Proteins transcribed from the E1A genome region of ADENOVIRUSES which are involved in positive regulation of transcription of the early genes of host infection.
Species of the genus MASTADENOVIRUS, causing a wide range of diseases in humans. Infections are mostly asymptomatic, but can be associated with diseases of the respiratory, ocular, and gastrointestinal systems. Serotypes (named with Arabic numbers) have been grouped into species designated Human adenovirus A-F.
Proteins transcribed from the E4 region of ADENOVIRUSES. The E4 19K protein transactivates transcription of the adenovirus E2F protein and complexes with it.
Proteins transcribed from the E1B region of ADENOVIRUSES which are involved in regulation of the levels of early and late viral gene expression.
Proteins encoded by adenoviruses that are synthesized prior to, and in the absence of, viral DNA replication. The proteins are involved in both positive and negative regulation of expression in viral and cellular genes, and also affect the stability of viral mRNA. Some are also involved in oncogenic transformation.
Proteins transcribed from the E3 region of ADENOVIRUSES but not essential for viral replication. The E3 19K protein mediates adenovirus persistence by reducing the expression of class I major histocompatibility complex antigens on the surface of infected cells.
A family of non-enveloped viruses infecting mammals (MASTADENOVIRUS) and birds (AVIADENOVIRUS) or both (ATADENOVIRUS). Infections may be asymptomatic or result in a variety of diseases.
Respiratory and conjunctival infections caused by 33 identified serotypes of human adenoviruses.
Proteins transcribed from the E2 region of ADENOVIRUSES. Several of these are required for viral DNA replication.
Virus diseases caused by the ADENOVIRIDAE.
The very first viral gene products synthesized after cells are infected with adenovirus. The E1 region of the genome has been divided into two major transcriptional units, E1A and E1B, each expressing proteins of the same name (ADENOVIRUS E1A PROTEINS and ADENOVIRUS E1B PROTEINS).
Products of viral oncogenes, most commonly retroviral oncogenes. They usually have transforming and often protein kinase activities.
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
Established cell cultures that have the potential to propagate indefinitely.
A genus of ADENOVIRIDAE that infects MAMMALS including humans and causes a wide range of diseases. The type species is Human adenovirus C (see ADENOVIRUSES, HUMAN).
Species of the genus MASTADENOVIRUS that causes fever, edema, vomiting, and diarrhea in dogs and encephalitis in foxes. Epizootics have also been caused in bears, wolves, coyotes, and skunks. The official species name is Canine adenovirus and it contains two serotypes.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
The functional hereditary units of VIRUSES.
Deoxyribonucleic acid that makes up the genetic material of viruses.
An inheritable change in cells manifested by changes in cell division and growth and alterations in cell surface properties. It is induced by infection with a transforming virus.
A member of the p300-CBP transcription factors that was originally identified as a binding partner for ADENOVIRUS E1A PROTEINS.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Any of the processes by which cytoplasmic factors influence the differential control of gene action in viruses.
Product of the retinoblastoma tumor suppressor gene. It is a nuclear phosphoprotein hypothesized to normally act as an inhibitor of cell proliferation. Rb protein is absent in retinoblastoma cell lines. It also has been shown to form complexes with the adenovirus E1A protein, the SV40 T antigen, and the human papilloma virus E7 protein.
Those proteins recognized by antibodies from serum of animals bearing tumors induced by viruses; these proteins are presumably coded for by the nucleic acids of the same viruses that caused the neoplastic transformation.
The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.
A transcription factor that possesses DNA-binding and E2F-binding domains but lacks a transcriptional activation domain. It is a binding partner for E2F TRANSCRIPTION FACTORS and enhances the DNA binding and transactivation function of the DP-E2F complex.
Species of the genus MASTADENOVIRUS, causing neurological disease in pigs.
Proteins found in any species of virus.
A genus of ADENOVIRIDAE that infects birds. The type species is FOWL ADENOVIRUS A.
A ubiquitously expressed regulatory protein that contains a retinoblastoma protein binding domain and an AT-rich interactive domain. The protein may play a role in recruiting HISTONE DEACETYLASES to the site of RETINOBLASTOMA PROTEIN-containing transcriptional repressor complexes.
Techniques and strategies which include the use of coding sequences and other conventional or radical means to transform or modify cells for the purpose of treating or reversing disease conditions.
Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.
A family of basic helix-loop-helix transcription factors that control expression of a variety of GENES involved in CELL CYCLE regulation. E2F transcription factors typically form heterodimeric complexes with TRANSCRIPTION FACTOR DP1 or transcription factor DP2, and they have N-terminal DNA binding and dimerization domains. E2F transcription factors can act as mediators of transcriptional repression or transcriptional activation.
The type species of the genus AVIADENOVIRUS, family ADENOVIRIDAE, an oncogenic virus of birds. This is also called CELO virus for chick embryo lethal orphan virus.
The introduction of functional (usually cloned) GENES into cells. A variety of techniques and naturally occurring processes are used for the gene transfer such as cell hybridization, LIPOSOMES or microcell-mediated gene transfer, ELECTROPORATION, chromosome-mediated gene transfer, TRANSFECTION, and GENETIC TRANSDUCTION. Gene transfer may result in genetically transformed cells and individual organisms.
Processes that stimulate the GENETIC TRANSCRIPTION of a gene or set of genes.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Proteins that form the CAPSID of VIRUSES.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
A species of POLYOMAVIRUS originally isolated from Rhesus monkey kidney tissue. It produces malignancy in human and newborn hamster kidney cell cultures.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Eukaryotic cell line obtained in a quiescent or stationary phase which undergoes conversion to a state of unregulated growth in culture, resembling an in vitro tumor. It occurs spontaneously or through interaction with viruses, oncogenes, radiation, or drugs/chemicals.
Specific molecular components of the cell capable of recognizing and interacting with a virus, and which, after binding it, are capable of generating some signal that initiates the chain of events leading to the biological response.
Nuclear phosphoprotein encoded by the p53 gene (GENES, P53) whose normal function is to control CELL PROLIFERATION and APOPTOSIS. A mutant or absent p53 protein has been found in LEUKEMIA; OSTEOSARCOMA; LUNG CANCER; and COLORECTAL CANCER.
Use of attenuated VIRUSES as ANTINEOPLASTIC AGENTS to selectively kill CANCER cells.
Activating transcription factors were originally identified as DNA-BINDING PROTEINS that interact with early promoters from ADENOVIRUSES. They are a family of basic leucine zipper transcription factors that bind to the consensus site TGACGTCA of the cyclic AMP response element, and are closely related to CYCLIC AMP-RESPONSIVE DNA-BINDING PROTEIN.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Polyomavirus antigens which cause infection and cellular transformation. The large T antigen is necessary for the initiation of viral DNA synthesis, repression of transcription of the early region and is responsible in conjunction with the middle T antigen for the transformation of primary cells. Small T antigen is necessary for the completion of the productive infection cycle.
Tumor-selective, replication competent VIRUSES that have antineoplastic effects. This is achieved by producing cytotoxicity-enhancing proteins and/or eliciting an antitumor immune response. They are genetically engineered so that they can replicate in CANCER cells but not in normal cells, and are used in ONCOLYTIC VIROTHERAPY.
Simultaneous inflammation of the cornea and conjunctiva.
The transfer of bacterial DNA by phages from an infected bacterium to another bacterium. This also refers to the transfer of genes into eukaryotic cells by viruses. This naturally occurring process is routinely employed as a GENE TRANSFER TECHNIQUE.
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
A negative regulator of the CELL CYCLE that undergoes PHOSPHORYLATION by CYCLIN-DEPENDENT KINASES. It contains a conserved pocket region that binds E2F4 TRANSCRIPTION FACTOR and interacts with viral ONCOPROTEINS such as POLYOMAVIRUS TUMOR ANTIGENS; ADENOVIRUS E1A PROTEINS; and PAPILLOMAVIRUS E7 PROTEINS.
A member of the p300-CBP transcription factor family that was initially identified as a binding partner for CAMP RESPONSE ELEMENT-BINDING PROTEIN. Mutations in CREB-binding protein are associated with RUBINSTEIN-TAYBI SYNDROME.
Cell changes manifested by escape from control mechanisms, increased growth potential, alterations in the cell surface, karyotypic abnormalities, morphological and biochemical deviations from the norm, and other attributes conferring the ability to invade, metastasize, and kill.
An enzyme that catalyzes the acetylation of chloramphenicol to yield chloramphenicol 3-acetate. Since chloramphenicol 3-acetate does not bind to bacterial ribosomes and is not an inhibitor of peptidyltransferase, the enzyme is responsible for the naturally occurring chloramphenicol resistance in bacteria. The enzyme, for which variants are known, is found in both gram-negative and gram-positive bacteria. EC 2.3.1.28.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
The process by which a DNA molecule is duplicated.
ONCOGENE PROTEINS from papillomavirus that deregulate the CELL CYCLE of infected cells and lead to NEOPLASTIC CELL TRANSFORMATION. Papillomavirus E7 proteins have been shown to interact with various regulators of the cell cycle including RETINOBLASTOMA PROTEIN and certain cyclin-dependent kinase inhibitors.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
An E2F transcription factor that interacts directly with RETINOBLASTOMA PROTEIN and CYCLIN A and activates GENETIC TRANSCRIPTION required for CELL CYCLE entry and DNA synthesis. E2F1 is involved in DNA REPAIR and APOPTOSIS.
Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release.
Ribonucleic acid that makes up the genetic material of viruses.
Genes whose gain-of-function alterations lead to NEOPLASTIC CELL TRANSFORMATION. They include, for example, genes for activators or stimulators of CELL PROLIFERATION such as growth factors, growth factor receptors, protein kinases, signal transducers, nuclear phosphoproteins, and transcription factors. A prefix of "v-" before oncogene symbols indicates oncogenes captured and transmitted by RETROVIRUSES; the prefix "c-" before the gene symbol of an oncogene indicates it is the cellular homolog (PROTO-ONCOGENES) of a v-oncogene.
This line KB is now known to be a subline of the ubiquitous KERATIN-forming tumor cell line HeLa. It was originally thought to be derived from an epidermal carcinoma of the mouth, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via contamination by HELA CELLS. The cells are positive for keratin by immunoperoxidase staining. KB cells have been reported to contain human papillomavirus18 (HPV-18) sequences.
Inflammation, often mild, of the conjunctiva caused by a variety of viral agents. Conjunctival involvement may be part of a systemic infection.
Proteins that control the CELL DIVISION CYCLE. This family of proteins includes a wide variety of classes, including CYCLIN-DEPENDENT KINASES, mitogen-activated kinases, CYCLINS, and PHOSPHOPROTEIN PHOSPHATASES as well as their putative substrates such as chromatin-associated proteins, CYTOSKELETAL PROTEINS, and TRANSCRIPTION FACTORS.
Cis-acting DNA sequences which can increase transcription of genes. Enhancers can usually function in either orientation and at various distances from a promoter.
Transport proteins that carry specific substances in the blood or across cell membranes.
Vaccines used to prevent infection by any virus from the family ADENOVIRIDAE.
A conserved A-T rich sequence which is contained in promoters for RNA polymerase II. The segment is seven base pairs long and the nucleotides most commonly found are TATAAAA.
A sequence of successive nucleotide triplets that are read as CODONS specifying AMINO ACIDS and begin with an INITIATOR CODON and end with a stop codon (CODON, TERMINATOR).
A genus of the family PARVOVIRIDAE, subfamily PARVOVIRINAE, which are dependent on a coinfection with helper adenoviruses or herpesviruses for their efficient replication. The type species is Adeno-associated virus 2.
The outer protein protective shell of a virus, which protects the viral nucleic acid.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Proteins prepared by recombinant DNA technology.
Serologic tests in which a positive reaction manifested by visible CHEMICAL PRECIPITATION occurs when a soluble ANTIGEN reacts with its precipitins, i.e., ANTIBODIES that can form a precipitate.
The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.
A phosphoprotein phosphatase subtype that is comprised of a catalytic subunit and two different regulatory subunits. At least two genes encode isoforms of the protein phosphatase catalytic subunit, while several isoforms of regulatory subunits exist due to the presence of multiple genes and the alternative splicing of their mRNAs. Protein phosphatase 2 acts on a broad variety of cellular proteins and may play a role as a regulator of intracellular signaling processes.
Enzymes catalyzing the transfer of an acetyl group, usually from acetyl coenzyme A, to another compound. EC 2.3.1.
Genes that are introduced into an organism using GENE TRANSFER TECHNIQUES.
A general transcription factor that plays a major role in the activation of eukaryotic genes transcribed by RNA POLYMERASES. It binds specifically to the TATA BOX promoter element, which lies close to the position of transcription initiation in RNA transcribed by RNA POLYMERASE II. Although considered a principal component of TRANSCRIPTION FACTOR TFIID it also takes part in general transcription factor complexes involved in RNA POLYMERASE I and RNA POLYMERASE III transcription.
Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected.
The major sequence-specific DNA-binding component involved in the activation of transcription of RNA POLYMERASE II. It was originally described as a complex of TATA-BOX BINDING PROTEIN and TATA-BINDING PROTEIN ASSOCIATED FACTORS. It is now know that TATA BOX BINDING PROTEIN-LIKE PROTEINS may take the place of TATA-box binding protein in the complex.
Visible morphologic changes in cells infected with viruses. It includes shutdown of cellular RNA and protein synthesis, cell fusion, release of lysosomal enzymes, changes in cell membrane permeability, diffuse changes in intracellular structures, presence of viral inclusion bodies, and chromosomal aberrations. It excludes malignant transformation, which is CELL TRANSFORMATION, VIRAL. Viral cytopathogenic effects provide a valuable method for identifying and classifying the infecting viruses.
Products of proto-oncogenes. Normally they do not have oncogenic or transforming properties, but are involved in the regulation or differentiation of cell growth. They often have protein kinase activity.
A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.
Viruses which enable defective viruses to replicate or to form a protein coat by complementing the missing gene function of the defective (satellite) virus. Helper and satellite may be of the same or different genus.
A cell line derived from cultured tumor cells.
Tumor suppressor genes located on human chromosome 13 in the region 13q14 and coding for a family of phosphoproteins with molecular weights ranging from 104 kDa to 115 kDa. One copy of the wild-type Rb gene is necessary for normal retinal development. Loss or inactivation of both alleles at this locus results in retinoblastoma.
Head to tail array of covalently joined DNA sequences generated by concatenation. Concatenated DNA is attached end to end in contrast to CATENATED DNA which is attached loop to loop.
Viruses which lack a complete genome so that they cannot completely replicate or cannot form a protein coat. Some are host-dependent defectives, meaning they can replicate only in cell systems which provide the particular genetic function which they lack. Others, called SATELLITE VIRUSES, are able to replicate only when their genetic defect is complemented by a helper virus.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
Mutant mice homozygous for the recessive gene "nude" which fail to develop a thymus. They are useful in tumor studies and studies on immune responses.
DNA viruses producing malignant tumors. Of the six major groupings of DNA viruses four contain members which are actually or potentially oncogenic: the Adenoviridae, the Herpesviridae, the Papovaviridae, and the Poxviridae.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
A sequence of amino acids in a polypeptide or of nucleotides in DNA or RNA that is similar across multiple species. A known set of conserved sequences is represented by a CONSENSUS SEQUENCE. AMINO ACID MOTIFS are often composed of conserved sequences.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
The sum of the weight of all the atoms in a molecule.
A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.
A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.
Genes whose expression is easily detectable and therefore used to study promoter activity at many positions in a target genome. In recombinant DNA technology, these genes may be attached to a promoter region of interest.
Biochemical identification of mutational changes in a nucleotide sequence.
Actual loss of portion of a chromosome.
Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). Lines have been developed using several different strains of mice. Tissues are usually fibroblasts derived from mouse embryos but other types and sources have been developed as well. The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION.
A negative regulator of the CELL CYCLE that undergoes PHOSPHORYLATION by CYCLIN-DEPENDENT KINASES. RBL2 contains a conserved pocket region that binds E2F4 TRANSCRIPTION FACTOR and E2F5 TRANSCRIPTION FACTOR. RBL2 also interacts with viral ONCOPROTEINS such as POLYOMAVIRUS TUMOR ANTIGENS; ADENOVIRUS E1A PROTEINS; and PAPILLOMAVIRUS E7 PROTEINS.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
A genus of the family PICORNAVIRIDAE whose members preferentially inhabit the intestinal tract of a variety of hosts. The genus contains many species. Newly described members of human enteroviruses are assigned continuous numbers with the species designated "human enterovirus".
Structures that are part of or contained in the CELL NUCLEUS.
A family of histone acetyltransferases that is structurally-related to CREB-BINDING PROTEIN and to E1A-ASSOCIATED P300 PROTEIN. They function as transcriptional coactivators by bridging between DNA-binding TRANSCRIPTION FACTORS and the basal transcription machinery. They also modify transcription factors and CHROMATIN through ACETYLATION.
A subclass of enzymes which includes all dehydrogenases acting on primary and secondary alcohols as well as hemiacetals. They are further classified according to the acceptor which can be NAD+ or NADP+ (subclass 1.1.1), cytochrome (1.1.2), oxygen (1.1.3), quinone (1.1.5), or another acceptor (1.1.99).
An E2F transcription factor that interacts directly with RETINOBLASTOMA PROTEIN and CYCLIN A. E2F2 activates GENETIC TRANSCRIPTION required for CELL CYCLE entry and DNA synthesis.
A large family of regulatory proteins that function as accessory subunits to a variety of CYCLIN-DEPENDENT KINASES. They generally function as ENZYME ACTIVATORS that drive the CELL CYCLE through transitions between phases. A subset of cyclins may also function as transcriptional regulators.
Sequences of DNA or RNA that occur in multiple copies. There are several types: INTERSPERSED REPETITIVE SEQUENCES are copies of transposable elements (DNA TRANSPOSABLE ELEMENTS or RETROELEMENTS) dispersed throughout the genome. TERMINAL REPEAT SEQUENCES flank both ends of another sequence, for example, the long terminal repeats (LTRs) on RETROVIRUSES. Variations may be direct repeats, those occurring in the same direction, or inverted repeats, those opposite to each other in direction. TANDEM REPEAT SEQUENCES are copies which lie adjacent to each other, direct or inverted (INVERTED REPEAT SEQUENCES).
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.
Cellular DNA-binding proteins encoded by the c-jun genes (GENES, JUN). They are involved in growth-related transcriptional control. There appear to be three distinct functions: dimerization (with c-fos), DNA-binding, and transcriptional activation. Oncogenic transformation can take place by constitutive expression of c-jun.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
A genus of ADENOVIRIDAE that comprises viruses of several species of MAMMALS and BIRDS. The type species is Ovine adenovirus D.
Synthetic or natural oligonucleotides used in hybridization studies in order to identify and study specific nucleic acid fragments, e.g., DNA segments near or within a specific gene locus or gene. The probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the probe include the radioisotope labels 32P and 125I and the chemical label biotin.
Enzymes that oxidize certain LUMINESCENT AGENTS to emit light (PHYSICAL LUMINESCENCE). The luciferases from different organisms have evolved differently so have different structures and substrates.
A ubiquitously expressed zinc finger-containing protein that acts both as a repressor and activator of transcription. It interacts with key regulatory proteins such as TATA-BINDING PROTEIN; TFIIB; and ADENOVIRUS E1A PROTEINS.
Process of growing viruses in live animals, plants, or cultured cells.
A family of small, non-enveloped DNA viruses infecting birds and most mammals, especially humans. They are grouped into multiple genera, but the viruses are highly host-species specific and tissue-restricted. They are commonly divided into hundreds of papillomavirus "types", each with specific gene function and gene control regions, despite sequence homology. Human papillomaviruses are found in the genera ALPHAPAPILLOMAVIRUS; BETAPAPILLOMAVIRUS; GAMMAPAPILLOMAVIRUS; and MUPAPILLOMAVIRUS.
Tumor suppressor genes located on the short arm of human chromosome 17 and coding for the phosphoprotein p53.
A DNA-dependent RNA polymerase present in bacterial, plant, and animal cells. It functions in the nucleoplasmic structure where it transcribes DNA into RNA. It has specific requirements for cations and salt and has shown an intermediate sensitivity to alpha-amanitin in comparison to RNA polymerase I and II. EC 2.7.7.6.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
Proteins that are normally involved in holding cellular growth in check. Deficiencies or abnormalities in these proteins may lead to unregulated cell growth and tumor development.
Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.
The complete genetic complement contained in a DNA or RNA molecule in a virus.
A species of CERCOPITHECUS containing three subspecies: C. tantalus, C. pygerythrus, and C. sabeus. They are found in the forests and savannah of Africa. The African green monkey (C. pygerythrus) is the natural host of SIMIAN IMMUNODEFICIENCY VIRUS and is used in AIDS research.
A genus of potentially oncogenic viruses of the family POLYOMAVIRIDAE. These viruses are normally present in their natural hosts as latent infections. The virus is oncogenic in hosts different from the species of origin.
Cellular DNA-binding proteins encoded by the c-myc genes. They are normally involved in nucleic acid metabolism and in mediating the cellular response to growth factors. Elevated and deregulated (constitutive) expression of c-myc proteins can cause tumorigenesis.
The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990)
Enzymes that catalyze acyl group transfer from ACETYL-CoA to HISTONES forming CoA and acetyl-histones.
Nuclear antigen with a role in DNA synthesis, DNA repair, and cell cycle progression. PCNA is required for the coordinated synthesis of both leading and lagging strands at the replication fork during DNA replication. PCNA expression correlates with the proliferation activity of several malignant and non-malignant cell types.
The rate dynamics in chemical or physical systems.
The ultimate exclusion of nonsense sequences or intervening sequences (introns) before the final RNA transcript is sent to the cytoplasm.
A diverse class of enzymes that interact with UBIQUITIN-CONJUGATING ENZYMES and ubiquitination-specific protein substrates. Each member of this enzyme group has its own distinct specificity for a substrate and ubiquitin-conjugating enzyme. Ubiquitin-protein ligases exist as both monomeric proteins multiprotein complexes.
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
A family of transcription factors found primarily in PLANTS that bind to the G-box DNA sequence CACGTG or to a consensus sequence CANNTG.
Invasion of the host RESPIRATORY SYSTEM by microorganisms, usually leading to pathological processes or diseases.
Production of new arrangements of DNA by various mechanisms such as assortment and segregation, CROSSING OVER; GENE CONVERSION; GENETIC TRANSFORMATION; GENETIC CONJUGATION; GENETIC TRANSDUCTION; or mixed infection of viruses.
A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.
Immunoglobulins produced in response to VIRAL ANTIGENS.
Proteins coded by oncogenes. They include proteins resulting from the fusion of an oncogene and another gene (ONCOGENE PROTEINS, FUSION).
A protein that has been shown to function as a calcium-regulated transcription factor as well as a substrate for depolarization-activated CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASES. This protein functions to integrate both calcium and cAMP signals.
Minute infectious agents whose genomes are composed of DNA or RNA, but not both. They are characterized by a lack of independent metabolism and the inability to replicate outside living host cells.
Process of determining and distinguishing species of bacteria or viruses based on antigens they share.
An area showing altered staining behavior in the nucleus or cytoplasm of a virus-infected cell. Some inclusion bodies represent "virus factories" in which viral nucleic acid or protein is being synthesized; others are merely artifacts of fixation and staining. One example, Negri bodies, are found in the cytoplasm or processes of nerve cells in animals that have died from rabies.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Process of generating a genetic MUTATION. It may occur spontaneously or be induced by MUTAGENS.
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
Inflammation of the lung parenchyma that is caused by a viral infection.
A suborder of PRIMATES consisting of six families: CEBIDAE (some New World monkeys), ATELIDAE (some New World monkeys), CERCOPITHECIDAE (Old World monkeys), HYLOBATIDAE (gibbons and siamangs), CALLITRICHINAE (marmosets and tamarins), and HOMINIDAE (humans and great apes).
Elements of limited time intervals, contributing to particular results or situations.
Substances elaborated by viruses that have antigenic activity.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
A DNA-dependent RNA polymerase present in bacterial, plant, and animal cells. It functions in the nucleoplasmic structure and transcribes DNA into RNA. It has different requirements for cations and salt than RNA polymerase I and is strongly inhibited by alpha-amanitin. EC 2.7.7.6.
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
Membrane proteins encoded by the BCL-2 GENES and serving as potent inhibitors of cell death by APOPTOSIS. The proteins are found on mitochondrial, microsomal, and NUCLEAR MEMBRANE sites within many cell types. Overexpression of bcl-2 proteins, due to a translocation of the gene, is associated with follicular lymphoma.
A ubiquitously expressed complement receptor that binds COMPLEMENT C3B and COMPLEMENT C4B and serves as a cofactor for their inactivation. CD46 also interacts with a wide variety of pathogens and mediates immune response.
Deletion of sequences of nucleic acids from the genetic material of an individual.
Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.
A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.
The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.
Suspensions of attenuated or killed viruses administered for the prevention or treatment of infectious viral disease.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Motifs in DNA- and RNA-binding proteins whose amino acids are folded into a single structural unit around a zinc atom. In the classic zinc finger, one zinc atom is bound to two cysteines and two histidines. In between the cysteines and histidines are 12 residues which form a DNA binding fingertip. By variations in the composition of the sequences in the fingertip and the number and spacing of tandem repeats of the motif, zinc fingers can form a large number of different sequence specific binding sites.
Nucleic acid sequences involved in regulating the expression of genes.
CELL LINES derived from the CV-1 cell line by transformation with a replication origin defective mutant of SV40 VIRUS, which codes for wild type large T antigen (ANTIGENS, POLYOMAVIRUS TRANSFORMING). They are used for transfection and cloning. (The CV-1 cell line was derived from the kidney of an adult male African green monkey (CERCOPITHECUS AETHIOPS).)
INFLAMMATION of any segment of the GASTROINTESTINAL TRACT from ESOPHAGUS to RECTUM. Causes of gastroenteritis are many including genetic, infection, HYPERSENSITIVITY, drug effects, and CANCER.
The interaction of two or more substrates or ligands with the same binding site. The displacement of one by the other is used in quantitative and selective affinity measurements.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
Viruses that produce tumors.
Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.
The directional growth of an organism in response to an external stimulus such as light, touch, or gravity. Growth towards the stimulus is a positive tropism; growth away from the stimulus is a negative tropism. (From Concise Dictionary of Biology, 1990)
A genus of the family HERPESVIRIDAE, subfamily BETAHERPESVIRINAE, infecting the salivary glands, liver, spleen, lungs, eyes, and other organs, in which they produce characteristically enlarged cells with intranuclear inclusions. Infection with Cytomegalovirus is also seen as an opportunistic infection in AIDS.
Injections introduced directly into localized lesions.

B-MYB transactivates its own promoter through SP1-binding sites. (1/910)

B-MYB is an ubiquitous protein required for mammalian cell growth. In this report we show that B-MYB transactivates its own promoter through a 120 bp segment proximal to the transcription start site. The B-MYB-responsive element does not contain myb-binding sites and gel-shift analysis shows that SP1, but not B-MYB, protein contained in SAOS2 cell extracts binds to the 120 bp B-myb promoter fragment. B-MYB-dependent transactivation is cooperatively increased in the presence of SP1, but not SP3 overexpression. When the SP1 elements of the B-myb promoter are transferred in front of a heterologous promoter, an increased response to B-MYB results. In contrast, c-MYB, the prototype member of the Myb family, is not able to activate the luciferase construct containing the SP1 elements. With the use of an SP1-GAL4 fusion protein, we have determined that the cooperative activation occurs through the domain A of SP1. These observations suggest that B-MYB functions as a coactivator of SP1, and that diverse combinations of myb and SP1 sites may dictate the responsiveness of myb-target genes to the various members of the myb family.  (+info)

A viral mechanism for inhibition of p300 and PCAF acetyltransferase activity. (2/910)

Nucleosomal histone modification is believed to be a critical step in the activation of RNA polymerase II-dependent transcription. p300/CBP and PCAF histone acetyltransferases (HATs) are coactivators for several transcription factors, including nuclear hormone receptors, p53, and Stat1alpha, and participate in transcription by forming an activation complex and by promoting histone acetylation. The adenoviral E1A oncoprotein represses transcriptional signaling by binding to p300/CBP and displacing PCAF and p/CIP proteins from the complex. Here, we show that E1A directly represses the HAT activity of both p300/CBP and PCAF in vitro and p300-dependent transcription in vivo. Additionally, E1A inhibits nucleosomal histone modifications by the PCAF complex and blocks p53 acetylation. These results demonstrate the modulation of HAT activity as a novel mechanism of transcriptional regulation.  (+info)

Regulation of histone acetyltransferases p300 and PCAF by the bHLH protein twist and adenoviral oncoprotein E1A. (3/910)

Histone acetyltransferases (HAT) play a critical role in transcriptional control by relieving repressive effects of chromatin, and yet how HATs themselves are regulated remains largely unknown. Here, it is shown that Twist directly binds two independent HAT domains of acetyltransferases, p300 and p300/CBP-associated factor (PCAF), and directly regulates their HAT activities. The N terminus of Twist is a primary domain interacting with both acetyltransferases, and the same domain is required for inhibition of p300-dependent transcription by Twist. Adenovirus E1A protein mimics the effects of Twist by inhibiting the HAT activities of p300 and PCAF. These findings establish a cogent argument for considering the HAT domains as a direct target for acetyltransferase regulation by both a cellular transcription factor and a viral oncoprotein.  (+info)

Recruitment of the retinoblastoma protein to c-Jun enhances transcription activity mediated through the AP-1 binding site. (4/910)

The retinoblastoma susceptibility gene product (RB) is a transcriptional modulator. One of the targets for this modulator effect is the AP-1 binding site within the c-jun and collagenase promoters. The physical interactions between RB and c-Jun were demonstrated by co-immunoprecipitation of these two proteins using anti-c-Jun or anti-RB antisera, glutathione S-transferase affinity matrix binding assays in vitro, and electrophoretic mobility shift assays. The C-terminal site of the leucine zipper of c-Jun mediated the interaction with RB. Although the B-pocket domain of RB alone bound to c-Jun, a second c-Jun binding site in the RB was also suggested. Mammalian two-hybrid-based assay provided corroborative evidence that transactivation of gene expression by RB required the C-terminal region of c-Jun. We conclude that RB enhances transcription activity mediated through the AP-1 binding site. Adenovirus E1A or human papillomavirus E7 inhibits RB-mediated transcription activity. These data reveal that the interactions between these two distinct classes of oncoproteins RB and c-Jun may be involved in controlling cell growth and differentiation mediated by transcriptional regulation.  (+info)

Mutant p53 can provoke apoptosis in p53-deficient Hep3B cells with delayed kinetics relative to wild-type p53. (5/910)

Wild-type (wt) p53 frequently induces apoptosis when expressed in tumor cells whereas mutant p53 acts as an oncoprotein and consequently, stimulates cell proliferation. We report here exceptions to that rule. p53 conformational mutant 175H and DNA contact mutant 273H provoke apoptosis in human p53-deficient Hep3B hepatoma cells with delayed kinetics relative to wt p53. Similarly, c-Myc strongly stimulates apoptosis in these cells. In contrast, viral oncoproteins E1A and E7, and the cellular oncoprotein MDM-2, fail to elicit cytocidal responses. Efficient apoptotic cell death by mutant p53 requires oligomerization as 175H and 273H with deletions between amino acid residues 326 and 347 of the oligomerization domain are nontoxic. Apoptosis by mutant or wt p53 was significantly inhibited by the serine protease inhibitor AEBSF but not by the inactive analog AEBSA. Together, these results suggest that a wt p53-independent control mechanism is operational in Hep3B cells that eliminates cells upon sensing illegitimate proliferation signals originating from certain oncoproteins, including mutant p53 and Myc. We suggest that some tumor cell types lack p53 altogether because they tolerate neither wild-type nor mutant forms of the protein.  (+info)

Differences in the interactions of oncogenic adenovirus 12 early region 1A and nononcogenic adenovirus 2 early region 1A with the cellular coactivators p300 and CBP. (6/910)

Association with the cellular coactivators p300 and CBP is required for the growth-regulatory function of adenoviral (Ad) early region 1A (E1A) proteins. E1A regions necessary for these interactions overlap with domains involved in the induction of tumours in immunocompetent rodents through highly oncogenic Ad12. Differences in the association of cellular factors with the respective E1A domains of Ad12 and nononcogenic Ad2 might therefore be involved in serotype-specific oncogenicity. We analyzed the interaction of the Ad12 E1A 235R protein with p300 and CBP. Here we demonstrate that in the case of Ad12, but not Ad2/5, amino acids (aa) 1-29 of E1A proteins are sufficient to bind the p300-C/H3 domain in vivo and wild-type p300 in vitro. The conserved arginine-2, which is essential for the interaction between Ad2 E1A and p300, was dispensable for the Ad12 E1A 235R-p300 interaction in vitro. In addition to the p300-C/H3 region, we identified a second domain within p300 (aa 1999-2200) binding to the 235R protein. Contrary to p300, the amino-terminus and CR1 are necessary to associate with CBP. The aa 1-29 of the 235R protein but not CR1 are essential for the repression of colTRE-driven gene expression. This repression function is strictly dependent on p300 but not on CBP.  (+info)

Transcriptional regulation of the mouse ferritin H gene. Involvement of p300/CBP adaptor proteins in FER-1 enhancer activity. (7/910)

We previously identified a major enhancer of the mouse ferritin H gene (FER-1) that is central to repression of the ferritin H gene by the adenovirus E1A oncogene (Tsuji, Y., Akebi, N., Lam, T. K., Nakabeppu, Y., Torti, S. V., and Torti, F. M. (1995) Mol. Cell. Biol. 15, 5152-5164). To dissect the molecular mechanism of transcriptional regulation of ferritin H, E1A mutants were tested for their ability to repress FER-1 enhancer activity using cotransfection with ferritin H-chloramphenicol acetyltransferase (CAT) reporter constructs. Here we report that p300/CBP transcriptional adaptor proteins are involved in the regulation of ferritin H transcription through the FER-1 enhancer element. Thus, E1A mutants that failed to bind p300/CBP lost the ability to repress FER-1, whereas mutants of E1A that abrogated its interaction with Rb, p107, or p130 were fully functional in transcriptional repression. Transfection with E1A did not affect endogenous p300/CBP levels, suggesting that repression of FER-1 by E1A is not due to repression of p300/CBP synthesis, but to E1A and p300/CBP interaction. In addition, we have demonstrated that transfection of a p300 expression plasmid significantly activated ferritin H-CAT containing the FER-1 enhancer, but had a marginal effect on ferritin H-CAT with FER-1 deleted. Furthermore, both wild-type p300 and a p300 mutant that failed to bind E1A but retained an adaptor function restored FER-1 enhancer activity repressed by E1A. Sodium butyrate, an inhibitor of histone deacetylase, mimicked p300/CBP function in activation of ferritin H-CAT and elevation of endogenous ferritin H mRNA, suggesting that the histone acetyltransferase activity of p300/CBP or its associated proteins may contribute to the activation of ferritin H transcription. Recruitment of these broadly active transcriptional adaptor proteins for ferritin H synthesis may represent an important mechanism by which changes in iron metabolism are coordinated with other cellular responses mediated by p300/CBP.  (+info)

The adenoviral E1A oncoproteins interfere with the growth-inhibiting effect of the cdk-inhibitor p21(CIP1/WAF1). (8/910)

The cdk-inhibitor p21(CIP1/WAF1) inhibits the activities of cyclin-dependent kinases and proliferating cell nuclear antigen, thereby repressing cell-cycle progression and DNA replication. Transforming oncogenes, such as E1A of human adenovirus 5 (Ad5), may interfere with such growth-inhibitory proteins. In this study, we show that in various Ad5E1-transformed cells, p21(CIP1/WAF1) is expressed and that, in general, expression is not downregulated. In addition, colony-formation assays show that in Ad5E1-transformed cells highly overexpressed p21(CIP1/WAF1) can still cause growth inhibition. FACS experiments indicate, however, that a G1 arrest induced by moderate overexpression of p21(CIP1/WAF1) can be overcome by E1A. The E1A proteins may interfere with the function of p21(CIP1/WAF1) by binding. Indeed, p21(CIP1/WAF1) binds with its cyclin/cdk-binding N terminus to the transforming N-terminal and CR1 region of the E1A proteins. Together, these results lend support to the model that E1A can interfere directly with p21(CIP1/WAF1) function and thereby stimulates cell growth.  (+info)

Definition of Adenovirus E3 10.4K/14.5kD Protein in the Legal Dictionary - by Free online English dictionary and encyclopedia. What is Adenovirus E3 10.4K/14.5kD Protein? Meaning of Adenovirus E3 10.4K/14.5kD Protein as a legal term. What does Adenovirus E3 10.4K/14.5kD Protein mean in law?
In addition to decreased EGF-induced in vivo invasion of Mena11a-expressing cells, we also found that these cells express less CSF1 mRNA. Data from patients suggests that CSF1 and its receptor play crucial roles during progression of breast cancer (Kacinski et al., 1991; Scholl et al., 1994) and that CSF1 and the CSF1R are coexpressed in ,50% of breast tumors (Kacinski, 1997). Elevated circulating CSF1 was also suggested to be an indicator of early metastatic relapse in patients with breast cancer, independent of breast cancer subtype (Scholl et al., 1994; Tamimi et al., 2008; Beck et al., 2009). This suggests that lower levels of CSF1 in Mena11a-expressing cells could lead to decreased metastatic progression. The decreased invasion, intravasation and dissemination of Mena11a-expressing cells are consistent with the decrease in expression of CSF1 and the reduced sensitivity to EGF, which would make these cells less likely to participate in a paracrine signaling loop with macrophages.. A major ...
Adenovirus E1A induces cell proliferation, oncogenic transformation and promotes viral replication through interaction with p300/CBP, TRRAP/p400 multi-protein complex and the retinoblastoma (pRb) family proteins through distinct domains in the E1A N-terminal region. The C-terminal region of E1A suppresses E1A/Ras co-transformation and interacts with FOXK1/K2, DYRK1A/1B/HAN11 and CtBP1/2 (CtBP) protein complexes. To specifically dissect the role of CtBP interaction with E1A, we engineered a mutation (DL→AS) within the CtBP-binding motif, PLDLS, and investigated the effect of the mutation on immortalization and Ras cooperative transformation of primary cells and viral replication. Our results suggest that CtBP-E1A interaction suppresses immortalization and Ras co-operative transformation of primary rodent epithelial cells without significantly influencing the tumorigenic activities of transformed cells in immunodeficient and immunocompetent animals. During productive infection, CtBP-E1A ...
KRISP paper on Science 2018 on HLA and NK cells by Veron Ramsuran shows that HLA-B haplotypes that favor NKG2A-mediated NK cell licensing (i.e., education) exacerbate the deleterious effect of high HLA-A on HIV control, consistent with NKG2A-mediated inhibition impairing NK cell clearance of HIV-infected targets. Therapeutic blockade of HLA-E:NKG2A interaction may yield benefit in HIV disease.
Recombinant BCL2/adenovirus E1B 19kDa Interacting Protein 1 (BNIP1) Protein (GST tag). Species: Human. Source: Wheat germ. Order product ABIN1346798.
Ask1 activity was determined with an anti-P-Thr Ask1 antibody: a phospho-threonine antibody that targets the core of the Ask1 catalytic domain. This antibody showed increased staining in the discs co-expressing Pdk1:Akt and Ask1S83A compared with the Pdk1:Akt, Ask1WT and Pdk1:Akt, Ask1K618M discs (Fig. S1F-I,K). The co-expression of Pdk1:Akt and Ask1WT resulted in a very weak staining, although this was not statistically significant, suggesting that the ectopic expression of Akt attenuated P-Thr Ask1 levels (Fig. S1G). These results are consistent with previous observations showing a moderate increase in P-Thr Ask1 levels in UAS-Ask1WT-expressing cells and a strong increase in P-Thr Ask1 levels in the Ask1S83A-expressing cells, which concur with the role of Ser83 in the attenuation of Ask1 activity (Santabárbara-Ruiz et al., 2019). We propose here that this attenuation results in low levels of Ask1 activity that are still sufficient to activate p38. This is important for cell survival, as ...
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Reaktivität: Fledermaus, Rind (Kuh), Hund and more. 114 verschiedene BNIP3L Antikörper vergleichen. Alle direkt auf antikörper-online bestellbar!
Get an answer for Calculate Kc for the system, Ni2+ + Co Ni + Co2+. at 25 C?Ni2+ (aq) + 2e === Ni (s) E = - 0.25 V Co2+ (aq) + 2e === Co (s) E = - 0.28 and find homework help for other Science questions at eNotes
Molecular cloning and functional analysis of the adenovirus E1A-associated 300-kD protein (p300) reveals a protein with properties of a transcriptional adaptor ...
TY - JOUR. T1 - Repression of cytochrome P‐450c gene expression by cotransfection with adenovirus E1a DNA. AU - SOGAWA, Kazuhiro. AU - HANDA, Hiroshi. AU - FUJISAWA‐SEHARA, Atsuko. AU - HIROMASA, Takako. AU - YAMANE, Miyuki. AU - FUJII‐KURIYAMA, Yoshiaki. PY - 1989/5. Y1 - 1989/5. N2 - Gene expression of rat cytochrome P‐450c (P‐450c) depends upon inducible enhancers scattered in the 5′‐upstream region of the gene. We show that expression of the P‐450c gene is repressed by contransfection with adenovirus E1a DNA, regardless of the presence or absence of inducers, in a transient expression system of HeLa cells. Since cotransfection of either 13S or 12S E1a cDNA was effective in the repression, the region necessary for repression could be separated from that of transactivation of other adenovirus early genes. Moreover, we investigated the regions responsible for the inhibitory activity using in‐frame deletion mutants lacking internal or external portions of the E1a proteins. The ...
TY - JOUR. T1 - The adenovirus E4 11k protein binds and relocalizes the cytoplasmic P-body component Ddx6 to aggresomes. AU - Greer, Amy E.. AU - Hearing, Patrick. AU - Ketner, Gary W. PY - 2011/8/15. Y1 - 2011/8/15. N2 - The adenovirus E4 11k protein, product of E4 ORF3, is required in infection for processes including normal accumulation of viral late mRNAs. 11. k restructures both the nucleus and cytoplasm of infected cells by relocalizing specific host cell target proteins, most strikingly components of nuclear PML oncogenic domains. It is likely that in many cases relocalization inactivates target proteins to produce 11. ks effects, although the mechanism and targets for stimulation of late mRNA accumulation is unknown. We have identified a new set of proteins relocalized by 11. k: at least five protein components of cytoplasmic mRNA processing bodies (p-bodies) are found in 11. k-induced cytoplasmic aggresomes, sites where proteins are inactivated or destroyed. One of these p-body ...
The adenovirus E1B gene products are required for productive infection of human cells and for complete transformation of rodent cells in cooperation with the E1A gene products. Two major, unrelated polypeptides of 55,000 (55K) and 19,000 (19K) daltons are encoded by the E1B region. The 55K protein is required for efficient DNA replication, late mRNA transport to the cytoplasm and shut-off of cellular mRNA transport in productively infected cells. This protein is required for virus-mediated, but not DNA-mediated, transformation of rodent cells. It appears that the 55K protein does not directly contribute to cell transformation, but influences the oncogenicity of adenoviruses when they are inoculated into newborn hamsters. In contrast, the 19K protein is required for adenovirus induced cellular transformation and oncogenicity and localizes to membranes of the nuclear envelope, cytoplasm and the cell surface in transformed cells. This protein affects the efficiency of virus growth in some, but not ...
E1A oncogene expression sensitizes mammalian cells to apoptosis triggered by cytolytic lymphocytes (CL) [16]. Most studies suggest that E1A-induced apoptosis involves a p53-dependent cellular pathway that is blocked by the E1B 19 kDa gene product. In this study, the roles of p53 and E1B 19 kDa were …
HEK 293T/17 cells were transformed with adenovirus E1a carrying a temperature sensitive T antigen co-selected with neomycin. Transformation was brought about by the insertion of approximately 4.5 kilobases of viral genome into human chromosome 19.
HEK 293T/17 cells were transformed with adenovirus E1a carrying a temperature sensitive T antigen co-selected with neomycin. Transformation was brought about by the insertion of approximately 4.5 kilobases of viral genome into human chromosome 19.
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TY - JOUR. T1 - Conserved region 2 of adenovirus E1A has a function distinct from pRb binding required to prevent cell cycle arrest by p16(INK4a) or p27(Kip1). AU - Alevizopoulos, Konstantinos. AU - Sanchez, Belén. AU - Amati, Bruno. PY - 2000/4/13. Y1 - 2000/4/13. N2 - Ectopic expression of the CDK inhibitors (CKIs) p16(INK4a) and p27(Kip1) in Rat1 fibroblasts induces dephosphorylation and activation of Retinoblastoma-family proteins (pRb, p107 and p130), their association with E2F proteins, and cell cycle arrest in G1. The growth-inhibitory action of p16, in particular, is believed to be mediated essentially via pRb activation. The 12S E1A protein of human Adenovirus 5 associates with pRb-family proteins via residues in its Conserved Regions (CR) 1 and 2, in particular through the motif LXCXE in CR2. These interactions are required for E1A to prevent G1 arrest upon co-expression of CKIs. We show here that mutating either of two conserved motifs adjacent to LXCXE in CR2, GFP and SDDEDEE, also ...
Epithelial cells isolated from fresh human breast surgically resected tumor and normal margin. Cryopreserved samples available in frozen aliquots. High quality human breast primary epithelial cell cultures available for research.
2013 (English)In: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 110, no 50, 19976-19977 p.Article in journal, Editorial material (Other academic) Published ...
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Bcl-2/adenovirus E1B 19-kd interacting protein 3 (BNIP3) regulates hypoxia-induced neural precursor cell death. Journal of Neuropathology and Experimental Neurology. 68:1326-1338. 2009 ...
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Winnie Shum is the author of this article in the Journal of Visualized Experiments: Whole-cell Patch-clamp Recordings of Isolated Primary Epithelial Cells from the Epididymis
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Looking for online definition of adenovirus early region genes in the Medical Dictionary? adenovirus early region genes explanation free. What is adenovirus early region genes? Meaning of adenovirus early region genes medical term. What does adenovirus early region genes mean?
TY - JOUR. T1 - Adenovirus E1A protein activates transcription of the E1A gene subsequent to transcription complex formation. AU - Schaack, J.. AU - Logan, J.. AU - Vakalopoulou, E.. AU - Shenk, T.. PY - 1991. Y1 - 1991. N2 - The mechanism of transcriptional activation of the adenovirus E1A and E3 genes by E1A protein during infection was examined by using transcription-competition assays. Infection of HeLa cells with one virus led to inhibition of mRNA accumulation from a superinfecting virus. Synthesis of the E1A 289R protein by the first virus to infect reduced inhibition of transcription of the superinfecting virus, indicating that the E1A 289R protein was limiting for E1A-activated transcription. Infection with an E1A- virus, followed 6 h later by superinfection with a wild-type virus, led to preferential transcriptional activation of the E1A gene of the first virus, suggesting that a host transcription component(s) stably associated with the E1A promoter in the absence of E1A protein and ...
Adenovirus genomes are linear, non-segmented double-stranded (ds) DNA molecules that are typically 26-46 Kbp long, containing 23-46 protein-coding genes. The example used for the following description is Human adenovirus E, a mastadenovirus with a 36 Kbp genome containing 38 protein-coding genes. While the precise number and identity of genes varies among adenoviruses, the basic principles of genome organization and the functions of most of the genes described in this article are shared among all adenoviruses. The 38 genes in the Human Adenovirus E genome are organized in 17 transcription units, each containing 1-8 coding sequences. Alternative splicing during processing of the pre-mRNAs produced by each transcription unit enable multiple different mRNAs to be produced from one transcription unit. The E1A, E1B, E2A, E2B, E3, and E4 transcription units are successively transcribed early in the viral reproductive cycle. The proteins coded for by genes within these transcription units are mostly ...
The corepressor CtBP (carboxyl-terminal binding protein) is involved in transcriptional pathways important for development, cell cycle regulation, and transformation. We demonstrate that CtBP binding to cellular and viral transcriptional repressors is regulated by the nicotinamide adenine dinucleotides NAD+ and NADH, with NADH being two to three orders of magnitude more effective. Levels of free nuclear nicotinamide adenine dinucleotides, determined using two-photon microscopy, correspond to the levels required for half-maximal CtBP binding and are considerably lower than those previously reported. Agents capable of increasing NADH levels stimulate CtBP binding to its partners in vivo and potentiate CtBP-mediated repression. We propose that this ability to detect changes in nuclear NAD+/NADH ratio allows CtBP to serve as a redox sensor for transcription. ...
Intraperitoneal (i.p.) recurrence of cisplatin-refractory and p53 mutant ovarian cancer is a major clinical problem, despite surgery and chemotherapy. dl1520 (ONYX-015) is an E1B-55 kDa gene-deleted adenovirus engineered selectively to replicate in and destroy cancer cells lacking functional p53. Ho …
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Detect adenovirus rescue even before cytopathic effect (CPE) is observed. Ensure high titer by harvesting amplified adenovirus at the optimal time
Furthermore, neurons generated from other strains of mice as well as rats developed LB- and LN-like inclusions when treated with α-syn-hWT pffs, supporting the hypothesis. that induction of α-syn pathology is a general feature www.selleckchem.com/MEK.html of primary rodent neurons (data not shown). P-α-syn-positive aggregates (as detected by 81A) did not form in astrocytes (Figure S1B). Moreover, the appearance of α-syn pathology required the presence of endogenous α-syn since α-syn-hWT pffs did not induce any pathology in primary neurons from α-syn −/− mice (Figure 1C). Furthermore, monomeric α-syn did not induce α-syn inclusions (data not shown), demonstrating that α-syn pffs alone seed the aggregates. Immunoblot analyses were conducted on neuron lysates sequentially. extracted with 1% Tx-100, followed by 2% SDS (Figure 1B). In contrast to PBS-treated neurons, those treated with α-syn-hWT pffs for 14 days showed > 80% reduction of α-syn in the Tx-100-soluble fraction ...
Tangen JM, Fløisand Y, Foss-Abrahamsen J, Haukås E, Næss IA, Skjelbakken T. Overlevelse hos voksne med akutt myelogen leukemi. Tidsskr Nor Legeforen 2008;128(10):1164-7. Gardin C, Turlure P, Fagot T, Thomas X, Terre C, Contentin N, et…. ...
Orofaringe, just darrièr la boca. La paret anteriora es la basa de lenga e la vallècula; la paret laterala es formada per larc palatofaringi, larc palatoglós e per lamigdala palatina. La paret superiora es formada per luvula (campaneta) e lo paladar ...
TY - JOUR. T1 - Markedly enhanced cytolysis by E1B-19kD-deleted oncolytic adenovirus in combination with cisplatin. AU - Yoon, A. Rum. AU - Kim, Joo Hang. AU - Lee, Young Sook. AU - Kim, Hoguen. AU - Yoo, Ji Young. AU - Sohn, Joo Hyuk. AU - Park, Byeong Woo. AU - Yun, Chae Ok. PY - 2006/4/1. Y1 - 2006/4/1. N2 - Oncolytic adenoviruses are currently being developed as novel antitumor therapeutics. To enhance their therapeutic potential, adenoviruses are being administered in combination with standard chemotherapy. Adenoviral vectors used in these clinical trials, however, can be destructive as they encode intact E1B 19-kDa protein, which can block the apoptotic pathway induced by a variety of chemotherapeutic agents. Previously, we have shown that oncolytic adenovirus Ad-ΔE1B19/55, deleted for sequence encoding E1B 19-kDa and E1B 55-kDa proteins, exhibits marked enhancement in cytolytic and apoptotic activity [Kim, J., Cho, J.Y., Kim, J.H., Jung, K.C., and Yun, C.O. (2002). Cancer Gene Ther. 9, ...
TY - JOUR. T1 - Etoposide enhances antitumor efficacy of MDR1-driven oncolytic adenovirus through autoupregulation of the MDR1 promoter activity. AU - Su, Bing Hua. AU - Shieh, Gia Shing. AU - Tseng, Yau Lin. AU - Shiau, Ai Li. AU - Wu, Chao Liang. PY - 2015/1/1. Y1 - 2015/1/1. N2 - Conditionally replicating adenoviruses (CRAds), or oncolytic adenoviruses, such as E1B55K-deleted adenovirus, are attractive anticancer agents. However, the therapeutic efficacy of E1B55K-deleted adenovirus for refractory solid tumors has been limited. Environmental stress conditions may induce nuclear accumulation of YB-1, which occurs in multidrug-resistant and adenovirus-infected cancer cells. Overexpression and nuclear localization of YB-1 are associated with poor prognosis and tumor recurrence in various cancers. Nuclear YB-1 transactivates the multidrug resistance 1 (MDR1) genes through the Y-box. Here, we developed a novel E1B55K-deleted adenovirus driven by the MDR1 promoter, designed Ad5GS3. We tested the ...
locus, play critical roles in inducing and maintaining permanent cell cycle arrest during cellular senescence. Although the elimination of p16INK4a-expressing cells extends the life span of the mouse, it is unclear whether tissue function is restored by the elimination of senescent cells in aged animals and whether and how p19ARF contributes to tissue aging. The aging-associated decline in lung function is characterized by an increase in compliance as well as pathogenic susceptibility to pulmonary diseases. We herein demonstrated that pulmonary function in 12-month-old mice was reversibly restored by the elimination of p19ARF-expressing cells. The ablation of p19ARF-expressing cells using a toxin receptor-mediated cell knockout system ameliorated aging-associated lung hypofunction. Furthermore, the aging-associated gene expression profile was reversed after the elimination of p19ARF. Our results indicate that the aging-associated decline in lung function was, at least partly, attributed to ...
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Adenovirus Type 9, 0.1 mg. The many different serotypes of human adenoviruses (Ad) are divided into six subgroups, of which all Ad subgroup A and B and two subgroup D Ads can elicit tumors in infected rodents.
Our study is the first report of an RSAd, in which the promoter-based regulation of E1A, approach is used to target the deregulated G1 to S phase in tumor cells. We demonstrated that AdE2F-1RC replicated selectively in tumor cells and not in normal cells expressing high and low levels of E2F-1 protein, respectively. Additionally, in two mouse xenograft models, AdE2F-1RC exhibited significant in vivo therapeutic benefit often equivalent to wild-type adenovirus treatment. These studies validate several design features of AdE2F-1RC.. The wild-type adenovirus dl309 replicated in all of the normal cells tested. We reasoned that normal resting cells would be a good model for AdE2F-1RC toxicity tests, because these cells do not express E2F-1 (44 , 45) and are found in the tumor environment. In contrast to dl309, the replication and CPE of AdE2F-1RC was significantly attenuated in normal cells suggesting that the E2F-1 promoter was not optimally activated. One reason is that the presence of pRb/E2F-1 ...
People get rats from lots of different places and they arent always the tamest of rats. There is no point telling someone who has just picked up a couple of unhandled babies that they ought to have looked for a breeder who selects for calm temperament and handles their baby rats daily. First, they probably tried that but didnt want to wait 4-6 months for baby rats from these (frankly rare) breeders who handle daily. Second, its too late!. However, the good news is that for most rats, taming is just a matter of time and patience. While it may not seem easy, it is definitely possible to bring a rat around from a skittish scared baby to a great pet. In fact, some of the nicest adult rats Ive had started out as not very confident babies.. There are different ideas about taming rats. Whatever method you choose, Id suggest that early on (immediately) stop chasing your rats around a cage. The more you chase, the more theyll run and you are encouraging them into a habit of running away from you. ...
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This post is about adenovirus infection, a major cause of illness both minor and severe in the United States, especially among children in group settings.
101721 - Circunferência escrotal e medidas morfométricas em cordeiros da raça Santa Inês e F1 Santa Inês X Dorper - Scrotal circumference and morphometric measurements in lambs Santa Inês and F1 Dorper x Santa Inês | Veterinaria.org . La primera comunidad veterinaria de habla hispana con presencia en Espa a y Am rica del Sur.
MT -BOX CABLES FOR SALE IN STOCK- COMBO QUALITY IT IS USED BOTH FOR MT AND JAF BOX 6125 5300 6300 6260S E 66 7510-S E 63 5610 E 75 6500-S 5800 6208-C 7610-S 1202 3610-F 2680-C 3120-C 3600-S N 73 6220-C 6303-C 1200 E 90 7210-C 2630 E 71 N 95-8GB N 81 OLD EASY NEW EASY 5500 5310-XM 1680-C 7500 N 78 6500-C 7100-S N 70 N 82 5630-D ...
Společnost PANBEX hledala řešení pro řízení výkonnosti, které by jí pomohlo zkvalitnit stávající holdingové procesy pro finanční a účetní konsolidaci. Do té doby muselo finanční oddělení mateřské firmy každý měsíc konsolidovat potřebná data s velkou pracovní a časovou náročností. Výkazy z jednotlivých poboček bylo nutné naplnit do předpřipravených souborů a poté manuálně převést do centrálního konsolidačního softwaru postaveném na aplikaci Excel. Vedle pracnosti byl celý systém velmi náchylný na vznik chyb. Po zavedení řešení Infor PM se díky automatizaci konsolidačních procesů podařilo redukovat dobu potřebnou na vytváření skupinových reportů z jednoho týdne na půl dne ...
The RAPAd® method of Adenovirus construction, developed by ViraQuest Inc. scientists, has been used by other scientists around the world.. Request Quote ...
U n i S e t . _ _ i n i t _ _ def __init__(self, node): Constructor. self.name = node.attrib[NAME_A] self.fpi = node.attrib.get(FPI_A, None ...
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Lillie, J. W.; Loewenstein, P. M.; Green, M. R.; Green, M. (1987). "Functional domains of adenovirus type 5 E1a proteins". Cell ... He discovered two of the proteins required for adenoviruses to transform cells, and also other proteins that usurp the infected ... In recent years, Green has focused his studies on the multifunctional adenovirus oncoprotein E1A, which is necessary for cell ... Loewenstein, P. M., Wu, S., Chiang, C., & Green, M. (2012). The adenovirus E1A N-terminal repression domain represses ...
Whyte, P (1988). "Association between an oncogene and an anti-oncogene: the adenovirus E1A proteins bind to the retinoblastoma ... "Association between an oncogene and an anti-oncogene: the adenovirus E1A proteins bind to the retinoblastoma gene product." ( ... Whyte, P (1989). "Cellular targets for transformation by the adenovirus E1A proteins". Cell. 56: 67-75. doi:10.1016/0092-8674( ... "Cellular targets for transformation by the adenovirus E1A proteins." cell 56.1 (1989): 67-75. National Academy of Sciences, ...
"Mammalian Srb/Mediator complex is targeted by adenovirus E1A protein". Nature. 399 (6733): 276-9. Bibcode:1999Natur.399..276B. ... Nakayama M, Kikuno R, Ohara O (Nov 2002). "Protein-protein interactions between large proteins: two-hybrid screening using a ... This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which ... Ko L, Cardona GR, Chin WW (May 2000). "Thyroid hormone receptor-binding protein, an LXXLL motif-containing protein, functions ...
The protein encoded by this gene was first identified by its ability to bind the adenovirus E1A protein. The protein localizes ... a novel adenovirus E1A-associated protein that inhibits E1A transactivation". The EMBO Journal. 14 (13): 3159-69. doi:10.1002/j ... Masselink H, Bernards R (Mar 2000). "The adenovirus E1A binding protein BS69 is a corepressor of transcription through ... "Ubiquitin-dependent degradation of adenovirus E1A protein is inhibited by BS69". Biochemical and Biophysical Research ...
Li Y, Graham C, Lacy S, Duncan AM, Whyte P (1993). "The adenovirus E1A-associated 130-kD protein is encoded by a member of the ... "Interaction between human cyclin A and adenovirus E1A-associated p107 protein". Science. 255 (5040): 87-90. Bibcode:1992Sci... ... Cyclin A2 mediates the abundance of RAD51 protein by inhibiting proteasome degradation of this protein. During mouse ... Cyclin-A2 is a protein that in humans is encoded by the CCNA2 gene. It is one of the two types of cyclin A: cyclin A1 is ...
"Adenovirus E1A proteins direct subcellular redistribution of Nek9, a NimA-related kinase". Journal of Cellular Physiology. 212 ... "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology. 3: 89. doi:10.1038/ ... "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology. 3 (1): 89. doi: ... Serine/threonine-protein kinase Nek9 is an enzyme that in humans is encoded by the NEK9 gene. NEK9 has been shown to interact ...
This protein can influence splice site selection of adenovirus E1A pre-mRNA. It interacts with the oncoprotein TLS, and ... FUS-interacting serine-arginine-rich protein 1 is a protein that in humans is encoded by the SFRS13A gene. This gene product is ... "TLS-ERG leukemia fusion protein inhibits RNA splicing mediated by serine-arginine proteins". Mol. Cell. Biol. 20 (10): 3345-54 ... "TLS-ERG leukemia fusion protein inhibits RNA splicing mediated by serine-arginine proteins". Mol. Cell. Biol. 20 (10): 3345-54 ...
Histone acetyltransferase p300 also known as p300 HAT or E1A-associated protein p300 (where E1A = adenovirus early region 1A) ... "Relief of YY1 transcriptional repression by adenovirus E1A is mediated by E1A-associated protein p300". Genes Dev. 9 (10): 1188 ... "Molecular cloning and functional analysis of the adenovirus E1A-associated 300-kD protein (p300) reveals a protein with ... This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. EP300 is closely related to ...
Hateboer G, Hijmans EM, Nooij JB, Schlenker S, Jentsch S, Bernards R (1996). "mUBC9, a novel adenovirus E1A-interacting protein ... For example, sumoylation may affect a protein's localization in the cell, its ability to interact with other proteins or DNA. ... SENP proteases can remove SUMO from sumoylated proteins, freeing it to be used in further sumoylation reactions. The protein ... Four alternatively spliced transcript variants encoding the same protein have been found for this gene. The UBC9 protein ...
"Relief of YY1 transcriptional repression by adenovirus E1A is mediated by E1A-associated protein p300". Genes & Development. 9 ... "Relief of YY1 transcriptional repression by adenovirus E1A is mediated by E1A-associated protein p300". Genes & Development. 9 ... and relief of repression by adenovirus E1A protein". Cell. 67 (2): 377-88. doi:10.1016/0092-8674(91)90189-6. PMID 1655281. ... YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein ...
"Upregulation of the Golgi protein GP73 by adenovirus infection requires the E1A CtBP interaction domain". Virology. 301 (2): ... Golgi membrane protein 1 (GOLM1) also known as Golgi phosphoprotein 2 or Golgi membrane protein GP73 is a protein that in ... The protein encoded by this gene is a type II Golgi transmembrane protein. It processes protein synthesized in the rough ... Golgi membrane protein 1 is overexpressed in prostate cancer and lung adenocarcinoma tissue. Blood levels of GP73 are higher in ...
Pines, Jonathon; Hunter, Tony (1990). "Human cyclin A is adenovirus E1A-associated protein p60 and behaves differently from ... "the discovery of protein kinases that phosphorylate tyrosine residues in proteins, critical for the regulation of a wide ... "Minichromosome Maintenance Proteins Interact with Checkpoint and Recombination Proteins to Promote S-Phase Genome Stability". ... Hunter, Anthony Rex (1969). Aspects of mammalian protein synthesis (PhD thesis). University of Cambridge. OCLC 500479081. "Tony ...
A divergent MYND domain present in the adenovirus E1A binding protein BS69 was also shown to interact with N-CoR and mediate ... Masselink H, Bernards R (March 2000). "The adenovirus E1A binding protein BS69 is a corepressor of transcription through ... The current evidence suggests that the MYND motif in mammalian proteins constitutes a protein-protein interaction domain that ... protein. ETO has been shown to be a co-repressor recruited by the promyelocytic leukemia zinc finger (PLZF) protein. ...
Masselink H, Bernards R (2000). "The adenovirus E1A binding protein BS69 is a corepressor of transcription through recruitment ... is a protein that in humans is encoded by the NCOR1 gene. NCOR1 is a transcriptional coregulatory protein which contains ... "Both corepressor proteins SMRT and N-CoR exist in large protein complexes containing HDAC3". EMBO J. 19 (16): 4342-50. doi: ... It is a member of the family of nuclear receptor corepressors; the other human protein that is a member of that family is ...
Pines, Jonathon; Hunter, Tony (1990). "Human cyclin A is adenovirus E1A-associated protein p60 and behaves differently from ... and identified the first link between cyclins and oncoproteins by showing that cyclin A bound to adenovirus E1A, thus linking ... proteolysis coordinates chromosome congression with cytokinesis and mitotic exit by degrading specific proteins at specific ...
Cellular Repressor of E1A-stimulated Genes 1) is a protein that in humans is encoded by the CREG1 gene. The adenovirus E1A ... The protein encoded by this gene antagonizes transcriptional activation and cellular transformation by E1A. This protein shares ... Jiang LQ, Wen SJ, Wang HY, Chen LY (2003). "Screening the proteins that interact with calpain in a human heart cDNA library ... 2003). "The Secreted Protein Discovery Initiative (SPDI), a Large-Scale Effort to Identify Novel Human Secreted and ...
E1A adenovirus protein, and S-HDAg (hepatitis delta virus small delta antigen). p300/CBP have also been observed to acetylate β ... and E1A adenovirus protein. It can also autoacetylate, which facilitates intramolecular interactions with its bromodomain that ... structural proteins, polyamines, and proteins involved in nuclear import. Acetylation of these proteins can alter their ability ... Histones comprise the protein portion of chromatin. There are five different histone proteins: H1, H2A, H2B, H3, and H4. A core ...
pRb is one of the targets of the oncogenic human papilloma virus protein E7, and human adenovirus protein E1A. By binding to ... Most E2F have a pocket protein binding domain. Pocket proteins such as pRB and related proteins p107 and p130, can bind to E2F ... Homo sapiens E2F1 mRNA or E2F1 protein sequences from NCBI protein and nucleotide database. X-ray crystallographic analysis has ... Activator E2F proteins can then transcribe S phase promoting genes. In REF52 cells, overexpression of activator E2F1 is able to ...
The adenovirus E1B protein (55K) prevents p53 from regulating genes by binding to the site on p53 which binds to the genome. In ... The adenovirus early region 1A (E1A) is an oncoprotein which binds to Rb and can stimulate transcription and transform cells. ... HPV instead degrades p53: the HPV protein E6 binds to a cellular protein called the E6-associated protein (E6-AP, also known as ... DNA oncoviruses typically impair two families of tumor suppressor proteins: tumor proteins p53 and the retinoblastoma proteins ...
"Isolation of a cDNA encoding the adenovirus E1A enhancer binding protein: a new human member of the ets oncogene family". ... "Entrez Gene: ETV4 Ets variant gene 4 (E1A enhancer binding protein, E1AF)". "ETV4 Gene - GeneCards , ETV4 Protein , ETV4 ... encoding the adenovirus E1A enhancer-binding protein, in extraosseous Ewing's sarcoma". Biochemical and Biophysical Research ... ETV4+protein,+human at the US National Library of Medicine Medical Subject Headings (MeSH) Overview of all the structural ...
... the Adenovirus protein E1A. Pathogenic bacteria also mimic host motifs (as well as having their own motifs), however, not to ... linear motifs or minimotifs are short stretches of protein sequence that mediate protein-protein interaction. The first ... Linear motif mediated protein-protein interactions have shown promise in recent years as novel drug targets. Success stories ... Beuming T, Skrabanek L, Niv MY, Mukherjee P, Weinstein H (March 2005). "PDZBase: a protein-protein interaction database for PDZ ...
The CtBP1 protein was originally identified as a human protein that bound a PLDLS motif in the C-terminus of adenovirus E1A ... 1993). "A region in the C-terminus of adenovirus 2/5 E1a protein is required for association with a cellular phosphoprotein and ... 1998). "Interaction between a cellular protein that binds to the C-terminal region of adenovirus E1A (CtBP) and a novel ... "Interaction between a cellular protein that binds to the C-terminal region of adenovirus E1A (CtBP) and a novel cellular ...
... and relief of repression by adenovirus E1A protein". Cell. 67 (2): 377-88. doi:10.1016/0092-8674(91)90189-6. PMID 1655281. ... Furthermore, SINEs frequently contain motifs for YY1 polycomb proteins. YY1 is a zinc-finger protein that acts as a ... Thereafter, one of the strands is incorporated into a multi-protein RNA-induced silencing complex (RISC). Among these proteins ... encodes a protein which binds to RNA and acts as a chaperone to facilitate and maintain the LINE protein-RNA complex structure ...
... a zinc-finger protein that shares an epitope with the adenovirus E1A protein". Proceedings of the National Academy of Sciences ... PR domain zinc finger protein 2 is a protein that in humans is encoded by the PRDM2 gene. This tumor suppressor gene is a ... It encodes a zinc finger protein that can bind to retinoblastoma protein, estrogen receptor, and the TPA-responsive element ( ... PRDM2+protein,+human at the US National Library of Medicine Medical Subject Headings (MeSH) This article incorporates text from ...
... replication of the adenovirus genome can occur. A terminal protein that is covalently bound to the 5' end of the adenovirus ... E1A (binds Retinoblastoma tumor suppressor protein) has been found to immortalize primary cells in vitro allowing E1B (binds ... Adenoviruses Stanford University-Adenoviruses Adenoviruses General Concepts General information on Adenovirus DNA virus ... Tupaia adenovirus (TAV) (tree shrew adenovirus 1) has been isolated from tree shrews. Otarine adenovirus 1 has been isolated ...
The protein is differentially regulated by E1A-induced phosphorylation. The full-length gene product represses transcription ... Fernandes ER, Rooney RJ (1997). "The adenovirus E1A-regulated transcription factor E4F is generated from the human homolog of ... Transcription factor E4F1 is a protein that in humans is encoded by the E4F1 gene. The zinc finger protein encoded by this gene ... "Chromosomal location and tissue expression of the gene encoding the adenovirus E1A-regulated transcription factor E4F in humans ...
Li Y, Graham C, Lacy S, Duncan AM, Whyte P (1994). "The adenovirus E1A-associated 130-kD protein is encoded by a member of the ... Li Y, Graham C, Lacy S, Duncan AM, Whyte P (December 1993). "The adenovirus E1A-associated 130-kD protein is encoded by a ... This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to ... G1/S-specific cyclin-E1 is a protein that in humans is encoded by the CCNE1 gene. The protein encoded by this gene belongs to ...
"The carboxy-terminal region of adenovirus E1A activates transcription through targeting of a C-terminal binding protein-histone ... Retinoblastoma protein, Retinoblastoma-like protein 1, Retinoblastoma-like protein 2, SAP30, SATB1, SIN3A, SIN3B, SPEN, SUDS3, ... Yao YL, Yang WM (October 2003). "The metastasis-associated proteins 1 and 2 form distinct protein complexes with histone ... the polycomb group proteins HPC2 and BMI-1, and the corepressor C-terminal-binding protein". Proceedings of the National ...
"Adenovirus E1A, simian virus 40 tumor antigen, and human papillomavirus E7 protein share the capacity to disrupt the ... "A family of proteins structurally and functionally related to the E6-AP ubiquitin-protein ligase". Proceedings of the National ... 1990 Huibregtse, J.M.; Scheffner, M.; Howley, P.M. (1991). "A cellular protein mediates association of p53 with the E6 ... 1989 Werness, B.; Levine, A.; Howley, P. (1990). "Association of human papillomavirus types 16 and 18 E6 proteins with p53". ...
Interestingly, adenovirus has an E1A protein to induce apoptosis by initiating the cell cycle, and an E1B protein to block the ... White E (1998). "Regulation of Apoptosis by Adenovirus E1A and E1B Oncogenes". Seminars in Virology. 8 (6): 505-513. doi: ... proteins, DNA and/or RNA. Namely, viral proteins of herpes simplex virus can degrade host DNA and inhibit host cell DNA ... In most cases, tropism is determined by the ability of the viral surface proteins to fuse or bind to surface receptors of ...
"Interaction between a cellular protein that binds to the C-terminal region of adenovirus E1A (CtBP) and a novel cellular ... Retinoblastoma-binding protein 8 is a protein that in humans is encoded by the RBBP8 gene. The protein encoded by this gene is ... "Interaction between a cellular protein that binds to the C-terminal region of adenovirus E1A (CtBP) and a novel cellular ... It is found among several proteins that bind directly to retinoblastoma protein, which regulates cell proliferation. This ...
... adenovirus e1a proteins MeSH D12.776.964.700.045.050.110 - adenovirus e1b proteins MeSH D12.776.964.700.045.060 - adenovirus e2 ... adenovirus E1 proteins MeSH D12.776.624.664.520.045.050.100 - adenovirus E1A proteins MeSH D12.776.624.664.520.045.050.110 - ... adenovirus e2 proteins MeSH D12.776.624.664.520.045.070 - adenovirus e3 proteins MeSH D12.776.624.664.520.045.080 - adenovirus ... adenovirus e3 proteins MeSH D12.776.964.700.045.080 - adenovirus e4 proteins MeSH D12.776.964.700.750.320 - fusion proteins, ...
Control protein E1A activates transcription of a number of viral genes as well as genes of the host cell. Control protein E1B ... The functions of many adenovirus proteins are known: Structural proteins include capsid proteins II (hexon), III (penton base ... "Protein Details for Human adenovirus E". NCBI. Retrieved 2013-01-17. Russell, WC (Jan 2009). "Adenoviruses: update on structure ... and the terminal protein TP. Encapsidation proteins IVa2, 52K, and L1, and hexon assembly protein 100K are involved in assembly ...
Li Y, Graham C, Lacy S, Duncan AM, Whyte P (Dec 1993). "The adenovirus E1A-associated 130-kD protein is encoded by a member of ... Li Y, Graham C, Lacy S, Duncan AM, Whyte P (Dec 1993). "The adenovirus E1A-associated 130-kD protein is encoded by a member of ... Retinoblastoma-like protein 2 is a protein that in humans is encoded by the RBL2 gene. Retinoblastoma-like protein 2 has been ... "The HMG-box transcription factor HBP1 is targeted by the pocket proteins and E1A". Oncogene. 14 (22): 2721-8. doi:10.1038/sj. ...
... whose presence correlates with activation of RNA polymerase III-mediated transcription by adenovirus E1A expression and serum ... "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173-8. Bibcode:2005Natur. ... GTF3C2+protein,+human at the US National Library of Medicine Medical Subject Headings (MeSH) FactorBook TFIIIC-110 This article ... General transcription factor 3C polypeptide 2 is a protein that in humans is encoded by the GTF3C2 gene. GTF3C2 has been shown ...
... cloning and characterization of a cellular phosphoprotein that interacts with a conserved C-terminal domain of adenovirus E1A ... C-terminal-binding protein 2 also known as CtBP2 is a protein that in humans is encoded by the CTBP2 gene. The CtBPs - CtBP1 ... "The LIM protein FHL3 binds basic Krüppel-like factor/Krüppel-like factor 3 and its co-repressor C-terminal-binding protein 2". ... "C-Terminal binding protein is a transcriptional repressor that interacts with a specific class of vertebrate Polycomb proteins ...
Tsai LH, Harlow E, Meyerson M (September 1991). "Isolation of the human cdk2 gene that encodes the cyclin A- and adenovirus E1A ... The protein encoded by this gene is a member of the cyclin-dependent kinase family of Ser/Thr protein kinases. This protein ... This increases the synthesis of histone proteins (the major protein component of chromatin), and subsequently supports the DNA ... where cells make proteins necessary for mitosis and replicate their DNA. This protein associates with and is regulated by the ...
"Regulation of the 26S proteasome by adenovirus E1A". EMBO J. 19 (17): 4759-73. doi:10.1093/emboj/19.17.4759. PMC 302057. PMID ... To recognize protein as designated substrate, 19S complex has subunits that are capable to recognize proteins with a special ... Accordingly, misfolded proteins and damaged protein need to be continuously removed to recycle amino acids for new synthesis; ... The 19S regulatory particles can recognize ubiquitin-labeled protein as degradation substrate, unfold the protein to linear, ...
SV40 large TAg, other polyomavirus large T antigens, adenovirus E1a proteins, and oncogenic human papillomavirus E7 proteins ... Protein-protein interactions between T-antigen and DNA polymerase-alpha directly stimulate replication of the virus genome. T- ... "The binding domain structure of retinoblastoma-binding proteins". Protein Science. 2 (2): 155-64. doi:10.1002/pro.5560020204. ... The SV40 large T-antigen has been used as a model protein to study nuclear localization signals (NLSs). It is imported into the ...
... the host cell must be induced into S phase by viral proteins interfering with cell cycle proteins. The adenoviral E1A gene is ... and the knob domain of the adenovirus coat protein trimer. CAR is necessary for adenovirus infection. Although expressed widely ... E1B proteins produced by adenoviruses protect the infected cell by binding to and degrading the p53 transcription factors, ... These molecules are fusion proteins that are made up of an antibody raised against the knob domain of the adenovirus coat ...
... a zinc-finger protein that shares an epitope with the adenovirus E1A protein". Proceedings of the National Academy of Sciences ... The retinoblastoma protein (protein name abbreviated pRb; gene name abbreviated Rb, RB or RB1) is a proto-oncogenic tumor ... E2F1 to E2F5 are known to associate with proteins in the pRb-family of proteins while E2F6 and E2F7 are independent of pRb. ... RB/E2F-family proteins repress transcription. pRb is a multifunctional protein with many binding and phosphorylation sites. ...
Both the RB1 protein and the product of this gene can form a complex with adenovirus E1A protein and SV40 Large T-antigen, with ... "Interaction between human cyclin A and adenovirus E1A-associated p107 protein". Science. 255 (5040): 87-90. Bibcode:1992Sci... ... "Reverse two-hybrid and one-hybrid systems to detect dissociation of protein-protein and DNA-protein interactions". Proceedings ... Retinoblastoma-like 1 (p107), also known as RBL1, is a protein that in humans is encoded by the RBL1 gene. The protein encoded ...
The adenovirus early region 1A (E1A) proteins were described originally as immortalizing oncoproteins that altered ... Surprisingly, the 243-amino-acid form of adenovirus-5 E1A was found subsequently to reverse-transform many human tumour cells. ... E1A) proteins were described originally as immortalizing oncoproteins that altered transcription in rodent cells, but ... Tumour suppression apparently results from the ability of E1A to re-programme transcription in tumour cells, and the molecular ...
Muller U, Roberts MP, Engel DA, Doerfler W, Shenk T. Induction of transcription factor AP-1 by adenovirus E1A protein and cAMP ... Induction of transcription factor AP-1 by adenovirus E1A protein and cAMP. In: Genes and Development. 1989 ; Vol. 3, No. 12 A. ... keywords = "AP-1, adenovirus E1A protein, cAMP, transcriptional control",. author = "U. Muller and Roberts, {M. P.} and Engel ... Induction of transcription factor AP-1 by adenovirus E1A protein and cAMP. / Muller, U.; Roberts, M. P.; Engel, D. A. et al. ...
Lillie, J. W.; Loewenstein, P. M.; Green, M. R.; Green, M. (1987). "Functional domains of adenovirus type 5 E1a proteins". Cell ... He discovered two of the proteins required for adenoviruses to transform cells, and also other proteins that usurp the infected ... In recent years, Green has focused his studies on the multifunctional adenovirus oncoprotein E1A, which is necessary for cell ... Loewenstein, P. M., Wu, S., Chiang, C., & Green, M. (2012). The adenovirus E1A N-terminal repression domain represses ...
E1A). An adenovirus starts producing copies of E1A shortly after it infects a cell. E1A proteins interact with a variety of key ... First, like many folded proteins, E1A turns out to employ a basic regulatory mechanism called allostery: when one protein ... E1A is worth studying not just because it facilitates adenovirus infections, but also because its a prime example of an IDP. ... "So now you multiply the complexity-and you can see how proteins such as E1A manage to achieve so much so quickly within a cell ...
... results in the inhibition of adenovirus virus-associated (VA) gene transcription in vitro. The involvement of PKC in this ... We report here that activation of protein kinase C (PKC) ... A purified adenovirus 289-amino-acid E1A protein activates RNA ... Hoeffler W. K., Kovelman R., Roeder R. G. 1988; Activation of transcription factor IIIC by adenovirus E1A protein. Cell 53:907- ... Activation of protein kinase C inhibits adenovirus VA gene transcription in vitro * Calvin B. L. James1, Timothy H. Carter2 ...
The adaptor protein DCAF7. mediates the interaction of the adenovirus E1A. oncoprotein with the protein kinases DYRK1A. and ... Development of a sensitive non-radioactive protein kinase assay and its application for detecting DYRK activity in Xenopus ... Differential maturation and chaperone dependence of the paralogous protein kinases DYRK1A. and DYRK1B., Papenfuss M, Lützow S, ... protein., Qu Y, Adler V, Izotova L, Pestka S, Bowne W, Michl J, Boutjdir M, Friedman FK, Pincus MR., Front Biosci. September 1 ...
We show that the adenovirus E1A protein inhibits NO production by decreasing expression of the inducible NO synthase (NOS2). ... We show that the adenovirus E1A protein inhibits NO production by decreasing expression of the inducible NO synthase (NOS2). ... We show that the adenovirus E1A protein inhibits NO production by decreasing expression of the inducible NO synthase (NOS2). ... We show that the adenovirus E1A protein inhibits NO production by decreasing expression of the inducible NO synthase (NOS2). ...
The zinc finger protein GLI transforms primary cells in cooperation with adenovirus E1A. Mol Cell Biol ... 1-3). HH signaling is initiated by the binding of HH protein to its receptor Patched (PTCH), a transmembrane domain protein ... In our studies, EGFR did not affect the levels of GLIact proteins (see Fig. 3C; data not shown) and the synergistic activation ... Our data on EGFR and HH/GLI integration also support the notion that GLI proteins act as an information nexus to integrate ...
These primary cell cultures were often noted to spontaneously degenerate over time, and adenoviruses are now known to be a ... Adenovirus, a DNA virus, was first isolated in the 1950s in adenoid tissue-derived cell cultures, hence the name. ... Thereafter, adenovirus produces potent E1A proteins that immortalize primary rodent cells by altering cellular transcription, ... Adenovirus became a significant cause of economic cost and morbidity in this setting. A live oral vaccine against adenovirus ...
The adenovirus E1A-associated 130-kD protein is encoded by a member of the retinoblastoma gene family and physically interacts ... Association between an oncogene and an anti-oncogene: the adenovirus E1A proteins bind to the retinoblastoma gene product. 1988 ... Licensing of DNA replication by a multi-protein complex of MCM/P1 proteins in Xenopus eggs. 1997, Pubmed , Xenbase Kubota, ... The P1 family: a new class of nuclear mammalian proteins related to the yeast Mcm replication proteins. 1994, Pubmed Hu, ...
This gene encodes a protein that binds to the C-terminus of adenovirus E1A proteins. This phosphoprotein is a transcriptional ... This protein and the product of a second closely related gene, CTBP2, can dimerize. Both proteins can also interact with a ... polycomb group protein complex which participates in regulation of gene expression during development. Alternative splicing of ...
BS69, an adenovirus E1A-associated protein binds to and inhibits the transcriptional activity of c-Myb Oncogene Lipsick, J.S, ... BS69, an adenovirus E1A-associated protein, inhibits the transcriptional activity of c-Myb ONCOGENE Ladendorff, N. E., Wu, S., ... A yeast two-hybrid screen identified BS69, an adenovirus E1A-associated protein, as interacting with the carboxy-terminal ... A direct interaction between these two proteins was observed in vitro. Furthermore, the 289R E1A protein could inhibit the BS69 ...
p400 is a cellular protein which binds to the adenovirus E1A oncoprotein(s). The biological function of p400 is not yet known, ... p400 is a cellular protein which binds to the adenovirus E1A oncoprotein(s). The biological function of p400 is not yet known, ... p400 is a cellular protein which binds to the adenovirus E1A oncoprotein(s). The biological function of p400 is not yet known, ... p400 is a cellular protein which binds to the adenovirus E1A oncoprotein(s). The biological function of p400 is not yet known, ...
C-terminal binding proteins (CtBP1 and CtBP2) are a class of co-repressors that interact with a subset of transcription factors ... C-terminal binding proteins (CtBP1 and CtBP2) are a class of co-repressors that interact with a subset of transcription factors ... Transcriptional repression is critical for genetic regulation of many cellular proteins, and involves either a transcription ... Transcriptional repression is critical for genetic regulation of many cellular proteins, and involves either a transcription ...
This protein is thought to bind to the C-terminus of the adenovirus E1A proteins. Studies in mice suggested that this protein ... C-Terminal binding protein 2 (CTBP2) is a transcriptional repressor. It contains a NAD+ binding domain similar to NAD+- ...
... induction of apoptosis by adenovirus type 2 (Ad2) early region 4 ORF 4 (E4orf4) correlates with accumulation of E4orf4 in the ... Adenovirus E4orf4 protein reduces phosphorylation of c-Fos and E1A proteins while simultaneously reducing the level of AP-1. J ... Adenovirus E4orf4 protein reduces phosphorylation of c-Fos and E1A proteins while simultaneously reducing the level of AP-1. J ... Adenovirus E4orf4 protein binds to protein phosphatase 2A, and the complex down regulates E1A-enhanced junB transcription. J. ...
E1A) gene is regulated by ligation to the 3′ untranslated region (UTR) of PTGS2 (also known as COX2), the gene encoding ... The expression of various proteins associated with rapid responses to inflammation and/or proliferation can be controlled at ... Genetic targeting of an adenovirus vector via replacement of the fiber protein with the phage T4 fibritin. J. Virol. 75, 4176- ... Figure 5: AD-E1A COX is oncolytic to high-P-MAPK tumors.. ... Figure 4: Replication of Ad-E1A-COX correlates with P-MAPK ...
Human cyclin A is adenovirus E1A-associated protein p60 and behaves differently from cyclin B. ... The requirements for protein synthesis and degredation, and the control of destruction of cyclins A and B in the meiotic and ... The clam embryo protein cyclin A induces entry into M phase and the resumption of meiosis in Xenopus oocytes. ... Cyclin: a protein specified by maternal mRNA in sea urchin eggs that is destroyed at each cleavage division. ...
RNA binding proteins represent more than 15% of the total amount of proteins [113]. In several cases studied to date, ... Minimal RB-responsive E1A promoter modification to attain potency, selectivity, and transgene-arming capacity in oncolytic ... adenoviruses. Mol. Ther 2010, 18, 1960-1971. [Google Scholar]. *Castello, A.; Horos, R.; Strein, C.; Fischer, B.; Eichelbaum, K ... interaction between proteins and RNAs results in conformational changes to the protein, the RNA or both, which could endow the ...
Repressor Proteins. 1. + 427. Nucleoside-Diphosphate Kinase. 1. + 428. Adenovirus E1A Proteins. 1. + ...
Adenovirus E1A Proteins. *Algorithms. *Alleles. *Alpha-Amanitin. *Amino Acid Sequence. *Animals. *Bacterial Proteins ...
EWS protein/E1A enhancer binding protein chimera; adenovirus E1A enhancer-binding protein; ets variant gene 4 (E1A enhancer- ... ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with the C-terminal FLAG-6 His tags.. Such ...
These primary cell cultures were often noted to spontaneously degenerate over time, and adenoviruses are now known to be a ... Adenovirus, a DNA virus, was first isolated in the 1950s in adenoid tissue-derived cell cultures, hence the name. ... Thereafter, adenovirus produces potent E1A proteins that immortalize primary rodent cells by altering cellular transcription, ... Adenovirus became a significant cause of economic cost and morbidity in this setting. A live oral vaccine against adenovirus ...
Direct interaction between adenovirus E1A protein and the TATA box binding transcription factor IID. ... Adenovirus E1A activation domain binds the basic repeat in the TATA box transcription factor. ... Purification of his-tagged proteins in non-denaturing conditions suggests a convenient method for protein interaction studies. ... Weighing naked proteins: practical, high-accuracy mass measurement of peptides and proteins ...
The protein is differentially regulated by E1A-induced phosphorylation. The full-length gene product represses transcription ... one of several cellular transcription factors whose DNA-binding activities are regulated through the action of adenovirus E1A. ... A 50-kDa amino-terminal product is generated from the full-length protein through proteolytic cleavage. ... The zinc finger protein encoded by this gene is ... from the E4 promoter in the absence of E1A, while the 50-kDa ...
E1A-PLECs and E1A-NLFs positive for E1A DNA, mRNA and protein expressed cytokeratin and vimentin but not smooth muscle α-actin ... E1A-PLECs and E1A-NLFs positive for E1A DNA, mRNA and protein expressed cytokeratin and vimentin but not smooth muscle α-actin ... E1A-PLECs and E1A-NLFs positive for E1A DNA, mRNA and protein expressed cytokeratin and vimentin but not smooth muscle α-actin ... E1A-PLECs and E1A-NLFs positive for E1A DNA, mRNA and protein expressed cytokeratin and vimentin but not smooth muscle α-actin ...
  • Treatment of adenovirus-infected mouse S49 cells with cAMP analogs leads to the transcriptional induction of early viral genes. (princeton.edu)
  • E1A proteins and cAMP work in synergy to activate several of these genes. (princeton.edu)
  • The AP-1 activity binds efficiently to both AP-1 and activating transcription factor (ATF)/cAMP response element binding protein (CREB)-binding sites present in E1A-inducible promoters and presumably plays a role in the transcriptional activation of adenovirus genes by E1A proteins and cAMP. (princeton.edu)
  • Induction of integrated adenovirus E1A and E1B genes in transformed human cells by phorbol ester tumor promoters. (microbiologyresearch.org)
  • These data provide the first evidence that Rb and Rb-related proteins can directly regulate DNA replication and that components of licensing factor are targets of the products of tumor suppressor genes. (xenbase.org)
  • The proteins encoded by Myb genes bind to DNA and regulate the expression of other genes that control cell division, differentiation, and cell death. (stanford.edu)
  • Identified as a cellular target of the adenoviral oncoprotein E1A, it is required for both transcriptional activation and repression of viral genes. (nih.gov)
  • Also involved in transcription activation mediated by the adenovirus E1A, a viral oncoprotein that deregulates transcription of key genes. (icr.ac.uk)
  • Since HCMV can complement the development of the adenovirus E1A mutant (45), we had been interested in identifying whether HCMV an infection may possibly also alter the appearance from the IFN-/-governed immediate-early response genes. (thetechnoant.info)
  • To exploit this mechanism, the researchers developed two adenoviruses, AdARET and AdAREF, that contained the AREs of human TNF- a and c-fos genes in the 3'-untranslated regions of the E1A gene. (health.news)
  • Similarly, combinations of adenovirus E1A + E1B or human papillomavirus E6 + E7 genes are able to replace T-antigen functions and permit cell proliferation, whereas the individual genes do not. (elsevier.com)
  • It is not possible to determine whether heat-shock response is essential for virus replication, because the implicated viral genes (such as Ad5 E1A, ref. 10) also control other essential replication steps. (elsevier.com)
  • Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS . (nih.gov)
  • Controls the onset and duration of the resting stage through genes whose transcriptional activity is controlled by its p53 protein. (wikilectures.eu)
  • A panel of group B adenoviruses expressing individual mouse adenovirus 1 genes were also unable to rescue EnAd replication. (ox.ac.uk)
  • 15 ], who have proved the capacity of the tumor-specific promoter hTERT to drive the expression of the apoptin and E1A genes in prostate carcinoma cells and in mouse models. (hindawi.com)
  • In U937V cells IFN alpha induced the formation of the complex between early-region-2 transcription factor 1 (E2F-1) and retinoblastoma protein (RB) which is known to repress transcription of E2F-1-inducible genes, necessary for cell cycle progression. (iegt-rostock.de)
  • The improved amount of these proteins prospects to a decreased amount of pAKt1 and improved quantity of non-phosphorylated FOXO3a, which migrates in the cytoplasm towards the nucleus and escalates the transcription of its proapoptotic focus on genes. (racetab.org)
  • The cell line was later confirmed to have incorporated the Ad5 E1A and E1B genes into chromosome 19, E1 region being necessary for activation of viral promoters and expression of both early and late genes. (synabs.be)
  • Experimental Design: We constructed an adenovirus 5 vector [tumor- or telomerase-specific replication-competent adenovirus (TRAD)], in which the hTERT promoter element drives expression of E1A and E1B genes linked with an internal ribosome entry site, and we examined the selective replication and antitumor effect in human cancer cells in vitro and in vivo. (elsevier.com)
  • The finding helps explain how E1A generates and manages its functional complexity-a complexity that for viral proteins seems particularly necessary, considering how tiny viral genomes are in comparison to those of their animal hosts. (scripps.edu)
  • Of interest is the role of adenoviruses as viral vectors in vaccination and in gene therapy.For example, in viral vector vaccines against SARS-CoV2, the vector virus is used to deliver RNA encoding SARS-CoV2 spike protein into target cells. (medscape.com)
  • Accelerated onset of viral transcription in adenovirus-infected HeLa cells treated with the tumor promoter 12- O - tetradecanoyl-phorbol-13-acetate. (microbiologyresearch.org)
  • E1A is thus able to deactivate a critical component of the host defense against viral infection. (elsevier.com)
  • Like members of the coronavirus family, adenoviruses commonly infect the lungs and airways (besides other organs) and cause viral pneumonia. (health.news)
  • Such responses might be host reactions to the synthesis of foreign protein, or might be irrelevant consequences of the viral need to activate transcription. (elsevier.com)
  • Alternatively, as heat-shock proteins can facilitate protein folding, activating a heat-shock response might be a specific virus function ensuring proper synthesis of viral proteins and virions. (elsevier.com)
  • The human being embryonic kidney (HEK) 293 cell range and its own derivatives are found in experiments which range from sign transduction and proteins interaction research over viral product packaging to fast small-scale protein manifestation and biopharmaceutical creation. (alexjordan.org)
  • Graham achieved to transfect HK cells (subline of the ubiquitous keratin-forming tumor cell line HeLa) with adenovirus type 5 DNA, thanks to the forming of a calcium phosphate-DNA precipitate, a technique still in use today in many viral vector companies. (synabs.be)
  • 1. Lung transplantation requirements for viral replication.8 the deleted e1 region adenovirus vectors are epichromosomal, therapeutic gene is ret). (aestheticscienceinstitute.edu)
  • The study focused on an IDP known as the adenovirus "early region 1A oncoprotein" (E1A). (scripps.edu)
  • p400 is a cellular protein which binds to the adenovirus E1A oncoprotein(s). (elsevier.com)
  • Postdoctoral fellows Allan Chris M. Ferreon and Josephine C. Ferreon, in the Deniz and Wright laboratories, respectively, used the single-molecule FRET method to detail the strengths ("affinities") with which E1A binds to two of its most important protein partners. (scripps.edu)
  • First, like many folded proteins, E1A turns out to employ a basic regulatory mechanism called allostery: when one protein partner binds at one part of the E1A structure, it changes the ability of the other major binding site on E1A to bind other partners. (scripps.edu)
  • This gene encodes a protein that binds to the C-terminus of adenovirus E1A proteins. (prosci-inc.com)
  • The amino-terminal region of the retinoblastoma gene product binds a novel nuclear matrix protein that co-localizes to centers for RNA processing. (xenbase.org)
  • Yeast TATA-binding protein TFIID binds to TATA elements with both consensus and nonconsensus DNA sequences. (wikidata.org)
  • It binds to cyclins A and E as well as binds to and may be involved in the transforming capacity of the adenovirus E1A protein. (rockland.com)
  • It is known that E1A also binds to p300 and other host-cellular transcriptional factors, and is thought to be involved in transcriptional regulation by formation of the complex with these factors. (jnkinhibitor.com)
  • Adenoviral E1A function through Myc. (semanticscholar.org)
  • Adenoviral control regions activated by E1a and cAMP response element bind to the same factor. (microbiologyresearch.org)
  • We describe an adenoviral vector that is conditionally replication competent in which expression of the essential adenoviral early region 1A (E1A) gene is regulated by ligation to the 3′ untranslated region (UTR) of PTGS2 (also known as COX2 ), the gene encoding prostaglandin-endoperoxide synthase 2, allowing activated RAS/P-MAPK-specific stabilization of its mRNA. (nature.com)
  • Adenoviral transduction efficiency was assessed by fluorescent staining of cancer cell lines using adenovirus type 5 containing the green fluorescent protein gene (Ad5GFP). (biomedcentral.com)
  • E1a mRNA expression in the treated tumors and expression of the replication-specific adenoviral hexon protein were evaluated. (biomedcentral.com)
  • Deletion of the RB1 gene , which occurs during the development of hereditary retinoblastoma, or sequestration of its protein product in the presence of adenoviral E1A protein or E7 protein (in human papilloma virus infection) induces unblocking of E2F suppression. (wikilectures.eu)
  • A 4-kbp adenoviral genome fragment may possess integrated in chromosome 19 (ref. 4) and encodes for the E1A/E1B protein, which hinder the cell routine control counteract and pathways apoptosis5,6. (alexjordan.org)
  • The biological function of p400 is not yet known, but it is structurally and immunologically closely related to p300 and CREB-binding protein, two known E1A-binding transcription adapters. (elsevier.com)
  • CREB (cAMP responsive element binding protein) binding protein (CBP) and adenovirus E1A-associated 300 kDa protein (p300) are histone acetyltransferases, which are necessary for multiple cellular processes. (edpsciences.org)
  • CREB (cAMP responsive element binding protein) binding protein (CBP) and p300 are lysine acetyl transferases that play a key role as transcriptional coactivators in cells [ 1 - 6 ]. (edpsciences.org)
  • CBP and p300 are large proteins with ∼2400 amino acids and share ~75% sequence similarity ( Figure 1 ) [ 11 , 17 , 18 ]. (edpsciences.org)
  • CBP and p300 consist of several highly conserved domains, including a catalytic histone acetyltransferase (HAT) domain that acetylates histones and other proteins, and an adjacent bromodomain that recognizes acetylated histone tails [ 19 ]. (edpsciences.org)
  • IRF3, which includes previously been proven to obtain no intrinsic transcriptional activation potential, interacts using the transcriptional coactivator CREB binding proteins, however, not with p300, to create CIF. (thetechnoant.info)
  • Tat transcriptional activity can be reliant on lysine acetylation mediated by nuclear histone acetyltransferases p300/CBP (E1A binding proteins p300/CREB binding proteins) and PCAF (P300/CBP-associated aspect). (racetab.org)
  • Both SRCAP and p400 are known to be p300/CBP binding proteins and previous reports have demonstrated that p300/CBP is important for activation of the Notch signaling pathway. (jnkinhibitor.com)
  • Amazingly, we discovered that HCMV an infection per se led to a sturdy transcriptional activation from the ISRE-controlled ISG54 gene and that activation happened in the lack of de novo proteins synthesis. (thetechnoant.info)
  • Biochemical characterization shows that it is made up of a lately described participant from the interferon regulatory aspect (IRF) family members and the transcriptional coactivator CREB binding proteins (CBP). (thetechnoant.info)
  • Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. (stjohnslabs.com)
  • A strategy for constructing replication-defective adenovirus vectors from non-subgroup C viruses has been successfully demonstrated with adenovirus type 7 strain a (Ad7a) as the prototype. (cornell.edu)
  • Development of a sensitive non-radioactive protein kinase assay and its application for detecting DYRK activity in Xenopus laevis oocytes. (xenbase.org)
  • The nature of protein kinase c activation by physically defined phospholipid vesicle and diacylgly-cerols. (microbiologyresearch.org)
  • Protein kinase C contains a pseudosubstrate prototope in its regulatory domain. (microbiologyresearch.org)
  • The GLI zinc finger transcription factors act at the end of the HH signaling cascade to control gene expression, and recent studies have shown that the activity of GLI proteins can be additionally modified by integration of distinct signals, such as the MEK/extracellular signal-regulated kinase (ERK) and phosphinositide-3 kinase (PI3K)/AKT pathway. (aacrjournals.org)
  • Interacts with CTNNB1, GLI3 and MAML1.SIMILARITY Belongs to the protein kinase superfamily. (reactome.org)
  • CMGC Ser/Thr protein kinase family. (reactome.org)
  • The induction of pRB phosphorylation correlates with E1A-mediated induction of p34cdc2 expression and kinase activity, consistent with the possibility that p34cdc2 is a pRB kinase. (cshl.edu)
  • E1A domain 2 mutants do not bind detectable levels of pRB but remain competent to induce pRB phosphorylation and to activate cdc2 protein kinase expression and activity. (cshl.edu)
  • p16, Cyclin Dependent Kinase Inhibitor 2A (CDK2NA), generates several transcripts/proteins which differ from each other in their first exons. (rockland.com)
  • Cell surface expression of glycosylated, nonglycosylated, and truncated forms of a cytoplasmic protein pyruvate kinase. (vanderbilt.edu)
  • Conversely, infection with adenovirus encoding dominant-negative (DN) Nrf2 (ad-DN-Nrf2) or pretreatment with the selective phosphatidylinositol-3 kinase inhibitor LY294002 inhibited the tBHQ-mediated promoter response and corresponding neuroprotection. (jneurosci.org)
  • Elevated appearance of PTEN decreases serine/threonine proteins kinase pAkt1 amounts, which cause decreased phosphorylation of FOXO3a. (racetab.org)
  • Prostate attenuated replication competent adenovirus (ARCA) CN706: a selective cytotoxic for prostate-specific antigen-positive prostate cancer cells. (nature.com)
  • We investigated the antitumor effect of the hTERT-specific replication-competent adenovirus on human cancer cells. (elsevier.com)
  • Virogenic products such as E1A (adenovirus infection), T121 antigen (from SV virus) or E7 (from human papillomavirus) bind to Rb and then have a similar effect. (wikilectures.eu)
  • Gene products such as E1b (from adenovirus), large antigen from SV-virus an d E-6 antigen from papillomavirus, which bind to the p53 protein, contribute to this. (wikilectures.eu)
  • Type 5 of Adenovirus infects mainly epithelial cells and causes a mild pathology with flulike symptoms and is mainly used to study the molecular biology of adenoviruses. (semanticscholar.org)
  • The first is lytic infection, which occurs when an adenovirus enters human epithelial cells and continues through an entire replication cycle, which results in cytolysis, cytokine production, and induction of host inflammatory response. (medscape.com)
  • Guinea pig primary peripheral lung epithelial cells (PLECs) transfected with adenovirus E1A (E1A-PLECs) were compared to guinea pig normal lung fibroblasts (NLFs) transfected with E1A (E1A-NLFs). (edu.sa)
  • E1A transfection results in the transformation of peripheral lung epithelial cells and normal lung fibroblasts to a phenotype intermediate between that of the two primary cells. (edu.sa)
  • The genome of adenovirus is well known and can be modified with relative ease to induce lysis or cytotoxicity of a specified cell line without affecting others. (medscape.com)
  • Cytoplasmic levels of c-fos and junB mRNAs are rapidly increased by cAMP, and the induction is substantially stronger in the presence of E1A protein. (princeton.edu)
  • In transformed cells, induction of apoptosis by adenovirus type 2 (Ad2) early region 4 ORF 4 (E4orf4) correlates with accumulation of E4orf4 in the cell membrane-cytoskeleton fraction. (rupress.org)
  • Figure 2: Induction of Hras stabilizes E1A-COX2 expression. (nature.com)
  • These results imply that direct physical interaction between the RB and E1A products does not play a required role in the early stages of E1A-mediated cell cycle induction and that pRB phosphorylation is not, of itself, sufficient to allow quiescent cells to divide. (cshl.edu)
  • As we found no differences between these cell lines concerning the induction by IFN alpha of the pathway involving tyrosine-protein kinases and the signal transducer and activator of transcription (Jak-Stat), we examined whether cell-cycle-regulating proteins are differently affected by IFN alpha in U937VR and U937VR cells. (iegt-rostock.de)
  • The induction of phosphatase and tensin homolog (PTEN) and FOXO3a was seen in cells that exhibit just the Tat proteins, recommending that Tat may be an integral player in the activation of the pathways. (racetab.org)
  • It is concluded that the level of E1A protein is a critical determinant of oncolytic phenotype and a completely novel strategy for the design and construction of conditionally replicative adenoviruses is proposed. (semanticscholar.org)
  • Midkine promoter-based conditionally replicative adenovirus might be a promising new gene therapy for pancreatic cancer. (biomedcentral.com)
  • We show that the adenovirus E1A protein inhibits NO production by decreasing expression of the inducible NO synthase (NOS2). (elsevier.com)
  • This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein. (rockland.com)
  • During adenovirus an infection, it would appear that the proteins encoded with the E1A gene inhibits the DNA-binding capability of ISGF3, leading to the transcriptional suppression from the mobile ISGs (17, 18, 42). (thetechnoant.info)
  • ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with the C-terminal FLAG-6 His tags. (novoprolabs.com)
  • This review will highlight the major vector platforms that are currently in development (including adenoviruses, reoviruses, vaccinia viruses, herpesviruses, and coxsackieviruses) and how they are combined with checkpoint inhibitors. (springeropen.com)
  • Construction of an adenovirus type 7a E1A- vector. (cornell.edu)
  • Ad7a-CAT represents the first example of a non-subgroup C E1A- adenovirus gene transfer vector. (cornell.edu)
  • To boost the basic safety of systemic anti-p21Ras scFv delivery for therapy lately and metastatic stage malignancies, in this scholarly study, we utilized CIK cells as another vector to transport the recombinant adenovirus KGHV500 that harbored the anti-p21Ras scFv gene to tumor foci, and investigated its anti-colorectal cancers results then. (ourworldisblue.com)
  • Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. (reactome.org)
  • In KGHV400 the E1B and E1A promoters were replaced and controlled with the hTERT and HRE promoters. (ourworldisblue.com)
  • These primary cell cultures were often noted to spontaneously degenerate over time, and adenoviruses are now known to be a common cause of asymptomatic respiratory tract infection that produces in vitro cytolysis in these tissues. (medscape.com)
  • Adenovirus is often cultured from the pharynx and stool of asymptomatic children, and most adults have measurable titers of anti-adenovirus antibodies, implying prior infection. (medscape.com)
  • Recovery from adenovirus infection is associated with the development of serotype-specific neutralizing antibodies. (medscape.com)
  • Upon infection with adenovirus, one of three different interactions with the cells may occur. (medscape.com)
  • The present report concerns the role of adenovirus infection in inducing this transformation in an animal model of chronic obstructive pulmonary disease. (edu.sa)
  • Microbial infection) Antiviral activity is antagonized by oncoproteins, such as papillomavirus (HPV) protein E7 and adenovirus early E1A protein (PubMed:26405230). (cansar.ai)
  • Expression of human CD46 and trans-complementation by murine adenovirus 1 fails to allow productive infection by a group B oncolytic adenovirus in murine cancer cells. (ox.ac.uk)
  • Unlike previous reports on group C adenoviruses, trans-complementation of group B adenoviruses by co-infection with mouse adenovirus 1 did not rescue replication. (ox.ac.uk)
  • By reintroducing Nrf2 via infection with a replication-deficient adenovirus (ad), both the genetic response and neuroprotection were rescued. (jneurosci.org)
  • CMVElB plasmid and adenovirus ElB monoclonal antibodies, E. White. (elsevier.com)
  • Here, we aim to develop a murine cell line capable of supporting replication of a group B oncolytic adenovirus, enadenotucirev (EnAd), for incorporation into a syngeneic immunocompetent animal model to explore the oncolytic vaccine potential of group B oncolytic viruses. (ox.ac.uk)
  • Siders, W.M., Halloran, P.J. & Fenton, R.G. Transcriptional targeting of recombinant adenoviruses to human and murine melanoma cells. (nature.com)
  • In this study, we investigated the effects of IL-12p40 as a genetic adjuvant on immune modulation using recombinant adenoviruses expressing IL-12p40 (rAd/IL-12p40) and OVA (rAd/OVA). (aai.org)
  • Adenovirus has been associated with both sporadic and epidemic disease and, with regard to infections among military recruits, who were routinely immunized against types 4 and 7 from 1971 until the cessation of vaccine production in 1996. (medscape.com)
  • Findings point to an important role for p400 in Myc function and reveal that E1A drives oncogenesis by tapping into two important transcriptional networks: those of E2F and Myc. (semanticscholar.org)
  • To develop a novel therapeutic strategy for human pancreatic cancer using a midkine promoter-based conditionally replicating adenovirus. (biomedcentral.com)
  • Full-length Rb and MCM7 form protein complexes in vitro, and the amino termini of two Rb-related proteins, p107 and p130 , also bind MCM7 . (xenbase.org)
  • Protein complexes between Rb and MCM7 were also detected in anti-Rb immunoprecipitates prepared from human cells. (xenbase.org)
  • Large T antigens of many polyomaviruses are able to form complexes with the retinoblastoma protein. (xenbase.org)
  • Both exhibited cuboidal morphology and junctional complexes, but did not contain lamellar bodies or express surfactant protein A, B or C mRNAs. (edu.sa)
  • Adapter protein, which is found in various multiprotein chromatin complexes with histone acetyltransferase activity (HAT), which gives a specific tag for epigenetic transcription activation. (icr.ac.uk)
  • Probably acts by linking transcription factors such as E1A, MYC or E2F1 to HAT complexes such as STAGA thereby allowing transcription activation. (icr.ac.uk)
  • The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. (rockland.com)
  • Formation of the early-region-2 transcription-factor-1-retinoblastoma-protein (E2F-1-RB) transrepressor and release of the retinoblastoma protein from nuclear complexes containing cyclin A is induced by interferon in U937V cells but not in interferon-resistant U937VR cells. (iegt-rostock.de)
  • Surprisingly, the 243-amino-acid form of adenovirus-5 E1A was found subsequently to reverse-transform many human tumour cells. (semanticscholar.org)
  • An extremely hardy virus, adenovirus is ubiquitous in human and animal populations, survives long periods outside a host, and is endemic throughout the year. (medscape.com)
  • Tumor promoter alters the temporal program of adenovirus replication in human cells. (microbiologyresearch.org)
  • A yeast two-hybrid screen was employed to identify human proteins that specifically bind the amino-terminal 400 amino acids of the retinoblastoma (Rb) protein. (xenbase.org)
  • Sequence of cDNA comprising the human pur gene and sequence-specific single-stranded-DNA-binding properties of the encoded protein. (xenbase.org)
  • Physical interaction of the retinoblastoma protein with human D cyclins. (xenbase.org)
  • An adenovirus mutant that replicates selectively in p53-deficient human tumor cells. (nature.com)
  • SV40 also expresses the oncogenic protein T Ag in human mesotheliomas, which inactivates p53 and RB. (sv40foundation.org)
  • We now demonstrate using a syngeneic mouse tumor model, expressing an Ag derived from the early region 1A of human adenovirus type 5, that the inadequate nature of the antitumor CTL response is not due to direct Ag presentation by the tumor cells, but results from presentation of tumor-derived Ag by nonactivated CD11c(+) APC. (cnrs.fr)
  • Higashino and his team then tested the two modified adenoviruses on cultured cells and in nude mice injected with human cancer cells. (health.news)
  • We examined midkine mRNA expression and midkine protein expression by seven human pancreatic cancer cell lines (AsPC-1, BxPC-3, CFPAC-1, HPAC, MIAPaCa-2, PANC-1, and Suit-2), as well as by non-cancerous pancreatic tissue and pancreatic cancers. (biomedcentral.com)
  • Murine cells can be readily modified to express surface human CD46, one of the receptors for group B adenoviruses, allowing receptor-mediated uptake of EnAd particles into the murine cells and expression of CMV promoter-driven transgenes. (ox.ac.uk)
  • Although the early E1A mRNA was expressed in murine cells at levels similar to human cells, adenovirus E2B and Fibre mRNA expression levels were hampered and few virus genomes were produced. (ox.ac.uk)
  • Apoptin, a protein derived from chicken anemia virus VP3 gene, is able to induce selective apoptosis in human tumor and transformed cells but shows little or no cytotoxic effect in many normal human cells [ 16 , 17 ]. (hindawi.com)
  • The initial 293 cells1,2,3 had been produced in 1973 through the kidney of the aborted human being embryo of unfamiliar parenthood by change with sheared Adenovirus 5 DNA. (alexjordan.org)
  • it is able to perform human post-translational modifications on secreted recombinant proteins. (synabs.be)
  • Results: TRAD induced selective E1A and E1B expression in human cancer cells, but not in normal cells such as human fibroblasts. (elsevier.com)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Complement Factor H Related Protein 5 (CFHR5) in serum, plasma and other biological fluids. (operatiebrp.nl)
  • Description: Quantitativesandwich ELISA kit for measuring Human Complement factor H-related protein 5 (CFHR5) in samples from serum, plasma, tissue homogenates. (operatiebrp.nl)
  • Description: A competitive ELISA for quantitative measurement of Human Complement factor H related protein 5(CFHR5) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (operatiebrp.nl)
  • Activation of transcription factor IIIC by adenovirus E1A protein. (microbiologyresearch.org)
  • EGFR/MEK/ERK signaling induces JUN/activator protein 1 activation, which is essential for oncogenic transformation, in combination with the GLI activator forms GLI1 and GLI2. (aacrjournals.org)
  • In an attempt to reveal some of the cell type-specific changes resulting from ARE activation, cultures were infected with adenovirus encoding green fluorescent protein (GFP) (ad-GFP) or ad-DN-Nrf2 (containing GFP) before tBHQ treatment. (jneurosci.org)
  • Both midkine mRNA expression and midkine protein expression were strong in AsPC-1 and CFPAC-1 cell liens, moderate in BxPC-3, HPAC, and Suit-2 cell lines, and weak in PANC-1 and MIAPaCa-2 cell lines. (biomedcentral.com)
  • The C-terminal region of E1A: a molecular tool for cellular cartography. (semanticscholar.org)
  • What is known of the ways E1A interferes with growth regulation by these and other cellular proteins, such as cyclins and transcription factors, so as to bring about oncogenic transformation is described. (semanticscholar.org)
  • E1A proteins interact with a variety of key cellular molecules to quickly subvert the cell's replication machinery for the benefit of the virus. (scripps.edu)
  • Transcriptional repression is critical for genetic regulation of many cellular proteins, and involves either a transcription regulator that has an intrinsic repressor domain, or a transcription regulator that contains a binding site in the regulatory domain where a co-repressor can bind. (bdbiosciences.com)
  • The zinc finger protein encoded by this gene is one of several cellular transcription factors whose DNA-binding activities are regulated through the action of adenovirus E1A. (nih.gov)
  • We present evidence for the possible involvement of both the RB and p53 proteins in the regulation of cellular senescence. (elsevier.com)
  • Cellular heat-shock responses, which are characterized as elevation and relocalization of heat-shock proteins, occur during replication of many viruses. (elsevier.com)
  • The adenovirus early region 1A (E1A) proteins were described originally as immortalizing oncoproteins that altered transcription in rodent cells. (semanticscholar.org)
  • Tumour suppression apparently results from the ability of E1A to re-programme transcription in tumour cells, and the molecular basis of this intriguing effect is now beginning to emerge. (semanticscholar.org)
  • Adenovirus E1A proteins are closely associated with chromatin in productively infected and transformed cells. (semanticscholar.org)
  • This review focuses on how the C-terminus of E1A affects cell cycle progression, apoptosis, senescence, transformation, and conversion of cells to an epithelial state through interactions with CTBP1/2, DYRK1A/B, FOXK1/ 2, and importin-α. (semanticscholar.org)
  • Results demonstrate that the E1A gene products can act negatively to suppress the transformed phenotype in neu-transformed cells. (semanticscholar.org)
  • We now demonstrate that the transcription factor AP-1 is modestly induced by cAMP in S49 cells and induced to significantly higher levels by cAMP in the presence of E1A proteins. (princeton.edu)
  • Such proteins frequently play outsized roles in cells, as crucial "molecular hubs" within very large protein-interaction networks. (scripps.edu)
  • We have studied the initial effects of adenovirus E1A expression on the retinoblastoma (RB) gene product in normal quiescent cells. (cshl.edu)
  • Although binding of the E1A products to pRB could, in theory, make pRB phosphorylation unnecessary for cell cycle progression, we have found that the 12S wild-type E1A product is capable of inducing phosphorylation of pRB in normal quiescent cells. (cshl.edu)
  • These results suggest that the E1A products do not need to bind pRB in order to stimulate resting cells to enter the cell cycle. (cshl.edu)
  • Indeed, a more important role of the RB binding activity of the E1A products may be to prevent dividing cells from returning to G0. (cshl.edu)
  • We directly addressed the impact of immature vs mature DC on CTL responses by infusing adenovirus peptide-loaded DC (of the D1 cell line) into mice that had received adenovirus-specific naive TCR-transgenic CD8+ T cells. (cnrs.fr)
  • Interestingly, adoptive transfer of CTL effector cells harvested after priming by either mature or immature DC into naive recipient mice, followed by exposure to adenovirus, yielded quantitatively and qualitatively indistinguishable CTL memory responses. (cnrs.fr)
  • In normal cells, the production of proteins is regulated by adenylate-uridylate-rich elements (AREs), which are RNA elements that enhance the rapid degradation of mRNAs. (health.news)
  • They found that in cancer cells, the expression of the E1A protein was higher than in normal cells, and virus production, as well as cytolytic (cell lysis) activity, was also higher. (health.news)
  • The idea behind the insertion is that the AREs will stabilize the killer adenoviruses, allowing them to replicate only inside cancer cells but not in normal healthy ones," explained Fumihiro Higashino, a molecular oncologist at Hokkaido University and one of the authors of the study. (health.news)
  • CONCLUSION: Together, these results indicate that there may be major differences in the early stages of replication of group C and B adenoviruses in murine cells, and that the block to the life cycle of B adenoviruses in murine cells occurs in the early stage of virus replication, perhaps reflecting poor activity of Ad11p E1A in murine cells. (ox.ac.uk)
  • Provided the Xanthiside wide usage of 293 cells for biomedical pathogen/proteins and study creation, we made a decision Xanthiside to perform a thorough genomic characterization from the 293 cell range as well as the most commonly utilized produced lines (Fig. 1a) to raised understand the dynamics from the 293 genome beneath the methods commonly found in biotechnological executive of mammalian cell lines. (alexjordan.org)
  • HIV Tat is one of the proteins that can contribute to the apoptotic process of both infected and uninfected cells, as it is definitely released in the plasma and enter uninfected cells. (racetab.org)
  • Conclusions Our research provides a book strategy for the treating colorectal cancers by merging CIK cells using the recombinant adenovirus KGHV500 which transported anti-p21 Ras scFv. (ourworldisblue.com)
  • Further study confirmed a recombinant adenovirus having the gene for anti-p21Ras scFv could penetrate tumor cells, express anti-p21Rseeing that scFv and inhibit the proliferation of tumor cells with p21Rseeing that overexpression intracellularly. (ourworldisblue.com)
  • HEK293 cells and SW480 cells had been harvested in the 1640 moderate supplemented with 10% heat-inactivated fetal bovine serum Eslicarbazepine (FBS) (Biological Sectors, Israel,#64C001-1ACS) under atmospheric circumstances of 5% CO2 at 37?C. Recombinant adenovirus Recombinant adenovirus KGHV400 was built previously by us predicated on a wild-type adenovirus (Advertisement5). (ourworldisblue.com)
  • Galvanized by his success with HK cells, Frank is confident that he will succeed in transfecting HEK cells with type 5 adenoviruses. (synabs.be)
  • All IFNs identified in vertebrates are secreted proteins, with IFN-α being secreted from virus-infected white blood cells. (biomedcentral.com)
  • Adenovirus is known to be oncogenic in rodents but not in humans. (medscape.com)
  • HPV is present in cervical cancer, where it expresses the oncogenic proteins E6 and E7 that inactivate the host regulatory proteins p53 and RB, respectively. (sv40foundation.org)
  • The full-length gene product represses transcription from the E4 promoter in the absence of E1A, while the 50-kDa form acts as a transcriptional activator in its presence. (nih.gov)
  • Replication of adenovirus type 5 containing the 0.6 kb midkne promoter (Ad5MK) was assessed by the detection of E1 protein in cancer cell lines. (biomedcentral.com)
  • An E1A-deleted Ad7a reporter virus expressing the chloramphenicol acetyltransferase (CAT) gene from the cytomegalovirus promoter enhancer was constructed with DNA fragments isolated from Ad7a, an Ad7a recombination reporter plasmid, and the 293 cell line. (cornell.edu)
  • Schizosaccharomyces pombe cdc4+ gene encodes a novel EF-hand protein essential for cytokinesis. (vanderbilt.edu)
  • The tumor suppressor gene TP53 , which encodes the p53 protein, is a key regulatory factor that monitors DNA damage. (wikilectures.eu)
  • A purified adenovirus 289-amino-acid E1A protein activates RNA polymerase III transcription in vitro and alters TFIIIC. (microbiologyresearch.org)
  • Enhancement of RNA polymerase III transcription by E1A gene product of adenovirus. (microbiologyresearch.org)
  • Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. (reactome.org)
  • Reengineering adenovirus regulatory pathways to enhance oncolytic specificity and efficacy. (nature.com)
  • The switch from early to late transcription in phage GA-1: characterization of the regulatory protein p4G. (araid.es)
  • This is a particular challenge for group B adenoviruses, which fail to infect even those immunocompetent animal model systems identified as semi-permissive for type 5 adenovirus. (ox.ac.uk)
  • The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit. (xenbase.org)
  • Lately, anti-p21Ras scFv was made by all of us that could react with mutant p21Ras and wild-type p21Ras proteins [10]. (ourworldisblue.com)
  • And this thought obsesses him all day long in his laboratory: to leave its mark on adenovirus-type viruses' research. (synabs.be)
  • The type III interferons are also known as the IFN-λs and are more related to type I IFNs based on their amino acid sequence and protein function. (biomedcentral.com)
  • The protein is differentially regulated by E1A-induced phosphorylation. (nih.gov)
  • An adenovirus starts producing copies of E1A shortly after it infects a cell. (scripps.edu)
  • HH signaling is initiated by the binding of HH protein to its receptor Patched (PTCH), a transmembrane domain protein that represses signal transduction in the absence of ligand by inhibiting the transmembrane domain protein Smoothened (SMO). (aacrjournals.org)
  • Adenovirus, a DNA virus, was first isolated in the 1950s in adenoid tissue-derived cell cultures, hence the name. (medscape.com)
  • The retinoblastoma protein is phosphorylated during specific phases of the cell cycle. (xenbase.org)
  • To determine the relative contribution of nuclear E4orf4 versus membrane-associated E4orf4 to cell death signaling, we engineered green fluorescent fusion proteins to target E4orf4 to specific cell compartments. (rupress.org)
  • p130, Retinoblastoma-like protein 2 (Rb2), is a key regulator of entry into cell division. (rockland.com)
  • In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are capable of inducing cell cycle arrest in G1 and G2 phases. (rockland.com)
  • 1994. Expression of thyrotropin-releasing hormone receptors by adenovirus-mediated gene transfer reveals that thyrotropin-releasing hormone desensitization is cell specific. . (cornell.edu)
  • E1A expression was higher in cell lines with strong midkine expression than in cell lines with weak midkine expression. (biomedcentral.com)
  • Disruption of cell cycle control results in the absence of both alleles of a particular suppressor gene (e.g., deletions), alterations in their structure (e.g., point mutations), or inactivation of the protein encoded by them. (wikilectures.eu)
  • We have analysed the different regulation of cell-cycle-relevant proteins by interferon alpha (IFN alpha) in IFN alpha-sensitive and resistant U937 leukemic cell lines. (iegt-rostock.de)
  • Negative regulation of DNA replication by the retinoblastoma protein is mediated by its association with MCM7 . (xenbase.org)
  • The first discovered tumor suppressor gene was called the retinoblastoma gene (RB1 gene) and its product RB-protein (pRB ). (wikilectures.eu)
  • For gene therapy, the SSAT gene E2F-1 and [11] gene [12] carried by adenovirus exhibit significant antitumor activity against CRC in vitro. (ourworldisblue.com)
  • Cao, W, Bao, C & Lowenstein, CJ 2003, ' Inducible nitric oxide synthase expression inhibition by adenovirus E1A ', Proceedings of the National Academy of Sciences of the United States of America , vol. 100, no. 13, pp. 7773-7778. (elsevier.com)
  • Intratumoral injection from the recombinant adenovirus demonstrated intracellular appearance of anti-p21Ras scFv and apparent inhibition of transplanted tumor development. (ourworldisblue.com)
  • IDPs also include proteins that are linked to major diseases, including the tumor suppressor protein p53, the alpha synuclein protein of Parkinson's disease, and the amyloid beta and tau proteins of Alzheimer's disease. (scripps.edu)
  • The same complex also interacts with proteins of the Rb tumor suppressor family and the E2F transcription factor family. (stanford.edu)
  • Stabilization of the p53 tumor suppressor is induced by adenovirus 5 E1A and accompanies apoptosis. (vanderbilt.edu)
  • The prevention of cancer is profoundly dependent on the p53 tumor suppressor protein. (biologists.com)
  • An element of symmetry in yeast TATA-box binding protein transcription factor IID. (wikidata.org)
  • Two independent cDNAs resulting from this screen were found to encode the carboxy-terminal 137 amino acids of MCM7 , a member of a family of proteins that comprise replication licensing factor. (xenbase.org)
  • Downmodulation of E1A protein expression as a novel strategy to design cancer-selective adenoviruses. (semanticscholar.org)
  • The amino-terminal portion of E1A decreases transactivation of the NOS2 5′-flanking region, limiting the DNA binding activity of NF-κB and inhibiting NOS2 expression. (elsevier.com)
  • Both proteins can also interact with a polycomb group protein complex which participates in regulation of gene expression during development. (prosci-inc.com)
  • The expression of various proteins associated with rapid responses to inflammation and/or proliferation can be controlled at the level of mRNA stability. (nature.com)
  • Here we report that expression of Gam1, a protein encoded by the avian virus CELO (ref. 11), elevates and relocalizes hsp70 and hsp40. (elsevier.com)
  • In the Suit-2 subcutaneous xenograft model, expression of E1A was detected in Ad5MK-treated tumors, but not in untreated and Ad5GFP-treated tumors. (biomedcentral.com)
  • E1A is worth studying not just because it facilitates adenovirus infections, but also because it's a prime example of an IDP. (scripps.edu)
  • e20718 HCV NS3 Can Activate Notch-Signaling Pathway tumorigenesis of adenovirus infections. (jnkinhibitor.com)