Proteins transcribed from the E1A genome region of ADENOVIRUSES which are involved in positive regulation of transcription of the early genes of host infection.
Species of the genus MASTADENOVIRUS, causing a wide range of diseases in humans. Infections are mostly asymptomatic, but can be associated with diseases of the respiratory, ocular, and gastrointestinal systems. Serotypes (named with Arabic numbers) have been grouped into species designated Human adenovirus A-F.
Proteins transcribed from the E4 region of ADENOVIRUSES. The E4 19K protein transactivates transcription of the adenovirus E2F protein and complexes with it.
Proteins transcribed from the E1B region of ADENOVIRUSES which are involved in regulation of the levels of early and late viral gene expression.
Proteins encoded by adenoviruses that are synthesized prior to, and in the absence of, viral DNA replication. The proteins are involved in both positive and negative regulation of expression in viral and cellular genes, and also affect the stability of viral mRNA. Some are also involved in oncogenic transformation.
Proteins transcribed from the E3 region of ADENOVIRUSES but not essential for viral replication. The E3 19K protein mediates adenovirus persistence by reducing the expression of class I major histocompatibility complex antigens on the surface of infected cells.
A family of non-enveloped viruses infecting mammals (MASTADENOVIRUS) and birds (AVIADENOVIRUS) or both (ATADENOVIRUS). Infections may be asymptomatic or result in a variety of diseases.
Respiratory and conjunctival infections caused by 33 identified serotypes of human adenoviruses.
Proteins transcribed from the E2 region of ADENOVIRUSES. Several of these are required for viral DNA replication.
Virus diseases caused by the ADENOVIRIDAE.
The very first viral gene products synthesized after cells are infected with adenovirus. The E1 region of the genome has been divided into two major transcriptional units, E1A and E1B, each expressing proteins of the same name (ADENOVIRUS E1A PROTEINS and ADENOVIRUS E1B PROTEINS).
Products of viral oncogenes, most commonly retroviral oncogenes. They usually have transforming and often protein kinase activities.
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
Established cell cultures that have the potential to propagate indefinitely.
A genus of ADENOVIRIDAE that infects MAMMALS including humans and causes a wide range of diseases. The type species is Human adenovirus C (see ADENOVIRUSES, HUMAN).
Species of the genus MASTADENOVIRUS that causes fever, edema, vomiting, and diarrhea in dogs and encephalitis in foxes. Epizootics have also been caused in bears, wolves, coyotes, and skunks. The official species name is Canine adenovirus and it contains two serotypes.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
The functional hereditary units of VIRUSES.
Deoxyribonucleic acid that makes up the genetic material of viruses.
An inheritable change in cells manifested by changes in cell division and growth and alterations in cell surface properties. It is induced by infection with a transforming virus.
A member of the p300-CBP transcription factors that was originally identified as a binding partner for ADENOVIRUS E1A PROTEINS.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Any of the processes by which cytoplasmic factors influence the differential control of gene action in viruses.
Product of the retinoblastoma tumor suppressor gene. It is a nuclear phosphoprotein hypothesized to normally act as an inhibitor of cell proliferation. Rb protein is absent in retinoblastoma cell lines. It also has been shown to form complexes with the adenovirus E1A protein, the SV40 T antigen, and the human papilloma virus E7 protein.
Those proteins recognized by antibodies from serum of animals bearing tumors induced by viruses; these proteins are presumably coded for by the nucleic acids of the same viruses that caused the neoplastic transformation.
The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.
A transcription factor that possesses DNA-binding and E2F-binding domains but lacks a transcriptional activation domain. It is a binding partner for E2F TRANSCRIPTION FACTORS and enhances the DNA binding and transactivation function of the DP-E2F complex.
Species of the genus MASTADENOVIRUS, causing neurological disease in pigs.
Proteins found in any species of virus.
A genus of ADENOVIRIDAE that infects birds. The type species is FOWL ADENOVIRUS A.
A ubiquitously expressed regulatory protein that contains a retinoblastoma protein binding domain and an AT-rich interactive domain. The protein may play a role in recruiting HISTONE DEACETYLASES to the site of RETINOBLASTOMA PROTEIN-containing transcriptional repressor complexes.
Techniques and strategies which include the use of coding sequences and other conventional or radical means to transform or modify cells for the purpose of treating or reversing disease conditions.
Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.
A family of basic helix-loop-helix transcription factors that control expression of a variety of GENES involved in CELL CYCLE regulation. E2F transcription factors typically form heterodimeric complexes with TRANSCRIPTION FACTOR DP1 or transcription factor DP2, and they have N-terminal DNA binding and dimerization domains. E2F transcription factors can act as mediators of transcriptional repression or transcriptional activation.
The type species of the genus AVIADENOVIRUS, family ADENOVIRIDAE, an oncogenic virus of birds. This is also called CELO virus for chick embryo lethal orphan virus.
The introduction of functional (usually cloned) GENES into cells. A variety of techniques and naturally occurring processes are used for the gene transfer such as cell hybridization, LIPOSOMES or microcell-mediated gene transfer, ELECTROPORATION, chromosome-mediated gene transfer, TRANSFECTION, and GENETIC TRANSDUCTION. Gene transfer may result in genetically transformed cells and individual organisms.
Processes that stimulate the GENETIC TRANSCRIPTION of a gene or set of genes.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Proteins that form the CAPSID of VIRUSES.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
A species of POLYOMAVIRUS originally isolated from Rhesus monkey kidney tissue. It produces malignancy in human and newborn hamster kidney cell cultures.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Eukaryotic cell line obtained in a quiescent or stationary phase which undergoes conversion to a state of unregulated growth in culture, resembling an in vitro tumor. It occurs spontaneously or through interaction with viruses, oncogenes, radiation, or drugs/chemicals.
Specific molecular components of the cell capable of recognizing and interacting with a virus, and which, after binding it, are capable of generating some signal that initiates the chain of events leading to the biological response.
Nuclear phosphoprotein encoded by the p53 gene (GENES, P53) whose normal function is to control CELL PROLIFERATION and APOPTOSIS. A mutant or absent p53 protein has been found in LEUKEMIA; OSTEOSARCOMA; LUNG CANCER; and COLORECTAL CANCER.
Use of attenuated VIRUSES as ANTINEOPLASTIC AGENTS to selectively kill CANCER cells.
Activating transcription factors were originally identified as DNA-BINDING PROTEINS that interact with early promoters from ADENOVIRUSES. They are a family of basic leucine zipper transcription factors that bind to the consensus site TGACGTCA of the cyclic AMP response element, and are closely related to CYCLIC AMP-RESPONSIVE DNA-BINDING PROTEIN.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Polyomavirus antigens which cause infection and cellular transformation. The large T antigen is necessary for the initiation of viral DNA synthesis, repression of transcription of the early region and is responsible in conjunction with the middle T antigen for the transformation of primary cells. Small T antigen is necessary for the completion of the productive infection cycle.
Tumor-selective, replication competent VIRUSES that have antineoplastic effects. This is achieved by producing cytotoxicity-enhancing proteins and/or eliciting an antitumor immune response. They are genetically engineered so that they can replicate in CANCER cells but not in normal cells, and are used in ONCOLYTIC VIROTHERAPY.
Simultaneous inflammation of the cornea and conjunctiva.
The transfer of bacterial DNA by phages from an infected bacterium to another bacterium. This also refers to the transfer of genes into eukaryotic cells by viruses. This naturally occurring process is routinely employed as a GENE TRANSFER TECHNIQUE.
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
A negative regulator of the CELL CYCLE that undergoes PHOSPHORYLATION by CYCLIN-DEPENDENT KINASES. It contains a conserved pocket region that binds E2F4 TRANSCRIPTION FACTOR and interacts with viral ONCOPROTEINS such as POLYOMAVIRUS TUMOR ANTIGENS; ADENOVIRUS E1A PROTEINS; and PAPILLOMAVIRUS E7 PROTEINS.
A member of the p300-CBP transcription factor family that was initially identified as a binding partner for CAMP RESPONSE ELEMENT-BINDING PROTEIN. Mutations in CREB-binding protein are associated with RUBINSTEIN-TAYBI SYNDROME.
Cell changes manifested by escape from control mechanisms, increased growth potential, alterations in the cell surface, karyotypic abnormalities, morphological and biochemical deviations from the norm, and other attributes conferring the ability to invade, metastasize, and kill.
An enzyme that catalyzes the acetylation of chloramphenicol to yield chloramphenicol 3-acetate. Since chloramphenicol 3-acetate does not bind to bacterial ribosomes and is not an inhibitor of peptidyltransferase, the enzyme is responsible for the naturally occurring chloramphenicol resistance in bacteria. The enzyme, for which variants are known, is found in both gram-negative and gram-positive bacteria. EC 2.3.1.28.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
The process by which a DNA molecule is duplicated.
ONCOGENE PROTEINS from papillomavirus that deregulate the CELL CYCLE of infected cells and lead to NEOPLASTIC CELL TRANSFORMATION. Papillomavirus E7 proteins have been shown to interact with various regulators of the cell cycle including RETINOBLASTOMA PROTEIN and certain cyclin-dependent kinase inhibitors.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
An E2F transcription factor that interacts directly with RETINOBLASTOMA PROTEIN and CYCLIN A and activates GENETIC TRANSCRIPTION required for CELL CYCLE entry and DNA synthesis. E2F1 is involved in DNA REPAIR and APOPTOSIS.
Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release.
Ribonucleic acid that makes up the genetic material of viruses.
Genes whose gain-of-function alterations lead to NEOPLASTIC CELL TRANSFORMATION. They include, for example, genes for activators or stimulators of CELL PROLIFERATION such as growth factors, growth factor receptors, protein kinases, signal transducers, nuclear phosphoproteins, and transcription factors. A prefix of "v-" before oncogene symbols indicates oncogenes captured and transmitted by RETROVIRUSES; the prefix "c-" before the gene symbol of an oncogene indicates it is the cellular homolog (PROTO-ONCOGENES) of a v-oncogene.
This line KB is now known to be a subline of the ubiquitous KERATIN-forming tumor cell line HeLa. It was originally thought to be derived from an epidermal carcinoma of the mouth, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via contamination by HELA CELLS. The cells are positive for keratin by immunoperoxidase staining. KB cells have been reported to contain human papillomavirus18 (HPV-18) sequences.
Inflammation, often mild, of the conjunctiva caused by a variety of viral agents. Conjunctival involvement may be part of a systemic infection.
Proteins that control the CELL DIVISION CYCLE. This family of proteins includes a wide variety of classes, including CYCLIN-DEPENDENT KINASES, mitogen-activated kinases, CYCLINS, and PHOSPHOPROTEIN PHOSPHATASES as well as their putative substrates such as chromatin-associated proteins, CYTOSKELETAL PROTEINS, and TRANSCRIPTION FACTORS.
Cis-acting DNA sequences which can increase transcription of genes. Enhancers can usually function in either orientation and at various distances from a promoter.
Transport proteins that carry specific substances in the blood or across cell membranes.
Vaccines used to prevent infection by any virus from the family ADENOVIRIDAE.
A conserved A-T rich sequence which is contained in promoters for RNA polymerase II. The segment is seven base pairs long and the nucleotides most commonly found are TATAAAA.
A sequence of successive nucleotide triplets that are read as CODONS specifying AMINO ACIDS and begin with an INITIATOR CODON and end with a stop codon (CODON, TERMINATOR).
A genus of the family PARVOVIRIDAE, subfamily PARVOVIRINAE, which are dependent on a coinfection with helper adenoviruses or herpesviruses for their efficient replication. The type species is Adeno-associated virus 2.
The outer protein protective shell of a virus, which protects the viral nucleic acid.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Proteins prepared by recombinant DNA technology.
Serologic tests in which a positive reaction manifested by visible CHEMICAL PRECIPITATION occurs when a soluble ANTIGEN reacts with its precipitins, i.e., ANTIBODIES that can form a precipitate.
The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.
A phosphoprotein phosphatase subtype that is comprised of a catalytic subunit and two different regulatory subunits. At least two genes encode isoforms of the protein phosphatase catalytic subunit, while several isoforms of regulatory subunits exist due to the presence of multiple genes and the alternative splicing of their mRNAs. Protein phosphatase 2 acts on a broad variety of cellular proteins and may play a role as a regulator of intracellular signaling processes.
Enzymes catalyzing the transfer of an acetyl group, usually from acetyl coenzyme A, to another compound. EC 2.3.1.
Genes that are introduced into an organism using GENE TRANSFER TECHNIQUES.
A general transcription factor that plays a major role in the activation of eukaryotic genes transcribed by RNA POLYMERASES. It binds specifically to the TATA BOX promoter element, which lies close to the position of transcription initiation in RNA transcribed by RNA POLYMERASE II. Although considered a principal component of TRANSCRIPTION FACTOR TFIID it also takes part in general transcription factor complexes involved in RNA POLYMERASE I and RNA POLYMERASE III transcription.
Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected.
The major sequence-specific DNA-binding component involved in the activation of transcription of RNA POLYMERASE II. It was originally described as a complex of TATA-BOX BINDING PROTEIN and TATA-BINDING PROTEIN ASSOCIATED FACTORS. It is now know that TATA BOX BINDING PROTEIN-LIKE PROTEINS may take the place of TATA-box binding protein in the complex.
Visible morphologic changes in cells infected with viruses. It includes shutdown of cellular RNA and protein synthesis, cell fusion, release of lysosomal enzymes, changes in cell membrane permeability, diffuse changes in intracellular structures, presence of viral inclusion bodies, and chromosomal aberrations. It excludes malignant transformation, which is CELL TRANSFORMATION, VIRAL. Viral cytopathogenic effects provide a valuable method for identifying and classifying the infecting viruses.
Products of proto-oncogenes. Normally they do not have oncogenic or transforming properties, but are involved in the regulation or differentiation of cell growth. They often have protein kinase activity.
A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.
Viruses which enable defective viruses to replicate or to form a protein coat by complementing the missing gene function of the defective (satellite) virus. Helper and satellite may be of the same or different genus.
A cell line derived from cultured tumor cells.
Tumor suppressor genes located on human chromosome 13 in the region 13q14 and coding for a family of phosphoproteins with molecular weights ranging from 104 kDa to 115 kDa. One copy of the wild-type Rb gene is necessary for normal retinal development. Loss or inactivation of both alleles at this locus results in retinoblastoma.
Head to tail array of covalently joined DNA sequences generated by concatenation. Concatenated DNA is attached end to end in contrast to CATENATED DNA which is attached loop to loop.
Viruses which lack a complete genome so that they cannot completely replicate or cannot form a protein coat. Some are host-dependent defectives, meaning they can replicate only in cell systems which provide the particular genetic function which they lack. Others, called SATELLITE VIRUSES, are able to replicate only when their genetic defect is complemented by a helper virus.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
Mutant mice homozygous for the recessive gene "nude" which fail to develop a thymus. They are useful in tumor studies and studies on immune responses.
DNA viruses producing malignant tumors. Of the six major groupings of DNA viruses four contain members which are actually or potentially oncogenic: the Adenoviridae, the Herpesviridae, the Papovaviridae, and the Poxviridae.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
A sequence of amino acids in a polypeptide or of nucleotides in DNA or RNA that is similar across multiple species. A known set of conserved sequences is represented by a CONSENSUS SEQUENCE. AMINO ACID MOTIFS are often composed of conserved sequences.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
The sum of the weight of all the atoms in a molecule.
A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.
A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.
Genes whose expression is easily detectable and therefore used to study promoter activity at many positions in a target genome. In recombinant DNA technology, these genes may be attached to a promoter region of interest.
Biochemical identification of mutational changes in a nucleotide sequence.
Actual loss of portion of a chromosome.
Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). Lines have been developed using several different strains of mice. Tissues are usually fibroblasts derived from mouse embryos but other types and sources have been developed as well. The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION.
A negative regulator of the CELL CYCLE that undergoes PHOSPHORYLATION by CYCLIN-DEPENDENT KINASES. RBL2 contains a conserved pocket region that binds E2F4 TRANSCRIPTION FACTOR and E2F5 TRANSCRIPTION FACTOR. RBL2 also interacts with viral ONCOPROTEINS such as POLYOMAVIRUS TUMOR ANTIGENS; ADENOVIRUS E1A PROTEINS; and PAPILLOMAVIRUS E7 PROTEINS.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
A genus of the family PICORNAVIRIDAE whose members preferentially inhabit the intestinal tract of a variety of hosts. The genus contains many species. Newly described members of human enteroviruses are assigned continuous numbers with the species designated "human enterovirus".
Structures that are part of or contained in the CELL NUCLEUS.
A family of histone acetyltransferases that is structurally-related to CREB-BINDING PROTEIN and to E1A-ASSOCIATED P300 PROTEIN. They function as transcriptional coactivators by bridging between DNA-binding TRANSCRIPTION FACTORS and the basal transcription machinery. They also modify transcription factors and CHROMATIN through ACETYLATION.
A subclass of enzymes which includes all dehydrogenases acting on primary and secondary alcohols as well as hemiacetals. They are further classified according to the acceptor which can be NAD+ or NADP+ (subclass 1.1.1), cytochrome (1.1.2), oxygen (1.1.3), quinone (1.1.5), or another acceptor (1.1.99).
An E2F transcription factor that interacts directly with RETINOBLASTOMA PROTEIN and CYCLIN A. E2F2 activates GENETIC TRANSCRIPTION required for CELL CYCLE entry and DNA synthesis.
A large family of regulatory proteins that function as accessory subunits to a variety of CYCLIN-DEPENDENT KINASES. They generally function as ENZYME ACTIVATORS that drive the CELL CYCLE through transitions between phases. A subset of cyclins may also function as transcriptional regulators.
Sequences of DNA or RNA that occur in multiple copies. There are several types: INTERSPERSED REPETITIVE SEQUENCES are copies of transposable elements (DNA TRANSPOSABLE ELEMENTS or RETROELEMENTS) dispersed throughout the genome. TERMINAL REPEAT SEQUENCES flank both ends of another sequence, for example, the long terminal repeats (LTRs) on RETROVIRUSES. Variations may be direct repeats, those occurring in the same direction, or inverted repeats, those opposite to each other in direction. TANDEM REPEAT SEQUENCES are copies which lie adjacent to each other, direct or inverted (INVERTED REPEAT SEQUENCES).
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.
Cellular DNA-binding proteins encoded by the c-jun genes (GENES, JUN). They are involved in growth-related transcriptional control. There appear to be three distinct functions: dimerization (with c-fos), DNA-binding, and transcriptional activation. Oncogenic transformation can take place by constitutive expression of c-jun.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
A genus of ADENOVIRIDAE that comprises viruses of several species of MAMMALS and BIRDS. The type species is Ovine adenovirus D.
Synthetic or natural oligonucleotides used in hybridization studies in order to identify and study specific nucleic acid fragments, e.g., DNA segments near or within a specific gene locus or gene. The probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the probe include the radioisotope labels 32P and 125I and the chemical label biotin.
Enzymes that oxidize certain LUMINESCENT AGENTS to emit light (PHYSICAL LUMINESCENCE). The luciferases from different organisms have evolved differently so have different structures and substrates.
A ubiquitously expressed zinc finger-containing protein that acts both as a repressor and activator of transcription. It interacts with key regulatory proteins such as TATA-BINDING PROTEIN; TFIIB; and ADENOVIRUS E1A PROTEINS.
Process of growing viruses in live animals, plants, or cultured cells.
A family of small, non-enveloped DNA viruses infecting birds and most mammals, especially humans. They are grouped into multiple genera, but the viruses are highly host-species specific and tissue-restricted. They are commonly divided into hundreds of papillomavirus "types", each with specific gene function and gene control regions, despite sequence homology. Human papillomaviruses are found in the genera ALPHAPAPILLOMAVIRUS; BETAPAPILLOMAVIRUS; GAMMAPAPILLOMAVIRUS; and MUPAPILLOMAVIRUS.
Tumor suppressor genes located on the short arm of human chromosome 17 and coding for the phosphoprotein p53.
A DNA-dependent RNA polymerase present in bacterial, plant, and animal cells. It functions in the nucleoplasmic structure where it transcribes DNA into RNA. It has specific requirements for cations and salt and has shown an intermediate sensitivity to alpha-amanitin in comparison to RNA polymerase I and II. EC 2.7.7.6.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
Proteins that are normally involved in holding cellular growth in check. Deficiencies or abnormalities in these proteins may lead to unregulated cell growth and tumor development.
Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.
The complete genetic complement contained in a DNA or RNA molecule in a virus.
A species of CERCOPITHECUS containing three subspecies: C. tantalus, C. pygerythrus, and C. sabeus. They are found in the forests and savannah of Africa. The African green monkey (C. pygerythrus) is the natural host of SIMIAN IMMUNODEFICIENCY VIRUS and is used in AIDS research.
A genus of potentially oncogenic viruses of the family POLYOMAVIRIDAE. These viruses are normally present in their natural hosts as latent infections. The virus is oncogenic in hosts different from the species of origin.
Cellular DNA-binding proteins encoded by the c-myc genes. They are normally involved in nucleic acid metabolism and in mediating the cellular response to growth factors. Elevated and deregulated (constitutive) expression of c-myc proteins can cause tumorigenesis.
The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990)
Enzymes that catalyze acyl group transfer from ACETYL-CoA to HISTONES forming CoA and acetyl-histones.
Nuclear antigen with a role in DNA synthesis, DNA repair, and cell cycle progression. PCNA is required for the coordinated synthesis of both leading and lagging strands at the replication fork during DNA replication. PCNA expression correlates with the proliferation activity of several malignant and non-malignant cell types.
The rate dynamics in chemical or physical systems.
The ultimate exclusion of nonsense sequences or intervening sequences (introns) before the final RNA transcript is sent to the cytoplasm.
A diverse class of enzymes that interact with UBIQUITIN-CONJUGATING ENZYMES and ubiquitination-specific protein substrates. Each member of this enzyme group has its own distinct specificity for a substrate and ubiquitin-conjugating enzyme. Ubiquitin-protein ligases exist as both monomeric proteins multiprotein complexes.
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
A family of transcription factors found primarily in PLANTS that bind to the G-box DNA sequence CACGTG or to a consensus sequence CANNTG.
Invasion of the host RESPIRATORY SYSTEM by microorganisms, usually leading to pathological processes or diseases.
Production of new arrangements of DNA by various mechanisms such as assortment and segregation, CROSSING OVER; GENE CONVERSION; GENETIC TRANSFORMATION; GENETIC CONJUGATION; GENETIC TRANSDUCTION; or mixed infection of viruses.
A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.
Immunoglobulins produced in response to VIRAL ANTIGENS.
Proteins coded by oncogenes. They include proteins resulting from the fusion of an oncogene and another gene (ONCOGENE PROTEINS, FUSION).
A protein that has been shown to function as a calcium-regulated transcription factor as well as a substrate for depolarization-activated CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASES. This protein functions to integrate both calcium and cAMP signals.
Minute infectious agents whose genomes are composed of DNA or RNA, but not both. They are characterized by a lack of independent metabolism and the inability to replicate outside living host cells.
Process of determining and distinguishing species of bacteria or viruses based on antigens they share.
An area showing altered staining behavior in the nucleus or cytoplasm of a virus-infected cell. Some inclusion bodies represent "virus factories" in which viral nucleic acid or protein is being synthesized; others are merely artifacts of fixation and staining. One example, Negri bodies, are found in the cytoplasm or processes of nerve cells in animals that have died from rabies.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Process of generating a genetic MUTATION. It may occur spontaneously or be induced by MUTAGENS.
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
Inflammation of the lung parenchyma that is caused by a viral infection.
A suborder of PRIMATES consisting of six families: CEBIDAE (some New World monkeys), ATELIDAE (some New World monkeys), CERCOPITHECIDAE (Old World monkeys), HYLOBATIDAE (gibbons and siamangs), CALLITRICHINAE (marmosets and tamarins), and HOMINIDAE (humans and great apes).
Elements of limited time intervals, contributing to particular results or situations.
Substances elaborated by viruses that have antigenic activity.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
A DNA-dependent RNA polymerase present in bacterial, plant, and animal cells. It functions in the nucleoplasmic structure and transcribes DNA into RNA. It has different requirements for cations and salt than RNA polymerase I and is strongly inhibited by alpha-amanitin. EC 2.7.7.6.
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
Membrane proteins encoded by the BCL-2 GENES and serving as potent inhibitors of cell death by APOPTOSIS. The proteins are found on mitochondrial, microsomal, and NUCLEAR MEMBRANE sites within many cell types. Overexpression of bcl-2 proteins, due to a translocation of the gene, is associated with follicular lymphoma.
A ubiquitously expressed complement receptor that binds COMPLEMENT C3B and COMPLEMENT C4B and serves as a cofactor for their inactivation. CD46 also interacts with a wide variety of pathogens and mediates immune response.
Deletion of sequences of nucleic acids from the genetic material of an individual.
Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.
A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.
The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.
Suspensions of attenuated or killed viruses administered for the prevention or treatment of infectious viral disease.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Motifs in DNA- and RNA-binding proteins whose amino acids are folded into a single structural unit around a zinc atom. In the classic zinc finger, one zinc atom is bound to two cysteines and two histidines. In between the cysteines and histidines are 12 residues which form a DNA binding fingertip. By variations in the composition of the sequences in the fingertip and the number and spacing of tandem repeats of the motif, zinc fingers can form a large number of different sequence specific binding sites.
Nucleic acid sequences involved in regulating the expression of genes.
CELL LINES derived from the CV-1 cell line by transformation with a replication origin defective mutant of SV40 VIRUS, which codes for wild type large T antigen (ANTIGENS, POLYOMAVIRUS TRANSFORMING). They are used for transfection and cloning. (The CV-1 cell line was derived from the kidney of an adult male African green monkey (CERCOPITHECUS AETHIOPS).)
INFLAMMATION of any segment of the GASTROINTESTINAL TRACT from ESOPHAGUS to RECTUM. Causes of gastroenteritis are many including genetic, infection, HYPERSENSITIVITY, drug effects, and CANCER.
The interaction of two or more substrates or ligands with the same binding site. The displacement of one by the other is used in quantitative and selective affinity measurements.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
Viruses that produce tumors.
Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.
The directional growth of an organism in response to an external stimulus such as light, touch, or gravity. Growth towards the stimulus is a positive tropism; growth away from the stimulus is a negative tropism. (From Concise Dictionary of Biology, 1990)
A genus of the family HERPESVIRIDAE, subfamily BETAHERPESVIRINAE, infecting the salivary glands, liver, spleen, lungs, eyes, and other organs, in which they produce characteristically enlarged cells with intranuclear inclusions. Infection with Cytomegalovirus is also seen as an opportunistic infection in AIDS.
Injections introduced directly into localized lesions.

Early region 1 transforming functions are dispensable for mammary tumorigenesis by human adenovirus type 9. (1/94)

Some human adenoviruses are tumorigenic in rodents. Subgroup A and B human adenoviruses generally induce sarcomas in both male and female animals, and the gene products encoded within viral early region 1 (E1 region) are both necessary and sufficient for this tumorigenicity. In contrast, subgroup D human adenovirus type 9 (Ad9) induces estrogen-dependent mammary tumors in female rats and requires the E4 region-encoded ORF1 oncoprotein for its tumorigenicity. Considering the established importance of the viral E1 region for tumorigenesis by adenoviruses, we investigated whether this viral transcription unit is also necessary for Ad9 to generate mammary tumors. The nucleotide sequence of the Ad9 E1 region indicated that the gene organization and predicted E1A and E1B polypeptides of Ad9 are closely related to those of other human adenovirus E1 regions. In addition, an Ad9 E1 region plasmid demonstrated focus-forming activity in both low-passage-number and established rat embryo fibroblasts, whereas a large deletion within either the E1A or E1B gene of this plasmid diminished transforming activity. Surprisingly, we found that introducing the same transformation-inactivating E1A and E1B deletions into Ad9 results in mutant viruses that retain the ability to elicit mammary tumors in rats. These results are novel in showing that Ad9 represents a unique oncogenic adenovirus in which the E4 region, rather than the E1 region, encodes the major oncogenic determinant in the rat.  (+info)

E1(-)E4(+) adenoviral gene transfer vectors function as a "pro-life" signal to promote survival of primary human endothelial cells. (2/94)

Although endothelial cells are quiescent and long-lived in vivo, when they are removed from blood vessels and cultured in vitro they die within days to weeks. In studies of the interaction of E1(-)E4(+) replication-deficient adenovirus (Ad) vectors and human endothelium, the cells remained quiescent and were viable for prolonged periods. Evaluation of these cultures showed that E1(-)E4(+) Ad vectors provide an "antiapoptotic" signal that, in association with an increase in the ratio of Bcl2 to Bax levels, induces the endothelial cells to enter a state of "suspended animation," remaining viable for at least 30 days, even in the absence of serum and growth factors. Although the mechanisms initiating these events are unclear, the antiapoptoic signal requires the presence of E4 genes in the vector genome, suggesting that one or more E4 open reading frames of subgroup C Ad initiate a "pro-life" program that modifies cultured endothelial cells to survive for prolonged periods.  (+info)

Generation of an adenovirus vector lacking E1, e2a, E3, and all of E4 except open reading frame 3. (3/94)

Toxicity and immunity associated with adenovirus backbone gene expression is an important hurdle to overcome for successful gene therapy. Recent efforts to improve adenovirus vectors for in vivo use have focused on the sequential deletion of essential early genes. Adenovirus vectors have been constructed with the E1 gene deleted and with this deletion in combination with an E2a, E2b, or E4 deletion. We report here a novel vector (Av4orf3nBg) lacking E1, E2a, and all of E4 except open reading frame 3 (ORF3) and expressing a beta-galactosidase reporter gene. This vector was generated by transfection of a plasmid carrying the full-length vector sequence into A30.S8 cells that express E1 and E2a but not E4. Production was subsequently performed in an E1-, E2a-, and E4-complementing cell line. We demonstrated with C57BL/6 mice that the Av4orf3nBg vector effected gene transfer with an efficiency comparable to that of the Av3nBg (wild-type E4) vector but that the former exhibited a higher level of beta-galactosidase expression. This observation suggests that E4 ORF3 alone is able to enhance RNA levels from the beta-galactosidase gene when the Rous sarcoma virus promoter is used to drive transgene expression in the mouse liver. In addition, we observed less liver toxicity in mice injected with the Av4orf3nBg vector than those injected with the Av3nBg vector at a comparable DNA copy number per cell. This study suggests that the additional deletion of E4 in an E1 and E2a deletion background may be beneficial in decreasing immunogenicity and improving safety and toxicity profiles, as well as increasing transgene capacity and expression for liver-directed gene therapy.  (+info)

Frequency and stability of chromosomal integration of adenovirus vectors. (4/94)

One of the limitations of adenovirus vectors is the lack of machinery necessary for their integration into host chromosomes, resulting in short-term gene expression in dividing cells. We analyzed frequencies of integration and persistence of gene expression from integrated adenovirus vectors. Both E1-substituted and helper-dependent adenovirus vectors achieved similar integration efficiencies of approximately 10(-3) to 10(-5) per cell, with the helper-dependent vector showing slightly higher efficiencies. In stable cell pools, gene expression of the integrated vector persisted for at least 50 cell divisions without selection. However, some stable cell clones showed changes in gene expression, which were accompanied by structural changes in the integrated vector DNA.  (+info)

Reduced toxicity, attenuated immunogenicity and efficient mediation of human p53 gene expression in vivo by an adenovirus vector with deleted E1-E3 and inactivated E4 by GAL4-TATA promoter replacement. (5/94)

A recombinant adenovirus with deleted E1 and E3, and E4-inactivated by replacing the E4 promoter with a synthetic promoter composed of a minimal TATA box and five consensus yeast GAL4-binding site elements was developed and used to express the human tumor suppresser gene p53. The toxicity and immunogenicity of this vector and vector-mediated p53 gene expression in vivo were studied in immunocompetent C3H and C57BL/6 mice. Expression of the late viral gene product, hexon protein, was observed in C3H and C57BL/6 mice injected with E4 wild-type adenovirus constructs Adv-cmv-beta-Gal (BG), Adv-cmv-hp53 (WT), and empty E1- vector Adv-E4 (EW) 3 to 28 days after injection, but was undetectable in mice treated with E4 modified empty E1- vector Adv-GAL4 (EG) or Adv-cmv-hp53-GAL4 (G4). Expression of the p53 gene was observed in both WT- and G4-injected C3H and C57BL/6 mouse livers from days 3 to 28. Ten weeks after injection, p53 gene expression was still detected in G4-treated C57BL/6 mice at similar levels, but was not detectable in WT-treated mice. Vector-induced liver toxicity was evaluated by analyzing serum transaminases (SGOT and SGPT) activities. In all cases, SGOT and SGPT activities were markedly decreased in EG-treated C3H and C57BL/6 mice compared with those in EW-treated mice on days 3, 7 and 14 after injection. In C57BL/6 mice, the total anti-adenoviral CTL activities were two- to three-fold higher in animals treated with EW vector than in those treated with EG vector. These results suggest that inactivation of the E4 promoter efficiently diminished the viral replication and the late viral gene expression, reduced host immune response and consequently reduced toxicity and prolonged the duration of transgene expression in vivo.  (+info)

Induction of endogenous genes following infection of human endothelial cells with an E1(-) E4(+) adenovirus gene transfer vector. (6/94)

Recombinant adenovirus (Ad) gene transfer vectors are effective at transferring exogenous genes to a variety of cells and tissue types both in vitro and in vivo. However, in the process of gene transfer, the Ad vectors induce the expression of target cell genes, some of which may modify the function of the target cell and/or alter the local milieu. To develop a broader understanding of Ad vector-mediated induction of endogenous gene expression, genes induced by first-generation E1(-) E4(+) Ad vectors in primary human umbilical vein endothelial cells were identified by cDNA subtraction cloning. The identified cDNAs included signaling molecules (lymphoid blast crisis [LBC], guanine nucleotide binding protein alpha type S [Galpha-S], and mitogen kinase [MEK5]), calcium-regulated/cytoskeletal proteins (calpactin p11 and p36 subunits, vinculin, and spinocerebellar ataxia [SCA1]), growth factors (insulin-like growth factor binding protein 4 and transforming growth factor beta2), glyceraldehyde-6-phosphate dehydrogenase, an expressed sequence tag, and a novel cDNA showing homology to a LIM domain sequence. Two- to sevenfold induction of the endogenous gene expression was observed at 24 h postinfection, and induction continued up to 72 h, although the timing of gene expression varied among the identified genes. In contrast to that observed in endothelial cells, the Ad vector-mediated induction of gene expression was not found following Ad vector infection of primary human dermal fibroblasts or human alveolar macrophages. Empty Ad capsids did not induce endogenous gene expression in endothelial cells. Interestingly, additional deletion of the E4 gene obviated the upregulation of genes in endothelial cells by the E1(-) E3(-) Ad vector, suggesting that genes carried by the E4 region play a central role in modifying target cell gene expression. These findings are consistent with the notion that efficient transfer of exogenous genes to endothelial cells by first-generation Ad vectors comes with the price that these vectors also induce the expression of a variety of cellular genes.  (+info)

Porcine adenovirus-3 as a helper-dependent expression vector. (7/94)

Porcine adenovirus has been proposed as a potential vector for generating novel and effective vaccines for pigs. As a prerequisite for the generation of helper-dependent porcine adenovirus-3 (PAV-3) vectors, two E1-complementing porcine cell lines expressing E1 proteins of human adenovirus-5 (HAV-5) were made. These cell lines could be efficiently transfected with DNA and allowed the rescue and propagation of a PAV-3 recombinant, PAV201, containing a 0.597 kb E3 deletion and a 0.803 kb E1A deletion. Our data demonstrate that E1A proteins of HAV-5 have the capacity to transform foetal porcine retina cells and complement for the E1A proteins of PAV-3. The green fluorescent protein (GFP) gene placed under the control of a cytomegalovirus immediate early promoter was inserted into the E1A region of the PAV201 genome. Using these cell lines, a helper-dependent PAV-3 recombinant expressing GFP, PAV202, was constructed and characterized. The wild-type PAV-3 and the recombinant PAV202 expressing GFP were used to determine the ability of the virus to enter and replicate in cells of human and animal origin under cell culture conditions. Our results suggest that PAV-3 enters but does not replicate in dog, sheep, bovine and human cells.  (+info)

Recombinant E1-deleted adenoviral vectors induce apoptosis in a rat airway epithelial mucous goblet cell line. (8/94)

Replication-defective adenoviruses (Ad) are used as vectors for delivering therapeutic genes to human airway cells. We examined whether E1-deleted Ad vectors (Ad5-CMV-LacZ) had effects on cell kinetics in SPOC1 cells, which is a rat airway epithelial mucous goblet cell line. There was a vector multiplicity of infection (moi)-dependent increase of the transduction efficiency of the LacZ reporter gene in SPOC cells. Cell proliferation was inhibited in the vector-infected cells compared with that in vehicle-exposed cells. However, increased cell death was observed in the vector-infected cells with a higher moi. The morphology of vector-exposed cells revealed apoptotic features including nuclear condensation and a fragmented nucleus. These results indicate that higher moi of vectors allows the cells to achieve higher gene transfer, but also induce apoptosis of infected cells. Minimizing the induction of apoptosis of vector-infected cells may be an important strategy for the prolongation of transduction efficiency of Ad vectors in airway epithelial mucous goblet cells.  (+info)

TY - JOUR. T1 - Effect of adenovirus gene transfer vectors on the immunologic functions of mouse dendritic cells. AU - Korst, Robert J.. AU - Mahtabifard, Ali. AU - Yamada, Reiko. AU - Crystal, Ronald. PY - 2002/1/1. Y1 - 2002/1/1. N2 - To address the effect of adenovirus (Ad) gene transfer vector transduction on the diverse functions of dendritic cells, we used an Ad vector encoding no transgene (AdNull) to transduce mouse bone-marrow-derived dendritic cells (BMDC). Initial experiments using an Ad vector encoding a marker gene (AdGFP, jellyfish green fluorescent protein) showed that the optimal ratio of infectious Ad particles to each cell was 100, when both transgene expression and resultant BMDC viability were taken into account. Exposure to AdNull resulted in upregulation of both surface activation markers (CD40, MHC class II, B7.1, B7.2, ICAM-1) and IL-12 expression by BMDC. AdNull activation of BMDC was observed in multiple strains of mice. Despite this, AdNull-transduced BMDC displayed ...
TY - JOUR. T1 - Induction of endogenous genes following infection of human endothelial cells with an E1- E4+ adenovirus gene transfer vector. AU - Ramalingam, Ramachandran. AU - Rafii, Shahin. AU - Worgall, Stefan. AU - Hackett, Neil R.. AU - Crystal, Ronald. PY - 1999/12/1. Y1 - 1999/12/1. N2 - Recombinant adenovirus (Ad) gene transfer vectors are effective at transferring exogenous genes to a variety of cells and tissue types both in vitro and in vivo. However, in the process of gene transfer, the Ad vectors induce the expression of target cell genes, some of which may modify the function of the target cell and/or alter the local milieu. To develop a broader understanding of Ad vector-mediated induction of endogenous gene expression, genes induced by first-generation E1- E4+ Ad vectors in primary human umbilical vein endothelial cells were identified by cDNA subtraction cloning. The identified cDNAs included signaling molecules (lymphoid blast crisis [LBC], guanine nucleotide binding-protein ...
Greetings- Im interested in the net surface charge on mammalian viruses and how viral charge affects viral adsorption. It is my understanding (correct me if Im wrong) that mammalian viruses have a net negative charge at physiologic pH. I find this interesting because of the net negative charge on mammalian cells due to their phospholipid content. I have been working with human adenovirus gene transfer vectors and have found that the pI of adeno capsid proteins are: Hexon = 4.5 and penton = 4.7. pI of fiber protein on adenovirus is reported to be 7.07. From these data, I have concluded that, at pH 7.0, an adeno virus particle has a negative core (Hexon and penton) with positively charged fiber protrusions. Is this correct (my chemistry is a little rusty)? If so, it would explain how negatively charged adenovirus particles might interact with negatively charged cell surfaces (via positive fiber protein). Thank you, in advance for any comments and corrections. In reading this note, if any related ...
Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.. This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details. ...
Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.. This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details. ...
The project work began with replacement of the original MCS for a completely new designed MCS containing the typical RFC10/25 pre- and suffixes. Furthermore we integrated a Strep-tag II coding gene sequence previous to the suffix. This facilitates purification and detection via Western Blot of expressed enzymes dramatically. Construction of the new MCS by four desoxyribooligonucleotides via oligonucleotide hybridization and ligation into the original pYES vector was restricted with the outermost restriction enzymes of the old MCS. Per side directed mutagenesis all forbidden restriction sides of enzymes used in RFC10/25 standard in the vector backbone were deleted. Different vector samples of this successive process are shown in the picture below. Their have been digested by NgoMIV, PstI and SpeI: The resulting fragments were decreased with each step leading to the pure vector backbone linearized by cutting in MCS. Hence our first original galactose inducible pGal1 promoter containing expression ...
localloadfile(nrngui.hoc)} {localloadfile(loadbal.hoc)} objref gidvec, cvec, splitxlist, splitixlist, cpu, splitcplx, splitindex, bal objref splitbres, splitbrlist bal = new LoadBalance() gidvec = new Vector() cvec = new Vector() splitxlist = new List() splitixlist = new List() splitbrlist = new List() cpu = new Vector() splitcplx = new Vector() splitindex = new Vector() splitbres = new Vector() proc rdat() {local i, j, k, n1, n2, n3, c, n3 localobj f, vecx, vecix, vecb f = new File() f.ropen($s1) n1 = f.scanvar() for i=0, n1-1 { n2 = f.scanvar() for j=0, n2-1 { f.scanvar() gid = f.scanvar() c = f.scanvar() n3 = f.scanvar() gidvec.append(gid) cvec.append(c) vecx = new Vector(n3) vecix = new Vector(n3) vecb = new Vector(n3) for k = 0, n3-1 { vecx.x[k] = f.scanvar() vecix.x[k] = f.scanvar() vecb.x[k] = f.scanvar() } splitxlist.append(vecx) splitixlist.append(vecix) splitbrlist.append(vecb) } } } func balance() {local i, err localobj f, si, s s =new String() sprint(s.s, %s.dat, $s2) rdat(s.s) ...
Looking for first-generation? Find out information about first-generation. Denoting electronic hardware, logical organization, and software characteristic of a first-generation computer Explanation of first-generation
Triple ligation not working - posted in Molecular Cloning: Hi all, I am trying to ligate insert 1 ( Nco1-Sac1, 2180 bp), insert2 (Sac1-Sph1, 335 bp) to a vector cut at Nco1-Sph1 sites ( 3.5 Kb). Initially, after ligation I used to get a band of around 6Kb but after transformation none of the colony screened show the presence of the insert in the plasmid DNA. I treated the vector backbone with CIP and purified . Tried to religate the inserts with CIP treated vector but no ligation was obser...
var life=require (./life.js); describe(Grid, function() { var Vector = life.Vector; var grid = new life.Grid(5, 5); it(initially undefined, function() { expect(grid.get(new Vector(1, 1))).toBe(undefined); }); it(setting a value, function() { grid.set(new Vector(1, 1), X); expect(grid.get(new Vector(1, 1))).toEqual(X); }); it(forEach, function() { var test = {grid: grid, sum: 0, method: function () { this.grid.forEach(function() { this.sum++; }, this); } }; test.grid.set(new Vector(2,3), #); test.grid.set(new Vector(3,4), #); test.method(); expect(test.sum).toBe(3); }); }); describe(BouncingCritter, function() { var bob = null; beforeEach(function () { spyOn(Math, 'random').and.returnValue(0.5); bob=new life.BouncingCritter(); }); it(constructor, function() { expect('direction' in bob).toBe(true); expect(bob.direction).toBe('s'); }); it(act, clear path, function () { var clear = {look: function () {return ;}}; ...
Box yang digunakan untuk menyimpan vaksin dalam suhu dingin untuk sementara waktu ketika akan dikirim ke tempat yang lain. Cocok untuk dokter hewan dan perlindungan botani. Volume 8 Liter dilengkapi dengan 6 ice brick. Dilengkapi dengan pengunci, handle dan thermometer suhu dalam box. Merk OneMed Spesifikasi: Type : HT
A recombinant nucleic acid used for the production of a defective adenovirus containing an inserted sequence coding for a cytokine under the control of a promoter in the genomic sequence of the recombinant adenovirus. This recombinant adenovirus is useful in the preparation of anti-tumoral drugs which can be directly injected into the tumor of the host.
var life=require (./life.js); var plan = [############################, # # # o ##, # #, # ##### #, ## # # ## #, ### ## # #, # ### # #, # #### #, # ## o #, # o # o ### #, # # #, ############################]; var Vector = life.Vector; describe(Grid, function() { var grid = new life.Grid(5, 5); it(initially undefined, function() { expect(grid.get(new life.Vector(1, 1))).toBe(undefined); }); it(setting a value, function() { grid.set(new Vector(1, 1), X); expect(grid.get(new Vector(1, 1))).toEqual(X); }); it(forEach, function() { var test = {grid: grid, sum: 0, method: function () { this.grid.forEach(function() { this.sum++; }, this); } }; test.grid.set(new Vector(2,3), #); test.grid.set(new Vector(3,4), #); test.method(); expect(test.sum).toBe(3); }); }); describe(BouncingCritter, function() { var bob = null; beforeEach(function () { spyOn(Math, 'random').and.returnValue(0.5); bob=new life.BouncingCritter(); }); it(constructor, function() { ...
if (name_declared(pkgversions) != 4 ) { execute(strdef pkgversions) } sprint(pkgversions,%sRPlot = $Revision: 1.14 $, ,pkgversions) load_file(FormatFile.hoc) load_file(ObjectClass.hoc) load_file(FileUtils.hoc) begintemplate RPlot public create_rfile, start_plot, add_graph, end_plot public run public plot public clear public set_mfrow public set_width, set_height objref xvec, yvec objref xveclist, yveclist objref rfile strdef rfilename, tmpdir objref li, lilist strdef xlab, ylab, rxlab, rylab strdef cmd objref oc strdef texlab, rlab, tmp objref sf, fu proc init() { sf = new StringFunctions() fu = new FileUtils() fu.tempdirname(tmpdir) if (numarg() == 0) { create_rfile() } if (numarg() == 1) { create_rfile($s1) } // Initialise variables and objects width=6 height=4 oc = new ObjectClass() xvec = new Vector() yvec = new Vector() li = new Vector() } proc create_rfile() { // Overide filename with argument fu.tempfilename(rfilename) if (numarg() == 1) { rfilename = $s1 } rfile = new ...
Page 1 of 3 - Creating empty vector by self-ligation - posted in Molecular Cloning: I am attempting to create an empty vector from a commercial plasmid pCMVSport-6 (4.4kb) with Prp insert (around 2.5kb). Therefore i carried out a double digestion on the pCMVSport6-Prp using Not1 and Sal1, incubate at 37 degree celcius for 2 hours. Then i run the DNA on 1% agarose gel and here i can see two band. One is the vector backbone(4.4kb) and another is the insert 2.5kb. I cut out the 4.4kb band and...
Similarity of strain- and route-dependent murine responses to an adenovirus vector using the homologous thrombopoietin cDNA as the reporter genes Academic Article ...
1. Inoculate single colonies of E. coli cells containing the promoter test construct on the vector backbone, into three 17 mm test tubes containing 5 ml of the selected pre-warmed (37°C) supplemented M9 medium with kanamycin (20 ug/ml). Note: The M9 media previously prepared will contain different carbon sources. Select the appropriate one for the carbon source you wish to test). 2. Grow the cultures in the 17 mm test tubes for approximately 20 hrs at 37°C with spinning at 70 rpm. 3. Dilute the cultures 1:100 into 5 ml of pre-warmed fresh media and grow the cultures for approximately 4 hours under the previous conditions (17 mm tubes, 37°C, spinning at 70 rpm). 4. After 4 hours, measure the OD600 of a 500 ul aliquot from each culture on a WPA Biowave Spectrophotometer. This will tell you how many cells you have. 5. Based on this OD measurement, dilute the cultures to the same OD (0.07) in 5 ml of pre-warmed fresh media and grow for one hour at 37°C. Here we try to make sure everything is at ...
Scientists have discovered a link between a prominent developmental gene and neurovascular dysfunction in Alzheimer's disease.
A complete selection of products youll need to catch, or repel mice. Traditional mouse traps, live catch mouse traps, and mouse repellents.
Encouraging efficacy data have been obtained in the hepatitis C virus (HCV) chimpanzee model using prophylactic vaccines comprising adjuvanted recombinant envelope gpE1/gpE2 glycoproteins or prime/boost immunization regimens using defective adenoviruses and plasmid DNA expressing non-structural genes. While usually not resulting in sterilizing immunity after experimental challenge, the progression to chronic, persistent infection (which is responsible for HCV-associated pathogenicity in human) is inhibited. These and other vaccine candidates are in clinical development for both prophylactic as well as possible therapeutic applications. ...
Encouraging efficacy data have been obtained in the hepatitis C virus (HCV) chimpanzee model using prophylactic vaccines comprising adjuvanted recombinant envelope gpE1/gpE2 glycoproteins or prime/boost immunization regimens using defective adenoviruses and plasmid DNA expressing non-structural genes. While usually not resulting in sterilizing immunity after experimental challenge, the progression to chronic, persistent infection (which is responsible for HCV-associated pathogenicity in human) is inhibited. These and other vaccine candidates are in clinical development for both prophylactic as well as possible therapeutic applications. ...
This research study involves two investigational drugs, an Activator Ligand (INXN-1001) in combination with an Adenovirus Vector Engineered to Express hIL-12 (INXN-2001). IL-12 is a protein that may improve the bodys natural response to disease by enhancing the ability of the immune system to kill tumor cells and may interfere with blood flow to the tumor.. The main purpose of this study is to evaluate the safety and tolerability of tumor injections of INXN-2001 given in combination with different doses of INXN-1001. ...
Are you concerned about Air Pollution in your area?. Maybe modelling air pollution will get you the answers you need for this problem.. Thats what I do full-time. Try it.. ...
objref test1 objref test2 objref results_list objref tmpvec proc record_results() { results_list = new List() tmpvec = new Vector() forall { tmpvec.record(&v(0.5)) results_list.append(tmpvec) } test1 = new Vector() test2 = new Vector() test1.record(&cen_myelin[19].v(0.5)) test2.record(&cen_myelin[0].v(0.5)) } record_results() // This saves the internal voltage of each segment in a list ...
how do i move towards the target? Vector3 target = new Vector3( _d3dd.Transform.View.M13, _d3dd.Transform.View.M23, -_d3dd.Transform.View.M33); target.Nor...
Mycoplasma hyopneumoniae causes severe economic losses to the swine industry worldwide and the prevention of its related disease, enzootic porcine pneumonia, remains a challenge. The P97 adhesin protein of M. hyopneumoniae should be a good candidate for the development of a subunit vaccine because antibodies produced against P97 could prevent the adhesion of the pathogen to the respiratory epithelial cells in vitro. In the present study, a P97 recombinant replication-defective adenovirus (rAdP97c) subunit vaccine efficiency was evaluated in pigs. The rAdP97c vaccine was found to induce both strong P97 specific humoral and cellular immune responses. The rAdP97c vaccinated pigs developed a lower amount of macroscopic lung lesions (18.5 ± 9.6%) compared to the unvaccinated and challenged animals (45.8 ± 11.5%). rAdP97c vaccine reduced significantly the severity of inflammatory response and the amount of M. hyopneumoniae in the respiratory tract. Furthermore, the average daily weight gain was ...
Hong Kong Med J 2009;15(Suppl 2):S33-6 Mouse studies of SARS coronavirus-specific immune responses to recombinant replication-defective adenovirus expressing SARS coronavirus N protein FWY Sin, SCS Ch
TY - JOUR. T1 - An effective gene-knockdown using multiple shRNA-expressing adenovirus vectors. AU - Motegi, Yukari. AU - Katayama, Kazufumi. AU - Sakurai, Fuminori. AU - Kato, Takuya. AU - Yamaguchi, Tomoko. AU - Matsui, Hayato. AU - Takahashi, Masahide. AU - Kawabata, Kenji. AU - Mizuguchi, Hiroyuki. PY - 2011/7/30. Y1 - 2011/7/30. N2 - Viral vectors expressing short hairpin RNA (shRNA) are attractive for efficient and tissue-specific RNA interference (RNAi) delivery. We and others previously reported that recombinant adenovirus (Ad) vector-mediated RNAi has great potential for a variety of applications in molecular biology studies and gene therapy. In the present study, we have developed an efficient Ad vector-mediated RNAi system, in which an Ad vector carries four shRNA-expression cassettes (Ad-multi-shRNA vector), a simple and effective strategy for enhancing the RNAi response per Ad vector particle. The data demonstrated that the Ad-multi-shRNA vectors showed an enhanced RNAi effect ...
Adenoviral Vectors for Gene treatment, moment Edition offers special, entire assurance of the gene supply cars which are in line with the adenovirus thats rising as an immense instrument in gene remedy. those fascinating new healing brokers have nice capability for the therapy of illness, making gene remedy a fast-growing box for learn. This ebook provides themes starting from the fundamental biology of adenoviruses, during the building and purification of adenoviral vectors, state-of-the-art vectorology, and using adenoviral vectors in preclinical animal types, with ultimate attention of the regulatory concerns surrounding human medical gene remedy trials. This extensive scope of knowledge offers a superb evaluate of the sector, permitting the reader to achieve an entire figuring out of the advance and use of adenoviral vectors.. ...
The information presented here serves as a brief introduction to adenovirus, recombinant adenovirus, and their advantages over alternative viral vectors.
Gene Therapy adenoviral vectors explained, information about the mechanism of Adenoviruses, Adeviral particle organisation and genome organisation of adenoviruses
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We use less quinine in our Mediterranean Tonic which, with the addition of rosemary and lemon thyme, produces a much lighter, more fragrant tonic. INGREDIENTSCarbonated Spring Water, Sugar, Acid: Citric Acid, Natural Flavours including Quinine NUTRITION/100ml36kcal9gcarb
Tagging proteins is a standard method facilitating protein detection, purification or targeting. When tagging a certain protein of interest, it is challenging to predict which tag will give optimal results and will not interfere with protein folding, activity or yields. Ideally, multiple tags and positions are tested which however complicates molecular cloning and expression vector generation. In conventional cloning, tags are either added on PCR primers (requiring a distinct primer and PCR product per tag) or provided on the vector (typically leaving a restriction site scar). Here we report a vector family of 40 plasmids allowing simple, seamless fusions of a single PCR product with various N- and C-terminal tags, signal sequences and promoters. The restriction site free cloning (RSFC) strategy presented in this paper relies on seamless cloning using type IIS restriction endonucleases. After cutting out a stuffer (placeholder) fragment from the vectors, a single PCR product can be directly inserted in
13:2; potential epub Adenovirus notions; tenure bhakti 1? On the epub Adenovirus Methods and of this interest, are above under form. 1282 An epub Adenovirus Methods and Protocols: Adenoviruses, Ad study read by Porten - Szubin 1987:187.
Addgene is a non-profit plasmid repository. Addgene facilitates the exchange of genetic material between laboratories by offering plasmids and their associated cloning data to not-for-profit laboratories around the world. Addgene provides a free online database of plasmid cloning information and references, including lists of commonly used vector backbones, popular lentiviral plasmids and molecular cloning protocols. Addgene was founded in 2004, by Melina Fan, Kenneth Fan and Benjie Chen. Addgenes headquarters are located in Cambridge, Massachusetts. Addgene accepts plasmids from researchers for distribution and archival. The organization covers the operating costs of maintaining and improving the collection by charging a nominal fee to scientists requesting plasmids. As of 2014 Addgenes repository comprised 30,000 plasmids, deposited by 1,700 labs. Its plasmid collection contains plasmids used for functions such as genome engineering (including CRISPRS), gene expression, shRNA knockdown, ...
v Parental P1 Generation = the parental generation in a breeding experiment.. v F1 generation = the first-generation offspring in a breeding experiment. (1st filial generation ...
Why Audi Q7 is better than Mercedes-Benz GLS and BMW X5. The first-generation Audi Q7 was one of the highest selling vehicles in its class around the world. Now, the comprehensively updated second-gen version is here to fill the shoes and aims to do all that and more!
Both Ad26 and Ad5 were modified to remove the E1 gene to prevent replication. Large quantities of both adenoviruses are then ... They were biotechnology-derived and contain the SARS-CoV-2 S protein cDNA. Both of them are administered into the deltoid ... Gam-COVID-Vac is a viral two-vector vaccine based on two human adenoviruses - a common cold virus - containing the gene that ... Adenovirus infections cause only mild colds in healthy individuals, but they can cause life-threatening illnesses in ...
Introduction of a viral gene that partially deregulates the cell cycle (e.g., the adenovirus type 5 E1 gene was used to ... from testing toxicity of compounds or drugs to production of eukaryotic proteins. While immortalised cell lines often originate ... Artificial expression of key proteins required for immortality, for example telomerase which prevents degradation of chromosome ...
... adenovirus e1 proteins MeSH D12.776.964.700.045.050.100 - adenovirus e1a proteins MeSH D12.776.964.700.045.050.110 - adenovirus ... adenovirus E1 proteins MeSH D12.776.624.664.520.045.050.100 - adenovirus E1A proteins MeSH D12.776.624.664.520.045.050.110 - ... adenovirus e2 proteins MeSH D12.776.624.664.520.045.070 - adenovirus e3 proteins MeSH D12.776.624.664.520.045.080 - adenovirus ... adenovirus e2 proteins MeSH D12.776.964.700.045.070 - adenovirus e3 proteins MeSH D12.776.964.700.045.080 - adenovirus e4 ...
Li Y, Graham C, Lacy S, Duncan AM, Whyte P (1994). "The adenovirus E1A-associated 130-kD protein is encoded by a member of the ... G1/S-specific cyclin-E1 is a protein that in humans is encoded by the CCNE1 gene. The protein encoded by this gene belongs to ... This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to ... Li Y, Graham C, Lacy S, Duncan AM, Whyte P (December 1993). "The adenovirus E1A-associated 130-kD protein is encoded by a ...
In Adenoviruses, it consists of two transcription units, IVa2 and IX. The late class consists primarily of structural proteins ... In Papillomaviruses, they are called E1 through E7 and also stimulate cellular replication. In Polyomavirus, the early proteins ... The late proteins make up the virus capsid. In Polyomaviruses, they are known as VP1, VP2, and VP3; in Papillomaviruses they ... The early proteins produced in Papillomaviruses and Polyomaviruses regulate the cell cycle and activate DNA replication. ...
The protein encoded by this gene is a member of the cyclin-dependent kinase family of Ser/Thr protein kinases. This protein ... Tsai LH, Harlow E, Meyerson M (September 1991). "Isolation of the human cdk2 gene that encodes the cyclin A- and adenovirus E1A ... Cyclin-dependent kinase 2 has been shown to interact with: BRCA1, CDK2AP1, CDKN1B CDKN3, CEBPA, Cyclin A1, Cyclin E1, Flap ... This increases the synthesis of histone proteins (the major protein component of chromatin), and subsequently supports the DNA ...
"Induction of endogenous genes following infection of human endothelial cells with an E1(-) E4(+) adenovirus gene transfer ... S100 calcium-binding protein A10 (S100A10), also known as p11, is a protein that is encoded by the S100A10 gene in humans and ... The S100 protein is implicated in exocytosis and endocytosis by reorganization of F-actin. The p11 protein is linked with the ... As a member of the S-100 family, its structure resembles that of the S-100A1 and S-100B proteins. This class of proteins has ...
... and relief of repression by adenovirus E1A protein". Cell. 67 (2): 377-88. doi:10.1016/0092-8674(91)90189-6. PMID 1655281. ... Park K, Atchison ML (November 1991). "Isolation of a candidate repressor/activator, NF-E1 (YY-1, delta), that binds to the ... YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein ... "Relief of YY1 transcriptional repression by adenovirus E1A is mediated by E1A-associated protein p300". Genes & Development. 9 ...
By 1900, it had been generally accepted that proteins were composed of amino acids; however, whether proteins were colloids or ... 277(31): e1-e2 Harrison, Roger G., Todd, Paul, Rudge, Scott R., Petrides D.P. Bioseparations Science and Engineering. Oxford ... Berkowitz, S.A., Philo, J.S. Monitoring the Homogeneity of Adenovirus Preparations (a Gene Therapy Delivery System) Using ... Sedimentation Velocity Analysis of Heterogeneous Protein-Protein Interactions: Lamm Equation Modeling and Sedimentation ...
... system Isoform-selective protein kinase C agonist Interaction between two proteins Nuclear export signal in a protein HEK 293 ... and could be preferentially transformed by adenovirus. Adenoviruses transform neuronal lineage cells much more efficiently than ... E1 and E3) are deleted, such as AdEasy. However, homologous recombination between the inserted cellular Ad5 sequence and the ... The cells were cultured by van der Eb; the transduction by adenovirus was performed by Frank Graham, a post-doc in van der Eb's ...
"Altered AP-1/ATF complexes in adenovirus-E1-transformed cells due to EIA-dependent induction of ATF3". Oncogene. 12 (5): 1025- ... "A human protein-protein interaction network: a resource for annotating the proteome". Cell. 122 (6): 957-68. doi:10.1016/j.cell ... ATF3+protein,+human at the US National Library of Medicine Medical Subject Headings (MeSH) FactorBook ATF3 This article ... Cyclic AMP-dependent transcription factor ATF-3 is a protein that, in humans, is encoded by the ATF3 gene. Activating ...
... adenovirus e1 proteins MeSH D23.050.327.045.060 - adenovirus e2 proteins MeSH D23.050.327.045.070 - adenovirus e3 proteins MeSH ... adenovirus e4 proteins MeSH D23.050.327.062 - antigens, viral, tumor MeSH D23.050.327.062.045 - adenovirus e1a proteins MeSH ... adenovirus e1a proteins MeSH D23.050.285.062.050 - adenovirus e1b proteins MeSH D23.050.285.062.090 - antigens, polyomavirus ... hiv core protein p24 MeSH D23.050.327.520.330 - hiv envelope protein gp41 MeSH D23.050.327.520.350 - hiv envelope protein gp120 ...
... has been shown to interact with: BRCA1, BRF1 C-Raf, Cyclin E1, Cyclin-dependent kinase 2, HDAC1, ... Li Y, Graham C, Lacy S, Duncan AM, Whyte P (Dec 1993). "The adenovirus E1A-associated 130-kD protein is encoded by a member of ... Li Y, Graham C, Lacy S, Duncan AM, Whyte P (Dec 1993). "The adenovirus E1A-associated 130-kD protein is encoded by a member of ... Retinoblastoma-like protein 2 is a protein that in humans is encoded by the RBL2 gene. ...
Sedimentation Velocity Analysis of Heterogeneous Protein-Protein Interactions: Lamm Equation Modeling and Sedimentation ... Berkowitz, S.A., Philo, J.S. Monitoring the Homogeneity of Adenovirus Preparations (a Gene Therapy Delivery System) Using ... By 1900, it had been generally accepted that proteins were composed of amino acids; however, whether proteins were colloids or ... Howlett, G.J., Minton, A.P., Rivas, G. Analytical Ultracentrifugation for the Study of Protein Association and Assembly. ...
"Identification of a cell protein (FIP-3) as a modulator of NF-kappaB activity and as a target of an adenovirus inhibitor of ... 214 (4): 538.e1-538.e7. doi:10.1016/j.ajog.2015.11.002. PMID 26571191. [1] NEMO deficiency syndrome information, Great Ormond ... IKBKG+protein,+human at the US National Library of Medicine Medical Subject Headings (MeSH) GeneReviews/NIH/NCBI/UW entry on ... Xiao G, Sun SC (October 2000). "Activation of IKKalpha and IKKbeta through their fusion with HTLV-I tax protein". Oncogene. 19 ...
... proteins and small ribonucleoproteins (snRNP). Proteins encoded by aberrantly spliced pre-mRNAs are functionally different and ... 32 (4): 757.e1-757.e11. doi:10.1016/j.neurobiolaging.2010.12.013. PMID 21295377. Rajan, P.; Elliott, DJ; Robson, CN; Leung, HY ... RNA splicing was discovered in the late 1970s through the study of adenoviruses that invade mammals and replicate inside them. ... Tau protein isoforms are created by alternative splicing of exons 2, 3 and 10. The regulation of tau splicing is specific to ...
Masuda A, Yoshikai Y, Kume H, Matsuguchi T (November 2004). "The interaction between GATA proteins and activator protein-1 ... Wang N, Verna L, Hardy S, Forsayeth J, Zhu Y, Stemerman MB (September 1999). "Adenovirus-mediated overexpression of c-Jun and c ... of c-fos and c-jun gene expressions during the vitamin-induced differentiation of mouse osteoblastic cell line MC3T3-E1 cells ... "Human cytomegalovirus IE1 protein activates AP-1 through a cellular protein kinase(s)". The Journal of General Virology. 80 ( ...
40 (9): e1-4. doi:10.1016/j.jpedsurg.2005.05.063. PMID 16150324. Kim MY, Noh JH (August 2008). "A case of Kawasaki disease with ... C-reactive protein will be elevated. Liver function tests may show evidence of hepatic inflammation and low serum albumin ... Infectious and noninfectious conditions requiring consideration include: measles and other viral infections (e.g. adenovirus, ... Urinalysis may show white blood cells and protein in the urine (pyuria and proteinuria) without evidence of bacterial growth. ...
... the protein-encoding mRNA for the transcriptional regulator Pu.1-protein, elevation of Pu.1 protein predisposes defective IgG1 ... 202 (5): 466.e1-7. doi:10.1016/j.ajog.2010.01.057. PMID 20452491. Hu YL, Fong S, Largman C, Shen WF (Sep 2010). "HOXA9 ... and adenoviruses, another virus expressing miR-155-like miRNA in chickens is the oncogenic MDV-1 whose non-oncogenic relative ... Immature B cells which are miR-155 deficient evade apoptosis as a result of elevated Bcl-2 protein levels; a protein that was ...
Encodes a protein that binds to the viral origin of replication in the long control region of the viral genome. E1 uses ATP to ... Glaunsinger BA, Lee SS, Thomas M, Banks L, Javier R (November 2000). "Interactions of the PDZ-protein MAGI-1 with adenovirus E4 ... E6 proteins also interact with the MAGUK (membrane-associated guanylate kinase family) proteins. These proteins, including MAGI ... very hydrophobic proteins that destabilise the function of many membrane proteins in the infected cell. The E5 protein of some ...
103 (9): e1. doi:10.2105/ajph.2013.301433. PMC 3780688. PMID 23865670. Friedman Kutz, Reuel M., Steven Buttram, Mance Wei, ... Notably, participants immune to the common cold virus adenovirus type 5 had a higher risk of HIV infection. The vaccine was ... or measles virus may stimulate the production of proteins suppressing HIV replication, including the β-chemokines, CD8+ cell ...
de 1999). «Regulation of cyclin A-Cdk2 by SCF component Skp1 and F-box protein Skp2». Mol. Cell. Biol. (UNITED STATES) 19 (1): ... Tsai LH, Harlow E, Meyerson M (septiembre de 1991). «Isolation of the human cdk2 gene that encodes the cyclin A- and adenovirus ... Ciclina E1[21]​[8]​[22]​[23]​[24]​[25]​. *SKP2[26]​[27]​[28]​ ... de 2000). «p12(DOC-1) is a novel cyclin-dependent kinase 2-associated protein». Mol. Cell. Biol. (UNITED STATES) 20 (17): 6300- ...
Some cells of the immune system that recognize the proteins expressed will mount an attack against these proteins and cells ... Hulst MM, Westra DF, Wensvoort G, Moormann RJ (September 1993). "Glycoprotein E1 of hog cholera virus expressed in insect cells ... Other canine vaccines include canine distemper, canine parvovirus, infectious canine hepatitis, adenovirus-2, leptospirosis, ... Egg protein is present in the influenza vaccine and yellow fever vaccine as they are prepared using chicken eggs. Other ...
Structural proteins made by the hepatitis C virus include Core protein, E1 and E2; nonstructural proteins include NS2, NS3, ... Adenovirus Adenovirus infection. RNA virus. Rotavirus. Norovirus. Astrovirus. Coronavirus. Hepatitis. DNA virus. HBV (B). RNA ... E1 serves as the fusogenic subunit and E2 acts as the receptor binding protein. E1 has 4-5 N-linked glycans and E2 has 11 N- ... E1)-E2-p7-nonstructural protein 2 (NS2)-NS3-NS4A-NS4B-NS5A-NS5B-C terminal. The mature nonstructural proteins (NS2 to NS5B) ...
The adenovirus E1B-55K protein and the hepatitis B virus HBx protein are examples of viral proteins that can perform such a ... Examples of viral Bcl-2 proteins include the Epstein-Barr virus BHRF1 protein and the adenovirus E1B 19K protein.[95] Some ... these inhibitory proteins target retinoblastoma tumor-suppressing proteins.[74] These tumor-suppressing proteins regulate the ... PROTEINS: Structure, Function, and Genetics 50, 44-48.. *^ Chen Y. L.; Li Q. Z. (2007). "Prediction of apoptosis protein ...
The adenovirus E1B-55K protein and the hepatitis B virus HBx protein are examples of viral proteins that can perform such a ... Examples of viral Bcl-2 proteins include the Epstein-Barr virus BHRF1 protein and the adenovirus E1B 19K protein.[104] Some ... these inhibitory proteins target retinoblastoma tumor-suppressing proteins.[83] These tumor-suppressing proteins regulate the ... Expression of viral proteins coupled to MHC proteins on the surface of the infected cell, allowing recognition by cells of the ...
... are dependent on acidic amino acid residues and protein-protein interaction". Nucleic Acids Research. 28 (5): 1106-13. doi: ... "Oncogene expression cloning by retroviral transduction of adenovirus E1A-immortalized rat kidney RK3E cells: transformation of ... are dependent on acidic amino acid residues and protein-protein interaction". Nucleic Acids Research. 28 (5): 1106-13. doi: ... are dependent on acidic amino acid residues and protein-protein interaction". Nucleic Acids Research. 28 (5): 1106-13. doi: ...
Furthermore, depletion of hepatic S6K1 in db/db mice with the use of an adenovirus vector encoding S6K1 shRNA resulted in down- ... SREB proteins are indirectly required for cholesterol biosynthesis and for uptake and fatty acid biosynthesis. These proteins ... proteins. However, in contrast to E-box-binding HLH proteins, an arginine residue is replaced with tyrosine making them capable ... SREBP precursors are retained in the ER membranes through a tight association with SCAP and a protein of the INSIG family. ...
negative regulation of cyclin-dependent protein serine/threonine kinase activity. • G1/S transition of mitotic cell cycle. • ... Schreiber M, Muller WJ, Singh G, Graham FL (1999). "Comparison of the effectiveness of adenovirus vectors expressing cyclin ... regulation of cyclin-dependent protein serine/threonine kinase activity. • oligodendrocyte differentiation. • negative ... Venkataramani R, Swaminathan K, Marmorstein R (1998). "Crystal structure of the CDK4/6 inhibitory protein p18INK4c provides ...
Egg protein is present in influenza and yellow fever vaccines as they are prepared using chicken eggs. Other proteins may be ... Other canine vaccines include canine distemper, canine parvovirus, infectious canine hepatitis, adenovirus-2, leptospirosis, ... "Glycoprotein E1 of hog cholera virus expressed in insect cells protects swine from hog cholera". Journal of Virology. 67 (9): ... Some cells of the immune system that recognize the proteins expressed will mount an attack against these proteins and cells ...
... these proteins are responsible for the replication of the virus.[59] E1 is a highly conserved protein in the virus, E1 is in ... Its protein makeup allows it to target four types of HPV. Gardasil contains inactive L1 proteins from four different HPV ... One such method is a vaccine based on the minor capsid protein L2, which is highly conserved across HPV genotypes.[180] Efforts ... Every subunit of the virus is composed of two proteins molecules, L1 and L2. The reason why this virus has the capability to ...
The E6/E7 proteins inactivate two tumor suppressor proteins, p53 (inactivated by E6) and pRb (inactivated by E7).[14] The viral ... Genome organization of human papillomavirus type 16, one of the subtypes known to cause cervical cancer (E1-E7 early genes, L1- ... Adenovirus Adenovirus infection. RNA virus. Rotavirus. Norovirus. Astrovirus. Coronavirus. Hepatitis. DNA virus. HBV (B). RNA ... E6 produces a protein (also called E6) that binds to and inactivates a protein in the host cell called p53. Normally, p53 acts ...
... deleted in E1 and E4, and contained human OTC cDNA. It was infused into … ... The vector used for this trial was based on human adenovirus type 5, ... Adenovirus E1 Proteins / genetics * Adenovirus E4 Proteins / genetics * Adolescent * Genetic Therapy* * Genetic Vectors / ... The vector used for this trial was based on human adenovirus type 5, deleted in E1 and E4, and contained human OTC cDNA. It was ...
1997) The adenovirus E4orf6 protein can promote E1A/E1β-induced focus formation by interfering with p53 tumor suppressor ... 1998) In vitro and in vivo biology of recombinant adenovirus vectors with E1, E1/E2a, or E1/E4 deleted. J. Virol. 72:2022-2032. ... Adenovirus vectors have been constructed with the E1 gene deleted (28, 36, 37, 45) and with E1 plus E2a, E2b, or E4 deleted (1 ... 1993) Adenovirus E4orf4 protein binds to protein phosphatase 2A, and the complex down regulates E1A-enhanced junB transcription ...
A recombinant adenovirus with deleted E1 and E3, and E4-inactivated by replacing the E4 promoter with a synthetic promoter ... Adenovirus E1 Proteins / genetics * Adenovirus E2 Proteins / genetics * Adenovirus E3 Proteins / genetics ... A recombinant adenovirus with deleted E1 and E3, and E4-inactivated by replacing the E4 promoter with a synthetic promoter ... and empty E1- vector Adv-E4 (EW) 3 to 28 days after injection, but was undetectable in mice treated with E4 modified empty E1- ...
The tools are derived from a novel tumor suppressor gene which encodes a protein referred to hereinafter as the ... Replication deficient recombinant adenovirus lacks the E1 coding sequences necessary for viral replication. This function is ... An isolated EDG1 protein or a protein which is functional equivalent said EDG1 protein, wherein said EDG1 protein comprises SEQ ... The isolated protein of claim 7. wherein said protein is a fusion protein and comprises a tag for labeling or isolating said ...
Protein Extraction and Western Blot Analysis. Nuclear proteins were extracted from HUVECs as previously described.12 Protein ... with E1- and E3-deleted adenovirus-5 genome DNA (Ψ5). An intermolecular recombination of the shuttle vector and Ψ5 then creates ... Using an adenovirus-mediated gene transfer protocol, we found that the AP-1 proteins c-Fos and c-Jun directly cause phenotypic ... By using adenovirus-mediated gene transfer, we show that overexpression of AP-1 proteins directly causes coinduction of gene ...
Wold has organized a collection of readily reproducible methods for conducting research with adenoviruses, the premier and most ... In Adenovirus Methods and Protocols, William S.M. ... Immunoprecipitation of E1 A-Containing Protein Complexes ... Simultaneous Detection of RNA and Proteins in Adenovirus-Infected Cells by Fluorescence In Situ Hybridization (FISH) and ... Methods for Creating and Analyzing Adenovirus Vectors that Express Proteins that Act on the Viral Genome ...
Upon administration of adenovirus expressing mutant HPV E6/E7, the adenovirus infects and expresses the E6 and E7 proteins. The ... A cancer vaccine composed of a replication-defective E1-deleted adenovirus vector based on chimpanzee adenovirus serotype 68 ( ... Deletion of the E1, E2b and E3 genes from Ad5 prevents anti-adenovirus immune responses. MUC1, a tumor-associated antigen (TAA ... Deletion of the E1, E2b and E3 genes from Ad5 prevents anti-adenovirus immune responses. Brachyury, a tumor-associated antigen ...
AND ANTIBODY COMPOSITIONS RELATED TO PROTEIN FRAGMENTS OF... , ... E1-MINUS ADENOVIRUSES AND USE THEREOF. The present invention is ... Genetically encoded, photocleavable proteins are derived from a fluorescent protein. Upon illumination, the proteins ... MUTANT NGAL PROTEINS AND USES THEREOF. In one aspect the present invention is directed to mutant NGAL proteins that have the ... RECOMBINANT PROTEINS HAVING FACTOR H ACTIVITY. The invention relates to a recombinant protein having factor H activity. ...
Adenovirus type 5 expresses proteins that regulate the activity and stability of the tumor suppressor p53. The adenovirus E1B- ... Replication of adenovirus vectors expressing p53 in E1-transformed cells. 911 cells were infected with adenovirus vectors as ... König C., Roth J., Dobbelstein M. Adenovirus type 5 E4orf3 protein relieves p53 inhibition by E1B-55- kilodalton protein. J. ... The large E1B protein together with the E4orf6 protein target p53 for active degradation in adenovirus infected cells. Oncogene ...
Adenovirus serotype 5 (Ad5) has been widely used in clinical trials because it expresses inserted transgenes robustly and ... adenovirus vector persists despite deletion of the preterminal protein. J Gene Med 2:250-259CrossRefPubMedGoogle Scholar ... Anti-tumor immunotherapy despite immunity to adenovirus using a novel adenoviral vector Ad5 [E1-, E2b-]-CEA. ... Production and characterization of improved adenovirus vectors with the E1, E2b, and E3 genes deleted. J Virol 72:926-933PubMed ...
a. The LTR and E1 protein. b. The ITR and packing signal. c. At least one LTR and an ITR. d. E1, E3 and E4 ... a. Adenovirus vectors have a lower titre than Adeno-associated vectors. b. Adeno-associated vectors can only carry a small ... d. Structural proteins can only be expressed from the same plasmid and this is aided through the use of packing cells with ... c. It is based on the rec L1 capsid protein which can be expressed in yeast. d. It can aid in wart regression and protection ...
E1, E2, E4, and E5; and a late region with two genes, L1 (major capsid protein) and L2 (minor capsid protein) [17]. These ... HPV is a double-stranded nonenveloped DNA adenovirus, which belongs to a large family of more than 130 genotypes [19, 20]. The ... A large body of evidence suggests that oncogenic properties of high-risk HPV are related to the E6 and E7 proteins and their ... along with abundant expression of the E6 and E7 proteins [17]. In the superficial layers of the epithelium, the L1 and L2 ...
Protein concentrations were determined using the Bradford reagent (Bio-Rad). Samples (30 μg of protein per lane) were loaded on ... 3. The method of claim 1, wherein said vector is an adenovirus. 4. The method of claim 1, wherein said condition is asthma. 5. ... The E1/E3-deleted replication-deficient recombinant Ad was made using the AdEasy system (He 1998) (Quantum Biotechnologies, ... 8: Effect of WT-AdIGFBP-3 and m-AdIGFBP-3 administration on VCAM-1 and ICAM-1 protein expression. A. VCAM-1 and ICAM-1 protein ...
Helper-dependent adenovirus vectors (HDVs) are safe and efficient tools for gene transfer with high cloning capacity. However, ... The higher viral protein content in HDV-producing cells was also consistent with an increased activation of autophagy and cell ... When compared with E1-deleted (ΔE1) vectors, we found a faster helper genome replication during HDV production. This was ... Helper-dependent adenovirus vectors (HDVs) are safe and efficient tools for gene transfer with high cloning capacity. However, ...
The 293 and 911 cells constitutively express the E1 gene products required for propagation of all recombinant adenoviruses, ... Fifth, some of the new vectors contain a green fluorescent protein (GFP) gene incorporated into the adenoviral backbone, ... In most recombinant vectors, transgenes are introduced in place of E1 or E3, the former supplied exogenously. The E1 deletion ... A simplified system for generating recombinant adenoviruses. Tong-Chuan He, Shibin Zhou, Luis T. da Costa, Jian Yu, Kenneth W. ...
Timely Synthesis of the Adenovirus Type 5 E1B 55-Kilodalton Protein Is Required for Efficient Genome Replication in Normal ... The 293 and 911 cells constitutively express the E1 gene products required for propagation of all recombinant adenoviruses, ... The Repression Domain of the E1B 55-Kilodalton Protein Participates in Countering Interferon-Induced Inhibition of Adenovirus ... In most recombinant vectors, transgenes are introduced in place of E1 or E3, the former supplied exogenously. The E1 deletion ...
The present invention provides a hybrid vector construct which comprises a portion of an adenovirus, 5 and 3 ITR sequences ... The relevance of including an E1 deleted adenovirus here is to document that the level of adenovirus E1 proteins expressed in ... the adenovirus sequence may contain deletions of the E1 genes and the E3 gene, or of the E1, E2a and E3 genes, or of the E1 and ... Alternatively, the low levels of rep expression from P5 that occur in the absence of adenovirus E1 proteins may be sufficient ...
E1-, E2a-, and E3-represent the deletions of these genes from the adenovirus genome. The insertion of the human GKRP expression ... The resulting proteins, and/or the viral capsid proteins necessary for transduction, may directly affect host cell functions or ... 29.1 ± 0.3; P , 0.05). Therefore, it seems that increasing GKRP protein levels allows increased GK protein and activity levels ... To examine the effect of GKRP overexpression on GK protein levels, we performed Western blot analysis on protein lysates ...
Human adenovirus type 5 vectors deleted of early region 1 (E1) undergo limited expression of early replicative E2 proteins and ... Adenovirus-Mediated Expression of the p14 Fusion-Associated Small Transmembrane Protein Promotes Cancer Cell Fusion and ... A reduction in the human adenovirus virion size through use of a shortened fibre protein does not enhance muscle transduction ... Expression of the fusogenic p14 FAST protein from a replication-defective adenovirus vector does not provide a therapeutic ...
Results: The results indicated that METH increased the protein levels of LC3B and Beclin-1, and these effects were ... The results indicated that METH increased the protein levels of LC3B and Beclin-1, and these effects were significantly ... We evaluated the effects of the single or combined treatment of METH and HIV-Tat on the protein expressions of the autophagy- ... We evaluated the effects of the single or combined treatment of METH and HIV-Tat on the protein expressions of the autophagy- ...
... the Merck rAD5 vector is missing only one adenovirus protein (E1) and thus expresses more viral genes than other adenovirus ... Neutralizing antibodies to adenovirus serotype 5 vaccine vectors are directed primarily against the adenovirus hexon protein. J ... The vector used by National Institutes of Healths Vaccine Research Center, for example, lacks both the E1 and E3 protein. ... Adenovirus types 5 and 35 seroprevalence in AIDS risk groups supports type 35 as a vaccine vector. AIDS. 18:1213-1216. ...
For comparative purposes an adenovirus in which the E1 region is regulated by a constitutively active CMV promoter (Ad-CMV-E1) ... 9. The recombinant virus of claim 6 wherein the peptide is adenovirus death protein (ADP). 10. The recombinant virus of claim 7 ... dl309 is the cell line infected with adenovirus which is wild-type in the E1 region and has mutations in the E3 region, CMV-E1 ... The genomic E1 region from nucleotides 501 to 4105 of adenovirus type 5 (encompassing the E1A gene region and the E1B and IX ...
Additionally, E1/E3-deleted adenoviruses do express adenoviral proteins encoded within their genomes (Yang et al., 1994a,b). ... Tumor-specific, replication-competent adenovirus vectors overexpressing the adenovirus death protein. J. Virol. 2000;74(13): ... 3) Following peripheral immunization against adenovirus type 5, transgene expression from an E1/E3-deleted adenovirus vector is ... Expression from an E1/E3-deleted adenovirus in brains of animals previously immunized against adenovirus type 5 is almost ...
E1) proteins and isolation of E1 expressing cell lines. Virology 295:108-118.. ... Bovine adenovirus type 3 internalization is independent of primary receptors of human adenovirus type 5 and porcine adenovirus ... Aggarwal, N. and Mittal, S. K. (2000). Sequence analyses of porcine adenovirus type 3 E1 region, pIX, and pIVa2 genes. ... Breker-Klassen, M. M., Yoo, D., Mittal, S. K., Sorden, S. D., Haines, D. M. and Babiuk, L. A. (1995). Recombinant adenoviruses ...
Induction of CD8+ T cells to an HIV-1 antigen through a prime boost regimen with heterologous E1-deleted adenoviral vaccine ... Neutralizing Antibodies to Adenovirus Serotype 5 Vaccine Vectors Are Directed Primarily against the Adenovirus Hexon Protein. ... Neutralizing Antibodies to Adenovirus Serotype 5 Vaccine Vectors Are Directed Primarily against the Adenovirus Hexon Protein ... Neutralizing Antibodies to Adenovirus Serotype 5 Vaccine Vectors Are Directed Primarily against the Adenovirus Hexon Protein ...
... adenovirus-mediated transfer of human acid-alpha-glucosidase gene into glycogen storage disease type II knockout mice were ... The persistence of the protein also correlated with long-term correction of pathologic intramuscular glycogen accumulations in ... TY - JOUR T1 - Long-term efficacy after [E1-, polymerase-] adenovirus-mediated transfer of human acid-alpha-glucosidase gene ... Long-term efficacy after [E1-, polymerase-] adenovirus-mediated transfer of human acid-alpha-glucosidase gene into glycogen ...
HEK293 cells constitutively produce the Ad5 E1 protein (41), allowing the Ad5[E1−] vaccine platform but not the Ad5[E1−,E2b−] ... Multiply deleted [E1, polymerase-, and pTP-] adenovirus vector persists despite deletion of the preterminal protein. J Gene Med ... Recombinant adenovirus vaccine vector construction.Transgene-lacking recombinant Ad5 vector platforms Ad5[E1−] (64) and Ad5[E1 ... Neutralizing antibodies to adenovirus serotype 5 vaccine vectors are directed primarily against the adenovirus hexon protein. J ...
Differential transformation of primary human embryo retinal cells by adenovirus E1 regions and combinations of E1A and ras. ... Molecular cloning and functional analysis of the adenovirus E1A-associated 300-kD protein (p300) reveals a protein with ... that give rise to protein products of 243 and 289 amino acids, respectively (in adenovirus types 2 and 5 [Ad2/5]). The protein ... Mapping of cellular protein binding sites on the products of early region 1A of human adenovirus type 5. Mol. Cell. Biol. 8: ...
An oncolytic adenovirus 11p vector expressing adenovirus death protein in the E1 region showed significant apoptosis and tumour ... Replication-competent adenovirus 11p vector armed with ADP gene at E1 region significantly improved tumour-killing effect on ... Completely replication-competent adenovirus 11p GFP (RCAd11pGFP) vector with an insertion upstream of the full-length E1 region ... Complete replication-competent adenovirus 11p vectors with E1 or E3 insertions show improved heat stability ...
CAR 3 is a 46-kDa transmembrane protein that enables virus attachment via interaction with the adenovirus fiber-knob protein. ... HEK293 cells (human embryonic kidney cells expressing the E1 region of adenovirus type 2) were incubated with serial dilutions ... Infectivity with adenovirus was abolished in cells that had been preincubated with a recombinant adenovirus fiber protein (a ... we found increased expression of the early adenovirus protein E1A in cells infected with wild-type adenovirus after treatment ...
  • Adenovirus serotype 5 (Ad5) has been widely used in clinical trials because it expresses inserted transgenes robustly and augments the innate immune response. (springer.com)
  • Porcine adenovirus serotype 3 internalization is independent of CAR and avb3 or avb5 integrin.Virology 332:157-166. (purdue.edu)
  • The study and characterization of recombinant adenovirus (Ad) vaccine platforms, particularly Ad serotype 5 (Ad5) vaccine platforms, are of great interest for their development and clinical application for human vaccination. (asm.org)
  • There have been several concerns about the use of recombinant adenovirus serotype 5 (rAD5) as a vaccine vector, including preexisting vector-specific immunity and uncertainty about whether these vectors can induce long-lasting, broad, and protective immune responses ( 20 - 22 ). (rupress.org)
  • The genome of the most commonly used human adenovirus (serotype 5) consists of a linear, 36-kb, double-stranded DNA molecule. (pnas.org)
  • The hAd5-S-Fusion+N-ETSD Vaccine is a human adenovirus serotype 5 (hAd5) vector with E1/E2b/E3 deletions expressing SARS-CoV-2 viral antigen spike fusion protein and nucleocapsid with an enhanced T-cell stimulation domain. (clinicaltrials.gov)
  • Generx is biologically engineered using an E1-region deleted, replication-deficient adenovirus serotype 5 vector to deliver the 621 base pair gene encoding human fibroblast growth factor-4 (FGF-4) under the control of a modified cytomegalovirus (CMV) promoter. (medindia.net)
  • Our previously constructed recombinant adenovirus expressing E3-E2-6K genes from VEEV serotype IA/B (RAd/VEEV#3) was able to confer 90-100% protection against 100LD 50 of strains IA/B, ID and IE of VEEV. (biomedcentral.com)
  • Ls are zinc proteases which cleave plasma membrane or vesicle associated Soluble N -ethylmaleimide-sensitive factor activating protein receptor (SNARE) proteins, based upon the serotype [ 4 ]. (mdpi.com)
  • E1-deletion-containing replication-defective adenoviral recombinants based on human serotype 5 (Adhu5) have been tested widely as carriers for gene therapy (2, 21). (aksumuniversity.org)
  • We demonstrated previously how the effectiveness of Adhu5 recombinant vaccines was impaired in mice which got had prior contact with the same serotype of adenovirus. (aksumuniversity.org)
  • This serotype of adenovirus will not circulate in human beings and does not have neutralizing B-cell epitopes cross-reacting with those of common Sotrastaurin human being serotypes (7). (aksumuniversity.org)
  • Replication-defective adenovirus serotype 5 vectors elicit durable cellular and humoral immune responses in nonhuman primates. (harvard.edu)
  • In human AdV serotype 5 (HAdV5), the IVa2 protein initiates specific recognition of viral genome by associating with the viral packaging domain located between nucleotides 220 and 400 of the genome. (microbiologyresearch.org)
  • We report here a novel vector (Av4 orf 3nBg) lacking E1, E2a, and all of E4 except open reading frame 3 (ORF3) and expressing a β-galactosidase reporter gene. (asm.org)
  • This vector was generated by transfection of a plasmid carrying the full-length vector sequence into A30.S8 cells that express E1 and E2a but not E4. (asm.org)
  • One is based on the concept that removal of all coding sequences for viral proteins from the vector backbone renders the vector less immunogenic and restricts the immune response to the injected viral capsid proteins. (asm.org)
  • A second alternative in decreasing adenovirus vector immunogenicity and toxicity is based on a gene attenuation strategy wherein the sequential removal of key early genes is anticipated to reduce expression from other essential genes. (asm.org)
  • Recently, it has been demonstrated that additional deletion of the E4 region in an E1 deletion background has a major influence on the stability of the adenovirus vector genome in addition to prolonging transgene expression ( 2 , 10 , 16 , 40 ). (asm.org)
  • The vector used for this trial was based on human adenovirus type 5, deleted in E1 and E4, and contained human OTC cDNA. (nih.gov)
  • Expression of the late viral gene product, hexon protein, was observed in C3H and C57BL/6 mice injected with E4 wild-type adenovirus constructs Adv-cmv-beta-Gal (BG), Adv-cmv-hp53 (WT), and empty E1- vector Adv-E4 (EW) 3 to 28 days after injection, but was undetectable in mice treated with E4 modified empty E1- vector Adv-GAL4 (EG) or Adv-cmv-hp53-GAL4 (G4). (nih.gov)
  • Multiple re-immunizations using the same vector platform are now possible with the novel Ad5 [E1-, E2b-] platform. (springer.com)
  • 3. The method of claim 1, wherein said vector is an adenovirus. (freepatentsonline.com)
  • In a mouse model of GSD-II, we demonstrate that infection of the murine liver with a modified adenovirus (Ad) vector encoding human GAA (hGAA) resulted in long-term persistence of the vector in liver tissues for at least 6 months. (unboundmedicine.com)
  • The persistence of the protein also correlated with long-term correction of pathologic intramuscular glycogen accumulations in all muscle groups tested, but most notably the cardiac tissues, which demonstrated a significantly decreased glycogen content for at least 190 days after a single vector injection. (unboundmedicine.com)
  • The present invention provides a hybrid vector construct which comprises a portion of an adenovirus, 5' and 3' ITR sequences from an AAV, and a selected transgene. (google.com)
  • 3. The vector according to claim 2 wherein said adenovirus sequences further comprise a functional deletion in the E1 gene. (google.com)
  • 4. The vector according to claim 2 wherein said adenovirus sequences further comprise functional deletions in one or more adenovirus genes selected from the group consisting of: the E2a gene, the E4 gene, the late genes L1 through L5, and the intermediate genes IX and IV a . (google.com)
  • 8. The vector according to claim 5 wherein said reporter gene is selected from the group consisting of the genes encoding β-galactosidase, alkaline phosphatase and green fluorescent protein. (google.com)
  • To further explore the physiological role of GKRP in vivo, we used an E1/E2a/E3-deficient adenoviral vector containing the cDNA encoding human GKRP (Av3hGKRP). (diabetesjournals.org)
  • Registered on 11 August 2020 by the Russian Ministry of Health as Gam-COVID-Vac (Russian: Гам-КОВИД-Вак, romanized: Gam-KOVID-Vak), Sputnik V is an adenovirus viral vector vaccine. (wikipedia.org)
  • Gam-COVID-Vac is a viral two-vector vaccine based on two human adenoviruses - a common cold virus - containing the gene that encodes the full-length spike protein (S) of SARS-CoV-2 to stimulate an immune response. (wikipedia.org)
  • A) Schematic overview of ANCHOR3 encoding AdV vector with E1/E3 deleted. (asm.org)
  • Bovine adenoviral vector-based H5N1 influenza vaccine overcomes exceptionally high levels of pre-existing immunity against human adenovirus. (purdue.edu)
  • What is a feature of the gutless adenovirus expression vector? (brainscape.com)
  • More alarmingly, the vaccine appeared to increase the rate of HIV infection in individuals with prior immunity against the adenovirus vector used in the vaccine. (rupress.org)
  • A nonreplicating adenovirus vector encoding β-galactosidase (rAd-βgal) was administered intravenously to determine the relationship between circulating AdNAb titer and vector gene expression in the liver. (aacrjournals.org)
  • The death in the USA of an 18 year old with ornithine transcarbamylase (OTC) deficiency after intrahepatic arterial injection of an adenovirus vector carrying a wild-type version of the defective enzyme has precipitated a flurry of reports and congressional hearings focusing on the ethics of such trials and on the very nature of clinical research itself. (bmj.com)
  • The two dual AAV vector systems evaluated for the delivery of CEP290 were unable to mediate full-length transcript and protein expression in vitro. (ufl.edu)
  • In our system, transcriptional targeting of solitary viral E1A resulted in 50% enhanced restricted vector replication when compared with an unrestricted replication-competent adenovirus. (aacrjournals.org)
  • Leveraging the knowledge that E1−E3− adenovirus (Ad) gene transfer vectors are potent immunogens, we have developed a novel vaccine platform for addictive drugs by covalently linking a cocaine analog to the capsid proteins of noninfectious, disrupted Ad vector. (drugs-forum.com)
  • Below is a selection of publications that are associated with Etubics next generation adenovirus vector. (etubics.com)
  • Control of SIV Infection and subsequent induction of pandemic H1N1 immunity in rhesus macaques using an Ad5[E1,E2b-] vector platform. (etubics.com)
  • Optimization of vaccine responses with an E1, E2b and E3- deleted Ad5 vector circumvents pre-existing anti-vector immunity. (etubics.com)
  • Customer provide adenovirus vector or select our pre-packaged adenovirus vectors. (signagen.com)
  • These vaccines include DNA-based, viral vector-based, and recombinant protein-based vaccines. (mdpi.com)
  • We have now used these packaging cells to construct a new adenoviral vector (Ad5.betagal.DeltaF) with E1, E3, and L5 (fiber) deleted and analyzed the fiber null phenotype. (scripps.edu)
  • Here, we used an adenoviral (Ad) vector encoding the Ca 2+ chelator protein parvalbumin (PV) fused to a nuclear exclusion signal (NES) and the Discosoma red fluorescent protein (DsRed) to investigate the function of cytoplasmic Ca 2+ signals on MSC proliferation. (biomedcentral.com)
  • The adenovirus vector is able to deliver genes with 100% efficiency to a wide selection of cell types including dividing and non-dividing cells, primary cells, or cell lines. (vectorbiolabs.com)
  • All these have made recombinant adenoviruses the vector of choice for functional genomics research. (vectorbiolabs.com)
  • We now offer adenovirus vector design, custom cloning and production services. (oxfordgenetics.com)
  • So whether you need to create an adenovirus vector from scratch or you wish to optimise an existing system then contact the experts and let us help you. (oxfordgenetics.com)
  • So, instead of just telling you about the vaccine, I want to tell you some of the adenovirus vector background (hope you'll stick around! (thebumblingbiochemist.com)
  • Adenovirus vector-based purging of multiple myeloma cells. (harvard.edu)
  • Adenovirus vectors are currently used for gene transfer in basic research and gene therapy protocols due to their high titers, ability to target a wide range of both dividing and nondividing cells, and mediation of high-level foreign protein expression without replication or integration of the viral genome ( 4 , 18 , 36 , 37 ). (asm.org)
  • 14 Shuttle plasmids were cotransfected into CRE8 cells (expressing Cre recombinase) with E1- and E3-deleted adenovirus-5 genome DNA (Ψ5). (ahajournals.org)
  • The methods range from how to grow and titer adenoviruses and how to construct specific alterations in the adenovirus genome, to how to measure apoptosis induced by cells of the immune system, cytokines, and intrinsic apoptosis effectors. (springer.com)
  • When compared with E1-deleted (ΔE1) vectors, we found a faster helper genome replication during HDV production. (biomedsearch.com)
  • The uncoupling between high-number genome copies of adenoviruses detected by real-time PCR and the absence of infectivity detected by tissue culture suggests that genome-based detection methods are inadequate for direct assessment of human health risk. (asm.org)
  • One embodiment of the invention is directed towards a method of inactivating a viral genome in a cell that includes administering to the cell comprising an engineered zinc finger protein and a DNA-cleavage domain, wherein the engineered zincfinger proteins (ZFPs) comprise a plurality of zinc fingers. (patentgenius.com)
  • The main difference is that lentivirus integrates into the host genome and adenovirus does not. (thermofisher.com)
  • The E1 region of the genome has been divided into two major transcriptional units, E1A and E1B, each expressing proteins of the same name (ADENOVIRUS E1A PROTEINS and ADENOVIRUS E1B PROTEINS). (bvsalud.org)
  • The entire adenovirus genome is a linear, double-stranded DNA of approximately 36Kb. (vectorbiolabs.com)
  • Adenovirus vectors can be generated by insertion or deletion of DNA in three regions, namely the E1 gene, E3 gene and a short segment between the E4 gene and the end of the viral genome. (oxfordgenetics.com)
  • The adenovirus genome comprises of multiple genes that are transcribed at different time points in the viral replication cycle. (oxfordgenetics.com)
  • Genome structure of incomplete particles of adenovirus. (microbiologyresearch.org)
  • Recent efforts to improve adenovirus vectors for in vivo use have focused on the sequential deletion of essential early genes. (asm.org)
  • Abstract- As distal targets and mediators of signal transduction pathways, activator protein-1 (AP-1), c-Jun, and c-Fos are among the primary regulators of genes involved in cell function, proliferation, and differentiation. (ahajournals.org)
  • The adenovirus E1A-associated 130-kD protein is encoded by a member of the retinoblastoma gene family and physically interacts with cyclins A and E". Genes Dev. (wikipedia.org)
  • We evaluated the effects of the single or combined treatment of METH and HIV-Tat on the protein expressions of the autophagy-related genes, including Beclin-1 and LC3B, ATG5, and ATG7 in METH and HIV-Tat-induced autophagy. (frontiersin.org)
  • In Ad5-gfa2(B)3-E1, the E3 region was deleted to create space for future insertion of heterologous therapeutic genes. (eur.nl)
  • Strategies to further improve the efficacy of Ad5-gfa2(B)3-E1 for the treatment of malignant gliomas include the insertion of therapeutic genes in E3 or retargeting to receptors that are more abundantly expressed on primary glioma cells than CAR. (eur.nl)
  • Berkner, "Development of Adenovirus Vectors for the Expression of Heterologous Genes," BioTechniques 6(7): 616-629, 1988. (patentgenius.com)
  • Heterologous genes are cloned in place of E1, E3 and E4 c. (brainscape.com)
  • LoxP sites are recognised by cre-recombinase to excise adenovirus genes c. (brainscape.com)
  • The insert sizes are smaller than normal adenovirus vectors due to the removal of immunogenic genes d. (brainscape.com)
  • The invention also includes constructs useful for screening of agents which interact with proteins or genes in the hypoxia-inducible pathway or are jointly translated under hypoxia and animal models useful for monitoring a variety of hypoxic conditions in a non-invasive manner. (freepatentsonline.com)
  • For example, adenoviruses transfer genes to a broad spectrum of cell types, and gene transfer is not dependent on active cell division. (pnas.org)
  • Of these, the ability of adenoviruses to efficiently infect and deliver genes to a range of cells and to be generated to high titres has led to their widespread application. (bmj.com)
  • 4 They have been modified by deletion of the E1 region, which encodes proteins that regulate expression of the other early genes (as well as the late virus structural proteins), thereby rendering virus replication defective. (bmj.com)
  • Bone morphogenetic proteins (BMPs) are dimeric proteins that bind to type I and type II BMP receptors, transducing signals through small mothers against decapentaplegic (Smad)-dependent and -independent pathways to regulate the transcription of BMP target genes ( 8 ). (spandidos-publications.com)
  • In addition to Smad3, accumulating data suggest that protein kinase C (PKC) pathways might converge to modulate TGFβ target genes. (ahajournals.org)
  • No amino acid residues were released from the E1 protein by digestion with a mixture of carboxypeptidases A and B. These results confirm that the structural proteins of RV are translated from three genes corresponding to C, E2 and E1. (microbiologyresearch.org)
  • Optimisation of genes has been shown to be beneficial for expression of proteins in a range of applications. (biomedcentral.com)
  • Optimisation has increased protein expression levels through improved codon usage of the genes and an increase in levels of messenger RNA. (biomedcentral.com)
  • This study, in which the utility of optimising genes encoding the structural proteins of VEEV is demonstrated for the first time, informs us that including optimised genes in gene-based vaccines for VEEV is essential to obtain maximum immunogenicity and protective efficacy. (biomedcentral.com)
  • For example, cancer cells in which the tumor suppressor proteins p53 or pRb are inactivated were reported to be permissive for efficient replication of adenoviruses that are mutated in the E1B55K or E1A genes that encode p53- or pRb-inactivating proteins, respectively ( 7 -9 ). (aacrjournals.org)
  • The E2 proteins provide the machinery for viral DNA replication and transcription of late genes. (vectorbiolabs.com)
  • To accommodate larger recombinant genes (up to 8 Kb), 1st generation adenoviruses are both E1 and E3 deleted (E1/E3), since the E3 region is not essential for in vitro viral growth. (vectorbiolabs.com)
  • Second and third generation adenovirus vectors were developed by deleting E1, E2 and E4 genes. (oxfordgenetics.com)
  • Genes transcribed early in the viral replication cycle are the E1, E2, E3 and E4 genes. (oxfordgenetics.com)
  • If this using-virus-to-deliver-genetic-info thing could be done, it would offer up a huge range of possibilities, because stretches of that genetic info called genes are like recipes for making proteins. (thebumblingbiochemist.com)
  • So you could get cells to make proteins that they have typo-ed recipes for (i.e. mutated genes), treating (or even curing depending on the circumstances) genetic diseases - or you could get cells to make viral proteins they could learn to recognize as foreign and produce antibodies against. (thebumblingbiochemist.com)
  • Type II CRAds are adenoviruses where the expression of genes essential for adenoviral replication are under the control of a tumor-specific promoter. (barnardhealth.us)
  • The proteins are involved in both positive and negative regulation of expression in viral and cellular genes, and also affect the stability of viral mRNA. (curehunter.com)
  • Surprisingly, even strong overexpression of p53 or p53mt24-28 allowed the virus to replicate as efficiently as in the absence of p53 proteins, both in tumor cells and in primary endothelial cells. (aacrjournals.org)
  • However, their efficacy depends on the ability of tumor cells to bind and internalize adenovirus. (aacrjournals.org)
  • Oncolytic adenoviruses act by replicating within and lysing tumor cells. (aacrjournals.org)
  • Many levels in adenovirus replication may be regulated to generate conditionally replication-competent adenoviruses targeting tumor cells. (aacrjournals.org)
  • Evidence suggests that interfering with either the expression of the livin gene or the function of the livin protein could provide a potential therapeutic avenue to induce apoptosis in tumor cells and to significantly improve antitumor responses ( 13 - 15 ). (spandidos-publications.com)
  • Adenovirus vectors have been constructed with the E1 gene deleted and with this deletion in combination with an E2a, E2b, or E4 deletion. (asm.org)
  • This study suggests that the additional deletion of E4 in an E1 and E2a deletion background may be beneficial in decreasing immunogenicity and improving safety and toxicity profiles, as well as increasing transgene capacity and expression for liver-directed gene therapy. (asm.org)
  • Recently, we extended those studies by semiquantifying the levels of early and late transcripts in an adenovector with a double deletion of E1 and E2a in vitro and in vivo. (asm.org)
  • Furthermore, it is controversial whether adenoviruses with a deletion in the E1B-55 kDa-coding region might selectively replicate in cells with a mutation or deletion of the p53 gene and, therefore, represent a tool in cancer therapy. (aacrjournals.org)
  • Experiments with d17001, an E3-deleted variant of wtAd5, confirmed that the specificity of Ad5-gfa2(B)3-E1 replication was based on the promoter driving E1 and not on the E3 deletion. (eur.nl)
  • A related tumor-targeting strategy based on the complementation of viral E1A conserved region 2 deletion (E1Δ24), which is necessary for retinoblastoma protein binding, has recently been described ( 7 ). (aacrjournals.org)
  • It is now well established that loss of p53 function by gene mutation, deletion, protein degradation, or viral oncogene binding plays a central role in cancer development. (biologists.org)
  • Deletion of the E1 gene inhibits viral replication inside host cells. (oxfordgenetics.com)
  • Recombinant adenoviruses are presently being tested clinically as a new strategy for the treatment of cancer. (aacrjournals.org)
  • Recombinant adenoviruses provide a versatile system for gene expression studies and therapeutic applications. (pnas.org)
  • This system should expedite the process of generating and testing recombinant adenoviruses for a variety of purposes. (pnas.org)
  • Recombinant adenoviruses currently are used for a variety of purposes, including gene transfer in vitro , vaccination in vivo , and gene therapy ( 1 - 4 ). (pnas.org)
  • Two approaches traditionally have been used to generate recombinant adenoviruses. (pnas.org)
  • It was envisaged that the inability of these recombinant adenoviruses to replicate efficiently would prevent the production of unwanted viral proteins by infected cells, thus limiting both direct adenovirus toxicity and possible harmful consequences of antiadenovirus immune responses. (bmj.com)
  • A2AR, a G protein-coupled receptor, is highly expressed on the cell surfaces of T-cells and, upon activation by adenosine, inhibits their proliferation and activation. (cancer.gov)
  • Upon subcutaneous administration, abaloparatide acts similar to PTHrP and targets, binds to and activates parathyroid hormone 1 (PTH1) receptor (PTH1R), a G protein-coupled receptor (GPCR) expressed in osteoblasts and bone stromal cells. (cancer.gov)
  • An important determining factor for the successful entry of such adenoviruses into target cells is expression of the coxsackievirus and adenovirus receptor (CAR) at the cell surface. (aacrjournals.org)
  • Blunted responsiveness of the β-adrenergic receptor system, with downregulation of β-adrenergic receptors, increased activity of G protein-coupled receptor kinase 1, decreased stimulability of adenylyl cyclase, 13 and various alterations of calcium cycling are key features of autonomic nervous system dysregulation. (ahajournals.org)
  • The Smads are a series of proteins downstream from the TGFβ receptor that transduces its signal to the nucleus. (ahajournals.org)
  • Adenovirus requires that host cells have the CAR receptor for efficient transduction, whereas due to the VSVG membrane coat on lentivirus particles, these viruses have broad tropism for a variety of mammalian cell types. (thermofisher.com)
  • Cotten M, Wagner E, Zatloukal K, Phillips S, Curiel DT, Bimstiel ML, (1992) High-efficiency receptor-mediated delivery of small and large (48kb) gene constructs using the endosome disruption activity of defective or chemically inactivated adenovirus particles. (springer.com)
  • Cotten M, Wagner E, Zatloukal K, Bimstiel ML (1993b) Chicken adenovirus (CELO virus) particles augment receptor-mediated DNA delivery to mammalian cells and yield exceptional levels of stable transformants. (springer.com)
  • The prime receptor for human adenoviruses is the Coxsackie/Adenovirus Receptor (CAR). (vectorbiolabs.com)
  • In addition, adenoviruses that enter cells primarily, although not exclusively, through interaction with the coxsackie-adenovirus receptor (CAR) (3) efficiently transduce dendritic cells (27), which are the main cell population able to present antigen to a na?ve immune system. (aksumuniversity.org)
  • Fusion proteins including an IL-17 receptor with a multimerization domain, or an IL-23 receptor and a multimerization domain, and recombinant viral vectors encoding such fusions, are described. (patentsencyclopedia.com)
  • 43. The fusion protein of claim 40, wherein the interleukin receptor is a soluble interleukin receptor. (patentsencyclopedia.com)
  • The first lentiviral vectors were derived from human immunodeficiency virus-1 (HIV-1) but were pseudotyped by using the envelope glycoproteins from other viruses such as the vesicular stomatitis virus G protein (VSV-G), a fusion protein used to improve infection efficiency. (pubmedcentralcanada.ca)
  • In vitro hPBMC infection with the Ad5[E1−,E2b−] vaccine also provoked greater Th1-dominant gene responses yet smaller amounts of Ad-derived gene expression than Ad5[E1−] vaccines. (asm.org)
  • This raises the possibility that those tumors representing the greatest therapeutic challenge might be the least susceptible to infection with therapeutic adenoviruses. (aacrjournals.org)
  • Infection of a GFAP-positive cell line with Ad5-gfa2(B)3-E1 resulted in E1A and E1B expression at 75% and 30% of the levels obtained after wtAd5 infection. (eur.nl)
  • The scientific community has recently learned that the STEP HIV vaccine trial, which used an rAd5-based vaccine developed by Merck, failed to protect Ad5-seronegative individuals against infection and may even have enhanced infection in vaccinees with prior immunity to adenoviruses ( 23 - 26 ). (rupress.org)
  • However, when expression of p53 was increased following infection with mutant adenoviruses, which do not express the larger E1B proteins, there was an appreciable increase in p53 transcriptional activity and in the levels of all of the MDM2 components. (nih.gov)
  • Fifth, some of the new vectors contain a green fluorescent protein (GFP) gene incorporated into the adenoviral backbone, allowing direct observation of the efficiency of transfection and infection, processes that have been difficult to follow with adenoviruses in the past. (pnas.org)
  • Antibodies to adenoviruses are common in the human population due to natural infection. (aacrjournals.org)
  • The commonly used first generation recombinant adenovirus vectors are based on adenovirus type 5, which causes mild respiratory infection in humans. (bmj.com)
  • In practice this has not always been the case and studies in rodents, primates, and humans have provided variable results, highlighting the need for a more detailed understanding of the natural history of adenovirus infection in humans and questioning the value of animal models in determining the safety of virus vectors. (bmj.com)
  • Using a recombinant adenovirus to deliver Ag, we demonstrated that core(+) mice failed to clear adenovirus-LacZ (Ad-LacZ) infection in the liver. (jimmunol.org)
  • 2011). These studies have also determined that the adenovirus E1B55K-binding protein, E1B-AP5 (also known as hnRNPUL1) is an integral component of ATR signalling pathways activated during infection (Blackford et al . (birmingham.ac.uk)
  • In order to determine to what extent adenovirus targets the ubiquitin-proteasome pathway during infection we also employ proteomic techniques in order to identify those proteins whose expression is affected significantly by adenovirus infection or during the process of adenovirus-mediated cellular transformation. (birmingham.ac.uk)
  • Furthermore, replication-competent adenoviral vectors promise to be more efficient gene delivery vehicles than their replication-deficient counterparts ( 4 ), and they are also associated with a higher risk for adverse effects, especially due to the lack of an established effective therapy for serious, disseminated adenovirus infection ( 5 ). (aacrjournals.org)
  • Following adenovirus infection, E1A is the first viral gene product to be expressed, and is required for the efficient transcription of all other viral early gene regions ( 12 ). (aacrjournals.org)
  • Like the E1 gene products, the adenoviral E4 gene region is expressed early after infection and is an essential component of the viral life cycle. (aacrjournals.org)
  • Several years ago it was reported that the entry of adenovirus particles could augment the uptake of polylysine-condensed DNA molecules ( Curiel et al. (springer.com)
  • Second, high titers of adenovirus particles are easy to produce and are stable. (aacrjournals.org)
  • This was consistent with an upregulation of the Ad polymerase and pre-terminal protein and led to higher and earlier expression of structural proteins. (biomedsearch.com)
  • Conversely, multiply deleted recombinant Ads have further removal of early gene regions, such as E2b (Ad5[E1−,E2b−]), a gene that typically encodes the DNA polymerase and preterminal region of Ad5 ( 1 , 27 ). (asm.org)
  • By using adenovirus-mediated gene transfer, we show that overexpression of AP-1 proteins directly causes coinduction of gene expression of an adhesion molecule, intercellular adhesion molecule-1 (ICAM-1), and a chemokine, monocyte chemoattractant protein-1 (MCP-1), in human vascular endothelial cells (ECs). (ahajournals.org)
  • In an in vivo ectopic bone formation model, the adenovirus‑mediated overexpression of BMP7 enhanced bone formation from CD105+ hDDFCs. (spandidos-publications.com)
  • In summary, local overexpression of uptake-1 in the myocardium results in marked structural and functional improvement of heart failure, thus underlining the importance of uptake-1 as a key protein in heart failure. (ahajournals.org)
  • Inhibition of PKCδ activity by rottlerin or dominant-negative adenovirus (AdPKCδ DN) blocked TGFβ1's induction of FN, whereas overexpression of PKCδ enhanced TGFβ's effect. (ahajournals.org)
  • Pathologic findings from the national surgical adjuvant breast and bowel project: prognostic significance of erbB-2 protein overexpression in primary breast cancer. (springer.com)
  • Adenovirus-mediated overexpression of a c-Cbl mutant that ablates the major tyrosine phosphorylation sites attenuated the Akt and the mammalian target of rapamycin activation and decreased the proliferation and migration of smooth muscle cells in response to PDGF-BB or fibroblast growth factor. (ahajournals.org)
  • A recombinant adenovirus with deleted E1 and E3, and E4-inactivated by replacing the E4 promoter with a synthetic promoter composed of a minimal TATA box and five consensus yeast GAL4-binding site elements was developed and used to express the human tumor suppresser gene p53. (nih.gov)
  • 14 A recombinant adenovirus expressing tTA (AdtTA) was constructed with the tTA cDNA and upstream cytomegalovirus promoter from pUHD15-1. (ahajournals.org)
  • In this construct, the E1 region is under control of the tissue-specific GFAP promoter (gfa2) with three additional copies of the glial specific 'B' enhancer. (eur.nl)
  • To determine the mechanism of the HCV core in suppressing Ag-specific CD8 + T cell responses, we generated a transgenic mouse, core(+) mice, where the expression of core protein is directed to the liver using the albumin promoter. (jimmunol.org)
  • The CMV promoter is capable of driving high levels of transgene protein expression in transfected cells for up to approximately four weeks. (medindia.net)
  • In our current study, we developed oncolytic adenoviruses which preferentially lyse pancreatic and colon cancer cells by replacing viral E1 and/or E4 promoter with the tumor/tissue-specific promoters, cyclooxygenase-2 (COX-2), midkine (MK), or the cell cycle-dependent promoter, E2F1. (aacrjournals.org)
  • In a s.c. xenograft tumor model, there was no significant difference in the antineoplastic efficacy of the double heterologous promoter-controlled vectors when compared with our unrestricted replication-competent control adenovirus or vectors with only E1A transcriptionally driven by a heterologous promoter. (aacrjournals.org)
  • In these adenoviruses, the E4 region (AdΔ24TyrE4) or both E1 and E4 regions (Ad2xTyr) were controlled by a synthetic tyrosinase enhancer/promoter (Tyr2E/P) specific for melanocytes. (aacrjournals.org)
  • To explore the functional consequences and stability of these modifications, we employed targeted installment of DNA methylation and H3K9 methylation in the vascular endothelial growth factor A (VEGF-A) promoter using catalytic domains of DNA or H3K9 methyltransferases that are fused to a zinc finger protein which binds a site in the VEGF-A promoter. (biomedcentral.com)
  • Replication-deficient (E1, E3 deleted) adenoviral vectors containing the rat HGF transgene driven by the human muscle creatine kinase (MCK) promoter were made and obtained from the Institute of Human Gene Therapy, University of Pennsylvania (Adeno-HGF). (ahajournals.org)
  • An adenovirus which has the expression of a replication essential gene under control of the telomerase promoter can therefore replicate in a broad range of tumor types. (barnardhealth.us)
  • In the case of an adenovirus where the expression of the replication essential gene E1 was restricted to melanoma cells using the tyrosinase promoter, replication was shown to be specific for melanoma cells at low infectious units. (barnardhealth.us)
  • The adenovirus L4 33-kilodalton protein binds to intragenic sequences of the major late promoter required for late phase-specific stimulation of transcription. (microbiologyresearch.org)
  • Rarely, Ad5 can acquire the E1 gene from the HEK 293 cells, restoring its ability to replicate. (wikipedia.org)
  • Traditional, or conventional, recombinant Ads are engineered to lack the early E1 and E3 gene regions (Ad5[E1−]), and these deletions substantially inhibit but do not completely eliminate the ability of these viral vectors to replicate and translate viral proteins ( 26 ). (asm.org)
  • Homologous recombination results in a defective adenovirus that can replicate in the packaging line (e.g., 293 or 911 cells) supplying the missing gene products (e.g. (pnas.org)
  • Our previous studies demonstrated that survivin-responsive conditionally replicating adenoviruses regulated with multiple factors (Surv.m-CRAs), which selectively replicate in and kill a broad range of cancer-cell types, are promising anticancer agents. (biomedcentral.com)
  • Adenoviruses need a viral protein called E1 to replicate. (thebumblingbiochemist.com)
  • Those cells can thus make the E1 protein for the virus, the virus will replicate and then burst out and then you can collect that virus (which doesn't have the E1 gene and thus can't replicate) and use it. (thebumblingbiochemist.com)
  • Adenoviruses that can only replicate inside malignant cells are termed conditionally replicating adenoviruses (CRAds). (barnardhealth.us)
  • 6] Thus this adenovirus will replicate inside prostate (tumor) tissue while sparing the other tissues. (barnardhealth.us)
  • The CEA immunogenicity and in vivo anti-tumor effects of repeated immunizations with Ad5 [E1-, E2b-]-CEA compared with those observed with current generation Ad5 [E1-]-CEA were tested in Ad5 pre-immunized mice. (springer.com)
  • In studies comparing these two types of Ads, Ad5[E1−,E2b−] vaccines have been found to promote the decreased expression of viral proteins, persist longer in vivo , and induce less virus-associated toxicity following in vivo administration ( 16 , 28 , 29 ). (asm.org)
  • Adenovirus-Mediated Transfer of a Rcombinant .alpha.1-Antitrypsin Gene to the Lung Epithelium in Vivo," Science 252: 431-434, 1991. (patentgenius.com)
  • While the lack of individual SIBLINGs causes significant mineralization defects in vivo , none of them led to a complete cessation of mineralization suggesting that these proteins have overlapping functions. (pubmedcentralcanada.ca)
  • A xenograft tumor-bearing nude mouse model was developed to assess how a similar in vivo titer would impact the activity of 01/PEME, an oncolytic adenovirus, after intravenous administration. (aacrjournals.org)
  • Conclusions: This report demonstrates for the first time that: (1) a protective epitope of the conserved RSV fusion protein can be mimicked by synthetic peptides: and (2) immunisations with these mimotopes induced specific anti-RSV neutralising antibodies and reduced vital load in vivo. (tudelft.nl)
  • Nevertheless, expression was transient in Bate-Amyloid1-42human vivo due to clearance of adenovirus-infected cells by CD8+ T cells directed against antigens of the adenovirus as well as against the transgene product (4, 26). (aksumuniversity.org)
  • In this study, we demonstrated interactions among IVa2, 33K and DNA-binding protein (DBP) in virus-infected cells by in vivo cross-linking of HAdV5-infected cells followed by Western blot, and co-immunoprecipitation of IVa2, 33K and DBP from nuclear extracts of HAdV5-infected cells. (microbiologyresearch.org)
  • Gaydos CA, Gaydos JC (2004) Adenovirus vaccine. (springer.com)
  • The recombinant adenovirus types 26 and 5 are both used as vectors in the vaccine. (wikipedia.org)
  • HIV DNA-Adenovirus Multiclade Envelope Vaccine Induces gp41 Antibody Immunodominance in Rhesus Macaques. (nih.gov)
  • Development of nonhuman adenoviruses as vaccine vectors. (purdue.edu)
  • These results suggest that Ad5[E1−,E2b−] vaccines, in contrast to Ad5[E1−] vaccines, do not promote activities that suppress innate immune signaling, thereby allowing for improved vaccine efficacy and a superior safety profile independently of previous Ad5 immunity. (asm.org)
  • The most advanced malaria vaccine, RTS,S, is comprised of an adjuvant portion of the Plasmodium falciparum circumsporozoite (CS) protein fused to and admixed with the hepatitis B virus surface antigen. (asm.org)
  • These results support the notion of first immunizations of infants with an adjuvanted CS protein vaccine, followed by a booster Ad35.CS/Ad26.CS vaccine at a later age, to induce lasting protection against malaria for which the Th1 response and immune memory is required. (asm.org)
  • Figure 1(see PDF version for figures) is a schematic drawing of the influenza virus showing the primary target the vaccine industry has focused on for the last 60 years, the hemagglutinin (HA) protein (spikes). (nae.edu)
  • In this study, we used umbilical cord blood (UCB)-derived dendritic cells (DCs) infected with a recombinant adenovirus encoding the livin gene as a vaccine to activate effector cells such as cytotoxic T lymphocytes (CTLs) to recognize and kill livin-expressing cancer cells in vitro as an improved strategy for overcoming the ability of these cancer cells to escape apoptosis and antitumor immune responses. (spandidos-publications.com)
  • Extended evaluation of a Phase 1/2 trial on dosing, safety, immunogenicity, and overall survival after immunizations with an advanced generation Ad5 [E1-, E2b-]-CEA(6D) vaccine in late stage colorectal cancer. (etubics.com)
  • We have applied this to an adenovirus (ad)-based vaccine encoding structural proteins (E3-E2-6K) of Venezuelan equine encephalitis virus (VEEV). (biomedcentral.com)
  • Furthermore, although both excellent booster vaccinations and raises in the vaccine dosage restored the antibody response towards the transgene item in preimmune rodents, human beings are expected to come across the normal serotypes of human being adenoviruses more often. (aksumuniversity.org)
  • We have constructed a novel oncolytic adenovirus, Ad5-gfa2(B)3-E1, for treatment of these tumors. (eur.nl)
  • The purpose of this study was to assess the impact of anti-adenovirus neutralizing antibodies (AdNAbs) on the distribution, tolerability, and efficacy of intravenously administered oncolytic adenovirus. (aacrjournals.org)
  • A translational model was developed to evaluate the impact of humoral immunity on intravenous administration of oncolytic adenovirus in humans. (aacrjournals.org)
  • Our results suggest that a majority of patients with preexisting adenovirus immunity would be candidates for intravenous administration of oncolytic adenovirus. (aacrjournals.org)
  • In this study, we developed a model to test whether or not preexisting humoral immunity representative of that found in the general population would be sufficient to neutralize the antitumor efficacy of a replicating oncolytic adenovirus. (aacrjournals.org)
  • We wished to model the effect of preexisting humoral immunity to adenovirus on antitumor efficacy of an oncolytic adenovirus. (aacrjournals.org)
  • A human tumor xenograft nude mouse model was developed to evaluate the inhibitory effect of human AdNAbs on the antitumor efficacy of an oncolytic adenovirus administered by the intravenous route. (aacrjournals.org)
  • Adenovirus, a double-stranded DNA virus belonging to the family Adenoviridae , is a possible candidate as an index virus. (asm.org)
  • Adenovirus vectors are developed from double stranded DNA viruses that belong to the Adenoviridae family. (oxfordgenetics.com)
  • Toxicity and immunity associated with adenovirus backbone gene expression is an important hurdle to overcome for successful gene therapy. (asm.org)
  • Studies carried out in vitro to evaluate adenovirus vectors with double deletions consistently feature an absence of detectable replication and late gene expression ( 1 , 17 ). (asm.org)
  • This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. (wikipedia.org)
  • The OR3-GFP expression cassette (light gray) is upstream of the Anch sequence consisting of 10 nucleation seeds for binding and oligomerization of the OR3-GFP protein (green spheres). (asm.org)
  • We found that disruption of signaling through the Raf-MEK-ERK pathway by inhibition of MEK up-regulated CAR expression, which was accompanied by increased representation of the protein at the cell surface. (aacrjournals.org)
  • We conclude that loss of CAR expression in cancer cells is, at least in part, mediated through the Raf-MEK-ERK signal transduction pathway and that pharmacological restoration of CAR at the cell surface could improve adenovirus-based treatments of cancer. (aacrjournals.org)
  • Loss of CAR expression on the cell surface significantly reduces infectivity, which can be rescued by ectopic expression of the protein. (aacrjournals.org)
  • 4 Despite the importance of CAR for adenovirus-based cancer therapies, information regarding molecular mechanisms regulating CAR expression in normal and malignant cells is not available. (aacrjournals.org)
  • In this study we investigated the effect of signaling through the Raf-MEK-ERK pathway on CAR expression in cell line models of human cancers (pancreatic and colorectal) that are potential targets for adenovirus-based therapies. (aacrjournals.org)
  • We show that pharmacological signaling through the Raf-MEK-ERK pathway up-regulates CAR expression on the cell surface of cancer cells, leading to increased uptake of adenovirus and cell killing. (aacrjournals.org)
  • What is false about adenovirus expression vectors? (brainscape.com)
  • The high levels of p53 present in adenovirus early region 1-transformed human cells do not cause up-regulation of MDM2 expression. (nih.gov)
  • In view of these observations the expression of MDM2 in a panel of adenovirus transformed human cell lines has been examined. (nih.gov)
  • While there was little variation in levels of 98K protein between cell lines, appreciable differences in the expression of the 80K component were apparent. (nih.gov)
  • There was no correlation between MDM2 and p53 expression in any of the adenovirus transformants, nor with the viral proteins expressed. (nih.gov)
  • p53 transcriptional activity was similar in all of the cell lines examined and did not correlate with protein expression. (nih.gov)
  • It is concluded, on the basis of all of these data, that the high concentrations of p53 found in adenovirus transformants are not transcriptionally active and have no influence on MDM2 expression. (nih.gov)
  • Viral transcription units are conventionally referred to as early (E1, E2, E3, and E4) and late, depending on their temporal expression relative to the onset of viral DNA replication ( 6 ). (pnas.org)
  • Adenovirus‑mediated recombinant BMP7 expression enhanced osteogenesis‑associated gene expression, calcium deposition, and alkaline phosphatase activity. (spandidos-publications.com)
  • Embodiments of the present invention provide methods for targeted inactivation of viral genomes using engineered zinc-finger proteins containing nuclease domains (ZFN), and targeted inactivation of viral gene expression using RNA interference. (patentgenius.com)
  • Finally, restoring Smad3 in rottlerin-treated A10 and PKCδ null cells rescues the ability of TGFβ to upregulate FN protein and mRNA expression. (ahajournals.org)
  • In particular, the novel PKC isoform, protein kinase C delta (PKCδ), is specifically activated by TGFβ in mesangial cells and modulates their expression of collagen via interaction with the Smad pathway. (ahajournals.org)
  • However, the mechanism by which TGFβ regulates gene expression of the matrix protein fibronectin remains in question. (ahajournals.org)
  • The backbone for our ViraPower™ adenoviral expression vectors is human adenovirus type 5 (Ad5). (thermofisher.com)
  • The value of the current understanding of the hematopoietic system to patient care is perhaps best illustrated in the field of malignancy where gene and protein expression profiles permit rapid and routine patient stratification. (bloodjournal.org)
  • Induce high-level transient protein expression. (signagen.com)
  • The first problem encountered was due to virus gene expression and leakiness in the replication defect of the commonly used defective adenovirus strains. (springer.com)
  • Bridge E, Ketner G (1989) Redundant control of adenovirus late gene expression by early region 4. (springer.com)
  • allows high expression of the recombinant protein. (vectorbiolabs.com)
  • Follistatin (FS) is the specific binding protein of activin and expression of both factors is regulated by inflammatory agents. (statescale.tk)
  • Another possibility is the presence of E1-like proteins inside the cell, compensating for the lack of adenoviral E1 expression in nonmelanoma cells. (barnardhealth.us)
  • 3) Targeted inhibition of gene expression Block expression of mutant gene at the levels of DNA, RNA or protein. (scribd.com)
  • Cardiac myocyte and fibroblast culture was performed to assess Ang-II and collagen protein expression before and after ACE2 gene transfection. (onlinejacc.org)
  • Results Four weeks after ACE2 gene transfer, the adenovirus-ACE2 group showed increased ACE2 expression, matrix metalloproteinase-2 activity, and LV ejection fractions and decreased LV volumes, myocardial fibrosis, and ACE, Ang-II, and collagen expression in comparison with the adenovirus-enhanced green fluorescent protein and control groups. (onlinejacc.org)
  • Objective: To analyze the correlation between connexin 43 (Cx43) and the expression of P16 and P21, aging-related proteins, and to investigate the possible role of Cx43 in the development of cell senescence with an aging model prepared by D-galactose (D-gal) intervention in the vascular smooth muscle cells (VSMCs) of guinea pig spiral modiolar artery (SMA). (bvsalud.org)
  • It is not known whether AP-1 proteins are sufficient to induce phenotypic changes or whether they must act in concert with other effectors. (ahajournals.org)
  • These results demonstrate that Ad5 [E1-, E2b-]-CEA can induce CMI immune responses which result in tumor growth inhibition despite the presence of pre-existing Ad5 immunity. (springer.com)
  • Other reasons for these failures include the genetic variability of the viral envelope proteins, which allows the virus to escape neutralizing antibodies, and the difficulty in identifying immunogens and immunization platforms that consistently induce antibodies that can neutralize several HIV clades ( 6 ). (rupress.org)
  • Much concern has focused on the direct toxic effects of adenoviruses, particularly as intravenous administration of the virus can induce acute liver injury, as shown in animal models. (bmj.com)
  • Numerous growth factors have been utilized in attempts to induce therapeutic angiogenesis by gene transfer or as recombinant proteins, with mixed results in clinical trials to date. (ahajournals.org)
  • Using a plasmid DNA encoding the small and the middle HBV envelope protein, we were able to generate anti-HBs antibodies in these transgenic mice, which in turn induce the clearance of circulating HBsAg. (bmj.com)
  • PRRSV contamination can induce significant and specific antibody and B-cell responses to a variety of PRRSV protein [8], [9]. (aksumuniversity.org)
  • Ad5 immune mice bearing CEA-expressing tumors that were treated with Ad5 [E1-, E2b-]-CEA had increased anti-tumor response as compared with Ad5 [E1-]-CEA treated mice. (springer.com)
  • When tested in human tumor xenografts in nude mice, Ad5-gfa2(B)3-E1 effectively suppressed the growth of GFAP-positive SNB-19 glial tumors but not of GFAP-negative A549 lung tumors. (eur.nl)
  • Major capsid proteins are indicated on the right. (asm.org)
  • Fourth and importantly, in productive infections, host cells are lysed and release their progeny, but the viruses are usually eliminated in a few days by antibodies to the capsid proteins. (aacrjournals.org)
  • But coronaviruses have an oily lipid membrane around them, which they acquire when they bud out from cells, whereas adenoviruses don't have one of these membranes - instead they have a protein "shell" called a capsid. (thebumblingbiochemist.com)
  • In this review we will consider the nature of adenovirus toxicity and the development of improved adenovirus vectors. (bmj.com)
  • 47. The recombinant virus of claim 46, wherein the recombinant virus is a recombinant adenovirus or a recombinant adeno-associated virus. (patentsencyclopedia.com)
  • 50. The method of claim 49, wherein the polynucleotide is a recombinant adenovirus or a recombinant adeno-associated virus. (patentsencyclopedia.com)
  • 53. The method of claim 52, wherein the polynucleotide is a recombinant adenovirus or a recombinant adeno-associated virus. (patentsencyclopedia.com)
  • Cellular immunity to viral antigens limits E1-deleted adenoviruses for gene therapy. (nii.ac.jp)
  • A system of genetically determined antigens (proteins) located on the surface of the erythrocyte. (hepatitiscentral.com)
  • Data obtained from murine and other animal models have shown that host immune responses to viral and transgene protein products are responsible for eliminating transduced cells and preventing readministration ( 9 , 20-22 , 41-44 ). (asm.org)
  • 9 . The isolated protein of claim 7 wherein said protein is a functional equivalent of said EDG1 protein and inhibits proliferation of MCF7 cells. (google.co.uk)
  • 16 The adenoviruses were plaque-purified, amplified, and titered in 293 cells. (ahajournals.org)
  • In addition, there are methods to study transcription and splicing with in vitro systems and for the adenovirus-mediated transformation of cells to a malignant state. (springer.com)
  • The higher viral protein content in HDV-producing cells was also consistent with an increased activation of autophagy and cell death, in which earlier cell death compromised volumetric productivity. (biomedsearch.com)
  • Large quantities of both adenoviruses are then produced by HEK 293 cells that have the E1 gene necessary for viral replication. (wikipedia.org)
  • This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. (wikipedia.org)
  • Survival Motor Neuron Protein is Released from Cells in Exosomes: A Potential Biomarker for Spinal Muscular Atrophy. (nih.gov)
  • Using cultures of human peripheral blood mononuclear cells (hPBMCs) derived from multiple human donors, we found that Ad5[E1−,E2b−] vaccines trigger higher levels of hPBMC proinflammatory cytokine secretion than Ad5[E1−] vaccines. (asm.org)
  • After Raf-MEK-ERK inhibition, adenovirus entry into cells was increased and cell killing by replication competent adenoviruses was enhanced in a CAR-dependent manner. (aacrjournals.org)
  • Cell viability assays showed efficient elimination of GFAP-positive cells by Ad5-gfa2(B)3-E1, in some cell lines as efficiently as wtAd5, while the elimination was attenuated in GFAP-negative cell lines. (eur.nl)
  • Gelonin, a New Inhibitor of Protein Synthesis, Nontoxic to Intact Cells," Journal of Biological Chemistry 255(14): 6947-6953, 1980. (patentgenius.com)
  • We and others have previously shown that p53 is present at high levels in adenovirus-transformed cells which express the larger E1B protein. (nih.gov)
  • Stable interactions were observed between p53 and MDM2 in the adenovirus-transformed cell lines and in Ad5 E1 HEK 293 cells a ternary complex of p53, MDM2, and the Ad5 E1B 58K protein was demonstrated. (nih.gov)
  • In view of the lack of correlation between the level of p53 and MDM2 in adenovirus E1-transformed cells, the capacity of p53 to cause transcriptional activation was assessed using transfected CAT constructs linked to p53 responsive elements. (nih.gov)
  • The second and more widely used method involves homologous recombination in mammalian cells capable of complementing defective adenoviruses ("packaging lines") ( 9 , 10 ). (pnas.org)
  • In the present study, human dermal‑derived CD105+ fibroblast cells (CD105+ hDDFCs) were isolated from human foreskin specimens using immunomagnetic isolation methods to examine the role of bone morphogenetic protein (BMP)‑7 in osteogenic differentiation. (spandidos-publications.com)
  • The Generx FGF-4 transgene has been engineered to include a signal peptide, which enables effective secretion from cells that express the protein (such as cardiac myocytes). (medindia.net)
  • Generx is distributed into the microvascular pathways of the heart, and transfects cardiac cells by binding to cell surface coxsackievirus-adenovirus receptors (CAR). (medindia.net)
  • Several companies (Novavax and Protein Sciences are two of the main players) are working on using insect cells as vectors for making HA protein. (nae.edu)
  • We are currently employing proteomic techniques to identify novel adenovirus early region-binding proteins in adenovirus-infected and adenovirus-transformed cells. (birmingham.ac.uk)
  • The anaphase-promoting complex/cyclosome (APC/C) is a multicomponent E3 ubiquitin ligase that, by targeting protein substrates for 26S proteasome-mediated degradation through ubiquitylation, coordinates the temporal progression of eukaryotic cells through mitosis and the subsequent G1 phase of the cell cycle. (birmingham.ac.uk)
  • Oncolytic adenoviruses are potent chemosensitizers, particularly in cells with functional p53 ( 1 ), and have shown encouraging antineoplastic activity in clinical trials in combination with chemotherapy ( 2 , 3 ). (aacrjournals.org)
  • Methods and Results- TGFβ1 elicited a time-dependent induction of FN protein and mRNA in A10 rat aortic smooth muscle cells (SMCs). (ahajournals.org)
  • Furthermore, we found that Smad3 protein and mRNA were markedly reduced in AdPKCδ DN-treated A10 cells and in PKCδ null cells. (ahajournals.org)
  • Baatz JE, Bruno MD, Ciraolo PJ, Glasser SW, Stripp BR, Smyth KL, Korfhagen TR (1994) Utilization of modified surfactant-associated protein B for delivery of DNA to airway cells in culture. (springer.com)
  • Bai M, Harfe B, Freimuth P (1993) Mutations that alter an Arg-Gly-Asp (RGD) sequence in the adenovirus type 2 penton base protein abolish its cell rounding activity and delay virus reproduction in flat cells. (springer.com)
  • Chen P, Omelles D, Shenk T (1993) The adenovirus L3 23-kilodalton proteinase cleaves the armino-terminal head domain from cytokeratin 18 and disrupts the cytokeratin network of HeLa cells. (springer.com)
  • Cotten M, Baker A, Saltik M, Wagner E, Buschle M (1994a) Lipopolysaccharide is a frequent contaminant of plasmid DNA preparations and can be toxic to primary cells in the presence of adenovirus. (springer.com)
  • We have generated novel conditionally replicative adenoviruses (CRAds) targeted to melanoma cells. (aacrjournals.org)
  • The properties of these CRAds were compared with wild-type adenovirus (Adwt) and our previous CRAd with a targeted E1A CRII mutation (AdTyrΔ24) in submerged cultures of melanoma cells and nonmelanoma control cells. (aacrjournals.org)
  • The very first viral gene products synthesized after cells are infected with adenovirus. (bvsalud.org)
  • Most of the E3 proteins are involved in modulating the immune response of infected cells. (vectorbiolabs.com)
  • There are many advantages to using an adenovirus to introduce genetic material into host cells. (vectorbiolabs.com)
  • Mammalian cells, i.e., baby hamster kidney 21 Sotrastaurin (BHK-21) cells, E1-transfected 293 cells, thymidine kinase-negative (TK? (aksumuniversity.org)
  • As compared to retroviruses, adenovirus are capable of infecting a broader variety of cells, including those that have a comparatively slow rate of cell division, such as lungs cells. (oxfordgenetics.com)
  • Adenovirus vectors have a high transduction efficiency for dividing and non-dividing cells. (oxfordgenetics.com)
  • If you could get a virus to deliver those recipes, you could get the cells to make those proteins you wanted. (thebumblingbiochemist.com)
  • So in order to produce it, manufacturers infect cells in a dish/flask that already have a copy of the E1 gene. (thebumblingbiochemist.com)
  • Replication of the adenovirus inside these cells will eventually lead to cell lysis, thereby releasing newly formed virions within the tumor mass. (barnardhealth.us)
  • Porcine adenoviral vectors evade preexisting humoral immunity to adenoviruses and efficiently infect both human and murine cells in culture. (microbiologyresearch.org)
  • Nuclear factor I is specifically targeted to discrete subnuclear sites in adenovirus type 2-infected cells. (microbiologyresearch.org)
  • Adenoviruses (Ads) are double-stranded DNA viruses, whereas coronaviruses are single-stranded RNA viruses. (thebumblingbiochemist.com)
  • When adenoviruses infect a host cell, their genetic material (DNA) is inserted into the host cell. (oxfordgenetics.com)
  • In one embodiment, zinc-finger proteins in which DNA binding sites are altered such that they recognize and bind different, desired DNA sequences contained in hepatitis B virus (HBV) and that include nuclease domains are used for inactivation. (patentgenius.com)
  • The partial amino-terminal sequences of E2a and E2b were identical and different from that of E1. (microbiologyresearch.org)
  • Belin M.-T, Boulanger P (1993) Involvement of cellular adhesion sequences in the attachment of adenovirus to the HeLa cell surface. (springer.com)
  • Material and methods: An 8-mer solid- phase (TG resin) library was screened with a neutralising and protective RSV fusion protein specific monoclonal antibodies (Mab-19). (tudelft.nl)
  • Antibodies, which are initially of the IgM then IgG isotype (predominantly IgG2a), recognise several of the B cell epitopes present on the S, preS2, or preS1 domains of the envelope proteins. (bmj.com)
  • The presence of serum anti-makorin 1 (MKRN1) antibodies (s-MKRN1-Abs) was examined by Western blotting using bacterially expressed MKRN1 protein. (statescale.tk)
  • Irvin, "Purification and Partial Characterization of the Antiviral Protein from Phytolacca americana Which Inhibits Eukaryotic Protein Synthesis," Archives of Biochemistry and Biophysics 169: 522-528, 1975. (patentgenius.com)
  • Purification and partial characterization of another form of the antiviral protein from the seeds of Phytolacca americana L. (pokeweed)," Biochem. (patentgenius.com)
  • A similar titer reconstituted in the nude mice with human serum, as was done in the SCID/beige mice, did not abrogate the antitumor efficacy of the replicating adenovirus after intravenous administration. (aacrjournals.org)
  • the same titer had no affect on the activity of an equivalent dose of replicating adenovirus. (aacrjournals.org)
  • The E1 gene products, including E1A and E1B, are involved in replication of the virus. (vectorbiolabs.com)
  • The E4 gene products are involved in the metabolism of virus messenger RNA and provide functions that promote virus DNA replication and shut-off of host protein synthesis. (vectorbiolabs.com)
  • E4 gene products are involved in the metabolism of virus messenger RNA (mRNA), promotion of viral DNA replication and in shutting-off of host protein synthesis. (oxfordgenetics.com)
  • This experience points to the limitations of animal studies in predicting human responses, the steep toxicity curve for replication defective adenovirus vectors, substantial subject-to-subject variation in host responses to systemically administered vectors, and the need for further study of the immune response to these vectors. (nih.gov)
  • 11 . A polypeptide which comprises a contiguous sequence within SEQ ID NO. 2, wherein said contiguous sequence is at least 8 amino acids in length, and wherein said polypeptide is a functional equivalent of human EDG1 protein. (google.co.uk)
  • 15 . An antibody which binds to one or more epitopes in human EDG1 protein, wherein said EDG1 protein comprises SEQ ID NO. 2. (google.co.uk)
  • However, the regulatory effects of AP-1 proteins in human ECs remain unclear. (ahajournals.org)
  • Using an adenovirus-mediated gene transfer protocol, we found that the AP-1 proteins c-Fos and c-Jun directly cause phenotypic activation and induction of intercellular adhesion molecule-1 (ICAM-1) and monocyte chemoattractant protein-1 (MCP-1) in human ECs. (ahajournals.org)
  • A 34 amino acid synthetic analog of human parathyroid hormone-related protein (PTHrP) (PTHrP(1-34) analog), with bone-growing and bone density conserving activities. (cancer.gov)
  • A soluble fusion protein consisting of the extracellular domain of human cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) linked to a modified Fc (hinge, CH2, and CH3 domains) portion of human immunoglobulin G1 (IgG1) with immunosuppressive activity. (cancer.gov)
  • Evaluation of Cross-Reactive Humoral and Cell-Mediated Immune Responses among Human, Bovine and Porcine Adenoviruses. (purdue.edu)
  • Bovine adenovirus type 3 internalization is independent of primary receptors of human adenovirus type 5 and porcine adenovirus type 3. (purdue.edu)
  • Even in the presence of anti-Ad5 immunity, recent murine and human studies have confirmed E2b gene-deleted Ad5 (Ad5[E1−,E2b−]) vaccines to be highly efficacious inducers of transgene-specific memory responses and significantly less toxic options than Ad5[E1−] vaccines. (asm.org)
  • To this end, a human tumor xenograft nude mouse model was developed to model preexisting humoral immunity to adenovirus observed in the human population. (aacrjournals.org)
  • Real-time PCR was applied to quantify the abundance of human adenoviruses in two southern California urban rivers, the San Gabriel and Los Angeles. (asm.org)
  • A total of 114 river samples from five different locations were collected over a 1-year period and analyzed for human adenoviruses, along with fecal indicator bacteria and coliphages. (asm.org)
  • However, a plaque assay using two human tissue culture cell lines, HEK-293A and A549, yielded negative results, suggesting that adenoviruses detected by real-time PCR are likely noninfectious. (asm.org)
  • However, no significant correlations were found between human adenoviruses quantified by real-time PCR and culturable coliphages or fecal indicator bacteria. (asm.org)
  • This study presents the first quantitative measurement of human adenovirus genomes in urban rivers and their statistical relationship to fecal indicator bacteria and coliphages. (asm.org)
  • Adenovirus is frequently found in urban rivers and polluted coastal waters ( 5 , 7 , 20 , 32 , 33 , 39 ), and studies conducted in Europe suggest that adenovirus be used as an index of human viral pollution ( 32 ). (asm.org)
  • 4) at the pentapeptide level, the viral-versus-human overlap is extensive (14,227 matches), with the viral pentapeptides disseminated throughout 10,312 human proteins. (bioscience.org)
  • Specifically, the current study addressed the following questions: i) How many human proteins harbor HIV peptide modules? (bioscience.org)
  • In human malignant neuroblasts, p53 protein is wild-type but known to be excluded from the nucleus, therefore disabling its function as a tumor suppressor. (biologists.org)
  • No, our system uses human adenovirus type 5 (Ad5). (thermofisher.com)
  • Wild-type adenoviruses, including human adenovirus type 5, are associated with a number of mild disorders, such as respiratory infections in the elderly or children. (vectorbiolabs.com)
  • Interaction of the putative human cytomegalovirus portal protein pUL104 with the large terminase subunit pUL56 and its inhibition by benzimidazole-D-ribonucleosides. (microbiologyresearch.org)
  • Livin is a novel member of the inhibitors of apoptosis (IAP) protein family that inhibits cell apoptosis. (spandidos-publications.com)
  • The inhibitors of apoptosis proteins (IAPs) inhibit apoptosis by interacting with the pro-apoptotic caspases via their baculoviral IAP repeat (BIR) ( 3 ). (spandidos-publications.com)
  • A novel assay that integrates measurements of blood-borne host-proteins (tumor necrosis factor-related apoptosis-inducing ligand, interferon γ-induced protein-10, and C-reactive protein [CRP]) was developed to assist in differentiation between bacterial and viral disease. (aappublications.org)
  • However, several factors can influence the optimal MOI including the nature of your mammalian cell line (non-dividing vs. dividing), transduction efficiency, your application of interest, and your protein of interest. (thermofisher.com)
  • 6 It is an adaptor protein and E3 ubiquitin-protein ligase that can either positively or negatively regulate signal transduction cascades. (ahajournals.org)
  • 3 . An isolated polynucleotide comprising a sequence which encodes EDG1 protein or a functional equivalent thereof, wherein said EDG1 protein comprises SEQ ID NO. 2, and wherein said functional equivalent comprises a sequence which is at least 85% identical to SEQ ID NO. 2. (google.co.uk)
  • Work in our lab established that the APC/C subunits APC5 and APC7 possess within their primary sequence CBP and p300 protein-protein interaction domains that are evolutionarily conserved in adenovirus E1A (Turnell et al . (birmingham.ac.uk)
  • The individual proteins were subjected to amino-terminal sequence analysis by Edman degradation, carboxyl-terminal structure analysis by digestion with carboxypeptidases and quantitative amino acid composition analysis. (microbiologyresearch.org)
  • First generation adenoviral vectors were developed by replacing the gene sequence at the E1 region with the therapeutic gene. (oxfordgenetics.com)
  • Ternary complex formation on the adenovirus packaging sequence by the IVa2 and L4 22-kilodalton proteins. (microbiologyresearch.org)
  • Effects of mutations in the adenoviral E1B 55-kilodalton protein coding sequence on viral late mRNA metabolism. (microbiologyresearch.org)
  • 44. The fusion protein of claim 40, comprising the amino acid sequence of FIG. 5B (SEQ ID NO:6) or FIG. 6B (SEQ ID NO:8), or an active variant thereof having at least 90% sequence identity to the sequence of FIG. 5B (SEQ ID NO:6) or FIG. 6B (SEQ ID NO:8). (patentsencyclopedia.com)
  • Disclosed is an isolated antigen binding protein, such as but not limited to, an antibody or antibody fragment. (patents.com)
  • The large T antigen encoded by SV40 or polyoma viruses inactivates several key proteins involved in cell cycle regulation ( DeCaprio, 1999 ). (embopress.org)
  • The viral proteins discussed are the two major KSHV encoded transcription factors, K-Rta (KSHV replication and transcriptional activator) and K-bZIP (KSHV basic leucine zipper) and the viral latency-associated nuclear antigen (LANA). (frontiersin.org)
  • Systemic immunisation of mice by intramuscular injection of plasmid DNA expressing HBV envelope proteins and HBV surface antigen (HBsAg) induces rapid, strong and sustained humoral and cell-mediated immune responses. (bmj.com)
  • Improved adenovirus packaging cell lines to support the growth of replication-defective gene-delivery vectors. (etubics.com)
  • Adenovirus type 5 expresses proteins that regulate the activity and stability of the tumor suppressor p53. (aacrjournals.org)
  • To assess whether different SIBLINGs regulate biomineralization in a similar manner, and how phosphorylation impacts their activity, we studied the effects of two SIBLINGs, dentin matrix protein 1 (DMP1) and dentin phosphophoryn (DPP), on mineral morphology and organization in vitro . (pubmedcentralcanada.ca)
  • also reviewed in reference 2 ), a strategy utilized to regulate several key aspects of host proteins ( 3 , 4 ). (asm.org)
  • He is particularly interested in how adenovirus oncoproteins modulate the function of host cell proteins to regulate chromatin, the ubiquitin-proteasome pathway and DNA damage pathways. (birmingham.ac.uk)
  • In vitro experiments indicated that GKRP was able to increase both GK protein and enzymatic activity levels, suggesting that another role for GKRP is to stabilize and/or protect GK. (diabetesjournals.org)
  • Therefore, modeling the effect of preexisting adenovirus immunity on systemic administration of therapeutic adenoviruses is challenging. (aacrjournals.org)
  • Q-PCR showed that Ad5-gfa2(B)3-E1 replicated 4.5 times more efficiently in the GFAP-positive than in the GFAP-negative cell lines. (eur.nl)
  • Adenovirus Methods and Protocols will be useful to both entry-level and senior scientists seeking to enter the adenovirus field, to researchers from other areas wishing to construct adenovirus vectors for their own research, and to adenovirologists wanting to enter new sectors of research. (springer.com)
  • the E3 region encodes proteins involved in evading host immunity and is dispensable for viral production per se . (pnas.org)
  • In the present study, we tested by the hypothesis that the Th1 immune response to CS protein, in particular the CD8 + T-cell response, which is needed for strong and lasting malaria immunity, is boosted to sustainable levels vectors adenovirus and 26 with an homologous insert 35 (Ad35.CS/Ad26.CS). (asm.org)
  • 6 , 8 , 11 - 13 This freedom of interaction is believed to be the structural basis of the multifunctionality of SIBLING proteins. (pubmedcentralcanada.ca)
  • Figure 2 shows a ribbon model of the HA protein, the structural feature of the influenza virus targeted by most influenza vaccines. (nae.edu)
  • The structural proteins E1, E2a, E2b and C of rubella virus (RV) were purified by preparative SDS-PAGE. (microbiologyresearch.org)
  • We have previously developed adenovirus (ad)-based vaccines which encode the structural proteins of VEEV. (biomedcentral.com)
  • The structural proteins of VEEV (core, E3, E2, 6K and E1) are initially translated from a 26S subgenomic RNA as a single polyprotein. (biomedcentral.com)
  • This molecule may also be involved in virion assembly or maturation, as some previously characterized fiber mutants were defective for processing of viral structural proteins. (scripps.edu)
  • Production was subsequently performed in an E1-, E2a-, and E4-complementing cell line. (asm.org)
  • In Adenovirus Methods and Protocols, William S.M. Wold has organized a collection of readily reproducible methods for conducting research with adenoviruses, the premier and most widely used model in cell and molecular biology. (springer.com)
  • In the β-cell, GK activity is largely regulated by transcriptional mechanisms ( 1 , 2 ), whereas in the liver, GK activity is also acutely regulated by its binding to the GK regulatory protein (GKRP), localized within the nucleus ( 11 , 12 , 13 ). (diabetesjournals.org)
  • The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. (wikipedia.org)
  • They require a cell line with helper proteins to assist packaging d. (brainscape.com)
  • After transfections of such plasmids into a mammalian packaging cell line, viral production is conveniently followed with the aid of green fluorescent protein, encoded by a gene incorporated into the viral backbone. (pnas.org)
  • HCV core protein has been reported to inhibit CD8 + T cell responses. (jimmunol.org)
  • The adenovirus (Ad) fiber protein largely determines viral tropism through interaction with specific cell surface receptors. (scripps.edu)
  • Even if the cell doesn't normally make that protein. (thebumblingbiochemist.com)
  • The generation of a functional protein product from the therapeutic gene restores the target cell to a normal state. (scribd.com)
  • 7,8,10 c-Cbl is expressed in virtually all cell types primarily as a cytosolic protein. (ahajournals.org)
  • Proteins encoded by adenoviruses that are synthesized prior to, and in the absence of, viral DNA replication. (curehunter.com)
  • Production of adenovirus vectors and their use as a delivery system for influenza vaccines. (purdue.edu)
  • however, widespread preexisting Ad5 immunity has been considered a developmental impediment to the use of traditional, or conventional, E1 and E3 gene-deleted Ad5 (Ad5[E1−]) vaccines. (asm.org)
  • Aims: To identify peptides that mimic (mimotopes) conformational and protective epitopes of RSV fusion protein and to assess their efficacy as immunogens and potential vaccines. (tudelft.nl)
  • Among the potential recombinant protein vaccines reviewed are HC, light chain-heavy chain translocation domain, and chemically or genetically inactivated holotoxin. (mdpi.com)
  • Final adenovirus yield may depend on the nature of transgene. (signagen.com)
  • We report that Ad5-immune mice immunized multiple times with Ad5 [E1-, E2b-]-CEA induced CEA-specific CMI responses that were significantly increased over those detected in Ad5-immune mice immunized multiple times with a current generation Ad5 [E1-]-CEA. (springer.com)
  • Papp Z, Babiuk LA, Baca-Estrada ME (1999) The effect of pre-existing adenovirus-specific immunity on immune responses induced by recombinant adenovirus expressing glycoprotein D of bovine herpesvirus type 1. (springer.com)
  • CAR 3 is a 46-kDa transmembrane protein that enables virus attachment via interaction with the adenovirus fiber-knob protein. (aacrjournals.org)
  • You J, Croyle JL, Nishimura A, Ozato K, Howley PM. Interaction of the bovine papillomavirus E2 protein with Brd4 tethers the viral DNA to host mitotic chromosomes. (harvard.edu)
  • The interaction between Ubc9 and most targets is not stable enough for efficient transfer, and therefore requires additional proteins, the so-called E3 ligases. (biomedcentral.com)
  • On stimulation, c-Cbl is rapidly phosphorylated on the tyrosine residues localized within its C terminus, leading to its interaction with a plethora of SH2-containing proteins such as the p85 subunit of phosphatidylinositol 3-kinase (PI3K), the Crk adaptor proteins, and Src family tyrosine kinases. (ahajournals.org)