Adenoviruses, Human: Species of the genus MASTADENOVIRUS, causing a wide range of diseases in humans. Infections are mostly asymptomatic, but can be associated with diseases of the respiratory, ocular, and gastrointestinal systems. Serotypes (named with Arabic numbers) have been grouped into species designated Human adenovirus A-F.Adenoviridae: A family of non-enveloped viruses infecting mammals (MASTADENOVIRUS) and birds (AVIADENOVIRUS) or both (ATADENOVIRUS). Infections may be asymptomatic or result in a variety of diseases.Adenovirus Infections, Human: Respiratory and conjunctival infections caused by 33 identified serotypes of human adenoviruses.Adenovirus E1A Proteins: Proteins transcribed from the E1A genome region of ADENOVIRUSES which are involved in positive regulation of transcription of the early genes of host infection.Adenoviridae Infections: Virus diseases caused by the ADENOVIRIDAE.Adenovirus Early Proteins: Proteins encoded by adenoviruses that are synthesized prior to, and in the absence of, viral DNA replication. The proteins are involved in both positive and negative regulation of expression in viral and cellular genes, and also affect the stability of viral mRNA. Some are also involved in oncogenic transformation.Adenovirus E1B Proteins: Proteins transcribed from the E1B region of ADENOVIRUSES which are involved in regulation of the levels of early and late viral gene expression.Adenovirus E3 Proteins: Proteins transcribed from the E3 region of ADENOVIRUSES but not essential for viral replication. The E3 19K protein mediates adenovirus persistence by reducing the expression of class I major histocompatibility complex antigens on the surface of infected cells.Adenovirus E4 Proteins: Proteins transcribed from the E4 region of ADENOVIRUSES. The E4 19K protein transactivates transcription of the adenovirus E2F protein and complexes with it.Adenovirus E1 Proteins: The very first viral gene products synthesized after cells are infected with adenovirus. The E1 region of the genome has been divided into two major transcriptional units, E1A and E1B, each expressing proteins of the same name (ADENOVIRUS E1A PROTEINS and ADENOVIRUS E1B PROTEINS).Genetic Vectors: DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.Adenoviruses, Canine: Species of the genus MASTADENOVIRUS that causes fever, edema, vomiting, and diarrhea in dogs and encephalitis in foxes. Epizootics have also been caused in bears, wolves, coyotes, and skunks. The official species name is Canine adenovirus and it contains two serotypes.Adenovirus E2 Proteins: Proteins transcribed from the E2 region of ADENOVIRUSES. Several of these are required for viral DNA replication.Mastadenovirus: A genus of ADENOVIRIDAE that infects MAMMALS including humans and causes a wide range of diseases. The type species is Human adenovirus C (see ADENOVIRUSES, HUMAN).Adenoviruses, Porcine: Species of the genus MASTADENOVIRUS, causing neurological disease in pigs.Aviadenovirus: A genus of ADENOVIRIDAE that infects birds. The type species is FOWL ADENOVIRUS A.Genetic Therapy: Techniques and strategies which include the use of coding sequences and other conventional or radical means to transform or modify cells for the purpose of treating or reversing disease conditions.Fowl adenovirus A: The type species of the genus AVIADENOVIRUS, family ADENOVIRIDAE, an oncogenic virus of birds. This is also called CELO virus for chick embryo lethal orphan virus.DNA, Viral: Deoxyribonucleic acid that makes up the genetic material of viruses.Gene Transfer Techniques: The introduction of functional (usually cloned) GENES into cells. A variety of techniques and naturally occurring processes are used for the gene transfer such as cell hybridization, LIPOSOMES or microcell-mediated gene transfer, ELECTROPORATION, chromosome-mediated gene transfer, TRANSFECTION, and GENETIC TRANSDUCTION. Gene transfer may result in genetically transformed cells and individual organisms.Capsid Proteins: Proteins that form the CAPSID of VIRUSES.HeLa Cells: The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Virus Replication: The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.Receptors, Virus: Specific molecular components of the cell capable of recognizing and interacting with a virus, and which, after binding it, are capable of generating some signal that initiates the chain of events leading to the biological response.Oncolytic Virotherapy: Use of attenuated VIRUSES as ANTINEOPLASTIC AGENTS to selectively kill CANCER cells.Oncolytic Viruses: Tumor-selective, replication competent VIRUSES that have antineoplastic effects. This is achieved by producing cytotoxicity-enhancing proteins and/or eliciting an antitumor immune response. They are genetically engineered so that they can replicate in CANCER cells but not in normal cells, and are used in ONCOLYTIC VIROTHERAPY.Keratoconjunctivitis: Simultaneous inflammation of the cornea and conjunctiva.Transduction, Genetic: The transfer of bacterial DNA by phages from an infected bacterium to another bacterium. This also refers to the transfer of genes into eukaryotic cells by viruses. This naturally occurring process is routinely employed as a GENE TRANSFER TECHNIQUE.Viral Proteins: Proteins found in any species of virus.Genes, Viral: The functional hereditary units of VIRUSES.Oncogene Proteins, Viral: Products of viral oncogenes, most commonly retroviral oncogenes. They usually have transforming and often protein kinase activities.Conjunctivitis, Viral: Inflammation, often mild, of the conjunctiva caused by a variety of viral agents. Conjunctival involvement may be part of a systemic infection.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Adenovirus Vaccines: Vaccines used to prevent infection by any virus from the family ADENOVIRIDAE.Capsid: The outer protein protective shell of a virus, which protects the viral nucleic acid.Cell Transformation, Viral: An inheritable change in cells manifested by changes in cell division and growth and alterations in cell surface properties. It is induced by infection with a transforming virus.Transgenes: Genes that are introduced into an organism using GENE TRANSFER TECHNIQUES.Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Transfection: The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.Cytopathogenic Effect, Viral: Visible morphologic changes in cells infected with viruses. It includes shutdown of cellular RNA and protein synthesis, cell fusion, release of lysosomal enzymes, changes in cell membrane permeability, diffuse changes in intracellular structures, presence of viral inclusion bodies, and chromosomal aberrations. It excludes malignant transformation, which is CELL TRANSFORMATION, VIRAL. Viral cytopathogenic effects provide a valuable method for identifying and classifying the infecting viruses.Helper Viruses: Viruses which enable defective viruses to replicate or to form a protein coat by complementing the missing gene function of the defective (satellite) virus. Helper and satellite may be of the same or different genus.Defective Viruses: Viruses which lack a complete genome so that they cannot completely replicate or cannot form a protein coat. Some are host-dependent defectives, meaning they can replicate only in cell systems which provide the particular genetic function which they lack. Others, called SATELLITE VIRUSES, are able to replicate only when their genetic defect is complemented by a helper virus.Promoter Regions, Genetic: DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.beta-Galactosidase: A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.RNA, Viral: Ribonucleic acid that makes up the genetic material of viruses.Gene Expression Regulation, Viral: Any of the processes by which cytoplasmic factors influence the differential control of gene action in viruses.Gene Expression: The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.Enterovirus: A genus of the family PICORNAVIRIDAE whose members preferentially inhabit the intestinal tract of a variety of hosts. The genus contains many species. Newly described members of human enteroviruses are assigned continuous numbers with the species designated "human enterovirus".DNA Replication: The process by which a DNA molecule is duplicated.ConjunctivitisPlasmids: Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Tumor Cells, Cultured: Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Atadenovirus: A genus of ADENOVIRIDAE that comprises viruses of several species of MAMMALS and BIRDS. The type species is Ovine adenovirus D.Simian virus 40: A species of POLYOMAVIRUS originally isolated from Rhesus monkey kidney tissue. It produces malignancy in human and newborn hamster kidney cell cultures.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.DNA-Binding Proteins: Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.Mice, Nude: Mutant mice homozygous for the recessive gene "nude" which fail to develop a thymus. They are useful in tumor studies and studies on immune responses.Virus Cultivation: Process of growing viruses in live animals, plants, or cultured cells.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Genome, Viral: The complete genetic complement contained in a DNA or RNA molecule in a virus.Mice, Inbred BALB CCricetinae: A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.Recombinant Fusion Proteins: Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.Cell Line, Tumor: A cell line derived from cultured tumor cells.Respiratory Tract Infections: Invasion of the host RESPIRATORY SYSTEM by microorganisms, usually leading to pathological processes or diseases.Recombination, Genetic: Production of new arrangements of DNA by various mechanisms such as assortment and segregation, CROSSING OVER; GENE CONVERSION; GENETIC TRANSFORMATION; GENETIC CONJUGATION; GENETIC TRANSDUCTION; or mixed infection of viruses.Antibodies, Viral: Immunoglobulins produced in response to VIRAL ANTIGENS.Viruses: Minute infectious agents whose genomes are composed of DNA or RNA, but not both. They are characterized by a lack of independent metabolism and the inability to replicate outside living host cells.Cell Nucleus: Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)Antigens, Viral, Tumor: Those proteins recognized by antibodies from serum of animals bearing tumors induced by viruses; these proteins are presumably coded for by the nucleic acids of the same viruses that caused the neoplastic transformation.Transcription Factors: Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.Serotyping: Process of determining and distinguishing species of bacteria or viruses based on antigens they share.Inclusion Bodies, Viral: An area showing altered staining behavior in the nucleus or cytoplasm of a virus-infected cell. Some inclusion bodies represent "virus factories" in which viral nucleic acid or protein is being synthesized; others are merely artifacts of fixation and staining. One example, Negri bodies, are found in the cytoplasm or processes of nerve cells in animals that have died from rabies.DNA Restriction Enzymes: Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.Pneumonia, Viral: Inflammation of the lung parenchyma that is caused by a viral infection.Haplorhini: A suborder of PRIMATES consisting of six families: CEBIDAE (some New World monkeys), ATELIDAE (some New World monkeys), CERCOPITHECIDAE (Old World monkeys), HYLOBATIDAE (gibbons and siamangs), CALLITRICHINAE (marmosets and tamarins), and HOMINIDAE (humans and great apes).Antigens, Viral: Substances elaborated by viruses that have antigenic activity.Nucleic Acid Hybridization: Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)Antigens, CD46: A ubiquitously expressed complement receptor that binds COMPLEMENT C3B and COMPLEMENT C4B and serves as a cofactor for their inactivation. CD46 also interacts with a wide variety of pathogens and mediates immune response.Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.Cell Line, Transformed: Eukaryotic cell line obtained in a quiescent or stationary phase which undergoes conversion to a state of unregulated growth in culture, resembling an in vitro tumor. It occurs spontaneously or through interaction with viruses, oncogenes, radiation, or drugs/chemicals.Dependovirus: A genus of the family PARVOVIRIDAE, subfamily PARVOVIRINAE, which are dependent on a coinfection with helper adenoviruses or herpesviruses for their efficient replication. The type species is Adeno-associated virus 2.Viral Vaccines: Suspensions of attenuated or killed viruses administered for the prevention or treatment of infectious viral disease.Gene Expression Regulation: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.Gastroenteritis: INFLAMMATION of any segment of the GASTROINTESTINAL TRACT from ESOPHAGUS to RECTUM. Causes of gastroenteritis are many including genetic, infection, HYPERSENSITIVITY, drug effects, and CANCER.Mice, Inbred C57BLKB Cells: This line KB is now known to be a subline of the ubiquitous KERATIN-forming tumor cell line HeLa. It was originally thought to be derived from an epidermal carcinoma of the mouth, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via contamination by HELA CELLS. The cells are positive for keratin by immunoperoxidase staining. KB cells have been reported to contain human papillomavirus18 (HPV-18) sequences.DNA, Recombinant: Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected.Oncogenic Viruses: Viruses that produce tumors.Tropism: The directional growth of an organism in response to an external stimulus such as light, touch, or gravity. Growth towards the stimulus is a positive tropism; growth away from the stimulus is a negative tropism. (From Concise Dictionary of Biology, 1990)Injections, Intralesional: Injections introduced directly into localized lesions.Thymidine Kinase: An enzyme that catalyzes the conversion of ATP and thymidine to ADP and thymidine 5'-phosphate. Deoxyuridine can also act as an acceptor and dGTP as a donor. (From Enzyme Nomenclature, 1992) EC 2.7.1.21.Xenograft Model Antitumor Assays: In vivo methods of screening investigative anticancer drugs, biologic response modifiers or radiotherapies. Human tumor tissue or cells are transplanted into mice or rats followed by tumor treatment regimens. A variety of outcomes are monitored to assess antitumor effectiveness.Green Fluorescent Proteins: Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH.Lac Operon: The genetic unit consisting of three structural genes, an operator and a regulatory gene. The regulatory gene controls the synthesis of the three structural genes: BETA-GALACTOSIDASE and beta-galactoside permease (involved with the metabolism of lactose), and beta-thiogalactoside acetyltransferase.Sigmodontinae: A subfamily of the family MURIDAE comprised of 69 genera. New World mice and rats are included in this subfamily.Apoptosis: One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Hybridization, Genetic: The genetic process of crossbreeding between genetically dissimilar parents to produce a hybrid.Neutralization Tests: The measurement of infection-blocking titer of ANTISERA by testing a series of dilutions for a given virus-antiserum interaction end-point, which is generally the dilution at which tissue cultures inoculated with the serum-virus mixtures demonstrate cytopathology (CPE) or the dilution at which 50% of test animals injected with serum-virus mixtures show infectivity (ID50) or die (LD50).Vaccines, Synthetic: Small synthetic peptides that mimic surface antigens of pathogens and are immunogenic, or vaccines manufactured with the aid of recombinant DNA techniques. The latter vaccines may also be whole viruses whose nucleic acids have been modified.Cell Transformation, Neoplastic: Cell changes manifested by escape from control mechanisms, increased growth potential, alterations in the cell surface, karyotypic abnormalities, morphological and biochemical deviations from the norm, and other attributes conferring the ability to invade, metastasize, and kill.Kidney: Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Mouth Neoplasms: Tumors or cancer of the MOUTH.Time Factors: Elements of limited time intervals, contributing to particular results or situations.Nuclear Proteins: Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.Virus Diseases: A general term for diseases produced by viruses.Feces: Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.NFI Transcription Factors: Transcription factors that were originally identified as site-specific DNA-binding proteins essential for DNA REPLICATION by ADENOVIRUSES. They play important roles in MAMMARY GLAND function and development.Neoplasms, Experimental: Experimentally induced new abnormal growth of TISSUES in animals to provide models for studying human neoplasms.Culture Techniques: Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.Satellite Viruses: Defective viruses which can multiply only by association with a helper virus which complements the defective gene. Satellite viruses may be associated with certain plant viruses, animal viruses, or bacteriophages. They differ from satellite RNA; (RNA, SATELLITE) in that satellite viruses encode their own coat protein.Molecular Weight: The sum of the weight of all the atoms in a molecule.Microscopy, Electron: Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.DNA Primers: Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.Tumor Suppressor Protein p53: Nuclear phosphoprotein encoded by the p53 gene (GENES, P53) whose normal function is to control CELL PROLIFERATION and APOPTOSIS. A mutant or absent p53 protein has been found in LEUKEMIA; OSTEOSARCOMA; LUNG CANCER; and COLORECTAL CANCER.Virion: The infective system of a virus, composed of the viral genome, a protein core, and a protein coat called a capsid, which may be naked or enclosed in a lipoprotein envelope called the peplos.Liver: A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.Y-Box-Binding Protein 1: Y-box-binding protein 1 was originally identified as a DNA-binding protein that interacts with Y-box PROMOTER REGIONS of MHC CLASS II GENES. It is a highly conserved transcription factor that regulates expression of a wide variety of GENES.Integrin alphaV: An alpha integrin with a molecular weight of 160-kDa that is found in a variety of cell types. It undergoes posttranslational cleavage into a heavy and a light chain that are connected by disulfide bonds. Integrin alphaV can combine with several different beta subunits to form heterodimers that generally bind to RGD sequence-containing extracellular matrix proteins.Genes, Reporter: Genes whose expression is easily detectable and therefore used to study promoter activity at many positions in a target genome. In recombinant DNA technology, these genes may be attached to a promoter region of interest.Blotting, Western: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Luminescent Proteins: Proteins which are involved in the phenomenon of light emission in living systems. Included are the "enzymatic" and "non-enzymatic" types of system with or without the presence of oxygen or co-factors.Deoxycytidine Monophosphate: Deoxycytidine (dihydrogen phosphate). A deoxycytosine nucleotide containing one phosphate group esterified to the deoxyribose moiety in the 2'-,3'- or 5- positions.Luciferases: Enzymes that oxidize certain LUMINESCENT AGENTS to emit light (PHYSICAL LUMINESCENCE). The luciferases from different organisms have evolved differently so have different structures and substrates.Viral Plaque Assay: Method for measuring viral infectivity and multiplication in CULTURED CELLS. Clear lysed areas or plaques develop as the VIRAL PARTICLES are released from the infected cells during incubation. With some VIRUSES, the cells are killed by a cytopathic effect; with others, the infected cells are not killed but can be detected by their hemadsorptive ability. Sometimes the plaque cells contain VIRAL ANTIGENS which can be measured by IMMUNOFLUORESCENCE.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Fluorescent Antibody Technique: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.Complement Fixation Tests: Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.Virus Attachment: The binding of virus particles to receptors on the host cell surface. For enveloped viruses, the virion ligand is usually a surface glycoprotein as is the cellular receptor. For non-enveloped viruses, the virus CAPSID serves as the ligand.Neoplasm Transplantation: Experimental transplantation of neoplasms in laboratory animals for research purposes.Gene Deletion: A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.Retinoblastoma Protein: Product of the retinoblastoma tumor suppressor gene. It is a nuclear phosphoprotein hypothesized to normally act as an inhibitor of cell proliferation. Rb protein is absent in retinoblastoma cell lines. It also has been shown to form complexes with the adenovirus E1A protein, the SV40 T antigen, and the human papilloma virus E7 protein.Virology: The study of the structure, growth, function, genetics, and reproduction of viruses, and VIRUS DISEASES.Genes: A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.Cell Division: The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.DNA: A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).Protein Biosynthesis: The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.AIDS Vaccines: Vaccines or candidate vaccines containing inactivated HIV or some of its component antigens and designed to prevent or treat AIDS. Some vaccines containing antigens are recombinantly produced.Ganciclovir: An ACYCLOVIR analog that is a potent inhibitor of the Herpesvirus family including cytomegalovirus. Ganciclovir is used to treat complications from AIDS-associated cytomegalovirus infections.Phylogeny: The relationships of groups of organisms as reflected by their genetic makeup.Eye Infections, Viral: Infections of the eye caused by minute intracellular agents. These infections may lead to severe inflammation in various parts of the eye - conjunctiva, iris, eyelids, etc. Several viruses have been identified as the causative agents. Among these are Herpesvirus, Adenovirus, Poxvirus, and Myxovirus.Cell Survival: The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.Transcriptional Activation: Processes that stimulate the GENETIC TRANSCRIPTION of a gene or set of genes.Species Specificity: The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.Kinetics: The rate dynamics in chemical or physical systems.Cytoplasm: The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990)Receptors, Vitronectin: Receptors such as INTEGRIN ALPHAVBETA3 that bind VITRONECTIN with high affinity and play a role in cell migration. They also bind FIBRINOGEN; VON WILLEBRAND FACTOR; osteopontin; and THROMBOSPONDINS.Siadenovirus: A genus of ADENOVIRIDAE comprising species including viruses of frogs (FROGS AND TOADS) and TURKEYS. The type species is Frog adenovirus.Genetic Engineering: Directed modification of the gene complement of a living organism by such techniques as altering the DNA, substituting genetic material by means of a virus, transplanting whole nuclei, transplanting cell hybrids, etc.Gene Targeting: The integration of exogenous DNA into the genome of an organism at sites where its expression can be suitably controlled. This integration occurs as a result of homologous recombination.Chloramphenicol O-Acetyltransferase: An enzyme that catalyzes the acetylation of chloramphenicol to yield chloramphenicol 3-acetate. Since chloramphenicol 3-acetate does not bind to bacterial ribosomes and is not an inhibitor of peptidyltransferase, the enzyme is responsible for the naturally occurring chloramphenicol resistance in bacteria. The enzyme, for which variants are known, is found in both gram-negative and gram-positive bacteria. EC 2.3.1.28.Centrifugation, Density Gradient: Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Reverse Transcriptase Polymerase Chain Reaction: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.Fibroblasts: Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.Carcinoma: A malignant neoplasm made up of epithelial cells tending to infiltrate the surrounding tissues and give rise to metastases. It is a histological type of neoplasm but is often wrongly used as a synonym for "cancer." (From Dorland, 27th ed)Immunization, Secondary: Any immunization following a primary immunization and involving exposure to the same or a closely related antigen.Templates, Genetic: Macromolecular molds for the synthesis of complementary macromolecules, as in DNA REPLICATION; GENETIC TRANSCRIPTION of DNA to RNA, and GENETIC TRANSLATION of RNA into POLYPEPTIDES.E1A-Associated p300 Protein: A member of the p300-CBP transcription factors that was originally identified as a binding partner for ADENOVIRUS E1A PROTEINS.Lung: Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.Open Reading Frames: A sequence of successive nucleotide triplets that are read as CODONS specifying AMINO ACIDS and begin with an INITIATOR CODON and end with a stop codon (CODON, TERMINATOR).TritiumAntigens, Polyomavirus Transforming: Polyomavirus antigens which cause infection and cellular transformation. The large T antigen is necessary for the initiation of viral DNA synthesis, repression of transcription of the early region and is responsible in conjunction with the middle T antigen for the transformation of primary cells. Small T antigen is necessary for the completion of the productive infection cycle.CHO Cells: CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). The species is a favorite for cytogenetic studies because of its small chromosome number. The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells.Virus Inactivation: Inactivation of viruses by non-immune related techniques. They include extremes of pH, HEAT treatment, ultraviolet radiation, IONIZING RADIATION; DESICCATION; ANTISEPTICS; DISINFECTANTS; organic solvents, and DETERGENTS.Flow Cytometry: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.Disease Models, Animal: Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.Trans-Activators: Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.Cell-Free System: A fractionated cell extract that maintains a biological function. A subcellular fraction isolated by ultracentrifugation or other separation techniques must first be isolated so that a process can be studied free from all of the complex side reactions that occur in a cell. The cell-free system is therefore widely used in cell biology. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p166)Cercopithecus aethiops: A species of CERCOPITHECUS containing three subspecies: C. tantalus, C. pygerythrus, and C. sabeus. They are found in the forests and savannah of Africa. The African green monkey (C. pygerythrus) is the natural host of SIMIAN IMMUNODEFICIENCY VIRUS and is used in AIDS research.Injections, Intramuscular: Forceful administration into a muscle of liquid medication, nutrient, or other fluid through a hollow needle piercing the muscle and any tissue covering it.Restriction Mapping: Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.CD8-Positive T-Lymphocytes: A critical subpopulation of regulatory T-lymphocytes involved in MHC Class I-restricted interactions. They include both cytotoxic T-lymphocytes (T-LYMPHOCYTES, CYTOTOXIC) and CD8+ suppressor T-lymphocytes.Cytomegalovirus: A genus of the family HERPESVIRIDAE, subfamily BETAHERPESVIRINAE, infecting the salivary glands, liver, spleen, lungs, eyes, and other organs, in which they produce characteristically enlarged cells with intranuclear inclusions. Infection with Cytomegalovirus is also seen as an opportunistic infection in AIDS.Virus Assembly: The assembly of VIRAL STRUCTURAL PROTEINS and nucleic acid (VIRAL DNA or VIRAL RNA) to form a VIRUS PARTICLE.Neoplasms: New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms.Deoxyribonucleoproteins: Proteins conjugated with deoxyribonucleic acids (DNA) or specific DNA.Bacteriophage PRD1: Bacteriophage and type species in the genus Tectivirus, family TECTIVIRIDAE. They are specific for Gram-negative bacteria.Simplexvirus: A genus of the family HERPESVIRIDAE, subfamily ALPHAHERPESVIRINAE, consisting of herpes simplex-like viruses. The type species is HERPESVIRUS 1, HUMAN.Rabbits: The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.Antibodies, Neutralizing: Antibodies that reduce or abolish some biological activity of a soluble antigen or infectious agent, usually a virus.Enterovirus B, Human: A species of ENTEROVIRUS infecting humans and containing 36 serotypes. It is comprised of all the echoviruses and a few coxsackieviruses, including all of those previously named coxsackievirus B.Immunization: Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).Peptide Biosynthesis: The production of PEPTIDES or PROTEINS by the constituents of a living organism. The biosynthesis of proteins on RIBOSOMES following an RNA template is termed translation (TRANSLATION, GENETIC). There are other, non-ribosomal peptide biosynthesis (PEPTIDE BIOSYNTHESIS, NUCLEIC ACID-INDEPENDENT) mechanisms carried out by PEPTIDE SYNTHASES and PEPTIDYLTRANSFERASES. Further modifications of peptide chains yield functional peptide and protein molecules.Viral Core Proteins: Proteins found mainly in icosahedral DNA and RNA viruses. They consist of proteins directly associated with the nucleic acid inside the NUCLEOCAPSID.Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.Virus Internalization: The entering of cells by viruses following VIRUS ATTACHMENT. This is achieved by ENDOCYTOSIS, by direct MEMBRANE FUSION of the viral membrane with the CELL MEMBRANE, or by translocation of the whole virus across the cell membrane.Ebola Vaccines: Vaccines or candidate vaccines used to prevent EBOLA HEMORRHAGIC FEVER.T-Lymphocytes, Cytotoxic: Immunized T-lymphocytes which can directly destroy appropriate target cells. These cytotoxic lymphocytes may be generated in vitro in mixed lymphocyte cultures (MLC), in vivo during a graft-versus-host (GVH) reaction, or after immunization with an allograft, tumor cell or virally transformed or chemically modified target cell. The lytic phenomenon is sometimes referred to as cell-mediated lympholysis (CML). These CD8-positive cells are distinct from NATURAL KILLER CELLS and NATURAL KILLER T-CELLS. There are two effector phenotypes: TC1 and TC2.Enhancer Elements, Genetic: Cis-acting DNA sequences which can increase transcription of genes. Enhancers can usually function in either orientation and at various distances from a promoter.Temperature: The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.Nasopharynx: The top portion of the pharynx situated posterior to the nose and superior to the SOFT PALATE. The nasopharynx is the posterior extension of the nasal cavities and has a respiratory function.Genes, p53: Tumor suppressor genes located on the short arm of human chromosome 17 and coding for the phosphoprotein p53.TATA Box: A conserved A-T rich sequence which is contained in promoters for RNA polymerase II. The segment is seven base pairs long and the nucleotides most commonly found are TATAAAA.Immunohistochemistry: Histochemical localization of immunoreactive substances using labeled antibodies as reagents.

CAR-dependent and CAR-independent pathways of adenovirus vector-mediated gene transfer and expression in human fibroblasts. (1/2914)

Primary fibroblasts are not efficiently transduced by subgroup C adenovirus (Ad) vectors because they express low levels of the high-affinity Coxsackie virus and adenovirus receptor (CAR). In the present study, we have used primary human dermal fibroblasts as a model to explore strategies by which Ad vectors can be designed to enter cells deficient in CAR. Using an Ad vector expressing the human CAR cDNA (AdCAR) at high multiplicity of infection, primary fibroblasts were converted from being CAR deficient to CAR sufficient. Efficiency of subsequent gene transfer by standard Ad5-based vectors and Ad5-based vectors with alterations in penton and fiber was evaluated. Marked enhancement of binding and transgene expression by standard Ad5 vectors was achieved in CAR-sufficient fibroblasts. Expression by AdDeltaRGDbetagal, an Ad5-based vector lacking the arginine-glycine-aspartate (RGD) alphaV integrin recognition site from its penton base, was achieved in CAR-sufficient, but not CAR-deficient, cells. Fiber-altered Ad5-based vectors, including (a) AdF(pK7)betagal (bearing seven lysines on the end of fiber) (b) AdF(RGD)betagal (bearing a high-affinity RGD sequence on the end of fiber), and (c) AdF9sK betagal (bearing a short fiber and Ad9 knob), demonstrated enhanced gene transfer in CAR-deficient fibroblasts, with no further enhancement in CAR-sufficient fibroblasts. Together, these observations demonstrate that CAR deficiency on Ad targets can be circumvented either by supplying CAR or by modifying the Ad fiber to bind to other cell-surface receptors.  (+info)

Reduced phosphorylation of p50 is responsible for diminished NF-kappaB binding to the major histocompatibility complex class I enhancer in adenovirus type 12-transformed cells. (2/2914)

Reduced cell surface levels of major histocompatibility complex class I antigens enable adenovirus type 12 (Ad12)-transformed cells to escape immunosurveillance by cytotoxic T lymphocytes (CTL), contributing to their tumorigenic potential. In contrast, nontumorigenic Ad5-transformed cells harbor significant cell surface levels of class I antigens and are susceptible to CTL lysis. Ad12 E1A mediates down-regulation of class I transcription by increasing COUP-TF repressor binding and decreasing NF-kappaB activator binding to the class I enhancer. The mechanism underlying the decreased binding of nuclear NF-kappaB in Ad12-transformed cells was investigated. Electrophoretic mobility shift assay analysis of hybrid NF-kappaB dimers reconstituted from denatured and renatured p50 and p65 subunits from Ad12- and Ad5-transformed cell nuclear extracts demonstrated that p50, and not p65, is responsible for the decreased ability of NF-kappaB to bind to DNA in Ad12-transformed cells. Hypophosphorylation of p50 was found to correlate with restricted binding of NF-kappaB to DNA in Ad12-transformed cells. The importance of phosphorylation of p50 for NF-kappaB binding was further demonstrated by showing that an NF-kappaB dimer composed of p65 and alkaline phosphatase-treated p50 from Ad5-transformed cell nuclear extracts could not bind to DNA. These results suggest that phosphorylation of p50 is a key step in the nuclear regulation of NF-kappaB in adenovirus-transformed cells.  (+info)

Microtubule-dependent plus- and minus end-directed motilities are competing processes for nuclear targeting of adenovirus. (3/2914)

Adenovirus (Ad) enters target cells by receptor-mediated endocytosis, escapes to the cytosol, and then delivers its DNA genome into the nucleus. Here we analyzed the trafficking of fluorophore-tagged viruses in HeLa and TC7 cells by time-lapse microscopy. Our results show that native or taxol-stabilized microtubules (MTs) support alternating minus- and plus end-directed movements of cytosolic virus with elementary speeds up to 2.6 micrometer/s. No directed movement was observed in nocodazole-treated cells. Switching between plus- and minus end-directed elementary speeds at frequencies up to 1 Hz was observed in the periphery and near the MT organizing center (MTOC) after recovery from nocodazole treatment. MT-dependent motilities allowed virus accumulation near the MTOC at population speeds of 1-10 micrometer/min, depending on the cell type. Overexpression of p50/dynamitin, which is known to affect dynein-dependent minus end-directed vesicular transport, significantly reduced the extent and the frequency of minus end-directed migration of cytosolic virus, and increased the frequency, but not the extent of plus end-directed motility. The data imply that a single cytosolic Ad particle engages with two types of MT-dependent motor activities, the minus end- directed cytoplasmic dynein and an unknown plus end- directed activity.  (+info)

Differences in the interactions of oncogenic adenovirus 12 early region 1A and nononcogenic adenovirus 2 early region 1A with the cellular coactivators p300 and CBP. (4/2914)

Association with the cellular coactivators p300 and CBP is required for the growth-regulatory function of adenoviral (Ad) early region 1A (E1A) proteins. E1A regions necessary for these interactions overlap with domains involved in the induction of tumours in immunocompetent rodents through highly oncogenic Ad12. Differences in the association of cellular factors with the respective E1A domains of Ad12 and nononcogenic Ad2 might therefore be involved in serotype-specific oncogenicity. We analyzed the interaction of the Ad12 E1A 235R protein with p300 and CBP. Here we demonstrate that in the case of Ad12, but not Ad2/5, amino acids (aa) 1-29 of E1A proteins are sufficient to bind the p300-C/H3 domain in vivo and wild-type p300 in vitro. The conserved arginine-2, which is essential for the interaction between Ad2 E1A and p300, was dispensable for the Ad12 E1A 235R-p300 interaction in vitro. In addition to the p300-C/H3 region, we identified a second domain within p300 (aa 1999-2200) binding to the 235R protein. Contrary to p300, the amino-terminus and CR1 are necessary to associate with CBP. The aa 1-29 of the 235R protein but not CR1 are essential for the repression of colTRE-driven gene expression. This repression function is strictly dependent on p300 but not on CBP.  (+info)

Evidence for an adenovirus type 2-coded early glycoprotein. (5/2914)

We have identified an adenovirus type 2 (Ad2)-induced early glycopolypeptide with an apparent molecular weight of 20,000 to 21,000 (20/21K), as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The 20/21K polypeptide could be labeled in vivo with [(3)H]glucosamine. [(35)S]methionine- and [(3)H]-glucosamine-labeled 20/21K polypeptides bound to concanavalin A-Sepharose columns and were eluted with 0.2 M methyl-alpha-d-mannoside. The pulse-labeled polypeptide appeared as a sharp band with an apparent molecular weight of 21K, but after a chase it converted to multiple bands with an average molecular weight of 20K. This variability in electrophoretic mobility is consistent with glycosylation or deglycosylation of the 20/21K polypeptide. Analysis of the pulse and pulse-chase-labeled forms by using partial proteolysis indicated that the polypeptides were highly related chemically, but not identical. Most of the 20/21K polypeptide is localized in the cytoplasm fraction of infected cells lysed by Nonidet P-40. The 20/21K polypeptide and a 44K polypeptide, labeled with [(35)S]methionine or [(3)H]glucosamine in Ad2-infected human cells, were precipitated by a rat antiserum against an Ad2-transformed rat cell line (T2C4), but not by antisera against three other Ad2-transformed rat cell lines, or by serum from nonimmune rats. The partial proteolysis patterns of the 20/21K and the 44K polypeptides were indistinguishable, indicating that the two polypeptides are highly related, and suggesting that the 44K polypeptide might be a dimer of the 20/21K polypeptide. The 20/21K polypeptide was also induced in Ad2-early infected monkey and hamster cells. These results imply that the 20/21K polypeptide is synthesized in Ad2-infected human, monkey, and hamster cells, and in one but not all Ad2-transformed rat cells. Thus, the 20/21K polypeptide is probably viral coded rather than cell coded and viral induced.  (+info)

Development and use of a 293 cell line expressing lac repressor for the rescue of recombinant adenoviruses expressing high levels of rabies virus glycoprotein. (6/2914)

An expression cassette designed for high-level production of rabies virus glycoprotein (RG) could not be rescued into a replication-defective, adenovirus-based vector using standard procedures. To overcome this difficulty, a 293-based cell line, designated 293LAP13, was constructed that contained and expressed a derivative of the lac repressor protein. The lac operator sequence, to which the repressor binds, was incorporated into an expression cassette, containing a promoter and intron, designed for high-level production of RG. Insertion of a single operator sequence immediately downstream of the transcription start site and the use of the 293LAP13 cell line allowed recombinant viruses that could not be isolated with 293 cells to be rescued efficiently. The operator-containing virus reached higher titres in 293LAP13 than in parental 293 cells and also produced plaques more efficiently in 293LAP13 cells. Moreover, in non-complementing human and canine cell lines, adenovirus vectors with a promoter-intron expression cassette expressed RG at much higher levels than vectors lacking the intron. These observations, together with the demonstration that expression of RG by operator-containing vectors was repressed markedly in 293LAP13 cells and that this inhibition was relieved at least partly by IPTG, suggest that the 293LAP13 cell line may be useful for the rescue and propagation of many vectors in which high expression of the desired protein prevents vector rescue in 293 cells.  (+info)

The adenoviral E1A oncoproteins interfere with the growth-inhibiting effect of the cdk-inhibitor p21(CIP1/WAF1). (7/2914)

The cdk-inhibitor p21(CIP1/WAF1) inhibits the activities of cyclin-dependent kinases and proliferating cell nuclear antigen, thereby repressing cell-cycle progression and DNA replication. Transforming oncogenes, such as E1A of human adenovirus 5 (Ad5), may interfere with such growth-inhibitory proteins. In this study, we show that in various Ad5E1-transformed cells, p21(CIP1/WAF1) is expressed and that, in general, expression is not downregulated. In addition, colony-formation assays show that in Ad5E1-transformed cells highly overexpressed p21(CIP1/WAF1) can still cause growth inhibition. FACS experiments indicate, however, that a G1 arrest induced by moderate overexpression of p21(CIP1/WAF1) can be overcome by E1A. The E1A proteins may interfere with the function of p21(CIP1/WAF1) by binding. Indeed, p21(CIP1/WAF1) binds with its cyclin/cdk-binding N terminus to the transforming N-terminal and CR1 region of the E1A proteins. Together, these results lend support to the model that E1A can interfere directly with p21(CIP1/WAF1) function and thereby stimulates cell growth.  (+info)

Early region 1 transforming functions are dispensable for mammary tumorigenesis by human adenovirus type 9. (8/2914)

Some human adenoviruses are tumorigenic in rodents. Subgroup A and B human adenoviruses generally induce sarcomas in both male and female animals, and the gene products encoded within viral early region 1 (E1 region) are both necessary and sufficient for this tumorigenicity. In contrast, subgroup D human adenovirus type 9 (Ad9) induces estrogen-dependent mammary tumors in female rats and requires the E4 region-encoded ORF1 oncoprotein for its tumorigenicity. Considering the established importance of the viral E1 region for tumorigenesis by adenoviruses, we investigated whether this viral transcription unit is also necessary for Ad9 to generate mammary tumors. The nucleotide sequence of the Ad9 E1 region indicated that the gene organization and predicted E1A and E1B polypeptides of Ad9 are closely related to those of other human adenovirus E1 regions. In addition, an Ad9 E1 region plasmid demonstrated focus-forming activity in both low-passage-number and established rat embryo fibroblasts, whereas a large deletion within either the E1A or E1B gene of this plasmid diminished transforming activity. Surprisingly, we found that introducing the same transformation-inactivating E1A and E1B deletions into Ad9 results in mutant viruses that retain the ability to elicit mammary tumors in rats. These results are novel in showing that Ad9 represents a unique oncogenic adenovirus in which the E4 region, rather than the E1 region, encodes the major oncogenic determinant in the rat.  (+info)

*Adenovirus genome

"Human Adenovirus E Genome". NCBI. Retrieved 2013-01-17. "Human adenovirus E overview". NCBI. Retrieved 2013-01-17. " ... "Protein Details for Human adenovirus E". NCBI. Retrieved 2013-01-17. Russell, WC (Jan 2009). "Adenoviruses: update on structure ... The names, locations, and properties of the 38 protein-coding genes in the Human Adenovirus E genome are given in the following ... The example used for the following description is Human adenovirus E, a mastadenovirus with a 36 Kbp genome containing 38 ...

*Adenoviridae

In humans, there are 57 accepted human adenovirus types (HAdV-1 to 57) in seven species (Human adenovirus A to G): A: 12, 18, ... B Human mastadenovirus A Human mastadenovirus B Human mastadenovirus C Human mastadenovirus D Human mastadenovirus E Human ... Adenoviruses Stanford University - Adenoviruses Adenoviruses General Concepts General information on Adenovirus DNA virus ... The two currently established receptors are: CD46 for the group B human adenovirus serotypes and the coxsackievirus adenovirus ...

*Virotherapy

Zhang, Q; Yu, YA; Wang, E; Chen, N; Danner, RL; Munson, PJ; Marincola, FM; Szalay, AA (2007). "Eradication of solid human ... In 2004, researchers from University of Texas genetically programmed a type of common cold virus Adenovirus Delta-24-RGD to ... In the 1940s-1950s some of the earliest human clinical trials with oncolytic viruses were started. However, for several years ... That antigen could be from any species of virus/bactera or even human disease antigens, for example cancer antigens. The ...

*Mastadenovirus

B Human mastadenovirus A Human mastadenovirus B Human mastadenovirus C Human mastadenovirus D Human mastadenovirus E Human ... serotype 14: can cause potentially fatal adenovirus infections. Canine adenovirus 1 (CAdV-1) can lead to death in puppies, or ... Human, mammals, and vertebrates serve as natural hosts. There are currently 25 species in this genus, including the type ... Human, mammals, and vertebrates serve as the natural host. Transmission routes are fecal-oral and respiratory. "Viral Zone". ...

*Maurice Green (virologist)

They also emerged as a vehicle for human gene therapy Also in the early 1960s, Green and others showed that human adenoviruses ... His major focus of investigation was human adenoviruses. He and his colleagues worked out the basic parameters for working with ... In 1962, scientists at the National Institutes of Health discovered that certain serotypes of human adenoviruses can induce ... Doerfler, W. (2012-12-06). The Molecular Biology of Adenoviruses I: 30 Years of Adenovirus Research 1953-1983. Springer Science ...

*Infections associated with diseases

Atkinson, R L; Dhurandhar, N V; Allison, D B; Bowen, R L; Israel, B A; Albu, J B; Augustus, A S (2004). "Human adenovirus-36 is ... JC Virus and Simian Virus 40 Infection in Humans, and Association with Human Tumors". Polyomaviruses and Human Diseases. ... Atkinson, Richard L.; Lee, Insil; Shin, Hye-Jung; He, Jia (2010). "Human adenovirus-36 antibody status is associated with ... Di Luca, D; Zorzenon, M; Mirandola, P; Colle, R; Botta, GA; Cassai, E (1995). "Human herpesvirus 6 and human herpesvirus 7 in ...

*Cross-species transmission

Diseases, such as HIV and human adenoviruses have been associated with NHP interactions. In places where contact between humans ... Bats, for example, are mammals and can directly transfer rabies to humans through bite and also through aerosolization of bat ... A host shifting event is defined as a strain that was previously zoonotic and now circulates exclusively among humans. ... Wildlife zoonotic diseases of microbial origin are also the most common group of human emerging diseases, and CST between ...

*Human nutrition

Whigham, Leah D.; Israel, Barbara A.; Atkinson, Richard L. (2006). "Adipogenic potential of multiple human adenoviruses in vivo ... Human nutrition deals with the provision of essential nutrients in food that are necessary to support human life and health. ... Humans have evolved as omnivorous hunter-gatherers over the past 250,000 years. The diet of early modern humans varied ... These compounds may be found in the human body as well as in the various types of organisms that humans consume.[medical ...

*Adenain

Ding, J.Z.; McGrath, W.J.; Sweet, R.M.; Mangel, W.F. (1996). "Crystal structure of the human adenovirus proteinase with its 11 ... This cysteine endopeptidase is coded by adenoviruses. Webster, A.; Hay, R.T.; Kemp, G. (1993). "The adenovirus protease is ... Weber, J.M.; Rawlings, N.D.; Woessner, J.F. (1998). "Adenovirus protease". In Barrett, A.J. Handbook of Proteolytic Enzymes. ... This enzyme catalyses the following chemical reaction Cleaves proteins of the adenovirus and its host cell at two consensus ...

*DNA virus

Benson SD, Bamford JK, Bamford DH, Burnett RM (1999). "Viral evolution revealed by bacteriophage PRD1 and human adenovirus coat ... Human isolates Isolates from this group have also been isolated from the cerebrospinal fluid and brains of patients with ... Human isolates Viruses in this group have been isolated from other cases of encephalitis, diarrhoea and sewage. Two viruses ... The human viruses have since been renamed Primate protoparvovirus and been placed in the genus Protoparvovirus. One proposed ...

*Nikhil Dhurandhar

Atkinson RL, Dhurandhar NV, Allison DB, Bowen RL, Israel BA, Albu JB, Augustus AS (March 2005). "Human adenovirus-36 is ... about the proposed adipogenic effect of the human adenovirus AD-36 on laboratory animals and also its association with human ... "Human Adenovirus Ad-36 Promotes Weight Gain in Male Rhesus and Marmoset Monkeys". The Journal of Nutrition. 132 (10): 3155-3160 ...

*Transfection

Graham FL, van der Eb AJ (April 1973). "A new technique for the assay of infectivity of human adenovirus 5 DNA". Virology. 52 ( ... Adenoviral vectors can be useful for viral transfection methods because they can transfer genes into a wide variety of human ... Bacchetti S, Graham FL (April 1977). "Transfer of the gene for thymidine kinase to thymidine kinase-deficient human cells by ... Viruses used to date include retrovirus, lentivirus, adenovirus, adeno-associated virus and herpes simplex virus. However, ...

*SV40

Yamamoto, Hiroshi; Shimojo, H (August 1971). "Multiplicity reactivation of human adenovirus type 12 and simian virus 40 ... "Simian virus 40 infection in humans and association with human diseases: results and hypotheses". Virology. 318 (1): 1-9. doi: ... Whether or not this is true for other human cells is debated. SV40 is believed to suppress the transcriptional properties of ... Poulin, D. L.; Decaprio, J. A. (2006). "Is There a Role for SV40 in Human Cancer?". Journal of Clinical Oncology. 24 (26): 4356 ...

*Adenovirus serotype 36

Human adenovirus 36 (HAdV-36) or Ad-36 or Adv36 is one of 52 types of adenoviruses known to infect humans. AD-36, first ... To date, AD-36 is the only human adenovirus that has been linked with human obesity, present in 30% of obese humans and 11% of ... 2007). "Human adenovirus Ad-36 induces adipogenesis via its E4 orf-1 gene". Int J Obes (Lond). 32 (3): 397-406. doi:10.1038/sj. ... Augustus A.S., Atkinson R.L.; Dhurandhar N.V.; Allison D.B.; Bowen R.L.; Israel B.A.; Albu J.B. (2005). "Human adenovirus-36 is ...

*CAAT box

... of subgroup C human adenoviruses, in species with a deficient CAAT sequence. The transcription initiation at mutant MLP species ... "Functional Analysis of the CAAT Box in the Major Late Promoter of the Subgroup C Human Adenoviruses". Journal of Virology. 72 ( ... In another experiment performed with the major late promoter (MLP) of adenoviruses from a variety of host species, it was shown ... The failure to restore the normally functional adenoviruses, exhibited by a CAAT box, is consistent with the idea that the CAAT ...

*Baby hamster kidney cell

BHK-21 cells are susceptible to human adenovirus D, reovirus 3, and vesicular stomatitis virus (Indiana strain). BHK-21 cells ...

*Jacqui Horswell

Mobility and survival of Salmonella Typhimurium and human adenovirus from spiked sewage sludge applied to soil columns (2010). ...

*E2F

pRb is one of the targets of the oncogenic human papilloma virus protein E7, and human adenovirus protein E1A. By binding to ... Tommasi S, Pfeifer GP (1995). "In vivo structure of the human cdc2 promoter: release of a p130-E2F-4 complex from sequences ...

*HEK 293 cells

Shaw G, Morse S, Ararat M, Graham FL (June 2002). "Preferential transformation of human neuronal cells by human adenoviruses ... "Characteristics of a human cell line transformed by DNA from human adenovirus type 5". J. Gen. Virol. 36 (1): 59-74. doi: ... Adenoviruses transform neuronal lineage cells much more efficiently than typical human kidney epithelial cells. An embryonic ... Louis N, Evelegh C, Graham FL (July 1997). "Cloning and sequencing of the cellular-viral junctions from the human adenovirus ...

*PRSS8

Wang C, Chao J, Chao L (Apr 2003). "Adenovirus-mediated human prostasin gene delivery is linked to increased aldosterone ... Prostasin is a protein that in humans is encoded by the PRSS8 gene. This gene encodes a trypsinogen, which is a member of the ... Yu JX, Chao L, Chao J (Jul 1994). "Prostasin is a novel human serine proteinase from seminal fluid. Purification, tissue ... Yu JX, Chao L, Chao J (Jun 1995). "Molecular cloning, tissue-specific expression, and cellular localization of human prostasin ...

*Coxsackie virus and adenovirus receptor

"Genomic organization and chromosomal localization of the human Coxsackievirus B-adenovirus receptor gene". Human Genetics. 105 ... Coxsackievirus and adenovirus receptor (CAR) is a protein that in humans is encoded by the CXADR gene. The protein encoded by ... Tomko RP, Xu R, Philipson L (Apr 1997). "HCAR and MCAR: the human and mouse cellular receptors for subgroup C adenoviruses and ... Tomko RP, Xu R, Philipson L (Apr 1997). "HCAR and MCAR: the human and mouse cellular receptors for subgroup C adenoviruses and ...

*Adenovirus infection

"Human Adenovirus Ad-36 Promotes Weight Gain in Male Rhesus and Marmoset Monkeys". J. Nutr. 132 (10): 3155-3160. PMID 12368411. ... Safe and effective adenovirus vaccines were developed for adenovirus serotypes 4 and 7, but were available only for preventing ... Enteric adenoviruses 40 and 41 cause gastroenteritis, usually in children. For some adenovirus serotypes, the clinical spectrum ... and/or association with human obesity. To date, the most thorough investigations have been conducted for adenovirus serotype 36 ...

*René Bernards

Transformation and oncogenicity by human adenoviruses. He then moved to the United States and was a post doc under Robert ...

*Cidofovir

Human herpesviruses Adenoviruses Human poxviruses (including the smallpox virus) Human papillomavirus Cidofovir was discovered ... It also inhibits human polymerases but this action is 8-600 times weaker than its actions on viral DNA polymerases. It also ... De Gascun, C. F.; Carr, M. J. (2013). "Human polyomavirus reactivation: Disease pathogenesis and treatment approaches". ...

*VA RNA

Kidd, AH; Garwicz D; Oberg M (1995). "Human and simian adenoviruses: phylogenetic inferences from analysis of VA RNA genes". ... These two VA RNA genes are distinct genes in the adenovirus genome. VA RNAI is the major species with VA RNAII expressed at a ... The VA (viral associated) RNA is a type of non-coding RNA found in adenovirus. It plays a role in regulating translation. There ... Ma, Y; Mathews MB (1996). "Secondary and tertiary structure in the central domain of adenovirus type 2 VA RNA I". RNA. 2 (9): ...

*Robert Huebner

In contrast to medical wisdom in the 1960s and 1970s, Huebner was confident that viruses were a cause of cancer in humans and ... From that culture they isolated cytomegalovirus, as well as the first of a large family of adenoviruses. Dr. Robert M. Chanock ...

*Cell culture

Novel ideas in the field include recombinant DNA-based vaccines, such as one made using human adenovirus (a common cold virus) ... Nguyen HT, Geens M, Spits C (2012). "Genetic and epigenetic instability in human pluripotent stem cells". Human Reproduction ... Culture of human stem cells is used to expand the number of cells and differentiate the cells into various somatic cell types ... In addition, chemically defined media can be used to eliminate any serum trace (human or animal), but this cannot always be ...
Transarterial chemoembolization (TACE) is currently one of the mainstays of palliative treatments worldwide for patients with unresectable Hepatocellular Carcinoma(HCC).However, the long term outcomes were generally poor for HCC patients treated with TACE. Recombinant Human Adenovirus Type 5, an E1B gene deleted adenovirus, is known to have a significant antitumor activity. In addition, local injection of recombinant human adenovirus type 5 can enhance the effect of antitumor therapies (chemotherapy and radiotherapy). The hypothesis is that patients with unresectable HCC may benefit from recombinant human adenovirus type 5 in combination with TACE ...
Summary Evidence is presented here which indicates that the adenovirus DNA-binding protein (DBP) is phosphorylated at a tyrosine residue early in infection. This was suggested by the discovery that a proportion of the label in 32P-labelled DBP was resistant to alkali, and was substantiated by acid hydrolysis of DBP immunoprecipitates and by immunoblotting with a monoclonal antibody against phosphotyrosine. Treatment of [35S] methionine-labelled DBPs with chymotrypsin produced fragments of apparent M r 45K and 39K whereas digestion of 32P-labelled DBP resulted in fragments of 45K and 26K. Consideration of the distribution of 32P label and its alkali stability in these fragments suggested that chymotrypsin cleaved populations of DBP at different sites depending on their phosphorylation states. The conservation, in all of the seven adenovirus serotypes sequenced, of a tyrosine residue (at amino acid 195 in adenovirus type 2) together with its surrounding residues, suggests that phosphorylation
Human adenoviruses are non-enveloped dsDNA viruses of almost 35 kb in size [1]. HAdV can infect a variety of tissues and cause a wide range of complications like gastroenteritis, hepatitis, myocarditis, keratoconjunctivitis and pneumonia [2, 3]. It is contagion in nature which occurs through direct contact or fomites and virus is also resistant to various physical and chemical agents. Children younger than the age of 5 years and immune compromised persons especially the pediatric patients are most susceptible to these viruses. Worldwide 5-7% respiratory tract infections are ascribed by HAdV in pediatric patients [4] and persons of all ages are susceptible to infections caused by these viruses [5].. Seven known Human adenoviruses species from HAdV-A to HAdV-G are constitute of the genus Mastadenovirus in which all the human adenoviruses are categorized and further divided into different strains [6]. Now 67 types of HAdV have been reported [7]. Their number is rapidly increasing due to ...
In 2005, a human adenovirus strain (formerly known as HAdV-D22/H8 but renamed here HAdV-D53) was isolated from an outbreak of epidemic keratoconjunctititis (EKC), a disease that is usually caused by HAdV-D8, -D19, or -D37, not HAdV-D22. To date, a complete change of tropism compared to the prototype has never been observed, although apparent recombinant strains of other viruses from species Human adenovirus D (HAdV-D) have been described. The complete genome of HAdV-D53 was sequenced to elucidate recombination events that lead to the emergence of a viable and highly virulent virus with a modified tropism. Bioinformatic and phylogenetic analyses of this genome demonstrate that this adenovirus is a recombinant of HAdV-D8 (including the fiber gene encoding the primary cellular receptor binding site), HAdV-D22, (the ε determinant of the hexon gene), HAdV-D37 (including the penton base gene encoding the secondary cellular receptor binding site), and at least one unknown or unsequenced HAdV-D strain.
In 2005, a human adenovirus strain (formerly known as HAdV-D22/H8 but renamed here HAdV-D53) was isolated from an outbreak of epidemic keratoconjunctititis (EKC), a disease that is usually caused by HAdV-D8, -D19, or -D37, not HAdV-D22. To date, a complete change of tropism compared to the prototype has never been observed, although apparent recombinant strains of other viruses from species Human adenovirus D (HAdV-D) have been described. The complete genome of HAdV-D53 was sequenced to elucidate recombination events that lead to the emergence of a viable and highly virulent virus with a modified tropism. Bioinformatic and phylogenetic analyses of this genome demonstrate that this adenovirus is a recombinant of HAdV-D8 (including the fiber gene encoding the primary cellular receptor binding site), HAdV-D22, (the ε determinant of the hexon gene), HAdV-D37 (including the penton base gene encoding the secondary cellular receptor binding site), and at least one unknown or unsequenced HAdV-D strain.
The purpose of this multi-center clinical trial is to verify more effective on local control of malignant pleural effusions in NSCLC patients by thoracic
Adenovirus genomes are linear, non-segmented double-stranded (ds) DNA molecules that are typically 26-46 Kbp long, containing 23-46 protein-coding genes. The example used for the following description is Human adenovirus E, a mastadenovirus with a 36 Kbp genome containing 38 protein-coding genes. While the precise number and identity of genes varies among adenoviruses, the basic principles of genome organization and the functions of most of the genes described in this article are shared among all adenoviruses. The 38 genes in the Human Adenovirus E genome are organized in 17 transcription units, each containing 1-8 coding sequences. Alternative splicing during processing of the pre-mRNAs produced by each transcription unit enable multiple different mRNAs to be produced from one transcription unit. The E1A, E1B, E2A, E2B, E3, and E4 transcription units are successively transcribed early in the viral reproductive cycle. The proteins coded for by genes within these transcription units are mostly ...
To define the bottlenecks that restrict antigen expression after oral administration of viral-vectored vaccines, we tracked vectors derived from the human adenovirus type 5 at whole body, tissue and cellular scales throughout the digestive tract in a murine model of oral delivery. After intragastric administration of vectors encoding firefly luciferase or a model antigen, detectable levels of transgene-encoded protein or mRNA were confined to the intestine, and restricted to delimited anatomical zones. Expression of luciferase in the form of multiple small bioluminescent foci in the distal ileum, cecum and proximal colon suggested multiple crossing points. Many foci were unassociated with visible Peyers patches, implying that transduced cells lay in proximity to villous rather than follicle-associated epithelium, as supported by detection of transgene-encoded antigen in villous epithelial cells. Transgene-encoded mRNA but not protein was readily detected in Peyers patches, suggesting that post
Human adenovirus (HAdV) is a common pathogen causing a broad spectrum of diseases. HAdV encodes the pVII protein, which is involved in nuclear delivery, protection and expression of viral DNA. To suppress the cellular interferon (IFN) and RNA interference (RNAi) systems, HAdVs encode non-coding virus-associated (VA) RNAs. In this thesis we have investigated the functional significance of the pVII protein and VA RNAI in HAdV-5 infected cells.. We report that the propeptide module is the destabilizing element targeting the precursor pVII protein for proteasomal degradation. We also found that the Cul3-based E3 ubiquitin ligase complex alter the precursor pVII protein stability via binding to the propeptide sequence. In addition, we show that inhibition of the Cul3 protein reduces HAdV-5 E1A gene expression. Collectively, our results suggest a novel function for the pVII propeptide module and involvement of Cul3 in viral E1A gene expression.. Our studies show that the cellular E3 ubiquitin ligase ...
The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details ...
Human adenovirus 12 ATCC ® VR-863D™ Designation: DNA from Human adenovirus 12 strain Huie [ATCC ® VR-863™] Application: It is suitable for use in polymerase chain reaction (PCR) for viral gene products and other molecular virology applications. Respiratory research
Human adenovirus 2 ATCC ® VR-846D™ Designation: DNA from Human adenovirus 2 strain Adenoid 6 [ATCC ® VR-846™] Application: It is suitable for use in polymerase chain reaction (PCR) for viral gene products and other molecular virology applications. Respiratory research
It has been known for some time that the human adenovirus serotype 5 (Ad5) E4orf6 and Sox18 E1B55K proteins work in concert to degrade p53 and to regulate selective export of late viral mRNAs during productive infection. H1299 human lung carcinoma cells after expression of E1B55K and E4orf6 using adenovirus vectors. Several species were detected and identified by mass spectroscopy and for one of these integrin α3 we went on in a parallel study to confirm it as a substrate of the complex (F. Dallaire et al. J. Virol. 83:5329-5338 2009 Although the system has some limitations it may still be of some general use in identifying candidate substrates of any viral cullin-based E3 ubiquitin ligase complex and we suggest a series of criteria for substrate validation. During the past decade protein degradation has become increasingly recognized SVT-40776 (Tarafenacin) as a critical mechanism by which cells regulate a number of fundamental processes (reviewed in references 37 57 and 59). Degradation ...
We report a novel human adenovirus D (HAdV-65) isolated from feces of 4 children in Bangladesh who had acute gastroenteritis. Corresponding genes of HAdV-65 were related to a hexon gene of HAdV-10, penton base genes of HAdV-37 and HAdV-58, and a fiber gene of HAdV-9. This novel virus may be a serious threat to public health.
Recombinant Human adenovirus serotype 5 (HAdV-5) is the most commonly used gene transfer vehicle with a long safety record [1]. The HAdV-5 36 kb genome (double stranded DNA) is well characterized, and used in many experimental and clinical settings [2]. Its systemic use in vaccination and gene therapy has been limited by pre-existing immunity and the presence of neutralizing antibodies in environmentally exposed individuals. Neutralizing antibodies, directed mainly against capsid hexon loops, are serotype specific. Approximately 45% of adults in the United States possess neutralizing antibodies to HAdV-5. A variety of other Adenoviral subtypes and deletion mutants have been investigated (Ad28, Ad35 and "gutless" Adenovirus), in an attempt to circumvent pre-existing immunity in exposed individuals.. We have utilized E1/E3-deleted, replication deficient tumor antigen-encoding HAdV-5 in vitro and in vivo, to promote tumor antigen-specific immunity (reviewed in [3]. As previously shown, dendritic ...
Adenoviral nucleic acid was detected by polymerase chain reaction (PCR) in pharyngeal and rectal swab samples of a cat seropositive for adenovirus and suffering from transient hepatic failure. The samples were taken at a one-year interval, and both faecal samples as well as the second pharyngeal sample were positive in ... read more PCR performed with general adenovirus primers. The size of the amplified products corresponded to that of the positive control. The identity of the amplicons was also confirmed by DNA sequencing. The 301 bp long hexon gene fragment was very similar to but distinguishable from the corresponding hexon sequence of human adenovirus type 2. This result suggests the possibility of persistent carrier status and shedding of adenovirus in cats. show less ...
The precursor of the 55K adenovirus terminal protein is an 87K protein that is covalently linked to viral DNA. This protein is likely to be identical to the 80,000 dalton protein described by Challberg et al. (1980). The mRNA for the 87K terminal protein precursor, like that for the E2-72K DNA binding protein, is detectable at both early and late times of infection, and its production is sensitive to protein synthesis inhibition (Lewis and Mathews, 1980). The 87K protein, together with proteins of 105,000 and 75,000 daltons, are translated from leftward transcribed (1-strand) messenger RNAs that are complementary to the viral genome between positions 11.2 and 31.5. Additional hybridization to the region between coordinates 37.3 and 41 suggests that the RNA body is spliced to sequences mapping farther right in the genome. Electron microscopic heteroduplex analysis has revealed a family of 1-strand RNAs that probably encode these proteins. The RNA bodies extend from coordinated 30, 26 and 23 to ...
Recombinant adenoviruses currently are used for a variety of purposes, including gene transfer in vitro, vaccination in vivo, and gene therapy (1-4). Several features of adenovirus biology have made such viruses the vectors of choice for certain of these applications. For example, adenoviruses transfer genes to a broad spectrum of cell types, and gene transfer is not dependent on active cell division. Additionally, high titers of viruses and high levels of transgene expression generally can be obtained.. Decades of study of adenovirus biology have resulted in a detailed picture of the viral life cycle and the functions of the majority of viral proteins (5, 6). The genome of the most commonly used human adenovirus (serotype 5) consists of a linear, 36-kb, double-stranded DNA molecule. Both strands are transcribed and nearly all transcripts are heavily spliced. Viral transcription units are conventionally referred to as early (E1, E2, E3, and E4) and late, depending on their temporal expression ...
Subspecies B1 human adenoviruses (HAdVs) are important causes of acute respiratory disease in pediatric and military recruit populations. Although extensive epidemiological data document the genetic diversity of these human pathogens, little is known about the relevance of this genetic diversity on the pathogenesis and fitness of subspecies B1 HAdVs. Additionally, the unique molecular biology of these pathogens is understudied compared to the species C HAdV serotypes 2 and 5. One of the uniquely diverse regions of the HAdV genomes is the early region 3 (E3) transcription unit. These genes are implicated in pathogenesis, host-species specificity, and the modulation of the host immune response to infection. Subspecies B1 HAdVs encode a set of novel open reading frames (ORFs) within the E3 region, including E3-20.1K, E3-20.5K, and E3-10.9K. ORF E3-10.9K is highly polymorphic among subspecies B1 HAdVs, and it displays extensive variation among strains of serotypes 3 and 7. In an effort to
The cryo-electron microscopy (cryo-EM) structure of the complex between the trimeric human adenovirus B serotype 3 fibre knob and human desmoglein 2 fragments containing cadherin domains EC2 and EC3 has been published, showing 3:1 and 3:2 complexes. Here, the crystal structure determined at 4.5 Å resolution is presented with one EC2-EC3 desmoglein fragment bound per fibre knob monomer in...
Adenovirus has been associated with both sporadic and epidemic disease and, with regard to infections among military recruits, who were routinely immunized against types 4 and 7 from 1971 until the cessation of vaccine production in 1996. Adenovirus became a significant cause of economic cost and morbidity in this setting. A live oral vaccine against adenovirus types 4 and 7 was approved for use in this population by the US Food and Drug Administration (FDA) in 2011, and subsequent incidence of acute respiratory disease declined.. Of interest is the role of adenoviruses as vectors in vaccination and in gene therapy. [1, 2, 3] Adenoviruses can infect various cells, both proliferating and quiescent, and thus hold the promise of targeting many different tissues and diseased cell lines.. The genome of adenovirus is well known and can be modified with relative ease to induce lysis or cytotoxicity of a specified cell line without affecting others.. The virus itself can be engineered to remove its ...
The E4 region of human adenovirus type 2 is predicted to encode seven proteins as judged from its nucleotide sequence and the pattern of differential splicing of its transcript. Two of the open reading frames (ORFs), ORF1 and ORF2, had been identified as being disrupted in the recently published sequence of the related serotype 5 virus. These ORFs were resequenced and found to be intact in the wt300 strain of adenovirus type 5.
Most adenoviruses bind directly to the coxsackie and adenovirus receptor (CAR) on target cells in vitro, but recent research has shown that adenoviruses can also use soluble components in body fluids for indirect binding to target cells. These mechanisms have been identified upon addressing the questions of how to de- and retarget adenovirus-based vectors for human gene and cancer therapy, but the newly identified mechanisms also suggest that the role of body fluids and their components may also be of importance for natural, primary infections. Here we demonstrate that plasma, saliva, and tear fluid promote binding and infection of adenovirus type 5 (Ad5) in respiratory and ocular epithelial cells, which corresponds to the natural tropism of most adenoviruses, and that plasma promotes infection by Ad31. By using a set of binding and infection experiments, we also found that Ad5 and Ad31 require coagulation factors IX (FIX) or X (FX) or just FIX, respectively, for efficient binding and infection. ...
Your basket is currently empty. i ,p>When browsing through different UniProt proteins, you can use the basket to save them, so that you can back to find or analyse them later.,p>,a href=/help/basket target=_top>More...,/a>,/p> ...
Intrinsic disorder in the common N-terminus of human adenovirus 5 E1B-55K and its related E1BN proteins indicated by studies on E1B- ...
Full custom adenovirus production services, adenovirus vector construction, adenovirus packaging, adenovirus amplification, adenovirus purification, adenovirus titering.
Find more information on adenovirus vector including adenovirus infection and adenovirus symptoms in Kidspots comprehensive health section.
SignaGen Laboratories, A Gene Delivery Company Providing Custom AAV Adenovirus Lentivirus Production Services & Manufacturing DNA/siRNA Transfection Reagents... Ad-PRKD2 [SL100949] - Product Category: Human Adenovirus Type5 (dE1/E3) expressing Protein Kinase D2 (PRKD2) under CMV promoter. Product Information Product Name: Protein Kinase D2, cloned into our Ad-MAX adenovirus expression system, ready to package. Promoter: CMV Titer: 1E+10~1E+11 PFU/ml Storage Buffer: DMEM with 2.5% BSA, 2.5% glycerol Gene Information Gene Name: Protein Kinase D2 NCBI Acc. #: NM_016457 Gene Symbol: PRKD2 Gene ID:
SignaGen Laboratories, A Gene Delivery Company Providing Custom AAV Adenovirus Lentivirus Production Services & Manufacturing DNA/siRNA Transfection Reagents... Ad-HIPK2 [SL101018] - Product Category: Human Adenovirus Type5 (dE1/E3) expressing Homeodomain Interacting Protein Kinase 2 under CMV promoter. Product Information Product Name: Homeodomain Interacting Protein Kinase 2, pre-made adenovirus, ready to ship and ready to use format. Promoter: CMV Titer: 1E+11 ~ 1E+12 PFU/ml Storage Buffer: DMEM with 2.5% BSA, 2.5% glycerol Gene Information Gene Name: Homeodomain Interacting Protein Kinase
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
There are many stages in the development of a new drug for viral infection and such processes are even further complicated for adenovirus by the fact that there are at least 51 serotypes, forming six distinct groups (A-F), with different degree of infectivity. This review attempts to address the importance of developing pharmaceuticals for adenovirus and also review recent development in drug discovery for adenovirus, including newer strategies such as microRNA approaches. Different drug screening strategies will also be discussed.
Adenovirus Type 9, 0.1 mg. The many different serotypes of human adenoviruses (Ad) are divided into six subgroups, of which all Ad subgroup A and B and two subgroup D Ads can elicit tumors in infected rodents.
A HIV-1 DNA prime-recombinant Adenovirus Type 5 (rAd5) increase vaccine failed to guard against HIV-1 acquisition. is certainly towards the gp41 subunit from the envelope (Env) glycoprotein from the pathogen (1). This antibody response derives from polyreactive B cells that cross-react with Env and intestinal microbiota (IM) (2, 3). Nevertheless, it is unidentified if an identical gp41-reactive Ab response would take place in the placing of HIV-1 Env vaccination. A DNA leading, recombinant adenovirus serotype 5 (rAd5) increase vaccine that included HIV and genes, and a trivalent combination of clade A, B and C gp140 genes formulated with both gp120 and gp41 elements was examined in the HIV Vaccine Studies Network (HVTN) [stage Ib (HVTN 082), GW-786034 stage II (HVTN 204), stage IIb (HVTN 505) efficiency trial] and various other clinical studies [stage I/II (RV172), stage I (V001)] (4C7). This vaccine was the GW-786034 initial vaccine formulated with the ectodomain from the Env gp41 component, ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
In tumor cells, the p53 pathway is often disrupted. Therefore, recovering the function of wild-type p53 and its targets in tumor cells is a key therapeutic objective. In head and neck cancer, p53 mutations are frequent, and the incidence of p53 mutations increases with progression of head and neck cancer (46, 47). Therefore, a recombinant human adenovirus that expresses functional wild-type p53 has been approved by the Chinese government for the treatment of head and neck carcinoma (48-50). Treatments showed that antitumor efficacy was associated with the expression and activity of functional p53, and adverse effects were also significant (51-54). Recently, pharmacologically activated wild-type p53 by small-molecule compound RITA is reported to inhibit glycolytic enzymes and, therefore, induce robust apoptosis in cancer cells (55). In addition, enhancement of p53 protein stability is also a target in restoring wild-type p53 activity in cancer cells. The protein level of wild-type p53 is ...
Mouse anti Adenovirus Hexon antibody, clone 7C11 reacts with human, canine, bovine, monkey and rat adenoviruses. It is very likely that it
13:2; potential epub Adenovirus notions; tenure bhakti 1? On the epub Adenovirus Methods and of this interest, are above under form. 1282 An epub Adenovirus Methods and Protocols: Adenoviruses, Ad study read by Porten - Szubin 1987:187.
Looking for Dog adenovirus? Find out information about Dog adenovirus. acute viral disease of canines, especially dogs and foxes. The causative agent, an adenovirus, is not infectious to humans. In foxes the disease is... Explanation of Dog adenovirus
1NLN: Crystallographic structure at 1.6-A resolution of the human adenovirus proteinase in a covalent complex with its 11-amino-acid peptide cofactor: insights on a new fold
Much concern has focused on the direct toxic effects of adenoviruses, particularly as intravenous administration of the virus can induce acute liver injury, as shown in animal models. It is this effect which may have triggered the cascade of events leading to the death of the patient with OTC deficiency-in this case the recombinant virus was injected directly into the hepatic artery. Studies in mice have highlighted the dose limiting liver toxicity of intravenously administered virus, which in this model is mainly due to an acute inflammatory response involving the release of certain cytokines (interleukin 6, interleukin 8, tumour necrosis factor α) and the recruitment of immune effector cells into the liver.5-7These effects are manifest within the first few hours of adenovirus administration and do not require de novo virus gene expression. A recent study demonstrated that adenovirus induced chemokine gene expression within the liver occurs within one hour after infection and results in the ...
Premade Human MIR1538 Adenovirus (NR_031719), ready-to-use and available with your choice of HA tag, His tag, GFP reporter or untagged.
Gentaur molecular products has all kinds of products like :search , bio-gentaur \ VIROLOGY Adenovirus Type 5 Culture Fluid \ GEN0810020CF for more molecular products just contact us
Buy L3 antigen, ADENOVIRUS TYPE 5 Antigen-AP_000211.1 (MBS239153) product datasheet at MyBioSource, Antigens. Application: ELISA (EIA)
View Adenovirus Type 3 Lysate (1 mg) 0810062 from our online collection of viruses, microorganisms, and other products for infectious disease diagnostic development. Browse our larger selection of Microorganisms,Microorganisms,Microorganisms for Assay Developers,Research and Development,Clinical Laboratories products from ZeptoMetrix.
Gentaur molecular products has all kinds of products like :search , bio-gentaur \ Adenovirus Type 7A Sucrose Purified \ GEN0810021SP for more molecular products just contact us
What is the difference between Adenovirus and Retrovirus? Adenovirus is a virus type without an envelope whereas retroviruses are virus type with an envelope...
The early region (E1) of the adenovirus genome, responsible for transforming activity, is localized within the left most 11% of the viral genome and…
The regions of the human adenoviral genome associated with the process of oncogenesis have been identified using several approaches. Analysis of the viral DNAs contained in different lines of cells transformed by virus has demonstrated that retention of the leftmost 14% of the genome is sufficient for the maintenance of the transformed growth properties of these cells (Gallimore et al. 1974). The adenoviral mRNAs expressed in transformed cell lines are similar to those expressed from these DNA sequences during the early phase of the productive infection (Flint et al. 1975). The left end of the viral DNA contains at least two genes necessary for transformation, since two complementation groups of host-range mutants that map within this region (Frost and Williams 1978) are both defective for transformation (Graham et al. 1978). Transfection of cells with fragments of viral DNA has provided a direct means of determining the minimum amount of viral... ...
Adenoviruses are ubiquitous. Weve all been infected with lots of them (gets to be a problem when making gene therapy vectors). The older you are, the more likely you have been exposed to the different kinds of adenovirus, to the point where if you are an adult reading this, you probably have antibodies to serotypes 1, 2, 5, and/or 6 (depending on where you live).. Thus older children are more likely to have been exposed to any adenovirus, including Ad-36. If your obese group of children is 63% kids aged 15-18, and your healthy weight group is only 17% 15-18, I am not shocked at all that antibodies to Ad-36 is correlated with obesity. I bet you could have picked any other adenovirus serotype and found the same correlation. But they didnt look.. William M. Briggs gets it. I dunno the dude. Statistician. But he gets it. *thumbs-up*. From a virologists perspective, there are putative, plausible mechanisms for how Ad-36 could actually play a role in obesity. Those reasonings require ongoing ...
Molecular model of the fibre knob protein from an Adenovirus complexed with its human cellular receptor, the coxsackie and adenovirus receptor (CAR). The binding of the fibre knob protein to CAR allows the virus entry to the cell. - Stock Image C025/1656
眼窩静脈叢採取血液と心臓採取血液の血液生化学値の比較-眼窩静脈叢採取血液での酵素値の上昇- / p99 (0165.jp2 ...
Strunze, S; Engelke, M F; Wang, I-H; Puntener, D; Boucke, K; Schleich, S; Way, M; Schoenenberger, P; Burckhardt, C J; Greber, U F (2011). Kinesin-1-mediated capsid disassembly and disruption of the nuclear pore complex promote virus infection. Cell Host & Microbe, 10(3):210-223.. Püntener, D; Engelke, M F; Ruzsics, Z; Strunze, S; Wilhelm, C; Greber, U F (2011). Stepwise loss of fluorescent core protein V from human adenovirus during entry into cells. Journal of Virology, 85(1):481-496.. Strunze, S; Trotman, L C; Boucke, K; Greber, U F (2005). Nuclear targeting of adenovirus type 2 requires CRM1-mediated nuclear export. Molecular Biology of the Cell, 16(6):2999-3009.. ...
[205 Pages Report] Check for Discount on Adenovirus Diagnostic Testing Market: US, Europe, Japan report by Venture Planning Group. The report presents a detailed analysis of the Adenovirus diagnostics...
Adenovirus, a DNA virus, was first isolated in the 1950s in adenoid tissue-derived cell cultures, hence the name. These primary cell cultures were often noted to spontaneously degenerate over time, and adenoviruses are now known to be a common cause of asymptomatic respiratory tract infection that produces in vitro cytolysis in these tissues.
Export of adenoviral late mRNA from the nucleus requires the Nxf1/Tap export receptor.: One important function of the human adenovirus E1B 55-kDa protein is ind
Detect adenovirus rescue even before cytopathic effect (CPE) is observed. Ensure high titer by harvesting amplified adenovirus at the optimal time
Mouse Monoclonal Anti-Adenovirus Hexon Antibody (3G0) [DyLight 488]. Validated: ELISA, ICC/IF. Tested Reactivity: Virus. 100% Guaranteed.
4EKF: Regulation of a Viral Proteinase by a Peptide and DNA in One-dimensional Space: III. ATOMIC RESOLUTION STRUCTURE OF THE NASCENT FORM OF THE ADENOVIRUS PROTEINASE.
Adenovirus can define as the virus which belongs to the group-1, ds-DNA human virus which can most usually cause acute respiratory infections and occasionally cause conjunctivitis, cystitis and gastroenteritis.
Molecular Cloning, also known as Maniatis, has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential.
This post is about adenovirus infection, a major cause of illness both minor and severe in the United States, especially among children in group settings.
Our study is the first report of an RSAd, in which the "promoter-based regulation of E1A," approach is used to target the deregulated G1 to S phase in tumor cells. We demonstrated that AdE2F-1RC replicated selectively in tumor cells and not in normal cells expressing high and low levels of E2F-1 protein, respectively. Additionally, in two mouse xenograft models, AdE2F-1RC exhibited significant in vivo therapeutic benefit often equivalent to wild-type adenovirus treatment. These studies validate several design features of AdE2F-1RC.. The wild-type adenovirus dl309 replicated in all of the normal cells tested. We reasoned that normal resting cells would be a good model for AdE2F-1RC toxicity tests, because these cells do not express E2F-1 (44 , 45) and are found in the tumor environment. In contrast to dl309, the replication and CPE of AdE2F-1RC was significantly attenuated in normal cells suggesting that the E2F-1 promoter was not optimally activated. One reason is that the presence of pRb/E2F-1 ...
Adenovirus 36 (AdV36) causes increased adiposity (leading to obesity) and metabolic changes in animals. Causation in humans has not been established, but the in...
Premade Fish RFP (Red Fluorescent Protein) Adenovirus (Q9U6Y8), ready-to-use and available with your choice of HA tag, His tag, GFP reporter or untagged.
Learn more about Infección por Adenovirus at Blake Medical Center DefiniciónCausasFactores de riesgoSíntomasDiagnósticoTratamientoPrevenció...
ONCOS-102 (previously known as CGTG-102) is a genetically modified replication competent oncolytic human adenovirus based on serotype 5. It is armed with GMCSF transgene and has a 24 bp deletion restraining the replication exclusively in tumors. The viral capsid has been modified for effective transduction of tumor cells. ...
Our results show that the elimination of p53 is not required for efficient replication and release of adenovirus type 5. The observations were made in primary cells or in cell lines that are not compromised for the expression of p14ARF. These results challenge the concept of using adenoviruses deficient in antagonizing p53 to treat cancer patients, raising the need to develop alternative strategies to construct tumor-selective viruses.. Previously (28) , it has been reported that the replication of the virus dl1520/ONYX 015 lacking E1B-55 kDa is suppressed in cells containing wild-type p53, at least when p14ARF is expressed in the same cells (52) . The proposed correlation between p53 status and replication of this virus was not supported in many subsequent reports (34 , 42, 43, 44, 45, 46, 47, 48) . Some of the cells that replicate the virus efficiently despite the presence of wild-type p53 may indeed lack functional p14ARF, thereby leaving open the possibility that p53 might act as an ...
This patent search tool allows you not only to search the PCT database of about 2 million International Applications but also the worldwide patent collections. This search facility features: flexible search syntax; automatic word stemming and relevance ranking; as well as graphical results.
Recognizes and binds the palindromic sequence 5-TTGGCNNNNNGCCAA-3 present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication ...
Viruses or cells are targeted for selective internalization into a target in vive. A molecule Specific for a receptor on the surface of the target cell is introduced onto the surface of the virus or cell. The modified virus or cell binds the receptor in vive and is internalized by the target cell. The method provides vectors for selective delivery of nucleic acids to specific cell types in vivo and a means to alter the tropism of an infectious agent.
The adenovirus Elizabeth4orf4 protein induces non-classical apoptosis in mammalian cells through at least two complementing pathways regulated by the interactions of Age4orf4 with protein phosphatase 2A (PP2A) and Src kinases. to lead to the cytoplasmic loss of life function of Age4orf4. IMPORTANCE The adenovirus Age4orf4 proteins contributes to control of the development of pathogen disease …Read More. ...
E4-ORF3 clears the way for adenovirus to proliferate by deactivating genes that help the cell defend itself against the virus. This was discovered by OShea two years ago.
近藤 小貴 , 寺島 美保 , 福田 尋充 , 斎藤 泉 , 鐘ヶ江 裕美 ウイルス 57(1), 37-46, 2007-06-22 参考文献42件 ...
Here you can get all the birthday meanings for natives born under October 29 1956 horoscope explained through the astrology sign and Chinese zodiac animal.
Human enteric adenoviruses propagate poorly in conventional human cell lines used to grow other adenovirus serotypes. As human enteric adenoviruses have a defect in counteracting the cellular interferon (IFN) response in cell culture, to aid in growth of the virus, a 293-based cell line defective in its ability to respond to IFN was constructed. This cell line (293-SV5/V) constitutively expresses V-protein of the paramyxovirus Simian virus 5, which degrades the signal transducer and activator of transcription 1 (STAT1) and thereby prevents the STAT1-mediated IFN response. Analysis of human enteric adenovirus type 40 (HAdV-40)-infected 293-SV5/V cells compared with parental 293 cells shows that the recombinant line allows more rapid production of virus and results in higher titres. These results suggest that the defect in HAdV-40 in counteracting the IFN response can be overcome at least partially through the use of 293-SV5/V cell lines.. ...
RIGOTTO, C et al. Evaluation of HA negatively charged membranes in the recovery of human adenoviruses and hepatitis A virus in different water matrices. Mem. Inst. Oswaldo Cruz [online]. 2009, vol.104, n.7, pp.970-974. ISSN 0074-0276. http://dx.doi.org/10.1590/S0074-02762009000700005.. Human adenoviruses (HAdV) and hepatitis A virus (HAV) are shed in the faeces and consequently may be present in environmental waters, resulting in an increase in pathogen concentration that can affect water quality and human health. The aim of this study was to evaluate an adsorption-elution method which utilizes negatively charged membrane HA to determine the efficient recovery of HAdV and HAV from different water matrices and to combine this procedure with a qualitative molecular method (nested RT-PCR and nested PCR). The best efficiency recovery was achieved in distilled water and treated wastewater effluent (100%) for both viruses and in recreational lagoon water for HAV (100%). The efficiency recovery was 10% ...
Although epidemiologic characteristics of the adenoviruses vary by type, all are transmitted by direct contact, fecal-oral transmission, and occasionally waterborne transmission. Some types are capable of establishing persistent asymptomatic infections in tonsils, adenoids, and intestines of infected hosts, and shedding can occur for months or years. Some adenoviruses (e.g., serotypes 1, 2, 5, and 6) have been shown to be endemic in parts of the world where they have been studied, and infection is usually acquired during childhood. Other types cause sporadic infection and occasional outbreaks; for example, epidemic keratoconjunctivitis is associated with adenovirus serotypes 8, 19, and 37. Epidemics of febrile disease with conjunctivitis are associated with waterborne transmission of some adenovirus types, often centering on inadequately chlorinated swimming pools and small lakes. ARD is most often associated with adenovirus types 4 and 7 in the United States. Enteric adenoviruses 40 and 41 ...
Human adenovirus, of the Adenoviridae family, is a nonenveloped icosahedral particle containing a single linear dsDNA genome. Human adenovirus comprises 6 species (A through F) consisting of serotypes 1 - 51, and associated with a variety of clinical illnesses. Serotypes 1-39, 42-51 are associated with a variety of respiratory disease, generally in children and immunocompromised persons; whereas serotypes 40 and 41 is associated with enteric disease (enteric adenovirus) particularly in children - these enteric adenoviruses will not be detected with the assay described here. Serotypes 12, 18, 31 have a high oncogenic potential and serotypes 4 and 7 are associated with acute respiratory disease (ARD) frequently in military recruits and typically occur in the winter and spring. Smaller outbreaks of serotypes 3, 4, and 7 occur in the summertime and are associated with contaminated swimming pool water.. Adenovirus infections of the eye may lead to pharyngo-conjunctival fever, follicular ...
The fibre knob enables the virus to attach to the cellular receptor and, together with the hexon protein, defines the serological specificity of the adenoviruses. Therefore, the hexon gene and the fibre gene were analysed to compare the immunological data with the molecular biological results, in addition to looking for any possible variation that might be related to ocular pathogenicity. The hypervariable regions (HVRs) of the hexon gene and all regions of the fibre gene were sequenced by overlapping primers from genomic DNA by direct cycle sequencing. Multiple sets of primers for the hexon and fibre genes were selected based on alignment of the hexon gene (x76549 (Ad3), x76551 (Ad7), AB018424 (Ad11), AB018425 (Ad14), x74662 (Ad16), AY008279 (Ad21), AB018246 (Ad34), and AB018427 (Ad35)) and fibre gene sequences (m12411 (Ad3), m23696 (Ad7), L08231 (Ad11), AB065116 (Ad14), u06106 (Ad16), u06107 (Ad21), u10271 (Ad34), and u10272 (Ad35)) available from GeneBank. The sequences were determined by a ...
Human adenoviruses (HAdVs) cause a wide range of diseases worldwide, including respiratory infections. Studies on HAdV molecular epidemiology are limited in Cameroon. The purpose of this study is to document the different types HAdV circulating in Cameroon in children with acute respiratory infections. Nasopharyngeal swabs were collected from 811 children under 15 years from 2011 to 2014. The HAdV detection was assessed by semi-quantitative generic PCR r-gene®. The HAdV-positive samples were typed by amplification and sequencing of partial hexon gene and a real-time PCR. Demographic data were collected and analyzed. The infection and hospitalization risk factors were assessed thought the Chi-square test. A total of 137/220 HAdV-positive samples were amplified successfully. Six species of HAdV (Mastadenovirus A to F) were detected with B (108/220) and C (47/220) being the predominant strains. Hospitalization and age were significantly associated to HAdV-B and HAdV-C respectively. Phylogenetic analysis
Adenovirus type 37 (Ad37) is a leading cause of epidemic keratoconjunctivitis (EKC), a severe and highly contagious ocular disease. Whereas most other adenoviruses infect cells by engaging CD46 or the coxsackie and adenovirus receptor (CAR), Ad37 binds previously unknown sialic acid-containing cell surface molecules. By glycan array screening, we show here that the receptor-recognizing knob domain of the Ad37 fiber protein specifically binds a branched hexasaccharide that is present in the GD1a ganglioside and that features two terminal sialic acids. Soluble GD1a glycan and GD1a-binding antibodies efficiently prevented Ad37 virions from binding and infecting corneal cells. Unexpectedly, the receptor is constituted by one or more glycoproteins containing the GD1a glycan motif rather than the ganglioside itself, as shown by binding, infection and flow cytometry experiments. Molecular modeling, nuclear magnetic resonance and X-ray crystallography reveal that the two terminal sialic acids dock into two of
RNA molecules from nuclear and cytoplasmic polyribosomes of adenovirus-infected HeLa cells were compared by hybridization to analyse the sequence content. Nuclear polyribosomes were released by exposure of intact detergent-washed nuclei to poly(U) and purified. Cytoplasmic polyribosomes were also purified from the same cells. To show that nuclear polyribosomes contain ribosomes linked by mRNA, polyribosomes were labelled with methionine and uridine in the presence of actinomycin D in adenovirus-infected cells. Purified nuclear polyribosomes were treated with EDTA under conditions which dissociate polyribosomes into ribosomes and subunits with a simultaneous release of mRNA, and sedimented. The treatment dissociated these polyribosomes, releasing the mRNA from them. Radiolabelled total RNA from each polyribosome population was fractionated in sucrose gradients into several pools or hybridized to intact adenovirus DNA to select virus-specific RNA. Sucrose-gradient-fractionated pool-3 RNA (about ...
The utility of recombinant adenovirus serotype 5 (rAd5) vector-based vaccines for HIV-1 and other pathogens will likely be limited by the high prevalence of pre-existing Ad5-specific neutralizing Abs (NAbs) in human populations. However, the immunodominant targets of Ad5-specific NAbs in humans remain poorly characterized. In this study, we assess the titers and primary determinants of Ad5-specific NAbs in individuals from both the United States and the developing world. Importantly, median Ad5-specific NAb titers were ,10-fold higher in sub-Saharan Africa compared with the United States. Moreover, hexon-specific NAb titers were 4- to 10-fold higher than fiber-specific NAb titers in these cohorts by virus neutralization assays using capsid chimeric viruses. We next performed adoptive transfer studies in mice to evaluate the functional capacity of hexon- and fiber-specific NAbs to suppress the immunogenicity of a prototype rAd5-Env vaccine. Hexon-specific NAbs were remarkably efficient at ...
The high prevalence of pre-existing anti-Ad5 immunity in human populations may substantially limit the immunogenicity and clinical utility of rAd5 vector-based vaccines for HIV-1 and other pathogens. Our studies demonstrate ,90% Ad5 seroprevalence in sub-Saharan Africa with median NAb titers ,10-fold higher than those found in the United States. These data suggest that rAd5 vectors should be engineered to evade dominant Ad5-specific NAbs before their use as vaccine vectors in the developing world. To determine the principal targets of Ad5-specific NAbs, we exploited the lack of detectable serologic cross-reactivity between Ad5 and Ad35 (8). Virus neutralization studies using capsid chimeric rAd5/rAd35 vectors and serum samples from both humans and mice demonstrated that Ad5-specific NAbs were directed primarily against the Ad5 hexon protein. Fiber-specific NAbs were detected at low frequencies in vitro but were substantially less efficient than hexon-specific NAbs at blunting rAd5 vaccine ...
Introduction. Human adenoviruses (HAdVs) are important agents that cause serious infections in children and immunocompromised patients. HAdVs were first detected in military personnel with acute febrile respiratory disease. Later, clinical manifestations such as gastroenteritis, cystitis, hepatitis, keratoconjunctivitis, meningoencephalitis, and myocarditis were also related to these viruses.1-10. The epidemiological studies of adenovirus infections have limitations due to the relatively high incidence of doubtful and inconclusive results obtained in the tests currently in use. Direct immunofluorescence (IFD) and Enzyme Linked ImmunonoSorbent Assay (ELISA) assays are techniques that are not very sensitive for the diagnosis of adenovirus infections, compared to cell culture methods and molecular diagnosis.3-5,10. Virus isolation in cell cultures is a sensitive method for adenovirus detection, but this method is costly and time-consuming, taking several days to perform the isolation. As some ...
Introduction. Human adenoviruses (HAdVs) are important agents that cause serious infections in children and immunocompromised patients. HAdVs were first detected in military personnel with acute febrile respiratory disease. Later, clinical manifestations such as gastroenteritis, cystitis, hepatitis, keratoconjunctivitis, meningoencephalitis, and myocarditis were also related to these viruses.1-10. The epidemiological studies of adenovirus infections have limitations due to the relatively high incidence of doubtful and inconclusive results obtained in the tests currently in use. Direct immunofluorescence (IFD) and Enzyme Linked ImmunonoSorbent Assay (ELISA) assays are techniques that are not very sensitive for the diagnosis of adenovirus infections, compared to cell culture methods and molecular diagnosis.3-5,10. Virus isolation in cell cultures is a sensitive method for adenovirus detection, but this method is costly and time-consuming, taking several days to perform the isolation. As some ...
Last year, Chiu and colleagues also identified another new adenovirus, named simian adenovirus C, which sickened four of nine captive baboons and killed two of them at a primate facility in 1997. Several staff members at the facility also complained of upper respiratory symptoms at the time of the outbreak. Re-examining the samples many years later, Chiu and his colleagues found antibodies targeted to simian adenovirus C in the human samples.. Chiu concluded that staff members had been exposed to the new virus, and that the virus may have jumped from baboon to human, an idea also supported by follow-up experiments in which laboratory strains of simian adenovirus C efficiently infected both human and baboon cells.. "Adenoviruses to date have not generally been linked to cross-species infections between monkeys and humans," Chiu said.. In light of these findings, however, he said the normal vigilance in tracking animal viruses that might also infect humans should extend beyond influenza and ...
Seventy-eight cases were identified in patients from four eye care practices, two family practices, and the hospital emergency department. The median patient age was 45 years (range = 9 months-90 years), and 33 (42%) were men. Ocular signs and symptoms included redness (68%), watery discharge (50%), and pain (29%). Severe signs included corneal infiltrates (17%) and pseudomembranes (6%). At least 12 cases (15%) were health care-associated (Figure). One health care-associated case occurred in a health care worker. Seventeen patients whose infections were not health care-associated reported a symptomatic household or community contact. Among 45 conjunctival swabs available for testing, 19 (42%) were positive for HAdV-8. Genome sequences obtained from five HAdV-8 isolates were 100% identical with one another and showed 97.7% (accession number AB861610.1) to 99.9% (accession number KT340070.1) nucleotide sequence similarity to other HAdV-8 genome sequences available in GenBank, the National ...
Mature human adenovirus particles contain four minor capsid proteins, in addition to the three major capsid proteins (penton base, hexon and fiber) and several proteins associated with the genomic core of the virion. Of the minor capsid proteins, VI plays several crucial roles in the infection cycle of the virus, ... read more including hexon nuclear targeting during assembly, activation of the adenovirus proteinase (AVP) during maturation and endosome escape following cell entry. VI is translated as a precursor (pVI) that is cleaved at both N- and C-termini by AVP. Whereas the role of the C-terminal fragment of pVI, pVIc, is well established as an important co-factor of AVP, the role of the N-terminal fragment, pVIn, is currently elusive. In fact, the fate of pVIn following proteolytic cleavage is completely unknown. Here, we use a combination of proteomics-based peptide identification, native mass spectrometry and hydrogen-deuterium exchange mass spectrometry to show that pVIn is associated ...
Definition of Adenovirus E3 10.4K/14.5kD Protein in the Legal Dictionary - by Free online English dictionary and encyclopedia. What is Adenovirus E3 10.4K/14.5kD Protein? Meaning of Adenovirus E3 10.4K/14.5kD Protein as a legal term. What does Adenovirus E3 10.4K/14.5kD Protein mean in law?
3]Zhou C, Tian H, Wang X, Liu W, Yang S, Shen Q, Wang Y, Ni B, Chen S, Fu X, Fei R, Zhang W*.The genome sequence of a novel simian adenovirus in a chimpanzee reveals a close relationship to human adenoviruses.Arch Virol. 2014 Jul;159(7):1765-70. (Corresponding Author)影响因子IF=2.2 ...
Following receptor-mediated uptake into endocytic vesicles and escape from the endosome, adenovirus is transported by cytoplasmic dynein along microtubules to the perinuclear region of the cell. How motor proteins are recruited to viruses for their own use has begun to be investigated only recently. We review here the evidence for a role for dynein and other motor proteins in adenovirus infectivity. We also discuss the implications of recent studies on the mechanism of dynein recruitment to adenovirus for understanding the relationship between pathogenic and physiological cargo recruitment and for the evolutionary origins of dynein-mediated adenovirus transport.
The 293 cell line was derived from primary cultures of human embryonic kidney (HEK) cells with sheared fragments of adenovirus (Ad) 5 DNA (Graham et al. 1977). HEK 293 cells contain the nucleotides 1-4344 of Ad5 which are located within the pregnancy-specific ß-1-glycoprotein 4 (PSG 4) gene. The transforming region of the human adenovirus contains the early region (E1), comprising two transcription units, E1a and E1b, whose products are essential and sufficient for mammalian cell transformation by adenoviruses (Louis et al. 1997). Because 293 cells express E1 gene products they are extensively used for the production of E1-deleted Ad viruses. Adeno-associated viruses (AAVs) belong to the family of Parvoviridae, being one of the smallest single-stranded and non-enveloped DNA viruses. AAVss are replication-deficient and have required co-infection with a helper adeno- or herpes virus for productive infection. The AAV Helper-free system takes advantage of the identification of the specific ...
Adenoviruses are a group of medium sized, non enveloped, ds DNA viruses that share a common complement fixing antigen. They infect humans, animals and birds, shows strict host specifity. At least 47 serotypes of adenoviruses have been isolated from human sources. Adenovirus infections are common worldwide mostly in children. Many infections are asymptomatic. The virus may persist in the host for many months.It cause infections of the respiratory tract and eyes. These viruses carry DNA up to 7kb and are being investigated as potential vectors in gene therapy. ...
A new study explains how a genetically altered version of the adenovirus may improve the efficacy of mesothelioma chemotherapy drugs.
Background: The diagnosis of Kawasaki disease (KD) is often difficult to distinguish from HAdV. Objective: 1) To characterize the specific transcriptional profiles of KD patients versus acute HAdV infection 2) To determine whether the molecular distance to health (MDTH) score (a molecular score that reflects the perturbation derived from whole genome transcriptional analysis) correlates with response to therapy.. Methods: Whole blood RNA samples collected in Tempus tubes were analyzed using Illumina chips and GeneSpring software 7.4 from 76 pediatric patients with complete KD, 13 with incomplete KD, and 19 patients with HadV, and 20 age- and sex-matched healthy controls (HC). We used class comparison algorithms (Mann-Whitney p, 0.01, Benjamini-Hochberg, and 1.25- fold change filter) and modular analysis to define the KD profiles; class prediction algorithm was used to identify genes that best differentiate KD and HAdV.. Results: Statistical group comparisons identified 7,899 genes differentially ...
Adenoviruses were identified as unique viruses in 1953 by Rowe and coworkers when virus was isolated from human adenoids. Because adenoviruses infect various cells they are considered an excellent vector delivery system. A major obstacle to efficient infection by recombinant adenoviruses is the humo.... Full description. ...
Efforts to develop adenovirus vectors suitable for genetic interventions in humans have identified three major limitations of the most frequently used vector prototype, human adenovirus serotype 5 (Ad5). These limitations-widespread preexisting anti-Ad5 immunity in humans, the high rate of transduction of normal nontarget tissues, and the lack of target-specific gene delivery-justify the exploration of other Ad serotypes as vector prototypes. In this paper, we describe the development of an alternative vector platform using simian Ad serotype 24 (sAd24). We found that sAd24 virions formed unstable complexes with blood coagulation factor X and, because of that, transduced the liver and other organs at low levels when administered intravenously. The overall pattern of biodistribution of sAd24 particles was similar, however, to that of Ad5, and the intravenously injected sAd24 was cleared by Kupffer cells, leading to their depletion. We modified the viruss fiber protein to design a Her2-specific ...
Most adenoviruses use the coxsackie-adenovirus receptor (CAR) as a major cellular receptor. We have shown recently that adenovirus types 8, 19a, and 37, which are the major causes of epidemic keratoconjunctivitis, use sialic acid rather than CAR as a major cellular receptor. The predicted isoelectric point of the receptor-interacting knob domain in the adenovirus fiber protein is unusually high (9.0-9.1) in type 8, 19a, and 37. The pKa of sialic acid is low, 2.6, implying a possible involvement of charge in fiber knob-sialic acid interactions. Here we show that (i) positively charged adenovirus knobs require sialic acid for efficient cell membrane interactions; (ii) viral and knob interactions with immobilized sialic acid or cell-surface sialic acid are sensitive to increased ionic strength; (iii) negatively charged molecules such as sulfated glycosaminoglycans inhibit the binding of virions to target cells in a nonspecific, charge-dependent manner; and that (iv) the ability of adenovirus knobs ...
Looking for online definition of canine adenovirus type 2 (CAV-2) in the Medical Dictionary? canine adenovirus type 2 (CAV-2) explanation free. What is canine adenovirus type 2 (CAV-2)? Meaning of canine adenovirus type 2 (CAV-2) medical term. What does canine adenovirus type 2 (CAV-2) mean?
Link to Pubmed [PMID] - 30631958. Arch. Virol. 2019 Mar;164(3):747-755. A variety of viruses can cause acute flaccid paralysis (AFP). However, the causative agent, sometimes, remains undetermined. Metagenomics helps in identifying viruses not diagnosed by conventional methods. Stool samples from AFP (n = 104) and non-AFP (n = 114) cases that tested enterovirus-negative by WHO standard methods were investigated. A metagenomics approach, first used on five pools of four samples each, revealed the presence of adenovirus sequences. Amplification in A549 cells and full-genome sequencing were used for complete virus identification and for designing a PCR assay to screen individual related samples. Metagenomic analysis showed that adenovirus sequences that were closely to the A31 and A61 genotypes were the most abundant. Two out of the corresponding 20 individual samples were found positive by PCR, and isolates were obtained in cell culture. Phylogenetic analysis based on complete genome sequences ...
April 12, 2013 / 62(14);267-269. Baits laden with oral rabies vaccines are important for the management of wildlife rabies in the United States (1). In August 2012, the Wildlife Services program of the U.S. Department of Agricultures Animal and Plant Health Inspection Service began a field trial involving limited distribution of a new oral rabies vaccine bait in five states, including Ohio. The vaccine consisted of live recombinant human adenovirus type 5 vector, expressing rabies virus glycoprotein (AdRG1.3) (Onrab). A previously used oral rabies vaccine consisting of a live recombinant vaccinia vector, expressing rabies virus glycoprotein (V-RG) (Raboral V-RG) (2,3), was distributed in other areas of Ohio. To monitor human contacts and potential vaccine virus exposure, surveillance was conducted by the Ohio Department of Health, local Ohio health agencies, and CDC. During August 23-September 7, 2012, a total of 776,921 baits were distributed in Ohio over 4,379 square miles (11,341 square ...
Define epidemic keratoconjunctivitis. epidemic keratoconjunctivitis synonyms, epidemic keratoconjunctivitis pronunciation, epidemic keratoconjunctivitis translation, English dictionary definition of epidemic keratoconjunctivitis. n. Inflammation of the cornea and conjunctiva. Noun 1. keratoconjunctivitis - inflammation of the cornea and conjunctiva inflammation, redness, rubor - a...
An adenovirus type-2 was isolated from peripheral blood lymphocytes and throat washings from a patient with severe chronic active Epstein-Barr virus infection. Despite the Epstein-Barr virus reactivation, attempts to establish spontaneous lymphoblastoid cell lines from peripheral blood lymphocytes and to immortalize cord lymphocytes with throat washings were unsuccessful due to a marked cytopathic effect. The supernatants from the cultures induced cytopathic effect in cultured cord lymphocytes, MRC-5 cells, A-549 cells, or Vero cells. Virus particles with adenovirus morphology were seen by electron microscopy. Using type-specific antisera, the isolate was identified as adenovirus type-2. In addition, both Epstein-Barr virus and adenovirus type-2 genomes were seen in the colonic tissues and spleen. These results suggest that the combination of Epstein-Barr virus and adenovirus type-2 may be etiologic agents in the development of chronic active Epstein-Barr virus infection in this patient. ...
TY - JOUR. T1 - Concurrent acute necrotizing adenovirus hepatitis and enterocolitis in an adult patient after double cord blood stem cell transplant for refractory. AU - Disease, Crohns. AU - Lo, Amy A.. AU - Lo, Edward C.. AU - Rao, M. Sambasivia. AU - Yang, Guang Yu. PY - 2015/5/1. Y1 - 2015/5/1. N2 - It has been recently recognized that adenovirus is a pathogen with high morbidity and mortality among immunocompromised patients, particularly after solid organ or stem cell transplant. Confluent necrotizing hepatitis secondary to adenovirus infection alone or together with other organ involvement is extremely rare. There are only 32 cases of confluent necrotizing hepatitis reported in adults since 1960 and most occur after iatrogenic immunosuppression for bone marrow or solid organ transplantation or in other states of immunosuppression, including acquired immunodeficiency syndrome or chemotherapy treatment. We present the first case of concurrent adenovirus-induced necrotizing hepatitis and ...

TACE Plus Recombinant Human Adenovirus for Hepatocellular Carcinoma - Tabular View - ClinicalTrials.govTACE Plus Recombinant Human Adenovirus for Hepatocellular Carcinoma - Tabular View - ClinicalTrials.gov

Drug: Recombinant Human Adenovirus Type 5 Injection After identifying the target artery of HCC, Recombinant Human Adenovirus ... Recombinant Human Adenovirus Type 5, an E1B gene deleted adenovirus, is known to have a significant antitumor activity. In ... Experimental: TACE Plus Adenovirus After identifying the target artery of HCC, Recombinant Human Adenovirus Type 5 Injection( ... TACE Plus Recombinant Human Adenovirus for Hepatocellular Carcinoma. Official Title ICMJE Phase Ⅲ Trial of Transcatheter ...
more infohttps://clinicaltrials.gov/ct2/show/record/NCT01869088

Epidemic keratoconjunctivitis - definition of epidemic keratoconjunctivitis by The Free DictionaryEpidemic keratoconjunctivitis - definition of epidemic keratoconjunctivitis by The Free Dictionary

Outbreak of Epidemic Keratoconjunctivitis Caused by Human Adenovirus Type D53 in an Eye Care Clinic--Los Angeles County, 2017 ... Evidence of molecular evolution driven by recombination events influencing tropism in a novel human adenovirus that causes ... An outbreak of epidemic keratoconjunctivitis caused by a new intermediate adenovirus 22/H8 identified by molecular typing.. ... Epidemic keratoconjunctivitis is the severest ocular disease caused by adenoviruses (3) Ad 8, 9 and 37 are the serotypes most ...
more infohttps://www.thefreedictionary.com/epidemic+keratoconjunctivitis

What is the focus of clinical history in the evaluation of viral conjunctivitis (pink eye)?What is the focus of clinical history in the evaluation of viral conjunctivitis (pink eye)?

Novel human adenovirus causing nosocomial epidemic keratoconjunctivitis. J Clin Microbiol. 2008 Jun. 46(6):2002-8. [Medline]. ... The possibility of human adenovirus detection from the conjunctiva in asymptomatic cases during nosocomial infection. Cornea. ... Kimura R, Migita H, Kadonosono K, Uchio E. Is it possible to detect the presence of adenovirus in conjunctiva before the onset ... Sensitivity and specificity of the AdenoPlus point-of-care system in detecting adenovirus in conjunctivitis patients at an ...
more infohttps://www.medscape.com/answers/1191370-42941/what-is-the-focus-of-clinical-history-in-the-evaluation-of-viral-conjunctivitis-pink-eye

What should be included in the history of a patient with viral conjunctivitis (pink eye)?What should be included in the history of a patient with viral conjunctivitis (pink eye)?

Novel human adenovirus causing nosocomial epidemic keratoconjunctivitis. J Clin Microbiol. 2008 Jun. 46(6):2002-8. [Medline]. ... The possibility of human adenovirus detection from the conjunctiva in asymptomatic cases during nosocomial infection. Cornea. ... Kimura R, Migita H, Kadonosono K, Uchio E. Is it possible to detect the presence of adenovirus in conjunctiva before the onset ... Sensitivity and specificity of the AdenoPlus point-of-care system in detecting adenovirus in conjunctivitis patients at an ...
more infohttps://www.medscape.com/answers/1191370-42942/what-should-be-included-in-the-history-of-a-patient-with-viral-conjunctivitis-pink-eye

Ebola Vaccine Market 2019 Size, Market Share, Status, SWOT Analysis and Forecast to 2028 - PharmiWeb.comEbola Vaccine Market 2019 Size, Market Share, Status, SWOT Analysis and Forecast to 2028 - PharmiWeb.com

After the Ebola virus infection/attack human body shows symptoms - high fever, sore thr... ... Chimpanzee Adeno virus type 3-Zaire Ebola virus. Recombinant vesicular stomatitis virus-Zaire Ebola virus. Adeno virus serotype ... After the Ebola virus infection/attack human body shows symptoms - high fever, sore throat, vomiting, diarrhea, muscular pain, ...
more infohttps://www.pharmiweb.com/press-release/2019-03-29/ebola-vaccine-market-2019-size-market-share-status-swot-analysis-and-forecast-to-2028

Viral Conjunctivitis Drugs Market 2020: America, EMEA & APAC Regions Analyzed Report: Sandlerresearch.org - Industry Research...Viral Conjunctivitis Drugs Market 2020: America, EMEA & APAC Regions Analyzed Report: Sandlerresearch.org - Industry Research...

Viral conjunctivitis, or pink eye, is a type of infectious conjunctivitis that is commonly caused by an adenovirus. Herpes ... and human immunodeficiency virus (HIV) are some of the other viruses that are responsible for the infection. Discomfort through ...
more infohttps://pitchengine.com/pitches/6cc3e9b6-792c-4e58-a322-3a08e4c4ea4c

Canine Adenovirus Pneumonia in 2 puppies- CAV-2 | veterinary pathologyCanine Adenovirus Pneumonia in 2 puppies- CAV-2 | veterinary pathology

Canine Adenovirus Pneumonia in 2 puppies- CAV-2 History: Two English Bulldog puppies (1, and 3 weeks old) had trouble breathing ... Human Infectious Disease Pathology. *Human Pathology. *Ocular Pathology- Wisconsin COPLOW. *Path Wonk- Neuro/Forensic Human ... Canine Adenovirus Pneumonia in 2 puppies- CAV-2. Posted on October 27, 2011 by Brian ... Canine Adenovirus Pneumonia in 2 puppies- CAV-2. History: Two English Bulldog puppies (1, and 3 weeks old) had trouble ...
more infohttps://vetpath.wordpress.com/2011/10/27/canine-adenovirus-pneumonia-in-2-puppies-cav-2/

ACUTE UPPER RESPIRATORY TRACT INFECTION-[Good Tcm Net]ACUTE UPPER RESPIRATORY TRACT INFECTION-[Good Tcm Net]

The causes of this disorder are adenovirus, Coxsackie and influenza A, B and C viruses, and it is mainly characterized by fever ... Breeding-human-sperm Male-infertility Male-fertility Chronic-colitis colitis Colon Colon-cancer Prostate-cancer ... Most cases are caused by viruses such as rhinovirus, para influenza, respiratory syncytial virus, adenovirus, influenza A, B ...
more infohttps://www.tcmwell.com/SinoWesternMedicineJoint/ACUTE-UPPER-RESPIRATORY-TRACT-INFECTION.html

Human Adenovirus Surveillance - United States, 2003-2016  | MMWRHuman Adenovirus Surveillance - United States, 2003-2016 | MMWR

National Adenovirus Type Reporting System identifies circulation patterns for human adenovirus. ... National Adenovirus Type Reporting System identifies circulation patterns for human adenovirus. ... Distribution of human adenovirus species (HAdVs) and types, by year of specimen collection* - National Adenovirus Type ... The figure above is a bar chart showing the distribution of human adenovirus species and types by the National Adenovirus Type ...
more infohttps://www.cdc.gov/mmwr/volumes/66/wr/mm6639a2.htm

Human adenovirus 12 ATCC ® VR-863D™Human adenovirus 12 ATCC ® VR-863D™

DNA from Human adenovirus 12 strain Huie [ATCC ® VR-863™] Application: It is suitable for use in polymerase chain reaction (PCR ... DNA from Human adenovirus 12 strain Huie [ATCC® VR-863™] (ATCC® VR-863D™) Organism: Human adenovirus 12 / Focus: Infectious ... infected with Human adenovirus 12 (HAdV-12) strain Huie (ATCC® VR-863™). It is supplied at 100 µL per vial, shipped frozen. It ...
more infohttps://www.atcc.org/en/Products/Nucleic_Acid_Proteins_and_Cell_Extracts/Genomic_DNA_and_RNA/From_Virus/VR-863D.aspx?p=1&rel=%7B0%7D&slp=1

Human adenovirus 12 ATCC ® VR-863D™Human adenovirus 12 ATCC ® VR-863D™

DNA from Human adenovirus 12 strain Huie [ATCC ® VR-863™] Application: It is suitable for use in polymerase chain reaction (PCR ... DNA from Human adenovirus 12 strain Huie [ATCC® VR-863™] (ATCC® VR-863D™) Organism: Human adenovirus 12 / Focus: Infectious ... DNA from Human adenovirus 12 strain Huie [ATCC® VR-863™] ATCC® VR-863D™ frozen 100 µL per vial ... infected with Human adenovirus 12 (HAdV-12) strain Huie (ATCC® VR-863™). It is supplied at 100 µL per vial, shipped frozen. It ...
more infohttps://www.atcc.org/Products/All/VR-863D.aspx

Pharmaceuticals  | Free Full-Text | Anti-Viral Drugs for Human Adenoviruses | HTMLPharmaceuticals | Free Full-Text | Anti-Viral Drugs for Human Adenoviruses | HTML

This review attempts to address the importance of developing pharmaceuticals for adenovirus and also review recent development ... in drug discovery for adenovirus, including newer strategies such as microRNA approaches. Different drug screening strategies ... many stages in the development of a new drug for viral infection and such processes are even further complicated for adenovirus ... Adenovirus Biology and Structure. An adenovirus is a non-enveloped, dsDNA virus. There are at least 51 human Adenovirus ...
more infohttp://www.mdpi.com/1424-8247/3/10/3343/htm

TAT Adenovirus (Human) - Gentaur.comTAT Adenovirus (Human) - Gentaur.com

Human) product information; TAT Adenovirus (Human) is available 1 time from supplier abm Adinovirus at Gentaur.com shop ... TAT Adenovirus (Human). TAT Adenovirus (Human) is available 1 time from Abm adinovirus labs 137793A , TAT Adenovirus (Human) ... STAT3-luc Adenovirus (Human) Suppplier: abm Adinovirus Price: 490.09 USD STAT2-HA Adenovirus (Human) Suppplier: abm Adinovirus ... STAT6 Adenovirus (Human) Suppplier: abm Adinovirus Price: 490.09 USD TAT-His Adenovirus (Human) Suppplier: abm Adinovirus Price ...
more infohttps://gentaur.com/871905482/tat-adenovirus-human/abm-adinovirus?p=1014531520

Human adenovirus A serotype 12 (HAdV-12) (Human adenovirus 12)Human adenovirus A serotype 12 (HAdV-12) (Human adenovirus 12)

Human adenovirus 12. Other names i. ›Adenovirus Ad12. ›Adenovirus type 12. ›Human adenovirus type 12. ›Mastadenovirus 12. › ... Taxonomy - Human adenovirus A serotype 12 (HAdV-12) (Human adenovirus 12) Basket 0 ...
more infohttps://www.uniprot.org/taxonomy/28282

MAMLD1 Adenovirus (Human) - Gentaur.comMAMLD1 Adenovirus (Human) - Gentaur.com

Human) product information; MAMLD1 Adenovirus (Human) is available 1 time from supplier abm Adinovirus at Gentaur.com shop ... MAMLD1 Adenovirus (Human). MAMLD1 Adenovirus (Human) is available 1 time from Abm adinovirus labs. 105951A , MAMLD1 Adenovirus ... Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal ... Before using MAMLD1 Adenovirus (Human) please read the package insert. It is intended for Human reactivity. Accession Number: ...
more infohttps://gentaur.com/prod/mamld1-adenovirus-human/abm-adinovirus/995799015

IX (Human adenovirus F) | Gene Target - PubChemIX (Human adenovirus F) | Gene Target - PubChem

Human adenovirus F). Find diseases associated with this biological target and compounds tested against it in bioassay ...
more infohttps://pubchem.ncbi.nlm.nih.gov/target/gene/ix

TACE Plus Recombinant Human Adenovirus for Hepatocellular Carcinoma - Full Text View - ClinicalTrials.govTACE Plus Recombinant Human Adenovirus for Hepatocellular Carcinoma - Full Text View - ClinicalTrials.gov

Drug: Recombinant Human Adenovirus Type 5 Injection After identifying the target artery of HCC, Recombinant Human Adenovirus ... Recombinant Human Adenovirus Type 5, an E1B gene deleted adenovirus, is known to have a significant antitumor activity. In ... Experimental: TACE Plus Adenovirus After identifying the target artery of HCC, Recombinant Human Adenovirus Type 5 Injection( ... TACE Plus Recombinant Human Adenovirus for Hepatocellular Carcinoma. The safety and scientific validity of this study is the ...
more infohttps://clinicaltrials.gov/ct2/show/NCT01869088?cond=%22Viral+Infectious+Disease%22&intr=%22Ethanol%22&rank=73

Rapid and quantitative detection of human adenovirus DNA by real-time PCR.  - PubMed - NCBIRapid and quantitative detection of human adenovirus DNA by real-time PCR. - PubMed - NCBI

Rapid and quantitative detection of human adenovirus DNA by real-time PCR.. Heim A1, Ebnet C, Harste G, Pring-Akerblom P. ... Rapid diagnosis of human adenovirus (HAdV) infections was achieved by PCR in the recent years. However, conventional PCR has ... Adenovirus viremia was detected by TaqMan PCR in 4 of 27 (14.8%) paediatric and 8 of 93 (8.6%) adult stem cell transplant ... In conclusion, real-time PCR is a sensitive and quantitative procedure for the detection of adenovirus infections. ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/12696109?dopt=Abstract

Viral evolution revealed by bacteriophage PRD1 and human adenovirus coat protein structures.  - PubMed - NCBIViral evolution revealed by bacteriophage PRD1 and human adenovirus coat protein structures. - PubMed - NCBI

Viral evolution revealed by bacteriophage PRD1 and human adenovirus coat protein structures.. Benson SD1, Bamford JK, Bamford ... Surprisingly, the P3 molecule closely resembles hexon, the equivalent protein in human adenovirus. Both viruses also have ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/10499799?dopt=Abstract

Genetic diversity of the human adenovirus species C DNA polymeraseGenetic diversity of the human adenovirus species C DNA polymerase

Human Adenovirus (HAdV) are responsible for severe infections in hematopoietic stem cells transplant (HSCT) recipient, species ... Background:Human Adenovirus (HAdV) are responsible for severe infections in hematopoietic stem cells transplant (HSCT) ...
more infohttps://insights.ovid.com/antvre/201808000/00078401-201808000-00001

Functional characterization of the human adenovirus pVII protein and non-coding VA RNAIFunctional characterization of the human adenovirus pVII protein and non-coding VA RNAI

2. Human adenovirus infection counteracts the anti-viral activity of the cellular MKRN1 E3 ubiquitin ligase. Open this ... Human adenovirus (HAdV) is a common pathogen causing a broad spectrum of diseases. HAdV encodes the pVII protein, which is ... 4. RNA triplex formation in human adenovirus type 4 VA RNAI and its implication on virus growth. Open this publication in new ... Open this publication in new window or tab ,,Complementation of the human adenovirus type 5 VA RNAI defect by the Vaccinia ...
more infohttp://www.diva-portal.org/smash/record.jsf?pid=diva2:1094348

L5 - Fiber protein - Human adenovirus B serotype 3 (HAdV-3) - L5 gene & proteinL5 - Fiber protein - Human adenovirus B serotype 3 (HAdV-3) - L5 gene & protein

Human adenovirus B serotype 3 (HAdV-3) (Human adenovirus 3). ,p>This subsection of the ,a href="http://www.uniprot.org/help/ ... sp,P04501,SPIKE_ADE03 Fiber protein OS=Human adenovirus B serotype 3 OX=45659 GN=L5 PE=1 SV=1 ... IPR008982 Adenovirus_pIV-like_att. IPR009013 Attachment_protein_shaft_sf. Pfami. View protein in Pfam. PF00541 Adeno_knob, 1 ... Viruses › dsDNA viruses, no RNA stage › Adenoviridae › Mastadenovirus › Human mastadenovirus B. ,p>This subsection of the ,a ...
more infohttps://www.uniprot.org/uniprot/P04501

Human adenovirus meningoencephalitis: a 3-years overview | Springer for Research & DevelopmentHuman adenovirus meningoencephalitis: a 3-years' overview | Springer for Research & Development

Human adenovirus (HAdV) has been recognized as a significant viral pathogen implicated in neurological diseases, particularly ... Human adenovirus (HAdV) has been recognized as a significant viral pathogen implicated in neurological diseases, particularly ... Avellón A, Pérez P, Aguilar JC, Lejarazu R, Echevarrı́a JE (2001) Rapid and sensitive diagnosis of human adenovirus infections ... of clinical adenovirus specimens by an algorithm which permits detection of adenovirus coinfection and intermediate adenovirus ...
more infohttps://rd.springer.com/article/10.1007%2Fs13365-019-00758-7

Molecular typing of human adenoviruses by PCR and sequencing of a partial region of the hexon gene | SpringerLinkMolecular typing of human adenoviruses by PCR and sequencing of a partial region of the hexon gene | SpringerLink

Human adenoviruses (Ads) are responsible for a substantial disease burden. Type-specific identification of Ads can help guide ... Gruber, WC, Russell, DJ, Tibbetts, C 1993Fiber gene and genomic origin of human adenovirus type 4Virology196603611PubMed ... Shimada, Y, Ariga, T, Yoshitsugu, T, Aoki, K, Ohno, S, Ishiko, H 2004Molecular diagnosis of human adenoviruses D and E by a ... Pring-Åkerblom, P, Adrian, T, Kostler, T 1997PCR-based detection and typing of human adenoviruses in clinical samplesRes Virol ...
more infohttps://link.springer.com/article/10.1007%2Fs00705-005-0722-7
  • Coxsackievirus and adenovirus receptor, CD46, sialic acid, coagulation factors IX and X, lactoferrin and heparan sulfate are some receptors and molecules which the hexon and fiber proteins (components of the capsid) bind for direct or indirect cellular attachment. (diva-portal.org)
  • By using a previously described DNA restriction analysis procedure ( 7 ), we studied 76 archived adenovirus isolates collected among influenzalike-illness surveillance sites across Iowa from 1992 to 2002. (cdc.gov)
  • Hybridization measurements among the DNA's of six weakly tumorigenic strains of human adenovirus type 7 reveal that all six strains are very closely related (83- to 110-percent nucleotide sequence homology). (sciencemag.org)
  • Since Ad7d2 has been associated with 3 military and 3 civilian epidemics and at least 19 deaths in the United States since 1993, the 2002 report voiced concern regarding a shift in the prevalence of U.S. adenovirus strains and the need to increase surveillance for adenoviral disease. (cdc.gov)
  • Recently, molecular methods have shown adenoviruses to be associated with bronchopulmonary dysplasia ( 1 ), chronic obstructive pulmonary disease ( 2 ), and mycocarditis ( 3 ). (cdc.gov)
  • Adenoviruses have been detected in raw sewage in relatively high frequencies ( 22 , 25 ), and they were found in environmental sources, including Southern California coastal waters, using molecular methods ( 23 , 33 ). (asm.org)
  • He played a critical role in developing adenovirus as an experimental system and made many important contributions in virology and molecular biology, leading to over 300 authored/co-authored publications and one U.S. patent (No. 61/509,891). (wikipedia.org)
  • The prevalence of adenovirus was not significantly different between rainy and dry seasons. (uib.no)
  • We found similar prevalence of adenovirus in non-diarrhoeic children and in diarrhoeic children. (uib.no)
  • In this approach, macrophages were cotransduced with a hypoxia-regulated E1A/B construct and an E1A-dependent oncolytic adenovirus, whose proliferation is restricted to prostate tumor cells using prostate-specific promoter elements from the TARP, PSA, and PMSA genes. (aacrjournals.org)
  • Here we describe the development of two real-time PCR methods to detect and quantify human adenoviruses and enterococci in environmental waters. (asm.org)