Adenovirus Early Proteins: Proteins encoded by adenoviruses that are synthesized prior to, and in the absence of, viral DNA replication. The proteins are involved in both positive and negative regulation of expression in viral and cellular genes, and also affect the stability of viral mRNA. Some are also involved in oncogenic transformation.Adenoviruses, Human: Species of the genus MASTADENOVIRUS, causing a wide range of diseases in humans. Infections are mostly asymptomatic, but can be associated with diseases of the respiratory, ocular, and gastrointestinal systems. Serotypes (named with Arabic numbers) have been grouped into species designated Human adenovirus A-F.DNA Replication: The process by which a DNA molecule is duplicated.DNA, Viral: Deoxyribonucleic acid that makes up the genetic material of viruses.Adenoviridae: A family of non-enveloped viruses infecting mammals (MASTADENOVIRUS) and birds (AVIADENOVIRUS) or both (ATADENOVIRUS). Infections may be asymptomatic or result in a variety of diseases.Adenovirus Infections, Human: Respiratory and conjunctival infections caused by 33 identified serotypes of human adenoviruses.Adenovirus E1A Proteins: Proteins transcribed from the E1A genome region of ADENOVIRUSES which are involved in positive regulation of transcription of the early genes of host infection.Viral Proteins: Proteins found in any species of virus.Adenoviridae Infections: Virus diseases caused by the ADENOVIRIDAE.Adenovirus E1B Proteins: Proteins transcribed from the E1B region of ADENOVIRUSES which are involved in regulation of the levels of early and late viral gene expression.Carcinoma, Non-Small-Cell Lung: A heterogeneous aggregate of at least three distinct histological types of lung cancer, including SQUAMOUS CELL CARCINOMA; ADENOCARCINOMA; and LARGE CELL CARCINOMA. They are dealt with collectively because of their shared treatment strategy.Lung Neoplasms: Tumors or cancer of the LUNG.Radiosurgery: A radiological stereotactic technique developed for cutting or destroying tissue by high doses of radiation in place of surgical incisions. It was originally developed for neurosurgery on structures in the brain and its use gradually spread to radiation surgery on extracranial structures as well. The usual rigid needles or probes of stereotactic surgery are replaced with beams of ionizing radiation directed toward a target so as to achieve local tissue destruction.Neoplasm Staging: Methods which attempt to express in replicable terms the extent of the neoplasm in the patient.Prognosis: A prediction of the probable outcome of a disease based on a individual's condition and the usual course of the disease as seen in similar situations.DNA, Recombinant: Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected.Carcinoma, Small Cell: An anaplastic, highly malignant, and usually bronchogenic carcinoma composed of small ovoid cells with scanty neoplasm. It is characterized by a dominant, deeply basophilic nucleus, and absent or indistinct nucleoli. (From Stedman, 25th ed; Holland et al., Cancer Medicine, 3d ed, p1286-7)Antineoplastic Agents: Substances that inhibit or prevent the proliferation of NEOPLASMS.Major Histocompatibility Complex: The genetic region which contains the loci of genes which determine the structure of the serologically defined (SD) and lymphocyte-defined (LD) TRANSPLANTATION ANTIGENS, genes which control the structure of the IMMUNE RESPONSE-ASSOCIATED ANTIGENS, HUMAN; the IMMUNE RESPONSE GENES which control the ability of an animal to respond immunologically to antigenic stimuli, and genes which determine the structure and/or level of the first four components of complement.Histocompatibility Antigens Class I: Membrane glycoproteins consisting of an alpha subunit and a BETA 2-MICROGLOBULIN beta subunit. In humans, highly polymorphic genes on CHROMOSOME 6 encode the alpha subunits of class I antigens and play an important role in determining the serological specificity of the surface antigen. Class I antigens are found on most nucleated cells and are generally detected by their reactivity with alloantisera. These antigens are recognized during GRAFT REJECTION and restrict cell-mediated lysis of virus-infected cells.Histocompatibility Antigens Class II: Large, transmembrane, non-covalently linked glycoproteins (alpha and beta). Both chains can be polymorphic although there is more structural variation in the beta chains. The class II antigens in humans are called HLA-D ANTIGENS and are coded by a gene on chromosome 6. In mice, two genes named IA and IE on chromosome 17 code for the H-2 antigens. The antigens are found on B-lymphocytes, macrophages, epidermal cells, and sperm and are thought to mediate the competence of and cellular cooperation in the immune response. The term IA antigens used to refer only to the proteins encoded by the IA genes in the mouse, but is now used as a generic term for any class II histocompatibility antigen.Genes, MHC Class I: Genetic loci in the vertebrate major histocompatibility complex which encode polymorphic characteristics not related to immune responsiveness or complement activity, e.g., B loci (chicken), DLA (dog), GPLA (guinea pig), H-2 (mouse), RT-1 (rat), HLA-A, -B, and -C class I genes of man.H-2 Antigens: The major group of transplantation antigens in the mouse.Histocompatibility Antigens: A group of antigens that includes both the major and minor histocompatibility antigens. The former are genetically determined by the major histocompatibility complex. They determine tissue type for transplantation and cause allograft rejections. The latter are systems of allelic alloantigens that can cause weak transplant rejection.Antigens: Substances that are recognized by the immune system and induce an immune reaction.Genes, MHC Class II: Genetic loci in the vertebrate major histocompatibility complex that encode polymorphic products which control the immune response to specific antigens. The genes are found in the HLA-D region in humans and in the I region in mice.HLA Antigens: Antigens determined by leukocyte loci found on chromosome 6, the major histocompatibility loci in humans. They are polypeptides or glycoproteins found on most nucleated cells and platelets, determine tissue types for transplantation, and are associated with certain diseases.HLA-D Antigens: Human immune-response or Class II antigens found mainly, but not exclusively, on B-lymphocytes and produced from genes of the HLA-D locus. They are extremely polymorphic families of glycopeptides, each consisting of two chains, alpha and beta. This group of antigens includes the -DR, -DQ and -DP designations, of which HLA-DR is most studied; some of these glycoproteins are associated with certain diseases, possibly of immune etiology.Immunity, Maternally-Acquired: Resistance to a disease-causing agent induced by the introduction of maternal immunity into the fetus by transplacental transfer or into the neonate through colostrum and milk.Terminology as Topic: The terms, expressions, designations, or symbols used in a particular science, discipline, or specialized subject area.Bodily Secretions: Endogenous substances produced through the activity of intact cells of glands, tissues, or organs.Virus Diseases: A general term for diseases produced by viruses.Crowdsourcing: Social media model for enabling public involvement and recruitment in participation. Use of social media to collect feedback and recruit volunteer subjects.Hepatitis C: INFLAMMATION of the LIVER in humans caused by HEPATITIS C VIRUS, a single-stranded RNA virus. Its incubation period is 30-90 days. Hepatitis C is transmitted primarily by contaminated blood parenterally, and is often associated with transfusion and intravenous drug abuse. However, in a significant number of cases, the source of hepatitis C infection is unknown.Dictionaries as Topic: Lists of words, usually in alphabetical order, giving information about form, pronunciation, etymology, grammar, and meaning.Antibodies: Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).Antibodies, Monoclonal: Antibodies produced by a single clone of cells.Hepatitis B: INFLAMMATION of the LIVER in humans caused by a member of the ORTHOHEPADNAVIRUS genus, HEPATITIS B VIRUS. It is primarily transmitted by parenteral exposure, such as transfusion of contaminated blood or blood products, but can also be transmitted via sexual or intimate personal contact.Cell Hypoxia: A condition of decreased oxygen content at the cellular level.Prostatic Neoplasms: Tumors or cancer of the PROSTATE.Anoxia: Relatively complete absence of oxygen in one or more tissues.Mice, Nude: Mutant mice homozygous for the recessive gene "nude" which fail to develop a thymus. They are useful in tumor studies and studies on immune responses.Transplantation, Heterologous: Transplantation between animals of different species.Neoplasm Metastasis: The transfer of a neoplasm from one organ or part of the body to another remote from the primary site.Cell Line, Tumor: A cell line derived from cultured tumor cells.Prostate: A gland in males that surrounds the neck of the URINARY BLADDER and the URETHRA. It secretes a substance that liquefies coagulated semen. It is situated in the pelvic cavity behind the lower part of the PUBIC SYMPHYSIS, above the deep layer of the triangular ligament, and rests upon the RECTUM.Neoplasm Transplantation: Experimental transplantation of neoplasms in laboratory animals for research purposes.Oxygen: An element with atomic symbol O, atomic number 8, and atomic weight [15.99903; 15.99977]. It is the most abundant element on earth and essential for respiration.Space Flight: Travel beyond the earth's atmosphere.Immune Evasion: Methods used by pathogenic organisms to evade a host's immune system.Muromegalovirus: A genus of the family HERPESVIRIDAE, subfamily BETAHERPESVIRINAE, causing infection involving several organs in mice and rats. Murid herpesvirus is the type species.Cytomegalovirus: A genus of the family HERPESVIRIDAE, subfamily BETAHERPESVIRINAE, infecting the salivary glands, liver, spleen, lungs, eyes, and other organs, in which they produce characteristically enlarged cells with intranuclear inclusions. Infection with Cytomegalovirus is also seen as an opportunistic infection in AIDS.Cytomegalovirus Infections: Infection with CYTOMEGALOVIRUS, characterized by enlarged cells bearing intranuclear inclusions. Infection may be in almost any organ, but the salivary glands are the most common site in children, as are the lungs in adults.Herpesviridae Infections: Virus diseases caused by the HERPESVIRIDAE.Immune System: The body's defense mechanism against foreign organisms or substances and deviant native cells. It includes the humoral immune response and the cell-mediated response and consists of a complex of interrelated cellular, molecular, and genetic components.Tumor Escape: The ability of tumors to evade destruction by the IMMUNE SYSTEM. Theories concerning possible mechanisms by which this takes place involve both cellular immunity (IMMUNITY, CELLULAR) and humoral immunity (ANTIBODY FORMATION), and also costimulatory pathways related to CD28 antigens (ANTIGENS, CD28) and CD80 antigens (ANTIGENS, CD80).NK Cell Lectin-Like Receptor Subfamily A: An inhibitory subclass of NK cell lectin-like receptors that interacts with CLASS I MAJOR HISTOCOMPATIBILITY ANTIGENS and prevents the activation of NK CELLS.Membrane Microdomains: Detergent-insoluble CELL MEMBRANE components. They are enriched in SPHINGOLIPIDS and CHOLESTEROL and clustered with glycosyl-phosphatidylinositol (GPI)-anchored proteins.Galectin 2: A galectin found preferentially expressed in the gastrointestinal tract. The protein occurs as a homodimer with two 14-kDa subunits.Lymphocyte Specific Protein Tyrosine Kinase p56(lck): This enzyme is a lymphoid-specific src family tyrosine kinase that is critical for T-cell development and activation. Lck is associated with the cytoplasmic domains of CD4, CD8 and the beta-chain of the IL-2 receptor, and is thought to be involved in the earliest steps of TCR-mediated T-cell activation.Lipids: A generic term for fats and lipoids, the alcohol-ether-soluble constituents of protoplasm, which are insoluble in water. They comprise the fats, fatty oils, essential oils, waxes, phospholipids, glycolipids, sulfolipids, aminolipids, chromolipids (lipochromes), and fatty acids. (Grant & Hackh's Chemical Dictionary, 5th ed)Glycosphingolipids: Lipids containing at least one monosaccharide residue and either a sphingoid or a ceramide (CERAMIDES). They are subdivided into NEUTRAL GLYCOSPHINGOLIPIDS comprising monoglycosyl- and oligoglycosylsphingoids and monoglycosyl- and oligoglycosylceramides; and ACIDIC GLYCOSPHINGOLIPIDS which comprises sialosylglycosylsphingolipids (GANGLIOSIDES); SULFOGLYCOSPHINGOLIPIDS (formerly known as sulfatides), glycuronoglycosphingolipids, and phospho- and phosphonoglycosphingolipids. (From IUPAC's webpage)Sphingomyelins: A class of sphingolipids found largely in the brain and other nervous tissue. They contain phosphocholine or phosphoethanolamine as their polar head group so therefore are the only sphingolipids classified as PHOSPHOLIPIDS.Signal Transduction: The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.Antigens, CD3: Complex of at least five membrane-bound polypeptides in mature T-lymphocytes that are non-covalently associated with one another and with the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL). The CD3 complex includes the gamma, delta, epsilon, zeta, and eta chains (subunits). When antigen binds to the T-cell receptor, the CD3 complex transduces the activating signals to the cytoplasm of the T-cell. The CD3 gamma and delta chains (subunits) are separate from and not related to the gamma/delta chains of the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA).Antigens, CD8: Differentiation antigens found on thymocytes and on cytotoxic and suppressor T-lymphocytes. CD8 antigens are members of the immunoglobulin supergene family and are associative recognition elements in MHC (Major Histocompatibility Complex) Class I-restricted interactions.Allergy and Immunology: A medical specialty concerned with the hypersensitivity of the individual to foreign substances and protection from the resultant infection or disorder.Fatal Outcome: Death resulting from the presence of a disease in an individual, as shown by a single case report or a limited number of patients. This should be differentiated from DEATH, the physiological cessation of life and from MORTALITY, an epidemiological or statistical concept.European Continental Ancestry Group: Individuals whose ancestral origins are in the continent of Europe.Tomography, X-Ray Computed: Tomography using x-ray transmission and a computer algorithm to reconstruct the image.Lung: Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.Chemokine CCL1: A CC-type chemokine secreted by activated MONOCYTES and T-LYMPHOCYTES. It has specificity for CCR8 RECEPTORS.Organ Culture Techniques: A technique for maintenance or growth of animal organs in vitro. It refers to three-dimensional cultures of undisaggregated tissue retaining some or all of the histological features of the tissue in vivo. (Freshney, Culture of Animal Cells, 3d ed, p1)Culture Techniques: Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Tissue Culture Techniques: A technique for maintaining or growing TISSUE in vitro, usually by DIFFUSION, perifusion, or PERFUSION. The tissue is cultured directly after removal from the host without being dispersed for cell culture.Lung Diseases: Pathological processes involving any part of the LUNG.Cercopithecus: A genus of Old World monkeys found in Africa although some species have been introduced into the West Indies. This genus is composed of at least twenty species: C. AETHIOPS, C. ascanius, C. campbelli, C. cephus, C. denti, C. diana, C. dryas, C. erythrogaster, C. erythrotis, C. hamlyni, C. lhoesti, C. mitis, C. mona, C. neglectus, C. nictitans, C. petaurista, C. pogonias, C. preussi, C. salongo, and C. wolfi.Cercopithecus aethiops: A species of CERCOPITHECUS containing three subspecies: C. tantalus, C. pygerythrus, and C. sabeus. They are found in the forests and savannah of Africa. The African green monkey (C. pygerythrus) is the natural host of SIMIAN IMMUNODEFICIENCY VIRUS and is used in AIDS research.Erythrocebus patas: A species of the genus ERYTHROCEBUS, subfamily CERCOPITHECINAE, family CERCOPITHECIDAE. It inhabits the flat open arid country of Africa. It is also known as the patas monkey or the red monkey.Monkey Diseases: Diseases of Old World and New World monkeys. This term includes diseases of baboons but not of chimpanzees or gorillas (= APE DISEASES).Avian Sarcoma Viruses: Group of alpharetroviruses (ALPHARETROVIRUS) producing sarcomata and other tumors in chickens and other fowl and also in pigeons, ducks, and RATS.Haplorhini: A suborder of PRIMATES consisting of six families: CEBIDAE (some New World monkeys), ATELIDAE (some New World monkeys), CERCOPITHECIDAE (Old World monkeys), HYLOBATIDAE (gibbons and siamangs), CALLITRICHINAE (marmosets and tamarins), and HOMINIDAE (humans and great apes).Transformation, Genetic: Change brought about to an organisms genetic composition by unidirectional transfer (TRANSFECTION; TRANSDUCTION, GENETIC; CONJUGATION, GENETIC, etc.) and incorporation of foreign DNA into prokaryotic or eukaryotic cells by recombination of part or all of that DNA into the cell's genome.African Americans: Persons living in the United States having origins in any of the black groups of Africa.Rous sarcoma virus: A species of replication-competent oncogene-containing virus in the genus ALPHARETROVIRUS. It is the original source of the src oncogene (V-SRC GENES) and causes sarcoma in chickens.Cell Transformation, Viral: An inheritable change in cells manifested by changes in cell division and growth and alterations in cell surface properties. It is induced by infection with a transforming virus.Aralia: A plant genus in the family ARALIACEAE, order Apiales, subclass Rosidae. It includes Aralia californica S. Watson, Aralia nudicaulis L., and Aralia racemosa L.Arctium: A plant genus of the family ASTERACEAE. Arctiin (LIGNANS) is in the seed.Oleanolic Acid: A pentacyclic triterpene that occurs widely in many PLANTS as the free acid or the aglycone for many SAPONINS. It is biosynthesized from lupane. It can rearrange to the isomer, ursolic acid, or be oxidized to taraxasterol and amyrin.Saponins: A type of glycoside widely distributed in plants. Each consists of a sapogenin as the aglycone moiety, and a sugar. The sapogenin may be a steroid or a triterpene and the sugar may be glucose, galactose, a pentose, or a methylpentose.Biological Science Disciplines: All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.PakistanTriterpenesNitric Oxide: A free radical gas produced endogenously by a variety of mammalian cells, synthesized from ARGININE by NITRIC OXIDE SYNTHASE. Nitric oxide is one of the ENDOTHELIUM-DEPENDENT RELAXING FACTORS released by the vascular endothelium and mediates VASODILATION. It also inhibits platelet aggregation, induces disaggregation of aggregated platelets, and inhibits platelet adhesion to the vascular endothelium. Nitric oxide activates cytosolic GUANYLATE CYCLASE and thus elevates intracellular levels of CYCLIC GMP.Gastrodia: A plant genus of the family ORCHIDACEAE which depends on the fungus Armillaria mellea to complete its life cycle. It is an ingredient of Zhenxuanyin (DRUGS, CHINESE HERBAL).Biology: One of the BIOLOGICAL SCIENCE DISCIPLINES concerned with the origin, structure, development, growth, function, genetics, and reproduction of animals, plants, and microorganisms.
(1/424) A different intracellular distribution of a single reporter protein is determined at steady state by KKXX or KDEL retrieval signals.

To establish the specific contribution to protein topology of KKXX and KDEL retrieval motifs, we have determined by immunogold electron microscopy and cell fractionation the intracellular distribution at steady state of the transmembrane and anchorless versions of human CD8 protein, tagged with KKXX (CD8-E19) and KDEL (CD8-K), respectively, and stably expressed in epithelial rat cells (Martire, G., Mottola, G., Pascale, M. C., Malagolini, N., Turrini, I., Serafini-Cessi, F., Jackson, M. R., and Bonatti, S. (1996) J. Biol. Chem. 271, 3541-3547). The CD8-E19 protein is represented by a single form, initially O-glycosylated: only about half of it is located in the endoplasmic reticulum, whereas more than 30% of the total is present in the intermediate compartment and cis-Golgi complex. In the latter compartments, CD8-E19 colocalizes with beta-coat protein (COP) (COPI component) and shows the higher density of labeling. Conversely, about 90% of the total CD8-KDEL protein is localized in clusters on the endoplasmic reticulum, where significant co-localization with Sec-23p (COPII component) is observed, and unglycosylated and initially O-glycosylated forms apparently constitute a single pool. Altogether, these results suggest that KKXX and KDEL retrieval motifs have different topological effects on theirs own at steady state: the first results in a specific enrichment in the intermediate compartment and cis-Golgi complex, and the latter dictates residency in the endoplasmic reticulum.  (+info)

(2/424) The adenovirus type 5 E1b 55K and E4 Orf3 proteins associate in infected cells and affect ND10 components.

Three early proteins expressed by adenovirus type 5, E1b 55K, E4 Orf3 and E4 Orf6, are involved in regulating late viral gene expression. It has previously been shown that 55K associates with Orf6. Here we show that 55K also associates with Orf3 and that this interaction is necessary for 55K to localize to the nuclear matrix fraction of the cell. From our data, we infer that the Orf3 and Orf6 interactions with 55K may be mutually exclusive. The Orf3 protein is also known to associate with and cause the reorganization of cell nucleus structures known as ND10 or PODs. Consistent with the observed increase in the biochemical interaction between 55K and Orf3 in the absence of Orf6, the 55K association with Orf3 in ND10 was also found to increase in the absence of Orf6. The most studied cellular component of ND10 is PML, a complex protein present in a range of isoforms, some of which are modified by conjugation to the small ubiquitin-like protein PIC-1. The pattern of PML isoforms was altered in adenovirus-infected cells, in that a number of additional isoform bands appeared in an Orf3-dependent manner, one of which became predominant later in infection. As for ND10 reorganization, neither Orf6 nor 55K was required for this effect. Therefore it is likely that these changes in PML are related to the changes in ND10 structure that occur during infection.  (+info)

(3/424) Reduced toxicity, attenuated immunogenicity and efficient mediation of human p53 gene expression in vivo by an adenovirus vector with deleted E1-E3 and inactivated E4 by GAL4-TATA promoter replacement.

A recombinant adenovirus with deleted E1 and E3, and E4-inactivated by replacing the E4 promoter with a synthetic promoter composed of a minimal TATA box and five consensus yeast GAL4-binding site elements was developed and used to express the human tumor suppresser gene p53. The toxicity and immunogenicity of this vector and vector-mediated p53 gene expression in vivo were studied in immunocompetent C3H and C57BL/6 mice. Expression of the late viral gene product, hexon protein, was observed in C3H and C57BL/6 mice injected with E4 wild-type adenovirus constructs Adv-cmv-beta-Gal (BG), Adv-cmv-hp53 (WT), and empty E1- vector Adv-E4 (EW) 3 to 28 days after injection, but was undetectable in mice treated with E4 modified empty E1- vector Adv-GAL4 (EG) or Adv-cmv-hp53-GAL4 (G4). Expression of the p53 gene was observed in both WT- and G4-injected C3H and C57BL/6 mouse livers from days 3 to 28. Ten weeks after injection, p53 gene expression was still detected in G4-treated C57BL/6 mice at similar levels, but was not detectable in WT-treated mice. Vector-induced liver toxicity was evaluated by analyzing serum transaminases (SGOT and SGPT) activities. In all cases, SGOT and SGPT activities were markedly decreased in EG-treated C3H and C57BL/6 mice compared with those in EW-treated mice on days 3, 7 and 14 after injection. In C57BL/6 mice, the total anti-adenoviral CTL activities were two- to three-fold higher in animals treated with EW vector than in those treated with EG vector. These results suggest that inactivation of the E4 promoter efficiently diminished the viral replication and the late viral gene expression, reduced host immune response and consequently reduced toxicity and prolonged the duration of transgene expression in vivo.  (+info)

(4/424) Rapid construction of adenoviral vectors by lambda phage genetics.

Continued improvements of adenoviral vectors require the investigation of novel genome configurations. Since adenovirus can be generated directly by transfecting packaging cell lines with viral genomes isolated from plasmid DNA, it is possible to separate genome construction from virus production. In this way failure to generate a virus is not associated with an inability to generate the desired genome. We have developed a novel lambda-based system that allows rapid modification of the viral genome by double homologous recombination in Escherichia coli. The recombination reaction and newly generated genome may reside in a recombination-deficient bacterial host for enhanced plasmid stability. Furthermore, the process is independent of any restriction endonucleases. The strategy relies on four main steps: (i) homologous recombination between an adenovirus cosmid and a donor plasmid (the donor plasmid carries the desired modification[s] and flanking regions of homology to direct its recombination into the viral genome); (ii) in vivo packaging of the recombinant adenoviral cosmids during a productive lambda infection; (iii) transducing a recombination-deficient E. coli lambda lysogen with the generated lysate (the lysogen inhibits the helper phage used to package the recombinant andenoviral cosmid from productively infecting and destroying the host bacteria); (iv) effectively selecting for the desired double-recombinant cosmid. Approximately 10,000 double-recombinant cosmids are recovered per reaction with essentially all of them being the correct double-recombinant molecule. This system was used to generate quickly and efficiently adenoviral genomes deficient in the E1/E3 and E1/E3/E4 regions. The basis of this technology allows any region of the viral genome to be readily modified for investigation of novel configurations.  (+info)

(5/424) Unique features of fowl adenovirus 9 gene transcription.

We examined the transcriptional organization of fowl adenovirus 9 (FAdV-9) and analyzed temporal transcription profiles of its early and late mRNAs. At least six early and six late transcriptional regions were identified for FAdV-9. Extensive splicing was observed in all FAdV-9 early transcripts examined. Sequence analysis of the cDNAs representing the early proteins identified untranslated leader sequences, precise locations of splice donor and acceptor sites, as well as polyadenylation signals and polyadenylation sites. A unique characteristic, compared to other adenoviruses, was the detection by RT-PCR of multiple transcripts specific for each of five late genes (protein III, pVII, pX, 100K, and fiber), suggesting that FAdV-9 late transcripts undergo more extensive splicing than reported for other adenoviruses.  (+info)

(6/424) Activation of adenovirus early promoters and lytic phase in differentiated strata of organotypic cultures of human keratinocytes.

Human oncolytic adenoviruses have been used in clinical trials targeting cancers of epithelial origin. To gain a better understanding of the infectious cycle of adenovirus in normal human squamous tissues, we examined the viral infection process in organotypic cultures of primary human keratinocytes. We show that for the infection to occur, wounding of the epithelium is required. In addition, infection appears to initiate at the basal or parabasal cells that express the high-affinity coxsackievirus-adenovirus receptor, CAR, whereas the productive phase takes place in differentiated cells. This is due, at least in part, to the differentiation-dependent activation of the E1A and E2A early promoters and E4 promoters. We also show that adenovirus infection triggers a response mediated by the abnormal accumulation of cyclin E and p21cip1 proteins similar to the one previously observed in human papillomavirus-infected tissues. However, the virus seems to be able to overcome it, at least partially.  (+info)

(7/424) NF-IL6, a member of the C/EBP family, regulates E1A-responsive promoters in the absence of E1A.

A cDNA encoding NF-IL6, an interleukin-6 (IL-6)-regulated human nuclear factor of the C/EBP family, is demonstrated to complement the transactivation function of E1A. The endogenous NF-IL6 level varies according to cell type and correlates positively with an IL-6-regulated cellular E1A-substituting activity that was described recently (J.M. Spergel and S. Chen-Kiang, Proc. Natl. Acad. Sci. USA 88:6472-6476, 1991). When expressed by transfection in cells which contain low levels of NF-IL6 and are incapable of complementing the function of E1A proteins, NF-IL6 also transactivates the E1A-responsive E2ae and E1B promoters, to the same magnitude as E1A. Activation by NF-IL6 is concentration dependent and sequence specific: mutational studies of the E2ae promoter suggest that the promoter-proximal NF-IL6 recognition site functions as a dominant negative regulatory site whereas the promoter-distal NF-IL6 recognition site is positively regulated at low NF-IL6 concentrations and negatively regulated when the NF-IL6 level is high. Consistent with these functions, NF-IL6 alone is sufficient to complement an E1A deletion mutant dl312 in viral infection, when expressed at appropriate concentrations. These results identify NF-IL6 as a sequence-specific cellular nuclear factor which regulates E1A-responsive genes in the absence of E1A.  (+info)

(8/424) Adenovirus E1A represses the cyclic AMP-induced transcription of the gene for phosphoenolpyruvate carboxykinase (GTP) in hepatoma cells.

Adenovirus infection of hepatoma cells inhibited transcription of the phosphoenolpyruvate carboxykinase (GTP) (EC 4.1.1.32) (PEPCK) gene and virtually eliminated transcription of a chimeric gene which contained the PEPCK promoter linked to the structural gene for chloramphenicol acetyltransferase (CAT). This effect is due to the viral protein E1A, since adenovirus containing a deletion in the E1A gene did not repress transcription from the PEPCK promoter. Both the 243R and 283R products of the E1A gene were effective. The conserved region 1 (CR-1) domain of E1A was required for this effect. Treatment of hepatoma cells with 8-bromo-cAMP or transfection with plasmids coding for the catalytic subunit of protein kinase A, CAAT/enhancer binding protein alpha (C/EBP), or Jun, all potent inducers of PEPCK gene transcription, did not relieve the inhibition caused by E1A. This inhibition does not appear to be mediated by major enhancer elements and in the PEPCK gene since transcription from the PEPCK promoter containing block mutations in binding domains for C/EBP and cAMP regulatory element binding protein (CREB) was also inhibited by E1A. Transcription of chimeric genes containing two copies each of the major cAMP response domains (CRE-1 and P-3) linked to a neutral promoter and fused to the CAT structural gene was stimulated by the catalytic subunit of protein kinase A, but this effect was totally inhibited by E1A. The strong repressive effect of E1A on PEPCK gene transcription seems to involve an interruption of an obligatory interaction between factors which bind to the cAMP response element in the PEPCK promoter and the TATA box.  (+info)

*  Adenovirus genome
The functions of many adenovirus proteins are known: Structural proteins include capsid proteins II (hexon), III (penton base ... The E1A, E1B, E2A, E2B, E3, and E4 transcription units are successively transcribed early in the viral reproductive cycle. The ... Control protein E1B 19K suppresses apoptosis by mimicking the action of cellular protein Bcl-2. Control protein E1B 55K binds ... "Protein Details for Human adenovirus E". NCBI. Retrieved 2013-01-17. Russell, WC (Jan 2009). "Adenoviruses: update on structure ...
*  Oncolytic adenovirus
Yew, P.; Berk, A. (1992). "Inhibition of p53 transactivation required for transformation by adenovirus early 1B protein". ... and the knob domain of the adenovirus coat protein trimer. CAR is necessary for adenovirus infection. Although expressed widely ... For adenovirus replication to occur, the host cell must be induced into S phase by viral proteins interfering with cell cycle ... E1B proteins produced by adenoviruses protect the infected cell by binding to and degrading the p53 transcription factors, ...
*  List of MeSH codes (D12.776)
... oncogene protein tpr-met MeSH D12.776.624.664.520.045 - adenovirus early proteins MeSH D12.776.624.664.520.045.050 - adenovirus ... adenovirus e2 proteins MeSH D12.776.624.664.520.045.070 - adenovirus e3 proteins MeSH D12.776.624.664.520.045.080 - adenovirus ... adenovirus e1a proteins MeSH D12.776.964.700.045.050.110 - adenovirus e1b proteins MeSH D12.776.964.700.045.060 - adenovirus e2 ... adenovirus e3 proteins MeSH D12.776.964.700.045.080 - adenovirus e4 proteins MeSH D12.776.964.700.750.320 - fusion proteins, ...
*  Adenovirus early region 1A
... proteins. It has been considered an oncogene. It can behave as a tumour suppressor gene. "Introduction To Ad5 E1A". "Adenovirus ... Adenovirus early region 1A (E1a or E1A) is a gene expressed during adenovirus replication to produce a variety of (E1A) ... The Adenovirus 5 E1A page Molecular Genetics of the Adenovirus E1A Oncoproteins P03255 (E1A_ADE05). ... ". "Adenovirus-5 E1A: paradox and paradigm". Nat Rev Mol Cell Biol. 3 (6): 441-52. Jun 2002. doi:10.1038/nrm827. PMID 12042766 ...
*  EP300
Histone acetyltransferase p300 also known as p300 HAT or E1A-associated protein p300 (where E1A = adenovirus early region 1A) ... "Molecular cloning and functional analysis of the adenovirus E1A-associated 300-kD protein (p300) reveals a protein with ... "cAMP-response-element-binding-protein-binding protein (CBP) and p300 are transcriptional co-activators of early growth response ... This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. EP300 is closely related to ...
*  List of MeSH codes (D23)
... adenovirus early proteins MeSH D23.050.327.045.050 --- adenovirus e1 proteins MeSH D23.050.327.045.060 --- adenovirus e2 ... proteins MeSH D23.050.327.045.070 --- adenovirus e3 proteins MeSH D23.050.327.045.080 --- adenovirus e4 proteins MeSH D23.050. ... adenovirus e1a proteins MeSH D23.050.285.062.050 --- adenovirus e1b proteins MeSH D23.050.285.062.090 --- antigens, ... adenovirus e1a proteins MeSH D23.050.327.062.050 --- adenovirus e1b proteins MeSH D23.050.327.062.090 --- antigens, ...
*  Kumaravel Somasundaram
... regulating the protein, p73 in cervical cancer patients, using Adenovirus early region 1A, a tumor suppressor gene, the ... The research team led by Somasundaram and Phillipe Marin of INSERM validated the earlier findings and proposed a new ... Das, Sanjeev; Nama, Srikanth; Antony, Sini; Somasundaram, Kumaravel (2005). "p73β-expressing recombinant adenovirus: a ... "IGF2 mRNA binding protein 3 (IMP3) promotes glioma cell migration by enhancing the translation of RELA/p65". Oncotarget. 8 (25 ...
*  Adenoviridae
The early genes are responsible for expressing mainly non-structural, regulatory proteins. The goal of these proteins is ... Adenoviruses Stanford University - Adenoviruses Adenoviruses General Concepts General information on Adenovirus DNA virus ... DNA replication separates the early and late phases. Once the early genes have liberated adequate virus proteins, replication ... Tupaia adenovirus (TAV) (Tree shrew adenovirus 1) has been isolated from tree shrews. Otarine adenovirus 1 has been isolated ...
*  Secreted frizzled-related protein 1
Using adenovirus-expressing SFRP1, impaired the canonical Wnt/Fzd pathway in the early phase of ischemia and as a result ... the SFRP1 netrin-related motif is also found in a range of other proteins that is thought to mediate protein-protein ... Loss of SFRP1 protein expression is associated with poor overall survival (OS) in patients with early breast cancer (pT1 ... Secreted frizzled-related protein 1, also known as SFRP1, is a protein which in humans is encoded by the SFRP1 gene. Secreted ...
*  Svante Pääbo
He earned his PhD from Uppsala University in 1986 for research investigating how the E19 protein of adenoviruses modulates the ... Pääbo is known as one of the founders of paleogenetics, a discipline that uses the methods of genetics to study early humans ... How the E19 protein of adenoviruses modulates the immune system (PhD thesis). Uppsala University. ISBN 9155419216. OCLC ...
*  Oncovirus
The adenovirus E1B protein (55K) prevents p53 from regulating genes by binding to the site on p53 which binds to the genome. In ... The adenovirus early region 1A (E1A) is an oncoprotein which binds to Rb and can stimulate transcription and transform cells. ... HPV instead degrades p53: the HPV protein E6 binds to a cellular protein called the E6-associated protein (E6-AP, also known as ... DNA oncoviruses typically impair two families of tumor suppressor proteins: tumor proteins p53 and the retinoblastoma proteins ...
*  E2F
pRb is one of the targets of the oncogenic human papilloma virus protein E7, and human adenovirus protein E1A. By binding to ... footprinting experiments obtained on Cdc2 and B-myb promoters demonstrated E2F DNA binding site occupation during G0 and early ... Most E2F have a pocket protein binding domain. Pocket proteins such as pRB and related proteins p107 and p130, can bind to E2F ... Homo sapiens E2F1 mRNA or E2F1 protein sequences from NCBI protein and nucleotide database. X-ray crystallographic analysis has ...
*  VA RNA
These two VA RNA genes are distinct genes in the adenovirus genome. VA RNAI is the major species with VA RNAII expressed at a ... VAI stimulates the translation of both early and late viral genes including E3 and hexon. VAII does not stimulate translation. ... VAI RNA functions as a decoy RNA for the double stranded RNA activated protein kinase R which would otherwise phosphorylate ... The VA (viral associated) RNA is a type of non-coding RNA found in adenovirus. It plays a role in regulating translation. There ...
*  History of genetics
... utilizing a form of Adenovirus to carry a replacement gene coding for the p53 protein. 2016: A genome is sequenced in outer ... in 1986 by Walter Gilbert that neither DNA nor protein would be required in such a primitive system as that of a very early ... "protein foreign to a unicellular organism." These two scientist were able to replicate proteins such as HGH, Erythropoietin and ... The most influential early theories of heredity were that of Hippocrates and Aristotle. Hippocrates' theory (possibly based on ...
*  Adenovirus E1B protein
"Adenovirus early region 1B 58,000-dalton tumor antigen is physically associated with an early region 4 25,000-dalton protein in ... Adenovirus E1B protein usually refers to one of two proteins transcribed from the E1B gene of the adenovirus: a 55kDa protein ... These two proteins are needed to block apoptosis in adenovirus-infected cells. E1B proteins work to prevent apoptosis that is ... White, E; Cipriani, R (January 10, 1990). "Role of adenovirus E1B proteins in transformation: altered organization of ...
*  GOLM1
Puri S, Bachert C, Fimmel CJ, Linstedt AD (2003). "Cycling of early Golgi proteins via the cell surface and endosomes upon ... Kladney RD, Tollefson AE, Wold WS, Fimmel CJ (2002). "Upregulation of the Golgi protein GP73 by adenovirus infection requires ... Golgi membrane protein 1 (GOLM1) also known as Golgi phosphoprotein 2 or Golgi membrane protein GP73 is a protein that in ... The protein encoded by this gene is a type II Golgi transmembrane protein. It processes protein synthesized in the rough ...
*  Alternative splicing
The Adenovirus produces five primary transcripts early in its infectious cycle, prior to viral DNA replication, and an ... Most of the activator proteins that bind to ISEs and ESEs are members of the SR protein family. Such proteins contain RNA ... When the RNA attached to that protein is isolated and cloned, it reveals the target sequences for that protein. Another method ... Females produce the master sex determination protein Sex lethal (Sxl). The Sxl protein is a splicing repressor that binds to an ...
*  Experimental cancer treatment
Adenoviruses are a commonly utilized vector for this purpose. Much research has focused on the use of adenoviruses that cannot ... As p53 protein levels are usually kept low, one could block its degradation and allow large amounts of p53 to accumulate, thus ... Early clinical trials typically enroll a very small number of patients, and the purpose is to identify major safety issues and ... On average, 95% of the participants in these early trials receive no benefit, but all are exposed to the risk of adverse ...
*  Maurice Green (virologist)
He discovered two of the proteins required for adenoviruses to transform cells, and also other proteins that usurp the infected ... They also emerged as a vehicle for human gene therapy Also in the early 1960s, Green and others showed that human adenoviruses ... Lillie, J. W.; Loewenstein, P. M.; Green, M. R.; Green, M. (1987). "Functional domains of adenovirus type 5 E1a proteins". Cell ... Virology, 428 Loewenstein, P. M.; Green, M. (2011). "Expression of the Adenovirus Early Gene 1A Transcription-Repression Domain ...
*  Coxsackie virus and adenovirus receptor
Coxsackievirus and adenovirus receptor (CAR) is a protein that in humans is encoded by the CXADR gene. The protein encoded by ... "Coxsackievirus-adenovirus receptor (CAR) is essential for early embryonic cardiac development". Journal of Cell Science. 118 ( ... "Entrez Gene: CXADR coxsackie virus and adenovirus receptor". "Protein sequence of human CXADR (Uniprot ID: P78310)". Cardiac ... Law LK, Davidson BL (Jan 2002). "Adenovirus serotype 30 fiber does not mediate transduction via the coxsackie-adenovirus ...
*  Oncolytic herpes virus
A vital part of the normal mechanism of HSV-1, the ICP34.5 protein has been proposed to condition post-mitotic cells for viral ... The earlier and greater expression of US11 (also involved in overcoming PKR-mediated responses) largely overcomes the reduction ... Clinical trials Including phase III : See Talimogene laherparepvec Virotherapy Oncolytic virus Oncolytic adenovirus Herpes ... It also has a deletion of the gene encoding ICP47, a protein that inhibits antigen presentation, and an insertion of a gene ...
*  Viral vector
Protein coding genes can be expressed using viral vectors, commonly to study the function of the particular protein. Viral ... "Impact of Preexisting Adenovirus Vector Immunity on Immunogenicity and Protection Conferred with an Adenovirus-Based H5N1 ... In one of the early gene therapy trials in 1999 this led to the death of Jesse Gelsinger, who was treated using an adenoviral ... Adenoviruses are being actively developed as vaccines. Retroviruses are one of the mainstays of current gene therapy approaches ...
*  Retinoblastoma-like protein 2
Li Y, Graham C, Lacy S, Duncan AM, Whyte P (Dec 1993). "The adenovirus E1A-associated 130-kD protein is encoded by a member of ... are expressed in the early phases of the cell cycle". Proceedings of the National Academy of Sciences of the United States of ... Retinoblastoma-like protein 2 is a protein that in humans is encoded by the RBL2 gene. Retinoblastoma-like protein 2 has been ... Li Y, Graham C, Lacy S, Duncan AM, Whyte P (Dec 1993). "The adenovirus E1A-associated 130-kD protein is encoded by a member of ...
*  Mastadenovirus
Transcription of early genes (E genes) by host RNA pol II: these proteins optimize the cellular milieu for viral replication, ... serotype 14: can cause potentially fatal adenovirus infections. Canine adenovirus 1 (CAdV-1) can lead to death in puppies, or ... Expression of L4-22K and L4-33K causes early to late switch. Transcription of late genes (L genes) by host RNA pol II, mostly ... The genome codes for 40 proteins. Viral replication is nuclear. Entry into the host cell is achieved by attachment of the viral ...
*  CTDP1
"C-terminal domain phosphatase sensitivity of RNA polymerase II in early elongation complexes on the HIV-1 and adenovirus 2 ... The protein may also represent a component of an RNA polymerase II holoenzyme complex. Alternative splicing of this gene ... 2002). "A protein phosphatase from human T cells augments tat transactivation of the human immunodeficiency virus type 1 long- ... 1999). "A protein phosphatase functions to recycle RNA polymerase II". Genes Dev. 13 (12): 1540-52. doi:10.1101/gad.13.12.1540 ...
*  Microtubule
There are many proteins that bind to microtubules, including the motor proteins kinesin and dynein, severing proteins like ... Very low levels of free calcium can destabilize microtubules and this prevented early researchers from studying the polymer in ... Some viruses (including retroviruses, herpesviruses, parvoviruses, and adenoviruses) that require access to the nucleus to ... MAP-1 proteins consists of a set of three different proteins: A, B and C. The C protein plays an important role in the ...
Adenovirus Early Proteins: Proteins encoded by adenoviruses that are synthesized prior to, and in the absence of, viral DNA replication. The proteins are involved in both positive and negative regulation of expression in viral and cellular genes, and also affect the stability of viral mRNA. Some are also involved in oncogenic transformation.
Get an answer for Calculate Kc for the system, Ni2+ + Co Ni + Co2+. at 25 C?Ni2+ (aq) + 2e === Ni (s) E = - 0.25 V Co2+ (aq) + 2e === Co (s) E = - 0.28 and find homework help for other Science questions at eNotes
Encontre Livros em Inglês e outros idiomas de Ciências Biológicas e Zoologia. O maior acervo de livros em inglês e outros idiomas de ciências biológicas e os melhores autores você encontra aqui na Saraiva.
Encontre Livros em Inglês e outros idiomas de Gastronomia e Dietas e Regimes. O maior acervo de livros em inglês e outros idiomas de gastronomia e os melhores autores você encontra aqui na Saraiva.
Plasmid VV209: sdChR(C138S E154A)-TS-GCaMP6f-ER in fck from Dr. Adam Cohens lab contains the insert sdChR(C138S, E154A)-TS-GCaMP6f-ER and is published in Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020. This plasmid is available through Addgene.
Plasmid VV228: sdChR(C138S E154A)-TS in fck from Dr. Adam Cohens lab contains the insert sdChR(C138S, E154A)-TS and is published in Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020. This plasmid is available through Addgene.
+ Todays AvWeek stories Pacific Pivot Could Get More Congressional Attention Northrop Grumman Reveals Bigger Firebird Engine Tech, Cyber-Espionage Key To Chinas Progress + Acquisition Reform Pentagon Aims to Reduce Time, Cost for Weapons Design Defense Acquisition Chief: There Are No Simple Solutions Pentagon Launches Latest E
Tampereen kaupungin tietohallintojohtaja Jarkko Oksala johtaa rakennustyömaata, jossa tietohallinnon perustukset eivät rakoile kuntarajojen takia. Yhteisellä tontilla edetään valtakunnallisia kunta- ja sotelinjauksia odottelematta.
Definition of Adenovirus E3 10.4K/14.5kD Protein in the Legal Dictionary - by Free online English dictionary and encyclopedia. What is Adenovirus E3 10.4K/14.5kD Protein? Meaning of Adenovirus E3 10.4K/14.5kD Protein as a legal term. What does Adenovirus E3 10.4K/14.5kD Protein mean in law?
Gentaur molecular products has all kinds of products like :search , bio-gentaur \ VIROLOGY Adenovirus Type 5 Culture Fluid \ GEN0810020CF for more molecular products just contact us
Gentaur molecular products has all kinds of products like :search , bio-gentaur \ Adenovirus Type 14 Culture Fluid \ GEN0810108CF for more molecular products just contact us
Reaktivität: Fledermaus, Rind (Kuh), Hund and more. 114 verschiedene BNIP3L Antikörper vergleichen. Alle direkt auf antikörper-online bestellbar!
Adenovirus Type 9, 0.1 mg. The many different serotypes of human adenoviruses (Ad) are divided into six subgroups, of which all Ad subgroup A and B and two subgroup D Ads can elicit tumors in infected rodents.
Buy L3 antigen, ADENOVIRUS TYPE 5 Antigen-AP_000211.1 (MBS239153) product datasheet at MyBioSource, Antigens. Application: ELISA (EIA)
The early region (E1) of the adenovirus genome, responsible for transforming activity, is localized within the left most 11% of the viral genome and…
So I am new to the area and am beginning to think the state was misnamed...when do the yellow dust clouds stop blowing? I can wash the cars and an hour later e
... Early Region - corsi di forex online, forex study ru, software forex trading testing, best forex brokers in the uk, nord forex trading
RNA molecules from nuclear and cytoplasmic polyribosomes of adenovirus-infected HeLa cells were compared by hybridization to analyse the sequence content. Nuclear polyribosomes were released by exposure of intact detergent-washed nuclei to poly(U) and purified. Cytoplasmic polyribosomes were also purified from the same cells. To show that nuclear polyribosomes contain ribosomes linked by mRNA, polyribosomes were labelled with methionine and uridine in the presence of actinomycin D in adenovirus-infected cells. Purified nuclear polyribosomes were treated with EDTA under conditions which dissociate polyribosomes into ribosomes and subunits with a simultaneous release of mRNA, and sedimented. The treatment dissociated these polyribosomes, releasing the mRNA from them. Radiolabelled total RNA from each polyribosome population was fractionated in sucrose gradients into several pools or hybridized to intact adenovirus DNA to select virus-specific RNA. Sucrose-gradient-fractionated pool-3 RNA (about ...
Five distinct localization patterns were observed for the adenovirus E1A proteins in the nuclei of infected HeLa cells: diffuse, reticular, nucleolar, punctate, and peripheral. The variable distribution of E1A was correlated with the time postinfection and the cell cycle stage of the host cell at the time of infection. All staining patterns, with the exception of peripheral E1A localization, were associated with the early phase of infection since only the diffuse, reticular, nucleolar, and punctate staining patterns were observed in the presence of hydroxyurea. Because the E1A proteins (12S and 13S) stimulate the expression of the cellular heat shock 70-kilodalton protein (hsp70), we examined the intracellular distribution of hsp70 in the adenovirus-infected cells. Whereas hsp70 was predominantly cytoplasmic in the cells before infection, after adenovirus infection most of the protein was now found within the nucleus. Specifically, hsp70 was found within the nucleoli as well as exhibiting ...
Full custom adenovirus production services, adenovirus vector construction, adenovirus packaging, adenovirus amplification, adenovirus purification, adenovirus titering.
Our study is the first report of an RSAd, in which the "promoter-based regulation of E1A," approach is used to target the deregulated G1 to S phase in tumor cells. We demonstrated that AdE2F-1RC replicated selectively in tumor cells and not in normal cells expressing high and low levels of E2F-1 protein, respectively. Additionally, in two mouse xenograft models, AdE2F-1RC exhibited significant in vivo therapeutic benefit often equivalent to wild-type adenovirus treatment. These studies validate several design features of AdE2F-1RC.. The wild-type adenovirus dl309 replicated in all of the normal cells tested. We reasoned that normal resting cells would be a good model for AdE2F-1RC toxicity tests, because these cells do not express E2F-1 (44 , 45) and are found in the tumor environment. In contrast to dl309, the replication and CPE of AdE2F-1RC was significantly attenuated in normal cells suggesting that the E2F-1 promoter was not optimally activated. One reason is that the presence of pRb/E2F-1 ...
This post is about adenovirus infection, a major cause of illness both minor and severe in the United States, especially among children in group settings.
101721 - Circunferência escrotal e medidas morfométricas em cordeiros da raça Santa Inês e F1 Santa Inês X Dorper - Scrotal circumference and morphometric measurements in lambs Santa Inês and F1 Dorper x Santa Inês | Veterinaria.org . La primera comunidad veterinaria de habla hispana con presencia en Espa a y Am rica del Sur.
â€"·Â s‚ÌŽ‘Ši‚ðŽæ‚éÆ ÆÅ ÆbÆg Ť c‚à‚ ‚é‘  ‡â€"·Â s‹Æâ€"± â€"Æ ÓŽâ€" € e‚¨â€"₢ ‡‚킹 â€"·Â s‚ÌŽ‘Ši eŽíâ€"ނƎ擾•ûâ€"@ Page Ť c‚È‚Ç‚ ...
Find more information on adenovirus vector including adenovirus infection and adenovirus symptoms in Kidspots comprehensive health section.
BACKGROUND: Natural killer (NK) cells localize in the microcirculation in antibody-mediated rejection (AMR) and have been postulated to be activated by donor-specific anti-HLA antibodies triggering their CD16a Fc receptors. However, direct evidence for NK cell CD16a triggering in AMR is lacking. We hypothesized that CD16a-inducible NK cell-selective transcripts would be expressed in human AMR biopsies and would offer evidence for CD16a triggering. METHODS: We stimulated human NK cells through CD16a in vitro, characterized CD16a-inducible transcripts, and studied their expression in human kidney transplant biopsies with AMR and in an extended human cell panel to determine their selectivity ...
Jimenez-Garcia, L. F., Spector, D. L. (1992) Reorganization of the Pre-Messenger-Rna Splicing Apparatus Upon Adenovirus Infection. Molecular Biology of the Cell, 3. A322-A322. ISSN 1059-1524 ...
Purify adenovirus quickly and easily with our Adeno-X Maxi Purification Kits! These kits are chromatography-based systems that enable you to purify amplified adenovirus directly from the cell pellet in less than 1.5 hours.
AMSBIO offers a collection of adenovirus clones with more than 20 different destination vectors available for fluorescent and affinity tags.
Quick Tips content is self-published by the Dell Support Professionals who resolve issues daily. In order to achieve a speedy publication, Quick Tips may represent only partial solutions or work-arounds that are still in development or pending further proof of successfully resolving an issue. As such Quick Tips have not been reviewed, validated or approved by Dell and should be used with appropriate caution. Dell shall not be liable for any loss, including but not limited to loss of data, loss of profit or loss of revenue, which customers may incur by following any procedure or advice set out in the Quick Tips ...
U n i S e t . _ _ i n i t _ _ def __init__(self, node): Constructor. self.name = node.attrib[NAME_A] self.fpi = node.attrib.get(FPI_A, None ...
The DIGAMI-2 trial74 attempted to study 3 alternative treatment regimens: acute insulin-glucose infusion followed by insulin-based long-term glucose control; insulin-glucose infusion followed by standard glucose control on discharge; and routine metabolic management in both inpatient and outpatient settings. Although there were no differences in outcomes among the 1253 randomized AMI patients, this may be attributable to the similar short-term glucose control and identical longer-term glucose control obtained among the 3 groups. Most importantly, the longer-term fasting glucose target of 90 to 126 mg/dL was never achieved in the intensive-treatment group. Thus, despite its intent, DIGAMI-2 ended up comparing different insulin-treatment strategies, not different intensities of glucose control. Furthermore, like the original DIGAMI trial, DIGAMI-2 did not include any hyperglycemic patients without previously known diabetes, the group with the highest risk of glucose-associated death.. The HI-5 ...
Looking for Dog adenovirus? Find out information about Dog adenovirus. acute viral disease of canines, especially dogs and foxes. The causative agent, an adenovirus, is not infectious to humans. In foxes the disease is... Explanation of Dog adenovirus
There are many stages in the development of a new drug for viral infection and such processes are even further complicated for adenovirus by the fact that there are at least 51 serotypes, forming six distinct groups (A-F), with different degree of infectivity. This review attempts to address the importance of developing pharmaceuticals for adenovirus and also review recent development in drug discovery for adenovirus, including newer strategies such as microRNA approaches. Different drug screening strategies will also be discussed.
Adenoviruses commonly infect humans, causing colds, flu-like symptoms and sometimes even death, but now UC San Francisco researchers have discovered that a new species of adenovirus can spread from primate to primate, and ...
[205 Pages Report] Check for Discount on Adenovirus Diagnostic Testing Market: US, Europe, Japan report by Venture Planning Group. The report presents a detailed analysis of the Adenovirus diagnostics...
Premade Human MIR1538 Adenovirus (NR_031719), ready-to-use and available with your choice of HA tag, His tag, GFP reporter or untagged.
... , For the vaccination of healthy, susceptible horses 6 months of age or older, including pregnant mares, as an aid in the reduction of respiratory diseases
Translation regulator that binds to the 3-UTR of specific mRNAs such as nanos (nos) and prevents their translation. Prevents translation of unlocalized nos in the bulk cytoplasm via the recruitment of cup.
Adenovirus infection generally may lead to common cold, pneumonia and pharyngitis.There are few types of adenoviruses. Specifically, adenoviruses types 8,19 and 37 will lead to keratoconjunctivitis mostly in adult.
In one mode of adenovirus infection (called lytic infection because it destroys large numbers of cells), adenoviruses kill healthy cells and replicate up to 1 million new viruses per cell killed, of which 1 to 5 percent are infectious. People with this kind of infection feel sick. In chronic or latent infection, a much smaller number of viruses are released, and healthy cells can multiply more rapidly than they are destroyed. People who have this kind of infection do not exhibit symptoms. Children who have normal immune systems usually experience only minor symptoms when infected with adenovirus. The course of infection tends to be more serious in children who are immunocompromised, such as those undergoing chemotherapy or those who have a disease that disrupts normal immune response (e.g. human immunodeficiency syndrome [HIV]). In such children, the virus more often affects organs such as the lungs, liver, and kidneys, and the risk of fatality increases. Symptoms common to respiratory illnesses ...
Doctors Ask: Adenovirus infection is exposed to all segments of the population. Basic manifestations of adenovirus infection The latent (incubation period) is usually 5-7 days. The disease most often begins acutely, from the phenomena of intoxication: slight increase in temperature, rashes in the body, chills, lethargy, headache, loss of appetite, etc.
SignaGen Laboratories, A Gene Delivery Company Providing Custom AAV Adenovirus Lentivirus Production Services & Manufacturing DNA/siRNA Transfection Reagents... CAPN2 Adenovirus [SL102580] - Product InformationProduct Name: Calpain 2, (m/II) Large Subunit, cloned into our Ad-MAX adenovirus expression system, ready to package.Promoter: CMVStorage Buffer: DMEM with 2.5% BSA, 2.5% glycerolTurnaround Time: 5~6 weeksGene InformationGene Name: Calpain 2, (m/II) Large SubunitNCBI Acc. #: BC021303Gene Symbol: CAPN2Gene ID: 824GI Number: 18204176Fusion Tag: No Customized service
Adenoviruses are a group of medium sized, non enveloped, ds DNA viruses that share a common complement fixing antigen. They infect humans, animals and birds, shows strict host specifity. At least 47 serotypes of adenoviruses have been isolated from human sources. Adenovirus infections are common worldwide mostly in children. Many infections are asymptomatic. The virus may persist in the host for many months.It cause infections of the respiratory tract and eyes. These viruses carry DNA up to 7kb and are being investigated as potential vectors in gene therapy. ...
What is the difference between Adenovirus and Retrovirus? Adenovirus is a virus type without an envelope whereas retroviruses are virus type with an envelope...
U C L A A L Z H E I M E R S D I S E A S E C E N T E RA L Z H E I M E R S D I S E A S E R E S E A R C H C E N T E R O F C A L I F O R N I AK AT H E R I N E & B E N J A M I N K A G A N A L Z H E I M E R S D I S E A S E T R E AT M E N T P R O G R A M The UCLA Focal- type Dementias Clinic The UCLA Focal- type Dementias Clinic (FtD) specializes in the diagnosis and management ...
Adenoviruses are a family of viruses that are an important cause of febrile illnesses in young children. They are most frequently associated with upper respiratory tract syndromes, such as pharyngitis or coryza, but can also cause pneumonia. Less com
A new study explains how a genetically altered version of the adenovirus may improve the efficacy of mesothelioma chemotherapy drugs.
Premade Mouse CAPRIN1 (caprin-1 isoform a) Adenovirus (NM_001111289), ready-to-use and available with your choice of HA tag, His tag, GFP reporter or untagged.
Esta pesquisa tem por objetivo repertoriar os termos do licenciamento ambiental brasileiro e oferecer equivalentes em língua inglesa, visando à construção futura de um glossário deste tema. A motivação para a pesquisa advém da necessidade de criar um instrumento que possa contribuir para a comunicação entre profissionais do meio ambiente e o público estrangeiro, cujo benefício se estende aos profissionais da tradução, intermediadores da comunicação. A pesquisa se realiza em dois corpora, compostos de legislação ambiental e de manuais de licenciamento ambiental voltados a orientar aos cidadãos, nos idiomas português e inglês. Para a sua realização, recorreu-se aos pressupostos teórico-metodológicos da Teoria Comunicativa da Terminologia (CABRÉ, 1999a), que compreende os termos como unidades léxicas pertencentes às línguas naturais, que adquirem estatuto terminológico no âmbito das comunicações especializadas. Na Linguística de Corpus (SARDINHA, 2004), disciplina ...
眼窩静脈叢採取血液と心臓採取血液の血液生化学値の比較-眼窩静脈叢採取血液での酵素値の上昇- / p99 (0165.jp2 ...
Watch the video, get the download or listen to The Shitty Limits - Swallowed Whole for free. Swallowed Whole appears on the album Beware The Limits. Discover more music, gig and concert tickets, videos, lyrics, free downloads and MP3s, and photos with the largest catalogue online at Last.fm.
Safety and Immunogenicity of Recombinant DNA and Adenovirus Expressing L523S Protein in Early Stage Non-Small Cell Lung Cancer ...  Safety and Immunogenicity of Recombinant DNA and Adenovirus Expressing L523S Protein in Early Stage Non-Small Cell Lung Cancer ...
... of a therapeutic vaccine regimen with recombinant DNA and adenovirus expressing L523S protein in patients with early stage non- ... Immunogenicity Of Sequential Administration Of Recombinant DNA And Adenovirus Expressing L523S Protein In Patients With Early ... To provide initial evidence as to whether CD8+ and CD4+ T cell responses specific for L523S protein can be elicited by two ...
more infohttps://clinicaltrials.gov/ct2/show/NCT00062907?recr=Open&cond=%22Adenoviridae+Infections%22&rank=18
Adenovirus Early Proteins
     Summary Report | CureHunter  Adenovirus Early Proteins Summary Report | CureHunter
Proteins encoded by adenoviruses that are synthesized prior to, and in the absence of, viral DNA replication. The proteins are ... E29 Protein, Adenovirus; E29 Proteins, Adenovirus; Early Proteins, Adenovirus; Protein, Adenovirus E19; Protein, Adenovirus E29 ... Proteins, Adenovirus E19; Proteins, Adenovirus E29; Adenovirus E19 Proteins; Adenovirus E29 Proteins; Adenovirus Early Region ... Neoplasm Proteins: 2*Oncogene Proteins: 1748*Viral Oncogene Proteins: 10*Adenovirus Early Proteins: 2*Adenovirus E3 Proteins: 2 ...
more infohttp://www.curehunter.com/public/keywordSummaryD017456-Adenovirus-Early-Proteins.do
Cytotoxicity of a recombinant fusion protein of adenovirus early region 4 open reading frame 4 (E4orf4) and human epidermal...  Cytotoxicity of a recombinant fusion protein of adenovirus early region 4 open reading frame 4 (E4orf4) and human epidermal...
This study presents an approach for inhibiting p53-deficient tumor cell growth by using protein-based E4orf4 ... protein is a novel cell death factor that selectively induces p53-independent apoptosis in cancer cells, but not in normal ... Adenovirus early region 4 open reading frame 4 (E4orf4) protein is a novel cell death factor that selectively induces p53- ... Cytotoxicity of a recombinant fusion protein of adenovirus early region 4 open reading frame 4 (E4orf4) and human epidermal ...
more infohttps://insights.ovid.com/pubmed?pmid=16702809
Hearing P[au] - PubMed - NCBI  Hearing P[au] - PubMed - NCBI
Adenovirus core protein VII protects the viral genome from a DNA damage response at early times after infection. ... E2F/Rb Family Proteins Mediate Interferon Induced Repression of Adenovirus Immediate Early Transcription to Promote Persistent ... Cellular proteins PML and Daxx mediate an innate antiviral defense antagonized by the adenovirus E4 ORF3 protein. ... Characterization of Empty adenovirus particles assembled in the absence of a functional adenovirus IVa2 protein. ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed?cmd=search&term=Hearing+P%5Bau%5D&dispmax=50
Molecular Analyses of MHC Antigens | SpringerLink  Molecular Analyses of MHC Antigens | SpringerLink
S.R. Ross, S.J. Flint, and A.J. Levine, Identification of the adenovirus early proteins and their genomic map positions. ... P.A. Peterson, and L. Rask, HLA-DR transplantation antigen chain is a member of the same protein superfamily as the ... J. Hérissé, G. Courtois, and F. Galivert, Nucleotide sequence of the EcoRI D fragment of adenovirus 2-genome. Nucleic Acids Res ... Transplantation antigens are encoded by eight exons that correlate with protein domains. Cell 25: 683-692 (1981)PubMedCrossRef ...
more infohttps://link.springer.com/chapter/10.1007/978-1-4684-4469-8_3
Glossary of Medical Terms | Hepatitis Central  Glossary of Medical Terms | Hepatitis Central
Adenovirus Early Proteins. Proteins encoded by adenoviruses that are synthesized prior to, and in the absence of, viral DNA ... Adenovirus E1A Proteins and Adenovirus E1B Proteins). Adenovirus E1A Proteins. Proteins transcribed from the E1A region of ... Adenovirus E4 Proteins. Proteins transcribed from the E4 region of adenovirus. The E4 19K protein transactivates transcription ... adenovirus which are involved in positive regulation of transcription of the early genes. Adenovirus E1B Proteins. Proteins ...
more infohttp://www.hepatitiscentral.com/hcv/glossary/a/
Richard J. Grand, MD | Boston Childrens Hospital  Richard J. Grand, MD | Boston Children's Hospital
Adenovirus type 12 early region 1 proteins: a study of their subcellular localization and protein-protein interactions. J Gen ... C-terminal-binding protein interacting protein binds directly to adenovirus early region 1A through its N-terminal region and ... Control of p53 expression by adenovirus 12 early region 1A and early region 1B 54K proteins. Virology. 1996 Apr 01; 218(1):23- ... Consequences of disruption of the interaction between p53 and the larger adenovirus early region 1B protein in adenovirus E1 ...
more infohttp://www.childrenshospital.org/directory/physicians/g/richard-grand
Early Events during BK Virus Entry and Disassembly | Journal of Virology  Early Events during BK Virus Entry and Disassembly | Journal of Virology
Association of adenovirus early-region 1A proteins with cellular polypeptides. Mol. Cell. Biol. 6:1579-1589. ... The Derlin family proteins are ER transmembrane proteins that are important for targeting certain misfolded proteins from the ... The ER protein disulfide isomerase or other protein disulfide isomerase-like proteins can cause conformational changes in VP1 ... Adenovirus protein VI mediates membrane disruption following capsid disassembly. J. Virol. 79:1992-2000. ...
more infohttps://jvi.asm.org/content/83/3/1350?ijkey=714a0f9d8f984f744336fd192a5bd32723c4efc4&keytype2=tf_ipsecsha
NAVER Academic > Search...  NAVER Academic > Search...
Adenoviridae, genetics, Adenovirus Early Proteins, Amino Acid Sequence, Base Sequence, Binding Sites, physiology, ... Adenoviridae, genetics, Adenovirus Early Proteins, Amino Acid Sequence, Binding Sites, physiology, Blotting, Western, ... Transcriptional repression by YY1, a human GLI-Krüppel-related protein, and relief of repression by adenovirus E1A protein.. ... Adenovirus E1A activation domain binds the basic repeat in the TATA box transcription factor.. 1991 W S Lee et al. CELL cited ...
more infohttps://academic.naver.com/search.naver?field=3&query=CELL%2067%EA%B6%8C%202%ED%98%B8
NAVER Academic > Search...  NAVER Academic > Search...
Adenovirus Early Proteins, Adenoviruses, Human, immunology, physiology, Biological Transport, Cell Membrane, HLA Antigens, HeLa ... Base Sequence, Binding Sites, DNA, Fungal, metabolism, DNA-Binding Proteins, Deoxyribonuclease I, Fungal Proteins, Gene ... Interaction of a gene-specific transcription factor with the adenovirus major late promoter upstream of the TATA box region.. M ... Impaired intracellular transport of class I MHC antigens as a possible means for adenoviruses to evade immune surveillance.. M ...
more infohttps://academic.naver.com/search.naver?field=3&query=CELL+43%EA%B6%8C+1%ED%98%B8
The J Domain of Simian Virus 40 Large T Antigen Is Required To Functionally Inactivate RB Family Proteins | Molecular and...  The J Domain of Simian Virus 40 Large T Antigen Is Required To Functionally Inactivate RB Family Proteins | Molecular and...
1988) Two regions of the adenovirus early region 1A proteins are required for transformation. J. Virol. 62:257-265. ... This is reminiscent of the adenovirus E1A oncoprotein, which also targets the RB family of proteins. Like TAg, adenovirus E1A ... 1992) Adenovirus E1A makes two distinct contacts with the retinoblastoma protein. J. Virol. 66:4606-4611. ... 1993) Independent regions of adenovirus E1A are required for binding to and dissociation of E2F-protein complexes. Mol. Cell. ...
more infohttps://mcb.asm.org/content/18/3/1408?ijkey=ce30b10c58d844f3e702233298b911df050f998f&keytype2=tf_ipsecsha
Oncolytic adenovirus - Wikipedia  Oncolytic adenovirus - Wikipedia
Yew, P.; Berk, A. (1992). "Inhibition of p53 transactivation required for transformation by adenovirus early 1B protein". ... and the knob domain of the adenovirus coat protein trimer. CAR is necessary for adenovirus infection. Although expressed widely ... For adenovirus replication to occur, the host cell must be induced into S phase by viral proteins interfering with cell cycle ... E1B proteins produced by adenoviruses protect the infected cell by binding to and degrading the p53 transcription factors, ...
more infohttps://en.wikipedia.org/wiki/Oncolytic_adenovirus
List of MeSH codes (D12.776) - Wikipedia  List of MeSH codes (D12.776) - Wikipedia
... oncogene protein tpr-met MeSH D12.776.624.664.520.045 - adenovirus early proteins MeSH D12.776.624.664.520.045.050 - adenovirus ... adenovirus e2 proteins MeSH D12.776.624.664.520.045.070 - adenovirus e3 proteins MeSH D12.776.624.664.520.045.080 - adenovirus ... adenovirus e1a proteins MeSH D12.776.964.700.045.050.110 - adenovirus e1b proteins MeSH D12.776.964.700.045.060 - adenovirus e2 ... adenovirus e3 proteins MeSH D12.776.964.700.045.080 - adenovirus e4 proteins MeSH D12.776.964.700.750.320 - fusion proteins, ...
more infohttps://en.wikipedia.org/wiki/List_of_MeSH_codes_(D12.776)
Tsukamoto AS[au] - PubMed - NCBI  Tsukamoto AS[au] - PubMed - NCBI
An immunodominant domain in adenovirus type 2 early region 1A proteins.. Tsukamoto AS, Ferguson B, Rosenberg M, Weissman IL, ... Genetic mapping of a major site of phosphorylation in adenovirus type 2 E1A proteins. ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed?cmd=search&term=Tsukamoto+AS%5Bau%5D&dispmax=50
Indian Patents. 250691:METHODS TO OBTAIN RECOMBINANT PROTEINS WITH INCREASED SIALYLATION FROM CELLS THAT EXPRESS ADENOVIRUS E1A...  Indian Patents. 250691:METHODS TO OBTAIN RECOMBINANT PROTEINS WITH INCREASED SIALYLATION FROM CELLS THAT EXPRESS ADENOVIRUS E1A...
early promoter can be trans-activated by adenovirus early proteins. Virology 171, 377-385. Grabenhorst E, Hoffmann A, Nimtz M, ... Thus, two aspects appear relevant for increasing sialylation of produced proteins in cells that express adenovirus El A protein ... METHODS TO OBTAIN RECOMBINANT PROTEINS WITH INCREASED SIALYLATION FROM CELLS THAT EXPRESS ADENOVIRUS E1A PROTEIN. ... INVENTION Methods to obtain recombinant proteins with increased sialylation from cells that express adenovirus El A protein, ...
more infohttp://www.allindianpatents.com/patents/250691-methods-to-obtain-recombinant-proteins-with-increased-sialylation-from-cells-that-express-adenovirus-e1a-protein
Use of Macrophages to Target Therapeutic Adenovirus to Human Prostate Tumors | Cancer Research  Use of Macrophages to Target Therapeutic Adenovirus to Human Prostate Tumors | Cancer Research
The role of human adenovirus early region 3 proteins (gp19K, 10.4K, 14.5K, and 14.7K) in a murine pneumonia model. J Virol 1996 ... Macrophage delivery of adenovirus to hypoxic areas of human prostate tumors. *. Delivery of a GFP-expressing adenovirus to ... Plasmid construction and adenoviruses. The techniques for constructing HRE-regulated E1A/B plasmids and adenoviruses (CMV-AdV5- ... upregulated E1A protein, and released multiple copies of adenovirus. The virus then infected neighboring cells but only ...
more infohttp://cancerres.aacrjournals.org/content/71/5/1805
Team | Research | Helmholtz Centre for Infection Research  Team | Research | Helmholtz Centre for Infection Research
H. Esche, M. Reuther and K. Schughart (1984) Early and late proteins of adenovirus type 12: translation. mapping with RNA ... K. Schughart, E. Bause and H. Esche (1985) Structure and expression of adenovirus type 12 E1B 58K protein. in infected and ... K. Schughart, B. von Wilcken-Bergmann and H. Esche (1987) Expression of adenovirus type 12 E1b 58-kDa protein. in Escherichia ... Publications from earlier years. W. Kramer, K. Schughart and H.-J. Fritz (1982) Directed mutagenesis of DNA cloned in ...
more infohttps://www.helmholtz-hzi.de/en/nc/research/research-topics/bacterial-and-viral-pathogens/infection-genetics/team/personal/infos/klaus-schughart/
Immune evasion: Who needs it? | Mystery Rays from Outer Space  Immune evasion: Who needs it? | Mystery Rays from Outer Space
The role of human adenovirus early region 3 proteins (gp19K, 10.4K, 14.5K, and 14.7K) in a murine pneumonia model. Sparer TE, ... The AdF CR1 proteins have two domains, a V-set like Ig domain (which has similarity to CR1 proteins from other adenovirus ... protein thats long been shown to block MHC class I antigen processing. In particular, mouse adenovirus 1 does NOT block ... "the E3 protein gp19K alters neither afferent nor efferent immune responses"). However, human adenoviruses dont replicate well ...
more infohttp://www.iayork.com/MysteryRays/2007/08/06/33/
Pazopanib(GW-786034) manufacture | immune-source.com  Pazopanib(GW-786034) manufacture | immune-source.com
Acf1 overexpression prevents downregulation of an early adenovirus protein by E4orf4 during viral infection. Our results ... manufacture cellular proteins (4,5), facilitating alternate splicing of adenovirus mRNAs (5), and regulating protein ... The adenovirus Y4 open-reading-frame 4 (Y4orf4) protein regulates the progression of. immune Adenosine A2A Receptors LEG8 ... The adenovirus Y4 open-reading-frame 4 (Y4orf4) protein regulates the progression of viral infection and when expressed ...
more infohttp://www.immune-source.com/tag/pazopanibgw-786034-manufacture/
Adenosine A2A Receptors  Adenosine A2A Receptors
Acf1 overexpression prevents downregulation of an early adenovirus protein by E4orf4 during viral infection. Our results ... manufacture cellular proteins (4,5), facilitating alternate splicing of adenovirus mRNAs (5), and regulating protein ... The adenovirus Y4 open-reading-frame 4 (Y4orf4) protein regulates the progression of. immune Adenosine A2A Receptors LEG8 ... The adenovirus Y4 open-reading-frame 4 (Y4orf4) protein regulates the progression of viral infection and when expressed ...
more infohttp://www.immune-source.com/category/adenosine-a2a-receptors/
  • Based on cryo-electron microscopy analysis of BKV-like particles ( 26 ) and the crystal structures of mouse polyomavirus (MPyV) and SV40 ( 51 , 52 ), the BKV major capsid protein VP1 forms the outer shell of the capsid, with the minor capsid proteins VP2 and VP3 forming a bridge linking the VP1 shell and the viral genome. (asm.org)
  • Synergistic up-regulation of the myeloid-specific promoter for the macrophage colony-stimulating factor receptor by AML1 and the t(8;21) fusion protein may contribute to leukenogenesis. (atcc.org)
  • We therefore undertook an extensive site-directed mutagenesis approach to generate specific point mutants and to precisely map the binding sites for CBP, p300, TATA-binding protein (TBP), S4, S8, hGcn5, P/CAF, and Ran within the first 30 amino acids of the Ad5 12S E1A protein. (asm.org)
  • Interestingly, although TBP and S8 or CBP/p300 can exist as functional complexes, RNA interference revealed that the recruitment of either TBP, S8, or CBP/p300 to AdE1A was not dependent upon the expression of the other proteins. (asm.org)
  • Consistent with previous studies, CBP/p300 was found to be targeted by AdE1A during this process, although our data suggest that binding to other N-terminal proteins is also important for transformation. (asm.org)
  • The N-terminal region and elements in CR1 are required for binding the transcriptional coactivator proteins p300 and CBP ( 1 , 15 ). (asm.org)
  • However, it is still unclear in most cases whether these receptors interact with rafts through lipid-based amino acid modifications or whether raft insertion is driven by protein-protein interactions. (jimmunol.org)
  • Mechanism of Adenovirus E4-ORF3-Mediated SUMO Modifications. (nih.gov)
  • As far as I know, which isn't all that far, the mechanism by which adenoviruses are able to do this isn't well understood. (iayork.com)
  • These data further indicate that AdE1A can target individual partner proteins in vivo and that it does not necessarily recruit these proteins indirectly as components of larger macromolecular complexes. (asm.org)
  • Adapter molecules Bi-specific adapter molecules can be administered along with the virus to redirect viral coat protein tropism. (wikipedia.org)
  • These molecules are fusion proteins that are made up of an antibody raised against the knob domain of the adenovirus coat protein, fused to a natural ligand for a cell-surface receptor. (wikipedia.org)
  • By integrating reverse transcription, padlock probes, and rolling circle amplification, we were able to detect and differentiate between viral RNA (vRNA) and complementary RNA (cRNA) molecules, and to detect viral protein within the same cell. (diva-portal.org)
  • Purification and characterization of a new anticoagulant protein, PP27, from placenta. (scialert.net)
  • An immunodominant domain in adenovirus type 2 early region 1A proteins. (nih.gov)
  • We determined that although common residues within the N-terminal region can form partial binding sites for these proteins, point mutants were also generated that could discriminate between binding sites. (asm.org)
  • These protein possess a similar ATPase domain but contain additional unique domains and associate with different regulatory subunits (28). (immune-source.com)
  • An RGD motif inserted into the HI loop of the fiber knob protein, shifts specificity toward integrins, which are frequently over-expressed in oesophageal adenocarcinoma. (wikipedia.org)
  • Finally, we took advantage of the fine-mapping data to ascertain which proteins were targeted during the transformation process. (asm.org)
  • The N terminus of TAg shares sequence homology with the J domain of the DnaJ (heat shock protein 40 [Hsp family of molecular chaperones ( 20 ). (asm.org)
  • So something keeps the protein sequence similar among similar viruses - it's strongly selected - but between subgroups it can diverge quite widely. (iayork.com)
  • The fiber protein also contains an HI-loop structure, which can tolerate peptide insertions of up to 100 residues without any negative effects on adenovirus integrity. (wikipedia.org)
  • The precise binding sites for these proteins, however, remain to be resolved. (asm.org)
  • These data indicate that AdE1A can target each of these proteins individually through distinct binding sites. (asm.org)
  • and (iii) an N-terminal domain that is homologous to the J domain of DnaJ molecular chaperone proteins. (asm.org)
  • These results imply that while the LXCXE domain serves as a binding site for the RB-related proteins, the J domain plays an important role in inactivating their function. (asm.org)
  • these interactions inhibit RBX1 - CUL1 -dependent elongation reaction of ubiquitin chains and attenuate ubiquitination of SCF( FBXW7 ) target proteins (PubMed:19679664). (rcsb.org)
  • Transport of proteins into mitochondria: translocational intermediates spanning contact sites between outer and inner membranes. (naver.com)
  • The ensuing switch in chromatin redesigning determines existence versus death decisions and contributes to Elizabeth4orf4 functions during adenovirus illness. (immune-source.com)
  • A great deal of new information has accumulated about specificity in the activation of the STATs, about the functional domains of the proteins, and about the variety of their biologic functions both in development and in adults. (sciencemag.org)
  • In this study, we show for the first time that macrophages can be engineered to silently transport to, and then produce high titres of therapeutic adenovirus specifically in hypoxic areas of experimental prostate tumors. (aacrjournals.org)