Adenoviruses, Human
Species of the genus MASTADENOVIRUS, causing a wide range of diseases in humans. Infections are mostly asymptomatic, but can be associated with diseases of the respiratory, ocular, and gastrointestinal systems. Serotypes (named with Arabic numbers) have been grouped into species designated Human adenovirus A-F.
Adenoviridae
Adenovirus Infections, Human
Adenovirus E1A Proteins
Adenovirus Early Proteins
Proteins encoded by adenoviruses that are synthesized prior to, and in the absence of, viral DNA replication. The proteins are involved in both positive and negative regulation of expression in viral and cellular genes, and also affect the stability of viral mRNA. Some are also involved in oncogenic transformation.
Adenovirus E1B Proteins
Adenovirus E3 Proteins
Adenovirus E4 Proteins
Adenovirus E1 Proteins
Genetic Vectors
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
Adenoviruses, Canine
Adenovirus E2 Proteins
Mastadenovirus
Genetic Therapy
Fowl adenovirus A
Gene Transfer Techniques
The introduction of functional (usually cloned) GENES into cells. A variety of techniques and naturally occurring processes are used for the gene transfer such as cell hybridization, LIPOSOMES or microcell-mediated gene transfer, ELECTROPORATION, chromosome-mediated gene transfer, TRANSFECTION, and GENETIC TRANSDUCTION. Gene transfer may result in genetically transformed cells and individual organisms.
HeLa Cells
Virus Replication
Receptors, Virus
Oncolytic Virotherapy
Oncolytic Viruses
Tumor-selective, replication competent VIRUSES that have antineoplastic effects. This is achieved by producing cytotoxicity-enhancing proteins and/or eliciting an antitumor immune response. They are genetically engineered so that they can replicate in CANCER cells but not in normal cells, and are used in ONCOLYTIC VIROTHERAPY.
Transduction, Genetic
Oncogene Proteins, Viral
Conjunctivitis, Viral
Base Sequence
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Cell Transformation, Viral
Transcription, Genetic
Transfection
Cytopathogenic Effect, Viral
Visible morphologic changes in cells infected with viruses. It includes shutdown of cellular RNA and protein synthesis, cell fusion, release of lysosomal enzymes, changes in cell membrane permeability, diffuse changes in intracellular structures, presence of viral inclusion bodies, and chromosomal aberrations. It excludes malignant transformation, which is CELL TRANSFORMATION, VIRAL. Viral cytopathogenic effects provide a valuable method for identifying and classifying the infecting viruses.
Helper Viruses
Defective Viruses
Viruses which lack a complete genome so that they cannot completely replicate or cannot form a protein coat. Some are host-dependent defectives, meaning they can replicate only in cell systems which provide the particular genetic function which they lack. Others, called SATELLITE VIRUSES, are able to replicate only when their genetic defect is complemented by a helper virus.
Promoter Regions, Genetic
beta-Galactosidase
Gene Expression Regulation, Viral
Gene Expression
Enterovirus
Plasmids
Cells, Cultured
Tumor Cells, Cultured
RNA, Messenger
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Atadenovirus
Simian virus 40
Amino Acid Sequence
DNA-Binding Proteins
Mice, Nude
Mutation
Cricetinae
Recombinant Fusion Proteins
Respiratory Tract Infections
Recombination, Genetic
Viruses
Cell Nucleus
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Antigens, Viral, Tumor
Transcription Factors
Serotyping
Inclusion Bodies, Viral
An area showing altered staining behavior in the nucleus or cytoplasm of a virus-infected cell. Some inclusion bodies represent "virus factories" in which viral nucleic acid or protein is being synthesized; others are merely artifacts of fixation and staining. One example, Negri bodies, are found in the cytoplasm or processes of nerve cells in animals that have died from rabies.
DNA Restriction Enzymes
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
Haplorhini
Nucleic Acid Hybridization
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
Antigens, CD46
Polymerase Chain Reaction
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Cell Line, Transformed
Dependovirus
Viral Vaccines
Gene Expression Regulation
Gastroenteritis
KB Cells
This line KB is now known to be a subline of the ubiquitous KERATIN-forming tumor cell line HeLa. It was originally thought to be derived from an epidermal carcinoma of the mouth, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via contamination by HELA CELLS. The cells are positive for keratin by immunoperoxidase staining. KB cells have been reported to contain human papillomavirus18 (HPV-18) sequences.
DNA, Recombinant
Tropism
Thymidine Kinase
Xenograft Model Antitumor Assays
Green Fluorescent Proteins
Lac Operon
Sigmodontinae
Apoptosis
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
Hybridization, Genetic
Neutralization Tests
The measurement of infection-blocking titer of ANTISERA by testing a series of dilutions for a given virus-antiserum interaction end-point, which is generally the dilution at which tissue cultures inoculated with the serum-virus mixtures demonstrate cytopathology (CPE) or the dilution at which 50% of test animals injected with serum-virus mixtures show infectivity (ID50) or die (LD50).
Vaccines, Synthetic
Cell Transformation, Neoplastic
Kidney
Protein Binding
Nuclear Proteins
Feces
NFI Transcription Factors
Neoplasms, Experimental
Culture Techniques
Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
Satellite Viruses
Defective viruses which can multiply only by association with a helper virus which complements the defective gene. Satellite viruses may be associated with certain plant viruses, animal viruses, or bacteriophages. They differ from satellite RNA; (RNA, SATELLITE) in that satellite viruses encode their own coat protein.
Microscopy, Electron
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
DNA Primers
Tumor Suppressor Protein p53
Virion
Liver
Y-Box-Binding Protein 1
Integrin alphaV
An alpha integrin with a molecular weight of 160-kDa that is found in a variety of cell types. It undergoes posttranslational cleavage into a heavy and a light chain that are connected by disulfide bonds. Integrin alphaV can combine with several different beta subunits to form heterodimers that generally bind to RGD sequence-containing extracellular matrix proteins.
Genes, Reporter
Blotting, Western
Cloning, Molecular
Luminescent Proteins
Deoxycytidine Monophosphate
Luciferases
Viral Plaque Assay
Method for measuring viral infectivity and multiplication in CULTURED CELLS. Clear lysed areas or plaques develop as the VIRAL PARTICLES are released from the infected cells during incubation. With some VIRUSES, the cells are killed by a cytopathic effect; with others, the infected cells are not killed but can be detected by their hemadsorptive ability. Sometimes the plaque cells contain VIRAL ANTIGENS which can be measured by IMMUNOFLUORESCENCE.
Binding Sites
Fluorescent Antibody Technique
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Complement Fixation Tests
Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.
Virus Attachment
Neoplasm Transplantation
Gene Deletion
Retinoblastoma Protein
Product of the retinoblastoma tumor suppressor gene. It is a nuclear phosphoprotein hypothesized to normally act as an inhibitor of cell proliferation. Rb protein is absent in retinoblastoma cell lines. It also has been shown to form complexes with the adenovirus E1A protein, the SV40 T antigen, and the human papilloma virus E7 protein.
Virology
Genes
Cell Division
DNA
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Protein Biosynthesis
AIDS Vaccines
Ganciclovir
Eye Infections, Viral
Infections of the eye caused by minute intracellular agents. These infections may lead to severe inflammation in various parts of the eye - conjunctiva, iris, eyelids, etc. Several viruses have been identified as the causative agents. Among these are Herpesvirus, Adenovirus, Poxvirus, and Myxovirus.
Cell Survival
Transcriptional Activation
Species Specificity
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Cytoplasm
Receptors, Vitronectin
Siadenovirus
Genetic Engineering
Gene Targeting
Chloramphenicol O-Acetyltransferase
An enzyme that catalyzes the acetylation of chloramphenicol to yield chloramphenicol 3-acetate. Since chloramphenicol 3-acetate does not bind to bacterial ribosomes and is not an inhibitor of peptidyltransferase, the enzyme is responsible for the naturally occurring chloramphenicol resistance in bacteria. The enzyme, for which variants are known, is found in both gram-negative and gram-positive bacteria. EC 2.3.1.28.
Centrifugation, Density Gradient
Reverse Transcriptase Polymerase Chain Reaction
Fibroblasts
Carcinoma
Immunization, Secondary
Templates, Genetic
E1A-Associated p300 Protein
Lung
Open Reading Frames
Antigens, Polyomavirus Transforming
Polyomavirus antigens which cause infection and cellular transformation. The large T antigen is necessary for the initiation of viral DNA synthesis, repression of transcription of the early region and is responsible in conjunction with the middle T antigen for the transformation of primary cells. Small T antigen is necessary for the completion of the productive infection cycle.
CHO Cells
Virus Inactivation
Flow Cytometry
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Disease Models, Animal
Trans-Activators
Cell-Free System
A fractionated cell extract that maintains a biological function. A subcellular fraction isolated by ultracentrifugation or other separation techniques must first be isolated so that a process can be studied free from all of the complex side reactions that occur in a cell. The cell-free system is therefore widely used in cell biology. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p166)
Cercopithecus aethiops
Injections, Intramuscular
Restriction Mapping
CD8-Positive T-Lymphocytes
Cytomegalovirus
A genus of the family HERPESVIRIDAE, subfamily BETAHERPESVIRINAE, infecting the salivary glands, liver, spleen, lungs, eyes, and other organs, in which they produce characteristically enlarged cells with intranuclear inclusions. Infection with Cytomegalovirus is also seen as an opportunistic infection in AIDS.
Virus Assembly
Neoplasms
Bacteriophage PRD1
Simplexvirus
Rabbits
Antibodies, Neutralizing
Enterovirus B, Human
Immunization
Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).
Peptide Biosynthesis
The production of PEPTIDES or PROTEINS by the constituents of a living organism. The biosynthesis of proteins on RIBOSOMES following an RNA template is termed translation (TRANSLATION, GENETIC). There are other, non-ribosomal peptide biosynthesis (PEPTIDE BIOSYNTHESIS, NUCLEIC ACID-INDEPENDENT) mechanisms carried out by PEPTIDE SYNTHASES and PEPTIDYLTRANSFERASES. Further modifications of peptide chains yield functional peptide and protein molecules.
Viral Core Proteins
Sequence Analysis, DNA
Virus Internalization
T-Lymphocytes, Cytotoxic
Immunized T-lymphocytes which can directly destroy appropriate target cells. These cytotoxic lymphocytes may be generated in vitro in mixed lymphocyte cultures (MLC), in vivo during a graft-versus-host (GVH) reaction, or after immunization with an allograft, tumor cell or virally transformed or chemically modified target cell. The lytic phenomenon is sometimes referred to as cell-mediated lympholysis (CML). These CD8-positive cells are distinct from NATURAL KILLER CELLS and NATURAL KILLER T-CELLS. There are two effector phenotypes: TC1 and TC2.
Enhancer Elements, Genetic
Temperature
Nasopharynx
Genes, p53
TATA Box
Immunohistochemistry
E2F Transcription Factors
A family of basic helix-loop-helix transcription factors that control expression of a variety of GENES involved in CELL CYCLE regulation. E2F transcription factors typically form heterodimeric complexes with TRANSCRIPTION FACTOR DP1 or transcription factor DP2, and they have N-terminal DNA binding and dimerization domains. E2F transcription factors can act as mediators of transcriptional repression or transcriptional activation.
Late transcription and simultaneous replication of simian adenovirus 7 DNA as revealed by spreading lytically infected cell cultures. (1/60)
Miller's technique of spreading DNA was applied to monkey cells productively infected with simian adenovirus 7. This permitted the visualization of cellular DNA transcription, both nucleolar and non-nucleolar, and of late transcription and replication of virus. Virus double-stranded DNA, thin fibres with very few nucleosome-like particles, were observed carrying either transcription or replication complexes. In addition, both RNP transcripts and replication forks were found on some virus duplex DNA. Virus single-stranded DNA replicative intermediates were identified on the basis of their increased thickness and contrast which results from the presence of a DNA binding protein. (+info)Biological activity of the intact and cleaved DNA of the simian adenovirus 7. (2/60)
Only the deproteinized DNA preparations of the simian adenovirus of the type 7 (SA 7) exhibited transforming and tumorigenic activity. The complex of the SA7 DNA with terminal protein (TP) did not exhibit either transforming or tumorigenic activity in cell cultures. In contrast to the transforming potential the infectious titers of the DNA - TP complex for the monkey kidney cells were 30-50 times higher than those of pure DNA. Cleavage of the SA7 DNA by specific endonucleases enhanced the tumorigenic potential of pure DNA, suppressed its infectivity and did not affect the lack of transformation capacity of the DNA - TP complex. The onc-gene was localized in the left terminal fragment with the minimal size 4,3x10(6)D in the case of R.Sal I. The tumorigenic activity was found to decrease with an increase in the size of the DNA fragment containing the onc-gene. (+info)Chimpanzee adenovirus CV-68 adapted as a gene delivery vector interacts with the coxsackievirus and adenovirus receptor. (3/60)
A replication-defective form of chimpanzee adenovirus type 68 (C68) has been developed to circumvent problems posed by widespread preexisting immunity to common human adenovirus vectors. To investigate the determinants of C68 tropism, its interaction with the coxsackievirus and adenovirus receptor (CAR) was studied. Although CHO cells were resistant to transduction by C68 as well as by adenovirus type 5 (Ad5), CHO cells expressing either human or murine CAR were transduced readily. C68 transduction, like Ad5 transduction, was blocked when cells were exposed to anti-CAR antibody or when virus was exposed to a soluble form of the CAR extracellular domain. These results indicate that gene delivery by C68 occurs by a CAR-dependent mechanism. (+info)Novel, chimpanzee serotype 68-based adenoviral vaccine carrier for induction of antibodies to a transgene product. (4/60)
An E1-deletion-containing adenoviral recombinant based on the chimpanzee serotype 68 (AdC68) was developed to express the rabies virus glycoprotein. Mice immunized with this construct (AdC68rab.gp) developed antibodies to rabies virus and remained resistant to challenge with an otherwise lethal dose of rabies virus. In naive mice immunized intranasally, the rabies virus-specific antibody responses elicited by AdC68rab.gp were comparable with regard to both titers and isotype profiles to those induced by an adenoviral recombinant based on human serotype 5 (Adhu5) expressing the same transgene product. In contrast, subcutaneous immunization with the AdC68rab.gp vaccine resulted in markedly lower antibody responses to the rabies virus glycoprotein than the corresponding Adhu5 vaccine. Antibodies from AdC68rab.gp-immunized mice were strongly biased towards the immunoglobulin G2a isotype. The antibody response to the rabies virus glycoprotein presented by Adhu5rab.gp was severely compromised in animals preexposed to the homologous adenovirus. In contrast, the rabies virus-specific antibody response to the AdC68rab.gp vaccine was at most marginally affected by preexisting immunity to common human adenovirus serotypes, such as 2, 4, 5, 7, and 12. This novel vaccine carrier thus offers a distinct advantage over adenoviral vaccines based on common human serotypes. (+info)Comparative sequence analysis of the largest E1A proteins of human and simian adenoviruses. (5/60)
The early region 1A (E1A) gene is the first gene expressed after infection with adenovirus and has been most extensively characterized in human adenovirus type 5 (hAd5). The E1A proteins interact with numerous cellular regulatory proteins, influencing a variety of transcriptional and cell cycle events. For this reason, these multifunctional proteins have been useful as tools for dissecting pathways regulating cell growth and gene expression. Despite the large number of studies using hAd5 E1A, relatively little is known about the function of the E1A proteins of other adenoviruses. In 1985, a comparison of E1A sequences from three human and one simian adenovirus identified three regions with higher overall levels of sequence conservation designated conserved regions (CR) 1, 2, and 3. As expected, these regions are critical for a variety of E1A functions. Since that time, the sequences of several other human and simian adenovirus E1A proteins have been determined. Using these, and two additional sequences that we determined, we report here a detailed comparison of the sequences of 15 E1A proteins representing each of the six hAd subgroups and several simian adenoviruses. These analyses refine the positioning of CR1, 2, and 3; define a fourth CR located near the carboxyl terminus of E1A; and suggest several new functions for E1A. (+info)A simian replication-defective adenoviral recombinant vaccine to HIV-1 gag. (6/60)
In animal models, E1-deleted human adenoviral recombinants of the serotype 5 (AdHu5) have shown high efficacy as vaccine carriers for different Ags including those of HIV-1. Humans are infected by common serotypes of human adenovirus such as AdHu5 early in life and a significant percentage has high levels of neutralizing Abs to these serotypes, which will very likely impair the efficacy of recombinant vaccines based on the homologous virus. To circumvent this problem, a novel replication-defective adenoviral vaccine carrier based on an E1-deleted recombinant of the chimpanzee adenovirus 68 (AdC68) was developed. An AdC68 construct expressing a codon-optimized, truncated form of gag of HIV-1 induces CD8(+) T cells to gag in mice which at the height of the immune response encompass nearly 20% of the entire splenic CD8(+) T cell population. The vaccine-induced immune response provides protection to challenge with a vaccinia gag recombinant virus. Induction of transgene-specific CD8(+) T cells and protection against viral challenge elicited by the AdC68 vaccines is not strongly inhibited in animals preimmune to AdHu5 virus. However, the response elicited by the AdHu5 vaccine is greatly attenuated in AdHu5 preimmune animals. (+info)Complete nucleotide sequences and genome organization of four chimpanzee adenoviruses. (7/60)
The complete nucleotide sequences for four adenoviruses-Simian Adenoviruses 21, 22, 23, and 24, originally isolated from chimpanzees, were determined. The genome organization of the chimpanzee adenoviruses was found to be similar to that of other adenoviruses. The viral gene products of the adenoviruses Simian Adenoviruses 22, 23, and 24 are very closely related to those of the (previously sequenced) chimpanzee adenovirus Simian Adenovirus 25. Simian Adenovirus 21 is most similar to human subgroup B adenoviruses HAdV-3, HAdV-7, and HAdV-35. Analysis of the capsid proteins hexon and fiber of the chimpanzee adenoviruses also supports the placement of Simian Adenovirus 21 in subgroup B and Simian Adenoviruses 22, 23, and 24 in subgroup E. (+info)Analysis of the first complete genome sequence of an Old World monkey adenovirus reveals a lineage distinct from the six human adenovirus species. (8/60)
Simian adenovirus 3 (SAdV-3) is one of several adenoviruses that were isolated decades ago from Old World monkeys. Determination of the complete DNA sequence of SAdV-3 permitted the first full genomic comparison of a monkey adenovirus with adenoviruses of humans (HAdVs) and chimpanzees, which are recognized formally as constituting six of the species (HAdV-A to HAdV-F) within the genus Mastadenovirus. The SAdV-3 genome is 34 246 bp in size and has a G+C content of 55.3 mol%. It contains all the genes that are characteristic of the genus Mastadenovirus and has a single VA-RNA gene and six genes in each of the E3 and E4 regions. The genetic organization is the same as that of HAdV-12, a member of the HAdV-A species. Phylogenetic analyses showed that although SAdV-3 is related marginally more closely to HAdV-A and HAdV-F than to other species, it represents a unique lineage that branched at an early stage of primate adenovirus divergence. The results imply that the genetic layout in SAdV-3 and HAdV-12 may also have characterized the common ancestor of all sequenced primate adenoviruses. (+info)
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ChAdOx1
Simian adenoviruses do not suffer from the same disadvantages. Therefore, investigators have tested new vaccines using the ... December 2017). "Protective efficacy of a novel simian adenovirus vaccine against lethal MERS-CoV challenge in a transgenic ... Morris SJ, Sebastian S, Spencer AJ, Gilbert SC (September 2016). "Simian adenoviruses as vaccine vectors". Future Virology. 11 ... The vector is a chimpanzee adenovirus modified to avoid its replication. Adenoviruses are effective vectors for inducing and ...
VA RNA
Kidd, AH; Garwicz D; Oberg M (1995). "Human and simian adenoviruses: phylogenetic inferences from analysis of VA RNA genes". ... These two VA RNA genes are distinct genes in the adenovirus genome. VA RNAI is the major species with VA RNAII expressed at a ... The VA (viral associated) RNA is a type of non-coding RNA found in adenovirus. It plays a role in regulating translation. There ... Ma, Y; Mathews MB (1996). "Secondary and tertiary structure in the central domain of adenovirus type 2 VA RNA I". RNA. 2 (9): ...
SV40
Yamamoto, Hiroshi; Shimojo, H (August 1971). "Multiplicity reactivation of human adenovirus type 12 and simian virus 40 ... SV40 is an abbreviation for simian vacuolating virus 40 or simian virus 40, a polyomavirus that is found in both monkeys and ... Wikimedia Commons has media related to Simian virus 40. Frequently Asked Questions about Cancer, Simian Virus 40 (SV40), and ... Barbanti-Brodano, G; Sabbioni, S; Martini, F; Negrini, M; Corallini, A; Tognon, M (2004). "Simian virus 40 infection in humans ...
Thomas J. Kelly (scientist)
Kelly, T J; Rose, J A (1971). "Simian virus 40 integration site in an adenovirus 7-simian virus 40 hybrid DNA molecule". ... adenovirus and SV40, which cause tumors in animals. He joined the faculty in the Department of Microbiology at the Johns ... "Replication of adenovirus and SV40 chromosomes in vitro". Phil. Trans. R. Soc. Lond. B. 317 (1187): 429-438. Bibcode:1987RSPTB. ... "The DNA-activated protein kinase is required for the phosphorylation of replication protein A during simian virus 40 DNA ...
Riccardo Cortese
2012). "Vaccine Vectors Derived from a Large Collection of Simian Adenoviruses Induce Potent Cellular Immunity Across Multiple ... 2012). "Novel Adenovirus-Based Vaccines Induce Broad and Sustained T Cell Responses to HCV in Man". Science Translational ... 2015). "Chimpanzee adenovirus- and MVA-vectored respiratory syncytial virus vaccine is safe and immunogenic in adults". Science ... 2017). "Chimpanzee Adenovirus Vector Ebola Vaccine". The New England Journal of Medicine. 376 (10): 928-938. doi:10.1056/ ...
Oncovirus
The adenovirus E1B protein (55K) prevents p53 from regulating genes by binding to the site on p53 which binds to the genome. In ... Simian virus 40 (SV40), a polyomavirus, can cause tumors in rodent models but is not oncogenic in humans. This phenomenon has ... Adenoviruses can lead to tumors in rodent models but do not cause cancer in humans; however, they have been exploited as ... The adenovirus early region 1A (E1A) is an oncoprotein which binds to Rb and can stimulate transcription and transform cells. ...
Hanneke Schuitemaker
Schuitemaker demonstrated the efficacy of the adenovirus/protein vaccines against simian immunodeficiency virus in rhesus ... "Protective efficacy of adenovirus/protein vaccines against SIV challenges in rhesus monkeys". Science. 349 (6245): 320-324. ...
Cloning vector
A number of vectors based on other type of viruses like Adenoviruses and Papilloma virus have been used to clone genes in ... A vector based on Simian virus 40 (SV40) was used in first cloning experiment involving mammalian cells. ...
Helper dependent virus
... which was originally discovered as a contaminant in a sample of simian adenovirus. Though AAV is considered to be dependent on ... Alba, R; Bosch, A; Chillon, M (2005). "Gutless adenovirus: Last-generation adenovirus for gene therapy". Gene Therapy. 12: S18- ... 1965). "Adenovirus-Associated Defective Virus Particles". Science. 149 (3685): 754-6. Bibcode:1965Sci...149..754A. doi:10.1126/ ... "Production of high-titer recombinant adeno-associated virus vectors in the absence of helper adenovirus". Journal of Virology. ...
Sarah Gilbert
16 October 2017). "Protective efficacy of a novel simian adenovirus vaccine against lethal MERS-CoV challenge in a transgenic ... 28 January 2015). "A Monovalent Chimpanzee Adenovirus Ebola Vaccine Boosted with MVA". The New England Journal of Medicine. 374 ... 5 February 2016). "Chimpanzee Adenovirus Vaccine Provides Multispecies Protection against Rift Valley Fever". Scientific ...
Cross-species transmission
Simian foamy viruses (SFV) is an enzootic retrovirus that has high rates of cross-species transmission and has been known to ... Diseases such as HIV and human adenoviruses have been associated with NHP interactions. In places where contact between humans ... titi monkey adenovirus) is a highly divergent, sharing ...
Eugene O. Major
Throughout his career, Major has conducted research on viruses including BK virus, adenoviruses, JC virus, simian virus 40 ( ... Major, E. O.; Matsumura, P. (1984). "Human embryonic kidney cells: stable transformation with an origin-defective simian virus ... Bourne, KA; Major, EO; Khoobyarian, N (1978). "A variant of adenovirus 12 producing cytoplasmic accumulation of capsid proteins ...
Bruce William Stillman
... starting with studying DNA replication of human adenovirus as a model. He then began to study how the genome of simian virus 40 ...
Intracellular delivery
Graham, F L (1973). "A New Technique for the Assay of Infectivity of Human Adenovirus 5 DNA". Virology. 52 (2): 456−467. doi: ... McCutchan, J H (1968). "Enhancement of Infectivity of Simian Virus 40 Deoxyribonucleic Acid with Diethylaminoethyl-Dextran". J ... Nearly 70% of gene therapy clinical trials have utilized viral vectors for the gene delivery step . Adenovirus, Adeno- ... adenovirus or adeno-associated virus, and other viruses . While highly efficient for DNA delivery, notable weaknesses of viral ...
Herbert Ratner
The agent was later identified as a monkey virus and named Simian Virus 40 (SV40). Sweet & Hilleman published their work in ... Hilleman at Merck observed a similar agent in the same kind of medium that they were using to develop a vaccine for adenovirus ... Maryland to attend a workshop of the National Institutes of Health entitled Simian Virus 40: A Possible Human Polyomavirus. ...
List of MeSH codes (B04)
... adenoviruses, human MeSH B04.280.030.500.675 - adenoviruses, porcine MeSH B04.280.030.500.700 - adenoviruses, simian MeSH ... adenoviruses, human MeSH B04.909.204.097.500.675 - adenoviruses, porcine MeSH B04.909.204.097.500.700 - adenoviruses, simian ... simian immunodeficiency virus MeSH B04.820.650.805.851 - simian t-lymphotropic virus 1 MeSH B04.820.650.805.855 - simian t- ... simian t-lymphotropic virus 1 MeSH B04.909.777.731.805.855 - simian t-lymphotropic virus 2 MeSH B04.909.777.731.850 - ...
Chad (disambiguation)
... a simian adenovirus used to develop ChAdOx1 Chad-e Bala or Upper Chad, a village in Iran Chad-e Pain or Lower Chad, a village ...
GRAd-COV2
More specifically, the vector used is the simian group C adenovirus GRAd32, isolated from a captive gorilla, with a genome ... It is based on a novel replication defective Gorilla Adenovirus and encodes for SARS-COV-2 full length prefusion stabilized ... April 2021). "Immunogenicity of a new gorilla adenovirus vaccine candidate for COVID-19". Molecular Therapy. 29 (8): 2412-2423 ... deleted of the entire E1 and E3 regions and the native E4 region replaced with the E4 orf6 of human adenovirus 5 (hAd5). "GRAd- ...
Harold Ginsberg
Ginsberg showed the process by which the adenovirus caused disease after entering host cells, leading to the creation of ... At the National Institute of Allergy and Infectious Diseases, Ginsberg performed research on Simian immunodeficiency virus, a ... He was on the faculty of Western Reserve University starting in 1951, where research he conducted showed that adenoviruses, ... "he had accomplished all he could with adenoviruses" before moving on to study HIV. A resident of both Woods Hole, Massachusetts ...
Peter M. Howley
1991 Chellappan, S.; Kraus, V. B.; Kroger, B.; Munger, K.; Howley, P. M.; Phelps, W. C.; Nevins, J. R. (1992). "Adenovirus E1A ... simian virus 40 tumor antigen, and human papillomavirus E7 protein share the capacity to disrupt the interaction between ...
Microbial genetics
Multiplicity reactivation has been found to occur with pathogenic viruses including influenza virus, HIV-1, adenovirus simian ...
Herpes simplex virus
... adenovirus simian virus 40, vaccinia virus, reovirus, poliovirus and herpes simplex virus. When HSV particles are exposed to ...
Ed Harlow
Whyte, P (1988). "Association between an oncogene and an anti-oncogene: the adenovirus E1A proteins bind to the retinoblastoma ... "Monoclonal antibodies specific for simian virus 40 tumor antigens." Journal of Virology 39.3 (1981): 861-869. Tsai, Li-Huei, et ... "Association between an oncogene and an anti-oncogene: the adenovirus E1A proteins bind to the retinoblastoma gene product." ( ... Harlow, E (1981). "Monoclonal antibodies specific for simian virus 40 tumor antigens". J Virol. 39: 861-9. PMC 171319. PMID ...
Anticancer gene
... is an adenovirus protein of 14kDa which regulates growth in all stages of the adenovirus (Ad) infection. E4orf4 partners mainly ... Toolan, H.W. and Ledinko, N. (1965) Growth and cytopathogenicity of H-viruses in human and simian cell cultures. Nature 208, 8 ... It was seen in one Phase I clinical trial that injecting mda-7 via an adenovirus directly into a tumor resulted in safe tumor ... Toolan, H.W. and Ledinko, N. (1968) Inhibition by H-l virus of the incidence of tumors produced by adenovirus 12 in hamsters. ...
HEK 293 cells
... and could be preferentially transformed by adenovirus. Adenoviruses transform neuronal lineage cells much more efficiently than ... Stillman BW, Gluzman Y (August 1985). "Replication and supercoiling of simian virus 40 DNA in cell extracts from human cells". ... The cells were cultured by van der Eb; the transduction by adenovirus was performed by Frank Graham, a post-doc in van der Eb's ... However, the original adenovirus transformation was inefficient, suggesting that the cell that finally produced the HEK 293 ...
Mastadenovirus
Simian mastadenovirus A Simian mastadenovirus B Simian mastadenovirus C Simian mastadenovirus D Simian mastadenovirus E Simian ... can cause potentially fatal adenovirus infections.[citation needed] Canine adenovirus 1 (CAdV-1) can lead to death in puppies, ... mastadenovirus F Simian mastadenovirus G Simian mastadenovirus H Simian mastadenovirus I Skunk mastadenovirus A Squirrel ... The name Mastadenovirus is derived from the Greek word mastos 'breast' (hence mammal) and adenovirus, named for the human ...
N6-Methyladenosine
Many RNA viruses including SV40, adenovirus, herpes virus, Rous sarcoma virus, and influenza virus have been known to contain ... Aloni Y, Dhar R, Khoury G (October 1979). "Methylation of nuclear simian virus 40 RNAs". Journal of Virology. 32 (1): 52-60. ...
Adenoviridae
Yamamoto H, Shimojo H (August 1971). "Multiplicity reactivation of human adenovirus type 12 and simian virus 40 irradiated by ... Adenoviruses Stanford University-Adenoviruses Adenoviruses General Concepts General information on Adenovirus DNA virus ... Tupaia adenovirus (TAV) (tree shrew adenovirus 1) has been isolated from tree shrews. Otarine adenovirus 1 has been isolated ... In babies, adenoviruses can also cause coughing fits that look almost exactly like whooping cough. Adenoviruses can also cause ...
Family with sequence similarity 111 member a
... consistent with the demonstrated role for this gene in Simian Virus 40 (SV40) viral replication. Monoallelic variants in this ... "Identification of FAM111A as an SV40 host range restriction and adenovirus helper factor". PLOS Pathog. 8 (10): e1002949. doi: ...
Vectors in gene therapy
It was found that in the absence of the E1B-55Kd viral protein, adenovirus caused very rapid apoptosis of infected, p53(+) ... For example, the most popular retroviral vector for use in gene therapy trials has been the lentivirus Simian immunodeficiency ... Retroviruses have limited natural host cell ranges, and although adenovirus and adeno-associated virus are able to infect a ... The genetic material of the adenoviruses is not incorporated (transient) into the host cell's genetic material. The DNA ...
HIV vaccine development
The foremost issue facing the recombinant adenovirus that was used is the high prevalence of the adenovirus-specific antibodies ... Malashkevich VN, Chan DC, Chutkowski CT, Kim PS (August 1998). "Crystal structure of the simian immunodeficiency virus (SIV) ... Adenoviruses are among the main causes of upper respiratory tract ailments such as the common cold. Because the vaccine ... Adenovirus vectors and many other viral vectors currently used in HIV vaccines will induce a rapid memory immune response ...
Human virome
... adenovirus, HIV, HTLV, hepatitis B, hepatitis C, parvovirus B19, and influenza virus). Of possible relevance to transfusion ... "Pathogenic simian immunodeficiency virus infection is associated with expansion of the enteric virome". Cell. 151 (2): 253-66. ... adenoviruses, anelloviruses, parvoviruses, and circoviruses. Each individual had a distinct viral profile, demonstrating the ...
Genetically modified virus
The most common virus used for gene delivery come from adenoviruses as they can carry up to 7.5 kb of foreign DNA and infect a ... Jackson DA, Symons RH, Berg P (October 1972). "Biochemical method for inserting new genetic information into DNA of Simian ... Viruses that have been used for gene therapy are, adenovirus, lentivirus, retrovirus and the herpes simplex virus. ... such as adenovirus, and modify its genome to have genes that code for immunogenic proteins that can spike the immune systems ...
L-gulonolactone oxidase
... include the tarsiers and the simians (apes, monkeys and humans). The strepsirrhine ("bent-nosed" or "wet-nosed") primates, ... mice by helper-dependent adenovirus-based expression of gulonolactone oxidase". Human Gene Therapy. 19 (12): 1349-58. doi: ...
Transporter Classification Database
Family 1.A.103 The Simian Virus 5 (Parainfluenza Virus 5) SH (SV5-SH) Family 1.A.104 The Proposed Flagellar Biosynthesis Na+ ... envelope anion-channel-forming Tic110 family 1.A.19 Type A influenza virus matrix-2 channel family 1.A.20 BCL2/Adenovirus E1B- ...
SV40 large T antigen
... (Simian Vacuolating Virus 40 TAg) is a hexamer protein that is a dominant-acting oncoprotein derived from ... SV40 large TAg, other polyomavirus large T antigens, adenovirus E1a proteins, and oncogenic human papillomavirus E7 proteins ... Chen S, Paucha E (July 1990). "Identification of a region of simian virus 40 large T antigen required for cell transformation ...
Head and neck cancer
Gendicine is a gene therapy that employs an adenovirus to deliver the tumor suppressor gene p53 to cells. It was approved in ... and 8-oxo-purines in simian kidney cells". Nucleic Acids Research. 34 (8): 2305-2315. doi:10.1093/nar/gkl099. PMC 1458282. PMID ...
Zika fever
Brack, Manfred (6 December 2012). Agents Transmissible from Simians to Man. Springer Science & Business Media. ISBN 978-3-642- ... adenovirus, and alphavirus infections (e.g., chikungunya, Mayaro, Ross River, Barmah Forest, O'nyong'nyong, and Sindbis viruses ...
Infections associated with diseases
Atkinson, R L; Dhurandhar, N V; Allison, D B; Bowen, R L; Israel, B A; Albu, J B; Augustus, A S (2004). "Human adenovirus-36 is ... JC Virus and Simian Virus 40 Infection in Humans, and Association with Human Tumors". Polyomaviruses and Human Diseases. ... Atkinson, Richard L.; Lee, Insil; Shin, Hye-Jung; He, Jia (2010). "Human adenovirus-36 antibody status is associated with ... Fusinski, Keith A (2008). Adenovirus 36 E4orf1 gene induces differentiation of 3T3-L1 cells (PhD Dissertation). Wayne State ...
Gene therapy
Gendicine is a cancer gene therapy that delivers the tumor suppressor gene p53 using an engineered adenovirus. In 2003, it was ... In March researchers delivered a recombinant gene encoding a broadly neutralizing antibody into monkeys infected with simian ... The death of Jesse Gelsinger in a trial of an adenovirus-vectored treatment for ornithine transcarbamylase deficiency due to a ... adenoviruses, herpes simplex, vaccinia, and adeno-associated virus. Like the genetic material (DNA or RNA) in viruses, ...
Identification of New Provisional Simian Adenovirus Species from Captive Monkeys, China - Volume 20, Number 10-October 2014 -...
Comparison of the hexon gene sequences of simian adenovirus types identified in this study with human and simian adenovirus ... at least 25 simian adenovirus types have been identified (3). Since 2011, several novel adenoviruses have been isolated from ... Hexon genes of the 10 simian adenovirus strains ranged in length from 2,739 to 2,820 nt and from 913 to 940 aa. Four strains ( ... Identification of New Provisional Simian Adenovirus Species from Captive Monkeys, China On This Page ...
Vaccine Vectors Derived from a Large Collection of Simian Adenoviruses Induce Potent Cellular Immunity Across Multiple Species ...
Identification of New Provisional Simian Adenovirus Species from Captive Monkeys, China
ArboCat Virus: Simian hemorrhagic fever (SHFV)
Adenoviruses: SV-11, SV-15, SV-17, SV-20, SV-25, SV-27, SV-36, ICH. ... H. simplex, canine herpes, simian cytomegalic, B virus Myxoviruses: Parainfluenza 1, 2, 3, SV-5; avian leukosis (RSV/RIF and ... Positive results were obtained only with sera from simian hemorrhagic fever outbreaks: Sukhumi/64, Bethesda/64, Davis/67, and ...
Identification of New Provisional Simian Adenovirus Species from Captive Monkeys, China - Volume 20, Number 10-October 2014 -...
Comparison of the hexon gene sequences of simian adenovirus types identified in this study with human and simian adenovirus ... at least 25 simian adenovirus types have been identified (3). Since 2011, several novel adenoviruses have been isolated from ... Hexon genes of the 10 simian adenovirus strains ranged in length from 2,739 to 2,820 nt and from 913 to 940 aa. Four strains ( ... Identification of New Provisional Simian Adenovirus Species from Captive Monkeys, China On This Page ...
ChAdOx1 - Wikipedia
Simian adenoviruses do not suffer from the same disadvantages. Therefore, investigators have tested new vaccines using the ... December 2017). "Protective efficacy of a novel simian adenovirus vaccine against lethal MERS-CoV challenge in a transgenic ... Morris SJ, Sebastian S, Spencer AJ, Gilbert SC (September 2016). "Simian adenoviruses as vaccine vectors". Future Virology. 11 ... The vector is a chimpanzee adenovirus modified to avoid its replication. Adenoviruses are effective vectors for inducing and ...
Studies on the pathogenesis of Simian adenovirus-induced tumors. III. The histopathology and ultrastructure of intracranial...
Science Clips - Monday, October 11, 2010
Studies on the mechanism of enhancement of adenovirus 7 infection in African green monkey cells by simian virus 40: Formation...
Studies on the mechanism of enhancement of adenovirus 7 infection in African green monkey cells by simian virus 40: Formation ... Studies on the mechanism of enhancement of adenovirus 7 infection in African green monkey cells by simian virus 40: Formation ... Studies on the mechanism of enhancement of adenovirus 7 infection in African green monkey cells by simian virus 40 : Formation ... title = "Studies on the mechanism of enhancement of adenovirus 7 infection in African green monkey cells by simian virus 40: ...
For COVID-19 Vaccines, ACIP Will Be a Critical Gatekeeper
Adenovirus: Background, Pathophysiology, Epidemiology
These primary cell cultures were often noted to spontaneously degenerate over time, and adenoviruses are now known to be a ... Adenovirus, a DNA virus, was first isolated in the 1950s in adenoid tissue-derived cell cultures, hence the name. ... As with polio vaccines, live adenovirus vaccines in the 1950s became contaminated with simian virus 40 (SV40), with resulting ... Adenovirus became a significant cause of economic cost and morbidity in this setting. A live oral vaccine against adenovirus ...
Rhim JS - Search Results - PubMed
Immortalization of chinchilla middle ear epithelial cells by adenovirus 12-simian virus 40 hybrid virus. Jin S, Gu XX, Rhim JS ... Immortalization of human tracheal gland epithelial cells by adenovirus 12-SV40 hybrid virus. Chopra DP, Taylor GW, Mathieu PA, ... Transformation of human bronchial epithelial cells by infection with SV40 or adenovirus-12 SV40 hybrid virus, or transfection ...
Vaccines | Free Full-Text | An Antigenic Thrift-Based Approach to Influenza Vaccine Design
Animal and Human Cells in Culture | Nikon's MicroscopyU
Testing has shown that the human epithelial line is negative for simian virus 40, respiratory syncytial virus, and adenoviruses ... Transformed (Simian Virus 40) African Green Monkey Kidney Fibroblast Cells (COS-1 Line). COS-1 is a transformed cell line that ... Transformed (Simian Virus 40) African Green Monkey Kidney Fibroblast Cells (COS-7 Line). The COS-7 cell line is a line ... The cells are known to be resistant to coxsackieviruses A9 and B5, adenovirus 5, and poliovirus 2. Utilized in a wide array of ...
Adenovirus: Background, Pathophysiology, Epidemiology
These primary cell cultures were often noted to spontaneously degenerate over time, and adenoviruses are now known to be a ... Adenovirus, a DNA virus, was first isolated in the 1950s in adenoid tissue-derived cell cultures, hence the name. ... As with polio vaccines, live adenovirus vaccines in the 1950s became contaminated with simian virus 40 (SV40), with resulting ... Adenovirus became a significant cause of economic cost and morbidity in this setting. A live oral vaccine against adenovirus ...
New vector and vaccine platforms: mRNA, DNA, viral vectors : Current Opinion in HIV and AIDS
Long-term follow up of human T-cell responses to conserved HIV-1 regions elicited by DNA/simian adenovirus/MVA vaccine regimens ... simian adenoviruses isolated from chimpanzees [8,59] and gorillas [60,61], vesicular stomatitis virus (VSV) [62], adeno- ... which was engineered from a simian adenovirus Y25 isolated from a chimpanzee [67]. It is replication deficient, whereby for the ... Recombinant adenovirus type 5 HIV gag/pol/nef vaccine in South Africa: unblinded, long-term follow-up of the phase 2b HVTN 503/ ...
Antibody-mediated procoagulant platelets in SARS-CoV-2-vaccination associated immune thrombotic thrombocytopenia
|...
We report on VITT after ChAdOx1 nCoV-19, which is the only SARS-CoV-2 vaccine that includes a simian adenovirus. Disturbances ... Othman M, Labelle A, Mazzetti I, Elbatarny HS, Lillicrap D.. Adenovirus-induced thrombocytopenia: the role of von Willebrand ... of platelets have been described in association with the intravenous administration of adenovirus gene therapy vectors although ...
COVID-19 vaccine AZD1222 showed robust immune responses in all participants in Phase I/II trial - AstraZeneca
1. Antrobus, R.D., et al., Clinical assessment of a novel recombinant simian adenovirus ChAdOx1 as a vectored vaccine ... 2. Coughlan, L., et al., Heterologous Two-Dose Vaccination with Simian Adenovirus and Poxvirus Vectors Elicits Long-Lasting ... It uses a replication-deficient chimpanzee viral vector based on a weakened version of a common cold virus (adenovirus) that ...
Biotin Anti-Mouse KLRG1, 2F1 | SouthernBiotech
Comparative analysis of simian immunodeficiency virus Gag-specific effector and memory CD8+ T cells induced by different ... Haut LH, Ratcliffe S, Pinto AR, Ertl H. Effect of preexisting immunity to adenovirus on transgene product-specific genital T ... adenovirus vectors. J Virol. 2013;87:1359-72. (FC). *21. Duraiswamy J, Freeman GJ, Coukos G. Therapeutic PD-1 pathway blockade ...
p300 Family members associate with the carboxyl terminus of simian virus 40 large tumor antigen<...
p400 is a cellular protein which binds to the adenovirus E1A oncoprotein(s). The biological function of p400 is not yet known, ... p300 Family members associate with the carboxyl terminus of simian virus 40 large tumor antigen. In: Journal of virology. 1997 ... p400 is a cellular protein which binds to the adenovirus E1A oncoprotein(s). The biological function of p400 is not yet known, ... p400 is a cellular protein which binds to the adenovirus E1A oncoprotein(s). The biological function of p400 is not yet known, ...
Safety and Immunogenicity of MVA.HIVconsv in HIV-1 Seropositive Adults on HAART - Full Text View - ClinicalTrials.gov
Different Types of Vaccines | History of Vaccines
Oficina Española de Patentes y Marcas - Technological information
Vaccines List | Precision Vaccinations
Vaxzevria AstraZeneca COVID-19 Vaccine (AZD1222) consists of the replication-deficient simian adenovirus vector ChAdOx1.Read ... Adenovirus Type 4 and Type 7 Vaccine elicit immunity to adenovirus serotypes 4 and 7 which are most often associated with acute ... cAd3-EBO Z EBOLA Zaire Vaccine uses a chimpanzee adenovirus (cAd3) vector to deliver Ebola genetic material.Read more... ... Jcovden COVID-19 Vaccine (Ad26.COV2.S) by Janssen, Johnson & Johnson is an adenovirus serotype 26 (Ad26) vector-based vaccine. ...
Department of Radiation Oncology - Research output
- Research Profiles at Washington University School of Medicine
A prime-boost immunization regimen based on a simian adenovirus 36 vectored multi-stage malaria vaccine induces protective ... A real-time PCR method to rapidly titer adenovirus stocks.. Thomas, M. A., Lichtenstein, D. L., Krajcsi, P. & Wold, W. S. M., ... Application of a Fas ligand encoding a recombinant adenovirus vector for prolongation of transgene expression. Zhang, H. G., ...
A first-in human, Phase 1b/2, study to evaluate the safety, tolerability and immunogenicity of HPV viral vectored vaccines in...
Replication-defective simian (chimpanzee-derived) group C adenovirus, and modified vaccinia virus Ankara vector (MVA) ... ChAdOx1-HPV is a recombinant replication-incompetent chimpanzee-derived adenovirus (ChAdY25) viral vector. MVA-HPV is a ... Tolerability and Immunogenicity of a Chimpanzee Adenovirus (ChAdOx1)-vectored Multigenotype High Risk Human Papillomavirus ( ...
Vaccines List | Precision Vaccinations
US Patent Application for ANTIBODIES WHICH BIND HUMAN CXCR3 Patent Application (Application #20110280875 issued November 17,...
A variety of suitable promoters for prokaryotic (e.g., lac, tac, T3, T7 promoters for E. coli) and eukaryotic (e.g., simian ... adenovirus E1a and SV40 large T antigen) cells. Conditioned culture medium was collected from these transient transfectants. ... simian immunodeficiency virus (SIV) or human immunodeficiency virus (HIV); ... virus 40 early or late promoter, Rous sarcoma virus long terminal repeat promoter, cytomegalovirus promoter, adenovirus late ...
The HIV Vaccines, Passive Immunization, and Antibody Gene Transfer Pipeline - Treatment Action Group
Safety and tolerability of conserved region vaccines vectored by plasmid DNA, simian adenovirus and modified vaccinia virus ... The vaccine regimen comprises adenovirus serotype 26 (Ad26) vectors encoding mosaic HIV antigens, which are designed to induced ... Pre-clinical development of a recombinant, replication-competent adenovirus serotype 4 vector vaccine expressing HIV-1 envelope ...
VaccineVectorsImmunodeficiencyRecombinant adenovirusVirusesChAdOx1Chimpanzee adenovirusVirusGeneProteinVaccinesAntibodiesGenesCandidate simianSerotypesHAdVGenomeAdenoviral vectorProteinsEpithelial cellsTumorsPhylogenetic analysisInfectionSpeciesTransgene expressionInfectImmunityHumansTypeFecalResponsesCaptive
Vaccine28
- The adenovirus expressing the antigen 85A (ChAdOx1 85A), is used as vector for a tuberculosis vaccine candidate. (wikipedia.org)
- Adenovirus has been associated with both sporadic and epidemic disease and, with regard to infections among military recruits, who were routinely immunized against types 4 and 7 from 1971 until the cessation of vaccine production in 1996. (medscape.com)
- A live oral vaccine against adenovirus types 4 and 7 was approved for use in this population by the US Food and Drug Administration (FDA) in 2011, and subsequent incidence of acute respiratory disease declined. (medscape.com)
- MVA.HIVconsv will be tested as a single vaccine modality, as a prelude to testing in a heterologous viral vector boost regimen which will include a replication-defective simian adenovirus expressing the same immunogen. (clinicaltrials.gov)
- CanSinoBio Ad5-EBOV is an adenovirus type 5 recombinant vector-based Ebola virus disease vaccine that protects against Ebola virus disease. (precisionvaccinations.com)
- Altimmune Inc.'s AdCOVID COVID-19 vaccine candidate was based on an adenovirus-based intranasal vaccine platform and expresses the receptor-binding domain of the SARS-CoV-2 spike protein. (precisionvaccinations.com)
- Adenovirus Type 4 and Type 7 Vaccine elicit immunity to adenovirus serotypes 4 and 7 which are most often associated with acute respiratory disease. (precisionvaccinations.com)
- Vaxzevria AstraZeneca COVID-19 Vaccine (AZD1222) consists of the replication-deficient simian adenovirus vector ChAdOx1. (precisionvaccinations.com)
- The vaccine regimen comprises adenovirus serotype 26 (Ad26) vectors encoding mosaic HIV antigens, which are designed to induced immune responses capable of recognizing diverse viral variants, followed by a booster with a trimeric clade C gp140 Env protein in alum adjuvant. (treatmentactiongroup.org)
- Here we demonstrate vaccine protection against acquisition of fully heterologous, neutralization-resistant simian immunodeficiency virus (SIV) challenges in rhesus monkeys. (emmes.com)
- To address this gap, we previously developed a simian adenovirus vectored vaccine, ChAdOx1 RVF, that encodes RVFV envelope glycoproteins. (ox.ac.uk)
- It is a species C adenovirus - these are considered the most potent vectors for delivering vaccine and they have low seroprevalence in humans, which means that "GRAd vaccine immunogenicity is not diminished by pre-existing anti-human adenovirus antibodies," ReiThera noted. (informa.com)
- Prevalence of serum neutralizing antibodies against chimpanzee adenovirus 63 and human adenovirus 5 in Kenyan children, in the context of vaccine vector efficacy. (ox.ac.uk)
- Replication-defective recombinant adenovirus vectors carrying P. falciparum epitopes may be useful as part of a vaccine that raises cellular immunity to the pre-erythrocytic stage of malaria infection. (ox.ac.uk)
- However, existing immunity to the adenovirus vector results in antibody-mediated neutralization of the vaccine vector, and reduced vaccine immunogenicity. (ox.ac.uk)
- However, these regimes induced Compact disc4+ T-cell replies mostly, and even though T-cell replies correlated with vaccine efficiency, these approaches failed to induce protecting immunity in the majority of vaccinees, suggesting a need for more potent vectors such as adenoviruses. (rawveronica.com)
- a immunogenic nonhuman adenovirus vaccine vector highly. (rawveronica.com)
- Other examples of contamination include the unexpected discovery of pig virus DNA in the rotavirus vaccine GlaxoSmithKline's "Rotarix", a virus similar to simian (monkey) retrovirus in Merck's rotavirus vaccine "Rotateq" and retrovirus avian (bird) leukosis virus in a measles vaccine. (sanevax.org)
- AZD1222 (ChAdOx1 nCoV-19), a replication-deficient simian adenovirus-vectored vaccine, has demonstrated safety, efficacy, and immunogenicity against coronavirus disease 2019 in clinical trials and real-world studies. (bvsalud.org)
- Here we report on safety and immunogenicity of the poxvirus Modified Vaccinia Ankara (MVA) vectored vaccine expressing four Mycobacterium avium subspecies paratuberculosis antigens as a single dose or as a booster vaccine following a simian adenovirus (ChAdOx2) prime. (ox.ac.uk)
- The ChAdOx1 nCoV-19 vaccine uses a replication-deficient simian adenovirus vector ChAdOx1 that contains the full-length structural surface glycoprotein of SARS-CoV-2 with a tissue plasminogen activator leader sequence. (drmolaplume.com)
- The vaccine in the study - developed by AELIX Therapeutics - delivered the HIVACAT T-cell Immunogen (HTI) using a combination of DNA vector, modified vaccinia virus Ankara (MVA) vector and simian adenovirus vector ChAdOx1. (ox.ac.uk)
- They will study an adenovirus serotype 26 vectored vaccine with a purified HIV envelope protein (the surface protein of the HIV virus), which enhances the immune responses. (harvard.edu)
- In 2015, Barouch was lead author on a study in Science showing that a novel HIV vaccine using adenovirus serotype 26 (Ad26) regimen he and his research team developed at BIDMC provides robust protection against the virus in non-human primates. (harvard.edu)
- The study results showed that in half of the vaccinated non-human primates the prime-boost vaccine regimen provided complete protection against a series of six repeated challenges with the simian immunodeficiency virus (SIV), a virus similar to HIV that occurs in nonhuman primates. (harvard.edu)
- The vaccine uses a strain of common-cold virus (adenovirus serotype 26, or Ad26), engineered so that it does not cause illness, as a vector to deliver three mosaic antigens created from genes from many HIV variants. (globalhealthnewswire.com)
- GlaxoSmithKline's (GSK) cAd3-ZEBO vaccine, involving a non-replicating chimpanzee adenovirus, has been shown to be safe in healthy volunteers and is on track for large-scale clinical trials among afflicted populations as early as January 2015. (limn.it)
- The vaccine to be tested, dubbed Ad26.Mos4.HIV, uses an engineered adenovirus vector - a harmless relative of the common cold virus - to deliver a so-called mosaic of optimised HIV immunogens, or antigens that stimulate immune responses. (aidsmap.com)
Vectors8
- Adenoviruses are effective vectors for inducing and boosting cellular immunity to encoded recombinant antigens. (wikipedia.org)
- Of interest is the role of adenoviruses as viral vectors in vaccination and in gene therapy.For example, in viral vector vaccines against SARS-CoV2, the vector virus is used to deliver RNA encoding SARS-CoV2 spike protein into target cells. (medscape.com)
- The cells are also often employed in transfection experiments with simian virus 40 ( SV40 ) and recombinant plasmid vectors. (microscopyu.com)
- In addition to their role in pathology, adenoviruses are very important vectors in the gene therapy of genetic disorders and cancer [ 6 ], as they can accommodate a large DNA cargo, exhibit tropisms for multiple organs and can be engineered to decrease virulence. (biomedcentral.com)
- Prime-boost regimes using simian adenoviral vectors and MVA expressing the clinically relevant Plasmodium falciparum ME.TRAP antigen have shown outstanding protective efficacy in mouse models. (ox.ac.uk)
- Huang M, Gorman, CM . (1990) The simian virus 40 small t intron, present in many common expression vectors, leads to aberrant splicing. (dnabridges.com)
- Recent advances in the development of HIV-1 vaccines using replication-incompetent adenovirus vectors. (nih.gov)
- CMV gB vaccines using recombinant gB protein expressed from plasmid DNA and gB expressed in several different viral vectors (ALVAC, adenovirus, and vaccinia computer virus [VV]) have been investigated with animal models (9, 13, 23, 26, 31, 40, 54). (gpr139.info)
Immunodeficiency7
- The latest paper by Beatrice Hahn's group about newly-discovered simian immunodeficiency viruses (SIVs) among ten troops of wild chimpanzees from Cameroon was published on line in Science express in May 2006, and in Science itself on July 28th, 2006. (aidsorigins.com)
- In pre-clinical studies, regimens incorporating these mosaic vaccines protected monkeys against infection with an HIV-like virus called simian human immunodeficiency virus (SHIV). (globalhealthnewswire.com)
- CCR5 signal transduction in macrophages by human immunodeficiency virus and simian immunodeficiency virus envelopes. (microbiologyresearch.org)
- It is cross-reactive with human immunodeficiency virus type 2 and simian immunodeficiency virus (SIV). (bloodomics.org)
- Evaluation of cytotoxic T-lymphocyte responses in human and nonhuman primate subjects infected with human immunodeficiency virus type 1 or simian/human immunodeficiency virus. (nih.gov)
- Using computer simulation, he found genome fragments of two retrovirus variants - the HIV and the simian immunodeficiency virus (SIV) - in the reference genome of the original SARS-CoV-2 that emerged from the Huanan Seafood Market in Wuhan. (vaccineinjurynews.com)
- Intro Passive immunization research in non-human primates and correlates of safety research in both non-human primates and human being vaccinees have proven that antibodies can donate to avoiding the acquisition of simian immunodeficiency disease (SIV) and human being immunodeficiency disease type 1 (HIV-1) (1, 10, 11C14, 25, 27, 28, 33, 35, 37, 41). (pacslab.net)
Recombinant adenovirus2
- For this purpose, we developed a recombinant adenovirus carrying complementary DNA (cDNA) for wild type p53 (AdCMV.p53) and infected mesothelioma cell lines with this virus. (elsevier.com)
- Eight monkeys had been immunized having a plasmid DNA create holding SIVmac239 on the plan of 0, 4, and eight weeks at a dosage of 4 mg/plasmid/inoculation, accompanied by intramuscular immunization having a recombinant adenovirus 5 (rAd5) vector holding at a dosage of 1011 contaminants at week 40. (pacslab.net)
Viruses8
- One simian adenovirus species, SAdV-A, has been recognized by the International Committee on Taxonomy of Viruses ( 2 ). (cdc.gov)
- CV-1 cells demonstrate susceptibility to a variety of viruses, including herpes simplex, Eastern and Western equine encephalitis, poliovirus 1, California encephalitis, and simian virus 40. (microscopyu.com)
- Q6QPI9_E1B19K-01 E1B19K gene OS=[Viruses] Simian adenovirus E22 GN=E1B19K (length=573 residues). (inserm.fr)
- In view of these findings, 50 human RBs were tested for the presence of five human DNA tumor viruses: adenovirus 12, BK virus, JC virus, and human papillomaviruses 16 and 18. (elsevier.com)
- A0A076V686_E1B19K-01 E1B19K protein OS=[Viruses] Simian adenovirus ch1 GN=E1B19K (length=161 residues). (inserm.fr)
- Many icosahedral viruses, such as adenovirus , use several different types of subunits to create a capsid, placing different proteins at the vertices and on the faces. (rcsb.org)
- Broussard SR, Comuzzie AG, Leighton KL, Leland MM, Whitehead EM, Allan JS (1997) Characterization of new simian foamy viruses from African nonhuman primates. (springer.com)
- hepatitis viruses B and C are associated with hepatocellular carcinoma (Monto and Wright 2001 ), Simian virus 40 has been linked to mesothelioma (Rizzo et al. (biomedcentral.com)
ChAdOx13
- Therefore, investigators have tested new vaccines using the chimp adenovirus ChAdOx1 as a vector. (wikipedia.org)
- ChAdOx1 has been derived from a chimpanzee adenovirus (ChAd) serotype Y25 engineered by λ red recombination to exchange the native E4 orf4, orf6 and orf6/7 genes for those from human adenovirus HAdV-C5. (wikipedia.org)
- ChAdOx1-HPV is a recombinant replication-incompetent chimpanzee-derived adenovirus (ChAdY25) viral vector. (biosafety.be)
Chimpanzee adenovirus2
- The vector is a chimpanzee adenovirus modified to avoid its replication. (wikipedia.org)
- Our aim was to examine a population of children who are at risk from P. falciparum malaria for neutralizing immunity to replication-deficient recombinant chimpanzee adenovirus 63 vector (AdC63), compared to human adenovirus 5 vector (AdHu5). (ox.ac.uk)
Virus29
- Baum, SG , Wiese, WH & Reich, PR 1968, ' Studies on the mechanism of enhancement of adenovirus 7 infection in African green monkey cells by simian virus 40: Formation of adenovirus-specific RNA ', Virology , vol. 34, no. 2, pp. 373-376. (elsevier.com)
- Adenovirus, a DNA virus, was first isolated in the 1950s in adenoid tissue-derived cell cultures, hence the name. (medscape.com)
- An extremely hardy virus, adenovirus is ubiquitous in human and animal populations, survives long periods outside a host, and is endemic throughout the year. (medscape.com)
- Immortalization of chinchilla middle ear epithelial cells by adenovirus 12-simian virus 40 hybrid virus. (nih.gov)
- Immortalization of human tracheal gland epithelial cells by adenovirus 12-SV40 hybrid virus. (nih.gov)
- The COS-1 cell line was produced via transformation of the previously established line with an origin defective mutant of simian virus 40 ( SV40 ) that codes for wild type large tumor antigen ( T antigen ). (microscopyu.com)
- The COS-7 cell line is a line developed from the standard CV-1 African green monkey kidney line by transforming the normal cells with an origin defective mutant of simian virus 40 that codes for the wild-type large tumor antigen. (microscopyu.com)
- Testing has established that most variants of the initial 3T3 cell line are susceptible to polyoma and simian virus 40 (SV40). (microscopyu.com)
- Like p300, p400 is a phosphoprotein that binds to the simian virus 40 large tumor antigen (T). In anti-T coimmunoprecipitation experiments, staggered deletions spanning the amino-terminal 250 amino acids of T did not abrogate T binding to either p400 or p300. (elsevier.com)
- Adenoviruses of the most ancient primate lineages support the theory on virus-host co-evolution. (semanticscholar.org)
- It is reported for the first time the isolation and full-length genomic characterization of an adenovirus from the subfamily Colobinae, and it is revealed that this virus represents a novel species in the genus Mastadenovirus. (semanticscholar.org)
- However, only in rare cases have adenoviruses been detected in cetaceans, where no clear correlation was found between presence of the virus and disease status. (biomedcentral.com)
- Furthermore, adenovirus 12, JC virus, and simian virus 40 are able to induce RB-like tumors in rodents. (elsevier.com)
- Mapping of the late promoter of simian virus 40. (wikidata.org)
- Altered utilization of splice sites and 5' termini in late RNAs produced by leader region mutants of simian virus 40. (wikidata.org)
- Relationship of replication and transcription of Simian Virus 40 DNA. (wikidata.org)
- By inserting the triple translational enhancer sequences of human telomerase reverse transcriptase (hTERT), Simian virus 40 (SV40) and CMV downstream of the sequence of the BGH polyA, we were able to develop a novel gene expression system that significantly enhances the expression of the genes of interest. (elsevier.com)
- For instance, simian virus 40 (PDB entry 1sva ) resembles T=7 quasisymmetry, but uses a pentamer in all of the positions. (rcsb.org)
- The ability to immortalize human mesothelioma cells in vitro with simian virus (SV)40 and the fact that SV40 induces mesotheliomas in hamsters prompted us to look for SV40 deoxyribonucleic acid (DNA) sequences in human mesotheliomas. (elsevier.com)
- Unlike adenovirus EIB 55K, simian computer virus 40 large T antigen, and human papillomavirus (HPV) E6 viral proteins, which all bind p53 and inhibit its function, HTLV-1 Tax does not appear to directly interact with p53 (38, 47). (careersfromscience.org)
- Rb-deficient tumor cell lines as well as cells expressing viral oncoproteins (large tumor antigen of simian virus 40, early region 1A of adenovirus, early region 7 of papillomavirus) have low or barely detectable levels of cyclin D1. (mdc-berlin.de)
- Brown P, Morreau-Dubois C, Gajdusek DC (1982) Persistent asymptomatic infection of the laboratory mouse by simian foamy virus type 6: a new model of retroviral latency. (springer.com)
- Callahan ME, Switzer WM, Matthews AL, Roberts BD, Heneine W, Folks TM, Sandstrom PA (1999) Persistent zoonotic infection of a human with a simian foamy virus in the absence of an intact orf-2 accessory gene. (springer.com)
- Development of polymerase chain reaction-based diagnostic tests for detection of Malsoor virus & adenovirus isolated from Rousettus species of bats in Maharashtra, India. (harvard.edu)
- Using adenovirus to deliver genes to target cells is an option for researchers who are interested in a high titer virus with high transient expression levels that infect a wide range of cell types. (bloodomics.org)
- Over the course of a year, 16 macaques received vaccines that contained mRNA encoding envelope proteins from three types of HIV found in different regions of the world along with Gag proteins from SIV, a related simian virus. (covidhealth.com)
- The monkeys then received repeated rectal administrations of SHIV, a human-simian hybrid virus, in an effort to mimic sexual exposure. (covidhealth.com)
- Adenovirus, herpes simplex virus (HSV), retrovirus, and lentivirus also have their advantages in transgene delivery. (biomedcentral.com)
- In differential diagnosis, tests for other paramyxoviruses like mumps, pneumonia and simian virus type 5 must be performed due to possible cross-reactions. (meridianbioscience.com)
Gene9
- Hexon is the most abundant protein in the icosahedral capsid, and the hexon gene is commonly analyzed to characterize and determine adenovirus types ( 5 ). (cdc.gov)
- Phylogenetic analysis of complete hexon gene nucleotide sequences from human and simian adenoviruses. (cdc.gov)
- Comparison of the hexon gene sequences of simian adenovirus types identified in this study with human and simian adenovirus sequences from GenBank revealed 9 previously reported hypervariable regions (HVRs): HVR1-HVR9 ( 5 , 7 ). (cdc.gov)
- For more accurate characterization and for possible species determination of adenovirus strains, it is beneficial to sequence the entire hexon gene. (cdc.gov)
- Gene sequence-based phylogenetic analysis supports a common origin of adenoviruses that affect sea mammals. (biomedcentral.com)
- In line with this, the identification of new animal adenoviruses, in addition to being interesting from an animal health perspective may be promising for gene therapy. (biomedcentral.com)
- Recent data indicate that the transforming proteins of adenovirus, papillomavirus, and the polyomaviruses BK and JC all can bind to the product of the RB gene. (elsevier.com)
- This preliminary report suggests that adenovirus-mediated p53 hyperexpression counteracts transforming properties of the large T-antigen and suggests that gene therapy may be useful in treating human malignant mesothelioma. (elsevier.com)
- Adenovirus-mediated p53 gene transfer in advanced non-small-cell lung cancer. (vanderbilt.edu)
Protein4
- p400 is a cellular protein which binds to the adenovirus E1A oncoprotein(s). (elsevier.com)
- GRAd-COV2 is based on what the company calls a novel and proprietary replication-defective simian adenoviral vector (GRAd) encoding the full-length coronavirus spike protein. (informa.com)
- Adenovirus terminal protein precursor. (cshl.edu)
- According to the ANH founder, the Chinese CanSino and Russian Sputnik V shots are two such vaccines that use the genetically engineered adenovirus type-5 vector (Ad5) to deliver the SARS-CoV-2 spike protein. (vaccineinjurynews.com)
Vaccines1
- Adenovirus/poxvirus and adenovirus/adenovirus-vector-based vaccines expressing SIV(SME543) Gag, Pol and Env antigens resulted in an 80% or greater reduction in the per-exposure probability of infection against repetitive, intrarectal SIV(MAC251) challenges in rhesus monkeys. (emmes.com)
Antibodies4
- However, the widespread seroprevalence of neutralizing antibodies to common human adenovirus serotypes limits their use. (wikipedia.org)
- Adenovirus is often cultured from the pharynx and stool of asymptomatic children, and most adults have measurable titers of anti-adenovirus antibodies, implying prior infection. (medscape.com)
- Recovery from adenovirus infection is associated with the development of serotype-specific neutralizing antibodies. (medscape.com)
- It really is unclear whether that is due to cross-reactivity to ChAd63 of antibodies induced with a carefully related human being adenovirus, or by a minimal prevalence of ChAd63 attacks in human beings. (rawveronica.com)
Genes4
- Consequently, we further characterized 10 of these novel simian adenovirus strains by sequencing the entire open-reading frames of the hexon genes. (cdc.gov)
- Hexon genes of the 10 simian adenovirus strains ranged in length from 2,739 to 2,820 nt and from 913 to 940 aa. (cdc.gov)
- We determined the nucleotide sequences of PCR-amplified fragments from the genes of the IVa2, DNA-dependent DNA polymerase, penton base, and hexon proteins from every simian adenovirus (SAdV) serotype that originated from Old World monkeys for which the full genome sequence had not yet been published. (semanticscholar.org)
- The partial sequences of the amplified fragments of the pol and hexon genes were closest to those of adenoviruses identified in sea lions with fatal adenoviral hepatitis, and to a Genbank-deposited sequence obtained from a harbour porpoise. (biomedcentral.com)
Candidate simian1
- Thus, we propose a new candidate simian species, named SAdV-D, for this new cluster of macaque adenovirus strains. (cdc.gov)
Serotypes1
- Possessing over60 serotypes, adenovirus is recognized as the etiologic agent of various diverse syndromes. (medscape.com)
HAdV3
- An evaluation of 174 whole genome sequences from HAdVs and SAdVs archived in GenBank finds that rather than separate HAdV and S adenovirus phylogenetic lineages, a single, intertwined tree is observed with all HAd ads and S AdVs forming mixed clades. (semanticscholar.org)
- for example, human adenovirus (HAdV) C causes respiratory disease and HAdV-D provokes conjunctivitis, whereas these two pathologies can also be the result of HAdV-B infection. (biomedcentral.com)
- Known to occur in widespread outbreaks, epidemic keratoconjunctivitis (EKC) is a severe ocular surface infection with a strong historical association with human adenovirus (HAdV). (harvard.edu)
Genome3
- The genome of adenovirus is well known and can be modified with relative ease to induce lysis or cytotoxicity of a specified cell line without affecting others. (medscape.com)
- Genome analysis of four Old World monkey adenoviruses supports the proposed species classification of primate adenoviruses and reveals signs of possible homologous recombination. (semanticscholar.org)
- Full genome sequence analysis of a novel adenovirus from a captive polar bear (Ursus maritimus). (semanticscholar.org)
Adenoviral vector1
- Immune responses against a liver-stage malaria antigen induced by simian adenoviral vector AdCh63 and MVA prime-boost immunisation in non-human primates. (ox.ac.uk)
Proteins2
- Gorman, CM , Gies D, McCray G. (1990) Transient production of proteins using adenovirus transformed cell line. (dnabridges.com)
- Two other large trials, dubbed Mosaico and Imbokodo , are currently testing an approach that uses an adenovirus primer followed by a booster that contains a "mosaic" of proteins from multiple HIV strains. (covidhealth.com)
Epithelial cells1
- The first is lytic infection, which occurs when an adenovirus enters human epithelial cells and continues through an entire replication cycle, which results in cytolysis, cytokine production, and induction of host inflammatory response. (medscape.com)
Tumors1
- Studies on the pathogenesis of Simian adenovirus-induced tumors. (core.ac.uk)
Phylogenetic analysis1
- Our phylogenetic analysis also identified another possible new simian species: 5 strains, which were previously reported as "unassigned adenoviruses," were isolated from Old World monkeys living in captivity and in the wild ( 8 ). (cdc.gov)
Infection2
- These primary cell cultures were often noted to spontaneously degenerate over time, and adenoviruses are now known to be a common cause of asymptomatic respiratory tract infection that produces in vitro cytolysis in these tissues. (medscape.com)
- Upon infection with adenovirus, one of three different interactions with the cells may occur. (medscape.com)
Species4
- A species classification regarding Old World monkey adenoviruses is proposed. (semanticscholar.org)
- RP testing and partial sequencing of 95 archived faecal samples from other squirrel monkeys housed at the KCCMR revealed the presence of three distinct, and apparently endemic species of adenoviruses, suggesting that squirrel monkeys may be the natural host of the TMAdV. (semanticscholar.org)
- This adenovirus caused an outbreak of fatal hepatitis and enteritis in three captive sea lions of different species: California sea lion ( Zalophus californianus) , South African fur seal ( Arctocephalus pusillus) and South American sea lion ( Otaria flavescens) [ 14 ]. (biomedcentral.com)
- The type species is Human adenovirus C (see ADENOVIRUSES, HUMAN). (harvard.edu)
Transgene expression1
- In addition, proliferation of human mesothelioma tumours into nude mice was inhibited by in vivo adenovirus-mediated p53 transgene expression. (elsevier.com)
Infect1
- Adenoviruses commonly infect vertebrates, including humans and nonhuman primates ( 1 ). (cdc.gov)
Immunity1
- 11. Haut LH, Ratcliffe S, Pinto AR, Ertl H. Effect of preexisting immunity to adenovirus on transgene product-specific genital T cell responses on vaccination of mice with a homologous vector. (southernbiotech.com)
Humans3
- Adenovirus is known to be oncogenic in rodents but not in humans. (medscape.com)
- Investigating the occurrence and diversity of AdVs in fecal samples of apes and monkeys from different African countries and stool of humans living near gorillas in the Republic of Congo confirmed the gorilla-to-human transmission of adenovirus. (semanticscholar.org)
- Adenoviruses are common pathogens in vertebrates, including humans. (biomedcentral.com)
Type3
- Mechanism of host cell restriction in African green monkey kidney cells abortively infected with human adenovirus type 2. (wikidata.org)
- In transformed cells, induction of apoptosis by adenovirus type 2 (Ad2) early region 4 ORF 4 (E4orf4) correlates with accumulation of E4orf4 in the cell membrane-cytoskeleton fraction. (rupress.org)
- However, over a century since the first clinical descriptions of EKC, and over 60 years since the first causative agent, human adenovirus type 8, was identified, our understanding of this disorder remains limited. (harvard.edu)
Fecal1
- We previously reported the detection of several new simian adenovirus strains in fecal specimens from captive monkeys in China ( 3 ). (cdc.gov)
Responses2
- Adenoviruses are immunogenic and elicit strong innate and adaptive immune responses. (medscape.com)
- Intramuscular vaccination was more immunogenic than the intradermal route, and MVA could be used repeatedly for up to 3 times to boost adenovirus-primed responses. (ox.ac.uk)
Captive1
- Our findings suggest the need for vigilance against adenoviruses in captive and wild dolphin populations. (biomedcentral.com)