Adenovirus e1a proteins. Medical search

Proteins transcribed from the E1A genome region of ADENOVIRUSES which are involved in positive regulation of transcription of the early genes of host infection.

Species of the genus MASTADENOVIRUS, causing a wide range of diseases in humans. Infections are mostly asymptomatic, but can be associated with diseases of the respiratory, ocular, and gastrointestinal systems. Serotypes (named with Arabic numbers) have been grouped into species designated Human adenovirus A-F.

Proteins transcribed from the E4 region of ADENOVIRUSES. The E4 19K protein transactivates transcription of the adenovirus E2F protein and complexes with it.

Proteins transcribed from the E1B region of ADENOVIRUSES which are involved in regulation of the levels of early and late viral gene expression.

Proteins encoded by adenoviruses that are synthesized prior to, and in the absence of, viral DNA replication. The proteins are involved in both positive and negative regulation of expression in viral and cellular genes, and also affect the stability of viral mRNA. Some are also involved in oncogenic transformation.

Proteins transcribed from the E3 region of ADENOVIRUSES but not essential for viral replication. The E3 19K protein mediates adenovirus persistence by reducing the expression of class I major histocompatibility complex antigens on the surface of infected cells.

A family of non-enveloped viruses infecting mammals (MASTADENOVIRUS) and birds (AVIADENOVIRUS) or both (ATADENOVIRUS). Infections may be asymptomatic or result in a variety of diseases.

Respiratory and conjunctival infections caused by 33 identified serotypes of human adenoviruses.

Proteins transcribed from the E2 region of ADENOVIRUSES. Several of these are required for viral DNA replication.

Virus diseases caused by the ADENOVIRIDAE.

The very first viral gene products synthesized after cells are infected with adenovirus. The E1 region of the genome has been divided into two major transcriptional units, E1A and E1B, each expressing proteins of the same name (ADENOVIRUS E1A PROTEINS and ADENOVIRUS E1B PROTEINS).

Products of viral oncogenes, most commonly retroviral oncogenes. They usually have transforming and often protein kinase activities.

DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.

The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.

Established cell cultures that have the potential to propagate indefinitely.

A genus of ADENOVIRIDAE that infects MAMMALS including humans and causes a wide range of diseases. The type species is Human adenovirus C (see ADENOVIRUSES, HUMAN).

Species of the genus MASTADENOVIRUS that causes fever, edema, vomiting, and diarrhea in dogs and encephalitis in foxes. Epizootics have also been caused in bears, wolves, coyotes, and skunks. The official species name is Canine adenovirus and it contains two serotypes.

Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.

The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.

The functional hereditary units of VIRUSES.

Deoxyribonucleic acid that makes up the genetic material of viruses.

An inheritable change in cells manifested by changes in cell division and growth and alterations in cell surface properties. It is induced by infection with a transforming virus.

A member of the p300-CBP transcription factors that was originally identified as a binding partner for ADENOVIRUS E1A PROTEINS.

DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.

Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.

The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.

Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.

The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.

Any of the processes by which cytoplasmic factors influence the differential control of gene action in viruses.

Product of the retinoblastoma tumor suppressor gene. It is a nuclear phosphoprotein hypothesized to normally act as an inhibitor of cell proliferation. Rb protein is absent in retinoblastoma cell lines. It also has been shown to form complexes with the adenovirus E1A protein, the SV40 T antigen, and the human papilloma virus E7 protein.

Those proteins recognized by antibodies from serum of animals bearing tumors induced by viruses; these proteins are presumably coded for by the nucleic acids of the same viruses that caused the neoplastic transformation.

The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.

A transcription factor that possesses DNA-binding and E2F-binding domains but lacks a transcriptional activation domain. It is a binding partner for E2F TRANSCRIPTION FACTORS and enhances the DNA binding and transactivation function of the DP-E2F complex.

Species of the genus MASTADENOVIRUS, causing neurological disease in pigs.

Proteins found in any species of virus.

A genus of ADENOVIRIDAE that infects birds. The type species is FOWL ADENOVIRUS A.

A ubiquitously expressed regulatory protein that contains a retinoblastoma protein binding domain and an AT-rich interactive domain. The protein may play a role in recruiting HISTONE DEACETYLASES to the site of RETINOBLASTOMA PROTEIN-containing transcriptional repressor complexes.

Techniques and strategies which include the use of coding sequences and other conventional or radical means to transform or modify cells for the purpose of treating or reversing disease conditions.

Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.

A family of basic helix-loop-helix transcription factors that control expression of a variety of GENES involved in CELL CYCLE regulation. E2F transcription factors typically form heterodimeric complexes with TRANSCRIPTION FACTOR DP1 or transcription factor DP2, and they have N-terminal DNA binding and dimerization domains. E2F transcription factors can act as mediators of transcriptional repression or transcriptional activation.

The type species of the genus AVIADENOVIRUS, family ADENOVIRIDAE, an oncogenic virus of birds. This is also called CELO virus for chick embryo lethal orphan virus.

The introduction of functional (usually cloned) GENES into cells. A variety of techniques and naturally occurring processes are used for the gene transfer such as cell hybridization, LIPOSOMES or microcell-mediated gene transfer, ELECTROPORATION, chromosome-mediated gene transfer, TRANSFECTION, and GENETIC TRANSDUCTION. Gene transfer may result in genetically transformed cells and individual organisms.

Processes that stimulate the GENETIC TRANSCRIPTION of a gene or set of genes.

The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.

Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.

Proteins that form the CAPSID of VIRUSES.

Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.

Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.

Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.

Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.

The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.

Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.

A species of POLYOMAVIRUS originally isolated from Rhesus monkey kidney tissue. It produces malignancy in human and newborn hamster kidney cell cultures.

The parts of a macromolecule that directly participate in its specific combination with another molecule.

Eukaryotic cell line obtained in a quiescent or stationary phase which undergoes conversion to a state of unregulated growth in culture, resembling an in vitro tumor. It occurs spontaneously or through interaction with viruses, oncogenes, radiation, or drugs/chemicals.

Specific molecular components of the cell capable of recognizing and interacting with a virus, and which, after binding it, are capable of generating some signal that initiates the chain of events leading to the biological response.

Nuclear phosphoprotein encoded by the p53 gene (GENES, P53) whose normal function is to control CELL PROLIFERATION and APOPTOSIS. A mutant or absent p53 protein has been found in LEUKEMIA; OSTEOSARCOMA; LUNG CANCER; and COLORECTAL CANCER.

Use of attenuated VIRUSES as ANTINEOPLASTIC AGENTS to selectively kill CANCER cells.

Activating transcription factors were originally identified as DNA-BINDING PROTEINS that interact with early promoters from ADENOVIRUSES. They are a family of basic leucine zipper transcription factors that bind to the consensus site TGACGTCA of the cyclic AMP response element, and are closely related to CYCLIC AMP-RESPONSIVE DNA-BINDING PROTEIN.

RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.

Polyomavirus antigens which cause infection and cellular transformation. The large T antigen is necessary for the initiation of viral DNA synthesis, repression of transcription of the early region and is responsible in conjunction with the middle T antigen for the transformation of primary cells. Small T antigen is necessary for the completion of the productive infection cycle.

Tumor-selective, replication competent VIRUSES that have antineoplastic effects. This is achieved by producing cytotoxicity-enhancing proteins and/or eliciting an antitumor immune response. They are genetically engineered so that they can replicate in CANCER cells but not in normal cells, and are used in ONCOLYTIC VIROTHERAPY.

Simultaneous inflammation of the cornea and conjunctiva.

The transfer of bacterial DNA by phages from an infected bacterium to another bacterium. This also refers to the transfer of genes into eukaryotic cells by viruses. This naturally occurring process is routinely employed as a GENE TRANSFER TECHNIQUE.

Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)

A negative regulator of the CELL CYCLE that undergoes PHOSPHORYLATION by CYCLIN-DEPENDENT KINASES. It contains a conserved pocket region that binds E2F4 TRANSCRIPTION FACTOR and interacts with viral ONCOPROTEINS such as POLYOMAVIRUS TUMOR ANTIGENS; ADENOVIRUS E1A PROTEINS; and PAPILLOMAVIRUS E7 PROTEINS.

A member of the p300-CBP transcription factor family that was initially identified as a binding partner for CAMP RESPONSE ELEMENT-BINDING PROTEIN. Mutations in CREB-binding protein are associated with RUBINSTEIN-TAYBI SYNDROME.

Cell changes manifested by escape from control mechanisms, increased growth potential, alterations in the cell surface, karyotypic abnormalities, morphological and biochemical deviations from the norm, and other attributes conferring the ability to invade, metastasize, and kill.

An enzyme that catalyzes the acetylation of chloramphenicol to yield chloramphenicol 3-acetate. Since chloramphenicol 3-acetate does not bind to bacterial ribosomes and is not an inhibitor of peptidyltransferase, the enzyme is responsible for the naturally occurring chloramphenicol resistance in bacteria. The enzyme, for which variants are known, is found in both gram-negative and gram-positive bacteria. EC 2.3.1.28.

Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.

One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.

The process by which a DNA molecule is duplicated.

ONCOGENE PROTEINS from papillomavirus that deregulate the CELL CYCLE of infected cells and lead to NEOPLASTIC CELL TRANSFORMATION. Papillomavirus E7 proteins have been shown to interact with various regulators of the cell cycle including RETINOBLASTOMA PROTEIN and certain cyclin-dependent kinase inhibitors.

The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.

An E2F transcription factor that interacts directly with RETINOBLASTOMA PROTEIN and CYCLIN A and activates GENETIC TRANSCRIPTION required for CELL CYCLE entry and DNA synthesis. E2F1 is involved in DNA REPAIR and APOPTOSIS.

Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release.

Ribonucleic acid that makes up the genetic material of viruses.

Genes whose gain-of-function alterations lead to NEOPLASTIC CELL TRANSFORMATION. They include, for example, genes for activators or stimulators of CELL PROLIFERATION such as growth factors, growth factor receptors, protein kinases, signal transducers, nuclear phosphoproteins, and transcription factors. A prefix of "v-" before oncogene symbols indicates oncogenes captured and transmitted by RETROVIRUSES; the prefix "c-" before the gene symbol of an oncogene indicates it is the cellular homolog (PROTO-ONCOGENES) of a v-oncogene.

This line KB is now known to be a subline of the ubiquitous KERATIN-forming tumor cell line HeLa. It was originally thought to be derived from an epidermal carcinoma of the mouth, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via contamination by HELA CELLS. The cells are positive for keratin by immunoperoxidase staining. KB cells have been reported to contain human papillomavirus18 (HPV-18) sequences.

Inflammation, often mild, of the conjunctiva caused by a variety of viral agents. Conjunctival involvement may be part of a systemic infection.

Proteins that control the CELL DIVISION CYCLE. This family of proteins includes a wide variety of classes, including CYCLIN-DEPENDENT KINASES, mitogen-activated kinases, CYCLINS, and PHOSPHOPROTEIN PHOSPHATASES as well as their putative substrates such as chromatin-associated proteins, CYTOSKELETAL PROTEINS, and TRANSCRIPTION FACTORS.

Cis-acting DNA sequences which can increase transcription of genes. Enhancers can usually function in either orientation and at various distances from a promoter.

Transport proteins that carry specific substances in the blood or across cell membranes.

Vaccines used to prevent infection by any virus from the family ADENOVIRIDAE.

A conserved A-T rich sequence which is contained in promoters for RNA polymerase II. The segment is seven base pairs long and the nucleotides most commonly found are TATAAAA.

A sequence of successive nucleotide triplets that are read as CODONS specifying AMINO ACIDS and begin with an INITIATOR CODON and end with a stop codon (CODON, TERMINATOR).

A genus of the family PARVOVIRIDAE, subfamily PARVOVIRINAE, which are dependent on a coinfection with helper adenoviruses or herpesviruses for their efficient replication. The type species is Adeno-associated virus 2.

The outer protein protective shell of a virus, which protects the viral nucleic acid.

The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.

Proteins prepared by recombinant DNA technology.

Serologic tests in which a positive reaction manifested by visible CHEMICAL PRECIPITATION occurs when a soluble ANTIGEN reacts with its precipitins, i.e., ANTIBODIES that can form a precipitate.

The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.

A phosphoprotein phosphatase subtype that is comprised of a catalytic subunit and two different regulatory subunits. At least two genes encode isoforms of the protein phosphatase catalytic subunit, while several isoforms of regulatory subunits exist due to the presence of multiple genes and the alternative splicing of their mRNAs. Protein phosphatase 2 acts on a broad variety of cellular proteins and may play a role as a regulator of intracellular signaling processes.

Enzymes catalyzing the transfer of an acetyl group, usually from acetyl coenzyme A, to another compound. EC 2.3.1.

Genes that are introduced into an organism using GENE TRANSFER TECHNIQUES.

A general transcription factor that plays a major role in the activation of eukaryotic genes transcribed by RNA POLYMERASES. It binds specifically to the TATA BOX promoter element, which lies close to the position of transcription initiation in RNA transcribed by RNA POLYMERASE II. Although considered a principal component of TRANSCRIPTION FACTOR TFIID it also takes part in general transcription factor complexes involved in RNA POLYMERASE I and RNA POLYMERASE III transcription.

Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected.

The major sequence-specific DNA-binding component involved in the activation of transcription of RNA POLYMERASE II. It was originally described as a complex of TATA-BOX BINDING PROTEIN and TATA-BINDING PROTEIN ASSOCIATED FACTORS. It is now know that TATA BOX BINDING PROTEIN-LIKE PROTEINS may take the place of TATA-box binding protein in the complex.

Visible morphologic changes in cells infected with viruses. It includes shutdown of cellular RNA and protein synthesis, cell fusion, release of lysosomal enzymes, changes in cell membrane permeability, diffuse changes in intracellular structures, presence of viral inclusion bodies, and chromosomal aberrations. It excludes malignant transformation, which is CELL TRANSFORMATION, VIRAL. Viral cytopathogenic effects provide a valuable method for identifying and classifying the infecting viruses.

Products of proto-oncogenes. Normally they do not have oncogenic or transforming properties, but are involved in the regulation or differentiation of cell growth. They often have protein kinase activity.

A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.

Viruses which enable defective viruses to replicate or to form a protein coat by complementing the missing gene function of the defective (satellite) virus. Helper and satellite may be of the same or different genus.

A cell line derived from cultured tumor cells.

Tumor suppressor genes located on human chromosome 13 in the region 13q14 and coding for a family of phosphoproteins with molecular weights ranging from 104 kDa to 115 kDa. One copy of the wild-type Rb gene is necessary for normal retinal development. Loss or inactivation of both alleles at this locus results in retinoblastoma.

Head to tail array of covalently joined DNA sequences generated by concatenation. Concatenated DNA is attached end to end in contrast to CATENATED DNA which is attached loop to loop.

Viruses which lack a complete genome so that they cannot completely replicate or cannot form a protein coat. Some are host-dependent defectives, meaning they can replicate only in cell systems which provide the particular genetic function which they lack. Others, called SATELLITE VIRUSES, are able to replicate only when their genetic defect is complemented by a helper virus.

In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.

A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.

Mutant mice homozygous for the recessive gene "nude" which fail to develop a thymus. They are useful in tumor studies and studies on immune responses.

DNA viruses producing malignant tumors. Of the six major groupings of DNA viruses four contain members which are actually or potentially oncogenic: the Adenoviridae, the Herpesviridae, the Papovaviridae, and the Poxviridae.

A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).

Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.

A sequence of amino acids in a polypeptide or of nucleotides in DNA or RNA that is similar across multiple species. A known set of conserved sequences is represented by a CONSENSUS SEQUENCE. AMINO ACID MOTIFS are often composed of conserved sequences.

The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.

The sum of the weight of all the atoms in a molecule.

A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.

A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.

Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.

Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.

Genes whose expression is easily detectable and therefore used to study promoter activity at many positions in a target genome. In recombinant DNA technology, these genes may be attached to a promoter region of interest.

Biochemical identification of mutational changes in a nucleotide sequence.

Actual loss of portion of a chromosome.

Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). Lines have been developed using several different strains of mice. Tissues are usually fibroblasts derived from mouse embryos but other types and sources have been developed as well. The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION.

A negative regulator of the CELL CYCLE that undergoes PHOSPHORYLATION by CYCLIN-DEPENDENT KINASES. RBL2 contains a conserved pocket region that binds E2F4 TRANSCRIPTION FACTOR and E2F5 TRANSCRIPTION FACTOR. RBL2 also interacts with viral ONCOPROTEINS such as POLYOMAVIRUS TUMOR ANTIGENS; ADENOVIRUS E1A PROTEINS; and PAPILLOMAVIRUS E7 PROTEINS.

Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.

A genus of the family PICORNAVIRIDAE whose members preferentially inhabit the intestinal tract of a variety of hosts. The genus contains many species. Newly described members of human enteroviruses are assigned continuous numbers with the species designated "human enterovirus".

Structures that are part of or contained in the CELL NUCLEUS.

A family of histone acetyltransferases that is structurally-related to CREB-BINDING PROTEIN and to E1A-ASSOCIATED P300 PROTEIN. They function as transcriptional coactivators by bridging between DNA-binding TRANSCRIPTION FACTORS and the basal transcription machinery. They also modify transcription factors and CHROMATIN through ACETYLATION.

A subclass of enzymes which includes all dehydrogenases acting on primary and secondary alcohols as well as hemiacetals. They are further classified according to the acceptor which can be NAD+ or NADP+ (subclass 1.1.1), cytochrome (1.1.2), oxygen (1.1.3), quinone (1.1.5), or another acceptor (1.1.99).

An E2F transcription factor that interacts directly with RETINOBLASTOMA PROTEIN and CYCLIN A. E2F2 activates GENETIC TRANSCRIPTION required for CELL CYCLE entry and DNA synthesis.

A large family of regulatory proteins that function as accessory subunits to a variety of CYCLIN-DEPENDENT KINASES. They generally function as ENZYME ACTIVATORS that drive the CELL CYCLE through transitions between phases. A subset of cyclins may also function as transcriptional regulators.

Sequences of DNA or RNA that occur in multiple copies. There are several types: INTERSPERSED REPETITIVE SEQUENCES are copies of transposable elements (DNA TRANSPOSABLE ELEMENTS or RETROELEMENTS) dispersed throughout the genome. TERMINAL REPEAT SEQUENCES flank both ends of another sequence, for example, the long terminal repeats (LTRs) on RETROVIRUSES. Variations may be direct repeats, those occurring in the same direction, or inverted repeats, those opposite to each other in direction. TANDEM REPEAT SEQUENCES are copies which lie adjacent to each other, direct or inverted (INVERTED REPEAT SEQUENCES).

The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.

The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.

Cellular DNA-binding proteins encoded by the c-jun genes (GENES, JUN). They are involved in growth-related transcriptional control. There appear to be three distinct functions: dimerization (with c-fos), DNA-binding, and transcriptional activation. Oncogenic transformation can take place by constitutive expression of c-jun.

The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.

A genus of ADENOVIRIDAE that comprises viruses of several species of MAMMALS and BIRDS. The type species is Ovine adenovirus D.

Synthetic or natural oligonucleotides used in hybridization studies in order to identify and study specific nucleic acid fragments, e.g., DNA segments near or within a specific gene locus or gene. The probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the probe include the radioisotope labels 32P and 125I and the chemical label biotin.

Enzymes that oxidize certain LUMINESCENT AGENTS to emit light (PHYSICAL LUMINESCENCE). The luciferases from different organisms have evolved differently so have different structures and substrates.

A ubiquitously expressed zinc finger-containing protein that acts both as a repressor and activator of transcription. It interacts with key regulatory proteins such as TATA-BINDING PROTEIN; TFIIB; and ADENOVIRUS E1A PROTEINS.

Process of growing viruses in live animals, plants, or cultured cells.

A family of small, non-enveloped DNA viruses infecting birds and most mammals, especially humans. They are grouped into multiple genera, but the viruses are highly host-species specific and tissue-restricted. They are commonly divided into hundreds of papillomavirus "types", each with specific gene function and gene control regions, despite sequence homology. Human papillomaviruses are found in the genera ALPHAPAPILLOMAVIRUS; BETAPAPILLOMAVIRUS; GAMMAPAPILLOMAVIRUS; and MUPAPILLOMAVIRUS.

Tumor suppressor genes located on the short arm of human chromosome 17 and coding for the phosphoprotein p53.

A DNA-dependent RNA polymerase present in bacterial, plant, and animal cells. It functions in the nucleoplasmic structure where it transcribes DNA into RNA. It has specific requirements for cations and salt and has shown an intermediate sensitivity to alpha-amanitin in comparison to RNA polymerase I and II. EC 2.7.7.6.

Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.

Proteins that are normally involved in holding cellular growth in check. Deficiencies or abnormalities in these proteins may lead to unregulated cell growth and tumor development.

Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.

The complete genetic complement contained in a DNA or RNA molecule in a virus.

A species of CERCOPITHECUS containing three subspecies: C. tantalus, C. pygerythrus, and C. sabeus. They are found in the forests and savannah of Africa. The African green monkey (C. pygerythrus) is the natural host of SIMIAN IMMUNODEFICIENCY VIRUS and is used in AIDS research.

A genus of potentially oncogenic viruses of the family POLYOMAVIRIDAE. These viruses are normally present in their natural hosts as latent infections. The virus is oncogenic in hosts different from the species of origin.

Cellular DNA-binding proteins encoded by the c-myc genes. They are normally involved in nucleic acid metabolism and in mediating the cellular response to growth factors. Elevated and deregulated (constitutive) expression of c-myc proteins can cause tumorigenesis.

The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990)

Enzymes that catalyze acyl group transfer from ACETYL-CoA to HISTONES forming CoA and acetyl-histones.

Nuclear antigen with a role in DNA synthesis, DNA repair, and cell cycle progression. PCNA is required for the coordinated synthesis of both leading and lagging strands at the replication fork during DNA replication. PCNA expression correlates with the proliferation activity of several malignant and non-malignant cell types.

The rate dynamics in chemical or physical systems.

The ultimate exclusion of nonsense sequences or intervening sequences (introns) before the final RNA transcript is sent to the cytoplasm.

A diverse class of enzymes that interact with UBIQUITIN-CONJUGATING ENZYMES and ubiquitination-specific protein substrates. Each member of this enzyme group has its own distinct specificity for a substrate and ubiquitin-conjugating enzyme. Ubiquitin-protein ligases exist as both monomeric proteins multiprotein complexes.

The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.

A family of transcription factors found primarily in PLANTS that bind to the G-box DNA sequence CACGTG or to a consensus sequence CANNTG.

Invasion of the host RESPIRATORY SYSTEM by microorganisms, usually leading to pathological processes or diseases.

Production of new arrangements of DNA by various mechanisms such as assortment and segregation, CROSSING OVER; GENE CONVERSION; GENETIC TRANSFORMATION; GENETIC CONJUGATION; GENETIC TRANSDUCTION; or mixed infection of viruses.

A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.

Immunoglobulins produced in response to VIRAL ANTIGENS.

Proteins coded by oncogenes. They include proteins resulting from the fusion of an oncogene and another gene (ONCOGENE PROTEINS, FUSION).

A protein that has been shown to function as a calcium-regulated transcription factor as well as a substrate for depolarization-activated CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASES. This protein functions to integrate both calcium and cAMP signals.

Minute infectious agents whose genomes are composed of DNA or RNA, but not both. They are characterized by a lack of independent metabolism and the inability to replicate outside living host cells.

Process of determining and distinguishing species of bacteria or viruses based on antigens they share.

An area showing altered staining behavior in the nucleus or cytoplasm of a virus-infected cell. Some inclusion bodies represent "virus factories" in which viral nucleic acid or protein is being synthesized; others are merely artifacts of fixation and staining. One example, Negri bodies, are found in the cytoplasm or processes of nerve cells in animals that have died from rabies.

The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.

Process of generating a genetic MUTATION. It may occur spontaneously or be induced by MUTAGENS.

Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.

Inflammation of the lung parenchyma that is caused by a viral infection.

A suborder of PRIMATES consisting of six families: CEBIDAE (some New World monkeys), ATELIDAE (some New World monkeys), CERCOPITHECIDAE (Old World monkeys), HYLOBATIDAE (gibbons and siamangs), CALLITRICHINAE (marmosets and tamarins), and HOMINIDAE (humans and great apes).

Elements of limited time intervals, contributing to particular results or situations.

Substances elaborated by viruses that have antigenic activity.

Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.

A DNA-dependent RNA polymerase present in bacterial, plant, and animal cells. It functions in the nucleoplasmic structure and transcribes DNA into RNA. It has different requirements for cations and salt than RNA polymerase I and is strongly inhibited by alpha-amanitin. EC 2.7.7.6.

Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)

Membrane proteins encoded by the BCL-2 GENES and serving as potent inhibitors of cell death by APOPTOSIS. The proteins are found on mitochondrial, microsomal, and NUCLEAR MEMBRANE sites within many cell types. Overexpression of bcl-2 proteins, due to a translocation of the gene, is associated with follicular lymphoma.

A ubiquitously expressed complement receptor that binds COMPLEMENT C3B and COMPLEMENT C4B and serves as a cofactor for their inactivation. CD46 also interacts with a wide variety of pathogens and mediates immune response.

Deletion of sequences of nucleic acids from the genetic material of an individual.

Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.

A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.

The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.

Suspensions of attenuated or killed viruses administered for the prevention or treatment of infectious viral disease.

The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.

Motifs in DNA- and RNA-binding proteins whose amino acids are folded into a single structural unit around a zinc atom. In the classic zinc finger, one zinc atom is bound to two cysteines and two histidines. In between the cysteines and histidines are 12 residues which form a DNA binding fingertip. By variations in the composition of the sequences in the fingertip and the number and spacing of tandem repeats of the motif, zinc fingers can form a large number of different sequence specific binding sites.

Nucleic acid sequences involved in regulating the expression of genes.

CELL LINES derived from the CV-1 cell line by transformation with a replication origin defective mutant of SV40 VIRUS, which codes for wild type large T antigen (ANTIGENS, POLYOMAVIRUS TRANSFORMING). They are used for transfection and cloning. (The CV-1 cell line was derived from the kidney of an adult male African green monkey (CERCOPITHECUS AETHIOPS).)

INFLAMMATION of any segment of the GASTROINTESTINAL TRACT from ESOPHAGUS to RECTUM. Causes of gastroenteritis are many including genetic, infection, HYPERSENSITIVITY, drug effects, and CANCER.

The interaction of two or more substrates or ligands with the same binding site. The displacement of one by the other is used in quantitative and selective affinity measurements.

The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.

Viruses that produce tumors.

Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.

The directional growth of an organism in response to an external stimulus such as light, touch, or gravity. Growth towards the stimulus is a positive tropism; growth away from the stimulus is a negative tropism. (From Concise Dictionary of Biology, 1990)

A genus of the family HERPESVIRIDAE, subfamily BETAHERPESVIRINAE, infecting the salivary glands, liver, spleen, lungs, eyes, and other organs, in which they produce characteristically enlarged cells with intranuclear inclusions. Infection with Cytomegalovirus is also seen as an opportunistic infection in AIDS.

Injections introduced directly into localized lesions.

B-MYB transactivates its own promoter through SP1-binding sites. (1/910)

B-MYB is an ubiquitous protein required for mammalian cell growth. In this report we show that B-MYB transactivates its own promoter through a 120 bp segment proximal to the transcription start site. The B-MYB-responsive element does not contain myb-binding sites and gel-shift analysis shows that SP1, but not B-MYB, protein contained in SAOS2 cell extracts binds to the 120 bp B-myb promoter fragment. B-MYB-dependent transactivation is cooperatively increased in the presence of SP1, but not SP3 overexpression. When the SP1 elements of the B-myb promoter are transferred in front of a heterologous promoter, an increased response to B-MYB results. In contrast, c-MYB, the prototype member of the Myb family, is not able to activate the luciferase construct containing the SP1 elements. With the use of an SP1-GAL4 fusion protein, we have determined that the cooperative activation occurs through the domain A of SP1. These observations suggest that B-MYB functions as a coactivator of SP1, and that diverse combinations of myb and SP1 sites may dictate the responsiveness of myb-target genes to the various members of the myb family. (+info)

A viral mechanism for inhibition of p300 and PCAF acetyltransferase activity. (2/910)

Nucleosomal histone modification is believed to be a critical step in the activation of RNA polymerase II-dependent transcription. p300/CBP and PCAF histone acetyltransferases (HATs) are coactivators for several transcription factors, including nuclear hormone receptors, p53, and Stat1alpha, and participate in transcription by forming an activation complex and by promoting histone acetylation. The adenoviral E1A oncoprotein represses transcriptional signaling by binding to p300/CBP and displacing PCAF and p/CIP proteins from the complex. Here, we show that E1A directly represses the HAT activity of both p300/CBP and PCAF in vitro and p300-dependent transcription in vivo. Additionally, E1A inhibits nucleosomal histone modifications by the PCAF complex and blocks p53 acetylation. These results demonstrate the modulation of HAT activity as a novel mechanism of transcriptional regulation. (+info)

Regulation of histone acetyltransferases p300 and PCAF by the bHLH protein twist and adenoviral oncoprotein E1A. (3/910)

Histone acetyltransferases (HAT) play a critical role in transcriptional control by relieving repressive effects of chromatin, and yet how HATs themselves are regulated remains largely unknown. Here, it is shown that Twist directly binds two independent HAT domains of acetyltransferases, p300 and p300/CBP-associated factor (PCAF), and directly regulates their HAT activities. The N terminus of Twist is a primary domain interacting with both acetyltransferases, and the same domain is required for inhibition of p300-dependent transcription by Twist. Adenovirus E1A protein mimics the effects of Twist by inhibiting the HAT activities of p300 and PCAF. These findings establish a cogent argument for considering the HAT domains as a direct target for acetyltransferase regulation by both a cellular transcription factor and a viral oncoprotein. (+info)

Recruitment of the retinoblastoma protein to c-Jun enhances transcription activity mediated through the AP-1 binding site. (4/910)

The retinoblastoma susceptibility gene product (RB) is a transcriptional modulator. One of the targets for this modulator effect is the AP-1 binding site within the c-jun and collagenase promoters. The physical interactions between RB and c-Jun were demonstrated by co-immunoprecipitation of these two proteins using anti-c-Jun or anti-RB antisera, glutathione S-transferase affinity matrix binding assays in vitro, and electrophoretic mobility shift assays. The C-terminal site of the leucine zipper of c-Jun mediated the interaction with RB. Although the B-pocket domain of RB alone bound to c-Jun, a second c-Jun binding site in the RB was also suggested. Mammalian two-hybrid-based assay provided corroborative evidence that transactivation of gene expression by RB required the C-terminal region of c-Jun. We conclude that RB enhances transcription activity mediated through the AP-1 binding site. Adenovirus E1A or human papillomavirus E7 inhibits RB-mediated transcription activity. These data reveal that the interactions between these two distinct classes of oncoproteins RB and c-Jun may be involved in controlling cell growth and differentiation mediated by transcriptional regulation. (+info)

Mutant p53 can provoke apoptosis in p53-deficient Hep3B cells with delayed kinetics relative to wild-type p53. (5/910)

Wild-type (wt) p53 frequently induces apoptosis when expressed in tumor cells whereas mutant p53 acts as an oncoprotein and consequently, stimulates cell proliferation. We report here exceptions to that rule. p53 conformational mutant 175H and DNA contact mutant 273H provoke apoptosis in human p53-deficient Hep3B hepatoma cells with delayed kinetics relative to wt p53. Similarly, c-Myc strongly stimulates apoptosis in these cells. In contrast, viral oncoproteins E1A and E7, and the cellular oncoprotein MDM-2, fail to elicit cytocidal responses. Efficient apoptotic cell death by mutant p53 requires oligomerization as 175H and 273H with deletions between amino acid residues 326 and 347 of the oligomerization domain are nontoxic. Apoptosis by mutant or wt p53 was significantly inhibited by the serine protease inhibitor AEBSF but not by the inactive analog AEBSA. Together, these results suggest that a wt p53-independent control mechanism is operational in Hep3B cells that eliminates cells upon sensing illegitimate proliferation signals originating from certain oncoproteins, including mutant p53 and Myc. We suggest that some tumor cell types lack p53 altogether because they tolerate neither wild-type nor mutant forms of the protein. (+info)

Differences in the interactions of oncogenic adenovirus 12 early region 1A and nononcogenic adenovirus 2 early region 1A with the cellular coactivators p300 and CBP. (6/910)

Association with the cellular coactivators p300 and CBP is required for the growth-regulatory function of adenoviral (Ad) early region 1A (E1A) proteins. E1A regions necessary for these interactions overlap with domains involved in the induction of tumours in immunocompetent rodents through highly oncogenic Ad12. Differences in the association of cellular factors with the respective E1A domains of Ad12 and nononcogenic Ad2 might therefore be involved in serotype-specific oncogenicity. We analyzed the interaction of the Ad12 E1A 235R protein with p300 and CBP. Here we demonstrate that in the case of Ad12, but not Ad2/5, amino acids (aa) 1-29 of E1A proteins are sufficient to bind the p300-C/H3 domain in vivo and wild-type p300 in vitro. The conserved arginine-2, which is essential for the interaction between Ad2 E1A and p300, was dispensable for the Ad12 E1A 235R-p300 interaction in vitro. In addition to the p300-C/H3 region, we identified a second domain within p300 (aa 1999-2200) binding to the 235R protein. Contrary to p300, the amino-terminus and CR1 are necessary to associate with CBP. The aa 1-29 of the 235R protein but not CR1 are essential for the repression of colTRE-driven gene expression. This repression function is strictly dependent on p300 but not on CBP. (+info)

Transcriptional regulation of the mouse ferritin H gene. Involvement of p300/CBP adaptor proteins in FER-1 enhancer activity. (7/910)

We previously identified a major enhancer of the mouse ferritin H gene (FER-1) that is central to repression of the ferritin H gene by the adenovirus E1A oncogene (Tsuji, Y., Akebi, N., Lam, T. K., Nakabeppu, Y., Torti, S. V., and Torti, F. M. (1995) Mol. Cell. Biol. 15, 5152-5164). To dissect the molecular mechanism of transcriptional regulation of ferritin H, E1A mutants were tested for their ability to repress FER-1 enhancer activity using cotransfection with ferritin H-chloramphenicol acetyltransferase (CAT) reporter constructs. Here we report that p300/CBP transcriptional adaptor proteins are involved in the regulation of ferritin H transcription through the FER-1 enhancer element. Thus, E1A mutants that failed to bind p300/CBP lost the ability to repress FER-1, whereas mutants of E1A that abrogated its interaction with Rb, p107, or p130 were fully functional in transcriptional repression. Transfection with E1A did not affect endogenous p300/CBP levels, suggesting that repression of FER-1 by E1A is not due to repression of p300/CBP synthesis, but to E1A and p300/CBP interaction. In addition, we have demonstrated that transfection of a p300 expression plasmid significantly activated ferritin H-CAT containing the FER-1 enhancer, but had a marginal effect on ferritin H-CAT with FER-1 deleted. Furthermore, both wild-type p300 and a p300 mutant that failed to bind E1A but retained an adaptor function restored FER-1 enhancer activity repressed by E1A. Sodium butyrate, an inhibitor of histone deacetylase, mimicked p300/CBP function in activation of ferritin H-CAT and elevation of endogenous ferritin H mRNA, suggesting that the histone acetyltransferase activity of p300/CBP or its associated proteins may contribute to the activation of ferritin H transcription. Recruitment of these broadly active transcriptional adaptor proteins for ferritin H synthesis may represent an important mechanism by which changes in iron metabolism are coordinated with other cellular responses mediated by p300/CBP. (+info)

The adenoviral E1A oncoproteins interfere with the growth-inhibiting effect of the cdk-inhibitor p21(CIP1/WAF1). (8/910)

The cdk-inhibitor p21(CIP1/WAF1) inhibits the activities of cyclin-dependent kinases and proliferating cell nuclear antigen, thereby repressing cell-cycle progression and DNA replication. Transforming oncogenes, such as E1A of human adenovirus 5 (Ad5), may interfere with such growth-inhibitory proteins. In this study, we show that in various Ad5E1-transformed cells, p21(CIP1/WAF1) is expressed and that, in general, expression is not downregulated. In addition, colony-formation assays show that in Ad5E1-transformed cells highly overexpressed p21(CIP1/WAF1) can still cause growth inhibition. FACS experiments indicate, however, that a G1 arrest induced by moderate overexpression of p21(CIP1/WAF1) can be overcome by E1A. The E1A proteins may interfere with the function of p21(CIP1/WAF1) by binding. Indeed, p21(CIP1/WAF1) binds with its cyclin/cdk-binding N terminus to the transforming N-terminal and CR1 region of the E1A proteins. Together, these results lend support to the model that E1A can interfere directly with p21(CIP1/WAF1) function and thereby stimulates cell growth. (+info)

Lillie, J. W.; Loewenstein, P. M.; Green, M. R.; Green, M. (1987). "Functional domains of adenovirus type 5 E1a proteins". Cell ... He discovered two of the proteins required for adenoviruses to transform cells, and also other proteins that usurp the infected ... In recent years, Green has focused his studies on the multifunctional adenovirus oncoprotein E1A, which is necessary for cell ... Loewenstein, P. M., Wu, S., Chiang, C., & Green, M. (2012). The adenovirus E1A N-terminal repression domain represses ...

https://en.wikipedia.org/wiki/Maurice_Green_(virologist)

Whyte, P (1988). "Association between an oncogene and an anti-oncogene: the adenovirus E1A proteins bind to the retinoblastoma ... "Association between an oncogene and an anti-oncogene: the adenovirus E1A proteins bind to the retinoblastoma gene product." ( ... Whyte, P (1989). "Cellular targets for transformation by the adenovirus E1A proteins". Cell. 56: 67-75. doi:10.1016/0092-8674( ... "Cellular targets for transformation by the adenovirus E1A proteins." cell 56.1 (1989): 67-75. National Academy of Sciences, ...

https://en.wikipedia.org/wiki/Ed_Harlow

"Mammalian Srb/Mediator complex is targeted by adenovirus E1A protein". Nature. 399 (6733): 276-9. Bibcode:1999Natur.399..276B. ... Nakayama M, Kikuno R, Ohara O (Nov 2002). "Protein-protein interactions between large proteins: two-hybrid screening using a ... This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which ... Ko L, Cardona GR, Chin WW (May 2000). "Thyroid hormone receptor-binding protein, an LXXLL motif-containing protein, functions ...

https://en.wikipedia.org/wiki/CRSP3

The protein encoded by this gene was first identified by its ability to bind the adenovirus E1A protein. The protein localizes ... a novel adenovirus E1A-associated protein that inhibits E1A transactivation". The EMBO Journal. 14 (13): 3159-69. doi:10.1002/j ... Masselink H, Bernards R (Mar 2000). "The adenovirus E1A binding protein BS69 is a corepressor of transcription through ... "Ubiquitin-dependent degradation of adenovirus E1A protein is inhibited by BS69". Biochemical and Biophysical Research ...

https://en.wikipedia.org/wiki/ZMYND11

Li Y, Graham C, Lacy S, Duncan AM, Whyte P (1993). "The adenovirus E1A-associated 130-kD protein is encoded by a member of the ... "Interaction between human cyclin A and adenovirus E1A-associated p107 protein". Science. 255 (5040): 87-90. doi:10.1126/science ... Cyclin-A2 is a protein that in humans is encoded by the CCNA2 gene. It is one of the two types of cyclin A: cyclin A1 is ... Cyclin A2 (Ccna2) is a key protein involved in the direction of mammalian cardiac myocytes to grow and divide, and has been ...

https://en.wikipedia.org/wiki/Cyclin_A2

"Adenovirus E1A proteins direct subcellular redistribution of Nek9, a NimA-related kinase". Journal of Cellular Physiology. 212 ... "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology. 3: 89. doi:10.1038/ ... "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology. 3 (1): 89. doi: ... Serine/threonine-protein kinase Nek9 is an enzyme that in humans is encoded by the NEK9 gene. NEK9 has been shown to interact ...

https://en.wikipedia.org/wiki/NEK9

This protein can influence splice site selection of adenovirus E1A pre-mRNA. It interacts with the oncoprotein TLS, and ... FUS-interacting serine-arginine-rich protein 1 is a protein that in humans is encoded by the SFRS13A gene. This gene product is ... "TLS-ERG leukemia fusion protein inhibits RNA splicing mediated by serine-arginine proteins". Mol. Cell. Biol. 20 (10): 3345-54 ... "TLS-ERG leukemia fusion protein inhibits RNA splicing mediated by serine-arginine proteins". Mol. Cell. Biol. 20 (10): 3345-54 ...

https://en.wikipedia.org/wiki/FUSIP1

Histone acetyltransferase p300 also known as p300 HAT or E1A-associated protein p300 (where E1A = adenovirus early region 1A) ... "Relief of YY1 transcriptional repression by adenovirus E1A is mediated by E1A-associated protein p300". Genes Dev. 9 (10): 1188 ... "Molecular cloning and functional analysis of the adenovirus E1A-associated 300-kD protein (p300) reveals a protein with ... This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. EP300 is closely related to ...

https://en.wikipedia.org/wiki/EP300

"Relief of YY1 transcriptional repression by adenovirus E1A is mediated by E1A-associated protein p300". Genes & Development. 9 ... "Relief of YY1 transcriptional repression by adenovirus E1A is mediated by E1A-associated protein p300". Genes & Development. 9 ... and relief of repression by adenovirus E1A protein". Cell. 67 (2): 377-88. doi:10.1016/0092-8674(91)90189-6. PMID 1655281. ... YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein ...

https://en.wikipedia.org/wiki/YY1

"Upregulation of the Golgi protein GP73 by adenovirus infection requires the E1A CtBP interaction domain". Virology. 301 (2): ... Golgi membrane protein 1 (GOLM1) also known as Golgi phosphoprotein 2 or Golgi membrane protein GP73 is a protein that in ... The protein encoded by this gene is a type II Golgi transmembrane protein. It processes protein synthesized in the rough ... Golgi membrane protein 1 is overexpressed in prostate cancer and lung adenocarcinoma tissue. Blood levels of GP73 are higher in ...

https://en.wikipedia.org/wiki/GOLM1

Pines, Jonathon; Hunter, Tony (1990). "Human cyclin A is adenovirus E1A-associated protein p60 and behaves differently from ... "the discovery of protein kinases that phosphorylate tyrosine residues in proteins, critical for the regulation of a wide ... "Minichromosome Maintenance Proteins Interact with Checkpoint and Recombination Proteins to Promote S-Phase Genome Stability". ... Hunter, Anthony Rex (1969). Aspects of mammalian protein synthesis (PhD thesis). University of Cambridge. OCLC 500479081. "Tony ...

https://en.wikipedia.org/wiki/Anthony_R._Hunter

A divergent MYND domain present in the adenovirus E1A binding protein BS69 was also shown to interact with N-CoR and mediate ... Masselink H, Bernards R (March 2000). "The adenovirus E1A binding protein BS69 is a corepressor of transcription through ... The current evidence suggests that the MYND motif in mammalian proteins constitutes a protein-protein interaction domain that ... protein. ETO has been shown to be a co-repressor recruited by the promyelocytic leukemia zinc finger (PLZF) protein. ...

https://en.wikipedia.org/wiki/MYND_zinc_finger

Masselink H, Bernards R (2000). "The adenovirus E1A binding protein BS69 is a corepressor of transcription through recruitment ... is a protein that in humans is encoded by the NCOR1 gene. NCOR1 is a transcriptional coregulatory protein which contains ... "Both corepressor proteins SMRT and N-CoR exist in large protein complexes containing HDAC3". EMBO J. 19 (16): 4342-50. doi: ... It is a member of the family of nuclear receptor corepressors; the other human protein that is a member of that family is ...

https://en.wikipedia.org/wiki/Nuclear_receptor_co-repressor_1

Pines, Jonathon; Hunter, Tony (1990). "Human cyclin A is adenovirus E1A-associated protein p60 and behaves differently from ... and identified the first link between cyclins and oncoproteins by showing that cyclin A bound to adenovirus E1A, thus linking ... proteolysis coordinates chromosome congression with cytokinesis and mitotic exit by degrading specific proteins at specific ...

https://en.wikipedia.org/wiki/Jonathon_Pines

Cellular Repressor of E1A-stimulated Genes 1) is a protein that in humans is encoded by the CREG1 gene. The adenovirus E1A ... The protein encoded by this gene antagonizes transcriptional activation and cellular transformation by E1A. This protein shares ... Jiang LQ, Wen SJ, Wang HY, Chen LY (2003). "Screening the proteins that interact with calpain in a human heart cDNA library ... 2003). "The Secreted Protein Discovery Initiative (SPDI), a Large-Scale Effort to Identify Novel Human Secreted and ...

https://en.wikipedia.org/wiki/CREG1

... the adenovirus E1A proteins bind to the retinoblastoma gene product". Nature. 334 (6178): 124-9. Bibcode:1988Natur.334..124W. ... Biol 16: 13-47). A.D. Hershey and Martha Chase, "Independent Functions of Viral Protein and Nucleic Acid in Growth of ... a highly conserved protein complex that recognizes and binds to specific DNA sequences, marking starting points for replication ... the small subset of protein-coding genes within the much larger genome-now a mainstay of identifying genetic mutations in ...

https://en.wikipedia.org/wiki/Cold_Spring_Harbor_Laboratory

E1A adenovirus protein, and S-HDAg (hepatitis delta virus small delta antigen). p300/CBP have also been observed to acetylate β ... and E1A adenovirus protein. It can also autoacetylate, which facilitates intramolecular interactions with its bromodomain that ... structural proteins, polyamines, and proteins involved in nuclear import. Acetylation of these proteins can alter their ability ... Histones comprise the protein portion of chromatin. There are five different histone proteins: H1, H2A, H2B, H3, and H4. A core ...

https://en.wikipedia.org/wiki/Histone_acetyltransferase

pRb is one of the targets of the oncogenic human papilloma virus protein E7, and human adenovirus protein E1A. By binding to ... Most E2F have a pocket protein binding domain. Pocket proteins such as pRB and related proteins p107 and p130, can bind to E2F ... Homo sapiens E2F1 mRNA or E2F1 protein sequences from NCBI protein and nucleotide database. X-ray crystallographic analysis has ... Activator E2F proteins can then transcribe S phase promoting genes. In REF52 cells, overexpression of activator E2F1 is able to ...

https://en.wikipedia.org/wiki/E2F

The adenovirus E1B protein (55K) prevents p53 from regulating genes by binding to the site on p53 which binds to the genome. In ... The adenovirus early region 1A (E1A) is an oncoprotein which binds to Rb and can stimulate transcription and transform cells. ... HPV instead degrades p53: the HPV protein E6 binds to a cellular protein called the E6-associated protein (E6-AP, also known as ... DNA oncoviruses typically impair two families of tumor suppressor proteins: tumor proteins p53 and the retinoblastoma proteins ...

https://en.wikipedia.org/wiki/Oncovirus

"Isolation of a cDNA encoding the adenovirus E1A enhancer binding protein: a new human member of the ets oncogene family". ... "Entrez Gene: ETV4 Ets variant gene 4 (E1A enhancer binding protein, E1AF)". "ETV4 Gene - GeneCards , ETV4 Protein , ETV4 ... encoding the adenovirus E1A enhancer-binding protein, in extraosseous Ewing's sarcoma". Biochemical and Biophysical Research ... ETV4+protein,+human at the US National Library of Medicine Medical Subject Headings (MeSH) Overview of all the structural ...

https://en.wikipedia.org/wiki/ETV4

... the Adenovirus protein E1A. Pathogenic bacteria also mimic host motifs (as well as having their own motifs), however, not to ... linear motifs or minimotifs are short stretches of protein sequence that mediate protein-protein interaction. The first ... Linear motif mediated protein-protein interactions have shown promise in recent years as novel drug targets. Success stories ... Beuming T, Skrabanek L, Niv MY, Mukherjee P, Weinstein H (March 2005). "PDZBase: a protein-protein interaction database for PDZ ...

https://en.wikipedia.org/wiki/Short_linear_motif

The CtBP1 protein was originally identified as a human protein that bound a PLDLS motif in the C-terminus of adenovirus E1A ... 1993). "A region in the C-terminus of adenovirus 2/5 E1a protein is required for association with a cellular phosphoprotein and ... 1998). "Interaction between a cellular protein that binds to the C-terminal region of adenovirus E1A (CtBP) and a novel ... "Interaction between a cellular protein that binds to the C-terminal region of adenovirus E1A (CtBP) and a novel cellular ...

https://en.wikipedia.org/wiki/CTBP1

... and relief of repression by adenovirus E1A protein". Cell. 67 (2): 377-88. doi:10.1016/0092-8674(91)90189-6. PMID 1655281. ... Furthermore, SINEs frequently contain motifs for YY1 polycomb proteins. YY1 is a zinc-finger protein that acts as a ... Thereafter, one of the strands is incorporated into a multi-protein RNA-induced silencing complex (RISC). Among these proteins ... encodes a protein which binds to RNA and acts as a chaperone to facilitate and maintain the LINE protein-RNA complex structure ...

https://en.wikipedia.org/wiki/Short_interspersed_nuclear_element

... a zinc-finger protein that shares an epitope with the adenovirus E1A protein". Proceedings of the National Academy of Sciences ... PR domain zinc finger protein 2 is a protein that in humans is encoded by the PRDM2 gene. This tumor suppressor gene is a ... It encodes a zinc finger protein that can bind to retinoblastoma protein, estrogen receptor, and the TPA-responsive element ( ... PRDM2+protein,+human at the US National Library of Medicine Medical Subject Headings (MeSH) This article incorporates text from ...

https://en.wikipedia.org/wiki/PRDM2

The protein is differentially regulated by E1A-induced phosphorylation. The full-length gene product represses transcription ... Fernandes ER, Rooney RJ (1997). "The adenovirus E1A-regulated transcription factor E4F is generated from the human homolog of ... Transcription factor E4F1 is a protein that in humans is encoded by the E4F1 gene. The zinc finger protein encoded by this gene ... "Chromosomal location and tissue expression of the gene encoding the adenovirus E1A-regulated transcription factor E4F in humans ...

https://en.wikipedia.org/wiki/E4F1

Li Y, Graham C, Lacy S, Duncan AM, Whyte P (1994). "The adenovirus E1A-associated 130-kD protein is encoded by a member of the ... Li Y, Graham C, Lacy S, Duncan AM, Whyte P (December 1993). "The adenovirus E1A-associated 130-kD protein is encoded by a ... This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to ... G1/S-specific cyclin-E1 is a protein that in humans is encoded by the CCNE1 gene. The protein encoded by this gene belongs to ...

https://en.wikipedia.org/wiki/Cyclin_E1

"The carboxy-terminal region of adenovirus E1A activates transcription through targeting of a C-terminal binding protein-histone ... Retinoblastoma protein, Retinoblastoma-like protein 1, Retinoblastoma-like protein 2, SAP30, SATB1, SIN3A, SIN3B, SPEN, SUDS3, ... Yao YL, Yang WM (October 2003). "The metastasis-associated proteins 1 and 2 form distinct protein complexes with histone ... the polycomb group proteins HPC2 and BMI-1, and the corepressor C-terminal-binding protein". Proceedings of the National ...

https://en.wikipedia.org/wiki/HDAC1

... replication of the adenovirus genome can occur. A terminal protein that is covalently bound to the 5' end of the adenovirus ... E1A (binds Retinoblastoma tumor suppressor protein) has been found to immortalize primary cells in vitro allowing E1B (binds ... Adenoviruses Stanford University-Adenoviruses Adenoviruses General Concepts General information on Adenovirus DNA virus ... Tupaia adenovirus (TAV) (tree shrew adenovirus 1) has been isolated from tree shrews. Otarine adenovirus 1 has been isolated ...

https://en.wikipedia.org/wiki/Adenoviridae

"Adenovirus E1A, simian virus 40 tumor antigen, and human papillomavirus E7 protein share the capacity to disrupt the ... "A family of proteins structurally and functionally related to the E6-AP ubiquitin-protein ligase". Proceedings of the National ... 1990 Huibregtse, J.M.; Scheffner, M.; Howley, P.M. (1991). "A cellular protein mediates association of p53 with the E6 ... 1989 Werness, B.; Levine, A.; Howley, P. (1990). "Association of human papillomavirus types 16 and 18 E6 proteins with p53". ...

https://en.wikipedia.org/wiki/Peter_M._Howley

Interestingly, adenovirus has an E1A protein to induce apoptosis by initiating the cell cycle, and an E1B protein to block the ... White E (1998). "Regulation of Apoptosis by Adenovirus E1A and E1B Oncogenes". Seminars in Virology. 8 (6): 505-513. doi: ... proteins, DNA and/or RNA. Namely, viral proteins of herpes simplex virus can degrade host DNA and inhibit host cell DNA ... In most cases, tropism is determined by the ability of the viral surface proteins to fuse or bind to surface receptors of ...

https://en.wikipedia.org/wiki/Viral_pathogenesis

"The retinoblastoma protein binds to RIZ, a zinc-finger protein that shares an epitope with the adenovirus E1A protein". Proc. ... The retinoblastoma protein (protein name abbreviated pRb; gene name abbreviated RB or RB1) is a tumor suppressor protein that ... protein binding. • androgen receptor binding. • identical protein binding. • enzyme binding. • ubiquitin protein ligase binding ... protein localization to chromosome, centromeric region. • cell cycle. • striated muscle cell differentiation. • Ras protein ...

https://en.m.wikipedia.org/wiki/Retinoblastoma_protein

The adenovirus E1B protein (55K) prevents p53 from regulating genes by binding to the site on p53 which binds to the genome.[32 ... The adenovirus early region 1A (E1A) is an oncoprotein which binds to Rb and can stimulate transcription and transform cells.[ ... DNA oncoviruses typically impair two families of tumor suppressor proteins: tumor proteins p53 and the retinoblastoma proteins ... the HPV protein E6 binds to a cellular protein called the E6-associated protein (E6-AP, also known as UBE3A), forming a complex ...

https://en.wikipedia.org/wiki/Tumor_viruses

... and adenovirus E1A-associated p33 kinase». Nature 353 (6340): 174-7. PMID 1653904. doi:10.1038/353174a0. ... de 1999). «Regulation of cyclin A-Cdk2 by SCF component Skp1 and F-box protein Skp2». Mol. Cell. Biol. (UNITED STATES) 19 (1): ... de 2000). «p12(DOC-1) is a novel cyclin-dependent kinase 2-associated protein». Mol. Cell. Biol. (UNITED STATES) 20 (17): 6300- ... de 1997). «BRCA1 proteins are transported to the nucleus in the absence of serum and splice variants BRCA1a, BRCA1b are ...

https://es.wikipedia.org/wiki/Cdk2

... replication of the adenovirus genome can occur. A terminal protein that is covalently bound to the 5' end of the adenovirus ... E1A (binds Retinoblastoma tumor suppressor protein) has been found to immortalize primary cells in vitro allowing E1B (binds ... Adenoviruses are also known to cause respiratory infections in horses, cattle, pigs, sheep, and goats. Equine adenovirus 1 can ... The late phase of the adenovirus lifecycle is focused on producing sufficient quantities of structural protein to pack all the ...

https://en.m.wikipedia.org/wiki/Adenoviridae

"Oncogene expression cloning by retroviral transduction of adenovirus E1A-immortalized rat kidney RK3E cells: transformation of ... are dependent on acidic amino acid residues and protein-protein interaction". Nucleic Acids Research. 28 (5): 1106-13. doi: ... are dependent on acidic amino acid residues and protein-protein interaction". Nucleic Acids Research. 28 (5): 1106-13. doi: ... are dependent on acidic amino acid residues and protein-protein interaction". Nucleic Acids Research. 28 (5): 1106-13. doi: ...

https://en.wikipedia.org/wiki/KLF4

"Interaction between a cellular protein that binds to the C-terminal region of adenovirus E1A (CtBP) and a novel cellular ... Retinoblastoma-binding protein 8 is a protein that in humans is encoded by the RBBP8 gene. The protein encoded by this gene is ... "Interaction between a cellular protein that binds to the C-terminal region of adenovirus E1A (CtBP) and a novel cellular ... It is found among several proteins that bind directly to retinoblastoma protein, which regulates cell proliferation. This ...

https://en.wikipedia.org/wiki/RBBP8

... adenovirus e1a proteins MeSH D12.776.964.700.045.050.110 - adenovirus e1b proteins MeSH D12.776.964.700.045.060 - adenovirus e2 ... adenovirus E1 proteins MeSH D12.776.624.664.520.045.050.100 - adenovirus E1A proteins MeSH D12.776.624.664.520.045.050.110 - ... adenovirus e2 proteins MeSH D12.776.624.664.520.045.070 - adenovirus e3 proteins MeSH D12.776.624.664.520.045.080 - adenovirus ... adenovirus e3 proteins MeSH D12.776.964.700.045.080 - adenovirus e4 proteins MeSH D12.776.964.700.750.320 - fusion proteins, ...

https://en.wikipedia.org/wiki/List_of_MeSH_codes_(D12.776)

Control protein E1A activates transcription of a number of viral genes as well as genes of the host cell. Control protein E1B ... The functions of many adenovirus proteins are known: Structural proteins include capsid proteins II (hexon), III (penton base ... Adenoviruses. Fundamentals of Molecular Virology (2 ed.). John Wiley & Sons, Inc. "Protein Details for Human adenovirus E". ... and the terminal protein TP. Encapsidation proteins IVa2, 52K, and L1, and hexon assembly protein 100K are involved in assembly ...

https://en.wikipedia.org/wiki/Adenovirus_genome

Li Y, Graham C, Lacy S, Duncan AM, Whyte P (Dec 1993). "The adenovirus E1A-associated 130-kD protein is encoded by a member of ... Li Y, Graham C, Lacy S, Duncan AM, Whyte P (Dec 1993). "The adenovirus E1A-associated 130-kD protein is encoded by a member of ... Retinoblastoma-like protein 2 is a protein that in humans is encoded by the RBL2 gene. Retinoblastoma-like protein 2 has been ... "The HMG-box transcription factor HBP1 is targeted by the pocket proteins and E1A". Oncogene. 14 (22): 2721-8. doi:10.1038/sj. ...

https://en.wikipedia.org/wiki/Retinoblastoma-like_protein_2

... whose presence correlates with activation of RNA polymerase III-mediated transcription by adenovirus E1A expression and serum ... "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173-8. Bibcode:2005Natur. ... GTF3C2+protein,+human at the US National Library of Medicine Medical Subject Headings (MeSH) FactorBook TFIIIC-110 This article ... General transcription factor 3C polypeptide 2 is a protein that in humans is encoded by the GTF3C2 gene. GTF3C2 has been shown ...

https://en.wikipedia.org/wiki/GTF3C2

... cloning and characterization of a cellular phosphoprotein that interacts with a conserved C-terminal domain of adenovirus E1A ... C-terminal-binding protein 2 also known as CtBP2 is a protein that in humans is encoded by the CTBP2 gene. The CtBPs - CtBP1 ... "The LIM protein FHL3 binds basic Krüppel-like factor/Krüppel-like factor 3 and its co-repressor C-terminal-binding protein 2". ... "C-Terminal binding protein is a transcriptional repressor that interacts with a specific class of vertebrate Polycomb proteins ...

https://en.wikipedia.org/wiki/CTBP2

Tsai LH, Harlow E, Meyerson M (September 1991). "Isolation of the human cdk2 gene that encodes the cyclin A- and adenovirus E1A ... The protein encoded by this gene is a member of the cyclin-dependent kinase family of Ser/Thr protein kinases. This protein ... This increases the synthesis of histone proteins (the major protein component of chromatin), and subsequently supports the DNA ... where cells make proteins necessary for mitosis and replicate their DNA. This protein associates with and is regulated by the ...

https://en.wikipedia.org/wiki/Cyclin-dependent_kinase_2

"Regulation of the 26S proteasome by adenovirus E1A". EMBO J. 19 (17): 4759-73. doi:10.1093/emboj/19.17.4759. PMC 302057. PMID ... To recognize protein as designated substrate, 19S complex has subunits that are capable to recognize proteins with a special ... Accordingly, misfolded proteins and damaged protein need to be continuously removed to recycle amino acids for new synthesis; ... The 19S regulatory particles can recognize ubiquitin-labeled protein as degradation substrate, unfold the protein to linear, ...

https://en.wikipedia.org/wiki/PSMC1

SV40 large TAg, other polyomavirus large T antigens, adenovirus E1a proteins, and oncogenic human papillomavirus E7 proteins ... Protein-protein interactions between T-antigen and DNA polymerase-alpha directly stimulate replication of the virus genome. T- ... "The binding domain structure of retinoblastoma-binding proteins". Protein Science. 2 (2): 155-64. doi:10.1002/pro.5560020204. ... The SV40 large T-antigen has been used as a model protein to study nuclear localization signals (NLSs). It is imported into the ...

https://en.wikipedia.org/wiki/SV40_large_T_antigen

... the host cell must be induced into S phase by viral proteins interfering with cell cycle proteins. The adenoviral E1A gene is ... and the knob domain of the adenovirus coat protein trimer. CAR is necessary for adenovirus infection. Although expressed widely ... A conditionally replicative adenovirus (CRAd) with a 24 base pair deletion in the retinoblastoma-binding domain of the E1A ... E1B proteins produced by adenoviruses protect the infected cell by binding to and degrading the p53 transcription factors, ...

https://en.wikipedia.org/wiki/Oncolytic_adenovirus

The adenovirus E1a protein stimulates transcription of a wide variety of viral and cellular genes. It is shown here that E1a ... Transcription activation by the adenovirus E1a protein.. Lillie JW1, Green MR. ... perhaps by interacting with a DNA-bound protein, and the other, an activating region, enables the bound activator to stimulate ... has the two functions characteristic of a typical cellular activator: one direct E1a to the promoter, ...

https://www.ncbi.nlm.nih.gov/pubmed/2521923?dopt=Abstract

The adenovirus E1A 243R oncoprotein encodes a potent transcription-repression function within the N-terminal 80 amino acids. ... Our proposed model of E1A repression predicts that E1A interacts with im... ... Adenovirus E1A proteins are closely associated with chromatin in productively infected and transformed cells. *Green M ... Analyses of chromatin from HeLa cells infected with adenovirus vectors expressing E1A 243R protein with deletions in different ...

https://www.mendeley.com/papers/adenovirus-e1a-proteins-closely-associated-chromatin-productively-infected-transformed-cells/

E1A protein has both transforming and trans-activating activities. Induces the disassembly of the E2F1 transcription factor ... The tumorigenesis-restraining activity of E1A may be related to the disruption of the host CtBP-CtIP complex through the CtBP ... Interaction with RBX1 and CUL1 inhibits ubiquitination of the proteins targeted by SCF(FBXW7) ubiquitin ligase complex, and may ... would arrest the cell proliferation and direct its death but this effect is counteracted by the viral protein E1B-55K. ...

https://www.uniprot.org/uniprot/P03259

Adenovirus E1A proteins stimulate inositol phospholipid metabolism in PC12 cells.. K Shiroki, A Yamakawa, M Shibata, T Takenawa ... Adenovirus E1A proteins stimulate inositol phospholipid metabolism in PC12 cells.. K Shiroki, A Yamakawa, M Shibata, T Takenawa ... Adenovirus E1A proteins stimulate inositol phospholipid metabolism in PC12 cells.. K Shiroki, A Yamakawa, M Shibata, T Takenawa ... Adenovirus E1A proteins stimulate inositol phospholipid metabolism in PC12 cells. Message Subject (Your Name) has forwarded a ...

https://jvi.asm.org/content/66/10/6093

A novel chimera gene between EWS and E1A-F, encoding the adenovirus E1A enhancer-binding protein, in extraosseous Ewings ... encoding the adenovirus E1A enhancer-binding protein. Since E1A-F is known to activate matrix metalloproteinase genes, the ... In this study, we identified a new chimera gene between the transactivation domain of EWS and E1A-F, ...

https://pubmed.ncbi.nlm.nih.gov/8605035/?dopt=Abstract

... the 243R protein, for immortalization, ras cooperative transformation, tumorigenesis and metastasis. Compared with wild-type ... We have examined a series of small deletion mutants within exon 2 of the adenovirus 2/5 E1A oncogene product, ... This fusion protein was used to purify cellular proteins that bind to the C-terminal region of E1a. A 48 kDa cellular protein ... protein. It also co-immunoprecipitated specifically with E1a. Analysis of a panel of GST-E1a C-terminal mutant proteins ...

https://pubmed.ncbi.nlm.nih.gov/8440238/

Molecular cloning and functional analysis of the adenovirus E1A-associated 300-kD protein (p300) reveals a protein with ... Recruitment of CBP/p300, TATA-Binding Protein, and S8 to Distinct Regions at the N Terminus of Adenovirus E1A. Mozhgan Rasti, ... Amino acids 1-29 of the adenovirus serotypes 12 and 2 E1A proteins interact with rap30 [TF(II)F] and TBP in vitro. Virus Res. ... The adenovirus E1A repression domain disrupts the interaction between the TATA binding protein and the TATA box in a manner ...

https://jvi.asm.org/content/79/9/5594?ijkey=47910da2de359956f1155f3f85fc47f888e2c619&keytype2=tf_ipsecsha

... family proteins through distinct domains in the E1A N-terminal region. The C-terminal region of E1A suppresses E1A/Ras co- ... Highlights: • Adenovirus E1A C-terminal region suppresses E1A/Ras co-transformation. • This E1A region binds with FOXK, DYRK1/ ... Adenovirus E1A induces cell proliferation, oncogenic transformation and promotes viral replication through interaction with ... Title: Interaction of CtBP with adenovirus E1A suppresses immortalization of primary epithelial cells and enhances virus ...

https://www.osti.gov/scitech/biblio/22436638-interaction-ctbp-adenovirus-e1a-suppresses-immortalization-primary-epithelial-cells-enhances-virus-replication-during-productive-infection

We suggest that the ability of E1A, the simian virus 40 large tumor antigen, and E7 to dissociate the E2F-pRb complex may be a ... We now show that the E7 protein and the simian virus 40 large tumor antigen can dissociate the E2F-pRb complex, dependent on ... These sequences are also required for these proteins to bind to the retinoblastoma gene product (pRb). Recent experiments have ... E7 protein share a short amino acid sequence that constitutes a domain required for the transforming activity of these proteins ...

https://www.semanticscholar.org/paper/Adenovirus-E1A%2C-simian-virus-40-tumor-antigen%2C-and-Chellappan-Kraus/a23bd7f8d7986d97223474dbd24d77e8bbdb8285

A truncated EIA protein, in which 169 carboxyl terminal residues of the 289-amino acid E1A 13 S mRNA product are deleted, was ... The E1A proteins stimulated cellular DNA synthesis in mouse Swiss 3T3 cells, when microinjected either individually or in ... Our results provide evidence that E1A proteins can function, independent of other viral functions, in the stimulation of ... The present results are consistent with the E1A gene products acting to modulate the expression of the cellular genes which ...

https://academic.naver.com/article.naver?doc_id=324659828

... although the 135 E1A protein binds more efficiently than the 12S E1A protein. Two domains on the E1A proteins interact with YY1 ... although the 135 E1A protein binds more efficiently than the 12S E1A protein. Two domains on the E1A proteins interact with YY1 ... although the 135 E1A protein binds more efficiently than the 12S E1A protein. Two domains on the E1A proteins interact with YY1 ... although the 135 E1A protein binds more efficiently than the 12S E1A protein. Two domains on the E1A proteins interact with YY1 ...

https://collaborate.princeton.edu/en/publications/adenovirus-e1a-proteins-interact-with-the-cellular-yy1-transcript

Adenovirus E1A protein activates transcription of the E1A gene subsequent to transcription complex formation. Journal of ... Adenovirus E1A protein activates transcription of the E1A gene subsequent to transcription complex formation. / Schaack, J.; ... Schaack, J., Logan, J., Vakalopoulou, E., & Shenk, T. (1991). Adenovirus E1A protein activates transcription of the E1A gene ... Schaack, J. ; Logan, J. ; Vakalopoulou, E. ; Shenk, T. / Adenovirus E1A protein activates transcription of the E1A gene ...

https://collaborate.princeton.edu/en/publications/adenovirus-e1a-protein-activates-transcription-of-the-e1a-gene-su

Adenovirus E1A Proteins. Grant support. *R01 CA90784-01A1/CA/NCI NIH HHS/United States ... We also observed that the virus without any promoter for the E1a gene could still express leaky levels of E1A which can lead to ... one without any promoter controlling the E1a gene and two vectors with the E1a gene being controlled by either its endogenous ... Adenoviral E1a expression levels affect virus-selective replication in human cancer cells.. Zheng X1, Rao XM, Snodgrass C, Wang ...

https://www.ncbi.nlm.nih.gov/pubmed/16222119?dopt=Abstract

METHODS TO OBTAIN RECOMBINANT PROTEINS WITH INCREASED SIALYLATION FROM CELLS THAT EXPRESS ADENOVIRUS E1A PROTEIN. ... Thus, two aspects appear relevant for increasing sialylation of produced proteins in cells that express adenovirus El A protein ... INVENTION Methods to obtain recombinant proteins with increased sialylation from cells that express adenovirus El A protein, ... The expression of E1A influences the glycosylation of proteins produced in such cells (WO 03/038100).. N-linked glycans are ...

http://www.allindianpatents.com/patents/250691-methods-to-obtain-recombinant-proteins-with-increased-sialylation-from-cells-that-express-adenovirus-e1a-protein

SAHA downregulated Bcl-XL and upregulated proapoptotic BH3-only protein Bim, whose expression was further enhanced by E1A in ... Adenovirus 5 early region 1A (E1A) has been shown to exhibit high tumor suppressor activity, and gene therapy using E1A has ... Here, we showed that proapoptotic activity of HDACi was robustly enhanced by E1A in multiple cancer cells, but not in normal ... Together, our results provide not only a novel insight into the mechanisms underlying anti-tumor activity of E1A, but also a ...

https://www.nature.com/articles/onc2010295?error=cookies_not_supported&code=e4883e19-2355-4560-a96b-e8d7161d8c97

... immunohistochemical detection of E1A. Tumor cells that expressed E1A protein were detected as brown spots (sections were ... replication-competent adenovirus vectors overexpressing the adenovirus death protein. J. Virol., 74: 6147-6155, 2000. ... E1A, E1B Double-restricted Adenovirus for Oncolytic Gene Therapy of Gallbladder Cancer. Kuniaki Fukuda, Masato Abei, Hideyo ... A mutant Ad5 E1A gene fragment containing a STGHE (SXGXE) mutation at the LTCHE (LXCXE) Rb-binding pocket motif in Ad5 E1A exon ...

https://cancerres.aacrjournals.org/content/63/15/4434

The F-box protein is one of the four components of the SCF (SKp1, Cullin, F-box protein) complex, which mediates ubiquitination ... On the other hand, F-box proteins can be used in the defense response by the host. This review describes the role of F-box ... of proteins targeted for degradation by the proteasome, playing an essential role in many cellular processes. Several ... proteins and the use of the ubiquitin-proteasome system in virus-host interactions. ...

http://www.mdpi.com/1422-0067/14/2/4030/htm

Interaction of Adenovirus E1A with the HHV8 Promoter of Latent Genes: E1A Proteins are Able to Activate the HHV-8 LANAp in MV3 ... Adenoviral (Ad) E1A proteins regulate the activity of cellular and viral promoters/enhancers and transcription factors and can ... In summary, we report the activation of the LANAp by E1A as a novel interaction of E1A with a viral promoter. These data may ... and LANA proteins required for latent type infection is regulated by E1A. Transfection experiments in MV3 melanoma cells ...

https://openvirologyjournal.com/VOLUME/2/PAGE/61/ABSTRACT/

Identification of specific adenovirus E1A N-terminal residues critical to the binding of cellular proteins and to the control ... Identification of specific adenovirus E1A N-terminal residues critical to the binding of cellular proteins and to the control ... Identification of specific adenovirus E1A N-terminal residues critical to the binding of cellular proteins and to the control ... Identification of specific adenovirus E1A N-terminal residues critical to the binding of cellular proteins and to the control ...

https://www.researchwithrutgers.com/en/publications/identification-of-specific-adenovirus-e1a-n-terminal-residues-cri

... an adenovirus E1A-binding protein, and maps to human chromosome 21q21.3. ... A novel C-terminal binding protein (CTBP2) is closely related to CTBP1, ... A novel C-terminal binding protein (CTBP2) is closely related to CTBP1, an adenovirus E1A-binding protein, and maps to human ... A region of the C-terminus of adenovirus type 2/5 E1A protein has been associated with negative modulation of tumorigenicity, ...

https://scholars.duke.edu/display/pub779521

T1 - Two domains of p53 interact with the TATA-binding protein, and the adenovirus 13S E1A protein disrupts the association, ... title = "Two domains of p53 interact with the TATA-binding protein, and the adenovirus 13S E1A protein disrupts the association ... Two domains of p53 interact with the TATA-binding protein, and the adenovirus 13S E1A protein disrupts the association, ... Two domains of p53 interact with the TATA-binding protein, and the adenovirus 13S E1A protein disrupts the association, ...

https://www.scholars.northwestern.edu/en/publications/two-domains-of-p53-interact-with-the-tata-binding-protein-and-the

T1 - A novel chimera gene between EWS and E1A-F, encoding the adenovirus E1A enhancer-binding protein, in extraosseous Ewings ... A novel chimera gene between EWS and E1A-F, encoding the adenovirus E1A enhancer-binding protein, in extraosseous Ewings ... A novel chimera gene between EWS and E1A-F, encoding the adenovirus E1A enhancer-binding protein, in extraosseous Ewings ... A novel chimera gene between EWS and E1A-F, encoding the adenovirus E1A enhancer-binding protein, in extraosseous Ewings ...

https://keio.pure.elsevier.com/en/publications/a-novel-chimera-gene-between-ews-and-e1a-f-encoding-the-adenoviru

Human being adenovirus type 12 (Advertisement12) E1A proteins (E1A-12) may be. Published on September 8, 2018. Author ... Human being adenovirus type 12 (Advertisement12) E1A proteins (E1A-12) may be the crucial determinant of viral tumorigenesis. ... E1A protein of nontumorigenic adenoviruses, such as for example Advertisement5, cannot suppress MHC-I appearance, Quetiapine ... These outcomes demonstrate that E1A-12 particularly stops NF-B from getting phosphorylated by PKAc. Individual adenovirus type ...

http://www.globaltechbiz.com/2018/09/08/human-being-adenovirus-type-12-advertisement12-e1a-proteins-e1a-12-may-be/

The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex ... but which include the SWI/SNF related protein SRCAP. The NuA4 complex interacts with MYC and the adenovirus E1A protein. EP400 ... Interacts with human adenovirus 5 E1A protein; this interaction stabilizes MYC (PubMed:18413597). UniProt ... This protein in other organisms (by gene name): Q96L91 - Homo sapiens 0 * Q6P2F5 - Homo sapiens no matching PDB entries ...

http://www.rcsb.org/pdb/protein/Q96L91

The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex ... Isoform early E1A 32 kDa protein and isoform early E1A 26 kDa protein interact (via N-terminus) with CUL1 and E3 ubiquitin ... E1A protein has both transforming and trans-activating activities. Induces the disassembly of the E2F1 transcription factor ... Protein disorder predictions are based on JRONN (Troshin, P. and Barton, G. J. unpublished), a Java implementation of RONN * ...

http://www.rcsb.org/pdb/protein/P03255

... we transfected primary BRK cells with plasmids encoding the IE1 and IE2 proteins as well as the adenovirus E1A protein. IE1 or ... and the E1A protein was detected throughout the time course in cells transformed by E1A/IE1/IE2 or by E1A/E1B. Even though the ... Since mutant p53 alleles are known to cooperate with the adenovirus E1A protein to transform rodent cells (32), we tested ... In cell lines derived by transfection with E1A plus E1B, both E1A (Fig. 2C) and E1B (data not shown) proteins are readily ...

http://www.pnas.org/content/94/7/3341.full

Arched Isoelectric focusing Method for the Study of Proteins in the Cat Aqueous Humor* / p49 (0114.jp2) ... Molecular mechanism of tumorigenesis in transgenic mice carrying E1A and E1B genes of human adenovirus * * 杉山, 文博スギヤマ, フミヒロ ... Neuroectodermal Tumors Expressing c-,L-,and N-myc in Transgenic Mice That Carry the E1A/E1B Gene of Human Adenovirus Type 12 ... 1.Production of transgenic mice carrying human renin promoter/E1A and E1B genes of human adenovirus type 12 / p12 (0020.jp2) ...

https://ci.nii.ac.jp/naid/500000130913

Functional and Sequence Similarities Between HPV16 E7 and Adenovirus E1A W. C. Phelps, C. L. Yee, K. Münger, P. M. Howley ... DNA RNA antigen cancer gene hepatitis hepatitis B liver molecular biology protein proteins tumor tumorigenesis virology virus ... The Transforming Protein of Simian Virus 40: Large T Antigen and Its Interaction with the Cellular Protein p 53. * Front Matter ... SV40 Large T Antigen Induces a Protein Kinase Responsible for Phosphorylation of the Cellular Protein p53 ...

https://link.springer.com/book/10.1007%2F978-3-642-74578-2

proteins involved in adenovirus and BK virus (DNA tumor virus) adenovirus - E1A and E1B. BK - Large T ...

https://www.brainscape.com/flashcards/viral-oncogenesis-4106012/packs/6074077

The E1A gene of species C human adenovirus is an intensely investigated model viral oncogene that immortalizes primary cells ... Adenovirus E1A Proteins / metabolism*. Humans. Oncogene Proteins, Viral / metabolism*. Tumor Suppressor Proteins / metabolism. ... 0/Adenovirus E1A Proteins; 0/Oncogene Proteins, Viral; 0/Tumor Suppressor Proteins; 0/Virulence Factors ... The E1A gene of species C human adenovirus is an intensely investigated model viral oncogene that immortalizes primary cells ...

http://www.biomedsearch.com/nih/Opposing-oncogenic-activities-small-DNA/21330137.html

The adenovirus E1a protein stimulates transcription of a wide variety of viral and cellular genes. (nih.gov)
Since E1A-F is known to activate matrix metalloproteinase genes, the chimera gene may possibly be involved in tumor progression and could be a novel tumor marker for Ewing's sarcoma/PNET. (nih.gov)
The protein products of 12S and 13S differ only in the presence of conserved region 3 (CR3), which functions to transactivate a number of cellular and viral genes ( 22 ). (asm.org)
AdE1A can cooperate with AdE1B or activated ras genes to transform both human ( 6 ) and rodent ( 33 ) cells by targeting a limited number of cellular proteins through the N-terminal region, CR1, CR2, and CR4 ( 3 , 18 , 26 , 44 ). (asm.org)
The present results are consistent with the E1A gene products acting to modulate the expression of the cellular genes which control cell cycle progression into S phase. (naver.com)
The mechanism of transcriptional activation of the adenovirus E1A and E3 genes by E1A protein during infection was examined by using transcription-competition assays. (princeton.edu)
One of the promising strategies for targeting replication of oncolytic adenovirus in tumor cells is to regulate the expression of essential viral genes such as E1a by using tumor- or tissue-specific promoters that are preferentially active in cancer cells. (nih.gov)
Significantly, the E1A-12 oncoprotein mediates reduced expression of main histocompatibility complex course I (MHC-I) antigens in the cell surface area Quetiapine manufacture by inhibiting the transcription of most course I genes (1, 7, 10, 29, 33). (globaltechbiz.com)
Here we show that the HCMV immediate early 1 and 2 genes can cooperate with the adenovirus E1A gene to generate transformed foci of primary baby rat kidney cells. (pnas.org)
We find that the HCMV immediate early proteins are mutagenic, and we propose that HCMV has the potential to contribute to oncogenesis through a "hit-and-run" mechanism, by inducing mutations in cellular genes. (pnas.org)
BRK cells, which have been used extensively to assay the transforming activity of adenovirus oncogenes ( 21 , 22 ), were used to test the oncogenic potential of the HCMV immediate early genes. (pnas.org)
p53 and pRB are well-established tumor suppressor proteins, and their corresponding genes are commonly lost or mutated in human cancer. (asm.org)
For example, cancer cells in which the tumor suppressor proteins p53 or pRb are inactivated were reported to be permissive for efficient replication of adenoviruses that are mutated in the E1B55K or E1A genes that encode p53- or pRb-inactivating proteins, respectively ( 7 -9 ). (aacrjournals.org)
The family members share a 300-aa Rel homology region that is responsible for protein dimerization, nuclear localization, and DNA binding to κB elements in the enhancer regions of target genes. (pnas.org)
Furthermore, they produced new transformed lines by introducing genes encoding E6, E7, E1A, or T into normal mouse embryonic fibroblasts. (virology.ws)
Cytoplasmic DNA is detected in cells by an enzyme called cGAS (cyclic guanosine monophosphate-adenosine monophosphate synthase) together with an adaptor protein known as STING (stimulator of interferon genes). (virology.ws)
The proteins encoded by Myb genes bind to DNA and regulate the expression of other genes that control cell division, differentiation, and cell death. (stanford.edu)
Results: Double-regulated duplicative adenovirus AdTPHre-hEndo genes were successfully constructed. (lu.se)
1996 ). Interferon-modulated expression of genes encoding the nuclear-dot-associated proteins Sp100 and promyelocytic leukemia protein (PML). (biologists.org)
Our laboratory studies how these viruses infect normal human cells and the consequences of the subsequent interactions between the viral oncogenes and the cellular DNA, genes and proteins. (dana-farber.org)
The general principle appears to be that two or more early proteins co-operate to give a transforming phenotype.The most confusing thing is that in most cases, all or part of the Papilloma genome including the putative "transforming genes" is maintained in the tumour cells, whereas in other cases, the virus DNA may be lost after transformation. (kenyon.edu)
Both genes give rise to proteins that have ( a ) entirely novel functions and ( b ) p53-related functions. (aacrjournals.org)
All genes are expressed as two major types: full-length proteins containing the TA domain and ΔN proteins missing the TA domain. (aacrjournals.org)
Berkner, "Development of Adenovirus Vectors for the Expression of Heterologous Genes," BioTechniques 6(7): 616-629, 1988. (patentgenius.com)

This E1A region binds with FOXK, DYRK1/HAN11 and CtBP cellular protein complexes. (osti.gov)
Clink, a nanovirus-encoded protein, binds both pRB and SKP1. (semanticscholar.org)
In this study, using a variety of in vivo and in vitro assays, we demonstrate that both E1A proteins can bind to YY1, although the 135 E1A protein binds more efficiently than the 12S E1A protein. (princeton.edu)
After infecting host human cells, Ad produces an early expression protein, E1A, which binds to pRb to activate E2F. (aacrjournals.org)
The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. (genecards.org)
E1a binds to a key domain of these related proteins (the transcriptional adaptor motif region), functionally inactivating the protein. (aacrjournals.org)
REFERENCE 6 (residues 1 to 445) AUTHORS Holmes M, Turner J, Fox A, Chisholm O, Crossley M and Chong B. TITLE hFOG-2, a novel zinc finger protein, binds the co-repressor mCtBP2 and modulates GATA-mediated activation JOURNAL J. Biol. (genome.jp)
A herpesvirus protein has been identified that binds to STING and blocks interferon responses to cytoplasmic DNA. (virology.ws)
Why not - the same five amino acid sequence that binds cGAS also binds cellular proteins (such as Rb), disrupting their function and leading to uncontrolled cell growth! (virology.ws)
Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. (proteopedia.org)
The recent finding that PIC1/SUMO-1, a small ubiquitin-like protein, is covalently linked to the RanGAP1 protein of the nuclear pore complex and also binds PML in yeast cells led us to determine whether PML is covalently modified by PIC1/SUMO-1 and whether the same is true for Sp100. (rupress.org)
Although its common function is seen as binding and repressing E2F targets, Rb is likely a multifunctional protein as it binds to at least 100 other proteins. (wikipedia.org)
[16] Rb binds and inhibits E2 promoter-binding-protein-dimerization partner (E2F-DP) dimers, which are transcription factors of the E2F family that push the cell into S phase. (wikipedia.org)
This gene encodes a protein that binds to the C-terminus of adenovirus E1A proteins. (thermofisher.com)
Nevertheless, it is clear that most proteins do specify their own import signal and, of these, the vast majority have an NLS that binds to importin-alpha (also termed karyopherin-alpha). (eu.org)
E7-- Transforming protein, binds to pRB/p107. (kenyon.edu)

The C-terminal region of E1A suppresses E1A/Ras co-transformation and interacts with FOXK1/K2, DYRK1A/1B/HAN11 and CtBP1/2 (CtBP) protein complexes. (osti.gov)
The NuA4 complex interacts with MYC and the adenovirus E1A protein. (rcsb.org)
CREB-binding protein (CBP) is a coactivator that interacts with the cAMP-response element binding protein (CREB), a process dependent on the cAMP-dependent protein kinase A and its phosphorylation of CREB ( 21 , 22 ). (pnas.org)
TITLE C-Terminal binding protein is a transcriptional repressor that interacts with a specific class of vertebrate Polycomb proteins JOURNAL Mol. (genome.jp)
The same complex also interacts with proteins of the Rb tumor suppressor family and the E2F transcription factor family. (stanford.edu)
1996 ). PIC1, a novel ubiquitin-like protein which interacts with the PML component of a multiprotein complex that is disrupted in acute promyelocytic leukaemia. (biologists.org)
Importin-alpha is an adaptor protein for importin-beta which interacts with nuclear pore components to effect transport into the nucleus. (eu.org)
Among Harlow's discoveries was the demonstration that the retinoblastoma protein interacts with viral transforming proteins, thereby linking tumor viruses with the cell cycle. (wikipedia.org)

Adenovirus (Ad) E1A expression is essential for Ad replication and Ad-mediated transformation ( 34 ). (asm.org)
Adenovirus E1A induces cell proliferation, oncogenic transformation and promotes viral replication through interaction with p300/CBP, TRRAP/p400 multi-protein complex and the retinoblastoma (pRb) family proteins through distinct domains in the E1A N-terminal region. (osti.gov)
To specifically dissect the role of CtBP interaction with E1A, we engineered a mutation (DL→AS) within the CtBP-binding motif, PLDLS, and investigated the effect of the mutation on immortalization and Ras cooperative transformation of primary cells and viral replication. (osti.gov)
During productive infection, CtBP-E1A interaction enhances viral replication in human cells. (osti.gov)
Between the two CtBP family proteins, CtBP2 appears to restrict viral replication more than CtBP1 in human cells. (osti.gov)
Adenoviral E1a expression levels affect virus-selective replication in human cancer cells. (nih.gov)
In this study, we investigated the effect of E1a expression levels on the virus replication ability in human cells. (nih.gov)
We observed that the CMV promoter-mediated high levels of E1A expression could increase virus replication, resulting in the titers of the E1B55K-mutated virus being even higher than the wild-type virus in some cancer cells. (nih.gov)
The results suggest that whether increased E1A levels would enhance E1B55K-mutated virus replication may be also depended on cellular factors or pathways in cancer cells. (nih.gov)
We also observed that the virus without any promoter for the E1a gene could still express leaky levels of E1A which can lead to viral replication in normal and cancer cells. (nih.gov)
Recent studies have introduced mutant adenoviruses (Ads) with either defective E1B-55kD or mutated E1A, focusing on tumor-specific replication, and the results have been promising. (aacrjournals.org)
Cytoplasmic gene expression and replication, so viral core has to carry all necessary proteins. (brainscape.com)
NAP-1 is a functional homologue of TAF-1 that is required for replication and transcription of the adenovirus genome in a chromatin-like structure. (nature.com)
Many DNA virus replication-related proteins are associated with promyelocytic leukemia protein (PML), a component of nuclear domain 10 (ND10), which has been investigated for its potential involvement in viral replication. (frontiersin.org)
The cells divide, and in the process produce proteins involved in DNA replication, which are then used for viral replication. (virology.ws)
1996 ). Adenovirus replication is coupled with the dynamic properties of the PML nuclear structure. (biologists.org)
The first problem encountered was due to virus gene expression and leakiness in the replication defect of the commonly used defective adenovirus strains. (springer.com)
Here we demonstrate that the tiny RS-repeat is required for localization of the protein to the nucleus and viral replication centers. (diva-portal.org)

We have examined a series of small deletion mutants within exon 2 of the adenovirus 2/5 E1A oncogene product, the 243R protein, for immortalization, ras cooperative transformation, tumorigenesis and metastasis. (nih.gov)
To identify cellular proteins that may associate with the exon 2-coded region of E1A (C-terminal half) and modulate its transformation potential, we constructed a chimeric gene coding for the C-terminal 68 amino acids of E1a fused to bacterial glutathione-S-transferase (GST). (nih.gov)
Analysis of a panel of GST-E1a C-terminal mutant proteins indicates that residues 225-238 are required for the association of E1a and CtBP, suggesting a correlation between the association of CtBP and the immortalization and transformation modulating activities of exon 2. (nih.gov)
The N-terminal region of the adenovirus (Ad) 12S E1A gene product targets several cellular proteins that are essential for the induction of S phase, cellular immortalization, cellular transformation, transcriptional repression, and transcriptional activation. (asm.org)
Finally, we took advantage of the fine-mapping data to ascertain which proteins were targeted during the transformation process. (asm.org)
Consistent with previous studies, CBP/p300 was found to be targeted by AdE1A during this process, although our data suggest that binding to other N-terminal proteins is also important for transformation. (asm.org)
Exon 2, encompassing the whole C-terminal region, suppresses AdE1A/ ras -mediated transformation, primarily through a conserved PXDLS motif that targets the transcriptional corepressor molecule CtBP, and deletion of the PXDLS motif enhances E1A/ ras -mediated transformation ( 4 ). (asm.org)
In contrast, the C-terminal region enhances E1A/E1B-mediated transformation ( 14 ) through the targeting of AdE1A to the nucleus and the binding of CtBP. (asm.org)
Interestingly, however, the AdE1A N-terminal regions from different Ad serotypes appear to bind to a similar set of cellular proteins, presumably to perform similar functions during infection and transformation ( 3 , 18 ). (asm.org)
Our results suggest that CtBP-E1A interaction suppresses immortalization and Ras co-operative transformation of primary rodent epithelial cells without significantly influencing the tumorigenic activities of transformed cells in immunodeficient and immunocompetent animals. (osti.gov)
Adenovirus E1A C-terminal region suppresses E1A/Ras co-transformation. (osti.gov)
We found that E1A-CtBP interaction suppresses immortalization and transformation. (osti.gov)
Adenovirus early region 1A (E1A) oncogene-encoded sequences essential for transformation- and cell growth-regulating activities are localized at the N terminus and in regions of highly conserved amino acid sequence designated conserved regions 1 and 2. (researchwithrutgers.com)
A region of the C-terminus of adenovirus type 2/5 E1A protein has been associated with negative modulation of tumorigenicity, as well as the extent of oncogenic transformation. (duke.edu)
The E1A gene of species C human adenovirus is an intensely investigated model viral oncogene that immortalizes primary cells and mediates oncogenic cell transformation in cooperation with other viral or cellular oncogenes. (biomedsearch.com)
Studies with E1A have led to the unexpected discovery that E1A also suppresses cell transformation and oncogenesis. (biomedsearch.com)
We have previously demonstrated that the N-terminal J domain of TAg affects the RB-related proteins by perturbing the phosphorylation status of p107 and p130 and promoting the degradation of p130 and that this domain is required for transformation of cells that express either p107 or p130. (asm.org)
While p107 and p130 inactivation likely contributes to TAg-mediated transformation (see below), there is little evidence that these proteins are tumor suppressors. (asm.org)
This was (pleasantly) surprising because the diversity of genetic alterations in these three tumor cell types suggested that E1a might override many different genetic abnormalities to perhaps universally suppress (human) transformation. (aacrjournals.org)
EGFR/MEK/ERK signaling induces JUN/activator protein 1 activation, which is essential for oncogenic transformation, in combination with the GLI activator forms GLI1 and GLI2. (aacrjournals.org)
This, the third volume then looks at transformation and E1A, adenovirus genetics, pathogenesis and gene therapy. (springer.com)
E6-- E6 Transformation of host cell by binding p53 tumour suppressor protein. (kenyon.edu)
Cellular targets for transformation by the adenovirus E1A proteins. (wikipedia.org)

It is shown here that E1a has the two functions characteristic of a typical cellular activator: one direct E1a to the promoter, perhaps by interacting with a DNA-bound protein, and the other, an activating region, enables the bound activator to stimulate transcription. (nih.gov)
Cotransfection of a plasmid expressing carboxy-terminal amino acids 332 to 414 of YY1 fused to the GAL4 DNA-binding domain can inhibit expression from a reporter construct with GAL4 DNA binding sites in its promoter, and inclusion of a third plasmid expressing E1A proteins can relieve the repression. (princeton.edu)
Infection with an E1A - virus, followed 6 h later by superinfection with a wild-type virus, led to preferential transcriptional activation of the E1A gene of the first virus, suggesting that a host transcription component(s) stably associated with the E1A promoter in the absence of E1A protein and that this complex was the substrate for transcriptional activation by E1A protein. (princeton.edu)
The limiting host transcription component(s) bound to the E1A promoter to form a complex with a half-life greater than 24 h in the absence of E1A 289R protein, as demonstrated in a challenge assay with a large excess of superinfecting virus. (princeton.edu)
SAHA enhanced acetylation of histone H3 in Bim promoter region, while E1A upregulated Egr-1, which was directly involved in Bim transactivation. (nature.com)
Three vectors, all with mutated E1B55K, were created, one without any promoter controlling the E1a gene and two vectors with the E1a gene being controlled by either its endogenous promoter or a strong CMV promoter. (nih.gov)
As (i) HHV-8 and Ad may co-exist in immunocompromised patients and (ii) E1A might be considered as therapeutic transgene for HHV-8-associated neoplasms we investigated whether the promoter of the latency-associated nuclear antigen (LANAp) controlling expression of vCyclin, vFLIP, and LANA proteins required for latent type infection is regulated by E1A. (openvirologyjournal.com)
In particular, an Ad12 E1A mutant, Spm2, lacking six consecutive alanine residues in the "spacer" region activated the HHV-8 promoter about 15-fold compared to vector controls. (openvirologyjournal.com)
In summary, we report the activation of the LANAp by E1A as a novel interaction of E1A with a viral promoter. (openvirologyjournal.com)
In these adenoviruses, the E4 region (AdΔ24TyrE4) or both E1 and E4 regions (Ad2xTyr) were controlled by a synthetic tyrosinase enhancer/promoter (Tyr2E/P) specific for melanocytes. (aacrjournals.org)
For example, the yeast SAGA complex recruits TATA-binding proteins to the promoter, and also contains a ubiquitin hydrolase that facilitates the cycling of protein (de)ubiquitinylation [ 8-10 ]. (sigmaaldrich.com)
We hypothesized that A33 promoter might be useful in the design of a conditionally replicative adenovirus for the treatment of colorectal cancer (CRC). (aacrjournals.org)
To increase the efficacy and decrease the side-effect, we constructed a conditionally replicative adenovirus (CRAd) expressing human endostatin, with a human Telomoerase Reverse Transcriptase (hTERT) promoter for the regulation of the early stage of adenovirus expression of gene E1a and a Hypoxia Response Element (HRE) promoter to regulate the gene E1b. (lu.se)
Genomic organization is more complex in p63 and p73, largely the result of an alternative internal promoter generating NH 2 -terminally deleted dominant-negative proteins that engage in inhibitory circuits within the family. (aacrjournals.org)
The L4-33K protein is an alternative RNA splicing factor inducing L1-IIIa mRNA splicing, while L4-22K is stimulating transcription from the major late promoter (MLP). (diva-portal.org)

Interaction with RBX1 and CUL1 inhibits ubiquitination of the proteins targeted by SCF(FBXW7) ubiquitin ligase complex, and may be linked to unregulated host cell proliferation. (uniprot.org)
this interaction inhibits E1A mediated transactivation (PubMed:11733528). (rcsb.org)
It functions as a transcriptional repressor, and expression of E1A inhibits this repression. (abcam.com)
E1a inhibits the transactivation of this gene by p53, which tends to favor apoptosis versus growth arrest. (aacrjournals.org)
BS69, a novel adenovirus E1A-associated protein that inhibits E1A transactivation. (abnova.com)
Irvin, "Purification and Partial Characterization of the Antiviral Protein from Phytolacca americana Which Inhibits Eukaryotic Protein Synthesis," Archives of Biochemistry and Biophysics 169: 522-528, 1975. (patentgenius.com)

We therefore undertook an extensive site-directed mutagenesis approach to generate specific point mutants and to precisely map the binding sites for CBP, p300, TATA-binding protein (TBP), S4, S8, hGcn5, P/CAF, and Ran within the first 30 amino acids of the Ad5 12S E1A protein. (asm.org)
Interestingly, although TBP and S8 or CBP/p300 can exist as functional complexes, RNA interference revealed that the recruitment of either TBP, S8, or CBP/p300 to AdE1A was not dependent upon the expression of the other proteins. (asm.org)
The N-terminal region and elements in CR1 are required for binding the transcriptional coactivator proteins p300 and CBP ( 1 , 15 ). (asm.org)
These regions interact to form the binding sites for two classes of cellular proteins: those, such as the retinoblastoma gene product, whose association with the E1A products is specifically dependent on region 2, and another class which so far is known to include only a large cellular DNA-binding protein, p300, whose association with the E1A products is specifically dependent on the N-terminal region. (researchwithrutgers.com)
A combination of site-directed point mutagenesis and monoclonal antibody competition experiments reported here suggests that p300 binding is dependent on specific, conserved residues in the N terminus, including positively charged residues at positions 2 and 3 of the E1A proteins, and that p300 and pRB bind to distinct, nonoverlapping subregions within conserved region 1. (researchwithrutgers.com)
CBP (CREB-binding protein) and p300 are versatile coactivators that link transcriptional activators to the basal transcriptional apparatus. (pnas.org)
Direct physical interactions of CBP/p300 with p65 were demonstrated by glutathione S -transferase fusion protein binding, and coimmunoprecipitation/Western blot studies. (pnas.org)
The adenovirus E1A 12S protein, which complexes with CBP and p300, inhibited p65-dependent gene expression. (pnas.org)
Reporter gene expression could be rescued from E1A inhibition by overexpression of CBP or p300. (pnas.org)
A closely related cofactor, p300, was independently isolated on the basis of its interactions with adenovirus E1A ( 23 ). (pnas.org)
Regulation of histone acetyltransferases p300 and PCAF by the bHLH protein Twist and adenoviral oncoprotein E1A. (nature.com)
Phosphorylation of the adenovirus-associated p300 kDa protein in response to retinoic acid and E1A during the differentiation of F9 cells. (nature.com)
This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. (univ-mrs.fr)

These sequences are also required for these proteins to bind to the retinoblastoma gene product (pRb). (semanticscholar.org)
Association between an oncogene and an anti-oncogene: the adenovirus E1A proteins bind to the retinoblastoma gene product. (wikipedia.org)

These considerations have led to the development of conditionally replicative adenovirus (CRAds) for cancer therapy ( 5 , 6 ). (aacrjournals.org)
We next constructed a conditionally replicative adenovirus named AV22EL where E1A was placed under the control of A33Pr. (aacrjournals.org)

Adenovirus E1A proteins stimulate inositol phospholipid metabolism in PC12 cells. (asm.org)
To study the influence of nuclear oncogenes on inositol phospholipid metabolism, we examined the various parameters of inositol phospholipid metabolism in PC12 cells expressing adenovirus type 12 or adenovirus type 5 E1A. (asm.org)
Although the inositol 1,4,5-trisphosphate content was increased only slightly, the diacylglycerol content was 2.4-fold higher in E1A-expressing PC12 cells. (asm.org)
Overall protein kinase C activities in E1A-expressing PC12 cells were decreased, but the activity of membrane-bound protein kinase C was significantly increased. (asm.org)
These observations clearly indicate that inositol phospholipid metabolism is stimulated in cells producing E1A and suggest that nuclear oncogene E1A has the ability to stimulate inositol phospholipid metabolism. (asm.org)
The purified Escherichia coli-expressed products of the human subgroup-C adenovirus E1A 13 S and 12 S mRNAs are shown to induce cellular DNA synthesis when introduced by microinjection into quiescent, G 0-arrested mammalian cells from immortalized cell lines. (naver.com)
The E1A proteins stimulated cellular DNA synthesis in mouse Swiss 3T3 cells, when microinjected either individually or in combination. (naver.com)
A truncated EIA protein, in which 169 carboxyl terminal residues of the 289-amino acid E1A 13 S mRNA product are deleted, was unable to induce cellular DNA synthesis in these cells. (naver.com)
Here, we showed that proapoptotic activity of HDACi was robustly enhanced by E1A in multiple cancer cells, but not in normal cells. (nature.com)
SAHA downregulated Bcl-XL and upregulated proapoptotic BH3-only protein Bim, whose expression was further enhanced by E1A in cancer cells. (nature.com)
These alterations of Bcl-2 family proteins were critical for apoptosis induced by the combination in cancer cells. (nature.com)
In this study, we found that E1A efficiently enhanced cytotoxic effects of SAHA in a variety of human cancer cells. (nature.com)
To obtain a general idea of which anti-cancer drugs are most effectively potentiated by E1A gene therapy in vitro , we transiently transfected human cancer cells with either E1A expression or empty plasmid, and then treated the cells with various anti-cancer drugs, including 5-fluorouracil, cisplatin, etoposide, paclitaxel and SAHA and assessed apoptosis by measuring the caspase activity ( Supplementary Figure S1 ). (nature.com)
Future efforts in the development of transcription-controlled oncolytic adenoviruses should focus on how to completely block E1a expression in normal cells. (nih.gov)
FIELD OF THE INVENTION The invention relates to the field of recombinant protein production, in particular to the glycosylation of recombinant proteins such as erythropoietin, more in particular to glycosylation of recombinant proteins when produced in adenovirus ElA-expressing cells. (allindianpatents.com)
As shown in WO 00/63403, immortalized human embryonic retina cells expressing at least an adenovirus El A protein can be suitably used for the production of recombinant proteins. (allindianpatents.com)
Recombinant proteins having N-linked glycosylation produced in cells that express adenovirus El A have a specific glycosylation profile, for instance characterized by the presence of Lewis-X structures, as described in WO 03/038100. (allindianpatents.com)
Another characteristic of the proteins produced thus far in E1A expressing cells appeared a relatively low galactosylation and low sialylation of the N-linked glycans (WO 03/038100). (allindianpatents.com)
For other proteins similar situations may exist, i.e. for certain uses the specific glycosylation pattern observed upon expression in El A-expressing cells may be highly beneficial, while for other purposes a different glycosylation profile may be more suitable. (allindianpatents.com)
In particular, the interplay and potential competition between the various glycosyltransferases and other actors in the glycosylation process in cells that express El A, rendered the outcome of over-expression of a sialyltransferase in such cells unforeseen and unpredictable in terms of glycosylation patterns of proteins thus produced. (allindianpatents.com)
Transfection experiments in MV3 melanoma cells revealed activation of the LANAp by Ad5 E1A constructs containing an intact N terminus (aa 1-119). (openvirologyjournal.com)
On the other hand, E1A protein of nontumorigenic adenoviruses, such as for example Advertisement5, cannot suppress MHC-I appearance, Quetiapine manufacture leaving cells changed by Advertisement5 susceptible to reputation and eradication by web host CTLs (3, 7, 29, 39). (globaltechbiz.com)
Generally in most relaxing and unstimulated cells, NF-B is certainly maintained in the cytoplasm by IB proteins. (globaltechbiz.com)
The fact that the IE1 and IE2 gene products are able to inhibit apoptosis raised the interesting possibility that they might cooperate with the adenovirus E1A oncoproteins to transform primary rodent cells, as do the adenovirus E1B 19-kDa (E1B) protein and the cellular Bcl-2 protein, each of which can block apoptosis ( 15 , 16 ). (pnas.org)
In this report, we show that the IE1 and IE2 gene products can, indeed, cooperate with E1A to transform primary baby rat kidney (BRK) cells. (pnas.org)
We have generated novel conditionally replicative adenoviruses (CRAds) targeted to melanoma cells. (aacrjournals.org)
The properties of these CRAds were compared with wild-type adenovirus (Adwt) and our previous CRAd with a targeted E1A CRII mutation (AdTyrΔ24) in submerged cultures of melanoma cells and nonmelanoma control cells. (aacrjournals.org)
Fourth and importantly, in productive infections, host cells are lysed and release their progeny, but the viruses are usually eliminated in a few days by antibodies to the capsid proteins. (aacrjournals.org)
In 1991, two groups published tumor-suppressive effects of E1a in human tumor cells. (aacrjournals.org)
Since 2004 scientists have found that brain tumors are driven by haywire stem cells that replicate themselves, differentiate into other types of cells, and bear protein markers like normal stem cells. (eurekalert.org)
All four lines exhibited the characteristics and protein signatures of stem cells. (eurekalert.org)
The nuclear localization signal of NF-κB is masked by the binding of an inhibitory protein, IκB, sequestering NF-κB in the cytoplasm in unstimulated cells. (pnas.org)
The antiapoptotic protein cellular FLICE (Fas-associated death domain-like IL-1β-converting enzyme) inhibitory protein (cFLIP) protects cells from CD95(APO-1/Fas)-induced apoptosis in vitro and was found to be overexpressed in human melanomas. (rupress.org)
In the reactivation phase, the expression dynamics of KSHV immediate-early or early lytic proteins such as RTA, K9 (vIRF1), K5, K3, ORF59, and K8 (K-bZIP) were comparable between wild-type, control BC3, and BC3-PML KO cells. (frontiersin.org)
Adenoviruses attacking normal cells employ their own protein, E1A, to counteract retinoblastoma's defensive measures. (medindia.net)
To keep Delta-24-RGD out of normal cells, Fueyo and colleagues deleted a small part of the gene that produces E1A. (medindia.net)
In this thesis we have investigated the functional significance of the pVII protein and VA RNAI in HAdV-5 infected cells. (diva-portal.org)
Our studies show that the cellular E3 ubiquitin ligase MKRN1 is a novel pVII interacting protein in HAdV-5 infected cells. (diva-portal.org)
MKRN1 expression reduced the pVII protein accumulation in virus-infected cells and affected infectious virus formation. (diva-portal.org)
Our results revealed that the Vaccinia virus E3L protein was able to partially substitute for the HAdV-5 VA RNAI functions in virus-infected cells. (diva-portal.org)
The protein kinase C activator, phorbol ester, elicits disparate functional responses in androgen-sensitive and androgen-independent human prostatic cancer cells. (biomedsearch.com)
The protein kinase C (PKC) activator 12-O-tetradecanoyl-phorbol-13-acetate (TPA) activated cell death in androgen-sensitive LNCaP cells but not in androgen-independent DU-145 or PC-3 cells, whose growth was significantly decreased by PKC inhibitors staurosporine and H7. (biomedsearch.com)
53 (1), 167-176 (2018) PUBMED 29658564 REMARK GeneRIF: the present study indicated that CtBP2 reduced the susceptibility of ECA109 cells to cisplatin by regulating the expression of apoptosis-related proteins, suggesting that it may be a promising therapeutic target in esophageal squamous cell carcinoma in the future. (genome.jp)
Our previous studies have shown that BFT rapidly (by 1 min) cleaves E-cadherin, an intercellular adhesion protein forming the zonula adherens of intestinal epithelial cells, and that cleavage of E-cadherin correlates with the onset of morphologic changes in the cells (occurring by 10 min after BFT treatment of HT29/C1 cells) ( 47 ). (asm.org)
AV22EL exhibited no cytocidal effect on normal human colonic cells and hepatocytes, whereas these cells were eliminated by wild-type adenovirus. (aacrjournals.org)
I. To determine the maximal tolerated dose (MTD) of allogeneic bone marrow-derived human mesenchymal stem cells (BM-hMSCs) loaded with the oncolytic adenovirus DNX-2401 (BM-hMSCs-DNX2401) administered by intra-arterial injection (i.e., transfemoral super-selective endovascular intracranial injection) in patients with recurrent glioblastoma (GBM), gliosarcoma or wild-type IDH-1 anaplastic astrocytoma. (clinicaltrials.gov)
Oncogenes of DNA tumor viruses encode proteins that cause cells to divide incessantly, eventually leading to formation of a tumor. (virology.ws)
The genomes of adenoviruses, polyomaviruses, and papillomaviruses encode proteins that cause cells to divide. (virology.ws)
These viral proteins kick cells into mitosis by inactivating cell proteins (such as Rb, pictured) that are normally involved in regulating cell growth. (virology.ws)
Cells transformed with T, E6/E7, or E1A proteins are commonly used in laboratories because they are immortal. (virology.ws)
Another commonly used transformed cell line is 293 (human embryonic kidney cells transformed by adenovirus E1A). (virology.ws)
HEK 293T/17 cells were transformed with adenovirus E1a carrying a temperature sensitive T antigen co-selected with neomycin. (atcc.org)
In fact, cells have dedicated significant resources to developing ways of recognizing histone tail modifications as evidenced by the recent identification of multiple protein modalities dedicated to recognizing various histone methylation marks. (childrenshospital.org)
We found an immune reaction of PML and Sp100 proteins with a PIC1/SUMO-1-specific monoclonal antibody by immunoblotting when using cell extracts prepared from stably transfected cells inducibly expressing one isoform of each protein as well as from nontransfected cells. (rupress.org)
The endostatin expression in a cultured supernatant from tumor cells was significantly higher than that obtained from non-duplicative adenovirus vectors carrying that gene. (lu.se)
[7] [8] Should an oncogenic protein, such as those produced by cells infected by high-risk types of human papillomaviruses , bind and inactivate pRb, this can lead to cancer. (wikipedia.org)
However, a serious concern exists for using HER2-targeting gene therapy with E1A, as E1A is a potent viral oncoprotein that can transform primary cells. (aacrjournals.org)
Microinjection of the ras oncogene protein into PC12 cells induces morphological differentiation. (cshlpress.com)
Gel mobility shift assays indicated that nuclear extracts purified from cells grown under low-CO 2 conditions in light contained DNA-binding proteins specifically interacting with EE-1 and EE-2. (plantphysiol.org)
These EEC-binding proteins were present even in cells grown under high-CO 2 conditions (5% [v/v]) or in the dark when Cah1 is not activated. (plantphysiol.org)
15 , 16 However, EBV also enhances antiviral immune responses by way of its latent membrane protein 1 (LMP1), which up-regulates MHC-I. 17 , 18 This leads to cytotoxic T-cell (CTL)-mediated elimination of EBV latency III cells, promoting the transition to latency I-infected B cells. (bloodjournal.org)
However, late gene expression, expression of structural proteins and vegetative DNA synthesis is restricted to terminally differentiated cells of the epidermis which implies a link between cellular differentiation and viral gene expression. (kenyon.edu)
Baatz JE, Bruno MD, Ciraolo PJ, Glasser SW, Stripp BR, Smyth KL, Korfhagen TR (1994) Utilization of modified surfactant-associated protein B for delivery of DNA to airway cells in culture. (springer.com)
Bai M, Harfe B, Freimuth P (1993) Mutations that alter an Arg-Gly-Asp (RGD) sequence in the adenovirus type 2 penton base protein abolish its cell rounding activity and delay virus reproduction in flat cells. (springer.com)
Chen P, Omelles D, Shenk T (1993) The adenovirus L3 23-kilodalton proteinase cleaves the armino-terminal head domain from cytokeratin 18 and disrupts the cytokeratin network of HeLa cells. (springer.com)
Cotten M, Wagner E, Zatloukal K, Bimstiel ML (1993b) Chicken adenovirus (CELO virus) particles augment receptor-mediated DNA delivery to mammalian cells and yield exceptional levels of stable transformants. (springer.com)
Cotten M, Baker A, Saltik M, Wagner E, Buschle M (1994a) Lipopolysaccharide is a frequent contaminant of plasmid DNA preparations and can be toxic to primary cells in the presence of adenovirus. (springer.com)
Gelonin, a New Inhibitor of Protein Synthesis, Nontoxic to Intact Cells," Journal of Biological Chemistry 255(14): 6947-6953, 1980. (patentgenius.com)
Several cellular proteins form stable complexes with the proteins encoded by the adenovirus early region 1A (E1A) gene in extracts derived from adenovirus infected or transformed cells. (sciencemag.org)
STATs (signal transducers and activators of transcription) are a family of latent cytoplasmic proteins that are activated to participate in gene control when cells encounter various extracellular polypeptides. (sciencemag.org)

Transcription activation by the adenovirus E1a protein. (nih.gov)
Adenovirus E1A, simian virus 40 tumor antigen, and human papillomavirus E7 protein share the capacity to disrupt the interaction between transcription factor E2F and the retinoblastoma gene product. (semanticscholar.org)
E1A 138 and 12S mRNA products made in Escherichia coli both function as nucleus-localized transcription activators but do not directly bind DNA. (naver.com)
The adenovirus 125 and 135 E1A proteins have been shown to relieve repression mediated by the cellular transcription factor YY1. (princeton.edu)
The 135 E1A protein not only relieves repression but also activates transcription through YY1 binding sites. (princeton.edu)
We propose that E1A proteins normally relieve YY1-mediated transcriptional repression by binding directly to the cellular transcription factor. (princeton.edu)
Synthesis of the E1A 289R protein by the first virus to infect reduced inhibition of transcription of the superinfecting virus, indicating that the E1A 289R protein was limiting for E1A-activated transcription. (princeton.edu)
The kinetics of the activation suggest that this was due to an indirect effect rather than to destabilization of stable transcription complexes by the 289R protein. (princeton.edu)
Adenoviral (Ad) E1A proteins regulate the activity of cellular and viral promoters/enhancers and transcription factors and can suppress tumorigenicity of human cancers. (openvirologyjournal.com)
These results demonstrate that two independent domains of p53 can potentially interact with the TATA-binding protein, and they define a mechanism-relief of repression-by which the 13S E1A oncoprotein can activate transcription through the TATA motif. (northwestern.edu)
This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. (rcsb.org)
This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. (genecards.org)
A second class of proteins important in the initiation of transcription by RNA polymerase II are the coactivators (i.e., proteins that bridge the transcriptional activators and the components of the basal transcriptional apparatus). (pnas.org)
We have used GAL4-directed transcription to expand the domain of embryonic expression of the homeobox protein even-skipped. (biologists.org)
Jun dimerization protein-2 (JDP2) is a component of the AP-1 transcription factor that represses transactivation mediated by the Jun family of proteins. (nature.com)
These results suggest that the sequence-specific DNA-binding protein JDP2 may control transcription via direct regulation of the modification of histones and the assembly of chromatin. (nature.com)
The GLI zinc finger transcription factors act at the end of the HH signaling cascade to control gene expression, and recent studies have shown that the activity of GLI proteins can be additionally modified by integration of distinct signals, such as the MEK/extracellular signal-regulated kinase (ERK) and phosphinositide-3 kinase (PI3K)/AKT pathway. (aacrjournals.org)
Its regulation involves a variety of signalling proteins and small molecules, and occurs at multiple levels, including transcription, translation and post-translation levels. (portlandpress.com)
These proteins, collectively known as pocket proteins, inhibit cell proliferation by sequestration of a number of growth-promoting proteins, the most prominent of which is a cellular transcription factor named E2F. (searlescholars.net)
Selective interaction of JNK protein kinase isoforms with transcription factors. (nii.ac.jp)
Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. (genetex.com)
Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. (genetex.com)
TATA binding protein (TBP) and transcription factor IIB (TFIIB) are key factors for the assembly of eukaryotic transcription initiation complexes. (plantcell.org)
In other eukaryotes, the in vitro assembly of the transcription complex is initiated by the interaction between the TATA binding protein (TBP), which is the central component of transcription factor IIB (TFIIB), and the TATA box. (plantcell.org)
RB/E2F-family proteins repress transcription. (wikipedia.org)
We show that MHC-I transcription is regulated by the KSHV-encoded viral FLICE inhibitory protein (vFLIP) and by viral IFN regulatory factor 1 (vIRF1). (bloodjournal.org)
This thesis discusses adenoviral regulation of transcription and splicing by two proteins expressed at the late phase: L4-22K and L4-33K. (diva-portal.org)
Although L4-22K is reported to be an MLP transcriptional enhancer protein, here we show that L4-22K also functions as a repressor of MLP transcription. (diva-portal.org)
Biochemical and molecular genetic explorations have defined a single tyrosine phosphorylation site and, in a dimeric partner molecule, an Src homology 2 (SH2) phosphotyrosine-binding domain, a DNA interaction domain, and a number of protein-protein interaction domains (with receptors, other transcription factors, the transcription machinery, and perhaps a tyrosine phosphatase). (sciencemag.org)

Indeed, the mutation of L at positions 19 and 20 eliminated the ability of AdE1A to interact with any of the N-terminal binding proteins studied here. (asm.org)
Two domains on the E1A proteins interact with YY1: an amino-terminal sequence (residues 15 to 35) that is present in both E1A proteins and a domain that includes at least a portion of conserved region 3 (residues 140 to 188) that is present in the 135 but not the 125 E1A protein. (princeton.edu)
Two domains on YY1 interact with E1A proteins: one is contained within residues 54 to 260, and the other is contained within the carboxy-terminal domain of YY1 (residues 332 to 414). (princeton.edu)
Thus, we find a correlation between the ability of E1A to interact with the carboxy-terminal domain of YY1 and its ability to relieve repression caused by the carboxy-terminal domain of YY1. (princeton.edu)
Since histone proteins are highly basic and can interact extensively with the negatively charged DNA, acetylation may weaken histone-DNA interactions, hence modulating the structure of the underlying locus. (sigmaaldrich.com)
Collectively, these proteins interact with components of the basal transcriptional apparatus to effect dramatic changes in gene expression (for review, see ref. 1 ). (pnas.org)
Activators that stimulate DA-complex assembly also interact directly with TFIIA, as observed by coimmunoprecipitation and affinity column chromatography with recombinant proteins ( 24 , 32 ). (asm.org)
To study cell cycle regulation by pocket proteins, we have isolated cDNAs for cellular proteins that interact with p107 and 130. (searlescholars.net)
We have cloned two novel cellular proteins that interact with E1A, named BS69 and mUBC9. (searlescholars.net)
In mammals, extracellular ligands, such as peptide hormones or neurotransmitters, interact with transmembrane proteins called G-coupled receptors (GPCR). (moleculardevices.com)
The Myb proteins interact with a highly conserved multi-protein complex called the MuvB core. (stanford.edu)
Both proteins can also interact with a polycomb group protein complex which participates in regulation of gene expression during development. (thermofisher.com)
In this paper, we describe a conserved DNA sequence in the two enhancer elements involved in the transcriptional activation of Cah1 under low-CO 2 conditions and the presence of DNA-binding proteins that interact with the enhancer elements in nuclear extracts. (plantphysiol.org)

These data further indicate that AdE1A can target individual partner proteins in vivo and that it does not necessarily recruit these proteins indirectly as components of larger macromolecular complexes. (asm.org)
May also participate in the formation of NuA4 related complexes which lack the KAT5 /TIP60 catalytic subunit, but which include the SWI/SNF related protein SRCAP . (rcsb.org)
This DA-complex assembly activity can be conveniently assayed in vitro by an electrophoretic mobility shift assay (EMSA) using agarose gels to separate the large DNA-protein complexes involved ( 28 ). (asm.org)
1998 ). The promyelocytic leukaemia gene product (PML) forms stable complexes with the retinoblastoma protein. (biologists.org)
Proteins that enter the nucleus in preformed complexes may not require their own targeting motif ( Dingwall,1982 ). (eu.org)
1994). In this review I will provide a summary of the current methods of generating adenovirus-DNA transfection complexes as well as discussing two of the toxicity problems that we have encountered with this system and describing some solutions to these problems. (springer.com)

Regulation of adenoviral gene expression is a complex process directed by viral proteins controlling a multitude of different activities at distinct phases of the virus life cycle. (diva-portal.org)

A biochemical investigation of the adenovirus-induced G1 to S progression: Thymidine kinase, ornithine decarboxylase, and inhibitors of polyamine biosynthesis. (naver.com)
It's been proposed the fact that phosphorylation of p65 Ser276 with the proteins kinase A catalytic subunit (PKAc) promotes transactivation activity by launching the C-terminal transactivation area from an intramolecular masking with the N-terminal area (44). (globaltechbiz.com)
Elk-1 is associated with a dimer of serum response factor (SRF) at the SRE site, and its phosphorylation occurs at specific residues in response to mitogen-activated protein kinases (MAPKs), including c-Jun-N terminal kinase (JNK), p38/MAPK, and extracellular-signal regulated kinase (ERK). (frontiersin.org)
1991 ) Signalling by the sevenless protein tyrosine kinase is mimicked by Ras1 activation. (biologists.org)
Repression of GCN5 histone acetyltransferase activity via bromodomain-mediated binding and phosphorylation by the Ku-DNA-dependent protein kinase complex. (nature.com)
Our data suggest that BFT-stimulated IL-8 secretion involves tyrosine kinase-dependent activation of nuclear factor-κB (NF-κB) as well as activation of the mitogen-activated protein kinases (MAPKs), p38 and extracellular signal-related kinase. (asm.org)
However, it took a long time to realise that Cdc2 and cyclins form a stoichiometric complex and that a cyclin subunit is necessary for the Cdc2 subunit to gain its protein kinase activity. (biologists.org)
cdc2 protein kinase is complexed with both cyclin A and B: Evidence for proteolytic inactivation of MPF. (cshlpress.com)
Deregulation of the TSC/mTOR signaling pathway has been implicated in the development of cancer and the activation of ribosomal protein S6 kinase (S6K1) by mTOR was found to lead to increased cell proliferation and angiogenesis. (ncl.edu.tw)
the kinase activity was modulated during the cell division cycle, and association of pRb with E1A apparently was not required for this activity. (sciencemag.org)

The amino- and carboxy-terminal p53 interactions occur within the domain on the TATA-binding protein to which the adenovirus 13S E1A oncoprotein has previously been shown to bind. (northwestern.edu)
The 13S E1A oncoprotein can dissociate the complex formed between the carboxy-terminal domain of p53 and the TATA-binding protein and relieve p53-mediated transcriptional repression. (northwestern.edu)
Histone acetyltransferase activity of CBP is controlled by cycle-dependent kinases and oncoprotein E1A. (nature.com)

5. The adenovirus according to claim 1, wherein its genome lacks the E1 region. (freepatentsonline.com)
6. The adenovirus according to claim 5, comprising a region of another animal or human adenovirus genome. (freepatentsonline.com)
Only one strand of the genome is transcribed, producing two classes of proteins: the non-structural regulatory proteins, known as early proteins, and the structural proteins L1 and L2, known as late proteins. (kenyon.edu)

Several KSHV proteins regulate host innate or adaptive immune responses. (bloodjournal.org)
Cold-induced RNA-binding proteins regulate circadian gene expression by controlling alternative polyadenylation. (sibcb.ac.cn)

The LXCXE domain (residues 103 to 107) mediates binding to the retinoblastoma family proteins pRB, p107, and p130 ( 5 , 6 , 9 , 19 , 51 ). (asm.org)
Recent studies have shown that E7 promotes degradation of Rb family proteins rather than simply inhibiting their function by complex formation. (novusbio.com)
Thus, p53 family proteins take on functions within a wide biological spectrum stretching from development (p63 and p73), DNA damage response via apoptosis and cell cycle arrest (p53, TAp63, and TAp73), chemosensitivity of tumors (p53 and TAp73), and immortalization and oncogenesis (ΔNp73). (aacrjournals.org)

Opposing oncogenic activities of small DNA tumor virus transforming proteins. (biomedsearch.com)
Promyelocytic leukemia protein (PML), a component of nuclear domain 10 (ND10), PML oncogenic domain (POD) or PML nuclear bodies (PML-NB), has tumor suppressive and antiviral defense activities. (frontiersin.org)

Conditionally replicative oncolytic adenoviruses (CRAd) have shown promising applications in cancer gene therapy ( 7 , 8 ). (aacrjournals.org)

This gene encodes TBP, the TATA-binding protein. (genecards.org)
It encodes a homolog of the animal C-terminal binding proteins (CtBPs). (uni-koeln.de)
HAdV encodes the pVII protein, which is involved in nuclear delivery, protection and expression of viral DNA. (diva-portal.org)

High-mobility group protein 1 (HMGB1) is a nonhistone nuclear protein whose function depends on cellular location. (jimmunol.org)
High-mobility group protein 1 (HMGB1) 3 is a nonhistone nuclear protein that has both intracellular and extracellular functions. (jimmunol.org)
Shown in A) are five nuclear proteins ( LIN-1 , SUR-2 , LIN-25 , EOR-1 , EOR-2 ) that are jointly important for vulval, excretory duct and P12 cell fates. (wormbook.org)
1 (1967) 891), of DNA and nuclear proteins (Kaneda et al. (freepatentsonline.com)
PML and Sp100 proteins are associated with nuclear domains, known as nuclear dots (NDs). (rupress.org)
This demonstrated that protein synthesis is required at each cell cycle for chromosome condensation and nuclear envelope breakdown and thus for the G2/M phase transition to occur. (biologists.org)
1995 ). Targeting of adenovirus E1a and E4-orf3 proteins to nuclear matrix associated PML bodies. (biologists.org)
1999 ). SUMO-1 modification of the acute promyelocytic leukaemia protein PML: implications for nuclear organisation. (biologists.org)
Many nuclear proteins possess a nuclear localisation signal (NLS) that is recognised by the importer protein importin-alpha. (eu.org)
The latter is docked on the cytosolic side of the nuclear pore via repetitive FG, FxFG and GLFG linear motifs found in several nucleoporin proteins (FG-Nups) ( Terry,2009 ). (eu.org)
Proteins which function in the nucleus pass through the nuclear envelope via the nuclear pores. (eu.org)
Therefore, almost all well studied nuclear proteins are transported into the nucleus using active translocation through the pore. (eu.org)
A striking feature of protein transfer through the nuclear pore is that key roles are played by several linear motifs. (eu.org)

More especially, the present invention relates to the use of recombinant adenoviruses of animal origin as vectors for gene therapy. (freepatentsonline.com)
All the vectors derived from the adenoviruses described in the prior art for the purposes of use in gene therapy in humans have hitherto been prepared from adenoviruses of human origin. (freepatentsonline.com)

Previous work has demonstrated an in vitro interaction between p53 and the TATA-binding protein that requires amino acids 20 to 57 of p53 and amino acids 220 to 271 of the TATA-binding protein. (northwestern.edu)
The present results show that a 75-amino-acid segment from the carboxy terminus of p53 also can bind to the TATA-binding protein in vitro, and this interaction requires amino acids 217 to 268 of the TATA-binding protein, essentially the same domain that is required for interaction with the amino-terminal domain of p53. (northwestern.edu)
the interaction enhances ATF7 -mediated viral transactivation activity which requires the zinc binding domains of both proteins (By similarity). (rcsb.org)
this interaction stimulates the acetylation of RB1 by recruiting EP300 and RB1 into a multimeric-protein complex (PubMed:11433299). (rcsb.org)
this interaction allows E1A import into the host nucleus (PubMed:23864635). (rcsb.org)
others may involve the interaction of E1a with the retinoblastoma protein (Rb), whereas yet others depend on the interaction of E1a with a transcriptional corepressor known as COOH-terminal binding protein (CtBP). (aacrjournals.org)
Second, acetylation may create a new protein-protein interaction interface. (sigmaaldrich.com)
Interaction studies identified several RNA binding proteins that are associated with AN. (uni-koeln.de)
We provide evidence that the NAD(H) binding domain of AN is relevant in this context as proteins carrying mutations in this domain localize to a much higher degree to SGs and strongly reduce AN dimerization and its interaction with one interactor but not the others. (uni-koeln.de)
Considering that HER2 is a heat shock protein 90 (Hsp90) client protein and requires interaction with Hsp90 and its chaperone to acquire proper protein function ( 9 ), the Hsp90 inhibitor such as geldanamycin provides an alternative approach to target HER2 through dissociation of HER2 from the chaperone, leading to HER2 degradation by a proteasome-dependent manner ( 10 ). (aacrjournals.org)
Taken with previous findings on the effects of infection by herpes simplex virus and adenovirus on ND10 structure and PML modification, these results suggest that the many factors which have been shown to modify ND10 structure may do so by interaction with the biochemical mechanisms that act on ND10 components during the cell cycle. (biologists.org)
Lower panel: Protein interaction domains in the STATs listed at the left. (sciencemag.org)

Rb is a multifunctional protein with many binding and phosphorylation sites. (wikipedia.org)
Within each subunit, there are a variety of protein binding sites, as well as a total of 15 possible phosphorylation sites. (wikipedia.org)
Generally, phosphorylation causes interdomain locking, which changes Rb's conformation and prevents binding to target proteins. (wikipedia.org)
Phosphorylation on a single tyrosine located around residue 700 in each protein is obligatory for STAT activation ( 1 , 2 ) (Fig. 1 ). (sciencemag.org)

one, a 105-kilodalton protein (pRb), is the product of the retinoblastoma gene, and the other, a 60-kilodalton protein, is a human cyclin A. Two other proteins that bind E1A have now been shown to be related to p34cdc2. (sciencemag.org)

19 Among these there are 5 viral proteins that block the innate antiviral interferon (IFN) response, including orf45, 20 viral IL-6 (vIL6), 21 viral interferon regulatory factors (vIRFs) 1 and 2, 22 ⇓ ⇓ ⇓ ⇓ - 27 and the transactivator of the lytic cycle, RTA. (bloodjournal.org)

Alterations to controls of cellular DNA synthesis by adenovirus infection. (naver.com)
Surprisingly, the endogenous MKRN1 protein underwent proteasomal degradation during the prolonged HAdV-5 infection. (diva-portal.org)
Oncolytic adenovirus DNX-2401 is made from the common cold virus that has been changed in the laboratory to make it less likely to cause an infection (such as a cold). (clinicaltrials.gov)
In case of HIV-1 infection, it is recruited by the viral protein Tat. (proteopedia.org)

Our results provide evidence that E1A proteins can function, independent of other viral functions, in the stimulation of cellular DNA synthesis in certain cell types. (naver.com)
They achieve this by a number of mechanisms, including direct lysis, apoptosis, expression of toxic proteins, autophagy and shut-down of protein synthesis, as well as the induction of anti-tumoral immunity. (mdpi.com)
At this time, their cytoplasm has gained MPF activity and, even in the absence of protein synthesis ( Wasserman and Masui, 1975 ), it drives recipient oocytes into M phase when transferred into G2-arrested oocytes. (biologists.org)
The same year, Wagenaar analyzed the timing of synthesis of proteins required for mitosis in the early cell cycles of sea urchin embryos ( Wagenaar, 1983 ), extending previous reports that puromycin added at the time of fertilization prevents the first division ( Hultin, 1961 ). (biologists.org)
11 ⇓ - 13 EBV modulates cellular antiviral responses in various ways, including down-regulation of major histocompatibility complex (MHC) proteins 14 and blocking proteasomal degradation and antigen synthesis. (bloodjournal.org)

We determined that although common residues within the N-terminal region can form partial binding sites for these proteins, point mutants were also generated that could discriminate between binding sites. (asm.org)
While region 2 has been studied intensively, very little is known so far concerning the nature of the essential residues in the N-terminal region, or about the manner in which conserved region 1 participates in the binding of two distinct sets of cellular proteins. (researchwithrutgers.com)
REFERENCE 3 (residues 1 to 445) AUTHORS Bellesis AG, Jecrois AM, Hayes JA, Schiffer CA and Royer WE Jr. TITLE Assembly of human C-terminal binding protein (CtBP) into tetramers JOURNAL J. Biol. (genome.jp)
The CR2 homology region contains the LXCXE motif (residues 22-26) involved in binding to the tumor suppressor protein pRb. (novusbio.com)

The tumorigenesis-restraining activity of E1A may be related to the disruption of the host CtBP-CtIP complex through the CtBP binding motif. (uniprot.org)
A 48 kDa cellular protein doublet (designated CtBP) was found to bind specifically to the GST-E1a C-terminal fusion protein as well as to bacterially expressed full-length E1a (243R) protein. (nih.gov)

Adenovirus-induced alterations of the cell growth cycle: a requirement for expression of E1A but not of E1B. (naver.com)
The other report (6) , which was from the Hung laboratory, indicated that E1a suppressed tumor growth by repressing HER2/c-erbB2/c-neu expression. (aacrjournals.org)
We have directed expression of an activated form of the Dras2 protein, resulting in dominant eye and wing defects that can be used in screens to identify other members of the Dras2 signal transduction pathway. (biologists.org)
1988 ) Expression, function, and regulation of the hairy segmentation protein in the Drosophila embryo. (biologists.org)
In addition, we show that inhibition of the Cul3 protein reduces HAdV-5 E1A gene expression. (diva-portal.org)
Its systemic administration induced E1A expression only in the hepatic metastasis but not in normal organs. (aacrjournals.org)
90% of mice and restored normal levels of hepatic enzymes following metastases disappearance, exhibiting no evidence of viral activity in normal organs, including normal intestine, as assessed by E1A and luciferase expression. (aacrjournals.org)
1. A recombinant canine CAV-2 adenovirus, comprising at least one inserted human gene and sequences permitting expression of the inserted gene in a human cell in which the recombinant adenovirus is infectious. (freepatentsonline.com)
Both proteins are expressed in the form of many COOH-terminally spliced variants, and their expression is enhanced by interferons (IFN). (rupress.org)
The viral protein such as adenovirus E1A has been reported to suppress tumor growth through repressing HER2 expression ( 8 ). (aacrjournals.org)
Association of Programmed Death 1 Protein Ligand (PD-L1) Expression With Prognosis in Merkel Cell Carcinoma. (dana-farber.org)
Bridge E, Ketner G (1989) Redundant control of adenovirus late gene expression by early region 4. (springer.com)
My future research will combine the approaches of protein biochemistry, molecular biology, and genomics/proteomics to further analyze the functional relationship of Mediator to other molecular and cellular events, such as the coupling of the signal transduction and gene expression, chromosomal modification and remodeling, cell growth and differentiation, and cancer development. (sibcb.ac.cn)

FBPs are structurally and functionally diverse, and their activity is crucial for selecting proteins that will be targeted by the SCF complex. (mdpi.com)
In papers III and IV, we tested the ability of various plant and animal virus encoded RNAi/miRNA and IFN suppressor proteins to functionally substitute for the HAdV-5 VA RNAI. (diva-portal.org)
They are structurally and functionally similar to the E1A protein of subgenus C adenoviruses. (novusbio.com)
Thus, the sequence-based conservation is mirrored by the functions, as expected for functionally important proteins. (diva-portal.org)

Moreover, we showed here, the underlying molecular mechanisms of HDACi-induced apoptosis enhanced by E1A. (nature.com)
We have previously reported ( 13 ) that the two most abundantly expressed immediate-early gene products of HCMV, IE1 and IE2 (reviewed in ref. 14 ), inhibit the induction of apoptosis by tumor necrosis factor α (TNF-α) or the adenovirus E1A oncoproteins. (pnas.org)
E1a causes p53 protein to accumulate (17) , and p53 can induce either growth arrest or apoptosis, depending on the cellular context. (aacrjournals.org)

This increase in TP53, in turn, would arrest the cell proliferation and direct its death but this effect is counteracted by the viral protein E1B-55K. (uniprot.org)
Investigations using E1A proteins have illuminated important paradigms in cell proliferation and about the functions of cellular proteins such as the retinoblastoma protein. (biomedsearch.com)
Association of the E7 protein with pRb promotes cell proliferation by the same mechanism as the E1A proteins of adenoviruses and SV40 large T antigen. (novusbio.com)

Adenovirus 5 early region 1A (E1A) has been shown to exhibit high tumor suppressor activity, and gene therapy using E1A has been tested in clinical trials. (nature.com)
Moreover, we showed that combination of E1A gene therapy and SAHA showed high therapeutic efficacy with low toxicity in vivo ovarian and breast xenograft models. (nature.com)
combined paclitaxel and E1A gene therapy is currently being investigated in a clinical trial for ovarian cancer patients. (nature.com)
Combined treatment using E1A gene therapy and SAHA also showed highly effective anti-tumor activity in ovarian and breast cancer xenograft models, with lesser toxicity than that of E1A and paclitaxel. (nature.com)
These results indicate that the combination therapy of E1A gene therapy and SAHA would be an attractive therapeutic strategy for treating human cancers. (nature.com)
A Multicenter Phase I Gene Therapy Clinical Trial Involving Intraperitoneal Administration of E1A-Lipid Complex in Patients with Recurrent Epithelial Ovarian Cancer Overexpressing HER-2/ neu Oncogene. (aacrjournals.org)
Among these viruses, the adenoviruses display certain properties which are advantageous for use in gene therapy. (freepatentsonline.com)

BS69 is a factor previously identified as an adenovirus E1A-associated protein. (abcam.com)

The F-box domain is a protein structural motif of about 50 amino acids that mediates protein-protein interactions. (mdpi.com)
This review describes the role of F-box proteins and the use of the ubiquitin-proteasome system in virus-host interactions. (mdpi.com)
Besides the F-box domain, other domains and motifs related to protein-protein interactions are usually present in the C -terminal region of FBPs, such as leucine rich repeats (LRR), WD40 repeats (WD), Kelch repeats, proline-rich and others. (mdpi.com)
these interactions inhibit RBX1 - CUL1 -dependent elongation reaction of ubiquitin chains and attenuate ubiquitination of SCF( FBXW7 ) target proteins (PubMed:19679664). (rcsb.org)
Transcriptional regulation, a critical control mechanism in fundamental biologic processes, requires the participation of several classes of proteins: those that bind specific DNA sequences, those that associate with transcriptional regulators through protein-protein interactions, known as transcriptional coactivators or corepressors, and those that perform an architectural function. (pnas.org)
Moreover, COOH-terminal extensions specific for p63 and p73 enable further unique protein-protein interactions with regulatory pathways involved in development, differentiation, proliferation, and damage response. (aacrjournals.org)

No significant correlation between in vivo activation function and in vitro binding to human TATA binding protein, human TFIIB, or Drosophila melanogaster TAF II 40 was observed for this set of VP16C mutants. (asm.org)
For these reasons, adenoviruses have already been used for in vivo gene transfer. (freepatentsonline.com)
Adenovirus-Mediated Transfer of a Rcombinant .alpha.1-Antitrypsin Gene to the Lung Epithelium in Vivo," Science 252: 431-434, 1991. (patentgenius.com)
L4-22K lacks the ability to enhance L1-IIIa splicing in vivo , and here we show that the protein is defective in L1-IIIa or other late pre-mRNA splicing reactions in vitro . (diva-portal.org)

In this study, we identified a new chimera gene between the transactivation domain of EWS and E1A-F, encoding the adenovirus E1A enhancer-binding protein. (nih.gov)
The p65 subunit, similar to two others in the κB family, RelB and c-Rel, contains two transactivation domains in the C-terminal region of the protein ( 8 ). (pnas.org)
We have shown previously that the retinoblastoma protein-related p107 can form a specific complex with the MYC transactivation domain, causing inhibition of MYC transactivation. (searlescholars.net)

The adenovirus E1A gene product, the simian virus 40 large tumor antigen, and the human papillomavirus E7 protein share a short amino acid sequence that constitutes a domain required for the transforming activity of these proteins. (semanticscholar.org)
A33 antigen is a membrane-bound protein expressed in intestinal epithelium that is overexpressed in 95% of primary and metastatic colorectal carcinomas but is absent in most epithelial tissues and tumor types. (aacrjournals.org)

3. The adenovirus according to claim 1, further comprising sequences encoding a signal sequence enabling secretion of an encoded protein of the inserted human gene. (freepatentsonline.com)
However, the detailed nucleotide sequences of cis-acting elements and presence of DNA-binding proteins related to Cah1 regulation have not been identified. (plantphysiol.org)
Belin M.-T, Boulanger P (1993) Involvement of cellular adhesion sequences in the attachment of adenovirus to the HeLa cell surface. (springer.com)

The HPV E7 proteins are small (HPV16 E7 comprising 98 amino acids, 15kDa), zinc binding phosphoproteins which are localized in the nucleus. (novusbio.com)
The first 16 amino-terminal amino acids of HPV16 E7 contain a region homologous to a segment of the conserved region 1 (CR1) of the E1A protein of subgenus C adenoviruses. (novusbio.com)

One protein is a transcriptional repressor, while the other isoform is a major component of specialized synapses known as synaptic ribbons. (genome.jp)

First, like cellular DnaJ proteins, SV40 TAg can bind specifically to a member of the Hsp70 family of heat shock proteins ( 36 ). (asm.org)

First, wild-type adenoviruses (Adwt) are nononcogenic in humans. (aacrjournals.org)
For example, adenoviruses do not cause cancer in humans but are instead responsible for colds, conjunctivitis and other acute illnesses. (wikipedia.org)
In humans, the protein is encoded by the RB1 gene located on chromosome 13-more specifically, 13q14.1-q14.2 . (wikipedia.org)

Over-expressing of a sialyltransferase in a cell to increase sialylation of recombinant proteins produced in that cell has been described for other cell types (e.g. (allindianpatents.com)
The present invention resides in use of a recombinant adenovirus of animal origin containing a heterologous DNA sequence for the preparation of a pharmaceutical composition intended for the therapeutic and/or surgical treatment of the human body. (freepatentsonline.com)

This essay focuses on one well-studied epigenetic control, histone (de)acetylation, and the recent development of cancer therapy aiming at altering the balance of histone and protein acetylation. (sigmaaldrich.com)
Control of the histone-acetyltransferase activity of Tip60 by the HIV-1 transactivator protein, Tat. (nature.com)

Human being adenovirus type 12 (Advertisement12) E1A proteins (E1A-12) may be the crucial determinant of viral tumorigenesis. (globaltechbiz.com)
Individual adenovirus type 12 (Advertisement12) is certainly capable of leading to tumors Quetiapine manufacture when released into immunocompetent adult rodents (4, 25, 26, 38). (globaltechbiz.com)

Association between the E1A products and either class of cellular proteins can be disrupted by mutations in conserved region 1. (researchwithrutgers.com)
Physical mapping of temperature-sensitive mutations of adenoviruses. (cshlpress.com)

Delta-24-RGD exploits the fact that a protein called retinoblastoma (Rb) is either missing or defective in brain tumors. (eurekalert.org)

This fusion protein was used to purify cellular proteins that bind to the C-terminal region of E1a. (nih.gov)

Human adenoviruses (HAdVs) contain linear, double-stranded DNA with an average genomic length of 35 kbp. (cdc.gov)
So far, characterizations of the L4-22K and L4-33K proteins have been limited to human adenoviruses 2 or 5 (HAdV-2/5). (diva-portal.org)

Mouse genetics experiments have defined crucial roles for each known mammalian STAT. The discovery of a STAT in Drosophila , and most recently in Dictyostelium discoideum , implies an ancient evolutionary origin for this dual-function set of proteins. (sciencemag.org)

In the presence of the E1A 289R protein, the E1A gene of the superinfecting virus was gradually activated with a reduction in E1A mRNA accumulation from the first virus. (princeton.edu)
Interestingly, the E3L protein rescued the translational defect but did not stimulate viral capsid mRNA accumulation observed with VA RNA. (diva-portal.org)
Both proteins selectively upregulated E1A-10S mRNA accumulation in transfection experiments, by a mechanism independent of the tiny RS-repeat. (diva-portal.org)

The EE-1- and EE-2-binding proteins interacted with the EECs contained in both of the two enhancer elements in vitro. (plantphysiol.org)
On the basis of these results, the relationship between the transcriptional regulation of Cah1 and protein-binding to the enhancer elements in the 5′-upstream region of Cah1 is discussed. (plantphysiol.org)
The L4-33K protein contains a tiny RS-repeat in its unique C-terminal end that is essential for the splicing enhancer function of the protein. (diva-portal.org)

Sam-D b is a mouse embryo fibroblast transfected with a plasmid encoding the E1A epitope coupled to a signal sequence and murine H-2D b and therefore presents high levels of the Ad5E1A epitope in the context of H-2D b . (rupress.org)

Anatomy16

Organisms29

Diseases11

Chemicals and Drugs89

Analytical, Diagnostic and Therapeutic Techniques and Equipment25

Phenomena and Processes67

Information Science3

B-MYB transactivates its own promoter through SP1-binding sites. (1/910)

A viral mechanism for inhibition of p300 and PCAF acetyltransferase activity. (2/910)

Regulation of histone acetyltransferases p300 and PCAF by the bHLH protein twist and adenoviral oncoprotein E1A. (3/910)

Recruitment of the retinoblastoma protein to c-Jun enhances transcription activity mediated through the AP-1 binding site. (4/910)

Mutant p53 can provoke apoptosis in p53-deficient Hep3B cells with delayed kinetics relative to wild-type p53. (5/910)

Differences in the interactions of oncogenic adenovirus 12 early region 1A and nononcogenic adenovirus 2 early region 1A with the cellular coactivators p300 and CBP. (6/910)

Transcriptional regulation of the mouse ferritin H gene. Involvement of p300/CBP adaptor proteins in FER-1 enhancer activity. (7/910)

The adenoviral E1A oncoproteins interfere with the growth-inhibiting effect of the cdk-inhibitor p21(CIP1/WAF1). (8/910)

Adenovirus E1A Proteins

Adenoviruses, Human

Adenovirus E4 Proteins

Adenovirus E1B Proteins

Adenovirus Early Proteins

Adenovirus E3 Proteins

Adenoviridae

Adenovirus Infections, Human

Adenovirus E2 Proteins

Adenoviridae Infections

Adenovirus E1 Proteins

Oncogene Proteins, Viral

Genetic Vectors

HeLa Cells

Cell Line

Mastadenovirus

Adenoviruses, Canine

Molecular Sequence Data

Transcription, Genetic

Genes, Viral

DNA, Viral

Cell Transformation, Viral

E1A-Associated p300 Protein

Promoter Regions, Genetic

Transcription Factors

Base Sequence

DNA-Binding Proteins

Transfection

Gene Expression Regulation, Viral

Retinoblastoma Protein

Antigens, Viral, Tumor

Virus Replication

Transcription Factor DP1

Adenoviruses, Porcine

Viral Proteins

Aviadenovirus

Retinoblastoma-Binding Protein 1

Genetic Therapy

Nuclear Proteins

E2F Transcription Factors

Fowl adenovirus A

Gene Transfer Techniques

Transcriptional Activation

Amino Acid Sequence

Mutation

Capsid Proteins

Plasmids

Gene Expression Regulation

Trans-Activators

Recombinant Fusion Proteins

Protein Binding

Tumor Cells, Cultured

Simian virus 40

Binding Sites

Cell Line, Transformed

Receptors, Virus

Tumor Suppressor Protein p53

Oncolytic Virotherapy

Activating Transcription Factors

RNA, Messenger

Antigens, Polyomavirus Transforming

Oncolytic Viruses

Keratoconjunctivitis

Transduction, Genetic

Cell Nucleus

Retinoblastoma-Like Protein p107

CREB-Binding Protein

Cell Transformation, Neoplastic

Chloramphenicol O-Acetyltransferase

Cells, Cultured

Apoptosis

DNA Replication

Papillomavirus E7 Proteins

Gene Expression

E2F1 Transcription Factor

Repressor Proteins

RNA, Viral

Oncogenes

KB Cells

Conjunctivitis, Viral