Species of the genus MASTADENOVIRUS, causing a wide range of diseases in humans. Infections are mostly asymptomatic, but can be associated with diseases of the respiratory, ocular, and gastrointestinal systems. Serotypes (named with Arabic numbers) have been grouped into species designated Human adenovirus A-F.
A family of non-enveloped viruses infecting mammals (MASTADENOVIRUS) and birds (AVIADENOVIRUS) or both (ATADENOVIRUS). Infections may be asymptomatic or result in a variety of diseases.
Respiratory and conjunctival infections caused by 33 identified serotypes of human adenoviruses.
Proteins transcribed from the E1A genome region of ADENOVIRUSES which are involved in positive regulation of transcription of the early genes of host infection.
Virus diseases caused by the ADENOVIRIDAE.
Proteins encoded by adenoviruses that are synthesized prior to, and in the absence of, viral DNA replication. The proteins are involved in both positive and negative regulation of expression in viral and cellular genes, and also affect the stability of viral mRNA. Some are also involved in oncogenic transformation.
Proteins transcribed from the E1B region of ADENOVIRUSES which are involved in regulation of the levels of early and late viral gene expression.
Proteins transcribed from the E3 region of ADENOVIRUSES but not essential for viral replication. The E3 19K protein mediates adenovirus persistence by reducing the expression of class I major histocompatibility complex antigens on the surface of infected cells.
Proteins transcribed from the E4 region of ADENOVIRUSES. The E4 19K protein transactivates transcription of the adenovirus E2F protein and complexes with it.
The very first viral gene products synthesized after cells are infected with adenovirus. The E1 region of the genome has been divided into two major transcriptional units, E1A and E1B, each expressing proteins of the same name (ADENOVIRUS E1A PROTEINS and ADENOVIRUS E1B PROTEINS).
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
Species of the genus MASTADENOVIRUS that causes fever, edema, vomiting, and diarrhea in dogs and encephalitis in foxes. Epizootics have also been caused in bears, wolves, coyotes, and skunks. The official species name is Canine adenovirus and it contains two serotypes.
Proteins transcribed from the E2 region of ADENOVIRUSES. Several of these are required for viral DNA replication.
A genus of ADENOVIRIDAE that infects MAMMALS including humans and causes a wide range of diseases. The type species is Human adenovirus C (see ADENOVIRUSES, HUMAN).
Species of the genus MASTADENOVIRUS, causing neurological disease in pigs.
A genus of ADENOVIRIDAE that infects birds. The type species is FOWL ADENOVIRUS A.
Techniques and strategies which include the use of coding sequences and other conventional or radical means to transform or modify cells for the purpose of treating or reversing disease conditions.
The type species of the genus AVIADENOVIRUS, family ADENOVIRIDAE, an oncogenic virus of birds. This is also called CELO virus for chick embryo lethal orphan virus.
Deoxyribonucleic acid that makes up the genetic material of viruses.
The introduction of functional (usually cloned) GENES into cells. A variety of techniques and naturally occurring processes are used for the gene transfer such as cell hybridization, LIPOSOMES or microcell-mediated gene transfer, ELECTROPORATION, chromosome-mediated gene transfer, TRANSFECTION, and GENETIC TRANSDUCTION. Gene transfer may result in genetically transformed cells and individual organisms.
Proteins that form the CAPSID of VIRUSES.
The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
Established cell cultures that have the potential to propagate indefinitely.
The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.
Specific molecular components of the cell capable of recognizing and interacting with a virus, and which, after binding it, are capable of generating some signal that initiates the chain of events leading to the biological response.
Use of attenuated VIRUSES as ANTINEOPLASTIC AGENTS to selectively kill CANCER cells.
Tumor-selective, replication competent VIRUSES that have antineoplastic effects. This is achieved by producing cytotoxicity-enhancing proteins and/or eliciting an antitumor immune response. They are genetically engineered so that they can replicate in CANCER cells but not in normal cells, and are used in ONCOLYTIC VIROTHERAPY.
Simultaneous inflammation of the cornea and conjunctiva.
The transfer of bacterial DNA by phages from an infected bacterium to another bacterium. This also refers to the transfer of genes into eukaryotic cells by viruses. This naturally occurring process is routinely employed as a GENE TRANSFER TECHNIQUE.
Proteins found in any species of virus.
The functional hereditary units of VIRUSES.
Products of viral oncogenes, most commonly retroviral oncogenes. They usually have transforming and often protein kinase activities.
Inflammation, often mild, of the conjunctiva caused by a variety of viral agents. Conjunctival involvement may be part of a systemic infection.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Vaccines used to prevent infection by any virus from the family ADENOVIRIDAE.
The outer protein protective shell of a virus, which protects the viral nucleic acid.
An inheritable change in cells manifested by changes in cell division and growth and alterations in cell surface properties. It is induced by infection with a transforming virus.
Genes that are introduced into an organism using GENE TRANSFER TECHNIQUES.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Visible morphologic changes in cells infected with viruses. It includes shutdown of cellular RNA and protein synthesis, cell fusion, release of lysosomal enzymes, changes in cell membrane permeability, diffuse changes in intracellular structures, presence of viral inclusion bodies, and chromosomal aberrations. It excludes malignant transformation, which is CELL TRANSFORMATION, VIRAL. Viral cytopathogenic effects provide a valuable method for identifying and classifying the infecting viruses.
Viruses which enable defective viruses to replicate or to form a protein coat by complementing the missing gene function of the defective (satellite) virus. Helper and satellite may be of the same or different genus.
Viruses which lack a complete genome so that they cannot completely replicate or cannot form a protein coat. Some are host-dependent defectives, meaning they can replicate only in cell systems which provide the particular genetic function which they lack. Others, called SATELLITE VIRUSES, are able to replicate only when their genetic defect is complemented by a helper virus.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.
Ribonucleic acid that makes up the genetic material of viruses.
Any of the processes by which cytoplasmic factors influence the differential control of gene action in viruses.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
A genus of the family PICORNAVIRIDAE whose members preferentially inhabit the intestinal tract of a variety of hosts. The genus contains many species. Newly described members of human enteroviruses are assigned continuous numbers with the species designated "human enterovirus".
The process by which a DNA molecule is duplicated.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
A genus of ADENOVIRIDAE that comprises viruses of several species of MAMMALS and BIRDS. The type species is Ovine adenovirus D.
A species of POLYOMAVIRUS originally isolated from Rhesus monkey kidney tissue. It produces malignancy in human and newborn hamster kidney cell cultures.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
Mutant mice homozygous for the recessive gene "nude" which fail to develop a thymus. They are useful in tumor studies and studies on immune responses.
Process of growing viruses in live animals, plants, or cultured cells.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
The complete genetic complement contained in a DNA or RNA molecule in a virus.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
A cell line derived from cultured tumor cells.
Invasion of the host RESPIRATORY SYSTEM by microorganisms, usually leading to pathological processes or diseases.
Production of new arrangements of DNA by various mechanisms such as assortment and segregation, CROSSING OVER; GENE CONVERSION; GENETIC TRANSFORMATION; GENETIC CONJUGATION; GENETIC TRANSDUCTION; or mixed infection of viruses.
Immunoglobulins produced in response to VIRAL ANTIGENS.
Minute infectious agents whose genomes are composed of DNA or RNA, but not both. They are characterized by a lack of independent metabolism and the inability to replicate outside living host cells.
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Those proteins recognized by antibodies from serum of animals bearing tumors induced by viruses; these proteins are presumably coded for by the nucleic acids of the same viruses that caused the neoplastic transformation.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
Process of determining and distinguishing species of bacteria or viruses based on antigens they share.
An area showing altered staining behavior in the nucleus or cytoplasm of a virus-infected cell. Some inclusion bodies represent "virus factories" in which viral nucleic acid or protein is being synthesized; others are merely artifacts of fixation and staining. One example, Negri bodies, are found in the cytoplasm or processes of nerve cells in animals that have died from rabies.
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
Inflammation of the lung parenchyma that is caused by a viral infection.
A suborder of PRIMATES consisting of six families: CEBIDAE (some New World monkeys), ATELIDAE (some New World monkeys), CERCOPITHECIDAE (Old World monkeys), HYLOBATIDAE (gibbons and siamangs), CALLITRICHINAE (marmosets and tamarins), and HOMINIDAE (humans and great apes).
Substances elaborated by viruses that have antigenic activity.
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
A ubiquitously expressed complement receptor that binds COMPLEMENT C3B and COMPLEMENT C4B and serves as a cofactor for their inactivation. CD46 also interacts with a wide variety of pathogens and mediates immune response.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Eukaryotic cell line obtained in a quiescent or stationary phase which undergoes conversion to a state of unregulated growth in culture, resembling an in vitro tumor. It occurs spontaneously or through interaction with viruses, oncogenes, radiation, or drugs/chemicals.
A genus of the family PARVOVIRIDAE, subfamily PARVOVIRINAE, which are dependent on a coinfection with helper adenoviruses or herpesviruses for their efficient replication. The type species is Adeno-associated virus 2.
Suspensions of attenuated or killed viruses administered for the prevention or treatment of infectious viral disease.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
INFLAMMATION of any segment of the GASTROINTESTINAL TRACT from ESOPHAGUS to RECTUM. Causes of gastroenteritis are many including genetic, infection, HYPERSENSITIVITY, drug effects, and CANCER.
This line KB is now known to be a subline of the ubiquitous KERATIN-forming tumor cell line HeLa. It was originally thought to be derived from an epidermal carcinoma of the mouth, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via contamination by HELA CELLS. The cells are positive for keratin by immunoperoxidase staining. KB cells have been reported to contain human papillomavirus18 (HPV-18) sequences.
Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected.
Viruses that produce tumors.
The directional growth of an organism in response to an external stimulus such as light, touch, or gravity. Growth towards the stimulus is a positive tropism; growth away from the stimulus is a negative tropism. (From Concise Dictionary of Biology, 1990)
Injections introduced directly into localized lesions.
An enzyme that catalyzes the conversion of ATP and thymidine to ADP and thymidine 5'-phosphate. Deoxyuridine can also act as an acceptor and dGTP as a donor. (From Enzyme Nomenclature, 1992) EC
In vivo methods of screening investigative anticancer drugs, biologic response modifiers or radiotherapies. Human tumor tissue or cells are transplanted into mice or rats followed by tumor treatment regimens. A variety of outcomes are monitored to assess antitumor effectiveness.
Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH.
The genetic unit consisting of three structural genes, an operator and a regulatory gene. The regulatory gene controls the synthesis of the three structural genes: BETA-GALACTOSIDASE and beta-galactoside permease (involved with the metabolism of lactose), and beta-thiogalactoside acetyltransferase.
A subfamily of the family MURIDAE comprised of 69 genera. New World mice and rats are included in this subfamily.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
Proteins prepared by recombinant DNA technology.
The genetic process of crossbreeding between genetically dissimilar parents to produce a hybrid.
The measurement of infection-blocking titer of ANTISERA by testing a series of dilutions for a given virus-antiserum interaction end-point, which is generally the dilution at which tissue cultures inoculated with the serum-virus mixtures demonstrate cytopathology (CPE) or the dilution at which 50% of test animals injected with serum-virus mixtures show infectivity (ID50) or die (LD50).
Small synthetic peptides that mimic surface antigens of pathogens and are immunogenic, or vaccines manufactured with the aid of recombinant DNA techniques. The latter vaccines may also be whole viruses whose nucleic acids have been modified.
Cell changes manifested by escape from control mechanisms, increased growth potential, alterations in the cell surface, karyotypic abnormalities, morphological and biochemical deviations from the norm, and other attributes conferring the ability to invade, metastasize, and kill.
Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Tumors or cancer of the MOUTH.
Elements of limited time intervals, contributing to particular results or situations.
Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.
A general term for diseases produced by viruses.
Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
Transcription factors that were originally identified as site-specific DNA-binding proteins essential for DNA REPLICATION by ADENOVIRUSES. They play important roles in MAMMARY GLAND function and development.
Experimentally induced new abnormal growth of TISSUES in animals to provide models for studying human neoplasms.
Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
Defective viruses which can multiply only by association with a helper virus which complements the defective gene. Satellite viruses may be associated with certain plant viruses, animal viruses, or bacteriophages. They differ from satellite RNA; (RNA, SATELLITE) in that satellite viruses encode their own coat protein.
The sum of the weight of all the atoms in a molecule.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
Nuclear phosphoprotein encoded by the p53 gene (GENES, P53) whose normal function is to control CELL PROLIFERATION and APOPTOSIS. A mutant or absent p53 protein has been found in LEUKEMIA; OSTEOSARCOMA; LUNG CANCER; and COLORECTAL CANCER.
The infective system of a virus, composed of the viral genome, a protein core, and a protein coat called a capsid, which may be naked or enclosed in a lipoprotein envelope called the peplos.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
Y-box-binding protein 1 was originally identified as a DNA-binding protein that interacts with Y-box PROMOTER REGIONS of MHC CLASS II GENES. It is a highly conserved transcription factor that regulates expression of a wide variety of GENES.
An alpha integrin with a molecular weight of 160-kDa that is found in a variety of cell types. It undergoes posttranslational cleavage into a heavy and a light chain that are connected by disulfide bonds. Integrin alphaV can combine with several different beta subunits to form heterodimers that generally bind to RGD sequence-containing extracellular matrix proteins.
Genes whose expression is easily detectable and therefore used to study promoter activity at many positions in a target genome. In recombinant DNA technology, these genes may be attached to a promoter region of interest.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Proteins which are involved in the phenomenon of light emission in living systems. Included are the "enzymatic" and "non-enzymatic" types of system with or without the presence of oxygen or co-factors.
Deoxycytidine (dihydrogen phosphate). A deoxycytosine nucleotide containing one phosphate group esterified to the deoxyribose moiety in the 2'-,3'- or 5- positions.
Enzymes that oxidize certain LUMINESCENT AGENTS to emit light (PHYSICAL LUMINESCENCE). The luciferases from different organisms have evolved differently so have different structures and substrates.
Method for measuring viral infectivity and multiplication in CULTURED CELLS. Clear lysed areas or plaques develop as the VIRAL PARTICLES are released from the infected cells during incubation. With some VIRUSES, the cells are killed by a cytopathic effect; with others, the infected cells are not killed but can be detected by their hemadsorptive ability. Sometimes the plaque cells contain VIRAL ANTIGENS which can be measured by IMMUNOFLUORESCENCE.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.
The binding of virus particles to receptors on the host cell surface. For enveloped viruses, the virion ligand is usually a surface glycoprotein as is the cellular receptor. For non-enveloped viruses, the virus CAPSID serves as the ligand.
Experimental transplantation of neoplasms in laboratory animals for research purposes.
A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.
Product of the retinoblastoma tumor suppressor gene. It is a nuclear phosphoprotein hypothesized to normally act as an inhibitor of cell proliferation. Rb protein is absent in retinoblastoma cell lines. It also has been shown to form complexes with the adenovirus E1A protein, the SV40 T antigen, and the human papilloma virus E7 protein.
The study of the structure, growth, function, genetics, and reproduction of viruses, and VIRUS DISEASES.
A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.
Vaccines or candidate vaccines containing inactivated HIV or some of its component antigens and designed to prevent or treat AIDS. Some vaccines containing antigens are recombinantly produced.
An ACYCLOVIR analog that is a potent inhibitor of the Herpesvirus family including cytomegalovirus. Ganciclovir is used to treat complications from AIDS-associated cytomegalovirus infections.
The relationships of groups of organisms as reflected by their genetic makeup.
Infections of the eye caused by minute intracellular agents. These infections may lead to severe inflammation in various parts of the eye - conjunctiva, iris, eyelids, etc. Several viruses have been identified as the causative agents. Among these are Herpesvirus, Adenovirus, Poxvirus, and Myxovirus.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
Processes that stimulate the GENETIC TRANSCRIPTION of a gene or set of genes.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
The rate dynamics in chemical or physical systems.
The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990)
Receptors such as INTEGRIN ALPHAVBETA3 that bind VITRONECTIN with high affinity and play a role in cell migration. They also bind FIBRINOGEN; VON WILLEBRAND FACTOR; osteopontin; and THROMBOSPONDINS.
A genus of ADENOVIRIDAE comprising species including viruses of frogs (FROGS AND TOADS) and TURKEYS. The type species is Frog adenovirus.
Directed modification of the gene complement of a living organism by such techniques as altering the DNA, substituting genetic material by means of a virus, transplanting whole nuclei, transplanting cell hybrids, etc.
The integration of exogenous DNA into the genome of an organism at sites where its expression can be suitably controlled. This integration occurs as a result of homologous recombination.
An enzyme that catalyzes the acetylation of chloramphenicol to yield chloramphenicol 3-acetate. Since chloramphenicol 3-acetate does not bind to bacterial ribosomes and is not an inhibitor of peptidyltransferase, the enzyme is responsible for the naturally occurring chloramphenicol resistance in bacteria. The enzyme, for which variants are known, is found in both gram-negative and gram-positive bacteria. EC
Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
A malignant neoplasm made up of epithelial cells tending to infiltrate the surrounding tissues and give rise to metastases. It is a histological type of neoplasm but is often wrongly used as a synonym for "cancer." (From Dorland, 27th ed)
Any immunization following a primary immunization and involving exposure to the same or a closely related antigen.
Macromolecular molds for the synthesis of complementary macromolecules, as in DNA REPLICATION; GENETIC TRANSCRIPTION of DNA to RNA, and GENETIC TRANSLATION of RNA into POLYPEPTIDES.
A member of the p300-CBP transcription factors that was originally identified as a binding partner for ADENOVIRUS E1A PROTEINS.
Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.
A sequence of successive nucleotide triplets that are read as CODONS specifying AMINO ACIDS and begin with an INITIATOR CODON and end with a stop codon (CODON, TERMINATOR).
Polyomavirus antigens which cause infection and cellular transformation. The large T antigen is necessary for the initiation of viral DNA synthesis, repression of transcription of the early region and is responsible in conjunction with the middle T antigen for the transformation of primary cells. Small T antigen is necessary for the completion of the productive infection cycle.
CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). The species is a favorite for cytogenetic studies because of its small chromosome number. The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells.
Inactivation of viruses by non-immune related techniques. They include extremes of pH, HEAT treatment, ultraviolet radiation, IONIZING RADIATION; DESICCATION; ANTISEPTICS; DISINFECTANTS; organic solvents, and DETERGENTS.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.
A fractionated cell extract that maintains a biological function. A subcellular fraction isolated by ultracentrifugation or other separation techniques must first be isolated so that a process can be studied free from all of the complex side reactions that occur in a cell. The cell-free system is therefore widely used in cell biology. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p166)
A species of CERCOPITHECUS containing three subspecies: C. tantalus, C. pygerythrus, and C. sabeus. They are found in the forests and savannah of Africa. The African green monkey (C. pygerythrus) is the natural host of SIMIAN IMMUNODEFICIENCY VIRUS and is used in AIDS research.
Forceful administration into a muscle of liquid medication, nutrient, or other fluid through a hollow needle piercing the muscle and any tissue covering it.
Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.
A critical subpopulation of regulatory T-lymphocytes involved in MHC Class I-restricted interactions. They include both cytotoxic T-lymphocytes (T-LYMPHOCYTES, CYTOTOXIC) and CD8+ suppressor T-lymphocytes.
A genus of the family HERPESVIRIDAE, subfamily BETAHERPESVIRINAE, infecting the salivary glands, liver, spleen, lungs, eyes, and other organs, in which they produce characteristically enlarged cells with intranuclear inclusions. Infection with Cytomegalovirus is also seen as an opportunistic infection in AIDS.
The assembly of VIRAL STRUCTURAL PROTEINS and nucleic acid (VIRAL DNA or VIRAL RNA) to form a VIRUS PARTICLE.
New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms.
Proteins conjugated with deoxyribonucleic acids (DNA) or specific DNA.
Bacteriophage and type species in the genus Tectivirus, family TECTIVIRIDAE. They are specific for Gram-negative bacteria.
A genus of the family HERPESVIRIDAE, subfamily ALPHAHERPESVIRINAE, consisting of herpes simplex-like viruses. The type species is HERPESVIRUS 1, HUMAN.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
Antibodies that reduce or abolish some biological activity of a soluble antigen or infectious agent, usually a virus.
A species of ENTEROVIRUS infecting humans and containing 36 serotypes. It is comprised of all the echoviruses and a few coxsackieviruses, including all of those previously named coxsackievirus B.
Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).
The production of PEPTIDES or PROTEINS by the constituents of a living organism. The biosynthesis of proteins on RIBOSOMES following an RNA template is termed translation (TRANSLATION, GENETIC). There are other, non-ribosomal peptide biosynthesis (PEPTIDE BIOSYNTHESIS, NUCLEIC ACID-INDEPENDENT) mechanisms carried out by PEPTIDE SYNTHASES and PEPTIDYLTRANSFERASES. Further modifications of peptide chains yield functional peptide and protein molecules.
Proteins found mainly in icosahedral DNA and RNA viruses. They consist of proteins directly associated with the nucleic acid inside the NUCLEOCAPSID.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
The entering of cells by viruses following VIRUS ATTACHMENT. This is achieved by ENDOCYTOSIS, by direct MEMBRANE FUSION of the viral membrane with the CELL MEMBRANE, or by translocation of the whole virus across the cell membrane.
Vaccines or candidate vaccines used to prevent EBOLA HEMORRHAGIC FEVER.
Immunized T-lymphocytes which can directly destroy appropriate target cells. These cytotoxic lymphocytes may be generated in vitro in mixed lymphocyte cultures (MLC), in vivo during a graft-versus-host (GVH) reaction, or after immunization with an allograft, tumor cell or virally transformed or chemically modified target cell. The lytic phenomenon is sometimes referred to as cell-mediated lympholysis (CML). These CD8-positive cells are distinct from NATURAL KILLER CELLS and NATURAL KILLER T-CELLS. There are two effector phenotypes: TC1 and TC2.
Cis-acting DNA sequences which can increase transcription of genes. Enhancers can usually function in either orientation and at various distances from a promoter.
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
The top portion of the pharynx situated posterior to the nose and superior to the SOFT PALATE. The nasopharynx is the posterior extension of the nasal cavities and has a respiratory function.
Tumor suppressor genes located on the short arm of human chromosome 17 and coding for the phosphoprotein p53.
A conserved A-T rich sequence which is contained in promoters for RNA polymerase II. The segment is seven base pairs long and the nucleotides most commonly found are TATAAAA.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
A family of basic helix-loop-helix transcription factors that control expression of a variety of GENES involved in CELL CYCLE regulation. E2F transcription factors typically form heterodimeric complexes with TRANSCRIPTION FACTOR DP1 or transcription factor DP2, and they have N-terminal DNA binding and dimerization domains. E2F transcription factors can act as mediators of transcriptional repression or transcriptional activation.

CAR-dependent and CAR-independent pathways of adenovirus vector-mediated gene transfer and expression in human fibroblasts. (1/2914)

Primary fibroblasts are not efficiently transduced by subgroup C adenovirus (Ad) vectors because they express low levels of the high-affinity Coxsackie virus and adenovirus receptor (CAR). In the present study, we have used primary human dermal fibroblasts as a model to explore strategies by which Ad vectors can be designed to enter cells deficient in CAR. Using an Ad vector expressing the human CAR cDNA (AdCAR) at high multiplicity of infection, primary fibroblasts were converted from being CAR deficient to CAR sufficient. Efficiency of subsequent gene transfer by standard Ad5-based vectors and Ad5-based vectors with alterations in penton and fiber was evaluated. Marked enhancement of binding and transgene expression by standard Ad5 vectors was achieved in CAR-sufficient fibroblasts. Expression by AdDeltaRGDbetagal, an Ad5-based vector lacking the arginine-glycine-aspartate (RGD) alphaV integrin recognition site from its penton base, was achieved in CAR-sufficient, but not CAR-deficient, cells. Fiber-altered Ad5-based vectors, including (a) AdF(pK7)betagal (bearing seven lysines on the end of fiber) (b) AdF(RGD)betagal (bearing a high-affinity RGD sequence on the end of fiber), and (c) AdF9sK betagal (bearing a short fiber and Ad9 knob), demonstrated enhanced gene transfer in CAR-deficient fibroblasts, with no further enhancement in CAR-sufficient fibroblasts. Together, these observations demonstrate that CAR deficiency on Ad targets can be circumvented either by supplying CAR or by modifying the Ad fiber to bind to other cell-surface receptors.  (+info)

Reduced phosphorylation of p50 is responsible for diminished NF-kappaB binding to the major histocompatibility complex class I enhancer in adenovirus type 12-transformed cells. (2/2914)

Reduced cell surface levels of major histocompatibility complex class I antigens enable adenovirus type 12 (Ad12)-transformed cells to escape immunosurveillance by cytotoxic T lymphocytes (CTL), contributing to their tumorigenic potential. In contrast, nontumorigenic Ad5-transformed cells harbor significant cell surface levels of class I antigens and are susceptible to CTL lysis. Ad12 E1A mediates down-regulation of class I transcription by increasing COUP-TF repressor binding and decreasing NF-kappaB activator binding to the class I enhancer. The mechanism underlying the decreased binding of nuclear NF-kappaB in Ad12-transformed cells was investigated. Electrophoretic mobility shift assay analysis of hybrid NF-kappaB dimers reconstituted from denatured and renatured p50 and p65 subunits from Ad12- and Ad5-transformed cell nuclear extracts demonstrated that p50, and not p65, is responsible for the decreased ability of NF-kappaB to bind to DNA in Ad12-transformed cells. Hypophosphorylation of p50 was found to correlate with restricted binding of NF-kappaB to DNA in Ad12-transformed cells. The importance of phosphorylation of p50 for NF-kappaB binding was further demonstrated by showing that an NF-kappaB dimer composed of p65 and alkaline phosphatase-treated p50 from Ad5-transformed cell nuclear extracts could not bind to DNA. These results suggest that phosphorylation of p50 is a key step in the nuclear regulation of NF-kappaB in adenovirus-transformed cells.  (+info)

Microtubule-dependent plus- and minus end-directed motilities are competing processes for nuclear targeting of adenovirus. (3/2914)

Adenovirus (Ad) enters target cells by receptor-mediated endocytosis, escapes to the cytosol, and then delivers its DNA genome into the nucleus. Here we analyzed the trafficking of fluorophore-tagged viruses in HeLa and TC7 cells by time-lapse microscopy. Our results show that native or taxol-stabilized microtubules (MTs) support alternating minus- and plus end-directed movements of cytosolic virus with elementary speeds up to 2.6 micrometer/s. No directed movement was observed in nocodazole-treated cells. Switching between plus- and minus end-directed elementary speeds at frequencies up to 1 Hz was observed in the periphery and near the MT organizing center (MTOC) after recovery from nocodazole treatment. MT-dependent motilities allowed virus accumulation near the MTOC at population speeds of 1-10 micrometer/min, depending on the cell type. Overexpression of p50/dynamitin, which is known to affect dynein-dependent minus end-directed vesicular transport, significantly reduced the extent and the frequency of minus end-directed migration of cytosolic virus, and increased the frequency, but not the extent of plus end-directed motility. The data imply that a single cytosolic Ad particle engages with two types of MT-dependent motor activities, the minus end- directed cytoplasmic dynein and an unknown plus end- directed activity.  (+info)

Differences in the interactions of oncogenic adenovirus 12 early region 1A and nononcogenic adenovirus 2 early region 1A with the cellular coactivators p300 and CBP. (4/2914)

Association with the cellular coactivators p300 and CBP is required for the growth-regulatory function of adenoviral (Ad) early region 1A (E1A) proteins. E1A regions necessary for these interactions overlap with domains involved in the induction of tumours in immunocompetent rodents through highly oncogenic Ad12. Differences in the association of cellular factors with the respective E1A domains of Ad12 and nononcogenic Ad2 might therefore be involved in serotype-specific oncogenicity. We analyzed the interaction of the Ad12 E1A 235R protein with p300 and CBP. Here we demonstrate that in the case of Ad12, but not Ad2/5, amino acids (aa) 1-29 of E1A proteins are sufficient to bind the p300-C/H3 domain in vivo and wild-type p300 in vitro. The conserved arginine-2, which is essential for the interaction between Ad2 E1A and p300, was dispensable for the Ad12 E1A 235R-p300 interaction in vitro. In addition to the p300-C/H3 region, we identified a second domain within p300 (aa 1999-2200) binding to the 235R protein. Contrary to p300, the amino-terminus and CR1 are necessary to associate with CBP. The aa 1-29 of the 235R protein but not CR1 are essential for the repression of colTRE-driven gene expression. This repression function is strictly dependent on p300 but not on CBP.  (+info)

Evidence for an adenovirus type 2-coded early glycoprotein. (5/2914)

We have identified an adenovirus type 2 (Ad2)-induced early glycopolypeptide with an apparent molecular weight of 20,000 to 21,000 (20/21K), as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The 20/21K polypeptide could be labeled in vivo with [(3)H]glucosamine. [(35)S]methionine- and [(3)H]-glucosamine-labeled 20/21K polypeptides bound to concanavalin A-Sepharose columns and were eluted with 0.2 M methyl-alpha-d-mannoside. The pulse-labeled polypeptide appeared as a sharp band with an apparent molecular weight of 21K, but after a chase it converted to multiple bands with an average molecular weight of 20K. This variability in electrophoretic mobility is consistent with glycosylation or deglycosylation of the 20/21K polypeptide. Analysis of the pulse and pulse-chase-labeled forms by using partial proteolysis indicated that the polypeptides were highly related chemically, but not identical. Most of the 20/21K polypeptide is localized in the cytoplasm fraction of infected cells lysed by Nonidet P-40. The 20/21K polypeptide and a 44K polypeptide, labeled with [(35)S]methionine or [(3)H]glucosamine in Ad2-infected human cells, were precipitated by a rat antiserum against an Ad2-transformed rat cell line (T2C4), but not by antisera against three other Ad2-transformed rat cell lines, or by serum from nonimmune rats. The partial proteolysis patterns of the 20/21K and the 44K polypeptides were indistinguishable, indicating that the two polypeptides are highly related, and suggesting that the 44K polypeptide might be a dimer of the 20/21K polypeptide. The 20/21K polypeptide was also induced in Ad2-early infected monkey and hamster cells. These results imply that the 20/21K polypeptide is synthesized in Ad2-infected human, monkey, and hamster cells, and in one but not all Ad2-transformed rat cells. Thus, the 20/21K polypeptide is probably viral coded rather than cell coded and viral induced.  (+info)

Development and use of a 293 cell line expressing lac repressor for the rescue of recombinant adenoviruses expressing high levels of rabies virus glycoprotein. (6/2914)

An expression cassette designed for high-level production of rabies virus glycoprotein (RG) could not be rescued into a replication-defective, adenovirus-based vector using standard procedures. To overcome this difficulty, a 293-based cell line, designated 293LAP13, was constructed that contained and expressed a derivative of the lac repressor protein. The lac operator sequence, to which the repressor binds, was incorporated into an expression cassette, containing a promoter and intron, designed for high-level production of RG. Insertion of a single operator sequence immediately downstream of the transcription start site and the use of the 293LAP13 cell line allowed recombinant viruses that could not be isolated with 293 cells to be rescued efficiently. The operator-containing virus reached higher titres in 293LAP13 than in parental 293 cells and also produced plaques more efficiently in 293LAP13 cells. Moreover, in non-complementing human and canine cell lines, adenovirus vectors with a promoter-intron expression cassette expressed RG at much higher levels than vectors lacking the intron. These observations, together with the demonstration that expression of RG by operator-containing vectors was repressed markedly in 293LAP13 cells and that this inhibition was relieved at least partly by IPTG, suggest that the 293LAP13 cell line may be useful for the rescue and propagation of many vectors in which high expression of the desired protein prevents vector rescue in 293 cells.  (+info)

The adenoviral E1A oncoproteins interfere with the growth-inhibiting effect of the cdk-inhibitor p21(CIP1/WAF1). (7/2914)

The cdk-inhibitor p21(CIP1/WAF1) inhibits the activities of cyclin-dependent kinases and proliferating cell nuclear antigen, thereby repressing cell-cycle progression and DNA replication. Transforming oncogenes, such as E1A of human adenovirus 5 (Ad5), may interfere with such growth-inhibitory proteins. In this study, we show that in various Ad5E1-transformed cells, p21(CIP1/WAF1) is expressed and that, in general, expression is not downregulated. In addition, colony-formation assays show that in Ad5E1-transformed cells highly overexpressed p21(CIP1/WAF1) can still cause growth inhibition. FACS experiments indicate, however, that a G1 arrest induced by moderate overexpression of p21(CIP1/WAF1) can be overcome by E1A. The E1A proteins may interfere with the function of p21(CIP1/WAF1) by binding. Indeed, p21(CIP1/WAF1) binds with its cyclin/cdk-binding N terminus to the transforming N-terminal and CR1 region of the E1A proteins. Together, these results lend support to the model that E1A can interfere directly with p21(CIP1/WAF1) function and thereby stimulates cell growth.  (+info)

Early region 1 transforming functions are dispensable for mammary tumorigenesis by human adenovirus type 9. (8/2914)

Some human adenoviruses are tumorigenic in rodents. Subgroup A and B human adenoviruses generally induce sarcomas in both male and female animals, and the gene products encoded within viral early region 1 (E1 region) are both necessary and sufficient for this tumorigenicity. In contrast, subgroup D human adenovirus type 9 (Ad9) induces estrogen-dependent mammary tumors in female rats and requires the E4 region-encoded ORF1 oncoprotein for its tumorigenicity. Considering the established importance of the viral E1 region for tumorigenesis by adenoviruses, we investigated whether this viral transcription unit is also necessary for Ad9 to generate mammary tumors. The nucleotide sequence of the Ad9 E1 region indicated that the gene organization and predicted E1A and E1B polypeptides of Ad9 are closely related to those of other human adenovirus E1 regions. In addition, an Ad9 E1 region plasmid demonstrated focus-forming activity in both low-passage-number and established rat embryo fibroblasts, whereas a large deletion within either the E1A or E1B gene of this plasmid diminished transforming activity. Surprisingly, we found that introducing the same transformation-inactivating E1A and E1B deletions into Ad9 results in mutant viruses that retain the ability to elicit mammary tumors in rats. These results are novel in showing that Ad9 represents a unique oncogenic adenovirus in which the E4 region, rather than the E1 region, encodes the major oncogenic determinant in the rat.  (+info)

Transarterial chemoembolization (TACE) is currently one of the mainstays of palliative treatments worldwide for patients with unresectable Hepatocellular Carcinoma(HCC).However, the long term outcomes were generally poor for HCC patients treated with TACE. Recombinant Human Adenovirus Type 5, an E1B gene deleted adenovirus, is known to have a significant antitumor activity. In addition, local injection of recombinant human adenovirus type 5 can enhance the effect of antitumor therapies (chemotherapy and radiotherapy). The hypothesis is that patients with unresectable HCC may benefit from recombinant human adenovirus type 5 in combination with TACE ...
Summary Evidence is presented here which indicates that the adenovirus DNA-binding protein (DBP) is phosphorylated at a tyrosine residue early in infection. This was suggested by the discovery that a proportion of the label in 32P-labelled DBP was resistant to alkali, and was substantiated by acid hydrolysis of DBP immunoprecipitates and by immunoblotting with a monoclonal antibody against phosphotyrosine. Treatment of [35S] methionine-labelled DBPs with chymotrypsin produced fragments of apparent M r 45K and 39K whereas digestion of 32P-labelled DBP resulted in fragments of 45K and 26K. Consideration of the distribution of 32P label and its alkali stability in these fragments suggested that chymotrypsin cleaved populations of DBP at different sites depending on their phosphorylation states. The conservation, in all of the seven adenovirus serotypes sequenced, of a tyrosine residue (at amino acid 195 in adenovirus type 2) together with its surrounding residues, suggests that phosphorylation
TY - JOUR. T1 - Adenovirus transcription. III. Mapping of viral RNA sequences in cells productively infected by adenovirus type 5. AU - Flint, S. J.. AU - Berget, S. M.. AU - Sharp, Phillip A.. N1 - Funding Information: We thank Jan Haverty for technical assistance and R. J. Roberts for a generous gift of endonuclease Barn HI. S. J. Flint was supported by a fellowship from the Science Research Council of Great Britain, and S. M. Berget by a fellowship from N.I.H. P. A. Sharp is supported by an American Cancer Society faculty grant. This work was funded by grants from the National Cancer Institute (No. CA 13106-03) and the American Cancer Society (No. VC-151).. PY - 1976/7/15. Y1 - 1976/7/15. N2 - Separated strands of restriction endonuclease Eco R1 and Hpa 1 fragments of 32P-labeled Adenovirus 5 DNA have been used in saturation hybridization experiments with cytoplasmic RNA extracted from human cells infected with Adenovirus type 5. The results of such experiments have allowed the construction ...
Human adenoviruses are non-enveloped dsDNA viruses of almost 35 kb in size [1]. HAdV can infect a variety of tissues and cause a wide range of complications like gastroenteritis, hepatitis, myocarditis, keratoconjunctivitis and pneumonia [2, 3]. It is contagion in nature which occurs through direct contact or fomites and virus is also resistant to various physical and chemical agents. Children younger than the age of 5 years and immune compromised persons especially the pediatric patients are most susceptible to these viruses. Worldwide 5-7% respiratory tract infections are ascribed by HAdV in pediatric patients [4] and persons of all ages are susceptible to infections caused by these viruses [5].. Seven known Human adenoviruses species from HAdV-A to HAdV-G are constitute of the genus Mastadenovirus in which all the human adenoviruses are categorized and further divided into different strains [6]. Now 67 types of HAdV have been reported [7]. Their number is rapidly increasing due to ...
Fingerprint Dive into the research topics of Accumulation of early and intermediate mRNA species during subgroup C adenovirus productive infections. Together they form a unique fingerprint. ...
In 2005, a human adenovirus strain (formerly known as HAdV-D22/H8 but renamed here HAdV-D53) was isolated from an outbreak of epidemic keratoconjunctititis (EKC), a disease that is usually caused by HAdV-D8, -D19, or -D37, not HAdV-D22. To date, a complete change of tropism compared to the prototype has never been observed, although apparent recombinant strains of other viruses from species Human adenovirus D (HAdV-D) have been described. The complete genome of HAdV-D53 was sequenced to elucidate recombination events that lead to the emergence of a viable and highly virulent virus with a modified tropism. Bioinformatic and phylogenetic analyses of this genome demonstrate that this adenovirus is a recombinant of HAdV-D8 (including the fiber gene encoding the primary cellular receptor binding site), HAdV-D22, (the ε determinant of the hexon gene), HAdV-D37 (including the penton base gene encoding the secondary cellular receptor binding site), and at least one unknown or unsequenced HAdV-D strain.
In 2005, a human adenovirus strain (formerly known as HAdV-D22/H8 but renamed here HAdV-D53) was isolated from an outbreak of epidemic keratoconjunctititis (EKC), a disease that is usually caused by HAdV-D8, -D19, or -D37, not HAdV-D22. To date, a complete change of tropism compared to the prototype has never been observed, although apparent recombinant strains of other viruses from species Human adenovirus D (HAdV-D) have been described. The complete genome of HAdV-D53 was sequenced to elucidate recombination events that lead to the emergence of a viable and highly virulent virus with a modified tropism. Bioinformatic and phylogenetic analyses of this genome demonstrate that this adenovirus is a recombinant of HAdV-D8 (including the fiber gene encoding the primary cellular receptor binding site), HAdV-D22, (the ε determinant of the hexon gene), HAdV-D37 (including the penton base gene encoding the secondary cellular receptor binding site), and at least one unknown or unsequenced HAdV-D strain.
Entry of adenoviruses into the host cell involves two sets of interactions between the virus and the host cell. Most of the action occurs at the vertices. Entry into the host cell is initiated by the knob domain of the fiber protein binding to the cell receptor. The two currently established receptors are: CD46 for the group B human adenovirus serotypes and the coxsackievirus adenovirus receptor (CAR) for all other serotypes. There are some reports suggesting MHC molecules and sialic acid residues functioning in this capacity as well. This is followed by a secondary interaction, where a motif in the penton base protein interacts with an integrin molecule. It is the co-receptor interaction that stimulates entry of the adenovirus. This co-receptor molecule is αv integrin. Binding to αv integrin results in endocytosis of the virus particle via clathrin-coated pits. Attachment to αv integrin stimulates cell signaling and thus induces actin polymerization resulting in entry of the virion into the ...
Background Human Adenoviruses (HAdVs) cause a wide array of illnesses in all age groups. They particularly cause frequent morbidity among children. In China, human adenovirus types 3, 4, 7, 11, 14, 21, and 55 have caused at least seven outbreaks since 2000. However, limited st...
Adenovirus genomes are linear, non-segmented double-stranded (ds) DNA molecules that are typically 26-46 Kbp long, containing 23-46 protein-coding genes. The example used for the following description is Human adenovirus E, a mastadenovirus with a 36 Kbp genome containing 38 protein-coding genes. While the precise number and identity of genes varies among adenoviruses, the basic principles of genome organization and the functions of most of the genes described in this article are shared among all adenoviruses. The 38 genes in the Human Adenovirus E genome are organized in 17 transcription units, each containing 1-8 coding sequences. Alternative splicing during processing of the pre-mRNAs produced by each transcription unit enable multiple different mRNAs to be produced from one transcription unit. The E1A, E1B, E2A, E2B, E3, and E4 transcription units are successively transcribed early in the viral reproductive cycle. The proteins coded for by genes within these transcription units are mostly ...
Vectors derived from human adenovirus type 5, which typically lack the E1A and E1B genes, induce robust innate immune responses that limit their therapeutic efficacy. mutant-infected cells in the absence or presence of exogenous IFN. Both the concentration of viral genomes detected during the late phase and the numbers of viral replication centers formed were strongly reduced in IFN-treated cells in the absence of the E1B protein, despite production of similar quantities of viral replication proteins. These defects could not be attributed to degradation of entering viral genomes, induction of apoptosis, or failure to reorganize components of PML nuclear bodies. Nor was assembly of Nutlin 3a the E1B- and E4 Orf6 protein- E3 ubiquitin ligase required to prevent inhibition of viral replication by IFN. However, by using RT-PCR, the E1B 55 kDa protein was demonstrated to be a potent repressor of expression of IFN-inducible genes in IFN-treated cells. We propose that a primary function of the ...
To define the bottlenecks that restrict antigen expression after oral administration of viral-vectored vaccines, we tracked vectors derived from the human adenovirus type 5 at whole body, tissue and cellular scales throughout the digestive tract in a murine model of oral delivery. After intragastric administration of vectors encoding firefly luciferase or a model antigen, detectable levels of transgene-encoded protein or mRNA were confined to the intestine, and restricted to delimited anatomical zones. Expression of luciferase in the form of multiple small bioluminescent foci in the distal ileum, cecum and proximal colon suggested multiple crossing points. Many foci were unassociated with visible Peyers patches, implying that transduced cells lay in proximity to villous rather than follicle-associated epithelium, as supported by detection of transgene-encoded antigen in villous epithelial cells. Transgene-encoded mRNA but not protein was readily detected in Peyers patches, suggesting that post
Human adenovirus (HAdV) is a common pathogen causing a broad spectrum of diseases. HAdV encodes the pVII protein, which is involved in nuclear delivery, protection and expression of viral DNA. To suppress the cellular interferon (IFN) and RNA interference (RNAi) systems, HAdVs encode non-coding virus-associated (VA) RNAs. In this thesis we have investigated the functional significance of the pVII protein and VA RNAI in HAdV-5 infected cells.. We report that the propeptide module is the destabilizing element targeting the precursor pVII protein for proteasomal degradation. We also found that the Cul3-based E3 ubiquitin ligase complex alter the precursor pVII protein stability via binding to the propeptide sequence. In addition, we show that inhibition of the Cul3 protein reduces HAdV-5 E1A gene expression. Collectively, our results suggest a novel function for the pVII propeptide module and involvement of Cul3 in viral E1A gene expression.. Our studies show that the cellular E3 ubiquitin ligase ...
Human adenovirus 12 ATCC ® VR-863D™ Designation: DNA from Human adenovirus 12 strain Huie [ATCC ® VR-863™] Application: It is suitable for use in polymerase chain reaction (PCR) for viral gene products and other molecular virology applications. Respiratory research
Human adenovirus 2 ATCC ® VR-846D™ Designation: DNA from Human adenovirus 2 strain Adenoid 6 [ATCC ® VR-846™] Application: It is suitable for use in polymerase chain reaction (PCR) for viral gene products and other molecular virology applications. Respiratory research
It has been known for some time that the human adenovirus serotype 5 (Ad5) E4orf6 and Sox18 E1B55K proteins work in concert to degrade p53 and to regulate selective export of late viral mRNAs during productive infection. H1299 human lung carcinoma cells after expression of E1B55K and E4orf6 using adenovirus vectors. Several species were detected and identified by mass spectroscopy and for one of these integrin α3 we went on in a parallel study to confirm it as a substrate of the complex (F. Dallaire et al. J. Virol. 83:5329-5338 2009 Although the system has some limitations it may still be of some general use in identifying candidate substrates of any viral cullin-based E3 ubiquitin ligase complex and we suggest a series of criteria for substrate validation. During the past decade protein degradation has become increasingly recognized SVT-40776 (Tarafenacin) as a critical mechanism by which cells regulate a number of fundamental processes (reviewed in references 37 57 and 59). Degradation ...
Adenoviral nucleic acid was detected by polymerase chain reaction (PCR) in pharyngeal and rectal swab samples of a cat seropositive for adenovirus and suffering from transient hepatic failure. The samples were taken at a one-year interval, and both faecal samples as well as the second pharyngeal sample were positive in ... read more PCR performed with general adenovirus primers. The size of the amplified products corresponded to that of the positive control. The identity of the amplicons was also confirmed by DNA sequencing. The 301 bp long hexon gene fragment was very similar to but distinguishable from the corresponding hexon sequence of human adenovirus type 2. This result suggests the possibility of persistent carrier status and shedding of adenovirus in cats. show less ...
Antisera against hexons of serotypes 2, 4, 5, and 6 (subgroup III), and 15 (subgroup II) were absorbed with purified hexons of various serotypes representing the different subgroups of human adenoviruses. Group, subgroup, and type specificities of hexons could be distinguished. The subgroup specificity of type 4 hexons resembled that of hexons of subgroup I members (types 3, 11, and 16). Antihexon sera gave a type-specific inhibition of virion-associated hemagglutinin. The inhibiting activity of different sera was found to be inversely related to the length of fibers of the serotype concerned. Virions of serotypes carrying fibers shorter than about 20 nm (types 3, 4, 9, 11, and 15) were readily inhibited, whereas those of serotypes with longer fibers (types 2 and 6) were inhibited only by relatively large amounts of antibody measured in terms of homotypic complement fixation activity. The reciprocal cross-neutralization between serotypes 4 and 16 was studied separately. Hexons of both serotypes ...
The precursor of the 55K adenovirus terminal protein is an 87K protein that is covalently linked to viral DNA. This protein is likely to be identical to the 80,000 dalton protein described by Challberg et al. (1980). The mRNA for the 87K terminal protein precursor, like that for the E2-72K DNA binding protein, is detectable at both early and late times of infection, and its production is sensitive to protein synthesis inhibition (Lewis and Mathews, 1980). The 87K protein, together with proteins of 105,000 and 75,000 daltons, are translated from leftward transcribed (1-strand) messenger RNAs that are complementary to the viral genome between positions 11.2 and 31.5. Additional hybridization to the region between coordinates 37.3 and 41 suggests that the RNA body is spliced to sequences mapping farther right in the genome. Electron microscopic heteroduplex analysis has revealed a family of 1-strand RNAs that probably encode these proteins. The RNA bodies extend from coordinated 30, 26 and 23 to ...
Recombinant adenoviruses currently are used for a variety of purposes, including gene transfer in vitro, vaccination in vivo, and gene therapy (1-4). Several features of adenovirus biology have made such viruses the vectors of choice for certain of these applications. For example, adenoviruses transfer genes to a broad spectrum of cell types, and gene transfer is not dependent on active cell division. Additionally, high titers of viruses and high levels of transgene expression generally can be obtained.. Decades of study of adenovirus biology have resulted in a detailed picture of the viral life cycle and the functions of the majority of viral proteins (5, 6). The genome of the most commonly used human adenovirus (serotype 5) consists of a linear, 36-kb, double-stranded DNA molecule. Both strands are transcribed and nearly all transcripts are heavily spliced. Viral transcription units are conventionally referred to as early (E1, E2, E3, and E4) and late, depending on their temporal expression ...
The cryo-electron microscopy (cryo-EM) structure of the complex between the trimeric human adenovirus B serotype 3 fibre knob and human desmoglein 2 fragments containing cadherin domains EC2 and EC3 has been published, showing 3:1 and 3:2 complexes. Here, the crystal structure determined at 4.5 Å resolution is presented with one EC2-EC3 desmoglein fragment bound per fibre knob monomer in...
Adenovirus has been associated with both sporadic and epidemic disease and, with regard to infections among military recruits, who were routinely immunized against types 4 and 7 from 1971 until the cessation of vaccine production in 1996. Adenovirus became a significant cause of economic cost and morbidity in this setting. A live oral vaccine against adenovirus types 4 and 7 was approved for use in this population by the US Food and Drug Administration (FDA) in 2011, and subsequent incidence of acute respiratory disease declined.. Of interest is the role of adenoviruses as vectors in vaccination and in gene therapy. [1, 2, 3] Adenoviruses can infect various cells, both proliferating and quiescent, and thus hold the promise of targeting many different tissues and diseased cell lines.. The genome of adenovirus is well known and can be modified with relative ease to induce lysis or cytotoxicity of a specified cell line without affecting others.. The virus itself can be engineered to remove its ...
The E4 region of human adenovirus type 2 is predicted to encode seven proteins as judged from its nucleotide sequence and the pattern of differential splicing of its transcript. Two of the open reading frames (ORFs), ORF1 and ORF2, had been identified as being disrupted in the recently published sequence of the related serotype 5 virus. These ORFs were resequenced and found to be intact in the wt300 strain of adenovirus type 5.
Most adenoviruses bind directly to the coxsackie and adenovirus receptor (CAR) on target cells in vitro, but recent research has shown that adenoviruses can also use soluble components in body fluids for indirect binding to target cells. These mechanisms have been identified upon addressing the questions of how to de- and retarget adenovirus-based vectors for human gene and cancer therapy, but the newly identified mechanisms also suggest that the role of body fluids and their components may also be of importance for natural, primary infections. Here we demonstrate that plasma, saliva, and tear fluid promote binding and infection of adenovirus type 5 (Ad5) in respiratory and ocular epithelial cells, which corresponds to the natural tropism of most adenoviruses, and that plasma promotes infection by Ad31. By using a set of binding and infection experiments, we also found that Ad5 and Ad31 require coagulation factors IX (FIX) or X (FX) or just FIX, respectively, for efficient binding and infection. ...
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NEW YORK (GenomeWeb News) - Researchers from China and the US will sequence 100 human adenoviruses, including ones that cause respiratory, gastrointestinal, and ocular diseases, under a partnership announced today.
The quest for an efficacious HIV vaccine has resulted in several clinical trial failures, including the Step Trial, which used a replication-incompetent adenovirus (AdV) vector called human adenovirus type 5 (HAdV-5). Despite eliciting strong cellular immune responses, these trials were prematurely halted due to statistical futility resulting from increased HIV acquisition in vaccinated individuals. The Step Study showed increased HIV susceptibility in HAdV-5 baseline seropositive subjects, which complicates the use of HAdV-5 vectors since pre-existing neutralizing antibodies (nAb) to HAdV-5 are prevalent worldwide. Sampling the unique immunological phenotypes of the rectal mucosa - the site of HIV infection in the Step Study, and of AdV persistence and trafficking - could help explain this trial, since only peripheral blood (PBMC) was collected from subjects. We obtained rectal lamina propria T lymphocytes (rLPL) from a rhesus macaque (RM) model vaccinated with a species-specific simian Ad type 7 (SAdV
TY - JOUR. T1 - Repression of cytochrome P‐450c gene expression by cotransfection with adenovirus E1a DNA. AU - SOGAWA, Kazuhiro. AU - HANDA, Hiroshi. AU - FUJISAWA‐SEHARA, Atsuko. AU - HIROMASA, Takako. AU - YAMANE, Miyuki. AU - FUJII‐KURIYAMA, Yoshiaki. PY - 1989/5. Y1 - 1989/5. N2 - Gene expression of rat cytochrome P‐450c (P‐450c) depends upon inducible enhancers scattered in the 5′‐upstream region of the gene. We show that expression of the P‐450c gene is repressed by contransfection with adenovirus E1a DNA, regardless of the presence or absence of inducers, in a transient expression system of HeLa cells. Since cotransfection of either 13S or 12S E1a cDNA was effective in the repression, the region necessary for repression could be separated from that of transactivation of other adenovirus early genes. Moreover, we investigated the regions responsible for the inhibitory activity using in‐frame deletion mutants lacking internal or external portions of the E1a proteins. The ...
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SignaGen Laboratories, A Gene Delivery Company Providing Custom AAV Adenovirus Lentivirus Production Services & Manufacturing DNA/siRNA Transfection Reagents... Ad-GIT2 [SL100903] - Product Category: Human Adenovirus Type5 (dE1/E3) expressing G Protein-coupled Receptor Kinase Interactor 2 (GIT2) under a CMV promoter. Product Information Product Name: G Protein-coupled Receptor Kinase Interactor 2, pre-packaged adenovirus, ready to ship and ready to use format. Promoter: CMV Titer: 1E+10~1E+11 PFU/ml Storage Buffer: DMEM with 2.5% BSA, 2.5% glycerol Gene Information Gene Name: G
SignaGen Laboratories, A Gene Delivery Company Providing Custom AAV Adenovirus Lentivirus Production Services & Manufacturing DNA/siRNA Transfection Reagents... Ad-PRKD3 [SL100971] - Product Category: Human Adenovirus Type5 (dE1/E3) expressing Protein Kinase D3 (PRKD3). Product Information Product Name: Protein Kinase D3, pre-made adenovirus, ready to ship and ready to use format. Promoter: CMV Titer: 1E+10 ~ 1E+11 PFU/ml Storage Buffer: DMEM with 2.5% BSA, 2.5% glycerol Gene Information Gene Name: Protein Kinase D3 NCBI Acc. #: NM_005813 Gene Symbol: PRKD3 Gene ID:
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There are many stages in the development of a new drug for viral infection and such processes are even further complicated for adenovirus by the fact that there are at least 51 serotypes, forming six distinct groups (A-F), with different degree of infectivity. This review attempts to address the importance of developing pharmaceuticals for adenovirus and also review recent development in drug discovery for adenovirus, including newer strategies such as microRNA approaches. Different drug screening strategies will also be discussed.
Adenovirus Type 9, 0.1 mg. The many different serotypes of human adenoviruses (Ad) are divided into six subgroups, of which all Ad subgroup A and B and two subgroup D Ads can elicit tumors in infected rodents.
A HIV-1 DNA prime-recombinant Adenovirus Type 5 (rAd5) increase vaccine failed to guard against HIV-1 acquisition. is certainly towards the gp41 subunit from the envelope (Env) glycoprotein from the pathogen (1). This antibody response derives from polyreactive B cells that cross-react with Env and intestinal microbiota (IM) (2, 3). Nevertheless, it is unidentified if an identical gp41-reactive Ab response would take place in the placing of HIV-1 Env vaccination. A DNA leading, recombinant adenovirus serotype 5 (rAd5) increase vaccine that included HIV and genes, and a trivalent combination of clade A, B and C gp140 genes formulated with both gp120 and gp41 elements was examined in the HIV Vaccine Studies Network (HVTN) [stage Ib (HVTN 082), GW-786034 stage II (HVTN 204), stage IIb (HVTN 505) efficiency trial] and various other clinical studies [stage I/II (RV172), stage I (V001)] (4C7). This vaccine was the GW-786034 initial vaccine formulated with the ectodomain from the Env gp41 component, ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
In tumor cells, the p53 pathway is often disrupted. Therefore, recovering the function of wild-type p53 and its targets in tumor cells is a key therapeutic objective. In head and neck cancer, p53 mutations are frequent, and the incidence of p53 mutations increases with progression of head and neck cancer (46, 47). Therefore, a recombinant human adenovirus that expresses functional wild-type p53 has been approved by the Chinese government for the treatment of head and neck carcinoma (48-50). Treatments showed that antitumor efficacy was associated with the expression and activity of functional p53, and adverse effects were also significant (51-54). Recently, pharmacologically activated wild-type p53 by small-molecule compound RITA is reported to inhibit glycolytic enzymes and, therefore, induce robust apoptosis in cancer cells (55). In addition, enhancement of p53 protein stability is also a target in restoring wild-type p53 activity in cancer cells. The protein level of wild-type p53 is ...
Mouse anti Adenovirus Hexon antibody, clone 7C11 reacts with human, canine, bovine, monkey and rat adenoviruses. It is very likely that it
13:2; potential epub Adenovirus notions; tenure bhakti 1? On the epub Adenovirus Methods and of this interest, are above under form. 1282 An epub Adenovirus Methods and Protocols: Adenoviruses, Ad study read by Porten - Szubin 1987:187.
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Greetings- Im interested in the net surface charge on mammalian viruses and how viral charge affects viral adsorption. It is my understanding (correct me if Im wrong) that mammalian viruses have a net negative charge at physiologic pH. I find this interesting because of the net negative charge on mammalian cells due to their phospholipid content. I have been working with human adenovirus gene transfer vectors and have found that the pI of adeno capsid proteins are: Hexon = 4.5 and penton = 4.7. pI of fiber protein on adenovirus is reported to be 7.07. From these data, I have concluded that, at pH 7.0, an adeno virus particle has a negative core (Hexon and penton) with positively charged fiber protrusions. Is this correct (my chemistry is a little rusty)? If so, it would explain how negatively charged adenovirus particles might interact with negatively charged cell surfaces (via positive fiber protein). Thank you, in advance for any comments and corrections. In reading this note, if any related ...
1NLN: Crystallographic structure at 1.6-A resolution of the human adenovirus proteinase in a covalent complex with its 11-amino-acid peptide cofactor: insights on a new fold
We identified previously unrecognized variations in the fiber gene and E4 ORF 6/7 peptides among the various genome types of Ad7. In particular, multiple amino acid changes at E4 ORF 6/7 peptides showed genome type-specific differences between Ad7d and a novel genome type Ad7l. However, no genetic divergence was identified in the hexon gene or in E3 peptides among the 12 Korean strains of Ad7. This result indicates that the nucleotide structures of the fiber gene and of E4 ORF 6/7 peptides might have contributed to the genetic heterogeneity during the Ad7 epidemics in Korea, whereas those of the hexon gene and E3 were highly conserved.. So far, the fiber gene and E4 have rarely been addressed with regard to the evolution and molecular epidemiology of Ad7 strains (17). Restriction analysis of genomic DNA has been the most popular method of describing the molecular epidemiology of the adenoviruses (18). However, little is known about sequence variations with regard to the genome type within an ...
The adenovirus E1B gene products are required for productive infection of human cells and for complete transformation of rodent cells in cooperation with the E1A gene products. Two major, unrelated polypeptides of 55,000 (55K) and 19,000 (19K) daltons are encoded by the E1B region. The 55K protein is required for efficient DNA replication, late mRNA transport to the cytoplasm and shut-off of cellular mRNA transport in productively infected cells. This protein is required for virus-mediated, but not DNA-mediated, transformation of rodent cells. It appears that the 55K protein does not directly contribute to cell transformation, but influences the oncogenicity of adenoviruses when they are inoculated into newborn hamsters. In contrast, the 19K protein is required for adenovirus induced cellular transformation and oncogenicity and localizes to membranes of the nuclear envelope, cytoplasm and the cell surface in transformed cells. This protein affects the efficiency of virus growth in some, but not ...
Looking for online definition of adenovirus early region genes in the Medical Dictionary? adenovirus early region genes explanation free. What is adenovirus early region genes? Meaning of adenovirus early region genes medical term. What does adenovirus early region genes mean?
RIGOTTO, C et al. Evaluation of HA negatively charged membranes in the recovery of human adenoviruses and hepatitis A virus in different water matrices. Mem. Inst. Oswaldo Cruz [online]. 2009, vol.104, n.7, pp.970-974. ISSN 0074-0276. http://dx.doi.org/10.1590/S0074-02762009000700005.. Human adenoviruses (HAdV) and hepatitis A virus (HAV) are shed in the faeces and consequently may be present in environmental waters, resulting in an increase in pathogen concentration that can affect water quality and human health. The aim of this study was to evaluate an adsorption-elution method which utilizes negatively charged membrane HA to determine the efficient recovery of HAdV and HAV from different water matrices and to combine this procedure with a qualitative molecular method (nested RT-PCR and nested PCR). The best efficiency recovery was achieved in distilled water and treated wastewater effluent (100%) for both viruses and in recreational lagoon water for HAV (100%). The efficiency recovery was 10% ...
Human adenovirus, of the Adenoviridae family, is a nonenveloped icosahedral particle containing a single linear dsDNA genome. Human adenovirus comprises 6 species (A through F) consisting of serotypes 1 - 51, and associated with a variety of clinical illnesses. Serotypes 1-39, 42-51 are associated with a variety of respiratory disease, generally in children and immunocompromised persons; whereas serotypes 40 and 41 is associated with enteric disease (enteric adenovirus) particularly in children - these enteric adenoviruses will not be detected with the assay described here. Serotypes 12, 18, 31 have a high oncogenic potential and serotypes 4 and 7 are associated with acute respiratory disease (ARD) frequently in military recruits and typically occur in the winter and spring. Smaller outbreaks of serotypes 3, 4, and 7 occur in the summertime and are associated with contaminated swimming pool water.. Adenovirus infections of the eye may lead to pharyngo-conjunctival fever, follicular ...
Human adenoviruses (HAdVs) cause a wide range of diseases worldwide, including respiratory infections. Studies on HAdV molecular epidemiology are limited in Cameroon. The purpose of this study is to document the different types HAdV circulating in Cameroon in children with acute respiratory infections. Nasopharyngeal swabs were collected from 811 children under 15 years from 2011 to 2014. The HAdV detection was assessed by semi-quantitative generic PCR r-gene®. The HAdV-positive samples were typed by amplification and sequencing of partial hexon gene and a real-time PCR. Demographic data were collected and analyzed. The infection and hospitalization risk factors were assessed thought the Chi-square test. A total of 137/220 HAdV-positive samples were amplified successfully. Six species of HAdV (Mastadenovirus A to F) were detected with B (108/220) and C (47/220) being the predominant strains. Hospitalization and age were significantly associated to HAdV-B and HAdV-C respectively. Phylogenetic analysis
Adenovirus type 37 (Ad37) is a leading cause of epidemic keratoconjunctivitis (EKC), a severe and highly contagious ocular disease. Whereas most other adenoviruses infect cells by engaging CD46 or the coxsackie and adenovirus receptor (CAR), Ad37 binds previously unknown sialic acid-containing cell surface molecules. By glycan array screening, we show here that the receptor-recognizing knob domain of the Ad37 fiber protein specifically binds a branched hexasaccharide that is present in the GD1a ganglioside and that features two terminal sialic acids. Soluble GD1a glycan and GD1a-binding antibodies efficiently prevented Ad37 virions from binding and infecting corneal cells. Unexpectedly, the receptor is constituted by one or more glycoproteins containing the GD1a glycan motif rather than the ganglioside itself, as shown by binding, infection and flow cytometry experiments. Molecular modeling, nuclear magnetic resonance and X-ray crystallography reveal that the two terminal sialic acids dock into two of
RNA molecules from nuclear and cytoplasmic polyribosomes of adenovirus-infected HeLa cells were compared by hybridization to analyse the sequence content. Nuclear polyribosomes were released by exposure of intact detergent-washed nuclei to poly(U) and purified. Cytoplasmic polyribosomes were also purified from the same cells. To show that nuclear polyribosomes contain ribosomes linked by mRNA, polyribosomes were labelled with methionine and uridine in the presence of actinomycin D in adenovirus-infected cells. Purified nuclear polyribosomes were treated with EDTA under conditions which dissociate polyribosomes into ribosomes and subunits with a simultaneous release of mRNA, and sedimented. The treatment dissociated these polyribosomes, releasing the mRNA from them. Radiolabelled total RNA from each polyribosome population was fractionated in sucrose gradients into several pools or hybridized to intact adenovirus DNA to select virus-specific RNA. Sucrose-gradient-fractionated pool-3 RNA (about ...
The utility of recombinant adenovirus serotype 5 (rAd5) vector-based vaccines for HIV-1 and other pathogens will likely be limited by the high prevalence of pre-existing Ad5-specific neutralizing Abs (NAbs) in human populations. However, the immunodominant targets of Ad5-specific NAbs in humans remain poorly characterized. In this study, we assess the titers and primary determinants of Ad5-specific NAbs in individuals from both the United States and the developing world. Importantly, median Ad5-specific NAb titers were ,10-fold higher in sub-Saharan Africa compared with the United States. Moreover, hexon-specific NAb titers were 4- to 10-fold higher than fiber-specific NAb titers in these cohorts by virus neutralization assays using capsid chimeric viruses. We next performed adoptive transfer studies in mice to evaluate the functional capacity of hexon- and fiber-specific NAbs to suppress the immunogenicity of a prototype rAd5-Env vaccine. Hexon-specific NAbs were remarkably efficient at ...
The high prevalence of pre-existing anti-Ad5 immunity in human populations may substantially limit the immunogenicity and clinical utility of rAd5 vector-based vaccines for HIV-1 and other pathogens. Our studies demonstrate ,90% Ad5 seroprevalence in sub-Saharan Africa with median NAb titers ,10-fold higher than those found in the United States. These data suggest that rAd5 vectors should be engineered to evade dominant Ad5-specific NAbs before their use as vaccine vectors in the developing world. To determine the principal targets of Ad5-specific NAbs, we exploited the lack of detectable serologic cross-reactivity between Ad5 and Ad35 (8). Virus neutralization studies using capsid chimeric rAd5/rAd35 vectors and serum samples from both humans and mice demonstrated that Ad5-specific NAbs were directed primarily against the Ad5 hexon protein. Fiber-specific NAbs were detected at low frequencies in vitro but were substantially less efficient than hexon-specific NAbs at blunting rAd5 vaccine ...
Last year, Chiu and colleagues also identified another new adenovirus, named simian adenovirus C, which sickened four of nine captive baboons and killed two of them at a primate facility in 1997. Several staff members at the facility also complained of upper respiratory symptoms at the time of the outbreak. Re-examining the samples many years later, Chiu and his colleagues found antibodies targeted to simian adenovirus C in the human samples.. Chiu concluded that staff members had been exposed to the new virus, and that the virus may have jumped from baboon to human, an idea also supported by follow-up experiments in which laboratory strains of simian adenovirus C efficiently infected both human and baboon cells.. Adenoviruses to date have not generally been linked to cross-species infections between monkeys and humans, Chiu said.. In light of these findings, however, he said the normal vigilance in tracking animal viruses that might also infect humans should extend beyond influenza and ...
Seventy-eight cases were identified in patients from four eye care practices, two family practices, and the hospital emergency department. The median patient age was 45 years (range = 9 months-90 years), and 33 (42%) were men. Ocular signs and symptoms included redness (68%), watery discharge (50%), and pain (29%). Severe signs included corneal infiltrates (17%) and pseudomembranes (6%). At least 12 cases (15%) were health care-associated (Figure). One health care-associated case occurred in a health care worker. Seventeen patients whose infections were not health care-associated reported a symptomatic household or community contact. Among 45 conjunctival swabs available for testing, 19 (42%) were positive for HAdV-8. Genome sequences obtained from five HAdV-8 isolates were 100% identical with one another and showed 97.7% (accession number AB861610.1) to 99.9% (accession number KT340070.1) nucleotide sequence similarity to other HAdV-8 genome sequences available in GenBank, the National ...
Mature human adenovirus particles contain four minor capsid proteins, in addition to the three major capsid proteins (penton base, hexon and fiber) and several proteins associated with the genomic core of the virion. Of the minor capsid proteins, VI plays several crucial roles in the infection cycle of the virus, ... read more including hexon nuclear targeting during assembly, activation of the adenovirus proteinase (AVP) during maturation and endosome escape following cell entry. VI is translated as a precursor (pVI) that is cleaved at both N- and C-termini by AVP. Whereas the role of the C-terminal fragment of pVI, pVIc, is well established as an important co-factor of AVP, the role of the N-terminal fragment, pVIn, is currently elusive. In fact, the fate of pVIn following proteolytic cleavage is completely unknown. Here, we use a combination of proteomics-based peptide identification, native mass spectrometry and hydrogen-deuterium exchange mass spectrometry to show that pVIn is associated ...
Subgroup C adenoviruses, including serotypes 2 and 5, from which most therapeutic adenoviruses are derived, rely on CAR as the primary binding site on the host cell. This receptor has been shown to be crucial for sufficient virus uptake (22) . In cancer cells, however, CAR expression is frequently lost, especially in highly malignant cancer cell lines, leading to a significant decrease in adenovirus uptake (5 , 6) . Our own observations are in agreement with these reports: we frequently found reduced CAR expression at the cell surface in high-grade primary liver cancer and metastases of colorectal cancer. 4 This study investigates the molecular mechanisms involved in reducing CAR expression in cancer cells and explores the possibility of pharmacologically manipulating CAR expression levels.. Increasing evidence exists for a potential physiological role of CAR as a cell adhesion molecule. CAR forms homodimers, was found to physically interact with the tight-junction protein ZO-1, and participates ...
Definition of Adenovirus E3 10.4K/14.5kD Protein in the Legal Dictionary - by Free online English dictionary and encyclopedia. What is Adenovirus E3 10.4K/14.5kD Protein? Meaning of Adenovirus E3 10.4K/14.5kD Protein as a legal term. What does Adenovirus E3 10.4K/14.5kD Protein mean in law?
To delineate the function of adenovirus early region 4 (E4) gene products, we constructed a set of mutant viruses which carry defined lesions within this coding region. Deletion and insertion mutations within six of seven known E4 coding regions had no measurable effect on virus growth in cultured cells. A variant carrying a deletion within the last coding region (encoding a 34,000-molecular-weight polypeptide) was modestly defective, and a mutant lacking the majority of the E4 region was severely defective for growth. The phenotypes of the two defective mutants are similar and complex. Both display perturbations in DNA replication, translation of the E2A mRNA, accumulation of late viral mRNAs, and host cell shutoff. ...
86 PCR positive samples were inoculated onto Hep-2 cell for virus isolation, and 23 HAdV strains were isolated through 7-21 days post-inoculation, with isolation rate of 26.74%, which was close to that of 21.43% in the previous study (Thounaojam et al. 2016). The hexon gene sequence has widely been used for the classification of adenovirus types. In this study, the hexon gene of the 23 HAdV isolates was amplified and sequenced using primer designed by Sarantis et al. (Sarantis et al. 2004) (Forward primer: 5′-CTGATGTACTACAACAGCACTGGCAACATGGG-3′, Reverse primer: 5′-GCGTTGCGGTGGTGGTTAAATGGGTTTACGTTGTCCAT-3′, the amplicon length is 580-605 bp), and the nucleotide sequences were submitted to GenBank (accession numbers MN389388-MN389410). Molecular typing assignments were based on the identity of the closest matching sequences after both BLAST and phylogenetic analysis. Results showed that 9 of the 23 HAdV isolates (8 HAdV-3 and 1 HAdV-7) belonged to species B and 12 of the 23 (5 HAdV-1 and 7 ...
The information presented here serves as a brief introduction to adenovirus, recombinant adenovirus, and their advantages over alternative viral vectors.
The 293 cell line was derived from primary cultures of human embryonic kidney (HEK) cells with sheared fragments of adenovirus (Ad) 5 DNA (Graham et al. 1977). HEK 293 cells contain the nucleotides 1-4344 of Ad5 which are located within the pregnancy-specific ß-1-glycoprotein 4 (PSG 4) gene. The transforming region of the human adenovirus contains the early region (E1), comprising two transcription units, E1a and E1b, whose products are essential and sufficient for mammalian cell transformation by adenoviruses (Louis et al. 1997). Because 293 cells express E1 gene products they are extensively used for the production of E1-deleted Ad viruses. Adeno-associated viruses (AAVs) belong to the family of Parvoviridae, being one of the smallest single-stranded and non-enveloped DNA viruses. AAVss are replication-deficient and have required co-infection with a helper adeno- or herpes virus for productive infection. The AAV Helper-free system takes advantage of the identification of the specific ...
Purification of recombinant adenovirus type 3 dodecahedric virus-like particles for biomedical applications using short monolithic columns
TY - JOUR. T1 - Biobased monoliths for adenovirus purification. AU - Fernandes, Cláudia S. M.. AU - Gonçalves, Bianca. AU - Sousa, Marcos F. Q.. AU - Martins, Duarte L.. AU - Barroso, Telma. AU - Pina, Ana Sofia. AU - Peixoto, Cristina. AU - Aguiar-Ricardo, Ana. AU - Roque, A. Cecília A.. N1 - info:eu-repo/grantAgreement/FCT/COMPETE/132972/PT# info:eu-repo/grantAgreement/FCT/3599-PPCDT/118317/PT# info:eu-repo/grantAgreement/FCT/3599-PPCDT/135802/PT# Sem PDF conforme despacho.. PY - 2015/4/1. Y1 - 2015/4/1. N2 - Adenoviruses are important platforms for vaccine development and vectors for gene therapy, increasing the demand for high titers of purified viral preparations. Monoliths are macroporous supports regarded as ideal for the purification of macromolecular complexes, including viral particles. Although common monoliths are based on synthetic polymers as methacrylates, we explored the potential of biopolymers processed by clean technologies to produce monoliths for adenovirus purification. ...
Adenoviruses were identified as unique viruses in 1953 by Rowe and coworkers when virus was isolated from human adenoids. Because adenoviruses infect various cells they are considered an excellent vector delivery system. A major obstacle to efficient infection by recombinant adenoviruses is the humo.... Full description. ...
Human adenoviruses (HAdVs) often cause mild respiratory infections. These infections, however, can potentially become fatal in immunosuppressive patients. Unfortunately, there has been no specific anti-HAdV drug approved for treatment of HAdV infections. In this study, a time-course transcriptome of HAdV-infected human lung epithelial cells (A549 cells) was performed and compared with perturbation datasets of 890 drug-treated A549 cells from the library of integrated network-based cellular signatures (LINCS) database to predict previously unknown therapeutic drug-HAdV relationships using a characteristic direction (CD) algorithm. We performed experiments to validate a prediction for the anti-diabetic drug rosiglitazone as a candidate drug for treatment of anti-HAdV both in vivo and in vitro. The Type I interferon (IFNs) signaling pathway was negatively regulated during the course of HAdV infection and rosiglitazone increased STAT1 phosphorylation for antiviral IFN response induction. Taken ...
Yakimovich, A; Gumpert, H; Burckhardt, C J; Lutschg, V A; Jurgeit, A; Sbalzarini, I F; Greber, U F (2012). Cell-free transmission of human adenovirus by passive mass transfer in cell culture simulated in a computer model. Journal of Virology, 86(18):10123-10137.. Cardinale, J; Paul, G; Sbalzarini, I F (2012). Discrete region competition for unknown numbers of connected regions. IEEE transactions on image processing : a publication of the IEEE Signal Processing Society, 21(8):3531-3545.. Helmuth, J A; Burckhardt, C J; Greber, U F; Sbalzarini, I F (2009). Shape reconstruction of subcellular structures from live cell fluorescence microscopy images. Journal of Structural Biology, 167(1):1-10.. Helmuth, J A; Burckhardt, C J; Koumoutsakos, P; Greber, U F; Sbalzarini, I F (2007). A novel supervised trajectory segmentation algorithm identifies distinct types of human adenovirus motion in host cells. Journal of Structural Biology, 159(3):347-358.. ...
Abstract Background Viruses bind to specific cellular receptors in order to infect their hosts. The specific receptors a virus uses are important factors in determining host range, cellular tropism, and pathogenesis. For adenovirus, the existing model of entry requires two receptor interactions. First, the viral fiber protein binds Coxsackie and Adenovirus Receptor (CAR), its primary cellular receptor, which docks the virus to the cell surface. Next, viral penton base engages cellular integrins, coreceptors thought to be required exclusively for internalization and not contributing to binding. However, a number of studies reporting data which conflicts with this simple model have been published. These observations have led us to question the proposed two-step model for adenovirus infection. Results In this study we report that cells which express little to no CAR can be efficiently transduced by adenovirus. Using competition experiments between whole virus and soluble viral fiber protein or ...
ViroTag® ADVX (for manual sampling) utilizes a fluorescently-labeled, high-affinity antibody which binds to a unique epitope specifically expressed on adenovirus. The product has been shown to quantify multiple serotypes (2-6) and is considered pan-reactive. With the Virus Counter 3100, use this rapid, no-wash labeling procedure and take adenovirus quantification to new levels of accuracy, speed and simplicity!. Product specifications: The ViroTag ADVX kit (catalog number 92097) contains all reagents and consumables necessary to analyze 200 samples using the Virus Counter 3100 instrument for manual sampling, including:. ...
This 2008 Molecular Cancer Therapeutics paper by Yacoub, A., etc. utilizes the following products and services from Vector Biolabs: Akt1 (dn) Adenovirus, CrmA Adenovirus, Caspase 9 (dn) Adenovirus, human BCL-xL shRNA Adenovirus, Baculoviral IAP repeat-containing 4 Adenovirus, CASP8 and FADD-like apoptosis regulator Adenovirus, MEK1 (dn) Adenovirus.
Adenovirus and pregnancy - What is adenovirus infection? Many forms. This virus can produce several forms of infection. Pharyngo-conjunctival fever (or swimming pool fever) is one of the better known, but also gastroenteritis, upper respiratory tract infections and pneumonia can all occur.
Adenovirus Early Proteins: Proteins encoded by adenoviruses that are synthesized prior to, and in the absence of, viral DNA replication. The proteins are involved in both positive and negative regulation of expression in viral and cellular genes, and also affect the stability of viral mRNA. Some are also involved in oncogenic transformation.
Trentin, J J. and Bryan, E, Immunization of hamsters and histoisogenic mice against trans- plantation of tumors induced by human adenovirus type 12. Abstr. (1964). Subject Strain Bibliography 1964. 1087 ...
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Berget, S.M., Sharp, P.A. (1977) A spliced sequence at the 5′-terminus of adenovirus late mRNA. Brookhaven Symp Biol, 29:332-44.. Berk, A.J., Sharp, P.A. (1977) Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell 12(3): 721-32.. Chow, L.T., Roberts, J.M., Lewis, J.B., Broker, T.R. (1977) A map of cytoplasmic RNA transcripts from lytic adenovirus type 2, determined by electron microscopy of RNA:DNA hybrids. Cell, 11(4): 819-36.. Gibbs, W.W. (2003) The unseen genome: gems among the junk. Scientific American 289(5):26-33.. Hooks, K. B., Delneri, D. & Grifths-Jones, S. (2014) Intron evolution in Saccharomycetaceae. Genome Biol. Evol. 6, 2543-2556.. Kabat, J.L., Barberan-Soler, S., McKenna, P., Clawson, H., Farrer, T., and Zahler, A.M. (2006) Intronic Alternative Splicing Regulators Identified by Comparative Genomics in Nematodes. PLoS Computational Biology 2(7):734-747.. Kiss, T. and Filipowicz, W. (1995) Genes and Development ...
Adenovirus titration kits accurately measure functional titer of your adenovirus preparation using a hexon antibody, or by SYBR Green-based, real-time qPCR in which the virus samples Ct is compared to a standard curve.
Adenovirus titration kits accurately measure functional titer of your adenovirus preparation using a hexon antibody, or by SYBR Green-based, real-time qPCR in which the virus samples Ct is compared to a standard curve.
... belongs to the Adenoviridae family, and along with human adenovirus 40, is a member of species human ... Human adenovirus 41 (HAdV-F41), is an enteric Adenovirus, a nonenveloped virus with an icosahedral nucleocapsid containing a ... "Candidate adenoviruses 40 and 41: fastidious adenoviruses from human infant stool". Journal of Medical Virology. 11 (3): 215- ... As of August 2022, human adenovirus 41 has been identified in a majority of small children with hepatitis of unknown cause in 2 ...
"Genomic organization and chromosomal localization of the human Coxsackievirus B-adenovirus receptor gene". Human Genetics. 105 ... Coxsackievirus and adenovirus receptor (CAR) is a protein that in humans is encoded by the CXADR gene. The protein encoded by ... Tomko RP, Xu R, Philipson L (Apr 1997). "HCAR and MCAR: the human and mouse cellular receptors for subgroup C adenoviruses and ... Tomko RP, Xu R, Philipson L (Apr 1997). "HCAR and MCAR: the human and mouse cellular receptors for subgroup C adenoviruses and ...
The majority of work on adenovirus transcription has focused on human adenovirus, specifically Ad2 and Ad5. The complete ... Ad5 refers to a specific group of human adenoviruses. In Ad5-infected cells, E1A translation was hypothesized to involve the ... Hendrickx R, Stichling N, Koelen J, Kuryk L, Lipiec A, Greber UF (April 2014). "Innate immunity to adenovirus". Human Gene ... Adenovirus early region 1A (E1A) is a gene expressed during adenovirus replication to produce a variety of E1A proteins. It is ...
"Human Adenovirus E Genome". NCBI. Retrieved 2013-01-17. "Human adenovirus E overview". NCBI. Retrieved 2013-01-17. " ... "Protein Details for Human adenovirus E". NCBI. Retrieved 2013-01-17. Russell, WC (Jan 2009). "Adenoviruses: update on structure ... The example used for the following description is Human adenovirus E, a mastadenovirus with a 36 Kbp genome containing 38 ... The 38 genes in the Human adenovirus E genome are organized in 17 transcription units, each containing 1-8 coding sequences. ...
... in humans are generally caused by Adenoviruses types B, C, E and F. Spread occurs mainly when an infected ... Adenovirus infection in humans are generally caused by Adenoviruses types B, C, E and F. Although epidemiologic characteristics ... "Adenovirus: preventing and treating Adenovirus". www.cdc.gov. 29 November 2021. Retrieved 7 May 2022. "Adenovirus". www.cdc.gov ... Adenovirus eye infection may present as a pinky-red eye. Six to nine-days following exposure to adenovirus, one or both eyes, ...
"An Adenovirus Mutant That Replicates Selectively in p53- Deficient Human Tumor Cells". Science. 274 (5286): 373-376. Bibcode: ... Adenoviruses have so far been through three generations of development. Some of the strategies for modification of adenoviruses ... and the knob domain of the adenovirus coat protein trimer. CAR is necessary for adenovirus infection. Although expressed widely ... Adenovirus varieties have been explored extensively as a viral vector for gene therapy and also as an oncolytic virus. Of the ...
Although these infections are common in humans, they are rarely fatal. Symptoms include: Cold symptoms, such as coughing, sore ... Adenovirus serotype 14 (Ad14) is a serovar of adenovirus which, unlike other adenovirus serovars, is known to cause potentially ... "Killer Cold Virus: Adenovirus Infection (Ad14)". Medicine Net. Retrieved May 10, 2015. "Boot camp flu spreads at Lackland". Air ... Of patients tested, 268 were positive for adenovirus. Of 118 serotyped patients, 106 (90%) were identified as Ad14. A total of ...
The most common serogroups are serogroup 1, 2 and 3. No evidence of transmission from birds to humans has been identified. The ... Adenoviruses, accessed 18/08/2011. Adenovirus Taxonomy 2005 Diagnosis of Adenovirus Infections in Psittacine Birds by DNA in ... Aviadenoviruses are adenoviruses that affect birds-particularly chickens, ducks, geese, turkeys and pheasants. There are 15 ... Avian adenoviruses have a worldwide distribution and it is common to find multiple species on a single farm. ...
Human adenovirus 36 (HAdV-36) or Ad-36 or Adv36 is one of 52 types of adenoviruses known to infect humans. AD-36, first ... To date, AD-36 is the only human adenovirus that has been linked with human obesity, present in 30% of obese humans and 11% of ... 2007). "Human adenovirus Ad-36 induces adipogenesis via its E4 orf-1 gene". Int J Obes (Lond). 32 (3): 397-406. doi:10.1038/sj. ... Augustus A.S., Atkinson R.L.; Dhurandhar N.V.; Allison D.B.; Bowen R.L.; Israel B.A.; Albu J.B. (2005). "Human adenovirus-36 is ...
Siadenovirus Testadenovirus In humans, currently there are 88 human adenoviruses (HAdVs) in seven species (Human adenovirus A ... Adenoviruses Stanford University-Adenoviruses Adenoviruses General Concepts General information on Adenovirus DNA virus ... The two currently established receptors are: CD46 for the group B human adenovirus serotypes and the coxsackievirus/adenovirus ... Tupaia adenovirus (TAV) (tree shrew adenovirus 1) has been isolated from tree shrews. Otarine adenovirus 1 has been isolated ...
"Candidate adenoviruses 40 and 41: Fastidious adenoviruses from human infant stool". Journal of Medical Virology. 11 (3): 215- ... "Candidate adenoviruses 40 and 41: fastidious adenoviruses from human infant stool". Journal of Medical Virology. 11 (3): 215- ... She was part of the group that first identified Human adenovirus 41 in children with diarrhoea in 1983. Kapsenberg is ... ISBN 90-5699-556-1. Flint, S. Jane; Nemerow, Glen R. (2017). "8. Pathogenesis". Human Adenoviruses: From Villains To Vectors. ...
The name Mastadenovirus is derived from the Greek word mastos 'breast' (hence mammal) and adenovirus, named for the human ... A Human mastadenovirus A Human mastadenovirus B Human mastadenovirus C Human mastadenovirus D Human mastadenovirus E Human ... can cause potentially fatal adenovirus infections.[citation needed] Canine adenovirus 1 (CAdV-1) can lead to death in puppies, ... Humans and other mammals serve as natural hosts. There are 51 species in this genus. The genus as a whole includes many very ...
He was a pioneer in research on adenoviruses and their role in human diseases. The discoveries of adenoviruses by Rowe et al. ( ... "Serologic Evidence for Human Infection with Adenovirus-Associated Viruses". JNCI: Journal of the National Cancer Institute. ... Huebner, R. J.; Rowe, W. P.; Lane, W. T. (1962). "Oncogenic Effects in Hamsters of Human Adenovirus Types 12 and 18". ... Hoggan, M. D.; Blacklow, N. R.; Rowe, W. P. (1966). "Studies of small DNA viruses found in various adenovirus preparations: ...
They also emerged as a vehicle for human gene therapy Also in the early 1960s, Green and others showed that human adenoviruses ... In 1962, scientists at the National Institutes of Health discovered that certain serotypes of human adenoviruses can induce ... His major focus of investigation was human adenoviruses. He and his colleagues performed foundational viral research; growing ... Doerfler, W. (2012-12-06). The Molecular Biology of Adenoviruses I: 30 Years of Adenovirus Research 1953-1983. Springer Science ...
Ras-related GTP binding C, also known as RRAGC, is a protein which in humans is encoded by the RRAGC gene. RRAGC is a monomeric ... Horwitz MS (Jan 2001). "Adenovirus immunoregulatory genes and their cellular targets". Virology. 279 (1): 1-8. doi:10.1006/viro ... Sekiguchi T, Todaka Y, Wang Y, Hirose E, Nakashima N, Nishimoto T (Feb 2004). "A novel human nucleolar protein, Nop132, binds ... v t e (Articles with short description, Short description matches Wikidata, Genes on human chromosome 1, All stub articles, ...
... implication for adenovirus-mediated gene therapy". Human Gene Therapy. 10 (6): 957-64. doi:10.1089/10430349950018355. PMID ... "Expression of the peptide antibiotics human beta defensin-1 and human beta defensin-2 in normal human skin". The Journal of ... Hiratsuka T, Nakazato M, Ashitani J, Matsukura S (February 1999). "[A study of human beta-defensin-1 and human beta-defensin-2 ... Liu L, Zhao C, Heng HH, Ganz T (August 1997). "The human beta-defensin-1 and alpha-defensins are encoded by adjacent genes: two ...
2005). "A human protein-protein interaction network: a resource for annotating the proteome". Cell. 122 (6): 957-68. doi: ... It may play an important role in nucleocytoplasmic RNA transport, and its function is modulated by E1B-55kDa in adenovirus- ... Heterogeneous nuclear ribonucleoprotein U-like protein 1 is a protein that in humans is encoded by the HNRNPUL1 gene. This gene ... 2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci ...
The human PSMC1 gene has 11 exons and locates at chromosome band 14q32.11. The human protein 26S protease regulatory subunit 4 ... "Regulation of the 26S proteasome by adenovirus E1A". EMBO J. 19 (17): 4759-73. doi:10.1093/emboj/19.17.4759. PMC 302057. PMID ... Madani N, Kabat D (1998). "An endogenous inhibitor of human immunodeficiency virus in human lymphocytes is overcome by the ... In humans the 26S protease regulatory subunit 4', also known as 26S proteasome AAA-ATPase subunit Rpt2, is an enzyme that is ...
... and adenovirus 11. AHC can only exist in a human host and is transmitted through human contact with an infected individual or ... Adenoviruses are a medium-sized variant that are nonenveloped, like Enterovirus 70. They have a double-stranded linear DNA ... Its isolation host is human. It is an antigenic variant of the Coxsackievirus A24. It was studied independently for the first ... There are 57 variants of this virus, that each cause 5-10% of upper respiratory infections in humans. These viruses are some of ...
Kidd, AH; Garwicz D; Oberg M (1995). "Human and simian adenoviruses: phylogenetic inferences from analysis of VA RNA genes". ... These two VA RNA genes are distinct genes in the adenovirus genome. VA RNAI is the major species with VA RNAII expressed at a ... The VA (viral associated) RNA is a type of non-coding RNA found in adenovirus. It plays a role in regulating translation. There ... Ma, Y; Mathews MB (1996). "Secondary and tertiary structure in the central domain of adenovirus type 2 VA RNA I". RNA. 2 (9): ...
"A family of human cdc2-related protein kinases." The EMBO Journal 11.8 (1992): 2909. Whyte, Peter, Nicola M. Williamson, and E ... Whyte, P (1988). "Association between an oncogene and an anti-oncogene: the adenovirus E1A proteins bind to the retinoblastoma ... "The human papilloma virus-16 E7 oncoprotein is able to bind to the retinoblastoma gene product." Science 243.4893 (1989): 934- ... Dyson, N (1989). "The human papilloma virus-16 E7 oncoprotein is able to bind to the retinoblastoma gene product". Science. 243 ...
A common helper virus in humans is the adenovirus.[citation needed] Dependoparvovirus is not infectious enough to trigger an ... Viral vectors are currently being developed to transport genes into human cells. Since this virus does not stimulate an immune ... they cannot replicate productively in their host cell without the cell being coinfected by a helper virus such as an adenovirus ...
... including specific infectious diseases and classes thereof List of human disease case fatality rates - List of human disease ... oral adenovirus type 4 and type 7 vaccine, in U.S. Military recruits". Vaccine. 31 (28): 2963-2971. doi:10.1016/j.vaccine. ... "Human hookworm vaccine trial". Medical News Today. January 20, 2012. Retrieved April 24, 2013. Hanabuchi, S.; Ohashi, T.; Koya ... De Thé, G.; Bomford, R.; Kazanji, M.; Ibrahim, F. (2007). "Human T Cell Lymphotropic Virus: Necessity for and Feasibility of a ...
Diseases such as HIV and human adenoviruses have been associated with NHP interactions. In places where contact between humans ... Most viral diseases of humans are zoonotic in origin, having been historically transmitted to human populations from various ... and humans, disease transmission between NHP and humans is relatively common and can become a major public health concern. ... Humans usually become infected through the bite of an infected rodent flea." The sanitary control measure instituted by the ...
Within six hours, 99.999% of the adenovirus particles were inactivated. Within six hours, 50% of the infectious adenovirus ... Michels, H. T.; Wilks, S. A.; Noyce, J. O.; Keevil, C. W. (2005), Copper Alloys for Human Infectious Disease Control Archived ... Adenovirus is a group of viruses that infect the tissue lining membranes of the respiratory and urinary tracts, eyes, and ... Adenoviruses account for about 10% of acute respiratory infections in children.[citation needed] These viruses are a frequent ...
Pirozzi G, McConnell SJ, Uveges AJ, Carter JM, Sparks AB, Kay BK, Fowlkes DM (Jun 1997). "Identification of novel human WW ... Galinier R, Gout E, Lortat-Jacob H, Wood J, Chroboczek J (2003). "Adenovirus protein involved in virus internalization recruits ... Portal: Biology v t e (Articles with short description, Short description matches Wikidata, Genes on human chromosome 16, EC ... Jonsson AB (1998). "Identification of a human cDNA clone that mediates adherence of pathogenic Neisseria to non-binding cells ...
"Immune responses to adenovirus and adeno-associated virus in humans". Gene Therapy. 6 (9): 1574-83. doi:10.1038/sj.gt.3300994. ... The adeno-associated virus serotype 1 (AAV1) viral vector delivers an intact copy of the human lipoprotein lipase (LPL) gene to ... their findings were featured on the September 2004 cover of Human Gene Therapy. Ross and Hayden next succeeded in treating cats ...
This feature, along with the ability to infect quiescent cells present their dominance over adenoviruses as vectors for human ... Samulski RJ (2003). "AAV vectors, the future workhorse of human gene therapy". Human Gene Therapy: Current Opportunities and ... "Immune responses to adenovirus and adeno-associated virus in humans". Gene Therapy. 6 (9): 1574-83. doi:10.1038/sj.gt.3300994. ... Recent human clinical trials using AAV for gene therapy in the retina have shown promise. The adeno-associated virus (AAV), ...
Gam-COVID-Vac is a viral vector vaccine based on two recombinant replication-defective human adenoviruses: Ad26 (serotype 26) ... On 4 March 2021, the Committee for Medicinal Products for Human Use (CHMP) of the EMA started a rolling review of Sputnik V. ... Adenovirus infections cause only mild colds in healthy individuals, but they can cause life-threatening illnesses in ... One is called Janssen COVID-19 vaccine, it utilizes the Ad26COV2 viral vector based on the human virus Ad26. For this vaccine, ...
Shaw G, Morse S, Ararat M, Graham FL (June 2002). "Preferential transformation of human neuronal cells by human adenoviruses ... "Characteristics of a human cell line transformed by DNA from human adenovirus type 5". The Journal of General Virology. 36 (1 ... Adenoviruses transform neuronal lineage cells much more efficiently than typical human kidney epithelial cells. An embryonic ... Louis N, Evelegh C, Graham FL (July 1997). "Cloning and sequencing of the cellular-viral junctions from the human adenovirus ...
Outside the human body, the virus is destroyed by household soap, which bursts its protective bubble. SARS-CoV-2 is closely ... Due to overlap with other infections such as adenovirus, imaging without confirmation by rRT-PCR is of limited specificity in ... Human angiotensin converting enzyme 2 (hACE2) is the host factor that SARS-CoV-2 virus targets causing COVID‑19. Theoretically ... After strict quarantines and a mink euthanasia campaign, the cluster was assessed to no longer be circulating among humans in ...
In contrast to medical wisdom in the 1960s and 1970s, Huebner was confident that viruses were a cause of cancer in humans and ... From that culture they isolated cytomegalovirus, as well as the first of a large family of adenoviruses. Dr. Robert M. Chanock ...
... often leading to disease phenotypes in humans and other animals. Insertion of Alu elements in the human genome is associated ... and relief of repression by adenovirus E1A protein". Cell. 67 (2): 377-88. doi:10.1016/0092-8674(91)90189-6. PMID 1655281. ... There are >50 human diseases associated with SINEs. When inserted near or within the exon, SINEs can cause improper splicing, ... Shi Y, Seto E, Chang LS, Shenk T (October 1991). "Transcriptional repression by YY1, a human GLI-Krüppel-related protein, ...
... +protein,+human at the US National Library of Medicine Medical Subject Headings (MeSH) This article incorporates text from ... They function as cellular transcription factors and as replication factors for adenovirus DNA replication. Diversity in this ... Nuclear factor 1 A-type is a protein that in humans is encoded by the NFIA gene. Nuclear factor I (NFI) proteins constitute a ... Champ PC, Maurice S, Vargason JM, Camp T, Ho PS (2005). "Distributions of Z-DNA and nuclear factor I in human chromosome 22: a ...
Mali S (January 2013). "Delivery systems for gene therapy". Indian Journal of Human Genetics. 19 (1): 3-8. doi:10.4103/0971- ... Viruses used for gene therapy to date include retrovirus, adenovirus, adeno-associated virus and herpes simplex virus. However ... lentiviral and human foamy virus are RNA-based viral vectors that reverse transcript and integrated into the host genome, ... Committee on Identifying and Assessing Unintended Effects of Genetically Engineered Foods on Human Health (2004-01-01). Methods ...
"The Human Protein Atlas". www.proteinatlas.org. Archived from the original on 2017-05-01. Retrieved 2017-04-27. Hirokawa N, ... Some viruses (including retroviruses, herpesviruses, parvoviruses, and adenoviruses) that require access to the nucleus to ... while others such as humans use an analogous complex called HAUS) acts an additional means of microtubule nucleation in the ... "The augmin complex plays a critical role in spindle microtubule generation for mitotic progression and cytokinesis in human ...
Chang CJ, Chao JC (April 2002). "Effect of human milk and epidermal growth factor on growth of human intestinal Caco-2 cells". ... Wang N, Verna L, Hardy S, Forsayeth J, Zhu Y, Stemerman MB (September 1999). "Adenovirus-mediated overexpression of c-Jun and c ... Kim S, Yu SS, Lee IS, Ohno S, Yim J, Kim S, Kang HS (April 1999). "Human cytomegalovirus IE1 protein activates AP-1 through a ... AP-1 was first discovered as a TPA-activated transcription factor that bound to a cis-regulatory element of the human ...
Human Genome Project - hybridization - hydrocarbon - hydrogen - hydrogen bond - hydrogenation - hydrogen-deuterium exchange - ... adenovirus - adrenergic receptor - adrenodoxin - aequorin - aerobic respiration - agonist - alanine - albumin - alcohol - ...
Numerous human pathogenic viruses in circulation are encased in lipid bilayers, and they infect their target cells by causing ... DNA viruses Adenoviruses Parvoviruses Polyomaviruses Anelloviruses RNA viruses Caliciviruses Picornaviruses Reoviruses ... One of the main parts of human pathogenic viruses is glycoprotein. They have been shown to play significant roles in immunity ... To create potentially protective vaccines for human pathogenic enveloped viruses for which there is currently no vaccine, it is ...
... of subgroup C human adenoviruses, in species with a deficient CAAT sequence. The transcription initiation at mutant MLP species ... "Functional Analysis of the CAAT Box in the Major Late Promoter of the Subgroup C Human Adenoviruses". Journal of Virology. 72 ( ... In another experiment performed with the major late promoter (MLP) of adenoviruses from a variety of host species, it was shown ... The failure to restore the normally functional adenoviruses, exhibited by a CAAT box, is consistent with the idea that the CAAT ...
1989). "Two human genes isolated by a novel method encode DNA-binding proteins containing a common region of homology". Gene. ... YB-1 helps the replication of adenovirus type 5, a commonly used vector in gene therapy. Thus, YB-1 can cause an "oncolytic" ... YBX1+protein,+human at the US National Library of Medicine Medical Subject Headings (MeSH) This article incorporates text from ... Horwitz EM, Maloney KA, Ley TJ (1994). "A human protein containing a "cold shock" domain binds specifically to H-DNA upstream ...
"Prevalence of the initiator over the TATA box in human and yeast genes and identification of DNA motifs enriched in human TATA- ... For example, one study used the adenovirus TATA promoter sequence (5'-CGCTATAAAAGGGC-3') as a model binding sequence and found ... Similarly, in humans only 24% of genes have promoter regions containing the TATA box. Genes containing the TATA-box tend to be ... Nikolov DB, Chen H, Halay ED, Hoffman A, Roeder RG, Burley SK (May 1996). "Crystal structure of a human TATA box-binding ...
... adenovirus, severe acute respiratory syndrome (SARS), Middle East respiratory syndrome (MERS), COVID-19 and measles. Chest ... "Discovery of a novel coronavirus associated with the recent pneumonia outbreak in humans and its potential bat origin". bioRxiv ...
Sartori AA, Lukas C, Coates J, Mistrik M, Fu S, Bartek J, Baer R, Lukas J, Jackson SP (November 2007). "Human CtIP promotes DNA ... "Interaction between a cellular protein that binds to the C-terminal region of adenovirus E1A (CtBP) and a novel cellular ... Retinoblastoma-binding protein 8 is a protein that in humans is encoded by the RBBP8 gene. The protein encoded by this gene is ... "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine. "Mouse PubMed ...
... is an adenovirus protein of 14kDa which regulates growth in all stages of the adenovirus (Ad) infection. E4orf4 partners mainly ... In humans, the Noxa homologue is known as APR/PMAIP1. Upon receiving intrinsic death signals, the gene NOXA encodes for the ... Toolan, H.W., Saunders, E.L., Southam, C.M., Moore, A.E. and Levin, A.G. (1965) H-l virus viremia in the human. Proc. Sot. Exp ... Conserved domains among human, mouse, and rat homologs include the leucine zipper (LZ) domain at the C-terminal region, two ...
Wang C, Chao J, Chao L (Apr 2003). "Adenovirus-mediated human prostasin gene delivery is linked to increased aldosterone ... Prostasin is a protein that in humans is encoded by the PRSS8 gene. This gene encodes a trypsinogen, which is a member of the ... Yu JX, Chao L, Ward DC, Chao J (Mar 1996). "Structure and chromosomal localization of the human prostasin (PRSS8) gene". ... Yu JX, Chao L, Chao J (Jul 1994). "Prostasin is a novel human serine proteinase from seminal fluid. Purification, tissue ...
There are few examples of the use of intraperitoneal injections in humans cited in literature because it is mainly used to ... "Phase I Trial of Intraperitoneal Injection of the E1B-55-kd-Gene-Deleted Adenovirus ONYX-015 (dl1520) Given on Days 1 Through 5 ... Figure 1.0 roughly depicts where the injection would be administered to the peritoneal cavity in humans. One study utilized IP ... Overall, this section provides a few examples of the effects and uses of intraperitoneal injections in human patients. There ...
"Serotype chimeric oncolytic adenovirus coding for GM-CSF for treatment of sarcoma in rodents and humans". International Journal ... It is modified to selectively replicate in p16/Rb-defective cells, which include most human cancer cells. In addition, ONCOS- ... While the ONCOS-102 oncolytic adenovirus has shown efficacy as a single agent against several soft tissue sarcomas, it would ... ONCOS-102 (Ad5/3-D24-GMCSF), (developed by Oncos Therapeutics) is an oncolytic adenovirus previously described as CGTG-102. It ...
Other agents that cause this illness include human metapneumovirus, influenza, parainfluenza, coronavirus, adenovirus, ... Acute bronchiolitis is usually the result of infection by respiratory syncytial virus (72% of cases) or human rhinovirus (26% ... This is most commonly caused by respiratory syncytial virus (RSV, also known as human pneumovirus). ...
"Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs". Genome ... Gregor PD, Sawadogo M, Roeder RG (Oct 1990). "The adenovirus major late transcription factor USF is a member of the helix-loop- ... Upstream stimulatory factor 1 is a protein that in humans is encoded by the USF1 gene. This gene encodes a member of the basic ... USF1 (human gene) has been shown to interact with USF2, FOSL1 and GTF2I. GRCh38: Ensembl release 89: ENSG00000158773 - Ensembl ...
Principles of Epidemiology in Public Health Practice (3rd ed.), U.S. Department of Health and Human Services and Centers for ... Implications of the Adenovirus Vector Experience". Science. 344 (6179): 49-51. doi:10.1126/science.1250672. ISSN 0036-8075. PMC ... US Department of Health and Human Services. 2016-01-29. Retrieved 2020-05-06. Ferreira, Juliana Carvalho; Patino, Cecilia Maria ...
Zinc finger MYND domain-containing protein 11 is a protein that in humans is encoded by the ZMYND11 gene. The protein encoded ... Isobe T, Uchida C, Hattori T, Kitagawa K, Oda T, Kitagawa M (Jan 2006). "Ubiquitin-dependent degradation of adenovirus E1A ... v t e (Articles with short description, Short description matches Wikidata, Genes on human chromosome 10, All stub articles, ... Masselink H, Bernards R (Mar 2000). "The adenovirus E1A binding protein BS69 is a corepressor of transcription through ...
The vector is a non-replicating adenovirus 5. The payload is a chimeric gene encoding a fusion protein that combines the ... extracellular and intramembrane domains of the human TNF receptor 1 and the intracellular domain of the Fas receptor, under the ...
PEGylation of adenoviruses for gene therapy can help prevent adverse reactions due to pre-existing adenovirus immunity. A ... Bowman L (4 December 2004). "Study on dogs yields hope in human paralysis treatment". Seattle Post-Intelligencer. Blume G, Cevc ... Seregin SS, Amalfitano A (2009). "Overcoming pre-existing adenovirus immunity by genetic engineering of adenovirus-based ... May 2010). "Postexposure protection of non-human primates against a lethal Ebola virus challenge with RNA interference: a proof ...
A human Anellovirus called TTV is included within this classification and is found in almost all humans, infecting them ... Some of these viruses require host cell polymerases to replicate their genome, while others, such as adenoviruses or herpes ...
"RNA polymerase III transcription from the human U6 and adenovirus type 2 VAI promoters has different requirements for human BRF ... FactorBook BRF1 Human BRF1 genome location and BRF1 gene details page in the UCSC Genome Browser. v t e (Articles with short ... Transcription factor IIIB 90 kDa subunit is a protein that in humans is encoded by the BRF1 gene. This gene encodes one of the ... Cabart P, Murphy S (2002). "Assembly of human small nuclear RNA gene-specific transcription factor IIIB complex de novo on and ...
Directed evolution was applied on human adenovirus, one of many viruses that are being developed as oncolytic agents, to create ... An oncolytic adenovirus, a genetically modified adenovirus named H101, was approved in China in 2005 for the treatment of head ... in human non-small cell lung cancer". Human Gene Therapy. 10 (18): 3013-29. doi:10.1089/10430349950016410. PMID 10609661. S2CID ... This hybrid of adenovirus serotypes Ad11p and Ad3 shows much higher potency and tumour selectivity than the control viruses ( ...
Some of the largest genomes of virophages are similar to the genome size of an adenovirus. All virophages known so far have ... genomes from diverse habitats including the human gut, plant rhizosphere, and terrestrial subsurface, from 27 distinct ...
Genome variability of human adenovirus type 8 causing epidemic keratoconjunctivitis during 1986-2003 in Japan. Xue-Hai Jin,1 ... Human adenovirus type 8 (HAdV-8) is one of the major serotypes isolated from patients with EKC. DNA restriction enzyme analyses ... Ishiko H, Shimada Y, Konno T, Hayashi A, Ohguchi T, Tagawa Y, Aoki K, Ohno S, Yamazaki S. Novel human adenovirus causing ... Human adenoviruses (HAdVs) cause ocular infections. The most severe disease among ocular infections is epidemic ...
IgG-Complexed Adenoviruses Induce Human Plasmacytoid Dendritic Cell Activation and Apoptosis. Thi Thu Phuong Tran 1 2 , Tuan ...
Metagenomic analysis identifies human adenovirus 31 in children with acute flaccid paralysis in Tunisia. ... Metagenomic analysis showed that adenovirus sequences that were closely to the A31 and A61 genotypes were the most abundant. ... A metagenomics approach, first used on five pools of four samples each, revealed the presence of adenovirus sequences. ...
... named Mobilan that drives expression of self-activating TLR5 signaling cassette comprising of human TLR5 and a secreted ... we created an adenovirus-based vector for intratumor delivery, ... animal models and to be safe in both animals and humans. ... of human coxsackievirus and adenovirus receptor is associated with efficient adenovirus-mediated transgene expression in human ... Mouse and human prostate tumors express CAR and are efficiently infected by Ad-mCherry. (a) A representative area of a human ...
Investigation of promoter function in human and animal cells infected with human recombinant adenoviruses expressing rotavirus ...
Our understanding of adenovirus (Ad) biology is largely extrapolated from human species C Ad5. Most humans are immune to Ad5, ... Comparison of the life cycles of genetically distant species C and species D human adenoviruses Ad6 and Ad26 in human cells. ... Comparison of the life cycles of genetically distant species C and species D human adenoviruses Ad6 and Ad26 in human cells. / ... Comparison of the life cycles of genetically distant species C and species D human adenoviruses Ad6 and Ad26 in human cells. In ...
"ADENOVIRUS TYPE 23, Strain AV-2732 Adenovirus,human" ATCC VR-258_FD. Szukaj ... "ADENOVIRUS TYPE 23, Strain AV-2732 Adenovirus,human" ATCC VR-258_FD ... PhD Student position in Dioscuri Centre of Scientific Excellence for RNA-Protein Interactions in Human Health and Disease , ... PhD Student position in Dioscuri Centre of Scientific Excellence for RNA-Protein Interactions in Human Health and Disease The ...
Adenoviruses, Human Adenovirus Infections, Human Child Expedited Gastroenteritis Genes, Viral Genome, Viral Humans Molecular ... Title : Novel Human Adenovirus Strain, Bangladesh Personal Author(s) : Matsushima, Yuki;Shimizu, Hideaki;Kano, Atsuko;Nakajima ... We report a novel human adenovirus D (HAdV-65) isolated from feces of 4 children in Bangladesh who had acute gastroenteritis. ... U.S Department of Health & Human Services USA.gov CDC Website Exit Disclaimer Version 3.21 ...
Replication-deficient recombinant adenoviruses expressing the human immunodeficiency virus Env antigen can induce both humoral ... Replication-deficient recombinant adenoviruses expressing the human immunodeficiency virus Env antigen can induce both humoral ...
... , Virology, April 1988, ... A simple technique for the rescue of early region I mutations into infectious human adenovirus type 5. ...
Reported by: Oregon Dept of Human Svcs. Washington State Dept of Health Communicable Diseases. 37th Training Wing, 59th ... Adenovirus serotype 14 (Ad14) is a rarely reported but emerging serotype of adenovirus that can cause severe and sometimes ... where adenovirus was detected by polymerase chain reaction (PCR). Adenovirus also was isolated by culture, confirmed by ... adenovirus infections often go undetected, because few laboratories routinely test for adenovirus and even fewer do serotyping ...
Surprisingly, the 243-amino-acid form of adenovirus-5 E1A was found subsequently to reverse-transform many human tumour cells. ... the 243-amino-acid form of adenova-5 E1A was found subsequently to reverse-transform many human tumour cells. The adenovirus ... The adenovirus early region 1A (E1A) proteins were described originally as immortalizing oncoproteins that altered ... Human Adenovirus type 5 (HAdV5) represents a well-studied model system to analyze the transformation capabilities of DNA tumor ...
... was evaluated for the rapid diagnosis of ocular adenovirus infections. In 36 culture-proven cases of adenovirus ocular ... Adenovirus Infections, Human / diagnosis* * Evaluation Studies as Topic * Eye Diseases / diagnosis* * Humans ... was evaluated for the rapid diagnosis of ocular adenovirus infections. In 36 culture-proven cases of adenovirus ocular ... Rapid diagnostic test for ocular adenovirus Ophthalmology. 1988 Apr;95(4):431-3. doi: 10.1016/s0161-6420(88)33168-4. ...
View Mouse Monoclonal anti-Adenovirus Antibody (1E11) (NB200-425). Validated Applications: ELISA, ID, IHC, S-ELISA, LFA. ... This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are ... Blogs on Adenovirus. There are no specific blogs for Adenovirus, but you can read our latest blog posts. ... Reviews for Adenovirus Antibody (NB200-425) (0) There are no reviews for Adenovirus Antibody (NB200-425). By submitting a ...
These primary cell cultures were often noted to spontaneously degenerate over time, and adenoviruses are now known to be a ... Adenovirus, a DNA virus, was first isolated in the 1950s in adenoid tissue-derived cell cultures, hence the name. ... An extremely hardy virus, adenovirus is ubiquitous in human and animal populations, survives long periods outside a host, and ... The first is lytic infection, which occurs when an adenovirus enters human epithelial cells and continues through an entire ...
However, a global view of the effects of Ad5 infection on such programs in normal human cells is not available, despite ... These findings establish that the impact of adenovirus infection on host cell programs is far greater than appreciated hitherto ... normal human fibroblasts. We observed that the expression of some 2,000 genes, about 10% of those examined, increased or ... Human adenoviruses, such as serotype 5 (Ad5), encode several proteins that can perturb cellular mechanisms that regulate cell ...
Identification of human and animal adenoviruses and polyomaviruses for determination of sources of fecal contamination in the ... PCR fecal contamination shellfish adenoviruses JCV water contamination JC hepatitis E polyomaviruses sewage adenovirus HEV More ... Read more about Identification of human and animal adenoviruses and polyomaviruses for determination of sources of fecal ... Potential transmission of human polyomaviruses through the gastrointestinal tract after exposure to virions or viral DNA. ...
According to the results in this study, recombinant human endostatin adenovirus in combination with a low dose of cisplatin ... we injected recombinant human endostatin adenovirus intratumorally plus a low dose of cisplatin intraperitoneally routinely. ... Construction of recombinant adenovirus. Construction of recombinant human endostatin adenovirus has been described in the ... Inhibitive effects of recombinant adenovirus-mediated human endostatin on the growth of human hepatocellular carcinoma ...
Adenovirus. * Influenza. * Human metapneumovirus. In rare cases, bronchiolitis may be caused by bacteria. ...
Acute Hepatitis in Children in 2022 - Human Adenovirus 41?. List of authors.*Saul J. Karpen, M.D., Ph.D. ... A Case Series of Children with Acute Hepatitis and Human Adenovirus Infection. L.H. Gutierrez Sanchez and Others ...
TP53I13 Adenovirus (Human). Tp53i13 adenovirus (human) Catalog: 141219A , Size: 250ul , Price: €517.5 Supplier: abm Adinovirus ... Product type: Adenovirus Detection and sensitivity: Before using TP53I13 Adenovirus (Human) please read the package insert. It ... is intended for Human reactivity. Accession Number: BC001593 Performance and applications: Please contact Gentaurs support by ... TP53I13 Adenovirus (Human) linc-TP53I13, lnc-TP53I13 ...
What is the role of adenoviruses in human health?. Posted on 09/11/2022. ... Home » Health News » What is the role of adenoviruses in human health? ... Almost 200 trials are ongoing of adenovirus-based therapeutics and vaccines.. It was in 2020 that the first adenovirus vaccine ... or recombination of human and animal adenoviruses. This could lead to more severe diseases, for which the world is poorly ...
This is the first DNA-based vaccine approved for human use.. Other Adenovirus Vaccines. ImmunityBio is developing an adenovirus ... Johnson & Johnsons vaccine is an adenovirus vector vaccine using an Ad26 vector (a human adenovirus) believed to have less ... to try to avoid pre-existing immunity in the human population. It is made from an adenovirus that was modified to help human ... It differs from other adenovirus vector vaccines like J&J and AstraZeneca because it uses two adenovirus vectors rather than ...
... early detection at the human-animal interface will provide earlier warning. Here, we review recent emerging virus treats for ... A Mini Review of the Zoonotic Threat Potential of Influenza Viruses, Coronaviruses, Adenoviruses, and Enteroviruses. August 5, ... Among the recently emerged respiratory pathogens are influenza viruses, coronaviruses, enteroviruses, and adenoviruses. As the ... Adenoviruses, and Enteroviruses Emily S. Bailey,1,2,* Jane K. Fieldhouse,1,2 Jessica Y. Choi,1,2 and Gregory C. Gray1,2,3,4 ...
What is the role of adenoviruses in human health? With advances in molecular biology informing recent developments in ... human adenoviruses (HAdVs) have been of primary importance in medical research. A recent review looks at the promises of HAdVs ... The ability to sequence the genomes of these models is a powerful tool to study the genetic factors that impact human health. ... A new study describes the association between monkeypox infection and eye complications in humans. ...
Mechanism of host cell restriction in African green monkey kidney cells abortively infected with human adenovirus type 2. ( ... Mechanism of host cell restriction in African green monkey kidney cells abortively infected with human adenovirus type 2.. ... Interaction of a simian papovavirus and adenoviruses. I. Induction of adenovirus tumor antigen during abortive infection of ... Studies on the mechanism of enhancement of adenovirus 7 infection in African green monkey cells by simian virus 40: formation ...
  • daughter of AQ-1, had a febrile respiratory infection with Human Adenovirus Type 55 cough. (cdc.gov)
  • In 36 culture-proven cases of adenovirus ocular infection, direct Adenoclone testing of conjunctival swabs was positive in 24 of 31 patients (77%) tested within 1 week of onset of symptoms, and in one of five patients (20%) who presented after 1 week (P less than 0.02). (nih.gov)
  • These primary cell cultures were often noted to spontaneously degenerate over time, and adenoviruses are now known to be a common cause of asymptomatic respiratory tract infection that produces in vitro cytolysis in these tissues. (medscape.com)
  • Adenovirus is often cultured from the pharynx and stool of asymptomatic children, and most adults have measurable titers of anti-adenovirus antibodies, implying prior infection. (medscape.com)
  • Recovery from adenovirus infection is associated with the development of serotype-specific neutralizing antibodies. (medscape.com)
  • Upon infection with adenovirus, one of three different interactions with the cells may occur. (medscape.com)
  • The first is lytic infection, which occurs when an adenovirus enters human epithelial cells and continues through an entire replication cycle, which results in cytolysis, cytokine production, and induction of host inflammatory response. (medscape.com)
  • However, a global view of the effects of Ad5 infection on such programs in normal human cells is not available, despite widespread efforts to develop adenoviruses for therapeutic applications. (biomedcentral.com)
  • We used two-color hybridization and oligonucleotide microarrays to monitor changes in cellular RNA concentrations as a function of time after Ad5 infection of quiescent, normal human fibroblasts. (biomedcentral.com)
  • These findings establish that the impact of adenovirus infection on host cell programs is far greater than appreciated hitherto. (biomedcentral.com)
  • A new study describes the association between monkeypox infection and eye complications in humans. (news-medical.net)
  • Studies on the mechanism of enhancement of adenovirus 7 infection in African green monkey cells by simian virus 40: formation of adenovirus-specific RNA. (wikidata.org)
  • HAdV infection is also associated with a serious adenovirus syndrome in immunocompromised patients after stem cell transplantation ( 2 ). (cdc.gov)
  • Replication-defective recombinant adenovirus vectors carrying P. falciparum epitopes may be useful as part of a vaccine that raises cellular immunity to the pre-erythrocytic stage of malaria infection. (ox.ac.uk)
  • The study continues the line of research which linked the virus to animal obesity but is the first to suggest infection may drive weight gain in humans. (news-medical.net)
  • Co-infection rates between the viruses ranged from 1% (hMPV and Adenovirus) to 12% (hMPV and Human Bocavirus). (pediatriceducation.org)
  • In normal non-cancer cells, the oncolytic adenovirus cannot replicate during infection, leaving the cell unaffected. (medicaltrend.org)
  • Jalal H, Bibby DF, Tang JW, Bennett J, Kyriakou C, Peggs K. First reported outbreak of diarrhea due to adenovirus infection in a hematology unit for adults. (medscape.com)
  • Entre le 1er janvier et le 31 décembre 2013, nous avons utilisé les données de la surveillance des patients hospitalisés pour une infection respiratoire aiguë sévère (IRAS) dans trois hôpitaux publics égyptiens dans le district de Damanhour afin d'estimer le taux d'incidence de la grippe saisonnière confirmée en laboratoire. (who.int)
  • The effective rate of influenza reassortment is limited during human infection. (cdc.gov)
  • Endemic Infection of Stranded Southern Sea Otters (Enhydra lutris nereis) with Novel Parvovirus, Polyomavirus, and Adenovirus. (cdc.gov)
  • This investigation focuses on collecting information to describe the epidemiology, etiology, clinical presentation, severity, and risk factors related to illness and to identify any relationship between adenovirus infection or other factors and hepatitis. (cdc.gov)
  • It is an update to a HAN Health Advisory that CDC issued on April 21, 2022 , that initially notified clinicians and public health authorities of children identified with hepatitis of unknown origin and adenovirus infection. (cdc.gov)
  • When the host is infected with adenovirus, TNFSF14 can be used as an inflammatory biomarker to indicate whether there was an adenovirus infection in the host and the degree of disease caused by viral infection. (bvsalud.org)
  • A(H1N2) variant viruses and one human case of infection with an influenza A(H3N2) variant virus were reported officially.3 One additional human case of infection with an influenza A(H1N1)v virus was detected. (who.int)
  • Since the last risk assessment on 21 May 2021, one new laboratory-confirmed human case of influenza A(H5N6) virus infection was reported from China to WHO on 30 May 2021. (who.int)
  • 1. What is the likelihood that additional human cases of infection with avian influenza A(H5) viruses will occur? (who.int)
  • Good quality serological investigations may be useful in differentiating infection from contamination in these cases and allow for better assessment of the risk of human infection. (who.int)
  • Most humans are immune to Ad5, so lower-seroprevalence viruses like human Ad6 and Ad26 are being tested as therapeutic vectors. (elsevier.com)
  • To better understand how this might impact their biology, we examined the life cycle of the two viruses in human lung cells in vitro. (elsevier.com)
  • However, it was the demonstration that some human adenoviruses induce tumors in laboratory animals [ 1 ] that greatly increased interest in these viruses and their interactions with host cells. (biomedcentral.com)
  • HAdVs infect only humans, a pattern common to these highly species-specific viruses. (polymerelektronik.org)
  • Other small viruses, including human parvovirus ( 4 , 5 ) and human papilloma viruses ( 6 ), encode proteins that can cause already dividing cells to arrest at stages of the cell cycle more favorable for virus replication (i.e. (frontiersin.org)
  • Both the Andersen and MOUDI impactors were able to sample live viruses from test aerosols , although the relative recovery rate of MS2 was higher than adenovirus (P (cdc.gov)
  • The results confirm that nonviable cascade impactors are capable of size-separating and detecting aerosolized viruses in the human respirable range, and that MS2 and adenovirus can retain viability after nebulization under experimental conditions. (cdc.gov)
  • The findings cast doubt, however, on the suitability of MS2 as a general surrogate for human and animal viruses. (cdc.gov)
  • Adenoviruses are very common DNA viruses that infect us throughout our lifetime, causing colds, sore throats, bronchitis, diarrhoea or conjunctivitis. (cosmosmagazine.com)
  • Viruses need to enter human cells and use the "machinery" inside the human's cell to live and reproduce. (sspediatrics.com)
  • Adenoviruses are a family of related viruses which cause mild infections in people and which have been converted into gene transfer vectors by blocking their ability to replicate in cells. (ukri.org)
  • The overall public health risk from currently known influenza viruses at the human-animal interface has not changed, and the likelihood of sustained human-to-human transmission of these viruses remains low. (who.int)
  • Human infections with viruses of animal origin are expected at the human-animal interface wherever these viruses circulate in animals. (who.int)
  • Most human cases were exposed to A(H5) viruses through contact with infected poultry or contaminated environments, including live poultry markets. (who.int)
  • Since the viruses continue to be detected in animals and environments, further human cases can be expected. (who.int)
  • 2. What is the likelihood of human-to-human transmission of avian influenza A(H5) viruses? (who.int)
  • Even though small clusters of A(H5) virus infections have been reported previously including those involving healthcare workers, current epidemiological and virological evidence suggests that influenza A(H5) viruses have not acquired the ability of sustained transmission among humans, thus the likelihood is low. (who.int)
  • Human adenoviruses (HAdVs) cause ocular infections. (molvis.org)
  • The authors conclude that a positive Adenoclone test is reliable in the rapid diagnosis of early adenovirus ocular infections. (nih.gov)
  • Adenovirus has been associated with both sporadic and epidemic disease and, with regard to infections among military recruits, who were routinely immunized against types 4 and 7 from 1971 until the cessation of vaccine production in 1996. (medscape.com)
  • Since the first adenovirus was isolated from human adenoid tissue in 1953, some 50 human serotypes have been identified and associated with various syndromes, including upper respiratory tract infections in young children, acute respiratory disease in military recruits, epidemic keratoconjunctivitis, and gastroenteritis. (biomedcentral.com)
  • According to researchers in the U.S. a common virus (adenovirus-36), that causes throat and eye infections may play a part in obesity. (news-medical.net)
  • Adenoviruses, which exist in the wild in humans and typically cause mild infections such as the common cold, have been genetically engineered to express viral antigens found in SARS-CoV-2, usually those of the infamous spike protein that the coronavirus uses to break into human cells. (allianceforscience.org)
  • One study in Finland found ~15% (5% for each group) of rapid virus detection specimens had adenovirus, human metapneumovirus and human bocavirus (a Parvoviridae virus causing respiratory infections identified in 2005). (pediatriceducation.org)
  • Modern engineered vaccines - where a conspicuous bit of the target pathogen is placed like a passenger into the adenovirus chassis - have been used on thousands of individuals in trials to prevent Ebola, malaria, dengue, Marburg and HIV infections. (cosmosmagazine.com)
  • Co-infections of adenovirus species in previously vaccinated patients. (medscape.com)
  • Tabain I, Ljubin-Sternak S, Cepin-Bogovic J, Markovinovic L, Knezovic I, Mlinaric-Galinovic G. Adenovirus Respiratory Infections in Hospitalized Children: Clinical Findings in Relation to Species and Serotypes. (medscape.com)
  • Molecular epidemiology of adenovirus type 4 infections in US military recruits in the postvaccination era (1997-2003). (medscape.com)
  • All human infections caused by a new influenza subtype are required to be reported under the International Health Regulations (IHR, 2005).4 This includes any influenza A virus that has demonstrated the capacity to infect a human and its haemagglutinin gene (or protein) is not a mutated form of those, i.e. (who.int)
  • The UVRI is the largest Government Research Institution in Uganda dealing in health research pertaining to human infections and disease processes associated with or linked to viral etiology and is host to a number of partner organizations including those funded by US CDC, US NIH, MRC-UK and IAVI, as well as national and regional reference laboratories. (lshtm.ac.uk)
  • How Common Is Human Metapneumovirus? (pediatriceducation.org)
  • The laboratory evaluation of a nasal wash specimen done in the emergency room was positive for human metapneumovirus. (pediatriceducation.org)
  • Common viral causes of pneumonia include Respiratory Syncytial virus, Influenza and B, Parainfluenza, Human metapneumovirus and Adenovirus. (pediatriceducation.org)
  • Human metapneumovirus (hMPV) is a single-stranded RNA virus in the Paramyxoviridae family that is closely related to avian metapneumovirus. (pediatriceducation.org)
  • Of interest is the role of adenoviruses as viral vectors in vaccination and in gene therapy.For example, in viral vector vaccines against SARS-CoV2, the vector virus is used to deliver RNA encoding SARS-CoV2 spike protein into target cells. (medscape.com)
  • Almost 200 trials are ongoing of adenovirus-based therapeutics and vaccines. (polymerelektronik.org)
  • Similar immune responses have also been reported in clinical studies that evaluated immunogenicity of mixed schedules with the adenovirus and Modified Vaccinia virus Ankara ( MVA ) Ebola vaccines Footnote 2 , Footnote 3 . (canada.ca)
  • This is a new technology: no adenovirus vector vaccines for other diseases are yet widely available, though vaccines for HIV, influenza, Ebola and malaria using this platform are in clinical trials and an Ebola vaccine has been briefly deployed. (allianceforscience.org)
  • As you can see form this list, these CDC-approved vaccines are deliberately formulated with all sorts of highly toxic and questionable ingredients, including acetone, a toxic solvent, aluminum, artificial coloring chemicals, human blood components, dextrose (probably from GMO corn), cow blood components and a whole lot more. (newstarget.com)
  • Are these vaccines made with African Green Monkey kidney cells and aborted human fetal cells really used in the United States? (newstarget.com)
  • This page from the FDA lists all the vaccines which are "licensed for use in the United States," and it includes the vaccines containing African Green Monkey kidney cells and the WI-38 aborted human fetal cells. (newstarget.com)
  • While this is the first time we've seen international-scale vaccination programs, adenovirus vaccines are not new. (cosmosmagazine.com)
  • MHC class II invariant chain-adjuvanted viral vectored vaccines enhances T cell responses in humans. (ox.ac.uk)
  • Vaccination was well tolerated and enhanced the peak magnitude, breadth, and proliferative capacity of anti-HCV T cell responses compared to non-Ii vaccines in humans. (ox.ac.uk)
  • This study aimed to evaluate the potency, toxicity, and protection of candidate PastoCoAd vaccines as novel mix and match of recombinant adenovirus type 5 (rAd5) containing the full-length spike protein (rAd5-S), rAd5 containing the receptor-binding domain of S protein and nucleoprotein (rAd5 RBD-N), and SOBERANA dimeric RBD protein of SARS-CoV-2. (bvsalud.org)
  • A downside, however, is that so far, no DNA or RNA vaccines have been licensed for human use, which may cause more hurdles with regulatory approval. (gavi.org)
  • The most common viral pathogens recovered from hospitalized patients admitted with CAP include human rhinovirus and influenza . (medscape.com)
  • Because of its acid sensitivity and low optimum growth temperature (33°C), EV68 shares characteristics with human rhinovirus (HRV) (3,6) and is genetically and antigenically similar to HRV 87 (6,7). (blogspot.com)
  • Our understanding of adenovirus (Ad) biology is largely extrapolated from human species C Ad5. (elsevier.com)
  • Human species D adenovirus hexon capsid protein mediates cell entry through a direct interaction with CD46. (amedeo.com)
  • Species of the genus MASTADENOVIRUS , causing a wide range of diseases in humans. (bvsalud.org)
  • Serotypes (named with Arabic numbers) have been grouped into species designated Human adenovirus A-G. (bvsalud.org)
  • Like other animals, why humans don't have species & breeds? (stackexchange.com)
  • Enterovirus 68 (EV68) belongs to the family Picornaviridae, genus Enterovirus, and species Human enterovirus D (1). (blogspot.com)
  • Severe acute respiratory syndrome-related coronavirus ( SARSr-CoV or SARS-CoV ) [note 1] is a species of virus consisting of many known strains phylogenetically related to severe acute respiratory syndrome coronavirus 1 (SARS-CoV-1) that have been shown to possess the capability to infect humans , bats , and certain other mammals . (wikizero.com)
  • [16] Only recently have strains of SARS-related coronavirus been observed to have evolved into having been able to make the cross-species jump from bats to humans, as in the case of the strains SARS-CoV-1 and SARS-CoV-2 . (wikizero.com)
  • [17] [4] Both of these strains descended from a single ancestor but made the cross-species jump into humans separately. (wikizero.com)
  • Diagnostic Accuracy of Four Commercial Triplex Immunochromatographic Tests for Rapid Detection of Rotavirus, Adenovirus, and Norovirus in Human Stool Samples. (bvsalud.org)
  • Fecal specimens submitted for routine bacterial culture through a health maintenance organization in Georgia, USA, were tested with molecular diagnostic assays for norovirus, rotavirus, astrovirus, sapovirus, and adenovirus. (cdc.gov)
  • Rotavirus/Adenovirus/Norovi. (innovitaivd.com)
  • With advances in molecular biology informing recent developments in vaccinology, gene therapy, and other fields of medicine, human adenoviruses (HAdVs) have been of primary importance in medical research. (polymerelektronik.org)
  • Other zoonotic HAdVs are also being reported, and some researchers suggest repeated human-animal interspecies leaps occur with several recombinations. (polymerelektronik.org)
  • Human adenoviruses (HAdVs) are highly contagious pathogens of clinical importance, especially among the pediatric population. (honours.eu)
  • Human adenoviruses (HAdVs) are common pathogens that cause several diseases, such as pneumonia, acute gastroenteritis, and epidemic keratoconjunctivitis ( 1 ). (cdc.gov)
  • Downmodulation of E1A protein expression as a novel strategy to design cancer-selective adenoviruses. (semanticscholar.org)
  • It is concluded that the level of E1A protein is a critical determinant of oncolytic phenotype and a completely novel strategy for the design and construction of conditionally replicative adenoviruses is proposed. (semanticscholar.org)
  • Hexon protein is a major coat protein of adenoviruses. (novusbio.com)
  • Adenoviruses capsids have three principal protein components: the hexon, the penton, and the fiber. (novusbio.com)
  • A purified adenovirus 289-amino-acid E1A protein activates RNA polymerase III transcription in vitro and alters TFIIIC. (microbiologyresearch.org)
  • Activation of transcription factor IIIC by adenovirus E1A protein. (microbiologyresearch.org)
  • The expression of human vasohibin-1 protein was detected by immunohistochemistry and western blotting at 5, 8, and 14 days after injection. (elsevier.com)
  • Human vasohibin-1 protein was detected at the injection sites on day 3 after corneal burn and was highly expressed in the central subepithelial stroma and colocalized with neovascularized vessels within the alkali-treated cornea on day 6. (elsevier.com)
  • Human adenovirus E4orf4 protein is toxic in human tumor cells. (cell-signaling-pathways.com)
  • The DNA-binding domain of human c-Abl tyrosine kinase promotes the interaction of a HMG chromosomal protein with DNA. (nih.gov)
  • The CR2 region of the adenovirus E1A protein also interacts with pRB, E2F is released and the virus replicates. (medicaltrend.org)
  • Adenovirus E2 promoter can be divided into early and late promoters, used to regulate the E2 transcription unit, the late E2 promoter region contains Y-box, the binding site of transcription factor YB-1 (Y-box binding protein-1). (medicaltrend.org)
  • The E1B region of the adenovirus genome encodes a 55kD protein (E1B55K), which binds and inactivates TP53. (medicaltrend.org)
  • Evaluation of on-line high-performance size-exclusion chromatography, differential refractometry, and multi-angle laser light scattering analysis for the monitoring of the oligomeric state of human immunodeficiency virus vaccine protein antigen. (nchu.edu.tw)
  • Humans do not produce Vitamin C due to a mutation in the GULO (gulonolactone oxidase) gene, which results in the inability to synthesize the protein. (stackexchange.com)
  • In the case of COVID-19, the antigen is typically the characteristic spike protein found on the surface of the virus, which it normally uses to help it invade human cells. (gavi.org)
  • Once this genetic material gets into human cells, it uses our cells' protein factories to make the antigen that will trigger an immune response. (gavi.org)
  • BACKGROUND/AIMS Replication deficient recombinant adenoviruses represent an efficient means of transferring genes in vivo into a wide variety of dividing and quiescent cells from many different organs. (bmj.com)
  • The prospects of using recombinant adenoviruses for gene delivery into epithelial and subepithelial cells of the normal and inflamed colon are here analysed. (bmj.com)
  • CONCLUSIONS Local administration of recombinant adenoviruses with normal or modified fibre structure could provide a new reliable method for targeted gene expression in the inflamed colon. (bmj.com)
  • Information from these notifications is critical to inform risk assessments for influenza at the human-animal interface. (who.int)
  • As a group the pathogens ETEC, Shigella and adenovirus were detected more frequently (10/22) during the wet season than the dry season (2/20). (researchgate.net)
  • Other recently identified pathogens include the enteric adenoviruses, calicivirus, astrovirus, and the Norwalk family of agents. (cdc.gov)
  • Adenovirus serotype 14 (Ad14) is a rarely reported but emerging serotype of adenovirus that can cause severe and sometimes fatal respiratory illness in patients of all ages, including healthy young adults. (cdc.gov)
  • Human adenoviruses, such as serotype 5 (Ad5), encode several proteins that can perturb cellular mechanisms that regulate cell cycle progression and apoptosis, as well as those that mediate mRNA production and translation. (biomedcentral.com)
  • The most common member of the adenovirus family used is adenovirus serotype 5 (Ad5). (ukri.org)
  • We made a recent discovery that when injected into the bloodstream adenovirus serotype 5 becomes coated in one of our own proteins in the blood called a coagulation factor. (ukri.org)
  • Adenoviral vectors, particularly human serotype 5 (of 58 individual serotypes), are widely used for gene therapy due to versatile tropism, large transgene capacity and ability to achieve efficient transgene without risk of insertional mutagenesis. (ukri.org)
  • Our aim was to examine a population of children who are at risk from P. falciparum malaria for neutralizing immunity to replication-deficient recombinant chimpanzee adenovirus 63 vector (AdC63), compared to human adenovirus 5 vector (AdHu5). (ox.ac.uk)
  • Induction of integrated adenovirus E1A and E1B genes in transformed human cells by phorbol ester tumor promoters. (microbiologyresearch.org)
  • This allows the adenoviral vector to be used for gene therapy (the use of genes to treat human disease). (ukri.org)
  • In fact, adenoviruses are one of the most common vectors in use in gene therapy clinical trials because they are easy to work with, safe and are efficient at delivering genes to cells and tissues in the body. (ukri.org)
  • Dmitry Kazansky: Human blood is taken, lymphocytes are separated from other cells, then artificial viral constructs are used to introduce the necessary genes into lymphocytes. (historical-facts.com)
  • Hexon antigen of human adenovirus. (novusbio.com)
  • Adenovirus hexon specific. (novusbio.com)
  • ETEC), ipaH (Shigella) and 40/41 hexon (adenovirus) were detected using quantitative polymerase chain reaction (qPCR). (researchgate.net)
  • four (8%) did not test positive for adenovirus. (cdc.gov)
  • As of May 5, 2022, CDC and state partners are investigating 109 children with hepatitis of unknown origin across 25 states and territories, more than half of whom have tested positive for adenovirus with more than 90% hospitalized, 14% with liver transplants, and five deaths under investigation. (cdc.gov)
  • abstract = "Objective: This study was undertaken to develop a representative murine model for human leiomyoma. (elsevier.com)
  • Type 5 of Adenovirus infects mainly epithelial cells and causes a mild pathology with flulike symptoms and is mainly used to study the molecular biology of adenoviruses. (semanticscholar.org)
  • Human Molecular Genetics. (elsevier.com)
  • Two strains of the virus have caused outbreaks of severe respiratory diseases in humans: severe acute respiratory syndrome coronavirus 1 (SARS-CoV or SARS-CoV-1), which caused the 2002-2004 outbreak of severe acute respiratory syndrome (SARS), and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which is causing the ongoing pandemic of COVID-19 . (wikizero.com)
  • The genome of adenovirus is well known and can be modified with relative ease to induce lysis or cytotoxicity of a specified cell line without affecting others. (medscape.com)
  • Respiratory Illness Associated With Emergent Human Adenovirus Genome Type 7d, New Jersey, 2016-2017. (medscape.com)
  • Adenovirus DNA : the viral genome and its expression / edited by Walter Doerfler. (who.int)
  • The infectious cycle of subgroup C human adenovirus, such as adenovirus type 5 (Ad5), in permissive cells in culture is characterized by a strict temporal program of viral gene expression that culminates in production of large quantities of viral DNA and structural proteins. (biomedcentral.com)
  • Synthesis of progeny DNA genomes in cells infected by human subgroup C adenoviruses leads to several changes in viral gene expression. (princeton.edu)
  • Expression of the chloramphenicol acetyl transferase gene in human cells under the control of early adenovirus subgroup C promoters: effect of E1A gene products from other subgroups on gene expression. (nih.gov)
  • Transverse chest CT images demonstrated in- We identified a case of fatal acute respiratory disease creased areas of patchy shadows and consolidation in both from household transmission of human adenovirus type lungs compared to CT images from April 22, indicative of 55 (HAdV-55) in Anhui Province, China. (cdc.gov)
  • Human adenovirus type 8 (HAdV-8) is one of the major serotypes isolated from patients with EKC. (molvis.org)
  • Human adenovirus (HAdV) is a major cause of viral conjunctivitis. (who.int)
  • We report a novel human adenovirus D (HAdV-65) isolated from feces of 4 children in Bangladesh who had acute gastroenteritis. (cdc.gov)
  • Phylogenetic trees of a novel human adenovirus (HAdV-65), 3 strains (DC 11, DC 253, and DC 303), and other HAdV-D reference strains. (cdc.gov)
  • It was in 2020 that the first adenovirus vaccine gained approval, against the Ebola virus, for use in exceptional circumstances. (polymerelektronik.org)
  • Adenoviruses are not the only viral vectors that can be used: pharmaceutical giant Merck says it is working on a potential COVID vaccine using an engineered vesicular stomatis virus , previously used successfully in its Ebola vaccine. (allianceforscience.org)
  • Oncolytic adenovirus is a type of conditionally replicating adenovirus. (medicaltrend.org)
  • In recent years, oncolytic adenovirus therapy has become more and more important as a new type of treatment for various cancers. (medicaltrend.org)
  • Telomelysin (OBP-301) is a gene-modified oncolytic adenovirus that selectively replicates in cancer cells by introducing human telomerase reverse transcriptase (hTERT) promoter. (secunderabadchronicle.in)
  • Recently, this strain was found to have acquired gain-of-function mutations adding a key replication motif, nuclear factor 1 (NF-1), absent in the parental E4 strain but required for efficient replication within human host cells. (polymerelektronik.org)
  • Tumor promoter alters the temporal program of adenovirus replication in human cells. (microbiologyresearch.org)
  • E1 deleted adenoviruses are considered to be replication-defective and are used as shuttle vectors in gene therapy or vaccination for gene therapy and vaccine immunization. (medicaltrend.org)
  • Adenovirus vectors generally have four strategies to achieve conditional replication: E1A specific promoter regulation, E1A CR2 deletion, E1A 13S CR3 deletion and E1B-55K deletion. (medicaltrend.org)
  • Since E1A 13S is essential for the transport of YB-1 from the cytoplasm to the nucleus, adenoviruses lacking E1A13S expression have replication defects in normal cells. (medicaltrend.org)
  • The replication of adenovirus in cells activates the TP53-mediated apoptosis pathway. (medicaltrend.org)
  • Finally, AdZ.F(pk7) adenoviruses with modified fibre structure produced 10- to 40-fold higher reporter gene activity in spleen T cells and lamina propria mononuclear cells of colitic mice compared with standard AdCMVβGal vectors. (bmj.com)
  • These experiments will help our further understanding of adenoviruses and ultimately lead to more optimal and safe vectors for all adenoviral gene therapy approaches. (ukri.org)
  • We now assess this strategy in humans, using chimpanzee adenovirus 3 and modified vaccinia Ankara vectors encoding human Ii fused to the nonstructural (NS) antigens of hepatitis C virus (HCV) in a heterologous prime/boost regimen. (ox.ac.uk)
  • Furthermore, funding of the actual clinical trial was not guaranteed even vectors have been produced and certified for human use at significant cost. (swfinstitute.org)
  • Nasopharyngeal aspirates were tested using direct immunofluorescence with an adenovirus specific monoclonal antibody (Dako Imogen, USA). (bmj.com)
  • In comparison with the Ad14 patients, among the 12 adenovirus non-type 14 patients (i.e., all but three) whose medical charts were reviewed, nine (75%) were male. (cdc.gov)
  • Mechanism of host cell restriction in African green monkey kidney cells abortively infected with human adenovirus type 2. (wikidata.org)
  • Outbreak of adenovirus type 30 in a neonatal intensive care unit. (medscape.com)
  • the five specimens that could be typed were all adenovirus type 41. (cdc.gov)
  • Modulation of drug cytotoxicity by reintroduction of wild-type p53 gene (Ad5CMV-p53) in human pancreatic cancer. (irbbarcelona.org)
  • Ethics and compliance in research covers a broad range of activity from general guidelines about conducting research responsibly to specific regulations governing a type of research (e.g., human subjects research, export controls, conflict of interest). (umich.edu)
  • One type of virus that has often been used as a vector is adenovirus, which causes the common cold. (gavi.org)
  • Cellular damage produced by conditions generating oxidative stress have far-reaching implications in human disease that encompass, but are not restricted to aging, cardiovascular disease, type 2 diabetes, airway inflammation/asthma, cancer, and metabolic syndrome including visceral obesity, insulin resistance, fatty liver disease, and dyslipidemia. (luciolepharma.com)
  • The therapeutic efficacy of Adenovirus type 7 vaccine live can be decreased when used in combination with TMC-310911. (drugbank.com)
  • Adenoviruses are immunogenic and elicit strong innate and adaptive immune responses. (medscape.com)
  • These engineered adenoviruses, when put into a vaccine, trigger an immune response in the human body, protecting against COVID-19. (allianceforscience.org)
  • TNFSF14 can act as an immune adjuvant and enhance the immunogenicity of the human papillomavirus (HPV) DNA vaccine when the host is infected with HPV. (bvsalud.org)
  • tetanus immune globulin (TIG) decreases effects of adenovirus types 4 and 7 live, oral by pharmacodynamic antagonism. (medscape.com)
  • Importance of enteric adenoviruses 40 and 41 in acute gastroenteritis in infants and young children. (medscape.com)
  • Accelerated onset of viral transcription in adenovirus-infected HeLa cells treated with the tumor promoter 12- O - tetradecanoyl-phorbol-13-acetate. (microbiologyresearch.org)
  • METHODS An E1/E3 deleted recombinant adenovirus (denoted AdCMVβGal) and an adenovirus with modified fibre structure (denoted AdZ.F(pk7)) both expressing the bacterial lacZ gene under the control of a human cytomegalovirus promoter were used for reporter gene expression in vitro and in vivo. (bmj.com)
  • early detection at the human-animal interface will provide earlier warning. (onehealthinitiative.com)
  • Effect of disease state on ionization during bioanalysis of MK-7009, a selective HCV NS3/NS4 protease inhibitor, in human plasma and human Tween-treated urine by high-performance liquid chromatography with tandem mass spectrometric detection. (nchu.edu.tw)
  • Nucleic acid amplification testing (NAAT), such as polymerase chain reaction (PCR), is preferred for adenovirus detection (currently not available for FFPE liver biopsy or native liver explant). (cdc.gov)
  • Interaction of a simian papovavirus and adenoviruses. (wikidata.org)
  • In 1993 it came to be used for therapeutic CFTR gene delivery in cystic fibrosis, the first effective human gene to be used in vivo for human gene therapy. (polymerelektronik.org)
  • Modified adenovirus has also been used to treat cancer and for gene therapy. (cosmosmagazine.com)
  • Human gene therapy. (stackexchange.com)
  • These data demonstrate shared as well as divergent life cycles in these genetically distant human adenoviruses. (elsevier.com)
  • A big-name corporate player is Johnson & Johnson, via its subsidiary Janssen, which uses a genetically modified human adenovirus technology it calls AdVac. (allianceforscience.org)
  • Postmortem tracheal and gastric swabs from the infant were sent to the Wadsworth Center laboratory of the New York State Department of Health, where adenovirus was detected by polymerase chain reaction (PCR). (cdc.gov)
  • Enhancement of RNA polymerase III transcription by E1A gene product of adenovirus. (microbiologyresearch.org)
  • Human DNA Polymerase η Is Pre-Aligned. (neb.com)
  • To extend application of TLR5-targeted anticancer immunotherapy to tumors that do not naturally express TLR5, we created an adenovirus-based vector for intratumor delivery, named Mobilan that drives expression of self-activating TLR5 signaling cassette comprising of human TLR5 and a secreted derivative of Salmonella flagellin structurally analogous to a clinical stage TLR5 agonist, entolimod. (nature.com)
  • However, existing immunity to the adenovirus vector results in antibody-mediated neutralization of the vaccine vector, and reduced vaccine immunogenicity. (ox.ac.uk)
  • The Chinese company CanSino Biologics - the medical science arm of the People's Liberation Army, no less - has completed Phase 1 trials with an adenovirus vector vaccine called Ad5-nCoV. (allianceforscience.org)
  • Nonhuman primates and humans are probably the main reservoirs of the virus, and anthroponotic transmission (man-vector-man) occurs during outbreaks. (bvsalud.org)
  • Adenovirus, a DNA virus, was first isolated in the 1950s in adenoid tissue-derived cell cultures, hence the name. (medscape.com)
  • An extremely hardy virus, adenovirus is ubiquitous in human and animal populations, survives long periods outside a host, and is endemic throughout the year. (medscape.com)
  • Adenovirus is a common respiratory virus in children and is known to cause acute haemorrhagic cystitis, particularly in the immunosuppressed. (bmj.com)
  • A case of adenovirus and herpes simplex virus has been described in which granular depositions of adeno and herpes simplex viral antigens were detected in the glomerular mesangium. (bmj.com)
  • The scientists from Pennington Biomedical Centre at Louisiana State University, conducted laboratory tests on human fatty tissue and found that the virus triggered changes in the tissue that left people with more, and larger, fat cells than people who were not infected. (news-medical.net)
  • Dr. Dhurandhar says the virus adenovirus-36 or Ad-36, caused animals to pack on the pounds in earlier lab experiments and the animals accumulated far more fat. (news-medical.net)
  • ACAM2000 is provided as a lyophilized preparation of purified live virus containing the following non-active excipients: 6-8 mM HEPES (pH 6.5-7.5), 2% human serum albumin USP, 0.5 - 0.7% sodium chloride USP, 5% mannitol USP, and trace amounts of neomycin and polymyxin B. (newstarget.com)
  • The deletion of adenovirus E1A CR2 prevents the combination of E1A and pRB, and the virus cannot release E2F in normal cells, nor can it replicate. (medicaltrend.org)
  • The Zika virus is transmitted to humans through the bite of an infected mosquito of the genus Aedes , mainly Aedes aegypti . (bvsalud.org)
  • 4 The association with haematuria has also been seen in reports of positive adenovirus immunofluorescence on throat swabs in patients with glomerulonephritis. (bmj.com)
  • The adenovirus early region 1A (E1A) proteins were described originally as immortalizing oncoproteins that altered transcription in rodent cells. (semanticscholar.org)
  • Surprisingly, the 243-amino-acid form of adenovirus-5 E1A was found subsequently to reverse-transform many human tumour cells. (semanticscholar.org)
  • Adenovirus E1A proteins are closely associated with chromatin in productively infected and transformed cells. (semanticscholar.org)
  • human being non-small-cell lung carcinoma cells (ATCC CRL-5803) had been cultured under regular conditions as referred to previously (53 57 Some research also used H1299/HA-Bα cells that stably communicate rat HA-Bα subunit and which were prepared by regular strategies using coselection with neomycin. (cell-signaling-pathways.com)
  • Diagnosis of adenovirus was made by culture with cyanmologous monkey kidney cells as the primary cell line and human embryonic fibroblasts. (bmj.com)
  • Some of the arguments backing this assertion included wildly uninformed opinions such as, "there's no way the CDC would approve monkey kidney cells being injected into humans. (newstarget.com)
  • Adenoviruses lacking E1B-55K will not be able to replicate effectively in cells with normal TP53 function, while they can replicate and lyse killer cells in large numbers in cells with TP53 gene mutation, deletion or inactivation. (medicaltrend.org)
  • Very high frequencies of HCV-specific T cells were elicited in humans. (ox.ac.uk)
  • When synthetic mRNA enters the human patient, the material fuses to cells and cell's molecules start to decode the genomic sequence to build the spike proteins. (swfinstitute.org)
  • Outbreaks of adenovirus-associated respiratory illness on five college campuses in the United States. (medscape.com)
  • Identification and control of nosocomial adenovirus keratoconjunctivitis in an ophthalmic department. (medscape.com)
  • Adenovirus is known to be oncogenic in rodents but not in humans. (medscape.com)
  • A live oral vaccine against adenovirus types 4 and 7 was approved for use in this population by the US Food and Drug Administration (FDA) in 2011, and subsequent incidence of acute respiratory disease declined. (medscape.com)
  • Adenovirus causes 5-8% of respiratory disease in infants and has many other clinical manifestations. (bmj.com)
  • Adenovirus-mediated wt-p16 reintroduction induces cell cycle arrest or apoptosis in pancreatic cancer. (irbbarcelona.org)
  • Clinicians are recommended to consider adenovirus testing for patients with hepatitis of unknown etiology and to report such cases to their state or jurisdictional public health authorities. (cdc.gov)