Sus scrofa: A species of SWINE, in the family Suidae, comprising a number of subspecies including the domestic pig Sus scrofa domestica.Macrophages, Alveolar: Round, granular, mononuclear phagocytes found in the alveoli of the lungs. They ingest small inhaled particles resulting in degradation and presentation of the antigen to immunocompetent cells.Porcine respiratory and reproductive syndrome virus: A species of ARTERIVIRUS causing reproductive and respiratory disease in pigs. The European strain is called Lelystad virus. Airborne transmission is common.Swine: Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).Porcine Reproductive and Respiratory Syndrome: A syndrome characterized by outbreaks of late term abortions, high numbers of stillbirths and mummified or weak newborn piglets, and respiratory disease in young unweaned and weaned pigs. It is caused by PORCINE RESPIRATORY AND REPRODUCTIVE SYNDROME VIRUS. (Radostits et al., Veterinary Medicine, 8th ed, p1048)Cloning, Organism: The formation of one or more genetically identical organisms derived by vegetative reproduction from a single cell. The source nuclear material can be embryo-derived, fetus-derived, or taken from an adult somatic cell.Swine Diseases: Diseases of domestic swine and of the wild boar of the genus Sus.Nuclear Transfer Techniques: Methods of implanting a CELL NUCLEUS from a donor cell into an enucleated acceptor cell.Macrophages: The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)Cell Line: Established cell cultures that have the potential to propagate indefinitely.Fatigue: The state of weariness following a period of exertion, mental or physical, characterized by a decreased capacity for work and reduced efficiency to respond to stimuli.Databases, Protein: Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.Sequence Analysis, Protein: A process that includes the determination of AMINO ACID SEQUENCE of a protein (or peptide, oligopeptide or peptide fragment) and the information analysis of the sequence.Systems Integration: The procedures involved in combining separately developed modules, components, or subsystems so that they work together as a complete system. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Internet: A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.Proteome: The protein complement of an organism coded for by its genome.Proteins: Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.Sequence Alignment: The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.User-Computer Interface: The portion of an interactive computer program that issues messages to and receives commands from a user.Angiography, Digital Subtraction: A method of delineating blood vessels by subtracting a tissue background image from an image of tissue plus intravascular contrast material that attenuates the X-ray photons. The background image is determined from a digitized image taken a few moments before injection of the contrast material. The resulting angiogram is a high-contrast image of the vessel. This subtraction technique allows extraction of a high-intensity signal from the superimposed background information. The image is thus the result of the differential absorption of X-rays by different tissues.Adenoviruses, Human: Species of the genus MASTADENOVIRUS, causing a wide range of diseases in humans. Infections are mostly asymptomatic, but can be associated with diseases of the respiratory, ocular, and gastrointestinal systems. Serotypes (named with Arabic numbers) have been grouped into species designated Human adenovirus A-F.Peyer's Patches: Lymphoid tissue on the mucosa of the small intestine.Adenovirus Infections, Human: Respiratory and conjunctival infections caused by 33 identified serotypes of human adenoviruses.Transgenes: Genes that are introduced into an organism using GENE TRANSFER TECHNIQUES.Manufactured Materials: Substances and materials manufactured for use in various technologies and industries and for domestic use.Mice, Transgenic: Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.Genetic Vectors: DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.Adenoviridae Infections: Virus diseases caused by the ADENOVIRIDAE.Adenoviridae: A family of non-enveloped viruses infecting mammals (MASTADENOVIRUS) and birds (AVIADENOVIRUS) or both (ATADENOVIRUS). Infections may be asymptomatic or result in a variety of diseases.Ileum: The distal and narrowest portion of the SMALL INTESTINE, between the JEJUNUM and the ILEOCECAL VALVE of the LARGE INTESTINE.Internet: A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.Bibliometrics: The use of statistical methods in the analysis of a body of literature to reveal the historical development of subject fields and patterns of authorship, publication, and use. Formerly called statistical bibliography. (from The ALA Glossary of Library and Information Science, 1983)Information Storage and Retrieval: Organized activities related to the storage, location, search, and retrieval of information.Journal Impact Factor: A quantitative measure of the frequency on average with which articles in a journal have been cited in a given period of time.Search Engine: Software used to locate data or information stored in machine-readable form locally or at a distance such as an INTERNET site.PubMed: A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.MEDLINE: The premier bibliographic database of the NATIONAL LIBRARY OF MEDICINE. MEDLINE® (MEDLARS Online) is the primary subset of PUBMED and can be searched on NLM's Web site in PubMed or the NLM Gateway. MEDLINE references are indexed with MEDICAL SUBJECT HEADINGS (MeSH).User-Computer Interface: The portion of an interactive computer program that issues messages to and receives commands from a user.Databases, Bibliographic: Extensive collections, reputedly complete, of references and citations to books, articles, publications, etc., generally on a single subject or specialized subject area. Databases can operate through automated files, libraries, or computer disks. The concept should be differentiated from DATABASES, FACTUAL which is used for collections of data and facts apart from bibliographic references to them.ArchivesBiological Science Disciplines: All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.PubMed: A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.Coxsackie and Adenovirus Receptor-Like Membrane Protein: An Ig superfamily transmembrane protein that localizes to junctional complexes that occur between ENDOTHELIAL CELLS and EPTHELIAL CELLS. The protein may play a role in cell-cell adhesion and is the primary site for the attachment of ADENOVIRUSES during infection.Directories as Topic: Lists of persons or organizations, systematically arranged, usually in alphabetic or classed order, giving address, affiliations, etc., for individuals, and giving address, officers, functions, and similar data for organizations. (ALA Glossary of Library and Information Science, 1983)Publishing: "The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.Receptors, Virus: Specific molecular components of the cell capable of recognizing and interacting with a virus, and which, after binding it, are capable of generating some signal that initiates the chain of events leading to the biological response.Serial Publications: Publications in any medium issued in successive parts bearing numerical or chronological designations and intended to be continued indefinitely. (ALA Glossary of Library and Information Science, 1983, p203)Publications: Copies of a work or document distributed to the public by sale, rental, lease, or lending. (From ALA Glossary of Library and Information Science, 1983, p181)Czech Republic: Created 1 January 1993 as a result of the division of Czechoslovakia into the Czech Republic and Slovakia.Adenoviruses, Porcine: Species of the genus MASTADENOVIRUS, causing neurological disease in pigs.Hepatitis E virus: A positive-stranded RNA virus species in the genus HEPEVIRUS, causing enterically-transmitted non-A, non-B hepatitis (HEPATITIS E).Hepatitis E: Acute INFLAMMATION of the LIVER in humans; caused by HEPATITIS E VIRUS, a non-enveloped single-stranded RNA virus. Similar to HEPATITIS A, its incubation period is 15-60 days and is enterically transmitted, usually by fecal-oral transmission.Spain: Parliamentary democracy located between France on the northeast and Portugual on the west and bordered by the Atlantic Ocean and the Mediterranean Sea.Abattoirs: Places where animals are slaughtered and dressed for market.Meat: The edible portions of any animal used for food including domestic mammals (the major ones being cattle, swine, and sheep) along with poultry, fish, shellfish, and game.Swine: Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).Mastadenovirus: A genus of ADENOVIRIDAE that infects MAMMALS including humans and causes a wide range of diseases. The type species is Human adenovirus C (see ADENOVIRUSES, HUMAN).ItalyClassical swine fever virus: A species of the PESTIVIRUS genus causing exceedingly contagious and fatal hemorrhagic disease of swine.Classical Swine Fever: An acute, highly contagious disease affecting swine of all ages and caused by the CLASSICAL SWINE FEVER VIRUS. It has a sudden onset with high morbidity and mortality.African Swine Fever Virus: The lone species of the genus Asfivirus. It infects domestic and wild pigs, warthogs, and bushpigs. Disease is endemic in domestic swine in many African countries and Sardinia. Soft ticks of the genus Ornithodoros are also infected and act as vectors.Swine: Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).African Swine Fever: A sometimes fatal ASFIVIRUS infection of pigs, characterized by fever, cough, diarrhea, hemorrhagic lymph nodes, and edema of the gallbladder. It is transmitted between domestic swine by direct contact, ingestion of infected meat, or fomites, or mechanically by biting flies or soft ticks (genus Ornithodoros).Pestivirus: A genus of FLAVIVIRIDAE, also known as mucosal disease virus group, which is not arthropod-borne. Transmission is by direct and indirect contact, and by transplacental and congenital transmission. Species include BORDER DISEASE VIRUS, bovine viral diarrhea virus (DIARRHEA VIRUS, BOVINE VIRAL), and CLASSICAL SWINE FEVER VIRUS.Border disease virus: A species of PESTIVIRUS causing a congenital sheep disease characterized by an abnormally hairy birth-coat, tremors, and poor growth.Iridoviridae: A family of large icosahedral DNA viruses infecting insects and poikilothermic vertebrates. Genera include IRIDOVIRUS; RANAVIRUS; Chloriridovirus; Megalocytivirus; and Lymphocystivirus.Pestivirus Infections: Infections with viruses of the genus PESTIVIRUS, family FLAVIVIRIDAE.Yellow fever virus: The type species of the FLAVIVIRUS genus. Principal vector transmission to humans is by AEDES spp. mosquitoes.Dictionaries, MedicalDictionaries as Topic: Lists of words, usually in alphabetical order, giving information about form, pronunciation, etymology, grammar, and meaning.Dictionaries, ChemicalTerminology as Topic: The terms, expressions, designations, or symbols used in a particular science, discipline, or specialized subject area.RNA Viruses: Viruses whose genetic material is RNA.Peer Group: Group composed of associates of same species, approximately the same age, and usually of similar rank or social status.Video Games: A form of interactive entertainment in which the player controls electronically generated images that appear on a video display screen. This includes video games played in the home on special machines or home computers, and those played in arcades.Videotape Recording: Recording of visual and sometimes sound signals on magnetic tape.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.Journal Impact Factor: A quantitative measure of the frequency on average with which articles in a journal have been cited in a given period of time.Peer Review, Research: The evaluation by experts of the quality and pertinence of research or research proposals of other experts in the same field. Peer review is used by editors in deciding which submissions warrant publication, by granting agencies to determine which proposals should be funded, and by academic institutions in tenure decisions.Peer Review: An organized procedure carried out by a select committee of professionals in evaluating the performance of other professionals in meeting the standards of their specialty. Review by peers is used by editors in the evaluation of articles and other papers submitted for publication. Peer review is used also in the evaluation of grant applications. It is applied also in evaluating the quality of health care provided to patients.Television: The transmission and reproduction of transient images of fixed or moving objects. An electronic system of transmitting such images together with sound over a wire or through space by apparatus that converts light and sound into electrical waves and reconverts them into visible light rays and audible sound. (From Webster, 3rd ed)Publishing: "The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.Bibliometrics: The use of statistical methods in the analysis of a body of literature to reveal the historical development of subject fields and patterns of authorship, publication, and use. Formerly called statistical bibliography. (from The ALA Glossary of Library and Information Science, 1983)
(1/13) A recombinant E1-deleted porcine adenovirus-3 as an expression vector.

Replication-defective E1-deleted porcine adenoviruses (PAVs) are attractive vectors for vaccination. As a prerequisite for generating PAV-3 vectors containing complete deletion of E1, we transfected VIDO R1 cells (fetal porcine retina cells transformed with E1 region of human adenovirus 5) with a construct containing PAV-3 E1B(large) coding sequences under the control of HCMV promoter. A cell line named VR1BL could be isolated that expressed E1B(large) of PAV-3 and also complemented PAV214 (E1A+E1B(small) deleted). The VR1BL cells could be efficiently transfected with DNA and allowed the rescue and propagation of recombinant PAV507 containing a triple stop codon inserted in the E1B(large) coding sequence. In addition, recombinant PAV227 containing complete deletion of E1 (E1A+E1B(small) + E1B(large)) could be successfully rescued using VR1BL cell line. Recombinant PAV227 replicated as efficiently as wild-type in VR1BL cells but not in VIDO R1 cells, suggesting that E1B(large) was essential for replication of PAV-3. Next, we constructed recombinant PAV219 by inserting green fluorescent (GFP) protein gene flanked by a promoter and a poly(A) in the E1 region of the PAV227 genome. We demonstrated that PAV219 was able to transduce and direct expression of GFP in some human cell lines.  (+info)

(2/13) Characterization of cis-acting sequences involved in packaging porcine adenovirus type 3.

Encapsidation of adenovirus DNA involves specific interactions between cis-acting genomic DNA sequences and trans-acting proteins. The cis-acting packaging domain located near the left inverted terminal repeat is composed of a series of redundant but not functionally equivalent motifs. Such motifs are made up of the consensus sequence 5'-TTTGN(8)CG-3' and 5'-TTTG/A-3' in human adenovirus 5 (HAV-5) and canine adenovirus-2 (CAV-2), respectively. To gain comparative insight into adenovirus encapsidation, we examined the packaging domain of porcine adenovirus-3 (PAV-3). Using deletion mutants, we localized the PAV-3 packaging domain to 319 bp (nt 212 to 531), which contains six cis-acting elements. However, this domain does not contain the consensus motifs identified in HAV-5. In addition, consensus motif found in CAV-2 is present only once in PAV-3. Instead, PAV-3 packaging domain appears to contain AT/GC-rich sequences. The packaging motifs of PAV-3, which are functionally redundant but not equivalent, are located at the left end of the genome.  (+info)

(3/13) Porcine adenovirus type 3 E1 transcriptional control region contains a bifunctional regulatory element.

We identified a bifunctional regulatory element located between nt 374 and 431 upstream of TATA box of porcine adenovirus (PAV) 3 E1A promoter. Deletion of the element dramatically reduced the steady-state level of E1A mRNA, but increased that of E1B, which lies immediately downstream of E1A. The mutant virus displayed defective replication at early times of infection, but replicated nearly as efficiently as wild-type PAV-3 at late times of infection. This defect was complemented with coinfecting wild-type virus in a mixed infection. The results indicated that the upstream activation sequences (UAS) of E1A overlap the upstream repression sequences (URS) of E1B, although both transcription units are transcribed from different promoters.  (+info)

(4/13) Detection of bovine and porcine adenoviruses for tracing the source of fecal contamination.

In this study, a molecular procedure for the detection of adenoviruses of animal origin was developed to evaluate the level of excretion of these viruses by swine and cattle and to design a test to facilitate the tracing of specific sources of environmental viral contamination. Two sets of oligonucleotides were designed, one to detect porcine adenoviruses and the other to detect bovine and ovine adenoviruses. The specificity of the assays was assessed in 31 fecal samples and 12 sewage samples that were collected monthly during a 1-year period. The data also provided information on the environmental prevalence of animal adenoviruses. Porcine adenoviruses were detected in 17 of 24 (70%) pools of swine samples studied, with most isolates being closely related to serotype 3. Bovine adenoviruses were present in 6 of 8 (75%) pools studied, with strains belonging to the genera Mastadenovirus and Atadenovirus and being similar to bovine adenoviruses of types 2, 4, and 7. These sets of primers produced negative results in nested PCR assays when human adenovirus controls and urban-sewage samples were tested. Likewise, the sets of primers previously designed for detection of human adenovirus also produced negative results with animal adenoviruses. These results indicate the importance of further studies to evaluate the usefulness of these tests to trace the source of fecal contamination in water and food and for environmental studies.  (+info)

(5/13) Viral RNAs detected in virions of porcine adenovirus type 3.

It has been demonstrated that cellular and viral RNAs were packaged in the virions of human cytomegalovirus (CMV) and herpes simplex virus 1 (HSV 1), members of the Herpesviridae family, both of which are enveloped double-stranded DNA viruses. Here, we provide evidence suggesting that RNAs are packaged in the virions of porcine adenovirus type 3 (PAdV-3), which is a member of the Adenoviridae family, a non-enveloped double-stranded DNA virus. The RNAs packaged in PAdV-3 virions were enriched in the size range of 300-1000 bases long. By reverse transcription (RT) of RNAs isolated from purified PAdV-3 virions, PCR amplification, and DNA sequence analysis of PCR products, we determined the identities of some viral RNAs contained in PAdV-3 virions. The results indicated that the RNAs representing transcripts from E1A, E1B, DNA binding protein (DBP), DNA polymerase (POL), E4 and some of the late genes including pIIIA, pIII, pV, Hexon, 33 K, and fiber were detected from purified PAdV-3 virions. In contrast, we could not detect the RNAs representing transcripts of precursor terminal protein (pTP), 52 kDa, pX, or 100-kDa protein genes in purified virions. Because the transcripts of pIX, IVa2, E3, protease, pVI, pVII, and pVIII overlap with those of other genes in PAdV-3, we could not definitely conclude that RNAs representing these transcripts were packaged in virions although the expected DNA fragments were produced by RT-PCR in the RNAs isolated from purified virions.  (+info)

(6/13) Porcine adenovirus serotype 3 internalization is independent of CAR and alphavbeta3 or alphavbeta5 integrin.

Nonhuman adenoviruses including porcine adenovirus serotype 3 (PAd3) are emerging vectors for gene delivery. PAd3 efficiently transduces human and murine cells in culture, and circumvents preexisting humoral immunity in humans. The coxsackievirus-adenovirus receptor (CAR) serves as a primary receptor and alphavbeta3 or alphavbeta5 integrin as a secondary receptor for several human adenovirus (HAd) subtypes including HAd5. In this study, we deduced the role of CAR, alphavbeta3 or alphavbeta5 integrin in PAd3 internalization. Transduction experiments were conducted in human mammary epithelial (MCF-10A) cells using replication-defective PAd-GFP (PAd3 vector expressing green fluorescent protein [GFP]) and HAd-GFP (HAd5 vector expressing GFP). MCF-10A cells were treated with or without anti-human CAR, or anti-alphavbeta3 or anti-alphavbeta5 integrin antibodies prior to infection with HAd-GFP or PAd-GFP. Significant (P <0.05) inhibition in transduction by HAd-GFP was observed in antibody-treated cells as compared to untreated cells, whereas transduction by PAd-GFP remained to similar levels irrespective of the treatment. To study the adenoviral fiber knob-mediated virus interference, MCF-10A cells were treated with or without the recombinant HAd5 or PAd3 knob followed by infection with HAd-GFP or PAd-GFP. Significant (P <0.05) inhibition was observed only in transduction of the homologous vector. These results suggested that PAd3 internalization was CAR- as well as alphavbeta3 or alphavbeta5 integrin-independent and the primary receptor for HAd5 and PAd3 were distinct. CAR- and alphavbeta3 or alphavbeta5 integrin-independent entry of PAd3 vectors may have implications in targeting cell types that are not efficiently transduced by other adenoviral vectors.  (+info)

(7/13) A newly identified interaction between IVa2 and pVIII proteins during porcine adenovirus type 3 infection.

The adenovirus IVa2 is an intermediate viral gene product that appears to perform multiple essential roles in viral infection. Using IVa2 as bait in the yeast two-hybrid system, we screened selected open reading frames (ORFs) of porcine adenovirus (PAdV)-3 for potential interaction with IVa2. Interestingly, pVIII showed specific interaction with IVa2. The yeast two-hybrid findings were validated by GST pull-down assays, in vitro binding studies employing cell-free coupled transcription-translation products and in vitro co-immunoprecipitations using protein-specific antibodies. Finally, we demonstrated that IVa2 specifically interacts with pVIII during PAdV-3 infection.  (+info)

(8/13) Porcine adenovirus type 3 E1B large protein downregulates the induction of IL-8.

Replication-defective (E1-E3 deleted) adenovirus vector based gene delivery results in the induction of cytokines including IL-8, which may contribute to the development of inflammatory immune responses. Like other adenoviruses, E1 + E3 deleted porcine adenovirus (PAdV) 3 induces the production of IL-8 in infected cells. In contrast, no IL-8 production could be detected in cells infected with wild-type or mutant PAdV-3s containing deletion in E1A + E3 (PAV211) or E1Bsmall + E3 (PAV212). Expression of PAdV-3 E1Blarge inhibited the NF-kappaB dependent transcription of luciferase from IL-8 promoter. Imunofluorescence and electrophoretic mobility shift assays suggested that constitutive expression of PAdV-3 E1Blarge inhibited the nuclear translocation of NF-kappaB and its subsequent binding to DNA. These results suggest that E1Blarge interacts with NF-kappaB to prevent transcription and down regulate proinflammatory cytokine IL-8 production.  (+info)

*  Porcine adenovirus
Adenoviridae Bovine adenovirus Porcine Adenovirus, reviewed and published by WikiVet at http://en.wikivet.net/Porcine_ ... Porcine adenovirus (aka pADV 1-5 or pADV A-C) is a member of the adenoviridae family. It causes gastrointestinal disease in ... young pigs and is thought to contribute to multifactorial porcine respiratory diseases complexes. Several strains of the virus ...
*  List of MeSH codes (B04)
... adenoviruses, canine MeSH B04.280.030.500.350 --- adenoviruses, human MeSH B04.280.030.500.675 --- adenoviruses, porcine MeSH ... adenoviruses, human MeSH B04.909.204.097.500.675 --- adenoviruses, porcine MeSH B04.909.204.097.500.700 --- adenoviruses, ... fowl adenovirus a MeSH B04.909.204.097.500 --- mastadenovirus MeSH B04.909.204.097.500.200 --- adenoviruses, canine MeSH ... porcine MeSH B04.280.640.615 --- polyomavirus MeSH B04.280.640.615.100 --- bk virus MeSH B04.280.640.615.400 --- jc virus MeSH ...
*  Cyclin A2
Preclinical trials involving injections of adenovirus which contained the Ccna2 gene into infarcted porcine (pig) hearts has ... Li Y, Graham C, Lacy S, Duncan AM, Whyte P (1993). "The adenovirus E1A-associated 130-kD protein is encoded by a member of the ... Faha B, Ewen ME, Tsai LH, Livingston DM, Harlow E (1992). "Interaction between human cyclin A and adenovirus E1A-associated ...
*  CRISPR
... adenovirus (AdV) and adeno-associated virus (AAV). CRISPRs have been used to cut five to 62 genes at once: pig cells have been ... engineered to inactivate all 62 Porcine Endogenous Retroviruses in the pig genome, which eliminated transinfection from the pig ...
*  Mastadenovirus
serotype 14: can cause potentially fatal adenovirus infections. Canine adenovirus 1 (CAdV-1) can lead to death in puppies, or ... Murine mastadenovirus C Ovine mastadenovirus A Ovine mastadenovirus B Porcine mastadenovirus A Porcine mastadenovirus B Porcine ...
*  Adenoviridae
Adenoviruses Stanford University - Adenoviruses Adenoviruses General Concepts General information on Adenovirus DNA virus ... Murine mastadenovirus C Ovine mastadenovirus A Ovine mastadenovirus B Porcine mastadenovirus A Porcine mastadenovirus B Porcine ... Tupaia adenovirus (TAV) (Tree shrew adenovirus 1) has been isolated from tree shrews. Otarine adenovirus 1 has been isolated ... In babies, adenoviruses can also cause coughing fits that look almost exactly like whooping cough. Adenoviruses can also cause ...
*  FOXO1
A study detects that FOXO1 regulates the nuclear localization of p27KIP1 in porcine granulosa cells and impacts cell cycle ... which cause FOXO1-induced apoptosis in the human prostate cancer cell line LAPC4 when FOXO1 adenovirus-mediated overexpression ...
*  Progressive inflammatory neuropathy
... adenoviruses, hepatitis A, and hepatitis E. They also tried to identify bacteria such as salmonella and escherichia coli (e. ... "Auto-immune polyradiculoneuropathy and a novel IgG biomarker in workers exposed to aerosolized porcine brain". Journal of the ... "An outbreak of neurological autoimmunity with polyradiculoneuropathy in workers exposed to aerosolised porcine neural tissue: a ... "Outbreak of Progressive Inflammatory Neuropathy Following Exposure to Aerosolized Porcine Neural Tissue". Mount Sinai Journal ...
*  DNA virus
Another virus - porcine stool associated virus 4 - has been isolated. It appears to be related to the fur seal virus. Two ... Benson SD, Bamford JK, Bamford DH, Burnett RM (1999). "Viral evolution revealed by bacteriophage PRD1 and human adenovirus coat ... Meehan BM, Creelan JL, McNulty MS, Todd D (1997). "Sequence of porcine circovirus DNA: affinities with plant circoviruses". J ... Phylogenetic analysis of these genes places the adenoviruses (Adenoviridae), bacteriophages (Caudovirales) and the plant and ...
*  Parvovirus
Porcine parvovirus causes a reproductive disease in swine known as SMEDI, which stands for stillbirth, mummification, embryonic ... which only work with a helper virus such as adenovirus. Other viruses that can infect without helper viruses are called as ...
*  List of MeSH codes (C02)
... adenovirus infections, human MeSH C02.256.076.381 --- hepatitis, infectious canine MeSH C02.256.430.400 --- hepatitis b MeSH ... enzootic porcine MeSH C02.782.687.359.500 --- hepatitis a MeSH C02.782.687.359.764 --- poliomyelitis MeSH C02.782.687.359. ... porcine reproductive and respiratory syndrome MeSH C02.782.600.550 --- coronaviridae infections MeSH C02.782.600.550.200 --- ...
*  Human HGF plasmid DNA therapy
A phase I clinical trial entailed injecting an adenovirus vector with the human HGF (Ad-hHGF) gene into the coronary vessels ... "Angiogenic and antifibrotic actions of hepatocyte growth factor improve cardiac dysfunction in porcine ischemic cardiomyopathy ...
*  Human herpesvirus 7
Widen, B. F.; Lowings, J. P.; Belak, S.; Banks, M. (August 1999). "Development of a PCR system for porcine cytomegalovirus ... "Human herpesvirus 7 infection of lymphoid and myeloid cell lines transduced with an adenovirus vector containing the CD4 gene ...
*  L-gulonolactone oxidase
... was mapped to human chromosome 8p21 that corresponds to an evolutionarily conserved segment on either porcine chromosome 4 ( ... mice by helper-dependent adenovirus-based expression of gulonolactone oxidase". Hum. Gene Ther. 19 (12): 1349-58. doi:10.1089/ ...
*  Cell culture
Novel ideas in the field include recombinant DNA-based vaccines, such as one made using human adenovirus (a common cold virus) ... and porcine serum. One complication of these blood-derived ingredients is the potential for contamination of the culture with ... "Protection of mice and poultry from lethal H5N1 avian influenza virus through adenovirus-based immunization". Journal of ...
*  Human bocavirus
... respiratory syncytial virus and adenoviruses. The name bocavirus is derived from bovine and canine, referring to the two known ... "Co-existence of multiple strains of two novel porcine bocaviruses in the same pig, a previously undescribed phenomenon in ... "Complete coding sequences and phylogenetic analysis of porcine bocavirus". J. Gen. Virol. 92 (Pt 4): 784-8. doi:10.1099/vir. ...
Porcine adenovirus - Wikipedia  Porcine adenovirus - Wikipedia
Adenoviridae Bovine adenovirus Porcine Adenovirus, reviewed and published by WikiVet at http://en.wikivet.net/Porcine_ ... Porcine adenovirus (aka pADV 1-5 or pADV A-C) is a member of the adenoviridae family. It causes gastrointestinal disease in ... young pigs and is thought to contribute to multifactorial porcine respiratory diseases complexes. Several strains of the virus ...
more infohttps://en.wikipedia.org/wiki/Porcine_adenovirus
Detection of Bovine and Porcine Adenoviruses for Tracing the Source of Fecal Contamination | Applied and Environmental...  Detection of Bovine and Porcine Adenoviruses for Tracing the Source of Fecal Contamination | Applied and Environmental...
51 human adenovirus (HAd), 5 porcine adenovirus (PAV), and 6 ovine adenovirus (OAV) serotypes. Bovine adenoviruses (BAVs) have ... The data also provided information on the environmental prevalence of animal adenoviruses. Porcine adenoviruses were detected ... one to detect porcine adenoviruses and the other to detect bovine and ovine adenoviruses. The specificity of the assays was ... Detection of Bovine and Porcine Adenoviruses for Tracing the Source of Fecal Contamination. Carlos Maluquer de Motes, Pilar ...
more infohttps://aem.asm.org/content/70/3/1448?ijkey=71b47209489402450b6738e1950dd6368cf98cb5&keytype2=tf_ipsecsha
Porcine adenovirus PCR test  Porcine adenovirus PCR test
Porcine adenovirus. Test code: S0169 - Ultrasensitive qualitative detection of common serotypes of porcine adenovirus by real ... Porcine adenovirus is a double-stranded DNA virus belonging to the Adenoviridae family. Infection of swine with this virus ... Help ensure that animal groups and populations are free of porcine adenovirus. ... Elazhary, M.A., Dea, S., Mittal, K.R. and Higgins, R. (1985) Prevalence of Antibodies to Swine Influenza Virus, Porcine ...
more infohttp://zoologix.com/avian/Datasheets/PorcineAdenovirus.htm
3D4/2  ATCC ® CRL-2845™ Sus scrofa lung  3D4/2 ATCC ® CRL-2845™ Sus scrofa lung
... was established in December of 1998 following transfection of primary porcine alveolar macrophage cultures with the pSV3neo ... Porcine adenovirus Herpes simplex virus 1 African swine fever virus Pseudorabies virus Vaccinia virus Swine vesicular disease ... These porcine myelomonocytic cell lines may have a wide variety of applications in porcine virology and immunology Ref. ... The parental porcine monomyeloid cell line, 3D4, was established in December of 1998 following transfection of primary porcine ...
more infohttps://www.atcc.org/en/Products/All/CRL-2845.aspx
Proteins matched: G3DSA:2.60.90.10 (G3DSA:2.60.90.10) | InterPro | EMBL-EBI  Proteins matched: G3DSA:2.60.90.10 (G3DSA:2.60.90.10) | InterPro | EMBL-EBI
Canine adenovirus serotype 1 (strain CLL) (CAdV-1). Loading... Q83457 Fiber protein. Porcine adenovirus A serotype 3 (PAdV-3). ... Canine adenovirus serotype 2 (strain Toronto A 26-61) (CAdV-2). Loading... ...
more infohttp://www.ebi.ac.uk/interpro/signature/G3DSA:2.60.90.10/proteins-matched?start=20
Adenovirus | definition of adenovirus by Medical dictionary  Adenovirus | definition of adenovirus by Medical dictionary
... adenovirus explanation free. What is adenovirus? Meaning of adenovirus medical term. What does adenovirus mean? ... Looking for online definition of adenovirus in the Medical Dictionary? ... porcine adenovirus types 1-4. cause respiratory disease; type 4 causes diarrhea and/or meningitis. ... Related to adenovirus: Canine adenovirus. adenovirus. [ad´ĕ-no-vi″rus] any of a large group of viruses causing disease of the ...
more infohttp://medical-dictionary.thefreedictionary.com/adenovirus
Packaging of viral RNAs in virions of adenoviruses | Virology Journal | Full Text  Packaging of viral RNAs in virions of adenoviruses | Virology Journal | Full Text
Taken together, our data suggest that the viral RNAs may be passively packaged in adenovirus virion during encapsidation of ... Moreover, detection of viral RNA packaged in CsCl purified human adenovirus (HAdV) -5 virions indicates that the viral RNA ... we detected viral RNAs packaged in the porcine adenovirus (PAdV) -3 virions. Using Southern blot analysis, we further ... Reddy PS, Idamakanti N, Hyun B-H, Tikoo SK, Babiuk LA: Development of porcine adenovirus-3 as an expression vector. J Gen Virol ...
more infohttps://virologyj.biomedcentral.com/articles/10.1186/1743-422X-6-16
Imbroglios of Viral Taxonomy: Genetic Exchange and Failings of Phenetic Approaches | Journal of Bacteriology  Imbroglios of Viral Taxonomy: Genetic Exchange and Failings of Phenetic Approaches | Journal of Bacteriology
Sequence analysis of porcine adenovirus serotype 5 fibre gene: evidence for recombination. Virus Genes 24:181-185. ... Also, adenoviruses show a clear similarity to the bacteriophages exemplified by E. coli phage PRD1 (9), Pseudomonas phage φ6 of ... Viral evolution revealed by bacteriophage PRD1 and human adenovirus coat protein structures. Cell 98:825-833. ...
more infohttps://jb.asm.org/content/184/17/4891
SMART: GLECT domain annotation  SMART: GLECT domain annotation
Galectin domain of porcine adenovirus type 4 NADC-1 isolate fibre. 2wsv. Galectin domain of porcine adenovirus type 4 NADC-1 ... Galectin domain of porcine adenovirus type 4 NADC-1 isolate fibre complexed with tri(N-acetyl-lactosamine). ... Galectin domain of porcine adenovirus type 4 NADC-1 isolate fibre complexed with lacto-N-neo-tetraose. ... Galectin domain of porcine adenovirus type 4 NADC-1 isolate fibre complexed with N-acetyl-lactosamine. ...
more infohttp://smart.embl-heidelberg.de/smart/do_annotation.pl?BLAST=DUMMY&DOMAIN=GLECT
Assessment of swine-specific bacteriophages of Bacteroides fragilis in swine farms with different antibiotic practices |...  Assessment of swine-specific bacteriophages of Bacteroides fragilis in swine farms with different antibiotic practices |...
... and porcine adenovirus types 3 and 5 (Wolf et al. 2010). However, for less-developed countries, culture techniques that are low ... Use of eukaryotic mitochondrial DNA to differentiate human, bovine, porcine and ovine sources in fecally contaminated surface ...
more infohttps://iwaponline.com/jwh/article/15/2/251/28264/Assessment-of-swine-specific-bacteriophages-of
Structural Analysis of the Evolutionary Origins of Influenza Virus Hemagglutinin and Other Viral Lectins | Journal of Virology  Structural Analysis of the Evolutionary Origins of Influenza Virus Hemagglutinin and Other Viral Lectins | Journal of Virology
Crystallographic structure of porcine adenovirus type 4 fiber head and galectin domains. J. Virol. 84:10558-10568. ... 2B), between the dimer interface in adenovirus GD (Fig. 2C), and in a pocket on one side of the β-sandwich core in influenza ... Among them, rotavirus VP4, adenovirus GD, and coronavirus spike NTD have been shown to share the same structural folds with ... Among them, the β-sandwich cores of rotavirus VP4, adenovirus GD, and coronavirus spike NTD have the same structural topologies ...
more infohttps://jvi.asm.org/content/87/7/4118
Suresh Mittal - College of Veterinary Medicine - Purdue University  Suresh Mittal - College of Veterinary Medicine - Purdue University
Bovine adenovirus type 3 internalization is independent of primary receptors of human adenovirus type 5 and porcine adenovirus ... Bangari, D. S. and Mittal, S. K. (2005). Porcine adenovirus serotype 3 internalization is independent of CAR and avb3 or avb5 ... Aggarwal, N. and Mittal, S. K. (2000). Sequence analyses of porcine adenovirus type 3 E1 region, pIX, and pIVa2 genes. ... Bangari, D. S. and Mittal, S. K. (2004). Porcine adenoviral vectors evade preexisting humoral immunity to adenoviruses and ...
more infohttps://vet.purdue.edu/directory/person.php?id=82
Frontiers | Firewalls Prevent Systemic Dissemination of Vectors Derived from Human Adenovirus Type 5 and Suppress Production of...  Frontiers | Firewalls Prevent Systemic Dissemination of Vectors Derived from Human Adenovirus Type 5 and Suppress Production of...
... we tracked vectors derived from the human adenovirus type 5 at whole body, tissue and cellular scales throughout the digestive ... we tracked vectors derived from the human adenovirus type 5 at whole body, tissue and cellular scales throughout the digestive ... Tuboly, T., and Nagy, E. (2001). Construction and characterization of recombinant porcine adenovirus serotype 5 expressing the ... challenge with classical swine fever following oral or subcutaneous vaccination with a recombinant porcine adenovirus. Virus ...
more infohttps://www.frontiersin.org/articles/10.3389/fcimb.2018.00006/full
Q83467 citations, curated and mapped  Q83467 citations, curated and mapped
"Crystallographic structure of porcine adenovirus type 4 fiber head and galectin domains.". Guardado-Calvo P., Munoz E.M., ... "Sequence analysis of the fiber genomic region of a porcine adenovirus predicts a novel fiber protein.". Kleiboeker S.B.. Virus ... "Crystallization of the head and galectin-like domains of porcine adenovirus isolate NADC-1 fibre.". Guardado-Calvo P., Llamas- ...
more infohttp://www.uniprot.org/uniprot/Q83467/publications
Ramesh  Vemulapalli  Ramesh Vemulapalli
Evaluation of cross-reactive cell-mediated immune responses among human, bovine and porcine adenoviruses. A Sharma, M Tandon, Y ...
more infohttps://vetmed.tamu.edu/vtpb/people/directorydetail?userid=15424
Adenovirus receptors and their implications in gene delivery  Adenovirus receptors and their implications in gene delivery
Bovine adenovirus type 3 internalization is independent of primary receptors of human adenovirus type 5 and porcine adenovirus ... Vectors based on nonhuman Ads originally derived from pig (porcine adenovirus serotype 3; PAd3) or cattle (bovine adenovirus ... Porcine adenovirus-3 as a helper-dependent expression vector. J. Gen. Virol. 1999a;80:2909-2916. [PubMed] ... Porcine adenovirus serotype 3 internalization is independent of CAR and alphavbeta3 or alphavbeta5 integrin. Virology. 2005;332 ...
more infohttp://pubmedcentralcanada.ca/pmcc/articles/PMC2903974/
ASMscience | Fecal Pollution, Public  ASMscience | Fecal Pollution, Public
Detection of bovine and porcine adenoviruses for tracing the source of fecal contamination. Appl. Environ. Microbiol. 70:1448- ... Jiang, S.,, R. Noble, and, W. Chu. 2001. Human adenoviruses and coliphages in urban runoff-impacted coastal waters of Southern ... porcine and ovine sources in fecally contaminated surface water. Water Res. 39:541-548.. ... Teschoviruses as indicators of porcine fecal contamination of surface water. Appl. Environ. Microbiol. 69:6311-6315.. ...
more infohttp://www.asmscience.org/content/book/10.1128/9781555815769.ch01
Goedegebuure SP[au] - PubMed - NCBI  Goedegebuure SP[au] - PubMed - NCBI
Incorporation of porcine adenovirus 4 fiber protein enhances infectivity of adenovirus vector on dendritic cells: implications ... Development of an adenovirus vector vaccine platform for targeting dendritic cells.. Sharma PK, Dmitriev IP, Kashentseva EA, ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed?cmd=search&term=Goedegebuure+SP%5Bau%5D&dispmax=50
Welcome to CDC stacks | Hepatitis E Virus in Pork Production Chain in Czech Republic, Italy, and Spain, 2010 - 18685 | Emerging...  Welcome to CDC stacks | Hepatitis E Virus in Pork Production Chain in Czech Republic, Italy, and Spain, 2010 - 18685 | Emerging...
Indicator virus (porcine adenovirus) was ubiquitous in fecal samples and absent in liver samples and was detected in 1 ... At point of sale, we found porcine adenovirus in sausages (1%-2%). The possible dissemination of HEV and other fecal viruses ...
more infohttps://stacks.cdc.gov/view/cdc/18685
  • These sets of primers produced negative results in nested PCR assays when human adenovirus controls and urban-sewage samples were tested. (asm.org)
  • We characterized their SLA genes and subsequently performed peptide-SLA binding assays using a peptide from porcine circovirus type 2 open reading frame 2 to experimentally measure the binding affinity of the peptide to SLA class II. (biomedcentral.com)
  • The sensitivity of this nested PCR test was previously established to be as high as one viral particle, and the same procedure has been applied to the detection of animal adenoviruses. (asm.org)
  • In the present study, two sets of oligonucleotides were designed, one for the specific detection of BAV and OAV and the other for the specific detection of PAV, to evaluate the level of excretion of adenoviruses by farm animals and to devise a test to trace the specific source of viral contamination in the environment. (asm.org)
  • Moreover, detection of viral RNA packaged in CsCl purified human adenovirus (HAdV) -5 virions indicates that the viral RNA packaging might be a common phenomenon in members of Adenoviridae family. (biomedcentral.com)
  • Taken together, our data suggest that the viral RNAs may be passively packaged in adenovirus virion during encapsidation of viral genomic DNA in cell nuclei. (biomedcentral.com)
  • To date, crystal structures have been determined for the following viral lectins: rotavirus VP4 ( 2 ), adenovirus galectin domain (GD) ( 3 ), coronavirus spike protein N-terminal domain (NTD) ( 4 , 5 ), coronavirus hemagglutinin-esterase (HE) ( 6 ), and torovirus hemagglutinin-esterase ( 7 ), in addition to influenza virus HA. (asm.org)
  • The parental porcine monomyeloid cell line, 3D4, was established in December of 1998 following transfection of primary porcine alveolar macrophage cultures with the pSV3neo plasmid. (atcc.org)