Species of the genus MASTADENOVIRUS, causing a wide range of diseases in humans. Infections are mostly asymptomatic, but can be associated with diseases of the respiratory, ocular, and gastrointestinal systems. Serotypes (named with Arabic numbers) have been grouped into species designated Human adenovirus A-F.
A family of non-enveloped viruses infecting mammals (MASTADENOVIRUS) and birds (AVIADENOVIRUS) or both (ATADENOVIRUS). Infections may be asymptomatic or result in a variety of diseases.
Respiratory and conjunctival infections caused by 33 identified serotypes of human adenoviruses.
Proteins transcribed from the E1A genome region of ADENOVIRUSES which are involved in positive regulation of transcription of the early genes of host infection.
Virus diseases caused by the ADENOVIRIDAE.
Proteins encoded by adenoviruses that are synthesized prior to, and in the absence of, viral DNA replication. The proteins are involved in both positive and negative regulation of expression in viral and cellular genes, and also affect the stability of viral mRNA. Some are also involved in oncogenic transformation.
Proteins transcribed from the E1B region of ADENOVIRUSES which are involved in regulation of the levels of early and late viral gene expression.
Proteins transcribed from the E3 region of ADENOVIRUSES but not essential for viral replication. The E3 19K protein mediates adenovirus persistence by reducing the expression of class I major histocompatibility complex antigens on the surface of infected cells.
Proteins transcribed from the E4 region of ADENOVIRUSES. The E4 19K protein transactivates transcription of the adenovirus E2F protein and complexes with it.
The very first viral gene products synthesized after cells are infected with adenovirus. The E1 region of the genome has been divided into two major transcriptional units, E1A and E1B, each expressing proteins of the same name (ADENOVIRUS E1A PROTEINS and ADENOVIRUS E1B PROTEINS).
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
Species of the genus MASTADENOVIRUS that causes fever, edema, vomiting, and diarrhea in dogs and encephalitis in foxes. Epizootics have also been caused in bears, wolves, coyotes, and skunks. The official species name is Canine adenovirus and it contains two serotypes.
Proteins transcribed from the E2 region of ADENOVIRUSES. Several of these are required for viral DNA replication.
A genus of ADENOVIRIDAE that infects MAMMALS including humans and causes a wide range of diseases. The type species is Human adenovirus C (see ADENOVIRUSES, HUMAN).
Species of the genus MASTADENOVIRUS, causing neurological disease in pigs.
A genus of ADENOVIRIDAE that infects birds. The type species is FOWL ADENOVIRUS A.
Techniques and strategies which include the use of coding sequences and other conventional or radical means to transform or modify cells for the purpose of treating or reversing disease conditions.
The type species of the genus AVIADENOVIRUS, family ADENOVIRIDAE, an oncogenic virus of birds. This is also called CELO virus for chick embryo lethal orphan virus.
Deoxyribonucleic acid that makes up the genetic material of viruses.
The introduction of functional (usually cloned) GENES into cells. A variety of techniques and naturally occurring processes are used for the gene transfer such as cell hybridization, LIPOSOMES or microcell-mediated gene transfer, ELECTROPORATION, chromosome-mediated gene transfer, TRANSFECTION, and GENETIC TRANSDUCTION. Gene transfer may result in genetically transformed cells and individual organisms.
Proteins that form the CAPSID of VIRUSES.
The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
Established cell cultures that have the potential to propagate indefinitely.
The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.
Specific molecular components of the cell capable of recognizing and interacting with a virus, and which, after binding it, are capable of generating some signal that initiates the chain of events leading to the biological response.
Use of attenuated VIRUSES as ANTINEOPLASTIC AGENTS to selectively kill CANCER cells.
Tumor-selective, replication competent VIRUSES that have antineoplastic effects. This is achieved by producing cytotoxicity-enhancing proteins and/or eliciting an antitumor immune response. They are genetically engineered so that they can replicate in CANCER cells but not in normal cells, and are used in ONCOLYTIC VIROTHERAPY.
Simultaneous inflammation of the cornea and conjunctiva.
The transfer of bacterial DNA by phages from an infected bacterium to another bacterium. This also refers to the transfer of genes into eukaryotic cells by viruses. This naturally occurring process is routinely employed as a GENE TRANSFER TECHNIQUE.
Proteins found in any species of virus.
The functional hereditary units of VIRUSES.
Products of viral oncogenes, most commonly retroviral oncogenes. They usually have transforming and often protein kinase activities.
Inflammation, often mild, of the conjunctiva caused by a variety of viral agents. Conjunctival involvement may be part of a systemic infection.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Vaccines used to prevent infection by any virus from the family ADENOVIRIDAE.
The outer protein protective shell of a virus, which protects the viral nucleic acid.
An inheritable change in cells manifested by changes in cell division and growth and alterations in cell surface properties. It is induced by infection with a transforming virus.
Genes that are introduced into an organism using GENE TRANSFER TECHNIQUES.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Visible morphologic changes in cells infected with viruses. It includes shutdown of cellular RNA and protein synthesis, cell fusion, release of lysosomal enzymes, changes in cell membrane permeability, diffuse changes in intracellular structures, presence of viral inclusion bodies, and chromosomal aberrations. It excludes malignant transformation, which is CELL TRANSFORMATION, VIRAL. Viral cytopathogenic effects provide a valuable method for identifying and classifying the infecting viruses.
Viruses which enable defective viruses to replicate or to form a protein coat by complementing the missing gene function of the defective (satellite) virus. Helper and satellite may be of the same or different genus.
Viruses which lack a complete genome so that they cannot completely replicate or cannot form a protein coat. Some are host-dependent defectives, meaning they can replicate only in cell systems which provide the particular genetic function which they lack. Others, called SATELLITE VIRUSES, are able to replicate only when their genetic defect is complemented by a helper virus.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.
Ribonucleic acid that makes up the genetic material of viruses.
Any of the processes by which cytoplasmic factors influence the differential control of gene action in viruses.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
A genus of the family PICORNAVIRIDAE whose members preferentially inhabit the intestinal tract of a variety of hosts. The genus contains many species. Newly described members of human enteroviruses are assigned continuous numbers with the species designated "human enterovirus".
The process by which a DNA molecule is duplicated.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
A genus of ADENOVIRIDAE that comprises viruses of several species of MAMMALS and BIRDS. The type species is Ovine adenovirus D.
A species of POLYOMAVIRUS originally isolated from Rhesus monkey kidney tissue. It produces malignancy in human and newborn hamster kidney cell cultures.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
Mutant mice homozygous for the recessive gene "nude" which fail to develop a thymus. They are useful in tumor studies and studies on immune responses.
Process of growing viruses in live animals, plants, or cultured cells.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
The complete genetic complement contained in a DNA or RNA molecule in a virus.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
A cell line derived from cultured tumor cells.
Invasion of the host RESPIRATORY SYSTEM by microorganisms, usually leading to pathological processes or diseases.
Production of new arrangements of DNA by various mechanisms such as assortment and segregation, CROSSING OVER; GENE CONVERSION; GENETIC TRANSFORMATION; GENETIC CONJUGATION; GENETIC TRANSDUCTION; or mixed infection of viruses.
Immunoglobulins produced in response to VIRAL ANTIGENS.
Minute infectious agents whose genomes are composed of DNA or RNA, but not both. They are characterized by a lack of independent metabolism and the inability to replicate outside living host cells.
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Those proteins recognized by antibodies from serum of animals bearing tumors induced by viruses; these proteins are presumably coded for by the nucleic acids of the same viruses that caused the neoplastic transformation.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
Process of determining and distinguishing species of bacteria or viruses based on antigens they share.
An area showing altered staining behavior in the nucleus or cytoplasm of a virus-infected cell. Some inclusion bodies represent "virus factories" in which viral nucleic acid or protein is being synthesized; others are merely artifacts of fixation and staining. One example, Negri bodies, are found in the cytoplasm or processes of nerve cells in animals that have died from rabies.
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
Inflammation of the lung parenchyma that is caused by a viral infection.
A suborder of PRIMATES consisting of six families: CEBIDAE (some New World monkeys), ATELIDAE (some New World monkeys), CERCOPITHECIDAE (Old World monkeys), HYLOBATIDAE (gibbons and siamangs), CALLITRICHINAE (marmosets and tamarins), and HOMINIDAE (humans and great apes).
Substances elaborated by viruses that have antigenic activity.
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
A ubiquitously expressed complement receptor that binds COMPLEMENT C3B and COMPLEMENT C4B and serves as a cofactor for their inactivation. CD46 also interacts with a wide variety of pathogens and mediates immune response.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Eukaryotic cell line obtained in a quiescent or stationary phase which undergoes conversion to a state of unregulated growth in culture, resembling an in vitro tumor. It occurs spontaneously or through interaction with viruses, oncogenes, radiation, or drugs/chemicals.
A genus of the family PARVOVIRIDAE, subfamily PARVOVIRINAE, which are dependent on a coinfection with helper adenoviruses or herpesviruses for their efficient replication. The type species is Adeno-associated virus 2.
Suspensions of attenuated or killed viruses administered for the prevention or treatment of infectious viral disease.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
INFLAMMATION of any segment of the GASTROINTESTINAL TRACT from ESOPHAGUS to RECTUM. Causes of gastroenteritis are many including genetic, infection, HYPERSENSITIVITY, drug effects, and CANCER.
This line KB is now known to be a subline of the ubiquitous KERATIN-forming tumor cell line HeLa. It was originally thought to be derived from an epidermal carcinoma of the mouth, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via contamination by HELA CELLS. The cells are positive for keratin by immunoperoxidase staining. KB cells have been reported to contain human papillomavirus18 (HPV-18) sequences.
Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected.
Viruses that produce tumors.
The directional growth of an organism in response to an external stimulus such as light, touch, or gravity. Growth towards the stimulus is a positive tropism; growth away from the stimulus is a negative tropism. (From Concise Dictionary of Biology, 1990)
Injections introduced directly into localized lesions.
An enzyme that catalyzes the conversion of ATP and thymidine to ADP and thymidine 5'-phosphate. Deoxyuridine can also act as an acceptor and dGTP as a donor. (From Enzyme Nomenclature, 1992) EC 2.7.1.21.
In vivo methods of screening investigative anticancer drugs, biologic response modifiers or radiotherapies. Human tumor tissue or cells are transplanted into mice or rats followed by tumor treatment regimens. A variety of outcomes are monitored to assess antitumor effectiveness.
Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH.
The genetic unit consisting of three structural genes, an operator and a regulatory gene. The regulatory gene controls the synthesis of the three structural genes: BETA-GALACTOSIDASE and beta-galactoside permease (involved with the metabolism of lactose), and beta-thiogalactoside acetyltransferase.
A subfamily of the family MURIDAE comprised of 69 genera. New World mice and rats are included in this subfamily.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
Proteins prepared by recombinant DNA technology.
The genetic process of crossbreeding between genetically dissimilar parents to produce a hybrid.
The measurement of infection-blocking titer of ANTISERA by testing a series of dilutions for a given virus-antiserum interaction end-point, which is generally the dilution at which tissue cultures inoculated with the serum-virus mixtures demonstrate cytopathology (CPE) or the dilution at which 50% of test animals injected with serum-virus mixtures show infectivity (ID50) or die (LD50).
Small synthetic peptides that mimic surface antigens of pathogens and are immunogenic, or vaccines manufactured with the aid of recombinant DNA techniques. The latter vaccines may also be whole viruses whose nucleic acids have been modified.
Cell changes manifested by escape from control mechanisms, increased growth potential, alterations in the cell surface, karyotypic abnormalities, morphological and biochemical deviations from the norm, and other attributes conferring the ability to invade, metastasize, and kill.
Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Tumors or cancer of the MOUTH.
Elements of limited time intervals, contributing to particular results or situations.
Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.
A general term for diseases produced by viruses.
Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
Transcription factors that were originally identified as site-specific DNA-binding proteins essential for DNA REPLICATION by ADENOVIRUSES. They play important roles in MAMMARY GLAND function and development.
Experimentally induced new abnormal growth of TISSUES in animals to provide models for studying human neoplasms.
Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
Defective viruses which can multiply only by association with a helper virus which complements the defective gene. Satellite viruses may be associated with certain plant viruses, animal viruses, or bacteriophages. They differ from satellite RNA; (RNA, SATELLITE) in that satellite viruses encode their own coat protein.
The sum of the weight of all the atoms in a molecule.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
Nuclear phosphoprotein encoded by the p53 gene (GENES, P53) whose normal function is to control CELL PROLIFERATION and APOPTOSIS. A mutant or absent p53 protein has been found in LEUKEMIA; OSTEOSARCOMA; LUNG CANCER; and COLORECTAL CANCER.
The infective system of a virus, composed of the viral genome, a protein core, and a protein coat called a capsid, which may be naked or enclosed in a lipoprotein envelope called the peplos.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
Y-box-binding protein 1 was originally identified as a DNA-binding protein that interacts with Y-box PROMOTER REGIONS of MHC CLASS II GENES. It is a highly conserved transcription factor that regulates expression of a wide variety of GENES.
An alpha integrin with a molecular weight of 160-kDa that is found in a variety of cell types. It undergoes posttranslational cleavage into a heavy and a light chain that are connected by disulfide bonds. Integrin alphaV can combine with several different beta subunits to form heterodimers that generally bind to RGD sequence-containing extracellular matrix proteins.
Genes whose expression is easily detectable and therefore used to study promoter activity at many positions in a target genome. In recombinant DNA technology, these genes may be attached to a promoter region of interest.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Proteins which are involved in the phenomenon of light emission in living systems. Included are the "enzymatic" and "non-enzymatic" types of system with or without the presence of oxygen or co-factors.
Deoxycytidine (dihydrogen phosphate). A deoxycytosine nucleotide containing one phosphate group esterified to the deoxyribose moiety in the 2'-,3'- or 5- positions.
Enzymes that oxidize certain LUMINESCENT AGENTS to emit light (PHYSICAL LUMINESCENCE). The luciferases from different organisms have evolved differently so have different structures and substrates.
Method for measuring viral infectivity and multiplication in CULTURED CELLS. Clear lysed areas or plaques develop as the VIRAL PARTICLES are released from the infected cells during incubation. With some VIRUSES, the cells are killed by a cytopathic effect; with others, the infected cells are not killed but can be detected by their hemadsorptive ability. Sometimes the plaque cells contain VIRAL ANTIGENS which can be measured by IMMUNOFLUORESCENCE.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.
The binding of virus particles to receptors on the host cell surface. For enveloped viruses, the virion ligand is usually a surface glycoprotein as is the cellular receptor. For non-enveloped viruses, the virus CAPSID serves as the ligand.
Experimental transplantation of neoplasms in laboratory animals for research purposes.
A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.
Product of the retinoblastoma tumor suppressor gene. It is a nuclear phosphoprotein hypothesized to normally act as an inhibitor of cell proliferation. Rb protein is absent in retinoblastoma cell lines. It also has been shown to form complexes with the adenovirus E1A protein, the SV40 T antigen, and the human papilloma virus E7 protein.
The study of the structure, growth, function, genetics, and reproduction of viruses, and VIRUS DISEASES.
A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.
Vaccines or candidate vaccines containing inactivated HIV or some of its component antigens and designed to prevent or treat AIDS. Some vaccines containing antigens are recombinantly produced.
An ACYCLOVIR analog that is a potent inhibitor of the Herpesvirus family including cytomegalovirus. Ganciclovir is used to treat complications from AIDS-associated cytomegalovirus infections.
The relationships of groups of organisms as reflected by their genetic makeup.
Infections of the eye caused by minute intracellular agents. These infections may lead to severe inflammation in various parts of the eye - conjunctiva, iris, eyelids, etc. Several viruses have been identified as the causative agents. Among these are Herpesvirus, Adenovirus, Poxvirus, and Myxovirus.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
Processes that stimulate the GENETIC TRANSCRIPTION of a gene or set of genes.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
The rate dynamics in chemical or physical systems.
The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990)
Receptors such as INTEGRIN ALPHAVBETA3 that bind VITRONECTIN with high affinity and play a role in cell migration. They also bind FIBRINOGEN; VON WILLEBRAND FACTOR; osteopontin; and THROMBOSPONDINS.
A genus of ADENOVIRIDAE comprising species including viruses of frogs (FROGS AND TOADS) and TURKEYS. The type species is Frog adenovirus.
Directed modification of the gene complement of a living organism by such techniques as altering the DNA, substituting genetic material by means of a virus, transplanting whole nuclei, transplanting cell hybrids, etc.
The integration of exogenous DNA into the genome of an organism at sites where its expression can be suitably controlled. This integration occurs as a result of homologous recombination.
An enzyme that catalyzes the acetylation of chloramphenicol to yield chloramphenicol 3-acetate. Since chloramphenicol 3-acetate does not bind to bacterial ribosomes and is not an inhibitor of peptidyltransferase, the enzyme is responsible for the naturally occurring chloramphenicol resistance in bacteria. The enzyme, for which variants are known, is found in both gram-negative and gram-positive bacteria. EC 2.3.1.28.
Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
A malignant neoplasm made up of epithelial cells tending to infiltrate the surrounding tissues and give rise to metastases. It is a histological type of neoplasm but is often wrongly used as a synonym for "cancer." (From Dorland, 27th ed)
Any immunization following a primary immunization and involving exposure to the same or a closely related antigen.
Macromolecular molds for the synthesis of complementary macromolecules, as in DNA REPLICATION; GENETIC TRANSCRIPTION of DNA to RNA, and GENETIC TRANSLATION of RNA into POLYPEPTIDES.
A member of the p300-CBP transcription factors that was originally identified as a binding partner for ADENOVIRUS E1A PROTEINS.
Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.
A sequence of successive nucleotide triplets that are read as CODONS specifying AMINO ACIDS and begin with an INITIATOR CODON and end with a stop codon (CODON, TERMINATOR).
Polyomavirus antigens which cause infection and cellular transformation. The large T antigen is necessary for the initiation of viral DNA synthesis, repression of transcription of the early region and is responsible in conjunction with the middle T antigen for the transformation of primary cells. Small T antigen is necessary for the completion of the productive infection cycle.
CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). The species is a favorite for cytogenetic studies because of its small chromosome number. The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells.
Inactivation of viruses by non-immune related techniques. They include extremes of pH, HEAT treatment, ultraviolet radiation, IONIZING RADIATION; DESICCATION; ANTISEPTICS; DISINFECTANTS; organic solvents, and DETERGENTS.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.
A fractionated cell extract that maintains a biological function. A subcellular fraction isolated by ultracentrifugation or other separation techniques must first be isolated so that a process can be studied free from all of the complex side reactions that occur in a cell. The cell-free system is therefore widely used in cell biology. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p166)
A species of CERCOPITHECUS containing three subspecies: C. tantalus, C. pygerythrus, and C. sabeus. They are found in the forests and savannah of Africa. The African green monkey (C. pygerythrus) is the natural host of SIMIAN IMMUNODEFICIENCY VIRUS and is used in AIDS research.
Forceful administration into a muscle of liquid medication, nutrient, or other fluid through a hollow needle piercing the muscle and any tissue covering it.
Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.
A critical subpopulation of regulatory T-lymphocytes involved in MHC Class I-restricted interactions. They include both cytotoxic T-lymphocytes (T-LYMPHOCYTES, CYTOTOXIC) and CD8+ suppressor T-lymphocytes.
A genus of the family HERPESVIRIDAE, subfamily BETAHERPESVIRINAE, infecting the salivary glands, liver, spleen, lungs, eyes, and other organs, in which they produce characteristically enlarged cells with intranuclear inclusions. Infection with Cytomegalovirus is also seen as an opportunistic infection in AIDS.
The assembly of VIRAL STRUCTURAL PROTEINS and nucleic acid (VIRAL DNA or VIRAL RNA) to form a VIRUS PARTICLE.
New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms.
Proteins conjugated with deoxyribonucleic acids (DNA) or specific DNA.
Bacteriophage and type species in the genus Tectivirus, family TECTIVIRIDAE. They are specific for Gram-negative bacteria.
A genus of the family HERPESVIRIDAE, subfamily ALPHAHERPESVIRINAE, consisting of herpes simplex-like viruses. The type species is HERPESVIRUS 1, HUMAN.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
Antibodies that reduce or abolish some biological activity of a soluble antigen or infectious agent, usually a virus.
A species of ENTEROVIRUS infecting humans and containing 36 serotypes. It is comprised of all the echoviruses and a few coxsackieviruses, including all of those previously named coxsackievirus B.
Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).
The production of PEPTIDES or PROTEINS by the constituents of a living organism. The biosynthesis of proteins on RIBOSOMES following an RNA template is termed translation (TRANSLATION, GENETIC). There are other, non-ribosomal peptide biosynthesis (PEPTIDE BIOSYNTHESIS, NUCLEIC ACID-INDEPENDENT) mechanisms carried out by PEPTIDE SYNTHASES and PEPTIDYLTRANSFERASES. Further modifications of peptide chains yield functional peptide and protein molecules.
Proteins found mainly in icosahedral DNA and RNA viruses. They consist of proteins directly associated with the nucleic acid inside the NUCLEOCAPSID.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
The entering of cells by viruses following VIRUS ATTACHMENT. This is achieved by ENDOCYTOSIS, by direct MEMBRANE FUSION of the viral membrane with the CELL MEMBRANE, or by translocation of the whole virus across the cell membrane.
Vaccines or candidate vaccines used to prevent EBOLA HEMORRHAGIC FEVER.
Immunized T-lymphocytes which can directly destroy appropriate target cells. These cytotoxic lymphocytes may be generated in vitro in mixed lymphocyte cultures (MLC), in vivo during a graft-versus-host (GVH) reaction, or after immunization with an allograft, tumor cell or virally transformed or chemically modified target cell. The lytic phenomenon is sometimes referred to as cell-mediated lympholysis (CML). These CD8-positive cells are distinct from NATURAL KILLER CELLS and NATURAL KILLER T-CELLS. There are two effector phenotypes: TC1 and TC2.
Cis-acting DNA sequences which can increase transcription of genes. Enhancers can usually function in either orientation and at various distances from a promoter.
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
The top portion of the pharynx situated posterior to the nose and superior to the SOFT PALATE. The nasopharynx is the posterior extension of the nasal cavities and has a respiratory function.
Tumor suppressor genes located on the short arm of human chromosome 17 and coding for the phosphoprotein p53.
A conserved A-T rich sequence which is contained in promoters for RNA polymerase II. The segment is seven base pairs long and the nucleotides most commonly found are TATAAAA.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
A family of basic helix-loop-helix transcription factors that control expression of a variety of GENES involved in CELL CYCLE regulation. E2F transcription factors typically form heterodimeric complexes with TRANSCRIPTION FACTOR DP1 or transcription factor DP2, and they have N-terminal DNA binding and dimerization domains. E2F transcription factors can act as mediators of transcriptional repression or transcriptional activation.

CAR-dependent and CAR-independent pathways of adenovirus vector-mediated gene transfer and expression in human fibroblasts. (1/2914)

Primary fibroblasts are not efficiently transduced by subgroup C adenovirus (Ad) vectors because they express low levels of the high-affinity Coxsackie virus and adenovirus receptor (CAR). In the present study, we have used primary human dermal fibroblasts as a model to explore strategies by which Ad vectors can be designed to enter cells deficient in CAR. Using an Ad vector expressing the human CAR cDNA (AdCAR) at high multiplicity of infection, primary fibroblasts were converted from being CAR deficient to CAR sufficient. Efficiency of subsequent gene transfer by standard Ad5-based vectors and Ad5-based vectors with alterations in penton and fiber was evaluated. Marked enhancement of binding and transgene expression by standard Ad5 vectors was achieved in CAR-sufficient fibroblasts. Expression by AdDeltaRGDbetagal, an Ad5-based vector lacking the arginine-glycine-aspartate (RGD) alphaV integrin recognition site from its penton base, was achieved in CAR-sufficient, but not CAR-deficient, cells. Fiber-altered Ad5-based vectors, including (a) AdF(pK7)betagal (bearing seven lysines on the end of fiber) (b) AdF(RGD)betagal (bearing a high-affinity RGD sequence on the end of fiber), and (c) AdF9sK betagal (bearing a short fiber and Ad9 knob), demonstrated enhanced gene transfer in CAR-deficient fibroblasts, with no further enhancement in CAR-sufficient fibroblasts. Together, these observations demonstrate that CAR deficiency on Ad targets can be circumvented either by supplying CAR or by modifying the Ad fiber to bind to other cell-surface receptors.  (+info)

Reduced phosphorylation of p50 is responsible for diminished NF-kappaB binding to the major histocompatibility complex class I enhancer in adenovirus type 12-transformed cells. (2/2914)

Reduced cell surface levels of major histocompatibility complex class I antigens enable adenovirus type 12 (Ad12)-transformed cells to escape immunosurveillance by cytotoxic T lymphocytes (CTL), contributing to their tumorigenic potential. In contrast, nontumorigenic Ad5-transformed cells harbor significant cell surface levels of class I antigens and are susceptible to CTL lysis. Ad12 E1A mediates down-regulation of class I transcription by increasing COUP-TF repressor binding and decreasing NF-kappaB activator binding to the class I enhancer. The mechanism underlying the decreased binding of nuclear NF-kappaB in Ad12-transformed cells was investigated. Electrophoretic mobility shift assay analysis of hybrid NF-kappaB dimers reconstituted from denatured and renatured p50 and p65 subunits from Ad12- and Ad5-transformed cell nuclear extracts demonstrated that p50, and not p65, is responsible for the decreased ability of NF-kappaB to bind to DNA in Ad12-transformed cells. Hypophosphorylation of p50 was found to correlate with restricted binding of NF-kappaB to DNA in Ad12-transformed cells. The importance of phosphorylation of p50 for NF-kappaB binding was further demonstrated by showing that an NF-kappaB dimer composed of p65 and alkaline phosphatase-treated p50 from Ad5-transformed cell nuclear extracts could not bind to DNA. These results suggest that phosphorylation of p50 is a key step in the nuclear regulation of NF-kappaB in adenovirus-transformed cells.  (+info)

Microtubule-dependent plus- and minus end-directed motilities are competing processes for nuclear targeting of adenovirus. (3/2914)

Adenovirus (Ad) enters target cells by receptor-mediated endocytosis, escapes to the cytosol, and then delivers its DNA genome into the nucleus. Here we analyzed the trafficking of fluorophore-tagged viruses in HeLa and TC7 cells by time-lapse microscopy. Our results show that native or taxol-stabilized microtubules (MTs) support alternating minus- and plus end-directed movements of cytosolic virus with elementary speeds up to 2.6 micrometer/s. No directed movement was observed in nocodazole-treated cells. Switching between plus- and minus end-directed elementary speeds at frequencies up to 1 Hz was observed in the periphery and near the MT organizing center (MTOC) after recovery from nocodazole treatment. MT-dependent motilities allowed virus accumulation near the MTOC at population speeds of 1-10 micrometer/min, depending on the cell type. Overexpression of p50/dynamitin, which is known to affect dynein-dependent minus end-directed vesicular transport, significantly reduced the extent and the frequency of minus end-directed migration of cytosolic virus, and increased the frequency, but not the extent of plus end-directed motility. The data imply that a single cytosolic Ad particle engages with two types of MT-dependent motor activities, the minus end- directed cytoplasmic dynein and an unknown plus end- directed activity.  (+info)

Differences in the interactions of oncogenic adenovirus 12 early region 1A and nononcogenic adenovirus 2 early region 1A with the cellular coactivators p300 and CBP. (4/2914)

Association with the cellular coactivators p300 and CBP is required for the growth-regulatory function of adenoviral (Ad) early region 1A (E1A) proteins. E1A regions necessary for these interactions overlap with domains involved in the induction of tumours in immunocompetent rodents through highly oncogenic Ad12. Differences in the association of cellular factors with the respective E1A domains of Ad12 and nononcogenic Ad2 might therefore be involved in serotype-specific oncogenicity. We analyzed the interaction of the Ad12 E1A 235R protein with p300 and CBP. Here we demonstrate that in the case of Ad12, but not Ad2/5, amino acids (aa) 1-29 of E1A proteins are sufficient to bind the p300-C/H3 domain in vivo and wild-type p300 in vitro. The conserved arginine-2, which is essential for the interaction between Ad2 E1A and p300, was dispensable for the Ad12 E1A 235R-p300 interaction in vitro. In addition to the p300-C/H3 region, we identified a second domain within p300 (aa 1999-2200) binding to the 235R protein. Contrary to p300, the amino-terminus and CR1 are necessary to associate with CBP. The aa 1-29 of the 235R protein but not CR1 are essential for the repression of colTRE-driven gene expression. This repression function is strictly dependent on p300 but not on CBP.  (+info)

Evidence for an adenovirus type 2-coded early glycoprotein. (5/2914)

We have identified an adenovirus type 2 (Ad2)-induced early glycopolypeptide with an apparent molecular weight of 20,000 to 21,000 (20/21K), as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The 20/21K polypeptide could be labeled in vivo with [(3)H]glucosamine. [(35)S]methionine- and [(3)H]-glucosamine-labeled 20/21K polypeptides bound to concanavalin A-Sepharose columns and were eluted with 0.2 M methyl-alpha-d-mannoside. The pulse-labeled polypeptide appeared as a sharp band with an apparent molecular weight of 21K, but after a chase it converted to multiple bands with an average molecular weight of 20K. This variability in electrophoretic mobility is consistent with glycosylation or deglycosylation of the 20/21K polypeptide. Analysis of the pulse and pulse-chase-labeled forms by using partial proteolysis indicated that the polypeptides were highly related chemically, but not identical. Most of the 20/21K polypeptide is localized in the cytoplasm fraction of infected cells lysed by Nonidet P-40. The 20/21K polypeptide and a 44K polypeptide, labeled with [(35)S]methionine or [(3)H]glucosamine in Ad2-infected human cells, were precipitated by a rat antiserum against an Ad2-transformed rat cell line (T2C4), but not by antisera against three other Ad2-transformed rat cell lines, or by serum from nonimmune rats. The partial proteolysis patterns of the 20/21K and the 44K polypeptides were indistinguishable, indicating that the two polypeptides are highly related, and suggesting that the 44K polypeptide might be a dimer of the 20/21K polypeptide. The 20/21K polypeptide was also induced in Ad2-early infected monkey and hamster cells. These results imply that the 20/21K polypeptide is synthesized in Ad2-infected human, monkey, and hamster cells, and in one but not all Ad2-transformed rat cells. Thus, the 20/21K polypeptide is probably viral coded rather than cell coded and viral induced.  (+info)

Development and use of a 293 cell line expressing lac repressor for the rescue of recombinant adenoviruses expressing high levels of rabies virus glycoprotein. (6/2914)

An expression cassette designed for high-level production of rabies virus glycoprotein (RG) could not be rescued into a replication-defective, adenovirus-based vector using standard procedures. To overcome this difficulty, a 293-based cell line, designated 293LAP13, was constructed that contained and expressed a derivative of the lac repressor protein. The lac operator sequence, to which the repressor binds, was incorporated into an expression cassette, containing a promoter and intron, designed for high-level production of RG. Insertion of a single operator sequence immediately downstream of the transcription start site and the use of the 293LAP13 cell line allowed recombinant viruses that could not be isolated with 293 cells to be rescued efficiently. The operator-containing virus reached higher titres in 293LAP13 than in parental 293 cells and also produced plaques more efficiently in 293LAP13 cells. Moreover, in non-complementing human and canine cell lines, adenovirus vectors with a promoter-intron expression cassette expressed RG at much higher levels than vectors lacking the intron. These observations, together with the demonstration that expression of RG by operator-containing vectors was repressed markedly in 293LAP13 cells and that this inhibition was relieved at least partly by IPTG, suggest that the 293LAP13 cell line may be useful for the rescue and propagation of many vectors in which high expression of the desired protein prevents vector rescue in 293 cells.  (+info)

The adenoviral E1A oncoproteins interfere with the growth-inhibiting effect of the cdk-inhibitor p21(CIP1/WAF1). (7/2914)

The cdk-inhibitor p21(CIP1/WAF1) inhibits the activities of cyclin-dependent kinases and proliferating cell nuclear antigen, thereby repressing cell-cycle progression and DNA replication. Transforming oncogenes, such as E1A of human adenovirus 5 (Ad5), may interfere with such growth-inhibitory proteins. In this study, we show that in various Ad5E1-transformed cells, p21(CIP1/WAF1) is expressed and that, in general, expression is not downregulated. In addition, colony-formation assays show that in Ad5E1-transformed cells highly overexpressed p21(CIP1/WAF1) can still cause growth inhibition. FACS experiments indicate, however, that a G1 arrest induced by moderate overexpression of p21(CIP1/WAF1) can be overcome by E1A. The E1A proteins may interfere with the function of p21(CIP1/WAF1) by binding. Indeed, p21(CIP1/WAF1) binds with its cyclin/cdk-binding N terminus to the transforming N-terminal and CR1 region of the E1A proteins. Together, these results lend support to the model that E1A can interfere directly with p21(CIP1/WAF1) function and thereby stimulates cell growth.  (+info)

Early region 1 transforming functions are dispensable for mammary tumorigenesis by human adenovirus type 9. (8/2914)

Some human adenoviruses are tumorigenic in rodents. Subgroup A and B human adenoviruses generally induce sarcomas in both male and female animals, and the gene products encoded within viral early region 1 (E1 region) are both necessary and sufficient for this tumorigenicity. In contrast, subgroup D human adenovirus type 9 (Ad9) induces estrogen-dependent mammary tumors in female rats and requires the E4 region-encoded ORF1 oncoprotein for its tumorigenicity. Considering the established importance of the viral E1 region for tumorigenesis by adenoviruses, we investigated whether this viral transcription unit is also necessary for Ad9 to generate mammary tumors. The nucleotide sequence of the Ad9 E1 region indicated that the gene organization and predicted E1A and E1B polypeptides of Ad9 are closely related to those of other human adenovirus E1 regions. In addition, an Ad9 E1 region plasmid demonstrated focus-forming activity in both low-passage-number and established rat embryo fibroblasts, whereas a large deletion within either the E1A or E1B gene of this plasmid diminished transforming activity. Surprisingly, we found that introducing the same transformation-inactivating E1A and E1B deletions into Ad9 results in mutant viruses that retain the ability to elicit mammary tumors in rats. These results are novel in showing that Ad9 represents a unique oncogenic adenovirus in which the E4 region, rather than the E1 region, encodes the major oncogenic determinant in the rat.  (+info)

Transarterial chemoembolization (TACE) is currently one of the mainstays of palliative treatments worldwide for patients with unresectable Hepatocellular Carcinoma(HCC).However, the long term outcomes were generally poor for HCC patients treated with TACE. Recombinant Human Adenovirus Type 5, an E1B gene deleted adenovirus, is known to have a significant antitumor activity. In addition, local injection of recombinant human adenovirus type 5 can enhance the effect of antitumor therapies (chemotherapy and radiotherapy). The hypothesis is that patients with unresectable HCC may benefit from recombinant human adenovirus type 5 in combination with TACE ...
Summary Evidence is presented here which indicates that the adenovirus DNA-binding protein (DBP) is phosphorylated at a tyrosine residue early in infection. This was suggested by the discovery that a proportion of the label in 32P-labelled DBP was resistant to alkali, and was substantiated by acid hydrolysis of DBP immunoprecipitates and by immunoblotting with a monoclonal antibody against phosphotyrosine. Treatment of [35S] methionine-labelled DBPs with chymotrypsin produced fragments of apparent M r 45K and 39K whereas digestion of 32P-labelled DBP resulted in fragments of 45K and 26K. Consideration of the distribution of 32P label and its alkali stability in these fragments suggested that chymotrypsin cleaved populations of DBP at different sites depending on their phosphorylation states. The conservation, in all of the seven adenovirus serotypes sequenced, of a tyrosine residue (at amino acid 195 in adenovirus type 2) together with its surrounding residues, suggests that phosphorylation
TY - JOUR. T1 - Adenovirus transcription. III. Mapping of viral RNA sequences in cells productively infected by adenovirus type 5. AU - Flint, S. J.. AU - Berget, S. M.. AU - Sharp, Phillip A.. N1 - Funding Information: We thank Jan Haverty for technical assistance and R. J. Roberts for a generous gift of endonuclease Barn HI. S. J. Flint was supported by a fellowship from the Science Research Council of Great Britain, and S. M. Berget by a fellowship from N.I.H. P. A. Sharp is supported by an American Cancer Society faculty grant. This work was funded by grants from the National Cancer Institute (No. CA 13106-03) and the American Cancer Society (No. VC-151).. PY - 1976/7/15. Y1 - 1976/7/15. N2 - Separated strands of restriction endonuclease Eco R1 and Hpa 1 fragments of 32P-labeled Adenovirus 5 DNA have been used in saturation hybridization experiments with cytoplasmic RNA extracted from human cells infected with Adenovirus type 5. The results of such experiments have allowed the construction ...
Human adenoviruses are non-enveloped dsDNA viruses of almost 35 kb in size [1]. HAdV can infect a variety of tissues and cause a wide range of complications like gastroenteritis, hepatitis, myocarditis, keratoconjunctivitis and pneumonia [2, 3]. It is contagion in nature which occurs through direct contact or fomites and virus is also resistant to various physical and chemical agents. Children younger than the age of 5 years and immune compromised persons especially the pediatric patients are most susceptible to these viruses. Worldwide 5-7% respiratory tract infections are ascribed by HAdV in pediatric patients [4] and persons of all ages are susceptible to infections caused by these viruses [5].. Seven known Human adenoviruses species from HAdV-A to HAdV-G are constitute of the genus Mastadenovirus in which all the human adenoviruses are categorized and further divided into different strains [6]. Now 67 types of HAdV have been reported [7]. Their number is rapidly increasing due to ...
Fingerprint Dive into the research topics of Accumulation of early and intermediate mRNA species during subgroup C adenovirus productive infections. Together they form a unique fingerprint. ...
In 2005, a human adenovirus strain (formerly known as HAdV-D22/H8 but renamed here HAdV-D53) was isolated from an outbreak of epidemic keratoconjunctititis (EKC), a disease that is usually caused by HAdV-D8, -D19, or -D37, not HAdV-D22. To date, a complete change of tropism compared to the prototype has never been observed, although apparent recombinant strains of other viruses from species Human adenovirus D (HAdV-D) have been described. The complete genome of HAdV-D53 was sequenced to elucidate recombination events that lead to the emergence of a viable and highly virulent virus with a modified tropism. Bioinformatic and phylogenetic analyses of this genome demonstrate that this adenovirus is a recombinant of HAdV-D8 (including the fiber gene encoding the primary cellular receptor binding site), HAdV-D22, (the ε determinant of the hexon gene), HAdV-D37 (including the penton base gene encoding the secondary cellular receptor binding site), and at least one unknown or unsequenced HAdV-D strain.
In 2005, a human adenovirus strain (formerly known as HAdV-D22/H8 but renamed here HAdV-D53) was isolated from an outbreak of epidemic keratoconjunctititis (EKC), a disease that is usually caused by HAdV-D8, -D19, or -D37, not HAdV-D22. To date, a complete change of tropism compared to the prototype has never been observed, although apparent recombinant strains of other viruses from species Human adenovirus D (HAdV-D) have been described. The complete genome of HAdV-D53 was sequenced to elucidate recombination events that lead to the emergence of a viable and highly virulent virus with a modified tropism. Bioinformatic and phylogenetic analyses of this genome demonstrate that this adenovirus is a recombinant of HAdV-D8 (including the fiber gene encoding the primary cellular receptor binding site), HAdV-D22, (the ε determinant of the hexon gene), HAdV-D37 (including the penton base gene encoding the secondary cellular receptor binding site), and at least one unknown or unsequenced HAdV-D strain.
Entry of adenoviruses into the host cell involves two sets of interactions between the virus and the host cell. Most of the action occurs at the vertices. Entry into the host cell is initiated by the knob domain of the fiber protein binding to the cell receptor. The two currently established receptors are: CD46 for the group B human adenovirus serotypes and the coxsackievirus adenovirus receptor (CAR) for all other serotypes. There are some reports suggesting MHC molecules and sialic acid residues functioning in this capacity as well. This is followed by a secondary interaction, where a motif in the penton base protein interacts with an integrin molecule. It is the co-receptor interaction that stimulates entry of the adenovirus. This co-receptor molecule is αv integrin. Binding to αv integrin results in endocytosis of the virus particle via clathrin-coated pits. Attachment to αv integrin stimulates cell signaling and thus induces actin polymerization resulting in entry of the virion into the ...
Background Human Adenoviruses (HAdVs) cause a wide array of illnesses in all age groups. They particularly cause frequent morbidity among children. In China, human adenovirus types 3, 4, 7, 11, 14, 21, and 55 have caused at least seven outbreaks since 2000. However, limited st...
Adenovirus genomes are linear, non-segmented double-stranded (ds) DNA molecules that are typically 26-46 Kbp long, containing 23-46 protein-coding genes. The example used for the following description is Human adenovirus E, a mastadenovirus with a 36 Kbp genome containing 38 protein-coding genes. While the precise number and identity of genes varies among adenoviruses, the basic principles of genome organization and the functions of most of the genes described in this article are shared among all adenoviruses. The 38 genes in the Human Adenovirus E genome are organized in 17 transcription units, each containing 1-8 coding sequences. Alternative splicing during processing of the pre-mRNAs produced by each transcription unit enable multiple different mRNAs to be produced from one transcription unit. The E1A, E1B, E2A, E2B, E3, and E4 transcription units are successively transcribed early in the viral reproductive cycle. The proteins coded for by genes within these transcription units are mostly ...
Vectors derived from human adenovirus type 5, which typically lack the E1A and E1B genes, induce robust innate immune responses that limit their therapeutic efficacy. mutant-infected cells in the absence or presence of exogenous IFN. Both the concentration of viral genomes detected during the late phase and the numbers of viral replication centers formed were strongly reduced in IFN-treated cells in the absence of the E1B protein, despite production of similar quantities of viral replication proteins. These defects could not be attributed to degradation of entering viral genomes, induction of apoptosis, or failure to reorganize components of PML nuclear bodies. Nor was assembly of Nutlin 3a the E1B- and E4 Orf6 protein- E3 ubiquitin ligase required to prevent inhibition of viral replication by IFN. However, by using RT-PCR, the E1B 55 kDa protein was demonstrated to be a potent repressor of expression of IFN-inducible genes in IFN-treated cells. We propose that a primary function of the ...
To define the bottlenecks that restrict antigen expression after oral administration of viral-vectored vaccines, we tracked vectors derived from the human adenovirus type 5 at whole body, tissue and cellular scales throughout the digestive tract in a murine model of oral delivery. After intragastric administration of vectors encoding firefly luciferase or a model antigen, detectable levels of transgene-encoded protein or mRNA were confined to the intestine, and restricted to delimited anatomical zones. Expression of luciferase in the form of multiple small bioluminescent foci in the distal ileum, cecum and proximal colon suggested multiple crossing points. Many foci were unassociated with visible Peyers patches, implying that transduced cells lay in proximity to villous rather than follicle-associated epithelium, as supported by detection of transgene-encoded antigen in villous epithelial cells. Transgene-encoded mRNA but not protein was readily detected in Peyers patches, suggesting that post
Human adenovirus (HAdV) is a common pathogen causing a broad spectrum of diseases. HAdV encodes the pVII protein, which is involved in nuclear delivery, protection and expression of viral DNA. To suppress the cellular interferon (IFN) and RNA interference (RNAi) systems, HAdVs encode non-coding virus-associated (VA) RNAs. In this thesis we have investigated the functional significance of the pVII protein and VA RNAI in HAdV-5 infected cells.. We report that the propeptide module is the destabilizing element targeting the precursor pVII protein for proteasomal degradation. We also found that the Cul3-based E3 ubiquitin ligase complex alter the precursor pVII protein stability via binding to the propeptide sequence. In addition, we show that inhibition of the Cul3 protein reduces HAdV-5 E1A gene expression. Collectively, our results suggest a novel function for the pVII propeptide module and involvement of Cul3 in viral E1A gene expression.. Our studies show that the cellular E3 ubiquitin ligase ...
Human adenovirus 12 ATCC ® VR-863D™ Designation: DNA from Human adenovirus 12 strain Huie [ATCC ® VR-863™] Application: It is suitable for use in polymerase chain reaction (PCR) for viral gene products and other molecular virology applications. Respiratory research
Human adenovirus 12 ATCC ® VR-863D™ Designation: DNA from Human adenovirus 12 strain Huie [ATCC ® VR-863™] Application: It is suitable for use in polymerase chain reaction (PCR) for viral gene products and other molecular virology applications. Respiratory research
It has been known for some time that the human adenovirus serotype 5 (Ad5) E4orf6 and Sox18 E1B55K proteins work in concert to degrade p53 and to regulate selective export of late viral mRNAs during productive infection. H1299 human lung carcinoma cells after expression of E1B55K and E4orf6 using adenovirus vectors. Several species were detected and identified by mass spectroscopy and for one of these integrin α3 we went on in a parallel study to confirm it as a substrate of the complex (F. Dallaire et al. J. Virol. 83:5329-5338 2009 Although the system has some limitations it may still be of some general use in identifying candidate substrates of any viral cullin-based E3 ubiquitin ligase complex and we suggest a series of criteria for substrate validation. During the past decade protein degradation has become increasingly recognized SVT-40776 (Tarafenacin) as a critical mechanism by which cells regulate a number of fundamental processes (reviewed in references 37 57 and 59). Degradation ...
Adenoviral nucleic acid was detected by polymerase chain reaction (PCR) in pharyngeal and rectal swab samples of a cat seropositive for adenovirus and suffering from transient hepatic failure. The samples were taken at a one-year interval, and both faecal samples as well as the second pharyngeal sample were positive in ... read more PCR performed with general adenovirus primers. The size of the amplified products corresponded to that of the positive control. The identity of the amplicons was also confirmed by DNA sequencing. The 301 bp long hexon gene fragment was very similar to but distinguishable from the corresponding hexon sequence of human adenovirus type 2. This result suggests the possibility of persistent carrier status and shedding of adenovirus in cats. show less ...
Antisera against hexons of serotypes 2, 4, 5, and 6 (subgroup III), and 15 (subgroup II) were absorbed with purified hexons of various serotypes representing the different subgroups of human adenoviruses. Group, subgroup, and type specificities of hexons could be distinguished. The subgroup specificity of type 4 hexons resembled that of hexons of subgroup I members (types 3, 11, and 16). Antihexon sera gave a type-specific inhibition of virion-associated hemagglutinin. The inhibiting activity of different sera was found to be inversely related to the length of fibers of the serotype concerned. Virions of serotypes carrying fibers shorter than about 20 nm (types 3, 4, 9, 11, and 15) were readily inhibited, whereas those of serotypes with longer fibers (types 2 and 6) were inhibited only by relatively large amounts of antibody measured in terms of homotypic complement fixation activity. The reciprocal cross-neutralization between serotypes 4 and 16 was studied separately. Hexons of both serotypes ...
The precursor of the 55K adenovirus terminal protein is an 87K protein that is covalently linked to viral DNA. This protein is likely to be identical to the 80,000 dalton protein described by Challberg et al. (1980). The mRNA for the 87K terminal protein precursor, like that for the E2-72K DNA binding protein, is detectable at both early and late times of infection, and its production is sensitive to protein synthesis inhibition (Lewis and Mathews, 1980). The 87K protein, together with proteins of 105,000 and 75,000 daltons, are translated from leftward transcribed (1-strand) messenger RNAs that are complementary to the viral genome between positions 11.2 and 31.5. Additional hybridization to the region between coordinates 37.3 and 41 suggests that the RNA body is spliced to sequences mapping farther right in the genome. Electron microscopic heteroduplex analysis has revealed a family of 1-strand RNAs that probably encode these proteins. The RNA bodies extend from coordinated 30, 26 and 23 to ...
Recombinant adenoviruses currently are used for a variety of purposes, including gene transfer in vitro, vaccination in vivo, and gene therapy (1-4). Several features of adenovirus biology have made such viruses the vectors of choice for certain of these applications. For example, adenoviruses transfer genes to a broad spectrum of cell types, and gene transfer is not dependent on active cell division. Additionally, high titers of viruses and high levels of transgene expression generally can be obtained.. Decades of study of adenovirus biology have resulted in a detailed picture of the viral life cycle and the functions of the majority of viral proteins (5, 6). The genome of the most commonly used human adenovirus (serotype 5) consists of a linear, 36-kb, double-stranded DNA molecule. Both strands are transcribed and nearly all transcripts are heavily spliced. Viral transcription units are conventionally referred to as early (E1, E2, E3, and E4) and late, depending on their temporal expression ...
The cryo-electron microscopy (cryo-EM) structure of the complex between the trimeric human adenovirus B serotype 3 fibre knob and human desmoglein 2 fragments containing cadherin domains EC2 and EC3 has been published, showing 3:1 and 3:2 complexes. Here, the crystal structure determined at 4.5 Å resolution is presented with one EC2-EC3 desmoglein fragment bound per fibre knob monomer in...
Adenovirus has been associated with both sporadic and epidemic disease and, with regard to infections among military recruits, who were routinely immunized against types 4 and 7 from 1971 until the cessation of vaccine production in 1996. Adenovirus became a significant cause of economic cost and morbidity in this setting. A live oral vaccine against adenovirus types 4 and 7 was approved for use in this population by the US Food and Drug Administration (FDA) in 2011, and subsequent incidence of acute respiratory disease declined.. Of interest is the role of adenoviruses as vectors in vaccination and in gene therapy. [1, 2, 3] Adenoviruses can infect various cells, both proliferating and quiescent, and thus hold the promise of targeting many different tissues and diseased cell lines.. The genome of adenovirus is well known and can be modified with relative ease to induce lysis or cytotoxicity of a specified cell line without affecting others.. The virus itself can be engineered to remove its ...
The E4 region of human adenovirus type 2 is predicted to encode seven proteins as judged from its nucleotide sequence and the pattern of differential splicing of its transcript. Two of the open reading frames (ORFs), ORF1 and ORF2, had been identified as being disrupted in the recently published sequence of the related serotype 5 virus. These ORFs were resequenced and found to be intact in the wt300 strain of adenovirus type 5.
Most adenoviruses bind directly to the coxsackie and adenovirus receptor (CAR) on target cells in vitro, but recent research has shown that adenoviruses can also use soluble components in body fluids for indirect binding to target cells. These mechanisms have been identified upon addressing the questions of how to de- and retarget adenovirus-based vectors for human gene and cancer therapy, but the newly identified mechanisms also suggest that the role of body fluids and their components may also be of importance for natural, primary infections. Here we demonstrate that plasma, saliva, and tear fluid promote binding and infection of adenovirus type 5 (Ad5) in respiratory and ocular epithelial cells, which corresponds to the natural tropism of most adenoviruses, and that plasma promotes infection by Ad31. By using a set of binding and infection experiments, we also found that Ad5 and Ad31 require coagulation factors IX (FIX) or X (FX) or just FIX, respectively, for efficient binding and infection. ...
Your basket is currently empty. i ,p>When browsing through different UniProt proteins, you can use the basket to save them, so that you can back to find or analyse them later.,p>,a href=/help/basket target=_top>More...,/a>,/p> ...
NEW YORK (GenomeWeb News) - Researchers from China and the US will sequence 100 human adenoviruses, including ones that cause respiratory, gastrointestinal, and ocular diseases, under a partnership announced today.
The quest for an efficacious HIV vaccine has resulted in several clinical trial failures, including the Step Trial, which used a replication-incompetent adenovirus (AdV) vector called human adenovirus type 5 (HAdV-5). Despite eliciting strong cellular immune responses, these trials were prematurely halted due to statistical futility resulting from increased HIV acquisition in vaccinated individuals. The Step Study showed increased HIV susceptibility in HAdV-5 baseline seropositive subjects, which complicates the use of HAdV-5 vectors since pre-existing neutralizing antibodies (nAb) to HAdV-5 are prevalent worldwide. Sampling the unique immunological phenotypes of the rectal mucosa - the site of HIV infection in the Step Study, and of AdV persistence and trafficking - could help explain this trial, since only peripheral blood (PBMC) was collected from subjects. We obtained rectal lamina propria T lymphocytes (rLPL) from a rhesus macaque (RM) model vaccinated with a species-specific simian Ad type 7 (SAdV
TY - JOUR. T1 - Repression of cytochrome P‐450c gene expression by cotransfection with adenovirus E1a DNA. AU - SOGAWA, Kazuhiro. AU - HANDA, Hiroshi. AU - FUJISAWA‐SEHARA, Atsuko. AU - HIROMASA, Takako. AU - YAMANE, Miyuki. AU - FUJII‐KURIYAMA, Yoshiaki. PY - 1989/5. Y1 - 1989/5. N2 - Gene expression of rat cytochrome P‐450c (P‐450c) depends upon inducible enhancers scattered in the 5′‐upstream region of the gene. We show that expression of the P‐450c gene is repressed by contransfection with adenovirus E1a DNA, regardless of the presence or absence of inducers, in a transient expression system of HeLa cells. Since cotransfection of either 13S or 12S E1a cDNA was effective in the repression, the region necessary for repression could be separated from that of transactivation of other adenovirus early genes. Moreover, we investigated the regions responsible for the inhibitory activity using in‐frame deletion mutants lacking internal or external portions of the E1a proteins. The ...
Full custom adenovirus production services, adenovirus vector construction, adenovirus packaging, adenovirus amplification, adenovirus purification, adenovirus titering.
What is the definition of ADENOVIRUS? What is the meaning of ADENOVIRUS? How do you use ADENOVIRUS in a sentence? What are synonyms for ADENOVIRUS?
SignaGen Laboratories, A Gene Delivery Company Providing Custom AAV Adenovirus Lentivirus Production Services & Manufacturing DNA/siRNA Transfection Reagents... Ad-GIT2 [SL100903] - Product Category: Human Adenovirus Type5 (dE1/E3) expressing G Protein-coupled Receptor Kinase Interactor 2 (GIT2) under a CMV promoter. Product Information Product Name: G Protein-coupled Receptor Kinase Interactor 2, pre-packaged adenovirus, ready to ship and ready to use format. Promoter: CMV Titer: 1E+10~1E+11 PFU/ml Storage Buffer: DMEM with 2.5% BSA, 2.5% glycerol Gene Information Gene Name: G
SignaGen Laboratories, A Gene Delivery Company Providing Custom AAV Adenovirus Lentivirus Production Services & Manufacturing DNA/siRNA Transfection Reagents... Ad-PRKD3 [SL100971] - Product Category: Human Adenovirus Type5 (dE1/E3) expressing Protein Kinase D3 (PRKD3). Product Information Product Name: Protein Kinase D3, pre-made adenovirus, ready to ship and ready to use format. Promoter: CMV Titer: 1E+10 ~ 1E+11 PFU/ml Storage Buffer: DMEM with 2.5% BSA, 2.5% glycerol Gene Information Gene Name: Protein Kinase D3 NCBI Acc. #: NM_005813 Gene Symbol: PRKD3 Gene ID:
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
There are many stages in the development of a new drug for viral infection and such processes are even further complicated for adenovirus by the fact that there are at least 51 serotypes, forming six distinct groups (A-F), with different degree of infectivity. This review attempts to address the importance of developing pharmaceuticals for adenovirus and also review recent development in drug discovery for adenovirus, including newer strategies such as microRNA approaches. Different drug screening strategies will also be discussed.
Adenovirus Type 9, 0.1 mg. The many different serotypes of human adenoviruses (Ad) are divided into six subgroups, of which all Ad subgroup A and B and two subgroup D Ads can elicit tumors in infected rodents.
A HIV-1 DNA prime-recombinant Adenovirus Type 5 (rAd5) increase vaccine failed to guard against HIV-1 acquisition. is certainly towards the gp41 subunit from the envelope (Env) glycoprotein from the pathogen (1). This antibody response derives from polyreactive B cells that cross-react with Env and intestinal microbiota (IM) (2, 3). Nevertheless, it is unidentified if an identical gp41-reactive Ab response would take place in the placing of HIV-1 Env vaccination. A DNA leading, recombinant adenovirus serotype 5 (rAd5) increase vaccine that included HIV and genes, and a trivalent combination of clade A, B and C gp140 genes formulated with both gp120 and gp41 elements was examined in the HIV Vaccine Studies Network (HVTN) [stage Ib (HVTN 082), GW-786034 stage II (HVTN 204), stage IIb (HVTN 505) efficiency trial] and various other clinical studies [stage I/II (RV172), stage I (V001)] (4C7). This vaccine was the GW-786034 initial vaccine formulated with the ectodomain from the Env gp41 component, ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
In tumor cells, the p53 pathway is often disrupted. Therefore, recovering the function of wild-type p53 and its targets in tumor cells is a key therapeutic objective. In head and neck cancer, p53 mutations are frequent, and the incidence of p53 mutations increases with progression of head and neck cancer (46, 47). Therefore, a recombinant human adenovirus that expresses functional wild-type p53 has been approved by the Chinese government for the treatment of head and neck carcinoma (48-50). Treatments showed that antitumor efficacy was associated with the expression and activity of functional p53, and adverse effects were also significant (51-54). Recently, pharmacologically activated wild-type p53 by small-molecule compound RITA is reported to inhibit glycolytic enzymes and, therefore, induce robust apoptosis in cancer cells (55). In addition, enhancement of p53 protein stability is also a target in restoring wild-type p53 activity in cancer cells. The protein level of wild-type p53 is ...
Mouse anti Adenovirus Hexon antibody, clone 7C11 reacts with human, canine, bovine, monkey and rat adenoviruses. It is very likely that it
13:2; potential epub Adenovirus notions; tenure bhakti 1? On the epub Adenovirus Methods and of this interest, are above under form. 1282 An epub Adenovirus Methods and Protocols: Adenoviruses, Ad study read by Porten - Szubin 1987:187.
购买Adenovirus Type 5兔多克隆抗体(ab6982),Adenovirus Type 5抗体经WB验证,62篇文献引用,9个独立用户反馈。产品出库一年都在质保范围内。中国现货速达。
Greetings- Im interested in the net surface charge on mammalian viruses and how viral charge affects viral adsorption. It is my understanding (correct me if Im wrong) that mammalian viruses have a net negative charge at physiologic pH. I find this interesting because of the net negative charge on mammalian cells due to their phospholipid content. I have been working with human adenovirus gene transfer vectors and have found that the pI of adeno capsid proteins are: Hexon = 4.5 and penton = 4.7. pI of fiber protein on adenovirus is reported to be 7.07. From these data, I have concluded that, at pH 7.0, an adeno virus particle has a negative core (Hexon and penton) with positively charged fiber protrusions. Is this correct (my chemistry is a little rusty)? If so, it would explain how negatively charged adenovirus particles might interact with negatively charged cell surfaces (via positive fiber protein). Thank you, in advance for any comments and corrections. In reading this note, if any related ...
1NLN: Crystallographic structure at 1.6-A resolution of the human adenovirus proteinase in a covalent complex with its 11-amino-acid peptide cofactor: insights on a new fold
We identified previously unrecognized variations in the fiber gene and E4 ORF 6/7 peptides among the various genome types of Ad7. In particular, multiple amino acid changes at E4 ORF 6/7 peptides showed genome type-specific differences between Ad7d and a novel genome type Ad7l. However, no genetic divergence was identified in the hexon gene or in E3 peptides among the 12 Korean strains of Ad7. This result indicates that the nucleotide structures of the fiber gene and of E4 ORF 6/7 peptides might have contributed to the genetic heterogeneity during the Ad7 epidemics in Korea, whereas those of the hexon gene and E3 were highly conserved.. So far, the fiber gene and E4 have rarely been addressed with regard to the evolution and molecular epidemiology of Ad7 strains (17). Restriction analysis of genomic DNA has been the most popular method of describing the molecular epidemiology of the adenoviruses (18). However, little is known about sequence variations with regard to the genome type within an ...
The adenovirus E1B gene products are required for productive infection of human cells and for complete transformation of rodent cells in cooperation with the E1A gene products. Two major, unrelated polypeptides of 55,000 (55K) and 19,000 (19K) daltons are encoded by the E1B region. The 55K protein is required for efficient DNA replication, late mRNA transport to the cytoplasm and shut-off of cellular mRNA transport in productively infected cells. This protein is required for virus-mediated, but not DNA-mediated, transformation of rodent cells. It appears that the 55K protein does not directly contribute to cell transformation, but influences the oncogenicity of adenoviruses when they are inoculated into newborn hamsters. In contrast, the 19K protein is required for adenovirus induced cellular transformation and oncogenicity and localizes to membranes of the nuclear envelope, cytoplasm and the cell surface in transformed cells. This protein affects the efficiency of virus growth in some, but not ...
Looking for online definition of adenovirus early region genes in the Medical Dictionary? adenovirus early region genes explanation free. What is adenovirus early region genes? Meaning of adenovirus early region genes medical term. What does adenovirus early region genes mean?
RIGOTTO, C et al. Evaluation of HA negatively charged membranes in the recovery of human adenoviruses and hepatitis A virus in different water matrices. Mem. Inst. Oswaldo Cruz [online]. 2009, vol.104, n.7, pp.970-974. ISSN 0074-0276. http://dx.doi.org/10.1590/S0074-02762009000700005.. Human adenoviruses (HAdV) and hepatitis A virus (HAV) are shed in the faeces and consequently may be present in environmental waters, resulting in an increase in pathogen concentration that can affect water quality and human health. The aim of this study was to evaluate an adsorption-elution method which utilizes negatively charged membrane HA to determine the efficient recovery of HAdV and HAV from different water matrices and to combine this procedure with a qualitative molecular method (nested RT-PCR and nested PCR). The best efficiency recovery was achieved in distilled water and treated wastewater effluent (100%) for both viruses and in recreational lagoon water for HAV (100%). The efficiency recovery was 10% ...
Human adenovirus, of the Adenoviridae family, is a nonenveloped icosahedral particle containing a single linear dsDNA genome. Human adenovirus comprises 6 species (A through F) consisting of serotypes 1 - 51, and associated with a variety of clinical illnesses. Serotypes 1-39, 42-51 are associated with a variety of respiratory disease, generally in children and immunocompromised persons; whereas serotypes 40 and 41 is associated with enteric disease (enteric adenovirus) particularly in children - these enteric adenoviruses will not be detected with the assay described here. Serotypes 12, 18, 31 have a high oncogenic potential and serotypes 4 and 7 are associated with acute respiratory disease (ARD) frequently in military recruits and typically occur in the winter and spring. Smaller outbreaks of serotypes 3, 4, and 7 occur in the summertime and are associated with contaminated swimming pool water.. Adenovirus infections of the eye may lead to pharyngo-conjunctival fever, follicular ...
Human adenoviruses (HAdVs) cause a wide range of diseases worldwide, including respiratory infections. Studies on HAdV molecular epidemiology are limited in Cameroon. The purpose of this study is to document the different types HAdV circulating in Cameroon in children with acute respiratory infections. Nasopharyngeal swabs were collected from 811 children under 15 years from 2011 to 2014. The HAdV detection was assessed by semi-quantitative generic PCR r-gene®. The HAdV-positive samples were typed by amplification and sequencing of partial hexon gene and a real-time PCR. Demographic data were collected and analyzed. The infection and hospitalization risk factors were assessed thought the Chi-square test. A total of 137/220 HAdV-positive samples were amplified successfully. Six species of HAdV (Mastadenovirus A to F) were detected with B (108/220) and C (47/220) being the predominant strains. Hospitalization and age were significantly associated to HAdV-B and HAdV-C respectively. Phylogenetic analysis
Adenovirus type 37 (Ad37) is a leading cause of epidemic keratoconjunctivitis (EKC), a severe and highly contagious ocular disease. Whereas most other adenoviruses infect cells by engaging CD46 or the coxsackie and adenovirus receptor (CAR), Ad37 binds previously unknown sialic acid-containing cell surface molecules. By glycan array screening, we show here that the receptor-recognizing knob domain of the Ad37 fiber protein specifically binds a branched hexasaccharide that is present in the GD1a ganglioside and that features two terminal sialic acids. Soluble GD1a glycan and GD1a-binding antibodies efficiently prevented Ad37 virions from binding and infecting corneal cells. Unexpectedly, the receptor is constituted by one or more glycoproteins containing the GD1a glycan motif rather than the ganglioside itself, as shown by binding, infection and flow cytometry experiments. Molecular modeling, nuclear magnetic resonance and X-ray crystallography reveal that the two terminal sialic acids dock into two of
RNA molecules from nuclear and cytoplasmic polyribosomes of adenovirus-infected HeLa cells were compared by hybridization to analyse the sequence content. Nuclear polyribosomes were released by exposure of intact detergent-washed nuclei to poly(U) and purified. Cytoplasmic polyribosomes were also purified from the same cells. To show that nuclear polyribosomes contain ribosomes linked by mRNA, polyribosomes were labelled with methionine and uridine in the presence of actinomycin D in adenovirus-infected cells. Purified nuclear polyribosomes were treated with EDTA under conditions which dissociate polyribosomes into ribosomes and subunits with a simultaneous release of mRNA, and sedimented. The treatment dissociated these polyribosomes, releasing the mRNA from them. Radiolabelled total RNA from each polyribosome population was fractionated in sucrose gradients into several pools or hybridized to intact adenovirus DNA to select virus-specific RNA. Sucrose-gradient-fractionated pool-3 RNA (about ...
The utility of recombinant adenovirus serotype 5 (rAd5) vector-based vaccines for HIV-1 and other pathogens will likely be limited by the high prevalence of pre-existing Ad5-specific neutralizing Abs (NAbs) in human populations. However, the immunodominant targets of Ad5-specific NAbs in humans remain poorly characterized. In this study, we assess the titers and primary determinants of Ad5-specific NAbs in individuals from both the United States and the developing world. Importantly, median Ad5-specific NAb titers were ,10-fold higher in sub-Saharan Africa compared with the United States. Moreover, hexon-specific NAb titers were 4- to 10-fold higher than fiber-specific NAb titers in these cohorts by virus neutralization assays using capsid chimeric viruses. We next performed adoptive transfer studies in mice to evaluate the functional capacity of hexon- and fiber-specific NAbs to suppress the immunogenicity of a prototype rAd5-Env vaccine. Hexon-specific NAbs were remarkably efficient at ...
The high prevalence of pre-existing anti-Ad5 immunity in human populations may substantially limit the immunogenicity and clinical utility of rAd5 vector-based vaccines for HIV-1 and other pathogens. Our studies demonstrate ,90% Ad5 seroprevalence in sub-Saharan Africa with median NAb titers ,10-fold higher than those found in the United States. These data suggest that rAd5 vectors should be engineered to evade dominant Ad5-specific NAbs before their use as vaccine vectors in the developing world. To determine the principal targets of Ad5-specific NAbs, we exploited the lack of detectable serologic cross-reactivity between Ad5 and Ad35 (8). Virus neutralization studies using capsid chimeric rAd5/rAd35 vectors and serum samples from both humans and mice demonstrated that Ad5-specific NAbs were directed primarily against the Ad5 hexon protein. Fiber-specific NAbs were detected at low frequencies in vitro but were substantially less efficient than hexon-specific NAbs at blunting rAd5 vaccine ...
Last year, Chiu and colleagues also identified another new adenovirus, named simian adenovirus C, which sickened four of nine captive baboons and killed two of them at a primate facility in 1997. Several staff members at the facility also complained of upper respiratory symptoms at the time of the outbreak. Re-examining the samples many years later, Chiu and his colleagues found antibodies targeted to simian adenovirus C in the human samples.. Chiu concluded that staff members had been exposed to the new virus, and that the virus may have jumped from baboon to human, an idea also supported by follow-up experiments in which laboratory strains of simian adenovirus C efficiently infected both human and baboon cells.. Adenoviruses to date have not generally been linked to cross-species infections between monkeys and humans, Chiu said.. In light of these findings, however, he said the normal vigilance in tracking animal viruses that might also infect humans should extend beyond influenza and ...
Seventy-eight cases were identified in patients from four eye care practices, two family practices, and the hospital emergency department. The median patient age was 45 years (range = 9 months-90 years), and 33 (42%) were men. Ocular signs and symptoms included redness (68%), watery discharge (50%), and pain (29%). Severe signs included corneal infiltrates (17%) and pseudomembranes (6%). At least 12 cases (15%) were health care-associated (Figure). One health care-associated case occurred in a health care worker. Seventeen patients whose infections were not health care-associated reported a symptomatic household or community contact. Among 45 conjunctival swabs available for testing, 19 (42%) were positive for HAdV-8. Genome sequences obtained from five HAdV-8 isolates were 100% identical with one another and showed 97.7% (accession number AB861610.1) to 99.9% (accession number KT340070.1) nucleotide sequence similarity to other HAdV-8 genome sequences available in GenBank, the National ...
Mature human adenovirus particles contain four minor capsid proteins, in addition to the three major capsid proteins (penton base, hexon and fiber) and several proteins associated with the genomic core of the virion. Of the minor capsid proteins, VI plays several crucial roles in the infection cycle of the virus, ... read more including hexon nuclear targeting during assembly, activation of the adenovirus proteinase (AVP) during maturation and endosome escape following cell entry. VI is translated as a precursor (pVI) that is cleaved at both N- and C-termini by AVP. Whereas the role of the C-terminal fragment of pVI, pVIc, is well established as an important co-factor of AVP, the role of the N-terminal fragment, pVIn, is currently elusive. In fact, the fate of pVIn following proteolytic cleavage is completely unknown. Here, we use a combination of proteomics-based peptide identification, native mass spectrometry and hydrogen-deuterium exchange mass spectrometry to show that pVIn is associated ...
Subgroup C adenoviruses, including serotypes 2 and 5, from which most therapeutic adenoviruses are derived, rely on CAR as the primary binding site on the host cell. This receptor has been shown to be crucial for sufficient virus uptake (22) . In cancer cells, however, CAR expression is frequently lost, especially in highly malignant cancer cell lines, leading to a significant decrease in adenovirus uptake (5 , 6) . Our own observations are in agreement with these reports: we frequently found reduced CAR expression at the cell surface in high-grade primary liver cancer and metastases of colorectal cancer. 4 This study investigates the molecular mechanisms involved in reducing CAR expression in cancer cells and explores the possibility of pharmacologically manipulating CAR expression levels.. Increasing evidence exists for a potential physiological role of CAR as a cell adhesion molecule. CAR forms homodimers, was found to physically interact with the tight-junction protein ZO-1, and participates ...
Definition of Adenovirus E3 10.4K/14.5kD Protein in the Legal Dictionary - by Free online English dictionary and encyclopedia. What is Adenovirus E3 10.4K/14.5kD Protein? Meaning of Adenovirus E3 10.4K/14.5kD Protein as a legal term. What does Adenovirus E3 10.4K/14.5kD Protein mean in law?
To delineate the function of adenovirus early region 4 (E4) gene products, we constructed a set of mutant viruses which carry defined lesions within this coding region. Deletion and insertion mutations within six of seven known E4 coding regions had no measurable effect on virus growth in cultured cells. A variant carrying a deletion within the last coding region (encoding a 34,000-molecular-weight polypeptide) was modestly defective, and a mutant lacking the majority of the E4 region was severely defective for growth. The phenotypes of the two defective mutants are similar and complex. Both display perturbations in DNA replication, translation of the E2A mRNA, accumulation of late viral mRNAs, and host cell shutoff. ...
86 PCR positive samples were inoculated onto Hep-2 cell for virus isolation, and 23 HAdV strains were isolated through 7-21 days post-inoculation, with isolation rate of 26.74%, which was close to that of 21.43% in the previous study (Thounaojam et al. 2016). The hexon gene sequence has widely been used for the classification of adenovirus types. In this study, the hexon gene of the 23 HAdV isolates was amplified and sequenced using primer designed by Sarantis et al. (Sarantis et al. 2004) (Forward primer: 5′-CTGATGTACTACAACAGCACTGGCAACATGGG-3′, Reverse primer: 5′-GCGTTGCGGTGGTGGTTAAATGGGTTTACGTTGTCCAT-3′, the amplicon length is 580-605 bp), and the nucleotide sequences were submitted to GenBank (accession numbers MN389388-MN389410). Molecular typing assignments were based on the identity of the closest matching sequences after both BLAST and phylogenetic analysis. Results showed that 9 of the 23 HAdV isolates (8 HAdV-3 and 1 HAdV-7) belonged to species B and 12 of the 23 (5 HAdV-1 and 7 ...
The information presented here serves as a brief introduction to adenovirus, recombinant adenovirus, and their advantages over alternative viral vectors.
The 293 cell line was derived from primary cultures of human embryonic kidney (HEK) cells with sheared fragments of adenovirus (Ad) 5 DNA (Graham et al. 1977). HEK 293 cells contain the nucleotides 1-4344 of Ad5 which are located within the pregnancy-specific ß-1-glycoprotein 4 (PSG 4) gene. The transforming region of the human adenovirus contains the early region (E1), comprising two transcription units, E1a and E1b, whose products are essential and sufficient for mammalian cell transformation by adenoviruses (Louis et al. 1997). Because 293 cells express E1 gene products they are extensively used for the production of E1-deleted Ad viruses. Adeno-associated viruses (AAVs) belong to the family of Parvoviridae, being one of the smallest single-stranded and non-enveloped DNA viruses. AAVss are replication-deficient and have required co-infection with a helper adeno- or herpes virus for productive infection. The AAV Helper-free system takes advantage of the identification of the specific ...
Purification of recombinant adenovirus type 3 dodecahedric virus-like particles for biomedical applications using short monolithic columns
TY - JOUR. T1 - Biobased monoliths for adenovirus purification. AU - Fernandes, Cláudia S. M.. AU - Gonçalves, Bianca. AU - Sousa, Marcos F. Q.. AU - Martins, Duarte L.. AU - Barroso, Telma. AU - Pina, Ana Sofia. AU - Peixoto, Cristina. AU - Aguiar-Ricardo, Ana. AU - Roque, A. Cecília A.. N1 - info:eu-repo/grantAgreement/FCT/COMPETE/132972/PT# info:eu-repo/grantAgreement/FCT/3599-PPCDT/118317/PT# info:eu-repo/grantAgreement/FCT/3599-PPCDT/135802/PT# Sem PDF conforme despacho.. PY - 2015/4/1. Y1 - 2015/4/1. N2 - Adenoviruses are important platforms for vaccine development and vectors for gene therapy, increasing the demand for high titers of purified viral preparations. Monoliths are macroporous supports regarded as ideal for the purification of macromolecular complexes, including viral particles. Although common monoliths are based on synthetic polymers as methacrylates, we explored the potential of biopolymers processed by clean technologies to produce monoliths for adenovirus purification. ...
Adenoviruses were identified as unique viruses in 1953 by Rowe and coworkers when virus was isolated from human adenoids. Because adenoviruses infect various cells they are considered an excellent vector delivery system. A major obstacle to efficient infection by recombinant adenoviruses is the humo.... Full description. ...
Human adenoviruses (HAdVs) often cause mild respiratory infections. These infections, however, can potentially become fatal in immunosuppressive patients. Unfortunately, there has been no specific anti-HAdV drug approved for treatment of HAdV infections. In this study, a time-course transcriptome of HAdV-infected human lung epithelial cells (A549 cells) was performed and compared with perturbation datasets of 890 drug-treated A549 cells from the library of integrated network-based cellular signatures (LINCS) database to predict previously unknown therapeutic drug-HAdV relationships using a characteristic direction (CD) algorithm. We performed experiments to validate a prediction for the anti-diabetic drug rosiglitazone as a candidate drug for treatment of anti-HAdV both in vivo and in vitro. The Type I interferon (IFNs) signaling pathway was negatively regulated during the course of HAdV infection and rosiglitazone increased STAT1 phosphorylation for antiviral IFN response induction. Taken ...
Yakimovich, A; Gumpert, H; Burckhardt, C J; Lutschg, V A; Jurgeit, A; Sbalzarini, I F; Greber, U F (2012). Cell-free transmission of human adenovirus by passive mass transfer in cell culture simulated in a computer model. Journal of Virology, 86(18):10123-10137.. Cardinale, J; Paul, G; Sbalzarini, I F (2012). Discrete region competition for unknown numbers of connected regions. IEEE transactions on image processing : a publication of the IEEE Signal Processing Society, 21(8):3531-3545.. Helmuth, J A; Burckhardt, C J; Greber, U F; Sbalzarini, I F (2009). Shape reconstruction of subcellular structures from live cell fluorescence microscopy images. Journal of Structural Biology, 167(1):1-10.. Helmuth, J A; Burckhardt, C J; Koumoutsakos, P; Greber, U F; Sbalzarini, I F (2007). A novel supervised trajectory segmentation algorithm identifies distinct types of human adenovirus motion in host cells. Journal of Structural Biology, 159(3):347-358.. ...
Abstract Background Viruses bind to specific cellular receptors in order to infect their hosts. The specific receptors a virus uses are important factors in determining host range, cellular tropism, and pathogenesis. For adenovirus, the existing model of entry requires two receptor interactions. First, the viral fiber protein binds Coxsackie and Adenovirus Receptor (CAR), its primary cellular receptor, which docks the virus to the cell surface. Next, viral penton base engages cellular integrins, coreceptors thought to be required exclusively for internalization and not contributing to binding. However, a number of studies reporting data which conflicts with this simple model have been published. These observations have led us to question the proposed two-step model for adenovirus infection. Results In this study we report that cells which express little to no CAR can be efficiently transduced by adenovirus. Using competition experiments between whole virus and soluble viral fiber protein or ...
ViroTag® ADVX (for manual sampling) utilizes a fluorescently-labeled, high-affinity antibody which binds to a unique epitope specifically expressed on adenovirus. The product has been shown to quantify multiple serotypes (2-6) and is considered pan-reactive. With the Virus Counter 3100, use this rapid, no-wash labeling procedure and take adenovirus quantification to new levels of accuracy, speed and simplicity!. Product specifications: The ViroTag ADVX kit (catalog number 92097) contains all reagents and consumables necessary to analyze 200 samples using the Virus Counter 3100 instrument for manual sampling, including:. ...
This 2008 Molecular Cancer Therapeutics paper by Yacoub, A., etc. utilizes the following products and services from Vector Biolabs: Akt1 (dn) Adenovirus, CrmA Adenovirus, Caspase 9 (dn) Adenovirus, human BCL-xL shRNA Adenovirus, Baculoviral IAP repeat-containing 4 Adenovirus, CASP8 and FADD-like apoptosis regulator Adenovirus, MEK1 (dn) Adenovirus.
Adenovirus and pregnancy - What is adenovirus infection? Many forms. This virus can produce several forms of infection. Pharyngo-conjunctival fever (or swimming pool fever) is one of the better known, but also gastroenteritis, upper respiratory tract infections and pneumonia can all occur.
Adenovirus Early Proteins: Proteins encoded by adenoviruses that are synthesized prior to, and in the absence of, viral DNA replication. The proteins are involved in both positive and negative regulation of expression in viral and cellular genes, and also affect the stability of viral mRNA. Some are also involved in oncogenic transformation.
Trentin, J J. and Bryan, E, Immunization of hamsters and histoisogenic mice against trans- plantation of tumors induced by human adenovirus type 12. Abstr. (1964). Subject Strain Bibliography 1964. 1087 ...
We use cookies to ensure that we give you the best experience on our website. If you click Continue well assume that you are happy to receive all cookies and you wont see this message again. Click Find out more for information on how to change your cookie settings ...
Berget, S.M., Sharp, P.A. (1977) A spliced sequence at the 5′-terminus of adenovirus late mRNA. Brookhaven Symp Biol, 29:332-44.. Berk, A.J., Sharp, P.A. (1977) Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell 12(3): 721-32.. Chow, L.T., Roberts, J.M., Lewis, J.B., Broker, T.R. (1977) A map of cytoplasmic RNA transcripts from lytic adenovirus type 2, determined by electron microscopy of RNA:DNA hybrids. Cell, 11(4): 819-36.. Gibbs, W.W. (2003) The unseen genome: gems among the junk. Scientific American 289(5):26-33.. Hooks, K. B., Delneri, D. & Grifths-Jones, S. (2014) Intron evolution in Saccharomycetaceae. Genome Biol. Evol. 6, 2543-2556.. Kabat, J.L., Barberan-Soler, S., McKenna, P., Clawson, H., Farrer, T., and Zahler, A.M. (2006) Intronic Alternative Splicing Regulators Identified by Comparative Genomics in Nematodes. PLoS Computational Biology 2(7):734-747.. Kiss, T. and Filipowicz, W. (1995) Genes and Development ...
Adenovirus titration kits accurately measure functional titer of your adenovirus preparation using a hexon antibody, or by SYBR Green-based, real-time qPCR in which the virus samples Ct is compared to a standard curve.
Adenovirus titration kits accurately measure functional titer of your adenovirus preparation using a hexon antibody, or by SYBR Green-based, real-time qPCR in which the virus samples Ct is compared to a standard curve.
Then they inject the adenovirus into a human body. The human immune system learns to recognize the pieces of dangerous viruses ... The adenovirus is the virus that causes the common cold.[1] It does not harm the human body much. Scientists have used the ... They tested APN01 in engineered human tissue. This is human cells put together to act like part of the body, but it is not a ... As of April 2020, it has been tested in mice but not in human beings.[10][11][13][14][15][16][17][18][19] ...
B Human mastadenovirus A Human mastadenovirus B Human mastadenovirus C Human mastadenovirus D Human mastadenovirus E Human ... serotype 14: can cause potentially fatal adenovirus infections. Canine adenovirus 1 (CAdV-1) can lead to death in puppies, or ... Human, mammals, and vertebrates serve as natural hosts. There are currently 25 species in this genus, including the type ... Human, mammals, and vertebrates serve as the natural host. Transmission routes are fecal-oral and respiratory. "Viral Zone". ...
In humans, there are 57 accepted human adenovirus types (HAdV-1 to 57) in seven species (Human adenovirus A to G):[3] ... HumansEdit. Main article: Adenovirus infection. Humans infected with adenoviruses display a wide range of responses, from no ... Genus Mastadenovirus (including all human adenoviruses); type species: Human mastadenovirus C. *Genus Siadenovirus; type ... The two currently established receptors are: CD46 for the group B human adenovirus serotypes and the coxsackievirus adenovirus ...
"Study of Coxsackie B viruses interactions with Coxsackie Adenovirus receptor and Decay-Accelerating Factor using Human CaCo-2 ... Riabi, 2014) When VP1 binds to the Coxsackie-Adenovirus receptor (CAR), which can be found on heart muscle cells as well as ... Dorner, A. A. (2005). "Coxsackievirus-adenovirus receptor (CAR) is essential for early embryonic cardiac development". Journal ... but to date there is no evidence to support this hypothesis in humans. A 2004 systematic review analyzing a possible ...
Tests in mice were followed by tests in humans, beginning in 1989. The first human studies attempted to correct the genetic ... In the same year, Richard Roberts and Phillip Sharp independently showed that the genes of adenovirus contain introns and ... The Medical and Scientific Conceptions of Influenza, Human Virology at Stanford *^ Skloot, Rebecca (2010). The Immortal Life of ... Evans, Alfred (1982). Viral Infections of Humans. New York, NY: Plenum Publishing Corporation. p. xxv-xxxi. ISBN 0306406764.. ...
In general, humans who catch a humanized influenza A virus (a human flu virus of type A) usually have symptoms that include ... "Protection of Mice and Poultry from Lethal H5N1 Avian Influenza Virus through Adenovirus-Based Immunization". J. Virol. 80 (4 ... "While the pandemic human influenza viruses of 1957 (H2N2) and 1968 (H3N2) clearly arose through reassortment between human and ... Influenza A virus subtypes that have been confirmed in humans, in order of the number of known human pandemic deaths that they ...
Adenoviruses can lead to tumors in rodent models but do not cause cancer in humans; however, they have been exploited as ... Human T-lymphotropic virus (HTLV). 0.03. Adult T-cell leukemia[50]. Human papillomaviruses (HPV). 5.2. The types 16 and 18 are ... Human oncoviruses[edit]. *1964: Anthony Epstein, Bert Achong and Yvonne Barr identify the first human oncovirus from Burkitt ... For example, adenoviruses do not cause cancer in humans but are instead responsible for colds, conjunctivitis and other acute ...
The vaccine uses an replication-incompetent human adenovirus.[112]. In July 2020, Johnson & Johnson pledged to deliver up to ... The vaccine candidate entered phase 1 human clinical study in July 2020.[118][119] In September 2020, Johnson & Johnson started ... "XBiotech Announces Agreement to Sell True Human Antibody Bermekimab Targeting IL-1a to Janssen". BioSpace.. ... The mix-up, which federal officials attributed to human error, delayed future shipments of the vaccine.[127] ...
Infection by human adenovirus accounts for 65% to 90% of cases of viral conjunctivitis.[13] ... "Molecular epidemiology of circulating human adenovirus types in acute conjunctivitis cases in Chandigarh, North India". Indian ... An adenovirus was first isolated by Rowe et al. in 1953. Two years later, Jawetz et al. published on epidemic ... Vaccination against adenovirus, Haemophilus influenzae, pneumococcus, and Neisseria meningitidis is also effective.[27] ...
Tsai LH, Harlow E, Meyerson M (septiembre de 1991). «Isolation of the human cdk2 gene that encodes the cyclin A- and adenovirus ... de 1992). «Formation and activation of a cyclin E-cdk2 complex during the G1 phase of the human cell cycle». Science (UNITED ... de 2002). «Human origin recognition complex large subunit is degraded by ubiquitin-mediated proteolysis after initiation of DNA ... de 1997). «Characterization of a second human cyclin A that is highly expressed in testis and in several leukemic cell lines». ...
Liu, H.; Jin, L.; Koh, S. B. S.; Atanasov, I.; Schein, S.; Wu, L.; Zhou, Z. H. (2010). "Atomic Structure of Human Adenovirus by ... Volume of human oocyte - Human Homo sapiens». BioNumbers. دریافت‌شده در ۲۰۱۱-۱۱-۰۱. .. ... Total mass of the human world population[۶۶][۹۴][۹۵] ۵‎×۱۰۱۱ kg Total biomass of کریل جنوبگان, probably the most plentiful ... Surface area and volume of spermatozoa - Human Homo sapiens». BioNumbers. دریافت‌شده در ۲۰۱۱-۱۱-۰۱. .. ...
Yamamoto, Hiroshi; Shimojo, H (August 1971). "Multiplicity reactivation of human adenovirus type 12 and simian virus 40 ... "Simian virus 40 infection in humans and association with human diseases: results and hypotheses". Virology 318 (1): 1-9. PMID ... Simian Virus 40 (SV40:) A Possible Human Polyomavirus Workshop Monday January 27, 1997, Morning Session, transcript of 1997 ... Simian Virus 40 (SV40:) A Possible Human Polyomavirus Workshop Monday January 27, 1997 Afternoon Session, transcript of 1997 ...
"Cloning of the human sodium-iodide symporter promoter and characterization in a differentiated human thyroid cell line, KAT-50 ... using adenoviruses or retroviruses (viral vectors). This genetic technique is called gene targeting. Once NIS is transferred in ... 1991). "Localization of human thyrotropin receptor gene to chromosome region 14q3 by in situ hybridization". Cytogenet. Cell ... 1997). "Increased expression of the Na+/I- symporter in cultured human thyroid cells exposed to thyrotropin and in Graves' ...
Wang C، Chao J، Chao L (Apr 2003). "Adenovirus-mediated human prostasin gene delivery is linked to increased aldosterone ... Yu JX، Chao L، Ward DC، Chao J (Mar 1996). "Structure and chromosomal localization of the human prostasin (PRSS8) gene". ... Yu JX، Chao L، Chao J (Jul 1994). "Prostasin is a novel human serine proteinase from seminal fluid. Purification, tissue ... Yu JX، Chao L، Chao J (Jun 1995). "Molecular cloning, tissue-specific expression, and cellular localization of human prostasin ...
Gene therapy using an adenovirus vector.. Three forms of human enhancement currently exist: reproductive, physical, and mental ... Human enhancement (HE) can be described as the natural, artificial, or technological alteration of the human body in order to ... Human enhancement technologies can impact human identity by affecting one's self-conception.[41] The argument does not ... In some circles the expression "human enhancement" is roughly synonymous with human genetic engineering,[20][21] but most often ...
E19 from some adenoviruses block the movement of MHC I to the proper locations for the endogenous pathway. ... U21 from some human herpesvirus 7 binds and targets certain MHC I molecules for lysosomal degradation. ...
"A novel chimpanzee adenovirus vector with low human seroprevalence: improved systems for vector derivation and comparative ... The adenovirus is called replication-deficient because some of its essential genes were deleted and replaced by a gene coding ... Following vaccination, the adenovirus vector enters the cells and releases its genes, which are transported to the cell nucleus ... The Oxford-AstraZeneca COVID-19 vaccine is a replication-deficient simian adenovirus vector, containing the full‐length codon‐ ...
"Human herpesvirus 7 infection of lymphoid and myeloid cell lines transduced with an adenovirus vector containing the CD4 gene ... Human herpesvirus 7 (HHV-7) is one of nine known members of the Herpesviridae family that infects humans. HHV-7 is a member of ... A 2017 study looking at the human blood virome in 8,240 humans between the ages of 2 months to 102 years found that 20.37% of ... Dewhurst, S (2004). "Human Herpesvirus Type 6 and Human Herpesvirus Type 7 Infections of the Central Nervous System". Herpes: ...
Examples of this are Homo sapiens (human) and Helicobacter pylori. Organisms that show an intermediate level of codon usage ... adenovirus, and parvovirus) are known to encode structural proteins that display heavily skewed codon usage compared to the ...
In humans, currently there are 88 human adenoviruses (HAdVs) in seven species (Human adenovirus A to G): A: 12, 18, 31 B: 3, 7 ... Adenoviruses Stanford University-Adenoviruses Adenoviruses General Concepts General information on Adenovirus DNA virus ... The two currently established receptors are: CD46 for the group B human adenovirus serotypes and the coxsackievirus/adenovirus ... Tupaia adenovirus (TAV) (tree shrew adenovirus 1) has been isolated from tree shrews. Otarine adenovirus 1 has been isolated ...
... from Human Bronchial Epithelial Cells Is Dependent on Nuclear Factor-κB Nuclear Binding and Is Inhibited by Adenovirus-Mediated ... A549 cells are adenocarcinomic human alveolar basal epithelial cells, and constitute a cell line that was first developed in ... "A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate (ab7910)". Retrieved 3 January 2012.. ... "A549 Cell Line: Human alveolar adenocarcinoma cell line -General Information". Retrieved 3 January 2012.. .mw-parser-output ...
"Prevalence of the initiator over the TATA box in human and yeast genes and identification of DNA motifs enriched in human TATA- ... For example, one study used the adenovirus TATA promoter sequence (5'-CGCTATAAAAGGGC-3') as a model binding sequence and found ... One study found less than 30% of 1031 potential promoter regions contain a putative TATA box motif in humans.[9] In Drosophila, ... The DPE has been identified in three Drosophila TATA-less promoters and in the TATA-less human IRF-1 promoter.[10] ...
These tests were used to try to identify potential viruses carried by humans, pigs, or both, including rotoviruses, ... adenoviruses, hepatitis A, and hepatitis E. They also tried to identify bacteria such as salmonella and escherichia coli (e. ... "Ani". mal model mirrors human form of occupational neuropathy in pork plant workers. Neurology Today. Retrieved 19 March 2012. ...
2005). "A human protein-protein interaction network: a resource for annotating the proteome". Cell. 122 (6): 957-68. doi: ... It may play an important role in nucleocytoplasmic RNA transport, and its function is modulated by E1B-55kDa in adenovirus- ... Heterogeneous nuclear ribonucleoprotein U-like protein 1 is a protein that in humans is encoded by the HNRNPUL1 gene. This gene ... 2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci ...
The vaccine uses an replication-incompetent human adenovirus. In July 2020, Johnson & Johnson pledged to deliver up to 300 ... The mix-up, which federal officials attributed to human error, delayed future shipments of the vaccine. In April 2021, federal ... The vaccine candidate entered phase 1 human clinical study in July 2020. In September 2020, Johnson & Johnson started its ... "XBiotech Announces Agreement to Sell True Human Antibody Bermekimab Targeting IL-1a to Janssen". BioSpace. "Janssen to Acquire ...
... and adenovirus 11. AHC can only exist in a human host and is transmitted through human contact with an infected individual or ... Adenoviruses are a medium-sized variant that are nonenveloped, like Enterovirus 70. They have a double-stranded linear DNA ... Its isolation host is human. It is an antigenic variant of the Coxsackievirus A24. It was studied independently for the first ... There are 57 variants of this virus, that each cause 5-10% of upper respiratory infections in humans. These viruses are some of ...
Ras-related GTP binding C, also known as RRAGC, is a protein which in humans is encoded by the RRAGC gene. RRAGC is a monomeric ... CS1 maint: discouraged parameter (link) Horwitz MS (Jan 2001). "Adenovirus immunoregulatory genes and their cellular targets". ... Sekiguchi T, Todaka Y, Wang Y, Hirose E, Nakashima N, Nishimoto T (Feb 2004). "A novel human nucleolar protein, Nop132, binds ... "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine. "Mouse PubMed ...
Kidd, AH; Garwicz D; Oberg M (1995). "Human and simian adenoviruses: phylogenetic inferences from analysis of VA RNA genes". ... These two VA RNA genes are distinct genes in the adenovirus genome. VA RNAI is the major species with VA RNAII expressed at a ... The VA (viral associated) RNA is a type of non-coding RNA found in adenovirus. It plays a role in regulating translation. There ... Ma, Y; Mathews MB (1996). "Secondary and tertiary structure in the central domain of adenovirus type 2 VA RNA I". RNA. 2 (9): ...
"A family of human cdc2-related protein kinases." The EMBO Journal 11.8 (1992): 2909. Whyte, Peter, Nicola M. Williamson, and E ... Whyte, P (1988). "Association between an oncogene and an anti-oncogene: the adenovirus E1A proteins bind to the retinoblastoma ... "The human papilloma virus-16 E7 oncoprotein is able to bind to the retinoblastoma gene product." Science 243.4893 (1989): 934- ... Dyson, N (1989). "The human papilloma virus-16 E7 oncoprotein is able to bind to the retinoblastoma gene product". Science. 243 ...
... adenoviruses]] (5%),,ref name=Medscape /, [[ Virusi sinkytial i frymëmarrjes njerëzore,virusi]] sinkytial i [[ Virusi sinkytial ... Sequencing and Analyses of All Known Human Rhinovirus Genomes Reveals Structure and Evolution,url=,journal=Science,language=en, ...
See Daniel J. Kevles, In the Name of Eugenics: Genetics and the Uses of Human Heredity (Alfred A. Knopf, 1985); Elof A. Carlson ... the adenovirus E1A proteins bind to the retinoblastoma gene product". Nature. 334 (6178): 124-9. Bibcode:1988Natur.334..124W. ... July 2004). "Large-scale copy number polymorphism in the human genome". Science. 305: 525-8. Bibcode:2004Sci...305..525S. doi: ... In 1981 Michael Wigler co-discovers H-RAS, the first human cancer-causing gene, or oncogene; ...
The JC virus or John Cunningham virus is a type of human polyomavirus (formerly known as papovavirus). It was identified by ... Adenovirus Adenovirus infection. RNA virus. Rotavirus. Norovirus. Astrovirus. Coronavirus. Hepatitis. DNA virus. HBV (B). RNA ... Padgett, B.L.; Walker, D.L. (1973). "Prevalence of antibodies in human sera against JC virus, an isolate from a case of ... Pavesi, A. (2005). "Utility of JC polyomavirus in tracing the pattern of human migrations dating to prehistoric times". J. Gen ...
Tetanus toxin is so lethal that humans cannot develop immunity to a natural infection, as the amount of toxin and time required ... Synthetic (recombinant or cell-clone) human immunoglobulins can now be made, and for several reasons (including the risk of ... The earliest recorded artificial induction of immunity in humans was by variolation or inoculation, which is the controlled ... Antiserum from other mammals, notably horses, has been used in humans with generally good and often life-saving results, but ...
Herpesvirus: Cytomegalovirus,[17] Epstein Barr virus,[18] Varicella zoster virus, Human herpesvirus 6, Human herpesvirus 7 ve ... Adenoviridae: Adenovirus. *Arenavirus: Guanarito virus,[2] Junín virus,[2] Lassa fever virus,[2] Lujo virus,[3] Machupo virus[2 ... Arcay L (May 2001). "[Human microsporidiosis]". Invest Clin (Spanish; Castilian). 42 (Suppl 1), s. 3-42. PMID 11416983.. KB1 ... Atías A (June 1995). "[Update on microsporidiosis in humans]". Rev Med Chil (Spanish; Castilian). 123 (6), s. 762-72. PMID ...
It is caused most commonly by Herpes simplex, Herpes zoster and Adenoviruses. Also it can be caused by coronaviruses & many ... Corneal ulcers are a common human eye disease. They are caused by trauma, particularly with vegetable matter, as well as ... It is a common condition in humans particularly in the tropics and the agrarian societies. In developing countries, children ...
... human respiratory syncytial virus, adenoviruses, Enteroviruses, and metapneumovirus.[12] Frequently, more than one virus is ... It binds to human ICAM-1 receptors through unknown methods to trigger the release of inflammatory mediators.[27] These ... Human parainfluenza virus typically results in inflammation of the nose, throat, and airways.[29] In young children, when it ... The common cold is the most common human disease[54] and people are affected all around the world.[16] Adults typically have ...
Cedillo v. Secretary of Health and Human Services. *Alternative vaccination schedule. Related. *Epidemiology ...
Animals are vaccinated to keep them from getting diseases, and to keep them from infecting humans with diseases.[19] Pets as ... adenovirus-2, leptospirosis, bordatella, canine parainfluenza virus, and Lyme disease. ... Olesen OF, Lonnroth A, Mulligan B (2009). "Human vaccine research in the European Union". Vaccine 27 (5): 640-5. doi:10.1016/j. ... Scientists are also working on vaccines against many noninfectious human diseases, such as cancers and autoimmune disorders.[23 ...
B20-B24) Human immunodeficiency virus (HIV) disease[संपादित करें]. *(B20.) Human Immunodeficiency Virus (HIV) disease Resulting ... B97.0) Adenovirus as the cause of diseases classified to other chapters ... B24.) Unspecified Human Immunodeficiency Virus (HIV) Disease. (B25-B34) Other viral diseases[संपादित करें]. *(B25.) ... B21.) Human Immunodeficiency Virus (HIV) disease Resulting in malignant neoplasms *(B21.0) HIV disease resulting in Kaposi's ...
Humans[edit]. Aluminium salts used in many human vaccines are generally regarded as safe.[25] ... Squalene is also produced by the human liver and present in human sebum.[11] MF59 is an oil-in-water emulsion of squalene ... "T cell proliferation by direct cross-talk between OX40 ligand on human mast cells and OX40 on human T cells: comparison of gene ... It is not safe enough for human use. A version without the bacteria, that is only oil in water, is known as Freund's incomplete ...
The vaccine contains a G1P[8] human rotavirus strain.[23] Lanzhou lamb[edit]. Lanzhou lamb rotavirus vaccine was licensed for ... It contain human bovine reassortant strains of rotavirus serotypes G1, G2, G3, G4 and G9. This is world's first thermostable ... It is a live attenuated, monovalent vaccine containing a G9P[11] human strain isolated from an Indian child.[21] It is given by ... O'Ryan M (2007). "Rotarix (RIX4414): an oral human rotavirus vaccine". Expert Rev Vaccines. 6 (1): 11-9. doi:10.1586/14760584.6 ...
Zhou Z, Zwelling LA, Ganapathi R, Kleinerman ES: Enhanced etoposide sensitivity following adenovirus-mediated human ...
Human Papillomavirus නම් වෛරස් එන්නත නිෂ්පාදනය කර ඇත්තේ වෛරසයෙහි කැප්සිඩ ප්‍රෝටීන මගිනි. ...
... is a vaccine that protects in humans against hantavirus infections causing Hantavirus hemorrhagic fever with ... Field trial of an inactivated vaccine against hemorrhagic fever with renal syndrome in humans. Arch Virol. 1990;1(Suppl):35-47. ... Antibody responses in humans to an inactivated hantavirus vaccine (Hantavax). Vaccine. 1999;17:2569-75. ... A phase 2 study on a human HTNV/PUUV DNA hantavirus vaccine is ongoing.[4] ...
Lukes RJ, Collins RD (October 1974). "Immunologic characterization of human malignant lymphomas". Cancer. 34 (4 Suppl): 1488- ... Adenovirus Adenovirus infection. RNA virus. Rotavirus. Norovirus. Astrovirus. Coronavirus. Hepatitis. DNA virus. HBV (B). RNA ...
Ito T، Marintchev A، Wagner G (Sep 2004). "Solution structure of human initiation factor eIF2alpha reveals homology to the ... which can be overcome by helper adenovirus type 5 virus-associated RNA". Journal of Virology. 81 (21): 11908-16. PMC 2168773. ...
... ends of adenovirus 2 messenger RNA". Cell. 12 (1): 1-8. doi:10.1016/0092-8674(77)90180-5. PMID 902310.. ... including human beings.[5][11] ... longer strands of DNA first arose in the study of adenovirus,[ ...
"Epidemic keratoconjunctivitis" is caused by an adenovirus infection.. *"Infectious bovine keratoconjunctivitis" (IBK) is a ...
Cedillo v. Secretary of Health and Human Services. *Alternative vaccination schedule. See also. *Epidemiology ...
Adenovirus Adenovirus infection. RNA virus. Rotavirus. Norovirus. Astrovirus. Coronavirus. Hepatitis. DNA virus. HBV (B). RNA ...
"Human Vaccines. 7. 7: 734-748. doi:10.4161/hv.7.7.15511. PMID 21734466. Nakuha noong 10 May 2012.. Unknown parameter ,month=. ... Gastroenteritis viruses: A = rotavirus, B = adenovirus, C = Norovirus and D = Astrovirus. The virus particles are shown at the ... "Human Vaccines. 6 (7): 532-542. PMID 20622508. Nakuha noong 10 May 2012.. Unknown parameter ,month=. ignored (tulong)CS1 maint ...
"Human Molecular Genetics. 20 (R1): R21-7. doi:10.1093/hmg/ddr137. PMC 3095054. PMID 21459775.. ... adenovirus[150] and measles virus.[151] The other strategy is to block the initial viral entries by targeting the host cell ... 45,000 miRNA target sites within human mRNA 3'UTRs are conserved above background levels, and ,60% of human protein-coding ... It has been estimated from studying the genomes of humans, C. elegans and S. pombe that about 10% of possible siRNAs have ...
Human SRC-1 (steroid receptor coactivator-1) is known to interact with p300/CBP and PCAF, and its HAT domain is located in its ... E1A adenovirus protein, and S-HDAg (hepatitis delta virus small delta antigen).[19] p300/CBP have also been observed to ... Human p300 contains a highly basic loop embedded in the middle of its HAT domain that is hyperacetylated in the active form of ... Human HBO1 (HAT bound to ORC1) was the first HAT shown to associate with components of the origin of replication complex. MORF ...
Aphthous-like ulceration also occurs in conditions involving systemic immuno-dysregulation, e.g. cyclic neutropenia and human ... adenovirus, and cytomegalovirus.[5] Some people with aphthous stomatitis may show herpes virus within the epithelium of the ... and HLA-DR2 are examples of human leukocyte antigen types associated with aphthous stomatitis.[2][5] However, these HLA types ...
Furthermore, depletion of hepatic S6K1 in db/db mice with the use of an adenovirus vector encoding S6K1 shRNA resulted in down- ... 453 in the human promoter. PPARα agonists act in cooperation with LXR or insulin to induce lipogenesis.[9] ... a control mechanism conserved from bacteria to humans". Cell. 100 (4): 391-8. doi:10.1016/S0092-8674(00)80675-3. PMID 10693756. ... RIP was found to be used in almost all organisms from bacteria to human beings and regulates a wide range of processes ranging ...
... as well as viruses such as adenovirus, astrovirus and calicivirus. Other less common pathogens include Bacillus cereus, ... "Human Campylobacteriosis in Developing Countries1". Emerging Infectious Diseases. 8 (3): 237-243. doi:10.3201/eid0803.010233 ...
National Adenovirus Type Reporting System identifies circulation patterns for human adenovirus. ... National Adenovirus Type Reporting System identifies circulation patterns for human adenovirus. ... Distribution of human adenovirus species (HAdVs) and types, by year of specimen collection* - National Adenovirus Type ... The figure above is a bar chart showing the distribution of human adenovirus species and types by the National Adenovirus Type ...
Enhanced expression of p53 in human cells infected with mutant adenoviruses.. Grand RJ1, Grant ML, Gallimore PH. ... The expression of p53 in human cells infected with wild-type (wt) and mutant adenoviruses has been examined. With wt Ad5 and ... the concentration of p53 increased markedly to levels comparable to those seen in adenovirus transformed cells. This increase ...
Researchers from China and the US will sequence 100 human adenoviruses, including ones that cause respiratory, gastrointestinal ... Human adenoviruses were first isolated in 1953 and were initially identified as respiratory pathogens. The DNA viruses are now ... BGI Partners with US Researchers to Sequence 100 Human Adenoviruses May 02, 2012 , staff reporter ... NEW YORK (GenomeWeb News) - Researchers from China and the US will sequence 100 human adenoviruses, including ones that cause ...
This review attempts to address the importance of developing pharmaceuticals for adenovirus and also review recent development ... in drug discovery for adenovirus, including newer strategies such as microRNA approaches. Different drug screening strategies ... many stages in the development of a new drug for viral infection and such processes are even further complicated for adenovirus ... Adenovirus Biology and Structure. An adenovirus is a non-enveloped, dsDNA virus. There are at least 51 human Adenovirus ...
DNA from Human adenovirus 4 strain RI-67 [ATCC ® VR-1572™] Application: It is suitable for use in polymerase chain reaction ( ... DNA from Human adenovirus 4 strain RI-67 [ATCC® VR-1572™] (ATCC® VR-1572D™) Organism: Human adenovirus 4 / Focus: Infectious ... DNA from Human adenovirus 4 strain RI-67 [ATCC® VR-1572™] ATCC® VR-1572D™ frozen 100 µL per vial ... infected with Human adenovirus 4 (HAdV-4) strain RI-67, (ATCC® VR-1572™). It is supplied at 100 µL per vial, shipped frozen. It ...
DNA from Human adenovirus 12 strain Huie [ATCC ® VR-863™] Application: It is suitable for use in polymerase chain reaction (PCR ... DNA from Human adenovirus 12 strain Huie [ATCC® VR-863™] (ATCC® VR-863D™) Organism: Human adenovirus 12 / Focus: Infectious ... infected with Human adenovirus 12 (HAdV-12) strain Huie (ATCC® VR-863™). It is supplied at 100 µL per vial, shipped frozen. It ...
... Scientific Reports 3:1812. Abstract. The recent emergence of highly virulent human ... suggesting foci of DNA instability lead to formulaic patterns of homologous recombination and confer agility to adenovirus ... adenoviruses (HAdVs) with new tissue tropisms underscores the need to determine their ontogeny. Here we report complete high ...
Computational Analysis Identifies Human Adenovirus Type 55 as a Re-Emergent Acute Respiratory Disease Pathogen Michael P. Walsh ... Quantitative Detection and Rapid Identification of Human Adenoviruses Rika Miura-Ochiai, Yasushi Shimada, Tsunetada Konno, ... Pseudo-Outbreak of Adenovirus Infection in a Neonatal Intensive Care Unit Due to a False-Positive Antigen Detection Test Howard ... Genomic Analyses of Recombinant Adenovirus Type 11a in China Zhaohui Yang, Zhen Zhu, Liuying Tang, Li Wang, Xiaojuan Tan, ...
Rapid and quantitative detection of human adenovirus DNA by real-time PCR.. Heim A1, Ebnet C, Harste G, Pring-Akerblom P. ... Rapid diagnosis of human adenovirus (HAdV) infections was achieved by PCR in the recent years. However, conventional PCR has ... Adenovirus viremia was detected by TaqMan PCR in 4 of 27 (14.8%) paediatric and 8 of 93 (8.6%) adult stem cell transplant ... In conclusion, real-time PCR is a sensitive and quantitative procedure for the detection of adenovirus infections. ...
Drug: Recombinant Human Adenovirus Type 5 Injection After identifying the target artery of HCC, Recombinant Human Adenovirus ... Recombinant Human Adenovirus Type 5, an E1B gene deleted adenovirus, is known to have a significant antitumor activity. In ... Experimental: TACE Plus Adenovirus After identifying the target artery of HCC, Recombinant Human Adenovirus Type 5 Injection( ... TACE Plus Recombinant Human Adenovirus for Hepatocellular Carcinoma. Official Title ICMJE Phase Ⅲ Trial of Transcatheter ...
Human adenovirus 12. Other names i. ›Adenovirus Ad12. ›Adenovirus type 12. ›Human adenovirus type 12. ›Mastadenovirus 12. › ... Taxonomy - Human adenovirus A serotype 12 (HAdV-12) (Human adenovirus 12) Basket 0 ...
Human adenovirus A serotype 12 (HAdV-12) (Human adenovirus 12). ,p>This subsection of the ,a href="http://www.uniprot.org/help/ ... sp,P03259,E1A_ADE12 Early E1A protein OS=Human adenovirus A serotype 12 OX=28282 PE=1 SV=1 ... "mUBC9, a novel adenovirus E1A-interacting protein that complements a yeast cell cycle defect.". Hateboer G., Hijmans E.M., ... "mUBC9, a novel adenovirus E1A-interacting protein that complements a yeast cell cycle defect.". Hateboer G., Hijmans E.M., ...
There are no specific protocols for Recombinant Human Coxsackie Adenovirus Receptor protein (ab114429). Please download our ...
adenovirus;. CVB,. group B coxsackieviruses;. CMV,. cytomegalovirus;. HCAR,. human CVB and Ad2 and 5 receptor;. MCAR,. mouse ... However, adenovirus (Ad) serotypes 2 (Ad2) and 5 (Ad5) and the parental group B coxsackieviruses (CVB) are human pathogens that ... HCAR and MCAR: The human and mouse cellular receptors for subgroup C adenoviruses and group B coxsackieviruses. Richard P. ... HCAR and MCAR: The human and mouse cellular receptors for subgroup C adenoviruses and group B coxsackieviruses ...
Drug: Recombinant Human Adenovirus Type 5 Injection After identifying the target artery of HCC, Recombinant Human Adenovirus ... Recombinant Human Adenovirus Type 5, an E1B gene deleted adenovirus, is known to have a significant antitumor activity. In ... Experimental: TACE Plus Adenovirus After identifying the target artery of HCC, Recombinant Human Adenovirus Type 5 Injection( ... TACE Plus Recombinant Human Adenovirus for Hepatocellular Carcinoma. The safety and scientific validity of this study is the ...
Upon transfection of salivary glands, adenovirus encoding human aquaporin-1 (AdhAQP1) directs human aquaporin-1 (hAQP1) ... recombinant adenovirus encoding human aquaporin-1 with potential membrane water channel activity. ... adenovirus encoding human aquaporin-1 A replication-deficient, recombinant adenovirus encoding human aquaporin-1 with potential ... Upon transfection of salivary glands, adenovirus encoding human aquaporin-1 (AdhAQP1) directs human aquaporin-1 (hAQP1) ...
... R. Moreno,1 L. A. ... "Human mesenchymal stem cells lack tumor tropism but enhance the antitumor activity of oncolytic adenoviruses in orthotopic lung ... A. Rosewell Shaw and M. Suzuki, "Recent advances in oncolytic adenovirus therapies for cancer," Current Opinion in Virology, ... R. W. Chan, K. E. Schwab, and C. E. Gargett, "Clonogenicity of human endometrial epithelial and stromal cells," Biology of ...
Transcriptional Targeting of Recombinant Adenoviruses to Human and Murine Melanoma Cells. William M. Siders, Patrick J. ... Transcriptional Targeting of Recombinant Adenoviruses to Human and Murine Melanoma Cells. William M. Siders, Patrick J. ... Transcriptional Targeting of Recombinant Adenoviruses to Human and Murine Melanoma Cells. William M. Siders, Patrick J. ... Transcriptional Targeting of Recombinant Adenoviruses to Human and Murine Melanoma Cells Message Subject (Your Name) has ...
... a more virulent strain of human adenovirus arose from recombination with other distinct strains of milder human adenoviruses. ... Adenoviruses commonly infect humans, causing colds, flu-like symptoms and sometimes even death, but now UC San Francisco ... "Adenoviruses to date have not generally been linked to cross-species infections between monkeys and humans," Chiu said. ... Last year, Chiu and colleagues also identified another new adenovirus, named simian adenovirus C, which sickened four of nine ...
Presented are ways to address the problem of replication competent adenovirus in adenoviral production for use with, for ... 0030] These cells cannot be used directly for the production of recombinant human adenovirus, as human adenovirus cannot ... The newly generated human adenovirus packaging cell lines or cell lines derived from species permissive for human adenovirus ( ... Source of adenovirus sequences. [0117] Adenovirus sequences are derived either from pAd5.SalB containing nt. 80-9460 of human ...
Immunological Relationships Between Hexons of Certain Human Adenoviruses Message Subject (Your Name) has forwarded a page to ... Immunological Relationships Between Hexons of Certain Human Adenoviruses. Erling Norrby, Göran Wadell ... were absorbed with purified hexons of various serotypes representing the different subgroups of human adenoviruses. Group, ... This question is for testing whether or not you are a human visitor and to prevent automated spam submissions. ...
New human adenovirus isolated from a renal transplant recipient: description and characterization of candiate adenovirus type ... Latent Species C Adenoviruses in Human Tonsil Tissues. C. T. Garnett, G. Talekar, J. A. Mahr, W. Huang, Y. Zhang, D. A. ... Latent Species C Adenoviruses in Human Tonsil Tissues. C. T. Garnett, G. Talekar, J. A. Mahr, W. Huang, Y. Zhang, D. A. ... Latent Species C Adenoviruses in Human Tonsil Tissues. C. T. Garnett, G. Talekar, J. A. Mahr, W. Huang, Y. Zhang, D. A. ...
... transformed with adenovirus 5 DNA ) from GeneTex,Human 293 cells were cultured in Minimum Essential Medium (Eagle) with 2 mM L- ... A - 549 cell line slides ( human: lung; carcinoma ) from GeneTex. 11. Raji cell line slides ( human: B lymphocyte; Burkitts ... 293 cell line slides ( human: kidney; transformed with adenovirus 5 DNA ) from GeneTex. ... Human 293 cells were cultured in Minimum Essential Medium (Eagle) with 2 mM L-glutamine and harvested at the log phase of ...
2. Human adenovirus infection counteracts the anti-viral activity of the cellular MKRN1 E3 ubiquitin ligase. Open this ... Human adenovirus (HAdV) is a common pathogen causing a broad spectrum of diseases. HAdV encodes the pVII protein, which is ... 4. RNA triplex formation in human adenovirus type 4 VA RNAI and its implication on virus growth. Open this publication in new ... Open this publication in new window or tab ,,Complementation of the human adenovirus type 5 VA RNAI defect by the Vaccinia ...
These diseases associated with adenovirus infection affect adults and are usually more severe in infant ... Adenoviruses are known to cause several human diseases including acute febrile respiratory syndromes, epidemic conjunctivitis ... Forty-seven human adenoviruses serotypes have so far been identified adenovirus. The diversity of these viruses has delayed ... kit designed for diagnosis of human enteric adenoviruses in stool samples.. RESULTS: The highest prevalence of adenoviruses, ...
... but now UC San Francisco researchers have discovered that a new species of adenovirus can spread from primate to primate, and ... Adenoviruses commonly infect humans, causing colds, flu-like symptoms and sometimes even death, ... Adenoviruses may pose risk for monkey-to-human leap. July 25, 2013, University of California, San Francisco Adenoviruses ... While human adenoviruses typically cause mild infections, recent reports have described newly characterized adenoviruses that ...
Magwalivha M, Wolfaardt M, Kiulia NM, van Zyl WB, Mwenda JM, Taylor MB, High prevalence of species D human adenoviruses in ... Liu EB, Ferreyra L, Fischer SL, Pavan JV, Nates SV, Hudson NR, Genetic analysis of a novel human adenovirus with a ... Genomic characterization of a novel human adenovirus type 31 recombinant in the hexon gene. J Gen Virol. 2011;92:2770-5. DOI ... Molecular evolution of human species D adenoviruses. Infect Genet Evol. 2011;11:1208-17. DOIPubMed ...
... and a replication-deficient adenovirus containing the genomic sequence of human CYP21 (hAdCYP21). Intra-adrenal injection of ... Adenovirus mediated expression of therapeutic plasma levels of human factor IX in mice Nat Genet 1993 5: 397-402 ... Tajima, T., Okada, T., Ma, X. et al. Restoration of adrenal steroidogenesis by adenovirus-mediated transfer of human ... Restoration of adrenal steroidogenesis by adenovirus-mediated transfer of human cytochromeP450 21-hydroxylase into the adrenal ...
EKC is a severe form of acute conjunctivitis caused by human adenoviruses (HAdVs). Clinical illness typically lasts 1 to 3 ... Notes from the Field: Epidemic Keratoconjunctivitis Outbreak Associated with Human Adenovirus Type 8 - U.S. Virgin Islands, ... Notes from the Field: Epidemic Keratoconjunctivitis Outbreak Associated with Human Adenovirus Type 8 - U.S. Virgin Islands, ... or laboratory confirmation of human adenovirus type 8 from a specimen collected by conjunctival swab during June 1-November 29 ...
  • There are many stages in the development of a new drug for viral infection and such processes are even further complicated for adenovirus by the fact that there are at least 51 serotypes, forming six distinct groups (A-F), with different degree of infectivity. (mdpi.com)
  • There are at least 51 human Adenovirus serotypes which are classified into six groups (A to F) based on their biochemical, immunological and morphological criteria. (mdpi.com)
  • However, adenovirus (Ad) serotypes 2 (Ad2) and 5 (Ad5) and the parental group B coxsackieviruses (CVB) are human pathogens that obviously share a common receptor although they belong to divergent virus families ( 1 ). (pnas.org)
  • Antisera against hexons of serotypes 2, 4, 5, and 6 (subgroup III), and 15 (subgroup II) were absorbed with purified hexons of various serotypes representing the different subgroups of human adenoviruses. (asm.org)
  • All species C serotypes were represented in these tissues, although adenovirus type 6 was notably rare. (asm.org)
  • The four species C serotypes (adenovirus type 1 [Ad1], Ad2, Ad5, and Ad6) are the most commonly encountered of the 51 known human adenoviruses. (asm.org)
  • In a 6-year prospective study, adenoviruses were found in 8% of nasal washings from children with febrile respiratory illness, 81% of which were species C serotypes 1 or 2 ( 11 ). (asm.org)
  • Forty-seven human adenoviruses serotypes have so far been identified adenovirus. (biomedsearch.com)
  • To develop real-time PCR for adenoviruses, degenerate primers and a Taqman probe targeting a 163-bp region of the adenovirus hexon gene were designed to specifically amplify 14 different serotypes of human adenoviruses, including enteric adenovirus serotype 40 and 41. (asm.org)
  • Epidemic keratoconjunctivitis is a hyperacute and highly contagious infection of the eye caused by human adenovirus types within species D, in particular, serotypes D8, D19, and D37. (arvojournals.org)
  • PCR analysis of egyptian respiratory adenovirus isolates, including identification of species, serotypes, and coinfections. (semanticscholar.org)
  • Therefore, alternative adenovirus serotypes are being explored. (surrey.ac.uk)
  • Comparative sequence analysis of the hexon gene in the entire spectrum of human adenovirus serotypes: phylogenetic, taxonomic, and clinical implications. (microbiologyresearch.org)
  • There are at least 52 immunologically distinct serotypes of adenoviruses that can cause human infections. (health.mil)
  • In the United States, adenovirus serotypes 4 and 7 most commonly cause respiratory illness. (health.mil)
  • Some adenovirus serotypes can establish persistent asymptomatic infections in tonsils, adenoids, and intestines of infected people who can shed these viruses for months or years. (health.mil)
  • Based on data from the National Adenovirus Type Reporting System, the most commonly reported types of HAdVs during 2003-2016 in the United States were HAdV types 1, 2, 3, 4, 7, and 14, which accounted for 85.5% (n = 1,283) of all types reported. (cdc.gov)
  • CDC summarized HAdV detections voluntarily reported through the National Adenovirus Type Reporting System (NATRS) after initiation of surveillance in 2014 to describe trends in reported HAdVs circulating in the United States. (cdc.gov)
  • Patterns of alternating GC and AT rich motifs correlated well with hypervariable region recombination sites across the HAdV-D genomes, suggesting foci of DNA instability lead to formulaic patterns of homologous recombination and confer agility to adenovirus evolution. (harvard.edu)
  • Rapid diagnosis of human adenovirus (HAdV) infections was achieved by PCR in the recent years. (nih.gov)
  • Transverse chest CT images demonstrated in- We identified a case of fatal acute respiratory disease creased areas of patchy shadows and consolidation in both from household transmission of human adenovirus type lungs compared to CT images from April 22, indicative of 55 (HAdV-55) in Anhui Province, China. (cdc.gov)
  • Human adenovirus (HAdV) is a common pathogen causing a broad spectrum of diseases. (diva-portal.org)
  • We report a novel human adenovirus D (HAdV-65) isolated from feces of 4 children in Bangladesh who had acute gastroenteritis. (cdc.gov)
  • HAdV infection is also associated with a serious adenovirus syndrome in immunocompromised patients after stem cell transplantation ( 2 ). (cdc.gov)
  • Phylogenetic trees of a novel human adenovirus (HAdV-65), 3 strains (DC 11, DC 253, and DC 303), and other HAdV-D reference strains. (cdc.gov)
  • Human Adenovirus (HAdV) are responsible for severe infections in hematopoietic stem cells transplant (HSCT) recipient, species C viruses being the most commonly observed in this population. (ovid.com)
  • Human adenovirus (HAdV) has been recognized as a significant viral pathogen implicated in neurological diseases, particularly in immunocompromised patients. (springer.com)
  • Using a library of hamster cells expressing specific human integrins, along with recombinant soluble penton base from HAdV type 41 and commercially available soluble laminins, we identified laminin-binding integrins as co-receptors for entry and infection of human intestinal HT-29 cells by the enteric HAdVs. (diva-portal.org)
  • Human adenoviruses (HAdV) and hepatitis A virus (HAV) are shed in the faeces and consequently may be present in environmental waters, resulting in an increase in pathogen concentration that can affect water quality and human health. (scielo.br)
  • Human adenovirus (HAdV) is one of the most common pathogens associated with viral ARI, and thus calls for specific diagnosis and better understanding of the epidemiology and clinical characteristics. (plos.org)
  • Background: Human adenovirus (HAdV) causes acute diarrhoea sporadically, as well as in outbreaks. (uib.no)
  • This first report on molecular epidemiology of human adenovirus in Tanzania observed diversity of HAdV types that circulate the study setting. (uib.no)
  • Based on the analysis of its complete genome sequence, the novel complex recombinant Human mastadenovirus D (HAdV-D) strain was classified into a new HAdV type: HAdV-84, and it was designated Adenovirus D human/PAN/P309886/2011/84[P43H17F84]. (diva-portal.org)
  • In our study, we used physical, pharmacological, biochemical, fluorescence and electron microscopy, molecular and cell biology approaches to dissect the impact of immune-complexed HAdV (IC-HAdV) on human monocyte-derived dendritic cells (MoDCs). (cnrs.fr)
  • Our study provides mechanistic insight into how humoral immunity initiates an innate immune response to HAdV-C5 in human professional APCs. (cnrs.fr)
  • Human adenoviruses of species D (HAdV-D) can be associated with acute respiratory illness, epidemic keratoconjunctivitis, and gastroenteritis, but subclinical HAdV-D infections with prolonged shedding have also been observed, particularly in immunocompromised hosts. (altmetric.com)
  • To expand knowledge on HAdV-D in Sub-Saharan Africa, we investigated the prevalence, epidemiology and pathogenic potential of HAdV-D in humans from rural areas of 4 Sub-Saharan countries, Côte d'Ivoire (CI), Democratic Republic of the Congo (DRC), Central African Republic (CAR) and Uganda (UG). (altmetric.com)
  • Human adenovirus type 34 (HAdV-34) infection is a recognized cause of transplant-associated hemorrhagic cystitis and, in rare cases, tubulointerstitial nephritis. (jefferson.edu)
  • Although a few immunocompetent animal models to study the immune response against human adenoviruses (HAdV) are available, such as Syrian hamsters and cotton rats, HAdV replication is several logs lower compared to human control cells. (diva-portal.org)
  • We have identified a non-transformed mouse epithelial cell line (NMuMG) where HAdV-2 gene expression and progeny formation was as efficient as in the highly permissive human A549 cells. (diva-portal.org)
  • A novel human recombinant adenovirus of species A (HAdV-A31 MZ) was isolated from a patient with acute gastroenteritis in Japan. (microbiologyresearch.org)
  • In order to understand the evolution of adenoviruses correctly and to avoid misidentification of HAdV types, we recommend characterizing not only the hexon gene, but also the penton base and fiber genes. (microbiologyresearch.org)
  • 60 genotypes are known to cause human infection ( 1 ). (cdc.gov)
  • For example, a recent outbreak of respiratory infections in China was initially believed to be SARS-related, but genomic analysis pinned the outbreak as a respiratory tract infection cause by adenovirus type 55, the collaborators said. (genomeweb.com)
  • Infection by adenovirus starts with the binding of fibers to a specific receptor on the cell surface. (mdpi.com)
  • daughter of AQ-1, had a febrile respiratory infection with Human Adenovirus Type 55 cough. (cdc.gov)
  • In contrast, viruses that use DNA as their genetic material, such as adenoviruses, are thought to have less chance of spreading between species because they replicate with fewer mutations that could serve as the basis for infection-enhancing changes. (healthcanal.com)
  • In this study, human tonsil and adenoid tissues were analyzed to determine the dynamics of infection, the rate of clearance of viral DNA, and the possibility of reactivation of virus from these tissues. (asm.org)
  • Recently we reported a surprising association between prenatal species C adenovirus infection and the development of childhood acute leukemia ( 23 ). (asm.org)
  • These diseases associated with adenovirus infection affect adults and are usually more severe in infants and children. (biomedsearch.com)
  • To date, limited studies have been done to determine the prevalence of adenovirus infection in non-human primates with the goal of developing a non-human primate model that can be used to study the mechanisms of infection. (biomedsearch.com)
  • OBJECTIVE: To determine the prevalence of enteric adenovirus infection in Kenyan non-human primates. (biomedsearch.com)
  • DESIGN: A prospective study to investigate the prevalence of enteric andenovirus infection in captive non-human primates maintained in a colony. (biomedsearch.com)
  • 0.05) in adenovirus infection signifying equal susceptibility. (biomedsearch.com)
  • CONCLUSION: The results of this study indicate that adenovirus infection is prevalent among non-human primates in Kenya. (biomedsearch.com)
  • Some epidemiological studies have suggested a possible link between human cytomegalovirus (HCMV) infection and various malignancies, and HCMV has been shown to transform cultured cells. (pnas.org)
  • However, if HCMV infection does contribute to tumor induction in humans, the mechanism underlying HCMV-induced oncogenesis is very likely different from that of DNA viruses that are known to play a role in human malignancies. (pnas.org)
  • In this study, we evaluated valganciclovir against disseminated adenovirus type 5 (Ad5) infection in our permissive immunosuppressed Syrian hamster model. (mdpi.com)
  • are members of the normal floras of the gastrointestinal tract in human and animals and have emerged as a leading cause of nosocomial infection ( 12 , 29 ). (asm.org)
  • We report here the novel finding that human erythrocytes present Coxsackie virus-adenovirus receptor (CAR) providing an Ad5 sequestration mechanism that protects against systemic infection. (bloodjournal.org)
  • Erythrocytes may therefore restrict Ad5 infection (natural and therapeutic) in humans, independent of antibody status, presenting a formidable challenge to Ad5 therapeutics. (bloodjournal.org)
  • In a mouse model of GSD-II, we demonstrate that infection of the murine liver with a modified adenovirus (Ad) vector encoding human GAA (hGAA) resulted in long-term persistence of the vector in liver tissues for at least 6 months. (unboundmedicine.com)
  • There was no evidence for an increased prevalence of infection with a closely related adenovirus, adenovirus 18, or another enteric virus, Echovirus 11, among coeliac disease subjects. (bmj.com)
  • In addition, epidemiological features and co-infection with other human respiratory pathogens were investigated and analyzed. (plos.org)
  • Conclusion: The present study provides further evidence of the contribution of adenovirus in causing gastroenteritis in young children, with symptomatic infection being significantly more prevalent in children below one year. (uib.no)
  • Long Term Changes in Adiposity and Glycemic Control Are Associated With Past Adenovirus Infection. (medigraphic.com)
  • Antibodies to adenoviruses are common in the human population due to natural infection. (aacrjournals.org)
  • Epidemiological analysis and follow-up of human rhinovirus infection in children with asthma exacerbation. (semanticscholar.org)
  • The infection of epithelia] swine testicle and intestinal porcine epithelial (IPEC-1) cell lines by adenovirus type 5 (Ad5) has been studied in vitro by using an Ad5-luciferase recombinant containing the firefly luciferase gene as a reporter. (asm.org)
  • These results taken together indicate that swine may be a good animal model for human Ad5 lung infection to aid in the evaluation of candidate adenovirus vaccines and that Ad5 may be suitable as a recombinant viral vaccine or for other applications in swine. (asm.org)
  • Immunization with human papillomavirus (HPV) L1 virus-like particles (VLPs) prevents infection with HPV. (asm.org)
  • Since enteroviral infection of beta cells has been implicated as a factor that could contribute to the development of type 1 diabetes, it is often assumed that CAR is displayed on the surface of human beta cells. (jyu.fi)
  • Probable Donor-Derived Human Adenovirus Type 34 Infection in 2 Kidney " by Matthew A. Pettengill, Tara M. Babu et al. (jefferson.edu)
  • Probable Donor-Derived Human Adenovirus Type 34 Infection in 2 Kidney Transplant Recipients From the Same Donor. (jefferson.edu)
  • End-modified poly(β-amino ester)s or easy-to-synthesize degradable polymers, that are able to deliver DNA to primary human umbilical vein endothelial cells (HUVEC) at levels comparable to adenovirus at a Multiplicity of Infection (MOI) between 100 and 500, are developed. (elsevier.com)
  • Respiratory illness symptoms caused by adenovirus infection range from common cold symptoms, pharyngitis, and rhinitis, to bronchitis or pneumonia. (health.mil)
  • Young infants and patients with compromised immune systems are more susceptible to severe complications from adenovirus infection. (health.mil)
  • What are the symptoms of adenovirus infection? (health.mil)
  • Strict attention to good infection-control practices, including contact and droplet precautions is effective for stopping nosocomial outbreaks of adenovirus associated disease. (health.mil)
  • Human adenoviruses (HAdVs) can cause a wide spectrum of clinical illness, ranging from asymptomatic infections to severe illnesses and death. (cdc.gov)
  • The recent emergence of highly virulent human adenoviruses (HAdVs) with new tissue tropisms underscores the need to determine their ontogeny. (harvard.edu)
  • Human adenoviruses (HAdVs) are common pathogens that cause several diseases, such as pneumonia, acute gastroenteritis, and epidemic keratoconjunctivitis ( 1 ). (cdc.gov)
  • EKC is a severe form of acute conjunctivitis caused by human adenoviruses (HAdVs). (cdc.gov)
  • Human adenoviruses (HAdVs) cause respiratory illnesses, epidemic conjunctivitis and infantile gastroenteritis. (diva-portal.org)
  • Human adenoviruses (HAdVs) are nonenveloped proteinaceous particles containing a linear double-stranded DNA genome. (cnrs.fr)
  • Background Human Adenoviruses (HAdVs) cause a wide array of illnesses in all age groups. (researchsquare.com)
  • Human adenoviruses have been implicated as infectious agents which are responsible for numerous diseases, including respiratory tract infections, ocular and gastrointestinal tract disorders [ 1 ]. (mdpi.com)
  • In conclusion, real-time PCR is a sensitive and quantitative procedure for the detection of adenovirus infections. (nih.gov)
  • In addition, HCAR and MCAR mRNAs could be detected on Northern blots of oligo(dT)-selected RNA from receptor-positive HeLa cells and TCMK-1 as well as several tissues of human and mouse origin that are known to be targets for Ad and CVB infections. (pnas.org)
  • Adenoviruses to date have not generally been linked to cross-species infections between monkeys and humans," Chiu said. (healthcanal.com)
  • Although species C human adenoviruses establish persistent infections, the molecular details of this lifestyle remain poorly understood. (asm.org)
  • These data suggest that species C adenoviruses can establish latent infections in mucosal lymphocytes and that stimulation of these cells can cause viral reactivation resulting in RNA transcription, DNA replication, and infectious virus production. (asm.org)
  • Species C adenoviruses are normally weakly pathogenic, with a frequency of asymptomatic primary infections of 50 to 90% ( 3 , 11 , 18 ). (asm.org)
  • Adenovirus infections of very young children can lead to fatal pneumonia ( 8 , 33 , 50 ). (asm.org)
  • Species C adenoviruses also establish persistent infections characterized by intermittent excretion ( 19 ). (asm.org)
  • Adenovirus infections of immunocompromised pediatric hematopoietic stem cell transplant patients can develop into serious and often deadly multi-organ disease. (mdpi.com)
  • There are no drugs approved for adenovirus infections. (mdpi.com)
  • Our results support the use of valganciclovir to treat disseminated adenovirus infections in immunosuppressed patients. (mdpi.com)
  • Avellón A, Pérez P, Aguilar JC, Lejarazu R, Echevarrı́a JE (2001) Rapid and sensitive diagnosis of human adenovirus infections by a generic polymerase chain reaction. (springer.com)
  • There are two major pathogenic species in human, Enterococcus faecalis ( E. faecalis ) and E. faecium , with occasional infections being caused by E. durans , E. gallinarum , E. casseliflavus , E. avium , E. hirae , E. mundtii , and E. raffinosus ( 11 , 32 ). (asm.org)
  • Adenovirus infections occur year-round, and there is little or no seasonal variation in shedding ( 1 ). (asm.org)
  • Persistent enteral infections with adenovirus types 1 and 2 in infants: no evidence of reinfection. (microbiologyresearch.org)
  • Conclusion All ages suffer adenovirus infections, but young children are at greatest risk. (researchsquare.com)
  • The subgroup C of the adenoviruses (Ad) and the group B coxsackieviruses (CVB) are structurally unrelated viruses that are known to compete for an unidentified cell surface receptor. (pnas.org)
  • We now describe the isolation of cDNAs from human and mouse that encode the human CVB and Ad2 and 5 receptor (HCAR) and the mouse CVB Ad2 and 5 receptor (MCAR). (pnas.org)
  • Coxsackievirus and adenovirus receptor, CD46, sialic acid, coagulation factors IX and X, lactoferrin and heparan sulfate are some receptors and molecules which the hexon and fiber proteins (components of the capsid) bind for direct or indirect cellular attachment. (diva-portal.org)
  • The crystal structure of the adenovirus fiber knob domain in complex with domain I of its human cellular receptor, coxsackie and adenovirus receptor (CAR), is presented here. (sciencemag.org)
  • Human, but not murine, erythrocytes also present complement receptor (CR1), which binds Ad5 in the presence of antibodies and complement. (bloodjournal.org)
  • Human adenovirus type 26 uses sialic acid as a primary cellular receptor-structural insights for this phase 3 vaccine vector. (sciencemag.org)
  • 0.05) in transgenic mice overexpressing human B 2 receptor compared with wild-type control mice (c57/Bl6 mice). (ahajournals.org)
  • Aims/hypothesis The Coxsackie and adenovirus receptor (CAR) is a transmembrane cell-adhesion protein that serves as an entry receptor for enteroviruses and may be essential for their ability to infect cells. (jyu.fi)
  • In addition, several target cells, such as endothelial and smooth muscle cells, are difficult to transduce by Ad5 due to the low expression of the primary coxsackie-adenovirus receptor (CAR). (surrey.ac.uk)
  • We have also shown that adenovirus interferes with adhesion of platelets to a fibronectin-coated surface and flow cytometry revealed the presence of the Coxsackie adenovirus receptor on the platelet surface. (bloodjournal.org)
  • Coxsackievirus and adenovirus receptor (CAR) is a protein that in humans is encoded by the CXADR gene. (wikipedia.org)
  • The protein encoded by this gene is a type I membrane receptor for group B coxsackie viruses and subgroup C adenoviruses. (wikipedia.org)
  • These data provide evidence that it is possible to target human melanoma cells with great efficiency and specificity using high-titer recombinant adenovirus vectors. (aacrjournals.org)
  • Adenoviral vectors have been used to transfer genes to cells in order to correct for genetic deficiencies in animal models and humans. (nature.com)
  • 12, 13 Since steroids are anti-inflammatory, 14 we sought the possibility that the high content of corticosteroids in the adrenal gland may prevent the immune reaction to adenovirus injection and make it possible to use these vectors for gene therapy in the adrenal. (nature.com)
  • Both glioma cells and endothelium in the tumor blood vessels were transduced with retro- and adenovirus vectors. (nih.gov)
  • In conclusion, the safety and feasibility of in vivo gene transfer to human malignant glioma was established with retro- and adenovirus vectors. (nih.gov)
  • To express the gene pol of human adenovirus D36 ( pol-D36 ) in the model cell lines a set of recombinant lentiviral vectors was constructed, containing pol-D36 and tdTomato (tandem tomato fluorescent protein) cDNAs separated by an IRES sequence. (arvojournals.org)
  • To further characterize the potential of this vector as a vaccine we designed replication deficient hAd19a, hAd5 and MVA vectors expressing a papillomavirus (PV) antigen fused to the human MHC class II associated invariant chain T cell adjuvant (hIi) and investigated their immunogenicity in vivo in mice and cynomolgus macaques. (uni-muenchen.de)
  • The structure identifies key determinants of binding specificity, which may suggest ways to modify the tropism of adenovirus-based gene therapy vectors. (sciencemag.org)
  • To define the bottlenecks that restrict antigen expression after oral administration of viral-vectored vaccines, we tracked vectors derived from the human adenovirus type 5 at whole body, tissue, and cellular scales throughout the digestive tract in a murine model of oral delivery. (frontiersin.org)
  • Tropism of human adenovirus type 5-based vectors in swine and their ability to protect against transmissible gastroenteritis coronavirus. (asm.org)
  • The endostatin expression in a cultured supernatant from tumor cells was significantly higher than that obtained from non-duplicative adenovirus vectors carrying that gene. (lu.se)
  • BACKGROUND: For adenovirus vectors derived from human serotype 5 (Ad5), the efficiency and safety after intravascular delivery is hindered by their sequestration in nontarget tissues, predominantly the liver. (surrey.ac.uk)
  • These polymer modifications dramatically enhance DNA delivery to ovarian tumors, and the development of polymeric vectors with gene delivery efficacy comparable to adenovirus could set a new benchmark in non- viral transfection capability. (elsevier.com)
  • Following systemic injection into mice bearing subcutaneous or orthotopic prostate tumors, cotransduced macrophages migrated into hypoxic tumor areas, upregulated E1A protein, and released multiple copies of adenovirus. (aacrjournals.org)
  • The fact that the IE1 and IE2 gene products are able to inhibit apoptosis raised the interesting possibility that they might cooperate with the adenovirus E1A oncoproteins to transform primary rodent cells, as do the adenovirus E1B 19-kDa (E1B) protein and the cellular Bcl-2 protein, each of which can block apoptosis ( 15 , 16 ). (pnas.org)
  • Association of human class I MHC alleles with the adenovirus E3/19K protein. (jimmunol.org)
  • A panel of HLA-A and -B locus products was analyzed for their ability to associate with the adenovirus E3/19K (E19) protein in a co-immunoprecipitation assay. (jimmunol.org)
  • CAR is an integral membrane protein of unknown cellular function that is expressed in a wide range of human and murine cell types. (sciencemag.org)
  • The knob domain from Ad serotype 12 (Ad12) and the NH 2 -terminal domain of human CAR (CAR D1) were overexpressed in Escherichia coli as soluble protein fragments. (sciencemag.org)
  • Excess Ad5 fiber protein or anti-CAR antibody inhibits the binding of Ad5 to human erythrocytes and cryo-electron microscopy shows attachment via the fiber protein of Ad5, leading to close juxtaposition with the erythrocyte membrane. (bloodjournal.org)
  • We previously noted a region of amino acid sequence homology between A-gliadin, a major alpha-gliadin component known to activate coeliac disease, and the early region E1b protein of human adenovirus serotype 12 (Ad12), an adenovirus isolated from the human intestinal tract. (bmj.com)
  • Additional studies documented that a region of A-gliadin that shares amino acid sequence homology with the adenovirus 12 E1b protein could be recognised as an antigenic determinant in active coeliac disease patients. (bmj.com)
  • The feasibility of using adenovirus-mediated human oligopeptide transporter (hPEPT1) gene transfer to achieve peptide drug delivery to the brain across the blood-brain barrier was tested by examining the accumulation of model peptides in a rat brain endothelial cell line (RBEC1) and rat brain after transduction with a recombinant adenovirus encoding hPEPT1-enhanced yellow fluorescent protein fusion gene (AdhPEPT1-EYFP). (aspetjournals.org)
  • Based on the Ad5 genome, we have generated three types of telomerase-specific replication-competent oncolytic adenoviruses: OBP-301 (Telomelysin), green fluorescent protein (GFP)-expressing OBP-401 (TelomeScan), and tumor suppressor p53-armed OBP-702. (medworm.com)
  • A simple bioinformatics strategy identified a candidate granzyme B cleavage site in the widely expressed BNIP-2 (BCL2/adenovirus E1B-19K protein-interacting protein 2). (portlandpress.com)
  • The human involucrin protein is a specific product of keratinocyte differentiation, which is selectively expressed in the suprabasal epidermal layers. (aacrjournals.org)
  • We have described a live recombinant adenovirus vaccine prototype that produces abundant HPV16 L1 protein from the adenovirus major late transcriptional unit and directs the assembly of HPV16 VLPs in tissue culture. (asm.org)
  • Adenovirus early region 4 open reading frame 4 (E4orf4) protein is a novel cell death factor that selectively induces p53-independent apoptosis in cancer cells, but not in normal human cells. (ovid.com)
  • Results An isoform of CAR with a terminal SIV motif and a unique PDZ-binding domain was expressed at high levels in human beta cells at the protein level. (jyu.fi)
  • Immunogold labelling and electron microscopic analysis confirmed that CAR-SIV was localised to dense-core (insulin) secretory granules in human islets, whereas no immunolabelling of the protein was detected on the secretory granules of adjacent exocrine cells. (jyu.fi)
  • one group was vaccinated with the recombinant adenovirus expressing the influenza virus H3 hemagglutinin (HA) protein, one group was vaccinated with the recombinant adenovirus expressing the nucleoprotein (NP), and one group was vaccinated with both recombinants in a mixture. (flu.org.cn)
  • The refined crystal structure of hexon, the major coat protein of adenovirus type 2, at 2.9 Å resolution. (microbiologyresearch.org)
  • Adenovirus serotype evolution is driven by illegitimate recombination in the hypervariable regions of the hexon protein. (microbiologyresearch.org)
  • Human CAR protein has a theoretical molecular weight of 40.0 kDa and is composed of 365 amino acids. (wikipedia.org)
  • Adenovirus protein regulation of viral genes has been studied in adenovirus type-5, or Ad5. (wikipedia.org)
  • With regards to cellular translation of the host cell, it has been concluded that adenovirus selectively inhibits cellular translation via displacement of MAP kinase-interacting serine/threonine-protein kinase 1 (Mnk1), an eukaryotic translation initiation factor. (wikipedia.org)
  • The adenovirus early region 1A (E1A) 289R protein is composed of 289 amino acids, with four conserved regions: CR1 (42-80), CR2 (115-137), CR3 (145-191), CR4 (240-289). (wikipedia.org)
  • Adenoviruses commonly infect humans, causing colds, flu-like symptoms and sometimes even death, but now UC San Francisco researchers have discovered that a new species of adenovirus can spread from primate to primate, and potentially from monkey to human. (healthcanal.com)
  • SUBJECTS: Ninety four faecal samples were collected from three species of non-human primates consisting of various ages and sex. (biomedsearch.com)
  • These findings suggest that cross species transmission in Kenyan non-human primates may be a common occurrence and there is a possibility of zoonotic transmission of adenoviruses. (biomedsearch.com)
  • Depending on the epitopes used human ELISA kits can be cross reactive to many other species. (gentaur.com)
  • Whereas this observation strictly concerns parenteral delivery routes, orally-delivered vaccines are also sought, owing to their ease of delivery in humans and other species and their potential to elicit mucosal immunity. (frontiersin.org)
  • Species-specific identification of human adenoviruses by a multiplex PCR assay. (semanticscholar.org)
  • A multiplex PCR assay was developed by using primers to the fiber gene that could differentiate human adenovirus (Ad) species A through F in a single amplification reaction. (semanticscholar.org)
  • Respiratory disease caused by a species B2 adenovirus in a military camp in Turkey. (semanticscholar.org)
  • Furthermore, our results highlight the potential of using these non-human primates as models for testing safety and efficacy of candidate adenovirus vaccines prior to clinical trials in humans. (biomedsearch.com)
  • Live oral adenovirus vaccines have properties that are well-suited for use in such settings. (asm.org)
  • The induction of a strong cellular response by the recombinant indicates that live adenovirus recombinants have potential as vaccines for those agents. (asm.org)
  • These studies encourage and will inform the continued development of viable recombinant adenovirus vaccines. (asm.org)
  • Live adenovirus vaccines have been used by the United States military for decades to prevent adenovirus type 4 (Ad4)- and Ad7-induced severe upper respiratory disease in recruits ( 1 ). (asm.org)
  • In this study, two replication-defective adenovirus recombinants were developed as potential vaccines against H3N2 influenza viruses. (flu.org.cn)
  • Thus, replication-incompetent adenovirus vaccines given simultaneously to pigs are efficacious for SIV and have the additional advantage over commercial vaccines that suckling piglets have no pre-existing maternally-derived antibody to block early life vaccination. (flu.org.cn)
  • Adenovirus vaccines are live-virus vaccines approved for one-time use in new military members, ages 17 to 50 years. (health.mil)
  • Adenoviruses vaccine types 4 and 7 can be administered simultaneously or at any interval before or after other vaccines, including live vaccines. (health.mil)
  • However, when cells were infected with mutant viruses which did not express the larger E1B proteins (Ad12 dl620 and in602 and Ad5 dl338 and pm381) the concentration of p53 increased markedly to levels comparable to those seen in adenovirus transformed cells. (nih.gov)
  • Finally, Western blots using antibodies that inhibit virus binding to either the human or mouse CVB receptors detected 46-kDa proteins in HCAR- and MCAR-transfected cells, respectively. (pnas.org)
  • When such cotransduced cells reach an area of extreme hypoxia, the E1A/B proteins are expressed, thereby activating replication of the adenovirus. (aacrjournals.org)
  • Properties Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. (gentaur.com)
  • An arginine-glycine-aspartic acid amino acid motif is present in most but not all adenovirus penton base proteins and mediates interaction with αv integrins, resulting in internalization. (diva-portal.org)
  • The immunization of macaques induced vigorous humoral responses to adenovirus capsid and nonstructural proteins, although, surprisingly, not against HPV L1. (asm.org)
  • To explore the applicability of the live rAd platform to other pathogens, we constructed replication-competent adenovirus recombinants that make novel use of the high-level gene expression characteristic of the adenovirus major late transcriptional unit (MLTU) to produce papillomavirus L1 proteins ( 4 ). (asm.org)
  • Analysis of 15 adenovirus hexon proteins reveals the location and structure of seven hypervariable regions containing serotype-specific residues. (microbiologyresearch.org)
  • GENTAUR suppliers human normal cells, cell lines, RNA extracts and lots of antibodies and ELISA kits to Human proteins as well as PowerChekTM Human Adenovirus_Bocavirus_IPC Real_time RT_PCR Kit. (antibody-antibodies.com)
  • Adenovirus early region 1A (E1A) is a gene expressed during adenovirus replication to produce a variety of E1A proteins. (wikipedia.org)
  • These proteins are functionally important for growth of adenoviruses. (wikipedia.org)
  • The adenovirus early region 1A gene is translated to produce E1A proteins by using the host cell's molecular machinery. (wikipedia.org)
  • The E1A gene refers to the gene that encodes E1A proteins specifically involved in adenovirus replication. (wikipedia.org)
  • In the adenovirus, the final translation products, or E1A proteins, are proline-rich and have been found to localize in the nucleus. (wikipedia.org)
  • Except for two patients with clear increases in serum adenovirus antibody titers, no adverse tissue responses or systemic complications were noticed in any of the patients. (nih.gov)
  • We have re-evaluated the apoptotic potentials of seven human PC cell lines using the natural Fas ligand (FasL) in place of agonistic antibody. (nih.gov)
  • That the host cell nucleus is the principal site of adenovirus synthesis has been confirmed by fluorescent antibody studies. (rupress.org)
  • Atkinson RL, Lee I, Shin H-J, He J. Human adenovirus-36 antibody status is associated with obesity in children. (medigraphic.com)
  • The results showed that pigs in the groups given the recombinant adenovirus expressing HA alone and HA plus NP developed high levels of virus-specific hemagglutination-inhibition (HI) antibody by 4 weeks post vaccination. (flu.org.cn)
  • Large-scale studies of the new vaccine in U.S. military recruits showed high efficacy rates in preventing wild type 4 adenovirus-associated febrile acute respiratory disease and inducing neutralizing antibody to type 7 adenovirus. (health.mil)
  • Since 1953, 67 new types of human adenoviruses have been isolated, the collaborators said, adding that genome recombination is crucial in the molecular evolution of the viruses as new strains emerge and old ones re-emerge but change or are more virulent. (genomeweb.com)
  • The E2B region of the adenovirus genome encodes for DNA polymerase ( pol ) that is required for adenoviral DNA replication. (arvojournals.org)
  • These data indicate that, if the carcinogenic potential of adenovirus types 7, 12, and 18 lies in the possession of nucleotide sequences common to these three viruses, these regions represent a small portion of the total genome, approximately one to three viral cistrons. (sciencemag.org)
  • Five genome sequences of subspecies B1 human adenoviruses associated with acute respiratory disease. (harvard.edu)
  • The uncoupling between high-number genome copies of adenoviruses detected by real-time PCR and the absence of infectivity detected by tissue culture suggests that genome-based detection methods are inadequate for direct assessment of human health risk. (asm.org)
  • Porcine cell lines supported Ad5 replication, showing virus titers, kinetics of virus production, and luciferase expression levels similar to those obtained in human 293 cells, which constitutively express the 5'-end 11% of the Ad5 genome. (asm.org)
  • Hybridization of labelled cellular RNA with restriction fragments of adenovirus type 5 DNA indicated transcription of a portion of the adenovirus genome at the conventional left hand end. (microbiologyresearch.org)
  • Packaging cells having no overlapping sequences with a selected vector are suited for large-scale production of recombinant adenoviruses. (freepatentsonline.com)
  • Live recombinant adenoviruses (rAds) used in a similar manner might prove to be powerful tools for immunization against other pathogens, especially in low-resource settings. (asm.org)
  • Whereas specific viral genes are present and expressed in tumors induced by agents such as Epstein-Barr virus and the human papillomaviruses, no specific HCMV DNA sequence element is reliably present and often no viral DNA can be detected in cells transformed by HCMV (reviewed in refs. (pnas.org)
  • The real-time PCR methods showed a greater sensitivity in detection of adenoviruses in sewage samples than the viral plaque assay and in detection of enterococci in coastal waters than the bacterial culture method. (asm.org)
  • Among human viral pathogens, adenovirus is the only DNA virus in the enteric virus family, hence, the most thermostable virus, and can survive for prolonged periods in environmental waters ( 13 , 16 , 17 ). (asm.org)
  • 33 ) suggested that adenoviruses be used as an index of human viral pollution because of the concomitancy of adenovirus with other human viruses. (asm.org)
  • Proper monitoring of human viruses in our waters is of particular importance, because the Centers for Disease Control and Prevention suggest that the causative agent of nearly 50% of all acute gastrointestinal illnesses is suspected to be viral ( 6 ). (asm.org)
  • However, certain human viruses and coliphages may beneficially serve as an index in determining viral contamination and the presence of human fecal waste ( 20 , 31 , 32 ). (asm.org)
  • Adenovirus is frequently found in urban rivers and polluted coastal waters ( 5 , 7 , 20 , 32 , 33 , 39 ), and studies conducted in Europe suggest that adenovirus be used as an index of human viral pollution ( 32 ). (asm.org)
  • Siden adenovirus (Ad) RC er områder hvor viral genom replikering og uttrykk av virale sene gener finner sted, gir de et miljø for rekruttering av alle nødvendige viral og cellulære faktorer som deltar i disse prosessene. (jove.com)
  • During viral replication, adenovirus early region 1A (E1A) is a gene that is expressed. (wikipedia.org)
  • While adenovirus likely blocks cellular translation in this way, it does not affect the translation of its own viral mRNAs. (wikipedia.org)
  • This study raises more concerns about the potential of unknown viruses to spread from animals to humans," said Chiu, who is an assistant professor of medicine at UCSF. (healthcanal.com)
  • We still don't understand the full extent of viruses that exist in the world and their potential to cause outbreaks in human populations. (healthcanal.com)
  • In light of these findings, however, he said the normal vigilance in tracking animal viruses that might also infect humans should extend beyond influenza and coronaviruses to include adenoviruses. (healthcanal.com)
  • Pathogenic bacteria and enteric viruses can be introduced into the environment via human waste discharge. (asm.org)
  • Methods for rapid detection and quantification of human viruses and fecal indicator bacteria in water are urgently needed to prevent human exposure to pathogens through drinking and recreational waters. (asm.org)
  • Enveloped viruses, such as human immunodeficiency virus (HIV), attach to host cells by means of spike-like membrane glycoproteins, whereas most nonenveloped viruses, such as poliovirus, attach by means of specialized domains integral to their capsids. (sciencemag.org)
  • Although a few recent investigations have reported developing real-time PCR methods for quantification of human viruses and their detection in sewage ( 26 , 28 , 29 ), little is known yet about the quantitative abundance of human viruses in the aquatic environment ( 13 ). (asm.org)
  • With approximately 100 potentially water-transmissible human viruses associated with human waste, it is simply impossible to detect all viruses ( 2 ). (asm.org)
  • Adenoviruses are non-enveloped, double stranded DNA viruses that vary in size from 70 to 100 nm [ 1 ]. (plos.org)
  • Adenoviruses are medium-sized, nonenveloped icosohedral viruses containing double-stranded DNA. (health.mil)
  • BGI, George Mason University, the Massachusetts Eye and Ear Infirmary, and the University of Oklahoma Health Sciences Center announced they have signed the agreement, which aims to identify the molecular basis of adenovirus evolution. (genomeweb.com)
  • Mesenchymal stromal cells for linked delivery of oncolytic and apoptotic adenoviruses to non-small-cell lung cancers," Molecular Therapy , vol. 23, pp. 1497-1506, 2015. (hindawi.com)
  • Adenoviruses have been detected in raw sewage in relatively high frequencies ( 22 , 25 ), and they were found in environmental sources, including Southern California coastal waters, using molecular methods ( 23 , 33 ). (asm.org)
  • To determine the molecular basis for the specificity and tight affinity of this interaction ( 3 ), we crystallized and determined the structure of the Ad12 knob alone and in complex with human CAR D1 to 2.6 Å resolution ( 4 ). (sciencemag.org)
  • First molecular evidence for the existence of distinct fish and snake adenoviruses. (microbiologyresearch.org)
  • Adenovirus-induced endothelial cell activation was shown by VCAM-1 expression on virus-treated, cultured endothelial cells and by the release of ultra-large molecular weight multimers of VWF within 1 to 2 hours of virus administration with an accompanying elevation of endothelial microparticles. (bloodjournal.org)
  • BACKGROUND: Adenoviruses are known to cause several human diseases including acute febrile respiratory syndromes, epidemic conjunctivitis and gastroenteritis. (biomedsearch.com)
  • Adenovirus, a double-stranded DNA virus belonging to the family Adenoviridae , is a possible candidate as an index virus. (asm.org)
  • The adenovirus genetic information is encoded by a double stranded linear DNA molecule. (wikipedia.org)
  • Enhanced expression of p53 in human cells infected with mutant adenoviruses. (nih.gov)
  • The expression of p53 in human cells infected with wild-type (wt) and mutant adenoviruses has been examined. (nih.gov)
  • Upon transfection of salivary glands, adenovirus encoding human aquaporin-1 (AdhAQP1) directs human aquaporin-1 (hAQP1) expression in the apical and basolateral plasma membranes of salivary secretory cells, which may result in increased saliva production. (cancer.gov)
  • Human mesenchymal stem cells lack tumor tropism but enhance the antitumor activity of oncolytic adenoviruses in orthotopic lung and breast tumors," Human Gene Therapy , vol. 18, pp. 627-641, 2007. (hindawi.com)
  • Influence of carrier cells on the clinical outcome of children with neuroblastoma treated with high dose of oncolytic adenovirus delivered in mesenchymal stem cells," Cancer Letters , vol. 371, pp. 161-170, 2016. (hindawi.com)
  • Disruption of the antiproliferative TGF-beta signaling pathways in human pancreatic cancer cells," Oncogene , vol. 17, pp. 1969-1978, 1998. (hindawi.com)
  • Strikingly, AdmTyr-βgal is extremely active in human melanoma cells, in some cases exceeding the transcriptional activity of a cytomegalovirus promoter-driven recombinant β-gal virus. (aacrjournals.org)
  • Tissue specificity of gene expression is maintained, with very low levels observed in tumors and primary human cells derived from other lineages. (aacrjournals.org)
  • Chiu concluded that staff members had been exposed to the new virus, and that the virus may have jumped from baboon to human, an idea also supported by follow-up experiments in which laboratory strains of simian adenovirus C efficiently infected both human and baboon cells. (healthcanal.com)
  • Human 293 cells were cultured in Minimum Essential Medium (Eagle) with 2 mM L-glutamine and harvested at the log phase of growth. (bio-medicine.org)
  • In this approach, macrophages were cotransduced with a hypoxia-regulated E1A/B construct and an E1A-dependent oncolytic adenovirus, whose proliferation is restricted to prostate tumor cells using prostate-specific promoter elements from the TARP, PSA, and PMSA genes. (aacrjournals.org)
  • This novel delivery system employs 3 levels of tumor specificity: the natural "homing" of macrophages to hypoxic tumor areas, hypoxia-induced proliferation of the therapeutic adenovirus in host macrophages, and targeted replication of oncolytic virus in prostate tumor cells. (aacrjournals.org)
  • Here we show that the HCMV immediate early 1 and 2 genes can cooperate with the adenovirus E1A gene to generate transformed foci of primary baby rat kidney cells. (pnas.org)
  • HCMV has been shown to induce cervical neoplasia in mice ( 6 ), it can morphologically transform human ( 7 , 8 ) as well as other mammalian ( 9 - 11 ) cells in culture, and HCMV-transformed cells can form tumors in experimental animals ( 6 , 8 ). (pnas.org)
  • BRK cells, which have been used extensively to assay the transforming activity of adenovirus oncogenes ( 21 , 22 ), were used to test the oncogenic potential of the HCMV immediate early genes. (pnas.org)
  • Hyperplasia suppressor gene (HSG), also called human mitofusin 2, is a novel gene that markedly suppresses the cell proliferation of hyperproliferative vascular smooth muscle cells from spontaneously hypertensive rat arteries. (aacrjournals.org)
  • The sequential cytological changes which develop in tissue culture cells infected with adenovirus types 5 and 7 are described and compared with those produced by adenovirus types 1, 2, 3, and 4. (rupress.org)
  • The human adenovirus type 19a/64 (hAd19a) is a rare serotype in the human population that transduces human dendritic cells (DCs) and human muscle cells more efficiently than the well-characterized human adenovirus type 5 (hAd5). (uni-muenchen.de)
  • We determined the ability of Ad-36 to increase glucose uptake by human primary skeletal muscle (HSKM) cells. (diabetesjournals.org)
  • The following experiments showed that Ad-36 activates PI 3-kinase and increases glucose uptake in nondiabetic and diabetic human skeletal muscle (HSKM) cells. (diabetesjournals.org)
  • Furthermore, target cells in which BID (BH3-interacting domain death agonist) expression was inhibited also remained highly susceptible to NK cell-mediated killing, revealing redundancy in the pro-apoptotic response to human cytotoxic lymphocytes. (portlandpress.com)
  • Oncolytic adenoviruses act by replicating within and lysing tumor cells. (aacrjournals.org)
  • MTT assay was applied to measure the lethal effects of virus on pancreatic cancer cells, and ELISA was adopted to detect the human endostatin gene expression. (lu.se)
  • In contrast, EGF-E4orf4 showed no significant inhibitory effect iresn primary cells of human umbilical vein endothelial cells. (ovid.com)
  • In the present study, we have determined the profile of CAR isoforms present in human beta cells and monitored the subcellular localisation of the principal isoform within the cells. (jyu.fi)
  • Human embryonic kidney cells have been transformed by exposing cells to sheared fragments of adenovirus type 5 DNA. (microbiologyresearch.org)
  • Analysis of the polypeptides synthesized in the 293 cells by labelling with 35 S-methionine and SDS PAGE showed a variable pattern of synthesis, different in a number of respects from that seen in other human cells. (microbiologyresearch.org)
  • Remarkably, MAV-1 displayed a higher affinity for primary human smooth muscle cells than recombinant Ad5 (rAd5). (surrey.ac.uk)
  • Attachment of MAV-1 to human and mouse cells of hepatocyte origin was not altered by physiological concentrations of human coagulation factor XI (FXI) or the vitamin K-dependent FIX, FX and FVII. (surrey.ac.uk)
  • 2012) Human adenovirus-specific T cells modulate HIV-specific T cell responses to an Ad5-vectored HIV-1 vaccine. (oalib.com)
  • Adenovirus likely displaces Mnk1 from eIF4G and inhibits eIF4E phosphorylation, both of which are important components of the eIF4a translation initiation complex in cells. (wikipedia.org)
  • Acute gastroenteritis causes illness and death in humans worldwide. (cdc.gov)
  • Presented are ways to address the problem of replication competent adenovirus in adenoviral production for use with, for example, gene therapy. (freepatentsonline.com)
  • The feasibility of gene therapy for CAH was studied using 21OH-deficient mice (21OH−) and a replication-deficient adenovirus containing the genomic sequence of human CYP21 (hAdCYP21). (nature.com)
  • Both retro- and adenovirus-mediated gene therapy have been suggested as a novel approach to the treatment of malignant brain tumors. (nih.gov)
  • These preliminary in vivo results suggest a potential use for adenovirus encoding FasL as a gene therapy for PC. (nih.gov)
  • hi Fujiwara Adenovirus serotype 5 (Ad5) is widely and frequently used as a virus vector in cancer gene therapy and oncolytic virotherapy. (medworm.com)
  • Adenovirus-induced thrombocytopenia is a potentially serious complication of gene therapy protocols using this type of vector. (bloodjournal.org)
  • The murine tyrosinase promoter-enhancer expression cassette was introduced into the E1 region of a recombinant adenovirus to generate the virus AdmTyr-βgal. (aacrjournals.org)
  • In contrast to the human class I molecules analyzed, transport of the murine H-2Dd molecule was not inhibited in the presence of E19. (jimmunol.org)
  • Using surface plasmon resonance, MAV-1 was shown to directly associate with human FX and murine FX and FIX but, opposite to rAd5, this interaction did not lead to enhanced cellular attachment. (surrey.ac.uk)
  • All the faecal samples were screened for presence of adenoviruses using a commercial antigen-capture enzyme immunoassay (EIA) kit, this is an enzyme-linked immunosorbent assay (ELISA) kit designed for diagnosis of human enteric adenoviruses in stool samples. (biomedsearch.com)
  • First, PC cell lines were tested in a standard cytotoxicity assay with a transfected cell line that stably expresses human FasL. (nih.gov)
  • However, a plaque assay using two human tissue culture cell lines, HEK-293A and A549, yielded negative results, suggesting that adenoviruses detected by real-time PCR are likely noninfectious. (asm.org)
  • Duplex-PCR assay for the detection of adenovirus and respiratory syncytial virus in nasopharyngeal samples. (semanticscholar.org)
  • In order to assess the applicability of human adenoviruses to the evaluation of the faecal contamination in European bathing waters, a real-time quantitative PCR assay was developed for the quantification of human adenoviruses in 132 samples collected from 24 different recreational marine and freshwater sites in nine European countries. (ub.edu)
  • Immunohistochemical and in situ hybridisation detection of adenovirus early region 1A (E1A) gene in the microglia of human brain tissue. (bmj.com)
  • In this report, we showed that an adenovirus vector encoding human HSG (Ad5-hHSG) had an antitumor activity in a wide range of cancer cell lines. (aacrjournals.org)
  • A nonreplicating adenovirus vector encoding β-galactosidase (rAd-βgal) was administered intravenously to determine the relationship between circulating AdNAb titer and vector gene expression in the liver. (aacrjournals.org)
  • Therefore, we examined the effects of transferring the cyclin-dependent kinase inhibitor p21 sdi1 gene into human esophageal cancer cell lines as well as normal keratinocytes using an adenovirus vector system. (aacrjournals.org)
  • Can adenoviruses cause acute respiratory disease? (health.mil)
  • In the United States, acute respiratory disease is often associated with adenovirus Types 4, 7, and 14. (health.mil)
  • Human adenoviruses were first isolated in 1953 and were initially identified as respiratory pathogens. (genomeweb.com)
  • The prolonged viability of the naturally occurring E. faecalis under simulated deep-sea conditions is a concern with respect to the contamination of the deep ocean with human pathogens and the possibility of sewage-associated microorganisms ( 3 ). (asm.org)
  • Fluorescence Quantitative PCR was performed to detect adenovirus as well as other common ARI-related pathogens. (plos.org)
  • We describe a new cell-based method that selectively targets an oncolytic adenovirus to hypoxic areas of prostate tumors. (aacrjournals.org)
  • In this study, we show for the first time that macrophages can be engineered to silently transport to, and then produce high titres of therapeutic adenovirus specifically in hypoxic areas of experimental prostate tumors. (aacrjournals.org)
  • A variety of laboratory observations demonstrate that HCMV has oncogenic potential, supporting the idea that HCMV might contribute to the induction of human tumors. (pnas.org)
  • Transplantation of human erythrocytes into nonobese diabetic/severe combined immunodeficiency mice extends blood circulation of intravenous Ad5 but decreases its extravasation into human xenograft tumors. (bloodjournal.org)
  • The development of an improved HPV vaccine therefore remains a high priority and an attractive opportunity for assessing the utility of a replicating adenovirus vaccine. (asm.org)
  • Questions and answers about Adenoviruses and adenovirus vaccine. (health.mil)
  • Additionally, there is a U.S. Food and Drug Administration (FDA) approved/licensed vaccine for adenovirus types 4 and 7. (health.mil)
  • Adenovirus types 4 and 7 can be prevented with a single vaccine dose administered as two live, oral enteric-coated tablets (type 4 - white tablet, type 7- light peach tablet). (health.mil)
  • How effective is the adenovirus vaccine? (health.mil)
  • What are the components of the adenovirus vaccine? (health.mil)
  • The adenovirus vaccine contains viable, selected strains of human adenovirus Type 4 and human adenovirus Type 7 prepared in human-diploid fibroblast cell cultures (strain WI-38). (health.mil)
  • Adenovirus vaccine types 4 and 7 are tablets and are given orally. (health.mil)
  • How many doses of adenovirus vaccine are needed? (health.mil)
  • Adenovirus vaccine types 4 and 7 is administered only once and no booster dose is required. (health.mil)
  • The transgenic cell lines expressing pol-D36 and the fluorescent marker gene were obtained from human lung carcinoma cell lines A549, H1299 transduced with the corresponding recombinant lentiviruses. (arvojournals.org)
  • Serological detection of adenoviruses in non-human primates maintained in a colony in Kenya. (biomedsearch.com)
  • The prevalence of adenovirus was not significantly different between rainy and dry seasons. (uib.no)
  • We found similar prevalence of adenovirus in non-diarrhoeic children and in diarrhoeic children. (uib.no)
  • METHODS: In the present study, we assessed the tropism of mouse adenovirus type 1 (MAV-1), a nonhuman adenovirus for which cellular attachment is CAR-independent. (surrey.ac.uk)
  • Lastly, adenoviral-mediated expression of FasL prevented growth and induced regression of two human PC cell lines in immunodeficient mice. (nih.gov)
  • The quantification of human adenoviruses may provide complementary information in relation to the use of bacterial standards in the control of water quality in bathing water. (ub.edu)
  • and adenoviruses, titer from 3 x 10(8) to 3 x 10(10) PFU) were injected into the tumor via the catheter once a day for three consecutive days, followed by tumor resection 1-2 days later. (nih.gov)
  • Initially, we showed that human macrophages adenovirally infected with a HIF-regulated reporter or a therapeutic gene and then cocultured with human tumor spheroids in vitro , migrated into the hypoxic centre of these structures and expressed the exogenous gene ( 11 ). (aacrjournals.org)
  • We have previously reported ( 13 ) that the two most abundantly expressed immediate-early gene products of HCMV, IE1 and IE2 (reviewed in ref. 14 ), inhibit the induction of apoptosis by tumor necrosis factor α (TNF-α) or the adenovirus E1A oncoproteins. (pnas.org)
  • A xenograft tumor-bearing nude mouse model was developed to assess how a similar in vivo titer would impact the activity of 01/PEME, an oncolytic adenovirus, after intravenous administration. (aacrjournals.org)
  • To this end, a human tumor xenograft nude mouse model was developed to model preexisting humoral immunity to adenovirus observed in the human population. (aacrjournals.org)
  • A human tumor xenograft nude mouse model was developed to evaluate the inhibitory effect of human AdNAbs on the antitumor efficacy of an oncolytic adenovirus administered by the intravenous route. (aacrjournals.org)
  • The purpose of this study was to assess the impact of anti-adenovirus neutralizing antibodies (AdNAbs) on the distribution, tolerability, and efficacy of intravenously administered oncolytic adenovirus. (aacrjournals.org)
  • Type 5 adenovirus (Ad5) is a human pathogen that has been widely developed for therapeutic uses, with only limited success to date. (bloodjournal.org)
  • Recombinant Human Adenovirus Type 5, an E1B gene deleted adenovirus, is known to have a significant antitumor activity. (clinicaltrials.gov)
  • A similar titer reconstituted in the nude mice with human serum, as was done in the SCID/beige mice, did not abrogate the antitumor efficacy of the replicating adenovirus after intravenous administration. (aacrjournals.org)
  • In this study, we developed a model to test whether or not preexisting humoral immunity representative of that found in the general population would be sufficient to neutralize the antitumor efficacy of a replicating oncolytic adenovirus. (aacrjournals.org)
  • We wished to model the effect of preexisting humoral immunity to adenovirus on antitumor efficacy of an oncolytic adenovirus. (aacrjournals.org)
  • To increase the efficacy and decrease the side-effect, we constructed a conditionally replicative adenovirus (CRAd) expressing human endostatin, with a human Telomoerase Reverse Transcriptase (hTERT) promoter for the regulation of the early stage of adenovirus expression of gene E1a and a Hypoxia Response Element (HRE) promoter to regulate the gene E1b. (lu.se)
  • In 2009, scientists demonstrated that a more virulent strain of human adenovirus arose from recombination with other distinct strains of milder human adenoviruses. (healthcanal.com)
  • Otherwise, the overlapping sequences would enable homologous recombination leading to replication competent adenovirus in the primary cell into which the isolated recombinant nucleic acid molecule is to be transferred. (freepatentsonline.com)
  • Methods: A gene recombination technique was adopted to construct and generate the adenovirus AdTPHre-hEndo. (lu.se)
  • Construction and characterization of hexon-chimeric adenoviruses: specification of adenovirus serotype. (microbiologyresearch.org)
  • Hybridization measurements among the DNA's of six weakly tumorigenic strains of human adenovirus type 7 reveal that all six strains are very closely related (83- to 110-percent nucleotide sequence homology). (sciencemag.org)
  • Human adenovirus 36 induces adiposity, increases insulin sensitivity, and alters hypothalamic monoamines in rats. (medigraphic.com)
  • Adenovirus-mediated Overexpression of p15INK4B Inhibits Human Glioma Cell Growth, Induces Replicative Senescence, and Inhibits Telomerase Activity Similarly to p16INK4A -- Fuxe et al. (aacrjournals.org)
  • Therefore, modeling the effect of preexisting adenovirus immunity on systemic administration of therapeutic adenoviruses is challenging. (aacrjournals.org)
  • Not for any clinical, therapeutic, or diagnostic use in humans or animals. (nordicbiosite.com)
  • This new virus likely formed when an existing adenovirus recombined with another, generating a new strain that was highly virulent to baboons. (healthcanal.com)
  • Initially, severe combined immunodeficient (SCID)/beige mice were passively immunized with various amounts of human sera to establish a condition of preexisting humoral immunity similar to humans. (aacrjournals.org)
  • As a first step in model development, severe combined immunodeficient (SCID)/beige mice were passively immunized with different pools of human sera to establish a condition of preexisting immunity. (aacrjournals.org)
  • Metagenomic analysis showed that adenovirus sequences that were closely to the A31 and A61 genotypes were the most abundant. (springer.com)