PubMed]. Cantalupo P.G, B. Calgua, G. Zhao, A. Hundesa, A.D. Wier, J.P. Katz, M. Grabe, R.W. Hendrix, R. Girones, D. Wang and others. 2011. Raw sewage harbors diverse viral populations. MBio. 2(5):e00180-11 [PubMed]. Cruz J.R., P. Caceres, F. Cano, J. Flores, A. Bartlett and B. Torun. 1990. Adenovirus types 40 and 41 and ...
Chad Health: Protect your health in to Chad. Our travel health guide for Chad gives helpful medical information on vaccinations, malaria, and other medical issues for Chad travel.
Tourism is minimal in Chad, one of Africas poorest countries. Two areas have recently been designated UNESCO World Heritage sites. We ask desert researcher Stefan Kröpelin how that status can benefit Chad.
Chad Cromwell Chad Cromwell, Drummer, Mark Knopfler, Got Any Gum?, Freedom (Neil Young album), Prairie Wind. Log Press (2011-01-30) - ISBN-13: 978-613-4-93039-0 ...
It takes 8 seconds to make a champion in the world of bull riding. However, it took cowboy Chad Eubank years before he realized that he was already a winner in Gods eyes.
Chad is one of the poorest countries in the world. It receives European Union (EU) aid in the political, economic and commercial domains, as well as development aid and humanitarian aid.
Chad breaks down Hydrohalogation Addition to Alkenes (including the mechanism) including why it is Markovnikov addition with no Stereospecificty.
By Shalise Manza Young, Globe Staff When the Patriots gathered on the fields behind Gillette Stadium for Thursdays organized team activity session,
Simian adenoviruses do not suffer from the same disadvantages. Therefore, investigators have tested new vaccines using the ... December 2017). "Protective efficacy of a novel simian adenovirus vaccine against lethal MERS-CoV challenge in a transgenic ... Morris SJ, Sebastian S, Spencer AJ, Gilbert SC (September 2016). "Simian adenoviruses as vaccine vectors". Future Virology. 11 ... The vector is a chimpanzee adenovirus modified to avoid its replication. Adenoviruses are effective vectors for inducing and ...
Kidd, AH; Garwicz D; Oberg M (1995). "Human and simian adenoviruses: phylogenetic inferences from analysis of VA RNA genes". ... These two VA RNA genes are distinct genes in the adenovirus genome. VA RNAI is the major species with VA RNAII expressed at a ... The VA (viral associated) RNA is a type of non-coding RNA found in adenovirus. It plays a role in regulating translation. There ... Ma, Y; Mathews MB (1996). "Secondary and tertiary structure in the central domain of adenovirus type 2 VA RNA I". RNA. 2 (9): ...
Yamamoto, Hiroshi; Shimojo, H (August 1971). "Multiplicity reactivation of human adenovirus type 12 and simian virus 40 ... SV40 is an abbreviation for simian vacuolating virus 40 or simian virus 40, a polyomavirus that is found in both monkeys and ... Wikimedia Commons has media related to Simian virus 40. Frequently Asked Questions about Cancer, Simian Virus 40 (SV40), and ... Barbanti-Brodano, G; Sabbioni, S; Martini, F; Negrini, M; Corallini, A; Tognon, M (2004). "Simian virus 40 infection in humans ...
Kelly, T J; Rose, J A (1971). "Simian virus 40 integration site in an adenovirus 7-simian virus 40 hybrid DNA molecule". ... adenovirus and SV40, which cause tumors in animals. He joined the faculty in the Department of Microbiology at the Johns ... "Replication of adenovirus and SV40 chromosomes in vitro". Phil. Trans. R. Soc. Lond. B. 317 (1187): 429-438. Bibcode:1987RSPTB. ... "The DNA-activated protein kinase is required for the phosphorylation of replication protein A during simian virus 40 DNA ...
2012). "Vaccine Vectors Derived from a Large Collection of Simian Adenoviruses Induce Potent Cellular Immunity Across Multiple ... 2012). "Novel Adenovirus-Based Vaccines Induce Broad and Sustained T Cell Responses to HCV in Man". Science Translational ... 2015). "Chimpanzee adenovirus- and MVA-vectored respiratory syncytial virus vaccine is safe and immunogenic in adults". Science ... 2017). "Chimpanzee Adenovirus Vector Ebola Vaccine". The New England Journal of Medicine. 376 (10): 928-938. doi:10.1056/ ...
The adenovirus E1B protein (55K) prevents p53 from regulating genes by binding to the site on p53 which binds to the genome. In ... Simian virus 40 (SV40), a polyomavirus, can cause tumors in rodent models but is not oncogenic in humans. This phenomenon has ... Adenoviruses can lead to tumors in rodent models but do not cause cancer in humans; however, they have been exploited as ... The adenovirus early region 1A (E1A) is an oncoprotein which binds to Rb and can stimulate transcription and transform cells. ...
Schuitemaker demonstrated the efficacy of the adenovirus/protein vaccines against simian immunodeficiency virus in rhesus ... "Protective efficacy of adenovirus/protein vaccines against SIV challenges in rhesus monkeys". Science. 349 (6245): 320-324. ...
A number of vectors based on other type of viruses like Adenoviruses and Papilloma virus have been used to clone genes in ... A vector based on Simian virus 40 (SV40) was used in first cloning experiment involving mammalian cells. ...
... which was originally discovered as a contaminant in a sample of simian adenovirus. Though AAV is considered to be dependent on ... Alba, R; Bosch, A; Chillon, M (2005). "Gutless adenovirus: Last-generation adenovirus for gene therapy". Gene Therapy. 12: S18- ... 1965). "Adenovirus-Associated Defective Virus Particles". Science. 149 (3685): 754-6. Bibcode:1965Sci...149..754A. doi:10.1126/ ... "Production of high-titer recombinant adeno-associated virus vectors in the absence of helper adenovirus". Journal of Virology. ...
16 October 2017). "Protective efficacy of a novel simian adenovirus vaccine against lethal MERS-CoV challenge in a transgenic ... 28 January 2015). "A Monovalent Chimpanzee Adenovirus Ebola Vaccine Boosted with MVA". The New England Journal of Medicine. 374 ... 5 February 2016). "Chimpanzee Adenovirus Vaccine Provides Multispecies Protection against Rift Valley Fever". Scientific ...
Simian foamy viruses (SFV) is an enzootic retrovirus that has high rates of cross-species transmission and has been known to ... Diseases such as HIV and human adenoviruses have been associated with NHP interactions. In places where contact between humans ... titi monkey adenovirus) is a highly divergent, sharing ...
Throughout his career, Major has conducted research on viruses including BK virus, adenoviruses, JC virus, simian virus 40 ( ... Major, E. O.; Matsumura, P. (1984). "Human embryonic kidney cells: stable transformation with an origin-defective simian virus ... Bourne, KA; Major, EO; Khoobyarian, N (1978). "A variant of adenovirus 12 producing cytoplasmic accumulation of capsid proteins ...
... starting with studying DNA replication of human adenovirus as a model. He then began to study how the genome of simian virus 40 ...
Graham, F L (1973). "A New Technique for the Assay of Infectivity of Human Adenovirus 5 DNA". Virology. 52 (2): 456−467. doi: ... McCutchan, J H (1968). "Enhancement of Infectivity of Simian Virus 40 Deoxyribonucleic Acid with Diethylaminoethyl-Dextran". J ... Nearly 70% of gene therapy clinical trials have utilized viral vectors for the gene delivery step . Adenovirus, Adeno- ... adenovirus or adeno-associated virus, and other viruses . While highly efficient for DNA delivery, notable weaknesses of viral ...
The agent was later identified as a monkey virus and named Simian Virus 40 (SV40). Sweet & Hilleman published their work in ... Hilleman at Merck observed a similar agent in the same kind of medium that they were using to develop a vaccine for adenovirus ... Maryland to attend a workshop of the National Institutes of Health entitled Simian Virus 40: A Possible Human Polyomavirus. ...
... adenoviruses, human MeSH B04.280.030.500.675 - adenoviruses, porcine MeSH B04.280.030.500.700 - adenoviruses, simian MeSH ... adenoviruses, human MeSH B04.909.204.097.500.675 - adenoviruses, porcine MeSH B04.909.204.097.500.700 - adenoviruses, simian ... simian immunodeficiency virus MeSH B04.820.650.805.851 - simian t-lymphotropic virus 1 MeSH B04.820.650.805.855 - simian t- ... simian t-lymphotropic virus 1 MeSH B04.909.777.731.805.855 - simian t-lymphotropic virus 2 MeSH B04.909.777.731.850 - ...
... a simian adenovirus used to develop ChAdOx1 Chad-e Bala or Upper Chad, a village in Iran Chad-e Pain or Lower Chad, a village ...
More specifically, the vector used is the simian group C adenovirus GRAd32, isolated from a captive gorilla, with a genome ... It is based on a novel replication defective Gorilla Adenovirus and encodes for SARS-COV-2 full length prefusion stabilized ... April 2021). "Immunogenicity of a new gorilla adenovirus vaccine candidate for COVID-19". Molecular Therapy. 29 (8): 2412-2423 ... deleted of the entire E1 and E3 regions and the native E4 region replaced with the E4 orf6 of human adenovirus 5 (hAd5). "GRAd- ...
Ginsberg showed the process by which the adenovirus caused disease after entering host cells, leading to the creation of ... At the National Institute of Allergy and Infectious Diseases, Ginsberg performed research on Simian immunodeficiency virus, a ... He was on the faculty of Western Reserve University starting in 1951, where research he conducted showed that adenoviruses, ... "he had accomplished all he could with adenoviruses" before moving on to study HIV. A resident of both Woods Hole, Massachusetts ...
1991 Chellappan, S.; Kraus, V. B.; Kroger, B.; Munger, K.; Howley, P. M.; Phelps, W. C.; Nevins, J. R. (1992). "Adenovirus E1A ... simian virus 40 tumor antigen, and human papillomavirus E7 protein share the capacity to disrupt the interaction between ...
Multiplicity reactivation has been found to occur with pathogenic viruses including influenza virus, HIV-1, adenovirus simian ...
... adenovirus simian virus 40, vaccinia virus, reovirus, poliovirus and herpes simplex virus. When HSV particles are exposed to ...
Whyte, P (1988). "Association between an oncogene and an anti-oncogene: the adenovirus E1A proteins bind to the retinoblastoma ... "Monoclonal antibodies specific for simian virus 40 tumor antigens." Journal of Virology 39.3 (1981): 861-869. Tsai, Li-Huei, et ... "Association between an oncogene and an anti-oncogene: the adenovirus E1A proteins bind to the retinoblastoma gene product." ( ... Harlow, E (1981). "Monoclonal antibodies specific for simian virus 40 tumor antigens". J Virol. 39: 861-9. PMC 171319. PMID ...
... is an adenovirus protein of 14kDa which regulates growth in all stages of the adenovirus (Ad) infection. E4orf4 partners mainly ... Toolan, H.W. and Ledinko, N. (1965) Growth and cytopathogenicity of H-viruses in human and simian cell cultures. Nature 208, 8 ... It was seen in one Phase I clinical trial that injecting mda-7 via an adenovirus directly into a tumor resulted in safe tumor ... Toolan, H.W. and Ledinko, N. (1968) Inhibition by H-l virus of the incidence of tumors produced by adenovirus 12 in hamsters. ...
... and could be preferentially transformed by adenovirus. Adenoviruses transform neuronal lineage cells much more efficiently than ... Stillman BW, Gluzman Y (August 1985). "Replication and supercoiling of simian virus 40 DNA in cell extracts from human cells". ... The cells were cultured by van der Eb; the transduction by adenovirus was performed by Frank Graham, a post-doc in van der Eb's ... However, the original adenovirus transformation was inefficient, suggesting that the cell that finally produced the HEK 293 ...
Simian mastadenovirus A Simian mastadenovirus B Simian mastadenovirus C Simian mastadenovirus D Simian mastadenovirus E Simian ... can cause potentially fatal adenovirus infections.[citation needed] Canine adenovirus 1 (CAdV-1) can lead to death in puppies, ... mastadenovirus F Simian mastadenovirus G Simian mastadenovirus H Simian mastadenovirus I Skunk mastadenovirus A Squirrel ... The name Mastadenovirus is derived from the Greek word mastos 'breast' (hence mammal) and adenovirus, named for the human ...
Many RNA viruses including SV40, adenovirus, herpes virus, Rous sarcoma virus, and influenza virus have been known to contain ... Aloni Y, Dhar R, Khoury G (October 1979). "Methylation of nuclear simian virus 40 RNAs". Journal of Virology. 32 (1): 52-60. ...
Yamamoto H, Shimojo H (August 1971). "Multiplicity reactivation of human adenovirus type 12 and simian virus 40 irradiated by ... Adenoviruses Stanford University-Adenoviruses Adenoviruses General Concepts General information on Adenovirus DNA virus ... Tupaia adenovirus (TAV) (tree shrew adenovirus 1) has been isolated from tree shrews. Otarine adenovirus 1 has been isolated ... In babies, adenoviruses can also cause coughing fits that look almost exactly like whooping cough. Adenoviruses can also cause ...
... consistent with the demonstrated role for this gene in Simian Virus 40 (SV40) viral replication. Monoallelic variants in this ... "Identification of FAM111A as an SV40 host range restriction and adenovirus helper factor". PLOS Pathog. 8 (10): e1002949. doi: ...
It was found that in the absence of the E1B-55Kd viral protein, adenovirus caused very rapid apoptosis of infected, p53(+) ... For example, the most popular retroviral vector for use in gene therapy trials has been the lentivirus Simian immunodeficiency ... Retroviruses have limited natural host cell ranges, and although adenovirus and adeno-associated virus are able to infect a ... The genetic material of the adenoviruses is not incorporated (transient) into the host cell's genetic material. The DNA ...
The foremost issue facing the recombinant adenovirus that was used is the high prevalence of the adenovirus-specific antibodies ... Malashkevich VN, Chan DC, Chutkowski CT, Kim PS (August 1998). "Crystal structure of the simian immunodeficiency virus (SIV) ... Adenoviruses are among the main causes of upper respiratory tract ailments such as the common cold. Because the vaccine ... Adenovirus vectors and many other viral vectors currently used in HIV vaccines will induce a rapid memory immune response ...
... adenovirus, HIV, HTLV, hepatitis B, hepatitis C, parvovirus B19, and influenza virus). Of possible relevance to transfusion ... "Pathogenic simian immunodeficiency virus infection is associated with expansion of the enteric virome". Cell. 151 (2): 253-66. ... adenoviruses, anelloviruses, parvoviruses, and circoviruses. Each individual had a distinct viral profile, demonstrating the ...
The most common virus used for gene delivery come from adenoviruses as they can carry up to 7.5 kb of foreign DNA and infect a ... Jackson DA, Symons RH, Berg P (October 1972). "Biochemical method for inserting new genetic information into DNA of Simian ... Viruses that have been used for gene therapy are, adenovirus, lentivirus, retrovirus and the herpes simplex virus. ... such as adenovirus, and modify its genome to have genes that code for immunogenic proteins that can spike the immune systems ...
... include the tarsiers and the simians (apes, monkeys and humans). The strepsirrhine ("bent-nosed" or "wet-nosed") primates, ... mice by helper-dependent adenovirus-based expression of gulonolactone oxidase". Human Gene Therapy. 19 (12): 1349-58. doi: ...
Family 1.A.103 The Simian Virus 5 (Parainfluenza Virus 5) SH (SV5-SH) Family 1.A.104 The Proposed Flagellar Biosynthesis Na+ ... envelope anion-channel-forming Tic110 family 1.A.19 Type A influenza virus matrix-2 channel family 1.A.20 BCL2/Adenovirus E1B- ...
... (Simian Vacuolating Virus 40 TAg) is a hexamer protein that is a dominant-acting oncoprotein derived from ... SV40 large TAg, other polyomavirus large T antigens, adenovirus E1a proteins, and oncogenic human papillomavirus E7 proteins ... Chen S, Paucha E (July 1990). "Identification of a region of simian virus 40 large T antigen required for cell transformation ...
Gendicine is a gene therapy that employs an adenovirus to deliver the tumor suppressor gene p53 to cells. It was approved in ... and 8-oxo-purines in simian kidney cells". Nucleic Acids Research. 34 (8): 2305-2315. doi:10.1093/nar/gkl099. PMC 1458282. PMID ...
Brack, Manfred (6 December 2012). Agents Transmissible from Simians to Man. Springer Science & Business Media. ISBN 978-3-642- ... adenovirus, and alphavirus infections (e.g., chikungunya, Mayaro, Ross River, Barmah Forest, O'nyong'nyong, and Sindbis viruses ...
Atkinson, R L; Dhurandhar, N V; Allison, D B; Bowen, R L; Israel, B A; Albu, J B; Augustus, A S (2004). "Human adenovirus-36 is ... JC Virus and Simian Virus 40 Infection in Humans, and Association with Human Tumors". Polyomaviruses and Human Diseases. ... Atkinson, Richard L.; Lee, Insil; Shin, Hye-Jung; He, Jia (2010). "Human adenovirus-36 antibody status is associated with ... Fusinski, Keith A (2008). Adenovirus 36 E4orf1 gene induces differentiation of 3T3-L1 cells (PhD Dissertation). Wayne State ...
Gendicine is a cancer gene therapy that delivers the tumor suppressor gene p53 using an engineered adenovirus. In 2003, it was ... In March researchers delivered a recombinant gene encoding a broadly neutralizing antibody into monkeys infected with simian ... The death of Jesse Gelsinger in a trial of an adenovirus-vectored treatment for ornithine transcarbamylase deficiency due to a ... adenoviruses, herpes simplex, vaccinia, and adeno-associated virus. Like the genetic material (DNA or RNA) in viruses, ...
Comparison of the hexon gene sequences of simian adenovirus types identified in this study with human and simian adenovirus ... at least 25 simian adenovirus types have been identified (3). Since 2011, several novel adenoviruses have been isolated from ... Hexon genes of the 10 simian adenovirus strains ranged in length from 2,739 to 2,820 nt and from 913 to 940 aa. Four strains ( ... Identification of New Provisional Simian Adenovirus Species from Captive Monkeys, China On This Page ...
Vaccine Vectors Derived from a Large Collection of Simian Adenoviruses Induce Potent Cellular Immunity Across Multiple Species ... Vaccine Vectors Derived from a Large Collection of Simian Adenoviruses Induce Potent Cellular Immunity Across Multiple Species ...
Adenoviruses, Human Adenoviruses, Simian Animals Capsid Proteins Gene Expression Regulation, Viral Haplorhini Letters To The ... Title : Identification of New Provisional Simian Adenovirus Species from Captive Monkeys, China Personal Author(s) : Foytich, ...
Adenoviruses: SV-11, SV-15, SV-17, SV-20, SV-25, SV-27, SV-36, ICH. ... H. simplex, canine herpes, simian cytomegalic, B virus Myxoviruses: Parainfluenza 1, 2, 3, SV-5; avian leukosis (RSV/RIF and ... Positive results were obtained only with sera from simian hemorrhagic fever outbreaks: Sukhumi/64, Bethesda/64, Davis/67, and ...
Comparison of the hexon gene sequences of simian adenovirus types identified in this study with human and simian adenovirus ... at least 25 simian adenovirus types have been identified (3). Since 2011, several novel adenoviruses have been isolated from ... Hexon genes of the 10 simian adenovirus strains ranged in length from 2,739 to 2,820 nt and from 913 to 940 aa. Four strains ( ... Identification of New Provisional Simian Adenovirus Species from Captive Monkeys, China On This Page ...
Simian adenoviruses do not suffer from the same disadvantages. Therefore, investigators have tested new vaccines using the ... December 2017). "Protective efficacy of a novel simian adenovirus vaccine against lethal MERS-CoV challenge in a transgenic ... Morris SJ, Sebastian S, Spencer AJ, Gilbert SC (September 2016). "Simian adenoviruses as vaccine vectors". Future Virology. 11 ... The vector is a chimpanzee adenovirus modified to avoid its replication. Adenoviruses are effective vectors for inducing and ...
Studies on the pathogenesis of Simian adenovirus-induced tumors. III. The histopathology and ultrastructure of intracranial ... SUMMARY-In newborn hamsters, the simian adenovirus 20 (SV20), when administered intracerebrally, induces undirferentiated ...
Neutralizing antibodies to human and simian adenoviruses in humans and New-World monkeysexternal icon Ersching J, Hernandez MI ...
Studies on the mechanism of enhancement of adenovirus 7 infection in African green monkey cells by simian virus 40: Formation ... Studies on the mechanism of enhancement of adenovirus 7 infection in African green monkey cells by simian virus 40: Formation ... Studies on the mechanism of enhancement of adenovirus 7 infection in African green monkey cells by simian virus 40 : Formation ... title = "Studies on the mechanism of enhancement of adenovirus 7 infection in African green monkey cells by simian virus 40: ...
So lets suppose you received a replication-defective simian adenovirus or replication-defective human adenovirus vaccine. And ... then there was a different sort of replication-defective virus that looked much better, but it was also an adenovirus. There ...
These primary cell cultures were often noted to spontaneously degenerate over time, and adenoviruses are now known to be a ... Adenovirus, a DNA virus, was first isolated in the 1950s in adenoid tissue-derived cell cultures, hence the name. ... As with polio vaccines, live adenovirus vaccines in the 1950s became contaminated with simian virus 40 (SV40), with resulting ... Adenovirus became a significant cause of economic cost and morbidity in this setting. A live oral vaccine against adenovirus ...
Immortalization of chinchilla middle ear epithelial cells by adenovirus 12-simian virus 40 hybrid virus. Jin S, Gu XX, Rhim JS ... Immortalization of human tracheal gland epithelial cells by adenovirus 12-SV40 hybrid virus. Chopra DP, Taylor GW, Mathieu PA, ... Transformation of human bronchial epithelial cells by infection with SV40 or adenovirus-12 SV40 hybrid virus, or transfection ...
Heterologous Two-Dose Vaccination with Simian Adenovirus and Poxvirus Vectors Elicits Long-Lasting Cellular Immunity to ... One such approach uses Chimpanzee Adenovirus (ChAdOx) or Modified Vaccinia Ankara (MVA) viral vectors containing the internal ...
Testing has shown that the human epithelial line is negative for simian virus 40, respiratory syncytial virus, and adenoviruses ... Transformed (Simian Virus 40) African Green Monkey Kidney Fibroblast Cells (COS-1 Line). COS-1 is a transformed cell line that ... Transformed (Simian Virus 40) African Green Monkey Kidney Fibroblast Cells (COS-7 Line). The COS-7 cell line is a line ... The cells are known to be resistant to coxsackieviruses A9 and B5, adenovirus 5, and poliovirus 2. Utilized in a wide array of ...
These primary cell cultures were often noted to spontaneously degenerate over time, and adenoviruses are now known to be a ... Adenovirus, a DNA virus, was first isolated in the 1950s in adenoid tissue-derived cell cultures, hence the name. ... As with polio vaccines, live adenovirus vaccines in the 1950s became contaminated with simian virus 40 (SV40), with resulting ... Adenovirus became a significant cause of economic cost and morbidity in this setting. A live oral vaccine against adenovirus ...
Long-term follow up of human T-cell responses to conserved HIV-1 regions elicited by DNA/simian adenovirus/MVA vaccine regimens ... simian adenoviruses isolated from chimpanzees [8,59] and gorillas [60,61], vesicular stomatitis virus (VSV) [62], adeno- ... which was engineered from a simian adenovirus Y25 isolated from a chimpanzee [67]. It is replication deficient, whereby for the ... Recombinant adenovirus type 5 HIV gag/pol/nef vaccine in South Africa: unblinded, long-term follow-up of the phase 2b HVTN 503/ ...
We report on VITT after ChAdOx1 nCoV-19, which is the only SARS-CoV-2 vaccine that includes a simian adenovirus. Disturbances ... Othman M, Labelle A, Mazzetti I, Elbatarny HS, Lillicrap D.. Adenovirus-induced thrombocytopenia: the role of von Willebrand ... of platelets have been described in association with the intravenous administration of adenovirus gene therapy vectors although ...
1. Antrobus, R.D., et al., Clinical assessment of a novel recombinant simian adenovirus ChAdOx1 as a vectored vaccine ... 2. Coughlan, L., et al., Heterologous Two-Dose Vaccination with Simian Adenovirus and Poxvirus Vectors Elicits Long-Lasting ... It uses a replication-deficient chimpanzee viral vector based on a weakened version of a common cold virus (adenovirus) that ...
Comparative analysis of simian immunodeficiency virus Gag-specific effector and memory CD8+ T cells induced by different ... Haut LH, Ratcliffe S, Pinto AR, Ertl H. Effect of preexisting immunity to adenovirus on transgene product-specific genital T ... adenovirus vectors. J Virol. 2013;87:1359-72. (FC). *21. Duraiswamy J, Freeman GJ, Coukos G. Therapeutic PD-1 pathway blockade ...
p400 is a cellular protein which binds to the adenovirus E1A oncoprotein(s). The biological function of p400 is not yet known, ... p300 Family members associate with the carboxyl terminus of simian virus 40 large tumor antigen. In: Journal of virology. 1997 ... p400 is a cellular protein which binds to the adenovirus E1A oncoprotein(s). The biological function of p400 is not yet known, ... p400 is a cellular protein which binds to the adenovirus E1A oncoprotein(s). The biological function of p400 is not yet known, ...
... a prelude to testing in a heterologous viral vector boost regimen which will include a replication-defective simian adenovirus ...
That vaccine used a simian adenovirus that was basically hollowed out and the mRNA for coding a coronavirus spike protein was ... the mRNA in the viral vector is introduced into immune cells after those immune cells take up the simian adenovirus after ...
... or simian adenovirus serotype 25, in which E1 and E3 regions are deleted with integrated expression cassette selected from SEQ ... Described is the use of an agent containing expression vector based on strain human adenovirus serotype 26 or human adenovirus ...
Vaxzevria AstraZeneca COVID-19 Vaccine (AZD1222) consists of the replication-deficient simian adenovirus vector ChAdOx1.Read ... Adenovirus Type 4 and Type 7 Vaccine elicit immunity to adenovirus serotypes 4 and 7 which are most often associated with acute ... cAd3-EBO Z EBOLA Zaire Vaccine uses a chimpanzee adenovirus (cAd3) vector to deliver Ebola genetic material.Read more... ... Jcovden COVID-19 Vaccine (Ad26.COV2.S) by Janssen, Johnson & Johnson is an adenovirus serotype 26 (Ad26) vector-based vaccine. ...
A prime-boost immunization regimen based on a simian adenovirus 36 vectored multi-stage malaria vaccine induces protective ... A real-time PCR method to rapidly titer adenovirus stocks.. Thomas, M. A., Lichtenstein, D. L., Krajcsi, P. & Wold, W. S. M., ... Application of a Fas ligand encoding a recombinant adenovirus vector for prolongation of transgene expression. Zhang, H. G., ...
Replication-defective simian (chimpanzee-derived) group C adenovirus, and modified vaccinia virus Ankara vector (MVA) ... ChAdOx1-HPV is a recombinant replication-incompetent chimpanzee-derived adenovirus (ChAdY25) viral vector. MVA-HPV is a ... Tolerability and Immunogenicity of a Chimpanzee Adenovirus (ChAdOx1)-vectored Multigenotype High Risk Human Papillomavirus ( ...
Vaxzevria AstraZeneca COVID-19 Vaccine (AZD1222) consists of the replication-deficient simian adenovirus vector ChAdOx1.Read ...
A variety of suitable promoters for prokaryotic (e.g., lac, tac, T3, T7 promoters for E. coli) and eukaryotic (e.g., simian ... adenovirus E1a and SV40 large T antigen) cells. Conditioned culture medium was collected from these transient transfectants. ... simian immunodeficiency virus (SIV) or human immunodeficiency virus (HIV); ... virus 40 early or late promoter, Rous sarcoma virus long terminal repeat promoter, cytomegalovirus promoter, adenovirus late ...
Safety and tolerability of conserved region vaccines vectored by plasmid DNA, simian adenovirus and modified vaccinia virus ... The vaccine regimen comprises adenovirus serotype 26 (Ad26) vectors encoding mosaic HIV antigens, which are designed to induced ... Pre-clinical development of a recombinant, replication-competent adenovirus serotype 4 vector vaccine expressing HIV-1 envelope ...
Simian adenovirus (organism). Code System Preferred Concept Name. Simian adenovirus (organism). Concept Status. Published. ...
  • The adenovirus expressing the antigen 85A (ChAdOx1 85A), is used as vector for a tuberculosis vaccine candidate. (wikipedia.org)
  • Adenovirus has been associated with both sporadic and epidemic disease and, with regard to infections among military recruits, who were routinely immunized against types 4 and 7 from 1971 until the cessation of vaccine production in 1996. (medscape.com)
  • A live oral vaccine against adenovirus types 4 and 7 was approved for use in this population by the US Food and Drug Administration (FDA) in 2011, and subsequent incidence of acute respiratory disease declined. (medscape.com)
  • MVA.HIVconsv will be tested as a single vaccine modality, as a prelude to testing in a heterologous viral vector boost regimen which will include a replication-defective simian adenovirus expressing the same immunogen. (clinicaltrials.gov)
  • CanSinoBio Ad5-EBOV is an adenovirus type 5 recombinant vector-based Ebola virus disease vaccine that protects against Ebola virus disease. (precisionvaccinations.com)
  • Altimmune Inc.'s AdCOVID COVID-19 vaccine candidate was based on an adenovirus-based intranasal vaccine platform and expresses the receptor-binding domain of the SARS-CoV-2 spike protein. (precisionvaccinations.com)
  • Adenovirus Type 4 and Type 7 Vaccine elicit immunity to adenovirus serotypes 4 and 7 which are most often associated with acute respiratory disease. (precisionvaccinations.com)
  • Vaxzevria AstraZeneca COVID-19 Vaccine (AZD1222) consists of the replication-deficient simian adenovirus vector ChAdOx1. (precisionvaccinations.com)
  • The vaccine regimen comprises adenovirus serotype 26 (Ad26) vectors encoding mosaic HIV antigens, which are designed to induced immune responses capable of recognizing diverse viral variants, followed by a booster with a trimeric clade C gp140 Env protein in alum adjuvant. (treatmentactiongroup.org)
  • Here we demonstrate vaccine protection against acquisition of fully heterologous, neutralization-resistant simian immunodeficiency virus (SIV) challenges in rhesus monkeys. (emmes.com)
  • To address this gap, we previously developed a simian adenovirus vectored vaccine, ChAdOx1 RVF, that encodes RVFV envelope glycoproteins. (ox.ac.uk)
  • It is a species C adenovirus - these are considered the most potent vectors for delivering vaccine and they have low seroprevalence in humans, which means that "GRAd vaccine immunogenicity is not diminished by pre-existing anti-human adenovirus antibodies," ReiThera noted. (informa.com)
  • Prevalence of serum neutralizing antibodies against chimpanzee adenovirus 63 and human adenovirus 5 in Kenyan children, in the context of vaccine vector efficacy. (ox.ac.uk)
  • Replication-defective recombinant adenovirus vectors carrying P. falciparum epitopes may be useful as part of a vaccine that raises cellular immunity to the pre-erythrocytic stage of malaria infection. (ox.ac.uk)
  • However, existing immunity to the adenovirus vector results in antibody-mediated neutralization of the vaccine vector, and reduced vaccine immunogenicity. (ox.ac.uk)
  • However, these regimes induced Compact disc4+ T-cell replies mostly, and even though T-cell replies correlated with vaccine efficiency, these approaches failed to induce protecting immunity in the majority of vaccinees, suggesting a need for more potent vectors such as adenoviruses. (rawveronica.com)
  • a immunogenic nonhuman adenovirus vaccine vector highly. (rawveronica.com)
  • Other examples of contamination include the unexpected discovery of pig virus DNA in the rotavirus vaccine GlaxoSmithKline's "Rotarix", a virus similar to simian (monkey) retrovirus in Merck's rotavirus vaccine "Rotateq" and retrovirus avian (bird) leukosis virus in a measles vaccine. (sanevax.org)
  • AZD1222 (ChAdOx1 nCoV-19), a replication-deficient simian adenovirus-vectored vaccine, has demonstrated safety, efficacy, and immunogenicity against coronavirus disease 2019 in clinical trials and real-world studies. (bvsalud.org)
  • Here we report on safety and immunogenicity of the poxvirus Modified Vaccinia Ankara (MVA) vectored vaccine expressing four Mycobacterium avium subspecies paratuberculosis antigens as a single dose or as a booster vaccine following a simian adenovirus (ChAdOx2) prime. (ox.ac.uk)
  • The ChAdOx1 nCoV-19 vaccine uses a replication-deficient simian adenovirus vector ChAdOx1 that contains the full-length structural surface glycoprotein of SARS-CoV-2 with a tissue plasminogen activator leader sequence. (drmolaplume.com)
  • The vaccine in the study - developed by AELIX Therapeutics - delivered the HIVACAT T-cell Immunogen (HTI) using a combination of DNA vector, modified vaccinia virus Ankara (MVA) vector and simian adenovirus vector ChAdOx1. (ox.ac.uk)
  • They will study an adenovirus serotype 26 vectored vaccine with a purified HIV envelope protein (the surface protein of the HIV virus), which enhances the immune responses. (harvard.edu)
  • In 2015, Barouch was lead author on a study in Science showing that a novel HIV vaccine using adenovirus serotype 26 (Ad26) regimen he and his research team developed at BIDMC provides robust protection against the virus in non-human primates. (harvard.edu)
  • The study results showed that in half of the vaccinated non-human primates the prime-boost vaccine regimen provided complete protection against a series of six repeated challenges with the simian immunodeficiency virus (SIV), a virus similar to HIV that occurs in nonhuman primates. (harvard.edu)
  • The vaccine uses a strain of common-cold virus (adenovirus serotype 26, or Ad26), engineered so that it does not cause illness, as a vector to deliver three mosaic antigens created from genes from many HIV variants. (globalhealthnewswire.com)
  • GlaxoSmithKline's (GSK) cAd3-ZEBO vaccine, involving a non-replicating chimpanzee adenovirus, has been shown to be safe in healthy volunteers and is on track for large-scale clinical trials among afflicted populations as early as January 2015. (limn.it)
  • The vaccine to be tested, dubbed Ad26.Mos4.HIV, uses an engineered adenovirus vector - a harmless relative of the common cold virus - to deliver a so-called mosaic of optimised HIV immunogens, or antigens that stimulate immune responses. (aidsmap.com)
  • Adenoviruses are effective vectors for inducing and boosting cellular immunity to encoded recombinant antigens. (wikipedia.org)
  • Of interest is the role of adenoviruses as viral vectors in vaccination and in gene therapy.For example, in viral vector vaccines against SARS-CoV2, the vector virus is used to deliver RNA encoding SARS-CoV2 spike protein into target cells. (medscape.com)
  • The cells are also often employed in transfection experiments with simian virus 40 ( SV40 ) and recombinant plasmid vectors. (microscopyu.com)
  • In addition to their role in pathology, adenoviruses are very important vectors in the gene therapy of genetic disorders and cancer [ 6 ], as they can accommodate a large DNA cargo, exhibit tropisms for multiple organs and can be engineered to decrease virulence. (biomedcentral.com)
  • Prime-boost regimes using simian adenoviral vectors and MVA expressing the clinically relevant Plasmodium falciparum ME.TRAP antigen have shown outstanding protective efficacy in mouse models. (ox.ac.uk)
  • Huang M, Gorman, CM . (1990) The simian virus 40 small t intron, present in many common expression vectors, leads to aberrant splicing. (dnabridges.com)
  • Recent advances in the development of HIV-1 vaccines using replication-incompetent adenovirus vectors. (nih.gov)
  • CMV gB vaccines using recombinant gB protein expressed from plasmid DNA and gB expressed in several different viral vectors (ALVAC, adenovirus, and vaccinia computer virus [VV]) have been investigated with animal models (9, 13, 23, 26, 31, 40, 54). (gpr139.info)
  • The latest paper by Beatrice Hahn's group about newly-discovered simian immunodeficiency viruses (SIVs) among ten troops of wild chimpanzees from Cameroon was published on line in Science express in May 2006, and in Science itself on July 28th, 2006. (aidsorigins.com)
  • In pre-clinical studies, regimens incorporating these mosaic vaccines protected monkeys against infection with an HIV-like virus called simian human immunodeficiency virus (SHIV). (globalhealthnewswire.com)
  • CCR5 signal transduction in macrophages by human immunodeficiency virus and simian immunodeficiency virus envelopes. (microbiologyresearch.org)
  • It is cross-reactive with human immunodeficiency virus type 2 and simian immunodeficiency virus (SIV). (bloodomics.org)
  • Evaluation of cytotoxic T-lymphocyte responses in human and nonhuman primate subjects infected with human immunodeficiency virus type 1 or simian/human immunodeficiency virus. (nih.gov)
  • Using computer simulation, he found genome fragments of two retrovirus variants - the HIV and the simian immunodeficiency virus (SIV) - in the reference genome of the original SARS-CoV-2 that emerged from the Huanan Seafood Market in Wuhan. (vaccineinjurynews.com)
  • Intro Passive immunization research in non-human primates and correlates of safety research in both non-human primates and human being vaccinees have proven that antibodies can donate to avoiding the acquisition of simian immunodeficiency disease (SIV) and human being immunodeficiency disease type 1 (HIV-1) (1, 10, 11C14, 25, 27, 28, 33, 35, 37, 41). (pacslab.net)
  • For this purpose, we developed a recombinant adenovirus carrying complementary DNA (cDNA) for wild type p53 (AdCMV.p53) and infected mesothelioma cell lines with this virus. (elsevier.com)
  • Eight monkeys had been immunized having a plasmid DNA create holding SIVmac239 on the plan of 0, 4, and eight weeks at a dosage of 4 mg/plasmid/inoculation, accompanied by intramuscular immunization having a recombinant adenovirus 5 (rAd5) vector holding at a dosage of 1011 contaminants at week 40. (pacslab.net)
  • One simian adenovirus species, SAdV-A, has been recognized by the International Committee on Taxonomy of Viruses ( 2 ). (cdc.gov)
  • CV-1 cells demonstrate susceptibility to a variety of viruses, including herpes simplex, Eastern and Western equine encephalitis, poliovirus 1, California encephalitis, and simian virus 40. (microscopyu.com)
  • Q6QPI9_E1B19K-01 E1B19K gene OS=[Viruses] Simian adenovirus E22 GN=E1B19K (length=573 residues). (inserm.fr)
  • In view of these findings, 50 human RBs were tested for the presence of five human DNA tumor viruses: adenovirus 12, BK virus, JC virus, and human papillomaviruses 16 and 18. (elsevier.com)
  • A0A076V686_E1B19K-01 E1B19K protein OS=[Viruses] Simian adenovirus ch1 GN=E1B19K (length=161 residues). (inserm.fr)
  • Many icosahedral viruses, such as adenovirus , use several different types of subunits to create a capsid, placing different proteins at the vertices and on the faces. (rcsb.org)
  • Broussard SR, Comuzzie AG, Leighton KL, Leland MM, Whitehead EM, Allan JS (1997) Characterization of new simian foamy viruses from African nonhuman primates. (springer.com)
  • hepatitis viruses B and C are associated with hepatocellular carcinoma (Monto and Wright 2001 ), Simian virus 40 has been linked to mesothelioma (Rizzo et al. (biomedcentral.com)
  • Therefore, investigators have tested new vaccines using the chimp adenovirus ChAdOx1 as a vector. (wikipedia.org)
  • ChAdOx1 has been derived from a chimpanzee adenovirus (ChAd) serotype Y25 engineered by λ red recombination to exchange the native E4 orf4, orf6 and orf6/7 genes for those from human adenovirus HAdV-C5. (wikipedia.org)
  • ChAdOx1-HPV is a recombinant replication-incompetent chimpanzee-derived adenovirus (ChAdY25) viral vector. (biosafety.be)
  • The vector is a chimpanzee adenovirus modified to avoid its replication. (wikipedia.org)
  • Our aim was to examine a population of children who are at risk from P. falciparum malaria for neutralizing immunity to replication-deficient recombinant chimpanzee adenovirus 63 vector (AdC63), compared to human adenovirus 5 vector (AdHu5). (ox.ac.uk)
  • Baum, SG , Wiese, WH & Reich, PR 1968, ' Studies on the mechanism of enhancement of adenovirus 7 infection in African green monkey cells by simian virus 40: Formation of adenovirus-specific RNA ', Virology , vol. 34, no. 2, pp. 373-376. (elsevier.com)
  • Adenovirus, a DNA virus, was first isolated in the 1950s in adenoid tissue-derived cell cultures, hence the name. (medscape.com)
  • An extremely hardy virus, adenovirus is ubiquitous in human and animal populations, survives long periods outside a host, and is endemic throughout the year. (medscape.com)
  • Immortalization of chinchilla middle ear epithelial cells by adenovirus 12-simian virus 40 hybrid virus. (nih.gov)
  • Immortalization of human tracheal gland epithelial cells by adenovirus 12-SV40 hybrid virus. (nih.gov)
  • The COS-1 cell line was produced via transformation of the previously established line with an origin defective mutant of simian virus 40 ( SV40 ) that codes for wild type large tumor antigen ( T antigen ). (microscopyu.com)
  • The COS-7 cell line is a line developed from the standard CV-1 African green monkey kidney line by transforming the normal cells with an origin defective mutant of simian virus 40 that codes for the wild-type large tumor antigen. (microscopyu.com)
  • Testing has established that most variants of the initial 3T3 cell line are susceptible to polyoma and simian virus 40 (SV40). (microscopyu.com)
  • Like p300, p400 is a phosphoprotein that binds to the simian virus 40 large tumor antigen (T). In anti-T coimmunoprecipitation experiments, staggered deletions spanning the amino-terminal 250 amino acids of T did not abrogate T binding to either p400 or p300. (elsevier.com)
  • Adenoviruses of the most ancient primate lineages support the theory on virus-host co-evolution. (semanticscholar.org)
  • It is reported for the first time the isolation and full-length genomic characterization of an adenovirus from the subfamily Colobinae, and it is revealed that this virus represents a novel species in the genus Mastadenovirus. (semanticscholar.org)
  • However, only in rare cases have adenoviruses been detected in cetaceans, where no clear correlation was found between presence of the virus and disease status. (biomedcentral.com)
  • Furthermore, adenovirus 12, JC virus, and simian virus 40 are able to induce RB-like tumors in rodents. (elsevier.com)
  • Mapping of the late promoter of simian virus 40. (wikidata.org)
  • Altered utilization of splice sites and 5' termini in late RNAs produced by leader region mutants of simian virus 40. (wikidata.org)
  • Relationship of replication and transcription of Simian Virus 40 DNA. (wikidata.org)
  • By inserting the triple translational enhancer sequences of human telomerase reverse transcriptase (hTERT), Simian virus 40 (SV40) and CMV downstream of the sequence of the BGH polyA, we were able to develop a novel gene expression system that significantly enhances the expression of the genes of interest. (elsevier.com)
  • For instance, simian virus 40 (PDB entry 1sva ) resembles T=7 quasisymmetry, but uses a pentamer in all of the positions. (rcsb.org)
  • The ability to immortalize human mesothelioma cells in vitro with simian virus (SV)40 and the fact that SV40 induces mesotheliomas in hamsters prompted us to look for SV40 deoxyribonucleic acid (DNA) sequences in human mesotheliomas. (elsevier.com)
  • Unlike adenovirus EIB 55K, simian computer virus 40 large T antigen, and human papillomavirus (HPV) E6 viral proteins, which all bind p53 and inhibit its function, HTLV-1 Tax does not appear to directly interact with p53 (38, 47). (careersfromscience.org)
  • Rb-deficient tumor cell lines as well as cells expressing viral oncoproteins (large tumor antigen of simian virus 40, early region 1A of adenovirus, early region 7 of papillomavirus) have low or barely detectable levels of cyclin D1. (mdc-berlin.de)
  • Brown P, Morreau-Dubois C, Gajdusek DC (1982) Persistent asymptomatic infection of the laboratory mouse by simian foamy virus type 6: a new model of retroviral latency. (springer.com)
  • Callahan ME, Switzer WM, Matthews AL, Roberts BD, Heneine W, Folks TM, Sandstrom PA (1999) Persistent zoonotic infection of a human with a simian foamy virus in the absence of an intact orf-2 accessory gene. (springer.com)
  • Development of polymerase chain reaction-based diagnostic tests for detection of Malsoor virus & adenovirus isolated from Rousettus species of bats in Maharashtra, India. (harvard.edu)
  • Using adenovirus to deliver genes to target cells is an option for researchers who are interested in a high titer virus with high transient expression levels that infect a wide range of cell types. (bloodomics.org)
  • Over the course of a year, 16 macaques received vaccines that contained mRNA encoding envelope proteins from three types of HIV found in different regions of the world along with Gag proteins from SIV, a related simian virus. (covidhealth.com)
  • The monkeys then received repeated rectal administrations of SHIV, a human-simian hybrid virus, in an effort to mimic sexual exposure. (covidhealth.com)
  • Adenovirus, herpes simplex virus (HSV), retrovirus, and lentivirus also have their advantages in transgene delivery. (biomedcentral.com)
  • In differential diagnosis, tests for other paramyxoviruses like mumps, pneumonia and simian virus type 5 must be performed due to possible cross-reactions. (meridianbioscience.com)
  • Hexon is the most abundant protein in the icosahedral capsid, and the hexon gene is commonly analyzed to characterize and determine adenovirus types ( 5 ). (cdc.gov)
  • Phylogenetic analysis of complete hexon gene nucleotide sequences from human and simian adenoviruses. (cdc.gov)
  • Comparison of the hexon gene sequences of simian adenovirus types identified in this study with human and simian adenovirus sequences from GenBank revealed 9 previously reported hypervariable regions (HVRs): HVR1-HVR9 ( 5 , 7 ). (cdc.gov)
  • For more accurate characterization and for possible species determination of adenovirus strains, it is beneficial to sequence the entire hexon gene. (cdc.gov)
  • Gene sequence-based phylogenetic analysis supports a common origin of adenoviruses that affect sea mammals. (biomedcentral.com)
  • In line with this, the identification of new animal adenoviruses, in addition to being interesting from an animal health perspective may be promising for gene therapy. (biomedcentral.com)
  • Recent data indicate that the transforming proteins of adenovirus, papillomavirus, and the polyomaviruses BK and JC all can bind to the product of the RB gene. (elsevier.com)
  • This preliminary report suggests that adenovirus-mediated p53 hyperexpression counteracts transforming properties of the large T-antigen and suggests that gene therapy may be useful in treating human malignant mesothelioma. (elsevier.com)
  • Adenovirus-mediated p53 gene transfer in advanced non-small-cell lung cancer. (vanderbilt.edu)
  • p400 is a cellular protein which binds to the adenovirus E1A oncoprotein(s). (elsevier.com)
  • GRAd-COV2 is based on what the company calls a novel and proprietary replication-defective simian adenoviral vector (GRAd) encoding the full-length coronavirus spike protein. (informa.com)
  • Adenovirus terminal protein precursor. (cshl.edu)
  • According to the ANH founder, the Chinese CanSino and Russian Sputnik V shots are two such vaccines that use the genetically engineered adenovirus type-5 vector (Ad5) to deliver the SARS-CoV-2 spike protein. (vaccineinjurynews.com)
  • Adenovirus/poxvirus and adenovirus/adenovirus-vector-based vaccines expressing SIV(SME543) Gag, Pol and Env antigens resulted in an 80% or greater reduction in the per-exposure probability of infection against repetitive, intrarectal SIV(MAC251) challenges in rhesus monkeys. (emmes.com)
  • However, the widespread seroprevalence of neutralizing antibodies to common human adenovirus serotypes limits their use. (wikipedia.org)
  • Adenovirus is often cultured from the pharynx and stool of asymptomatic children, and most adults have measurable titers of anti-adenovirus antibodies, implying prior infection. (medscape.com)
  • Recovery from adenovirus infection is associated with the development of serotype-specific neutralizing antibodies. (medscape.com)
  • It really is unclear whether that is due to cross-reactivity to ChAd63 of antibodies induced with a carefully related human being adenovirus, or by a minimal prevalence of ChAd63 attacks in human beings. (rawveronica.com)
  • Consequently, we further characterized 10 of these novel simian adenovirus strains by sequencing the entire open-reading frames of the hexon genes. (cdc.gov)
  • Hexon genes of the 10 simian adenovirus strains ranged in length from 2,739 to 2,820 nt and from 913 to 940 aa. (cdc.gov)
  • We determined the nucleotide sequences of PCR-amplified fragments from the genes of the IVa2, DNA-dependent DNA polymerase, penton base, and hexon proteins from every simian adenovirus (SAdV) serotype that originated from Old World monkeys for which the full genome sequence had not yet been published. (semanticscholar.org)
  • The partial sequences of the amplified fragments of the pol and hexon genes were closest to those of adenoviruses identified in sea lions with fatal adenoviral hepatitis, and to a Genbank-deposited sequence obtained from a harbour porpoise. (biomedcentral.com)
  • Thus, we propose a new candidate simian species, named SAdV-D, for this new cluster of macaque adenovirus strains. (cdc.gov)
  • Possessing over60 serotypes, adenovirus is recognized as the etiologic agent of various diverse syndromes. (medscape.com)
  • An evaluation of 174 whole genome sequences from HAdVs and SAdVs archived in GenBank finds that rather than separate HAdV and S adenovirus phylogenetic lineages, a single, intertwined tree is observed with all HAd ads and S AdVs forming mixed clades. (semanticscholar.org)
  • for example, human adenovirus (HAdV) C causes respiratory disease and HAdV-D provokes conjunctivitis, whereas these two pathologies can also be the result of HAdV-B infection. (biomedcentral.com)
  • Known to occur in widespread outbreaks, epidemic keratoconjunctivitis (EKC) is a severe ocular surface infection with a strong historical association with human adenovirus (HAdV). (harvard.edu)
  • The genome of adenovirus is well known and can be modified with relative ease to induce lysis or cytotoxicity of a specified cell line without affecting others. (medscape.com)
  • Genome analysis of four Old World monkey adenoviruses supports the proposed species classification of primate adenoviruses and reveals signs of possible homologous recombination. (semanticscholar.org)
  • Full genome sequence analysis of a novel adenovirus from a captive polar bear (Ursus maritimus). (semanticscholar.org)
  • Immune responses against a liver-stage malaria antigen induced by simian adenoviral vector AdCh63 and MVA prime-boost immunisation in non-human primates. (ox.ac.uk)
  • Gorman, CM , Gies D, McCray G. (1990) Transient production of proteins using adenovirus transformed cell line. (dnabridges.com)
  • Two other large trials, dubbed Mosaico and Imbokodo , are currently testing an approach that uses an adenovirus primer followed by a booster that contains a "mosaic" of proteins from multiple HIV strains. (covidhealth.com)
  • The first is lytic infection, which occurs when an adenovirus enters human epithelial cells and continues through an entire replication cycle, which results in cytolysis, cytokine production, and induction of host inflammatory response. (medscape.com)
  • Studies on the pathogenesis of Simian adenovirus-induced tumors. (core.ac.uk)
  • Our phylogenetic analysis also identified another possible new simian species: 5 strains, which were previously reported as "unassigned adenoviruses," were isolated from Old World monkeys living in captivity and in the wild ( 8 ). (cdc.gov)
  • These primary cell cultures were often noted to spontaneously degenerate over time, and adenoviruses are now known to be a common cause of asymptomatic respiratory tract infection that produces in vitro cytolysis in these tissues. (medscape.com)
  • Upon infection with adenovirus, one of three different interactions with the cells may occur. (medscape.com)
  • A species classification regarding Old World monkey adenoviruses is proposed. (semanticscholar.org)
  • RP testing and partial sequencing of 95 archived faecal samples from other squirrel monkeys housed at the KCCMR revealed the presence of three distinct, and apparently endemic species of adenoviruses, suggesting that squirrel monkeys may be the natural host of the TMAdV. (semanticscholar.org)
  • This adenovirus caused an outbreak of fatal hepatitis and enteritis in three captive sea lions of different species: California sea lion ( Zalophus californianus) , South African fur seal ( Arctocephalus pusillus) and South American sea lion ( Otaria flavescens) [ 14 ]. (biomedcentral.com)
  • The type species is Human adenovirus C (see ADENOVIRUSES, HUMAN). (harvard.edu)
  • In addition, proliferation of human mesothelioma tumours into nude mice was inhibited by in vivo adenovirus-mediated p53 transgene expression. (elsevier.com)
  • Adenoviruses commonly infect vertebrates, including humans and nonhuman primates ( 1 ). (cdc.gov)
  • 11. Haut LH, Ratcliffe S, Pinto AR, Ertl H. Effect of preexisting immunity to adenovirus on transgene product-specific genital T cell responses on vaccination of mice with a homologous vector. (southernbiotech.com)
  • Adenovirus is known to be oncogenic in rodents but not in humans. (medscape.com)
  • Investigating the occurrence and diversity of AdVs in fecal samples of apes and monkeys from different African countries and stool of humans living near gorillas in the Republic of Congo confirmed the gorilla-to-human transmission of adenovirus. (semanticscholar.org)
  • Adenoviruses are common pathogens in vertebrates, including humans. (biomedcentral.com)
  • Mechanism of host cell restriction in African green monkey kidney cells abortively infected with human adenovirus type 2. (wikidata.org)
  • In transformed cells, induction of apoptosis by adenovirus type 2 (Ad2) early region 4 ORF 4 (E4orf4) correlates with accumulation of E4orf4 in the cell membrane-cytoskeleton fraction. (rupress.org)
  • However, over a century since the first clinical descriptions of EKC, and over 60 years since the first causative agent, human adenovirus type 8, was identified, our understanding of this disorder remains limited. (harvard.edu)
  • We previously reported the detection of several new simian adenovirus strains in fecal specimens from captive monkeys in China ( 3 ). (cdc.gov)
  • Adenoviruses are immunogenic and elicit strong innate and adaptive immune responses. (medscape.com)
  • Intramuscular vaccination was more immunogenic than the intradermal route, and MVA could be used repeatedly for up to 3 times to boost adenovirus-primed responses. (ox.ac.uk)
  • Our findings suggest the need for vigilance against adenoviruses in captive and wild dolphin populations. (biomedcentral.com)