Species of the genus MASTADENOVIRUS, causing a wide range of diseases in humans. Infections are mostly asymptomatic, but can be associated with diseases of the respiratory, ocular, and gastrointestinal systems. Serotypes (named with Arabic numbers) have been grouped into species designated Human adenovirus A-F.
A family of non-enveloped viruses infecting mammals (MASTADENOVIRUS) and birds (AVIADENOVIRUS) or both (ATADENOVIRUS). Infections may be asymptomatic or result in a variety of diseases.
Respiratory and conjunctival infections caused by 33 identified serotypes of human adenoviruses.
Proteins transcribed from the E1A genome region of ADENOVIRUSES which are involved in positive regulation of transcription of the early genes of host infection.
Virus diseases caused by the ADENOVIRIDAE.
Proteins encoded by adenoviruses that are synthesized prior to, and in the absence of, viral DNA replication. The proteins are involved in both positive and negative regulation of expression in viral and cellular genes, and also affect the stability of viral mRNA. Some are also involved in oncogenic transformation.
Proteins transcribed from the E1B region of ADENOVIRUSES which are involved in regulation of the levels of early and late viral gene expression.
Proteins transcribed from the E3 region of ADENOVIRUSES but not essential for viral replication. The E3 19K protein mediates adenovirus persistence by reducing the expression of class I major histocompatibility complex antigens on the surface of infected cells.
Proteins transcribed from the E4 region of ADENOVIRUSES. The E4 19K protein transactivates transcription of the adenovirus E2F protein and complexes with it.
The very first viral gene products synthesized after cells are infected with adenovirus. The E1 region of the genome has been divided into two major transcriptional units, E1A and E1B, each expressing proteins of the same name (ADENOVIRUS E1A PROTEINS and ADENOVIRUS E1B PROTEINS).
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
Species of the genus MASTADENOVIRUS that causes fever, edema, vomiting, and diarrhea in dogs and encephalitis in foxes. Epizootics have also been caused in bears, wolves, coyotes, and skunks. The official species name is Canine adenovirus and it contains two serotypes.
Proteins transcribed from the E2 region of ADENOVIRUSES. Several of these are required for viral DNA replication.
A genus of ADENOVIRIDAE that infects MAMMALS including humans and causes a wide range of diseases. The type species is Human adenovirus C (see ADENOVIRUSES, HUMAN).
Species of the genus MASTADENOVIRUS, causing neurological disease in pigs.
A genus of ADENOVIRIDAE that infects birds. The type species is FOWL ADENOVIRUS A.
Techniques and strategies which include the use of coding sequences and other conventional or radical means to transform or modify cells for the purpose of treating or reversing disease conditions.
The type species of the genus AVIADENOVIRUS, family ADENOVIRIDAE, an oncogenic virus of birds. This is also called CELO virus for chick embryo lethal orphan virus.
Deoxyribonucleic acid that makes up the genetic material of viruses.
The introduction of functional (usually cloned) GENES into cells. A variety of techniques and naturally occurring processes are used for the gene transfer such as cell hybridization, LIPOSOMES or microcell-mediated gene transfer, ELECTROPORATION, chromosome-mediated gene transfer, TRANSFECTION, and GENETIC TRANSDUCTION. Gene transfer may result in genetically transformed cells and individual organisms.
Proteins that form the CAPSID of VIRUSES.
The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
Established cell cultures that have the potential to propagate indefinitely.
The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.
Specific molecular components of the cell capable of recognizing and interacting with a virus, and which, after binding it, are capable of generating some signal that initiates the chain of events leading to the biological response.
Use of attenuated VIRUSES as ANTINEOPLASTIC AGENTS to selectively kill CANCER cells.
Tumor-selective, replication competent VIRUSES that have antineoplastic effects. This is achieved by producing cytotoxicity-enhancing proteins and/or eliciting an antitumor immune response. They are genetically engineered so that they can replicate in CANCER cells but not in normal cells, and are used in ONCOLYTIC VIROTHERAPY.
Simultaneous inflammation of the cornea and conjunctiva.
The transfer of bacterial DNA by phages from an infected bacterium to another bacterium. This also refers to the transfer of genes into eukaryotic cells by viruses. This naturally occurring process is routinely employed as a GENE TRANSFER TECHNIQUE.
Proteins found in any species of virus.
The functional hereditary units of VIRUSES.
Products of viral oncogenes, most commonly retroviral oncogenes. They usually have transforming and often protein kinase activities.
Inflammation, often mild, of the conjunctiva caused by a variety of viral agents. Conjunctival involvement may be part of a systemic infection.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Vaccines used to prevent infection by any virus from the family ADENOVIRIDAE.
The outer protein protective shell of a virus, which protects the viral nucleic acid.
An inheritable change in cells manifested by changes in cell division and growth and alterations in cell surface properties. It is induced by infection with a transforming virus.
Genes that are introduced into an organism using GENE TRANSFER TECHNIQUES.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Visible morphologic changes in cells infected with viruses. It includes shutdown of cellular RNA and protein synthesis, cell fusion, release of lysosomal enzymes, changes in cell membrane permeability, diffuse changes in intracellular structures, presence of viral inclusion bodies, and chromosomal aberrations. It excludes malignant transformation, which is CELL TRANSFORMATION, VIRAL. Viral cytopathogenic effects provide a valuable method for identifying and classifying the infecting viruses.
Viruses which enable defective viruses to replicate or to form a protein coat by complementing the missing gene function of the defective (satellite) virus. Helper and satellite may be of the same or different genus.
Viruses which lack a complete genome so that they cannot completely replicate or cannot form a protein coat. Some are host-dependent defectives, meaning they can replicate only in cell systems which provide the particular genetic function which they lack. Others, called SATELLITE VIRUSES, are able to replicate only when their genetic defect is complemented by a helper virus.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.
Ribonucleic acid that makes up the genetic material of viruses.
Any of the processes by which cytoplasmic factors influence the differential control of gene action in viruses.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
A genus of the family PICORNAVIRIDAE whose members preferentially inhabit the intestinal tract of a variety of hosts. The genus contains many species. Newly described members of human enteroviruses are assigned continuous numbers with the species designated "human enterovirus".
The process by which a DNA molecule is duplicated.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
A genus of ADENOVIRIDAE that comprises viruses of several species of MAMMALS and BIRDS. The type species is Ovine adenovirus D.
A species of POLYOMAVIRUS originally isolated from Rhesus monkey kidney tissue. It produces malignancy in human and newborn hamster kidney cell cultures.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
Mutant mice homozygous for the recessive gene "nude" which fail to develop a thymus. They are useful in tumor studies and studies on immune responses.
Process of growing viruses in live animals, plants, or cultured cells.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
The complete genetic complement contained in a DNA or RNA molecule in a virus.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
A cell line derived from cultured tumor cells.
Invasion of the host RESPIRATORY SYSTEM by microorganisms, usually leading to pathological processes or diseases.
Production of new arrangements of DNA by various mechanisms such as assortment and segregation, CROSSING OVER; GENE CONVERSION; GENETIC TRANSFORMATION; GENETIC CONJUGATION; GENETIC TRANSDUCTION; or mixed infection of viruses.
Immunoglobulins produced in response to VIRAL ANTIGENS.
Minute infectious agents whose genomes are composed of DNA or RNA, but not both. They are characterized by a lack of independent metabolism and the inability to replicate outside living host cells.
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Those proteins recognized by antibodies from serum of animals bearing tumors induced by viruses; these proteins are presumably coded for by the nucleic acids of the same viruses that caused the neoplastic transformation.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
Process of determining and distinguishing species of bacteria or viruses based on antigens they share.
An area showing altered staining behavior in the nucleus or cytoplasm of a virus-infected cell. Some inclusion bodies represent "virus factories" in which viral nucleic acid or protein is being synthesized; others are merely artifacts of fixation and staining. One example, Negri bodies, are found in the cytoplasm or processes of nerve cells in animals that have died from rabies.
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
Inflammation of the lung parenchyma that is caused by a viral infection.
A suborder of PRIMATES consisting of six families: CEBIDAE (some New World monkeys), ATELIDAE (some New World monkeys), CERCOPITHECIDAE (Old World monkeys), HYLOBATIDAE (gibbons and siamangs), CALLITRICHINAE (marmosets and tamarins), and HOMINIDAE (humans and great apes).
Substances elaborated by viruses that have antigenic activity.
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
A ubiquitously expressed complement receptor that binds COMPLEMENT C3B and COMPLEMENT C4B and serves as a cofactor for their inactivation. CD46 also interacts with a wide variety of pathogens and mediates immune response.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Eukaryotic cell line obtained in a quiescent or stationary phase which undergoes conversion to a state of unregulated growth in culture, resembling an in vitro tumor. It occurs spontaneously or through interaction with viruses, oncogenes, radiation, or drugs/chemicals.
A genus of the family PARVOVIRIDAE, subfamily PARVOVIRINAE, which are dependent on a coinfection with helper adenoviruses or herpesviruses for their efficient replication. The type species is Adeno-associated virus 2.
Suspensions of attenuated or killed viruses administered for the prevention or treatment of infectious viral disease.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
INFLAMMATION of any segment of the GASTROINTESTINAL TRACT from ESOPHAGUS to RECTUM. Causes of gastroenteritis are many including genetic, infection, HYPERSENSITIVITY, drug effects, and CANCER.
This line KB is now known to be a subline of the ubiquitous KERATIN-forming tumor cell line HeLa. It was originally thought to be derived from an epidermal carcinoma of the mouth, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via contamination by HELA CELLS. The cells are positive for keratin by immunoperoxidase staining. KB cells have been reported to contain human papillomavirus18 (HPV-18) sequences.
Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected.
Viruses that produce tumors.
The directional growth of an organism in response to an external stimulus such as light, touch, or gravity. Growth towards the stimulus is a positive tropism; growth away from the stimulus is a negative tropism. (From Concise Dictionary of Biology, 1990)
Injections introduced directly into localized lesions.
An enzyme that catalyzes the conversion of ATP and thymidine to ADP and thymidine 5'-phosphate. Deoxyuridine can also act as an acceptor and dGTP as a donor. (From Enzyme Nomenclature, 1992) EC 2.7.1.21.
In vivo methods of screening investigative anticancer drugs, biologic response modifiers or radiotherapies. Human tumor tissue or cells are transplanted into mice or rats followed by tumor treatment regimens. A variety of outcomes are monitored to assess antitumor effectiveness.
Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH.
The genetic unit consisting of three structural genes, an operator and a regulatory gene. The regulatory gene controls the synthesis of the three structural genes: BETA-GALACTOSIDASE and beta-galactoside permease (involved with the metabolism of lactose), and beta-thiogalactoside acetyltransferase.
A subfamily of the family MURIDAE comprised of 69 genera. New World mice and rats are included in this subfamily.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
Proteins prepared by recombinant DNA technology.
The genetic process of crossbreeding between genetically dissimilar parents to produce a hybrid.
The measurement of infection-blocking titer of ANTISERA by testing a series of dilutions for a given virus-antiserum interaction end-point, which is generally the dilution at which tissue cultures inoculated with the serum-virus mixtures demonstrate cytopathology (CPE) or the dilution at which 50% of test animals injected with serum-virus mixtures show infectivity (ID50) or die (LD50).
Small synthetic peptides that mimic surface antigens of pathogens and are immunogenic, or vaccines manufactured with the aid of recombinant DNA techniques. The latter vaccines may also be whole viruses whose nucleic acids have been modified.
Cell changes manifested by escape from control mechanisms, increased growth potential, alterations in the cell surface, karyotypic abnormalities, morphological and biochemical deviations from the norm, and other attributes conferring the ability to invade, metastasize, and kill.
Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Tumors or cancer of the MOUTH.
Elements of limited time intervals, contributing to particular results or situations.
Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.
A general term for diseases produced by viruses.
Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
Transcription factors that were originally identified as site-specific DNA-binding proteins essential for DNA REPLICATION by ADENOVIRUSES. They play important roles in MAMMARY GLAND function and development.
Experimentally induced new abnormal growth of TISSUES in animals to provide models for studying human neoplasms.
Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
Defective viruses which can multiply only by association with a helper virus which complements the defective gene. Satellite viruses may be associated with certain plant viruses, animal viruses, or bacteriophages. They differ from satellite RNA; (RNA, SATELLITE) in that satellite viruses encode their own coat protein.
The sum of the weight of all the atoms in a molecule.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
Nuclear phosphoprotein encoded by the p53 gene (GENES, P53) whose normal function is to control CELL PROLIFERATION and APOPTOSIS. A mutant or absent p53 protein has been found in LEUKEMIA; OSTEOSARCOMA; LUNG CANCER; and COLORECTAL CANCER.
The infective system of a virus, composed of the viral genome, a protein core, and a protein coat called a capsid, which may be naked or enclosed in a lipoprotein envelope called the peplos.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
Y-box-binding protein 1 was originally identified as a DNA-binding protein that interacts with Y-box PROMOTER REGIONS of MHC CLASS II GENES. It is a highly conserved transcription factor that regulates expression of a wide variety of GENES.
An alpha integrin with a molecular weight of 160-kDa that is found in a variety of cell types. It undergoes posttranslational cleavage into a heavy and a light chain that are connected by disulfide bonds. Integrin alphaV can combine with several different beta subunits to form heterodimers that generally bind to RGD sequence-containing extracellular matrix proteins.
Genes whose expression is easily detectable and therefore used to study promoter activity at many positions in a target genome. In recombinant DNA technology, these genes may be attached to a promoter region of interest.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Proteins which are involved in the phenomenon of light emission in living systems. Included are the "enzymatic" and "non-enzymatic" types of system with or without the presence of oxygen or co-factors.
Deoxycytidine (dihydrogen phosphate). A deoxycytosine nucleotide containing one phosphate group esterified to the deoxyribose moiety in the 2'-,3'- or 5- positions.
Enzymes that oxidize certain LUMINESCENT AGENTS to emit light (PHYSICAL LUMINESCENCE). The luciferases from different organisms have evolved differently so have different structures and substrates.
Method for measuring viral infectivity and multiplication in CULTURED CELLS. Clear lysed areas or plaques develop as the VIRAL PARTICLES are released from the infected cells during incubation. With some VIRUSES, the cells are killed by a cytopathic effect; with others, the infected cells are not killed but can be detected by their hemadsorptive ability. Sometimes the plaque cells contain VIRAL ANTIGENS which can be measured by IMMUNOFLUORESCENCE.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.
The binding of virus particles to receptors on the host cell surface. For enveloped viruses, the virion ligand is usually a surface glycoprotein as is the cellular receptor. For non-enveloped viruses, the virus CAPSID serves as the ligand.
Experimental transplantation of neoplasms in laboratory animals for research purposes.
A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.
Product of the retinoblastoma tumor suppressor gene. It is a nuclear phosphoprotein hypothesized to normally act as an inhibitor of cell proliferation. Rb protein is absent in retinoblastoma cell lines. It also has been shown to form complexes with the adenovirus E1A protein, the SV40 T antigen, and the human papilloma virus E7 protein.
The study of the structure, growth, function, genetics, and reproduction of viruses, and VIRUS DISEASES.
A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.
Vaccines or candidate vaccines containing inactivated HIV or some of its component antigens and designed to prevent or treat AIDS. Some vaccines containing antigens are recombinantly produced.
An ACYCLOVIR analog that is a potent inhibitor of the Herpesvirus family including cytomegalovirus. Ganciclovir is used to treat complications from AIDS-associated cytomegalovirus infections.
The relationships of groups of organisms as reflected by their genetic makeup.
Infections of the eye caused by minute intracellular agents. These infections may lead to severe inflammation in various parts of the eye - conjunctiva, iris, eyelids, etc. Several viruses have been identified as the causative agents. Among these are Herpesvirus, Adenovirus, Poxvirus, and Myxovirus.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
Processes that stimulate the GENETIC TRANSCRIPTION of a gene or set of genes.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
The rate dynamics in chemical or physical systems.
The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990)
Receptors such as INTEGRIN ALPHAVBETA3 that bind VITRONECTIN with high affinity and play a role in cell migration. They also bind FIBRINOGEN; VON WILLEBRAND FACTOR; osteopontin; and THROMBOSPONDINS.
A genus of ADENOVIRIDAE comprising species including viruses of frogs (FROGS AND TOADS) and TURKEYS. The type species is Frog adenovirus.
Directed modification of the gene complement of a living organism by such techniques as altering the DNA, substituting genetic material by means of a virus, transplanting whole nuclei, transplanting cell hybrids, etc.
The integration of exogenous DNA into the genome of an organism at sites where its expression can be suitably controlled. This integration occurs as a result of homologous recombination.
An enzyme that catalyzes the acetylation of chloramphenicol to yield chloramphenicol 3-acetate. Since chloramphenicol 3-acetate does not bind to bacterial ribosomes and is not an inhibitor of peptidyltransferase, the enzyme is responsible for the naturally occurring chloramphenicol resistance in bacteria. The enzyme, for which variants are known, is found in both gram-negative and gram-positive bacteria. EC 2.3.1.28.
Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
A malignant neoplasm made up of epithelial cells tending to infiltrate the surrounding tissues and give rise to metastases. It is a histological type of neoplasm but is often wrongly used as a synonym for "cancer." (From Dorland, 27th ed)
Any immunization following a primary immunization and involving exposure to the same or a closely related antigen.
Macromolecular molds for the synthesis of complementary macromolecules, as in DNA REPLICATION; GENETIC TRANSCRIPTION of DNA to RNA, and GENETIC TRANSLATION of RNA into POLYPEPTIDES.
A member of the p300-CBP transcription factors that was originally identified as a binding partner for ADENOVIRUS E1A PROTEINS.
Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.
A sequence of successive nucleotide triplets that are read as CODONS specifying AMINO ACIDS and begin with an INITIATOR CODON and end with a stop codon (CODON, TERMINATOR).
Polyomavirus antigens which cause infection and cellular transformation. The large T antigen is necessary for the initiation of viral DNA synthesis, repression of transcription of the early region and is responsible in conjunction with the middle T antigen for the transformation of primary cells. Small T antigen is necessary for the completion of the productive infection cycle.
CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). The species is a favorite for cytogenetic studies because of its small chromosome number. The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells.
Inactivation of viruses by non-immune related techniques. They include extremes of pH, HEAT treatment, ultraviolet radiation, IONIZING RADIATION; DESICCATION; ANTISEPTICS; DISINFECTANTS; organic solvents, and DETERGENTS.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.
A fractionated cell extract that maintains a biological function. A subcellular fraction isolated by ultracentrifugation or other separation techniques must first be isolated so that a process can be studied free from all of the complex side reactions that occur in a cell. The cell-free system is therefore widely used in cell biology. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p166)
A species of CERCOPITHECUS containing three subspecies: C. tantalus, C. pygerythrus, and C. sabeus. They are found in the forests and savannah of Africa. The African green monkey (C. pygerythrus) is the natural host of SIMIAN IMMUNODEFICIENCY VIRUS and is used in AIDS research.
Forceful administration into a muscle of liquid medication, nutrient, or other fluid through a hollow needle piercing the muscle and any tissue covering it.
Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.
A critical subpopulation of regulatory T-lymphocytes involved in MHC Class I-restricted interactions. They include both cytotoxic T-lymphocytes (T-LYMPHOCYTES, CYTOTOXIC) and CD8+ suppressor T-lymphocytes.
A genus of the family HERPESVIRIDAE, subfamily BETAHERPESVIRINAE, infecting the salivary glands, liver, spleen, lungs, eyes, and other organs, in which they produce characteristically enlarged cells with intranuclear inclusions. Infection with Cytomegalovirus is also seen as an opportunistic infection in AIDS.
The assembly of VIRAL STRUCTURAL PROTEINS and nucleic acid (VIRAL DNA or VIRAL RNA) to form a VIRUS PARTICLE.
New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms.
Proteins conjugated with deoxyribonucleic acids (DNA) or specific DNA.
Bacteriophage and type species in the genus Tectivirus, family TECTIVIRIDAE. They are specific for Gram-negative bacteria.
A genus of the family HERPESVIRIDAE, subfamily ALPHAHERPESVIRINAE, consisting of herpes simplex-like viruses. The type species is HERPESVIRUS 1, HUMAN.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
Antibodies that reduce or abolish some biological activity of a soluble antigen or infectious agent, usually a virus.
A species of ENTEROVIRUS infecting humans and containing 36 serotypes. It is comprised of all the echoviruses and a few coxsackieviruses, including all of those previously named coxsackievirus B.
Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).
The production of PEPTIDES or PROTEINS by the constituents of a living organism. The biosynthesis of proteins on RIBOSOMES following an RNA template is termed translation (TRANSLATION, GENETIC). There are other, non-ribosomal peptide biosynthesis (PEPTIDE BIOSYNTHESIS, NUCLEIC ACID-INDEPENDENT) mechanisms carried out by PEPTIDE SYNTHASES and PEPTIDYLTRANSFERASES. Further modifications of peptide chains yield functional peptide and protein molecules.
Proteins found mainly in icosahedral DNA and RNA viruses. They consist of proteins directly associated with the nucleic acid inside the NUCLEOCAPSID.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
The entering of cells by viruses following VIRUS ATTACHMENT. This is achieved by ENDOCYTOSIS, by direct MEMBRANE FUSION of the viral membrane with the CELL MEMBRANE, or by translocation of the whole virus across the cell membrane.
Vaccines or candidate vaccines used to prevent EBOLA HEMORRHAGIC FEVER.
Immunized T-lymphocytes which can directly destroy appropriate target cells. These cytotoxic lymphocytes may be generated in vitro in mixed lymphocyte cultures (MLC), in vivo during a graft-versus-host (GVH) reaction, or after immunization with an allograft, tumor cell or virally transformed or chemically modified target cell. The lytic phenomenon is sometimes referred to as cell-mediated lympholysis (CML). These CD8-positive cells are distinct from NATURAL KILLER CELLS and NATURAL KILLER T-CELLS. There are two effector phenotypes: TC1 and TC2.
Cis-acting DNA sequences which can increase transcription of genes. Enhancers can usually function in either orientation and at various distances from a promoter.
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
The top portion of the pharynx situated posterior to the nose and superior to the SOFT PALATE. The nasopharynx is the posterior extension of the nasal cavities and has a respiratory function.
Tumor suppressor genes located on the short arm of human chromosome 17 and coding for the phosphoprotein p53.
A conserved A-T rich sequence which is contained in promoters for RNA polymerase II. The segment is seven base pairs long and the nucleotides most commonly found are TATAAAA.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
A family of basic helix-loop-helix transcription factors that control expression of a variety of GENES involved in CELL CYCLE regulation. E2F transcription factors typically form heterodimeric complexes with TRANSCRIPTION FACTOR DP1 or transcription factor DP2, and they have N-terminal DNA binding and dimerization domains. E2F transcription factors can act as mediators of transcriptional repression or transcriptional activation.

CAR-dependent and CAR-independent pathways of adenovirus vector-mediated gene transfer and expression in human fibroblasts. (1/2914)

Primary fibroblasts are not efficiently transduced by subgroup C adenovirus (Ad) vectors because they express low levels of the high-affinity Coxsackie virus and adenovirus receptor (CAR). In the present study, we have used primary human dermal fibroblasts as a model to explore strategies by which Ad vectors can be designed to enter cells deficient in CAR. Using an Ad vector expressing the human CAR cDNA (AdCAR) at high multiplicity of infection, primary fibroblasts were converted from being CAR deficient to CAR sufficient. Efficiency of subsequent gene transfer by standard Ad5-based vectors and Ad5-based vectors with alterations in penton and fiber was evaluated. Marked enhancement of binding and transgene expression by standard Ad5 vectors was achieved in CAR-sufficient fibroblasts. Expression by AdDeltaRGDbetagal, an Ad5-based vector lacking the arginine-glycine-aspartate (RGD) alphaV integrin recognition site from its penton base, was achieved in CAR-sufficient, but not CAR-deficient, cells. Fiber-altered Ad5-based vectors, including (a) AdF(pK7)betagal (bearing seven lysines on the end of fiber) (b) AdF(RGD)betagal (bearing a high-affinity RGD sequence on the end of fiber), and (c) AdF9sK betagal (bearing a short fiber and Ad9 knob), demonstrated enhanced gene transfer in CAR-deficient fibroblasts, with no further enhancement in CAR-sufficient fibroblasts. Together, these observations demonstrate that CAR deficiency on Ad targets can be circumvented either by supplying CAR or by modifying the Ad fiber to bind to other cell-surface receptors.  (+info)

Reduced phosphorylation of p50 is responsible for diminished NF-kappaB binding to the major histocompatibility complex class I enhancer in adenovirus type 12-transformed cells. (2/2914)

Reduced cell surface levels of major histocompatibility complex class I antigens enable adenovirus type 12 (Ad12)-transformed cells to escape immunosurveillance by cytotoxic T lymphocytes (CTL), contributing to their tumorigenic potential. In contrast, nontumorigenic Ad5-transformed cells harbor significant cell surface levels of class I antigens and are susceptible to CTL lysis. Ad12 E1A mediates down-regulation of class I transcription by increasing COUP-TF repressor binding and decreasing NF-kappaB activator binding to the class I enhancer. The mechanism underlying the decreased binding of nuclear NF-kappaB in Ad12-transformed cells was investigated. Electrophoretic mobility shift assay analysis of hybrid NF-kappaB dimers reconstituted from denatured and renatured p50 and p65 subunits from Ad12- and Ad5-transformed cell nuclear extracts demonstrated that p50, and not p65, is responsible for the decreased ability of NF-kappaB to bind to DNA in Ad12-transformed cells. Hypophosphorylation of p50 was found to correlate with restricted binding of NF-kappaB to DNA in Ad12-transformed cells. The importance of phosphorylation of p50 for NF-kappaB binding was further demonstrated by showing that an NF-kappaB dimer composed of p65 and alkaline phosphatase-treated p50 from Ad5-transformed cell nuclear extracts could not bind to DNA. These results suggest that phosphorylation of p50 is a key step in the nuclear regulation of NF-kappaB in adenovirus-transformed cells.  (+info)

Microtubule-dependent plus- and minus end-directed motilities are competing processes for nuclear targeting of adenovirus. (3/2914)

Adenovirus (Ad) enters target cells by receptor-mediated endocytosis, escapes to the cytosol, and then delivers its DNA genome into the nucleus. Here we analyzed the trafficking of fluorophore-tagged viruses in HeLa and TC7 cells by time-lapse microscopy. Our results show that native or taxol-stabilized microtubules (MTs) support alternating minus- and plus end-directed movements of cytosolic virus with elementary speeds up to 2.6 micrometer/s. No directed movement was observed in nocodazole-treated cells. Switching between plus- and minus end-directed elementary speeds at frequencies up to 1 Hz was observed in the periphery and near the MT organizing center (MTOC) after recovery from nocodazole treatment. MT-dependent motilities allowed virus accumulation near the MTOC at population speeds of 1-10 micrometer/min, depending on the cell type. Overexpression of p50/dynamitin, which is known to affect dynein-dependent minus end-directed vesicular transport, significantly reduced the extent and the frequency of minus end-directed migration of cytosolic virus, and increased the frequency, but not the extent of plus end-directed motility. The data imply that a single cytosolic Ad particle engages with two types of MT-dependent motor activities, the minus end- directed cytoplasmic dynein and an unknown plus end- directed activity.  (+info)

Differences in the interactions of oncogenic adenovirus 12 early region 1A and nononcogenic adenovirus 2 early region 1A with the cellular coactivators p300 and CBP. (4/2914)

Association with the cellular coactivators p300 and CBP is required for the growth-regulatory function of adenoviral (Ad) early region 1A (E1A) proteins. E1A regions necessary for these interactions overlap with domains involved in the induction of tumours in immunocompetent rodents through highly oncogenic Ad12. Differences in the association of cellular factors with the respective E1A domains of Ad12 and nononcogenic Ad2 might therefore be involved in serotype-specific oncogenicity. We analyzed the interaction of the Ad12 E1A 235R protein with p300 and CBP. Here we demonstrate that in the case of Ad12, but not Ad2/5, amino acids (aa) 1-29 of E1A proteins are sufficient to bind the p300-C/H3 domain in vivo and wild-type p300 in vitro. The conserved arginine-2, which is essential for the interaction between Ad2 E1A and p300, was dispensable for the Ad12 E1A 235R-p300 interaction in vitro. In addition to the p300-C/H3 region, we identified a second domain within p300 (aa 1999-2200) binding to the 235R protein. Contrary to p300, the amino-terminus and CR1 are necessary to associate with CBP. The aa 1-29 of the 235R protein but not CR1 are essential for the repression of colTRE-driven gene expression. This repression function is strictly dependent on p300 but not on CBP.  (+info)

Evidence for an adenovirus type 2-coded early glycoprotein. (5/2914)

We have identified an adenovirus type 2 (Ad2)-induced early glycopolypeptide with an apparent molecular weight of 20,000 to 21,000 (20/21K), as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The 20/21K polypeptide could be labeled in vivo with [(3)H]glucosamine. [(35)S]methionine- and [(3)H]-glucosamine-labeled 20/21K polypeptides bound to concanavalin A-Sepharose columns and were eluted with 0.2 M methyl-alpha-d-mannoside. The pulse-labeled polypeptide appeared as a sharp band with an apparent molecular weight of 21K, but after a chase it converted to multiple bands with an average molecular weight of 20K. This variability in electrophoretic mobility is consistent with glycosylation or deglycosylation of the 20/21K polypeptide. Analysis of the pulse and pulse-chase-labeled forms by using partial proteolysis indicated that the polypeptides were highly related chemically, but not identical. Most of the 20/21K polypeptide is localized in the cytoplasm fraction of infected cells lysed by Nonidet P-40. The 20/21K polypeptide and a 44K polypeptide, labeled with [(35)S]methionine or [(3)H]glucosamine in Ad2-infected human cells, were precipitated by a rat antiserum against an Ad2-transformed rat cell line (T2C4), but not by antisera against three other Ad2-transformed rat cell lines, or by serum from nonimmune rats. The partial proteolysis patterns of the 20/21K and the 44K polypeptides were indistinguishable, indicating that the two polypeptides are highly related, and suggesting that the 44K polypeptide might be a dimer of the 20/21K polypeptide. The 20/21K polypeptide was also induced in Ad2-early infected monkey and hamster cells. These results imply that the 20/21K polypeptide is synthesized in Ad2-infected human, monkey, and hamster cells, and in one but not all Ad2-transformed rat cells. Thus, the 20/21K polypeptide is probably viral coded rather than cell coded and viral induced.  (+info)

Development and use of a 293 cell line expressing lac repressor for the rescue of recombinant adenoviruses expressing high levels of rabies virus glycoprotein. (6/2914)

An expression cassette designed for high-level production of rabies virus glycoprotein (RG) could not be rescued into a replication-defective, adenovirus-based vector using standard procedures. To overcome this difficulty, a 293-based cell line, designated 293LAP13, was constructed that contained and expressed a derivative of the lac repressor protein. The lac operator sequence, to which the repressor binds, was incorporated into an expression cassette, containing a promoter and intron, designed for high-level production of RG. Insertion of a single operator sequence immediately downstream of the transcription start site and the use of the 293LAP13 cell line allowed recombinant viruses that could not be isolated with 293 cells to be rescued efficiently. The operator-containing virus reached higher titres in 293LAP13 than in parental 293 cells and also produced plaques more efficiently in 293LAP13 cells. Moreover, in non-complementing human and canine cell lines, adenovirus vectors with a promoter-intron expression cassette expressed RG at much higher levels than vectors lacking the intron. These observations, together with the demonstration that expression of RG by operator-containing vectors was repressed markedly in 293LAP13 cells and that this inhibition was relieved at least partly by IPTG, suggest that the 293LAP13 cell line may be useful for the rescue and propagation of many vectors in which high expression of the desired protein prevents vector rescue in 293 cells.  (+info)

The adenoviral E1A oncoproteins interfere with the growth-inhibiting effect of the cdk-inhibitor p21(CIP1/WAF1). (7/2914)

The cdk-inhibitor p21(CIP1/WAF1) inhibits the activities of cyclin-dependent kinases and proliferating cell nuclear antigen, thereby repressing cell-cycle progression and DNA replication. Transforming oncogenes, such as E1A of human adenovirus 5 (Ad5), may interfere with such growth-inhibitory proteins. In this study, we show that in various Ad5E1-transformed cells, p21(CIP1/WAF1) is expressed and that, in general, expression is not downregulated. In addition, colony-formation assays show that in Ad5E1-transformed cells highly overexpressed p21(CIP1/WAF1) can still cause growth inhibition. FACS experiments indicate, however, that a G1 arrest induced by moderate overexpression of p21(CIP1/WAF1) can be overcome by E1A. The E1A proteins may interfere with the function of p21(CIP1/WAF1) by binding. Indeed, p21(CIP1/WAF1) binds with its cyclin/cdk-binding N terminus to the transforming N-terminal and CR1 region of the E1A proteins. Together, these results lend support to the model that E1A can interfere directly with p21(CIP1/WAF1) function and thereby stimulates cell growth.  (+info)

Early region 1 transforming functions are dispensable for mammary tumorigenesis by human adenovirus type 9. (8/2914)

Some human adenoviruses are tumorigenic in rodents. Subgroup A and B human adenoviruses generally induce sarcomas in both male and female animals, and the gene products encoded within viral early region 1 (E1 region) are both necessary and sufficient for this tumorigenicity. In contrast, subgroup D human adenovirus type 9 (Ad9) induces estrogen-dependent mammary tumors in female rats and requires the E4 region-encoded ORF1 oncoprotein for its tumorigenicity. Considering the established importance of the viral E1 region for tumorigenesis by adenoviruses, we investigated whether this viral transcription unit is also necessary for Ad9 to generate mammary tumors. The nucleotide sequence of the Ad9 E1 region indicated that the gene organization and predicted E1A and E1B polypeptides of Ad9 are closely related to those of other human adenovirus E1 regions. In addition, an Ad9 E1 region plasmid demonstrated focus-forming activity in both low-passage-number and established rat embryo fibroblasts, whereas a large deletion within either the E1A or E1B gene of this plasmid diminished transforming activity. Surprisingly, we found that introducing the same transformation-inactivating E1A and E1B deletions into Ad9 results in mutant viruses that retain the ability to elicit mammary tumors in rats. These results are novel in showing that Ad9 represents a unique oncogenic adenovirus in which the E4 region, rather than the E1 region, encodes the major oncogenic determinant in the rat.  (+info)

Transarterial chemoembolization (TACE) is currently one of the mainstays of palliative treatments worldwide for patients with unresectable Hepatocellular Carcinoma(HCC).However, the long term outcomes were generally poor for HCC patients treated with TACE. Recombinant Human Adenovirus Type 5, an E1B gene deleted adenovirus, is known to have a significant antitumor activity. In addition, local injection of recombinant human adenovirus type 5 can enhance the effect of antitumor therapies (chemotherapy and radiotherapy). The hypothesis is that patients with unresectable HCC may benefit from recombinant human adenovirus type 5 in combination with TACE ...
Summary Evidence is presented here which indicates that the adenovirus DNA-binding protein (DBP) is phosphorylated at a tyrosine residue early in infection. This was suggested by the discovery that a proportion of the label in 32P-labelled DBP was resistant to alkali, and was substantiated by acid hydrolysis of DBP immunoprecipitates and by immunoblotting with a monoclonal antibody against phosphotyrosine. Treatment of [35S] methionine-labelled DBPs with chymotrypsin produced fragments of apparent M r 45K and 39K whereas digestion of 32P-labelled DBP resulted in fragments of 45K and 26K. Consideration of the distribution of 32P label and its alkali stability in these fragments suggested that chymotrypsin cleaved populations of DBP at different sites depending on their phosphorylation states. The conservation, in all of the seven adenovirus serotypes sequenced, of a tyrosine residue (at amino acid 195 in adenovirus type 2) together with its surrounding residues, suggests that phosphorylation
Human adenoviruses are non-enveloped dsDNA viruses of almost 35 kb in size [1]. HAdV can infect a variety of tissues and cause a wide range of complications like gastroenteritis, hepatitis, myocarditis, keratoconjunctivitis and pneumonia [2, 3]. It is contagion in nature which occurs through direct contact or fomites and virus is also resistant to various physical and chemical agents. Children younger than the age of 5 years and immune compromised persons especially the pediatric patients are most susceptible to these viruses. Worldwide 5-7% respiratory tract infections are ascribed by HAdV in pediatric patients [4] and persons of all ages are susceptible to infections caused by these viruses [5].. Seven known Human adenoviruses species from HAdV-A to HAdV-G are constitute of the genus Mastadenovirus in which all the human adenoviruses are categorized and further divided into different strains [6]. Now 67 types of HAdV have been reported [7]. Their number is rapidly increasing due to ...
Fingerprint Dive into the research topics of Accumulation of early and intermediate mRNA species during subgroup C adenovirus productive infections. Together they form a unique fingerprint. ...
In 2005, a human adenovirus strain (formerly known as HAdV-D22/H8 but renamed here HAdV-D53) was isolated from an outbreak of epidemic keratoconjunctititis (EKC), a disease that is usually caused by HAdV-D8, -D19, or -D37, not HAdV-D22. To date, a complete change of tropism compared to the prototype has never been observed, although apparent recombinant strains of other viruses from species Human adenovirus D (HAdV-D) have been described. The complete genome of HAdV-D53 was sequenced to elucidate recombination events that lead to the emergence of a viable and highly virulent virus with a modified tropism. Bioinformatic and phylogenetic analyses of this genome demonstrate that this adenovirus is a recombinant of HAdV-D8 (including the fiber gene encoding the primary cellular receptor binding site), HAdV-D22, (the ε determinant of the hexon gene), HAdV-D37 (including the penton base gene encoding the secondary cellular receptor binding site), and at least one unknown or unsequenced HAdV-D strain.
In 2005, a human adenovirus strain (formerly known as HAdV-D22/H8 but renamed here HAdV-D53) was isolated from an outbreak of epidemic keratoconjunctititis (EKC), a disease that is usually caused by HAdV-D8, -D19, or -D37, not HAdV-D22. To date, a complete change of tropism compared to the prototype has never been observed, although apparent recombinant strains of other viruses from species Human adenovirus D (HAdV-D) have been described. The complete genome of HAdV-D53 was sequenced to elucidate recombination events that lead to the emergence of a viable and highly virulent virus with a modified tropism. Bioinformatic and phylogenetic analyses of this genome demonstrate that this adenovirus is a recombinant of HAdV-D8 (including the fiber gene encoding the primary cellular receptor binding site), HAdV-D22, (the ε determinant of the hexon gene), HAdV-D37 (including the penton base gene encoding the secondary cellular receptor binding site), and at least one unknown or unsequenced HAdV-D strain.
Entry of adenoviruses into the host cell involves two sets of interactions between the virus and the host cell. Most of the action occurs at the vertices. Entry into the host cell is initiated by the knob domain of the fiber protein binding to the cell receptor. The two currently established receptors are: CD46 for the group B human adenovirus serotypes and the coxsackievirus adenovirus receptor (CAR) for all other serotypes. There are some reports suggesting MHC molecules and sialic acid residues functioning in this capacity as well. This is followed by a secondary interaction, where a motif in the penton base protein interacts with an integrin molecule. It is the co-receptor interaction that stimulates entry of the adenovirus. This co-receptor molecule is αv integrin. Binding to αv integrin results in endocytosis of the virus particle via clathrin-coated pits. Attachment to αv integrin stimulates cell signaling and thus induces actin polymerization resulting in entry of the virion into the ...
Adenovirus genomes are linear, non-segmented double-stranded (ds) DNA molecules that are typically 26-46 Kbp long, containing 23-46 protein-coding genes. The example used for the following description is Human adenovirus E, a mastadenovirus with a 36 Kbp genome containing 38 protein-coding genes. While the precise number and identity of genes varies among adenoviruses, the basic principles of genome organization and the functions of most of the genes described in this article are shared among all adenoviruses. The 38 genes in the Human Adenovirus E genome are organized in 17 transcription units, each containing 1-8 coding sequences. Alternative splicing during processing of the pre-mRNAs produced by each transcription unit enable multiple different mRNAs to be produced from one transcription unit. The E1A, E1B, E2A, E2B, E3, and E4 transcription units are successively transcribed early in the viral reproductive cycle. The proteins coded for by genes within these transcription units are mostly ...
To define the bottlenecks that restrict antigen expression after oral administration of viral-vectored vaccines, we tracked vectors derived from the human adenovirus type 5 at whole body, tissue and cellular scales throughout the digestive tract in a murine model of oral delivery. After intragastric administration of vectors encoding firefly luciferase or a model antigen, detectable levels of transgene-encoded protein or mRNA were confined to the intestine, and restricted to delimited anatomical zones. Expression of luciferase in the form of multiple small bioluminescent foci in the distal ileum, cecum and proximal colon suggested multiple crossing points. Many foci were unassociated with visible Peyers patches, implying that transduced cells lay in proximity to villous rather than follicle-associated epithelium, as supported by detection of transgene-encoded antigen in villous epithelial cells. Transgene-encoded mRNA but not protein was readily detected in Peyers patches, suggesting that post
Human adenovirus (HAdV) is a common pathogen causing a broad spectrum of diseases. HAdV encodes the pVII protein, which is involved in nuclear delivery, protection and expression of viral DNA. To suppress the cellular interferon (IFN) and RNA interference (RNAi) systems, HAdVs encode non-coding virus-associated (VA) RNAs. In this thesis we have investigated the functional significance of the pVII protein and VA RNAI in HAdV-5 infected cells.. We report that the propeptide module is the destabilizing element targeting the precursor pVII protein for proteasomal degradation. We also found that the Cul3-based E3 ubiquitin ligase complex alter the precursor pVII protein stability via binding to the propeptide sequence. In addition, we show that inhibition of the Cul3 protein reduces HAdV-5 E1A gene expression. Collectively, our results suggest a novel function for the pVII propeptide module and involvement of Cul3 in viral E1A gene expression.. Our studies show that the cellular E3 ubiquitin ligase ...
Human adenovirus 4 ATCC ® VR-1572D™ Designation: DNA from Human adenovirus 4 strain RI-67 [ATCC ® VR-1572™] Application: It is suitable for use in polymerase chain reaction (PCR) for viral gene products and other molecular virology applications. Respiratory research
Human adenovirus 12 ATCC ® VR-863D™ Designation: DNA from Human adenovirus 12 strain Huie [ATCC ® VR-863™] Application: It is suitable for use in polymerase chain reaction (PCR) for viral gene products and other molecular virology applications. Respiratory research
It has been known for some time that the human adenovirus serotype 5 (Ad5) E4orf6 and Sox18 E1B55K proteins work in concert to degrade p53 and to regulate selective export of late viral mRNAs during productive infection. H1299 human lung carcinoma cells after expression of E1B55K and E4orf6 using adenovirus vectors. Several species were detected and identified by mass spectroscopy and for one of these integrin α3 we went on in a parallel study to confirm it as a substrate of the complex (F. Dallaire et al. J. Virol. 83:5329-5338 2009 Although the system has some limitations it may still be of some general use in identifying candidate substrates of any viral cullin-based E3 ubiquitin ligase complex and we suggest a series of criteria for substrate validation. During the past decade protein degradation has become increasingly recognized SVT-40776 (Tarafenacin) as a critical mechanism by which cells regulate a number of fundamental processes (reviewed in references 37 57 and 59). Degradation ...
Adenoviral nucleic acid was detected by polymerase chain reaction (PCR) in pharyngeal and rectal swab samples of a cat seropositive for adenovirus and suffering from transient hepatic failure. The samples were taken at a one-year interval, and both faecal samples as well as the second pharyngeal sample were positive in ... read more PCR performed with general adenovirus primers. The size of the amplified products corresponded to that of the positive control. The identity of the amplicons was also confirmed by DNA sequencing. The 301 bp long hexon gene fragment was very similar to but distinguishable from the corresponding hexon sequence of human adenovirus type 2. This result suggests the possibility of persistent carrier status and shedding of adenovirus in cats. show less ...
Antisera against hexons of serotypes 2, 4, 5, and 6 (subgroup III), and 15 (subgroup II) were absorbed with purified hexons of various serotypes representing the different subgroups of human adenoviruses. Group, subgroup, and type specificities of hexons could be distinguished. The subgroup specificity of type 4 hexons resembled that of hexons of subgroup I members (types 3, 11, and 16). Antihexon sera gave a type-specific inhibition of virion-associated hemagglutinin. The inhibiting activity of different sera was found to be inversely related to the length of fibers of the serotype concerned. Virions of serotypes carrying fibers shorter than about 20 nm (types 3, 4, 9, 11, and 15) were readily inhibited, whereas those of serotypes with longer fibers (types 2 and 6) were inhibited only by relatively large amounts of antibody measured in terms of homotypic complement fixation activity. The reciprocal cross-neutralization between serotypes 4 and 16 was studied separately. Hexons of both serotypes ...
The precursor of the 55K adenovirus terminal protein is an 87K protein that is covalently linked to viral DNA. This protein is likely to be identical to the 80,000 dalton protein described by Challberg et al. (1980). The mRNA for the 87K terminal protein precursor, like that for the E2-72K DNA binding protein, is detectable at both early and late times of infection, and its production is sensitive to protein synthesis inhibition (Lewis and Mathews, 1980). The 87K protein, together with proteins of 105,000 and 75,000 daltons, are translated from leftward transcribed (1-strand) messenger RNAs that are complementary to the viral genome between positions 11.2 and 31.5. Additional hybridization to the region between coordinates 37.3 and 41 suggests that the RNA body is spliced to sequences mapping farther right in the genome. Electron microscopic heteroduplex analysis has revealed a family of 1-strand RNAs that probably encode these proteins. The RNA bodies extend from coordinated 30, 26 and 23 to ...
Recombinant adenoviruses currently are used for a variety of purposes, including gene transfer in vitro, vaccination in vivo, and gene therapy (1-4). Several features of adenovirus biology have made such viruses the vectors of choice for certain of these applications. For example, adenoviruses transfer genes to a broad spectrum of cell types, and gene transfer is not dependent on active cell division. Additionally, high titers of viruses and high levels of transgene expression generally can be obtained.. Decades of study of adenovirus biology have resulted in a detailed picture of the viral life cycle and the functions of the majority of viral proteins (5, 6). The genome of the most commonly used human adenovirus (serotype 5) consists of a linear, 36-kb, double-stranded DNA molecule. Both strands are transcribed and nearly all transcripts are heavily spliced. Viral transcription units are conventionally referred to as early (E1, E2, E3, and E4) and late, depending on their temporal expression ...
The cryo-electron microscopy (cryo-EM) structure of the complex between the trimeric human adenovirus B serotype 3 fibre knob and human desmoglein 2 fragments containing cadherin domains EC2 and EC3 has been published, showing 3:1 and 3:2 complexes. Here, the crystal structure determined at 4.5 Å resolution is presented with one EC2-EC3 desmoglein fragment bound per fibre knob monomer in...
Adenovirus has been associated with both sporadic and epidemic disease and, with regard to infections among military recruits, who were routinely immunized against types 4 and 7 from 1971 until the cessation of vaccine production in 1996. Adenovirus became a significant cause of economic cost and morbidity in this setting. A live oral vaccine against adenovirus types 4 and 7 was approved for use in this population by the US Food and Drug Administration (FDA) in 2011, and subsequent incidence of acute respiratory disease declined.. Of interest is the role of adenoviruses as vectors in vaccination and in gene therapy. [1, 2, 3] Adenoviruses can infect various cells, both proliferating and quiescent, and thus hold the promise of targeting many different tissues and diseased cell lines.. The genome of adenovirus is well known and can be modified with relative ease to induce lysis or cytotoxicity of a specified cell line without affecting others.. The virus itself can be engineered to remove its ...
The E4 region of human adenovirus type 2 is predicted to encode seven proteins as judged from its nucleotide sequence and the pattern of differential splicing of its transcript. Two of the open reading frames (ORFs), ORF1 and ORF2, had been identified as being disrupted in the recently published sequence of the related serotype 5 virus. These ORFs were resequenced and found to be intact in the wt300 strain of adenovirus type 5.
Most adenoviruses bind directly to the coxsackie and adenovirus receptor (CAR) on target cells in vitro, but recent research has shown that adenoviruses can also use soluble components in body fluids for indirect binding to target cells. These mechanisms have been identified upon addressing the questions of how to de- and retarget adenovirus-based vectors for human gene and cancer therapy, but the newly identified mechanisms also suggest that the role of body fluids and their components may also be of importance for natural, primary infections. Here we demonstrate that plasma, saliva, and tear fluid promote binding and infection of adenovirus type 5 (Ad5) in respiratory and ocular epithelial cells, which corresponds to the natural tropism of most adenoviruses, and that plasma promotes infection by Ad31. By using a set of binding and infection experiments, we also found that Ad5 and Ad31 require coagulation factors IX (FIX) or X (FX) or just FIX, respectively, for efficient binding and infection. ...
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The quest for an efficacious HIV vaccine has resulted in several clinical trial failures, including the Step Trial, which used a replication-incompetent adenovirus (AdV) vector called human adenovirus type 5 (HAdV-5). Despite eliciting strong cellular immune responses, these trials were prematurely halted due to statistical futility resulting from increased HIV acquisition in vaccinated individuals. The Step Study showed increased HIV susceptibility in HAdV-5 baseline seropositive subjects, which complicates the use of HAdV-5 vectors since pre-existing neutralizing antibodies (nAb) to HAdV-5 are prevalent worldwide. Sampling the unique immunological phenotypes of the rectal mucosa - the site of HIV infection in the Step Study, and of AdV persistence and trafficking - could help explain this trial, since only peripheral blood (PBMC) was collected from subjects. We obtained rectal lamina propria T lymphocytes (rLPL) from a rhesus macaque (RM) model vaccinated with a species-specific simian Ad type 7 (SAdV
TY - JOUR. T1 - Repression of cytochrome P‐450c gene expression by cotransfection with adenovirus E1a DNA. AU - SOGAWA, Kazuhiro. AU - HANDA, Hiroshi. AU - FUJISAWA‐SEHARA, Atsuko. AU - HIROMASA, Takako. AU - YAMANE, Miyuki. AU - FUJII‐KURIYAMA, Yoshiaki. PY - 1989/5. Y1 - 1989/5. N2 - Gene expression of rat cytochrome P‐450c (P‐450c) depends upon inducible enhancers scattered in the 5′‐upstream region of the gene. We show that expression of the P‐450c gene is repressed by contransfection with adenovirus E1a DNA, regardless of the presence or absence of inducers, in a transient expression system of HeLa cells. Since cotransfection of either 13S or 12S E1a cDNA was effective in the repression, the region necessary for repression could be separated from that of transactivation of other adenovirus early genes. Moreover, we investigated the regions responsible for the inhibitory activity using in‐frame deletion mutants lacking internal or external portions of the E1a proteins. The ...
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SignaGen Laboratories, A Gene Delivery Company Providing Custom AAV Adenovirus Lentivirus Production Services & Manufacturing DNA/siRNA Transfection Reagents... Ad-GIT2 [SL100903] - Product Category: Human Adenovirus Type5 (dE1/E3) expressing G Protein-coupled Receptor Kinase Interactor 2 (GIT2) under a CMV promoter. Product Information Product Name: G Protein-coupled Receptor Kinase Interactor 2, pre-packaged adenovirus, ready to ship and ready to use format. Promoter: CMV Titer: 1E+10~1E+11 PFU/ml Storage Buffer: DMEM with 2.5% BSA, 2.5% glycerol Gene Information Gene Name: G
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
There are many stages in the development of a new drug for viral infection and such processes are even further complicated for adenovirus by the fact that there are at least 51 serotypes, forming six distinct groups (A-F), with different degree of infectivity. This review attempts to address the importance of developing pharmaceuticals for adenovirus and also review recent development in drug discovery for adenovirus, including newer strategies such as microRNA approaches. Different drug screening strategies will also be discussed.
Adenovirus Type 9, 0.1 mg. The many different serotypes of human adenoviruses (Ad) are divided into six subgroups, of which all Ad subgroup A and B and two subgroup D Ads can elicit tumors in infected rodents.
A HIV-1 DNA prime-recombinant Adenovirus Type 5 (rAd5) increase vaccine failed to guard against HIV-1 acquisition. is certainly towards the gp41 subunit from the envelope (Env) glycoprotein from the pathogen (1). This antibody response derives from polyreactive B cells that cross-react with Env and intestinal microbiota (IM) (2, 3). Nevertheless, it is unidentified if an identical gp41-reactive Ab response would take place in the placing of HIV-1 Env vaccination. A DNA leading, recombinant adenovirus serotype 5 (rAd5) increase vaccine that included HIV and genes, and a trivalent combination of clade A, B and C gp140 genes formulated with both gp120 and gp41 elements was examined in the HIV Vaccine Studies Network (HVTN) [stage Ib (HVTN 082), GW-786034 stage II (HVTN 204), stage IIb (HVTN 505) efficiency trial] and various other clinical studies [stage I/II (RV172), stage I (V001)] (4C7). This vaccine was the GW-786034 initial vaccine formulated with the ectodomain from the Env gp41 component, ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
In tumor cells, the p53 pathway is often disrupted. Therefore, recovering the function of wild-type p53 and its targets in tumor cells is a key therapeutic objective. In head and neck cancer, p53 mutations are frequent, and the incidence of p53 mutations increases with progression of head and neck cancer (46, 47). Therefore, a recombinant human adenovirus that expresses functional wild-type p53 has been approved by the Chinese government for the treatment of head and neck carcinoma (48-50). Treatments showed that antitumor efficacy was associated with the expression and activity of functional p53, and adverse effects were also significant (51-54). Recently, pharmacologically activated wild-type p53 by small-molecule compound RITA is reported to inhibit glycolytic enzymes and, therefore, induce robust apoptosis in cancer cells (55). In addition, enhancement of p53 protein stability is also a target in restoring wild-type p53 activity in cancer cells. The protein level of wild-type p53 is ...
Mouse anti Adenovirus Hexon antibody, clone 7C11 reacts with human, canine, bovine, monkey and rat adenoviruses. It is very likely that it
13:2; potential epub Adenovirus notions; tenure bhakti 1? On the epub Adenovirus Methods and of this interest, are above under form. 1282 An epub Adenovirus Methods and Protocols: Adenoviruses, Ad study read by Porten - Szubin 1987:187.
Greetings- Im interested in the net surface charge on mammalian viruses and how viral charge affects viral adsorption. It is my understanding (correct me if Im wrong) that mammalian viruses have a net negative charge at physiologic pH. I find this interesting because of the net negative charge on mammalian cells due to their phospholipid content. I have been working with human adenovirus gene transfer vectors and have found that the pI of adeno capsid proteins are: Hexon = 4.5 and penton = 4.7. pI of fiber protein on adenovirus is reported to be 7.07. From these data, I have concluded that, at pH 7.0, an adeno virus particle has a negative core (Hexon and penton) with positively charged fiber protrusions. Is this correct (my chemistry is a little rusty)? If so, it would explain how negatively charged adenovirus particles might interact with negatively charged cell surfaces (via positive fiber protein). Thank you, in advance for any comments and corrections. In reading this note, if any related ...
1NLN: Crystallographic structure at 1.6-A resolution of the human adenovirus proteinase in a covalent complex with its 11-amino-acid peptide cofactor: insights on a new fold
The adenovirus E1B gene products are required for productive infection of human cells and for complete transformation of rodent cells in cooperation with the E1A gene products. Two major, unrelated polypeptides of 55,000 (55K) and 19,000 (19K) daltons are encoded by the E1B region. The 55K protein is required for efficient DNA replication, late mRNA transport to the cytoplasm and shut-off of cellular mRNA transport in productively infected cells. This protein is required for virus-mediated, but not DNA-mediated, transformation of rodent cells. It appears that the 55K protein does not directly contribute to cell transformation, but influences the oncogenicity of adenoviruses when they are inoculated into newborn hamsters. In contrast, the 19K protein is required for adenovirus induced cellular transformation and oncogenicity and localizes to membranes of the nuclear envelope, cytoplasm and the cell surface in transformed cells. This protein affects the efficiency of virus growth in some, but not ...
TY - JOUR. T1 - Conserved region 2 of adenovirus E1A has a function distinct from pRb binding required to prevent cell cycle arrest by p16(INK4a) or p27(Kip1). AU - Alevizopoulos, Konstantinos. AU - Sanchez, Belén. AU - Amati, Bruno. PY - 2000/4/13. Y1 - 2000/4/13. N2 - Ectopic expression of the CDK inhibitors (CKIs) p16(INK4a) and p27(Kip1) in Rat1 fibroblasts induces dephosphorylation and activation of Retinoblastoma-family proteins (pRb, p107 and p130), their association with E2F proteins, and cell cycle arrest in G1. The growth-inhibitory action of p16, in particular, is believed to be mediated essentially via pRb activation. The 12S E1A protein of human Adenovirus 5 associates with pRb-family proteins via residues in its Conserved Regions (CR) 1 and 2, in particular through the motif LXCXE in CR2. These interactions are required for E1A to prevent G1 arrest upon co-expression of CKIs. We show here that mutating either of two conserved motifs adjacent to LXCXE in CR2, GFP and SDDEDEE, also ...
Much concern has focused on the direct toxic effects of adenoviruses, particularly as intravenous administration of the virus can induce acute liver injury, as shown in animal models. It is this effect which may have triggered the cascade of events leading to the death of the patient with OTC deficiency-in this case the recombinant virus was injected directly into the hepatic artery. Studies in mice have highlighted the dose limiting liver toxicity of intravenously administered virus, which in this model is mainly due to an acute inflammatory response involving the release of certain cytokines (interleukin 6, interleukin 8, tumour necrosis factor α) and the recruitment of immune effector cells into the liver.5-7These effects are manifest within the first few hours of adenovirus administration and do not require de novo virus gene expression. A recent study demonstrated that adenovirus induced chemokine gene expression within the liver occurs within one hour after infection and results in the ...
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Fingerprint Dive into the research topics of Two early mRNA species in adenovirus type 2 transformed rat cells. Together they form a unique fingerprint. ...
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Looking for online definition of adenovirus early region genes in the Medical Dictionary? adenovirus early region genes explanation free. What is adenovirus early region genes? Meaning of adenovirus early region genes medical term. What does adenovirus early region genes mean?
RIGOTTO, C et al. Evaluation of HA negatively charged membranes in the recovery of human adenoviruses and hepatitis A virus in different water matrices. Mem. Inst. Oswaldo Cruz [online]. 2009, vol.104, n.7, pp.970-974. ISSN 0074-0276. http://dx.doi.org/10.1590/S0074-02762009000700005.. Human adenoviruses (HAdV) and hepatitis A virus (HAV) are shed in the faeces and consequently may be present in environmental waters, resulting in an increase in pathogen concentration that can affect water quality and human health. The aim of this study was to evaluate an adsorption-elution method which utilizes negatively charged membrane HA to determine the efficient recovery of HAdV and HAV from different water matrices and to combine this procedure with a qualitative molecular method (nested RT-PCR and nested PCR). The best efficiency recovery was achieved in distilled water and treated wastewater effluent (100%) for both viruses and in recreational lagoon water for HAV (100%). The efficiency recovery was 10% ...
Human adenovirus, of the Adenoviridae family, is a nonenveloped icosahedral particle containing a single linear dsDNA genome. Human adenovirus comprises 6 species (A through F) consisting of serotypes 1 - 51, and associated with a variety of clinical illnesses. Serotypes 1-39, 42-51 are associated with a variety of respiratory disease, generally in children and immunocompromised persons; whereas serotypes 40 and 41 is associated with enteric disease (enteric adenovirus) particularly in children - these enteric adenoviruses will not be detected with the assay described here. Serotypes 12, 18, 31 have a high oncogenic potential and serotypes 4 and 7 are associated with acute respiratory disease (ARD) frequently in military recruits and typically occur in the winter and spring. Smaller outbreaks of serotypes 3, 4, and 7 occur in the summertime and are associated with contaminated swimming pool water.. Adenovirus infections of the eye may lead to pharyngo-conjunctival fever, follicular ...
Human adenoviruses (HAdVs) cause a wide range of diseases worldwide, including respiratory infections. Studies on HAdV molecular epidemiology are limited in Cameroon. The purpose of this study is to document the different types HAdV circulating in Cameroon in children with acute respiratory infections. Nasopharyngeal swabs were collected from 811 children under 15 years from 2011 to 2014. The HAdV detection was assessed by semi-quantitative generic PCR r-gene®. The HAdV-positive samples were typed by amplification and sequencing of partial hexon gene and a real-time PCR. Demographic data were collected and analyzed. The infection and hospitalization risk factors were assessed thought the Chi-square test. A total of 137/220 HAdV-positive samples were amplified successfully. Six species of HAdV (Mastadenovirus A to F) were detected with B (108/220) and C (47/220) being the predominant strains. Hospitalization and age were significantly associated to HAdV-B and HAdV-C respectively. Phylogenetic analysis
Adenovirus type 37 (Ad37) is a leading cause of epidemic keratoconjunctivitis (EKC), a severe and highly contagious ocular disease. Whereas most other adenoviruses infect cells by engaging CD46 or the coxsackie and adenovirus receptor (CAR), Ad37 binds previously unknown sialic acid-containing cell surface molecules. By glycan array screening, we show here that the receptor-recognizing knob domain of the Ad37 fiber protein specifically binds a branched hexasaccharide that is present in the GD1a ganglioside and that features two terminal sialic acids. Soluble GD1a glycan and GD1a-binding antibodies efficiently prevented Ad37 virions from binding and infecting corneal cells. Unexpectedly, the receptor is constituted by one or more glycoproteins containing the GD1a glycan motif rather than the ganglioside itself, as shown by binding, infection and flow cytometry experiments. Molecular modeling, nuclear magnetic resonance and X-ray crystallography reveal that the two terminal sialic acids dock into two of
RNA molecules from nuclear and cytoplasmic polyribosomes of adenovirus-infected HeLa cells were compared by hybridization to analyse the sequence content. Nuclear polyribosomes were released by exposure of intact detergent-washed nuclei to poly(U) and purified. Cytoplasmic polyribosomes were also purified from the same cells. To show that nuclear polyribosomes contain ribosomes linked by mRNA, polyribosomes were labelled with methionine and uridine in the presence of actinomycin D in adenovirus-infected cells. Purified nuclear polyribosomes were treated with EDTA under conditions which dissociate polyribosomes into ribosomes and subunits with a simultaneous release of mRNA, and sedimented. The treatment dissociated these polyribosomes, releasing the mRNA from them. Radiolabelled total RNA from each polyribosome population was fractionated in sucrose gradients into several pools or hybridized to intact adenovirus DNA to select virus-specific RNA. Sucrose-gradient-fractionated pool-3 RNA (about ...
The utility of recombinant adenovirus serotype 5 (rAd5) vector-based vaccines for HIV-1 and other pathogens will likely be limited by the high prevalence of pre-existing Ad5-specific neutralizing Abs (NAbs) in human populations. However, the immunodominant targets of Ad5-specific NAbs in humans remain poorly characterized. In this study, we assess the titers and primary determinants of Ad5-specific NAbs in individuals from both the United States and the developing world. Importantly, median Ad5-specific NAb titers were ,10-fold higher in sub-Saharan Africa compared with the United States. Moreover, hexon-specific NAb titers were 4- to 10-fold higher than fiber-specific NAb titers in these cohorts by virus neutralization assays using capsid chimeric viruses. We next performed adoptive transfer studies in mice to evaluate the functional capacity of hexon- and fiber-specific NAbs to suppress the immunogenicity of a prototype rAd5-Env vaccine. Hexon-specific NAbs were remarkably efficient at ...
The high prevalence of pre-existing anti-Ad5 immunity in human populations may substantially limit the immunogenicity and clinical utility of rAd5 vector-based vaccines for HIV-1 and other pathogens. Our studies demonstrate ,90% Ad5 seroprevalence in sub-Saharan Africa with median NAb titers ,10-fold higher than those found in the United States. These data suggest that rAd5 vectors should be engineered to evade dominant Ad5-specific NAbs before their use as vaccine vectors in the developing world. To determine the principal targets of Ad5-specific NAbs, we exploited the lack of detectable serologic cross-reactivity between Ad5 and Ad35 (8). Virus neutralization studies using capsid chimeric rAd5/rAd35 vectors and serum samples from both humans and mice demonstrated that Ad5-specific NAbs were directed primarily against the Ad5 hexon protein. Fiber-specific NAbs were detected at low frequencies in vitro but were substantially less efficient than hexon-specific NAbs at blunting rAd5 vaccine ...
Last year, Chiu and colleagues also identified another new adenovirus, named simian adenovirus C, which sickened four of nine captive baboons and killed two of them at a primate facility in 1997. Several staff members at the facility also complained of upper respiratory symptoms at the time of the outbreak. Re-examining the samples many years later, Chiu and his colleagues found antibodies targeted to simian adenovirus C in the human samples.. Chiu concluded that staff members had been exposed to the new virus, and that the virus may have jumped from baboon to human, an idea also supported by follow-up experiments in which laboratory strains of simian adenovirus C efficiently infected both human and baboon cells.. Adenoviruses to date have not generally been linked to cross-species infections between monkeys and humans, Chiu said.. In light of these findings, however, he said the normal vigilance in tracking animal viruses that might also infect humans should extend beyond influenza and ...
Seventy-eight cases were identified in patients from four eye care practices, two family practices, and the hospital emergency department. The median patient age was 45 years (range = 9 months-90 years), and 33 (42%) were men. Ocular signs and symptoms included redness (68%), watery discharge (50%), and pain (29%). Severe signs included corneal infiltrates (17%) and pseudomembranes (6%). At least 12 cases (15%) were health care-associated (Figure). One health care-associated case occurred in a health care worker. Seventeen patients whose infections were not health care-associated reported a symptomatic household or community contact. Among 45 conjunctival swabs available for testing, 19 (42%) were positive for HAdV-8. Genome sequences obtained from five HAdV-8 isolates were 100% identical with one another and showed 97.7% (accession number AB861610.1) to 99.9% (accession number KT340070.1) nucleotide sequence similarity to other HAdV-8 genome sequences available in GenBank, the National ...
Mature human adenovirus particles contain four minor capsid proteins, in addition to the three major capsid proteins (penton base, hexon and fiber) and several proteins associated with the genomic core of the virion. Of the minor capsid proteins, VI plays several crucial roles in the infection cycle of the virus, ... read more including hexon nuclear targeting during assembly, activation of the adenovirus proteinase (AVP) during maturation and endosome escape following cell entry. VI is translated as a precursor (pVI) that is cleaved at both N- and C-termini by AVP. Whereas the role of the C-terminal fragment of pVI, pVIc, is well established as an important co-factor of AVP, the role of the N-terminal fragment, pVIn, is currently elusive. In fact, the fate of pVIn following proteolytic cleavage is completely unknown. Here, we use a combination of proteomics-based peptide identification, native mass spectrometry and hydrogen-deuterium exchange mass spectrometry to show that pVIn is associated ...
Subgroup C adenoviruses, including serotypes 2 and 5, from which most therapeutic adenoviruses are derived, rely on CAR as the primary binding site on the host cell. This receptor has been shown to be crucial for sufficient virus uptake (22) . In cancer cells, however, CAR expression is frequently lost, especially in highly malignant cancer cell lines, leading to a significant decrease in adenovirus uptake (5 , 6) . Our own observations are in agreement with these reports: we frequently found reduced CAR expression at the cell surface in high-grade primary liver cancer and metastases of colorectal cancer. 4 This study investigates the molecular mechanisms involved in reducing CAR expression in cancer cells and explores the possibility of pharmacologically manipulating CAR expression levels.. Increasing evidence exists for a potential physiological role of CAR as a cell adhesion molecule. CAR forms homodimers, was found to physically interact with the tight-junction protein ZO-1, and participates ...
Definition of Adenovirus E3 10.4K/14.5kD Protein in the Legal Dictionary - by Free online English dictionary and encyclopedia. What is Adenovirus E3 10.4K/14.5kD Protein? Meaning of Adenovirus E3 10.4K/14.5kD Protein as a legal term. What does Adenovirus E3 10.4K/14.5kD Protein mean in law?
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To delineate the function of adenovirus early region 4 (E4) gene products, we constructed a set of mutant viruses which carry defined lesions within this coding region. Deletion and insertion mutations within six of seven known E4 coding regions had no measurable effect on virus growth in cultured cells. A variant carrying a deletion within the last coding region (encoding a 34,000-molecular-weight polypeptide) was modestly defective, and a mutant lacking the majority of the E4 region was severely defective for growth. The phenotypes of the two defective mutants are similar and complex. Both display perturbations in DNA replication, translation of the E2A mRNA, accumulation of late viral mRNAs, and host cell shutoff. ...
86 PCR positive samples were inoculated onto Hep-2 cell for virus isolation, and 23 HAdV strains were isolated through 7-21 days post-inoculation, with isolation rate of 26.74%, which was close to that of 21.43% in the previous study (Thounaojam et al. 2016). The hexon gene sequence has widely been used for the classification of adenovirus types. In this study, the hexon gene of the 23 HAdV isolates was amplified and sequenced using primer designed by Sarantis et al. (Sarantis et al. 2004) (Forward primer: 5′-CTGATGTACTACAACAGCACTGGCAACATGGG-3′, Reverse primer: 5′-GCGTTGCGGTGGTGGTTAAATGGGTTTACGTTGTCCAT-3′, the amplicon length is 580-605 bp), and the nucleotide sequences were submitted to GenBank (accession numbers MN389388-MN389410). Molecular typing assignments were based on the identity of the closest matching sequences after both BLAST and phylogenetic analysis. Results showed that 9 of the 23 HAdV isolates (8 HAdV-3 and 1 HAdV-7) belonged to species B and 12 of the 23 (5 HAdV-1 and 7 ...
The 293 cell line was derived from primary cultures of human embryonic kidney (HEK) cells with sheared fragments of adenovirus (Ad) 5 DNA (Graham et al. 1977). HEK 293 cells contain the nucleotides 1-4344 of Ad5 which are located within the pregnancy-specific ß-1-glycoprotein 4 (PSG 4) gene. The transforming region of the human adenovirus contains the early region (E1), comprising two transcription units, E1a and E1b, whose products are essential and sufficient for mammalian cell transformation by adenoviruses (Louis et al. 1997). Because 293 cells express E1 gene products they are extensively used for the production of E1-deleted Ad viruses. Adeno-associated viruses (AAVs) belong to the family of Parvoviridae, being one of the smallest single-stranded and non-enveloped DNA viruses. AAVss are replication-deficient and have required co-infection with a helper adeno- or herpes virus for productive infection. The AAV Helper-free system takes advantage of the identification of the specific ...
Purification of recombinant adenovirus type 3 dodecahedric virus-like particles for biomedical applications using short monolithic columns
TY - JOUR. T1 - Biobased monoliths for adenovirus purification. AU - Fernandes, Cláudia S. M.. AU - Gonçalves, Bianca. AU - Sousa, Marcos F. Q.. AU - Martins, Duarte L.. AU - Barroso, Telma. AU - Pina, Ana Sofia. AU - Peixoto, Cristina. AU - Aguiar-Ricardo, Ana. AU - Roque, A. Cecília A.. N1 - info:eu-repo/grantAgreement/FCT/COMPETE/132972/PT# info:eu-repo/grantAgreement/FCT/3599-PPCDT/118317/PT# info:eu-repo/grantAgreement/FCT/3599-PPCDT/135802/PT# Sem PDF conforme despacho.. PY - 2015/4/1. Y1 - 2015/4/1. N2 - Adenoviruses are important platforms for vaccine development and vectors for gene therapy, increasing the demand for high titers of purified viral preparations. Monoliths are macroporous supports regarded as ideal for the purification of macromolecular complexes, including viral particles. Although common monoliths are based on synthetic polymers as methacrylates, we explored the potential of biopolymers processed by clean technologies to produce monoliths for adenovirus purification. ...
Adenoviruses were identified as unique viruses in 1953 by Rowe and coworkers when virus was isolated from human adenoids. Because adenoviruses infect various cells they are considered an excellent vector delivery system. A major obstacle to efficient infection by recombinant adenoviruses is the humo.... Full description. ...
Yakimovich, A; Gumpert, H; Burckhardt, C J; Lutschg, V A; Jurgeit, A; Sbalzarini, I F; Greber, U F (2012). Cell-free transmission of human adenovirus by passive mass transfer in cell culture simulated in a computer model. Journal of Virology, 86(18):10123-10137.. Cardinale, J; Paul, G; Sbalzarini, I F (2012). Discrete region competition for unknown numbers of connected regions. IEEE transactions on image processing : a publication of the IEEE Signal Processing Society, 21(8):3531-3545.. Helmuth, J A; Burckhardt, C J; Greber, U F; Sbalzarini, I F (2009). Shape reconstruction of subcellular structures from live cell fluorescence microscopy images. Journal of Structural Biology, 167(1):1-10.. Helmuth, J A; Burckhardt, C J; Koumoutsakos, P; Greber, U F; Sbalzarini, I F (2007). A novel supervised trajectory segmentation algorithm identifies distinct types of human adenovirus motion in host cells. Journal of Structural Biology, 159(3):347-358.. ...
ViroTag® ADVX (for manual sampling) utilizes a fluorescently-labeled, high-affinity antibody which binds to a unique epitope specifically expressed on adenovirus. The product has been shown to quantify multiple serotypes (2-6) and is considered pan-reactive. With the Virus Counter 3100, use this rapid, no-wash labeling procedure and take adenovirus quantification to new levels of accuracy, speed and simplicity!. Product specifications: The ViroTag ADVX kit (catalog number 92097) contains all reagents and consumables necessary to analyze 200 samples using the Virus Counter 3100 instrument for manual sampling, including:. ...
This 2008 Molecular Cancer Therapeutics paper by Yacoub, A., etc. utilizes the following products and services from Vector Biolabs: Akt1 (dn) Adenovirus, CrmA Adenovirus, Caspase 9 (dn) Adenovirus, human BCL-xL shRNA Adenovirus, Baculoviral IAP repeat-containing 4 Adenovirus, CASP8 and FADD-like apoptosis regulator Adenovirus, MEK1 (dn) Adenovirus.
Adenovirus and pregnancy - What is adenovirus infection? Many forms. This virus can produce several forms of infection. Pharyngo-conjunctival fever (or swimming pool fever) is one of the better known, but also gastroenteritis, upper respiratory tract infections and pneumonia can all occur.
Adenovirus Early Proteins: Proteins encoded by adenoviruses that are synthesized prior to, and in the absence of, viral DNA replication. The proteins are involved in both positive and negative regulation of expression in viral and cellular genes, and also affect the stability of viral mRNA. Some are also involved in oncogenic transformation.
Trentin, J J. and Bryan, E, Immunization of hamsters and histoisogenic mice against trans- plantation of tumors induced by human adenovirus type 12. Abstr. (1964). Subject Strain Bibliography 1964. 1087 ...
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Berget, S.M., Sharp, P.A. (1977) A spliced sequence at the 5′-terminus of adenovirus late mRNA. Brookhaven Symp Biol, 29:332-44.. Berk, A.J., Sharp, P.A. (1977) Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell 12(3): 721-32.. Chow, L.T., Roberts, J.M., Lewis, J.B., Broker, T.R. (1977) A map of cytoplasmic RNA transcripts from lytic adenovirus type 2, determined by electron microscopy of RNA:DNA hybrids. Cell, 11(4): 819-36.. Gibbs, W.W. (2003) The unseen genome: gems among the junk. Scientific American 289(5):26-33.. Hooks, K. B., Delneri, D. & Grifths-Jones, S. (2014) Intron evolution in Saccharomycetaceae. Genome Biol. Evol. 6, 2543-2556.. Kabat, J.L., Barberan-Soler, S., McKenna, P., Clawson, H., Farrer, T., and Zahler, A.M. (2006) Intronic Alternative Splicing Regulators Identified by Comparative Genomics in Nematodes. PLoS Computational Biology 2(7):734-747.. Kiss, T. and Filipowicz, W. (1995) Genes and Development ...
Adenovirus titration kits accurately measure functional titer of your adenovirus preparation using a hexon antibody, or by SYBR Green-based, real-time qPCR in which the virus samples Ct is compared to a standard curve.
Adenovirus titration kits accurately measure functional titer of your adenovirus preparation using a hexon antibody, or by SYBR Green-based, real-time qPCR in which the virus samples Ct is compared to a standard curve.
A HIV-1 DNA prime-recombinant Adenovirus Type 5 (rAd5) increase vaccine failed to guard against HIV-1 acquisition. is certainly towards the gp41 subunit from the envelope (Env) glycoprotein from the pathogen (1). This antibody response derives from polyreactive B cells that cross-react with Env and intestinal microbiota (IM) (2, 3). Nevertheless, it is unidentified if an identical gp41-reactive Ab response would take place in the placing of HIV-1 Env vaccination. A DNA leading, recombinant adenovirus serotype 5 (rAd5) increase vaccine that included HIV and genes, and a trivalent combination of clade A, B and C gp140 genes formulated with both gp120 and gp41 elements was examined in the HIV Vaccine Studies Network (HVTN) [stage Ib (HVTN 082), GW-786034 stage II (HVTN 204), stage IIb (HVTN 505) efficiency trial] and various other clinical studies [stage I/II (RV172), stage I (V001)] (4C7). This vaccine was the GW-786034 initial vaccine formulated with the ectodomain from the Env gp41 component, ...
Cao W, Baniecki ML, McGrath WJ, Bao C, Deming CB, Rade JJ, Lowenstein CJ, Mangel WF. Nitric oxide inhibits the adenovirus proteinase in vitro and viral infectivity in vivo. FASEB J. 2003 Dec; 17(15):2345-6 ...
DNA-protein complex is prepared from the virus (26) and then digested with EcoRI. To increase the probability of obtaining recombinant viruses relative to wt viruses, we added a restriction digest/Klenow fillin step to the preparation of DNA-protein complex prior to transfection (1,4). We also describe the preparation of MAV-1 viral DNA (see Note 6). In the second section we describe the transfection protocol. We have successfully used mouse 3T6 cells to obtain mutants. In some cases we have also used 3T6 cell derivatives, which inducibly express the gene region to be mutated (3,4). 27), but other methods may be used. Any of a variety of methods can be used to obtain the mutation in a plasmid containing the gene of interest, including oligonucleotide-directed mutagenesis, polymerase chain reaction (PCR) mutagenesis, and restriction fragment replacement cloning. In the third section we describe the identification and plaque purification of mutants. We have used preparation of viral DNA directly ...
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A common helper virus in humans is the adenovirus.[citation needed] Dependoparvovirus is not infectious enough to trigger an ... Viral vectors are currently being developed to transport genes into human cells. Since this virus does not stimulate an immune ... they cannot replicate productively in their host cell without the cell being coinfected by a helper virus such as an adenovirus ...
Human herpes virus 8 (HHV8) is associated with four rare lymphoproliferative disorders: 1) a subset of diffuse large B cell ... adenovirus, or toxoplasma. HIV, rubella, and Hepatitis viruses A, B, and C can produce an illness resembling IM. The acute EBV ... The Epstein-Barr virus (also termed human herpesvirus 4) belongs to the Herpes family of Group I double-stranded DNA viruses. ... HRS cells also express the virus's LMP2A gene protein product which mimics the human BCR gene product) in promoting the ...
However, the widespread seroprevalence of neutralizing antibodies to common human adenovirus serotypes limits their use. Simian ... The vector is a chimpanzee adenovirus modified to avoid its replication. Adenoviruses are effective vectors for inducing and ... "A Novel Chimpanzee Adenovirus Vector with Low Human Seroprevalence: Improved Systems for Vector Derivation and Comparative ... orf6 and orf6/7 genes for those from human adenovirus HAdV-C5. It has been demonstrated that the adenoviridae vector ChAdOx1 ...
Pines, Jonathon; Hunter, Tony (1990). "Human cyclin A is adenovirus E1A-associated protein p60 and behaves differently from ... Jonathon Pines publications indexed by Google Scholar Draviam, Viji Mythily (2002). Studies on human B- type cyclins. cam.ac.uk ... Subsequently he cloned and characterised the first human cyclins with Tony Hunter. This was crucial to recognising that cyclins ... and identified the first link between cyclins and oncoproteins by showing that cyclin A bound to adenovirus E1A, thus linking ...
... of subgroup C human adenoviruses, in species with a deficient CAAT sequence. The transcription initiation at mutant MLP species ... "Functional Analysis of the CAAT Box in the Major Late Promoter of the Subgroup C Human Adenoviruses". Journal of Virology. 72 ( ... In another experiment performed with the major late promoter (MLP) of adenoviruses from a variety of host species, it was shown ... The failure to restore the normally functional adenoviruses, exhibited by a CAAT box, is consistent with the idea that the CAAT ...
Infection by human adenovirus accounts for 65% to 90% of cases of viral conjunctivitis. Adenoviruses are the most common cause ... "Molecular epidemiology of circulating human adenovirus types in acute conjunctivitis cases in Chandigarh, North India". Indian ... An adenovirus was first isolated by Rowe et al. in 1953. Two years later, Jawetz et al. published on epidemic ... of Health & Human Services, Centers for Disease Control and Prevention. p. 112. ISBN 978-0990449119. Zentani A, Burslem J ( ...
E19 from some adenoviruses block the movement of MHC I to the proper locations for the endogenous pathway. Nef from some HIV ... U21 from some human herpesvirus 7 binds and targets certain MHC I molecules for lysosomal degradation. ...
Other agents that cause this illness include human metapneumovirus, influenza, parainfluenza, coronavirus, adenovirus, ... Bronchiolitis is usually the result of infection by respiratory syncytial virus (72% of cases) or human rhinovirus (26% of ... This is most commonly caused by respiratory syncytial virus (RSV, also known as human pneumovirus). ...
"Serotype chimeric oncolytic adenovirus coding for GM-CSF for treatment of sarcoma in rodents and humans". International Journal ... Yang, J.-L.; Hannan, M.T.; Russell, P.J.; Crowe, P.J. (2006). "Expression of HER1/EGFR protein in human soft tissue sarcomas". ... It is modified to selectively replicate in p16/Rb-defective cells, which include most human cancer cells. In addition, CGTG-102 ... While the CGTG-102 oncolytic adenovirus has shown efficacy as a single agent against several soft tissue sarcomas, it would ...
Viral causes include human respiratory syncytial virus (RSV), human metapneumovirus, adenovirus, human parainfluenza viruses, ... The most common viral causes are influenza, parainfluenza, human respiratory syncytial virus, human metapneumovirus and ... adenovirus. Less common viruses which may cause serious illness include chickenpox, SARS, avian flu and hantavirus. Typically, ...
Mobility and survival of Salmonella Typhimurium and human adenovirus from spiked sewage sludge applied to soil columns (2010). ...
"Study of Coxsackie B viruses interactions with Coxsackie Adenovirus receptor and Decay-Accelerating Factor using Human CaCo-2 ... Riabi, 2014) When VP1 binds to the Coxsackie-Adenovirus receptor (CAR), which can be found on heart muscle cells as well as ... Dorner, A. A. (2005). "Coxsackievirus-adenovirus receptor (CAR) is essential for early embryonic cardiac development". Journal ... but to date there is no stringent evidence to support this hypothesis in humans. A 2004 systematic review analyzing a possible ...
"Accurate identification of neutralizing antibodies to adenovirus Ad36, -a putative contributor of obesity in humans". Journal ... Accurate identification of neutralizing antibodies to adenovirus Ad36, -a putative contributor of obesity in humans. J Diabetes ... Specificity and sensitivity of commercially available assays for glucagon and oxyntomodulin measurement in humans. Eur J ... "Specificity and sensitivity of commercially available assays for glucagon and oxyntomodulin measurement in humans". European ...
Viruses often mimic human SLiMs to hijack and disrupt a host's cellular machinery, thereby adding functionality to their ... the Adenovirus protein E1A. Pathogenic bacteria also mimic host motifs (as well as having their own motifs), however, not to ... Usher's Syndrome is the most frequent cause of hereditary deaf-blindness in humans and can be caused by mutations in either PDZ ... Kadaveru K, Vyas J, Schiller MR (May 2008). "Viral infection and human disease--insights from minimotifs". Frontiers in ...
Transformation and oncogenicity by human adenoviruses. He then moved to the United States and was a post doc under Robert ...
In addition, DPPC has been shown to be related to infection of polarized cells by a specific kind of human adenovirus (HAdV-C2 ... However, adsorption is not optimal at human body temperature for DPPC alone, because at 37 °C it is found in a gel phase. The ... This phospholipid is found in a solid/gel phase at 37 °C (at the effective temperature of the human body). Its melting point is ... It also plays an important role in the study of liposomes and human bilayers. Lung surfactant (LS) is a surface-active material ...
"ICTV Taxonomy history: Human coxsackievirus A4". International Committee on Taxonomy of Viruses (ICTV). Retrieved 6 February ... is mediated by Coxsackievirus and adenovirus receptor. Coxsackieviruses are divided into group A and group B viruses based on ... "ICTV Taxonomy history: Human coxsackievirus A6". International Committee on Taxonomy of Viruses (ICTV). Retrieved 6 February ... Cavatak, a wild-type Coxsackievirus A21, is being used in human clinical trials as an oncolytic virus. Pedro-Pons, Agustín ( ...
1-81, doi:10.1002/14356007.a13_089, ISBN 978-3527306732 "Human adenovirus type 26 uses sialic acid-bearing glycans as a primary ... In humans the brain has the highest sialic acid content, where these acids play an important role in neural transmission and ... It has been demonstrated that the human brain has more sialic acid than the brains of other mammals (2 - 4 times more). Neural ... However human milk varies in sialic acid content, depending upon genetic inheritance, lactation, etc. Investigations are ...
Human astroviruses are part of the Mammastrovirus genus and contains 8 serotypes. The human astrovirus capsid spikes have a ... Bennett S, Gunson RN (April 2017). "The development of a multiplex real-time RT-PCR for the detection of adenovirus, astrovirus ... Furthermore, some human, duck, chicken and turkey astroviruses are phylogenetically related and share genetic features. Human ... The human astrovirus genome mutation rate has been estimated to be 3.7×10−3 nucleotide substitutions per site per year with the ...
In 2009 an outbreak of a monkey-killing cold virus identified as an adenovirus infected both monkeys and humans, with the ... was the site of this outbreak in what is being considered the first known case of an adenovirus jumping from monkeys to humans ... dedicated to improving human and animal health, and located on the University of California, Davis, campus. The CNPRC is part ... "Cross-Species Transmission of a Novel Adenovirus Associated with a Fulminant Pneumonia Outbreak in a New World Monkey Colony". ...
... which is a polysaccharide surface antigen on red cells in most humans. As a weak, biphasic antibody, it absorbs to the P ... and adenovirus. Non-viral agents include Mycoplasma pneumoniae and Haemophilus influenzae. Chronic relapsing PCH is classically ...
Carrigan, Phd (1997). "Adenovirus Infections in Immunocompromised Patients". The American Journal of Medicine. 102 (3): 71-74. ... U.S. Department of Health & Human Services. 65 (2): 1-44. doi:10.15585/mmwr.rr6502a1. PMID 27172113. Ryan, Edward; Durand, ... Meningoencephalitis is a rare, late-stage manifestation of tick-borne ricksettial diseases, such as RMSF and Human ... 2008). "Acute meningoencephalitis due to human immunodeficiency virus type 1 infection in 13 patients: clinical description and ...
... adenoviruses, canine MeSH B04.280.030.500.350 - adenoviruses, human MeSH B04.280.030.500.675 - adenoviruses, porcine MeSH ... adenoviruses, canine MeSH B04.909.204.097.500.350 - adenoviruses, human MeSH B04.909.204.097.500.675 - adenoviruses, porcine ... human MeSH B04.820.565.284.182 - enterovirus b, human MeSH B04.820.565.284.182.225 - echovirus 6, human MeSH B04.820.565.284. ... human MeSH B04.909.777.618.284.182 - enterovirus b, human MeSH B04.909.777.618.284.182.225 - echovirus 6, human MeSH B04.909. ...
Human influenza virus had first been isolated just a few years earlier. Later, Hirst also studied other vertebrate RNA viruses ... adenoviruses, polyomaviruses and arboviruses, and is still widely used in influenza surveillance and vaccine testing. Hannah ... In 1940, at the Rockefeller Foundation, he started to work on influenza viruses - enveloped RNA viruses infecting humans, birds ... Hirst GK, Rickard ER, Whitman L, Horsfall FL, Jr (1942). "Antibody response of human beings following vaccination with ...
... human immunodeficiency virus and DNA tumor viruses (adenoviruses, SV-40). The drive towards practical applications of the ... to excise the DNA of the human immunodeficiency virus from the genome of individual cells to remove it. This demonstration is ... human T-cell leukemia virus-1, murine leukemia virus), coronaviruses, ...
A fat virus is the popular name for the notion that some forms of obesity in humans and animals have a viral source. Causes of ... obesity Health implications of Firmicutes Obesogen Systemic inflammation and obesity Adenovirus serotype 36 DiBaise JK, Zhang H ... Gut flora has been shown to differ between lean and obese humans. There is an indication that gut flora in obese and lean ... Humans are unable to digest complex polysaccharides and rely on gut microbiota to ferment these polysaccharides into short ...
If safety issues can be resolved, gene therapy may provide an alternative human treatment for MPS disorders in the future. ... In animal models, delivery of the iduronidase gene has been accomplished with retrovirus, adenovirus, adeno-associated virus, ...
Cell-Based Therapeutics: Contributed extensively on genetic modification of human islets for improved islet transplantation. ... Construction of Plasmid and Adenovirus-based Gene and shRNA Expression Systems. These systems are being tested in various ...
In addition, a human diet is not ideal for a dog: the concept of a "balanced" diet for a facultative carnivore like a dog is ... using canine adenovirus type 2 to avoid reaction). The decision on whether to vaccinate against other diseases, including ... Just as in humans, a dog's diet must consist of the appropriate mix of nutrients, carbohydrates, and proteins to give them the ... While not all human delicacies are acutely toxic to dogs (see above), many have the same chronically unfortunate results as ...
August 2009). "Inactivation of animal and human prions by hydrogen peroxide gas plasma sterilization". Infection Control and ... "Molecular indications of protein damage in adenoviruses after UV disinfection". Applied and Environmental Microbiology. 77 (3 ... Department of Health and Human Services. 2004. Cite journal requires ,journal= (help) Onyango LA, Dunstan RH, Roberts TK (May ... as the properties that make chemicals effective sterilants usually make them harmful to humans. The procedure for removing ...
This review attempts to address the importance of developing pharmaceuticals for adenovirus and also review recent development ... in drug discovery for adenovirus, including newer strategies such as microRNA approaches. Different drug screening strategies ... many stages in the development of a new drug for viral infection and such processes are even further complicated for adenovirus ... Adenovirus Biology and Structure. An adenovirus is a non-enveloped, dsDNA virus. There are at least 51 human Adenovirus ...
DNA from Human adenovirus 4 strain RI-67 [ATCC ® VR-1572™] Application: It is suitable for use in polymerase chain reaction ( ... DNA from Human adenovirus 4 strain RI-67 [ATCC® VR-1572™] (ATCC® VR-1572D™) Organism: Human adenovirus 4 / Focus: Infectious ... DNA from Human adenovirus 4 strain RI-67 [ATCC® VR-1572™] ATCC® VR-1572D™ frozen 100 µL per vial ... infected with Human adenovirus 4 (HAdV-4) strain RI-67, (ATCC® VR-1572™). It is supplied at 100 µL per vial, shipped frozen. It ...
DNA from Human adenovirus 12 strain Huie [ATCC ® VR-863™] Application: It is suitable for use in polymerase chain reaction (PCR ... DNA from Human adenovirus 12 strain Huie [ATCC® VR-863™] (ATCC® VR-863D™) Organism: Human adenovirus 12 / Focus: Infectious ... infected with Human adenovirus 12 (HAdV-12) strain Huie (ATCC® VR-863™). It is supplied at 100 µL per vial, shipped frozen. It ...
Drug: Recombinant Human Adenovirus Type 5 Injection After identifying the target artery of HCC, Recombinant Human Adenovirus ... Recombinant Human Adenovirus Type 5, an E1B gene deleted adenovirus, is known to have a significant antitumor activity. In ... Experimental: TACE Plus Adenovirus After identifying the target artery of HCC, Recombinant Human Adenovirus Type 5 Injection( ... TACE Plus Recombinant Human Adenovirus for Hepatocellular Carcinoma. Official Title ICMJE Phase Ⅲ Trial of Transcatheter ...
Human adenovirus 12. Other names i. ›Adenovirus Ad12. ›Adenovirus type 12. ›Human adenovirus type 12. ›Mastadenovirus 12. › ... Taxonomy - Human adenovirus A serotype 12 (HAdV-12) (Human adenovirus 12) Basket 0 ...
Immunological Relationships Between Hexons of Certain Human Adenoviruses Message Subject (Your Name) has forwarded a page to ... Immunological Relationships Between Hexons of Certain Human Adenoviruses. Erling Norrby, Göran Wadell ... were absorbed with purified hexons of various serotypes representing the different subgroups of human adenoviruses. Group, ... This question is for testing whether or not you are a human visitor and to prevent automated spam submissions. ...
2. Human adenovirus infection counteracts the anti-viral activity of the cellular MKRN1 E3 ubiquitin ligase. Open this ... Human adenovirus (HAdV) is a common pathogen causing a broad spectrum of diseases. HAdV encodes the pVII protein, which is ... 4. RNA triplex formation in human adenovirus type 4 VA RNAI and its implication on virus growth. Open this publication in new ... Open this publication in new window or tab ,,Complementation of the human adenovirus type 5 VA RNAI defect by the Vaccinia ...
Crystallographic structure at 1.6-A resolution of the human adenovirus proteinase in a covalent complex with its 11-amino-acid ... CRYSTAL STRUCTURE OF HUMAN ADENOVIRUS 2 PROTEINASE WITH ITS 11 AMINO ACID COFACTOR AT 1.6 ANGSTROM RESOLUTION. ...
... we tracked vectors derived from the human adenovirus type 5 at whole body, tissue and cellular scales throughout the digestive ... we tracked vectors derived from the human adenovirus type 5 at whole body, tissue and cellular scales throughout the digestive ... Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J. Gen. Virol. 36, 59-74. doi: 10.1099/ ... Among the non-replicative viral vectors that have been explored as vaccine carriers, those derived from human adenovirus type 5 ...
1.Production of transgenic mice carrying human renin promoter/E1A and E1B genes of human adenovirus type 12 / p12 (0020.jp2) ... Molecular mechanism of tumorigenesis in transgenic mice carrying E1A and E1B genes of human adenovirus * * 杉山, 文博 スギヤマ, フミヒロ ... Neuroectodermal Tumors Expressing c-,L-,and N-myc in Transgenic Mice That Carry the E1A/E1B Gene of Human Adenovirus Type 12 ... 2.Carcinoid tumor in the transgenic mice carrying E1A and E1B genes of human adenovirus type 12 / p12 (0020.jp2) ...
... although apparent recombinant strains of other viruses from species Human adenovirus D (HAdV-D) have been described. The ... Bootscanning analysis of the complete genomic sequence of this novel adenovirus, which we have re-named HAdV-D53, indicated at ... Bioinformatic and phylogenetic analyses of this genome demonstrate that this adenovirus is a recombinant of HAdV-D8 (including ... and biological descriptions of the molecular evolution events engendering an emerging pathogenic adenovirus. ...
... structure of the complex between the trimeric human adenovirus B serotype 3 fibre knob and human desmoglein 2 fragments ... structure of the complex between the trimeric human adenovirus B serotype 3 fibre knob and human desmoglein 2 fragments ... hus Pelka Human adenovirus infection is driven by Early region 1A (E1A) proteins, which are the first proteins expressed ... Intermediate-resolution crystal structure of the human adenovirus B serotype 3 fibre knob in complex with the EC2-EC3 fragment ...
National Adenovirus Type Reporting System identifies circulation patterns for human adenovirus. ... National Adenovirus Type Reporting System identifies circulation patterns for human adenovirus. ... Distribution of human adenovirus species (HAdVs) and types, by year of specimen collection* - National Adenovirus Type ... The figure above is a bar chart showing the distribution of human adenovirus species and types by the National Adenovirus Type ...
Enhanced expression of p53 in human cells infected with mutant adenoviruses.. Grand RJ1, Grant ML, Gallimore PH. ... The expression of p53 in human cells infected with wild-type (wt) and mutant adenoviruses has been examined. With wt Ad5 and ... the concentration of p53 increased markedly to levels comparable to those seen in adenovirus transformed cells. This increase ...
... Scientific Reports 3:1812. Abstract. The recent emergence of highly virulent human ... suggesting foci of DNA instability lead to formulaic patterns of homologous recombination and confer agility to adenovirus ... adenoviruses (HAdVs) with new tissue tropisms underscores the need to determine their ontogeny. Here we report complete high ...
Computational Analysis Identifies Human Adenovirus Type 55 as a Re-Emergent Acute Respiratory Disease Pathogen Michael P. Walsh ... Quantitative Detection and Rapid Identification of Human Adenoviruses Rika Miura-Ochiai, Yasushi Shimada, Tsunetada Konno, ... Pseudo-Outbreak of Adenovirus Infection in a Neonatal Intensive Care Unit Due to a False-Positive Antigen Detection Test Howard ... Genomic Analyses of Recombinant Adenovirus Type 11a in China Zhaohui Yang, Zhen Zhu, Liuying Tang, Li Wang, Xiaojuan Tan, ...
Rapid and quantitative detection of human adenovirus DNA by real-time PCR.. Heim A1, Ebnet C, Harste G, Pring-Akerblom P. ... Rapid diagnosis of human adenovirus (HAdV) infections was achieved by PCR in the recent years. However, conventional PCR has ... Adenovirus viremia was detected by TaqMan PCR in 4 of 27 (14.8%) paediatric and 8 of 93 (8.6%) adult stem cell transplant ... In conclusion, real-time PCR is a sensitive and quantitative procedure for the detection of adenovirus infections. ...
There are no specific protocols for Recombinant Human Coxsackie Adenovirus Receptor protein (ab114429). Please download our ...
adenovirus;. CVB,. group B coxsackieviruses;. CMV,. cytomegalovirus;. HCAR,. human CVB and Ad2 and 5 receptor;. MCAR,. mouse ... However, adenovirus (Ad) serotypes 2 (Ad2) and 5 (Ad5) and the parental group B coxsackieviruses (CVB) are human pathogens that ... HCAR and MCAR: The human and mouse cellular receptors for subgroup C adenoviruses and group B coxsackieviruses. Richard P. ... HCAR and MCAR: The human and mouse cellular receptors for subgroup C adenoviruses and group B coxsackieviruses ...
Drug: Recombinant Human Adenovirus Type 5 Injection After identifying the target artery of HCC, Recombinant Human Adenovirus ... Recombinant Human Adenovirus Type 5, an E1B gene deleted adenovirus, is known to have a significant antitumor activity. In ... Experimental: TACE Plus Adenovirus After identifying the target artery of HCC, Recombinant Human Adenovirus Type 5 Injection( ... TACE Plus Recombinant Human Adenovirus for Hepatocellular Carcinoma. The safety and scientific validity of this study is the ...
Upon transfection of salivary glands, adenovirus encoding human aquaporin-1 (AdhAQP1) directs human aquaporin-1 (hAQP1) ... recombinant adenovirus encoding human aquaporin-1 with potential membrane water channel activity. ... adenovirus encoding human aquaporin-1 A replication-deficient, recombinant adenovirus encoding human aquaporin-1 with potential ... Upon transfection of salivary glands, adenovirus encoding human aquaporin-1 (AdhAQP1) directs human aquaporin-1 (hAQP1) ...
... R. Moreno,1 L. A. ... "Human mesenchymal stem cells lack tumor tropism but enhance the antitumor activity of oncolytic adenoviruses in orthotopic lung ... A. Rosewell Shaw and M. Suzuki, "Recent advances in oncolytic adenovirus therapies for cancer," Current Opinion in Virology, ... R. W. Chan, K. E. Schwab, and C. E. Gargett, "Clonogenicity of human endometrial epithelial and stromal cells," Biology of ...
... a more virulent strain of human adenovirus arose from recombination with other distinct strains of milder human adenoviruses. ... Adenoviruses commonly infect humans, causing colds, flu-like symptoms and sometimes even death, but now UC San Francisco ... "Adenoviruses to date have not generally been linked to cross-species infections between monkeys and humans," Chiu said. ... Last year, Chiu and colleagues also identified another new adenovirus, named simian adenovirus C, which sickened four of nine ...
Presented are ways to address the problem of replication competent adenovirus in adenoviral production for use with, for ... 0030] These cells cannot be used directly for the production of recombinant human adenovirus, as human adenovirus cannot ... The newly generated human adenovirus packaging cell lines or cell lines derived from species permissive for human adenovirus ( ... Source of adenovirus sequences. [0117] Adenovirus sequences are derived either from pAd5.SalB containing nt. 80-9460 of human ...
New human adenovirus isolated from a renal transplant recipient: description and characterization of candiate adenovirus type ... Latent Species C Adenoviruses in Human Tonsil Tissues. C. T. Garnett, G. Talekar, J. A. Mahr, W. Huang, Y. Zhang, D. A. ... Latent Species C Adenoviruses in Human Tonsil Tissues. C. T. Garnett, G. Talekar, J. A. Mahr, W. Huang, Y. Zhang, D. A. ... Latent Species C Adenoviruses in Human Tonsil Tissues. C. T. Garnett, G. Talekar, J. A. Mahr, W. Huang, Y. Zhang, D. A. ...
... transformed with adenovirus 5 DNA ) from GeneTex,Human 293 cells were cultured in Minimum Essential Medium (Eagle) with 2 mM L- ... A - 549 cell line slides ( human: lung; carcinoma ) from GeneTex. 11. Raji cell line slides ( human: B lymphocyte; Burkitts ... 293 cell line slides ( human: kidney; transformed with adenovirus 5 DNA ) from GeneTex. ... Human 293 cells were cultured in Minimum Essential Medium (Eagle) with 2 mM L-glutamine and harvested at the log phase of ...
Human adenovirus B serotype 3 (HAdV-3) (Human adenovirus 3). ,p>This subsection of the ,a href="http://www.uniprot.org/help/ ... sp,P04501,SPIKE_ADE03 Fiber protein OS=Human adenovirus B serotype 3 OX=45659 GN=L5 PE=1 SV=1 ... IPR008982 Adenovirus_pIV-like_att. IPR009013 Attachment_protein_shaft_sf. Pfami. View protein in Pfam. PF00541 Adeno_knob, 1 ... Viruses › dsDNA viruses, no RNA stage › AdenoviridaeMastadenovirusHuman mastadenovirus B. ,p>This subsection of the ,a ...
These diseases associated with adenovirus infection affect adults and are usually more severe in infant ... Adenoviruses are known to cause several human diseases including acute febrile respiratory syndromes, epidemic conjunctivitis ... Forty-seven human adenoviruses serotypes have so far been identified adenovirus. The diversity of these viruses has delayed ... kit designed for diagnosis of human enteric adenoviruses in stool samples.. RESULTS: The highest prevalence of adenoviruses, ...
... but now UC San Francisco researchers have discovered that a new species of adenovirus can spread from primate to primate, and ... Adenoviruses commonly infect humans, causing colds, flu-like symptoms and sometimes even death, ... Adenoviruses may pose risk for monkey-to-human leap. July 25, 2013, University of California, San Francisco Adenoviruses ... While human adenoviruses typically cause mild infections, recent reports have described newly characterized adenoviruses that ...
... and a replication-deficient adenovirus containing the genomic sequence of human CYP21 (hAdCYP21). Intra-adrenal injection of ... Adenovirus mediated expression of therapeutic plasma levels of human factor IX in mice Nat Genet 1993 5: 397-402 ... Tajima, T., Okada, T., Ma, X. et al. Restoration of adrenal steroidogenesis by adenovirus-mediated transfer of human ... Restoration of adrenal steroidogenesis by adenovirus-mediated transfer of human cytochromeP450 21-hydroxylase into the adrenal ...
EKC is a severe form of acute conjunctivitis caused by human adenoviruses (HAdVs). Clinical illness typically lasts 1 to 3 ... Notes from the Field: Epidemic Keratoconjunctivitis Outbreak Associated with Human Adenovirus Type 8 - U.S. Virgin Islands, ... Notes from the Field: Epidemic Keratoconjunctivitis Outbreak Associated with Human Adenovirus Type 8 - U.S. Virgin Islands, ... or laboratory confirmation of human adenovirus type 8 from a specimen collected by conjunctival swab during June 1-November 29 ...
sewage PCR adenoviruses fecal contamination adenovirus JC polyomaviruses water contamination HEV JCV hepatitis E shellfish More ... Effect of temperature and sunlight on the stability of human adenoviruses and MS2 as fecal contaminants on fresh produce ... Effect of temperature and sunlight on the stability of human adenoviruses and MS2 as fecal contaminants on fresh produce ...
Human adenoviruses (Ads) are responsible for a substantial disease burden. Type-specific identification of Ads can help guide ... Gruber, WC, Russell, DJ, Tibbetts, C 1993Fiber gene and genomic origin of human adenovirus type 4Virology196603611PubMed ... Shimada, Y, Ariga, T, Yoshitsugu, T, Aoki, K, Ohno, S, Ishiko, H 2004Molecular diagnosis of human adenoviruses D and E by a ... Pring-Åkerblom, P, Adrian, T, Kostler, T 1997PCR-based detection and typing of human adenoviruses in clinical samplesRes Virol ...
However, little information is available about the gene transfer efficiency in human malignant glioma in vivo. We compared the ... and adenovirus-mediated gene therapy have been suggested as a novel approach to the treatment of malignant brain tumors. ... Beta-galactosidase Gene Transfer to Human Malignant Glioma in Vivo Using Replication-Deficient Retroviruses and Adenoviruses ... We compared the feasibility and safety of retrovirus- and adenovirus-mediated beta-galactosidase gene transfer in human ...
  • To define the bottlenecks that restrict antigen expression after oral administration of viral-vectored vaccines, we tracked vectors derived from the human adenovirus type 5 at whole body, tissue, and cellular scales throughout the digestive tract in a murine model of oral delivery. (frontiersin.org)
  • Of interest is the role of adenoviruses as vectors in vaccination and in gene therapy. (myhospitall.org)
  • The complete genome sequence of human adenovirus shows that it contains large amount of proteins with unknown cellular or biochemical function, known as hypothetical proteins. (biomedcentral.com)
  • We found that these proteins may act as DNA terminal protein, DNA polymerase, DNA binding protein, adenovirus E3 region protein CR1 and adenoviral protein L1. (biomedcentral.com)
  • Human adenovirus early region 4 open reading frame 1 genes encode growth-transforming proteins that may be distantly related to dUTP pyrophosphatase enzymes. (acris-antibodies.com)
  • The 12S E1A protein of human Adenovirus 5 associates with pRb-family proteins via residues in its Conserved Regions (CR) 1 and 2, in particular through the motif LXCXE in CR2. (elsevier.com)
  • Normal human cell proteins that interact with the adenovirus type 5 E1B 55kDa protein. (princeton.edu)
  • Thereafter, adenovirus produces potent E1A proteins that immortalize primary rodent cells by altering cellular transcription, ultimately leading to deregulation of apoptosis and malignant transformation. (myhospitall.org)
  • Antisera against hexons of serotypes 2, 4, 5, and 6 (subgroup III), and 15 (subgroup II) were absorbed with purified hexons of various serotypes representing the different subgroups of human adenoviruses. (asm.org)
  • Recognizes the hexon group antigen present in most adenovirus serotypes. (novusbio.com)
  • NB100-65051 has known reactivity with at least 17 serotypes of Adenovirus including types 40 & 41. (novusbio.com)
  • The many different serotypes of human adenoviruses (Ad) are divided into six subgroups, of which all Ad subgroup A and B and two subgroup D Ads can elicit tumors in infected rodents. (acris-antibodies.com)
  • in humans, more than 50 distinct adenoviral serotypes have been found to cause a wide range of illnesses , from mild respiratory infections in young children (known as the common cold ) to life-threatening multi-organ disease in people with a weakened immune system . (wikipedia.org)
  • CD46 for the group B human adenovirus serotypes and the coxsackievirus adenovirus receptor (CAR) for all other serotypes. (wikipedia.org)
  • Possessing 52 serotypes, adenovirus is recognized as the etiologic agent of various diverse syndromes. (myhospitall.org)
  • Adenovirus can cause various diseases like respiratory tract infection, lung infections, common-cold, conjunctivitis etc. (biologyreader.com)
  • The life cycle of adenovirus is a very complex process which first infects the cell, replicates and multiply within the host cell and finally release to produce various types of infections. (biologyreader.com)
  • The infections of adenovirus are most likely to occur in the late winter, spring and early summer months. (biologyreader.com)
  • Adenovirus can define as the virus which belongs to the group-1 , ds-DNA human virus, which can usually cause acute respiratory infections and occasionally cause conjunctivitis, cystitis and gastroenteritis. (biologyreader.com)
  • It is the mammalian adenovirus, i.e. cause infections in mammals. (biologyreader.com)
  • Adenovirus has been associated with both sporadic and epidemic disease and, with regard to infections among military recruits, who were routinely immunized against types 4 and 7 from 1971 until the cessation of vaccine production in 1996. (myhospitall.org)
  • The quest for an efficacious HIV vaccine has resulted in several clinical trial failures, including the Step Trial, which used a replication-incompetent adenovirus (AdV) vector called human adenovirus type 5 (HAdV-5). (upenn.edu)
  • Adenovirus is often cultured from the pharynx and stool of asymptomatic children, and most adults have measurable titers of anti-adenovirus antibodies, implying prior infection. (myhospitall.org)
  • There are currently no images for Adenovirus Hexon Antibody (NB100-65051G). (novusbio.com)
  • Be the first to review our Adenovirus Hexon Antibody (3G0) [DyLight 488] and receive a gift card or discount. (novusbio.com)
  • Please refer to Custom Recombinant Adenovirus Service for additional details on available virus titer upgrades and pricing. (abmgood.com)
  • Viral Moulds and Cement: How Interactions among Human Adenovirus Hexons and Their Protein IX Cement May Buttress Human Adenovirus Particles. (princeton.edu)
  • Adenovirus is relatively stable virus particles which can survive at a temperature of 37degrees Celsius for about a week. (biologyreader.com)
  • The genome of adenovirus also encodes tripartite RNA leader or TPL sequences. (biologyreader.com)
  • The genome of adenovirus is well known and can be modified with relative ease to induce lysis or cytotoxicity of a specified cell line without affecting others. (myhospitall.org)
  • Upon infection with adenovirus, one of three different interactions with the cells may occur. (myhospitall.org)
  • [ 25 ] In one study involving children who underwent hematopoietic stem cell transplantation, all patients who died of adenoviral infection lacked specific T cells against adenovirus. (medscape.com)
  • This adenovirus is part of abm's Adenoviral Expression System and can be used directly to transiently over-express your gene of interest in a wide range of host cells. (abmgood.com)
  • The purpose of this study is to determine if TACE plus Recombinant Human Adenovirus Type 5 Injection will improve outcome in patients with advanced hepatocellular carcinoma (HCC) not amenable to surgery or local ablative therapy. (clinicaltrials.gov)
  • Recombinant Human Adenovirus Type 5, an E1B gene deleted adenovirus, is known to have a significant antitumor activity. (clinicaltrials.gov)
  • In addition, local injection of recombinant human adenovirus type 5 can enhance the effect of antitumor therapies (chemotherapy and radiotherapy). (clinicaltrials.gov)
  • The hypothesis is that patients with unresectable HCC may benefit from recombinant human adenovirus type 5 in combination with TACE. (clinicaltrials.gov)
  • Human adenovirus type 9-induced rat mammary tumors. (acris-antibodies.com)
  • Adenovirus type 9 E4 open reading frame 1 encodes a transforming protein required for the production of mammary tumors in rats. (acris-antibodies.com)
  • The p53 protein does not facilitate adenovirus type 5 replication in normal human cells. (princeton.edu)
  • Adenovirus is a type of DNA virus, which are medium-sized, non-enveloped and double-stranded. (biologyreader.com)
  • This adenovirus can be used to amplify more adenovirus by transducing HEK293 cells. (abmgood.com)
  • Adenoviruses possess a linear dsDNA genome and are able to replicate in the nucleus of vertebrate cells using the host's replication machinery. (wikipedia.org)
  • It can attack rapidly to the human embryonic kidney, HeLa/HEp-2 cells. (biologyreader.com)
  • The first is lytic infection, which occurs when an adenovirus enters human epithelial cells and continues through an entire replication cycle, which results in cytolysis, cytokine production, and induction of host inflammatory response. (myhospitall.org)
  • Human adenoviruses are small double stranded DNA viruses that provoke vast array of human diseases. (biomedcentral.com)
  • Adenoviruses (members of the family Adenoviridae ) are medium-sized (90-100 nm ), nonenveloped (without an outer lipid bilayer) viruses with an icosahedral nucleocapsid containing a double stranded DNA genome. (wikipedia.org)
  • Adenoviruses represent the largest known nonenveloped viruses. (wikipedia.org)
  • Mechanics of Viral Chromatin Reveals the Pressurization of Human Adenovirus. (princeton.edu)
  • After a 12-year hiatus, the FDA approved a live oral vaccine against adenovirus types 4 and 7 in 2011. (medscape.com)
  • A live oral vaccine against adenovirus types 4 and 7 was approved for use in this population by the US Food and Drug Administration (FDA) in 2011, and subsequent incidence of acute respiratory disease declined. (myhospitall.org)
  • Whereas this observation strictly concerns parenteral delivery routes, orally-delivered vaccines are also sought, owing to their ease of delivery in humans and other species and their potential to elicit mucosal immunity. (frontiersin.org)
  • Based on the Ad5 genome, we have generated three types of telomerase-specific replication-competent oncolytic adenoviruses: OBP-301 (Telomelysin), green fluorescent protein (GFP)-expressing OBP-401 (TelomeScan), and tumor suppressor p53-armed OBP-702. (medworm.com)
  • Adenovirus is isolated most commonly in infants and children. (myhospitall.org)
  • The cryo-electron microscopy (cryo-EM) structure of the complex between the trimeric human adenovirus B serotype 3 fibre knob and human desmoglein 2 fragments containing cadherin domains EC2 and EC3 has been published, showing 3:1 and 3:2 complexes. (medworm.com)
  • Adenovirus is ubiquitous, which can spread worldwide. (biologyreader.com)
  • An extremely hardy virus, adenovirus is ubiquitous in human and animal populations, survives long periods outside a host, and is endemic throughout the year. (myhospitall.org)