Adenoviridae Infections: Virus diseases caused by the ADENOVIRIDAE.Adenoviridae: A family of non-enveloped viruses infecting mammals (MASTADENOVIRUS) and birds (AVIADENOVIRUS) or both (ATADENOVIRUS). Infections may be asymptomatic or result in a variety of diseases.Aviadenovirus: A genus of ADENOVIRIDAE that infects birds. The type species is FOWL ADENOVIRUS A.Atadenovirus: A genus of ADENOVIRIDAE that comprises viruses of several species of MAMMALS and BIRDS. The type species is Ovine adenovirus D.Adenoids: A collection of lymphoid nodules on the posterior wall and roof of the NASOPHARYNX.Mastadenovirus: A genus of ADENOVIRIDAE that infects MAMMALS including humans and causes a wide range of diseases. The type species is Human adenovirus C (see ADENOVIRUSES, HUMAN).Encyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)Common Cold: A catarrhal disorder of the upper respiratory tract, which may be viral or a mixed infection. It generally involves a runny nose, nasal congestion, and sneezing.GeeseAdenoviruses, Human: Species of the genus MASTADENOVIRUS, causing a wide range of diseases in humans. Infections are mostly asymptomatic, but can be associated with diseases of the respiratory, ocular, and gastrointestinal systems. Serotypes (named with Arabic numbers) have been grouped into species designated Human adenovirus A-F.Integrin alphaV: An alpha integrin with a molecular weight of 160-kDa that is found in a variety of cell types. It undergoes posttranslational cleavage into a heavy and a light chain that are connected by disulfide bonds. Integrin alphaV can combine with several different beta subunits to form heterodimers that generally bind to RGD sequence-containing extracellular matrix proteins.Coxsackie and Adenovirus Receptor-Like Membrane Protein: An Ig superfamily transmembrane protein that localizes to junctional complexes that occur between ENDOTHELIAL CELLS and EPTHELIAL CELLS. The protein may play a role in cell-cell adhesion and is the primary site for the attachment of ADENOVIRUSES during infection.Capsid Proteins: Proteins that form the CAPSID of VIRUSES.Integrins: A family of transmembrane glycoproteins (MEMBRANE GLYCOPROTEINS) consisting of noncovalent heterodimers. They interact with a wide variety of ligands including EXTRACELLULAR MATRIX PROTEINS; COMPLEMENT, and other cells, while their intracellular domains interact with the CYTOSKELETON. The integrins consist of at least three identified families: the cytoadhesin receptors(RECEPTORS, CYTOADHESIN), the leukocyte adhesion receptors (RECEPTORS, LEUKOCYTE ADHESION), and the VERY LATE ANTIGEN RECEPTORS. Each family contains a common beta-subunit (INTEGRIN BETA CHAINS) combined with one or more distinct alpha-subunits (INTEGRIN ALPHA CHAINS). These receptors participate in cell-matrix and cell-cell adhesion in many physiologically important processes, including embryological development; HEMOSTASIS; THROMBOSIS; WOUND HEALING; immune and nonimmune defense mechanisms; and oncogenic transformation.Dictionaries, MedicalDictionaries as Topic: Lists of words, usually in alphabetical order, giving information about form, pronunciation, etymology, grammar, and meaning.Dictionaries, ChemicalTerminology as Topic: The terms, expressions, designations, or symbols used in a particular science, discipline, or specialized subject area.Parainfluenza Virus 2, Human: A species of RUBULAVIRUS associated particularly with acute laryngotracheitis (CROUP) in children aged 6 months to 3 years.Parainfluenza Virus 3, Human: A species of RESPIROVIRUS frequently isolated from small children with pharyngitis, bronchitis, and pneumonia.Respirovirus: A genus of the family PARAMYXOVIRIDAE (subfamily PARAMYXOVIRINAE) where all the virions have both HEMAGGLUTININ and NEURAMINIDASE activities and encode a non-structural C protein. SENDAI VIRUS is the type species.Parainfluenza Virus 1, Human: A species of RESPIROVIRUS also called hemadsorption virus 2 (HA2), which causes laryngotracheitis in humans, especially children.Endogenous Retroviruses: Retroviruses that have integrated into the germline (PROVIRUSES) that have lost infectious capability but retained the capability to transpose.Retroviridae: Family of RNA viruses that infects birds and mammals and encodes the enzyme reverse transcriptase. The family contains seven genera: DELTARETROVIRUS; LENTIVIRUS; RETROVIRUSES TYPE B, MAMMALIAN; ALPHARETROVIRUS; GAMMARETROVIRUS; RETROVIRUSES TYPE D; and SPUMAVIRUS. A key feature of retrovirus biology is the synthesis of a DNA copy of the genome which is integrated into cellular DNA. After integration it is sometimes not expressed but maintained in a latent state (PROVIRUSES).Retroviridae Infections: Virus diseases caused by the RETROVIRIDAE.Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Terminal Repeat Sequences: Nucleotide sequences repeated on both the 5' and 3' ends of a sequence under consideration. For example, the hallmarks of a transposon are that it is flanked by inverted repeats on each end and the inverted repeats are flanked by direct repeats. The Delta element of Ty retrotransposons and LTRs (long terminal repeats) are examples of this concept.Multiple Sclerosis: An autoimmune disorder mainly affecting young adults and characterized by destruction of myelin in the central nervous system. Pathologic findings include multiple sharply demarcated areas of demyelination throughout the white matter of the central nervous system. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia, and bladder dysfunction. The usual pattern is one of recurrent attacks followed by partial recovery (see MULTIPLE SCLEROSIS, RELAPSING-REMITTING), but acute fulminating and chronic progressive forms (see MULTIPLE SCLEROSIS, CHRONIC PROGRESSIVE) also occur. (Adams et al., Principles of Neurology, 6th ed, p903)Patents as Topic: Exclusive legal rights or privileges applied to inventions, plants, etc.Nucleic Acids: High molecular weight polymers containing a mixture of purine and pyrimidine nucleotides chained together by ribose or deoxyribose linkages.Nucleic Acid Hybridization: Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)Illusions: The misinterpretation of a real external, sensory experience.DNA Probes: Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.DNA: A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).Oligonucleotide Probes: Synthetic or natural oligonucleotides used in hybridization studies in order to identify and study specific nucleic acid fragments, e.g., DNA segments near or within a specific gene locus or gene. The probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the probe include the radioisotope labels 32P and 125I and the chemical label biotin.GermanyRed Cross: International collective of humanitarian organizations led by volunteers and guided by its Congressional Charter and the Fundamental Principles of the International Red Cross Movement, to provide relief to victims of disaster and help people prevent, prepare for, and respond to emergencies.Classification: The systematic arrangement of entities in any field into categories classes based on common characteristics such as properties, morphology, subject matter, etc.Bacteria: One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.Editorial Policies: The guidelines and policy statements set forth by the editor(s) or editorial board of a publication.Indigo Carmine: Indolesulfonic acid used as a dye in renal function testing for the detection of nitrates and chlorates, and in the testing of milk.BooksVanilla: A plant genus of the family ORCHIDACEAE that is the source of the familiar flavoring used in foods and medicines (FLAVORING AGENTS).Paeonia: A plant genus of the family Paeoniaceae, order Dilleniales, subclass Dilleniidae, class Magnoliopsida. These perennial herbs are up to 2 m (6') tall. Leaves are alternate and are divided into three lobes, each lobe being further divided into three smaller lobes. The large flowers are symmetrical, bisexual, have 5 sepals, 5 petals (sometimes 10), and many stamens.Gabon: A republic in west equatorial Africa, south of CAMEROON and west of the CONGO. Its capital is Libreville.Filoviridae: A family of RNA viruses, of the order MONONEGAVIRALES, containing filamentous virions. Although they resemble RHABDOVIRIDAE in possessing helical nucleocapsids, Filoviridae differ in the length and degree of branching in their virions. There are two genera: EBOLAVIRUS and MARBURGVIRUS.Africa, Central: The geographical area of Africa comprising CAMEROON; CENTRAL AFRICAN REPUBLIC; CHAD; CONGO; EQUATORIAL GUINEA; GABON; and DEMOCRATIC REPUBLIC OF THE CONGO.Filoviridae Infections: Infections with viruses of the family FILOVIRIDAE. The infections in humans consist of a variety of clinically similar viral hemorrhagic fevers but the natural reservoir host is unknown.BangladeshHemorrhagic Fever, Ebola: A highly fatal, acute hemorrhagic fever, clinically very similar to MARBURG VIRUS DISEASE, caused by EBOLAVIRUS, first occurring in the Sudan and adjacent northwestern (what was then) Zaire.Africa

Adenoviral gene transfer into the normal and injured spinal cord: enhanced transgene stability by combined administration of temperature-sensitive virus and transient immune blockade. (1/9136)

This study characterized gene transfer into both normal and injured adult rat dorsal spinal cord using first (E1-/E3-) or second (E1-/E2A125/E3-, temperature-sensitive; ts) generation of replication-defective adenoviral (Ad) vectors. A novel immunosuppressive regimen aimed at blocking CD4/CD45 lymphocytic receptors was tested for improving transgene persistence. In addition, the effect of gene transfer on nociception was also evaluated. Seven days after treatment, numerous LacZ-positive cells were observed after transfection with either viral vector. By 21 days after transfection, beta-galactosidase staining was reduced and suggestive of ongoing cytopathology in both Ad-treated groups, despite the fact that the immunogenicity of LacZ/Adts appeared less when compared with that elicited by the LacZ/Ad vector. In contrast, immunosuppressed animals showed a significant (P < or = 0.05) increase in the number of LacZ-positive cells not displaying cytopathology. In these animals, a concomitant reduction in numbers of macrophages/microglia and CD4 and CD8 lymphocytes was observed. Only animals that received LacZ/Adts and immunosuppression showed transgene expression after 60 days. Similar results were observed in animals in which the L4-L5 dorsal roots were lesioned before transfection. Gene transfer into the dorsal spinal cord did not affect nociception, independent of the adenovirus vector. These results indicate that immune blockade of the CD4/CD45 lymphocytic receptors enhanced transgene stability in adult animals with normal or injured spinal cords and that persistent transgene expression in the spinal cord does not interfere with normal neural function.  (+info)

Definition of a major p53 binding site on Ad2E1B58K protein and a possible nuclear localization signal on the Ad12E1B54K protein. (2/9136)

Previous studies have established that adenovirus 2/5 early region 1B (Ad E1B) 58K protein binds p53 strongly and co-localizes with it to cytoplasmic dense bodies whilst the homologous Ad12E1B54K protein binds only weakly and co-localizes primarily to the nucleus in Ad12E1 transformed cells. We have used these properties of the E1B proteins from different viral serotypes to map the p53 binding site on the Ad2/5 protein. A set of chimaeric genes was constructed containing different proportions of the Ad12 and Ad2E1B DNA. These, together with Ad12E1A and E1B19K DNA, were transfected into baby rat kidney cells and transformed lines isolated. From an examination of the properties of these Ad12/Ad2E1B fusion proteins in co-immunoprecipitation and subcellular localization experiments it has been concluded that the p53 binding site on Ad2E1B58K protein lies between amino acids 216 and 235 and that the homologous region on Ad12E1B54K protein also binds p53. In addition, a unique nuclear localization signal is located on Ad12E1B54K between residues 228 and 239. We suggest that primary structure differences in these regions of the Ad2 and Ad12E1B proteins are responsible for the different subcellular localizations in AdE1 transformants.  (+info)

Inducible genetic suppression of neuronal excitability. (3/9136)

Graded, reversible suppression of neuronal excitability represents a logical goal of therapy for epilepsy and intractable pain. To achieve such suppression, we have developed the means to transfer "electrical silencing" genes into neurons with sensitive control of transgene expression. An ecdysone-inducible promoter drives the expression of inwardly rectifying potassium channels in polycistronic adenoviral vectors. Infection of superior cervical ganglion neurons did not affect normal electrical activity but suppressed excitability after the induction of gene expression. These experiments demonstrate the feasibility of controlled ion channel expression after somatic gene transfer into neurons and serve as the prototype for a novel generalizable approach to modulate excitability.  (+info)

Reversal of hyperlipidaemia in apolipoprotein C1 transgenic mice by adenovirus-mediated gene delivery of the low-density-lipoprotein receptor, but not by the very-low-density-lipoprotein receptor. (4/9136)

We have shown previously that human apolipoprotein (apo)C1 transgenic mice exhibit hyperlipidaemia, due primarily to an impaired clearance of very-low-density lipoprotein (VLDL) particles from the circulation. In the absence of at least the low-density-lipoprotein receptor (LDLR), it was shown that APOC1 overexpression in transgenic mice inhibited the hepatic uptake of VLDL via the LDLR-related protein. In the present study, we have now examined the effect of apoC1 on the binding of lipoproteins to both the VLDL receptor (VLDLR) and the LDLR. The binding specificity of the VLDLR and LDLR for apoC1-enriched lipoprotein particles was examined in vivo through adenovirus-mediated gene transfer of the VLDLR and the LDLR [giving rise to adenovirus-containing (Ad)-VLDLR and Ad-LDLR respectively] in APOC1 transgenic mice, LDLR-deficient (LDLR-/-) mice and wild-type mice. Remarkably, Ad-VLDLR treatment did not reduce hyperlipidaemia in transgenic mice overexpressing human APOC1, irrespective of both the level of transgenic expression and the presence of the LDLR, whereas Ad-VLDLR treatment did reverse hyperlipidaemia in LDLR-/- and wild-type mice. On the other hand, Ad-LDLR treatment strongly decreased plasma lipid levels in these APOC1 transgenic mice. These results suggest that apoC1 inhibits the clearance of lipoprotein particles via the VLDLR, but not via the LDLR. This hypothesis is corroborated by in vitro binding studies. Chinese hamster ovary (CHO) cells expressing the VLDLR (CHO-VLDLR) or LDLR (CHO-LDLR) bound less APOC1 transgenic VLDL than wild-type VLDL. Intriguingly, however, enrichment with apoE enhanced dose-dependently the binding of wild-type VLDL to CHO-VLDLR cells (up to 5-fold), whereas apoE did not enhance the binding of APOC1 transgenic VLDL to these cells. In contrast, for binding to CHO-LDLR cells, both wild-type and APOC1 transgenic VLDL were stimulated upon enrichment with apoE. From these studies, we conclude that apoC1 specifically inhibits the apoE-mediated binding of triacylglycerol-rich lipoprotein particles to the VLDLR, whereas apoC1-enriched lipoproteins can still bind to the LDLR. The variability in specificity of these lipoprotein receptors for apoC1-containing lipoprotein particles provides further evidence for a regulatory role of apoC1 in the delivery of lipoprotein constituents to different tissues on which these receptors are located.  (+info)

Adenovirus mediated p53 tumour suppressor gene therapy for human gastric cancer cells in vitro and in vivo. (5/9136)

BACKGROUND/AIMS: Gastric cancer is one of the most prevalent forms of cancer in East Asia. Point mutation of the p53 gene has been reported in more than 60% of cases of gastric cancer and can lead to genetic instability and uncontrolled cell proliferation. The purpose of this investigation was to evaluate the potential of p53 gene therapy for gastric cancer. METHODS: The responses of human gastric cancer cell lines, MKN1, MKN7, MKN28, MKN45, and TMK-1, to recombinant adenoviruses encoding wild type p53 (AdCAp53) were analysed in vitro. The efficacy of the AdCAp53 treatment for MKN1 and MKN45 subcutaneous tumours in nude mice was assessed in vivo. RESULTS: p53-specific growth inhibition was observed in vitro in two of four gastric cancer cell lines with mutated p53, but not in the wild type p53 cell line. The mechanism of the killing of gastric cancer cells by AdCAp53 was found, by flow cytometric analysis and detection of DNA fragmentation, to be apoptosis. In vivo studies showed that the growth of subcutaneous tumours of p53 mutant MKN1 cells was significantly inhibited by direct injection of AdCAp53, but no significant growth inhibition was detected in the growth of p53 wild type MKN45 tumours. CONCLUSIONS: Adenovirus mediated reintroduction of wild type p53 is a potential clinical utility in gene therapy for gastric cancers.  (+info)

Viral gene delivery selectively restores feeding and prevents lethality of dopamine-deficient mice. (6/9136)

Dopamine-deficient mice (DA-/- ), lacking tyrosine hydroxylase (TH) in dopaminergic neurons, become hypoactive and aphagic and die by 4 weeks of age. They are rescued by daily treatment with L-3,4-dihydroxyphenylalanine (L-DOPA); each dose restores dopamine (DA) and feeding for less than 24 hr. Recombinant adeno-associated viruses expressing human TH or GTP cyclohydrolase 1 (GTPCH1) were injected into the striatum of DA-/- mice. Bilateral coinjection of both viruses restored feeding behavior for several months. However, locomotor activity and coordination were partially improved. A virus expressing only TH was less effective, and one expressing GTPCH1 alone was ineffective. TH immunoreactivity and DA were detected in the ventral striatum and adjacent posterior regions of rescued mice, suggesting that these regions mediate a critical DA-dependent aspect of feeding behavior.  (+info)

Rescue of diabetes-related impairment of angiogenesis by intramuscular gene therapy with adeno-VEGF. (7/9136)

Diabetes is a major risk factor for coronary and peripheral artery diseases. Although diabetic patients often present with advanced forms of these diseases, it is not known whether the compensatory mechanisms to vascular ischemia are affected in this condition. Accordingly, we sought to determine whether diabetes could: 1) impair the development of new collateral vessel formation in response to tissue ischemia and 2) inhibit cytokine-induced therapeutic neovascularization. Hindlimb ischemia was created by femoral artery ligation in nonobese diabetic mice (NOD mice, n = 20) and in control C57 mice (n = 20). Hindlimb perfusion was evaluated by serial laser Doppler studies after the surgery. In NOD mice, measurement of the Doppler flow ratio between the ischemic and the normal limb indicated that restoration of perfusion in the ischemic hindlimb was significantly impaired. At day 14 after surgery, Doppler flow ratio in the NOD mice was 0.49+/-0.04 versus 0.73+/-0.06 for the C57 mice (P< or =0.005). This impairment in blood flow recovery persisted throughout the duration of the study with Doppler flow ratio values at day 35 of 0.50+/-0.05 versus 0.90+/-0.07 in the NOD and C57 mice, respectively (P< or =0.001). CD31 immunostaining confirmed the laser Doppler data by showing a significant reduction in capillary density in the NOD mice at 35 days after surgery (302+/-4 capillaries/mm2 versus 782+/-78 in C57 mice (P< or =0.005). The reduction in neovascularization in the NOD mice was the result of a lower level of vascular endothelial growth factor (VEGF) in the ischemic tissues, as assessed by Northern blot, Western blot and immunohistochemistry. The central role of VEGF was confirmed by showing that normal levels of neovascularization (compared with C57) could be achieved in NOD mice that had been supplemented for this growth factor via intramuscular injection of an adenoviral vector encoding for VEGF. We conclude that 1) diabetes impairs endogenous neovascularization of ischemic tissues; 2) the impairment in new blood vessel formation results from reduced expression of VEGF; and 3) cytokine supplementation achieved by intramuscular adeno-VEGF gene transfer restores neovascularization in a mouse model of diabetes.  (+info)

Adenoviral gene transfer of the human V2 vasopressin receptor improves contractile force of rat cardiomyocytes. (8/9136)

BACKGROUND: In congestive heart failure, high systemic levels of the hormone arginine vasopressin (AVP) result in vasoconstriction and reduced cardiac contractility. These effects are mediated by the V1 vasopressin receptor (V1R) coupled to phospholipase C beta-isoforms. The V2 vasopressin receptor (V2R), which promotes activation of the Gs/adenylyl cyclase system, is physiologically expressed in the kidney but not in the myocardium. Expression of a recombinant V2R (rV2R) in the myocardium could result in a positive inotropic effect via the endogenous high concentrations of AVP in heart failure. METHODS AND RESULTS: A recombinant adenovirus encoding the human V2R (Ad-V2R) was tested for its ability to modulate the cardiac Gs/adenylyl cyclase system and to potentiate contractile force in rat ventricular cardiomyocytes and in H9c2 cardiomyoblasts. Ad-V2R infection resulted in a virus concentration-dependent expression of the transgene and led to a marked increase in cAMP formation in rV2R-expressing cardiomyocytes after exposure to AVP. Single-cell shortening measurements showed a significant agonist-induced contraction amplitude enhancement, which was blocked by the V2R antagonist, SR 121463A. Pretreatment of Ad-V2R-infected cardiomyocytes with AVP led to desensitization of the rV2R after short-term agonist exposure but did not lead to further loss of receptor function or density after long-term agonist incubation, thus demonstrating resistance of the rV2R to downregulation. CONCLUSIONS: Adenoviral gene transfer of the V2R in cardiomyocytes can modulate the endogenous adenylyl cyclase-signal transduction cascade and can potentiate contraction amplitude in cardiomyocytes. Heterologous expression of cAMP-forming receptors in the myocardium could lead to novel strategies in congestive heart failure by bypassing the desensitized beta-adrenergic receptor signaling.  (+info)

Mycoplasma hyopneumoniae causes severe economic losses to the swine industry worldwide and the prevention of its related disease, enzootic porcine pneumonia, remains a challenge. The P97 adhesin protein of M. hyopneumoniae should be a good candidate for the development of a subunit vaccine because antibodies produced against P97 could prevent the adhesion of the pathogen to the respiratory epithelial cells in vitro. In the present study, a P97 recombinant replication-defective adenovirus (rAdP97c) subunit vaccine efficiency was evaluated in pigs. The rAdP97c vaccine was found to induce both strong P97 specific humoral and cellular immune responses. The rAdP97c vaccinated pigs developed a lower amount of macroscopic lung lesions (18.5 ± 9.6%) compared to the unvaccinated and challenged animals (45.8 ± 11.5%). rAdP97c vaccine reduced significantly the severity of inflammatory response and the amount of M. hyopneumoniae in the respiratory tract. Furthermore, the average daily weight gain was ...
In vivo gene transfer and expression in normal uninjured blood vessels using replication-deficient recombinant adenovirus vectors Academic Article ...
TY - JOUR. T1 - Enhancement of in vivo adenovirus-mediated gene transfer and expression by prior depletion of tissue macrophages in the target organ. AU - Wolff, Gerhard. AU - Worgall, Stefan. AU - Van Rooijen, Nico. AU - Song, Wen Ru. AU - Harvey, Ben Gary. AU - Crystal, Ronald G.. PY - 1997/1/1. Y1 - 1997/1/1. N2 - Based on the hypothesis that tissue macrophages present an obstacle for adenovirus (Ad) vector-mediated gene transfer to internal organs, this study evaluated the consequences of transient depletion of Kupffer cells on subsequent transfer of the Ad vector genome and Ad vector-directed gene expression in the livers of experimental animals. Depletion of Kupffer cells in mice by intravenous administration of multilamellar liposomes containing dichloromethylene-bisphosphonate permitted subsequent intravenous administration of an Ad vector to provide a higher input of recombinant adenoviral DNA to the liver, an absolute increase in transgene expression, and a delayed clearance of the ...
TY - JOUR. T1 - Inhibition of repair of radiation-induced DNA damage enhances gene expression from replication-defective adenoviral vectors. AU - Hingorani, Mohan. AU - White, Christine L.. AU - Merron, Andrew. AU - Peerlinck, Inge. AU - Gore, Martin E.. AU - Slade, Andrew. AU - Scott, Simon D.. AU - Nutting, Christopher M.. AU - Pandha, Hardev S.. AU - Melcher, Alan A.. AU - Vile, Richard Geoffrey. AU - Vassaux, Georges. AU - Harrington, Kevin J.. PY - 2008/12/1. Y1 - 2008/12/1. N2 - Radiation has been shown to up-regulate gene expression from adenoviral vectors in previous studies. In the current study, we show that radiation-induced dsDNA breaks and subsequent signaling through the mitogen-activated protein kinase (MAPK) pathway are responsible, at least in part, for this enhancement of transgene expression both in vitro and in vivo. Inhibitors of ataxia-telangiectasia-mutated, poly(ADP-ribose) polymerase-mutated, and DNA-dependent protein kinase (DNA-PK)-mediated DNA repair were shown to ...
Staining for LacZ 5 days after intraocular injection of AdLacZ.10 in adult wild-type mice and at various times after injection in transgenic mice with increased expression of PDGF in the retina. Mice were given an intravitreous injection of 5 × 108 (A-E, J-P) or a subretinal injection of 107 (F-I) AdLacZ.10 particles. After 5 days, or as otherwise indicated, ocular sections and retinal and choroidal wholemounts were stained for LacZ. (A) Albino BALB/c mice showed staining in the corneal endothelium, the trabecular meshwork, the iris pigmented epithelium, and the ciliary body (bar, 200 μm). (B) Flatmounts of iris from albino BALB/c mice showed diffuse dark staining throughout the entire iris. (C.) Retinal wholemounts from adult C57BL/6 mice showed scattered focal staining throughout the retina, with more intense staining at the optic nerve. (D, E) Ocular sections from adult C57BL/6 mice showed prominent staining in and around the optic nerve and focal staining of cells in the inner nuclear ...
Title: Recent Advances in Adenovirus-mediated Gene Therapy for Cerebral Ischemia. VOLUME: 3 ISSUE: 1. Author(s):Makoto Masumura and Ryuji Hata. Affiliation:Suntory Biomedical Research Limited, 1-1-1, Wakayama-dai, Shimamoto-cho, Mishima-gun, Osaka 618- 8503, Japan. Keywords:cerebral ischemia, cerebral infarction, gene therapy, adenovirus vector. Abstract: Cerebral ischemia induces many degenerative cellular reactions, including the release of excitatory amino acids, the formation of oxygen free radicals, Ca2+ overload, the activation of several cellular enzyme systems such as Ca2+ dependent proteases, and the initiation of genomic responses that can affect the tissue outside the area of reduced blood flow. Furthermore, increasing evidence indicates that apoptosis contributes to the death of brain cells following cerebral ischemia. Several studies have shown that cerebral ischemia alters the expression of genes, some of which may play protective or harmful roles. Although many genes have the ...
TY - JOUR. T1 - In vivo studies of adenovirus-based p53 gene therapy for ovarian cancer. AU - Von Gruenigen, Vivian E.. AU - Santoso, Joseph T.. AU - Coleman, Robert L.. AU - Muller, Carolyn Y.. AU - Miller, David Scott. AU - Mathis, J. Michael. PY - 1998/6. Y1 - 1998/6. N2 - Objectives. To test the safety, efficacy, and toxicity of gene therapy using wild-type p53-expressing adenovirus (Ad-CMV-p53) in a nude mouse model with intraperitoneal (ip) 2774 human ovarian cancer cell line that contains a p53 mutation. Study design. An initial study of adenovirus tolerance was determined in nude mice by a single ip injection of increasing doses of Ad- CMV-p53. Nude mice were implanted with an LD100 dose of 1 x 107 cells. To study the efficacy and specificity of Ad-CMV-p53 treatment, the mice received treatment with different adenovirus constructs. One group received Ad-CMV- p53 and another group received a control adenovirus construct, Ad-CMV-βgal. To study the treatment response to Ad-CMV-p53, the ...
Purification of recombinant adenovirus type 3 dodecahedric virus-like particles for biomedical applications using short monolithic columns
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Yang et al identified miR-206 in a microarray screen for upregulated miRNAs in neonatal rat cardiomyocytes overexpressing Yap. Previously, miR-206 had been shown to play an important role in skeletal muscle hypertrophy.11 Yang et al performed a series of in vitro experiments that led them to conclude that the Hippo/Yap pathway regulates cardiomyocyte hypertrophy and survival through the actions of miR-206. Adenoviral-mediated overexpression of Yap in neonatal rat cardiomyocytes led to increased miR-206 expression, whereas overexpression of Mst1, a critical kinase in the Hippo pathway, led to decreased miR-206 expression. To draw a functional connection between Yap and miR-206, the authors show that adenoviral-mediated overexpression of miR-206 or Yap in neonatal rat cardiomyocytes produced similar changes in cardiomyocyte hypertrophy and cell viability after treatment with the apoptosis inducer chelerythrine. Combined loss of miR-206 and overexpression of Yap in vitro abrogated the phenotypic ...
Vaccines that have benefited from the use of adjuvants span multiple classes, such as subunit vaccines, vaccines employing viral vectors and DNA vaccines. For example, the addition of adjuvants to administered peptide antigens such as gp120 can augment immune activity in vivo, resulting in durable responses postvaccination [49]. The use of viral vectors as a means of delivering an antigen of interest for vaccination continues to have wide popularity both in academia [50-53] as well as industry [54-56]. Employing viruses such as Modified Vaccinia Virus Ankara (MVA) allows for delivery of antigens into the cytoplasm of target cells, thus providing expression through MHC Class I and subsequent generation of CTL responses [57,58]. In addition to MVA, recombinant adenoviruses are being employed more often as viral vectors in vaccination [51,52,54-57]. While there are a number of adenovirus serotypes, recombinant replication-defective adenovirus serotype 5 (rAd5) is the most commonly employed variant ...
The majority of breast cancers are oestrogen dependent and although current treatment strategies have improved, approximately 50% of the patients will develop metastasis. New treatments that result in long-term systemic immunity are therefore being developed. We have previously shown that adenoviral gene transfer of B7-I/IL-2 to murine breast cancer induces a high rate of complete turnout regression and systemic immunity. Since oestrogens not only affect breast cancer but also have been shown to modulate immune function and secretion of immune-regulatory cytokines, we explored whether administration of oestradiol altered the immune response induced by an adenoviral vector expressing B7-I/IL-2. An oestrogen-dependent murine breast cancer tumour was used in ovariectomised mice, supplemented either oestradiol or placebo. We report the somewhat unexpected finding that intratumoral injection of adenovirus expressing B7-I/IL-2 induces complete turnout regression in 76% of oestradiol-supplemented mice, ...
TY - JOUR. T1 - Reversal of potassium channel deficiency in cells from failing hearts by adenoviral gene transfer. T2 - A prototype for gene therapy for disorders of cardiac excitability and contractility. AU - Nuss, H. B.. AU - Johns, D. C.. AU - Kääb, S.. AU - Tomaselli, G. F.. AU - Kass, David A. AU - Lawrence, J. H.. AU - Marban, E.. PY - 1996. Y1 - 1996. N2 - Heart failure is a common, often lethal disorder in which conventional pharmacologic strategies have achieved limited success. Failing hearts exhibit a delay of electrical repolarization which predisposes to fatal arrhythmias. To explore the feasibility of gene therapy for this condition, we isolated myocytes from normal and failing dog hearts and quantified electrophysiologic and contractile parameters in primary culture. Action potentials were prolonged in failing cells as a result of diminished potassium currents. Exposure to AdShK, an adenovirus that overexpresses potassium channels, reversed the action potential prolongation of ...
Adenoviral System, Tet-On 3G Inducible. Very tight control of gene expression combined with the most advanced adenoviral gene delivery technology. cloning is even simpler than standard plasmid cloning.
Adenoviral System, Tet-On 3G Inducible. Very tight control of gene expression combined with the most advanced adenoviral gene delivery technology. cloning is even simpler than standard plasmid cloning.
Adenoviruses commonly infect humans, causing colds, flu-like symptoms and sometimes even death, but now UC San Francisco researchers have discovered that a new species of adenovirus can spread from primate to primate, and ...
In the current study, we tested if upregulation of CFTR function, via adenoviral-mediated overexpression, alters alveolar active Na+ transport in rodents. To affect this goal, we used previously tested adenovectors and established delivery strategies that produce transgene expression in both type 1 and 2 alveolar epithelial cells in rats and mice (Figure 1C).16 Data from other groups indicates that the β2AR is present in both type 1 and 2 epithelial cells,30,31 thus it is likely that the methods used in these experiments result in concomitant expression of CFTR and β2AR in both types of alveolar epithelial cells.. The functional effects of CFTR gene transfer in rats were assessed by measuring the capacity of the lung to clear fluid instilled into the lung using a well-described isolated rat lung model.10 This experimental design is based on the principal that AFC is an index of active, vectorial Na+ transport by alveolar epithelial cells. Using this approach, we observed that overexpression of ...
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A major limitation of cancer gene therapy strategies is the inability of replication-defective Ad vectors to disseminate throughout a solid tumor. A number of groups are seeking to address this problem by using Ads that replicate in cancer cells, thereby lysing the cells and releasing viral progeny that spread to neighboring cells. The potency of this novel class of anticancer agents will therefore depend on the efficiency of dissemination of the virus throughout the tumor.. In this study, we have investigated the hypothesis that the efficacy of replicating Ads could be restricted by poor dissemination of the viral progeny due to the inability to infect tumor cells expressing low levels of the primary Ad receptor. To address this issue, we have used a pair of tumor cell lines that differ only in the expression of a primary receptor for Ad5. We demonstrated that the oncolytic potency of a replicating Ad was significantly greater in the receptor-positive cell line, both in monolayers of cells in ...
Cat.No.AD00697ZDescriptionAdenovirus with ORF of adenylate cyclase 9 (ADCY9) with C terminal Flag and His tag.InsertADCY9Product TypeAdenoviral particleShippingDry iceSize/Form1
Preferred Name: Adenovirus-mediated Human Interleukin-12 INXN-2001 Plus Activator Ligand INXN-1001 Definition: A replication incompetent adenovirus encoding the human pro-inflammatory cytokine interleukin-12 (IL-12) (INXN-2001) in combination with the proprietary activator ligand INXN-1001, with potential immunomodulating and antineoplastic activities. Production of IL-12 is controlled by an inducible DNA element that allows transcription initiation only in the presence of the ligand inducer. Upon intratumoral administration of INXN-2001 and oral administration of INXN-1001, INXN-1001 is able to induce expression of IL-12 from INXN-2001. IL-12 expressed by the adenovirus may activate the immune system by promoting the activation of natural killer cells (NKs), inducing secretion of interferon-gamma and inducing cytotoxic T cell responses against tumor cells, which may result in immune-mediated tumor cell death and inhibition of tumor cell proliferation. Display Name: Adenovirus-mediated Human ...
Molecular Cloning, also known as Maniatis, has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential.
Rs instructions. Each recombinant adenovirus was selected after three rounds of plaque purification in HEK293 cells and was separately identified by PCR. The
TY - JOUR. T1 - Retrograde transfer of replication deficient recombinant adenovirus vector in the central nervous system for tracing studies. AU - Kuo, Hui. AU - Ingram, Donald K.. AU - Crystal, Ronald. AU - Mastrangeli, Andrea. PY - 1995/12/24. Y1 - 1995/12/24. N2 - We assessed the application of a replication deficient recombinant adenovirus vector as a retrograde tracer in neural pathway studies. The adenovirus vector, Ad.RSVBgal, containing the intracellular marker gene, β-galactosidase, was injected directly into the laterodorsal striatum of rats. The retrograde transport of the vector from the injection site was clearly visible in the cerebral cortex, thalamic nucleus, and substantia nigra. No evidence for anterograde transport of the vector was found. When the vector was injected into the genu of the corpus callosum, little uptake of the vector by fibers was noted which suggested that uptake by fibers-of-passage should not be a problem in tracing studies. The present study demonstrates ...
Title: Transductional Targeting with Recombinant Adenovirus Vectors. VOLUME: 2 ISSUE: 3. Author(s):Valerie Legrand, Philippe Leissner, Arend Winter, Majid Mehtali and Monika Lusky. Affiliation:CAREXS.A., 11 rue Humann, 67000 Strasbourg, France. Keywords:Adenovirus Vectors, TROPISM, Fiber Protein, Monoclonal antibody. Abstract: Replication-deficient adenoviruses are considered as gene delivery vectors for the genetic treatment of a variety of diseases. The ability of such vectors to mediate efficient expression of therapeutic genes in a broad spectrum of dividing and non-dividing cell types constitutes an advantage over alternative gene transfer vectors. However, this broad tissue tropism may also turn disadvantageous when genes encoding potentially harmful proteins (e.g. cytokines, toxic proteins) are expressed in surrounding normal tissues. Therefore, specific restrictions of the viral tropism would represent a significant technological advance towards safer and more efficient gene delivery ...
OBJECTIVES To investigate the validity of catalase recombinant adenovirus on the treatment of oxidative cataract. METHODS The coding sequence of catalase was cloned and the catalase recombinant adenovirus was constructed. The expression time course of catalase gene in rat lens infected by recombinant adenovirus was determined by Western blotting. Cultured rat lens were randomly divided into 3 groups: the control group, the group treated by hydrogen peroxide and the group treated by hydrogen peroxide combined with catalase recombinant adenovirus. The transparence and apoptosis ratio of lens on the time points of 6, 12, 18, 24 hours were determined by image analysis and double colour flowcytometry. RESULTS The coding sequence of catalase was cloned and recombinant adenovirus was successfully constructed. The expression of catalase in cultured rat lens infected by recombinant adenovirus reached peak point on 9 hours post infection and maintained the level in the whole experiment period. The
Improvement of transduction and augmentation of cytotoxicity are crucial for adenoviruses (Ad)-mediated gene therapy for cancer. Down-regulated expression of type 5 Ad (Ad5) receptors on human tumors hampered Ad-mediated transduction. Furthermore, a role of the p53 pathways in cytotoxicity mediated by replication-competent Ad remained uncharacterized. We constructed replication-competent Ad5 of which the E1 region genes were activated by a transcriptional regulatory region of the midkine or the survivin gene, which is expressed preferentially in human tumors. We also prepared replication-competent Ad5 which were regulated by the same region but had a fiber-knob region derived from serotype 35 (AdF35). We examined the cytotoxicity of these Ad and a possible combinatory use of the replication-competent AdF35 and Ad5 expressing the wild-type p53 gene (Ad5/p53) in esophageal carcinoma cells. Expression levels of molecules involved in cell death, anti-tumor effects in vivo and production of viral progenies
Granulocyte macrophage-colony stimulating factor (GM-CSF) is a hematopoietic growth factor involved in differentiation, survival and activation of myeloid and non-myeloid cells with important implications for lung antibacterial immunity. Here we examined the effect of pulmonary adenoviral vector-mediated delivery of GM-CSF (AdGM-CSF) on anti-mycobacterial immunity in M. bovis BCG infected mice. Exposure of M. bovis BCG infected mice to AdGM-CSF either applied on 6h, or 6h and 7days post-infection substantially increased alveolar recruitment of iNOS and IL-12 expressing macrophages, and significantly increased accumulation of IFNγpos T cells and particularly regulatory T cells (Tregs). This was accompanied by significantly reduced mycobacterial loads in the lungs of mice. Importantly, diphtheria toxin-induced depletion of Tregs did not influence mycobacterial loads, but accentuated immunopathology in AdGM-CSF-exposed mice infected with M. bovis BCG. Together, the data demonstrate that AdGM-CSF ...
TY - JOUR. T1 - Postischemic gene transfer of midkine, a neurotrophic factor, protects against focal brain ischemia. AU - Takada, J.. AU - Ooboshi, H.. AU - Ago, T.. AU - Kitazono, T.. AU - Yao, H.. AU - Kadomatsu, K.. AU - Muramatsu, T.. AU - Ibayashi, S.. AU - Iida, M.. PY - 2005/3/1. Y1 - 2005/3/1. N2 - Gene therapy may be a promising approach for treatment of brain ischemia. In this study, we examined the effect of postischemic gene transfer of midkine, a heparin-binding neurotrophic factor, using a focal brain ischemia model with the photothrombotic occlusion method. At 90 min after induction of brain ischemia in spontaneously hypertensive rats, a replication-deficient recombinant adenovirus encoding mouse midkine (AdMK, n = 7) or a control vector encoding β-galactosidase (Adβgal, n = 7) was injected into the lateral ventricle ipsilateral to ischemia. At 2 days after ischemia, we determined infarct volume by 2,3,5-triphenyltetrazolium chloride staining. There were no significant ...
Control of the HIV pandemic can only be achieved with the development of a safe and effective preventive HIV vaccine. A vaccine that will prevent HIV infection will elicit a strong immune response from both CD4 and CD8 cells. Recombinant adenovirus serotype vectors have been shown to elicit just such a response. The purpose of this study is to determine the safety and immunogenicity of the recombinant adenovirus serotype 5 preventive HIV-1 vaccine.. This study will last 18 to 24 months. Participants will be randomly assigned to one of four arms that will receive vaccine or placebo administered via intramuscular injection. Participants in Arms 1, 2, and 3 will all receive 3 injections. Participants in Arm 4 will receive one injection. For most participants, there will be 10 study visits in this study; for participants in Arm 4, there will be only 7 visits. For Arms 1, 2, and 3, study visits will occur at baseline and on Days 0, 14, 28, 42, 56, 168, 182,196, and 365. Participants in Arms 1, 2, and ...
Adenoviral Vectors for Gene treatment, moment Edition offers special, entire assurance of the gene supply cars which are in line with the adenovirus thats rising as an immense instrument in gene remedy. those fascinating new healing brokers have nice capability for the therapy of illness, making gene remedy a fast-growing box for learn. This ebook provides themes starting from the fundamental biology of adenoviruses, during the building and purification of adenoviral vectors, state-of-the-art vectorology, and using adenoviral vectors in preclinical animal types, with ultimate attention of the regulatory concerns surrounding human medical gene remedy trials. This extensive scope of knowledge offers a superb evaluate of the sector, permitting the reader to achieve an entire figuring out of the advance and use of adenoviral vectors.. ...
Intraperitoneal (i.p.) recurrence of cisplatin-refractory and p53 mutant ovarian cancer is a major clinical problem, despite surgery and chemotherapy. dl1520 (ONYX-015) is an E1B-55 kDa gene-deleted adenovirus engineered selectively to replicate in and destroy cancer cells lacking functional p53. Ho …
Recombinant adenovirus vectors are promising, highly characterized platforms from which novel vaccines can be produced. Conventional Ad5[E1−] vaccines possess the ability to promote strong immunologic responses against their expressed transgenes, but the same properties also trigger host antiviral responses, which can lead to increased toxicity, limited persistence, and decreased efficacy in the face of preexisting anti-Ad5 immunity (7, 28, 29, 34, 46). Alternatively, multiply deleted Ad5[E1−,E2b−] vaccines have been found to overcome several of these obstacles. Head-to-head comparisons of traditional Ad5[E1−] vaccines and E2b gene-deleted, Ad5[E1−,E2b−] vaccines have shown that the latter produce virtually no hepatotoxicity after systemic administration (29), persist and express transgenes for longer durations than Ad5[E1−] vaccines (28, 34), and remain therapeutically efficacious in hosts harboring substantial preexisting anti-Ad5 immunity (5, 14-16, 30).. It has been largely ...
Recombinant adenoviruses currently are used for a variety of purposes, including gene transfer in vitro, vaccination in vivo, and gene therapy (1-4). Several features of adenovirus biology have made such viruses the vectors of choice for certain of these applications. For example, adenoviruses transfer genes to a broad spectrum of cell types, and gene transfer is not dependent on active cell division. Additionally, high titers of viruses and high levels of transgene expression generally can be obtained.. Decades of study of adenovirus biology have resulted in a detailed picture of the viral life cycle and the functions of the majority of viral proteins (5, 6). The genome of the most commonly used human adenovirus (serotype 5) consists of a linear, 36-kb, double-stranded DNA molecule. Both strands are transcribed and nearly all transcripts are heavily spliced. Viral transcription units are conventionally referred to as early (E1, E2, E3, and E4) and late, depending on their temporal expression ...
The plasminogen activation (PA) system is involved in vascular remodelling. Modulating its activity in vascular cells might be a way to interfere in processes such as angiogenesis and restenosis. Adenoviral vectors have become a favourable tool for direct gene transfer into vascular cells. In the interest of using adenoviral vectors to modulate plasminogen activator activity and endothelial and smooth muscle cell migration, we studied the effects of endothelial and smooth muscle cell transduction in vitro and in the umbilical vein ex vivo with a replication-defective adenoviral vector containing the β-galactosidase gene (AdCMVLacZ). Segments of the umbilical vein were infected with AdCMVLacZ (109-1010 pfu/ml). After 48 h strong β-galactosidase expression could be observed in the vessel wall, which was restricted to the endothelial layer. Although some heterogeneity in the transduction throughout the vessel could be seen, β-galactosidase expression was detectable for 21 days in explant. ...
A recombinant nucleic acid used for the production of a defective adenovirus containing an inserted sequence coding for a cytokine under the control of a promoter in the genomic sequence of the recombinant adenovirus. This recombinant adenovirus is useful in the preparation of anti-tumoral drugs which can be directly injected into the tumor of the host.
A simplified Ebola vaccine that consists of a modified GP protein (which is well-tolerated by human cells even at high concentrations) in a replication-defective adenoviral vector protects macaques.
La terapia génica consiste en la manipulación y utilización de material genético para el tratamiento de patologías. No obstante, esta estrategia requiere el uso de vectores de terapia génica para transportar el material genético al tejido diana de forma eficiente. Los vectores de terapia génica más comunes son los basados en el adenovirus humano de serotipo 5 (Ad5), porque, respecto a otros serotipos, el Ad5 tiene como ventaja su bioseguridad y facilidad de producción. Sin embargo, para que una célula sea transducida por el Ad5 ha de expresar un receptor reconocible por el Ad5, principalmente la proteína CAR, al que se une a través de su proteína fiber. El fiber de otros serotipos de adenovirus se une a receptores diferentes, permitiendo así la transducción de tipos celulares alternativos a los transducidos por el Ad5. De esta manera, una de las estrategias utilizadas para obtener un vector Ad5 con el tropismo de un serotipo diferente, es la pseudotipación del adenovirus, que ...
Wild-type p53 is involved in several aspects of cell cycle control and suppression of transformation, inducing either apoptosis or G1 block in cell cycle progression. Using a recombinant adenovirus containing the wild-type p53 cDNA, the biological effects of the newly expressed wild-type p53 protein were examined in six human glioma cell lines. Three cell lines (U-251 MG, U-373 MG, and A-172) expressed endogenous mutant p53, and the other three (U-87 MG, EFC-2, and D54 MG) expressed wild-type p53. The restoration of normal p53-encoded protein in the mutant cell lines induced apoptosis as assessed by morphological studies using nuclear staining, electron microscopy, and flow cytometric assays. In wild-type p53 cell lines, however, the overexpression of wild-type p53 did not result in apoptosis but inhibited cellular proliferation rather drastically and modified the neoplastic phenotype. Differential effects suggest two pathways for glioma oncogenesis and a possible therapeutic strategy.. ...
Dendritic cells (DCs) are professional antigen presenting cells that are being considered as potential immunotherapeutic agents to promote host immune responses against tumor antigens. The use of such modified antigen-presenting cells for research or therapeutic have been limited by several factors, including maintaining DCs in a highly activated state, efficient transduction and expression, stable expression, identification of appropriate tumor-associated antigens, and absence of unintended functional changes or cytotoxicity. In this study, the feasibility of using CD34-DCs for tumor immunotherapy after transduction with a recombinant adenovirus containing HBsAg gene (AdVHBsAg), an HCC-associated antigen, was investigated. The gene transfer with recombinant adenovirus vectors (AdV) can obtained high levels of stable expression of HBsAg and its efficiency was increased in a multiplicity of infection (MOI)-dependent manner. Moreover, the AdVHBsAg infection had no appreciable effect on apoptosis ...
Chronic obstructive pulmonary disease (COPD) and lung cancer are major causes of death in the world. Exposure to tobacco smoke presents the main factor in the development of COPD. Epidemiological research has shown that COPD represents, regardless of smoking status, the biggest risk factor for developing lung cancer. The genetic basis of the association of these pathological entities is unknown. The regulation of the immune response plays an important role in maintaining homeostasis. Toll-like receptors (TLR) are important for initiating immunity by recognising the sequences associated with the pathogen and inducing signal pathways in the host as an antimicrobial response. Previous researches have shown that the genetic variant of TLR5, N592S gene, is associated with COPD and lung cancer. The aim of this research was to construct a replication-defective adenoviral vector by introducing a gene for TLR5wt and TLR5N592S into the adenovirus type 5 (Ad5) backbone that would allow the insertion of ...
Biological Sciences Shirley, Cat.No. AD00876Z Description Adenovirus with ORF of aminolevulinate dehydratase (ALAD) with C terminal Flag and His tag....
Dendritic cells (DCs) modified by some immunomodulatory genes can stimulate a strong antitumor immunity and improve the treatment of tumor cells on the condition that the sources of tumor-associated antigens (TAAs) are available. IL-6, a pleotropic cytokine, has been found to inhibit CD4+25+ regulatory T (Treg)-cell-mediated immune suppression and decrease activation-induced cell death (AICD) without interfering the process of T-cell activation. To enhance DC-based cancer vaccine, we engineered DCs to express transgene IL-6.. We constructed a fiber-modified recombinant adenovirus vector AdVIL-6 expressing IL-6, infected DCs with AdVIL-6, and then investigated the efficacy of antitumor immunity induced by vaccination with DCs engineered to express IL-6 transgene. We demonstrated that DCs infected with the recombinant adenovirus AdVIL-6 induced DC maturation by up-regulation of the expression of MHC class U (Iab), CD40, CD54 and CD80 expression. We also demonstrated that vaccination of OVA-pulsed ...
Many applications for human gene therapy would be facilitated by high levels and long duration of physiologic gene expression. Adenoviral vectors are frequently used for gene transfer because of their high cellular transduction efficiency in vitro and in vivo. Expression of viral proteins and the lo …
Described are vaccines comprising recombinant vectors, such as recombinant adenoviruses. The vectors comprise heterologous nucleic acids encoding at least two antigens from one or more tuberculosis-c
13:2; potential epub Adenovirus notions; tenure bhakti 1? On the epub Adenovirus Methods and of this interest, are above under form. 1282 An epub Adenovirus Methods and Protocols: Adenoviruses, Ad study read by Porten - Szubin 1987:187.
A quantum dot method for highly efficient labelling of single adenoviral particles is developed. The technique has no impact on viral fitness and allows the imaging and tracking of virus binding and internalisation events using a variety of techniques including imaging cytometry and confocal microscopy. The method is applied to characterise the tropism of different adenoviral vectors.. ...
... , information about the mechanism of Adenoviruses, Adeviral particle organisation and genome organisation of adenoviruses
As combining therapeutic agents with different action mechanisms may enhance efficacy, YSC-02, an oncolytic adenovirus with multi targets was loaded with five different genes, which were designed with the expectation that different action mechanisms would cooperate. In spite of concerns regarding many APIs, YSC-02 was constructed to be an adenovirus-based anti-cancer drug. By using our own established mouse model system suitable for efficient adenoviral infection and replication, immune activity as well as survival potential could be precisely estimated for anti-cancer drug efficacy. YSC-02 was designed to decrease tumor cell survival, metastasis and to increase tumor cell death, and immune activation. It is composed of five different target genes, including one fused form and two shRNAs, but each of these genes functions is closely linked to produce the maximal antitumor effect. YSC-02 is like an organic complex designed to be applied primarily to heterogeneous liver cancer and melanoma. The ...
This study was initiated with the original aim of assessing the consequences of the expression of the functional α2-subunit of the rat Na+-K+-ATPase in the neonatal rat cardiac myocytes lacking this isoform. It soon became evident, however, that only a truncated α2-subunit not likely to be functional was overexpressed in these cells. Because enzyme and receptor fragments may often act like inactive mutant variants and cause dominant negative inhibition (2, 10, 12, 23, 27, 36) we attempted to determine whether the expression of the α2-fragment impaired the function of endogenous Na+-K+-ATPase in the neonatal myocytes. Our findings clearly show that the ion transport function of Na+-K+-ATPase is indeed inhibited concomitant with the expression of the truncated α2-isoform and that this is accompanied by a significant reduction of the α1-protein content of the neonatal myocyte. Because the induced reduction of the α3-protein content is small, if any, and because it is established that α1 ...
nonenveloped: Adenoviridae. *Papillomaviridae. *Papovaviridae (obsolete). *Polyomaviridae. *genera: Rhizidiovirus. * ...
nonenveloped: Adenoviridae. *Papillomaviridae. *Papovaviridae (obsolete). *Polyomaviridae. *genera: Rhizidiovirus. * ...
Carter, G.R.; Wise, D.J. (2006). "Adenoviridae". A Concise Review of Veterinary Virology. Retrieved 2006-06-10. Ettinger, ...
m Adenoviridae‎; 09:18 . . (+13)‎ . . ‎. Tom.Reding. (talk , contribs)‎ (Add from=Q193447 to {{Taxonbar}}; WP:GenFixes on, ...
Animal adenoviridae explained. ...
Familie Adenoviridae. *Genus Atadenovirus. *Genus Aviadenovirus. *Genus Ichtadenovirus. *Genus Mastadenovirus (mit den Species ...
Adenoviridae Oncolytic adenovirus Lowe, SW; Ruley, HE (1993). "Stabilization of the p53 tumor suppressor is induced by ...
Examples include Herpesviridae, Adenoviridae, and Papovaviridae. There is only one well-studied example in which a class 1 ...
Adenoviridae. *Herpesviridae (skoldkopper, Epstein-Barr virus og mononukleose). *Mimiviridae Langkodede vira med tykke skaller ...
Mononegavirales adalah ordo virus RNA yang berada dalam filum Negarnaviricota dan kelas Monjiviricetes.[1] Nama Mononegavirales berasa dari bahasa Yunani μóνος [monos] yang merujuk pada genom untai tunggal pada sebagian besar ordo ini, bahasa Latin negare yang merujuk pada sifat sense-negatif genom virus, serta akhiran -virales yang menunjukkan ordo virus.[2] Anggota ordo ini yang dikenal di antaranya virus rabies dan virus Ebola yang dapat menyebabkan penyakit, baik pada manusia maupun hewan. ...
Famili Adenoviridae. *Famili Asfarviridae - includes African swine fever virus. *Famili Baculoviridae. *Famili ...
腺病毒科 Adenoviridae. *乳头瘤病毒科 Papillomaviridae ...
腺病毒科 Adenoviridae. *乳头瘤病毒科 Papillomaviridae ...
... is a genus of viruses, in the family Adenoviridae. Human, mammals, and vertebrates serve as natural hosts. There ... Group: dsDNA Order: Unassigned Family: Adenoviridae Genus: Mastadenovirus Bat mastadenovirus A Bat mastadenovirus B Bovine ...
... is a genus of viruses, in the family Adenoviridae. Fish serve as natural hosts. There is currently only one ... species in this genus: the type species Sturgeon ichtadenovirus A. Group: dsDNA Order: Unassigned Family: Adenoviridae Genus: ...
... is a genus of viruses, in the family Adenoviridae. Vertebrates serve as natural hosts. There are currently five ... species in this genus including the type species Frog siadenovirus A. Group: dsDNA Order: Unassigned Family: Adenoviridae Genus ...
The families Adenoviridae and Tectiviridae appear to be related structurally. Baculoviruses evolved from the nudiviruses 310 ... Phylogenetic analysis of these genes places the adenoviruses (Adenoviridae), bacteriophages (Caudovirales) and the plant and ... Some have circular genomes (Baculoviridae, Papovaviridae and Polydnaviridae) while others have linear genomes (Adenoviridae, ... Species of the order Herpesvirales and of the families Adenoviridae, Asfarviridae, Iridoviridae, Papillomaviridae, ...
... (Agamid AdV1) is a type of virus in the Adenoviridae family. The virus is widespread in captive populations ...
In addition, bronchitis caused by Adenoviridae may cause systemic and gastrointestinal symptoms as well. However, the coughs ...
... (aka pADV 1-5 or pADV A-C) is a member of the adenoviridae family. It causes gastrointestinal disease in ... Adenoviridae Bovine adenovirus Porcine Adenovirus, reviewed and published by WikiVet at http://en.wikivet.net/Porcine_ ...
... , also known as BAdV, is a member of the Adenoviridae family that causes disease in cattle. There are 10 ...
... and adenoviridae, mumps and enterovirus can also cause pneumonia. Another cause of CAP in this group is Chlamydia trachomatis, ...
... (Bt-AdV-TJM) is a novel species of the Mastadenovirus genus of the family Adenoviridae. It is a double ...
Adenoviridae,adenoviruses]] (5%),,ref name=Medscape /, [[ Virusi sinkytial i frymëmarrjes njerëzore,virusi]] sinkytial i [[ ...
... ic viruses are diseases mainly of the families of: Adenoviridae, Picornaviridae, Herpesviridae, Hepadnaviridae, ...
Adenoviruses (members of the family Adenoviridae) are medium-sized (90-100 nm), nonenveloped (without an outer lipid bilayer) ... Group: dsDNA Order: Unassigned Family: Adenoviridae Genus: Atadenovirus Bovine atadenovirus D Duck atadenovirus A Ovine ... Adenoviridae Adenovirus Research Discussion Group USAMRMC protects Soldiers against unseen enemy 3D macromolecular structures ... Adenoviridae can be divided into five genera: Mastadenovirus, Aviadenovirus, Atadenovirus, Siadenovirus, and Ichtadenovirus. ...
Classification of Adenoviridae can be complex. In humans, there are 57 accepted human adenovirus types (HAdV-1 to 57) in seven ... When not restricting the subject to human viruses, Adenoviridae can be divided into five genera: Mastadenovirus, Aviadenovirus ... Adenoviruses (members of the family Adenoviridae) are medium-sized (90-100 nm), nonenveloped (without an outer lipid bilayer) ... Retrieved from "https://en.wikipedia.org/w/index.php?title=Adenoviridae&oldid=898892844" ...
Adenoviruses, family Adenoviridae. ]. (n.d.). Retrieved from https://www.shutterstock.com/image-illustration/adenoviruses- ... family-adenoviridae-their-name-derives-208224640. Health , Cold virus to aid cancer patients. (2007, January 11). Retrieved ... S. (n.d.). ViralZone: Adenoviridae. Retrieved January 30, 2017, from http://viralzone.expasy.org/all_by_species/4.html ...
Adenoviridae ,- style="text-align:center; background:violet;" !Genera ,- , style="padding: 0 .5em;" , Aviadenovirus. ... Siadenovirus ,} Adenoviruses are viruses of the family Adenoviridae. They infect both humans and animals. Adenoviruses were ...
Cardiovascular gene therapy is a novel approach to the treatment of diseases such as congestive heart failure (CHF). Gene transfer to the heart would allow for the replacement of defective or missing cellular proteins that ...
Adenoviridae explanation free. What is Adenoviridae? Meaning of Adenoviridae medical term. What does Adenoviridae mean? ... Looking for online definition of Adenoviridae in the Medical Dictionary? ... Adenoviridae. Also found in: Dictionary, Thesaurus, Encyclopedia, Wikipedia.. Related to Adenoviridae: adenovirus, ... Adenoviridae , definition of Adenoviridae by Medical dictionary https://medical-dictionary.thefreedictionary.com/Adenoviridae ...
What is Adenoviridae infections? Meaning of Adenoviridae infections medical term. What does Adenoviridae infections mean? ... Looking for online definition of Adenoviridae infections in the Medical Dictionary? Adenoviridae infections explanation free. ... Adenoviridae. (redirected from Adenoviridae infections). Also found in: Encyclopedia. Ad·e·no·vi·ri·dae. (adĕ-nō-viri-dē), A ... Adenoviridae infections , definition of Adenoviridae infections by Medical dictionary https://medical-dictionary. ...
Adenoviridae Remove constraint Subject: Adenoviridae Start Over ... Adenoviridae; Italy; Show all 21 Subjects. Abstract:. ... AIMS ...
Antonyms for Adenoviridae. 2 words related to adenovirus: parainfluenza virus, animal virus. What are synonyms for Adenoviridae ... redirected from Adenoviridae). Also found in: Dictionary, Medical, Encyclopedia, Wikipedia.. Related to Adenoviridae: ... The family Adenoviridae was verified by the first reaction of a generic adenovirus-specific nested PCR (10).. New adenovirus in ... These results indicated that RV 1 must belong to the family Adenoviridae.. Isolation of novel adenovirus from fruit bat ( ...
Adenoviridae. In: Concise Review of Veterinary Virology Author(s):. Carter G.R. and ...
Impact Adenoviral Stool - Free download as PDF File (.pdf), Text File (.txt) or read online for free. Impacto de medicion de adenovirus en deposiciones de pacientes transplantados de medula osea
Carter, G.R.; Wise, D.J. (2006). "Adenoviridae". A Concise Review of Veterinary Virology. Retrieved 2006-06-10. Ettinger, ...
Adenoviridae Remove constraint Subject: Adenoviridae Start Over ... Adenoviridae; Italy; Show all 21 Subjects. Abstract:. ... AIMS ...
Adenoviridae. A family of double-stranded DNA viruses, collectively known as adenoviruses, which are icosahedrally symmetric, ...
nonenveloped: Adenoviridae. *Papillomaviridae. *Papovaviridae (obsolete). *Polyomaviridae. *genera: Rhizidiovirus. * ...
b. Adenoviruses belong to the family Adenoviridae and have been isolated from humans and animals. 2. Infections a. Respiratory ...
In yet other preferred embodiments, the virus includes, but is not limited to, Parvoviridae, Papovaviridae, Adenoviridae, ...
Familie Adenoviridae. *Genus Atadenovirus. *Genus Aviadenovirus. *Genus Ichtadenovirus. *Genus Mastadenovirus (mit den Species ...
Adenoviridae 11. Papillomaviridae and Polyomaviridae 12. Parvoviridae 13. Circoviridae 14. Retroviridae 15. Reoviridae 16. ...
Family Adenoviridae Family Polyomaviridae 7. Subviral Agents. Introduction Defective Interfering Viruses Satellites and ...
Family Adenoviridae. *chorea (animal disease). Chorea, in dogs, a disorder in which muscle spasms are prominent. It is usually ...
Family Adenoviridae 338. Family Papillomaviridae 343. Family Polyomaviridae 347. Family Parvoviridae 349 ...
Adenoviridae. AD. RCC. Unknown. Transfusion transmitted virus Anelloviridae. NAA. No data found. Unknown. ...
Adenoviridae. Genus. Mastadenovirus. Genus. Aviadenovirus. Genus. Atadenovirus. Genus. Siadenovirus. Genus. Ichtadenovirus. ...
Adenoviridae. Adenovirus/UT. /Gabon. 2010-2012. Human. +/-. Reoviridae. Rotavirus/UT. /Gabon. 2010-2012. Human. +/-. ...
  • En biologisk virus består af et genom af DNA eller RNA og et antal enzymer, der er indlejret i en proteinkappe (kaldet en kapsid ), eventuelt omkrandset af membrankappe . (wikipedia.org)
  • Virus kan ikke formere sig alene, men overtager i stedet for en værtscelle og underkaster værtscellens molekylære maskineri for at fremstille kopier af sig selv og således producere den næste generation. (wikipedia.org)
  • Derfor bliver virus typisk ikke betragtet som levende organismer, fordi de alene ikke kan formere sig eller lave stofskifte , men kræver en vært . (wikipedia.org)
  • Dog har nye genetiske undersøgelser ændret på denne opfattelse, da det har vist sig at virus nedstammer fra en fælles stamform med moderne celler og har været en faktor, der har præget evolutionen . (wikipedia.org)
  • Virus består af et genom og nogle gange et par enzymer , der er indlejret i en proteinkappe (kaldet en kapsid ), eventuelt omkranset af membrankappe (en lipid-membran, eng. (wikipedia.org)
  • Virus kan ikke formere sig alene, men overtager i stedet for en værtscelle, dvs. (wikipedia.org)
  • Correction: A previous version of this article said that Adenoviridae is a virus that causes the flu and pneumonia. (boston.com)
  • Adenoviridae is a virus that causes the common cold and pneumonia. (boston.com)